LOCUS       DAYLZO010000001        85558 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.001, whole genome shotgun sequence.
ACCESSION   DAYLZO010000001 DAYLZO010000000
VERSION     DAYLZO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 85558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.001"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1286
                     /locus_tag="ACGVJG_000001"
     CDS             <1..1286
                     /locus_tag="ACGVJG_000001"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFJ6555995.1"
                     /translation="GFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVENPIKPV
                     KVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPEKTMSDV
                     TIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAIGTTFEP
                     PTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQEDINTN
                     SGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGIPTEASI
                     LALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSAFKELKT
                     VILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPGSIRFRG
                     ISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            1333..1917
                     /locus_tag="ACGVJG_000002"
     CDS             1333..1917
                     /locus_tag="ACGVJG_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFJ6555996.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            1919..2338
                     /locus_tag="ACGVJG_000003"
     CDS             1919..2338
                     /locus_tag="ACGVJG_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFJ6555997.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(2619..2903)
                     /locus_tag="ACGVJG_000004"
     CDS             complement(2619..2903)
                     /locus_tag="ACGVJG_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFJ6555998.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(3255..3965)
                     /locus_tag="ACGVJG_000005"
     CDS             complement(3255..3965)
                     /locus_tag="ACGVJG_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFJ6555999.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(3962..5434)
                     /locus_tag="ACGVJG_000006"
     CDS             complement(3962..5434)
                     /locus_tag="ACGVJG_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFJ6556000.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGIVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            5572..6045
                     /locus_tag="ACGVJG_000007"
     CDS             5572..6045
                     /locus_tag="ACGVJG_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFJ6556001.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(6198..6749)
                     /locus_tag="ACGVJG_000008"
     CDS             complement(6198..6749)
                     /locus_tag="ACGVJG_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFJ6556002.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            6854..7384
                     /locus_tag="ACGVJG_000009"
     CDS             6854..7384
                     /locus_tag="ACGVJG_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFJ6556003.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(7867..8310)
                     /locus_tag="ACGVJG_000010"
     CDS             complement(7867..8310)
                     /locus_tag="ACGVJG_000010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6556004.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(8326..9486)
                     /locus_tag="ACGVJG_000011"
     CDS             complement(8326..9486)
                     /locus_tag="ACGVJG_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFJ6556005.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            9993..11693
                     /locus_tag="ACGVJG_000012"
     CDS             9993..11693
                     /locus_tag="ACGVJG_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556006.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(11795..12628)
                     /locus_tag="ACGVJG_000013"
     CDS             complement(11795..12628)
                     /locus_tag="ACGVJG_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFJ6556007.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(12932..15097)
                     /locus_tag="ACGVJG_000014"
     CDS             complement(12932..15097)
                     /locus_tag="ACGVJG_000014"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFJ6556008.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            15357..17651
                     /locus_tag="ACGVJG_000015"
     CDS             15357..17651
                     /locus_tag="ACGVJG_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFJ6556009.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            17644..18309
                     /gene="pgmB"
                     /locus_tag="ACGVJG_000016"
     CDS             17644..18309
                     /gene="pgmB"
                     /locus_tag="ACGVJG_000016"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFJ6556010.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            18302..19309
                     /locus_tag="ACGVJG_000017"
     CDS             18302..19309
                     /locus_tag="ACGVJG_000017"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFJ6556011.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            19331..20350
                     /locus_tag="ACGVJG_000018"
     CDS             19331..20350
                     /locus_tag="ACGVJG_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6556012.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(20631..21998)
                     /gene="nhaC"
                     /locus_tag="ACGVJG_000019"
     CDS             complement(20631..21998)
                     /gene="nhaC"
                     /locus_tag="ACGVJG_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFJ6556013.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(22107..23528)
                     /gene="tyrP"
                     /locus_tag="ACGVJG_000020"
     CDS             complement(22107..23528)
                     /gene="tyrP"
                     /locus_tag="ACGVJG_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFJ6556014.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(23771..25648)
                     /gene="tdc"
                     /locus_tag="ACGVJG_000021"
     CDS             complement(23771..25648)
                     /gene="tdc"
                     /locus_tag="ACGVJG_000021"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFJ6556015.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(25939..27195)
                     /gene="tyrS"
                     /locus_tag="ACGVJG_000022"
     CDS             complement(25939..27195)
                     /gene="tyrS"
                     /locus_tag="ACGVJG_000022"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFJ6556016.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(27307..27512)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            27775..29076
                     /locus_tag="ACGVJG_000023"
     CDS             27775..29076
                     /locus_tag="ACGVJG_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFJ6556017.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(29145..29744)
                     /locus_tag="ACGVJG_000024"
     CDS             complement(29145..29744)
                     /locus_tag="ACGVJG_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6556018.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            29998..30879
                     /locus_tag="ACGVJG_000025"
     CDS             29998..30879
                     /locus_tag="ACGVJG_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFJ6556019.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            30892..32502
                     /locus_tag="ACGVJG_000026"
     CDS             30892..32502
                     /locus_tag="ACGVJG_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6556020.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(32579..33238)
                     /locus_tag="ACGVJG_000027"
     CDS             complement(32579..33238)
                     /locus_tag="ACGVJG_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFJ6556021.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(33521..34186)
                     /locus_tag="ACGVJG_000028"
     CDS             complement(33521..34186)
                     /locus_tag="ACGVJG_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFJ6556022.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(34333..34938)
                     /gene="dhaL"
                     /locus_tag="ACGVJG_000029"
     CDS             complement(34333..34938)
                     /gene="dhaL"
                     /locus_tag="ACGVJG_000029"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFJ6556023.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(34966..35976)
                     /gene="dhaK"
                     /locus_tag="ACGVJG_000030"
     CDS             complement(34966..35976)
                     /gene="dhaK"
                     /locus_tag="ACGVJG_000030"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFJ6556024.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(35951..36346)
                     /gene="dhaM"
                     /locus_tag="ACGVJG_000031"
     CDS             complement(35951..36346)
                     /gene="dhaM"
                     /locus_tag="ACGVJG_000031"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFJ6556025.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            36500..36820
                     /locus_tag="ACGVJG_000032"
     CDS             36500..36820
                     /locus_tag="ACGVJG_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFJ6556026.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(36932..37789)
                     /locus_tag="ACGVJG_000033"
     CDS             complement(36932..37789)
                     /locus_tag="ACGVJG_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFJ6556027.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(37897..39201)
                     /locus_tag="ACGVJG_000034"
     CDS             complement(37897..39201)
                     /locus_tag="ACGVJG_000034"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFJ6556028.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(39198..39707)
                     /locus_tag="ACGVJG_000035"
     CDS             complement(39198..39707)
                     /locus_tag="ACGVJG_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556029.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(39882..42017)
                     /locus_tag="ACGVJG_000036"
     CDS             complement(39882..42017)
                     /locus_tag="ACGVJG_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFJ6556030.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(42100..43755)
                     /locus_tag="ACGVJG_000037"
     CDS             complement(42100..43755)
                     /locus_tag="ACGVJG_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFJ6556031.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(43757..44071)
                     /locus_tag="ACGVJG_000038"
     CDS             complement(43757..44071)
                     /locus_tag="ACGVJG_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFJ6556032.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            44241..44501
                     /locus_tag="ACGVJG_000039"
     CDS             44241..44501
                     /locus_tag="ACGVJG_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFJ6556033.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            44515..44829
                     /locus_tag="ACGVJG_000040"
     CDS             44515..44829
                     /locus_tag="ACGVJG_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFJ6556034.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(45559..46113)
                     /locus_tag="ACGVJG_000041"
     CDS             complement(45559..46113)
                     /locus_tag="ACGVJG_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFJ6556035.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(46239..48168)
                     /locus_tag="ACGVJG_000042"
                     /pseudo
     CDS             complement(46239..48168)
                     /locus_tag="ACGVJG_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(48474..49190)
                     /locus_tag="ACGVJG_000043"
     CDS             complement(48474..49190)
                     /locus_tag="ACGVJG_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFJ6556036.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(49191..51014)
                     /gene="glpO"
                     /locus_tag="ACGVJG_000044"
     CDS             complement(49191..51014)
                     /gene="glpO"
                     /locus_tag="ACGVJG_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFJ6556037.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(51028..52524)
                     /gene="glpK"
                     /locus_tag="ACGVJG_000045"
     CDS             complement(51028..52524)
                     /gene="glpK"
                     /locus_tag="ACGVJG_000045"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFJ6556038.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(52660..54120)
                     /locus_tag="ACGVJG_000046"
     CDS             complement(52660..54120)
                     /locus_tag="ACGVJG_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556039.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(54110..55405)
                     /locus_tag="ACGVJG_000047"
     CDS             complement(54110..55405)
                     /locus_tag="ACGVJG_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFJ6556040.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(55737..56414)
                     /locus_tag="ACGVJG_000048"
     CDS             complement(55737..56414)
                     /locus_tag="ACGVJG_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFJ6556041.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(56444..57535)
                     /locus_tag="ACGVJG_000049"
     CDS             complement(56444..57535)
                     /locus_tag="ACGVJG_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFJ6556042.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(57636..58070)
                     /locus_tag="ACGVJG_000050"
     CDS             complement(57636..58070)
                     /locus_tag="ACGVJG_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFJ6556043.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(58654..60111)
                     /locus_tag="ACGVJG_000051"
     CDS             complement(58654..60111)
                     /locus_tag="ACGVJG_000051"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFJ6556044.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(60108..62003)
                     /locus_tag="ACGVJG_000052"
     CDS             complement(60108..62003)
                     /locus_tag="ACGVJG_000052"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFJ6556045.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(62061..63836)
                     /locus_tag="ACGVJG_000053"
     CDS             complement(62061..63836)
                     /locus_tag="ACGVJG_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFJ6556046.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(63886..65382)
                     /locus_tag="ACGVJG_000054"
     CDS             complement(63886..65382)
                     /locus_tag="ACGVJG_000054"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFJ6556047.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(65576..67198)
                     /locus_tag="ACGVJG_000055"
     CDS             complement(65576..67198)
                     /locus_tag="ACGVJG_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFJ6556048.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(67526..68839)
                     /locus_tag="ACGVJG_000056"
     CDS             complement(67526..68839)
                     /locus_tag="ACGVJG_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFJ6556049.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(69084..69308)
                     /locus_tag="ACGVJG_000057"
     CDS             complement(69084..69308)
                     /locus_tag="ACGVJG_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556050.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(69328..69750)
                     /locus_tag="ACGVJG_000058"
     CDS             complement(69328..69750)
                     /locus_tag="ACGVJG_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFJ6556051.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(69892..70602)
                     /locus_tag="ACGVJG_000059"
     CDS             complement(69892..70602)
                     /locus_tag="ACGVJG_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFJ6556052.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(70620..71117)
                     /locus_tag="ACGVJG_000060"
     CDS             complement(70620..71117)
                     /locus_tag="ACGVJG_000060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6556053.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(71154..71606)
                     /locus_tag="ACGVJG_000061"
     CDS             complement(71154..71606)
                     /locus_tag="ACGVJG_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFJ6556054.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(71741..72346)
                     /locus_tag="ACGVJG_000062"
     CDS             complement(71741..72346)
                     /locus_tag="ACGVJG_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFJ6556055.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(72796..73416)
                     /locus_tag="ACGVJG_000063"
     CDS             complement(72796..73416)
                     /locus_tag="ACGVJG_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6556056.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(73418..74287)
                     /locus_tag="ACGVJG_000064"
     CDS             complement(73418..74287)
                     /locus_tag="ACGVJG_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFJ6556057.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(74310..74717)
                     /locus_tag="ACGVJG_000065"
     CDS             complement(74310..74717)
                     /locus_tag="ACGVJG_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFJ6556058.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(74736..76025)
                     /locus_tag="ACGVJG_000066"
     CDS             complement(74736..76025)
                     /locus_tag="ACGVJG_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFJ6556059.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(76052..76354)
                     /locus_tag="ACGVJG_000067"
     CDS             complement(76052..76354)
                     /locus_tag="ACGVJG_000067"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFJ6556060.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(76379..78472)
                     /locus_tag="ACGVJG_000068"
     CDS             complement(76379..78472)
                     /locus_tag="ACGVJG_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFJ6556061.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(78961..79725)
                     /locus_tag="ACGVJG_000069"
     CDS             complement(78961..79725)
                     /locus_tag="ACGVJG_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFJ6556062.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(79836..80705)
                     /gene="mdcB"
                     /locus_tag="ACGVJG_000070"
     CDS             complement(79836..80705)
                     /gene="mdcB"
                     /locus_tag="ACGVJG_000070"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFJ6556063.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(80730..81806)
                     /locus_tag="ACGVJG_000071"
     CDS             complement(80730..81806)
                     /locus_tag="ACGVJG_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFJ6556064.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(81830..82423)
                     /locus_tag="ACGVJG_000072"
     CDS             complement(81830..82423)
                     /locus_tag="ACGVJG_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFJ6556065.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(82427..84103)
                     /gene="mdcD"
                     /locus_tag="ACGVJG_000073"
     CDS             complement(82427..84103)
                     /gene="mdcD"
                     /locus_tag="ACGVJG_000073"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFJ6556066.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(84093..84392)
                     /locus_tag="ACGVJG_000074"
     CDS             complement(84093..84392)
                     /locus_tag="ACGVJG_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFJ6556067.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(84494..>85558)
                     /locus_tag="ACGVJG_000075"
     CDS             complement(84494..>85558)
                     /locus_tag="ACGVJG_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFJ6556068.1"
                     /translation="PRVDIPGDWVDVVVKADKPYQLEALFTRDPQNITELQILMAMMA
                     IKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLKGKIAKNWVLNPHPTMIPAIE
                     SGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSNRTLSQAAGQYAIDMFIGSTL
                     QLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPAWQSLRDNDDPLGKGQKLVVQ
                     MVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSEDTTHIVTEEGIAYLYKAKTK
                     EERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPEDLGIKRSEAKRSLLAAQTID
                     DLIEWSDGLYEPPMKFRTWS"
BASE COUNT        24744 a        18014 c        14990 g        27810 t
ORIGIN      
        1 aaggtttccc cggcacacgt gtgaacatcg gaacaatggc tagtcgtaaa cgtgtaggtg
       61 aaattttgca ccatgattat cgaacacttg gtcgtttctt gaaagacgct gtagaaaatc
      121 caatcaaacc tgtgaaagta gacaaagcag acgctccagc acaagaagtc gttcatctag
      181 ctaccgatgc agacttcgat attcgcaaca ttttgcctgc acctacaaat actcctgaag
      241 atgctggtcc atacatcaca atgggtgtag tcttaggatc agatccagaa aaaacaatga
      301 gcgatgtaac gatccatcgt atggttctag aagataaaga tacgatcggt atgtacatca
      361 tgcctggcgg acgccacatt ggtcacttcc aaaaacaatt cgaagccttg aataaaccaa
      421 tgcctatcac tatcaatatt ggtttagatc cagcaattgc cattggcacg actttcgaac
      481 cacctaccac tccacttggc tacaatgaac tatggtgtgc aggagctttg cgtaacgaac
      541 ctattcaaat cgttgatggt atagcagtcg atgaagtggg tattgctcgt gctgaattca
      601 tcattgaagc agaaatcatg ccaaatgaaa caatgcaaga agatatcaac acgaactcag
      661 gaaaagcaat gccagaattt cctggctata ctggggatgc taatccagct gtacaagtag
      721 tgaaagtcaa agcagttact caccgaaaag acaatccaat catgcaaaca acgattggtc
      781 ccagtgaaga acacgtatct atggccggta ttcctacaga agcaagtatt cttgccttgg
      841 tcgataaagc tattccagga aaagtagtaa atgtctacaa tcctcctgca ggtggtggta
      901 aattgatgac catcatgcaa atccgtaaag aaaacgaagc ggatgaaggt attcagcgtc
      961 aagcagctat tcttgcttta tcagccttca aagaattgaa gacagtcatt ttagtcgatg
     1021 aagatgttga tattttcgac atgaacgatg tagtttggac tttcaacact cgtttccaag
     1081 gagataaaga tatcatcgtg atcccaggaa tgaggaacca tccattggac ccatcagaat
     1141 taccagaata taatcctggc tctattcgtt tccgtgggat aagtgcaaaa actatctttg
     1201 acggtacagt tcctttcgat atgaaagaac attttgtacg tgcaaaattc caagaagtag
     1261 aagattggga aaaatatttg aaataatgaa tgatctgcaa ctgttttgac atcataaaaa
     1321 ggagtgcgga aaatggaaca attggcacaa cggaagaaaa aaatcgtgat tggtgtaagt
     1381 ggcgcttcag gcaccattta tgccatcaac ctagtgaaga aactaaaaga atatccgatg
     1441 atagaaacac acggaatcat cagtccatgg gcaaaacaga acttaaagct ggaatccgac
     1501 ttatcactaa ctgaattcga aagcctgttt gattaccatt attcaaacaa agatttaggt
     1561 gctgctgtcg ccagcggatc atttttgacc gatggaatga tcatcgtacc ggcaagtatg
     1621 aaaacagtcg caggtatctc tgttggttta ggcgacaacc tgattgcacg tgctgctgat
     1681 gtaacaatga aggagcaacg aaagttagtc attgttcctc gtgaaacgcc attaaatgcc
     1741 attcacttgg aaaacttgac taaattagca cgactaggtg tgcaaatcat accaccaatt
     1801 cctgcttttt ataatcagcc tcaaacgatc caagatcttg ttaatcacca tacaatgaaa
     1861 cttttggatc aatttgaaat tgtaaatgag cattcaaaac gttggcaggg tgattaatat
     1921 ggaaaaagaa acgatagttg aagtaaccaa actaggtttc acgatccaag ctgctgttaa
     1981 agtaatcggt aaagacttat tgatcacttt aacaggcgga gatacaccac atatcggaac
     2041 agtcactaca ttttctcgtg atacagagat gcaaactgta cgtttcccta gccacgatgg
     2101 tcggttccat aaagacgatg tcttagccga aaaaatagct gtgatcatca aaactgccgt
     2161 tccgggaagt tgtacgatca cttccggtat ccatgttgac catatctcca atgcacaaat
     2221 caacgcctct tttccgatgt cagaagagtt aggttacaag attttagatt ggttggaaaa
     2281 aatagatttc catatcaaag aaccagttta ttataaagat ggtgaaaaac cattatgaaa
     2341 gaagaggttg tgaaatcagc gttatgacct gagtatgaga gaaaaaaatg gaaaaatagc
     2401 ttctcatatt ttgccgtttt tgagctaata ccgagggaca gctgattgaa cgccgtttat
     2461 caagaggttg tgataaagcc gcttcgtttc gagtagtaag aaaaattttg taaacatggg
     2521 tttccacatt tttgcataaa tttgacttac taccgagaaa ctggcttttg aacaccgttt
     2581 atcaagaggt tgtgacacgt cttttgtcac aacctctttt atttttgctt cactgtaact
     2641 gctacaatat caccgatttc ttttccgatg tttttacgaa tatcttttcg tagtccaagg
     2701 atgtagcaga ctgaaccatc tggatttttc acgcccatat tcacgatact gccttcatac
     2761 ggcacattgt caaaagttgc ttggacttta actcttccag caccgaattc ttttcttata
     2821 tcatacggaa aaataatata tgccccgccc ttgccgactt cggaagcata gatttttgat
     2881 tcaaaagaat aaatttccct cattttattt ctcctatttt ttacatctag tatagcatag
     2941 caaaaaaaag aaggaactgt gacaaaagtc ttgtcgcagt tccttttttc gaataaacgg
     3001 tgttctcctg ccaactcctc ggtcacaagt cacaatagtc aacaaacatg agaagctgtt
     3061 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct cttccttaaa
     3121 cggtgttcaa aagcttgcat ctacggcact aagcccaatc agatgaaaaa tatggagtgc
     3181 tatttttatc tgattgttct tactgcttga tgcagagcag ctttttcaca acctcctaca
     3241 tggtgttcaa aatattattc gccttttgct tctttcacta attttgcgaa tgcttgttgt
     3301 tggaattcag cagctaaggc tttcgtacgt tgttcttgct ctgctttgct catttttgga
     3361 ttttcagtca ttgcttttac cataccgtct tgaacataag cttttgcttc actttccatc
     3421 gtactttgct gtttcttcac taattcagct aacttggctg cttggtccgt acttaattct
     3481 acccatgtct ctgggatttt gaatgtattc actcggctgt catattcgtg attgttatca
     3541 gcaatattga accagaattt atacatgtcg tttttgtatt cccaataagt agttttcgtc
     3601 actttttcag catcgccgtc tgtcttttct actttatttg tttcgttatc cgtaccagtt
     3661 gcttttggtt tttcttgttt ttcatttgtt cggtaaagat acactttttc ttttccattc
     3721 cctaaagctt tatacaaaag catatctgct ccttgggaat cagcactgga aacaattttt
     3781 tctgtttttg tttccgttac tttttccatt ccaaagtgat tggacaaatt agcgacgatc
     3841 aaacttagtg aagcgacgaa gattagtccg aaaatcaaag ataaaaaggt ttgccatgtt
     3901 tttttagcga acacgttcgt taaagcaaaa gcaagcaagc tgacaatcaa aactccaata
     3961 atcatgcatt atcccccgct ttcattagaa caggttcttt tttcttcaat aaaaaggcaa
     4021 taataaagcc aagaatccca aagatgatcg ctacgataaa cgcggcatgg taacctgaca
     4081 gtgtggcatt gattgcctga tctttataag ctaatggcgt attatccaat acagatttgc
     4141 ttgggatatt tgcttttgtc acattcgtca acacactgat caaaatagct gtaccaatcg
     4201 agcttgcgat ttgacggaat gtattattta ccgctgtccc atgactgatc aagttatttg
     4261 gcaatgcatt cataccagaa gtcgttaccg gcatcatgac catggagatc ccaaacatac
     4321 ggatcgcata aaggatcacg atatataaaa ctggagtcgt ttttgtcaag aaagcaaatg
     4381 gtgcagttgc cgctgtcaaa atgaacatcc ctgtgatggc taaacgtttc gcgccgtatt
     4441 tatcgaaaat cgcacccgtc acaggcatca tagcccccat gatcaatgca cccggcaaca
     4501 acatcaagcc agaatggaat gctgattctc cacgaatatt ttgaatatac aatggtacga
     4561 ccatttctgc ccctaccatc gccatatttg ttacaccagc taaaacagct gcaatcgtga
     4621 atgtagatga tttgaataca cgaagttcca agaatggatg ttccaaatgt aattgacgcc
     4681 atgagaacaa ggcgatgaaa atcacaccaa caactaagaa accaattact ttcaagcttc
     4741 cccagccgtc atctcctaca ctagaaaatc cgtaaagtaa actaccgaat ccaatcgttg
     4801 ataaaatagc ggataaaata tcaatgctag gatttgacaa tggaataaca cttttcatta
     4861 ggaagaatgc taaaaccaag acaagaacaa cgattggcag tactatacca aacaagtcac
     4921 gccaatggta gttatcaatg atataaccag ataatgtagg gcctaaagca ggagcaagtc
     4981 caataacgat ccctgacatc cccattgctg atcctctttt ttctggcggg aaaatagata
     5041 gcatgatatt ttgcaataat ggcatcgata ctcccacacc gcaagcttgg atcaaacgac
     5101 cgattagtaa tgtactaaaa ttaggtgctg taaagcaagt aatcgttccg atcaaaaaga
     5161 tcgtcatggc agaaagatat aaatttcttg aactgaattt attgatcagc catgctgtta
     5221 ttgggatcat gatcccgttg actagcaaga aaccagtcgt taaccattgc acatcagacg
     5281 ctgaaatatc gaaagctttc atcaatgtag gaaaagctgt cgtcaataag gtctgattca
     5341 aaacggtaca aaatgtacca attaataaga caacaactaa aagtgtccga ttatatggtt
     5401 ttccgtaaat atctacgggt tggttgttac tcatatttct ccttctttct gaattttttc
     5461 tgaaactact agaatacttt agtcctaaat tcgctatttg tcaacctact tgacacataa
     5521 gttaatacgg atataattaa gggaagcaag aggacaaaag gagtgacaaa aatggttggg
     5581 gcttccctta aacgtttgtt ggaaaatgat caattggttg ttggcatcag tgaattaagt
     5641 gatatcgtag gtgtgtctcc acgtcaatta aggtattggg aacaaaaggg attcattcgt
     5701 tcgatcgaag ctgatgcgaa ttgtcctaga aaatatcgtt tacctacagt cataaaagta
     5761 gaactgatca aaaaatattt agatgacgga tttactttgt caaaagctgc agaaaaggca
     5821 gaaatccgcc aaaagaaaat gcaccatgtc cgtaaagttt tttcaaaagc aatcaaagat
     5881 atcgaattga tcgacgaacg ctatacgatt ttacaaatcg gtaacttcaa agaagagaat
     5941 gctaagatgt acatcattca tgacaacgag acagacgaat taacttacaa gctccttcct
     6001 gtcgatgaaa aagctgacta cgaacagttg ttaaaagaac tctaaacgcc actaatctcc
     6061 taagtaccaa gctttttaat aagtagatgc tacataaata cttgaaaaaa ggcctgtttg
     6121 aacataagaa tatccgaacg gtgatctgcg tttctttcat atagaaagga attctccatc
     6181 actgttcgga tattttttta tttttttcgg ttactttctt ctaatacatc tgttagccaa
     6241 tttgcatatt ctgtttcccg attgatcgca tgcgctaaaa tcaggtagtg tccgtaattt
     6301 tcatgttttt ctttttcagt aggaaagatc gttttttttc gctctaatag atgaagtcgt
     6361 ttttcttcat gaaggtcacg ctgctgctga ataatttcta ctaatgccgg atcattcacg
     6421 tctttgacga aatacaattt cagaacaaat tcgtctcgat taaccggtag ttcattggtg
     6481 ggtgtatgaa tccactctgc caattgttcc ttcccttctt ctgttaattc ataaactttc
     6541 ttttccaatt tttcacctgt gatctctact tgatgctgaa tgattccttg ctcttccatt
     6601 tttgcaagtt caggataaat ctgactatgt tttgcttgcc aaaattctcc tatttcattt
     6661 ttgaacgatt gattcaattc atatcctgtc atcttcttct tacttaataa ccctaacaaa
     6721 atatatcgaa gtgtattttt tcttgccatc agttttccct ccttgacaaa tctttaaatc
     6781 cattctatca taaatgagta atcgaaaaca tgtagtttat tacatgtttt aatttataag
     6841 gagatgctta aacatgaaag aatttaaaga attggaagat tttattggaa ctcactttat
     6901 ctatacgtat gacaatggtt gggaatatga atggtatgcc aaaaatgacc atactgttga
     6961 ttaccgtatc catggcggaa tggtcaaagg ccgttgggta aaagaccaag aagctgatat
     7021 cgttaaattg acggacggcg tattcaaaat cacttggaca gagccaacag ggacagacgt
     7081 ggcattggac tttatgccaa atgaaaagaa acttcacgga atgatctttt tccctaaatg
     7141 ggttgaagaa catccagaaa tcactgttac tttccaaaac gaacatatcg cagaaatgga
     7201 agcagctcgt gaaaaatatg aaacttatcc aaaattactt gttcctgaat ttgctacgat
     7261 cacttatatg ggtgatgcag gtatagacaa tgaagatgtt attagcgaag aaccatacga
     7321 tggaatgcca gatgatatcc gtagcggaaa atacttcgat gaaaattata aacgagtaaa
     7381 ataaggttgt gaaagagagg ttgtgaaatc agcgttctga cctgagtatt agagaaagaa
     7441 acggaaaaat agttcctcat gttttgccgt tttttgagct aataccgagg gtcagctgat
     7501 tgaacaccgt ttatcaagag gttgtgaaat cagcgttatg ctttgagtaa taagaacgct
     7561 agttagaaaa tagcttttca tattttctag gctagtgggc ttattaccga gaagcagccg
     7621 tttgaacacc gtttatcaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa
     7681 caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg agggtcagct
     7741 gattgaacac cgtttatcaa agattgtaac aactgcatat tcacacagtt aaattagtga
     7801 cttcaaaatg aaataatgct cccagcaaaa aattaggctg ggagcattat ttcgttattc
     7861 tgttcatcaa aaatcagcgt tttgattcca tttcatcaca agttcctcct catttactgc
     7921 ttcatctttt tgctgtgttt gaattgtaaa accttgtgaa agatagaaat gaatagctga
     7981 ttgatttttt tgatagactg tcaaagtcag tgttttattg gtttctttta tttttttcat
     8041 tagttgcgta ccgattcttt gttgccggta ttcccttttc acaaagattc ctgcgataaa
     8101 ctgatcaatc accccagcaa aaccaataat ttcttgatct atttctgcta agtaaatcac
     8161 tgcatctggt aaagctttcc taacagattc tacttgattc tcccaataag ttgatggaat
     8221 gaattcatga gaatccaagt tagcatgcag ccaaatcgat agaacagatt ccagttcttt
     8281 ggcagacaat tttgttgttg tgcggatcat tttttctctc cttacttatt cattgatttt
     8341 aaaataaaaa ccattttttt gaagctcttt tacaagtttt cgcccctcaa aaaagtatat
     8401 cttctcttgt tttacttttc taataccaaa agaaacagca actttaggat catcagctga
     8461 aacgattcga taagttccac caggcaattg atccagcttg actttcccat gatggtcgct
     8521 ttgtactgaa aacggcacac catttctagt aagtgttttt ttcttatgtt tcaaaatgaa
     8581 tgaactacca tttaccggtt tccctgacga atcactaata ataaatgaaa aatcaagcac
     8641 ttttttactt aatctatact gatagattag tcgtccgata aacactaaca gtatgactac
     8701 agctattatc gttcctgcaa gaataaacca ttgccgcatg gttctttcct gatttccttt
     8761 tccttcttct ttttttaccg attcgttata aggaatccga tgtcctgtca ctagcaaacg
     8821 atgcgtgttg atcatatacg gcgtacatgt aagcaaggta actaagtctt ctccttctga
     8881 aatcgttaga acagacgtgt catctggcag taccacttgt atatttttaa cttgataagc
     8941 caatttctcc cccaaaacag tcaaaataaa cgtgtctccg ttttctaaac gatctaattc
     9001 ggtaaataac cgtcgattag gtaaaccgct gtgagcagag atcactgaat gcgtcgaatt
     9061 tccaccaagc gggaaagagg ttccttgcaa gacgcctgct ccattttcaa ggatcctacc
     9121 attgagccga tcataaagtg aaatatttaa ttggatctta ggtatattga tacttccgat
     9181 cagccattcc tctaattggc tggatgttgt actttcattt ctgttctttt cattgaaagg
     9241 atcagtacca ggattgaatc cttggtcagt taatcgttgg tttttctcct ccattttctt
     9301 tcgctgcact tcaatttcag ctgcggtccg tttttgtact tgttcgatac gtttttgatc
     9361 aataaaacta tttaaagaat ccacataaaa tggatataaa gcgatcagta accctacgac
     9421 gatacatatt aataaaaaca aacgaaacaa aagtcgacga gactgttttt gcttactctg
     9481 cttcacatgt gtcctccctt gcaagttttt ttgttctttc ttttattata tgggttcagg
     9541 aaaaaaatag aaaatgaaag ccgatgaggt tgtgaaaaag ctgtcctgca tcaagtaata
     9601 agtacagccc aaaaaaatag ctactcatat tttttgttgg gttgggctta ttaccgcaga
     9661 tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa actgcgttat gctttgagta
     9721 ataagagcgc tagtcagaaa atagcttctc atattttcta ggctagtgag cttattaccg
     9781 agaagcagct gattgaacac cgtttaccga gaggtcatga aaaagctact ttgtttcgag
     9841 tattaagaaa aattctgttt caaaacaagg agatgcaaca aaacttatgt cgcatcctct
     9901 ctccttagaa ctttcactta tgtttttctt gtaaaattca taaatatttt catgaatttt
     9961 ctaatttctt gcctacctct cgcataggat atatgatgaa tttatctaaa gtttgcaaag
    10021 gagttcgaca attgaaaaaa tatggcccat ttttagcaat ttttttcatt gctttggcat
    10081 ttctcatgcc tcatttattc acacataaac taattttggg tgctgatgcc ccattccatt
    10141 acaatcggtt ctatgatgcc gcgcaacaaa ttaaatatgg ggatttccat tattttcctt
    10201 ccctttatgg attccagcaa agtggcagga tcgtgaatgc cgtttacggc ccatttttcg
    10261 cttatttaca aggattgatc gtgttgcttg caggtagctg gtttcattat cagattcttt
    10321 caaatttcat tctttatctt atttcaggat tttctatgct gattttactg agatatgtca
    10381 atgttcgaaa ttgggtaagt gtaccagtag ccgttttatt catgagtacg tattctattc
    10441 aatactggtc tatcaaccaa ggattcacta gctgggggac ttgtttttat cctttatgta
    10501 tgatccctat tgctgatttc gtccttcata aaaagttccc tgttatcaaa gtcgctgtct
    10561 cagtgggact tatgacacag atccatttac tgtcttcgat catgttagct ttgatgtata
    10621 tcccttttta tctttattac ttttttaacc agactgaaaa aggaaaagca ctgcttcaac
    10681 tgattaagtc ggtcttgctt tattttgttc tgacagcaaa tatttgggca ggattagcta
    10741 ttgtttacag tgggaataag attcttcctc cctttgttaa cagagaaatg agtcaaaagg
    10801 cgatagatct ggatggcagt tattggctcc attaccctaa ggcctttcct atattgatat
    10861 tatcaggttt tttgcttttt attttctttc aacaaaagaa caccttattc caaaaaatcg
    10921 tgttgtacac aagtcttgtt ttcttttttc tttctactag cttgattcca tggacgacat
    10981 tgatcgaaaa aaatgtgcct tttattagtt tgatccagtt tccatttcga tttttcgtgc
    11041 catttacgct attgcttcta ctttaccttg ccctttcact gaaccagtgg tctgaacaaa
    11101 agtggttccg tcttttaagt atatttggca ttcttatttt ttcagcacag acgatccaga
    11161 acttatacca gcatctggaa aagtgggaaa atgaaacatt tgtcagtcga catacttatc
    11221 tattcgatac gccggaagaa gcaaggaaaa cattcttcag caaagatatg tctaccagct
    11281 tgttcgtttt ccaaaaaacc acacctgact atctgccaat ctataaggag acaaaagaca
    11341 ataaatacga ccggtataac gaagaaatac tagaaaacga aacaactttt ataaaaacac
    11401 atccaggcgg tcttttgaca atcaagtgga aaaacactgg ggacaaaact gttcatatcc
    11461 ctgttatcgt ctacgatcgg actgtgctac agcaaaatgg aaaaacattg actgattatg
    11521 aagtaacaga tatcgggacc ccaatcgtga agcagcaaaa aggaatcaac gagctcacgc
    11581 ttcattatca aacgccaatc tatttctatt tcattctttc tcttactttg attggctggt
    11641 ttaccttgct atgtttattt atttatcata gatacaagct tttacgtgct taggcaaaag
    11701 ggataaatac atccgatcaa aaaactctcc aaacggtata atcgtttgga gagttttatt
    11761 ttaatcagtt tttttatgtt atatgttata ttttttattc gttcacatct aattcgattt
    11821 ccaccccgta gtgatcactg atgattggac tatcgatccc atcaaaaatc cgttgatatt
    11881 ttttaacctg cattccttca gggacgaaaa taaaatctat acgcaatttt tctgtatttt
    11941 gttcccaacc atctattttc ttttccacag tagctgcttt tcctttttca ttcgcgacaa
    12001 catacgaatc ttgaagaaga aggcaacttt ctcttacagt ataataacct tgagagtcta
    12061 ctggattatt gaaatcacct aggaaaaaaa gtggtacgcg agtttccaaa aagtagtttt
    12121 ccaagttttt ccactcaaaa taaaagccac tttttttatc tgtccaccaa gaaaagtggc
    12181 agctcgagac aaaaatgtcc tgatcatcta tctttgtttt cccaatcaag atcttgcgtg
    12241 tctgataatc ttctttcttt tgaaattggg agacggtaag gacctgactt tccaatgttt
    12301 ttttcgtaag cagtgcattt ccttcttcat atttatcata accgatgtgg ctcatttccc
    12361 aactccaaaa atactctttt ccgtgttcct ttaaaagctg aactaaacgg taggcaaaat
    12421 tatctttacg gataggtact tgattcttta ccggacagaa cttcattagc ttcgcaggtt
    12481 ctacttcttc agattccagt aattggttga tttcttgtaa agcaatcact tcataatctt
    12541 ctaacaggat cttatctgct aattcttgca attttttctc tggttctggc tcaagccagc
    12601 tatgcgtgtt gagtgtcatg aatttcattg gttttctcct tgtcttcatt tcgaaacaaa
    12661 agggttgtga caaaattttt gtcacaacct ctccagcaaa tagtgttctc ctgctaactc
    12721 ctcagtaata agctcaatgg accaggagct attttttgtt tggctgtttt tatcacttgg
    12781 tgcagggcag ctttttcacg acctctccac taaacagtat tcaaactgct gcttctcggt
    12841 aataagccca accaaacgaa aaataggagg agctattttt gtttggctgt tcttatgact
    12901 tgatgcagga cagctttttc acaacctctc cttacatttt aatggagccg atcactttac
    12961 cagctgtcac tgtacctaat tgcttgatca ctacttcttc tactttatcg tttgtaaata
    13021 cgatcatgat cgttggatct ttttcttcgt tttggatcac atcaagatcc atctcagcta
    13081 atttcgttcc tgttcctact tcttggcctt ctttgacagc aatctcaaaa gctggctgat
    13141 tcatttggac tgtatcgatt cccatatgga tcaaaacttc gataccatct ttcgtttcca
    13201 gcccaattgc atgtttgcta gggaaaatac ttgtcacttt tccagcgatc ggcgcaacaa
    13261 cttctcttga tgttggaata acagcaaagc cgtcacccat cattttttgt gagaataccg
    13321 gatcttttac ttcagataaa ggaatcactt ctccagaagc tgcggaaact agttcttgtt
    13381 ctatacctaa gtattgctta ctacctgctt gtccatttcc tgtagaaacc atttctggct
    13441 ctggaatctc tgctcctgat tgcagcagat cttcgatatc tgatttcaag acatctgctt
    13501 ttggtccata aactgcttgg actccattgt ctttgacgat caatcccatc gcccctgctc
    13561 gtttccaggc ttcttcagtc cctacttttt ctcgatcatt tacacttacg cgtaaccgag
    13621 tcatacaagc atcgacatct ttgatatttt cttttcctcc cagcaaatga atgatctgga
    13681 ccacttgctg atctgctgtt cctgttccgc tgttagatac aacggtttct tcactgtcat
    13741 tgtcgtaatt tccgttacgt cccggtgtag caaagttgaa ttttttgatc aagaaatttg
    13801 ctaagaaata cgtaacaaca ccaaaagcga tcacacacaa cacgaagttg ataagatctc
    13861 cacctagacc cgctttgata gctaacggtg tccgagtcag caattcgata ttcccaaatg
    13921 aatggacccg caatggcaag atatctgcca tcgcaaatgc agccccttgg atcactgaat
    13981 aaatgacata taatggcaca gcagcaaaca taaacatgaa ctccaatggt tctgttacac
    14041 ctgttagaaa gacagccaac gctgctgaaa aatacattga tttatatctt gctttcttat
    14101 caggatcgac attacgatac atcgcaaaag ccattcccat caagatacct gaagaaccaa
    14161 tcatttgtcc tactttgaaa cgagccggcg tccagttatg caacacaaac tcatattgcg
    14221 acatatcccc tgcaccttta agattcacta aatcagtggc ccaagccaac cataacggat
    14281 cttgcccaaa tacttgtgtc cccgcctgtg cgcctgataa aatctcatac gatccgccaa
    14341 gctgtgtata gttgatagga atcgtcagca tatgatgcaa tccaaatggc aatagtaaac
    14401 gttctaatgt tccaaataaa aacggtgcta gaatcggtgc gctttcttgt gattctgcaa
    14461 tccataaacc aaaattattg attccagcct gtacgtttgg ccacaccaca gcaagaatca
    14521 atgccacgat tgttgaccat aagataacaa caaacggaac aaagcgcttt ccattgaaaa
    14581 atgacaaagc atccggcaat tttctataat tatagtactt attataagcc attgccccga
    14641 caaatccagc gatgatccct acgaatactc ccatattcaa agctggtgct tccaatacac
    14701 ttgtaaaaaa tcctttaacc atgatcttcg taccgaacag agtgtgggta aatgcatctt
    14761 cattcgcaag catgtcactt gtgacaccaa agatagcccc agtgatccga ttgattaaaa
    14821 taaatgagat cccagctgca aatgctccgc ctgctctttc ttttgcccaa cttcctccta
    14881 ttgctaaagc aaataaaata tgaagattac caataattgc ccaaccaata ttttcaatta
    14941 ctccgcccgt tgtgacaagc ggcgtccaat caggattgat caagggaatt gatttcccta
    15001 tactgatcat caacccagct gccggcataa ctgctaccac aaccattaaa gcctttccaa
    15061 atttttgcca aaactcaaaa ctaaatatct ttttcattcc gtttagctcc tttacgcaat
    15121 cggtttcgtt aaacgtatta tagcgtgcgt tttctttttt gtaaatgctt tattttttat
    15181 ttttctgtct ataccattct ataaaaccta taatacgtgt gttcccttct ataattttct
    15241 cctaaaaatt tattttttta agcttttttc tatgaaatat tttcttttgt ctattgactt
    15301 gtgcaatcgg ttgcgtttat tatgaacgta taaaacagct cattaggagg agaaaaatga
    15361 aacagatcaa acgactattt gctattgatc catggaaaat cagcacgaca caattagata
    15421 aaaaaaatct tcgactgcaa gaatctttaa caagtattgg aaacggttat atggggatgc
    15481 gcgggaactt tgaagaaact tactcagggg accaccatca aggtacttat ttggcgggtg
    15541 tctggtatcc cgataagaca cgtgtcggct ggtggaaaaa tggttatcca gaatactttg
    15601 gaaaagtgat caacgccttg aactttatcg cgatagatat ttcgatcaac gatcagctga
    15661 tcgacttggc ttctttggaa ccagaagact tttactggga attagatatg aaaaatggtg
    15721 tattatctcg tacattcaca atcactactt ctactaataa agtacgcatt tccttcgaac
    15781 gttttctgag cattgtcaaa aaagaatcag cttatatcta tttaactata gaaatgcttg
    15841 aaggtagcgg aacagtcaaa ctcgtatcaa aattggatgg aaatgttcaa aacgaagaca
    15901 gcaactatga tgaacatttt tggaatgaga ttgatcgtgg aactatagaa gaaatcggct
    15961 atttgacgac taaaactatt ccaaacaact ttgggattga acaatttact gttactgcag
    16021 caatgcgcca tttcgttaat ggaaaatcag tcatccctga ctatcacgaa gatactttag
    16081 cattacatgg ttgcttgtct gcagaattat ctcaaggtga gacatggtca gtggaaaaag
    16141 aaattatcgt tcttactagt agagatattc cagaagcgca gcaagcagaa aaagctgtct
    16201 cagaattaac gaaccagaca aacaattata tccaagcaaa agaagagcaa acagcggctt
    16261 gggcagaacg atggaagctt gccgatgtgg tcatcgaagg agacgaagaa gcacaacaag
    16321 gtattcgatt caatttgttc caactattct ctacttacta cggcgaagat gaacgattga
    16381 atatcggccc caaaggattc acaggagaaa aatacggcgg agccacttac tgggatacag
    16441 aagcgtatgc tgtccctctt tatcttgctt tagccaaacc tgaagtaact aagaatctgc
    16501 tgaaatatcg gcataatcaa ctgccgcaag caatccacaa cgctcagcaa caaggattaa
    16561 aaggcgcgct ttatcctatg gtcaccttca ctggtgtcga atgccataac gagtgggaaa
    16621 tcacattcga ggagattcac cgaaatggcg cgattgccta tgcaatctac aattatacga
    16681 attacaccgg cgatacggaa tatttgaaaa atgaaggact ggaagtttta gcagaaatcg
    16741 cacgcttctg ggcagaccgt gtccattttt caaaacgagc aaataaatac atgatccacg
    16801 gtgtgaccgg cccaaacgaa tatgagaaca atatcaataa caattggtat acaaatacga
    16861 tcgcagcttg ggtcttgcgt tacacgcgag aaaattattt aacttatcaa aaagaagctg
    16921 ctatcactat cagcgaagaa gaactagcca aatggaaaga tatcgcagag aacatgtact
    16981 tcccagttga cgaagaatta ggaatctttg tccagcatga cacattctta gataaagatc
    17041 tcatgcctgt atccgatatt cccacaagcg atcttcctct gaatcagcat tggtcttggg
    17101 acaaaatcct tagatcttgc ttcatcaagc aggcagatgt actacagggg atttatttct
    17161 tcaatgatac ttttacaaaa gaagaaaagc aaaaaaactt tgatttctat gaaccaatga
    17221 ctgtccatga gtcttccctt tcgccaagta tccattcgat tttagcagca gaattaggaa
    17281 tggaaagcaa agctgtagaa atgtaccagc gaacagctcg cttggatttg gataattaca
    17341 ataacgacac agaagacggc ttgcacatca cttccatgac tggtagctgg ttgtcgattg
    17401 tccaaggatt tgctcaaatg aaaactgccc atgatcagtt gagattcgca ccatttctgc
    17461 caaaaacttg gaaagcttat tctttccata tcaattatcg cggacgttta ttgttcatct
    17521 cagtcacaga tgaacaagtt actttcgagt tattagaagg ttctcctcta gaaatttacg
    17581 tctatgaaca gtcttattct ttagcggatc gtctagaaat aacgctaaga aaggaggaag
    17641 caaatgttta aaggggtatt gtttgattta gacggtgtca ttaccgatac tgctgaattc
    17701 cattatcgtg cttggcgaaa gcttggtcag gaaatcggca ttacgatcga ccacacattc
    17761 aacgaacagc taaaaggcgt cagccgagaa gactcactct ctttgctttt agcttatggc
    17821 ggaaaagaac actctttttc aaaggaagaa tttgcagaat tagcgaaaag aaaaaacgat
    17881 tattatttag agatgatcca gacgatcgaa ccaaaagatg tcttccccgg ggtcgtgcct
    17941 ttactggatt ctttaaaaga agaaaaaatc aagattgctc ttgcttctgc aagtaaaaac
    18001 gggccattcc tacttgaaaa aatgggtctt acaccttact ttgatgctat tgccgacccg
    18061 gcagaagcaa caaacggaaa acctgcgcca gatatcttcc tccttgcagc aaaagctgtc
    18121 ggactaaaag cagaagaatg tatcgggatc gaagacgcgc aagccggtat ccaagccatc
    18181 ctctcaagtg gcgctcaacc aattggcgtg ggacgtgctg aagaccttgg agaggagatc
    18241 ccgcttgtcc ctgatacgac ttttcttacg atcgattact tgaaagaaaa gtggcatgac
    18301 catggataat ccagagatct ccgtcgtacc agttccagag actgaaataa agaaaatcac
    18361 attgaagaac gactacttag aagtcgttct tctcaattac ggtgcccgcc tgcaccaaat
    18421 cttcgcacca gacaaagaag gaaaatcaga aaatgtttta ttgtcttacg atcgtttcga
    18481 agatgttcta acagacaaaa gcttttttgg agcaactgtc ggacctgtag ccggaaggat
    18541 ccgggatgct tcttggggtg agcatagatt ggagaaaaac tgcggatcac accatatcca
    18601 cggtggcacg aaaggctggt ctttccaatt ttgggaagta gaagttttca aaactccatc
    18661 atcaataggt gtggtctttt acttaaaaga tgaattttct acctatccag gaccaatcac
    18721 tgctacgatc acttaccgtt taacaaaaaa cgaattagag atgacaacct ctgctagtag
    18781 ccttgttgaa accatctgca atccgaccaa ccatgcctac ttcaaccttt caggaaatgg
    18841 aacaagagac atctatgaac atcagttaac ggtcttttta gatggtgtgc ttgaattaga
    18901 tcaagaaaaa ctgcctactg gaaattggac aaaaaaagaa aaactgccaa tcgactttag
    18961 aaaatcccca acactgcaag agatccttgc atgttatcca gatggactag atgatgtatt
    19021 tcttttgcat catcctaggc tttccaaaac ttccttacaa ttattcgaga agcattctgg
    19081 tcgccaaatg actatagcaa cttctaataa aagtatggta ttattttcta cgactggatt
    19141 cgaagctgat ttctctgtca atggaaaaca gatgcattca aactacggat tagcaatcga
    19201 accgcaagaa atccctgata tcgttcattt ccccaaattt ggatcgatca acctccatcc
    19261 tggtcaagaa cggatcagtc agacgattta tcggttctct gcacaataac aagagaaagg
    19321 tgtttttatc atggctatta ccgtaaaaga tgtggcgaaa aaagccggtg ttgccacctc
    19381 gaccgtctct cgtgtcatca acgatcatcc tagtatttcc gagacaacaa agaagaaagt
    19441 ccgaaaaatt atggatgaac taggttatgt tcctaacatg actgcccgca atctaggaaa
    19501 acgcatttcc agtgccatcg gcgtaatctt accccctctt gattcaaaag agcggatcgg
    19561 taacccattt tatcttgaga tcatggaagc aatcaacgaa gaagcgcgga attatgatat
    19621 gactacagca atcgctactg ctaaaaattt tgaattgctg ttagagaacg tccagcgtat
    19681 gcatttacaa aaacaagtag atggttttat tctagtgtat tcagatagtc aagatactgt
    19741 cattgattat ttataccaaa accacatccc tttcactttg atcggacagc cttatcatca
    19801 tgagaacgaa atcgtttatg tagataatga taaccagctt ctaggtaaac aagcaacaga
    19861 atttttgatc cagaatggac ataagaatat cctgtttgtg acaaacacga ctcatgaaag
    19921 tttgtatttc gaacgatatt tcggttatca aaaagcaatg atgatggctg gtcttcctgc
    19981 gttatccagt gtagtactag agcatccgga ggattatctg tcctttgaga aaacgttgaa
    20041 agaaaacgaa gccacagcat tggtggtcat tgacgatatc ttcgcccttc gcacgatgca
    20101 gttggcgcaa atgtatggct atgtcattcc agagacactc tccgtcatta gcttcaacaa
    20161 ctcgatcttc tcaacattaa cacaccctta ccttacaagt atcgatatcg atatctccga
    20221 attaggacgt gtcgctatgc aaaagctgcg cgaattgatc catgaagaat tgagcagtgg
    20281 cgtacgtttg gtcatccctc atcgcttgat caaaagagag acagtcgttc ctttagaaaa
    20341 cccatactaa atagctgact cactaaatac gctgaccatt tatcctatgg tcagcttttt
    20401 atgtaaaaga agatactact tctaacacat tgtttatctg taaaaaagag cgtgggacaa
    20461 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa
    20521 ctccttcgga aataagctga aattcacaaa aatttaaaaa gcaattttct tgagtttctt
    20581 cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttccttcct tcaatttttt
    20641 acacgtcgac gattcaataa aaaaccgcct aatattgtaa agacaggtgc aagaatcgag
    20701 aaaaaggcga atggcaagta atgcaacgcg ctaactttca tcgttcccat aatgaatgtg
    20761 ccgctgactc cccacggtac gagagaattg acggtcgcac ctgaatccgc tagtgttcgt
    20821 gtcaaatagg tttttgataa gccaacgtca tcaaaagaac gcttgaatgt ttctcctgga
    20881 agaatgatgg acagatactg ttctccaacg atcagattga ttccgataca gctcaaggca
    20941 gtaaacccga ctaaacgaga aggtcggtcc atcttttctt ttaattcttg aacgatggta
    21001 tcaacgatca gatatttgat cagtaaccca cctaatgcca atgcaagtaa aatcaaacta
    21061 gcagaaccta acatactcat gatcccgcct cgagaaagca gttcatctag ctttggatca
    21121 ggtgtatttg cgacataacc attcatcaag atgtctgctg cttttccgac cgtcaaactt
    21181 ttctcatgga taaaagaaag aacgagagca gctgtcgatc ccgctagtaa tgtagggata
    21241 gccggaattt ttttgattgc ccccccaaat aaaataacta gtggaatcaa tgtccaagga
    21301 gagatccaaa agctcgcttt catgttttgg atcatttcat tgatactagc tagatcggct
    21361 gaatgactgt tatgtcctaa tatcgtataa aaaatcaacg aaaggacgaa tgctgggaaa
    21421 tccgtataac gcataccagc gatatgttcg aatagattaa ctcctccaat acccgctgcc
    21481 aagttggtcg tatctgataa aggcgagcag ttattcccta aaaaggcacc ggaaacgact
    21541 gcgcctgtag tcattgcatc attgaatcca agtaaatgac cgatccccaa aaaagcaatt
    21601 ccaatcgtcg aaatcgttgt aaatgaactg ccgacagtca ctccgacaat tgcacaaaca
    21661 agaaatgctg tcggtaaaaa gaaacgcaca gaaacaagtt taaatccata aaccataatc
    21721 gtagggatgg ttccagataa aatccatgaa gcaactaaga caccaatcaa aagaaaaatc
    21781 aggattggaa tgatccctgg agtgattcct ttgatgatcc cgtcatggat ctcatcccat
    21841 gtgaactttt taaatctgcc ataaaacaac aaacacatga aagctgcaag gatcgggata
    21901 tgtggcgaga gctggaatcc gatgatcagt actcctagga gtgccaacaa cacgatcaat
    21961 acagcgatac tttcttttaa agaaatattt ttattcatcg tcgatctcct atttcaaatg
    22021 aaaaggccct gccatgaaaa aactattcac ttggcaggac ctcctactaa atcagcgaca
    22081 aaacgtcggc tgaaagactt atttttttat ttttcttcta aacttttttc tacctcaatc
    22141 ccccaagaat ctttcttgaa gtgattgatg atcaatggaa taacgaacat gaccactgtg
    22201 atagctgcta ctaataatgc atcactcaat gtcgctgtac ctaatgtagc aaatacgaca
    22261 acgatgatag agaagatcag catgcagctg actacccaag ctaatccgtt tccgttttta
    22321 ccaattcggt aaggacgttc gaattctgga tgtttcttac gcattttaag caatgaaaca
    22381 gcaatcaaaa tgtacacgat acaataaata acagttgtag catttgtaag tgtcaagaat
    22441 acagcactta cgtcatccat tacaccataa agcaaagcaa ataatgaaat aacgattgat
    22501 tgtgttagaa cgacatttct agatacgccg tatttgtttt ctttatggaa tccgaatttt
    22561 ggtggaagga atccttcacg agctacttgg atgattgttt ttgatggtcc agtaacccaa
    22621 gctgataatt gtaaaagcac accaataaat accatgaagc tgaagatatt tccgatcact
    22681 tctggtaagc ctaggatttg acagtcaatc aagataggct gcgtgatatt tgctaattcc
    22741 atttttccat ctggtacgac atttgctaca agcatcgcat tgatgacatt caataaaact
    22801 aatccgatca aagcgataaa tacgcctttt gtataatttt ttgttgcgtc tttcaaacga
    22861 ggcatgtaaa cagaactcat ttcgatccca acaaaaataa atgtgatacc agctaagtat
    22921 ttcaatgaat caatatgttc caagtcaggg aaagcttttg accatgaaaa gtcacctaga
    22981 tagccgtttg agactagtcc gactttgaag gctgccaaaa cacctaaaac gaacatgatg
    23041 actactggga tatatacccc tagccataca ccgatgttcc cgccgacttt tgccatatcg
    23101 aatttcaagt tcaagatcgt gatgatccag tagataacta aaatacaacc taaaacgaac
    23161 cagtgattat tgccaagttc tacattacca aatgtgtttc ctaacaatgg tcctaaagta
    23221 gaagcgacca ttaccatacc tgggaacatt tgcacccata atagccatgc aacgacaaat
    23281 ccccatttgc tgcccagtgc tgtttttacc cataactgag gaccgccttc ttgaggaagc
    23341 atcgtggaaa gctcaccgga aaccattgat atcggaccgg caaaaaacaa aactgcaaaa
    23401 attgaataaa aaatcaatgt ccaaccgact gcggctaacg taggaatact ccgaaccgtt
    23461 gcacacagtg ccattgtcag accgataaag gtcccaagac ccattttact tacttcagat
    23521 ttactcaccg ttgacactct ccttgatttt ttggataaaa taacggtgtc tttgaagtag
    23581 tttcagcaat aaacgaaaca attcagcgaa tatttgaggg gattctttga gtttattgct
    23641 tgaagggttt cttcaaagac aacctcaaaa caaagggatc gaagagctta ggtctcccct
    23701 tgctcttcag atccaaacat gcgtcagaaa cagttagtat tcaagctgtt ttctaacatg
    23761 attaccttgc ttattttgct tcgcttgcca ataattggtc tgcgtagatt ttttccaatt
    23821 tttcttgaag agctgctttg attttttcag catattcttc aaagtgttct tctttgttca
    23881 tgtatggtgt cattacgctg gcacgcaata cagttacttt tccggcacgg ttccattctt
    23941 catctgagaa tcctagttgg ttcacaaatt gtaatgggct gttgccatag tctgggatag
    24001 caaagtcagt atgtgaagtt aagaactcat taccgtaaat gcttcctttc acgtatgaag
    24061 aataatcata tacgtcatgg ttcaatttat tcattgcaac taagtcatcg ttgccttttt
    24121 ctttgaatac atagtctacc atattgaaat ctggataagt taatggatga acttcgattt
    24181 ctttgtctcc tactttaaat gacaagtcat tcaagaagtt gtagaaacga tgtgcacctt
    24241 cgattgatgc acccattaat ttaccatatc ctgtgacatt caaaggtaag acatggtgtg
    24301 ctgcccaaac gctggctgct gttgcccctg cttttgaacc ttccaagata taagccccaa
    24361 gtaatgctgg gatatctgcg cctttttcaa atacatatgt cgcaaagtaa gagataacgt
    24421 ctctcatgcg gatatctttg atgacgatac caccggcaga atatggaaca tagcccattt
    24481 tatgtgggtc aatcgttaca gattctgctt cttcgattgc tttatatgca ctgtgtactt
    24541 cttctaagat ataatctttg ttttctgtaa agacattgta tttgtaatga acatctttta
    24601 aatcttcaaa tggaatgaag ttgttgtctt cgtctaagaa aatcgcacgg ccgtatccgc
    24661 cataagcagc atctacgtgt aagtagaagt aaataccgtc tttttctaat acgcgacgca
    24721 atgcaacgat tttatcgata ccgtcgattg ctccttcttc tgttgatcct acaacaccga
    24781 ctacacctag gattggtgtt ttttcagctg ctagaccacg aacgattttt tcaagttcgt
    24841 tgatgtccat gcgatagttg tggtcaactg gcactggaat cacttggtct aaaccgatac
    24901 cgatgatgtc ggcagctttt agccatgaat agtgtttagt ttgtggaacc aaccattttc
    24961 ctaatttttc taaatgtttt ccgctacgtg ctgagtgagc tttgatttcg tcgatttttt
    25021 ctgggacgct gtctaacaga ttcatgattt cttctgttga caagttcatt aattcccaat
    25081 cgctttttcc agcaactagt tcaggtgtca cttctttcat tgcaagcggc aatgatttga
    25141 tgttgcgggc ataccaaagt ccttctaagt ttgctaaaga accatcagca acgatgtggc
    25201 cccagccatc tttatagctc attaattttg caaattccat tcctacttct tcttccattt
    25261 ggcttgttgc aggagaagat tcataggcaa cgttattacc attccatagc atagcaaaat
    25321 tgtacgctag taaagatggc atcaatgttt cagagttcat atgaccccag taacgtccgg
    25381 cattatgcca aggaacggaa tgggtacgca tccgcgctga aatttctgat agaacatcct
    25441 ttgttttatt cacagtgtgt tcaaaactag cactgctttt ttcttctggc gtaataatag
    25501 gcatgtcttg aggcatgtag ttttgacgcc agcctaaatg ttcatccacc aattcattca
    25561 acaaagcttt atagatttgg ccgttctcag ctttgtcccc aatgaatagg gcatttaggt
    25621 ttagatcttt cgacaatgat tcactcatag tgaaaccctc ctaaaaaaat atttaaattt
    25681 tgtcgagccc tacattacac ctgttcttct attctgtaaa ctaagttttg gcctgatttt
    25741 taatcatatt ttaatatgta tcaattatcc gaattgttca cacttttgaa ataattaaaa
    25801 aaaacgaggg aatttacaaa tgtaaactcc ctcgcaaaga ttgtattaac gacaaccagc
    25861 gttctatttt aggatgggtt tcaaaattgg aatgttgtaa tagtatttcc ctttttcaag
    25921 gtaaaaactt actaaattct aatcaattac tttggccaaa aagtaatttt tcttaccttt
    25981 tcgaataacc acaaacttac catcgaattc atcggatgga tttatgacaa aatttaaatc
    26041 ggtaacgcgg tcaccgttaa tacttatagc tccgtttgaa acatcttcac gtgcttgacg
    26101 tttagatggt tcaatttttg tggaaacaag cagttccacg atattttcag gtgtgcttga
    26161 aatttcaacg ttcggcattt taccgaatcc ttgcgcgatt tcttccgcat ttagttcttt
    26221 aatatttcca gagaacaatg cttgcgttat tttttgcgct tctttcaagt cttcttcact
    26281 atgcacaaat cttgtaactt cttctgccaa acggcgttgt gcttcacgtt tctcaggttc
    26341 tgtctctact tttttcgcta agtcttcgat ttcttcttga gacaagaaag taaagaattt
    26401 caagtatttc actacatcgc gatcatcttg attcaaccag aattggtaga actcaaatgg
    26461 tgaagttttc ttcggatcaa gccaaacggc accgcctgca gtttttccga atttcgttcc
    26521 gtcagccttc agcatcaatg ggatcgttaa accgaacgct tctgcttctg gtccttctaa
    26581 ttttcggatt aggtctaatc cagctgtgat atttccccat tggtctgctc caccgatttg
    26641 caattgcaca tcgtatgttt tatgcagatg caaaaagtcg atagattgca ggatttggta
    26701 agtaaactct gtaaatgaga ttcctacttc caaacggctg gcaacgatat cttttgctaa
    26761 catcgtgttg atgttgaagt ttttaccgta gtctctcaaa aattcaagta aagagatttt
    26821 tgacaaccag tcgtagttgt tcacaaatgt gatatttgca tctttaccaa acaattttct
    26881 catttgattg gaaagtgcgt ccacgttatg ttgcacttgt tccatcgttt gcaacacacg
    26941 ttctgttttg cggccgcttg ggtcgccgat cgttcctgtt ccgccgccaa tcagaatata
    27001 tggatgatgg cctgccaatt ggaatctttt catcatcata aatggaatca aatgtccgat
    27061 atgcatcgaa tcaccagttg gatcaacgcc gcaataaagt gaaatgctct tttcttcaac
    27121 gagctcgcga agtctttctt cgtttgtttg ctgattaatg gcgtcgcgcc aagttaattc
    27181 atcaataata ttcattttac tgacctccgt gtagtttttt tatgaaagca ggaacctgct
    27241 agcatatgta attaattatt tcttcaggat aggataggaa ataaaaaaag tcccttggca
    27301 tataagccaa gggacgaaat ctcgcggtac cacccaattt gtatgtctag atatccaaca
    27361 tacctctcga acatttaacg ctgtcgcgtg gcaatcactg ccaaagactc ctgaaatgta
    27421 attcgcttta aagcatgtac tggtttgcag cgaccaccag ctttctgaaa cattggcttt
    27481 aacaactact tcgtttcgat cttcgtcttg ttattattaa tatgtgtcta tcatagagcg
    27541 caactttttc tttgtcaaca gaaaaaggat atttttttta aattcctcat tttttctttt
    27601 ttgaatccgc tctaattccg aacattttat tttcaattat tgttaaaaga aataactttt
    27661 tctgtatttt ttctgcaatt tatcgagaag tcactttact ttgcctctta ttctttcttc
    27721 gttacaataa ggaatgggac aagtaaaaaa atgaaaaagc aagggacgag gtatatggaa
    27781 aaaaagcaaa aaattatttt aggagccact tctgctttaa ctatcgttat tttaattcta
    27841 agtttgtttt ttgtcatccg atcatctaca aaaaaagaac caatcattcc tgaaaaaaaa
    27901 ataacagaaa aagcaaaaga accgacagat gataagcaac aactagttac tcaagcaaaa
    27961 aatctggctc aaagctacga ttatgaccaa gcgattgcgt tattggaaaa agatagtaag
    28021 aacggatcta ctgaaacaca acagcttctt caaacactaa aaaaggagaa agaacaactg
    28081 atcgactggt ctgatcctac tcagatctct catgtctttt tccacagctt gatcgtggac
    28141 ccgcaaaaag ccttccactc tcaacaggca caaggataca aagattatat ggtgactgtt
    28201 gaagaattca accgttcgat cgaacaactt tatcaaaatg attttgtttt agttaatttg
    28261 aatgatttga tccaaaaaga cgaacaagga aatctttcct ttaacgggct tgcgctacct
    28321 gaaggaaaaa aaccattggt tctttcacaa gatgatgtca gctattatga atatatggat
    28381 aattccggtt ttcctagccg attggtcgta tcaaataaca atgaaatcaa aaatgtctat
    28441 aaagagaaag ataaagaaga aatcggtgac tttgacatgg ttcctctgat cgatgccttt
    28501 gtcaagaaac atcctgattt ttcttatcga ggcgcaaaag gtaccttggc cctgacaggt
    28561 tacaacggag ttttaggtta tcgaacatca aaatctcaat atggagacaa tgaaaaaaca
    28621 aaaaaagaga ttaaagaagc taaaaaagtg gcggaacagt tgaagaaaga cggctggagc
    28681 tttgcttccc atacatgggg tcacttgaat atgactcaag cttcactaga agatgtaaaa
    28741 aaagacaatg aattgtggca gcaagaagtt gcacctatcc taggaaaaac aaatattctg
    28801 atttatccat ttggtgcaga catcagcgac tggcagcctt attcagcaga aaatcaaaaa
    28861 tttgcttact tgaagagtca aggtttcgat attttctgta atgtcgatgc ttctacacca
    28921 gcttggggac aattaggtac ggatttctat cgaaatgctc ggatcaatat cgatggcatt
    28981 cgttttcaat ctgatttaga cggaaaaaat cctattcttg atccattcat caatgtccat
    29041 gatgtctatg acaaagaagc acgcgaatct gcttgatttt acgtcattta tgaaccaaaa
    29101 aagccgaact gacattttat cgtcagtttc ggctttttta tgctttattt cttcttcggt
    29161 gcgattccct tgatcaggac ttctgtccat tctgctgtat atggaagcag ttttgcgtat
    29221 atctccggct tagaaattcc ttctaccatt gcttgaaaat agaggatcaa gaactcatcc
    29281 gaatatttca aatccacttt tccttctttc cgccctcgat ggaaaagatc aagcatgata
    29341 ctgaaacttt cttgcgtata cgcatccatc gcgtcagaaa attctttttt ttcttttttt
    29401 gtatagtatg tcatcaaatc gatataaaat tgtttgttca ctcgattgag ataacggatt
    29461 ttgttttcac tcatggcaat cagtgtttct tcaaatggaa gattttccgc aatcaacgct
    29521 ttcgcttctt gccctaaatc attcataaat gcaaaaaata cttcataaat cagcccttcc
    29581 ttgctgccaa aatatttaaa aatcgtcgct ttacttacct gagcgttctc agctacctta
    29641 tccattgtca ctccagcggt tcctatgtct gtgttcatca agtcaaaagc tgcttgtaaa
    29701 atctctttct ttttctcttg tgctctcttt tcaaaaccgt tcatttgtag agcctcccgt
    29761 aattttaaac ttttataata tatatagttc aaatttattc tgaggtttat tataactatc
    29821 tattgagaaa gtatcaagct agcaattcct tgtttatgaa ttcattgaca gaaatttata
    29881 aaaagaatat tatgtactaa aataataaaa aaagttttat aaaaaggtat aaaacctttt
    29941 tattaaacta tttaatcttg tttagtatat atttttagaa aaaggagtgg ggtttttatg
    30001 gcagaaatcg tcaaagtcac acatttgaaa aagagctttg gtcgtttcga agctttaaaa
    30061 gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat ttatcggacc aaatggttct
    30121 ggaaaatcca cgacgattcg gacactttta gggattataa aaaaagacgg cggaacggca
    30181 acgatttttg ggcaagatgt atgggaagat agtttatcta tccatgaacg tctttcttat
    30241 gttccaggcg atatctcttt gtggggaaat ttgactggag gagcgatcat cgatctcttc
    30301 atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg aactgattca acgtttctct
    30361 cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa atcgccaaaa agtgggcttg
    30421 attgctgctt tggctgttga ttcggatctc tatttatttg atgagcctac ttctggttta
    30481 gatcctttga tggaaacgat tttccaagaa gaagttgaaa aattgaaaga acagggcaaa
    30541 tccatcctac tttcttctca tattctcagt gaagtcgagc gactagcgga taaagtcgtg
    30601 atcatacgcg aaggaacact cgatgaagaa ggaacactcg atgaattgcg tcatttgact
    30661 cgttcgacca tcactataga aacagaagca gagaatagtc acttagcaca attgaacggt
    30721 gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg ctgctgataa cgaagctttc
    30781 gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa aatttgaatc tattccaccg
    30841 actcttgagg atttattcat gagacattat gaaggatgag gaggaagcga aatgactgat
    30901 aaattttcac gctggtccac ccttttttct caaaacctta gccgtgactg gaaaaagatc
    30961 ctgatatggc tagtcggtgt cactgctttt tctggtggtt ttgtccctgc gtttgaagaa
    31021 ttgacttccg gacaaggatt agctggatta tacgaaacct tgcaaaaccc ggcaatgatt
    31081 gcaatggtcg gaccaactcc agcttcgaat gctgctgagt atactttagg agcgatgtat
    31141 gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg tgatctctgt tttacacgtg
    31201 gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg aacttattcg ttcctttcga
    31261 gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa gtattctgat caatttgctt
    31321 ttagcaatat cgatcagctg tacgatgatt tttttccaag cggattcgat cactgcagaa
    31381 ggtgcttggc tttttgggct atctgtaggt agtatgggga ttttggggag tgccgttgca
    31441 ctgtttgctg cacaactgat gccaagttca gccagttcga atggtctgtc tttagcgttt
    31501 ttaggtggac tttaccttct acgagcaata acagatgtag ctgattcctc cctttcgatg
    31561 tggaatcctt taggctggtc ttatttgacc tatccattta caactaacaa ctggattcct
    31621 ttgctttatt tgtttttgtt cagtctaatc ttgataatca gttcatttgt tttagaggaa
    31681 catcgagatt tgaacaccgg ctatatcccc gaacgtgaag gaaaaccgta tgctaaacgt
    31741 tctcttttat ccttgcgtgg tttattattc tatctaaata aagggatgac aatcagttgg
    31801 cttatcactt ttggtttgat gggggcggca tacggctcta tctacgggaa tatgcaacag
    31861 tttttagaaa gtaacgactt aatgaaacag atgtttatcc aatctggcgt ctcaattgaa
    31921 aaaaacttta caagcacgat catggtagtc ttgatttgtc tagctgctat tttaccaatt
    31981 gctagcatca atcgtttatt tacagaagaa acccacggac gtatgaatca gctgttcgca
    32041 acaaaaattt ctaggaacca actttattgg accaatatgt tactagctat tctatttgct
    32101 atattggcaa tcctgattag ttctggtgca ctagggcttt ctgctttggc ttcgatgaag
    32161 gatgcgaaag acatggatct attgatgttt cttaaagccg ggttcaattt cctgcctgcc
    32221 gttttagtgt ttatcggaat tagtggcttt ctattcggat ggcttcctcg aatcggtaaa
    32281 cttgcttata tctacttagc ttactccttt gcactcaatt actttggcgg aattttggat
    32341 ttaccagaat ggttttccaa aacagctgtc cctagctggg ttccacggat gccaaacgat
    32401 tcttttgatc tttccgtgtt ccttacgtta tcatgtatca gccttgcact gttcgtcctt
    32461 ggttctattg gttataggcg aagagactta ctagaaggat aataaaaaag catgtacaat
    32521 ttcatgctag cctccaaatc tgggtcaaaa atccaacatt tggaggtcat ttttttattt
    32581 acaagtcttc taactgctct ttatcaatga tccagacttc tcgattcttt ctttttacat
    32641 aaccattctc ctctaataat ttaaattttc tagataaagt ttcgggggtc gtaccgataa
    32701 atgcagcaag ttctttcatt ttcattggca ggaaaaagtg atccttccct gtcgctttag
    32761 ctaagtcaag cagatagaca gcgactcgtt cttctacttt ttccatcatc aaaaaagttg
    32821 tctgctgctg tacttttgtt aatttctcaa cgttgattgc taaaagtttc agactaaggg
    32881 cgggataatg cagtaagagt tccgagaaat ctttttgtct caatatgcaa atttccgttt
    32941 tttctagtgc ttcgccaaat aaagactcat ttggaacgcc tagcaactgg ctctctccct
    33001 catatccgcc cggctctatt actcgtaaaa gctgttcctt tccgctagca gataattgat
    33061 acactttcat actgccggag gccacgatca ctaattgagg ttctccattc ggcgcaagaa
    33121 tctgttctcc tttttccaca tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt
    33181 gctgtactaa gtgattgaac aaaggaacaa atgaaacaca taaatgatca tgactcataa
    33241 tctctcctcc ttctaaacag ctatttctaa taaagaaaca taacttccct tattccaact
    33301 gataattttc ttttatcata gcataaacgt caagcgaacc agataccagc aaatagagtg
    33361 gatttctttt tgtcccttgc tttattttcc ttcttttgag accttaagta attgattcct
    33421 atcaattttt taaatattta ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg
    33481 agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt ctattgaaat ttttccgcac
    33541 ctagcttttg atatagtggt gctagttctt gtaaggtttc agcggttatt tgctcaacgt
    33601 gttcgatttc ttgcggatag aagaatagtt tacccatttg aaaggttcgg tctctcatcg
    33661 cttttttcaa ctcttcttgt ttctttgtat cggacaaata gtcagacaaa gctaagtggt
    33721 cgtcaagttc atgttctggt tgcggccata tgtaataatc cttgatatcg actgttttgc
    33781 tccactcttt cagtatcgca agcaactggt tatacgctgc aatgctacca tatcgttctt
    33841 cgcttttata atgttggtac atcaaataga tttggtattg tttttgattc aacaaaactg
    33901 ctagacaggc attctcgtct tgacgagatt cgcttcggta cgcgctccaa aaatgactgc
    33961 gtaaattcca gccattcgtc cagctttcga ttttaggttt gctcatgccg tacatatcag
    34021 gcaaaagtgc agctgtctgg agctgaatct ctttccattt ttgccagtgt tctttatatt
    34081 ctttcttgat ttgttcaatc ttatctgttg gatggctctc tttgagctgt ttgaatgcgt
    34141 agattggtcg atcaaacaac tgataatctg attttccaag ttccattgtt tcgcctcttt
    34201 ccgttatctt ttgtattcta cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa
    34261 gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca
    34321 gttttattga acttaaggtt gattgatcac atctaaaagc gttgaaaata aaatggcact
    34381 agaagcagct cctgggtcga tatgtccgat tgcacgctct cctagatagg aagctcgtcc
    34441 ttttttagct accaagactt tggtgtgttc ttttgcctgg tcaatgattt cttttgttaa
    34501 attccctgcc ttcaatgcct ctgtgactgg cagccaaaca tctaacatcg ttttatcgtc
    34561 agcttctgcc tttcctcggc tttgaatacc cgctgttccc tgctcgatca cttcgcctaa
    34621 tttttcttga gaatcaatag aatcgacctc ttttgtcgct ttcatcatgt tcatgaatgc
    34681 agatccgtat aacggaccag aagccccacc gactttcgat atcatcgcca tagagaatac
    34741 tttgaatgta tcgctgattg tttcaggatg atcggcttga aaagcttctt cataagctga
    34801 aacccctctt cccatgttat ttccatggtc tccatcgcca atcggggtat ctagatcact
    34861 gagatattgt ttgttctgat tgattgcctc tgcaaattgc gtgagccatt cttggatttc
    34921 ttgaacggtt agtttcatat tatcaccctt ttatcttgag attttttacc atgaaatcgt
    34981 ctgtacattg ctttctaaag aatctttcca ttgtgtctcc gtcaagtcga tcagtgttag
    35041 agaaagacct tgcatatcta gtgaggtcat aaaattaccg acttttcgga aaactacatt
    35101 gacgcctctt tcttccaaca gagctaaaac atcattcatg aacacgaact gttccatcaa
    35161 aggcgtcccg cccattccgt tgactagtac acctgcttct tttggtaaat tcgagtaatc
    35221 atctaaaatc ttagtaacaa gttcctttgc cagtacttta gaaggctgca tcttttctcg
    35281 acgatagcct ggctcaccgt ggataccaac accatactcg atctcatctt ctcctagttc
    35341 gaatcctggt ttccctactt ctggtacagt tgctgctcga agcgctagac cgatcgtctt
    35401 agttgcctgt acgacctttt ctcccagttc tttcaactca gctagggaag caccgtttct
    35461 tgctgcatcg cccacgattt tgtgtacaag gacagtacct gcaactccgc gttttccagc
    35521 agtatacgta ctattttcaa cggcgatatc atcatctacg atcacacttt ctacttcaat
    35581 atcgtccata gcagctaatt cctttgccat atcgaagttc aatgcatccc ctgtataatt
    35641 cttaacgatc agcaaaactc cttttccact atcggcttcc ttaatagccg tctgaatctg
    35701 atctggagtt ggagaagtaa acacatcacc aagcaccgct gcacttaaca tcccttcccc
    35761 tacaaacccg gcatgggatg gttcatgtcc gcttcctcca ccagaaacca aaccaacttg
    35821 ttccgctttc tgattcttgg ctacaacacg ggaagattct acacgatgaa caagttctgg
    35881 atagcttttt actaatcctt ccagcatttc ttcgacaata ttgcttggat cattcatgat
    35941 ttttttcaat ttattcccat cctttctttc cggcattttg tgcttctgtt aagatttgct
    36001 gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa agctccttcc actaaaggcg
    36061 catccaccag atgataacgt tgctgctgct cttcttccaa catgtctttt gccagttccg
    36121 agttcaatac cgcgctaccg atatccgcaa aaacaaaata actagctgca tcgcttgatt
    36181 cattgactgc ttctacgatt ttcatcgggt ctgatccgat ctcgccattt attccgccga
    36241 gagaaaaaat ttgcacctct tcggattttt gcatctgctc aatcatttct ttaataccat
    36301 ctgttacttt ttgactatga gaaataagca aaatactttt tttcacaaga atcttccttt
    36361 cttttacata gttgctaacg attggtttcg ctttcattaa tgaacaattc aagtatataa
    36421 taaatttcta attttgggaa aatatgtgta gtgcttcttg aagtttgtta tactttaatt
    36481 aaaagagagg agtgatcgaa tgattacaac aacaacgaac agtgtagagg gaaaagccgt
    36541 agtagcttat aaagggatcg tttttggtga ggtcattact gggatccatg catttaaaga
    36601 cttggaggcg gggttgcgaa atatctttgg cggccgctca aaaagctatg aaaacgaact
    36661 aatgggtgca agagaagaag cacttgccga aatggccgaa cgtgcaaaag cattaggagc
    36721 tgatgcgatc attggcgtaa aaatggacta tgaagtatta ggcagcgaca atggcatgct
    36781 gatggtcaca tgtagtggaa cagctgtctc cacgagataa gtaggggaca tctgttttaa
    36841 taactaaaga gagatgggac atgtagtggc actcaagaat tagatttttc agtctaactt
    36901 cgaggtactc taccttccca tcctctcttt tttatttctt tttgcggatg attccgtaag
    36961 ctgctgcgac taacacaccc gcccctgcta aggaataact gacagaagct gttttttcat
    37021 ttgtttttgg caaagtctga cttgttgatt tggatggctc tgtcgtttgc tgaactgttt
    37081 ctgtcgtgct tccagaggtc actgattttt ctcctgccga atgcaaaata tcttttattc
    37141 gttggaaact agctgctaag tcagcttttt ttgcttcaaa atcattagca actgtttgtg
    37201 cttcgctgat ctttccgtgg attgcttgac tggccgctgt gatatccgct tgagtttgac
    37261 ttagctttcc agaagctgtt tgagcaacct gatttaattg ctgattgaat tcttttgctt
    37321 gttggatctt cccgtcaaga tctgtttgga cagcttccgc ttttgctttc gcttcggcaa
    37381 gctttttctc aagttgtgca cggtcatttc ccactttatt tttaagatca gctaaatctt
    37441 gatcgattgc tgcttggtct tctttcaatt tgttttttgc agcttctaat gcttcttcga
    37501 gttcttttct tttattttcc aagtcttgca aatgatcgat ttggtcagaa gaggtagaac
    37561 tatttattga tgtatcttct gaagatgaaa tcgtttctcc tgaattatct gtcggtacat
    37621 tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc agaagagctt atggttgttg
    37681 aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc tgtctgtccc aataagaaaa
    37741 tactggaaac agcaattggc cagattaacg gcttcattct ttttttcatc acttttccca
    37801 tccttactac tttttaacta atttcttatt ataagcaaaa atgtttggaa gaaccataaa
    37861 cacaacaggc tgtttgtcta aaaaacggtg catcttttat ttttttgtac gttccgggct
    37921 tttccatgtc ccgcttccat aacctaaaat cgttttgact gctggaatga aagtcgtcac
    37981 gatcgttatc gcgttcacta tccagaacaa aagcatatat agtggtgcga aaagaaggta
    38041 tttcaatttt cttcgtcgat catctacaat cagcgaagct agcaactgga aaaatcctgc
    38101 gaccatttca aagcagatga acacaaaact gaatgtaaaa gtaatataaa tctgttcgta
    38161 gtttccttga tatacataat gcccgattaa tccagcaaaa agaactacgg aaatacaaaa
    38221 gaatattgac cagacaatac tcaatgtttg atcaatgaag atgatcgttc ttccaatatg
    38281 tttaaaagga tggagcatca cttttttgaa attcgtcagc cagacttctg ttccaccttt
    38341 tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc acaggtactt ccatgaaaaa
    38401 gataatctca ggagcaaaaa cagataacca gtcatttaac tgatgatccc aagcaatact
    38461 gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca tcgatcaagg cttcttttcg
    38521 atacatcgta tttgctccac tgtacgcata aagaccaccg aaaacagctg actgtgttcg
    38581 tttaatgatg cccacgatac ttgagaattc aaccgtttga gattttgcaa tcaatttggt
    38641 acgattttgg acatccatat ttgcagtgac cgcagcaata tgtcttgcac cagggcgaat
    38701 gaaataattc acatacctaa tcagtgcatc tggctccggt accgtgtctg catcattgct
    38761 taaaatcaat ttcccttttg caaaggcaag tccgatatta aacgcgtggg cttttccttt
    38821 atttttttcg atccgaacca cacgaagatt agcgtatttt ttcatcagtc tagctaatat
    38881 ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat acctcatagt tgtgataatt
    38941 caatttcgtc atcaaatact cgattgtata ttcgatcaca atttcttcat tatgggctgg
    39001 caccatgatc gtgatgaacg gttcgacact caagggaaca tctacccatt ctttttgctt
    39061 atgtttgtat aaaaacacat aacaccagac accaataaac catacaaatc cgcctaaaat
    39121 cggataagaa acgagaaaaa aatacataat agacaaacaa gtatctaaga ataaattacc
    39181 tgtatgggtg attgagatca tttcagttcc accccatttt ctttatacaa atctgcaatg
    39241 aatgtcgtat ctagattttt ctcttcggag acagaataaa aacggaccgt ttctctctct
    39301 tcttttgttc caaaacgttc cgtatacact tcttcaagta attcttgtct ttttttcaat
    39361 ttttcatctt catattcaaa agatttattt acttttttgt atagatttcg attgttccac
    39421 ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt
    39481 aagaaataca gcattttctt ttctgataaa aaagcgtaca aggaaaaatc cttggctctt
    39541 tctggtctcg ttaatgggaa aaagacccat ataaaaggaa gaacaacgat catccatcct
    39601 aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat taccttttga aaaatactta
    39661 tctttcacca aaattgatgc aacttcttct cttgttactt ttttcatatc ctcacctcac
    39721 gtttttttgt tacgacgtag gagtttacca tacatttcat ttcttttcac ataaagtgtt
    39781 cctaaatttt tcctaagaaa aagaacaaaa catttctcat cagcaaatga aaaatatttt
    39841 gttcttttta tcagttgttc tttttatttt ttatctctta attatgttgc tctttccaat
    39901 acgacaatgc ttttcattcc tctagatatc gccgtgtaca tttgtctgcg tcttctttgc
    39961 tcatctccat cgttttcaaa tggatataag attacatgat caaattccag tccttttgct
    40021 agactcaaag gaatgatttg gcagttttct aatccatatg aatttttttt aatcgattct
    40081 tgtagcgttt gggtttcctc ttctgtttgg gtgataactg ccgctttttc tccttttaga
    40141 gaaaccaaaa tctcagctag tattttcaac aattcttcag aggatcgaaa agagcggtat
    40201 tcgggttctt ttccttgcgg tcggatcgat acgatgtcga tcttctcttg atccacagca
    40261 taggttttga aaagctctgt gattgctccg ctagaacggt aactatttct aagatcatac
    40321 cgatggatcg gcagatggta gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa
    40381 gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa agtgagtttt tggaaataat
    40441 tcgatcaata gacctaattg tgccagggta taatcttgta cttcatcaat caatagatag
    40501 cgaaatgctg gtacatttac cttttcaaca agtaaatgac gaatagctaa tataatgact
    40561 gcttcatcaa cagtacgtgt gggttgccaa gcataagctg agccatatcg tttttcataa
    40621 attctttcaa acaactgatg ctcatccacc catgccattt cttcgatttg tctggttatt
    40681 tttcgatatt ttttttgtaa aagtttcctt gcataagcta caatactttg ttccgaatca
    40741 tcagaaatca atttaccgaa taattccaac tgcctatctt ctgtcaacga gagtacctga
    40801 ttctgcacag caggactttt tgcttgtttc aaaagatgat tcttccattc gctcatcagt
    40861 gcctttttcg ttgcttgaag cctatcgatc aatttaggat gagcaggaac tttctcatat
    40921 attttttcga tcgttttttt agaaaggact gtttttccat ttttagtaag tccacggata
    40981 aaattcggat gatctaaaaa caaaggatct gattgtttta gattatcgat aaactttttg
    41041 ctacgcaaac gttctgtctg ctccgataca ttttctcccg tgatacgttt gaaataagcc
    41101 tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca gctgtatgat ggttagattc
    41161 agtggatttc tttcacctaa tgatggtagg acattcgaaa tatagtcgat aaaccgatga
    41221 ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat ctcgatataa atagagcaga
    41281 tacgcaatac gctgcatgat agcagaggtt ttaccacttc ctgctacgcc atttaccaag
    41341 atattttgac ttttcacatc ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg
    41401 atagccttca tttcattggt ctcatcttgg cctaagactt ctaataatac gtcatcttga
    41461 atagctacag acgtatcaaa aaacttgacc aaacgatctc tttccaaaag aaattgtcgt
    41521 ctttctttaa taaccgtatc gattttattt tgacgaaccg tataagaaga tggaccaatc
    41581 atgttattgt aaaaaagttc ggcaattggt gaacgccaat cataaacaag attttcatct
    41641 gcatcatctg tgaaatttgc tgtaccgata taaaaggatt cttttggctc attatccaga
    41701 aagtctacaa cgacttttcc aaagtaggga cttttcaata aacgttcgat ctttgctagc
    41761 tgattttctg cgtttttctg tttgatattc atctgatcga tttctctatt tttcatttca
    41821 atcatcgaaa aagtatccag gttatctaag taactgtcaa aattcaatcg aagctcgcca
    41881 gccatttctt tcaacgactg gtttccttct atttttgccg tatccatcga ttcccgcagt
    41941 tcgttttttt tatcaattag cttttgatac acgttcttca gatatgcttc ttctaactgt
    42001 ttttgtgtgt tgaccataca cattctcctc tttttttaaa gtggtggaac cgattagttt
    42061 acctctttta agagtcgaat gcaagctttt taacgagtat catttgttag gatatatcag
    42121 ttcttcaggt cggacagttt gatataaaga aaatgcacca tcaaggtttt ttacgtggta
    42181 gccagactgc ttcaagattc tttccgctag atagctacgt aatccgctat gacagctgac
    42241 gatatattct tgttggctgt ctaattcatt taatcggtcc cgaagttcat tcaaagggat
    42301 atgtttggct tccttgaatt tgcccttttc caattcttct gctgttcgca catctaataa
    42361 aatttttcct tttgctagtt ctgtcggcaa ttcatgccat tgtacggttt cactaagccc
    42421 ttctgcaatg ttcattgctg catatcctag catgtttact ggatctttgg ctgagccaaa
    42481 tggcggtgca tacgtcaatt ctaattccgg taaatcgaag atcgtcaaat gccctttgat
    42541 tgctgttgct aaaatatcga tccgtttatc cacacctttt gccccaacac cttgtgcacc
    42601 ataaatctcg ccagtctctg gatggaagat caacttcagt aaaatatctg ttgcttctgg
    42661 ataatagctt gcatgatctt ttcctgatac atgaatgact tcaaaagaca gttcgttttc
    42721 tttggctgtt ctttcactta aaccggtgga tgcggcagtc attccaaacg ttcgtataat
    42781 tgctgttcca ataccgcctt gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg
    42841 tctaccttga cgattggctg gtgaagcaag agaaataagt gcatcttttc cagtgatttg
    42901 ctgttttaca acgatagcat cccccaccgc ataaatatca ggaatattcg tttgataacg
    42961 ttcgtctact aggatacccc cgcgtatgcc taattcgatt ccagcggtca ctgctagctg
    43021 gttatcagga cgaacaccaa tggataaaat aactaaatca gcagggatgg tacgcccatc
    43081 atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg acagcatgag aagtaatcac
    43141 ttctactcct tgatgagaca attctttttc tataaaagct gccatttctt catccagtgt
    43201 tggaagaata tgtggtgctt gttcgatcac catgacttct aaacctcttc tttttaggtt
    43261 ttctgccatt tccaatccga tgaatcctgc accaacgatc acggcacgct ttggctgttt
    43321 ttctaatgca tgcatcactt tatcgatatc tggtacattt ctaatagaaa aaacattgtc
    43381 ggcttctgtt atccctggaa tcgatggaac aactggtttt gcaccaggag ataaaaccaa
    43441 tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg acttctaccg ttttgttttc
    43501 gggaaagatg gctgtcactt catgatttgg acgcacatct aaacaaaaac gagcggataa
    43561 tgattctggc gtttggacaa gaagattttc ccgttcactg atttctcccg aaagataata
    43621 aggcaagccg caatttgcaa aagaaacata cggtcctttt tcaaaaacaa cgatttctgc
    43681 atcttccatt aatcggcgaa gccgtgtagc agcagacatt ccacctgcta cacctccgac
    43741 aataacgatt ttcatcttat ttccctccta ctgttcgatc aaaccattga ttcatgccac
    43801 cacgtacatt cactacatca taaccagatt tctttaattc tttagctgct tgtttactcc
    43861 tcatccctga ttgacaaatc acatagacgg tcttttcttt gtctccatca tatctgttta
    43921 tactttgtaa tggaacattt ttagctcctt ttatatgccc gccccggtat tctgcaggtg
    43981 ttctaacatc aagtaacgct ggattttcca aaagcttttc ctttaattct gggactgtta
    44041 tcgattttat tgattgaaat agcaaattca ttttctgcac ctcatttaca tttttataaa
    44101 tacctcatgg ggtatattaa acaggagctt tattaatatg tcaaccagaa tgactcttca
    44161 tttttttcac tactttttat atttgcataa aataccgata aggggtatta tctaattata
    44221 aaaatagagg agaatgtgtt atggagattg ataataaaaa tatcctgaac cgtttgaaac
    44281 gtacagaggg acaaattcgt ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg
    44341 atgtcatcac tcaattaagc gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg
    44401 ttgcagataa tctgaaaaat tgctttgaga aacctgatag taatccagaa gaacaagcgg
    44461 ctaaactaga acaagcgatc aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa
    44521 gtcgttttcc atattgatga attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac
    44581 ctgataaaag cttctccaga aacgacgatt gtcgtatctg taaatggcat cgcgattacc
    44641 ggttatctag actctgccaa tgcagaattc ttagatttgc aaggagtcgt ttttcacgct
    44701 tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt ctttacctga acaagtaatc
    44761 gttgtaccag caggtgtcct tgatttagtt gaactgcaat cccaaggtta tgcttatatc
    44821 aagccttagt cagtagaggt tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt
    44881 tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt tgtgaaaacg ctgtcctgca
    44941 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcgttt ggttgggctt
    45001 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa atcagcggtt
    45061 tgacctaagt attagagaaa aaaacgggaa aatagctcct catattttgc cgttttttga
    45121 gctaataccg aaggtcagct gattgaacac cgtttaagga agagattgtg aaaaagtcgc
    45181 ttagtttcga gtattaagaa aaattttgtg aaaatggatt tctacatttt tgcataaatt
    45241 tggcttaata ccgagaaact ggcttttgaa taccgtttaa ggaagaggtt gtaaaatcag
    45301 cggtttgacc tgagtattag agaaacaaac ggaaaaatag ctgctcatat tttgccgttt
    45361 tttgagctaa taccgaaggt cagctgattg aacaccgttt accaagaggt tgtgagccta
    45421 ccatttaact ccgaatcaca agaaataatt tcaaaaacaa cttctcctgt ttttggttcg
    45481 gttggcttat tatcgagaag tagctactgg gagtctattt atcaaaaaaa ggaatgcgac
    45541 ataggtcaca cccctttttt attttctttg aaacatcttt cggtactcga cagcatacaa
    45601 gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa atgactctca cccacagcgt
    45661 tggtaattct gggaaaagca gcaaaaggat catgccaaca tagatgacag cagtcgctac
    45721 tttgccatac cacttcgctc cacttaattt gatattttta gatagcccga ttaatccagc
    45781 tacaaacata aagccttctt ttatcaagaa tagaccaaac aacagccaaa gccacggtcg
    45841 ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat ttatctgcaa acggatcaag
    45901 caccttccct aagtctgtta cctgattata tctccgagca atgaccccgt ctagataatc
    45961 agacaaacct gagacaacaa tgaccgctcc tgctatataa aatgactgtc tcagcacaaa
    46021 ataaagataa atcggcagca aaaaaatacg gaatatcgat aaaagattcg gaatcgtttg
    46081 tatttcttta cgccagtctg tgcttaattt cattttatcg ccctacctcc tcagcactct
    46141 tttcttttta ttttagctta cacggggaat aaaaccaatg atttcactgt ttttattttt
    46201 ctttacagac actgatatta tgagctaatt tgagataatc acttgctgtt ttcatcgttt
    46261 actcttcttt cttcctgtcg tttgcgcatt tcttttacta gtactttatg agcttttttt
    46321 gatcctcgct ctaggtcacg ctgccgttta cgtgttttat aatcgataaa cagactgtct
    46381 aaatcgtaac ctgtcgggta cagatcttct gctgaacgca ccagacgaac acgctgacga
    46441 ggcacgtgga ttattttttt gtctaaatat acatcaatcg tatcttcacc tatatcttca
    46501 taaactaacg cttctttttg gtattcattg acaaatatcc gatcaccttt tgcaaagagc
    46561 tgacgttctt ttttattctc cttcctattt gtaactgctg tcttaggaag aaattctttt
    46621 ttctgtgtcc gataggcttt ttccgccaag taacgctctg cttgatggat caatttcatc
    46681 gacatatcca ttttatgagc aatccacaaa gcttggcttt ctcctgtttc ttccaacccg
    46741 caactgatat ttaggacgaa gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc
    46801 ttcatgatct ccggcgaatt tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc
    46861 cccttgctca tacatagatt ccatgattgc gatcgctagt gcagcccctt catttggctc
    46921 tgttccactg ccaatctcat ctaaaagtac caacgtattc ctttttatct ttttcaaaat
    46981 agcagcgatg ttttgcatat gaccagaaaa tgtactcaac gcattttcta aattttggtg
    47041 gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc tctgtccctt cttttgccgg
    47101 aacttgcata ccgaacatcg ccatcaaagt caataaaccg actgtcttca acacgactgt
    47161 tttgcctccg gcatttgctc cggtgataat caagcctcga tagtctttac cgatttcaac
    47221 atccaatggt accgcatgct caggtaaaaa tggatgtctg ccttgcttga taatgatatg
    47281 ttccgattta ttgattctag gtgtgcttcc attgatttct cggctaaatt ttgcccgagc
    47341 aaagatgata tctaacaccg tgatcgtttc gatcaataaa tcgatcactt cttcgttttc
    47401 tgcgatagca ccagtcaatg ccgccaggac ttgatattct tcactgattt cttcagcttt
    47461 taacaactga tagtgttcat tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt
    47521 tcttttattt gattcatcga taatcgttcc atctatttta tttttatagc tggctttgat
    47581 aggaatcgta tagtattcgc ctttttgaac aatcattgct tcttggatca tttctttgtt
    47641 tttcggatga cgcaagaatt tcagtaattt tgactggatt tctttttcga ttagttgtaa
    47701 ttgtttgcgt actttacgca aattacgtga cgcgtcgtca ctgactttat tattttttat
    47761 tttctggtca atcaactctt ctacatttat caaatcagga agatgtttgc tataagcaaa
    47821 caacaagggt gtctggtatt gattcttatc aaaaaacttt gtgatcatcc gactgcttct
    47881 aagaaaatcc gcatattcga tcaaatcggt tggctgcaga atcaaccctt ttttgacttg
    47941 ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga acatgctgac tgctttctaa
    48001 gatcaagcgt gcttctcttg tttcttcttg acgatccaag acagtttgta ggtttgtcga
    48061 gaccgtcata tcgctgattc gttttttgct atagtttcca attgctcttg cttggatttc
    48121 ctttttgatc cgatcaaatt gtaattgact aatcatttcg ttgtccatat tctctccttt
    48181 tcttgaagag atacctgtcc caaaataaat aagggcctag agataaataa aaggatagaa
    48241 cacagcttaa aaaagtcatg tcctatcctt attagaatac aaagatattg ttttcctgac
    48301 aggtaccgtt tcataagcac accaaataaa ctttgtttat tggctattcg acttactgat
    48361 tagataccag ctacactcac aaaacatacc ccattcttct aggtcttatt tattttccac
    48421 ttaactatag caagacacat tttggttgtc aaactttagt atgttttata gatttacacc
    48481 attaatgaat agacacctac cgcgataatt gctccgatca ttggacctgc gattggtacc
    48541 catgaatatg cccaatctga agttccttta ttagctattg gcaataccgc atgtgctaat
    48601 cgaggaccaa agtcccgagc agggttgatt gcatatcctg ttggccctcc taatgaaaga
    48661 ccgatggcta agatcaatcc acctacagcg aatacgttcg ttccaggtgc aaattcgttt
    48721 tgtccaaaag ctagcaaccc aagaactaat acaaatgtac caattaattc tgtgatcaca
    48781 tttgctggat aattccgtat agcaggacct gttgcaaacg ttcctaaaat cgcgctttca
    48841 tctttcgttg cattccaatg tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa
    48901 gctcctaaaa cttgcgcaat gatgaaagga acgacaagat tccaactaat tgcccctgtc
    48961 atcgccatag ctagagagac tgcaggattc aaatgagctg ggcccatgaa tccagaaata
    49021 tagacagcca ttgttacagc gagtccccaa ccaaatgcga tgacaatcca gccagaagcc
    49081 tgtgctttac ttttgtttaa attcacagcc gcacacacac catctccaag caaaactagg
    49141 atcagtgtcc caataaactc ccccatgatt tgagtcatat cactgttcat ttatttctcc
    49201 ccctttagtt cccgcagatc tgattctgca atcaatgctt ctaaacgttt agtctgttgc
    49261 gccttctcct cttctgtcca ttcaaaatat ccagcaattg catcgatcac aggttgcttg
    49321 atctcatcaa gctgatctcg ttcaaacaac agatgatttg tacgacgaat gagataatcg
    49381 cctggggcca aaaccatttc ttcttctaaa ccataacgta accgagctga ctcagcgaga
    49441 ctcaaaccag gataaggtgc catttctttt gctaattcaa agatacgtcg ggcattagtc
    49501 ccgtaaaaat cagcgatata agtagcatct tcttcctcta aaccggctgc tactccttct
    49561 tttgctaatt ctgtcactgt ttcttctaat ttagttggat cgaagtttcc acctgaaatc
    49621 tgatattttt tagagtcgat ttctttcgtc tctattccgt attcttcttt taataattgg
    49681 cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat cggtgatttt cccaccagat
    49741 aaagtcagca aaccatctgg ttctctttct aaagaactac ctctggaaat cgtagaaggt
    49801 gctttttcat cacggctgtt ttccaaatgg ttcaacacgt cttctacttc agcacgagaa
    49861 actttgtttt ccttatattc acttactgta tcgacaactt tattgaagct cttgtctgaa
    49921 atcgatccat tatctccacc attgtaatca gaaccagaat taccaatcaa cagtggacga
    49981 agtcctgccc agcttgcttc gatatcattc aatgtgatat ttgcttctgg atagcgatga
    50041 ttgatcacat ccaatagata atctacatct tcttgtgtga ctttcggatc cgtaaaatct
    50101 ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa
    50161 accatccgcc catcttgctt tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt
    50221 ttcgcatcta caactaaatg gatcccttta gttggacgca ttttaggaga gactggacgc
    50281 gtaaaattca agtttcgtac tttatctacc caaggaccac tcgtattgat cactaatttt
    50341 gctttgattt cgatcacttc atctgtcagc aggtcacgag ctttgacgcc aacgatttga
    50401 tcgccatcat ataaaaagcc aaccgctttc attttactta ctagataagc cccatcttcg
    50461 gcagcctttt tgatattatc aatcactaaa cgggcatcat tgttgcggaa atctagatag
    50521 acacccgcgc cttttagccc ttcttttttc aaaaacggtt ctctttccaa tacttcttct
    50581 ggtgtcagcg tataattctc atatttggtt cctgtcacat ttgccagttt gtcataaagg
    50641 tccattgcta ctttgaccga gaacatattg aaggttgttg ctccttcgcc ctcatagatt
    50701 ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa tactttgtac gactgcacgt
    50761 tcaccaactg tgtcagctac tacttccaca tcaaatgtct tcagataacg aataccgcca
    50821 tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat
    50881 cctgttttga ttcctgatgc tgctgactgg actgccacac cggcaccagt gattccgcca
    50941 ccgatgatca acagatccaa ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc
    51001 ttgtttgaaa acatatggat ttcctcctta ttcaaatgct tgggtagctt ttactgcttt
    51061 tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc atttgaactt caaatagctt
    51121 gccagcttca taaaattcgc ggatctcgtc tgtatctttc caataaccga cagctaatcc
    51181 tgctaaaaat gctgccccta gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac
    51241 attcaagata tctgtttgga attgcatcag atattcattg tttgctgcac cgccatctac
    51301 tttcaataca ggaatcttga ttccagtgtc atcttgcatc gtatcgatga tatctctcac
    51361 ttgataagca atcgactgta aagtagcttt aatgatatct tcttttgtcg taccacgtgt
    51421 caagccaaac atagcccctc gtgctgcctg atcccaataa ggagcaccta gcccaacgaa
    51481 tgcaggaaca acataaacct catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc
    51541 gctagctttt tgcaacattt gaagtccatc tctcagccat tgaatcgaag aaccagccac
    51601 aaaaatactt ccttctaaag catagtaaac ctttccattt atgccgtaac caattgtcgt
    51661 caataaatta tttttggata attgcggttt ttcaccggta ttcatgacaa taaatgaacc
    51721 tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc
    51781 ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt ccataaaaat gataaccttg
    51841 cgtataacca taaacttctg agttagaagt gacttttggt aacatcactc gaggaatatt
    51901 tagtaaatct aaaatctcct gatcccaatc tagctgatgg atattgaaca acatcgtccg
    51961 actggcattc gagtaatcag taacatgtgc ttgtccatcg gtcagtttcc atactaacca
    52021 agaatcgatc gttccaaaca tcaattcgcc tttttcagct cgttcttgcg cgccttctac
    52081 atgatcaaga atccagcgta tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt
    52141 tttttcgtgg aaaaattctt gatagccttc attttttaag ttatctgcaa ttccagctga
    52201 ctgtctggat tgccaaacaa ctgcatgata gattggacgt cctgtatctt tttcccatat
    52261 tactgttgtt tcacgctggt tcgttatgcc aatcccagca atctgatttg gtttgatccc
    52321 tgattcgatg aacgctccgg caataactga ctgtacagaa ttccagattt catttgcatc
    52381 atgttccacc caactttctt gcgggaaata ttgcgtgaac tctttttgag aactgccgat
    52441 gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta gttgtacctt ggtcaatcgc
    52501 catgatatac gttgattctg tcattgtgat tccctccatt atatgcaaac gctttcttta
    52561 actactctta tcatactact gttttaaaaa aagaaactgt caaaatttta ttcttttttt
    52621 ctttttttga cagtttttta ttatgaagct attttttaat caattctttt cttttttcag
    52681 cgtctctatt cttcttttca atcgaataat gtcgtaaggt gacgcaaatt ctgacagata
    52741 atattcacac tgttttttgt ttctctggca aaatgtgatc accaaatcat attctttacc
    52801 ttcttcataa cttccaatct caataccgcc gattcccctc agttctctga tcagatattg
    52861 ttgaaaagca atacgataaa tcggcagact ttctaaatcg attccaatca ctactggttt
    52921 tgcaatataa aaatcgatca tcaaaagaac attggcatac tggatcatca ggtaactaag
    52981 ctcactatct tcagctcttt tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc
    53041 cagtaactgt gttaaggaat gcccgagcat tttctgctgc tgttccaaaa gccgatcacg
    53101 ctcatagact tcgatctttc caacaaagaa aaaccattca ttgttgatct gggaaatttg
    53161 gtagcctact gccttttctt ctttgatttt caaacgacga ggacgataat gccataacat
    53221 cgtttcccga atgtaagtat ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc
    53281 atactgataa aatatttctt cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat
    53341 cattgtttcg tatacattca gttctgctgc tgttcgacta agatgcatca cgatgattgg
    53401 atcgattgct tgataaagcc ggtccgactg atagagtaat ttcttttctt tcaaagttgc
    53461 gtacgtactt ttttcattca atagtctttt tttcgtaatc ccgagccagc aagcaatctg
    53521 tgcttctgct gatgccgtaa acgttgtttc taatacacgg tccaatccta gaatgaacgg
    53581 tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga tcttccggga tatattggaa
    53641 tagttcataa taaaaatatc gtatctgtag ctcttctccg tataattgac cgtttttgat
    53701 ttgtaatcca aattcctcta agagctggtt caattctttg atctttcgaa aaacagaaga
    53761 ctcactgata ttaaacgctg tcgctaatcg aacgatggat acttgctgat gttctaataa
    53821 atagacgagc atcttaaact tcaagctttc ctgcaataaa aacaatagaa ttttttccaa
    53881 acttacatct tcgtccaaca caagcagcgc ctgatactca tttcgctgta cttctacttt
    53941 tttttccatc attcggccaa tcaattcaat atcttccaaa tattgatcaa acgaggcttt
    54001 tgacagttgg acatgctctc tcaaaccatt gatagaggct gttccccctt gcagaacaag
    54061 gtttcgcaaa atcaggatct gtaactgctc ttttctttct aataatgctt caatcttcat
    54121 aggcagtatt cgccaccttt gaccacacca tatctggcac acggtattct ggctggaaaa
    54181 atagctcatg ttcttctaac tgttcaaacg tccatccttc ttctactgca attgtcagtt
    54241 ggtcaagaag gtggaacatc tcttttttgc tttggagctg tgctccaaga atcttttttt
    54301 cagaatcaaa tagaaatttg agtttaactt tgctttgatt tattgctgta agcttgtagt
    54361 ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct ctcttcttct gtcaaaccta
    54421 cgcttcctaa gaaatagcca aaccactgat tgccgactgg acgataggtt ccctgatcct
    54481 ttgtctgtga gccactaatt gtcttgctga catttcgtgc tgtacggaca gcgttattta
    54541 ctagagaaac atacattttt tcatttgttg gacgaaaagt tacttggatc gcatcgccaa
    54601 tcgcataaac gtctttttga gacgtttgca agtagtcatc tacccagatc gttccatcat
    54661 cattcaatgt taattgttct tcccacatct gattgtcggg acgagaatga ttagccagca
    54721 aaacaagatc atagttctcc tttctttttt ccgtctctac aatagcggtt tcattttcgg
    54781 tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc ttgatttttc atttccttta
    54841 ctaacggttc tgtcatttca ggatctagat aacgaaaaag aattgacttt cggctttcgt
    54901 aaagatggat ttgttttttt cttgatgcca acccttcagc tatttcgatt cctgcttgtc
    54961 ctgcaccaat aattgcaatt ttctttgcat tcatgatctt ttgctgaaga gccgttacgt
    55021 tttgtatctt tttaactggg tgaataagcg ttgattctgc agaaatattt cgaaactttt
    55081 gatttgaacc agtcgctaga atcaaacgat caaaatccaa tttttttcct cctgctaaaa
    55141 cgactttttt ttctttcaat cctattactg tcttttctgt gtaaacatgg attcgatagc
    55201 ggtcagtcag ttcttctttc gtgatccaag aaagttgttt ttcactggta acctttcctt
    55261 ttaatatcaa attgaacccg ctgggaatcc atcctaactt cgattgcttt tcaatcaaat
    55321 atatttcact tgttggcatc aattggcgaa gataaatagc cgcatgaatc cctgcaaacg
    55381 acccgccgac aattacaatg ttcatctgca ttcctctttc ttataaggtg ttctactgct
    55441 aacttctcaa tcagaaaaca cagtattcat aactcgcttt caaccttatg atacatagcg
    55501 atgcagcaga atcaaccctc tccatcatac cgtgttctag gtctgcttct caataatgta
    55561 ctagtggacc taattgaaga gtatcatttc tgaagagatg tttcaaaaaa tgtactctta
    55621 taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa taggaaacag aaaagaacga
    55681 aaagacgagt tttagcgtgt gtaatgtaat agttagaata gacacatcat ttatttttaa
    55741 ttctttaatg cttcaacaac tgcttcgcct aattgatgag cagattgagg attttgccct
    55801 gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag ctgctccaga ctgatagttt
    55861 gctccttttt ctttcaaagc atcttctgct aagaatggta cttcattcgt tgtaccattt
    55921 gcttcctctt cttcattact gaacccagtc actgtacgtc cagctaaaat agattttcca
    55981 gactcatctg taactggcaa caatcctaca acaccgtgac aaacagctga gaccagccct
    56041 tcatttcgat agatttgtga agcgactttc ccaagatcta ttgcttctgg aaaatcccac
    56101 attgttccgt ggccaccagc ataataaacc gctgcataat catttgcatc cacatcatca
    56161 ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt catcttcata ataggcccaa
    56221 tcgatttcat ccatattcaa aacactttgc ggttctaaag gtacatatcc gcctttagga
    56281 ctgacaaaat ctgcatcgat cccatttttt actaatacat cataaaaatg agtcagttca
    56341 cttaaccaca aacctgtagg acgctcgatc ttttcgtact tttctacatt tgttaatacg
    56401 atcaatactt tcattttccc atcctctttc tataaaaatt gcgttattga taacgtgctt
    56461 tcaagaaatt atataatggg cttggcaaat aaagctcttt atatgcttct tcaaaaggtt
    56521 tcgttacaaa ttcatcctca cggttttcca ataatttctc tgcccagtca ggatcgacga
    56581 tcatttgctg tccaattgct gcaagttctg catatttcaa tacctgctct acatcttcgc
    56641 gattgcggat cccaccgaca ccaactaaag ggatctttcc ttgaatcgtg ttatggacca
    56701 cttcaagaat cgatttatcg ttgtatttat cagaacgagc tattcgatca tacatattta
    56761 atgaaacatg cagataatct agttttgact cacgtaatcg ttcaagaagc cagatcgtgt
    56821 cttcaaaacg gatacctggt gtttcatatt cttctggtga gaaacgatac cctacgatga
    56881 atggtttttt agcatattgt tcaaccgctg agaaaaccgt tgttaataat tcttcaataa
    56941 aacgataacg attttccaag ctaccgcccc attgatcctc tcgtctattc gaatgcggag
    57001 agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg
    57061 attcaatcgc tctacgtgtg gctttgccaa atgcttggat tgtttcaacg atttcttctt
    57121 cactcattgc tcgtggtgtt tccgcatcag gacgttttgc tgcaactgca cttgcagata
    57181 caggctgttc tccagataaa gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa
    57241 taatcgcttt tgcgccttct ccttggattg ctttcgctag ctcacttaat cgaggaatca
    57301 tttcatcatg agcgatgctg agttcacctg gccagccttt tccgatatct tggacatttg
    57361 cagcaccagt aataacagca ccaagacctt tagaacgttg tttataataa gtgatctcat
    57421 cttcggtgat cactccattg aaaaagctga gttgtgtcgt cataggcgac agcatcaatc
    57481 ggttcttcaa ttgaactcct gaaggtaaag taattgattg atcaaattgt gacattgtca
    57541 ttcctcccat aattcgctct gattaaccta tcgagaatca tctatcatta aaccactcga
    57601 ttagttgtac atgcaactaa tttgcttaaa aaattttatt ttttttgtaa cttatctgtt
    57661 gcatcattca ttgtctgggc taattcttta cttgcttcat ttcccaaaac tgctttgtat
    57721 gactgatgaa gttcttccca gctaccatcg acttccttag caaattggcg tccttgttct
    57781 gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt tgacgtatcc tttatattct
    57841 aatttttcaa caaaacgagc aatcgttgat ggggcggaat agagcatttg tgcaatatct
    57901 ttttgtggta ttccatcttt ttcttttaat atcaataaaa taaaaccata agttggtgcc
    57961 agacctgtcc ttctaaacgc ttcatcagct aatttattca acatgcgatc tagctttttc
    58021 acgctaaaga aaagacattc atctaacgct ttttcaattg gcttttccat ttcatcccct
    58081 ccattggcgt acatatcata gtatgatttt tattttttgt caaaaaacac ggaataatgt
    58141 ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg
    58201 ttttcgacaa aactccttct ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt
    58261 tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
    58321 taataccgaa aaactggctt ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc
    58381 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttgt cgttttttga
    58441 gctaatgccg atggtcagct gattgtacac cgtttattga gaggtcgtga gcctgccgtt
    58501 gaactccgag tcacaaggag caatttcaaa aaacagcttc tcatgtttta tgactatggt
    58561 gacttgtgat cgaggagttg gcaggagaat actgtttatt cgtaaagaag gggctgtgac
    58621 aagacttttg tcacagtccc ttatttcctt ttattatagg atttcgccat ttgattggat
    58681 gacttgtctg taccaatcaa atgatttttt ctttaaccgt ttctttgtgc cattcccttc
    58741 gtcatcttga tcaacataga tatatccata acgttttgac atttgtgaag tagaagcgct
    58801 gacgatatca atgcagcccc acgacgtata accaaataaa tctactccat ctttgatagc
    58861 ttccttcatc tgctcaatat gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt
    58921 tccatcttct tctactttgt ctatagctcc gagtccgttt tcagctatga acagtggttt
    58981 ttgataccta tcatataatt gattcaaagc aattctcaaa cccttaggat caatctgcca
    59041 gccccattca cttgatggaa gatacggatt tttaattcct ccagttatta agtttccttg
    59101 gaccccttcc caattttctg gatgagcact gactgcacta gacatgtagt agctaaaaga
    59161 aacaaaatca acagggtagg ctgctaagat ttcttcatca cctacttcta tttttaagtt
    59221 gacacctttt tcttcaaaca ttcgttgcgt atacgctgga taatatcctc ttgcttgaac
    59281 atcactaaag aacaaagaag ttctttgctg ctcaattgtt ttttgaatat catccggatc
    59341 acaagaatac ggaacaagaa gctgatagct gatcatacaa ccgacttgtg aacctttgat
    59401 aatttcatga caagcttttg tagctaaagc acttgctata aactgatggt gtgcaccttg
    59461 gtatttcgct tgttccaaat ggggatggtt ttcttcaatt actcctacgc taacaaaagg
    59521 gtgatgtttt acacagttga tctcattgaa agtcatccaa tattttactt tatttttata
    59581 gcgtttgaat actgtttcag cgaaacgaac gtaaaactga ataagctttc gattaggcca
    59641 tccaccatat tctgtaacca aatataacgg catttcataa tgggaaagag aaacgactgg
    59701 ctcaatccca tattttagac attcgtcaaa cacttgatca taaaaaaata atcccgcttc
    59761 attgggtgtt tcttcatcac catgaggaaa aattcttgtc catgcgatag acatccgata
    59821 gcatttaaaa cccatttcag caaataaacg gatgtcttct ttaaagtgat gatagaaatc
    59881 atttccgtgt cttttaggat aataatactt gctgttggga tcctgcgccg cttttacttg
    59941 ctgatgcgtc atcatatgcc attgatctgt ccatttttct ttaggtaaat cgctgtcgaa
    60001 tgtatacaca tcagacacag ataatccttt ttcatctaca tcccatgcac cttcacattg
    60061 attggccgct gtcgctccgc cccataaaaa tccttctgga aattttttca tatcgatccc
    60121 cctaaacttg ctagttttct tgtttgatta tttttcttat gactagtaat gatatttttg
    60181 aatctactac tatttgtaac aataaccatt gtggtcggat catatccctc tttgattaat
    60241 tcttgcaaat ctacttccat caataaatcg cctttacgta ctttttttcc atcagatact
    60301 gcactttgaa agtatttgcc atttaaattc actgtatcaa taccgatatg gatcagtatc
    60361 tctgctcctt cttcactgac taccccaatt gcatgtttcg ttggaaaaac tgttgaaact
    60421 tccccgttta cagaactgta gatttttccg tccgttggaa tgatcgcaaa cccttctcct
    60481 aaagatccac tagcaaatgt tggatcgttt acttcagata aatcaattat ttcacctgct
    60541 gcaggagtac ttaacactat atcctttgag gattcttctt cgatttcatc cttgtagccg
    60601 aaaagaaaag ttagcataca ggaactgatc atcgctaaga aataactagc aactagccaa
    60661 acaaatcctt caccaatata aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa
    60721 gcaccggctc ctgcggcacc agctactcca ccaaatatag ctccacatat acaagcaatg
    60781 taaaatggtc gtttatattt aagagtcaca ccataaacag caggttcagt gattccaaat
    60841 aatgctgtga taaatgcaga taaagataaa ctttttactt tcttattttt cgttttcaaa
    60901 aataccccaa gtactgctcc tgcttgcgca gctacggcag tcatacatgc tggacctagc
    60961 gtatcacgac cgtagaaaga aagattgttg gtcattactg gtacaagtcc ccaatgtaac
    61021 ccaaaaatca ctaacacttg ccaaaatccg ccgataacag caccagataa aacagggcta
    61081 aagttataaa cagctaaata tccagctgca atcagctgac ttgcataatc tgcaattgga
    61141 cctacaacta ataaaataat tgggaccatc acgatgatac aaatgaatgg tgtcaataaa
    61201 tttcgaatac tttcgtgcaa gcttttgcga atcttaggct caatataaga aagaacccat
    61261 acagctaaaa ttgctggaat aaccgtactt gtataccttg ctagaatcac gggcatccct
    61321 agaaatcgca aggttatgct atcactatat gcactgacaa tcgtaggata aacgagagct
    61381 gctgcaataa ccatagcgat gaaacgatct gtattgaact tttttgcagc agtatatgcc
    61441 aaaaatatag gcaagaaata gaaaaatgca tcagctgctg cattcaaaat gatataagtt
    61501 cctgattcag tagttaataa attcaaactc gtacataagg cgagtactcc tttgatcaaa
    61561 ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag cggcgatcat gtctataaaa
    61621 gagtttaaaa tgcctttttt ctcagttcct ctctttccac tagatgcatc atcttctagt
    61681 ttcatttgtt tggataatac gtcatacact tcatttactg catttcctac tacgacttga
    61741 aacattcctc cagcttcgac tacagtgatc acaccttcta gttgctctac cttctcctta
    61801 tctgccttct tattatcttt tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg
    61861 ctgacatttt cttcgcctcc gacaaatcgg acaatatctt tcgctaattg tgaatagtcc
    61921 atttttactt cccccttgtt aattaatgcc gtagcaatcc ttcttacaag ataagtatag
    61981 ttcttagaaa acggtttctc cattcaatta gtaccccttc taatagcgaa cgaaactgta
    62041 caatagtgtg tatggtttac ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt
    62101 atatcgtttc tcttatagtc aaaggagaca aaaaaaacac gcttgatctt cctttgctga
    62161 tgtataagtg gtatctcaag ctgatactct gtgatttttg tttctacttg gcttttgata
    62221 agacagcaca acaaagtctc ggcatttaac ggaagcataa ctaggtcaga actaggagag
    62281 attagttgct gaatggaatc tatcttttta tggattttta tttgattttc ttctttgtag
    62341 taatgatcaa gcagttttgt tttctttttt tcttcaacta tggtcagctt ttcacgcaaa
    62401 atctgataaa tctcccaaga ttcaaactca tctagaacac taggtagata aatcaaatca
    62461 tcccactcaa aaataagctc ttgctcagta gtcaaaattg catcatattc cgttattttg
    62521 tttgattgat ttttgaaaag atacactggt tctatccggc attctttgat ccacatgttc
    62581 atactttgtt gaatctgttt ttttagtgta ttcaaaatac taattggctg gttactaaca
    62641 atcagtatct ccccttgttt tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa
    62701 taagcgcttt cagctaaatt gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt
    62761 aaataaattt ctaaaggata acgggagatg atcttttgta caaaatgatt catggtatta
    62821 tgtccggcat ccatcctcgt tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca
    62881 tggaacaata acgaaacagg aagatctaaa aaacgaataa tcgtttgctc aaatgctctt
    62941 agattcttct ctatttcttt gtttttttca tcattaatca tcaaataatt taactctaat
    63001 agtcgatttt caattgcttg tttttcttga ttggaaaaag aatagggtag ttctgatgat
    63061 aacttttttg tgagggtctc taccatcgga ataaataatg tatacctgtt tgttttttct
    63121 aaaagttctt ctcttcgaag aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc
    63181 tctcctgaaa caatataatt atatgatata agaacctctt ttaatgtctt ttggacagtc
    63241 ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa accttgcgtg ttggatcacc
    63301 gtttctgttc caacaagaga taagaaaatt ctttttgaat tttcttcagc aatgattttt
    63361 agtccttttg tcgacgaaac ttccagttct aatttaggat tgcgctcgat ccaacgcaaa
    63421 atcgttttta tttgtaatga tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt
    63481 tgcatactga catacccttt ttgaaacaat aagtacgtca caatagttga agctcggaaa
    63541 tccatgctgg aatagatttt atgtttcgtc acctcttgta tgatttgctg tttagtttca
    63601 gattcaattg attctaaaaa ataacctgaa cgtttatttg actggataat gattttcttc
    63661 tcgtagatag caagcatatt tttgatttca gtttggatcg ttttcttgtt ggtacctaaa
    63721 gcttcagcta attcttcgct tgttatccaa tgattcttat ttcttaatac tacaagaatt
    63781 tggagttgtc gtttatttaa gtccatttgc ttacacctcc tctttgttat aaacatacag
    63841 gaaaaacctc tcaaatgaca atagtcactt gcagaggttt aaaaatcatt tgaagaaaga
    63901 tctatattca aggtttttac cattagactg gataatttgt ttataatatt caaaactatc
    63961 ttttttcaat ctcctaaagc ttccgctccc atcattttgt ctatccacat aaataaagcc
    64021 atatcgcttc ctcatttccc ctgttcctgc cgatacgata tcgattggtc cccaccataa
    64081 gtaaccaatg atttggcaac catcttttat tgcctcagca atttcctgaa gatgtatttt
    64141 tagatactca caacgttttg gatcgtggat tcgattcaat tcatctggtg cttcatctag
    64201 tccaatgcca ttttcaacta tgaataatgg taattcataa cggtctgtca attcattcat
    64261 gacataacga aaccctagag gatcaattgg ccaactccaa ggttcgggag atttttcctt
    64321 caaaaaggga ttatcgaaac catttgctcc gccagtatct acgcgcattt ctgcttcttt
    64381 tttatagaca gcgctacgat aataactaaa tgcaatataa tctgccgtgt tagcttgcaa
    64441 gcactgttcg tctccatcta acattactgg tgcacagtct tcttccttcc atgtcctgta
    64501 aatataagct ggataatgcc ctcggcacat gacatcggag aaaaaccatg aattgcgttt
    64561 aaattgcagt gaaccaaata cattctctgg atcacagtca tatgcataag tcgctaatgc
    64621 agaaaagcta cacataatac ccatcttagc atttggatcg attttcttta ataattgagt
    64681 agcacgggcg ttcgctagaa acatatgatg acaggcttga taaatcactt tttttgttag
    64741 tgtagcaatg ggttcaagtt tatctctagc agggaatgta accaatccag cagctgcata
    64801 aggcatacga aaagcattat tgatctcatt aaaggtcatc caatatttca ctttcccttg
    64861 gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg
    64921 ccacccccca tactctccta ccaatgctaa gggggtttca taatgactaa gagtaattac
    64981 tggctcgata tcatatcgat ttaatgtatt gattagtctg tcataaaata cgagaccagc
    65041 ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg
    65101 aaaagctttt aaacctaact ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa
    65161 atcaatcgct tcatgagaaa gataatggag tgattcgtct aaagcaatct cccaaatctt
    65221 tttttcttca ttccactgaa tactaggatg ctggttcatg atcccgataa gcgtatctgt
    65281 cacattaggt aatttgccgt cttccaacca tgcaccttca cattgattag cagctactgc
    65341 tccgccccat aaaaaatttt ctggaaatat ttcctgatcc ataactatct ctcctcgcta
    65401 tcaacaattt cttacttgaa gtatagctcg attcatcaaa acaagatatt ctaactattt
    65461 cggcagaaga gaagaaaaaa attgaatgct actcttcatt tttaatattt atatataaca
    65521 aatacataag acgatccccg aaagaaaaca ctcgttaacc atctaagcaa acgatttatt
    65581 ttacttccaa cacaaatgct tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt
    65641 tcagaggata atttgacaca ataaccctta aagcttcacg tggaatagtg aacgtctgct
    65701 tttcactgga catattacaa ataacgagcc attgctggtc ttcatatatg cgagaatagg
    65761 caaagacatg ctcaggcgtc ttttccacta attcatagtc tccccagacg atgatgtcat
    65821 gttttctacg caaatcgatc aatttcttgt acatataaaa aacagaatct tgatctgcta
    65881 acgcttcttc tgcattgatt tctttataat ttggattgac tcgaagccat ggaattccag
    65941 tagtaaaccc agcatgttca cgggcgttcc attgcatagg cgtcctagca ttgtctcttc
    66001 ctttcgcctt gattgattcc aagatttcct cttttgtaaa tccagaagaa atcctttcgt
    66061 tatacatatt gattgtctca atatcttttg cttccgcgat atcatcgatt gggcaattcg
    66121 tcatgcctaa ctcttctcct tgatagatat aaggggtgcc tttcatcata tggagcaaga
    66181 tagcaaacat cttggcactt tcttttcgat acacatcgtc atttccccaa cgagaaacaa
    66241 ttcgcggaag atcgtgatta ttccaaaata aactattcca tccttcatca cctaaagatg
    66301 tttgccattt ggataatact tttttcagtt cagagatact caagggtttc aaatcccatt
    66361 tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa ttggaagacc atggataatt
    66421 catttctttt aggattggaa taaagtttag cgatttcggt agttgctccc catgtttcgc
    66481 caacagtcat cagatttttg tcaccaaaag ttgcttgatt catttcctga agatactcat
    66541 gcagtttagg tccattactt gtgatttctt tatccggttc tttgccaatc atatcgatca
    66601 cgtccatacg aaagccccca atacctttat cgatccagaa attcatcatt tgataaatct
    66661 cttggcgaac ctgtttattt ttccaattca aatcaggttg acgcttgcta tacaagtgca
    66721 gaaaatattg ccccgttttt tcatccaact gccaagcaga accactgaat attgagcgca
    66781 atctattagg ctcttttcca ttgaccggat cacgccaaat ataatagtcc cgataaggat
    66841 tttctttatt tttacgggcc tcaatgaacc acggatgttc atcagaagta tgattcacca
    66901 ccagatccat gatgatacgg atatttcttt tctttgcttc ttcaatcagt tcttccattt
    66961 cttccatcgt accgaattct gccatgattg cttgataatt actgatatca tacccattgt
    67021 catcattcgg agactcgtat actggagaaa gccaaattgc atcaatacct aaatacgcca
    67081 gataatccaa cctggaaata atcccccgca aatctccaat accatcttga tttgaatctt
    67141 gaaaacttct tggatagatt tgataaacaa ctgaatggtg ccaccatttt tgttccatga
    67201 ttttcctcct ataaatggat gtttctattt tttgcttaat catttatttt agtctctctc
    67261 actaaaaatt gtaacgcttt caaatagcta ttgcaccttg ttatcggtaa cagaataaaa
    67321 caaaaaaaca gctgaggaaa ttccctcaac tgttttttga aaggattgat ttatgcaggg
    67381 aagaatgctg atttcacaac ttctttataa acggtgttca ctcagctgtc ccttggtatt
    67441 agctcaaaaa acggcaaaat ataagaaact attttttcgt ttttttctct aatactcagg
    67501 ccagaacgct gatttcacaa cctctttatt tcacattcaa tatccacatg aatcccacaa
    67561 atacaaagaa cagaccatac atgattggag atacttcttt tcctctttta gctgctaaca
    67621 tggtaattgg atagaagata aaacctaatg caatcccatc tgaaatacta taagtcaaag
    67681 gcatgccgat caagatcaag aatgaaggga tcgcaatctc taagtttccc cattcgattt
    67741 gtcgcaaaga ttgtgccatc aatactccta ccataatcaa tgctggtgca gttacttgtg
    67801 aagtaacaac tgatagtaat ggagaaaaga acaagcctaa caagaagaaa atccctgtag
    67861 taatagctgt gatacctgaa cgtcctccaa cggcgatacc agctgatgat tctacaaatg
    67921 caccaacagg agaagtacca aataaggaac ccgcaagcat agctgttgaa tcagaagcta
    67981 aagctttccc tacacgaggc atcttgttgt ctttcatgaa acctgcttgg tttgctaatc
    68041 caactaaagt tcctgctgta tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc
    68101 acatttgaag agaattgatg tcccctacat gttttaatgc aactaaaaat gttggtttca
    68161 aacttggtgc tgcggcaaca atttcagaag gcattggaat caaacctgtg accagaccta
    68221 aaatcgttgt agctgccatc ccgatgaaga tgccacctgg tacacgccga acaagcaaga
    68281 tagcagttac gactaatcca aaaatcgtca accaagtaga accaacgttg agtgatccta
    68341 atcctactaa tgtagattca ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag
    68401 cgataaatag accgataccg ccagaaatcg catatttcaa atctgctggg atcgcatcaa
    68461 tgatcatttc ccgcagcttg aagattgtga tcaaaataaa aatcaaagaa gcaacgaata
    68521 cgccggctaa tgctgtttcc catggaatcc ccattcctaa acaaacagaa taagcaaaaa
    68581 aagcattgat cccaagagca ggtgccgttc caattggata acgggctaat acccccatta
    68641 aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa cactgctcct tcatccattc
    68701 cagaagcacc taatacagtt ggatttacaa ataagatata agccattgag ataaatgttg
    68761 taaatcctgc cagtacctca cgttttaccg tggtgttcaa tttttctatt tcgaagtagg
    68821 aaagtatttt ttccttcatc ttccatcccc ctaaatccga acattataaa taaagtccgt
    68881 taaaatttcg atttattaat aaaacaacgt gttttatcat atactaaaac aaaacataag
    68941 taaacaaaaa attcggattt taagcaaaat ccgaacgttc catctgatta aatcccttta
    69001 tctcaaagtt catgaacatt cactttggat tacaagaatt tctttattgt gttaataaag
    69061 tttcttcatt ctagtctcaa aaatcaatag tgtgatttga ttttaccatt tcgtttctgt
    69121 tcttctttga tgaattcttt tgtatcgaca ggattcagat tatttagtcc gccaaaacct
    69181 gtatcgacca tttttcccca gccttttgtc ttcttttttc ctgatttgag attctttgct
    69241 cgaagcttct cactatgttt tgctcgccga tacttcaaat aaccttctaa tcttcccatt
    69301 agatccactg tttttcctcc tttttattca atcagacgac tcagcttttc ctataacaaa
    69361 ataattgcct tcagggtcag aaaaattgaa agtcaagatg ccattgttat ctgtgatttc
    69421 tcctggttct tccatgttct cgtagatagt ttcaaattca tccgtaaaaa atactaaaga
    69481 aggcggtggt cctaatacct ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc
    69541 taattctaat tcttttgaga tgctgagtac aatactctta aattcttctg gcaattcgat
    69601 tgtttcagaa atttctgcac caattttttc tacaaagaat cggcaaatca tctctacgtc
    69661 attgacataa agcatcacac gtgttttttg tatcatcatt tgacccttct ttctatttga
    69721 ttttctttcc tgtctataaa ctgattccat tatatcacga aagcgcaaac accttgtgtt
    69781 tgatactatt caagaaaaat aaagcaatag tttctaggat tgctttcctc cacaaacttt
    69841 ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta tttcgattct tttataattc
    69901 tgcttctgat tcatctttgg ttagtgtgtg tctaacacta gttacgccaa atgtttgatc
    69961 gattttttcc ttcaacgctt cgattgcctg tgcgccacgt gccgcttgtt cagcattgat
    70021 ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc
    70081 acgccagttg agacagcgac agatcgctcc ttgttcatca tagtagatga tttcttccgg
    70141 aagtgccgga gcatcggaat ctgcacctaa aggcaggaat gattcgccac cttgggcttt
    70201 tcctaaatgc agatttcctg cgatcttttc gatatcttct ccgccgcaag gtacgccaaa
    70261 agacaaggaa atactattat agatatctac aagcggattg attgggaaaa attcccgttc
    70321 ttgatttgca cgtttcagta aagcttcaat agaagaacgt gcaccttttt tagtcttgaa
    70381 tgtggtaaat gcctgacgcc actgagcaat gacttcgttt tggctgaatg tttcttctgt
    70441 aaggtaattt cttgcttctt ttgctgcctc attcaataat tgatgatacg tttcttcact
    70501 ttcttttgtt acgtggttat cgataccatt tacggataag atttcgatgg ttgcttcagg
    70561 aaatacttcc cagaaattgg tatccactat tacttttttc atctgttcac acctcttgat
    70621 tagaataatt gcttgatgaa tagatagtct gtttggggat catctcccat ataaaaaaca
    70681 tgttcgctgt atttgataaa tcctttttta cgataaaaag cgagcgcaac atcgttgtgt
    70741 tcccaaacac cgagccaaat ggctgttttt tgcttttctt ttgctttttc aatagctgta
    70801 tctagtaaaa atgtaccgat accttgatgt ttaaattttt ttcgtacata gattcgctcg
    70861 atttctaaaa aatgatcagc catttcctca gattgactcg ttcctgtatt tagtttcagg
    70921 taacccgcag gctcttgatc gatactcact aggaaaaaat cggtttcttt atcttttagc
    70981 tcttctgtaa gtttttctag tgcataagct tgtgtcaagt aatcagctaa atgctccggt
    71041 gaattatctt ttccaaatgt atctgtaaat gtttcgatac taatttgttg tagtaagtct
    71101 acatctgaag gcgtcatttt tcgaatttcg tatttattca cggcttcttc ctttcaatag
    71161 atccttttat tgcctttttt gacaaaattc caatcttcgc taacattctt gcatacttta
    71221 tttaaaagat attccaattg ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca
    71281 ttggagtagt tgttttctcg aatgatgaaa ggatagattt ctgctccttt tttagtcgga
    71341 tagagtttct tgattttttt attttcttta tcttccaaac gttcgatcat cccattggat
    71401 tctaatttct tgattgctcg ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt
    71461 ttttcttgga tgattccagg attttcgcaa atacgtacta aataaagata ctgtcctctt
    71521 gttaaatcga cttctttgaa ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt
    71581 ccgatgtctc gaaggatttc agccatttca tcccaccttt attttgtttg taagactagt
    71641 tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa aaatcaagtt cttttttaat
    71701 aactagtaaa gtatattctt ttcttctttt acgaaaaagc tcaatcacaa tagatctggt
    71761 ttttgttttc taaaataagt aactgtttct tcgttgttcg cttttctgat agctttggca
    71821 actcttctat actaaaaaat cccatgttgg aagtttcatt attctcaata aacttggcct
    71881 cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat
    71941 ctttttgttt attcgtatca aaaactgctc gcaactgagt agtttctact gttaatcctg
    72001 tttcttcata gacttcttta cgcacatttt cttcaggaga caatcctatt tcagcaaatc
    72061 caccaggaag tgaccattct ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt
    72121 ttttgatata ggcacgaaca tctacttttg gtgttggatg tccttctcca atcgttgtga
    72181 gcgcttttat ttcttcgaaa gtttcttttc ctatttcact gatcagttcc aatgaaatcg
    72241 tccggatctc ctcaaaacgc tctttatcaa atacatcttt tccataatat agaccagctt
    72301 gagaaagtgc gagtagtctt ttgtattgag ccaacttctt gttcattctc ttcccttctt
    72361 ttttagcaaa tgtatgacta tgtaatcaag tatagaagac tggtttaatt acggcaaata
    72421 aatataatga aatctgcaat aaataaaaga atgttaaaaa actcgagaac aaaagacatt
    72481 cgacttctct ggggctgtga ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc
    72541 ccaccaaacg aaaaatatga cgagctattc ttgtctggct gttcttatta attgaggcag
    72601 gacagctttt tcacaacctc cccactaaac ggtgttctcc taccaactcc tcggtcacaa
    72661 gtcacaatat tcatcaaaca tgagaagctg tttttttgaa attgctcctt gtgactcgga
    72721 gctgaacgac aggctcacga cctctcggta aacagcgttc aaaagccaat ttttcgatat
    72781 taagccattt ctttgttact tattgtcatt gatttctttt tctaatttat ccaatagtgg
    72841 agtgtaatcc acttgagtat agtctgttac atcaaattca tatagtgttc ctaactggaa
    72901 tttgtcatat tttcggtcgg cgatgatctt tttgaatgct tctttcgtaa tatgattggt
    72961 ccctttcgtt cgatcttcca atacgtcacc atagtgataa tgatccatga tatagctcaa
    73021 atgacgatct gattcacgat cacgtttgta ccgacgttgc cataacactt caaagtctgc
    73081 tgttaggcga atcgtgatca cctgatatcc gtatctatct gctaattctt ggagtcgtgc
    73141 tttttgctta tcactaaaag gatactcgga cacaatcact ttctttcctg cttccatata
    73201 caaatcaagt gctttgtaat agaaaggata aactttcgtc ctttcttgtt ccgcccgttc
    73261 ttccaaattg ttaaacccga tggattctgc atagtattcc ttgatttcat cgagcgtgat
    73321 ggcatacata tctggaaatc gttcccgtag tttattcata agatatgttt ttcctgtacc
    73381 aggactacca gccagaagaa tgaaatactt tttcatctca tttatttcct aagttaattg
    73441 gactttccaa gttcataaac agtccaatct tatgcttgac aatttctttt actgcttcat
    73501 ttgctggaat cacattatgg cgaagagact tgttgatttc tgtttcacta aaggtttctt
    73561 tagctacttt cgtccagttg actaaaagct ccgttccaac attgaatttg cggatcccgt
    73621 tattgatcaa ttcaggataa tcttcttctt tcacacctgt tcctccatgg ataactaggg
    73681 ggacatcaac tactgcatcg atttctttca gcaagggaat gtttacttct gtttttgatt
    73741 tgaattgtcc atgattcgta ccgatagcaa tagctaatgc atccacgcct gtagcttcta
    73801 caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc attttcagcg acatggattc
    73861 cttcttctgt accgccgatt gttccaagtt ctccttctac ggaaacaccg tgcgcatggg
    73921 catattctac tactgctttt gtcttcagga tattttcttt gaaaggcaga tgggacccat
    73981 catacatgac agaagtatat ccgctgtcga ttgcttcttt gatatcatca aagtctcggg
    74041 cgtggtctaa gtgcaagact gcatcaacca tttcttcttc tgcaatagct gtcattacat
    74101 tgactaacac atgcatgccg atatatttag cggtatcgac acttgtttga ataatgatcg
    74161 gcgaacccat ttcttttgct gcgcctagca tagcaggaag catttctagg ttatgcgtat
    74221 taaaagctcc aactgtaaag ttcaactctt ctgctgtttt tgttacttct ttcaatgttg
    74281 tatacatctt ttattgccac cttatctttt tatttgtttc ctttgatcgt tacttgtttt
    74341 gcaatttgtc gcatctcatc cattttgccc atgtagtaat cgatcccttg atcatcacca
    74401 gcacgagcag catctgcacg cttctggtta tagaggctca tcagtttttt atacccatct
    74461 ccgatcgtct gtttattttc caaactcttt tctaaatatt ggatggcttg atcgttcaca
    74521 tacaattcag aaaatttaag tccaacttgt tcataaagtt tcgctaattt atgcttatct
    74581 tgggtagtgg aaatctcttg tttcaattgt gtaatctctg attgaatctt ttcggtcgct
    74641 tctggcgata gggctttttc ttcttttgcc agaacttttt tcggttcttt ttcttttttc
    74701 ttaaaaaatc caaacatcat tttccctact ttcaattaaa atgctttcaa aactgggctt
    74761 aagatccagg catataagag tgctgcggct actgtatcta catcggttgc tgtagcattg
    74821 ttgaatccca tcgaattaaa gatcgttact agcaaagcgg gcaataatgt aataaagaaa
    74881 ccatgagcaa taccgccaat gatcgctcct cggcgtccgc caactgcgtt gccaaatatc
    74941 cctgctgtcc ctccggcaaa gaaattggtt agcataccag gtaaaatcat cgctaagccg
    75001 aacatgggca aagtaaacat ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa
    75061 atgacagcat ttgggctata aggaaagaaa actggacaat ctaaggcagg gatcgcatca
    75121 ggaactaatt tcatagctat ccctcgaaat gccggcacga tttcacctaa cagtagacgt
    75181 acaccagcta gcaatacata cacacccact acgaattgga tggcttgtaa aaaggcaaac
    75241 atgatatagt tttgatctcc tgataattca gaagcgaatg gttcgcctgc aaataaaaca
    75301 gtgatgatat atagcgggac catcacgacc atgacagaca aataagtatc ttgcagaaat
    75361 tcgaaatgtg tcggcaactt gatatcttcg atcgactttt tattttcgcc tttctcacca
    75421 aaaagcttag caacaccggc ttcaaataag tacccgatcg tacaaaaatg acctaaagca
    75481 atatcatttg atccagtgat tttacgaatg atcggttgcg ctactgccgg catagcaatg
    75541 gcgaaacaag ctccgataaa accaccgacc aagatcaata cgatccctcg cagaccggca
    75601 aaatagccaa agactgtcgt catcgttgcc atccataaaa ttgcttgacc agttaaaaaa
    75661 atgtacttcc attttgtgaa acgagccaaa ataatattga atacaaaaat ggctaagaac
    75721 gtcagtgcaa tatctctacc taaacccaaa tcattcattg cttgaccatt gatagattca
    75781 atagaaggga tgatcccttg catatgaaat ccttcagtaa agattttccc gaaataagtt
    75841 aaacttccaa cgatgatgct cgaaccggca ctcaacactt gaaatcctag gagtgtcttt
    75901 aacgtgccag agatgacttg tcctgctgat ttcttctgca gtagtagacc caacatagca
    75961 atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt cgatgataaa attgataacg
    76021 cccatgtttt taccctcttt tctttttgta tttagataac tcccttatct tgcagaactg
    76081 gggtgatttt ttcttcaatt tcattttttg aaacaatatt tttcaaatag atcatcgccg
    76141 ttttaggatc aactgcgaac ttttcgaact gcgaacgaaa attttctgct gtgatgatca
    76201 catctggttt catggaaact gcggaagaaa tatcacaatg gtccagtttg gcttctactc
    76261 ctaatttgtt caaaacatcc tcagcagaca tctgtgctgc aaaactactt cctaaacctg
    76321 caccgcatac gaataaaatg ttcaatttgt tcatctttgt tctcccccgt atcatttatt
    76381 atttctcaac tactgaaaaa agcagttgtt tgaaatcttc gatatttgtg cattcaatca
    76441 atcgatccaa cttctcttcg ttgttgatca attcaattag ttctttcatg atgttcagat
    76501 gcgagtagga gtctactgct gccaaacaaa aaataatctt tactggatcc atttcaggat
    76561 taccgaaatc gattggttgc tcaatcgtag ctacgctgac tcctaattta ttgaccccat
    76621 cttctggtcg ggcatgggct agtgccatgt gtttgccaat cacgatatac ggtccgtaat
    76681 cattcactgc acgaatcatc gcttcgacat agcgctcttc gacaatgttt tctttaacca
    76741 atggtctagc agctagttca atcgattcct cccaagtagt ggctttttct ttgatcatga
    76801 tattgctgtc cgtcaaaata tcttttaaca taggctggat ctcccttttg ttgatttcta
    76861 aattgtttgt ttcgaataat ttttctaact tgccatagat gattcgatct actcggccac
    76921 cactttcttt catgacatct attatttgag tgaataactc cgtcgattca tgactgttag
    76981 gctctaaccg ctgtagttct tcatttcttt tcaaaaagtc ttctataacc aagatattcg
    77041 tctcagttaa aatcggatcg acaactaaaa gcggcttatt tcgataattc aattgaaagg
    77101 tgctgaaaac cagattgaca tccaatttat ctactaattc gatttctttt gaacttaaaa
    77161 ctgcagtaat ctcgatctga ggaaactttt ctttcagatt ctctgctaac agatttccag
    77221 tagccatccc atggttacag acaacgactg atttataaac ataattcaag tctcgcttga
    77281 ttacactggc gatcgtcgaa aagtggatga caagaaaagc tatctcgtcc tccagtatct
    77341 tgccaccaat tacttcttcg atcgtaggaa caaaatgagt gactgcgcgg tagattggga
    77401 aataatttgt tcggatgttt tctttcaatg gattcatgac ttggatatga ttcttgattc
    77461 ttacaatcag tgcagccatg tgcttataga gattttcgaa taaagcttct tcctttagat
    77521 ggaaaggtac gtgtgtctcg ttctctacaa attggatcaa ttggatcgtt agtaattgag
    77581 cttgtaccca ctcgattgaa ttggagatat ctctagcatt gaacgtctca atagtgaaac
    77641 gaagataatt tctttctact tgcgggacat tgtccaattg aaattcctcg atcatgtggt
    77701 caataaatgc agaaaagcta ctgtctttta tttctgtatt tttccaatta atggcagaga
    77761 taaattcatg tttcctgatt cgctggatcc agatcaatgt aaaaagcagg atttgttttt
    77821 tataaatatc atcttcttgt ttcaacaact tattggaata gatctcctgt atttttttga
    77881 tttccaccaa aggaaaataa tggagaaaga ttttttgagc cggtgttaat tgtgcatctt
    77941 gattcataaa gaagtcggtc attcccagat tctgattgat aaaatcatag atcatcgtac
    78001 ggatagatcg ttctgaacct ttataaacca gtccttgctt tccataactg acgatctcta
    78061 tcccataact gaccagtgtt gctcgtaacc gtctcatatc ttcatctaat gtacttttag
    78121 agatttgaaa ttcttcttct tttttgttta gatggatcgg gattcggctg taagcaacgg
    78181 ctaaaactag atcaaaaata cgttcgcgcc gattcaaaaa gccgtctttc acatctttta
    78241 tttcagtttc taatcgagct tgcttttcct tttccagctt caacaaaaaa cctttgttgc
    78301 gaattgtacg gatctccgga agctttttac tgctcaacca gatattcgct tcaagaactt
    78361 cctgacggat cgtccgctga ctgacattga actcacttgc tagatgtttt gcttttactg
    78421 gatattgggc tgtcatgatt ctgtgaagta tttcaattgt tcttgttttc atagaaatga
    78481 tctccttctg atttcaagta tatggttccg cttacataat aaaaagtaga tatttctgcc
    78541 acttgaagtg gaaagaaagt aagcaaagca aacaaaaaat aagccatatg ctttttcatc
    78601 tcctcaacga aaaagacata cgcctattat tacaaaaatt ttgcccatct atacaaaaaa
    78661 gaggttgtga aaaagctgtc ctgcatcaag taataagaac aactaaacta aaatagtgct
    78721 tcctattttt cgtttggttg ttcttattat cgcagatgca agcttttaaa caccatttag
    78781 tggagaggtt gtgacagaga tgttcagcct tgagcgataa gaagcgattt tgaaaaatcg
    78841 cttcccatat tttacaaaat cgtgccttat tgccgatagg ctatctctgg cacaccgttt
    78901 attcagaaaa aggagctgcg acaaggcttt tgtcacaact ccgttcataa atatttcctg
    78961 tcaatccagc ataatcggct gaataaaatc ggcttgccat cttttttctt tctcagaaaa
    79021 atcaaaatac catatctgag aaaaatactg tcgttgtgtc ttacttcctg agatcaatcg
    79081 cccatctttt cgatcgactg aataatcaca gcatctgaaa tcaattcgga tggcaaaact
    79141 attttcgtgg atctgtcgat aatttcctac ttcctgaata gttatttttt tagtatgatt
    79201 tcttacaccg tctgctttgt ttttttgcag aactttctga tgttcggcaa acagcttgtc
    79261 tgtataacag cttctggctt tttcaggagt ttcttcttcc caagcggatt ggatcttcat
    79321 gaatatcttt tggatttcca gaaactgtgc tttcttttct ttagccgttc ctgacatgcg
    79381 ggacaatatc tccgctttca gccttttctt ttgtctttta ttcttgacga tagaaaaagc
    79441 agaaacccct cctactgaca aaacagctaa tgcaagaaat cccgtttgct cataactaga
    79501 aggacctgta cctggaccat aatacccata agaatctctg ccgtaagaac gactgccaaa
    79561 ataactgccc ccatatgaac gaccaccaaa tgaacctctg cccgaacttc gaccgctaga
    79621 tgtacctcca ccatgaccac ccgcgacagc gaatccttgg gtcggcagag caaacaaaca
    79681 taggcccact aacaaaatga ttgctaaata attgattttt ttcattgtct tcctcgttat
    79741 cttttattac aaaaaactat ttttatgata gccttgctaa aaggaagagt caataaccag
    79801 atgcataaac tgttttttta gcaattttgt cttatttagt cattgagtaa catcggacaa
    79861 atgtatgtgt cggcagtcca taattcctct atcttcagca tgaacaagct tgcagacaag
    79921 agatctgcgc ttcctccagg agagatttgg ttttcctgac aaaagtgcaa cagtgctgta
    79981 aatggacgat ttggtaaagc actttgacta gcagcatgag caagtttttg catttccact
    80041 aacgttttct gatcactgcg atgcaatacg catgtgtcat ctagcgaact catcaaaact
    80101 aacaacatat gcatttgttt ctgtacatat gaatcattat ctagctcctt agctgcttga
    80161 attgcaagcc tgatatgcgg ataccctaat atcgcttctt catatgcacc atttacgcca
    80221 tatttttgct tcaccctatg accatgagtg gtttttttat gacgatattt tgtatcagga
    80281 aaagcagcaa gcttgctgac atcatcaagt attttcgata aagaaaaagg ctgttccata
    80341 ctgatttttt gtgcaatgac actgatcaat agccccattg cccaaatcgc tcctttatgg
    80401 gtattgatgc catttgtagc aatcagcatt gccttttccg cttttcgccc aatctcagca
    80461 attctttctc ttaactcctg atcaatagca tgatgccatg ctatttgagc catttcttca
    80521 aaaaaaggag ttagcgtctt agcagactta agaaataaag catagtccat atcggaatgt
    80581 gcacccgcat ccagtgcatc cactaatcca ggttttggac ttaatgttac ttctgcgatc
    80641 aatgcttcct ctaccaacgc agcgagttgc tgagtcactg ctttttttcg ttcgtcattt
    80701 atcatgacac tcactccttc ttcctactat tacttgctga aacctttttt gatagctacc
    80761 caaatgatat gaagtaacca gcttaaagca aatgcacaac ttgccgtcat tagataagct
    80821 acaagaatca tgactcctga aggtaactgc agccaactat ctttaaacaa taataacagt
    80881 acttggaaaa caaatacatt ggctaaatac gtacgataag cataaacaga caaccatttg
    80941 aacaatggca attttggcga atcgtttaca atcattttgg aagcgcctat gaagacagca
    81001 aagatgatga ctaaactgta taaactttgg atcgtattca agtaaggtgc attcgcaaag
    81061 ctcaagtctc ctggatgtgc gagcaatgaa cggacagaaa agaacgcgat aactaaggca
    81121 acaggtaaga aaagataacg tactctgtcc aaatattgat agactttttc gtgataaaca
    81181 aatgcggcta ccccataaat catgaaacta gcaacaaacc gatctaataa gtaccagctg
    81241 tcatgataag gcccagtcaa tacttgtgtt tgatagaaca gataccatgc gacatataaa
    81301 atcgttgaac caatcaatac tggccataca tattttttat tagcaaatac tttgtaaccg
    81361 agccaaacaa aaaacggcat gaacaattgg atctgcagca tcataacggt ataccataaa
    81421 tgaggcgctc catcacctgt gacatatttc agtaaaaagc ttgcagtatc cgtatacgga
    81481 gtttgttgtt ggactgctgg aaatagccaa agataagccg tcgtccatag aagatacgga
    81541 acgactagtt caaaaaactt ttctttcaaa aattcccaat aagacaaatg ctgacttgtt
    81601 tttaccatat tataaacaat tgcaaaaata aacattggtg ctgtgtattt aacgatgacg
    81661 taaaaggtac caagaaaagc tgtatttgtt gcgctgtggc tgtttgccaa agcaaagctc
    81721 aatatcgttt gcatcataac agccgttaca gctgatactt tctggaagtc acttacgttg
    81781 attttgattt ttttgttttc aaacatctct ttctctctct ttttctttat taccaaagtt
    81841 ctttggttaa tttaggtcct tcgactgttt tcaatagaat ttttttctct ggctctctca
    81901 aatactcttt tagtgaaaaa ccgccttgcc gagtcactac ttgggtatcg aattgctcaa
    81961 aaaaagcagg atgcgattga attacctgaa ttggcaattc aatcggtgaa tttgcgtaca
    82021 gcagcaaatc aaaatcactg gtttctttta ctgccggaat cccagtcgat aattcaaatc
    82081 ctaagctgcc gccgattccc catttacatc ctgataatat gtttctagca gcatgaaatt
    82141 gctgataaac aggataatcc atgattttct cttgtctata cgattcttgt tcgatgatgt
    82201 cccacggatg aatgacttgt ttgacggcat gttttgagat ttctgttgac catctctgat
    82261 tgcgggcagg tccacgtaaa ccaatcggta gtatttctgc gtgatccgtc gacgcacgac
    82321 gaactgttcc gaacatcttt tcagttagaa cgacccattc aggcaaggtg aacccttcta
    82381 tttcatgttt atcaaaaagg acaatatcat gtgcttgaat cattttttat gcatcccatt
    82441 cttcgtccat tttggcacgg actttatttg tttcgacacg tccgaatgtt ttggctgttt
    82501 cgtttgtgta tcgatttgtc agcatcgtgt catttgtatt gcggacatct tgaatggctt
    82561 cattgattgc ttcgatgact ttgctgacat tttgttctga agcagcttgg tcggtgatat
    82621 cttctaggaa atgatagatc gctcctagtt ttgtataatt attgatatcg taagcgatag
    82681 ctggaacttt ttcagcagct gcttcgattt cagcaaccga acgttttgtc acacgtgcag
    82741 cactttcttt agacatcgct tggacggtaa tttctttatt gtttaaagca atcaagcggc
    82801 ttgattgtaa tccatgtgct aaaaatgcac ctgaaatagc atttcctgca atcaatccaa
    82861 taactggatg tccagcttga cggagttttg cataagcagc agcactatta gccaaagcca
    82921 catggatacc gatcaattct tcgttatagc cgtatgcttg gcttggtaca tcgatcacaa
    82981 cgatgattgg ccgtttcact tttttgtctt tatcttcttc gtaaacgtga tccaaaattt
    83041 gtcccatgcg gaaaccttca actaaaccaa cttcaccgtt tcgtacacgg tacatcgggt
    83101 ttttaggatc aggtacgatt gatgtcacga tggcttcttc tatgaattta cttgatttac
    83161 cataatacac agtactaata gaagaagttg gatttttcat gccagttagt gccgcaaacc
    83221 aatcgtaacc aacacttgct gcagctggtt catttccttc tgctacttct ggtaagtcaa
    83281 ttgattttgc ttgatggtta gcaattgctt cattgtaatc gtcaatggcc attggttttg
    83341 aaagatctaa agaatcaagt aatgataaat aaaatgctga gctttcactg cgatgcgtgt
    83401 cttttcgttc gtccattgct gcgatgacag cagctttgat tgcttctaca ctatcagtca
    83461 ctaattcatc aatgattttt gattctaccc gttgacgaga tcctaatgta ttccaaatca
    83521 agtctttgtc actagaatca aattctcgaa cgcctgcttc ttgttcaata acttctggtc
    83581 cattcaaacc gactctcgct ttttttgttg cgattaaata actcattaat gctgaagtaa
    83641 tcgacatacc gccaaatgat cctacacgtc ctggaatcaa accgatgact ggtacatatt
    83701 gttttagagc aatgattaca tttccgattt ctgaaataga taacagtccg tagtttgctt
    83761 cttgcaaacg aacaccacct gtatctagta caatgactgg aaagatctca tttccttttt
    83821 tattttcttc taacacatgt tcaagtgcag caacgatttt tgcaccggaa acttcaccga
    83881 taccgccacc ttggaatttt ccttccatag cgattactac tgcttgtttg ccattgatcg
    83941 tgccacggct gacaacgatc ccgtcatcac tttcagggac gattccttgt gctggtaaac
    84001 ttggcgcgat gatttgatcg aatggatcaa tcaattcacg tccattgtca agtagagcgt
    84061 tcactcgttc tctggcgttt aattctacaa aactattttt catctgaaag ttcctccatt
    84121 gcttgcgtca atcgtaggta cacaactcct ggtgtagcac cgaagtcatt gatcacgata
    84181 tctgctgaaa ttggataacg atcaaaaaat ctcgttaata cattgttcca tacttcgccg
    84241 aagccatcgc ttcctgtaca aatttttaca gtagattctg ttttttctgt tggaaacatc
    84301 aagatttcca aatctcctga accgaccact cctacatgga ttttcttatt gatcggctgt
    84361 ttcgttggat atttataagc taattgttcc atggatctct cccctttttg taaaacagag
    84421 catacacgtt ctgcttattc gtctttcttc ttccatctgg gtaaatggcg acaaaaggtt
    84481 tttctcgcca attttatgac caagttctga atttcattgg tggttcatac aggccatctg
    84541 accattcgat caaatcatcg atggtttgtg cagctaataa tgaacgtttc gcttcactac
    84601 gtttgatgcc tagatcttct ggtaatttca cgatgccggc tgcacgcaag ctatctagtg
    84661 attctttcgt gctagtcatt ccaagaggtg taacaccgcc aattgctgca attgctgctt
    84721 gtcgttcttc ttttgttttt gctttgtaca aataagcaat tccttcttct gtcacgatat
    84781 gtgtagtatc ttcgctataa atcatcactg gtgcattggc taagcctgct tgtttttgaa
    84841 cagctagtgc atctaattct tcgacaaaga ctggtttttt atttgaacca tatgtttcaa
    84901 ccatttgaac aactaatttt tggcctttcc ctaatggatc atcattgtcg cgaagtgatt
    84961 gccatgctgg tgtagaatga cgacgaccgc caggattatg tcccatgtta ggcgcaccgc
    85021 caaaaccaga taaacgtcct tttgttacgg tagaagagtt tccttcgtaa tctaattgca
    85081 aggttgaacc gataaacata tcgatggcat attgtcctgc agcttgtgat aatgttcggt
    85141 tactgcgcat cgtgccgtct ttaccgacaa aaaagacatc tgggcgagca gcaatatatt
    85201 tttccatacc aacttcgcca ccaaaactat ggatgctttc aacccaacct gattcgatgg
    85261 ctggaatcat cgttggatgt gggttcaata cccagttctt cgcgattttc ccttttaacc
    85321 ctagtgattc accataagtt ggcagtaaca attcgatagc tgctgtattg aatccaatac
    85381 cgtgattcaa agattgtacg ccgtgttctg cgtagattcc tttgattgcc atcattgcca
    85441 ttagaatttg caattctgtg atgttttgag gatctcgtgt gaataaagct tctaattgat
    85501 aaggtttatc tgctttcacg acaacgtcta cccagtcacc agggatatct acacgtgg
//
LOCUS       DAYLZO010000002        69370 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.002, whole genome shotgun sequence.
ACCESSION   DAYLZO010000002 DAYLZO010000000
VERSION     DAYLZO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.002"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..571
                     /locus_tag="ACGVJG_000076"
     CDS             <1..571
                     /locus_tag="ACGVJG_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFJ6556069.1"
                     /translation="ETVLIHAGASGVGTAAIQLARQLSHAKIIVTAGSEEKLAFCKEL
                     GADELINYKKQDFQEEVRKITEGRGVDVILDFIGASYWEKNLASIAVDGRWVLIGMLG
                     GTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELAQTFMQKAGPFLENGKIRPIIDR
                     TFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(974..2488)
                     /locus_tag="ACGVJG_000077"
     CDS             complement(974..2488)
                     /locus_tag="ACGVJG_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFJ6556070.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFNEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            2626..3432
                     /locus_tag="ACGVJG_000078"
     CDS             2626..3432
                     /locus_tag="ACGVJG_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556071.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(3607..4176)
                     /locus_tag="ACGVJG_000079"
     CDS             complement(3607..4176)
                     /locus_tag="ACGVJG_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6556072.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4259..6319)
                     /locus_tag="ACGVJG_000080"
     CDS             complement(4259..6319)
                     /locus_tag="ACGVJG_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFJ6556073.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(6596..8074)
                     /locus_tag="ACGVJG_000081"
     CDS             complement(6596..8074)
                     /locus_tag="ACGVJG_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556074.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(8372..9307)
                     /gene="murB"
                     /locus_tag="ACGVJG_000082"
     CDS             complement(8372..9307)
                     /gene="murB"
                     /locus_tag="ACGVJG_000082"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFJ6556075.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(9380..10300)
                     /locus_tag="ACGVJG_000083"
     CDS             complement(9380..10300)
                     /locus_tag="ACGVJG_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFJ6556076.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            10413..11165
                     /locus_tag="ACGVJG_000084"
     CDS             10413..11165
                     /locus_tag="ACGVJG_000084"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFJ6556077.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            12029..12562
                     /locus_tag="ACGVJG_000085"
     CDS             12029..12562
                     /locus_tag="ACGVJG_000085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFJ6556078.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(13052..13576)
                     /locus_tag="ACGVJG_000086"
     CDS             complement(13052..13576)
                     /locus_tag="ACGVJG_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFJ6556079.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(13569..14042)
                     /gene="tsaE"
                     /locus_tag="ACGVJG_000087"
     CDS             complement(13569..14042)
                     /gene="tsaE"
                     /locus_tag="ACGVJG_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFJ6556080.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(14165..15145)
                     /gene="pta"
                     /locus_tag="ACGVJG_000088"
     CDS             complement(14165..15145)
                     /gene="pta"
                     /locus_tag="ACGVJG_000088"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFJ6556081.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(15298..15978)
                     /locus_tag="ACGVJG_000089"
     CDS             complement(15298..15978)
                     /locus_tag="ACGVJG_000089"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFJ6556082.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            16104..16973
                     /locus_tag="ACGVJG_000090"
     CDS             16104..16973
                     /locus_tag="ACGVJG_000090"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFJ6556083.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            16999..17514
                     /locus_tag="ACGVJG_000091"
     CDS             16999..17514
                     /locus_tag="ACGVJG_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFJ6556084.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(17561..18124)
                     /locus_tag="ACGVJG_000092"
     CDS             complement(17561..18124)
                     /locus_tag="ACGVJG_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6556085.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(18179..18358)
                     /locus_tag="ACGVJG_000093"
     CDS             complement(18179..18358)
                     /locus_tag="ACGVJG_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556086.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(18435..18944)
                     /locus_tag="ACGVJG_000094"
     CDS             complement(18435..18944)
                     /locus_tag="ACGVJG_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556087.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(19040..19419)
                     /locus_tag="ACGVJG_000095"
                     /pseudo
     CDS             complement(19040..19419)
                     /locus_tag="ACGVJG_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            19749..20498
                     /gene="pnuC"
                     /locus_tag="ACGVJG_000096"
     CDS             19749..20498
                     /gene="pnuC"
                     /locus_tag="ACGVJG_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFJ6556088.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(20682..21959)
                     /locus_tag="ACGVJG_000097"
     CDS             complement(20682..21959)
                     /locus_tag="ACGVJG_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556089.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(22038..22682)
                     /gene="pcp"
                     /locus_tag="ACGVJG_000098"
     CDS             complement(22038..22682)
                     /gene="pcp"
                     /locus_tag="ACGVJG_000098"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFJ6556090.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(22881..23825)
                     /locus_tag="ACGVJG_000099"
     CDS             complement(22881..23825)
                     /locus_tag="ACGVJG_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFJ6556091.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(23827..24513)
                     /locus_tag="ACGVJG_000100"
     CDS             complement(23827..24513)
                     /locus_tag="ACGVJG_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFJ6556092.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            24987..25307
                     /locus_tag="ACGVJG_000101"
     CDS             24987..25307
                     /locus_tag="ACGVJG_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFJ6556093.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            25461..26366
                     /locus_tag="ACGVJG_000102"
     CDS             25461..26366
                     /locus_tag="ACGVJG_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556094.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            26363..27136
                     /locus_tag="ACGVJG_000103"
     CDS             26363..27136
                     /locus_tag="ACGVJG_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFJ6556095.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(27317..28741)
                     /locus_tag="ACGVJG_000104"
     CDS             complement(27317..28741)
                     /locus_tag="ACGVJG_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFJ6556096.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            28952..29398
                     /gene="nrdI"
                     /locus_tag="ACGVJG_000105"
     CDS             28952..29398
                     /gene="nrdI"
                     /locus_tag="ACGVJG_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFJ6556097.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(29463..31445)
                     /locus_tag="ACGVJG_000106"
     CDS             complement(29463..31445)
                     /locus_tag="ACGVJG_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFJ6556098.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(31459..32355)
                     /gene="murQ"
                     /locus_tag="ACGVJG_000107"
     CDS             complement(31459..32355)
                     /gene="murQ"
                     /locus_tag="ACGVJG_000107"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFJ6556099.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(32358..33419)
                     /locus_tag="ACGVJG_000108"
     CDS             complement(32358..33419)
                     /locus_tag="ACGVJG_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFJ6556100.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(33630..34157)
                     /locus_tag="ACGVJG_000109"
     CDS             complement(33630..34157)
                     /locus_tag="ACGVJG_000109"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFJ6556101.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(34224..35726)
                     /gene="eat(A)"
                     /locus_tag="ACGVJG_000110"
     CDS             complement(34224..35726)
                     /gene="eat(A)"
                     /locus_tag="ACGVJG_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFJ6556102.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(35984..37105)
                     /locus_tag="ACGVJG_000111"
     CDS             complement(35984..37105)
                     /locus_tag="ACGVJG_000111"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFJ6556103.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            37231..38172
                     /locus_tag="ACGVJG_000112"
     CDS             37231..38172
                     /locus_tag="ACGVJG_000112"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFJ6556104.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(38322..39218)
                     /locus_tag="ACGVJG_000113"
     CDS             complement(38322..39218)
                     /locus_tag="ACGVJG_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFJ6556105.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            39487..40827
                     /locus_tag="ACGVJG_000114"
     CDS             39487..40827
                     /locus_tag="ACGVJG_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFJ6556106.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            41045..41488
                     /locus_tag="ACGVJG_000115"
     CDS             41045..41488
                     /locus_tag="ACGVJG_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFJ6556107.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(41992..43410)
                     /gene="nhaC"
                     /locus_tag="ACGVJG_000116"
     CDS             complement(41992..43410)
                     /gene="nhaC"
                     /locus_tag="ACGVJG_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFJ6556108.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(43813..45036)
                     /locus_tag="ACGVJG_000117"
     CDS             complement(43813..45036)
                     /locus_tag="ACGVJG_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFJ6556109.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(45067..45906)
                     /locus_tag="ACGVJG_000118"
     CDS             complement(45067..45906)
                     /locus_tag="ACGVJG_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFJ6556110.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(45923..46603)
                     /locus_tag="ACGVJG_000119"
     CDS             complement(45923..46603)
                     /locus_tag="ACGVJG_000119"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFJ6556111.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(46605..47678)
                     /locus_tag="ACGVJG_000120"
     CDS             complement(46605..47678)
                     /locus_tag="ACGVJG_000120"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556112.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(47734..47972)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(48015..48248)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(48325..48996)
                     /locus_tag="ACGVJG_000121"
     CDS             complement(48325..48996)
                     /locus_tag="ACGVJG_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFJ6556113.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(49083..51731)
                     /gene="mgtA"
                     /locus_tag="ACGVJG_000122"
     CDS             complement(49083..51731)
                     /gene="mgtA"
                     /locus_tag="ACGVJG_000122"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFJ6556114.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(52073..52247)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            52420..52857
                     /gene="ndk"
                     /locus_tag="ACGVJG_000123"
     CDS             52420..52857
                     /gene="ndk"
                     /locus_tag="ACGVJG_000123"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFJ6556115.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(52879..53745)
                     /locus_tag="ACGVJG_000124"
     CDS             complement(52879..53745)
                     /locus_tag="ACGVJG_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFJ6556116.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(53905..55998)
                     /locus_tag="ACGVJG_000125"
     CDS             complement(53905..55998)
                     /locus_tag="ACGVJG_000125"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFJ6556117.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(56747..57370)
                     /locus_tag="ACGVJG_000126"
     CDS             complement(56747..57370)
                     /locus_tag="ACGVJG_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556118.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(57529..58926)
                     /locus_tag="ACGVJG_000127"
     CDS             complement(57529..58926)
                     /locus_tag="ACGVJG_000127"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFJ6556119.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(58958..59929)
                     /locus_tag="ACGVJG_000128"
     CDS             complement(58958..59929)
                     /locus_tag="ACGVJG_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6556120.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            60303..60728
                     /locus_tag="ACGVJG_000129"
     CDS             60303..60728
                     /locus_tag="ACGVJG_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556121.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(61216..61917)
                     /locus_tag="ACGVJG_000130"
     CDS             complement(61216..61917)
                     /locus_tag="ACGVJG_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFJ6556122.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(61933..62373)
                     /locus_tag="ACGVJG_000131"
     CDS             complement(61933..62373)
                     /locus_tag="ACGVJG_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFJ6556123.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(62373..62948)
                     /locus_tag="ACGVJG_000132"
     CDS             complement(62373..62948)
                     /locus_tag="ACGVJG_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFJ6556124.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            63189..63398
                     /locus_tag="ACGVJG_000133"
     CDS             63189..63398
                     /locus_tag="ACGVJG_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFJ6556125.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(63796..64308)
                     /locus_tag="ACGVJG_000134"
                     /pseudo
     CDS             complement(63796..64308)
                     /locus_tag="ACGVJG_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(64434..65135)
                     /locus_tag="ACGVJG_000135"
     CDS             complement(64434..65135)
                     /locus_tag="ACGVJG_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6556126.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(65243..66686)
                     /locus_tag="ACGVJG_000136"
                     /pseudo
     CDS             complement(65243..66686)
                     /locus_tag="ACGVJG_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(66700..68565)
                     /locus_tag="ACGVJG_000137"
     CDS             complement(66700..68565)
                     /locus_tag="ACGVJG_000137"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFJ6556127.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(68686..>69370)
                     /locus_tag="ACGVJG_000138"
     CDS             complement(68686..>69370)
                     /locus_tag="ACGVJG_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFJ6556128.1"
                     /translation="NQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDH
                     LHYAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAF
                     HFVNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRY
                     LKGEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRV
                     VSEKKSIQS"
BASE COUNT        19988 a        14408 c        12054 g        22920 t
ORIGIN      
        1 cgaaactgtc ttgatccatg caggagcaag tggtgtggga acagcagcga tccaattagc
       61 tagacagctt tctcacgcga aaatcatcgt tactgcagga tcagaagaaa aactcgcctt
      121 ttgtaaagaa cttggtgcag atgaattgat caactataaa aaacaagact ttcaagaaga
      181 ggtacgaaaa atcactgaag gtcggggagt cgatgttatt ttggatttca tcggcgcttc
      241 ttactgggaa aagaatttag cttctatagc cgttgatgga cgttgggtct tgattggtat
      301 gctaggtggg accatcgtgc ctgagataga ccttggtgta ttgattggga aacgtgtcca
      361 actgcttggt acattattga cacctagaag tgatcagtat aaagcagagc tcgcacaaac
      421 ttttatgcaa aaagctgggc cttttctaga aaatggcaag attcggccaa tcattgaccg
      481 tacattccaa ttcagcgcag tcaaacaagc gcatgaatat atggaagcaa acaaaaacat
      541 cggaaaaatc attttgaaga taactgaata aaatcatgct gatcaaaaaa gaggctggga
      601 cagaagcgtt taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt
      661 gtgaatttca gcttatttcc gaaggaattt cttctgcttc cgccgtttat acgtgtttag
      721 agcgtgagac aaaagtgttc tttacttttg tcccacgctc tttttccgaa taaacggtgt
      781 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag aagctgtttt
      841 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct tcactaaaca
      901 gtgttcaatc agctgaccct cggtattagc tcaaaacgct gatttcacaa cctcttttta
      961 tttacgttct atttcattca ttttccgaag caaatcgagc tgcttgtatg cccgcttgtc
     1021 gcccaaaaat aatgatctcc gcaactgaat ttccaccgat ccggttgttt ccatgtaagc
     1081 cacctgtgac ttcaccagca gcatataacc ccctaattgg ctggttttct ttatctaata
     1141 cttcagtgtt cgtattgatt ttcacaccgc ccatcgtgta atggattcct ggtgcgattt
     1201 tgatcgcata ataattcgga gacgataagt catgttccat ggctgtggca cggttgaatt
     1261 gagcatcttt tttctccgca acatccgtat tccatccatc aactgttttt gctagtgttt
     1321 cttttggtaa atcgattttt tctgctaatt cttccattgt ctttccagtt gtgacatatc
     1381 ctttttggtc ataaaattca atagctgtgg cacgatcacg cacgccttgg tcaaaaatca
     1441 aataagctga tttttcaggc aatgctgtaa ttgctgcaga tactttatcc cttgtatcca
     1501 tttcattgac aaatcttttc ccctcttgat cgaccaagat tgctccttct ccgcgaacaa
     1561 cctcaccaat caagaccccc tcatcctgct gcacagttgg atgaatttgt attttatcca
     1621 tatcaacggt ttgtccacct aatttttcaa tcatggtgat accatctcca gttgatcctt
     1681 cttgatttgt cgtaatataa tcagctaaat cgggacgata tttttcatta aattctttgc
     1741 tggcaccaaa accgcctgtg gtaactacga ttgcttttcc ggtgacctct tttggttctt
     1801 ctccttgaac atgtacagtc acttcattga ccgtttcccc atttttctta atgtctgtca
     1861 catctgcgtt gacaaaaata gggatttccc gctcatggac gttattcaat aacccctcca
     1921 ctaagtatcc accaatagcc gagccatcag acggacgatg tgtcctcttt tcactcatcc
     1981 ctccagtgat cgttaagttg tccagtttga taccgttttg atctagccaa tcgaccgcat
     2041 cagcagagtg atcgacaaag tatcgaagca tttctttatc attcgttcct ccaccacctt
     2101 ttagcgtttc ttcatagaag gcatcattcg agtctttgat tccttcttct ttttgaaatt
     2161 ttgtttctga agcattcata ccgcttgatg atttagatgt gtttcctccg gcaacaggca
     2221 tcttttccaa aattactgga ttcaacccag catcttttgc ttcgatagct gctgccatcc
     2281 ctgcaccacc agctccaata atcacgacat cataagattc tttcatttct tttggatctg
     2341 tatatacttg ttcagaagcg cctgatgata cttcggcaga ttccttcgta ctagaacttg
     2401 tctgcgctgt atttttctga tctgcgccgc atgcgctcat agcaagaagt gcaacggctg
     2461 ttaataaacc agcaactaat tttttcattt gtatccccct tttctcctta tactatatta
     2521 agtgattttt ttcacaatat caactctgtt tttctttaag tcatatagct ttggtaaaca
     2581 cacgcttctt agtataatta aggatataca tgaaggagtt attttatgaa tcatttgaaa
     2641 acctttttta cgaacagtac aaaaactgca ggtagaatca caaacggaat ctatatttta
     2701 ttacgtatca tcgtcgttat tatgctagtg gatcaggtct ggaaccaaca ctggggaaat
     2761 acaatgcttt tattactgac attaatttta tttgaaatcc cttattttct ggaaaagata
     2821 ttaaagatac agattccaaa tatgctagaa atcattttgt tatcttttat ttttagttca
     2881 acagttttag gagagttaag tgatttttat ggctattttt caatgtggga tacttttcta
     2941 catgcattca gcggattttt agctgggggt gtcggctttt cccttgttta tttgctcaat
     3001 aaaaatacaa aaaccatgaa cttgactcct ttattactag cagttgtttc cttctgtttt
     3061 tcgatgacag ttggtgttat gtgggagttt gtggaatttt cagcagataa attactgcaa
     3121 ttcgatatgc aaaaagatac gatcatccag caaatcaata gtgtggagtt aagtaaagac
     3181 ggaaacactg tctatcatgt tccagatatc actcggacaa tcattgaaag tcaaaatgca
     3241 gatggtaaaa cagataaaac aatcatccaa aatggttact tagacgtggg aatcatggat
     3301 actatgaagg atttatttgt taattttatt ggagcagttg ttttcagtgt atttggttac
     3361 ctctatagtc gaaacgatta ccaaaaattt acgttcatca agaattttat tcctaaaaaa
     3421 atcaaacgat aaaacatgct aaaaaacaag agaaggggca gtgacaatac ttttgtcact
     3481 gccccttctt ttttgaatgt atggcatgcc attagttatc cgatagtatt aactcaaaaa
     3541 agtaagatat gaggaatgac tatttcgttt tttatcccaa tactgaggac ttatttcacc
     3601 atttcgttat ttatctaatt tacttaaaac ctgcataact ttttcataat ctggattagg
     3661 tgtgccgtct agattttcag caataaaagt aaaacccatt tttttataaa gagcaatcgc
     3721 ctgatggctc catgtctgtg tgtgaagata gattggtccg tccgtatgga gggtaggaaa
     3781 ttcttgcaag actctggcag tcaatgcttt tgcgagccct tttccttggt aatctggttt
     3841 cactgctaac caatgaaaaa ccggatacac ttgctctctt gatttttttc gccaagcggt
     3901 acaggttgct actttttctc ctttttttgt ctctacaaaa aacatccttc tttcaagttc
     3961 ttcaggataa ggagcaaagt tttgttgaaa atactcaagt gcttcatctg tcgtttgaaa
     4021 ttccccaacg gaagtctcaa tctccgccca agcagattca tcttcttttt gataaaatcg
     4081 aaattcaaaa ccttccgcta aaggcgtgat tggaacattt tgttgaagtg gtcttgtcat
     4141 ccagatttct gcgtaaggta acgttttatc taacattttt ctatccctcc tcgtctccat
     4201 tatgccaaat aacatatgac tcagctactt atttgactat aacagataga gatataaatc
     4261 agaaccagtc atctgcgtca aattcatttc gattagcgta tacccatgtt cctgccatac
     4321 ctcgcatgaa atctcctaaa gcagaagaag gaatacctcc accaggtgcg ggtgttcccg
     4381 gactaggaag tggtacttgc ggctgcagat gctcatcggt ggaatcaata gttgacaagc
     4441 gttttttctt cgtttccttc tggtcatctg attccttctg atcttccgac tgtttagcaa
     4501 caaattcttt ttctgattgt tgttttattt taggctctaa aatttcattt tccaatccag
     4561 aaacttgtgg ggccaaatga gcttcttgcc ttatcgtttt attagattct gagatttttc
     4621 tagatattct tgctaactct aggcttcttg tttgttttac tttccctgtt ggagaaactg
     4681 catgagccat ttgttgtttt ttttctgaca agctttcttc ttgtcttttt tgttcttttt
     4741 gatctatctg tttgtttaat ggtaaaacat gtaatagtga ctgagcatgg agttcttgga
     4801 gtgaatacgg gtcatgataa ctatacataa tcgtaaaatc acctgtttgt ttggtcaaat
     4861 aaccatcctc caatggtact tcattgattg taaccgatac atcctttaac ggtacgggtt
     4921 tacctaagga atttgttgct gattggatca actcttcttt gtttattttt tcgtttggat
     4981 gaacaataag atcatgacct tctaatgaac gattgtctgg tatataaaca taagtaaagg
     5041 taatttcccc tcctgattgc ttcctattat tcatcagttc tgagtgaagt acttcaagga
     5101 tctccgacca gtgacttttt ctgtctaatc cgtttgcttg catccaatca gaaatagaag
     5161 tgactttccc attttgttca taagtcgata aatctatatt tggcactcga tcagaaaatt
     5221 cagcaggtag gcgcaaatca ggaatacgat ttacactact catatgtgtt tctgcttgaa
     5281 gttctttatt tgtttgacta tctataaaat gagtgatgac agtcgatgaa gcagcagatg
     5341 cttctacata aactacagct gttgtttctg ccacatttga tgttttcgag tctatataag
     5401 ttaatacaac gggatattct cccggagttg tagtatctac tgtcccgttc attttgattt
     5461 cttctttatt tcctgaagtt ccatcgcttt ttgttacata ttcaacaaga tctttagggg
     5521 cgtaactctc tcctgttctt aagtgaagat cttttccttt gatagctgtt tgatcagcac
     5581 gaacatggat cacagcagat gcagtcattg tttttaaagc aattggatca aaataggaca
     5641 aggaaatagt agatgttcct gtattagtcg tatccacgat attcccattg tttgaaatta
     5701 ctttggacat atctccttgt gttccatctg cgttcttcag cctttctacc agatcagata
     5761 tcgaaacttt tgtccctgcc agaacatcga ttttctttct ttcaaactgt gaataatctt
     5821 tttcatatac ataagaaact tgtgcgcttc caccgtcctt tatatctgtc aaagcatgat
     5881 tcaaaatatc agtgatctgt tttaactgat caccttttgt aggatcagtc atatcggaaa
     5941 agatagaaag cggcacactg cttccggttt ccgatgtcca taatgttttg ttaggaatta
     6001 accagtagtg gtttatcgtt ggaggataac tgactttatt ataggaagtc aatgcaggaa
     6061 gtagctgttt tccatttgca tcagtgaaag tatgagtaat agtgataggg gaatcagtgg
     6121 tctcagaaac agaggtacgt acgaccttga tggtttgtgt cgttgcctga acgggtacat
     6181 gcatatagac acctacagat aaactgatca gcatcgtgcg catcataaac ttaacgtagt
     6241 gtttttcaga acacgaccgt tccttatcta aacgacaagc gtgtttattt agttgttttg
     6301 aacgtctttt tgaacacatt tattcttagt cctccctact cttgtctaaa catgaagtag
     6361 taattttcat aagatgattt cattatataa ataaaatcaa aagaagatgg actattagga
     6421 gagtattttt tttgattttt tattgtttca agaaaatcgg aataataact tatgtacttt
     6481 ttaattttgt taagtaatat atctagttat atctagttat atctagttac cttttctttg
     6541 aaaaaaacat aaatcagcta ataaatatgt gagacaaagc tgccatccat aaatactagc
     6601 tgattttttc agttaaatgt ctattttttt ctctgatgat ggtatttcca aaatattccc
     6661 agtcatctgg agtcggtgga tttttccaaa ttatctgttc tttattaaat ttttcaagta
     6721 aacaatcaga taaataaata ttcgtacgag aagtgattcg actctcaatc acaatattgt
     6781 aatttttaaa tcctttcact tgagaaataa tgtaatcggt ataagcagat ccttgcgaaa
     6841 aatcaataca gacatgaatc ggtgcttcaa aatatgatac cggacaaata ccgaccaata
     6901 aaaagagata atcataaaat aagcgaatca tgaaacgttc atcttttccc aagaaatagc
     6961 gtttattctc ttgtatgacc ttttggattg ctgtactgaa tattggatac gtctcgaaga
     7021 aaaactgtag ctgcttttta gacgtaaagc tagtcatctc aaagtcgaat atccaatttt
     7081 ttcgattgac atgcatcata cccatacgaa gttcttttcg tatatatagt ctttcttctt
     7141 cagagatttc aatcgaaaat ggtaattgat caatcaattc cactgacatt ttttgactaa
     7201 tcatgtggat atcatgaaaa tgagcttccg caagtccaaa tgcgccatct aattcaaatt
     7261 cttcttccgt gatcaaaaac aacatgatat aagaaatctc atcaatgatc tgctctttag
     7321 ggatatgaag aaaagaaata tgtgataaat ccttgatgat cttcattcct tctgagctct
     7381 taaagtattc atctgaaaag aaacgttttc cgattggttg attcccatag gccctcaaca
     7441 aactaatagt ggttagcaaa ttgaatttga ttcgttttgt tgcagacagt tttaaattga
     7501 acagatattc aaaatattgc acgatactct ttgcttcatt catgacgctt tctctaaatg
     7561 gcatagaaaa accattaaag accccgaaat ataccccaaa taatacgttc cgtatgttta
     7621 tttcttcccc aactaatatg tttttcttta actttatttg atgctgttca agaaaagaga
     7681 ctaattctct tctttttaca taggctcttg atatgcttaa aaaatgattc tctgcaaatt
     7741 tttcgatcgt tacttttgaa tccacgatct ccgttaacag tcgagcaatt tttgatcgtt
     7801 gaaagtagat tcttcttaaa tttttgatat ccgtcaaatt cagacgttcg acatggatct
     7861 ctgcttcttc tgaaatttgg atctctggct ttgttttgaa ttcagtaaat tctttattta
     7921 attctatgat ataatttctt gttttaaatt tagacaaacc taatgtttca tataacttag
     7981 gcagcactac ataattcttt ttctcaaatt ctaaatatct agctagcagc agtttttctc
     8041 tatcttcctc ttctataagg taatccaact tcatatccta cacctcaaaa aatgcctcat
     8101 accaattact tgatatgcag atgaaagttc atcttttcac ctatactgat tttatgatgg
     8161 attatgcttg ttttttcatc agttattctc attttttatc ttttttttga gaatattgca
     8221 aaaaatggaa aaatttatga ggaaatattc tatatttttt gttttattgt gttaaaatgt
     8281 acaagatttg cgccatttgc gtaaacaacc acaaaaaaga gcaggacggc caccgtcctg
     8341 ctcttttact tattttaagt tccattactt attaattggc taaacttctg cttgtttctg
     8401 atattgcttg tttttctcca atgatccgca cttctgtttc taaagacaca tcaaattttt
     8461 ctttgattac ttcttggatg tgcgcaatca attcaatata atctgtcgct gtcgcatgat
     8521 cgatattgac gatgaatcca gcatgttttt cagagacttg tgcaccgccc catttcaggc
     8581 cttgcaatcc tgcttgttgg atcagctgac ctgtatagtg tcctactggg cgcttaaaga
     8641 cacttccgca tgaagggtat tctaatggct gttttgattg acgcaattcg gtcaactcat
     8701 ccattcttgc tttgattgct tcataatccc ctgtttgcaa actgaatcga gcttccaata
     8761 cgatagcacg taattcttgg actttactgt gacgataaga aaataccatt tcgtcttttg
     8821 tcaaagtttt caatgttcca tcttcaagca aaagatccac ttcagcaaaa acatctttga
     8881 tctctccgcc atacgcacct gcattcatat aaaccgctcc accgacactt cccgggatgc
     8941 cgcaagcaaa ttcaaatcca gtcaatgatt ctgccaaagc tgcataagtc gtatcaatca
     9001 gtgaggcacc tgcttccgca atgaccatcg ttcctttgac atgaatctgt ttcatttcag
     9061 tcagcatgat gactacaccg cgaatcccgc catcttgaac gatcagattg cttgcatttc
     9121 ctaataccat ccaaggtata tcatggattc ggcaataatc gatgatttgc tttacttctt
     9181 cttgtttttt cgggaatgca aggacatcag ctggtccacc tgttttcgta aacgtatagt
     9241 tcatcaatgg ttcatcaaac aatatagtaa tcgtggctaa gctttcaact atttcttctt
     9301 tattcactga ggattttcct ttcttttctc aaacgtcctc attattctat cacgcttaat
     9361 acaatcttgc cagtcttgtt caaagttgat gacggtagtg atctttaagc acttcctcac
     9421 ataattcatg acttcttaca acttcttttt gtttaagcaa ttctgtttcc gatttaccta
     9481 aacggatctg agaaaagaag gcctctacca ttggctcaaa cccgcgtttg actagcgtgt
     9541 ttgtccagtc gccaaattct ttcacttttt ttccatcagc atcttgaatg acaagctggg
     9601 tcagatcgtt tacttcataa gtaccatttt tacttgttac ttgatatttt tctatattag
     9661 cgccacttgc taaatccatc gttaaaatag ccgtttgatt ttgtgtttct aattgtaaaa
     9721 tggcatattc caaaaattcc tttgtttctc ttatctggta ttttgttctc aaaatgggtt
     9781 catctaataa atacacagct gtatcaataa gatgtaaaaa caaatcataa aggacgaact
     9841 ctgtcgtttc ttttgccgct attcgatttt tttgcaggtg gatcaaccgc ttatctggga
     9901 tacgtttcaa ttcttctacc ataggagcaa agcgccggtt gaaaccgatc atcaaaatta
     9961 tttggttctt atcagctaat tcttgcaatt cacgaacttc atttagatgg acactcagtg
    10021 gtttgtcaat ataaacatgg ataccatttt ctagacattt tttggctaat tcataatgta
    10081 cttttgttgc tgcgtggatc atacatgctt cgattttttc ttctaaaagc tcatcgatcg
    10141 tttcatatag atgttggaac ccgtactctt tttgaattct ttcttttgtt tctttatttc
    10201 ttgttgcaaa ataaaaatca agtgttcctt gttttttcgc ataagttggt aaatatgctt
    10261 tttctgcgat attccctaca ccgattacac caatcttcat ctactttcct cctggatcat
    10321 ttagttactt tcatcttaca ccatttttta ctaaaatgag gtacaatagt atcgcaaagg
    10381 taaggaaatt aattcgaagg agtgtgcttt tattgaaatt tatctcttgg aacgtaaacg
    10441 ggcttcgtgc catcgtaaat aaaaattttt tagaagtttt tcatgagtta gacgccgatt
    10501 ttttctgtct gcaggaaacg aaactccaag caggacaaat tgatttagat cttccaggtt
    10561 attatcaata ttggaattac gcggaacgaa aaggctattc cggcacagcg atttttgcta
    10621 aaaaaccagc attgaatgcc acttacggaa tgggtatcga tatccatgat acagaaggcc
    10681 gtctgattac tttggaatat tccgatttct ttcttgtaac ctgttacacg ccaaattctc
    10741 aaagcgaatt aaaacgttta gattaccgat tagaatggga agaagccttt tataattatt
    10801 tggaaaatct aaaaaaacaa aaaccagtca tcgtgtgtgg ggatctaaat gtcgctcatc
    10861 aaaagattga tttgaaaaac tggaaaacga atcaaaaaaa tgctggattc actccagaag
    10921 aaagagcggc tctttctcga ctgttggata acgggtttat cgatacattc cgttattttt
    10981 atccaaccca ggaaggtgtc tactcttggt ggaactaccg attcaattca aggaaaaaca
    11041 atgctggttg gcggatcgat tactttttga cgagcaaaga tttagagcca ggactagctg
    11101 atgcaaagat ccatacggac attatgggca gtgatcattg tccagtagaa ttagatttga
    11161 aataaagagg ttgtgaaaaa gtcgtttagt ttcgagtatt aagaaaaatt ttgtgaaaat
    11221 ggctttccac attttgcata aatttggcat aataccgaga aactggcttt tgaacactgt
    11281 ttatcgagag attgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa
    11341 tagttcctca tatttttcgt ttggttgggc ttattaccgc agatacaagc ttttgaacac
    11401 cgtttattaa gaggttgtga aaaagctgca ctgcatcaag taataagaac agccaaacaa
    11461 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
    11521 caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa
    11581 caaaaatagt tcctcatatt tttcgtttgg ttggacttat taccgcagat gcaagctttt
    11641 gaacaccgtt tattgagaag ttgtgaaatc agcgtcctga cctgagtatt agagaaacaa
    11701 atggcaaaat agttcctcat attttgctat tttttgagct aataccgagg gtcagctgat
    11761 tgaacaccgt ttagtggaga agttgtgaaa aagtcgccta ctttcgagtc gtaagaaatt
    11821 tttttgaaaa tggttcctca caactttgca taaatttgac ttaataccga gaagtcatga
    11881 tccccatcta actccaaatc acaaggaatg agttcaaaaa ctagcttttc tcaacaaata
    11941 ttacataaaa aattctatgt tttttgttaa atttatttgc ttttttcaaa agtatgttac
    12001 agtaatttcc caaggaggtt ttacagaaat gaattttgtt gccatggatt ttgaaactgc
    12061 caatcatcag ccttatagtg cttgttctct tgcgctagtc atggtaaaaa atagccagat
    12121 cgttgatgaa ttctatacat tgatacagcc tgagactcct tttttctggc gaaatgtcca
    12181 aattcacgga atccatcaag aagacgtacg caatgcgccg aaattcccag aagtctggca
    12241 aactatccaa ccatattttc aaaaaaatcg tttagtcgtt gcacataatg ctgcttttga
    12301 tacaaaggtc ttagcgggat gcttagatta ttatcatctt gaacagccta attatctttc
    12361 tttatgtaca gtgaaaagta gccgtcgcct gtttcctgaa tttccaaatc atcgattgaa
    12421 cactgtgtgc gaaaatttga acatccaatt agatcatcac catgatgcat tggaagatag
    12481 tcgtgcatgt gcggaaatcc tgctttatca agaaaaaaaa ttcgggacag atccattgaa
    12541 aaaacttgtg actataaagt aaaagaatac ttatgaaaca cacaaagagg tcgtgagcct
    12601 gccgtttcac tccgagtcac aaggagcaat ttcaaaaaat agcttctcat gtttattaaa
    12661 tattgtgact ggtgaccgag gagctggcag gagaacaccg tttattggag aggttgtgaa
    12721 aaagctgttc tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc
    12781 atttggttgg acttattacc gcagatgcaa gcttttggac accgtttacc aagaggttgt
    12841 gaaatcagcg ttttgacctg agtattagag aaacaaacgg caaaatagtt cctcatattt
    12901 tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttat tcaaaaaaaa
    12961 tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct tctcaaaagg
    13021 atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc attttatata
    13081 catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct tcgatttgga
    13141 agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg tcaagctcca
    13201 atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc ataagagtgc
    13261 ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc acgtgagaca
    13321 aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct tcatagattg
    13381 caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct tctaaggttt
    13441 ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc ttattaactt
    13501 gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc aagatgatcc
    13561 caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt gaaggtttca
    13621 ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt atcacttttt
    13681 tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt tccccattca
    13741 acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc tccgctagct
    13801 gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata ttctctaata
    13861 atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc aattcctttc
    13921 gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc acctggcatt
    13981 gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga tagttcgatc
    14041 atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa tgaagttttt
    14101 ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag ttcgcgtgta
    14161 tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat acatcttctt
    14221 catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga attggtccga
    14281 ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct gactgcaatt
    14341 ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct ttcagttgtg
    14401 caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg tattgtggcg
    14461 ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc gatttcgctg
    14521 agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac aattctgctg
    14581 tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc acgttgatcg
    14641 cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc atcgctccgc
    14701 ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga acagtgtcgc
    14761 cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac gtaagcatcg
    14821 taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat gcctttccat
    14881 ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta tcagggctga
    14941 tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt atttcttctt
    15001 cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca gctgcgccta
    15061 aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga acaattttaa
    15121 atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata tgtattatca
    15181 tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa aatgctggaa
    15241 aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc tgttttatta
    15301 cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga gcgcatcatt
    15361 cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg gactgggatg
    15421 tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt cttgtgccgg
    15481 ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt caatgatcgt
    15541 gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt cccgaacggt
    15601 aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat ttccatgttg
    15661 aacaggtgcg attccaagat catcataaag ctctttatag atatttctta acgatggcgg
    15721 gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt ctccatgata
    15781 aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt ccaatgcttc
    15841 atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt ttaaaaattc
    15901 tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt gccaactgtt
    15961 atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca cattttcttc
    16021 aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact aggaacaaga
    16081 agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg gacggcacat
    16141 tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt tttgcaaatg
    16201 atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct cgcgcagctt
    16261 tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag gtatttgata
    16321 aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc tctgttttag
    16381 atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt ctttattctg
    16441 aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat ttacctcacc
    16501 ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg cacatcacat
    16561 acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta aaaattattt
    16621 gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt tctcttttag
    16681 gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat aaaaacgctc
    16741 aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta gaagaagtca
    16801 tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga gtcagtttcg
    16861 caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg gacacaaacc
    16921 ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg tagttttatc
    16981 tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct tggacgtgca
    17041 actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt tggtcgctgg
    17101 ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt tgctcacatc
    17161 aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt cgaaaaggtg
    17221 ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg tagacttcac
    17281 tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt caatcagcaa
    17341 atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt ggatgtctca
    17401 aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta ctggctctac
    17461 cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag ttaaaaagat
    17521 gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc ttttgcatat
    17581 gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca tggcgttcgt
    17641 ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg gagcgattag
    17701 ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct tgtcctctaa
    17761 aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt tctttatcaa
    17821 tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc agtggctgat
    17881 cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact ttaccgtcta
    17941 tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg attgcttctt
    18001 ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt tccatatctt
    18061 ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc gcaaaacgaa
    18121 tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg caaaatcgtt
    18181 attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg attcttgccg
    18241 cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt tcgactacat
    18301 tattgacttc ttcgtatgca aatttttttc cgcaattagg gcatttcatt ttttccatgt
    18361 tatctcaact tcctttcatc tttactctct tagcttactc ttatattgta aaaaagagaa
    18421 gcaatatcct tcttctatgc agatatccat acctgacggt cctgttctcc atctattaat
    18481 cggactttat aataaggacg ttcatctttg actagaagtg tccactcttt cacttttgct
    18541 tttttcccgt agtagctgac tgcttccttc atcgctgctt gcggttgttt gaactcttcc
    18601 gttttttcta tatcaaaaag atgttctgcg gccttttccg tgtcatttcg tacggttgtt
    18661 ttcccagtgg atggattgac tgctatgact tgtccttcac aaaaaaactg gtattcatat
    18721 gttgcattcg taggattctc caaatctttt tcaactgcaa aatgaatagc agatatgtta
    18781 tgttcttttg tttcgttttt tacgattgct gctgcttttt tcaatgagat ccgatattcc
    18841 tggtcactaa tatcatccat gtgaaaagcg gcattgagca gtcccgtcat accggtaatc
    18901 cctattacga tgagggcagc aacaccaaac atcatttttt tcattcttca ttctcctgtt
    18961 tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa acgaaaaaaa
    19021 agtgacaatt ttgttatctt tattcgtgca gttctaatgg caatccgtct ggatcaaaga
    19081 aaaacgtcat ttttttcctg taaaggtatc ttctctaatc ggttctgatt cgatccctaa
    19141 ggactgcaat tctgcaattg tttcttcaat atcgtctact ttgaaagcaa gatgacgcaa
    19201 gccacaagct tctggatagt ttggtcgttg tggcgcatta gcaattccaa aaatctccaa
    19261 ttcagcactt cctagtttca gatctaattt atagtcatct cgttctttgc ggtaattttc
    19321 tctgattact tcaaatccta atttattaac ataaaaatca cgtgatttct gataatctga
    19381 aacaatgatg gctatatgat ggatcgttgt aaggttcata tgagcactcc tattcttcgt
    19441 ttaagctcgg atctatatta atctccagtt ccattgtaaa tgtgtcagat tgaaaaaaat
    19501 agctgttttt cctttttata agaaaaaagg caaaaaaaac agaaagtagg tcctccaaga
    19561 cactcttcct gttcccaata taataaatga aagtaagaaa ttatattttc aagaaaacaa
    19621 acagaaaatt atcataacac aaattattta taattgaaag actagttttt tttcaagaaa
    19681 agcatacaat agattgtgtt agtaaataca gaacacacta catttagtgt cttaaggagt
    19741 cctcatacat gaagtcatat tttcagtttt tagctcatca gctaaaaggc tggccgcaac
    19801 aaaattatta tcttttcttt ttcagtcttg gctgccaggt catgactctt gtcaatagtc
    19861 ccatcacttg gctgtccgtt attacattta tcggtaccac gcttggtgta ttgtgtattt
    19921 tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg tattttgtct gcgatttgtt
    19981 ttattatcgt tgggttttca gcaaagaact atttaagtat cggtgaacag attgcttata
    20041 tgatcacatt ggatatcccc gtattgttaa gtaaagaatg gaatcataat atggcttcaa
    20101 aaatccgaaa attcactgga aaaagctggt ttatcgcaat catcagtact ttccttgtct
    20161 atcttgtttc aggattcctg attggtcgtt atacagatga tcctcggccg tgggtagatg
    20221 ccattgcttt ttctattagc ttgactgctg gtatcatcag tttcttgcgt tacaacaacc
    20281 aatattactg gtggttagct tcagggcttg ctcaaatggt cctttggtat atttcattta
    20341 cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc tgtttacttg ctgaatgatg
    20401 tccttgcatt taccgtttca ccttggtata accaaaaaga acgtgaacgt atgcaaaaaa
    20461 tggaagaaga atactacaac gcacaacaac acgcataaaa aaactgagcg tgggacaaaa
    20521 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaatc agaagcaact
    20581 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct
    20641 tatttcttgg agttaaacgc ttctgtccca gcctctattt tttatagtaa ttccttcaga
    20701 tactttcttg cctcatcatt gaaacgcagt gattcttcta acttctgata atattccaac
    20761 gcttgtttct gagcttcagt aaaacgataa tgttttcctt tccatttgtc gattgtctct
    20821 tgcacatttc cagattggat atctttcact actagttgcc atttttcttt tgagactgta
    20881 cggtctttct tatcagcaga ttgtatagct tctcgttctt tacgcaatac ttttaaaaaa
    20941 ccatttctga ctatccgagg aatgctgtcg aagaaccctt ctgtaaaacc atagcgacaa
    21001 tattgcttcc aaataaatag atagattttt ggttggatga atcgttggtt tttcgcaaaa
    21061 tattccatct gagcagtgac ttctggtgtt cgttgacgtt ttgtctttct caacacggaa
    21121 ataaatgttt caaaataagc attaggatgt actggcactt cttcatatac gatggattgc
    21181 caatctttac gtaaaaatgc gcgatagttg atcaagaatt cttctacatc ctttactaat
    21241 tcttctgcaa agtcgtcaaa tagttctttt tttatctgtc gcttttttga ctgcccattg
    21301 atgtgttcac ttattttctt gatttcattt tccaatgctt gataatccgc tatgtaaatc
    21361 ggcatttcta cttggcgaaa ttctgcgagc cgctgagcta attttatatc cggtaaatat
    21421 ggctgtttca tccgacgatt ttggtacaag acaaaacagt atttttgcca tagtccttca
    21481 ggaaaatcga tattcttctg cttaagccat aataattcgt ctaaggccag atcaactttt
    21541 gtcattccct gatggatcga agctgccact gtcggagaga catgcaggtg atcggcaaag
    21601 tggttgatgc ctagttttag gatctttccg gtttttggcg atttaacgat gtattgcact
    21661 tttaattctc taccacactc acaatacaat ttttgctggc ttcgttttgt tcgaaaaaac
    21721 ggattgatct tgaagtcaga aaacacccaa tctgttgcag ctaaataatg ttcttccata
    21781 aaacgagagc tgattagata tcggcggtgc ttatcgacta gttcccgctg ttttttcgtt
    21841 aattttgcat agatctccca acgttgactg cttgtgtatt gtttagaaat tttgatggga
    21901 tagcttttgc tgatttcttt tggaaaccat tcttcaaaga cggattgcca ttcttccacg
    21961 gctgtttctt tcctttctaa ttacaaaaag ctggctacta acataacgtt agcaaccagc
    22021 cagtgtttct tgctaaatta gtgagttgtt ccggcatttc cggaaatatc ttctttattt
    22081 tctactgctg cttcaactgc ataagtcaaa gaagaagcaa tgtcttctaa agacatagaa
    22141 ggcgtaccca ctggacgatc gatcacttga tcgggtgaga atggcacgtg gatgaaccca
    22201 cctcgaattg ccgggaattg tgtttcgatc aaatgaagca aacgatacat gatatcattg
    22261 cagacaaatg ttcctgctgt ataagagatg tacgctggta gaccatgatc ttgtacattt
    22321 tttgtcattg ctttgattgg caatgaggta aagtaagcgg ctggaccgtt ttcttctaaa
    22381 gccgtatcga atggctgatt tccttcatta tcagggattc ttgcttctgc tagattgatt
    22441 gcgacacgtt cgaacgagac tgctgaccgc ccacctgctt gtccgacaca gatcacgaca
    22501 tctggttggt gctgttggat ggcttctttg actacttgac tgcttttttc gaatacggtc
    22561 ggtacttcca ctttgatgat ttcagcaccg ctgatttcat ctggcatttt ttttactgct
    22621 tcataggctg gattcacttt gtccccacca aatggatcaa aacctgttac tagtactttc
    22681 atctgttgtt cctccgtccg attgatttaa catatggtta tcttttatca taccattttc
    22741 cttgaacagc gtaaataaag gaaacaatag tttaaataac ataacagatc aaacttaata
    22801 tattcttata aaaaaatagt gttccactac aatgttagaa gaggttgcga caattattct
    22861 gtcacaaccc ctctcttcta ttatttaaat agaatcatat aagcaatttg gaaaactagc
    22921 ataaataacg caataaacaa ctggtttttg atcactccgt atttgtcttt catctccagc
    22981 atcgctacag gcacgatatt gaagttagca gccatcggtg tcagcagggt cccacaaaaa
    23041 ccgcaagtca atgcaaccat tcctattaac gctggattcg ctccatgact aaatacgaat
    23101 ggtgcaccga tcccaaccgt catcacggtg atggccgcaa atgcattccc catgattacc
    23161 gtaaatatca ccatgccaag agcgtaaatg atgatgccga tgttcacatt tccttctgga
    23221 acgatcgtac ctactgcatt ggaaatcact gtcccaactc cagcgcttgt aaataccgca
    23281 cctagagaag ctaaaagcat tggcaacatg cttaacggcc ctactgtgcc cagcatatct
    23341 gctgcatcat ctaaaaatgt acttggtcga ttatctcgag aaaagaacat caagattagc
    23401 atggctgcaa aaacaccgac gcccacgccg actaaagcac ctaaattggt aaatagagca
    23461 aacaagatcg caaagacacc gatactcaaa gctggaataa aaattttcat tcctagtttg
    23521 tctgacattt tttccatata ttcttttgat ggaagccggc tttctccttt gctgactttc
    23581 ttagcaattg ctggtatggt catcgcaaag atcaagccac cgttcacgat tgccggaagc
    23641 catcttcctc ctgcaataac tacccctaag accgtccaga aaaaggctga cccataacga
    23701 tgaggattgg ttttatctgt taaatttctt actccagtgt aaatcaatac gagtcccatc
    23761 acgatataaa ggatctccag taatttatcg cctaataaaa tatcaggatt tgtaaaaaag
    23821 tccatcttat tgcccctttc ctggataata tttcttcgct aatttctttt ctttaagata
    23881 gaagtagaca cttgccacaa ctaaagccgt gatagccact ggaacttcca ctaaagcaag
    23941 ctgtcccaat gttacgtcat atcctagatc tttcagtgtt gattggacta gcaacgcccc
    24001 tgctcctccg atgaatagta cttggccaaa gaaccagcag acattttcca tagctgaagc
    24061 catacctttt agttcttctt catgcttagg atggacttct aacccttttg attcgatggt
    24121 ccccaatgcc atcggtaaaa tgattggacg aacaaatcca gcaactccac caaaactcac
    24181 gttaaaagcc gcaaagatca ttcgaaagat cccataaata tctacaatca taccagcaga
    24241 tactttcttg aatcgcttga tcaaggaagc cgctgattct ttcagaccat ttctttcaag
    24301 cgttcctgtt gccaacataa tgatgataaa aatcgccatc ccgcgattgt ttacaaaact
    24361 tgtacctaaa agttctaaca ttccttgaat gccaagaccg ccagtcaaag ctgtaacaac
    24421 taaagcaacc attacgatca atatcgagtc taatttaaat gcaaacccga gaataacaat
    24481 caagacgcct aaaaggctaa gataattttc catatatttt cctccataaa atgacaatat
    24541 ttttttaatt ataaagcaaa acacataaaa aatatagttg ttttctcacg aaatagacac
    24601 tatttttaaa tatttttcgt tattttttta tcacaaaaat tatcttactt attgtaaggt
    24661 aaagcttttt ttaatttatt gcccgataat atatagaaat aaggtctagt tactgttttt
    24721 tttaaatgat taataaataa caacttcttt ctatgaaatt gcaaagcatt catcttcttt
    24781 tccgctaacg atatatccgt cagtgcttgg tacactttac taaaaagtat gagattgccg
    24841 atatctggat aggtgtgcta tactacagaa aaagatatac aatctgccac cgggtaggat
    24901 aaagaggatg atcattccat taggtctttg aagggatgat tttcctcttt ttgtgtattt
    24961 ttttatagtt aaggaggaat gtaagtatgg catggatgga attaatcata gcaggaattt
    25021 tggaagtatt ctggtcaacg atgatgaaat ggagtgatgg tttttctaaa atcaattatt
    25081 ctttttacac agtgattggc atgattgcta gtttttactt tttgtcaaag gcaatcaaaa
    25141 gtttgccgat gagtctctct tatcctatct ggacagggat tggcgcagtt ggttcggtca
    25201 taattggtgt ggtcttgttt catgataagc tgaatctttc cacttggttc tttgtgggga
    25261 tgctactcat cagtatcatt ggcatcaaga ttactagcgg ccactagaca cttggaataa
    25321 gtactgatca gaaaaaataa gcctttttat ttaatattca atttttttat gaaaaaataa
    25381 gaaataagtc caatgaccta gcgacttctc cactcatcag gtgtatgctt gtactatcta
    25441 tagaaaagga gtgaatattt ttgaaaagtc tttcggtcca agaattatcc aagaactttg
    25501 gtcacaaaca agccctcagc gatgtcagtt ttagcttatc ccctggcaaa atcgttggat
    25561 tagtaggtcc taatggcgct ggaaaaacaa cgatcatgaa agctatttta ggactgatcc
    25621 attactcaga tggaacaatc tgtattggtg aaaatgagat ttctccctct agtcataaag
    25681 ggttggaaca agtaggagca ttgatcgaat acccgggtat ctatccattc ttaacaggct
    25741 atgatcatct gaagttattc tctgaaacca acgatgtatc tgctatcgac actatcgtga
    25801 atcagctcaa gatggaaaag tacattcaca aaaaagcgaa atcctactcg ttgggaatga
    25861 aacaaaaatt gggaattgct cttgccttac tgaaccatcc gtctatcgtt attttagatg
    25921 agccgatgaa cggtttggat ccacaagcaa caagagatgt gcgaaaaatc attaccgact
    25981 tggctactca gggcacctct ttcttgatct ccagtcactt actcagcgag cttgaaaaaa
    26041 tggtcgatga gctgatcctg atcaatcaag gaaagatcat caaacaatgt acaatggctg
    26101 aattgaacca aacagcgcaa gatttcatcg ttatctctac aacaaatgat ctagctgctc
    26161 gaacgatctt gacagaagct ggatatactt tggaagatcc tgataaaatc aagcttttaa
    26221 aagaaaacga agaattatta ggaaccgtga tccagctatt gtctgcacaa aatatccatg
    26281 tgacagacgt ccagcatctg acgaatgatt tggaatcttc tctattggaa attctggatt
    26341 cagtaaaggg ggaagaagaa aaatgacatt gcttgtaaaa caagaattat ttaaattgat
    26401 caagaaaaag tcaactgctg ttttatctgt tctattagtt gttttgctca taggaacagc
    26461 acttctagcc aaaaaataca cgacgatcat tgatccagtt gaaatgactg ctcagctttt
    26521 tagtgccaca tcttggattg tctttatcat gattgcagct gcaagcacga tcatttcgat
    26581 ggaagcacaa tatggtacat tgaaaaatct cctttatcga aagtattctc gaggagaaat
    26641 attagtaagc aaatggatca cattagttat ttattctgtt tatttatacc ttttagctat
    26701 tattgtcact gtgttgatga aactgattct tttcccttcg atttccttca cggagaaggt
    26761 ttctactggt caaacactga tccaaagctt gttcacttat actttgggta gttatatcgg
    26821 tttatggctg attttaagtt tagtgttgat gattgcttgc tttatcaata gttccggcgc
    26881 atccatctca gcaggaatcg tcttttattt tgcttcgtca atcatatctg gtatccttat
    26941 tgcactcatc caaaaatggg agtggatcaa atggaatccg atcagtatgt tgaatctgca
    27001 aaaccaaatt ggaaacgaag agatcatgaa gcaattgact catctttcta ctaatcaaat
    27061 gctattcggt aatttagctt acatcgtttt gttcttagca ttaggatatc tcgtatttaa
    27121 gcgaaaaaat gtatgatgac tcatcaaaaa agaggctgtg aaagagacgt tcagccttat
    27181 tgccgattgg ctgtctctta aacaccgttt attctgaaaa aggagctgtg acaacatttg
    27241 tgtcacagct cctttcctag tatgtttttg aataaacgac aggctcacaa cctctcttta
    27301 tacttctttt atacttctat acgtgaagca tcccagtggc aaataaaacc attgcaatca
    27361 ctccgaccat cgtcacacct ataagtaaaa tcttatctaa gtggatatgt tgtacaggct
    27421 cgttttgctc cttacgtgct ttgaggtaaa aataaatgcc tggcaagaaa agaacaagaa
    27481 caagcattac ttcattaaag gctacgacta gcatagccca tacttgaaag gcacttgcta
    27541 aaagccctac taaaaattgg acccattctt tccgctgata agaaagtttc atttggtaca
    27601 atcctaagaa aagccacgtg aagaaaatag ctgctgtacc aagtgtgtaa gcaaagttat
    27661 aagcactgtc agtgaataat aatgaaagta aaaagacatt cgtacataac gtcgtcaata
    27721 cgatagcata agttggtgcc ccatttttgt tgagttttcc ccatgcttta ggcaaaagtc
    27781 cgtcttcagc catgtttccc attgtttcaa tcggtaagat cgtccatgag agccaaacac
    27841 caactaaaga aatgatcaaa ccgccgttga tcacgactgc tccccattta ccaaccatca
    27901 tttcaaacac atagcccata gcaggctgcg ccttgatcgt ttgcagctga ttcatcgtca
    27961 ttgtcccata tggaagcatg gaaaccaaga cgtaaataat aatcaagccg aatatcccta
    28021 gaatcgttgc tttcccaaca tctgattgtt tttttgctcg tgaacctacg acagaggctc
    28081 cttcaatacc aacaaatacc caaagcatca ccatgatgca actcaatact tgactggaaa
    28141 acgggatcgg ctgtccattc gcattcaata taatgtcgct agaaaagtta tcaatgaatt
    28201 gttggatcga aaacgtgctg atagctacta caataaataa aaataatggc actaatttac
    28261 agaatgtgac gatagcattg acgaaactag cattttcgac tccccgattc actagccatg
    28321 catatcccca taaaaagact gtactcatca cgatcgatgc cagattttgc ccaccttcaa
    28381 atactgggaa gaaatatcct aatgcgctca tcaacaatgt tgcaaaagct acgttgccta
    28441 accagcatgt gagccagtaa ccccaaccgc tgataaaacc agcgaaacgg ccaaaacctt
    28501 cttctgcgta actaaagatc ccgcctttta aatctggacg ttttttattg atattattga
    28561 tcgacaagga gagcatcaac attgctgttc ctacgagtac ccaactgaaa atagctgggc
    28621 cgccagccga tccggagatg tccgtcataa taccaaatac gccacctcca atcgctgaac
    28681 caattactaa ggcagcaagt ggaattaaac caattccttt tttctgttgc tctgtatcca
    28741 tgtaagcatc ctccaaaata aaaccgcttt tatcaaaaag tataacaaac cttttttatt
    28801 tttacttaat taataaaaac tttaatagaa atataattct accggctttt ttctcattat
    28861 acaagcaaag cgcctagtcc ctttccaaat tacaccacca aaaaacttaa aaaagatgct
    28921 atactaaaag gaaagaattg gaggatgatc catgaacatt ttatatatat cgatttcagg
    28981 aaatacgcgt gcctttgcca aacatctagc agaatatgct gaaaaaatgc atgcagaaga
    29041 tcctgtcaat cctgaagtta ctttaaaaga aatccatgaa aatagtgatt ttacgaaaga
    29101 aacagaacca ttttttactt tcgttcctac ttatttagac ggcggaaatg gtttagataa
    29161 cggtgataca gaaatcttga ccgaaacgat gcgtgaatat ctggaacatg aagacaatca
    29221 taaactttgt cttggtgtag tcggtagcgg aaataaaaac ttcaataatc agtattgtct
    29281 aacagccaaa caatatgcac aaacatttgg ctttcctttc ttggcagatt atgaattacg
    29341 gggaacacca tctgatgtga cacgaattta tcagatttta gcagaaaaca ataaataata
    29401 aaaaagaaga agctgaaaaa tagatttcag tttcttcttt cgtttctatt gtttctatca
    29461 tattaagctt tgacatccat cacaggtgct tttgcttttg ctttacctgt tttttccaag
    29521 acaaagtttt ttacacgatc catattggtt accaagacga tcatggatgt ttctttgcct
    29581 gctgctttga tcgcttctaa gtcagcttct gctacaagcg tatcaggtgt cacttgctgc
    29641 ccttctgtta ctttcacatc aaatggcgta ccgcctaagt caactgtgtt gattcccata
    29701 tgaagcaaga tttctaaacc atttggcgtt gtgatcccaa tagcgtgttt cgttgggaag
    29761 acagagctga ctaccccagt aactggagaa tagatatgtc cgttttctgg ttctacaaag
    29821 tagccttcac ccatcatctt ttgtgcaaaa actggatcac ttgctttatc taaagaaaca
    29881 actgttccgt cagcaactga tgagaggata gtcacacttt cagctgaagt ttgaggtact
    29941 actttttcag gcacttcatt tactgtggct gtttcagtcg ctaattcttc tttttctaaa
    30001 cggtctttag gaataccgaa gaagaatgta gcaacgaagc caccagcata agctgccaat
    30061 aatccaagga tgtagcctaa ccatttaccg tcagagatca atgggatcaa agcggctcct
    30121 gaaggtccaa tagcgatagc cccgacattt ccgatcattc cgatcactgc cccgccgata
    30181 cctccaccga tacaagcagt aataaatggg cgtcctagag gtaatgttac tccgtaaatc
    30241 aatggttcgc cgattcctag gatcccgact ggtaaagctc ctttgatcat ttctgttaat
    30301 tctttatctt ttttgcagcg aatccataat gccaatgctg cacccacttg tcctgcacca
    30361 gccatcgcaa gaattggaag caatagcgta cgaccagttt catcaatcat ttgcacatgg
    30421 attggagtca aaatttgatg cagtccgaac ataaccatcg gcaagaataa tgtgcctaga
    30481 acgaagccgg caaatgctcc accaacgcca agtacccagt tgatcccacc gaccatgcca
    30541 tcagaaatga aacctgctaa aggcatgatg aagaaaattt caatcagacc gatggcaatc
    30601 aatgaaagag ttggtgtaac gatgatatct accgcatcag gtactacttt atgcagtttt
    30661 ttctcaacga ttgacaatag ccaaacagcg aagatgacac ctaggatccc accttgtcca
    30721 gctgcaagag cttcaccagt aaataagttt ttcagtggtg cttccggatt catccctgtc
    30781 agcatcacaa cggccccgat aactccacca agagttggtg ttgcaccaaa aacttgtgca
    30841 gcattgatac ctgtatagat aactaggtag ctaaacatcc cgtttttcaa gatgttcatc
    30901 acatcgatat attgttgcca tgttgctgca tcaagtgttc cggctgtgac taagtttgct
    30961 aaaactgcgg caatccctgc cacgatccca gtaccgacaa atgcaggaat cattggcaca
    31021 aagatattag aaatgtcttt taataccgat ttgattttag atggtttttg tttcgatttt
    31081 tgtgccgctt tcatttcttg tgcccgttcg tttacacggt cttttccaga tgctgatgaa
    31141 tttgctgtag ctgtacttac agaagaaagt ttttctccta atttcacgcc tgccaaatca
    31201 accatttgct gtgctacttt gttcactttc ccaggtccga tgatgacttg aagctgttca
    31261 tcttctacta caccaagcac tcctggaatc gcttttaatt cagagacatt tacaagatca
    31321 tcattgatga tcttcatacg cacacgagtc atgcagtgga caatgctttc tacattgccc
    31381 atgccgcctg cccctttata aatttgtttt gccagttctt ccaaagtcag ttcgtttttt
    31441 gccatggtct tttccccctc ataaggtccc ttttacaaat ccgtttgctt cagctaattt
    31501 tgcttgagct tcctctttat cactatttgt caaaatcatg acgatagcca gcttaacatt
    31561 ttgatctgct tcttcaaagt acttttcagc tgtttcgtaa tcagcttctg tcgcttgcat
    31621 gatgattcgt tttgagcgtt cgattagttt ttcatttgtt ggtttgacat cgaccatcaa
    31681 gttattgaat actttaccta taccaatcat actgatagta gaaatcatat tcaagataag
    31741 tttttgtgct gttcctgact tcaatcgagt agagcctgtc aaaaattctg gacctgcatc
    31801 gacttcgatt gggaaatccg cgtgtttact gatttcggct tgtttattgc aagaaatcgt
    31861 tcctgtgaca gcaccgatcg attttgcgta atctaatcca ccgatcacat atggtgtacg
    31921 accgctagct gcgataccaa taactatatc attttttgtc aaattcagct tgattagatc
    31981 ttcttgccct agctcataag aatcttcggc cccttcgaca gctacagtca ttgcttcttc
    32041 tccaccagca attagaccta tgaccatttc cggttctaca ccgaatgtcg gtacgcattc
    32101 agcagcatct aacacaccta gtcttccact tgttccggct cccatataaa tcagacgtcc
    32161 acctttttta aaggactcaa tcgtgttagc aatcactggt tcgatacttg tcagttcttt
    32221 ttcaaccgca gcggccactt tctgatcttc tttgttcatt agttgaacgg cttgagaaat
    32281 actcatctca tctaatccaa aagtttcttg gtttctgcgt tccgttgtta ggttctctaa
    32341 attgattttg ctcattttta tcttccttct tttcttataa attgatagtt cataccagct
    32401 ttgatttggt tgatcaatgg tagctcttct ttgataactt tagctactac attcactttt
    32461 tcatccgctg gcaaatcata tttggttaat tggatctcac ccatatagcg caaatacttt
    32521 tcgttatcta aagtaataga ccctttcata cgcgtagctg tatttcttgc tgggataggc
    32581 ggaatctctc gaaaacgtgc atctacgctt cgtatgacat ctcttgcatc gtcttgccga
    32641 ttcgtatgct tccctaatat atattcgtaa aattcttcat ctacaggttc cgtatggagc
    32701 aagactgtct gttctttttg gaaagaagca aattgctcct gaacgttttc tgacagacct
    32761 gcatctccga tgtaaacaat atccgtgtta cttgctaata gatctagagc gcctgcaaga
    32821 ggatggatcc ctcgatgttc ttccaaagtt ggcaatcctt gatatagtgg gcctctcaac
    32881 tctgtatctc ctggtacaaa accttggaca aggagacctt gaattttgag ccattgattt
    32941 tttttctgat accattcttt gtccagccct gtttcgggac gtggataata attatgccaa
    33001 gcttcaatct gtgagaaatc agcttctgct tctttcaatt catcgatatc tttaggcgtg
    33061 atcgtgctag cattcaaact gattttcaat tgatgtgacc agtcggcaat ctgctgattg
    33121 gaaatatgat aatccattct taaacctgtt acaccaattg ctttcaaagg cgttagatct
    33181 tctaatgaaa aacccgctcg ttccaatgcc tctcctgaaa tatcgaccat caattctagt
    33241 ttattctttt tcgcttcgct tcctagttcg atcaatcgct tttgatatgc cgaaacatca
    33301 tcttctggta tatgcatgga agtgaaaata ccgacaaagc cattggatgc catttggcgg
    33361 atatactgtt tcttctcttc tgacaaagaa tcgttcataa atattgaaaa gccaaacata
    33421 ttatccctct ttttctgtat ccgtcttcat ttctatgact agagtatata tctaaagaaa
    33481 caaaatttca atacttttat taaaataatt aaatttcatt tctaaaggtt gagtttatat
    33541 tatcaaaaga attgtcataa cctaaaaaag gcactgctaa gcaaaagcag tgccttgaaa
    33601 tattttattt aaataacaca aaaacataat tactcataag aaaccaattc tgtattatct
    33661 accatgaatg taaagaaccc ctcattaggc atcggatgaa aataagcatt gatcactctt
    33721 atcaaggcat tatggcaaac taacagatag acattttctt cgtcttcgat gcattctttc
    33781 aataaaggaa cgatccgtgc atacacatct aagactgact ccccatttgg gaatcgtaca
    33841 gcgaacgcta aacgtgcttt ttgaaagttc tcatctttgc ttggcagtcc atcataatcc
    33901 ccaaaatcca tttctaccaa tcgttcatca acggtcagcg gcaagctgag acgatcagca
    33961 actgcttgtg ctgtatctct tgctcgttga agaggggaat ggatgatttt cgtgattgga
    34021 acaggcaaat ctactacctt ttctgctagt ttctgtgcct gcatctgtcc tttttcagtc
    34081 agcggcacat ctgcccgtcc gcaaactaat ccttgtgcgt tccaagttgt ttctccgtgt
    34141 ctcgttacat aaagcatgtg attctctcct tcgttggcaa agttcccctt aaccatacct
    34201 tgttgttctt gagattacaa gggttagatt ctctcaagag aaataatctc agtagatact
    34261 ttatccagga aggtttgatc atgttccact agtaacatgg caggtttcac gttcaagatc
    34321 agttgttcta attgttcttg attgaagaca tccaaataat tcaatggttc atcccatgta
    34381 tatagttcag ctggctgtga taaagattta gccaattcca cttttttccg ttggcccata
    34441 ctcatctgct cgatcttgtt atgaaaaaca tctctttcca tcccaagctt tcggaggttg
    34501 ttcaaaaatg cttggtagtc tacttggttt ttctctgcaa attccgccaa cgttcctcga
    34561 ttgtcttcat aattttggct tgcataacta atgctcagat gttttggctg gtatttttct
    34621 cctataacct tgccgttgaa tgcccccaga agataatgga taatggatga ctttcctgca
    34681 ccgttaggac ctgaaatcgc cacccgctga tgaggctcga ttgtaaaatg aattggctcg
    34741 aataacagat tttcataccc taattgaaga tcttctacgc ttaaaagtcg cttatggtga
    34801 gacgcttggc tattcatcgt caaagaatcg atatattcga tatcttttaa tagtttttct
    34861 ttctcgctga tctgggtctc catccgattc acgattgctt tggatcgttt catcgtccgt
    34921 gcagcatcag cacccactgc tcctttattc actcgtctag attcagtatc gatgaatccg
    34981 acttgtttct ttgttttatc tccttctcgg gaacgagacc attcggcttt ttcagctgct
    35041 gttttcttta gcctactgac ttcttttttc aatttttcat tttgagccat ttcaaattca
    35101 tcacgaagtt ttttctgttc ttcatagata gagaaattcc cttgataaag ttccagttga
    35161 cttttttcga ttgctaaaac atggtccact acttcatcga taaatccccg gtcatggctg
    35221 accacgatga agccttgttt tttctttttc aaataagccg ctacttgttt tctaccagag
    35281 atatccaaat gattcgttgg ttcatcgatt aacgggaaat gagtgtcatc cacaaataaa
    35341 agtgccagta ggacttttgt cttctctccc cccgatagtg tgctgaattc tctccataag
    35401 atttcaggat ctgtttgcat caattggagc tctctttcga tttcccatat ctcaaaatcc
    35461 gtaatatcat ttaacacgta ataggttaaa cgttctttat cttttgtctg ttgcgggaaa
    35521 taggcaaatt cttgctgatg gattacttgc ccttgataag gtagtttgtt ttgtagaata
    35581 ttcagtaaag ttgtctttcc tcgaccgttt cgtccgataa gtcctagttt ccattgtgtg
    35641 tcaaaattta gatttgcttg ttcaaataat aatgtgcctt ggctgtcgta gccgaatgtc
    35701 agatttttta tttcgatttt agacattttc catcacctcg taaaaaaaga ccagtcctat
    35761 tttcagaact ggctaaagag gatgcaaggg ttggatacac acaaaaagac ccatacatcg
    35821 aaaaaaccaa gccaatcccg aaaatctgca cactgcttcc ttgtctccac aaggtacagt
    35881 ctgaatgcag ctttcatcaa tcgacttaat ttttcaatgc acggatccct cactttgtcc
    35941 tatatttgaa attacgatag cataaagaaa atgattttgt caactacttc gggaggatgg
    36001 tgaaaaatct gctaagtgtc tgtcctaaga tttttgtttt ttctaatgtt tgcctgtgtt
    36061 ccattgcttc ttctaaacct attggaccca aatgaatact taagacaatt gggagaaaat
    36121 cagaaagtcc ttctagctct tggtctctac ttccgcaaat agcgattatt ggttttcgat
    36181 atttttttgc aagcactgct acaccgtatg gaacttttcc gtggatcgtt tgctgatcga
    36241 tccgcccttc tcctgtaatg atgacatctg cttgttcgat ctttttttcc aatccagtga
    36301 tttggctgac taaatcgaaa ccagaaacca tcttcgctcc caacaaaaaa agggctccac
    36361 caataccgcc tgctgctcca cttcctggaa tatcatctaa atccacctcc cagaaatctt
    36421 ttatttgctg agcaatcttt cctgcttgtt tatctaagaa agctagcgtc gtatctattc
    36481 ctcctttttg tcttccaaat tgccaagcag ctccttgttg acctgtatat ggattggtaa
    36541 catctgcagc gataatcaat tttactttag aaacaagaac gtttcttcga tcaaaatgaa
    36601 gttttttagt agataataaa ggatttcctt ctgtgtaccc ttctaaagaa gcacccaagg
    36661 attggagtaa tcctagacca ccatcgctac acccgcttcc tcctaaagtg acaataattt
    36721 gttctacttc ttgaagcagt gcatctttaa taagttctcc taaggcataa ctggaagcaa
    36781 gtcggatagt ttgatccgtt ggtttatcaa gtagatctaa ccccaatgct tgtgcagatt
    36841 cgatgactgc ggtgtttttt ccttgccaag ttgtcaataa atagctgatt tttcgctgac
    36901 ggaataagag atccatccca tcaatggtac gccatgttcc cctgagcgcg taatagattg
    36961 cttctgcagt attttctccg ccatctgcaa tcggcacatt gatttttctt cctttacgat
    37021 cttcccagcc atttagaact gcctgattta gttcaaatga agaagcactt cctttaaatg
    37081 aatcaattgc cgccaaaata tccatctgta tccctcactt tcttcttaag taagacactt
    37141 tttttcattt ttttcaaaca aaacaatgct attttcttcc atttttctat atactaaaag
    37201 ggaactcatt gaataaagga gagaaaaaac atgaaaatcg caatagctgg ggccggagca
    37261 atgggcagcc gaatcggatt gatgctacat caaagcggaa atgaggtcct cttgatcgat
    37321 cgttggcctg ctcacattga agccattcgt acaaatgggt tgattgctga ctttaacggt
    37381 aaagaagtgg tcgcaaaact gccaatctat tcaccagaag aaatcatcga aagcaacgaa
    37441 cacgtggact tgatcgtagc attaacaaaa gccaatcaat tagatgatat gttttgttcg
    37501 atccaatcca ttattactga caatacttat gttttgtgtt tactaaatgg attaggtcat
    37561 gaagatgtac tggaaaaata tgtgccaaag aaaaatatct tatttgggat cacgatgtgg
    37621 acagctggtc tagctggtcc cggaaaagtc acattgttag gtgatggaga aatcgaacta
    37681 gagaacttgg aaccagaagg cgaagcattt acaaaaaaag ttgtagaagt ctttcaagaa
    37741 gcaaatttaa atccgatcta tagccataac gttcgttatt ccatttggcg aaaagcttgt
    37801 gtaaatggaa cattgaatgg tttatgcacg attttggatt gtaatattgc tgaactaggc
    37861 gcgcaaaaag cggcagagag catggttcga acaatcgtat ctgaatttgc ttctatcgca
    37921 gcaaaagaag gaatcatact agatcaagaa gaagtgtacc aacatattgc ttctacttat
    37981 gatcctgaca acatcgggtt gcattaccca tctatgtacc aagacctgat caaaaaccat
    38041 cgtttgactg aaatcgacta catcaatggt gccatttggc gaaaaggaca aaaatatgat
    38101 attgctacac catattgtgc ctttttgacc caattagtcc atgcaaaaga aggaatcctt
    38161 ggcgcagaat aaaaggctct ctgatgagaa tcagctgaat cagcagctgg ttctcttttt
    38221 ttaagcccaa taaaagatca aaaagaaaac acaactctca tgatgccata agaattgtgc
    38281 ttgtttgttt cagtacataa tctcttcaaa atgtaggttt tttatttgtt ttcaacagag
    38341 gagaggtcat attcgtattg ctcataaata gtggaatcgt tagtgataaa tcgttttgcc
    38401 caattttctg catcataaaa ttccaatagc tctgatagga ttggcttaaa tggtgtatgc
    38461 agaaattccg gtaattcatc taaatcacgt tctcgaagca aaaaaatatt ttcaggacga
    38521 taaaaatgat aatgagcaat cgcttgatta tccgtcacta gtttcttttg aaaatacatg
    38581 gattcaaatg tccgcaaagt cactccttgc tgaccctccc gcaacagttc cagaatcccg
    38641 cgcgattccc ctgttttttt caggtactct tcataagtaa gatatccagg ttgttcattc
    38701 cctcgatgtg gaagaatgaa atagttggtc gtaacatctc tttttataaa ttcctgctta
    38761 tagctttctg ctcgttcttt tctcccatta ttcgttgcac caaaaaatag atccgtcttt
    38821 ggttcgctag aagaaaaatt catatgtttc gaataaaatg aactattatg tttcagccca
    38881 agttcttttg cttccaaaaa atcaaaatga taaatctcat cgattgccgg ttcatcttgt
    38941 gcctgcttca gcatcgcctc gtgttctgct ttgaagtgat tccaaaaaaa taacacaatt
    39001 tttgcattgg taaatgggcg cacgcgtcga gccatctcca tatcaaatcc ataatctaaa
    39061 aaaacgatca gctgataatt tgccaactgt tgtctagctg atttaatgaa ttccgactgt
    39121 agtaaatctt ttccggacca aacatctaaa gagcggatgg aaggcaaaaa ataaagtgat
    39181 ttattttttg caactagcag tacgtcattt ttgttcatct tttccgcctc actttctctg
    39241 tcactttttc ctatgatagc ctgtttaaat agtaaaaagc cagtaaaacg tttcctatat
    39301 ttgtatgaaa atttaacatt tgtatgaaaa tatgatgaaa taaatcattc ttctattaaa
    39361 attcgcctaa aagttttctg aatagttgta attgtctgaa aatttgttat cattagtcat
    39421 tgttaaatat tgctatttat tcttaatata aattttgatt gtgaatttaa aagaaagagg
    39481 tcatttatgg aagataaaaa attgaaaaaa caacttagct ccagacatat caccatgtta
    39541 gctttaggtg gagcaatcgg tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc
    39601 gccggccctt ctgtattgat tgcttttttg attggtgggc tgattttata tatcgtgatg
    39661 aaaggtctgg gaaaaatcgt tttaagcggt ggcgacacgc atcacggatt atctggtttg
    39721 atacgccctt atctaggtgc tcattctgct gattttactg attgggtcta ctggtctatg
    39781 tggattatca atatcatcgc tgaagctgtt gcagctgctt cctttttgca gttgtggttc
    39841 ccgcacattc cagcctgggt atttgttttt atgttggctg ttttaactac gatcatcaat
    39901 ctttattcgg tacggttatt cgcagaaaca gaatattggc tggcttttgc taaaatcagt
    39961 gtcattatct tactgatcat ttttgctact tatcttgtcg gtcagcaaat gttgggcaca
    40021 ggcgttttcc ctacccttca gcaattgact gaccatggtg ggtttactcc tcatggtatg
    40081 aagggaatca tcagttcttt gcttgtagtt atctattctt acggcggttc tgaactgatt
    40141 gctatcacgg taagcgaagc agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt
    40201 gtcatgggaa gaatcatttc cttttacatc attcctttgt ttttgctttt gattcttttt
    40261 ccttggaata ctttagcagg cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg
    40321 aatatcccat ttgcagcgga tatcgtcaat tttgtgattg tccttgcttt gttctcatct
    40381 atcaattcgg gtgtgtatgc atcttcaaga attttgttct tccgtttgaa ggatcgaaaa
    40441 ggttcctcta gaaaactagc tgttttaaac aagcatcaag taccgcaacg tgctgtttta
    40501 ttttgtacca gtgttcttta tcttggtgta gctctttctt attttgtcgg tgacaaatta
    40561 tttggctatc tggcaggttc cctttcgtat acagtattat tgatctggat cattatcagt
    40621 gcggcatcct ttgtactggc actaagaaaa ggaacattat gggataaagg aatcagcttt
    40681 tttgcattag ctgtcttagg attgattttc cttggtatcc tgttcacgaa ctccattggg
    40741 gtaactgtat tgactggcct gctttacctg ttcatttatt tcagttatca aaaaaagaat
    40801 gatgcgttcg tattgacgaa tgaatgaaat cgatagaaaa ttttatttta tttatgtgga
    40861 atagatggag ctattgaaga aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc
    40921 gaataaacag tctcatgacc tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa
    40981 ttgattgttc tccatgctca tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt
    41041 ttaaatgaaa aagtattgga agtttttgtt gatgatcggt gtttcaacgg tagttatgtt
    41101 tggtatgatg tacttgaatg tctatgcgct cgatcatatc ttcttcagtc gtacacgagt
    41161 attcatggcg ttgatgatgg gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa
    41221 aatgtatgaa aacaaaaaga taaatgttat gatcttagcc gctagtcttc ttgtttttgc
    41281 cggttccttg tttatggtac gaagccaaac tactgtcaac gacacagctt ggatgaaggc
    41341 catgattcct catcattcta tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc
    41401 tgaagtaaaa aaattagcgg aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat
    41461 gaaaaaaatg atcaaagaaa tggaatgacc attatccgtc caaataaaaa aagaggatgt
    41521 tccagaagtt atcagcttca agaaataaga agaagctact tctggaacgc cgtgaataag
    41581 gggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagcttct
    41641 cctatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
    41701 aagaggttgt aaaatcagcg ttctgacctg agtattagag agacaaatgg aaaaatagtt
    41761 cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa caccgtttat
    41821 cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct
    41881 ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    41941 ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc
    42001 taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca
    42061 acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg
    42121 caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca
    42181 aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg
    42241 atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag
    42301 gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa
    42361 atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa
    42421 ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat
    42481 actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac
    42541 aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa
    42601 aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt
    42661 ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt
    42721 tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg
    42781 tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat
    42841 atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc
    42901 ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag
    42961 cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat
    43021 aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg
    43081 gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc
    43141 tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat
    43201 tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat
    43261 cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt
    43321 taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc
    43381 taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat
    43441 gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt
    43501 ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat
    43561 taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta
    43621 attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta
    43681 accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca
    43741 atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa
    43801 gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg
    43861 ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc
    43921 gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt
    43981 gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt
    44041 tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt
    44101 ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa
    44161 agaaggagta acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt
    44221 gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa
    44281 tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt
    44341 ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa
    44401 gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt
    44461 ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt
    44521 cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc
    44581 gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc
    44641 ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt
    44701 cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc
    44761 aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga
    44821 aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct
    44881 ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc
    44941 aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag
    45001 atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca
    45061 tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt
    45121 ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt
    45181 attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc
    45241 cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc
    45301 gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa
    45361 ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc
    45421 ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc
    45481 atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt
    45541 caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc
    45601 agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc
    45661 aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc
    45721 caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc
    45781 tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg
    45841 gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt
    45901 tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa
    45961 attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt
    46021 ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc
    46081 cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac
    46141 caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg
    46201 cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag
    46261 caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg
    46321 ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg
    46381 aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc
    46441 cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga
    46501 atgctgtcca aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa
    46561 tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg
    46621 atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat
    46681 ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc
    46741 aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt
    46801 acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa
    46861 cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc
    46921 gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct
    46981 tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata
    47041 accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt
    47101 gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc
    47161 ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct
    47221 cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct
    47281 ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc
    47341 gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt
    47401 agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga
    47461 gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc
    47521 aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa
    47581 acatcgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt
    47641 tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa
    47701 aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc
    47761 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca
    47821 agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc
    47881 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
    47941 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac
    48001 ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct
    48061 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt
    48121 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
    48181 gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac
    48241 gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg
    48301 aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac
    48361 agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata
    48421 atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc
    48481 tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg
    48541 ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa
    48601 tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt
    48661 cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat
    48721 ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc
    48781 ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat
    48841 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg
    48901 gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac
    48961 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
    49021 aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc
    49081 gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc
    49141 agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag
    49201 cctaattcag caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta
    49261 gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc
    49321 agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat
    49381 aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca
    49441 gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt
    49501 ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc
    49561 aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga
    49621 ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc
    49681 agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata
    49741 ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct
    49801 aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg
    49861 atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta
    49921 tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg
    49981 aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc
    50041 tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact
    50101 aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac
    50161 gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg
    50221 tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca
    50281 ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt
    50341 cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc
    50401 atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca
    50461 acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc
    50521 ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc
    50581 agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt
    50641 ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta
    50701 tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact
    50761 gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga
    50821 agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa
    50881 tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg
    50941 atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca
    51001 gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct
    51061 tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg
    51121 ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc
    51181 aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc
    51241 atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct
    51301 gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca
    51361 gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata
    51421 gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc
    51481 gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct
    51541 ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa
    51601 ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt
    51661 tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc
    51721 tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg
    51781 tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat
    51841 aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct
    51901 catctccttt actaagtaat tttaattcat gacttccaat catttttcga atagttattt
    51961 ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa
    52021 cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat
    52081 ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct
    52141 tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg
    52201 ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg
    52261 ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt
    52321 ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc
    52381 ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt
    52441 atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca
    52501 aagggactgg ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa
    52561 cattatcagc acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc
    52621 ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg
    52681 gttggagcaa caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg
    52741 ccggggactg aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc
    52801 gcccgctttt tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac
    52861 aaattctaaa ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa
    52921 ggacctccgt tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc
    52981 tttcgtcttt tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa
    53041 tgtttcatag atcactgtac ttctttcttt atgtgataag ctccgtttca tatcttcatt
    53101 cacactacgt gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa
    53161 aaaagtaaag agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt
    53221 gattttcaaa taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag
    53281 aatagatagt tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg
    53341 gcattcataa cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg
    53401 aagattcaaa accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct
    53461 gatctcttta taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt
    53521 ctttaacttt attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat
    53581 gttcattcgc tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt
    53641 ctcaatcccg actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa
    53701 cttcttgttg actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt
    53761 taatttaaat ttttccacat tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa
    53821 aaataaaaaa atgttttttt cataaaaaat accaaccttc aagcattaga atgttgtcta
    53881 actactgaag gttggtttga ttgtttattg attatttttt tgctgttcaa cgtctgttct
    53941 tgttactgga tataagttgg ttgttgtttt attaccattt tttgaaaaga cacttgtcga
    54001 atgttcgcct ttttgctttt cgattgcttc attacgttcc gcttcatttt tatagtcata
    54061 ttttttcgga tcaatcgtct caaatccgtt tggcacataa aaccgcaaga gattttcctg
    54121 attcagctta tctgaaagac ttaacgcagt cttgactttt tcctgatctt tggagatttc
    54181 ctcatcgatt cctgctgctt tcgtatcgat gatttctcct gtctgattat tgtaagcttt
    54241 tcctcctagg acggtgtatt tgggtgtcac aaagttccca tttcgaaaag caactacttg
    54301 atcatgctga gaagataata agtctgtacc aaaatgaaga taatctttat catcgattcc
    54361 caatagatgt agtaatgtcg gcaaaacatc gatttctcca ccgtattcat gattgactgt
    54421 ccctttagta taaccaggca tatggatcat caatggcact cgttgcatct gtgcgttgtc
    54481 aaattcatcc catgtatctg gatctttacc aagagcgctt gctaaatctt tattatctgt
    54541 attcgagatc ccaaaatgat ccccgtaaat cacaaagatt gaattttggt aaataccaga
    54601 ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc agataatgtg ccgttcgaac
    54661 ataattatct actgtgctat tgccagtatt caaagatgga aagtcgaaat tcttgtcatc
    54721 cgtataataa ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg gctgttgtag
    54781 atgctcaaga tattgggcag attctctgaa taaatcttta tctaatatcc cataacctaa
    54841 cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa ttgtcataac ctaagttttt
    54901 atatacatga tctctattcc aaaaactagc tacattccca tggaaaacag cgcttgtata
    54961 gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag acattgtctg acccaagttc
    55021 tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc
    55081 acttgtcttt ccctgtccta cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt
    55141 ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg tctaccttca tattaatcaa
    55201 aaactgctgg aaactttcta aatggagaac gatcacattt ttccctttag cgatcccaaa
    55261 tgtttcggga tttggctctg cataatgttt ctttgtataa tctaaaactt catcaatccc
    55321 attactgctt gcatgcgcgc gaacactgct agtcattcct gttttgattc cgtcataaat
    55381 agtatacgca tcgattccta gatatttgac aatatatgaa cggtcaaatg ttcgctgcag
    55441 caattgtgga cggttggctt cgctcagcgc tagatttcct gaaaaaataa gccctgaaag
    55501 acaggtaaca gcgatagcca tcggtttgcc gacagggttg cttttgatag gtattttctt
    55561 gaccttcaac caaaccagta gcccaatgaa cacagcgata tccagccagt agatgacatc
    55621 atggggcttc atcaaagaaa aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc
    55681 taatacagat ttgactgtaa tgaaatctgt gaattcccga taaaaaataa tattcaagta
    55741 caacaaaagc gtatttaaaa tatcaatgat caaaagaata gctaacgctg gctttggctt
    55801 tttaatatac aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt tgatccacaa
    55861 gataaagtac tggatcgtac cttccacacc taatgaaaaa tctgcatagt aggcaatgat
    55921 cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt gctaaacgat tttggcacaa
    55981 ttttttaagt tgtgtcacgt atatcacttc tctctattca cattttttct tattttgctc
    56041 ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag atttttttat tctttctaaa
    56101 gattattccg tccacttttc acagcataaa accgctgcga tgtaagcgat taaactaatg
    56161 aagagataaa agtcttgtgg tgaccgcttc actccacggt attcaaaagc cagtttctcg
    56221 gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt atttcttgat
    56281 gcaggacagc tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc
    56341 acaagtcaca atatttataa aacatgagaa gctgtttttt gaaattgttc cttgtggctc
    56401 agagttaaac ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccctcgg
    56461 tattagatta tgtcaggacg ctaattttac aacctcttgg taaacggtgt tcaaaagctt
    56521 gcatctgcgg taataagccc aaccaaacga aaaatataag gagctatttt ttgaaattgc
    56581 tccttgtggc tcagagttaa acggcaggct caagacctct tgatacacgg tgttcaatca
    56641 gctgaccttc ggtattagct caaaaaacgg caaaatatga ggaactattt tgccgtttgt
    56701 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tgtttttcat tgtccatagg
    56761 attgatattg tccttgtgat gactcattcg taataattga ggcttgattt tcttcacttc
    56821 ccgttatcgt ttgtccactg ccagaatctg aaggacttgt tcctgtcgac tcgttttttt
    56881 gagaagagac agttgttttc ttgacttgtt catttgtctt tttccattcc gctaaacgat
    56941 cggcgaccac ttttttgtct actttttcag aataagcggt gatcactttt ccatttttca
    57001 aacgggctac tttgtcgcca tcatgagtca aaagcaaaac ggtcaaatgc ttgtctttaa
    57061 attgatattg tctgagagaa atcgcactaa gtacgtcatc gatattaata aattgatttt
    57121 cttcttttcc ttttggcaaa taaatgtcca attcgccttt ttcgtaattg gcagttattt
    57181 tatactccgc ccaccaataa gttgagaatt cttttttgaa attatctact gattctttgc
    57241 ttgctacgat tttttctgtt tctttctgag caagatctgc tgtcattttt cctgaaaatg
    57301 ctgcggataa aataagcagt aagaaaaaac cgaataacca aaagagaaga aacggtcttt
    57361 ttttcctcat gctttcctcc actcctattt aattttgact attctattat atacaaaaaa
    57421 tgaaatagaa agacaaaaaa gttgaaaaag atcgtaaata attgaacttc tttttcacaa
    57481 aatggtaaga atggagaatt tcacgcattt gctctcaact attctttctt aagcctcaaa
    57541 tccgttttta tctgatacgt ctttgaacca atgacccgat tttttgattg ttctttttcc
    57601 atcttgtgct aagtctactg aaataaagcc atatcgattc ttatatgcat tggtccacga
    57661 ccagttatcc atacatgtcc acatatgata gcctaaacaa ttggcacctt cttgaatggc
    57721 ttgatggaca tatttcagat gagattggac aaattcgatc cgataatcat cgtggatcat
    57781 gccatcttct ttgataaatc gtgcttcgtt ttctacaccc ataccgtttt cagaaataaa
    57841 gcagtcgata tttccataat gatctcgtac attgatcaaa atatcataga ttccttgttc
    57901 atagatctcc catccgcgat aaggattcat tttacgacca ggcatttcat aattgtcgaa
    57961 ataatcctca ggcattggac cgtttttagt atcgattggt gtttcttttg cttttacccg
    58021 gcgtggctgg taataattga tacctaaaag atctactgta ttttctttga taaccgtcaa
    58081 atcctctgca tggatcacag gcatgagatc aagttctttt actatctcaa tcaattcact
    58141 tggaaactcg ccttttacag ctggatcaag gaatgaacga ttgaagaacg catctgctat
    58201 ttgcgctgct ttaacatctt cagggtttga ttcgtctctt ggatagcttg gtgtcagatt
    58261 caagatgata ccaataacac caccttgctc catttcatgg taagatttga tggctaacgc
    58321 tgaagccaac gcttcatgat agccaacttg gatcgctttt ttcatatctg actcttctgg
    58381 ataatgccat cgatataaat aacctgcttc gactggaacg attggttcat tatgagtaaa
    58441 ccattttttt actttcgaac caaacaattc aaaacaactt ttggcatatt tttcataggc
    58501 ttcaaccgtt tctcggttca accatccgcc tttttcttgc agatacatcg gcatatcgaa
    58561 atgatacaga ttcacaaagg gttcgatacc gttttcaatc aactcatcaa aataacgatg
    58621 atagaaacga acggcttctt cgttgattgt tccagattca tcaggtatca aacggctcca
    58681 ttggatagat gttcgaaatg aattatgccc agtctgtttc atcaactgta catcctcttg
    58741 atacttcgta taaacttgcg aggttttttc aggtcctact tgatcaaaga acttttgcgg
    58801 ttcttttttg taccaataat caaaaatgtt ctctcctttt ccatcgcctg aaatccttcc
    58861 ttctgtttgt gggccacttg ctgcagaacc ccaccaaaaa tttgctggaa attgatattt
    58921 tttcatcaaa gcacctcttt ttctttttta gttcgtttta gttgattcca tgtatgatat
    58981 tttccatcac aatcactttt tcattttctt cctcttcttt tttgattcgt tttaacagct
    59041 gttctgctgc tcgttccccg attttatctg ttggtggcgt gatcgtgatt ttcggaagga
    59101 tactcagatc gtttttatct aaataatcat agtgactgaa cgcaatatcc ttcccgattc
    59161 tgacaccttc ttgattgaaa cattcgatgg caccaatcga catattgtaa ttgctgacaa
    59221 aaatagcagt tgcccctttt tctaataatg cttttgcccc gtaatagccg ctttttctag
    59281 aatagtctcc tgtaaagacc tctaattgga tcgatggatc attttggacc gcttctttgg
    59341 ctcctgctaa acgttctctt gctacgtagt ctgtttgagt ggcagcaata aatccgattt
    59401 ttttatgtcc ttgttcacca tgatgagcga tcacttttgc tacactttct cgatcattca
    59461 ctaatattgt atcaatacct gctggtcctt ctggacaatt caatgcgata actggaatac
    59521 tcatttttgt gagcatttca aatccagacc actcttcttc ggataagaag acaatcagcc
    59581 catcgatcac atgcttcatc aggtattcaa ttttttgttc aattttttct ggctgatcgg
    59641 caaacccgct taataataag ctatacccat tttcttcagc tactctttcg atccctgaga
    59701 caacttccga cccaaaacgg ctggaaatcc cgtttatcaa aagtccaata gagaagcttc
    59761 gattattctt caacccttta gcaatgatgt tttctttata gtccaacgct gaaatcgctt
    59821 ctgctatttt tttcatgttc ttttccttca gttgctgacc attcaagtaa cgggaaactg
    59881 ttcccactga aacaccagcc atcttagcca catcacggat cgatgccata cattctcact
    59941 cctgccagtt acatttgatt tctctttctt gttgttcgat tatgatgacc gcattctttg
    60001 catagtgttg cttgtcatca atatccatat aactaaagtc ttcagtagag caacttcctc
    60061 tcactattaa ttatacttaa acaagtattt gaaccggttc atttttaata tagcattttt
    60121 ttaaaagatt gcaacacttt tttaaaacgc tttctgaaag tggcggattt tttgcctttt
    60181 taaaatttta ttctaaaatt tactctattt gtgggtgttt tgtgcgtttt tctcttccat
    60241 ccgtttgaag ttttacattc caatggtata atttctttat cctatcaaat gggggaagta
    60301 ttttgaagaa attaatcgta ctatttattt tgtctgcggg acttttgaca ttgactggct
    60361 gtgatttgtt tggcggctct gaccaatcct cggacgaaac aaccgattct tctgtcttaa
    60421 aagcggaaaa agcagatacc accaacagtg tatatacgct aaaagtacgt aaagatatgg
    60481 atttatccgc gggacagttg acatttacag ataaaaaaat ggaatggaca cgcacgtatc
    60541 agacacaagg aactgattct tctgaatcat ctgaagaaaa aacagtgctg acagatatca
    60601 agatcactac caaagatgac gaatatatta tttcaggaaa agaaaataaa aaagaaacaa
    60661 cagtcatatt tacaaaaatc ggtaattacc gtttgaagga tgaggatggc aacatctaca
    60721 gtctatagtg ccgtctgttg gatcttcttt tgtttctccc cttcttttct gctatagtaa
    60781 agaagcaaga tgattgtaaa ggagacgaaa tggatgatcg atagtcctga ttattgtaca
    60841 tgtaataaca acaaaaatat ccatacagaa tttaatgacc aaggaagctt gagcatatgc
    60901 gacaactgtc acaaactagt caaagattca tttaaaacat ttgaatctta tcctgttcaa
    60961 aattatgtaa aaagtagaag aagataaaca aatccaagca aaactcacta tggctgattt
    61021 ttatgaactt caaactcagc ttcttccttt gatcaagctt catcagtaaa acaaacagtg
    61081 gatcatttca gtttacaaat caaagagtaa agatttattt atttcaaata tttgaatata
    61141 aaataaccaa acgatagaaa ggcttattct acatcacgta gtaatttttc tttcccgtcg
    61201 tttggttttt tatttttagc ctaattcttg accttttgca tgctggttat ccgtaaaata
    61261 ttttccagca ggctttcctt ccaattcagc taatttttct tggatctgac ggatcacctt
    61321 ttttgcatct tctggatttt ctacaaaatc gaatttatcc ccatccactt gtattttagg
    61381 acatacatca aaatcttcat accattgatt atagcgggtg ttcaacgttt cataataggt
    61441 atataacgtc tcatcgcttt ctagctgttc atagtcacgc ccacgttttt tgatacgttc
    61501 tagcatcgtg tccaaagaaa ctttgatatg gaccatcaaa tcaggccgtt tttttggagc
    61561 aacatcatct aactcgttta gcatcgtatc taataagcta tcgtactgct gtacttcgat
    61621 atccgttacc cgacctaaat cagcgtttag atggaacaac aggctatctt catagatgga
    61681 tcggtctagc acattgtcat cgttcactaa agcatttttc attgctaaaa atcgcttgtt
    61741 caaaaagaag atttgcaata aaaaagcgta ctgctcagga tttgaataaa agagcggcaa
    61801 tacttcgtta tcttcgacat tttcataaaa tggtttagaa tcaatctctt ttgcaagcat
    61861 atccgtcaaa ctgctcttac ctgcaccaat cgttcctgaa atcaaaatca ctgacataca
    61921 acccttcctc ttctataagt actcgcctac gtaagtttca acaggcaatg tttcaaaatc
    61981 atattcacgt acagcttggt ctgattttaa atacgtatgg agactttgtg taatcatcaa
    62041 gttcgtaggt accgtctctt cttgtacaat gatcatcggt tttttcaaca tagttgccac
    62101 acctaattcg taagccgttc ctggatcgat tgtttgatgc tcaaagtcaa ggatagcaac
    62161 aacaaattct gcatttgtca gttcttccat atctttttcg tatacttcct gcgcccatcc
    62221 agcgctgaat agttcataat tgctttcttg gtgatgacgt ggagagtaga aacttgtgac
    62281 tgttttgttt tcctccaacg ctttttcgat acgacttact cgatcgattt gctcctctga
    62341 aaaaaatggt cctgctaaat atatttgact cattattttg cctccttcaa taataactga
    62401 tttttaatag atactgctcg taattcattg accagttcca atggattgtt ggaaaagatt
    62461 acttgatcca accatttttt atccataaaa tcctcttttg caaaagtttc taataattgt
    62521 tgcaagccgc tgtaataacc gccaatattc aatattccga ttggcttgtc gggcgtcaga
    62581 ccgattgcta tccaagaaag aagttgtgag aattcctcca atgtacctag acctccaggg
    62641 aaaacgagga cagcatctcc cttttcaaat aataatacct ttctttcatc gatcgtttct
    62701 gtaggaataa atgtagtaac atcttttcca ggcaattcag tttcaaaaag cccattcgga
    62761 tagatacctg tgacttttcc gttattttcc agtactgttt tactaatcac tcccataatc
    62821 ccggagtatg aaccaccgta aaccagttcg atttcttctt gtgccatgta ttttcctaac
    62881 gtttgcgcaa taaatttgta actttcttta ttaccaaaac gggaaccgca aaaaacagta
    62941 agtttcataa aaaaacactc cctaactcaa aagtctatct tcattctaca cgtgtttttt
    63001 taaaataaaa gactagattt tgtagttttt tagtggtata taggatagac gccactatat
    63061 atagtattta gaaaaaaatc atttttcagc cattttatag ataaaacaaa aataaataac
    63121 gtgttttcat cgatatgaaa tgacttactt atagaagata ctgggatttc gtcctatatg
    63181 ccaataacgt gtattatttt aaacaacaaa tacttgtttc ttttattaac gaaaaaatca
    63241 ttttgttctc gtgttatgtt tcactgataa atgatgtttc tctaaataaa aaagccgacg
    63301 aactaaacga tcaaaatttg atcatttttg ttcatcagct tctcctaaaa atctttttgt
    63361 taacgataaa tagctggatt tgtaaaaata tcacttgaat cgtcactgct ggaagaaaat
    63421 tcagtgataa ccgcacctgt tgcgcctgcc tggaactagt gtttgatatt gggctcgaac
    63481 gtgtactgat ctctagcatg aagaataatt tcttttttta ctgtataata actttctttc
    63541 ctttttggaa tagtacagga aagttttttt gtttcctttc cttcgacata aggtatacca
    63601 gaccctttcg acaacgaaag gttctcattt ttccttcatc gtcatttcaa ggagggtgcc
    63661 gatgctctgg acagtttcga tcgctcctaa accaaaatct gtatattcga ttctttctaa
    63721 ctctgattct ttcataccat tcctgtctca aggtacatct attgtacttt tttcttgtaa
    63781 tccattctgc ctctcctaac taattgtcaa atcatcccaa gatatgtctt cttcctcttt
    63841 ttcttcttgg atgatttctg cattcggatc tactggtttt ttcaataata aaatggttat
    63901 tcctgtgaca aaagaaccag ctaacaatgc accaatgaat gcccatggtc tggtcatcgt
    63961 agcgactgca aacattcccg atgccggaat acggctatct gcgccccaaa acatgcttaa
    64021 tccgccacct actgtcccac cgactcccgt tgctatgaca ttgcgtgcca agtcgttcaa
    64081 tacgataggg aaacatccct ctgtgatctg acagatcccc ataggaaaag cggtcttcaa
    64141 tgtatcgatt tcttgacgat tgagtatatt tttacgaatg attttcccta agaaataagc
    64201 gatcgtcaag ccgaatgggg ttaccatcga agctaaaatc aatacagtga ttggttcata
    64261 gattccttct gaaaatcgtg ccacaccccc ttatccagaa taaatggtaa tccagaaaca
    64321 gccgtgtatc tgcggtaata agcctaacca aacgaaaaat atgagaagct atttttgttt
    64381 ggctgttctt attacttgat gcagggcagc tttttcacaa cctctttttt tatttatgtt
    64441 tcataatcaa tatggtaagg atgcagctcc gtatggtaga ccgcctccga atattcaatc
    64501 atttccccaa tttgcgtaga cgatttccgt atccgtttga tgccgatctg tgcgtctgtt
    64561 ttcagaattc cttgttcttc ttctgttaaa aatactgccg agaaatcatc agaaaatgag
    64621 tcaatagtat atcctttttt cgtcagcaaa cgatacaaag attctttgcc aaattcttct
    64681 ctgctgaatt gcatcatttc ttgtggaaga taatgaacaa aataaatata aggctgattg
    64741 ttcaaataat ataagcgctt aaaacaaaca gcttgctcac cgaaaaagga aaacaaagga
    64801 tcttccttct ttatatggac caattgcaaa tccagcgttt ctttccggac ttcatatcgg
    64861 gagttctcca agatttgcgt aaagcttgcc gctttggata atcgattgta aggacgattg
    64921 cttagaaccg tcgtcccacg accacttttc tttgcgacat attcatctga tgccagcagt
    64981 tcgattgcct ttcggacagt gattttgctg acttgaaaca gttcttctaa ttcactttct
    65041 gtcggcaaca tcgaaccgac tggatatttt cctgagaaaa tatcttcttt aattttatct
    65101 gcgacttcca agtatagtac acgtgtcctt ttcataacgt caccctttgc tttactatta
    65161 ctaaaagaat agcacataaa aaataaaaaa agaacacaaa tcacatgtga gaagtgtgtt
    65221 ctttttcatt cagctgcttt aattacaagt ttacgccatt tgaggcaatt acttctttat
    65281 accaatcaaa agattttttc tttgatcgat tcagtgtccc atttccttca tcgtctagat
    65341 ccacatagat aaagccgtaa cgtttactca tttctcctgt tgatgcactg acaagatcga
    65401 tacatcccca aggcgtatag cccatcaaat ctacaccgtc tgctactgca ttagccatcg
    65461 cttcgatatg acgacgtaaa taatcgatcc gataatcatc aataatcgtg ccatcctctt
    65521 ccatatgatc aatggctcca agaccgtttt caacaatgaa taatggtttc tggtaacgat
    65581 tgtataattc attcaatgcg attctcagcc cttctggatc gatctgccag ccccattcac
    65641 ttgcctcaag aaacggattt tttactcctc ccagcaagtt cccttctgaa gttgctgctt
    65701 gtggatcagt ctcattgatt gccgtagaca tataataact aaatccgatg tagtccactg
    65761 gatattcttt tagcaactgg aagtcttttt ccgtttgctc taatttttcc ggatcgactc
    65821 catatttttt atgggtacgc gctgtgtatg cagggtattc tccacgtact tggacatccg
    65881 tgcagaaaaa attgaattct tggttttgga tcattgttgc tgcctgattg actggattgg
    65941 catcgatgct gtaagttgtt gcatagataa tcatgcagcc gatttggata tccgaacgca
    66001 attcgtggcc gatttttacg gctttgctac ttgcaacgaa ttgattatgc catgcttgga
    66061 aaatattttg gtactctcct gctccattgc tttttactaa tccttgactt aaagctggaa
    66121 agtgaaaggc cgaattgatt tcattgaaag tcatccaata tttgacttta ctgctgtatc
    66181 tctctaatac tgtctgagca aatcgttcat agaagtcgat cagttttcga tttttccagc
    66241 caccgtattc ttttgccaaa tgaagaggca tttcgtaatg agaaatcgtg atcacaggtt
    66301 caatatcata ttttaagcac tcatcaatca gctgatcata aaattctaat cccgcttcgt
    66361 taggagtacc ttcgtctcca tttggaaaaa ttcgtgtcca ggcaatcgaa aaacgataac
    66421 atttgaatcc catttccgca aacaaggcaa gatcttcttt gaaacgatcg taatgatcga
    66481 ttcctcgatg attcgggtaa atatattttt cttgatcaat cgtccagtca aattcttcac
    66541 tgccgataat cgcaaagcgc tgcttacctc caggcatagc atctgcaacg gatagtcctt
    66601 ttccgtctcg atcataagcc ccttctaatt gatttggcag cagttgctcc accccataaa
    66661 aatttttctg aaaaacgtga ttgcatcctg atttcctcct tattttacaa ttgccagaac
    66721 atcttcgttt gcaataatct ctttttggtc taattcaagt atatcggcaa atgcatcagt
    66781 attcgtaatg acaactggag tgattacgct atagccagct gctctaattg cttcgatgtc
    66841 aaaacgaatc aataaatctc ctgctttgac gagatcccct tgtttgacta agatctcaaa
    66901 gccattccca tttaattcta ctgtatccat cccgatatga atcaaaatct ccgctccgtc
    66961 agtcgttgtg atccctacag catgtccagt tggaaaaata gtcgtgatct ctccgtctgc
    67021 aggtgcaact agttctccta cttctggatc aatagcgata ccttttccca tcacgccaga
    67081 agaaaaaact tggtctggta cttcactcaa tggcagtact ttacccgtta aaggactttt
    67141 taaggtatgc cgtgctgaag taatcggttc tcctgcattc gcatgtttgt tttctaattg
    67201 attgtcttgt gtttgttcgt caaatcctaa aatcaatgta cctacgaatg caagaacaaa
    67261 agcaattcct gtagctatta ctgctactgt cacatttgac tctacaccat ccaccgtatt
    67321 aatgaaagtc ggaatcgtca ataagctgac caaactgctg gagaaagctt ttactccgga
    67381 aaatccgata atagctccac caatacctcc agaaatacaa gcggcaatga atggtttctt
    67441 caaaggtaat gtcacaccat acacagttgg ttcagtgatt ccaaataacg aagtcactgt
    67501 agaagaaatc cctaatgctc gaagtttcgt atcttttgtt cgcaatgcta cagctaaagc
    67561 agccccacct tgtgccaaga tggctggaag aagcattggc atcaatacat caaacccata
    67621 ttgttcgata ttcaaaaaca tgatcggtac gaatccccaa tgcatgccga acatcacgaa
    67681 tacttgccat aaacttccca tgattagacc tgctacgatt ggactaaatc cataaatcga
    67741 gttgaagaga ccgcctaaag cattaccggc aaccgtcccg attggtccta aagcaagaaa
    67801 tgttaatgga accatcacta ctaaaacgac aagcggaact aaaatcattt gcagaaattg
    67861 cggaatcact ttttttacga atggctctaa tttactttga acccatacgg ctagaatgat
    67921 tggtatgaca gaagaagtat aaccagatgg tgaaagaatc actgggatcc caaagaaact
    67981 gatagcttct cccgcaccag caactgctgt gatagctgga tacacaagtg ccatagcaat
    68041 tgctactgct aaaaactggt tggctttgaa ctttttagct gctgtaaaag caagcatgac
    68101 aggtaagaag gtaaagacac catctgctgc agcaaacaat atttgataag taccgctatc
    68161 agctgtcagc cagcccatca ccgttgccaa tgataagaat cctttcaata ccccggcggc
    68221 tgccatcgcc cctagaaaag gtgtaaatat actagagata atatcgatca gacgattcaa
    68281 aatatttttg gactcttctt ttccatcctc cgaaactgca agaccttcct catccacgat
    68341 cgattgataa acatctgcta cattactgcc aatcactact tgatactgac cgccactttg
    68401 tacgacttgg atcacgtccg ggtcttcttt taacttttgc gtatctgcta aagattcatc
    68461 ttttaatctg aatcgcaacc gcgtagcaca atgtactaaa cttttgacat tcttttgtcc
    68521 tcctaccgct tcccatacac gacgaccaac tgcttgatta tccattgttt tttccttctt
    68581 tcttttttat ttaaaaaata aaaacccaga ttgaaaaaaa cagtcacaaa cattcgtgaa
    68641 cctatttttt caatccaggt tttgcctgcc atacagtaac aatccttatg attggatgct
    68701 ttttttctca cttactactc tttgaatatg aatcgttaaa taaatctgct ctgcgatccc
    68761 catttcctgt ttttgcgttt gtcgaagata ctcattgatc cgttcgacac aatgaaacgc
    68821 ttttggatac tttccttgaa ctaatgcgta gagaaattca tcttgctcgt cagatccttc
    68881 ccctttgaga taacgctggg caaaaaactg cagatgagta atgatacgct gataattcaa
    68941 agattcttca tcgaacacga tgccgaagaa tttactgatg atactcagta tatctcttac
    69001 cattgtggta gcggtcatcg tcacttgcat gtcttcgttt ccttgttggc tattcacaaa
    69061 atgaaaggca ataaaaccgg cttcatcctc agaaagttcc acacccgtct tttgtttcac
    69121 gaacaaaaga gcctctttcg ctgcttgata ttctttcgga taaaattttt ttgtttcaaa
    69181 taataatgga ttaggcagtt ggatattttc ttttgctcgt gtgatcgcat aatgaagatg
    69241 atcggtcaat gtcagataaa tattaggcga aaattcaact tttagtttat cttcagccag
    69301 ttcgactact tgtttgaccc attcgatctc ctctgccgga atatcagcca tcagctgctg
    69361 gaattggttt
//
LOCUS       DAYLZO010000003        66452 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.003, whole genome shotgun sequence.
ACCESSION   DAYLZO010000003 DAYLZO010000000
VERSION     DAYLZO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.003"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(445..744)
                     /locus_tag="ACGVJG_000139"
     CDS             complement(445..744)
                     /locus_tag="ACGVJG_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFJ6556129.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            1095..1727
                     /locus_tag="ACGVJG_000140"
     CDS             1095..1727
                     /locus_tag="ACGVJG_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFJ6556130.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1850..2488
                     /locus_tag="ACGVJG_000141"
     CDS             1850..2488
                     /locus_tag="ACGVJG_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFJ6556131.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(2607..2679)
                     /locus_tag="ACGVJG_000142"
     tRNA            complement(2607..2679)
                     /locus_tag="ACGVJG_000142"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2644..2646),aa:Thr,seq:cgt)
     gene            2800..3042
                     /locus_tag="ACGVJG_000143"
     CDS             2800..3042
                     /locus_tag="ACGVJG_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556132.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(3181..3810)
                     /gene="upp"
                     /locus_tag="ACGVJG_000144"
     CDS             complement(3181..3810)
                     /gene="upp"
                     /locus_tag="ACGVJG_000144"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFJ6556133.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3944..4891)
                     /locus_tag="ACGVJG_000145"
     CDS             complement(3944..4891)
                     /locus_tag="ACGVJG_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFJ6556134.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(5044..6288)
                     /gene="glyA"
                     /locus_tag="ACGVJG_000146"
     CDS             complement(5044..6288)
                     /gene="glyA"
                     /locus_tag="ACGVJG_000146"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFJ6556135.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(6479..7504)
                     /locus_tag="ACGVJG_000147"
     CDS             complement(6479..7504)
                     /locus_tag="ACGVJG_000147"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFJ6556136.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(7526..7705)
                     /locus_tag="ACGVJG_000148"
     CDS             complement(7526..7705)
                     /locus_tag="ACGVJG_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556137.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(7698..8537)
                     /gene="prmC"
                     /locus_tag="ACGVJG_000149"
     CDS             complement(7698..8537)
                     /gene="prmC"
                     /locus_tag="ACGVJG_000149"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFJ6556138.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(8530..9603)
                     /gene="prfA"
                     /locus_tag="ACGVJG_000150"
     CDS             complement(8530..9603)
                     /gene="prfA"
                     /locus_tag="ACGVJG_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFJ6556139.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(9606..10190)
                     /locus_tag="ACGVJG_000151"
     CDS             complement(9606..10190)
                     /locus_tag="ACGVJG_000151"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFJ6556140.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(10296..10544)
                     /locus_tag="ACGVJG_000152"
     CDS             complement(10296..10544)
                     /locus_tag="ACGVJG_000152"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6556141.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            complement(10535..10765)
                     /locus_tag="ACGVJG_000153"
     CDS             complement(10535..10765)
                     /locus_tag="ACGVJG_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556142.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            10871..11473
                     /locus_tag="ACGVJG_000154"
     CDS             10871..11473
                     /locus_tag="ACGVJG_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFJ6556143.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(11577..12146)
                     /gene="lepB"
                     /locus_tag="ACGVJG_000155"
     CDS             complement(11577..12146)
                     /gene="lepB"
                     /locus_tag="ACGVJG_000155"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFJ6556144.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(12376..13143)
                     /locus_tag="ACGVJG_000156"
     CDS             complement(12376..13143)
                     /locus_tag="ACGVJG_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFJ6556145.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(13140..13619)
                     /locus_tag="ACGVJG_000157"
     CDS             complement(13140..13619)
                     /locus_tag="ACGVJG_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFJ6556146.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(13744..14481)
                     /locus_tag="ACGVJG_000158"
     CDS             complement(13744..14481)
                     /locus_tag="ACGVJG_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFJ6556147.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            14642..16042
                     /locus_tag="ACGVJG_000159"
     CDS             14642..16042
                     /locus_tag="ACGVJG_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFJ6556148.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            16265..17617
                     /locus_tag="ACGVJG_000160"
     CDS             16265..17617
                     /locus_tag="ACGVJG_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFJ6556149.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            17610..18308
                     /locus_tag="ACGVJG_000161"
     CDS             17610..18308
                     /locus_tag="ACGVJG_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFJ6556150.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(18370..19521)
                     /locus_tag="ACGVJG_000162"
     CDS             complement(18370..19521)
                     /locus_tag="ACGVJG_000162"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFJ6556151.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(19534..19971)
                     /locus_tag="ACGVJG_000163"
     CDS             complement(19534..19971)
                     /locus_tag="ACGVJG_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFJ6556152.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19986..21752)
                     /locus_tag="ACGVJG_000164"
     CDS             complement(19986..21752)
                     /locus_tag="ACGVJG_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFJ6556153.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            22016..22693
                     /locus_tag="ACGVJG_000165"
     CDS             22016..22693
                     /locus_tag="ACGVJG_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFJ6556154.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            22696..23823
                     /locus_tag="ACGVJG_000166"
     CDS             22696..23823
                     /locus_tag="ACGVJG_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6556155.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(24023..24463)
                     /locus_tag="ACGVJG_000167"
     CDS             complement(24023..24463)
                     /locus_tag="ACGVJG_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFJ6556156.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(24474..26507)
                     /locus_tag="ACGVJG_000168"
     CDS             complement(24474..26507)
                     /locus_tag="ACGVJG_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFJ6556157.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(26509..28212)
                     /locus_tag="ACGVJG_000169"
     CDS             complement(26509..28212)
                     /locus_tag="ACGVJG_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFJ6556158.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(28306..29256)
                     /gene="manA"
                     /locus_tag="ACGVJG_000170"
     CDS             complement(28306..29256)
                     /gene="manA"
                     /locus_tag="ACGVJG_000170"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFJ6556159.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(29295..30320)
                     /locus_tag="ACGVJG_000171"
     CDS             complement(29295..30320)
                     /locus_tag="ACGVJG_000171"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFJ6556160.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(30529..31398)
                     /locus_tag="ACGVJG_000172"
     CDS             complement(30529..31398)
                     /locus_tag="ACGVJG_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFJ6556161.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMINFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(32102..33994)
                     /locus_tag="ACGVJG_000173"
     CDS             complement(32102..33994)
                     /locus_tag="ACGVJG_000173"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556162.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33991..35718)
                     /locus_tag="ACGVJG_000174"
     CDS             complement(33991..35718)
                     /locus_tag="ACGVJG_000174"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556163.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(35734..36384)
                     /locus_tag="ACGVJG_000175"
     CDS             complement(35734..36384)
                     /locus_tag="ACGVJG_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFJ6556164.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            36588..36884
                     /locus_tag="ACGVJG_000176"
     CDS             36588..36884
                     /locus_tag="ACGVJG_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFJ6556165.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            37076..37696
                     /locus_tag="ACGVJG_000177"
     CDS             37076..37696
                     /locus_tag="ACGVJG_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFJ6556166.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(37788..38954)
                     /locus_tag="ACGVJG_000178"
     CDS             complement(37788..38954)
                     /locus_tag="ACGVJG_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFJ6556167.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38966..39319)
                     /locus_tag="ACGVJG_000179"
     CDS             complement(38966..39319)
                     /locus_tag="ACGVJG_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFJ6556168.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            39447..40469
                     /locus_tag="ACGVJG_000180"
     CDS             39447..40469
                     /locus_tag="ACGVJG_000180"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFJ6556169.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            40488..40649
                     /locus_tag="ACGVJG_000181"
     CDS             40488..40649
                     /locus_tag="ACGVJG_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556170.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(40908..41342)
                     /locus_tag="ACGVJG_000182"
     CDS             complement(40908..41342)
                     /locus_tag="ACGVJG_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556171.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(41452..43311)
                     /locus_tag="ACGVJG_000183"
     CDS             complement(41452..43311)
                     /locus_tag="ACGVJG_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFJ6556172.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            43312..43452
                     /locus_tag="ACGVJG_000184"
     CDS             43312..43452
                     /locus_tag="ACGVJG_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFJ6556173.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(43429..44589)
                     /locus_tag="ACGVJG_000185"
     CDS             complement(43429..44589)
                     /locus_tag="ACGVJG_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFJ6556174.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(44896..46320)
                     /gene="araA"
                     /locus_tag="ACGVJG_000186"
     CDS             complement(44896..46320)
                     /gene="araA"
                     /locus_tag="ACGVJG_000186"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFJ6556175.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(46336..47034)
                     /locus_tag="ACGVJG_000187"
     CDS             complement(46336..47034)
                     /locus_tag="ACGVJG_000187"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFJ6556176.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46991..48652)
                     /locus_tag="ACGVJG_000188"
     CDS             complement(46991..48652)
                     /locus_tag="ACGVJG_000188"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFJ6556177.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            48870..50270
                     /locus_tag="ACGVJG_000189"
     CDS             48870..50270
                     /locus_tag="ACGVJG_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFJ6556178.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(50522..51601)
                     /locus_tag="ACGVJG_000190"
     CDS             complement(50522..51601)
                     /locus_tag="ACGVJG_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6556179.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(51687..52646)
                     /locus_tag="ACGVJG_000191"
     CDS             complement(51687..52646)
                     /locus_tag="ACGVJG_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFJ6556180.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(52658..54169)
                     /locus_tag="ACGVJG_000192"
     CDS             complement(52658..54169)
                     /locus_tag="ACGVJG_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFJ6556181.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(54191..54755)
                     /locus_tag="ACGVJG_000193"
                     /pseudo
     CDS             complement(54191..54755)
                     /locus_tag="ACGVJG_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(54761..55624)
                     /locus_tag="ACGVJG_000194"
     CDS             complement(54761..55624)
                     /locus_tag="ACGVJG_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6556182.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(55638..56534)
                     /locus_tag="ACGVJG_000195"
     CDS             complement(55638..56534)
                     /locus_tag="ACGVJG_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6556183.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(56709..57998)
                     /locus_tag="ACGVJG_000196"
     CDS             complement(56709..57998)
                     /locus_tag="ACGVJG_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6556184.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57970..59487)
                     /locus_tag="ACGVJG_000197"
     CDS             complement(57970..59487)
                     /locus_tag="ACGVJG_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFJ6556185.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            59685..60575
                     /locus_tag="ACGVJG_000198"
     CDS             59685..60575
                     /locus_tag="ACGVJG_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFJ6556186.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(60648..61472)
                     /gene="nadE"
                     /locus_tag="ACGVJG_000199"
     CDS             complement(60648..61472)
                     /gene="nadE"
                     /locus_tag="ACGVJG_000199"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFJ6556187.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(61484..62956)
                     /locus_tag="ACGVJG_000200"
     CDS             complement(61484..62956)
                     /locus_tag="ACGVJG_000200"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFJ6556188.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(63593..63955)
                     /locus_tag="ACGVJG_000201"
     CDS             complement(63593..63955)
                     /locus_tag="ACGVJG_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFJ6556189.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            64178..64930
                     /locus_tag="ACGVJG_000202"
     CDS             64178..64930
                     /locus_tag="ACGVJG_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFJ6556190.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(65070..65993)
                     /locus_tag="ACGVJG_000203"
     CDS             complement(65070..65993)
                     /locus_tag="ACGVJG_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6556191.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(66008..66280)
                     /locus_tag="ACGVJG_000204"
     CDS             complement(66008..66280)
                     /locus_tag="ACGVJG_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556192.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        19254 a        13589 c        11498 g        22111 t
ORIGIN      
        1 ctcaaaaatc ctttcttcaa ttcctccccc actcatttcc aatcttttaa ctaaaaaatt
       61 gatagttatt ttacaaatat ttataaaaaa taaatatgcc aaaaaactgt ttttacaagg
      121 tttttcctaa tgtaaattat tttttcgcat atattcattt tattttactt attgctttca
      181 ttttttcaat ctatatttcc aatttttaac ttacaaaaat gcaacattaa taaataacga
      241 tagtttcata aaatacactt ttaattaaca ttattagttt atactagctg tttttgtgtt
      301 aattaaagga taaaatacaa tataaagaaa catctgtttc tttatttaga ataaaaaaat
      361 acagactctg ttcaaataaa tgaacctagc ctgcattatg agattgacta tactttttaa
      421 ttcattgaga tgaataactc gtttttataa atgcccaaaa gtaatcggtg ccgaagccat
      481 tcctcgacct aattcttttt caaattttcc tattgttgat atctccatat aaaacggtga
      541 tactggttta gacaattctc ccctgattgc taacggacgt aaactacgtt ccaaattatt
      601 tatgacttta ggataatact gacctttttc taagcctgtc tttgcgtcta tcaaaagtat
      661 acgttccttt tctttgatct ctggatttaa aacgagatca tataatttta gtaccatgtt
      721 ttctatttct tcacttttag acatcacgat ttcctcccaa aaatatattt cttcccacta
      781 cattctataa acaaagtata cgctacatct agattttttt aaatcattac tggattgtta
      841 aaacctttca atacactgtt atggcaacga tttttatttt aaaaacattc taaattaaat
      901 cagtaaaaat ttttattcta agatttttat tagaattacg cctaatttgt gtctctcagg
      961 gcaattctaa gaacgtttca agagatagcg acactatcgt tatcgaaaaa ggcgaaaacc
     1021 tatctcattt tctgctaatg taagttggtt tagcttttgt taatatgact ttatgaaaat
     1081 ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc aagcttgaat aaaggatcct
     1141 tttttcatat ctatgaaaac gaagattttg aactgtataa tatcctttca caaaaagagg
     1201 tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa atctattttc aagtatgcat
     1261 tttttgcttc caatgatttt caaagaatgg agattaccga ctgtatattc gaaaactgtg
     1321 atttttcagg ctgtatattg acaaaaagca tgttgcatcg cgtgaaattt gtcaattgta
     1381 aattaacagg tactaaattg atggaaagtt atattgggaa cacgttattc gagaattgta
     1441 aaatggacta tgtaaattta tctggttcaa acataaaaga aagtaccttt gagcactctg
     1501 tcttatcaag cgcagatttt gtagactgtt ccctcactaa aataatgttt tctactaatg
     1561 atttagaaaa tgctagcttt tttgatactg atttaaaaaa tatcgattta agtagcaact
     1621 cctttgaaat gattgaagta cgctcggaac atctaaaaaa ttgtacgctt aatcagttgc
     1681 aagcacttgg ttttgctaga aaatttttac aaataaaagt agtataatca aaatgaatat
     1741 ataagataaa tttttagcaa tacaaatatc cataaaaaaa cattgcctat tttaactctt
     1801 catgtatact gactctatct tcaaaaactg aatagtagga gttattatca tgaataacat
     1861 tttaggaaaa aaaatagaac ttaaaaaagg gatattgggc actattgtcg ttgtactcgg
     1921 tttgttaggg ttgctatttg cgccatccat tattagcatt ttttctttaa ctatcactgg
     1981 atttatagca tttacacttg tttttggtaa aaaagatacg aaaaaaatgt tctcgaaacc
     2041 tgtcgctcct gtcaaaaata tagccaaata tttttttatc aatgtagtaa tcagtgccgc
     2101 agtctcggta tttttgcaac aaattttgaa atggggttta acaggcaatc caataaatga
     2161 ggctttcagc ccgttattat ttattatttt gcctatcatg atacttggag aagaattgtt
     2221 ctcagtgtat tttctcgcta ttttttctag taagttcagc attcctgtcg ctagtttctt
     2281 gtcagcaatc atctttggat ttatccatta cagtacttat gataatggaa atattttaca
     2341 cactgtagca cacatccttt tgatacaagg ggtggcaaga ttgttattca accaagctgc
     2401 cattaaaagc aactcgatca ttacaagttg ggcagttcat gtgatatttg atttcacagc
     2461 tatattgcta gtcgttttat tctcctaaat ttttatagaa ataatacatg ctctattgct
     2521 aagaaagcat actttatttc ctttaaacaa aaagacagat gtatcaatgg attaaaaatc
     2581 cttgatatat ctgtcttctc ttttaatgcc agctataggg atcgaaccta cgacctacgc
     2641 gttacgagtg cgttgctcta ccaactgagc taagctggcc ttatccaaaa gaaattatat
     2701 actctatttg gctgattgta aagagtccga tggaaaatta gtttaatttc atgatatttt
     2761 tcataaaacg tggtataatt gatgtgaaag gcaggtttta tgaaaatggc aacttttgga
     2821 aaatttgata caactatcgt accatcggaa attggtgaaa aattcaagat caatactcac
     2881 gtaatgttca caattcacgg caagtcgcaa actggaacga tttcaaagca attgaaaaat
     2941 tccgctgttg ttgagattga tgaaactgca gaaaatgcga acttgatgac gcaaagtaag
     3001 ggtgtagtaa tcgttaacta caaagaacta aaaaagattt gaatttgacc atctctgcat
     3061 ttttgggatg gtttttttat atgcaaacga aagctgtgaa aaagctgttc tgcatcaaac
     3121 gttaaaaatg accaaaaaag gatgtgacaa aagttgtgtc acaccctttt tcgtatttac
     3181 ttattttgta ccaaacaaac ggtcacccgc atcccctagt ccaggaacga tataaccatg
     3241 ttcattcaat ttttcatcta gagaagctgt atagatgtca atatcaggat gcgcttcttg
     3301 caaagctttt actccctcag gtgctgctac caaacaaaca aatttaatgt tcgacgcgcc
     3361 acgttctttc aatgcatcga ttgccatgat tgctgatccg cctgttgcca acattggatc
     3421 tactacaaac aactgacgtg catcaatgtc ttctggaagt ttgacgaaat attcatgtgg
     3481 ttccaatgtt tcttcatctc gataaagacc gatatgtccc actttagcag ctggaatcaa
     3541 ttccaagata ccgtcaacca ttccaatacc tgcgcgcaag attggaataa tcgctacttt
     3601 tttaccagac aacgtttttt gtgttgtttc agtgatcggt gtttcgatca cgatgtcttc
     3661 caatggcata tcccgtgata cttcatatgc catcaacata gcgatttcat cgactacttc
     3721 acgaaaaacc tttgttccgc agtttttgtc ccgaataata gttagtttgt gttgaatcaa
     3781 tggatgatcg atcacttgaa atttgcccat tttttaagct ctcctttgtt ctgtcgtttc
     3841 tttttaattg taaaacaaaa tcaagtgatt gactagtcaa actcgccatt tttcctaaga
     3901 aaatccgttt ttgaaaagaa gttttcctgc ggacaagcaa agcttattct tttttcttga
     3961 tccaagaagt agataatgcc attgctgcac gcgttgcctt tgtctgatcc atagcattta
     4021 ccatgacaaa atcatggatg attccttgga agcgtacttg tgtaacagga acccctgcat
     4081 cacgaagttt gcgcgcataa gcctcacctt catctcttaa tacatccgct tctcccgtta
     4141 aaatcattgc tgaaggaaga tcatttagtt catttatatt tgctctcaaa ggcgaagctg
     4201 tgatttcagc acgctcttct tcattcgtcg tatattggtc ccaaaaccac atcattcctt
     4261 ctcttgtcaa aaaatagttt tctccgaatt catgatagga atctgtatca aaatcagcgt
     4321 ttgtgactgg ataatacagc aattgcttgg atattgggag gccctttcgt tcttttgtta
     4381 agatcgtcat gactgtagcc atgttaccac ctactgaatc tcctgcaacg attagattct
     4441 cgagatccag attttttgac ttggctaatt tattcagctg ttgcaacaca gcataatttt
     4501 gttcaattgc tgttggatat tttgcttctg gtgatcgact atattctgga aagacaacga
     4561 cggaattcgt acgaaccgca agttctctga tcaatttatc atgggtttga gcactgccaa
     4621 aaacccagcc tgctccatga atataaaaaa taactggtaa tttatccata ttcccttctg
     4681 gtcgtacgag acgaatattg agttttcccc attcgccagt atccaccatc aagtcttcaa
     4741 tatcgacgga atatttgttc acaggtgatt cttgtacttc ttctagtaaa actcttcctt
     4801 cttccggtgc tagttcatag atacgcggat gaggcgcatt tgcatcactg aatgctactg
     4861 cttccttttc taacgagata cgatcagtca tgacttttcc tccctgagat ttagtgtgaa
     4921 gactaaaatt acacctcata tactagagag catcaccgat attgtcaaag aatccgtttt
     4981 cccaaaaaat aaacagaaaa gcccaacatt gaacttttct gtttactaat ggaatttaaa
     5041 tttttatttt ttatgcaacg gaaatttttc agttaattcg cgtacacctg ttttgacttc
     5101 gtctagttga gcgttatcat cttttgcttg taatgctttg acaactagtt cagccacttt
     5161 tacagcatct tcttctttga acccgcgagt agtgattgct ggcgtgccga ttcgaatacc
     5221 gcttgttttg aatggactta gtgtttcaaa aggaatcgag tttttattta cagtgatatt
     5281 cacactgtcc aaaatgctct ctgcttcttt tccatttata cctaactcgc gaacatcgat
     5341 caacatcaga tgattatctg tcgcccctga aatgactctg gttccaattg cttgattgaa
     5401 aactttgacc attgcttttg cattggcgat gatttgttca ctatattctt taaatgcagg
     5461 atctaatgct tctttgaatg ctactgcttt acccgcaatc acatgctcta gagggccgcc
     5521 ttgaatacca ggaaataccg cactatttat tttttttgct aatgcttcat cgtttgtcaa
     5581 aatcatgccg cctcgagggc cacgcaatgt tttatgagtg gtagtcgttg tgatatctgc
     5641 ataaggaact ggattaggat gaagtcctgc tgctactaat cctgcgatat gtgccatatc
     5701 taccatcagt tttgcgccta cttcatctgc gatttctctg aatttcgcaa agtcgatcgt
     5761 tcgtccgtaa gcactggcac ctgctacgat caattttggt tgatgttttc tagctaaaat
     5821 acgtaccaca ttataatcaa taacttctgt tgtcggatca acaccgtaag caacaaagtg
     5881 ataggttttg ccactgaagt tgactggtga tccatgtgtt agatgtccgc ctgcggaaag
     5941 atccattcct aagatcgtgt cccctggttc taccaaagcg agataagcag ccgtgttggc
     6001 ttgagagccg gagtgaggct ggacgttcgc aaatttcgct ccgaataatt cttttgcacg
     6061 gtcaatcgct aaattttcaa cgatatcgac aaattcacac ccaccataat agcgatgccc
     6121 tggataacct tctgcgtatt tatttgtcaa gatacttcct tgtgcagcca tcactgcttc
     6181 ggagacaaag ttttcagagg caatcagctc caaattatgt tcttgtcttt cttcttcttt
     6241 ggcgatcgca gcccaaagat cagggtcaaa cgttttgtaa tctaccacaa acaacagctc
     6301 ctttttattt tcctacaatt ttattttcct acaattttat ttttcccatc aaaaacaaat
     6361 tgccaaatat tcgtatctct tctattacga ttagttacgc ttagtttttc gttcactgat
     6421 agaagattta cttgatagca ttgtatcata ctaaaagaat gcgacaataa ccaattgatc
     6481 atttttcaaa ataattttgt cctgctgctt ttttcaagcg attcatgtaa gcattcccca
     6541 atccagtttc tggatacact tgtacaaaaa tcaaatctaa tccaagtgtt ggttcatcta
     6601 atcctcttag acctgcaaac aatcctttag ccgccgcttc tactgagttg tcgttataca
     6661 tataaacagc tgcagtatct gtccgcactt gatccgcaat ttcggggctt gctatgacac
     6721 ctgcacggat cttcttattt tttgcccatt gaactgcagt tgaccaatcg ccatctctga
     6781 ccatcagaac acgtgtatcg ggtgagtagt gtttgtattt catcccaggg gcttttggtg
     6841 tttcattttc ttttacaaga tgctgatcaa tagcaacagg agattcgatg actgcttcga
     6901 tttgttcttt cgttactgca cctggtcgta aaatcatagg catggctgtt ggatcactta
     6961 aatcaagaac agtggattct acaccaattc gcgtagctcc atcatcaata atcccggtaa
     7021 tttttccttg tagatcatga tatacatgat ctgcagtcgt tggacttggt tttcccgacg
     7081 tatttgcact tggaccgaca agcggaacac cggaaagttc gattacttct agtgtttttt
     7141 tgttatccgg tatacgaaat gcagctgtgg ataatcctcc cgtcacaact gagggcaaag
     7201 tgtctttttt gatcttaaat atcaaagtta atggtccagg ccagaagttc ttcacaatct
     7261 tctctgctgc cggatgaaag ttgtccacat actcttttac ttgttcaaaa gaggcgacat
     7321 ggacgattaa tggattatct tgcggtctcc cttttacaga aaacactttc ttgacagcgt
     7381 ttggtaaaag agcgttggcg cccaatccgt atactgtttc ggtaggaaaa gcaacaagtt
     7441 ctccttcttt cagtgcttcg gctgcctctt gcaattctgt ttcatcatat cgtttcgttt
     7501 ccacagttta tccacaaccc ctttttcata cattcttatt ttgtctgagt ttatcctcaa
     7561 gttttccact gtttgtggat aatctatttg tcaatagtgg ataaacttgt tgataaacca
     7621 aaaagtcgtt tacatccttg tcaaataagt gtttttttat aataaaaaaa caaataaaaa
     7681 atttttcatc cacaacttca accataaact tggatcattc gttcattgcc aaaaagatcc
     7741 ttttttatcg ccactttttt atctggaaat gcttgctgaa atattttttt tactgcttca
     7801 ccttggcgaa atccgatttc taaaaaaatc tttccatttg attttagtac gcttggtgct
     7861 tcaactgcta ttttttcata aatagctaat ccatcgttct cagcaaaaag ggccatttta
     7921 ggttcgaatg accggacact ttcatccatc agttcccatt cattccgact gatgtacggc
     7981 ggattagaaa taatcacatc aaattgctga tccattactg gtcctaatgt atctccatga
     8041 taaaaactga ttttcgttcc ttctttctga gcattttctt tcgctacttc taatgcttct
     8101 tctgaaagat ccacagcaga tatatgccaa ttttttcttg ctgctttcaa actaatagca
     8161 attgctccag tccctgtccc aatatctatc acttctaacg gtacaccagg cgtctcgtct
     8221 aaacaccatt cgactaactc ctctgtttct ggtcgaggga tcagtgttgc ttcagttacc
     8281 tttaaccgat gatcataaaa atctgcatat cctagcaaat actgaggtgg atgatctgca
     8341 agcagaagtc gcagatcggt ttcgatcaac cgctgttctt cttctgaaat ctcttcgttc
     8401 atatgaagga gccaatcaag ttttttccat tgttttcgtt ccaaaaataa aaattggata
     8461 ctatatgctt ctttcccatg atcctctaaa aaagaagaag cccgtgacag gacttctcga
     8521 taggtcattt tacccatttt gcatagcttc cagttttgat gtttgatcat acatgatcaa
     8581 tgcatcaacg atctcatcta atttccctgc taaaatctga tctaattttt ggatagttaa
     8641 tccgatacga tgatcagtca cacgattttg cgggaaatta tacgtccgga tccgttctga
     8701 acgatcccca gttccgactg ctgattttcg attggcatca tactcgcttt gtgcttcttg
     8761 ctggatctta tcgtacacac gagcacgcaa gattttcatt gctttttcac ggttcttgat
     8821 ttgagaacgt tcatcttgca tagcaacaac gatccctgtt ggtaagtgag tcaaacgtac
     8881 agcagatgct gttttattga cgtgctgtcc gccagcacct gaagcatgat agatatctgt
     8941 acggatatct ttgtcttcaa tatcgatctc tacttcttca gcttctggca tcacgacaac
     9001 tgttgcggtg gaagtatgga tacgcccttg cgattcagtt gaaggaacac gctggacgcg
     9061 gtgcgcacca ctttcatatt ttaatttcga atatacgttg tctccggtga tcatcatgat
     9121 cacttcttta tatccaccga ttccggtaac gttagcttcc atcacttctg ttttccagcc
     9181 ttgtgcttca gcatactttt ggtacatatt gaaaagatcg ccagcgaata atgctgcttc
     9241 gtctccgcct gctgctccac ggatttccat gatgatattt ttatcgtcat ttggatcttt
     9301 tggtaaaagc aagatcttga ttttttcttc taagacttct ttttcttttt ttaattcaga
     9361 taattcttct tttgctagtt cagccatttc atcatccagt ttttcaccaa gcagttcttc
     9421 tgtatcggtg attccttgta ctacttcttt ataacgacga taaacttcga ctgtttcgcg
     9481 cgtgtttgct tcttcttttg aaagctgcat aaaacgcttt gtatcagaaa tcacttctgg
     9541 gtcacttaaa agttctccaa gctcttcata acggtcttca attgcttgta attgatcgta
     9601 catgcttatt ctccttctgt aatcattgat gggtgaaagt aatgttttcg acaaacgggg
     9661 taataagctt catttccgcc aatttgcact tgatcgcctt cataaacagg gtttccatca
     9721 atataatgta aattcatgat tgcttttttg tggcagaacc aacagattgt tttcatttct
     9781 tcgattttgt ctgcataaag caataaatat tttgatcctt caaataactc gtttcgaaaa
     9841 tcatttttca aaccaaatgc cataaccgga atatccaatt cgtccacaat tctcgtaaaa
     9901 gcaatgacat gtttcttttt taagaattgc gcttcatcga tcagcacaca atagggtttg
     9961 ctttccattt tctgaattac gtcaaatacg tctgtttctt caaatatcgg catcgcatta
    10021 cgtttcagtc cgattcggct tgaaacaacg cctaaaccat ctcttgtatc aataccgctt
    10081 gtcattaaga caactggctt attttgttct tcataattat gtgcgacttt taggatctcg
    10141 atcgtcttgc cgctattcat tgcgccatac ttaaaaaata gttgtgccat gctctttttc
    10201 accttccagt ttgttcctat ggtattatac ttgattttca gcatttttta cagtgagaat
    10261 tttcatcaag cacagattag tattaaatct attgctcact tttgttccaa ttttttgaca
    10321 agaactaaac atgggttcca ttcatcccat aaatttggaa aaacttccaa ccttgaaaat
    10381 cctaatgatt cgtagaaaca aatagtttga tcatatatag agtagtgccc ctcgtctaca
    10441 gtcttcactt gcaaatattt gtaatgtttt gctgcgtaac cttctgcttc tttaaaaagt
    10501 tttgttccta atccttggtg atgatagtct ttttttattc ccatacaata gaatagattt
    10561 cggcagtatc ttcacttgtc tgtgaaagag tgataaagcc taaatattct tcttttcttt
    10621 tttcacacca taaaggtagt ttacttgatt cctcgatata tttttccgtt gattcaggaa
    10681 gcccaaacca ctcaggcaag tctagtaata tttctcggac acactttcct ttttcctctc
    10741 tatcttttac aaacgatatc ggcatattct ttctagattc tccctttttg aattgagtat
    10801 agcatgcttt ctttcttttc acgtgctata ataagttaaa atttcaaagt tttacgtaag
    10861 gagctatttg atgacaaaaa atatttttga ttccatcgct aaccattatg attcccctga
    10921 ccgtcaagca ttagccaaga ttattcgcga agaactaact acttatctac cgcgcgacag
    10981 ccaccaaaaa gtcctgctgg attacggagg cggaactggg cttgttagtt taccgcttgc
    11041 ggaaagattc aaagaactga tcattgccga tgcttcagaa actatgctga aaatggcaga
    11101 agaaaagatc caagcagcag atctgaaaaa tgtccgaacg atccacgctg atgcttctgt
    11161 cgaatttcct gctgtccaag ctaatctcat ccttctatca cttgtacttt tacacattcc
    11221 tgatacggag aatattctta caaaacttta tgaaatttta gctcctggcg gtcaattgat
    11281 catcgtagac tttgacaaga atgaacaaat tagtcatccg aaagtccata atggctttag
    11341 ccaagaagaa ttgaataatc gattggaaaa aacgggcttt gtctctgcta ctagtcatac
    11401 cttccatcgt ggggaaaagc tatttatgaa caaaaacgct tcgcttttct tatccatcag
    11461 ccaaaaagct taaaatgaga ttgcaaaaaa agtgagcaac tccaagaaat aagaaggaat
    11521 tttcggaaaa agaagctgtg acaaaaaaca tgtcacagct tctttttatt ttttcatcac
    11581 atgattttaa tttctgaaag aggccaataa cggaaaacaa cttttccttg gatcatatct
    11641 ttgtcgacta acccaaacat tcggctgtct tttgaaatca agcggttatc gcctagaaca
    11701 agatactttc cttttggcac agtatattca aagtcactcg taaaggtgga tgaactttct
    11761 gcttgtgctt ggaatcctga gctataagca tattctcctt gtaacttatc ttctgaaaat
    11821 tcttttttga actcatccag ataaggttca tcatactttt tgccattaat ggtcaactgg
    11881 tcattttcca tcttgactgt atctccaggc ataccgatga tccgtttaac aatcatccgt
    11941 tcttcatccc ccggttcctt tgtagtaata atatccattc gttcatagtt tgagatctta
    12001 gatgaaatca atcgttgtcc atcacttaat gtagggtcca tcgaatgccc gtttacagta
    12061 actggtgtaa acacgtacac tctagctaat aaaatagccg ctaatacaag gatcaaaatc
    12121 ccccaatttt tcaaaaattc tttcacagct tacctcctac ttgtattttc ctcgttattt
    12181 attacgtttt ttcacaatac ttcgttgtac tttctttatt ctacaatgtt tataaatgaa
    12241 aggaaagaat aagtgatgtg ttcgtcttat ctttctcgtt atataaaaaa ggaacgtgac
    12301 aaaagacgtg tcacatcccc cttattccga ataaacgttt ttatcgcaac cactgataca
    12361 cggcgtttca tcacattatg aatgtcttct tctttggcta cgactataaa ccactggttc
    12421 ttcctgcgac tgtgcctttt ttgcaatcaa tttcagacga taaggctcat gatagcgtaa
    12481 cccgtctttg acaaaatcac cgccgacgat gaacaatccg acaggttcca atgtgtcttc
    12541 tgttgagtca agtcgaatgg tccctacttt ttccatgtcc gagtagcttc gtccaatatt
    12601 tagaacatac tctttctctc ctgttttctt gacatagtaa taaggataca atgtatttct
    12661 atcgaccaga aatccatcca tgcgaccgag ttcatttgtt ttgatctcac catttccaat
    12721 caaataccgc atattctcgg ctaatttcaa ttgataatct tcgtcataag aaaccatgac
    12781 ctcttgtgta ttcactttat cgatcgaatt tccattatct atcgattctt ctgtctggat
    12841 gttgaacgct tcatcaggta aatacttcac aaaaagctga gtgacatcgg ctttcgtgtt
    12901 tttatctaaa aagtaataaa atacatttaa aacagtaaaa taaacaagca ctagaaatgc
    12961 aagtccatat aagaaaaatt gaagatagtt ttggttttgc caaagtccat aaacaattct
    13021 caataaataa agcgtgggaa tcacacttaa aatcgtatat ccacggttaa gatatttagg
    13081 actgatgtct aaatagccaa ggaatttatg caccccgttg ataaggtcta gtaaaaatgt
    13141 cattgagaat cacctgcttg ctggttatac gtttgggtat ttcctgaatc ttggttttgc
    13201 tgtgctgtac gtgctgcatc atcattttgc gtgttatcat ctacagtcgt actgcttggt
    13261 atcgtttcgg agctagcact tgtatccgga cttgtacttg gattggttga atccgatgga
    13321 gtgccatctg gtaccgtatt acttgtttcg cttgagctgt cagttgtata tgtctcatca
    13381 tacgacgaac tagttgttgt atcgtcccat gtacttgtcg tagtggtttc attcaaactt
    13441 tccgttgttg ttggttcaga tgtcgagact gaagtagttg gtgttgtttc ttctacagta
    13501 ctagaacttg aacttggtgc acggtagctg gaactattca caatacctgt agtagtcgcg
    13561 acaatcgtcg agattgttaa tacagcgatt ggcttcaaaa taggtgctac ttttcgcatc
    13621 ttatctcttc cttcattgaa atttattctt gtttccttac tttactacgg acaaattata
    13681 cagtctacct tatttctacc ataaattctt tatttttttc ttacgttttt ccgaaagatt
    13741 tctttaattt tcctcaaaaa ctacggttga tgtttttaag taagcttttg cttcccgatt
    13801 gatttctttc agatcgcatc cttgctgctt ggcagcgaac acgcaaccac aataacattg
    13861 gcgataaata tcatactctt tgcacatttg caaagagcgt tcatagcctt tgtttttctt
    13921 gaaatcactt ggcaagtatt gtgtcccgta aatcttttgg atatccattc cgatctgatt
    13981 gatcaaagct gcatttttct ttggcgagat ggtcagggca cttccaaagt aatcgtatcc
    14041 tagttcttgc gctttttgcg caacaagatc taatcgcata ttgaagcatg cactgcagcg
    14101 agcaccacct tctttttcgt tttcaagatg gtttgttaaa accattttgg tgaattcatt
    14161 tggacggtat tcatcaacga ttagcgcaac atgatggtca gttttctcat taaattggtc
    14221 tacaaatcgt ttttgttcat gtaatcttcg caaatactca cttttaggat gaatattcga
    14281 atttgaaaaa aataacgtga tctctgcata ttgagacatg aattccaatg tgtgcgtact
    14341 gcaaggagca cagcatgtat gtattaatat cttaggcttt acttcttctt tctcccattg
    14401 catgatcatt tttttcagaa ctgtatcata atttattttt tgatttttca ttttttctac
    14461 gatttgttga gcattaatca atttgttatt cctcctcctt cttttttcta ggattttcct
    14521 tattataagc catttctatt ttaatcgaaa gctgtattgt tatctgtttg ataatttctt
    14581 tgagtattcc taattcccct tgtatactgg tagaaacgac tattgtcagg aggatagtac
    14641 tgtgcatgtt gatttattct ttcgaaggct gcagcggcga ggacagaagt ttgctgccaa
    14701 tcatttctca tcgatccaaa tcatcgtttt ttattatata ttgatgacgg tcttatcact
    14761 cgttctattt tatatgccga tttttcgcga accagatagc catgtttctt ttgtggacat
    14821 gctgttcatg gcaatcagta cagtgagtgt gactggacta tcgacttttg atatcaattc
    14881 tgtgttcaat gataacggta ttatactgct ggaaatatta tttcaagttg gtggtttggg
    14941 gatcatgatg atttctactg cttttgtcat tttttctaaa cgacggatca cactgaaaca
    15001 gcgacagctg atcatgacgg atatgaatca accgcgtttg tctggtatcg tccgactgat
    15061 tcggatcact tttgctattt taatctggtt tcaattactt tttgggacat tcttttctat
    15121 ttatttttat tacagaggct attttgatcg ttggcgtgat gccgtttttt acggttttta
    15181 tcaggcgata tcggctgtca cgaactctgg gtttgacgtc actggggatt ccatcaagcc
    15241 ctttgcacat gactattttt tcttgatcct gatcatgttt ttgatttttg tcggaggaat
    15301 tggttttcct gtattgatgg agtgccggga atggctatta tacaaacgtt ccaatgcgaa
    15361 gcttccgttt cgtttttcct tgtttaccaa attagctgtg ttagcttttg ttatcttgtt
    15421 tgtttcggga actgtcttga tttatctatt ggaaaaggac catttattcc aagattccaa
    15481 tatgagtgtc aaatggatca attcgatgtt ttattcgatt acgactcgta atgctggtct
    15541 gcagattcac gatttgggtg attttcaaat cacaacactg atcatttttt ctttactgat
    15601 gtttatcgga tgcagtccta gttctgtagg cggcgggatt cgaacaacta ctgtagcaat
    15661 catcggtttg tatctatatt ccttcctcaa aagtgaagat aacatcaata tttttggacg
    15721 gagaatcgat caagatgatg tgcgaaaatc agtcgttgtc tttatgcttt ctcttggtat
    15781 gtgtttcttt tgtatcgttt ttttatctgc tacagaagaa caaacgctga tctcgatcat
    15841 catcgaagta acttctgcgt ttggaacaac tggtttatct ttagggatta ccggagactt
    15901 gtctgttgtg ggcaaaatca cgattgctac actgatgttt attgggcgga tcggaatgct
    15961 atatacattg atgttattcg tgccaaaaga aacacgagat ctaggctatg aatatccaag
    16021 cgagaaaatt attatcggtt aaaatgggaa tataggtaag agggaataat aaaatcacag
    16081 cagcttgaaa caatcatttt gtttcaagct gtttttttgt taaatcatgc gattttgtcc
    16141 gatttcatgc actttcagcc gtaacctttt cttaagaata agaaaagcaa tttaaccttt
    16201 ttttcaacat atggtatgat tagttgaatt tattcgtcag caaatcgttt ttggaggtta
    16261 cataatggga atacgcagtc attttgcgat tgcagttgga aaaacgtcgc aatgggcatt
    16321 aaaaactttt tttaaaggcg gatcaagttt accaggtaaa ctagcgctga aagtcgatcc
    16381 acatatacta gatagtttgg caaaagacta ccaagtcgtc gttgtcaccg gaacaaacgg
    16441 taaaacattg actaccgctt taacagtcaa tattttgcgc cagcaattcg atgaagttct
    16501 aaccaaccct actggtgcga atatggtaca gggaattgta tcgactttct tgcaggcaaa
    16561 accaaaaaaa ggacagaaaa agtttgctgt cctagaaata gatgaagcaa gtttgagcaa
    16621 agtcacagaa tatatgaaac cagaactgtt cttatttaca aatattttcc gtgatcaaat
    16681 ggatcggtat ggagaaatct atacgaccta taaaatgatt gtagacggtg ccgcaaagtc
    16741 accaaatgca acgattatca gtaacggtga ttcaccaatc ttcaattcta tcgaaacagt
    16801 caatcctaga aaatattatg gctttgacca tgaagaagat cgtgaacaaa tggctcatta
    16861 caatacagac ggtgtcttat gtccaaattg tcatcacatc ttgcattata aaatgatcac
    16921 ttatgcaaac ttagggaaat attactgtcc aaattgtgac ttcaaacgtc ctgaattgga
    16981 ttatcgatta actgatatgg tacggatgga caatgtgtca gcagatttcg tgatcgatgg
    17041 tcatgaatac ggaatcgaag ttggcggtat gtataatgtc tacaatgctt tagcagctac
    17101 tgcagtggca gagtacttca atgtcgatcc tgataaaatc aaacaaggtt taggatatga
    17161 cgagaaagta ttcggccgac aagaagtcat caatatcaat gggaaaaaat gtacattagt
    17221 acttgtcaaa aatcctgtag gtatcaatca agtaatcgat atgattggtc tagctcctta
    17281 tccattttct ttagtggctt tattaaatgc caattacgca gacggaatcg atgtcagctg
    17341 gatttgggat ggtaattttg aaaactttgc cgatatggat atcccagctg ttatttctgg
    17401 aggagaccgt catacagata tgactttacg tctaaaagtt gccgggattc cagaaaataa
    17461 attgacagaa acaaaagaat taccagaagt aatcgaaaaa atcaaagaat tgccaactga
    17521 gcatgtctat attttagcta cttacactgc tgtattgcag cttcgtaaag aactagctgc
    17581 tcaaggattt atcaaaggag gaatgaaccg tggctaatta tgaactcaat atcgcccacc
    17641 tttatggaaa tttgatgaac acatatggtg ataacggaaa ccttttgatg ttgaaatata
    17701 tagcagaaaa aatgggggtt tcttgccata cggagatcgt tagtatacat gaaccttttg
    17761 atgctgataa atatgacttg gttttctttg gcggtggtca agactttgag caattaatca
    17821 tttcaaaaga catccagaaa aagaaagatt ctttgatcaa ctatatcgaa aatgatggcg
    17881 tcatgttagc catttgtggt ggctaccaat tgctaggtca ctactacatg ggagctaatg
    17941 gagaaaagat ccaaggaatc agtgcactgg atcactacac gttaagtcag gaaaataacc
    18001 ggtttatcgg tgatatcgtt atccataacg aagaattcaa tgaaacttat tatggatttg
    18061 aaaaccacaa tggtcggact ttcttaggtg aaggtgaacg acctttgggg aaagtggtac
    18121 aaggtcaagg aaataatggt gaagaccaat ctgaaggtgt catttaccga aatgtcttcg
    18181 gttcttattt ccatggacct atccttgcaa gaaatgagaa cctggcagct cgtttgatcc
    18241 gcctagcttt agaacaacgt tacggtaaag aattggatct gccctatctt gaaggacaaa
    18301 cagcctagtt attcgaaaaa ggggaatgtg acaaaaattt cgtcacattc ccctttttcg
    18361 tatcctattt taagcgactt gcttggcata acggttcacg ttttgcttga tattcccgat
    18421 tgttttcgga tcattgattc ctgtgacttc ttgaatcaat tgttccaagt tttcatggat
    18481 cttcatttca tgcaattgac ggctttgttc atcttcagga tcatagtaat taaaaataat
    18541 ccccattgtt gccgtcaaat gtgggcaagt caagttatgt tcttgtagtt ctctcactgg
    18601 acgtgtaaat ctttcttgat aacctaactt acgtaatggt gtccgagcga cacgtgtgat
    18661 cgcatcagag atatttttgt tttggaagcg atgaatgatt ttttcaatat acgcatgatg
    18721 ctgctctgca tcaaaccccc atttggcaat cagcaaactt cctgtttcct gcaaaactga
    18781 ttttaattgt gccacaacta acgcatcttg cattgcttca tcaatggtct ggtaacctaa
    18841 caaggcacct gtataagcga cagtcgcatg accagtattg acactaaata gtttgcgttc
    18901 gatatatggt tctagatcat ctacatataa aacccctttt aattggattt ttttattttt
    18961 tctttggcta tcgtcaatga cccattcttt gaaaggttcc acttggacga ataaaggatc
    19021 ttcatggtgt tgtaatggga cgattcgatc gactgctgca tttggaaagc cgatatattt
    19081 gtcagtaaat gatgtttccg gaagatattt atacacttct ttttctagaa atgctgaacc
    19141 gccaatcata ttttcacagg caatgatatc taaagatgtt gtgcttcctt ccttttctct
    19201 ttcttggata ccttttgcaa tcaactcagc aataaaaggt aagatattcg ggccaatggc
    19261 ggtagtaact aaatctgctt cagcgatttc ttggattact ttttctggat ttgtttgatt
    19321 gttgattcct ttgacatttt ttacagtgat tttttctttt gattcatctg ctaattcgat
    19381 ttcgtattct tttctttcat ttaaagcatc aatgatcgtg tgattcacat ctacgaaagt
    19441 aatctcaaat ccattagctg ccaaaacctc tccaataaat cctcgaccga tatttcctgc
    19501 accaaaatgt actgctctca tttttctctc tccttaagca attgctagat tttccgttac
    19561 ttcttctttg ctgagcgcgt ctgctagctg gacaacattg tccatatcac tgcaataaag
    19621 tgcgatttgc tgtacaagtt gcaaatgctc atctccgact cctgcaatac cgaacaaaac
    19681 agtcgcaatc ttttcttctt cttcggtacc aaaatctaca ccatctggta cttgaacgac
    19741 acagatgccg gattttttca catatttttt tgcttcatct gttccatgag gaatcgcgat
    19801 aaaattcccc atataaacag ataacatttt gtctctatct accatggcat cgatatattc
    19861 ctcatcaacg caccctgctt ctacaagcat ctgaccgcaa tagcggatcg cttcttcttt
    19921 ggttttaaaa gatcgattga gctcgatcat gtcgaatggc aaactttgca tgctgttttc
    19981 ctcccttaag ctgtctgttt caaatattgt gcaatcgatt cgtatttttg cggattgacc
    20041 atgctgttga ctgataaatg aatggattga ggtgcagcca tctgtacttt ttccgctaaa
    20101 ctttctttgg ctacgatcag tgaatcaggt tcatctttta aatcttggat attgactttt
    20161 cttaacggga tagttaaatg gttcttgtct aatatatctt tgagcacggt catccccatt
    20221 gttgctgatc cggaacgatc atcgtggaca agtagaatct ctttgatttg tttgatgttt
    20281 gctgaaagaa actcttctgc tggctcattc tcaatttcag aaactgatgt tttctgcttg
    20341 ctgcttaatt cttgaatgat ttcatcatat cgaggtgaat tcaaaaagtt ttctactgag
    20401 acaaaacgcg cgctcggtgc tttttgtttt gctctttctt gtagttcgac ctgagtaacg
    20461 atcaatgtat tcgagtcatc tgtcagttgg ctgattgctt gattggtcac cgtttgaggc
    20521 aagtctgctt cctgtacttt ttttctcaag atcgaagcgc ccatcgcact tgatcccatt
    20581 cctgcatcac aagcaaaaat gattttttgt acgccagaga atgtagctcc ttgaggaata
    20641 ccttgggcac ctttggaagc ttgcttagct gcctttgtct cttgaacaga tttttcgaag
    20701 tcttcatttg tatctttcgc atccgctttc aaaattactg cagaaatacc aaacgaaaca
    20761 gctgcggcaa caatcacgcc caaaataaca ggaaggtaag aattgaacgg tgtcatcgct
    20821 agaatcgcaa tgatcgaccc tggagaagct ggcgctctta gtccagcatc catcaattgg
    20881 aaaatgaaac ttcctgacat tcctccgcct ataactgata agaataataa cggtttcatc
    20941 ataacgtaag gaaaatagat ttcatggatc ccgcctaaaa agtgaatgat catggctcct
    21001 ggtgctgaag attttgctgc tcctttgcca aacaaagtaa aagcaagtaa tacgccaagt
    21061 cctggaccag gatttgcttc aagcaagaat agaatagatt ttccagcctc tgctacttgt
    21121 tcagttccca atggtgtcaa aatcccatga ttgattgcat tattcaaaaa taagattttt
    21181 gctggttcaa taaaaatact tgtcaatgga atcaaatggg cagataaaat gatctctaca
    21241 ccttttgcca ttgcttgagt caatgcatcg acaactggac caatcgcaga aaaacctaat
    21301 aacgctaatc caaaaccgat aagacctgct gaaaaattat tgactagcat ttcaaaaccg
    21361 gatttgatcc gtttttggaa tagctgatca aatttcttga tggcatatcc accaagaggt
    21421 cccatgatca tcgcgcctag catcattgga atatctgttc cgacaatcac acccatcgtt
    21481 gcaattgccc caacaactga tccgcggtca ccagcaatta cttttccgcc agtataaccg
    21541 atcaataaag gaatcagata agttagcatt gggcttacca tggtagcgaa agcctcattt
    21601 ggtaaataac catctgggat aaatagagcc gttaatacac cccaggcaat cagtgctcca
    21661 atatttggca tgaccatgct tgataaagta ctgcctaatt tttgtaccct cgcttttagc
    21721 gaaatgttgt ttttgggaat cgtccgttcc attttgtctc tccctctctc cttttgatac
    21781 tctaattgta tccgcttact aaaaagagaa atagtcgtac tttggcacga aactttgtgc
    21841 caaaggtttg agtatgtcag caaaataaaa agatttcggt atcagaaaac ttcaatttta
    21901 aataaaactg ttattttaat ttttttataa tatgcaaaca ataacttttg ttagcattta
    21961 atttagatgt tttccattct attatgttat taaattttat attggaggcc tatctatgac
    22021 attactgatc aaaaacatga acatgaaacg tggtaaaaaa cgtgtgattc gacaattgaa
    22081 tcttttagta gaatcaggag aagtagctgc tttgattgcc ccgaacggat ttggcaaaac
    22141 aactttgctg aatggcattg cccaactgct gccaacaact tatgatcaat tgtcccttca
    22201 caacatctct cctactgctt atacagatta tcgccggtct ttcttttttc tagaatcaac
    22261 gaatcagtta tccgactatc tatcggctgt tgattatttg actttgatca aatactattg
    22321 gcagagcaaa gtagaatgcc aaactgtttt ccaacgattg aagatgactg agtaccttac
    22381 tttgccgatt ggaaaaatgt ctctaggaat gaaacaacat gtcttgcttg ctgcttactt
    22441 gatcagtgat tcatctgtca tgttattcga tgaaccgttg aacggattag acccaggaag
    22501 tattgatatc ttgaatagcc tcatcagaca atggcagaaa gaggggaaga cgattcttct
    22561 ttcttcccac gctatcgcga atatccaatc gatttgttct aaagcatgtt ttctaaaaaa
    22621 tggagaaatc gtcacagaat cctcaaatat ggaagagatt ttagctattt atcgtaaatt
    22681 atatgaggtg taactatgtc tttacttaca tttgaacgca atctcttact aaaaaacaaa
    22741 gtaaactttt tatttcctct attgttggtc gtgctctttg cattcccact tttttttgat
    22801 cataaacttg cttatacaga attcgatgaa ctgaatcata actatgaaga gatgcaacgt
    22861 ttgattgaaa cgttgaaaga agatgagaat gagaaagaat ttgtggaaag tttagagaaa
    22921 agcaacaaac tgatcgaagc aatcttacat gcaaaaaaca cagggaatgt ccagcaaatg
    22981 gtcgaagcaa cttatcatta tgaaaaagat atactggatc gactgatttc tggtcaaaga
    23041 caaggaattc caatcattga acaacaaaaa cgtgtcgagt tgctccgtta tatgaaagaa
    23101 catcagatcc aaagatattc tatttttgac ttgccagcgc atctctcttt agcaaattac
    23161 tacgaaaata ttttttcagg aatgatttct agttttttga ttttatgtat aaccgccttg
    23221 tttctttctt ccatcatctc ttatgaaaaa agaaagcagg taatctctct agtcaatcta
    23281 ttactggata gtatggtaaa gaaacatagc atccgtttta ccatttatta tggtgcagct
    23341 atgctaagtc tcgtgatgcc atttttgatc gtctctatac tagtaataat caaaaatggt
    23401 ttgggagact tccgttatcc tgtaggaacg atcattggtc aggagatacg aatcctgccg
    23461 atgtatgagt atcttttcca gagctttctg ttcctgttgt tgtgggtgct ctttttaagt
    23521 acaatcagtt ttcttctatc tgctttattt gaacattctc tagtcaatct tttaggtaca
    23581 cttctttgtc ttttcttagc agagtatcgc ttgttttctt cgattggatg gatagaatca
    23641 atcagtcatt acttgccaac ttcttatgtt gattttcaaa atgtgatcat aggaggagat
    23701 attttctcgc cactagcttc agaacaggtt actttcatga atggtatctt gacacttggt
    23761 atatggagta tcgttttatt attcataggt atgggcacta tctatataaa aaagagctat
    23821 taaaacacaa aaataatagg ggcggtgaca aaaaccttgt caccgtcccc tatttcaaat
    23881 aaacggtttt ctagaataaa tagttttcaa aagcttgcat ccgcagtaat aagaacctcc
    23941 caacaaaaat agctcctctt atttttatct tggttgaagt tattacttga tgcaaggcag
    24001 ctttttttac tgtgaacatt ggttatatat gtccatgttc aaaaatcatt tggacttctt
    24061 caatggtttc cgcatcactg agcgccatta cttggtcgat gtctgaacag aaaaaggcta
    24121 aatcttggag caccgctaac tgatgttctt tttttaaagc cactacaaat aagagtgtcg
    24181 cgatttttgg ttcctctttt gttccaaagt ccacaccatc aggcacttgg actaaataga
    24241 ttccttcttc tactacttct tcatctttgt cagtcccatg tggcaaggct acgaaattcc
    24301 cgatatagac actcgctttt tcttgtcttt cgatcattga ttggatatat gccggtttga
    24361 ttcccccttg ttgggtcatc cactggccga tctcggtcaa cgcttctttc caattttgat
    24421 aggattgatc gagcaatatt ttttctgctg gtagctgtat catcttttca ccttcatttc
    24481 tttatttctt caatcaggat tttcgctaac aactgataaa cgatttcttt atttcctgag
    24541 cggaagatct cggtgttcaa gtcattcatg atgattgctc cactgatctt tcctaaaaga
    24601 atcccgtctt tttcttggat tggcgaaggc gccagcataa ctaacatacg ggacaaatcg
    24661 actggctgtc tatccatccc ttcaatggtc aacggatgtt tcaaattgaa aatacaaaag
    24721 atcggtttag aaatcaatga gctggatgta tggaataacc ccatatttgt atgaggaata
    24781 ccgataggtg cttgttcata tcgcttaagc agccggcgat gaaccacctc ttcatccgtt
    24841 actagtcctt caggaaaata aggaaataag ctgcgaatcg tttcggaaat cgtctcagga
    24901 ttatccagtt cttggataaa aaaacgaccg agtaattgat tgatttgatc catgaaatgc
    24961 attgtttcct cataagtatc ttctatcatc cactcgttat tgctgttata cgtaattttt
    25021 tgcctttttt tcttctcaat atgcgaaaaa gcttctttta gctgcttgac ttcatcttct
    25081 agcagtagcg gggagactaa aatatatttt ccaccgtatc ctggtaaaat agatgtagaa
    25141 ataacaacat catattcttc ttgtaaatcg atttttttta aatcagcaat ccgatagaaa
    25201 tgaatttgtt ggatgaaggg aaaatacctt ttcaatcgta gttccaacat gctagtagaa
    25261 gctaatccac ttggcgaaaa tcctgcaata ctgatattgc tttctttcgg acttcgttcc
    25321 aatgaattcg caaaatgaag gaccatataa gcgatctctt cttcggataa ttttttttct
    25381 tgaaaaatcc gctctactgc tttacggatt gcattcgcaa cttcctcgta ttgaaccatg
    25441 attttttcta aaatcggatt ggtcagtgtc tcttcttgta aaacggctct tgaaaacaca
    25501 ccagataaat gagtcaacag cattttgtac aaagtttcat cttcataaaa atggacattc
    25561 gtctcttcac ttacttcttg aatcagttgt tttactaaat aagcaagctg tgtatcgaac
    25621 gtctcttcaa agaaattctg atcaaatgag ttcgtgaagt catttaataa tcctgcaaaa
    25681 aagacgatct catttactgg ataaagttgt tttgtctcag aagcgatccg tgaaaacaat
    25741 cgtttagctg tctgtagtaa ttctttgttt gccagactat tatatgtctc atttgtcagt
    25801 aaatgatttt tcgtcacacg ctccatcgtc aatgtcagca tcaaaaccaa atgttcaagc
    25861 tttctatcac ttagattggt ctttttttgg atcaattctt ccccgattac tttgcgtgca
    25921 agagatagat gttgcggatc aacgaatcga aaaaaataat tcagtgatgc atcgaaagaa
    25981 atactatgaa aaagttcata ttcattgatt tctaattcta atacattcgc tgtcagaagc
    26041 cggcggtatt tttccggtcc ttcgatctcg tatccctggt tccgaatgat ttccaaaggc
    26101 agttggcgaa tactttcttc caactgtttg atatctgcat aaaaagtggt attactgatg
    26161 cgatatgatt ccatgaaatg agcaagactc aacggctctg tcgtcagtaa caactcggct
    26221 aaaatcgcgt gttgtcgttt agcagtggat aagtcattac ttttcccatc tgtattttct
    26281 tttaattgct ctaatacttc ttctgatgcc tcaatgacaa atgagcctct agctgctttt
    26341 ttcaaagaag catttaccga tttcaatgag acttcaagat tatctaattc gcggtaaata
    26401 gtccgtttac tgaccttcaa taaattcatc attttttgga tagatacagg tgacgggcta
    26461 ctgagaaatt ctgctaataa ttgtttttct ctgggcgata aatacatctt atatttctcc
    26521 cttcacgagt ttttcgtatt cattcgtagc tacccaatgt tcaactagaa ctagccgagg
    26581 gatcttttct tctagctgtt cagttacagc tagttctctt tgtgcgatat aaatcgtttc
    26641 ttcttggatt ttgatttttt ctattgattc ttttgagata acaaactgtt ttttcgcttt
    26701 ttttgctaat tgacttaaaa tcgtcatgcc catagtttgt gagccgcgtc tgttgttttt
    26761 atacaaaaat acaatattag caggcgacgt tttttgttct gtgatatctg ctatctcttt
    26821 tttttcttcc cgctttggaa attggatcgc ttcttttatt cgtgacagca agatatctgt
    26881 ttccaaaaag tgatccagta catagatttg actttgaggt actttttctc ttgcaagacc
    26941 cgctagttca tgttgcacga taaccagctg atttacttgg ttattcaatt catagataga
    27001 gctgtatccg acagaaagat cgattttttc tgctgctaat ttctttttca gcagggcggc
    27061 tcccatagca cttgatccca tacctgagtc gcaagcaaaa atgacggaat gaatgataga
    27121 tggttccaag atttctcccc tttctttgac cgttttgttc tctttttctt tatttggtaa
    27181 tttcaatagt atcgctgcac aaatgaaggt cacaaatgta ctgattgcta tccctatact
    27241 taccccagct agttgtggat aaggcgcatt ggcaaaaatc acgagtagtg acccaggcga
    27301 tacaggtgca gctaatcccg tatctaacca ttgaaatatc aacgtgccgc ttgccccacc
    27361 agccattaca gctaaaaata gatacggtcg cagcaagaca aatggaaaat agatttcgtg
    27421 aatcccgcca atcgcatgga tcatcaatgc tccactcgca tttacttttt cctgtttttc
    27481 tgcaaaacaa aggatagaaa gtaagatccc taaaccaggt ccaggatttg cctccatcaa
    27541 aaataaggaa gagtacccga ctgtttgtga gaattcgatt cctaaaggtg tcaatacacc
    27601 atgattgatt gcattattga aaaatagtac ttttagaggt tctaagatca gactgacaag
    27661 tggtaacaga ttcattgtga ctagccaaga cacaacggag gcgagcaaca agtgaacact
    27721 atttaaaact ggttcaataa aaacgattcc aattatacaa ataacgctac cagtcagtcc
    27781 tactagtagg ttgctgacaa gcatttcata tccagccttg aatttcacat ggaatctaga
    27841 aacaaattgt tcatatacga tgccggcaag cggcccgata atcatcgctc ctacgatttg
    27901 tggagcattg gaacgagcga tcaaccctaa agaagcaatt gcaccggcga ctgccccttt
    27961 ttgtccccct accgatttcc cgcctgaata actgatcaag atgggtaaaa gcagctggat
    28021 catccaatgg catacctctt ctaatgtggt gtgcaaatcg ccagacacac cttttgttgc
    28081 taatgacaag attccccaag cgataaacag acttagattt ggcatgatca tttcatttaa
    28141 gtgatgtcca gcttttctca gttgtttcat agacacgcga ctgctgttgt ttgctttcca
    28201 tttgtattcc atctttttca ccagacttct atttattgat ggacggattt tatgtagaaa
    28261 aagcagcaaa cgaaaaaccg tctgctgctt tcagctatct gggaactatt tgcctggttc
    28321 agacgcaatg attgctagtt caccttggac cgcccactca gaaacgccat ttggaataat
    28381 acagcttgtt cctttttcta atggatatgt ttttccgtca acgacaaggt ctcctgctcc
    28441 ttcgattacc gtcatcaatg tataaggggc ttgttttttg aaagatgtga ttcctttaat
    28501 atcccactca tatacattga aaaactcagt ttctaaataa gtgacgatcg atgaattccc
    28561 ttttcggact tctttgattt gtaattctgg tgttttcgca gggacagttg ttacatcgat
    28621 cgattgttgg atatgcagct ctcttgtttg tccttgcgca tcttttctgt cgtagtcata
    28681 cacgcggtaa gtcgtgtcag aactttgctg tgtttctaaa atcatgattc ctttaccaat
    28741 agcatggatc gttccgcttg gtacataaaa gaaatcacct tttttaactg gtactttctt
    28801 caaaaggtca tcccagcgcc catctttgat catctcagct agttcttctc ttgtctttgc
    28861 atgatgacca tagatgattt ctgcaccagg ttcagcatcg atgatatacc agcattcagt
    28921 ttttcctaat tcaccttcat gtttcatccc ataggcatca tctggatgta cttgtacaga
    28981 tagatcatct tctgcgtcta agatcttgat caataatgga aacactggtt ctgaaggatg
    29041 gccaaacaat tccttgtgtt ctgaccatag ttcatctaat cttttccctt tgaattcgcc
    29101 attttctact acactgacac catgaggatg cgcactgatc gcccaatctt ctccgatttt
    29161 atcgttaggg atatcaaaac caaagactga acgtaaacga cttcctcccc aaattttttc
    29221 ttgaaaaact ggttttaaaa acataggttc ctgcataatt caaactcctc tcatttttca
    29281 tagctattat agcattattt cggtaatacc gctttctctt tcaaaaagtt ttcaacaatt
    29341 tcatctcgac atatgagata ctccgcacga tgatcgacag gatgtacgcg atttgacaaa
    29401 aatatcaacg cttcttgtgt ttttatatca atcaagaaaa acgtacctgt ataaccagta
    29461 tgaaaaagaa tcgcatgacc gtcttctaca tcctttttta agtcccatcc taaagaacga
    29521 tagccatttt ttttcggtgt ttgatcttgc agtaaagaat caatcgtctc ttcttctaaa
    29581 aaacgaccat tacggatttt tcccttgttc aaatacattt ggacaaaagt aatgctgtct
    29641 ttcaaatcaa taaagagacc cgcgttacct gcatgttcag ccaaaactct tgctttcgga
    29701 tcatgagttt gtccacgcaa aacctcaccc gaaggcagtt gttctgtggg aacgattttt
    29761 ttggaaggaa aaggaagaaa taagctgttc atcattccta atggttccaa tacttgttct
    29821 tgaaagattt ccactgcagt ttcttggtaa atttcctcta gcatcaaccc aagaagaatc
    29881 gtaccggcat ctgtatattt tacttctttt cctagttgct cacctggttc tagcttcaaa
    29941 taagcggcga tcaattcttc ctgagatagc ttatctctgt ttggtatcca tgttttgata
    30001 tcagaagtat gtgtcagtaa atgacgcaaa gtgattgtct gattttcaaa ttcaggaaga
    30061 tatttatgaa gcggttgatc gagttctatc tttccatctt ctaatagacg taaaactaat
    30121 gaagtggttg caacgacttt cgtcagtgag gctgcatcaa acaacagatc ttctgtcatc
    30181 agctgctttt caggaacgac ttgggcaaag ccggctgtaa atgtttctac ttgattttct
    30241 tttatgaagg cgactgctac acctggataa aatccttctt ccatcttttt caaaataagc
    30301 tgtttcgtgt gtggatacat ctttttctcc tgcttttcta tcagattctt acagtccaag
    30361 cttaaagctt aatctatgag gaatcaacct tttttacaca aaaaaactgc gcgaaaaacc
    30421 gcgcagtttc ccccggtata ctattttctc tttctatctc tagaataagc gatagtatga
    30481 ccgttcataa aatgacgaga catgcgacaa tcgttaagaa agaaaaagtt aagcgtcgca
    30541 tcgaatgtca gttttattct tttcttttgg ctcactgacg aaccaccatt cgattcctat
    30601 agcatcatag attgtgagta ctgtgtgctt tttgtctaag aagaaatcaa ctgatagttg
    30661 ctgcaagtga gcaatcagtc cttccatgct ttcagtatcg atcatgaatt tcagaaaatc
    30721 aagtccaatc acttcatctg taggcaaatc aattgcgcct ccgctagctt ctgtcaaacc
    30781 aacatggaaa tctccttcat tcaatacatg gatgccctct tcttcattat gtaccttcaa
    30841 accaagtaca tgattaagaa attggcgttc ttttgttaag ttagtaacat ttaaatggat
    30901 tttttcaaaa taagaacctg ttgatagttt actgaatttt tcttcagatt ttcttaataa
    30961 tgcttcttga tccattgttt ctggaatttc tctttggtcg ttcttattct cttcatggta
    31021 aatttccaat tcattctctt ctgggtctac gagcaaaatc cccatacgtc catgatcttc
    31081 taaagcttct ttgattggat aattcatttt acggatacga caaaggatat ctgccatttc
    31141 ttctgtgcta gggataatca atgaaaaacg gttcagcttt ttgatcaccc ccatgtgatc
    31201 attcgctcta ggactttctt ccagcagcaa catttcactt ccagcttctt ctgttcctaa
    31261 aatggctaat tcgttttctt ctcttttcaa atcaaagcca acaacgttct tgtaaaaatt
    31321 aatcatttta tctcggtctt tcacccgaat ggctacactt tttaaatagg tggcagcccc
    31381 caattgaaaa tctttcatat ttcttgctcc cctctacgtc aagctttatt ttaacgatgg
    31441 attcttcttc tttcaagcaa taactttgat tttgttaaaa aaaatgtgag aaaattcgca
    31501 ttttgcttgt tttttgtaaa ttaatgagta aaaactctta taaaaagagg ttgtgaaaaa
    31561 gctgttctgc atcaagtaat aagaacagcc aaacaaaaat agctcctcct atttttcgtt
    31621 tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    31681 aaaagtcgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt ctacattttt
    31741 gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttacc aagaggttgt
    31801 gagcctgccg tttaactccg agtcacaagg agcaatttca aaaaacagct tctcaggttt
    31861 gttgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta aggaagaggt
    31921 tgtaaaatca gcggtttgac ctgagtatta gagatacaaa acggaaaaat agcttctcat
    31981 attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccgt ttattcggaa
    32041 aaagggactg tgacacgact tttgtcacag cccctaaaaa aaataataaa tttgtactat
    32101 tctatgaagc ttcttcttca gaaaactgac tgttataaag atctgcatag aatccgtctt
    32161 tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga accatgattc atcacaataa
    32221 tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac aacgaaactt gtccggtttt
    32281 ccaataggcg gttcatcgct ttttgaatca gtatttctgt tcgtgtatct acactagatg
    32341 ttgcttcatc aaggatcagg acatctggat ttgctaagaa tgcccgcgca attgtgatta
    32401 gctgacgctg cccttgcgaa atattgcttg catcctcgtt taatacagtg ttatatcctt
    32461 ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt agcagcctca atgatttgtt
    32521 cgtctgttgc atcgtcgttc ccatatttga tattgtcata gatacttcct gtaaacagcc
    32581 aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc atcacgtgaa agatcacggg
    32641 tatctacacc atcgtaacga atactacctc cagaaacatc gtagaaccgt tcaagaaggt
    32701 tgatcagtgt cgtcttccct gcaccagtcg gacctacaat agcgaccatc tcaccaggtt
    32761 tgacatccaa gttaaaatca gtcattagca agttatctgg tgtgtagccg aattgaacat
    32821 tttcaaaacg aactttgtat ggtgtatctt tttcttctgg aaggccagat ttttcattgg
    32881 acatctcttc ttcatccaac acttcaaata cccgttcggc tgaagcaact gttgattgga
    32941 tagtgttcat caaacttgca agttgagtga ttggttgaga gaactgtgtt gtatattgca
    33001 agaacgcttg aacatcccct aatgtcatgt tcccatttgc aacttttact ccgcctagta
    33061 cagcgacgaa cacatagcct agatttttaa cgaaattcat caacggcatg atgattgccg
    33121 aaataaattg agctttccaa ccagcggcat acagattctg attttctttt tcaaatactt
    33181 cctggtcttt ttcagtatga ttgaatgttt taacaatggt atgaccacca tatgtttctt
    33241 ctacttggtt attcaataaa cctaaacttt tttgctgatc tgcgaaaaac acttgtgatt
    33301 tcggcgctac gatcatcacg atgatcaagc ttaaagggat catcaataac gcgactaacg
    33361 tcaattgcca gctgattgaa agcatcatca cgatgacccc aatcaatgtg actgcactag
    33421 ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat gttatccata tcattgattg
    33481 ctcgagacat gatatcccca tttgtatgtg tatcataata ggaaatcggt acacgattca
    33541 ttttggcttc caaatcatga cgtaattcat aaactgttcg ttgggaaaca cgtgtcataa
    33601 taaattgctg taaaaagttg aatacagcag aaatcaaata catcacgatt actgtcgcaa
    33661 tgatctgcgc gattttatca aagtctatcg gaaattttgt tacttgttgg ccttgttgca
    33721 tctgttgagc gccagccatt acacctttga atatttctgt ggttgctttc cccaagacct
    33781 ttggtgtttg gatttggaag atcacggctg ctattgctag tacaaataca gcgatgatcg
    33841 caaccatacg tttagacata tagccaaaca atcggcgtac cgttccccag aaatttttag
    33901 gcttttcacc ttttacaccg agatttctgc caggtccacc atgtcctgga ccgcctgctg
    33961 gtccatgaga gggacgtgtg tttgttttac tcatgctaag tcctcctctc ttagttgtga
    34021 atgaacgatt tcttgataag tttcgtttgt tgctaatagt tcttcatgtg ttcctttgcc
    34081 aaccagtttt ccttcatcta gcacaaggat catatctgca tccattactg tacttactcg
    34141 ttgagcaact aaaacggtga tactttcttt catattcgtt ttcaatgctt ggcgaagatt
    34201 ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca aacacataga tatctgctgt
    34261 tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt ccaccagaga agtttcctcc
    34321 tccttgttcc acatggctat ctaagccatc ttccatctct gaaacaaaat ctttagcttg
    34381 tgcgatttct aatgctttcc aaatcatttc atctgttgca tccggcttac catactgcat
    34441 attgtctcgg atcgttccag agaacagaac tgctttttga ggaacaaagc ccattaattc
    34501 tcgcaaatta tattgagtca tgtcactaat gtccgttcca tttatttgga tcgaaccaga
    34561 ttcgatgtca tataatcgag ggatcaaatt gactaaggtc gttttacctg aaccagtccc
    34621 ccctatgatt gcgacgatct cgcccgactt ggcttggaaa tcaatatctt ccaatgcaag
    34681 tttttctgct ccatgatatc gataattgac atgattgaag gccaatgttg cgttttttcc
    34741 agcaaaagaa actgttttag gattttcagg gtcatggata ccgatttttt catccaacac
    34801 ttcattaata cggtcagctg aagcttgtgc acgaggcacc atgataaaaa tagcggaaag
    34861 catcataaag ctgatcaaga tctgcatcgc ataagtcata aatgcaatca gattaccgac
    34921 ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa gtgattgcta cgtttgtacc
    34981 gctcatgatc aatgtcatca ttggcatcat caatgcgacg atcgtattaa ctttgattgc
    35041 cgtatttgta taatctttat tcgctaaatc aaaacgattt tcttcaaagc gtgtcttatc
    35101 gaatgctcga atcactctga cccctgtcaa tccttctcga aataccaggt ttaaacggtc
    35161 agttttcttt tgcatacttt tgaataatgg aacagcaaag tacatgattc cgccgatcaa
    35221 aataatcatt actgggatga caactaagaa gatttttgtc aactgatgat ctttttgata
    35281 agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg atcatcatac gtaaaaacat
    35341 ttgtgtaacc atttggattt gagtcacatc attggtcgtt cttgtgatca aagaggccgt
    35401 tccatattta tcaaacgcat cttttgtcag actctctgat tttttataga catccgtacg
    35461 caattgctta cccagtttct gtgattcacg agtagcaaaa aatgtatttg cgatagcagc
    35521 taagatactg atcaaagaaa agccgatcat cacaaaacct gtatgccaaa tataatcgac
    35581 atctccttgc gctacccctt tatcaataat attggaggta agtgtcggca aatataaatc
    35641 tgccagtact tggatcacca tgaaaactgc agctgcaatt gctgaagtca aggagatccg
    35701 ttttacgatt tttagcatct agttcccttc tttctattta tatactccgt ttttcagcca
    35761 agaaagtttc aaattgaatt cactgattgc ttgctctact gagtaatctt cagatgttga
    35821 aaataagcga tacgaatgcg caattgttgt aaatacagta ttcatcacta attggatcag
    35881 taattccaat tcatgatcat tttttacttt cagcttcgtc cgatcgatca attcaagcaa
    35941 ctcttttgca tcttgtttgt gttctttatg tggatcgtga ggcgtttttt ggcgactcgt
    36001 atgaggagcg acttttcttg aggcacggta atccatgttc ataaacaagt tacgataaaa
    36061 agaagcattc tcgccttcca aaatctcata aatcatttta gaaaaatagg attcgaatcc
    36121 tttgaataga tcaccatcgg attctttcat tagttgtaac atatctttag agctgtcacg
    36181 tctcattgtc tcaaagtaat aaaaataaag atcctcttta tcttcaaaat actgatagaa
    36241 actgcctcta gggatatccg ctaatttgac gatattagca atcgaagctt cttttaaggg
    36301 tactctagaa aactcgattc tcgcagcctc gattagtctt ttttgttttt ctttaggcaa
    36361 atggaaaaat gtctgtgtcg gcatatggat tctcctttct ttcaagatga cacattgtca
    36421 tatgacgcta tgtcattata acacctccta cctgttttgc aagacatttg catagttttt
    36481 ttcattatca tccgatacat taaaaaagtg ataagaggaa tccttccatt tgaattaatg
    36541 agagttgccc attaaaatag ccacatacta aacaaaggat gagataaatg gcccaatcaa
    36601 ttagtgtcca agacttcaat aaattgcctc tcgatgctga cagcatcgtt ttagatacaa
    36661 gaaatacctt agattacaca aaaaaccact taagccactc gctttctatc cccgctgcta
    36721 tcttgccaag acaattaaaa aatctagatc caaagaaact ttattatatt ctaagctata
    36781 caggcaggcg atcagaagca ttagccgatc aacttactgc taatggtttt cgtgctgttt
    36841 atatcattgg tgggatggaa gctcttcagt cgagtgttgc ttgaatattg cttgttagat
    36901 tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt ttataccctg tcttttttta
    36961 gttatttatt cttcaacctt atcaatatga cttgatagcc ttttcttttt cgagtaaact
    37021 gattttgtta gtcatagtta taacaatcat agcaataaaa ggagaaggta agcatatggc
    37081 atctgtttta gtagtcaaag gtcatcccct aactgcagaa gaatcgcgca cagttaaggc
    37141 attgacgtct tttttaacaa gttataaaga aaatcatcca gatgatgaag tgactgtttt
    37201 ggaactttat cgcgatgata ttccggaaat cgatgaagaa ttattatcag gttgggaagc
    37261 attacgtgct ggtgcagaat tcacttcctt atcagaaagt caacaacaaa aaattgctcg
    37321 tttcaatgaa ttgactgatc agttccttgc tgctgataaa atcgttatcg caaacgcctt
    37381 atggaatcta aatatcccaa caaaattaaa agcttggttc gataccgtca atgtagctgg
    37441 aaaaacgttc cgctatacag aaaacgggcc agaaggatta gtgacaggta aaaaagcact
    37501 gcacatccaa tctaatggcg gtgtatacaa cggacaagac tttgcctctc aatatgtaaa
    37561 aggtatttta aactttgtag gcatcgatca agtagatcag ctattcatcg aaggaatcga
    37621 ttatgaccct gaccgtgcag atgagttgat gcaaaatgca ttagataaag ctgctgcttt
    37681 aggtaaatca ttttaaaaat tgagataaat gcatacaaaa gagacagagg aaaaaatctt
    37741 caaagcagca tttgtgcctt ggatattttc ccatctgtct ctttttgtta ttctttctgc
    37801 tttttcaacg gattttcttc ataaagagaa gagattgttg tgtaccattc aaaaacatga
    37861 gtaatcacta ctttagctgc tgcataaaca ggaatcccca agatcacacc gatgattccg
    37921 aacaatttcc cagaagtcag cagaactaat aaaatcgtta ctggatggat tgctaactga
    37981 ttccctagta ctaatggaga gatcaagcgt ccttctattg tctgctctac tgcaaaaacg
    38041 atcagtactt tgatgatcat gaatggacct gctacaatgg ctaaaaagat agccggaatc
    38101 atcgctaaaa atgatcctaa gtaagggatc aggtttaaaa aacccgcaat aatccccaac
    38161 gtgacagcat actctaaacc aatgaccgca aaaccaatca taaacatgat tgccacagca
    38221 aaagccacag ttagttgtcc tcttatataa gaagaaactt gactattcat ttcactcagc
    38281 acattcaacg tcggtttttg catgcgtgtc ggtaaaaatt tgacaaaata aggtgctaat
    38341 tgtttaccgt ctttcaatag ataaaataag ataaacggca tcgtcagcag agccactaaa
    38401 atagtcgcta ccgcgccgac aaaacttcca agactctgaa ctgtactctt agatatatct
    38461 tggatcatat cgcctgccga attcatgaac ttatcacctg ctgtttccaa ctgttcgcga
    38521 aattggttga acagcgggtc ccgcaaaatc tcggttaatt tattatcaat cgtatctaca
    38581 tattgaggga agttactgat gaagctcact gtttgttcct gaatcttagg gatgatcact
    38641 acgctccccc atgcgattag tgcaacaacc aaaacaaaca gcccaatgat actgtaaagc
    38701 cttggtactt ttcgcttttc aaagtaatca actaccggat tcatcaagta atacaagatt
    38761 cctgctaaaa tgataggcag acctacgatt gctaaaaatt gccagattgg tgcaaacaaa
    38821 tatgaaactt ttgtaaataa aaagacgatc agcaatatta acaatacaac aagtaaggct
    38881 gtgactactt gattatttaa aaaccagcgc caaaaccacg ataccggtct gttcttctta
    38941 ttttttcctt ccataattcc tcctgttagt tataaaaaat ttggcttccc aaaccaatag
    39001 caggcagtct atgcggatga agatagattc catttgcaat ttgtccttct tctggacaag
    39061 catcaaaaat caatgatacg tgagctactg tctttaaatt cccattggcc atcggaccga
    39121 cataatcgat cgtatatacc tgatcatcaa aagcaattgt tccgcctgct ttcaaagaaa
    39181 aaggttcgtc ttttgatact tcttgaatca ccgaatattt tcgtaaggca gctgttgctg
    39241 ttttatcgaa caaaatcagc atgggttctt ccttgtttat tgcttctgtc ccaatttctg
    39301 taatctttgc ctgaatcatt gcactctctc ctttaattgc tttcaaaaac agtataacac
    39361 attctcattc tttagccgtg tggaataatt atgtgataga ataaaaacga aaactatttc
    39421 attacaatta ggaggaatca gctcatatga tcgaaaagat aattttagga acctatacgc
    39481 gtcgcgaaag taaaggaatc tatacgatcg accttgatac agaaaaagaa gaattagcca
    39541 acctttcatt agctgttgaa gaaaatagcc ctacctatgt agcaaaaagt aaagctggca
    39601 atctttacac agttactagt gtggatggat taggtggtgc cggtgcgtac gataaggatt
    39661 ttcatttttt gaatgcggta acagagtctg gcgctccttt gtgttatgta gcggttgatg
    39721 aagcacgtca attactatac ggggcgaact atcataaagg tgaagtaaat gtctaccata
    39781 tattaggaga tggcgaactg aaagcagcag attcaatatt ccatcaagaa gcgacaggtc
    39841 ctcataaaaa ccaagatcat gcgcatgtcc actatacgga tctgactcct gaccaacgat
    39901 tagtcgtatg tgatcttggt acagatcgtg tgtatactta tgatgtgtcc gaaaacggca
    39961 aattgaatct tgtaactacc ttcactgcag agccaggaac aggccctcgc catcttgtct
    40021 ttcatccaaa tggacaattc gcttatttat ttggtgaatt agacagcaca gtccgcgtgc
    40081 tttcttataa taaagaaaca ggtgcttttg aagaattaca gaaaatatcg accttgccag
    40141 aagaattcga cggtgaaaat ggaggggcag ctatccgcat ttcaaatgat ggcaagttcc
    40201 tttatgcttc taaccgagga cataactcga ttgctgtatt cgcagttttt gaagatggtt
    40261 cccacattca aaacaagcaa atcatctcaa cagaagggga ttttccccgc gatttcgcac
    40321 tagatccaag caataatttt gtcgtgtgtg ccaatcagaa caccgataat ctgacattat
    40381 atcgccgtca tcctgaaaca ggattactgg aattactgaa aaaagatgtc tatgtaccag
    40441 aatgtgtctg tgtttatttt gatcgttagt aagaaaagga gaatgaaatg aatcagcaag
    40501 acgtattaga cagactacgg gaagaactaa atattccttt ctttgatggg aaaatcgaag
    40561 ataaagagta tagcgaagag gaatatcaga aactcaaagc cgatttacag aactactttg
    40621 aacaatatgt acagaatgta gaaaactaat cagctttcca tactatctga aaagaacatc
    40681 aggacaagac ttttccgtct atcctgatgt tcttttctac aaataaaaaa ccgccgagat
    40741 ggttagactc gacggaaaag gagtatttac tcgattttga gtaagtacaa ggttgttgtt
    40801 ggttataggt atgtcattat cataaaggat tttgatcact ctatcataaa ggtaatatta
    40861 catttatatt agataactct agtttttatt gttatcaaag atttctttta ttttttcaac
    40921 aacaaattag tcattttttg gatggcagct gtcaattggt ctgtcgcttg gtcttgtttt
    40981 gcttcagatg tattgaatag atcttcatct acccctgatt caagatcgat actttggatc
    41041 atttcttcac agcctgctac atatgaaaga taagcttttt caaaattttt atgaatgccc
    41101 attgcttttg ctggtggacg caaagtactg actttcataa gcattgcccg atattttttt
    41161 gttccttctt caaataacag gatgatctct tcaagagtcg atttgtccag ttcacttgtt
    41221 tttccttcat caattgcgtt tctgatcact tcatatttcg gatgcattcc ttctccgatt
    41281 gcttctgttt ccttgacgat ttgatcgatg gactgcacat aatagccaat attcggtaac
    41341 atgttcgttt tcctactttc tccattgttg ttattattta ttctacttgt ttttatcctc
    41401 aaatgacaag tacttgaaaa aatttaatgg tttctataca gctcgccatt cttattcgtg
    41461 ctcaaagaaa ccatctgcta aactatattg tttccctgcg acaatgtcat attggatcaa
    41521 ttgatattcg tcgtaaatct cttgttgcga gcgattgaat tgcgcttctt tataccactg
    41581 tccgttttga tagtacagtt ctcgttcaaa cgccggaagt tcctgttcta atgataaaag
    41641 cagctgataa aagccatttg ttgtctgatt cgtctgttcc attagattcg ctgcaaaata
    41701 aaacggactg gtgattgcat cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa
    41761 cataagaaat tgtgtttcat gcaatgtttg tttgtcattt tttgcttgga tcgtatctga
    41821 atagatccct ggaagatgat ccccccaaaa aacaactaaa gtccttctag aaagattctt
    41881 caattcttcc gtgaactgct tcagtgccgt gctgctatag gaaatatctt gcagataatt
    41941 ctctaagtca agtgtcccac ttccatctga agtttttgca ggatagtcta gattatcata
    42001 ctttccatcg tagggcatat gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt
    42061 ttcttccttg agaagtgtca ttacttcttt gtaagctgac tcatcagaaa tatatggatt
    42121 attctctatc gtatccgtat aggtcatcgt acgctcgctg atgaactggt caaatcctaa
    42181 tgtctgataa acatcctctc ttttatacat cgaagtattg tatggatgga tcgctgtcgt
    42241 ctggtatgat tgtgcgttta acgctgaaac gatagaagga agctgatcca tttttggtac
    42301 aagcatcgtg taaggggtgg tgagttgcgc attaaataac gccatagaaa aaccagtcaa
    42361 tgcttcaaat tcgatatttg ctgttccacc gccatagttt tgagaaagca tattgccact
    42421 atatgtttga tcagctactt cataataatc tgccaatggc tcaccagata cttctacgcc
    42481 gtttaatcga ctaggatctg agaaactttc actcatcacg aacacgatgt tcggcgactc
    42541 ttcctctaca gttttatttt tttcatcggc tagcttctgg tatttttcag tgatttcttt
    42601 gatcttttct ttcgaatatc cttcaggttc atccattggt tctaccttca gattgtacaa
    42661 aaacccaccg ataaaaccag tgttatagta gttcattttc tggctgtaag gtatccatag
    42721 tgccgtttta ttatatgctt ttcgcaacaa attatcggga ttgttgaaat tactgatata
    42781 ccctagcagt ccaattgcca caagcagtgt cgttaatcgg atcaattgga atcgccttcc
    42841 ttttttcagg cttcgaaaca tgtaccagca aaacaatcct atcacggttc ctgcggcagc
    42901 taagatcact gtaaacagca tagtgccggt catatctttc agcaatccga tttcagtgat
    42961 catcttcaaa tcgtctggat aaatcggttc ttgccgataa ctcattttca tataatttgc
    43021 aaaacctaat acaccgatgg ctacgacata cgtaagggaa cctaaaggga cagatcctgc
    43081 tagtgcaatc agaaaaataa gaataatcag caagacgaga caggctaaga agaatttttc
    43141 cgtatgccaa gaaaaagcaa atttcaacgc aaggtctacg gatagatcat tttgacacca
    43201 ctgcaaaaat aaattactaa aaacaacaag aaaaaagata ctgatgattg ctgctgttcc
    43261 ttttaaaatc tttttctgtt tggtcgaaag tctgcttata tatcttttca aatgcttgct
    43321 ccttcatttc tatcaataaa agaatgttgc ttctcttatt ttagttgtac gaaattcgaa
    43381 agtaaagaac ggaaagaaga attaatgaaa tccaaatatt ctctttcttt atggcctttc
    43441 tggtttcttt aataagcgga cctttcgtac tccagaaatt ttttctagct gttgtttcac
    43501 ttgattgagc tgttcaaaat cagtactgtc tatgtcaatc aatatgtaga catgctcatt
    43561 cttatggtta cgcgatatat ctgcgatcgc tagctcgttc tcattcagca gagaaaatat
    43621 ctctgctaat atattggttt tcttttgata aaatatagtg aatcgaaacg gcgcttggaa
    43681 aaacattcgt gcggatggat aattgaccgc ttctctaacc gtaccgaaca aaagataatc
    43741 ttttaaggct tcaactgcca aacgtccgct gtctgccaaa gcttcctgag tcgatccccc
    43801 tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg gaaaatcagt
    43861 caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt tatcaacaat
    43921 ttcacttctt cctacattga taagtacagc atcttttttc attttttcta tattcgcctg
    43981 atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt aatccgaggt
    44041 agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat cgcggataga
    44101 ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg tgcctatcgc
    44161 tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat aagcacttct
    44221 tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca tacgacttgc
    44281 ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt ttactgcatt
    44341 agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt
    44401 gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg taagccattc
    44461 atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat ctattatttt
    44521 gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa tcaaatgaac
    44581 gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca attagacaac
    44641 tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta ggctgagtga
    44701 taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc aaaatattga
    44761 gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa gactaaattt
    44821 caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac taagtggctt
    44881 tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt ttgcccacgt
    44941 ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc cacctgcctg
    45001 aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca cacgtgcaac
    45061 aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga tcagcagttt
    45121 ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt tcccatcaaa
    45181 tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa cgattcttgg
    45241 tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga ttgcttcttg
    45301 gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc ctcgagccat
    45361 gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag caaaaccgta
    45421 tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc catacaggtc
    45481 ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc gaataccgat
    45541 ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat aatcaccata
    45601 gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt cgtctgatac
    45661 gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc aaccaaattg
    45721 aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt tgtcaccaaa
    45781 acgagccact tttattccaa aactttcatt ataggctact gcaacatcca tccaatcagc
    45841 aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat tttgtttatt
    45901 taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat ttaaattcat
    45961 gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag ctaaatgaag
    46021 caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg agaaagtatg
    46081 catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca tgatttttgt
    46141 gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca attggccatt
    46201 ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca gtgcttcttc
    46261 cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa tattcaacat
    46321 aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt gttttcgtaa
    46381 ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga gctgtctggt
    46441 aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg cactttgcgc
    46501 atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa ttgctaaggg
    46561 atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat attcgtaccc
    46621 gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat aaaaagtatc
    46681 tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga tcgtcgccca
    46741 tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata aaacagcatg
    46801 agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt ccaagtccac
    46861 aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt tgataacgaa
    46921 atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga cgagtccgtg
    46981 tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca gcattttcta
    47041 tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc ccctcacggt
    47101 aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg atggtgattc
    47161 cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc ccatgtcgat
    47221 ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg gctgtgtcca
    47281 tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact tctggtgttt
    47341 taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat ttcaatatat
    47401 ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg aaattcgcta
    47461 aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt actcctgtaa
    47521 tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta tcgccttcta
    47581 gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata cccaaagaat
    47641 tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag ttattcgtat
    47701 gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc ggatggacat
    47761 tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc gaaatgttcc
    47821 ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc ccttctggcg
    47881 gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa gcccctgatt
    47941 cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg tgatccaagt
    48001 caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt tcttgatggt
    48061 aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg cctagtactt
    48121 tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa ttaatttctt
    48181 tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc catctctgcg
    48241 gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg attgtatttc
    48301 gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga taaccgtgca
    48361 tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag atttcgaata
    48421 cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct tctaatgaat
    48481 aggtccagat accattttct agtttgtttt cccactcata actaccagcg gcaataggag
    48541 taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca atccctaaag
    48601 atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc ataatgattt
    48661 aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct tacaatgatt
    48721 attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa taaaaaacac
    48781 ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga tcaaccgcct
    48841 tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt tcctcatcgt
    48901 ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt ggtgttatga
    48961 ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct tctttgatag
    49021 gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta tctggtcttc
    49081 tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt tttatggctg
    49141 gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt gcagcaagga
    49201 ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat atgtctgaaa
    49261 tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg attgcttctg
    49321 gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa acgatggctt
    49381 ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc ggtgttttag
    49441 ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag gcaaaaagag
    49501 tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa atccagctga
    49561 atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta gaaaaatatc
    49621 gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa ggagctaatg
    49681 ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca gcgagcgatg
    49741 cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg ctctttttgg
    49801 ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc gtgatgggac
    49861 tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca aattcgttgt
    49921 tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg gctccactga
    49981 catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca tctggcctag
    50041 cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct gtgatgactg
    50101 cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt ttaggggttt
    50161 tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa atcgaacaaa
    50221 gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa agaggttgtg
    50281 acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt cacaaggaac
    50341 aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc agttggtagg
    50401 agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg atgacaaaag
    50461 ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa aaacccgtct
    50521 cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca tagagaatat
    50581 ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca tcttgtccca
    50641 acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg cttaaagaag
    50701 aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt tctgtcaact
    50761 tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct aaagcttgtt
    50821 cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc gtggtgaaag
    50881 taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg ataaaacctt
    50941 tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga cgctgatgtc
    51001 ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct aaacaaatat
    51061 aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg ccttgttctc
    51121 tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta ggctcaacga
    51181 tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc aattcgtgat
    51241 cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca ttttcgattc
    51301 ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca ccgatcgttt
    51361 ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat gtgcccgacc
    51421 ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc cgaatcgtat
    51481 gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt gtatttactt
    51541 cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga tattttgcca
    51601 tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc aaggtttatt
    51661 caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc accaaattct
    51721 ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct
    51781 aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaaat atcaaagcac
    51841 ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt tttttcagaa
    51901 gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag attcttgtgc
    51961 tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc agaatacgta
    52021 atgaatattt tcccattgcg agtaaggact gctggacctt catttactga aaaaccccgt
    52081 ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa cgtccaagga
    52141 ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc ttgttgggcc
    52201 cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact gaaagtttcc
    52261 cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc tatatgctga
    52321 ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt atgattgctt
    52381 gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc ccagatcaat
    52441 tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc atgttctaat
    52501 tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac cgatcctgta
    52561 aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg ccgaacgata
    52621 ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt attctaatta
    52681 cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct attgaaaaat
    52741 agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc aacagatcgc
    52801 tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta
    52861 tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg ccgttatcta
    52921 cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa acagggcaaa
    52981 tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc atcacataat
    53041 aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt attgtgttta
    53101 ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata gccagcccta
    53161 atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg ggactaccga
    53221 cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca tcaaacgcta
    53281 gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta gcaacgattt
    53341 cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta tcatcacgct
    53401 catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag ttatgcaaag
    53461 ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat tcagggtacg
    53521 atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca tccactaact
    53581 tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc ttagccccca
    53641 cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca aaaggctttt
    53701 tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa ttacaatatt
    53761 ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg aatatcggtt
    53821 tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa tttggttcta
    53881 tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct acagtatcca
    53941 cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct aattctaatt
    54001 cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt tcatggcccg
    54061 gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag ccaaacagcc
    54121 gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact tcttcgtcca
    54181 acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt ttccatacgc
    54241 caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca aatcctaata
    54301 acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat gcactttgtt
    54361 caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc caggataaaa
    54421 gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg attgtctgtt
    54481 cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta aggaacaagt
    54541 tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt cctgatccct
    54601 cacctttcca ttgcgccatt acataaaaaa acagcacatg tagcaggcac gatagtaata
    54661 acaggcaaac acacgattac ccagagaata cctaataaga tgtaatcaga aaccacaatc
    54721 aactgtccat aaagtttagt ctcaactatt ttcatcttcc tcactctttc attccagaca
    54781 tagctgttcc ttgaacaaaa tatttctgtg caaaaatata aaccactagt aatggcagca
    54841 ttgaaataac cgttccagcc attacaggtc cataaaaaga aatgttctgc cctgtaaata
    54901 aagctaaccc tgctggtaaa gttctcattt ttgaactact tgtcaatatc aatggataaa
    54961 gcaagtcatt ccaactattc atcagcgtga aaatccctaa tgataaaaga gtaggtttgc
    55021 tcaatggcaa catgattttc aaaaaaatat taaactcact taatccatca atgcgtgctg
    55081 cctcttccaa agaatctggt aaagtaataa aaaaggaacg catcatgaag ataccaaatg
    55141 cagttgtcat acgaggaatg atcaaacctg cataagtatc aagcatgcca agcaaattga
    55201 cttcaataaa tagaggaatc atgaagactt ggaatgggat catcattgtc agcaaaacga
    55261 aatagaacaa aactgatttt cctttgaaac gcatacgagc aaatgcatac cccgcaagag
    55321 aatcaaagaa tacagaagtg atcactgtcc ctccagcaaa aatcactgta tttttgatgt
    55381 aatctaataa agggatcgtt gtccaaacct taatatagtt atctagggta tagctatctg
    55441 caaatattct aggcggaaat gagataatgt ctttttcgtg tttaaaagaa gaaataatca
    55501 gccacaaaaa aggaaacgca ataaccaaca atataactag catactaatc gttcgaatga
    55561 tttttttacc actaactgct tttcgactat tcacagtaac tgtaatcgag ctttctggtg
    55621 ccatacaggt ttccctccta tttcatttgt ttttctttcc gtgacatgta cacattagtg
    55681 gagatagcta tagttgcaac aatgataaac aaaaccgaag acagtgctga tgcatagccc
    55741 aattcataag gtgctgtaaa tcctcgactg taaatatatt gaacagctgt ttctgtctta
    55801 aattgtgggc caccagcggt tgcaacatat acttggtcaa acacttgcat cgaaccaatc
    55861 agattcgtta aaatacaaaa acctaaggag ggaacgatct gaggtagcgt aatgttaaaa
    55921 aactgctgtc gatttgttgc accatccatt tgagcagatt catacaaaga gggagagatt
    55981 ccctgaatag cagtcaagag aatcgtcata gtaacaccaa aattcttcca aacagtcatg
    56041 atggctaccg tcggcatagc aagggttgga tcgcgaaaaa attgagggat cggcaagccg
    56101 acttgtgaaa acaagtaagg aatcgcccct acattcggat caagcaacat agaaaaaata
    56161 atcgcaattg acgtcaatga acaaataact ggaatgtaaa atgtcgaacg acaaaaacga
    56221 ttgaatctgg tatttttaga taatgctact gctgctaata aaccaacgat aatttgtgtt
    56281 ggcgtttcga gtacggtgaa atataaagaa tgaatcaacg aattaactgc tcgttcatca
    56341 ctaaatatac gtgtgaaatt ttcaaaactt acaaaatttc ttgatgtaaa gaaaatattc
    56401 aaatctgtaa aactaatagc aaatgttcct aaaagcggta tgacagtaaa taataataaa
    56461 actaaaaaag atggagttaa aaacaaatac gcagcacgat caggacgatt ccaatatttg
    56521 gtgaatccgt tcatatagtc cacctcatct ttcattttga taaaatatac aatcttggtt
    56581 taaacgaatc tgaataagat atccgaacaa tcgctgtgat ttttgcaata acacctatct
    56641 gcaaatacta ggtcgatcat tcagatatct gtatcttgcc aacagtctat cctgtataaa
    56701 gttctgtact atttttgcag taattgattg atggcatcat ctgcatcgtt catgagtgtc
    56761 tcaggatctt ctcctgctaa taatttttca atcagtggat tgatgatatc gttattgatc
    56821 gcagagattt gggtaaaacc tggtaaatat ggctctgcat agtccatttg tttcgataat
    56881 tcagatacaa taggatcatt ttttacttct tcatcttttg ctactgaatc caagtaggca
    56941 ggaaaaccat tttcgatact ccatttttta ccaatttcag ttgtattcca atatttcaaa
    57001 aactcataga cagcttcttt tttattatta tctgtacttg ccggaatggc atatcctacg
    57061 ccatctaaaa tagctgtttt tttgtctgaa tcactccctt gtggtaaggt agtcacatta
    57121 taatcaatct cgtttgcttt caatccgtta tttaaccatg gcccattgat ttcaatggct
    57181 agttgaccag cattcattaa attgtccatt tcggcgccag aaatcgattc tggaccagtc
    57241 ttgtctacat ggatcatttt ttggatcgct tttaacgttt taaggttctc aggagacgca
    57301 aattcagact tagtataatc atcgttcact aatttaccgc catttgctaa catccagtta
    57361 tataacggtg cagttccatc cttattgaag acaaaacctg atacattttt actactatca
    57421 gttaattttg gtgccatttc tgtcaattct tcccaagtag taggtggttg ttcagtatct
    57481 aatccagctt gtttaaacaa atcattattc caatacatat acatgccttg tacctgcatg
    57541 gggacaaaat actgtttacc ttctacttca cctaattcaa tagccgtttc tttaaaatca
    57601 gatttatcta ctccatcata ttcccagaaa tcgtctaatg gttgtaccgc tccgttttct
    57661 acatactgtg caaaatctgg ataatttaat gcaataaagt caggtgcttt tttagaagaa
    57721 atcgctgtag gaagtttttc attcaaattg gcccaagtca tgatatccat ttcaattttg
    57781 atgtttttat catttgtttt attaaattca ttaacgattt cttgcaatat ttcaccatca
    57841 gatgcagtaa acccattcca aaaagtcaaa gtcacttctt tatcagaatc agattctgct
    57901 gaacttccgc caccgcaagc tcctaaaata cctgttgcag ctaccaacaa agagccaatt
    57961 aaccattttt tacctttcat tttttgttcc tccttcatct gataaacgac atctgaccac
    58021 attccaagat agcggtttca ataaacaatt gattttccca tcttcagctt ttacatttgt
    58081 tgaatgactt ggttttactt gttcactttt aggaagatta gttgcttttg tatcaaatcc
    58141 actcatttca gaaaaatcaa tcactccctc taatgataat tcagacaagt gaacagttag
    58201 attcatttct tcttctccac ggttaactgc aaatatgaca agttctttgt catttattac
    58261 ggctatactt tctaaatacg gcacttcttc aaaatcttcc gtcgcatagt ttgctgaacg
    58321 aatttgtggg cttaacacct ctccttgtcc atacacagca gcttgcataa atggataata
    58381 gatcgtctgt ctccaagctt ctccgtttgt ttctgtcctg attggtgcaa taacatttac
    58441 caattgagca agacaagcaa tctttactct atctgcatgt ttcaataaag tgattaatag
    58501 acagcctaat agtaatgcat cttcaaaatt atagttatct tctagtaaag gaggagcaat
    58561 ctgccaaggc gctgcttgct catcttgttc cgctgaatga taccaaatat tccattcatc
    58621 aaaggacagg ttgatctgct tcttactatg tttcttcgcc ttgactgtat cgcacatcgc
    58681 tgtcacttca gaaatgaacc gatccatgtc taaggaacgt gcaagataat gagaaagatc
    58741 atttttttgg ttgccataat attgatgcag agacaaataa tctatttgat cgtacgcttg
    58801 atcaagaact gttaactccc aatcaccaaa tgtaggcatt tgccgattcg aacttccgca
    58861 taaaaccacc tcaattgaat catctacgag tttcatcgcc ttagccgttt cctctgctaa
    58921 ccgtccatat tcttccgctg ttttatgtcc aatctgccaa ggaccgtcca tttcattccc
    58981 taaacaccat gtcttaattc caaaagggct ctcttgacca ttcgaacgcc ttaaatcact
    59041 ccaataagtt cctttaggaa aattacaata ttcgactaaa ttacgagctg catctactcc
    59101 acgagtaccc agattgacag ccatattgat ttcggccccc acatgctgac accagttata
    59161 aaattcatga atcccaactt cattcgtttc aattgtccgc caagctaaat ccaaccttct
    59221 aggtctattt gctatgggcc caatactgtc ttcccaacga aatcctgaca caaaatttcc
    59281 accaggatat ctaatcatgg gaatgttcaa ttcttttacc aagtgaatga cgtcttggcg
    59341 aaatccactt ttattagctg atggatgatc tggctgataa atcccttcat atactgctcg
    59401 tcccatatgt tcaataaatg aactgtacaa ccgcggatca actttgccta cacgcttgac
    59461 actactaatc aacattgtgg cttccataac tgacctcctt taatttatac atgaataata
    59521 cacctcgttg aaagcgttgt caataatata aatgttaaac ttacttattt tttatcgatt
    59581 ttaattaacc aaaatattaa taatgattaa aacaaataat ttaatataac ttttattaac
    59641 aaatatgtta atatataaga aaaacctttt aagaggtgat atatatgcaa ctacaaataa
    59701 acgaagaatc cttaccggta tatgaagcat tagctagtaa aacaaggatc aagatcatcc
    59761 agctactttc caaaaaaaag atgaatgtca aagatttggc aaaagaactt ggagtgagca
    59821 gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc aaacatcatc aaaacagaaa
    59881 aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa aatcgatatc agttttccag
    59941 aaaaaatatt caatgctttt gatacgaaag aaacatcgat acctattggt cactatacaa
    60001 attatgcaat tgaaccaaca tgcggtttag ctacgatcca tgattttatc gggaaagtgg
    60061 atgaaccacg ctattttatg gatcctcgaa ggatggatgc ccgcattctc tggtttacca
    60121 gcggatttgt cgagtatcag gcacctaatt ttttaaatac agaggataca ttggagatgt
    60181 tggaagtttc tgtggaaatt tcttctgaat ttcctttttc aaatgacaat tggccttcag
    60241 atatcacttt cagcctaaat ggtgtggagc taggaacatg gacgagccct ggagattttg
    60301 ctgatatccg cggtaaatat acgcctgatt ggtatccaga taatcttaat caatacggct
    60361 tgctgaagac gatccgaatt accaaacact tgacgaatat gaatggagaa cccttgtcaa
    60421 acatcacgat caacgacatc cctaaagagc aagacacttg gcatttgcga atcgaagtga
    60481 aagacgatgc caaacatgtg gggggctgta ctttatttgg taaaggattc ggtaattacg
    60541 atcaggatat caaattaaaa gtttactaca gctgatattt ttcttttcta ttttataaaa
    60601 caaaaacagc caatccctat gaagggacgg ctgtttttgc tttattgtta tttccagaaa
    60661 tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg tcttgttcca ccatgcttcg
    60721 attttttctt gtgcttgagc gggaacttct tttccttcta gataatcatc aatttcatta
    60781 tatgtgacac ctagtgcgac ttcatctgca atcattggtt tcccatcttc aagatctgca
    60841 gtcggaattt ttgtgtataa tttttccggt gcattcaatg cttgaagcaa ttgtttccct
    60901 tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc caccatcacc aaatttcgtg
    60961 aagaatccag taatgttttc tgcagcatga tctgtcccta atacggctcc tgctctttct
    61021 ccagctacgg catattgcgt aatcatacgt tgacgtgctt tgatatttcc tttgttaaaa
    61081 tcgctgattc ccacacctgc ttcgcttaat acttgtactt gtgcatcaac agctggtttg
    61141 atatttactc gcagactaac atcgggctgg ataaagtcaa gagcagcttt tgcatcttct
    61201 tcatctgctt gttcaccata aggaagacga acagcgataa attgatagtt ttcatctttt
    61261 gtttcttcac gcatttcttc catcgttagt tgcgccaaac ggcctgctaa agtagaatct
    61321 tggccgccgc tgatacctag tacaaaagtt ttaagaaacg gatatttttt caaatatgct
    61381 ttcataaagt caatactttt acgtatttct tcttttggat caattgttgg tttgacacct
    61441 aattcattga taatttctgt ctgcaggctc atttattctc ctcctatgct tctgtatgaa
    61501 gttctgctac gctttttttc attcgttcca tgatagccat tttatggttc cagcattcag
    61561 tagacagatc gactgggtat ttttgcggat tcaagtctcg tttatactct tcccatagtg
    61621 aatctagatt ttcttttgta tattctttga tttcgtttag tgcaggtaat tcgtaaactc
    61681 tttttccttc gataaagata tcttgtaaaa ctgggcgagc tgtaaagtct cgaacagttt
    61741 tattgataaa cgtatgcact ggatggaaca tgaagatagc ttcttcttct cgagggtctt
    61801 catcccataa tgtcacgtag tctccttcag atttcccatc aaaatttcga gtgatgcgcc
    61861 acacttgttt tttccctgga gtagttactt tttctgcatt gcttgataac ttgatcgtat
    61921 ccatcatttt tccttcatca tcttcgatcg atactagctt gaatactgca ccaagggctg
    61981 gctgatcata ggctgtgatc aattttgtcc caacacccca tacatcaatt ttagcttttt
    62041 gcatctttaa attcaaaatc gttgcttcat ccaagtcatt cgaagcatag atttttgctt
    62101 ccgtaaatcc ggctgcatct aattgttccc gaacacgctt agatatatag gccatatctc
    62161 cactgtcgat gcgtacgcct tggaagttga ttttgtcacc taattctttc gccacgcgaa
    62221 tcgcacttgg cacacctgat ttcaacgtat cataagtgtc gactaagaac acgcagtctt
    62281 tatgggtttt agcgtaggcc ataaatgctt cataatcatt tccataagat tggactaacg
    62341 aatgagcatg ggtcccgcta gctggtatcc caaatatctt cccagcacgt acattgcttg
    62401 ttgcatctgc tccgccgata tatgccgctc tagttcccca gactgctgca tccagttctt
    62461 gtgcccgtct agttccaaat tccaatagtg gatcttctcc gatgacagat ttgatacgag
    62521 ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt caacagtgct gtttctacta
    62581 attggcagtg cgctaaaggc ccttccactt ggattaatgg ttcatttgca aaaactagtt
    62641 ctccttcacg agcagaacga actgttgctt taaattcaaa attttgtaaa tactccaaaa
    62701 aaccttcagg atagacttct aatccacgca agtaagcaat atcggaatca ttgaacgtca
    62761 gattctctaa ataattgact aaccgttcca atcctgcaaa aaccgcgtat ccgtggttaa
    62821 aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa atcagctcga cctaattccc
    62881 agtatgtctg catcatattg atttgatata gatccgtatg cagcgtcaaa ctgtcgtctg
    62941 ggtaagtatt attcatcttg tattactcct cttggcaagt tttcatttcc tacccattat
    63001 aacaaaaaaa attaatagga aaagaaatct tccatttttt ctgaaaaagc acatgtttta
    63061 cggatatttc ttgctaaaaa tgaactccat ctgctaaaac tatcctttct tattggtatt
    63121 taacctaaac gagaaagaat acttatttta ttcaatgtat ccgagacacg aagaaattag
    63181 cgttttctat ttttcttact tgataaaata agtagatttt cctactacaa tcgtagccgt
    63241 taagataccg atgattgaac aaatataacc aaatgtctct acaacataat caatggcaaa
    63301 agtttttgaa gcaataatac gtctttcctt ttttgcttct tatccccaaa gaaacggata
    63361 taacttatta tgtattgaat ttttttggaa aggcgaatta tgacaagata taccttgcgt
    63421 tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa gatacaaagt aagaaagcag
    63481 ctgtcaatag aatggaaaca aaaatggcga gacatcaatc agtctcgcct tgttttttat
    63541 tgtatacatt agatatggag caggttacca taaagatgat tattagtcgt tattaaatag
    63601 aatccttcgt atttttttgt tctatcacta caatatcttt atttggacgt ttgaattgta
    63661 ttccggcacg ttctagctcc tgtttcactc ctacatggac tttgacatct tctcgtgtca
    63721 tttttaaaac gtgatgaatc gcatagtcga tttcttgcct tgtgattttt ttcttcgtat
    63781 tgatcatcac gacttgcttt ttatcatatg tgtctaccaa tagcagttgt gttcgatcta
    63841 acgaaaataa tttgtaataa ttaatagccg tattcccaaa aaagaattcc actactttag
    63901 gataaaatgt cttaatattt aaattactat tcacaggtgt ttcgattagc ttcatctcat
    63961 tcctcttttc tatatagtcg atttagatcc aatcttaagt tatgttacca tctttcctag
    64021 cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa ggatttacct tttatcactt
    64081 aatagtataa cataatttcg tgagaaaaaa atacaatttt ttcttgacca tctcgttacg
    64141 taaaggatta tgcttacatt tgtcaggagg aaaaataatg gagtacacaa taaagaaaat
    64201 gtcagagata tcaggagtca gtccgagaac cttgcgcttt tatgatgaga ttggactgct
    64261 aaaaccagct aggatcaatt cttcaggtta tcggatttat ggcaaaaaag aagtcgatcg
    64321 tctgcaacac atcttatttt atcgaacaat ggcattcaag ttggatgata tccaagaggt
    64381 cttagacaat ccatcttttg accaccagaa agctttaatc aagcatcagc aaatgttgct
    64441 ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc caacagacac ttgatatgta
    64501 cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa gggtttaaac aacaaaagtt
    64561 gaaagaaaat gaagaatcct atggacaaga gattcgacaa aaatacggaa aaaaaaaggt
    64621 tacagcatcc aatgaaaaat ggatgaatat ggataaagaa acgtatgaag aaatgcaaac
    64681 tgttgaaaag cagatgttat ttgatctcac tctttactta aaagaaccag aagatgaaag
    64741 cttagcagac cgtatcttcc aagctcataa gaagtggttc tcttacagct ggcctgaata
    64801 tcagccaaaa gcgcataaag gactaggaat gatgtacgaa gccgatgaac ggttcactgc
    64861 atattatgac gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa ttatccaaaa
    64921 aaaggcgtaa tcttttacat gatccaacaa aaaagtacac gtaaaatgac ctaataacaa
    64981 aacagcagac acccaagtta gatttttatg ctaacttttg gtgtctgctc cattctttca
    65041 caagtttttt catcctactt taaatatatt catcgttgaa taaacgtata aggtattttc
    65101 actttatcct ttttttctgt aagaaacaat tgaaatgcca tttctccgca agcttccaga
    65161 tggtggtcga tggttgggaa accaagaagc tcgctgatca gcaaattatc gcgtccaatc
    65221 accactggtg tcttatcttg cagatagctt tgcaagatgc ctgctgcaac catatccgaa
    65281 tttgtaaaga caccatcaac tccaagctct ttaaaaaaga agcctgcttg ttttcctttc
    65341 tctgcatcta tacaatccca aaaaacaagt gattcatcaa aagatggaaa aagttgatga
    65401 catagctgca aagtgatttt tgagttataa cttagcctgc cacttcgccc caacgtgatg
    65461 cccaattgct ttataccttg ctgcttcaaa taagtaagac cttctgtaat tgattgttct
    65521 cgatcaataa agacacagct tgcctgtttt cccttgattt cttcacaaaa aactagaggt
    65581 ccatattttt gatatgccaa taaatcatct agtgtattgg cacgagacgt aatgatcaat
    65641 ccatcgaaag ctttagctgc aaattcttca agataccttc gctctttttc tttatgatag
    65701 cccgtcggta aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat gatcccactg
    65761 atcaactgat caaaatacgg atgattggta taaggcagaa tcactccaat gcttttcgtt
    65821 tttccataac ttaactgtct ggcatttgca ttagggatat aatcgacttc tttcatgacc
    65881 gcaagtattt ctttcctttt ttcctcatct acatatggat gtccattgat tacacgtgaa
    65941 atagtagaaa ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc cactgctgct
    66001 tcgccaccta tacaaaaaga ttttcttgta aatagtctac cacagctgga ccgcattcaa
    66061 aaaactgata catttttcct tcatgcgaaa aactgatccg ctgcccgccc caatatatat
    66121 cttttgtcaa attttcgatt tcattcattg gtatttcttt tactatattc ctattcaacc
    66181 cttgaaaact gtacaacgtt aatacggatc ccttgaactc gacaaacgca taaaaataat
    66241 tgttcgttgt gccatctgtt atctctacat aagtattcat tgcaagccct ccttttgata
    66301 ctttgagtat agtgtatgaa acgcgtctca tagcaaggag tttgttttgc caaagaaaag
    66361 ttttattcct ctttcattat taccaaagtc tcttatttta aattgaatac attataatga
    66421 tactgtaaat atatctttat tattagtgaa tg
//
LOCUS       DAYLZO010000004        61960 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.004, whole genome shotgun sequence.
ACCESSION   DAYLZO010000004 DAYLZO010000000
VERSION     DAYLZO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.004"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..124
                     /locus_tag="ACGVJG_000205"
     CDS             <1..124
                     /locus_tag="ACGVJG_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFJ6556193.1"
                     /translation="DITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            134..751
                     /locus_tag="ACGVJG_000206"
     CDS             134..751
                     /locus_tag="ACGVJG_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFJ6556194.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            769..1452
                     /locus_tag="ACGVJG_000207"
     CDS             769..1452
                     /locus_tag="ACGVJG_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFJ6556195.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            2135..3418
                     /locus_tag="ACGVJG_000208"
     CDS             2135..3418
                     /locus_tag="ACGVJG_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6556196.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            3434..4321
                     /locus_tag="ACGVJG_000209"
     CDS             3434..4321
                     /locus_tag="ACGVJG_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6556197.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            4338..5174
                     /locus_tag="ACGVJG_000210"
     CDS             4338..5174
                     /locus_tag="ACGVJG_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6556198.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            5177..5800
                     /locus_tag="ACGVJG_000211"
     CDS             5177..5800
                     /locus_tag="ACGVJG_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556199.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            5797..7089
                     /locus_tag="ACGVJG_000212"
     CDS             5797..7089
                     /locus_tag="ACGVJG_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFJ6556200.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      7448..7545
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7661..8242
                     /locus_tag="ACGVJG_000213"
     CDS             7661..8242
                     /locus_tag="ACGVJG_000213"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFJ6556201.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            8245..9552
                     /locus_tag="ACGVJG_000214"
     CDS             8245..9552
                     /locus_tag="ACGVJG_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFJ6556202.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            9968..10456
                     /gene="purE"
                     /locus_tag="ACGVJG_000215"
     CDS             9968..10456
                     /gene="purE"
                     /locus_tag="ACGVJG_000215"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFJ6556203.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            10449..11573
                     /gene="purK"
                     /locus_tag="ACGVJG_000216"
     CDS             10449..11573
                     /gene="purK"
                     /locus_tag="ACGVJG_000216"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFJ6556204.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            11586..12881
                     /gene="purB"
                     /locus_tag="ACGVJG_000217"
     CDS             11586..12881
                     /gene="purB"
                     /locus_tag="ACGVJG_000217"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFJ6556205.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(13003..14391)
                     /gene="ltrA"
                     /locus_tag="ACGVJG_000218"
     CDS             complement(13003..14391)
                     /gene="ltrA"
                     /locus_tag="ACGVJG_000218"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFJ6556206.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(14960..15337)
                     /locus_tag="ACGVJG_000219"
     CDS             complement(14960..15337)
                     /locus_tag="ACGVJG_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556207.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            15593..16321
                     /gene="purC"
                     /locus_tag="ACGVJG_000220"
     CDS             15593..16321
                     /gene="purC"
                     /locus_tag="ACGVJG_000220"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFJ6556208.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            16321..16575
                     /gene="purS"
                     /locus_tag="ACGVJG_000221"
     CDS             16321..16575
                     /gene="purS"
                     /locus_tag="ACGVJG_000221"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFJ6556209.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            16577..17248
                     /gene="purQ"
                     /locus_tag="ACGVJG_000222"
     CDS             16577..17248
                     /gene="purQ"
                     /locus_tag="ACGVJG_000222"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFJ6556210.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            17249..19471
                     /gene="purL"
                     /locus_tag="ACGVJG_000223"
     CDS             17249..19471
                     /gene="purL"
                     /locus_tag="ACGVJG_000223"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFJ6556211.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            19456..20895
                     /gene="purF"
                     /locus_tag="ACGVJG_000224"
     CDS             19456..20895
                     /gene="purF"
                     /locus_tag="ACGVJG_000224"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFJ6556212.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            20918..21970
                     /gene="purM"
                     /locus_tag="ACGVJG_000225"
     CDS             20918..21970
                     /gene="purM"
                     /locus_tag="ACGVJG_000225"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFJ6556213.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            21967..22545
                     /gene="purN"
                     /locus_tag="ACGVJG_000226"
     CDS             21967..22545
                     /gene="purN"
                     /locus_tag="ACGVJG_000226"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFJ6556214.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            22542..24077
                     /gene="purH"
                     /locus_tag="ACGVJG_000227"
     CDS             22542..24077
                     /gene="purH"
                     /locus_tag="ACGVJG_000227"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFJ6556215.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            24093..25367
                     /gene="purD"
                     /locus_tag="ACGVJG_000228"
     CDS             24093..25367
                     /gene="purD"
                     /locus_tag="ACGVJG_000228"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFJ6556216.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            25489..26403
                     /locus_tag="ACGVJG_000229"
     CDS             25489..26403
                     /locus_tag="ACGVJG_000229"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFJ6556217.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(26536..28101)
                     /locus_tag="ACGVJG_000230"
     CDS             complement(26536..28101)
                     /locus_tag="ACGVJG_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556218.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(28094..29545)
                     /locus_tag="ACGVJG_000231"
     CDS             complement(28094..29545)
                     /locus_tag="ACGVJG_000231"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFJ6556219.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(29559..30575)
                     /locus_tag="ACGVJG_000232"
     CDS             complement(29559..30575)
                     /locus_tag="ACGVJG_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6556220.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            30795..31883
                     /gene="chvE"
                     /locus_tag="ACGVJG_000233"
     CDS             30795..31883
                     /gene="chvE"
                     /locus_tag="ACGVJG_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFJ6556221.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            31937..33463
                     /gene="mmsA"
                     /locus_tag="ACGVJG_000234"
     CDS             31937..33463
                     /gene="mmsA"
                     /locus_tag="ACGVJG_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFJ6556222.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            33465..34670
                     /gene="mmsB"
                     /locus_tag="ACGVJG_000235"
     CDS             33465..34670
                     /gene="mmsB"
                     /locus_tag="ACGVJG_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFJ6556223.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            34927..35904
                     /gene="guaC"
                     /locus_tag="ACGVJG_000236"
     CDS             34927..35904
                     /gene="guaC"
                     /locus_tag="ACGVJG_000236"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFJ6556224.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            36168..37592
                     /locus_tag="ACGVJG_000237"
     CDS             36168..37592
                     /locus_tag="ACGVJG_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFJ6556225.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(37634..37891)
                     /locus_tag="ACGVJG_000238"
     CDS             complement(37634..37891)
                     /locus_tag="ACGVJG_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556226.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            38053..39162
                     /gene="pdhA"
                     /locus_tag="ACGVJG_000239"
     CDS             38053..39162
                     /gene="pdhA"
                     /locus_tag="ACGVJG_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFJ6556227.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            39165..40142
                     /locus_tag="ACGVJG_000240"
     CDS             39165..40142
                     /locus_tag="ACGVJG_000240"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFJ6556228.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            40167..41810
                     /locus_tag="ACGVJG_000241"
     CDS             40167..41810
                     /locus_tag="ACGVJG_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFJ6556229.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            41818..43224
                     /gene="lpdA"
                     /locus_tag="ACGVJG_000242"
     CDS             41818..43224
                     /gene="lpdA"
                     /locus_tag="ACGVJG_000242"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFJ6556230.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            43386..43661
                     /locus_tag="ACGVJG_000243"
     CDS             43386..43661
                     /locus_tag="ACGVJG_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFJ6556231.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            43667..44434
                     /locus_tag="ACGVJG_000244"
     CDS             43667..44434
                     /locus_tag="ACGVJG_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFJ6556232.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            44602..46431
                     /gene="typA"
                     /locus_tag="ACGVJG_000245"
     CDS             44602..46431
                     /gene="typA"
                     /locus_tag="ACGVJG_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFJ6556233.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            46692..48464
                     /gene="recQ"
                     /locus_tag="ACGVJG_000246"
     CDS             46692..48464
                     /gene="recQ"
                     /locus_tag="ACGVJG_000246"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFJ6556234.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            48578..48880
                     /locus_tag="ACGVJG_000247"
     CDS             48578..48880
                     /locus_tag="ACGVJG_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFJ6556235.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            48883..50046
                     /locus_tag="ACGVJG_000248"
     CDS             48883..50046
                     /locus_tag="ACGVJG_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFJ6556236.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            50072..53500
                     /locus_tag="ACGVJG_000249"
     CDS             50072..53500
                     /locus_tag="ACGVJG_000249"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFJ6556237.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            53521..54645
                     /locus_tag="ACGVJG_000250"
     CDS             53521..54645
                     /locus_tag="ACGVJG_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFJ6556238.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            54626..55072
                     /locus_tag="ACGVJG_000251"
     CDS             54626..55072
                     /locus_tag="ACGVJG_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFJ6556239.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            55056..55394
                     /locus_tag="ACGVJG_000252"
     CDS             55056..55394
                     /locus_tag="ACGVJG_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFJ6556240.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            55513..56073
                     /gene="rsmD"
                     /locus_tag="ACGVJG_000253"
     CDS             55513..56073
                     /gene="rsmD"
                     /locus_tag="ACGVJG_000253"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFJ6556241.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            56070..56561
                     /gene="coaD"
                     /locus_tag="ACGVJG_000254"
     CDS             56070..56561
                     /gene="coaD"
                     /locus_tag="ACGVJG_000254"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFJ6556242.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            56551..57594
                     /locus_tag="ACGVJG_000255"
     CDS             56551..57594
                     /locus_tag="ACGVJG_000255"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFJ6556243.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            57753..58481
                     /locus_tag="ACGVJG_000256"
     CDS             57753..58481
                     /locus_tag="ACGVJG_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFJ6556244.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            58597..58842
                     /locus_tag="ACGVJG_000257"
     CDS             58597..58842
                     /locus_tag="ACGVJG_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFJ6556245.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            58839..59057
                     /locus_tag="ACGVJG_000258"
     CDS             58839..59057
                     /locus_tag="ACGVJG_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFJ6556246.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            59193..59732
                     /locus_tag="ACGVJG_000259"
     CDS             59193..59732
                     /locus_tag="ACGVJG_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFJ6556247.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            59725..>61960
                     /locus_tag="ACGVJG_000260"
     CDS             59725..>61960
                     /locus_tag="ACGVJG_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFJ6556248.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPV"
BASE COUNT        19929 a        10709 c        13724 g        17598 t
ORIGIN      
        1 agatataact aagatcacat atgaaccttg gcacttacgt tatgtcggta aagaaaacgc
       61 tgagtatatc accaaacacc aattgacgct agaagaatat ctgaatgaat taaatgaaaa
      121 gtaggaattg atcatggcga aaaaaaagtg gaaaaagcaa aagcgcaaga atatccgttg
      181 gccagcagtg gttgccggtg tagcagtgat actcttagcc tttgtttttt ccttgaagaa
      241 tctgtcttct ccttttactg ataatcagca agaaaatgaa gcgcaatccc atcagcaatt
      301 tattgaacgg cttgttccgc atgcccaaga actacaagat ggttatggag tgcttcctag
      361 tatcatttta ggacaagcta tattagaatc aaattgggga aaaagtacct tggctagcaa
      421 atacaataat cttttcggga tcaaggctta tggggatcag aagaaagtaa gcctggaaac
      481 aaaggaattt gttaacgaag aatggataac tatccaagga gacttcaaag tgtatgattc
      541 atgggaacag tccatggatg accatacaca actgttcgtc caaggagtag attggaatcc
      601 tgcgctgtat gaaaaagtga ttacggccac caactatcag gaagccgcac aagcattgca
      661 agatgcaggt tatgcgacag accccggtta tgcgcaaaaa atcatccagg tcatcgagac
      721 gtatcaattg aatcagtatg accggagata gcaaaggaga agtggctgat ggcagaacaa
      781 tttacgccag aaatcatgac ggaattgaga aaagatatcg taagagtacc ggaaatcatt
      841 ttagaagcaa gcgggatcaa gatatttgga aaattgatcc gttcgatcat ctttactacg
      901 gatattgcga tcatacgtaa tacaaatgcg aatgcagtaa ttgctgtgta tccttttaca
      961 cctcatccag cgattactaa aagcattatt gaggcagctg acattccagt tttctccggt
     1021 gtaggtggtg gtttgacaca aggtgctcgt tcttcctaca tgagcatgtt cgcagaagcg
     1081 caaggttcgt taggggtcgt tttgaacggt cctacgcctt tagagacagt aaaagaagtt
     1141 tgtcgagtag ttgatattcc agttatcagt acggtaacgt cgaaatatac agaaatagat
     1201 gaaaagttag cagctggtat ttcgattatc aatatcagtg caggtaaaga aactgcagaa
     1261 gcagtacgcc attttcgtaa aaaatatcct gaacttccta tcattgcgac aggtggtcca
     1321 acggaagaaa gtattgctga gaccattcaa gcaggagcaa atgccatcac atatacgcca
     1381 ccaagtagcg gagagctttt tagtcgtaaa atggaaaaat atcgggaaac tgagaaaaag
     1441 aatcatgaat aacaggtgag aatcaaaaga cgaggaagag cagatagtgg ctcttcctcg
     1501 tcttttgtac tccagtacta tttgttcaga attcaaaaaa aagagaaatt tttttgtttt
     1561 tttacctgta atgtacataa gaagagttaa aaccttttgt tattattagc attctaacaa
     1621 aaaaatgtct tgatttattg aagcgctttc tatagaatga aaaacgaata aaaactaaaa
     1681 aaggcacccg acaatttagt agcagctaaa ttgtcggggc ttgaaaagtc agcgggaact
     1741 gaccaatcaa gttgccatag tcaatgcttg tgcatcgacg tgtatattgt acctaatttc
     1801 atacagaatt actagttatt tttctgtttt ttagggaaga gcgcacacga agaagcatgc
     1861 ttatttagct atgtcaagga tattgggcag ttgtctgata tccttttttc tttgcgtaat
     1921 tatcctccca tcattgaagc aagaagtgtt tttagcgatg aaagaagaat cagcactatc
     1981 taatttttct tgaaagtatt gagttgttca tcctaggcag aacgataagg caagtcttgg
     2041 tttttattcc ccttgaaaaa gatcgtttaa taaatatgtt ctgtataaaa atacagtata
     2101 ttgaaaaata acaatggaag tagaggtgca gagaatgaag aaaagaatga gaatcatctt
     2161 agctttaagt gttatgttat tcttcttgac ggcttgtggg caagaagatg acaaaaaaat
     2221 cacgctgaac atggcttggt ggggttcgca agtccgacac gatgcaacag taaaagtaat
     2281 cgagctttat gaagaaaaaa atccccatgt gacgatcgat tatgaatttt atgattatga
     2341 aggatattac acaaaattag gtacactagc agcatcagat gatatctggg acattttcca
     2401 actgaacaat caatttcctc aatacatcaa tcagattgaa ccattagatg aatatgtgga
     2461 gcaagggata attgatattt ctaatgcaga tgaatcctat ttagcaacga cgacttacga
     2521 cgggcaatta gttggactgt ctaacggggt gaattcgttt gcgattgctt acaataaaaa
     2581 tatatttgaa caattaaata tccctgaacc agcttcaaat tggacatggg atgaatttga
     2641 acaaatcagc tatcaaatca ctgaagaatt aggaatctat gctatttcaa gattcgaaga
     2701 ttttatagct ggctgtatta ttcaaatacc acaagttgag aaaggattga atttttataa
     2761 tcgggaagat ctatctaaca gcttgggatt tgaaaatcca gactatctaa cagatttttt
     2821 ccagctgcgt aaaaatctgg tagacaaagg ggcccatcca gaaccaggtg gtggagctgc
     2881 aagtacgacg gatctgtctt ttcaggcggt tagggataga gaagcggcaa tggtgtttct
     2941 atcaagtaac cagttgacag aaatattgtc aggggctcct gaagatatgg agattcagtt
     3001 gattaatcca cctcgcagaa aagcagatgg agaatctggt atccctttac gttcttcgca
     3061 aatgctgagt atggcaaaaa gctcagccca taaagaagaa gctgctaagt ttattgattt
     3121 ctttcaaaac agtgaagaag ccaatgaaat tttaaaagga gaacgtggtg taccaatcat
     3181 gtcgaatatt cgagaacagc tcgctcgtgc agatgatgca actttgcaag cgacatttga
     3241 ttatttagaa atgatttccg atatggacaa cggcgaagtg aatgtctttg atagtgcttt
     3301 gaatccggaa attcaagacc aatacaatct tttagttgaa aaagtcattt atgaagaagt
     3361 aacagcagaa gaagcagcag agagtcttta tgagtttgcg tcaaacattc taggatagca
     3421 gcaggaggaa ggcatgaaaa aagaaaaact aaaacggttc ttggatcaag acaatgtggt
     3481 cggttacgta tttgcttctc cattcatcct aggatttcta ttgataacag tgataccgat
     3541 gatcttgtca ctttattatt cgttcaccga ttatcgtttg ggaaatccag tctcatggat
     3601 cggcttggat aattatataa agatgctgag tgatgcccgt tttttgaact ccatccgagt
     3661 cacttttctc tatgtatttg catctgtacc tgtgaaactg atctttggtc tattgattgc
     3721 ttatctttta acgaggaata tcaaaggaac gactttcttt cgttctttgt attatgtgcc
     3781 tagtttgatc ggtggcagta tttctatcgc tctagtttgg cgggagttgt tttctaaaaa
     3841 cgggttagtg aatattttcc tggaacgatt aggcgcacct gcgctttcct ggtttagcga
     3901 tcaaaaactg gtcatgatcc ctcttgtttt gatgtctgct tggcaattcg gttcggcaat
     3961 gatcatcttt gctgcaggta tcaaagagat cccaggctcg tattatgaag cggcagagat
     4021 cgatggagcg aacaaatggc agacgtttta caagatcacg ttacctcttc tttcgccgat
     4081 cattttgtat aacttggtta tgcagacaat cgcggcgttt atgactttta cgcaggcatt
     4141 tatcattacc caaggtggtc cgaatgatgc cacgaacttt tatgctcttt atgtgtataa
     4201 ccaagctttt tcttttaatt ctatggggta tgctagtgct atttcgtgga tcatgctgtt
     4261 gattatggga gctgtcacct ttctgatttt caaatcatca aaaaaatggg tcgttgtgtg
     4321 aatgagagga ggaagaaatg aataatacag taacgaaatg gtttcaatac ggttttattt
     4381 tgctattagg tctagtcatg atttatccgg tcttatggat gatcgcaggt tcttttaaaa
     4441 cggatcaaga gattatgagt ggttcgctga atttgattcc agagactttt caatgggaaa
     4501 attacgcaaa aggatgggcg gggtttgctg gaatttcatt ttttactttc tttaggaatt
     4561 cgtttgtcat ctctgcagtt tcgacgatag ggacagttct gagctcgact tgtattgctt
     4621 acgcattatc gagaatcgat ttcaaaggaa aaagattctg gttcgtcgtc atgttagtaa
     4681 cgatgatgat tcctgcacaa gtgattttga ttccgcagtt tattatttac aatcggctga
     4741 atttggttgg gacgtatgtg ccgctgattt taccacattt ttttggacaa gcatttttca
     4801 tttaccagat catgcaattc atggtcagca tccccaaaga gttagatgaa tcagcgatta
     4861 ttgacggatg cagcaagtat agtgtcttta ccaaaattat ttttcctttg ctgaaacctt
     4921 cgatcatcac gacgatcatc atccagtttt attggaaatg ggacgacttc atgggaccat
     4981 tgatttattt aaacaagccg agatcttaca cggtatcgat tgccatcaaa ttatttgcag
     5041 acgctggatc tagtacgact tacgcgagta tgtttgcgat gtctacgttg tcgctgctac
     5101 cggtttttct gattttctta ttcttcaata aatatcttgt ccaggggatc agtacaagcg
     5161 gtctgaaggg ctgaatatga aaaagccaat agaagagatg tttctttaca agctcaacta
     5221 ctatgtctgt cggatttttt taaccagtct tatcagtaat gctcttttcg ctttagttct
     5281 aggatgtttg ttcactttag gattagtgct gtctgctcgt gttgtctatc tgatttattt
     5341 tttgatgatg tatccttctt attatctatc tgtccattac ttggcagaaa aaaaggtatg
     5401 ttggaggaaa ggaaaaatca aacggatgtg tttcatcatt acgttgtttc agctgtttgt
     5461 cattattttt gcagttgatt ggtatttatt agtgggaaag attggagctt ggtatctgat
     5521 tccttttcta ttgtgtctgt tgctttttgt atcccatagt ttttttagta cgctaattga
     5581 tatctgcaaa gaagataaag cagatagtct tgtttatttg atcaaaagga atatctggct
     5641 gttgtcaaaa aacgtcaagg ggtttctgtg gtatttatgc cttttgtatg gactgttctg
     5701 gtgtatacag catgattttt tactcactgc attactgtat ccagggctgc catttgtcac
     5761 agtattttgc ttaaaagaaa aggagaatga ctttaaatga agaaaaaata tgtacaagtc
     5821 ggacttggtg ggagagcgcg ttttttttat gaggcaattg cctctacgta tcatcaaact
     5881 tgtgaactag tcggtttttg tgatgtatct caaacaagga tggattatgc aaatcggata
     5941 cttgtgaaca agtatggaca gaaaaaagta aagacatgta tgccatcaca atttgagcaa
     6001 ctactggatg acgtacggcc ggctgaagtc attattacgt cagtagatcg aacgcatcat
     6061 gattatatca ttcgtgcaat gaaaaaagga tatgatgtta tttgcgaaaa acctctgact
     6121 atcgatgagg aaaaagctca agaaataata acggtccaaa aacagacggg aagaagcctg
     6181 cgagtcactt ttaattatcg ttatgcaccg tatcacacga agataaagga actgatagaa
     6241 agtgatgtga ttggtgatgt gttttctatc cacttcgaat ggctgttaaa tacgcaacac
     6301 ggggcagatt atttcagaag atggcatcga aataagcaca atagcggcgg gcttcttgtt
     6361 cataaatcta cccatcattt tgatctagtg aatttttggt tgaacagccg tccccaaaag
     6421 gtatttgcat tcggtggatt aaacttttat gggatgcata atgcggagaa acgcggagta
     6481 gaaatatttt acagtcgatg ctatggaagc gaactagcta aagctgatcc atttgctata
     6541 gacctgtcag ccaatgagga attgcgagaa atgtatttag aagcagaaaa agacagtgga
     6601 tatattcgtg accgcagtgt attttccgat gatatctcta ttgaagatac actaggcgta
     6661 acggtcaaat atgaaactgg tgcgattttg acgtattcgc taaacgcgta tatgccgtgg
     6721 gaaggattca atgtttcttt caatggcagc aaaggacgga tcgaaatgaa agtgatcgaa
     6781 aagtcttaca tcaatgcagg tggagataaa aaggatgaag gagcagccgt tcttcagtcc
     6841 atccatgtct atccgatgtt cggtgagcca tatgaggtaa tcgtcgaaca agaagcgggc
     6901 ggacatggcg gaggagatcc gcgtttatta gatgatctat ttggtgaaga aaaagaagaa
     6961 gatccgtgga atcgggcagc aactcacgta gatggtattt tgtcgatttt gacaggaatc
     7021 gcagcaaatc attctattgc ttcaggaaaa gcagtagtga ttgacgagct tgtcagctta
     7081 aaagaataag aatagattta cggaaattac ttcccgtagt ttataaaatc ttgccttatt
     7141 gccgataggc tgtcttttga acactattta ttcggaaaaa ggggctatga catgcttttt
     7201 gtcatgcctt ctttttatgt gggctgcttt tttagatagg agaaaataaa aataactatt
     7261 taaacccgaa caaaaaggaa taatgcatta aaaatcatta gattttacaa aaaaataagt
     7321 aaaccacgtt gtcagtttca gaaagtagtg ttagactgtt ttcaatattt gaaacaacat
     7381 tttttattca aatatggcaa actgccttca cgtgcaaaac agttgcaaga aaatgaattt
     7441 acactattat tagttttaca tacctactta tatatcgtca taatatggat gacagtttct
     7501 agccagtacc gtaaatgctg gactataagt aaaagattgg ctatttcacc cttcttttct
     7561 tatgataaag tggagggtgt ttttctttaa cagaaacata gtaggttttt gcacaaaagc
     7621 ggttatttcg gtattaataa atgataaagg agcaaataaa gtgaaagaat tagttgaacg
     7681 tattcaaaaa gacggacgta tattaggcga gggcgtatta aaagtcgaca gcttcgtcac
     7741 tcaccaagta gaccctgtat tgatggaaca aatgggaaaa cggtttgcag aagtatttaa
     7801 agaacaagga atcacgaaag ttgtgaccat tgaagcttct gggatcgcac cagctttatt
     7861 tgctgcaaga gaattagatg ttccaatgat cttcgctaga aaagcaaaaa gtttgacgat
     7921 ggatgaagaa ttattgacag cttctgttta ttcttttacg aaacaagtga caagtacgat
     7981 ttctatttct cgtaaatttt tatcttcaga agacaaagta ttgattatcg acgatttctt
     8041 ggcaaacgga caagcggcaa aaggattgat cgaattatgc cagcaagctg gagcgcaagt
     8101 agaaggaatc gggatcgtca tcgaaaagtc tttccaagat ggacgtcagt tacttgaaga
     8161 aatgggattg agagtcgttt ctcttgcgcg tattgcttcc ttgacgaatg gacaagttga
     8221 gtttcttgag gaggacgcat aatcgtggaa aaagaaacac aaaatggaaa agctgcggtt
     8281 ttagggctgc agcatctgct agcgatgtat gcaggagcag tagcggtgcc tcttttgatc
     8341 ggtacaggac taggctttaa tcaagaacag atgacttatt tgatttcaat tgatatcttt
     8401 atgtgtggat tagcgacatt attgcaattg acagttaacc gatttttcgg aattggcttg
     8461 cctgtcgttt taggatgtgc gatccaagca gtagctccac tgatcttgat cggaaccaat
     8521 gaaggagtag gagctatcta cggatctatt atcgcttctg ggatctttgt tgtgttagtt
     8581 tctggtgtct tttctaaaat taaaaaacta ttccctccta tagtaacagg aacggttatc
     8641 accgttattg gtttgacttt gattcctgta gcaatcgaga aaatgggtgg aggtaatgca
     8701 acagcagctg attttggtga caaaacaaat ctgctacttg catttgtgac gattctccta
     8761 attgtaggcg tgcaaatgct ggcaaaaggg ttcattcgtt caatcgcggt attgattggt
     8821 ttagtcggag gaagcatctt agctgctttc atgggaatga ttgatctgag cgcgattgga
     8881 gatgcgccgt tgttccatgt tccacaaccc ttttattttg gtaaaccaac tttcgacgta
     8941 tggtcgattt tactaatgat catcatttcc atggtcagca tggtagaatc aacaggtgta
     9001 tattttgcac ttggtgacat tactgggaaa aatgttggag aagaagaatt gaaaaaggga
     9061 tatcgtgcag aaggacttgc cgttatctta ggcggtattt tcaatacatt cccttatact
     9121 ggcttttctc aaaatgtcgg attggttcaa ctttcgggaa tcaaaacaag aaaacccatc
     9181 tatttctctg cattcttctt gattgtttta ggattattcc ctaaaatcgg tgcgttagca
     9241 caaatcatcc ctgagccggt acttggtgga ggaatgttag tcatgttcgg gatggtggca
     9301 gtccaaggaa tccgtatgct gttggaagtt gatttcagca atgataaaaa tctattgatt
     9361 gttgctgttt ctgttggatt tgggctgggt ttcaatgtta cgccgacatt attcagtcaa
     9421 atgccgcaaa cattgcaaat gttcactgga aatggcatcg taatgagtag cattacggct
     9481 atcatattga atttgatctt taatggattg aaaaaagatg aagaaatatt ggaagaagcc
     9541 ttcaagaagt aatagagcaa aagaaaggtt gtgaaatcag cgttttgacc tgattgttag
     9601 agaaacaaac ggaaaaatag ttcctcgtat tttgccgttt gtttgagcta ataccgaggg
     9661 tcagctgatt gaacaccgtt gaaggaagag gtcgtgagcc tgccgttcat ctccgagtca
     9721 caaggaacaa tttcaaaaaa aagcttctca tgtttgttga atattgttcc ttgtgaccga
     9781 ggagttggca ggagaacacc gtttatccat aaaaaggaat gtgacctaac ttttgtcaca
     9841 ttcctttttt ttatctttaa taaacacgaa caataaaatg tttttctttg ttaacattcg
     9901 aaatttcttt gacatcaaaa aaagtgattg ttaaaatatg aatgtagaaa aactgggagg
     9961 aaaaacaatg acagcaaaaa tctctgtgat tatggggagt acttcagatt gggaaacaat
    10021 gaaagaagca tgtcaaatat tggatgaatt cggtgtgtct tatgaaaaaa aggtcgtttc
    10081 tgctcatcga accccagatt tgatgtttga atatgcacaa aacgctcgaa aaaaaggaat
    10141 caaaatcatt attgcaggag cagggggagc cgcgcatctg ccgggtatgg tcgcatcaaa
    10201 aacaactttg ccggttatcg gtgtaccggt ccaatcacga acattaaatg gtttggattc
    10261 gcttctttcg attgtccaaa tgcctggagg agtacctgta gctactacag caatcggaaa
    10321 agcgggagca attaatgctg gactacttgc gatacagatg ctttccatgt atgatttaga
    10381 attggaggct aaattggctg aaagacgttc aatgttggct aaaactgtta tagaaagtag
    10441 tgatcaactt ggttaaacct ttgttgcctg gttcaacgat cgggatcgtc ggtggcggtc
    10501 aattaggtcg tatgatggcg attagtgcaa aagaaatggg ctttaaagta ggtgtgctgg
    10561 atcctgtgag cgattgtcca gccgcacaag ttgcagattg gcacattgaa agtgtctatg
    10621 atgacacctt tgcgctgaaa gagcttgcca gaagaacaga tgtcattacc tatgaattcg
    10681 aaaacgtcag tgtagaggcg ttaaatgcga ttttacccat gtcatttatt cctcaaggga
    10741 cagacttgct tgcgattacg caagaccgcc tgttagaaaa atcttttttg gaaacgaata
    10801 acatcgtgat tgctccttat gcaacgatcg tcagtccgac ggatatccaa gatgcgattg
    10861 acgggatcgg ctatccttgt gttttgaaaa ccactcgagg cggctatgat ggcaaggggc
    10921 agtatgtttt gaaaagtcgg gcggatctag ctcctgccat ggacctttta agagaaggaa
    10981 cctgtgtttt agaagcatgg atcccgtttg aaaaagagat ttcgatcatg gtggcaggaa
    11041 atggtcaagg agatttcacg acttttccgg tcgtggaaaa catccaccac aacaatattc
    11101 ttcatgaaac gattgctcca gccgcgattg atcaggatgt gatcgaggag gctgagcgta
    11161 ttgcccgtgt gattgcagaa gcagtgagcc tttcaggtgt tttaggggta gaaatgtttt
    11221 taacaaaaac aggcgggcta tatgtgaatg aattagctcc tcgtcctcac aattctggac
    11281 attattctat tgaagcttgt tctatgagtc agttcgatgc ccatattcga ggtatttgtg
    11341 gttggccatt aggagaaaaa gtacgtttga taagcaatgc agtaatggtc aatgttttgg
    11401 gagaacaact gctggaaagc tatcgcttga ttgagaataa gccagactgg caattccatt
    11461 tctatggaaa aaaacaagca aaaaaaggtc gcaagatggg acatatcact atcttgacag
    11521 aagacatcca gcaaacgctg gaagaaatca aacaaacgaa tatttgggat tagaggagaa
    11581 aaaacatgtt agaacgttat acacgccctg aaatggggaa tatctggacg gaagagaaca
    11641 aataccgtgc gtggctcgaa gtcgagattt tagcagatga agcttgggca gaacttggtg
    11701 aaatcccgca agaagatgtg aaaaaaattc gtgagcatgc atcatttgat atcgatcgga
    11761 tcttggaaat cgaaaaatta acacgccatg atgtcgttgc gtttacacga gcggtttctg
    11821 aaacattggg agaagaaaga aaatgggtcc attatggtct aacaagtacg gatgtcgtag
    11881 atactgctta tggctatctc atcaagcaag caaatgatat tttacgggaa gacttgaaac
    11941 gttttacgga aatcattggc gaaaaagcaa aagaacacaa atatacagtg atgatgggtc
    12001 ggacacacgg tgtccatgct gaaccaacta cttttggatt gaaacttgcc acatggtatt
    12061 cagaaatgaa acggaacatc gaacgatttg aacatgcggc aaaaggagta gaagcaggaa
    12121 aaatctcagg agcagtggga acgttcgcaa atatcccgcc atttattgaa gagtatgtat
    12181 gtaaacaatt ggggatccgt ccacaagaaa tctctactca agttctccct cgggatctac
    12241 atgctgaata tttctcagcg atggcgctta ttgctacaag tattgaacgt tttgcaacag
    12301 agatacgcgg attgcaaaaa tccgaaacac gtgaagtaga agaatttttt gcgaaaggtc
    12361 aaaaaggttc ttctgctatg ccgcacaaaa ggaatccgat cggttcagaa aatatggctg
    12421 gtctagccag agtggtgcgc ggacacatgg tcactgccta tgaaaatgtc acattatggc
    12481 atgaaagaga tatttctcat tcttcagcag aacgaatcat cttgcctgat accacgattt
    12541 tattgaatta catgttaaat cgttttggca atatcgttaa aaatctaacg gtctttcctg
    12601 aaaacatgaa gcggaatatg tctgcaacat ttggtttgat ttatagccag cgcgttatgc
    12661 tgaaattgat cgataaggga atgacaagag aagaagctta tgatcttgtt cagccaaaaa
    12721 ctgcgtacgc ttgggacaac caagtgcagt tccgcccgct gttagaagaa gatccaacca
    12781 tcacatcggt attatctaaa gaagaaatag acgatgcctt tgattatcat catcatttga
    12841 aaaacgttga cgtgatattc aaacgagtag gtttggaata aagaagagtg agttgcaaaa
    12901 ttcaattaaa atagaatagc aaaaataatc gagtagaggg gaaagccaac tgaccttccg
    12961 cccctctcac accaccgtac gtacggaccc gtatacggcg gttcaataag ttgagtgtac
    13021 gaactggtac ttcaaggaga tgtcctcata tccttggcgg gccagttctt tgtctgttaa
    13081 tgatcgatga agaatatgac ttttggagac tgaccaaata ccttttcttg tatttgacca
    13141 ttcatacgct ttcgctttat cgatccctaa acgaatcaga ttttttcttt tggttcttgg
    13201 attcttccat tgtttccaaa tatattgtct gattcttcgc ttcaaccaac cgtttaactc
    13261 cctcatgaat tctttcatct caccaattcc atagtagttg atccaaccag tcattagttg
    13321 cttgatttct tttaaaataa gttggatact tcttccacgg tttcttttag tgagttgttt
    13381 gagcttgctt ttcacacgct gttttgcttt tgcgtgaggg cgaattttaa tacctttctt
    13441 tgttggcaga atgcagaatc caagaaactt tcgtttcaat ggacttccta ccgcactctt
    13501 ttctcgattt accgtcaact ttagttgttc ttcgagaaat tgaatggatt tcttcatcac
    13561 acgctgtcct gctcgtttgc ttctaacata gatattgcag tcatcggcgt atcgaacaaa
    13621 tttatgtcct agattttcta gcacttgatc aaattcgttc aggtagatgt tacttaacag
    13681 gggagagaga ttgcctcctt ggggcgttcc ttcttcagtt gttgtgatga gtccattttc
    13741 cattacacca ctcattaagt attgacggat cagttttagg acttgtttgt cctttacttt
    13801 ccgctcaatg aaatacatca gcttatcgtg atttacggtg tcaaagtagg ctttcatatc
    13861 aatgtccacc acatatcggt aaccttgttc ataataagat tttgctttct tcactgcgtc
    13921 atgtgcgctg cgatttgggc gaaacccata actattatct gagaattctg gttcaaatag
    13981 cggttgtaac acttgattga ttgcctgttg aagcatacga tcaataaccg tgggaattcc
    14041 tagttttctt acaccgccgt ttggtttcgg tatttctact cgcaaaacag gctgaggttt
    14101 atagttccct tggcgtaaat tggatagtaa ttcctcgcgg tgctctttca agtaaggaag
    14161 cagttgatcg acggtcatcc catctgcacc agccgcccct ttatttctga ctacttgaag
    14221 atacgcttga ttgagattat tccgatctag aattctccca aacagatttt ctacaccatc
    14281 ttgtttcctc tgttcaccgc aagaagtgct gcgcacttca tcataccctt cgtgttccac
    14341 actagctctt tgatgatagc caagttttct tgttttctgc ggtcgtcgca tcttctcaca
    14401 cctcctaaat tattcaagat tcttattgtt cagtccttcg taacggtcag tgtcgttact
    14461 actatgacct cggctgactt ctcatgattc accatcatgt cgccatgatc gttgtgctgg
    14521 tttgttttca ttcaacgccc acaacaccct catgagatct cccccggtaa gagtgaaaac
    14581 tttcctccca tgtaactgct agatttactg tataggtttc gggcagtatt ggactttgtc
    14641 ttgtgatgaa gacttatccg acctaattca gccttgtatc tagtttctgt tcgtcagttc
    14701 aggagtttgc gtccgacttc cttcagccac aacctcacga ttgccgcctt gtctttcgct
    14761 aacagttcct actgccaagc ctgtagtgga ctttcaccac caagttttca cccatgcagg
    14821 gcgcacacca aaaaagaagc agatccgtta gcggttctgc ttcttttagt ttattatatt
    14881 ttcttatcaa taataggaac ctgattttaa aagcggctac ctctttaccg tgcctcttta
    14941 catacaaaaa atagaggaat taagcgtaaa tggcttggct attataacta aaatgctggt
    15001 tatggagatg ttgcagcaag ttttcgagaa aagcgtattc catctcttgt ccattgaata
    15061 agatctggat acgataatga tttttgaaac gcttctgttc atgaatctga ctgattgaaa
    15121 gttcgccttt gtgttctgtc tgataaagaa aagtcctttt acggcattca gcacagcgct
    15181 tggttgctcg gcggattttt cggaatgtat aaaaactagt atctttattg cataaataaa
    15241 cgtctttgat cgacagcaaa tcattatata gacaggaacg ttcttcagct gttaattctg
    15301 ataaaaaaag ctgaccttgt tttattgcat cttccatcgg caagtgcctc ctcgaaccca
    15361 ttatccaaat ttttagttaa ttctaatata ccatcattaa gtataaatgt aaacgcttgt
    15421 tgatggtata agattttcgt ttctgataaa acacgaacat taaaagaatc taatgcctta
    15481 aatgtacata tttttgttga tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac
    15541 gaacaaaaaa cgatgtattt gcttttaatg taaataaaaa ggagagacgt acatggagaa
    15601 gaaagaactt ttgtatgaag gaaaagccaa aaaaatatat cttacttctg atgagaactt
    15661 gatttggata gaatatttag atcaagcaac tgcgttgaat ggcgcgagga aagaccagat
    15721 aagtggaaaa ggagaattga ataatcaaat cacaagtgca attttccgtt atctgactca
    15781 aagaggaata aaaaatcatt ttattgaaca attgtctaaa accgaacaat tagcaaaaaa
    15841 agtacaaatt atccctctgg aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa
    15901 acgtctaggt gttcccgaag gtaaagaatt catcaaacca atcattgaat tctactataa
    15961 ggatgaccag ttggatgatc catttatcaa tgaagagcat atctatttct tacaacttgc
    16021 aaagccggaa gaaatcagtg agctgaaagc ccaagcgttg gctattaatg aaattttgac
    16081 cgaattattt gctaaggtcg gtttggtttt agtagatttc aagctagaat ttggccggtc
    16141 gaatggacaa attttgcttg cggatgaagt aacaccggat acttgccgat tatgggatga
    16201 acgtacgaat gaacatatgg acaaagacgt ttaccgcaga gatctaggtg atttgatccc
    16261 agtctatcaa gaagtatata accgtctatc ggaactaact gaagaaaagg agcgagtatg
    16321 atgtatttcg taaaagtata tgtaacctat aaagattcag tgttagatcc tcaaggggag
    16381 gcagtaaaaa aagcagtcca tcgtatggga tatgaaacaa tcgaagatat ccgaatagga
    16441 aaatactttg agatacaact tgctgatgca gaagaaccaa tcgatacagt gattgaaacg
    16501 atttgtgaca agctgcttgc aaatgtaaat atggaaacat accgatatga aatcaaaaaa
    16561 atggaggaag tataacatga aatttgcggt catcgttttt ccaggttcaa attgtgacgc
    16621 agatatgtta tgggcagttc gcgagatcat gggcgctgat gccgaatttg tacggcatga
    16681 tgagtcctct ttagaaggat ttgatggggt tttactgccg ggaggattct cctatggtga
    16741 ctatttgaga tgcggtgcga ttgctcggtt ttctacaatc atggaagaag tgattcgttt
    16801 tgctgaagaa gggaaaccag tattcggtac atgcaatgga ttccagatat tgacagaagc
    16861 aggacttttg ccaggtgtac tgagacgaaa tgagtctttg cattttattt gcaaaactgt
    16921 accattgaaa gtcaacccca agacggcctt tacttcagag tatgaagccg acgaaataat
    16981 ctatcttcca gttgctcatg gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga
    17041 actaaaaaaa aataatcaga ttgcctttac ttataatgac aacatcaacg gcagcgtgga
    17101 aaacattgca gggattacga acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga
    17161 acgtgcagta gaagacttgc taggatcaga agatgggaaa cggttctttg cttccatttt
    17221 aaaaaacttc ggaaaggtga ccgtatagat gaaaacattg aaagaaccga cagctgaaga
    17281 gatcaagaaa aaaagaatct atgccgaatg gggattgact gatgaagaat atcgaatgat
    17341 cgaagaagat atcttaggaa gaatgcctaa ttatacggaa actggactgt tttctgttat
    17401 gtggagcgaa cattgttcct ataaaaattc taaaccggtt ctacgcaaat ttcctactag
    17461 tggacctcat gtgttgcaag gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg
    17521 actagcagtt gtcttcaaag cagaaagtca caatcatcct tcagccgtgg agccttatga
    17581 aggagctgcg acaggagttg gcggaatcat cagagatatt ttcagcatgg gggctcgtcc
    17641 aattgcgttg ctagattcat tgaggttcgg cgaattagat aatccaagaa cgaaatattt
    17701 actagaagaa gtagttgcag gtatcggcgg atacggcaat tgtatcggca taccgactgt
    17761 aggcggcgaa attgcatttg atccttgtta tgaaggaaac ccattagtca atgcaatgtg
    17821 cgtaggtttg attgaacaca aacatatcca gaaagggcaa gccgctggcg tcggtaattc
    17881 gatcatgtat gtaggtgcaa aaacaggtcg cgacggtatc cacggagcaa cttttgcctc
    17941 agaagaattt aatcaagaag aagaacagca acgttctgct gtacaagtag gcgatccttt
    18001 tatggaaaag ctgctgcttg aagcgtgttt ggaattaatc ttgcatcatt cggattctct
    18061 aataggtata caggatatgg gagcagcagg acttgtttca tctagtgcgg aaatggcttc
    18121 aaaagctggc tcaggactta tattgacatt agatgaagtc ccacaaagag aaactgggat
    18181 gacaccttat gaaatgatgt tatcggaatc acaagaacgg atgttgattt gtgttaaatc
    18241 aggagaggaa gaaaaaatcc aagaactatt tcagaagtat gatctagatg cagtcactat
    18301 cggtaaagta acagacgacg ggcaatatcg gctatatcat caaggagaag aagtagcaaa
    18361 tctgccagtc gatgcattag ctgaagatgc accagtttat cataaagaaa tgaaagaacc
    18421 agctcgaatc gtagaatttc agcagttagc tccttatcag ccagttatcg aagaacctgg
    18481 acaagtattg cttgatctgc tgcagcaacc gacgattgct tcaaaacgtt caatctacga
    18541 aacttatgat tcacaagtac aaaccaatac agttgtccgt ccaggaagcg atgctgctgt
    18601 gatgagggtt agagggacga ataaagcgtt agctatgacc acagactgca acgcgcgcta
    18661 tctgtatttg gatccaaaaa tcggaggaca aatagcagtg gcagaagctg ctagaaatat
    18721 tattgctagc ggtggtaagc ccttggcgat caccgactgc ctgaactatg gttctcctga
    18781 taaaccagaa gttttttggg aattatcgac ttcagctgac ggaattgctg ctgcgtgtga
    18841 aacgttagga actcctgtta tttcaggaaa tgtctcttta tataatgaaa cggatggtca
    18901 agcaatctat ccaacaccaa tgattggtat ggtcggattg attgaggatc ataaacatat
    18961 cactactcag gaattcaaaa aatcagggga tctaatctat atattaggta agacgtttgc
    19021 agattttgat ggcagtgaat tacaaaagat gcagcttgga agaatcgaag gtgtgatccg
    19081 aaactttgat ctatccattg aaaagagaaa ccaagagtta gtattgacag ccatccaaaa
    19141 cggattgatt gaaagtgctc atgactgttc agaaggtggc ttggcaattg cactggcaga
    19201 atcagctttc aaacatcagc tagggatatc tgtacagttt gaattgtcct ctgctcaatt
    19261 atttgcagaa actcaatcaa gatttgtgtt gacagtcgca ccggaaaata agacacgatt
    19321 cgaagaaatg atgggagatg ctgctgtttt agcaggtaag gtgacggatg aagcgataat
    19381 cgaaatttca gcaactgacg gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg
    19441 ggaggatgca atcgtatgtc ttatgaagtg aagagtttaa atgaagaatg cgggatcttc
    19501 ggtgtttggg gtcatccaga cgccgctagt gtggcatact tcgggctcca tagtcttcag
    19561 catcgaggac aagaaggtgc aggtatcgtg tccaatgatc gaggaaagct taaggggcat
    19621 cgagatttgg ggctgctttc tgaggtattc aaagatcaaa gaaaattgca gcagttaaca
    19681 ggagaagctg caatcggaca tgtacgttat gcaacagcag gcaatggaag tgtggacaat
    19741 attcagcctt ttctattcaa atttcatgat cagtcgatcg gcttagcgca taatggtaac
    19801 ttgatcaatg ctcgttccct tagaaaagag ttagaggaaa atggggcgat cttccattct
    19861 aactccgata cggagatatt gatgcatttg atccgacgaa gtgaagaaac agatttttta
    19921 aataaattaa aaagtgcttt gaatgaagtc aaaggtggct ttgcctattt attaatgaca
    19981 gaaacggcaa tgattgccgc tttggaccca aatggttttc gtccattagc aatcggacaa
    20041 atgaaaaacg gagcatacgt gattgcctcg gaaacttgtg cattagaagt tattggggca
    20101 cgttttataa gagatgtcca gcctggtgag atagtgatca tcaatgacga aggaatccag
    20161 attgattgct ttacgaagga cacgcagctt tctatttgtt cgatggaata tatctatttt
    20221 gccagaccag attcaaacat cgcagggatc aatgtgcata ctgcacggaa aaatatggga
    20281 cggaatttag cgaaagaatc gccagtaaaa gcagatatgg tcgttggcgt acctaattct
    20341 tctttgtcag ccgcaagcgg ttatgcggaa gctagtggaa tcccttatga aatcggatta
    20401 gtaaaaaatc aatacattgc ccgaacattt atccagccaa cgcctgagct aagagagcaa
    20461 ggtgttcgaa tgaaattatc tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg
    20521 gtcgatgatt caattgttcg gggtacgaca agcagacgga tcgtccatct tttgaaagaa
    20581 gcaggggcaa aagaagtcca tgtgaggatt gcttctcctc ctttgaaata tccatgtttt
    20641 tacgggattg atattcaaac gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa
    20701 atcaaagaat gtatcggtgc agattcttta ggttttttaa gtgaagaggg tctgatcgaa
    20761 tcgatcggtc tggaaaaaga tgctccgtat tcaggattgt gtatggctta tttcaacgga
    20821 gattatccga cgccgttata tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa
    20881 gtatcgttct tttaaaagca gaccaagaag ggagtatatg aattcaatgg caaatgcata
    20941 cgcaaaagct ggcgtagatg tcgaagctgg atatgaagta gtagaacgga tcaagaagca
    21001 tgtcaaaaaa acggaacgtt taggtatgat gggcgctata ggaggattcg gcggttgttt
    21061 tgatttatcg gtctttcaat taaaagagcc agtccttgtc tcggggaccg atggcgtggg
    21121 gacgaaatta gcagtggcaa tcgaagcaga caaacacgat acaatcggga tcgactgcgt
    21181 agcgatgtgt gtcaatgata tcttagcaca aggcgcagag ccgttatatt tcttagacta
    21241 cttagcaacg gggaaaaatg atccgttacg tttggaaaat gtagtaaaag gtgtagcaga
    21301 tggatgtgtt caaggaaata tggcgctgat tggcggagaa acagctgaaa tgcctggtat
    21361 gtactcaggt aatgattatg atctagccgg atttgctgta gggatagctg aaaaatctca
    21421 attattgaca ggagaaacga tcaacccagg agatgtgttg cttggaatag catctagcgg
    21481 gattcattca aatgggtact ctcttgttcg caaaattttc tttgagcagc agcagttttc
    21541 tttatcatca aaactgccag aattgaaaaa aacacttggc gaagaattgc ttgagccaac
    21601 gaaaatctat gtttcttcct tgcttccgct gatcaaagaa aagaaaataa aaggagctgc
    21661 acatatcact ggtggagggt ttgtagaaaa tatcccgcga atgttaccag aatctatggc
    21721 tgcacaaatc aaacttggct catggccgat tcttccaata ttcggctgtt tacaaaaata
    21781 tggtgaaatt cctgagatgg agatgtatga aatcttcaat atgggcatcg gtatggtcct
    21841 ggcagtccaa gaatcgaaag taaaagaaat agtacacgaa attgagcaag ctggcgaaaa
    21901 agtttatgaa atcgggaaag tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga
    21961 agaacgatga gaatcgctgt ttttgcttct ggaaacggaa gcaactttca agcgctagcc
    22021 gattatctat cgaaaaaagg attggaatct tcgattgact ggttgttttg tgatcagcct
    22081 gaagcatatg ttttgaaacg tgcaactgcg ttgagtgtac cggcagattg tttttcacct
    22141 aaagaatttg attcaaaaaa agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa
    22201 aagatcgatt tgatcgtatt agctggatat atgcgaatca ttggaccggt attgctgaaa
    22261 aattatgata aaagaatcat caatatccat ccgtctcttt tacctgcctt ccctggtttg
    22321 catggcatac gtgatgcctt tgaagctgga gtaaaagaaa cgggtgtcac aattcattat
    22381 atcgaccaag gtgtagatac tggaccaatc atccggcagg aaaaagtgag aattgaacag
    22441 gaggacacgt tcgattcttt agaagaaaaa atccatagag tagagcatcg aatctatcca
    22501 gaagtgatta gtgaaattat tgaaaatgga ggctatctga aatgacaaga gcattgatca
    22561 gtgtatcaga taaaactggg attgtagcat tcgcccaagg tttgattgaa caaggagtag
    22621 aaatcatttc aacaggagga acaaagcatg cgttggataa agctgggatc aaaacgcttt
    22681 cgatcgaaga agtgacaggg tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc
    22741 ctaaaatcca tggtggtctt ttaggaagac gcgatctccc tagccatatg gaggcaatgg
    22801 aaacggaaaa tatccaacca atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa
    22861 cgattatgaa accagatgtc gaaatagcag aagctattga aaacattgat atcggtggtc
    22921 ctagtatgct ccgtagtgca gcaaaaaacc atgaatttgt cacctctgtt gttgatccat
    22981 ctgactatga agaagtactt tcagaattac gtcagtatgg gcatacttca ctttctttca
    23041 gacaaaaact agcggcaaaa gtctttcgtc atactgcggc atatgatgca ctgatcgctc
    23101 aatacttaac ggattgggtg gaagaaaaag aaccggaaaa attgacactt acgtatgaac
    23161 gaaaacaaac gcttcgttac ggtgaaaata gccatcaagc cgctgccttt tatcagtccg
    23221 ctcttctgga aaaatattcg atcgcagcag caaaacaact ccatgggaaa gaactttctt
    23281 ataataatat ccgagatgca gatgctgcgc tacggatcat gaaggaatat gacgagccaa
    23341 cggtagtcgc cttgaaacac atgaatcctt gtggtattgg gacagcatct acgattttag
    23401 aagcgtggga agcagcgtat gaagcagatc ctgtttcaat ctttggtggg atcatcgttt
    23461 tgaatcgaga agtagatctg ccaacagcag aacagatgca taagctgttt ctagaaatca
    23521 tcattgcccc ttcttataca caagaagctt tggaattact gcaaaaaaag aaaaacatcc
    23581 gattactgac agtagacttt gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag
    23641 tcttaggtgg acttcttgta caagatcaag atctggcaat agaaaatgaa gaagagtgga
    23701 aagtcgtgac aaaacgtaag ccgactccag cagaagcaaa agcattagcg tttgcttgga
    23761 gagctgtcaa acatgtgaaa tctaatgcga tcgtactggc aaacgaacgt cagacagtag
    23821 gaatcggagc cgggcagatg aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc
    23881 aagagaaaat ggaaggtgca gttttagcta gcgatgctta ttttccaatg gatgattcgg
    23941 ttgaatatgc agccaaacat ggtataaaag caatcattca gccaggagga agcatcaaag
    24001 accaagattc tattgatatg gcgaataaat acgatctagc aatggtcttt actgatgttc
    24061 ggcattttag acattagaga ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg
    24121 gtcgtgaaca tgcaataagt aaaaaacttt tagccagtcc gaaggtggat acggtgtatt
    24181 gtgcgccagg aaatccgggg atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc
    24241 gagatcattc agcgttaatc cattttgtta agaaagaggc tattgattgg agtatcgttg
    24301 gtcctgaagt tcctttgtta aatgggatcg tcgatgattt tatggcggaa ggattgaaaa
    24361 tcttcggtcc gacaaaagca gcagctctga tcgaaggttc gaaaagtttt gctaaacaat
    24421 taatggatac ttatcaaatc ccgacagcta aatccagaag ttttgaatgt tacgaaaaag
    24481 cagcagcgta tatcaaagaa aatggtgcgc caatcgtagt gaaagcggac ggcttagcag
    24541 ctgggaaagg tgtagtcgtg gctaccacag tgaaggaagc gctggatgcg gcagaacaaa
    24601 tgttgaacca gcatcgattt ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg
    24661 caggagaaga attctccctt ttggcgtttg tccgtaatac caaggtgtat ccgatggtca
    24721 tttcgcaaga tcataagcgg gcatttgata acgataaagg gccaaataca ggcggaatgg
    24781 gtgcttattc accagtaccg caaatttcaa aaaagatgat tgaaaaagct gtggaagaag
    24841 ttttaaaacc agcggctttg ggaatgaaag aactaggacg ctctttcacg ggtgttttat
    24901 atgcgggttt gattgctacg aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg
    24961 gtgatcctga aacgcaagtc gttctttctc ggttgaagag tgactttgcg caagtaatcg
    25021 atgatttatt agagaatcgt acagtagagt taaagtggca gcaagaagga tacaatgtag
    25081 gcgttgtggt tgcagctgcc ggttaccccg aagaatatga gacaggaatg gttctaccag
    25141 attttctgga agaagagctt tctgtttact atgctggagt ttcctcaaat aacggcaaac
    25201 tagttggagc aggaggacgt ctttatttag tagaagcata cggtgagacg atcaaagaag
    25261 cacagaaaaa aatttacacc gcgttaatgg ataaaacgac agatggaaca ttttatcgta
    25321 cagatattgg agaaaaagct gccaatagcg aactgatcgt gaagtaaatc agctgcttcc
    25381 atttataaaa aatacaacaa acataatcgg aagcgccgat tttagtgaag ccttttcata
    25441 aagagtgtgg tacacttttg ttgtgtatat aaataaggag gagttcagat gaaaaaaatt
    25501 caattaggta cttctgatca gaaagcagct gcagtgatct taggctgtat gcgtatcaac
    25561 aatgcggaag atccagtaaa agtgattgag acagcttatg aaaacggcat tgattttttt
    25621 gaccatgcag atatttatgg aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca
    25681 aaaacatcaa tcaaacgaga agatcttttt attcaaacaa aatgtggaat cgttccaggt
    25741 gtgatgtttg atttttctaa aaaacatatc attgaatctg tagaaaaaag tttgacccgt
    25801 ctgaaaatgg attatgtgga tgctttactt cttcatcgtc cagatacatt ggtagaacca
    25861 gaggaagtag ctgaagcgtt tgatgaattg gaacggtcag gaaaagttcg ttattttggc
    25921 gtcagcaatc aaaaaccaat gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta
    25981 gtagctaatc agctgcaatt tggcttgaaa catactggaa tgatcgatca agggattcat
    26041 gtcaatatga cagatgaagc aagtatcgat catgacggta gtgtacttga ttattcccga
    26101 ttgcatgata tgacgatcca agcatggtca ccataccagt atggattttt tgaaggtgta
    26161 tttatcggga acgaaaaatt ccctgaatta aaccaaacat tagaagaact agcagctaaa
    26221 tatcaaacga caccaactgg cttggcttct gcttggattt tacgccatcc agccaatatg
    26281 caagtgatcg ccggtacgat gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa
    26341 attaatatgt ctcgggaaga ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct
    26401 taaagattgt cataagcagg tcaaaagggc tataaattaa aaagacagaa taacttgaaa
    26461 gctgttttca gcttcgagat attctgtctt tttttattgc aaatggaaag agtatcattg
    26521 tacggatttc gtttattagt tatttttgtt tttaaggaaa agctgtgtcc gatattcttt
    26581 tggagaaatc ccttgttttt tcttaaataa ataactgaaa tagttagggt cattataacc
    26641 aatttcaaaa gcaatatctg aaatacgata gtctgattgc tgcaataatt tttttgctaa
    26701 ttcgattctt tgttctgtca gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat
    26761 cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg acccaagtta aggacatatc
    26821 tggatcggta aaattttctt tgataaaatc caatgcatgg ttcaaaattg gctgatattt
    26881 gttccttgaa ggctgtgagg gcagagcagt taagaaagaa atgaacatat aaactgtcga
    26941 acggtaaagc gagtcgtcgg cgctgattgc taagcgttga ctggtggatg tgcaatgaac
    27001 catttcttgc gggattttct tttctttcag gcagacatgc tggagtagct tagtcaagac
    27061 aaataaacga tataatcgtg ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat
    27121 gtacttagat acttcgtttt tcgataatga agacaaatca agtgagaggt caaagtaaaa
    27181 gacagaatca actggaagtt tcgcttcttg aatcaaatcc tcatagctga taatttgttc
    27241 tttaagggaa ctggcggttt caagtatcat ctgcgtgatc tcaaaagatg caggtaattc
    27301 gctgattctt tctgcgacag gaccaatggc gacagaaaaa ttattttttt gtcccttttc
    27361 taattcatgg accaacgttt gggctacctg ataactggtc tcgatcacat tttcttcaag
    27421 agaatcaact attaaaaatt tgatgaatcg agaggaaaca gcagagaaga tcacttgttg
    27481 attctcccta aataaaaact tcaaataatc actgagatga gcgtaatctt caaagtctgt
    27541 actgacttgg tttgctgcta ataatacggt catttttttc cctgctaaag agcgattgaa
    27601 agattcagca agtttcaatg tttcactgat cgtaagtgag cctttgaaca aaccgttcaa
    27661 gaaatgattt tttttcaatt cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg
    27721 caaatctaaa gcagttactg ctttttctat tgcttgtttc aattcgctac ttttaatcgg
    27781 tttaagcaga taatcatctg cctgtacctg gattgctcct ttcatataat cgaaatcgtc
    27841 gaatccacta ataaatatga gacgagtcca aggaagtagt ttccttgctt cttttgaaaa
    27901 agtcaacccg tccatgaaag gcatttggat atcagtcaga atgatgtcgg gctgtaagtc
    27961 gataattgaa gacagtgcca tctcaccatc tccagcttcg ccgacataag tgattggata
    28021 ataagaacca aggacagtta tttgttcccg caagttattg cgaatgaggt attcgtcttc
    28081 gatgataaaa actttataca tgtttgtttc ctccaattgt tttgggagat agagggatca
    28141 tcagtgtgac agttgttcct tctcggtatt tgctgtcaat cgtgataggt ttttgatcat
    28201 ttccgtaata aagaaggaga cgcttgttta cgttgtaaag tccgtagccg atactgaaat
    28261 ctgaatcgat ccctttggct aactctttac gtatttctgc cagtttttct gcagggatgc
    28321 caatcccgtt gtctataact ttgaattgga tcatctcatc aattttgcat gtggtaatac
    28381 gaataaatcc tttgtgacgg acgtacttcg ttccatggta tacagcattt tcaatcaatg
    28441 gctgcaagat catttttaaa atagaaacag gaaaaagtgt ttctggtata tcgatcttat
    28501 agtcaagcat atggccgtat cttattttta agatgactag ataatcattg atatggctaa
    28561 tttctttttc aagaggaatc cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg
    28621 aaagtgcatg ggtcatagtc agagcttctt caaagcagtc ttgttcgatc aaagaaataa
    28681 tggcatccaa actgttataa ataaaatggg gagtaatctg agcttgcaat acacgaactt
    28741 cactttgtgc taaatggtat tgtttcaaag cgttctcccg taaagactgg tttagatctt
    28801 ccgccatttt attcatgcta gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta
    28861 aacgaaagcc aaggttccct gaagaaagtt gttctgacat ctcgatcagc tgattgatcg
    28921 gttcttggac ctttctctct aaaaagcgac gattcttttt cgcatagaaa aaaatggtac
    28981 taagaactcc aagttccact attgttaata gaatgagcga gcggacaagt gtcgtatttt
    29041 ttttgcttgc taagtcgatc tccacacgaa caaaatcttg taagatatct gacagcagtt
    29101 gagtgacaga atctacctga ttgatgatct cttcattggt atccataaaa tcttttgcca
    29161 agatattatt gatcagctcg ttttgataat cttccagtgt atcgatcgta cgtagcgata
    29221 catctaacgt actaatttct ttaagtgtat ttgtattatt tttgatctct cgaatatcat
    29281 tcctaagttc ttctaggata ttttcctcat tgaactcatc tacggttact tggccagaaa
    29341 tcaagtccca catactttcc aaaacttttt gatcaacctt caaagcaatt gcatctgctt
    29401 tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc
    29461 tcagtagcaa agggataaca gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct
    29521 gttttatgct taaatttttt gtcatgatac ttctccagtc aataatttct ggttgggatg
    29581 ttagctatat tctgctggga aaagacagtc tcggaaataa aaatatcttt aggtaatgtt
    29641 tttcctgttt gaatatttcc aaaataacga gtaacggtat tggctacata ccagccgaca
    29701 tcaggtttac actcgactac acaattgacg attcctgagc gtagcttctc gatcatatct
    29761 tcttgtccat caatagttac aatgatcaaa tcttctccag gtttgattga tgtttctttt
    29821 atcgcagcta aagcaccgtg agtcatttca tcactatggc tgtataatac atcgatttcg
    29881 gataaatcat ttgtttctaa atatcgtttc atctggtctt ttcctttcat ccgaataaaa
    29941 tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt tccatctttt
    30001 tttagggttg gtttacgatt gattgattcc aaaaatcctt cggagcgtag cttggtcggt
    30061 gtggagttct ctgaaccagc aagttctagg attctgattt cctgtttctt tgaatttgtg
    30121 aagtgattgc tgacaaataa acccgcccga tttccttctg ctttgaaact ggggccaatg
    30181 tgggtgacga aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg
    30241 atccctgctc gctttgcttc ttttaataca gagtcccacc cggattcttg caaaggagta
    30301 aaaatgatca tatccacttt ataggcaatg aacgtccgta tatcttggat ttgccgttcc
    30361 tgattcatgt agccatttcg gtagagaact tggtaatctt ctttttcaag cgcagtgata
    30421 attgattcag tatgcttttt ccgccagcta ctctccgttc ctgattgcga aaaaccaacg
    30481 actagtgggt caggtgcttc ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt
    30541 acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa atcactccat ctggatttgt
    30601 tagtactatc ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa
    30661 tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat
    30721 cccataaaaa aatcaatgtt aacgctttct ttattagcta tactcggtgt atcaattgaa
    30781 atgggggata aaaaatgaaa aacaaattta gaaagattgt tgggactatc gctattttga
    30841 ccgcaagcat tctattaagt gcgtgtggca attcaggatc tgcagatgat agtacggggt
    30901 atgtaggaat cgctatgccg accaaatcag cagaaagatg gatcgctgat gggaacaaca
    30961 tggtctcgga attagaaaaa ctgggttata aaacagatct tcaatatgga gaagataaag
    31021 tagaaaatca agtagcacaa atagaaaata tgattacaaa aggggtagat acacttgtga
    31081 ttgcttctat cgatggttcg gctttaactg atgtgttagc taaagcaaaa gaagcagata
    31141 tcaaggtgat cgcctatgat cgtttactga tgaattcaga aaatgtcgat tattacgcga
    31201 cttttgataa ttttggagta ggtgtatcgc aagcagctta tattgaagag cacttaggat
    31261 tgaaagaagg aaaaggacca ttcacgattg aattatttgg tggctcgcct gacgacaaca
    31321 acgcactaat caattacaac ggagtgatgt ctgttttaca accatatatg gacaacggtc
    31381 agttggtcgt gccatctgga cagacgagtt tcagtcaaat cgctacgctt cgctgggatg
    31441 gttctacggc gcaagcaagg atggataatc tgttaagcgc aaattatact gaccaaacat
    31501 tggatgctgt tttgtcgcca tatgatccaa taagtttagg aatcatttcg tcattaaaag
    31561 gcgtggggta tgggagtgaa agtaaacctt tacctgtgat tactggacaa gatgcgacag
    31621 ttgctggcgt taaatcgatc attgccgggg aacaaacaca aacgatcttt aaagacacac
    31681 gtatcttagc aaaaaatacg attgaaatga tcaaagcaat cagtgatgga gaagaggttc
    31741 cggtcaatga tactgaaact tatgataatg gggtgaaaac tgtccctacg tatttagcaa
    31801 atacagtatc tgtcgataaa gataattatc aagcagagct gatcgacacc gattattata
    31861 aagaatccga tctgaaaaat taagaggaac caaacaagga actaaagaga tctaagtcag
    31921 agagaggata agaattatgg cggattgtat tttaaaaatg gcagatatca ccaaaaaatt
    31981 ttctggaatc cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc
    32041 attgtgcgga gaaaacggag cgggaaaatc aacattgatg aatgtcttga gcggcttgta
    32101 tccatatggc tcctatgacg gggaaatctt ttatgatgga aagctgtgtc aatttaaaga
    32161 tttgaaagat agtgaagaaa aaggcatcgt catcattcat caagaattag cactaagtcc
    32221 ttatctttca ataaaagaga atatttttct cggaaatgaa caagcagaaa gaggcgtgat
    32281 tgattgggaa ctgacagaaa agaaaacata tgaactgctg aataaagtcg gtttgtctat
    32341 cgacccgaat cttctagtat cccagattgg cgtagggcaa cagcaactag ttgaaatagc
    32401 taaagcatta tcaaaatccg tccgtctgtt gattttggat gagcctaccg ctgcattgaa
    32461 tgaagaagaa agcgcgaatt tgctgagatt gatccgagag ttgaaagagc aaggtgtaac
    32521 ctccatcatt atttcacata agctgaatga aatcgtggat atcgcagatc gaatcaccat
    32581 tttacgggat ggacagacga ttgagacact agaaaatgaa atgatcagcg aagagcatat
    32641 tattcgcgga atggtgggca gagatttagt tgatcgttat cctgaaagaa aaccggatat
    32701 tggagaagtt tattttaaag tagaagactg gacggtctat caccctcttg atcacgagcg
    32761 aatcataaac gaccacatca acttgcattt gaaaaaagga gagattgtag gaatagctgg
    32821 actgatggga gcgggcagaa ctgaattcgc tatgagtcta tttggacatt cttatggaag
    32881 caagatatcc ggacgtatat taaaggaagg ggcagagata tcagttaaga ccgttccttc
    32941 agccattgca aacggactcg cttatgtctc ggaggaccga aaatctttag gactgaatct
    33001 gctgatggat attcgggaaa acgtgacgct gagcagtttg gataaaataa gtcaaaaagg
    33061 agtattagat aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa agatgcgtat
    33121 aaaaacaaac tccatccatc aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt
    33181 cgttttatcc aagtggctga tgacacaacc cgatattctt tttttggatg agccaacccg
    33241 tgggatagat gttggggcaa aatatgagat ctacacgatt atcgaagaaa tggcagcaaa
    33301 tggcaaagct gtctgtctta tctcttctga acttcctgaa atattaggga tgtgtgatcg
    33361 aatctatacg atgaacgaag gacgtattac aggagaagtc tcgcgagaag atgcagatca
    33421 ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt taagttggaa caggcaaaaa
    33481 atcttacgga taaaaaagag aaaaaactaa atttgaaaag tagagcagct gatctttttg
    33541 gtcgctacag catggtgatc attttagcgg gactacttat tgcgtttcaa ttgatgacgg
    33601 atggtatttt ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat agccatatat
    33661 tagtactagc tgcagggatg ttattagttg tgctcttagg tcatgtggat ttgtcggtag
    33721 ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat cgccaaatga
    33781 acccgtggat gacagttatc gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag
    33841 gtttttgggt agcttatgca gggataccgg cgtttatcgt gactttagca ggattgctga
    33901 tgtttcgtgg actgacacaa gttgttctag gaggccaatc tctagcacct tttccagctg
    33961 ttttccaaaa aatgtctacg ggttatttac cagacttaag cgaaggaagc agccttcact
    34021 tcttgacatt gattttagga atgatcatca cgatcgtgtt agttgttggt caatggcgaa
    34081 caagaaatcg tcggaaaaat catttatttg aagtggaatc tccaacagct ttcttggtaa
    34141 aggcggtatt tacagcagtc gttgtactag gaataactta tatctttgcc tcttatcaag
    34201 ggtatccgat tattttgatc attcttggtt tgatcgttgc agcatatggg tttttgacaa
    34261 ataaaacagt agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac
    34321 tatcagggat caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg
    34381 cagctttagc tggattagtt ttagctgctc gattgaatgc ggcaacacct caagcaggta
    34441 catcgatgga actggatgca atggcggcag tatattttgg tggtgcttcg acttccggcg
    34501 gcattggaac aatcatgggt gcgattgtcg gtggcttggt catgggtgta ttgaacaatg
    34561 gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc aatcaaagga ttgatcttgc
    34621 ttttagccgt tgtgctagac ttgtataata agaaaaaacg aacaacgtag gagaacgccg
    34681 gttattcaga aaaaagactg tgacaagaca tttgtcacag tctttttttc gctaaaagcg
    34741 aacattcatt gtttcgtttg tatagactaa aaataatata tgttttttta ccattcgtat
    34801 aaattctctt tttgctcttg atttttttgc ttaattgagt taaattacat agggcgctaa
    34861 aaaaagcctt gattaaatta ataatgttcg tgttttgctg gatgtgaaca tcaggaggat
    34921 aatgtaatga aagtatttga ttacgaagat attcaattaa ttcctaataa atgtatcgta
    34981 aatagccgtt ctgaatgtga tacaacggtc acattaggta aacatacatt caaaatgcca
    35041 gtagtgcctg ccaacatgca gacgattatt gatgaaacaa tcgcagaatt cttagcggaa
    35101 aatggttatt tctatatcat gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa
    35161 aaaatgaaaa agcgtggatt gattacctcg atcagtgttg gcgtcaagaa agaggaatat
    35221 tcctttatag agaagttggc agaagaatcg ctaaatcctg attatatcac tatcgacatc
    35281 gcgcatggac atgctaactc agttattgat atgattcagc atattaaaaa atatttgcct
    35341 gaaacttttg tgattgccgg taatgttggt acaccggaag ccgtccgtga attggaaaat
    35401 gcgggcgcag atgcaacaaa agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc
    35461 aagacaggct ttgggactgg aggctggcaa ctggcagctc ttagatggtg tgcgaaagca
    35521 gctcgcaagc cgatcatcgc tgacggtggg atcagaacac atggcgatat tgcaaaatct
    35581 gtccgttttg gtgcgacaat ggtcatgatt ggttcattgt ttgccggtca cgaggaatct
    35641 cctggcgaaa caaaagtcga aaatggaatc gtgtataaag aatactttgg ttctgcttca
    35701 gaattccaaa aaggcgaaaa acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa
    35761 ggatctttga aagatacact agtggaaatg caacaagacc ttcaatcatc tatttcttat
    35821 gctggaggaa gagatctgga agccattcga aaagttgact atgtcatcgt gaaaaattcg
    35881 atttttaatg gagacacaat ctaaaaaata aataaaatat taggatgttg tgaaaaagtt
    35941 gtcctgcatt aagtaataag aacagccaaa caaaaatagt tctttatatg ttttgtttgg
    36001 ctggaattat tatcgcagat acaagctttt gaacatcatt attcagaata agggactgtg
    36061 ataagatttt tgtcacagtc ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa
    36121 attcggtaaa atcaaaagaa aaaatagaca atagaaaaga gaagcttatg tgggaaaaag
    36181 tagagaaaaa acaaggaccg atttatcgtc aaattatgga acagattatg agagaaatcg
    36241 aaaacggaag gatcaatcct ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt
    36301 ttggtgtgaa tcgttccacc gttgtccatg cattggaaga gcttagggag cttggggtac
    36361 taacgagcaa acgtggcagc ggatggtttg ttaatcagac tgaatggggg aaattcgctg
    36421 ttccaagaat cgattggcgg caaatgatct ctcctcgtta tgagcagacg gactggtatg
    36481 agcaaaaaat aagagaagca aagcagatcc aaaaaacatc gtttcttgat ttatacgcta
    36541 gcgagatgcc agatgagctt ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa
    36601 tcttagcgga agaaaaagaa atggatcttt taggctatac tccgttacgt aacaaaatca
    36661 aacggtattt ggaaagtaaa tttgaattgg cattttcccc caaccagctt cttgtgactg
    36721 gtggcggtca gcaagcaatc tttttgattt tacaaacgat tctttcagct ggagagggga
    36781 tagctgtaga gtcaccttct tttttttacc gattacctct tttcaaagct tcaggaacgc
    36841 gtttatttgg gataccgatg gatgaagaag ggatcgactt agaaaaactg aaagaatcga
    36901 tccataaaaa taaattgaaa gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa
    36961 aagtgatgag tcaaaaacga cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc
    37021 caattattga agacgatgta tttacggatc tttcatttct aaaccaagaa gaagtccttc
    37081 ctatacgaac gattgatccg gataatgtgt tatacgtcgg ttcgttatct agggttttag
    37141 gaaaaacaac gaaaatcggt tgggtgatag gacctgagac ttttatctcg caactagctc
    37201 aagcgcaaga aatgatggag ttttcgatga atatccttac acaaatcaca gtggctaatg
    37261 tatttaatga agcaattgat ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa
    37321 gccgactgat aagcgcttgg ggagattcac aatcgttttt caagcttcat tcaattttag
    37381 gtggttacta tgcctggatt acttgggatg ggaaaaaact agaccccgaa ctagcagaaa
    37441 aagtagtaga tatgggtctt ggaattgcac caagtctact ttttgggcaa gatataaatg
    37501 gaatcagaat caattacagt aggctggatg aaaaccaaat accgttgttt tattcaaaga
    37561 tgaaaattct agaaaagtgg ttaacagaat aatttcacaa agtaaataga ttaaagaata
    37621 taacagcttg gatttaaatt tcaagctgtt tctttttttt cggaaaaaga atacgatata
    37681 aagaaactaa gaaaaaacca ttatttgtac gtccgttcac gatgtgtaaa taaaataaat
    37741 gtttgcaaca aaactgtata acaccaacgt ttttaaatag aaacagatgg ttgtaagttt
    37801 gattagaaaa tagaaaaaat gcagatgaaa tataaaacgc tttccttatt gtgattttat
    37861 tcactaatgc tgttaatatg cggattggca taatttcctt tcatcgtgaa aatttgtgct
    37921 tttagcttgt atttctcata aatccatgat aatttattaa tgaagacggt taaatagtga
    37981 tacagtttta ttcactgttt caatcgcaga atgaagagat tttcttatag atgagagagg
    38041 aatgtgtgac aaatggcaaa caaaaaagca ccgatcgact tccaagcact gctagatgaa
    38101 gtcaatgcag atttcccagt atatcaagca cttgatcaag atggaaacgt agttaacaaa
    38161 gatttagtac ctgatttatc cgatgatgaa ctcgttgaat taatgactca tatggtatgg
    38221 tctcgtgtat tagaccaacg ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt
    38281 gcaccaactg ctggacaaga ggcaagtcag ctggctagtg cttacgcatt tgataaagaa
    38341 gatgttcttc ttccaggtta tcgggatgta cctcaactag taaaacatgg tttaccttta
    38401 tctcaagctt ttttatggtc tcgaggacat gctgcaggta attcttatcc ggaagaattg
    38461 aaagctctac caccacaaat tatcatcggt gcacaatatg tacaagcagc cggtgttgca
    38521 ttaggtttga agaaacgaaa caagaaaaat gtcgtcttca cttatactgg tgatggcggt
    38581 tcatcacaag gtgattttta cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc
    38641 gtattttata tccaaaacaa cgggtatgcg atctctacac ctcgtgaaaa acaaactgca
    38701 gcaaaaacac ttgcacaaaa agctgtcgca gctgggattc ctggtattca agtcgatgga
    38761 atggatccgc ttgcagtata tactgtatct aaaatggcac gggaatgggc gatcagtgga
    38821 aatggaccag tcctaatcga aacactgact tatcgttatg gaccacatac actatctggt
    38881 gacgatccaa cccgctaccg tacaaaagac acagatgacg attggcagaa aaaagatcca
    38941 ttgatccgct tccgtaaata tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa
    39001 caagtcatcg aagcaactaa agaagaaatc aaagcagcta ttgctgaagc cgacaaagta
    39061 ccaaaacaaa aggtttcaga tttcttgaaa aacatgttcg aagtatctcc acaatcaatc
    39121 aaagaacaaa ttgcaattta tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa
    39181 tgatccaagc aatcacagat gccttagcac ttgaactaga aaatgacgag aatgtcgttg
    39241 tttttggtga agatgttggg aaaaacggag gagttttccg ggcaactgaa ggattgcagg
    39301 aaaagttcgg agaagaccga gtattcgata ctcctttagc tgaatctgga atcgcaggat
    39361 tgagtttcgg tcttgcgtta gaaggatttc gtccagttcc tgaaatccaa ttctttggtt
    39421 ttattttcga agcaatggac gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga
    39481 gcggcactag aaatctaccc atcacaattc gttcgccatt tggtggcggt gttcatacac
    39541 cagaattgca ttcggataat ttagaaggtt tgatcgctca gtctcctggt attcgagtag
    39601 tgattccttc caacccttat gatgcaaaag gactattgat ttcttctatt agaagtaacg
    39661 atccggttgt tttcctagaa cacatgaaac tatatcgctc attccgagaa gaagtgccgg
    39721 atgaagctta cgaagttcct ttggataaag cagcagttac tcgtgaggga acagatgtat
    39781 ctattatcac atacggagca atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa
    39841 aagaaaatat atctgttgaa atcattgatc ttcgtacagt tgcgccatta gatgtagaaa
    39901 cgatcattca atccgtggaa aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac
    39961 aagcgggcgt tgccgcgcaa gtagtttctg agatctcaga acgcgctatc ctttcattag
    40021 aagccccaat cggacgagtt tctgcacctg atacagtctt cccatttgga caagcagaaa
    40081 atgtctggtt gccaaatgca aaagatatcg aagacaaagt aaaagaaatc gcggaatttt
    40141 aagaaaagaa gggacgaaac aaagtcatgg cttatcaatt taaattgccg gatatcggtg
    40201 aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat acgatcaatg
    40261 aagatgatac attactagaa gttcaaaatg acaaatcagt agaagaaatc ccgtcacctg
    40321 ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat gttggggatg
    40381 tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt ggtgtagccg
    40441 cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca gaatcagctg
    40501 gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa ggaatcgcag
    40561 aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa gatgatacat
    40621 tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc acagggacag
    40681 ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta ttagtagaaa
    40741 tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct gaagcaccaa
    40801 aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat ccaaataaac
    40861 gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta gatatctcac
    40921 aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa aacttcttgg
    40981 caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca gcaccgaaag
    41041 aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc aagtccaact
    41101 taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc gctaaagcaa
    41161 tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta gaagtgtcta
    41221 aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt acgaaactta
    41281 ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac ccagtattga
    41341 atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac aatatcggta
    41401 ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca gaccgcaaag
    41461 gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat gacggcaaac
    41521 tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga tctgtaggtg
    41581 gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt gtaggaacga
    41641 ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt gtgatgaaac
    41701 tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa gcaatgaata
    41761 atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga taaacaaatg
    41821 gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg acctggtggc
    41881 tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat tgaaagagag
    41941 tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact gatagctgca
    42001 ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag tgaaaacgtc
    42061 aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca tacgttgact
    42121 tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg agaagcgttc
    42181 ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac ttattcgttc
    42241 aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt taaattcgga
    42301 ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa gaaatttgtg
    42361 atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa cttaggagca
    42421 gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa agatctagta
    42481 aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa tgcaatggcg
    42541 aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga cgggaaagaa
    42601 gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa tacagatgat
    42661 atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac ggtggataaa
    42721 caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc aggggcggca
    42781 ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc tggcaaaaaa
    42841 gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga attggcttct
    42901 gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc atataaattc
    42961 ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt catccgtcta
    43021 gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt cggcgcaagt
    43081 gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga agatatcgcc
    43141 ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc ggaattagca
    43201 ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac tgaaaaatca
    43261 gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa aaatatgaca
    43321 acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa ataggagagt
    43381 tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac gaaatggtca
    43441 ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata aacaatgaag
    43501 cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt gaggaaaaac
    43561 gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca gtgcagcaag
    43621 caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg aacagatgat
    43681 tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt ctcaacaaac
    43741 attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg atcgaaacat
    43801 ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa tattggcaga
    43861 agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca tcgatcctat
    43921 tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc tcgctgttta
    43981 tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg aacttttttg
    44041 gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac cagcaaataa
    44101 atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata ataaatatgg
    44161 aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg ctggtcttga
    44221 actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta gcccttggga
    44281 ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg gtctttttgg
    44341 tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt gtgcatatgc
    44401 agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc attcacaaaa
    44461 atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt tactatattt
    44521 catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca taaagaacca
    44581 ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt cgcaattatt
    44641 gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca atcagataca
    44701 ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct agaaaaagaa
    44761 cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat tcgcgtaaac
    44821 atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat catgaaaatg
    44881 gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca aacacgtttt
    44941 gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa caagattgac
    45001 aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt catcgaatta
    45061 ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact aaatggtacg
    45121 tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat ttttgataca
    45181 attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca attccaagtt
    45241 tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt atttagagga
    45301 acaatcaaag taggagacca agtagcatta attaaattag acggaacagt taagaaattc
    45361 cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca agaagcaaaa
    45421 gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga aacagttact
    45481 cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac attgcaaatg
    45541 actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt aaccgctcgt
    45601 aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg agttgaacca
    45661 acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt gtctatcttg
    45721 attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga agttattgaa
    45781 aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga tacaccagaa
    45841 gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat gcaagatatg
    45901 gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg cggtttgatt
    45961 ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa ccatactttt
    46021 gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg ggcgcttgtt
    46081 tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga acgcggtaca
    46141 gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga aaacagccgt
    46201 gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt acggtctgct
    46261 actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga agaatctttg
    46321 gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg cttgagaaaa
    46381 caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata atctgtaaaa
    46441 ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt tgtccaactc
    46501 ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac gccgaaagac
    46561 gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa taagataatg
    46621 tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg gcaagagaaa
    46681 ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag tttagacctg
    46741 gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga atcatgccta
    46801 ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat gggcttactg
    46861 tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg aatcaattag
    46921 gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct agacgcttcc
    46981 aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt tttatcatgc
    47041 cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt gacgaggcac
    47101 attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg gcgaaccagc
    47161 tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg actgtgcaag
    47221 tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt cagactggtt
    47281 ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa caatatctaa
    47341 tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca acgagaaaag
    47401 aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt agatatcatg
    47461 gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac gatcgcttgc
    47521 agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat gtccgttttg
    47581 ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct ggtcgggcgg
    47641 gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat atccaagtcc
    47701 aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa gaatatgaaa
    47761 aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa tacatcctaa
    47821 actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta gatgacagag
    47881 aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa cgtatgggtg
    47941 agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa caaaagctgc
    48001 aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa tcgcaaaaag
    48061 aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc atcaatggtg
    48121 agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga caagaatctg
    48181 tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc gatacactat
    48241 ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt cccccgtatg
    48301 tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca agcagattgg
    48361 agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc gaagcctttt
    48421 tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa cgaatacgta
    48481 aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa aatcaaatat
    48541 gttataatta aggaattaga atgggaggta agcacagatg gattctttgt cgaaaaaact
    48601 tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc gaaatcaaaa
    48661 caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata cacagatgta
    48721 cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa agaacgaggg
    48781 ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg aggtttacca
    48841 agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa gaaagtgaaa
    48901 aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg tgtattggga
    48961 ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca aaatccgtcc
    49021 gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat cgcactactt
    49081 tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc catcgcagtt
    49141 ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg ggcgaaaggt
    49201 tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat catttctatc
    49261 tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga ttttcttggg
    49321 actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc tattcttccc
    49381 gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt agccagcgga
    49441 gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag tacttttacg
    49501 atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca gtatatctat
    49561 aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg gcatcaacta
    49621 gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt aggtaacagt
    49681 atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc aatcgtagtg
    49741 gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt tatgattgtg
    49801 cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt gtgcattggg
    49861 atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac tggcgttatt
    49921 cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt gctgatgctt
    49981 tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa gagcttaggt
    50041 ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg ctaacagggg
    50101 tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa ctgttggaat
    50161 ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag catatttagt
    50221 aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca tttctatcgc
    50281 gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg aaaacttgaa
    50341 ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac tgcatcattt
    50401 agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg ggattgcttc
    50461 gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt ttgcgaaaca
    50521 atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg gcatgcgtgt
    50581 agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg aagcaaaagc
    50641 agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta agcatatcga
    50701 agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa gagattgttc
    50761 tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta tgaatgaaca
    50821 gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg ggtatgtcaa
    50881 cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg aagtcaaccc
    50941 tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata tcgtaacaac
    51001 gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc ctcagcaaga
    51061 aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag acccgttgaa
    51121 taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg gttttggcgt
    51181 acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt ttgattcatt
    51241 gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga aaatggaacg
    51301 ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta tgctcaatgt
    51361 catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg atagtacagc
    51421 aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga aatatatcgg
    51481 tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat tttatgaaga
    51541 accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta agaatgtatt
    51601 agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa atttgttgtt
    51661 gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg ttcggacaaa
    51721 ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct tttcaagtga
    51781 gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag atccttggca
    51841 acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc ttttcagagg
    51901 ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat ttgtcaaaga
    51961 agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact ggactccaca
    52021 aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg cagctatttg
    52081 ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt attataaaga
    52141 catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag atatggctgg
    52201 gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa cgacagatct
    52261 gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt attctgctgc
    52321 ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg ggaatactag
    52381 tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc tgccagaaat
    52441 cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca tgtattacaa
    52501 accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg aaatgccagg
    52561 aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc atcgctggga
    52621 tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg ttaaagtaac
    52681 tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg atcttacaga
    52741 agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg tgactttctt
    52801 ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa agattattct
    52861 taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag tcgacttcaa
    52921 cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag acgaagtatt
    52981 aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata atcaatttgg
    53041 cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg gtgaaacaat
    53101 caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa tcggagaacc
    53161 cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac gtgaaatcac
    53221 tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg aaccgacaaa
    53281 ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag tgaaaaaagg
    53341 cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa tggaaacggc
    53401 catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag gagacactat
    53461 ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag gtggggaaac
    53521 atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc attctacatg
    53581 gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca acaaatatca
    53641 aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc tacttatatc
    53701 ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg attaactagc
    53761 ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac atttgaagcg
    53821 aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc tgctgatatc
    53881 gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac tatctatact
    53941 aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga agaggatatg
    54001 aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt ttttgatcaa
    54061 acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt gaaactattg
    54121 ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc ggctgattgg
    54181 gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat gcttagaaga
    54241 gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag tcagctgcta
    54301 ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat ggctgattgg
    54361 caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa tgatgaattg
    54421 gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat tcatccggcg
    54481 attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca tgaccgtttc
    54541 atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat gttagtctta
    54601 ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag agctgtttca
    54661 actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa cgatggcttc
    54721 ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt tgaaagaagc
    54781 gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt atgcacctga
    54841 ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata tggatgagca
    54901 tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc aaattgtcca
    54961 agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc cgttttttga
    55021 aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat agtgaatttg
    55081 aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa ttaaagaatc
    55141 tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc gtgctttact
    55201 taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat tttgttcgaa
    55261 acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa ataggaaaca
    55321 aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa atccgattag
    55381 ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac ttggataacg
    55441 atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt accatttcta
    55501 aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt aaaaagttta
    55561 gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt caatatgatt
    55621 ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg tggtttagct
    55681 atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa ttaccaagcg
    55741 ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt taccacaata
    55801 aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca attcgactac
    55861 ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca taaaatggaa
    55921 gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa aacagtcgaa
    55981 ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg cataacggcc
    56041 gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca ggaagttttg
    56101 atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta ttcgatgagg
    56161 tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca gaagaaagaa
    56221 tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg catcaagaaa
    56281 atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc agaggcatac
    56341 gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat catctatatg
    56401 atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg agttccagta
    56461 ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg ccagtgatca
    56521 acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt gaaaacagta
    56581 ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt gccgtattat
    56641 gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa tgataaggaa
    56701 gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc tacaggtttc
    56761 ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga agaattgact
    56821 ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga ttcttcccaa
    56881 aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat ggaattcaaa
    56941 ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct ttcagttgga
    57001 gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt tatggattat
    57061 gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa tggtgaagaa
    57121 aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg aataggtatc
    57181 acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa ttctggctct
    57241 atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca agtcactggt
    57301 aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag tgaaggagaa
    57361 gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc tggcgtagag
    57421 attttctttg caccagatga tacgatttca gacgatgtta agaaagaata tcctgaaata
    57481 aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc aatgaaaatc
    57541 gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa atgaaactaa
    57601 ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc gtcaaaaatc
    57661 ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg aagggacgat
    57721 tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat gatttttact
    57781 ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata ctagcttcac
    57841 ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca agtggttttt
    57901 tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa gaaatacttt
    57961 cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta gaagaggatg
    58021 ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct ggtatctatt
    58081 ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga ttattagaag
    58141 aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa gtcctatata
    58201 tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc tcggaaggaa
    58261 aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc actccatcag
    58321 aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt cgatttagag
    58381 aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt atcggtgaaa
    58441 agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca aaatcgactg
    58501 gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta agagcttggc
    58561 aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa agaaacatta
    58621 aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca tattatgaat
    58681 acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa agatgtactt
    58741 agctatacag cagataaagt atttattctc attgggacga atgatctagc agagcataaa
    58801 caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag ctgaaaaaag
    58861 actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag acaaaacaaa
    58921 aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa gtagcaaaac
    58981 gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg attcgaagaa
    59041 tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt aggatctttt
    59101 gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg agtacttttt
    59161 aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca aaagcatgcg
    59221 atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct tgggaccagt
    59281 actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt ttagaagtag
    59341 gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga gcagtcagtg
    59401 gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc ttgcgaacga
    59461 tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg acggaaggtt
    59521 cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg ttgcaagctg
    59581 gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc caagcgttga
    59641 tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat ttttcaaaac
    59701 ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt tcgaaatcgg
    59761 ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac tgacaattgg
    59821 ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga aggacgtttt
    59881 atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag gctacaagag
    59941 tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac agatacgatt
    60001 cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa acaaaagctc
    60061 cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa cttccgagga
    60121 aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa gcggaatctt
    60181 tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg gttccaaatt
    60241 caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca gagaaaaaga
    60301 ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt atatattaat
    60361 tctttattat ggggctataa agatgcagga ttcagagaac cagaagaaat ttttagggag
    60421 agtggtttgc tgcatctgtt cagtctatcg ggtctacaca tccaatttta tttagggtgg
    60481 ctgtattatt tgtttcgacg tatcggatgg ccactacacg tcagtattat tccgttgagt
    60541 ctactaacta tctgttatgt ttctattgca ggaagttcta tttctgtttt gcgtgctagt
    60601 ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata agcattattc caccatggac
    60661 ttatttgcag ttgtcttttg gcttctttta ctatttgatc ctcgatttct ttttcatgcg
    60721 ggtggacagc ttagtttttt catgagtttt ttgtttatcc tgatttcttt tgatggagat
    60781 tggttgaaaa aaacggcctc tcacgtcctg tttactatga tcacgatgcc gttgattgta
    60841 tggcattttt atgagtggcc tattttaggt agtcttttta cattactgat tgcaccggta
    60901 ttcaagtttc tttttcttcc tggtgtgtta tttctcacac tcttcaatcg ctggctgccg
    60961 gaaactttac ttttgctgat agaagaggga ttaatcttat ttgagcgaat aatcgatttt
    61021 tcttcagggt cgtctctcat tataggacgt ttgccccttg tgctatctgg gatgtatatt
    61081 ttcggattac tgatttgtat ggataggttg aaagaacaac ctgtgaaaca cttgttttat
    61141 gttggtcttt tttcttttat ttttttgact ttgccatttt tacgcttttc ttcaacgatc
    61201 gcttttatcg atgtcggtca aggagacagt atctttttgc aaagtccttt tcgaaaagaa
    61261 acgattttga tcgatactgg aggtcagtta cactttgaaa gagaaaagtg ggcgcaaggt
    61321 atgatacgtc ctcctgctga aagaaatatc gttccatatc taaaaggtca ggggataagt
    61381 gtaatcgata aactcatctt gacacacgac gatacggatc atgtgggaga attacccacg
    61441 ttatctcagc attttactat caaaaaaatt tatatcggtt ggggcgctgg acgaaatgag
    61501 ccattacaaa ggcatttatc tgaagctcaa aaaaatggta ctgagatcat tgaaatcaaa
    61561 catggggaca ggatcagcgg ttattatgat ctttatgtat tgactccttt tgaaaaggga
    61621 gagggaagaa atgaagattc gattggtcta tggatggaat ataacaatag gcgcttttta
    61681 tttttaggag atttgaatca agcaatggaa aaacaattgc ttttgattta tcctaatctg
    61741 aaagccgatg tagtcaaatt aggtcatcac ggtagccgta cgtcaagtaa cacagatttt
    61801 ctttcccata ttgaagcaaa acatgggatt atatcctgcg gcgtaaataa tcgttatggt
    61861 cacccgcaca gcgaagtatt ggagacacta gaagaaaatc agataaaaac atggcgaacg
    61921 gatcaacaag gaatgatcac ctatcgctgg catccagttt
//
LOCUS       DAYLZO010000005        54824 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.005, whole genome shotgun sequence.
ACCESSION   DAYLZO010000005 DAYLZO010000000
VERSION     DAYLZO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.005"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..886)
                     /locus_tag="ACGVJG_000261"
     CDS             complement(<1..886)
                     /locus_tag="ACGVJG_000261"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFJ6556249.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFAC"
     gene            complement(873..1838)
                     /locus_tag="ACGVJG_000262"
     CDS             complement(873..1838)
                     /locus_tag="ACGVJG_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556250.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(1835..3373)
                     /locus_tag="ACGVJG_000263"
     CDS             complement(1835..3373)
                     /locus_tag="ACGVJG_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFJ6556251.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(3789..4076)
                     /locus_tag="ACGVJG_000264"
     CDS             complement(3789..4076)
                     /locus_tag="ACGVJG_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556252.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            4210..4878
                     /locus_tag="ACGVJG_000265"
     CDS             4210..4878
                     /locus_tag="ACGVJG_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFJ6556253.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(5169..5909)
                     /locus_tag="ACGVJG_000266"
     CDS             complement(5169..5909)
                     /locus_tag="ACGVJG_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556254.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(6039..6986)
                     /locus_tag="ACGVJG_000267"
     CDS             complement(6039..6986)
                     /locus_tag="ACGVJG_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6556255.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(7115..7879)
                     /locus_tag="ACGVJG_000268"
     CDS             complement(7115..7879)
                     /locus_tag="ACGVJG_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFJ6556256.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(7883..8272)
                     /locus_tag="ACGVJG_000269"
     CDS             complement(7883..8272)
                     /locus_tag="ACGVJG_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556257.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            8407..8949
                     /locus_tag="ACGVJG_000270"
     CDS             8407..8949
                     /locus_tag="ACGVJG_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFJ6556258.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            9097..9729
                     /locus_tag="ACGVJG_000271"
     CDS             9097..9729
                     /locus_tag="ACGVJG_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556259.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(9863..9988)
                     /locus_tag="ACGVJG_000272"
     CDS             complement(9863..9988)
                     /locus_tag="ACGVJG_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFJ6556260.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(10122..11015)
                     /locus_tag="ACGVJG_000273"
     CDS             complement(10122..11015)
                     /locus_tag="ACGVJG_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFJ6556261.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(11197..12123)
                     /locus_tag="ACGVJG_000274"
     CDS             complement(11197..12123)
                     /locus_tag="ACGVJG_000274"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFJ6556262.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(12208..12969)
                     /gene="pflA"
                     /locus_tag="ACGVJG_000275"
     CDS             complement(12208..12969)
                     /gene="pflA"
                     /locus_tag="ACGVJG_000275"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFJ6556263.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(13213..15444)
                     /gene="pflB"
                     /locus_tag="ACGVJG_000276"
     CDS             complement(13213..15444)
                     /gene="pflB"
                     /locus_tag="ACGVJG_000276"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFJ6556264.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(15721..18171)
                     /gene="parC"
                     /locus_tag="ACGVJG_000277"
     CDS             complement(15721..18171)
                     /gene="parC"
                     /locus_tag="ACGVJG_000277"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFJ6556265.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(18183..20228)
                     /gene="parE"
                     /locus_tag="ACGVJG_000278"
     CDS             complement(18183..20228)
                     /gene="parE"
                     /locus_tag="ACGVJG_000278"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFJ6556266.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(20394..20828)
                     /locus_tag="ACGVJG_000279"
     CDS             complement(20394..20828)
                     /locus_tag="ACGVJG_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFJ6556267.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            20955..21593
                     /gene="plsY"
                     /locus_tag="ACGVJG_000280"
     CDS             20955..21593
                     /gene="plsY"
                     /locus_tag="ACGVJG_000280"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFJ6556268.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(21725..22600)
                     /locus_tag="ACGVJG_000281"
     CDS             complement(21725..22600)
                     /locus_tag="ACGVJG_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFJ6556269.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(22682..23473)
                     /gene="codY"
                     /locus_tag="ACGVJG_000282"
     CDS             complement(22682..23473)
                     /gene="codY"
                     /locus_tag="ACGVJG_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFJ6556270.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(23491..24891)
                     /gene="hslU"
                     /locus_tag="ACGVJG_000283"
     CDS             complement(23491..24891)
                     /gene="hslU"
                     /locus_tag="ACGVJG_000283"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFJ6556271.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(24905..25453)
                     /gene="hslV"
                     /locus_tag="ACGVJG_000284"
     CDS             complement(24905..25453)
                     /gene="hslV"
                     /locus_tag="ACGVJG_000284"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFJ6556272.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(25490..26395)
                     /gene="xerC"
                     /locus_tag="ACGVJG_000285"
     CDS             complement(25490..26395)
                     /gene="xerC"
                     /locus_tag="ACGVJG_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFJ6556273.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(26593..27969)
                     /gene="trmFO"
                     /locus_tag="ACGVJG_000286"
     CDS             complement(26593..27969)
                     /gene="trmFO"
                     /locus_tag="ACGVJG_000286"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFJ6556274.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(27938..30016)
                     /gene="topA"
                     /locus_tag="ACGVJG_000287"
     CDS             complement(27938..30016)
                     /gene="topA"
                     /locus_tag="ACGVJG_000287"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFJ6556275.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(30138..30995)
                     /gene="dprA"
                     /locus_tag="ACGVJG_000288"
     CDS             complement(30138..30995)
                     /gene="dprA"
                     /locus_tag="ACGVJG_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFJ6556276.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(31051..31818)
                     /locus_tag="ACGVJG_000289"
     CDS             complement(31051..31818)
                     /locus_tag="ACGVJG_000289"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFJ6556277.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(31811..32671)
                     /gene="ylqF"
                     /locus_tag="ACGVJG_000290"
     CDS             complement(31811..32671)
                     /gene="ylqF"
                     /locus_tag="ACGVJG_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFJ6556278.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            32931..33329
                     /locus_tag="ACGVJG_000291"
     CDS             32931..33329
                     /locus_tag="ACGVJG_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFJ6556279.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            33566..34108
                     /locus_tag="ACGVJG_000292"
     CDS             33566..34108
                     /locus_tag="ACGVJG_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFJ6556280.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(34191..34742)
                     /gene="lepB"
                     /locus_tag="ACGVJG_000293"
     CDS             complement(34191..34742)
                     /gene="lepB"
                     /locus_tag="ACGVJG_000293"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFJ6556281.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(34894..35121)
                     /locus_tag="ACGVJG_000294"
     CDS             complement(34894..35121)
                     /locus_tag="ACGVJG_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFJ6556282.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(35118..35636)
                     /gene="msrA"
                     /locus_tag="ACGVJG_000295"
     CDS             complement(35118..35636)
                     /gene="msrA"
                     /locus_tag="ACGVJG_000295"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFJ6556283.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(35656..36369)
                     /locus_tag="ACGVJG_000296"
     CDS             complement(35656..36369)
                     /locus_tag="ACGVJG_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFJ6556284.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(36372..37268)
                     /locus_tag="ACGVJG_000297"
     CDS             complement(36372..37268)
                     /locus_tag="ACGVJG_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFJ6556285.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(37426..38268)
                     /locus_tag="ACGVJG_000298"
     CDS             complement(37426..38268)
                     /locus_tag="ACGVJG_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFJ6556286.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            38400..39053
                     /locus_tag="ACGVJG_000299"
     CDS             38400..39053
                     /locus_tag="ACGVJG_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFJ6556287.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(39116..39643)
                     /locus_tag="ACGVJG_000300"
     CDS             complement(39116..39643)
                     /locus_tag="ACGVJG_000300"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFJ6556288.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(39663..40610)
                     /locus_tag="ACGVJG_000301"
     CDS             complement(39663..40610)
                     /locus_tag="ACGVJG_000301"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFJ6556289.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(40622..42505)
                     /locus_tag="ACGVJG_000302"
     CDS             complement(40622..42505)
                     /locus_tag="ACGVJG_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFJ6556290.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(42668..44746)
                     /locus_tag="ACGVJG_000303"
     CDS             complement(42668..44746)
                     /locus_tag="ACGVJG_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFJ6556291.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(44759..45034)
                     /locus_tag="ACGVJG_000304"
     CDS             complement(44759..45034)
                     /locus_tag="ACGVJG_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556292.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(45396..45851)
                     /locus_tag="ACGVJG_000305"
     CDS             complement(45396..45851)
                     /locus_tag="ACGVJG_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556293.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(46005..47213)
                     /locus_tag="ACGVJG_000306"
     CDS             complement(46005..47213)
                     /locus_tag="ACGVJG_000306"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFJ6556294.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(47325..47657)
                     /locus_tag="ACGVJG_000307"
     CDS             complement(47325..47657)
                     /locus_tag="ACGVJG_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFJ6556295.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            47805..48680
                     /locus_tag="ACGVJG_000308"
     CDS             47805..48680
                     /locus_tag="ACGVJG_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFJ6556296.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(48762..49343)
                     /locus_tag="ACGVJG_000309"
     CDS             complement(48762..49343)
                     /locus_tag="ACGVJG_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFJ6556297.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(49349..50608)
                     /locus_tag="ACGVJG_000310"
     CDS             complement(49349..50608)
                     /locus_tag="ACGVJG_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFJ6556298.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(50610..51656)
                     /locus_tag="ACGVJG_000311"
     CDS             complement(50610..51656)
                     /locus_tag="ACGVJG_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556299.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(51891..52166)
                     /locus_tag="ACGVJG_000312"
     CDS             complement(51891..52166)
                     /locus_tag="ACGVJG_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFJ6556300.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(52409..53719)
                     /gene="der"
                     /locus_tag="ACGVJG_000313"
     CDS             complement(52409..53719)
                     /gene="der"
                     /locus_tag="ACGVJG_000313"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFJ6556301.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(53901..>54824)
                     /gene="rpsA"
                     /locus_tag="ACGVJG_000314"
     CDS             complement(53901..>54824)
                     /gene="rpsA"
                     /locus_tag="ACGVJG_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFJ6556302.1"
                     /translation="DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPAS
                     MVEDHFVADFSDYKGKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEG
                     DVLSGRVARLTDFGAFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINP
                     EQERVSLSIKDTQPGPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQ
                     ISHKHIATPHEVLHEGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYEL
                     PEENTGFTMGDILGDALKEEVEEKATDDSEK"
BASE COUNT        15000 a        11482 c         9340 g        19002 t
ORIGIN      
        1 cacacgcaaa aatttttgga ttgctcgttt gcagataatc attgactgca attccgtgtt
       61 tatcaaagac gatattcgct tgctcaagtg ctaaagattc tgtattcggc tgtctcccag
      121 tcgcacaaaa aatcatatct gctactaatt ccgtctcttt tccgactaaa cgatagcgag
      181 taccttctga aatgatcttt tgggtttcaa caccaaaagc aaactgaatg ccagatgctt
      241 ccatttgatg gacggcttcc tctaccaaag acgcctcaaa ttcttttaat ggccgttgat
      301 tatgatggac aatggttact ttgctccctg cagcattggc aatcgtagct agctcaaaag
      361 caatataacc accaccgata aaaatgattt ctttaggcag tacaggcaat gataaaaagt
      421 ctgcactagt tttcaagtat tcctttccct ccactggtaa gatcgtaggt ctttgcccgg
      481 ttgccaaaac taagtaatcc gcatggaaga cttcttcgtt gacttcaata gatgaatcat
      541 ccaaaaaacg agcggttcct gaaagatgat cgatttcagc ttctgctaat tgtttttttc
      601 ggctttctgg gactggatcc gtaaatgttc gtttgaattt ttgaagttct tcccagttgg
      661 cagttgggat ctcgttaaaa ccttttccac tcaattgttt tacccgatta cgtgcctcta
      721 ctgcacttaa caagactttt ttaggatcac atccacggtt aggacaagtc ccgccccata
      781 gatcttcttc gaccactagt actgttttac ctgcttcttt caatccataa gcagcactta
      841 agccgctcac acctgaaccg atgatgattg catcatatgt tttcatttcc ttcctcctct
      901 cgacttaaaa aagcaaagat ttctttactt gtcagcgacc caatacttct ttgcttttct
      961 actatcacaa tgcttggata caccgaaaga agcgaatata cttctttcat aggcgtatca
     1021 aagcaaactt gttgacgagc attttctggt atttcattta agaaatatcc attgacgact
     1081 aaatcttcta ctcgccactc tttcgtctgt ttcggttcat caaagaaatg ccgaacgtag
     1141 tcgtttttag gattcatcaa taactgctct ggtgtgtcac attggataat ttctccatcc
     1201 ttcataaatg caatacgatc acctagcttg attgcttctt ccatattgtg cgtaacgaaa
     1261 acgatggtcg tgcccaattc ttcgtgtaag gacaaaacca attcttgtaa tgagtttctt
     1321 gaaatcggat ctaatgcgct aaatggctcg tccatcaaaa tcacatctgg gcttgcagca
     1381 atagctcgga tgatcccgat ccgttgctgt tctcctccag acaactctct aggcatacga
     1441 tctcgataaa tatcaggaga taagccaact ttttgaagaa gttcatctac acggtctgct
     1501 cttttctgct tttcccaacc taacatttca gggatgactt caatattttg cttgactgtc
     1561 atggtcggga acaaagcgat ttgctgcagc acatatccca tattccaccg cattttctgc
     1621 agattatatt catttaacga ttttcctttg aagtagatgt ctcctgctga tggtttagat
     1681 aatccattga tcattttcaa agtagttgtt tttccactac cggaaggacc aaccaaaaca
     1741 aagatttctc cttttttgat tgccagattt aggtcatcga tgacatactt tccatcatac
     1801 tttttttgca catgtttgaa ctcgattaat ttagtcactg atcttcctcc tttactaatc
     1861 cttcattttt caggaagatt cgtgctactt ctgtcggctt tttcttttcg acattgactt
     1921 ggtaattcat atcaatcatt tgatcctctg ttatttttcc gcttaattta ttcagtgctt
     1981 taacgatttc aggatgttct ttagcaaaat cttttttcat cagcggtgcg ccttgataag
     2041 ctgggaataa ccctcgatca tcttctagag tcactagatc gtactccttg agctggctat
     2101 ctgtggaata ggcatccaca atattgacat ctccattatt gattgcttgg taacgcaaac
     2161 ttggctccat gctttgtaca cttgaaaaat tcaagccata tgcttcttgg attcctttat
     2221 atccatctga acgatcaata aattccagtg tgaatcccgc tttgatctga tcagagatag
     2281 ctgccaaatc ggaaatcttt ttaagttgat gttcgttcgc aaaagatctc ttgactgcca
     2341 aggcatacgt attttggtaa gccattggtt ttaataatgt catatcgaat tgcttattca
     2401 ataattcttc tgcttcgtca tatgtttttt cttttgtcaa atcagcactt ttcattgctt
     2461 caggtacctt caccaagctt tctaggacag tacctgtgaa ctcaggatag atatcgattt
     2521 gaccttgatc cagtgcattg aacaaaaaac tcgtcttgcc aaaattcggt tttaattcaa
     2581 cttctgtttt tgtgttgtct tcaatcaatt ctttatacat ttggatcagg atttcaggtt
     2641 ccgctcctaa ttttccagca atcgtgatgg tttcattttt aaacaaggca gactgatata
     2701 aagaaacgcc gccaaataaa cacaccactg ctgctaaagt aatgatggaa tatcgaggtt
     2761 tcttatgctg caaccagcga atcagtccgc tgaatataat tgcaagcaag gcagaactga
     2821 tcgcaccaat caatgtcaaa acaggtgtat ttcggtcaat acctaacaaa ataaatgtcc
     2881 ctaatccacc tgctccgatc aatgctgcca aagtagccgt accgatgatt aaaactaagg
     2941 cagttcggat acctgagata atgacaggta atgcgatggg aagctctact ttcagcaagc
     3001 gtctcattct ggacatacca aatgctaccg ctgcttcttc aatagacggg tcaatttcag
     3061 ctagaccaat atacgtgttt tggaaaatcg gaagcaaagc gtaaataaca agtgcaatca
     3121 atgctggaac agttccgatc cctacaaatg ggataagtaa acctaataag gcaagtgaag
     3181 gaatcgtttg gagcacaccc gtgatctgca ataagacttc tgccattttt ttatgattgg
     3241 ctgcccaaat cgctaaagga atagcaatca gtgctgcaat cagcaaagaa atgagagaaa
     3301 tggacagatg ttgaaatgca gcttccagca actcgccttt tctttcactt aatgtttgta
     3361 taaaattatt catgcgacct tcctttctgt tattctttaa gtataaacat ttctttcctt
     3421 tttcccaact aacttgtctt gccttaacct tacagcacga acaaaaaaaa gaaaaaaata
     3481 cctctaaata tgctgacaaa aaaagaatcc aagcaacaaa gtcggatcaa ctttttggtt
     3541 ctcgtttttt cactacgaat accataaaaa tctatacgaa attgtcactt ggaatcagct
     3601 atatcttttt tacacacaca acttttattt taataaaaag aaacagccaa taaagtagaa
     3661 atctcgtggg ggagaacacc tttattggcc tgttcaacga atattatact gtatcatgac
     3721 acttttttgc caaaaatgta aaaaatggtt gttttgacat cataaacgtc ttgttttctt
     3781 atttttactt atatcaccac tttattttct atctctttca ctccataaag aggaatgagt
     3841 tcttcttttg actgataata aacagctttc tcacggttct tcatcaaaaa aagaaagatg
     3901 tctttcatag caagtggtgc tttgccgtgt tcaaataaaa agataaattg atttgcttta
     3961 tttatctgga ccattgtcac aggcaccagt ttttgttctt gagccaaaaa aactccttga
     4021 tactctgttt ttttgggtga ttttgtaaga attgtcaata agtctgctgc tcgcattttt
     4081 ccactcctta tttgataaat aaaaagaaac agccaaaaat aatttttttt cattttcctc
     4141 ttgaagtaag agtataagtc agtataataa agaagttgct gaaaattttc aaaagaaaag
     4201 aggctagaaa tgaaaaaatt aaatttattt acagaattgt gcggaatcat tttaggcgca
     4261 ggaatctacg gattagctgt gacaggaatc aatattccat ctaagctggc agatggtggg
     4321 gttactggta ttgctttgct tttgcataat ctttttggat tcgctccttc tgtcacttca
     4381 ctgcttttta atttaccttt attgcttatc tcattgtttc tttttggaaa acacgctttt
     4441 ctccggacga tcgtcggaac atttgcgcta gttttctttt tacatttatg ggagagcctt
     4501 catatacatt ttgctgtaga tagtctactg atcaatagtt tgatgacagg tattctttct
     4561 ggcattggtt gcggtctcgt ctttcgattc ggcggaagca caggcggaac agatatcatt
     4621 tatcaagcca ttgaaaaata ttttcatatt aagattggca aaagtctttt cttgattact
     4681 tttggtattt tagttgtctc cttgctttat ttggatctca cacattttct gtatacatta
     4741 cttagctgtt ctattctatc ttatacattg aataaagtga agtatctgga aattggatct
     4801 gttttgcccc aattggaagc aaaacagact acagaaacat ttgaaccact tgatcctctt
     4861 gaacagttag agcactgatt cttatttttc actactttat ttaatttaca gaaatatcat
     4921 tatgtaaact aaaatgatat tattcagtaa aatcttcttt ataaaaaaat aaactatttt
     4981 tgcaaacgat ctgcgaaaat agtttgtttt tttattttgc cattattttc ttacttattg
     5041 ggagaaaaaa ttcgcttaaa ctcaaaaaaa tttgaggata cgatataaac tttcggatag
     5101 agtataaaat aaggaaacgc cgcagcgttt cctttccaac tgtagccgaa tataaatttg
     5161 tatatcactt atttaaaata gctattttct cccaaagagt ttccttgatc atcgatcaat
     5221 ccttgtaatg agcgttctaa gataccatgg ctttttttcg attttttttc ttctgactgc
     5281 tttgtttcct ctaacgtttt tgttcgattg aatgttcgta cagacggctc accatttctt
     5341 ttttcttgat acggcgtcgg ttctgtatcg aacaacagat aagagtcatc cgtttttttc
     5401 atcgattcga ttaaatgatc ctcagaaatt ttgctttctc tagggtctgg gataatcgat
     5461 gcgggtacat atttaggaac aaaataagaa cgctcccgca ccttcgtgtc aacagatgcg
     5521 tttcgtgtag tatgcttgga ctctgtcctt gtttcacttt gtctgacaga acgagaggaa
     5581 tcaccaaaaa gtttttttcg tcctgtcgat gtgatttctg attggtaatg gccggtataa
     5641 tccggtaatt ttcctttatg cttttccaaa ttttccttac gcggagctga cttcaatcca
     5701 tttgttgcac gacgcttgcg acggattttc tcggtgttcg tgttcgtgaa atgaaattct
     5761 ttcatctgtt caaacgggga ctgattctgt tcagtcaaaa gatcttcttg tccgtcaaat
     5821 aatactcgct tcttgtcgtt ggttaatttt acgcctaact catcatcgta cgcaggaaaa
     5881 cggaaatgtt ttcgtctgat ttctttcatt caaaccatcc ttctcaaaaa tggaactttt
     5941 ctcaataata gcacagattc attgttttca gtagcaatgc gctgattatt ttaagaaaat
     6001 agcgatgaat ttcataaata tatatatagt aataagactt aattcgttgt ctctctgata
     6061 actagttcag gaaaaaaacg acgataacct tgactcgccc ctttttcttc tatttttttt
     6121 aacaacattt ctccacatac ttcacccata cgtataatat cttgcttgat cgttgtcaat
     6181 ttaggtgaac tgatttggtc taaaaatacc ccatcaaatc caatcacacc aaattcttcg
     6241 ggcaagtgtc ctccagcatt tataatccct cttactgctc ctagtgctag ccgatctgaa
     6301 ctacatacaa aacaagtttt atctgtaaaa tctgcccagt tttctgtcat atatctttct
     6361 acttctgtcg aatgatttgg aaaccgcaag attttactag gaagatcaac ttccttcatc
     6421 acagataaat aaccagtttc ccttgaaatt tcaaaaggct cgtccacttc gataccgata
     6481 taaataatcg tttgataatc tctttctaag gcatattgcg ttgcttttgc tgtaccatat
     6541 tcattatcgc tgtctacata atcatatcct tcagaatttt ccccaaacaa gacaactggt
     6601 ttttccaaac ggtcgatcca atcgaagtct ttttttctga cacctgtgat gatgtatcca
     6661 tcacaagaac cgacgtcgaa ggcatcattt gtaactaatt ggagagaata atgcgcttta
     6721 ccgatccgtt tagcgattcc catcagcaaa ttcatgtaat agggctcagt tgtgtcgatt
     6781 tcttccagta taaataattt gatgatctgc gaccgattcg tgacaagtgc ttttgcagca
     6841 atatttggac ggtaatccag ttcagccatt gctgagaaaa ctaactcttt caattcgtca
     6901 gtcacctgtt ccggatgatt gatgactcga gagaccgtca tttttgaaac attggcacgt
     6961 tttgcaacat ctgctaaagt gggcatgctc tcttcctttc taacatgttg tcattcttac
     7021 tatcaattat accaaatttt cctaacagag tttattatag cccaaaataa aaaacggaaa
     7081 atcctctatc gtcatgagtg attttccgtt tcaattattt atactttttt atcaaatttt
     7141 ttgctaaaaa agtggaagct tcttccatta gtggtatttg taccatatgt cgctgtgttt
     7201 ttgaggaacg aaaaacaatt tcttttccgt aagacttatg ccgatttttt tctacaaatc
     7261 gttccgtttg tatataaggg attttttcgt cagcttgtcc atgccaaaag aacagaggac
     7321 gtccagctaa cttttccggc tgcaatgaca aatcatagct cttaacccat gaaatcaaat
     7381 caaaatagtc ttttggtacg acataattat attccgatgc gcgccttacg atttcttttc
     7441 catattccaa aggtgcaggg gaacccatca aacacacgcc cgctactata tgaggatgtt
     7501 tagccaacaa ggcgcatgtt gtcattcccc ccatcgaaac cccgccaact gcaagctgat
     7561 catgtagaag acctcttttt tctaaatgct tgataagtgt gtcaaattca aacagatttc
     7621 cgtagatact atcccaaaat gtataggatg gaatagaaga aaccggctgt ttgcgctccc
     7681 catgattcat cgcatctggc aggatcacac gaatgccctg ctgcgcaatt tttcttcctt
     7741 gcgtcaaaac taactcctta cttgtctgcc atccgtgata atagatgaca acgggaatac
     7801 gttcctctct taattctttc agaacaactt ctaacactgg aatatgatgg acagaacgtt
     7861 tcaatataat tggattcaat tgttactcac ctattttcat taaatttcct gatcgccata
     7921 gcaaccatgc tccaacagtt gtctggaaca ggttcagtgt accaaataat ataaatgcag
     7981 gactgaatgg ctcgggaaat aagcacagag aacaaagtcc caccactcct aatgctagcg
     8041 aaaggagtgg ataaatagaa aaattcaaaa aactggcaac gctaccgaaa atattaccaa
     8101 taaacagtat caaaaagaaa aaaacgctta aaattaaacc atctgtcagc gacatctgaa
     8161 aaacaatgtt ctctacctca attggctgtt gaatcgaata gacacctaag tatatactga
     8221 gaaggagact tagcacgtgg ctggcacaaa acacacgatt gaccgttttc attttaatca
     8281 cctaacctat ctttataatt ctattataat cattttatca tttttttgta tgtataaagt
     8341 ttcaattttt cttcacttac gatttttttt gatattgttg ttatataaat gaaaggagtt
     8401 ttacaaatga ttgatccaaa aatgttgggg atgatcttcc tcattaattt tgcttatatc
     8461 actctgaaca cgttacgttt catgttgaca atgaaaggct atcgactgat tgctccactt
     8521 gtcagtatgg tcgagatcac gatttatatc gtcggcttat cccttgtttt agatcgttta
     8581 gataatccac tgaatttatt tgtctatgcg ttaggttacg cagtaggtat cagtgtcgga
     8641 attttgattg aagacaaatt ggcattagga tacatcatgg tcacaacgat tttgccagca
     8701 aatagcgcag aagataaaag tttgccgggt atactgagag aacacggtta tggtgtgaca
     8761 cagacttctg caatggggct ggaaggtgag cggctagttc tcgaaatact ttctcctaga
     8821 aaaagcgaac gtgaactgta tcgcctgatc aaagaaatcg aagagcgtgc ttttatcatt
     8881 tcttatgagc caaaatatat ttcaggtggt ttctggacta aaaaagtgag aaaacgacaa
     8941 ggaaaataac tcacaaactt aaaaactgtc taatatctaa attaaatttt aaatgttaga
     9001 cagtttttat ttattatttt gattaaaaaa catcgacata tactcatcaa tttgttatta
     9061 tactttatat acttatcaat ttaatttgga gatattatgg aaattcaacg tagaaccact
     9121 aattttttac atttaggctc tttttttgca actttgacga tcttattttt tctatgttat
     9181 tcatacctta agacctcaca tgcgccttct agtgcattag gctggttgct tttggatcat
     9241 tctgatatca cagaagccga agaaaatccg ttttttttga ttttgtcttc tctttgcggc
     9301 ttactgtttt ctgtttattt cggtgcctat ttttttcatc gacatcctgg tacgttaaac
     9361 gaagcttcaa gcgtattgat tgcatttaaa acgttttctt ttttagcaat tcttttttat
     9421 tcgtttaatc acaatacgct tttcttcttt atcatgaatg gtttagtcgt gattttatcc
     9481 atttttactt attttatttt ttcactcatt tttagccaat tgaaatgtcc gctcttcttt
     9541 cgattagttc ctgtcacctg ttttatttac agcttgtttt ccttttttgt ccttgcttta
     9601 atgccactga ccaacccttc gacgatccaa gtcgcaggaa atctgttgga tatgctcttc
     9661 atcgtcagtc taggtgtatt tatgtggcaa agaaaaaaca aacaaaataa aaagcatttt
     9721 gaagcctgac tattacgact tcataaatgc ttttttagtt ttaaacactt caatctaaaa
     9781 gaataaccca agattggata aattattaat gactcgccat tcgtttctta gtgattctgg
     9841 attatcaaat ggacttccta atctaatcag ctagttcttc ccataaaatg ccatacttga
     9901 ctgaatcata atattgatta tttaaaaatc taacttttct tatcactact tcctttgtca
     9961 ttccacattt ctcaccattt ttttgcattc ctttgtttcc agaccatgta gtgaatctaa
    10021 taggtggtag ataggcaaaa tactcaaata attcttttaa ccataaagga agagaaaggc
    10081 tcctcctgca agagtcagta taatttttgc ttactgctct cttaggtttt actcactaaa
    10141 actgttccat attctttgat ataagaaaat gcatgcggta taaatttctt gctcagctca
    10201 gaaaagattt gtcgtttaag aaaggccatt tcctccattg tcaatgaagg atccaatgtt
    10261 ggtaattcaa tctcaaaatt gtttttttcg acaataggta attctaatgt gaatgattct
    10321 tcttccttta tagtattcgt cacaagtaga gactgatgga aaatactagg ccggcgattt
    10381 ttatatttat tcttacgtat cctgtcaacg ataacttcta tttgatcaaa aaagtacgat
    10441 tcatttacgt ctccaataag ttcggctaat tctttgaatg ccttcgttac aggaagctga
    10501 ttcttctgta caaaaaagta attagccaaa gtaagcggga agtgttctat agaggaaagt
    10561 gtgataaaac ggtacttctc atttcctgca gttgtcctca gcaattctgt ctctcggctg
    10621 tttcttactc cataaaaata cgcttctttc cgttcatctt cgaaacaata cgcccaaata
    10681 acttctccca tcgctaattt ttgttcatca acagagagtc tcttcaattg ttcagctatg
    10741 gtttcagcag ccgaagtggc ttgttgcaag taatctttag gatcaaatat tggaaagttc
    10801 aaatgatatc ttcgttcttc cctcttgatt aatcctgcat cgatcaataa atcaagaaaa
    10861 tgggaaaatt tattttgcga aaattctttt ttcaattctc ttagaatcgt ttcttgttct
    10921 cgatgttcat atagataatc ggtcaatgaa gaaaaaagcg gatcatcggt cagtttttga
    10981 taaaagtttt ccttattttt tgaataatag ttcatatatg ttctcttttc tatccgtttt
    11041 attttactta aaaggctttt acgtaaaaag gaggaagaac aagagtcata attgactgtt
    11101 attctcctcc atttcttcgg cctttagtat gttctatgtt tctttttata tcaatcgctt
    11161 atccattcat tttgaatgct tcatactacc atttgattag ctaaatacag ccgtcaattg
    11221 aggtacgact tgttttttac gtgaaacgac acctttaagc aaagcacggt tgttggtcaa
    11281 agttgtttgg aaagcttctt ctactttttc ttttccttta ccagcaacaa gtaattctga
    11341 atcactgtct aagatgtttg tgacaactaa tacgaacaga tcgtagccat ttgctttcat
    11401 ttcatcttcc attgctgctt ctagctctgc ttgtcgatcc atcacttcat taaggtccac
    11461 tgtattgact tgagcgatac gaacgttgga actgcccatt gggaaacttt ttgcatccaa
    11521 atcaagaaga accgcagctg atttatcact tagatttgtt cctgctttca acatttcaag
    11581 tccataagtg tttagatcga cttcagcgat tgctgcaagt tcttttgcag ctgcttcatc
    11641 ttcaggcgta catgttggtg atttgaacaa caatgtatct gaaatgatag ctgagagcat
    11701 gattcctgcg atttctttag gaattgttac ttggttttct ttatatagtt ttaaaataat
    11761 cgtacttgta catccaactg gttctgcacg atagtacaat ggattagctg tttcaaagtt
    11821 tgcaatacga tggtggtcta ctacagctaa aatatttact tcgctgatat ctgaaatact
    11881 ttgttggaat tcattatgat ccacaagcat gacatctgca ttttctgctt tagctgactc
    11941 tactactcgt ggtgcagata gctgaaagtg gtctaatgcg tattgtgttt cttcattagg
    12001 tgtacctaga gcaactgctt ctgtttcttt acctaattca ttttgcaagt gtgaaaacgc
    12061 aatggctgcc ccgattgcat ctgtatctgg attttggtga ccaaaaacta aaatttttga
    12121 catgtcattc gctcctttgt gatttctcct atttaataat agcaaaaaaa cggaaagaag
    12181 caatacttat cttctttccg atgcgaccta tcgcgttaaa tattttgtat actcttctgt
    12241 atgaagcaat cgtttagcat tttcgacacg ttcttttgta ggtggttcga tcccttctaa
    12301 tggatatttc aaaccaagtg tttcccactt gtattttccc atcgtatgat aaggaagaat
    12361 ctctactctg tctacatttg acaaggtttg aataaagttg tctagtcgga tcaaatattc
    12421 atcataatca cttcgctcag gaactagtac atggcgaatc caaaccggtt tgttgatttt
    12481 tgacaaatat tgagccattt ctaaaatatt ttcattgtcc caatgcgtta gttttttatg
    12541 ctcttcatta tcgatgtgtt tgatgtcaaa taacagtaaa tctgtatact tcatcaattc
    12601 ttgaaaacgt gaaaagaaag gttcttcata cgtaaaaggt tttccgcatg tatccaatgt
    12661 agtatgcatc ttccgctctt ttgctttttt aaatagttcg atcaaaaaat cgatctgcaa
    12721 tagaggttct ccaccactaa cagtaatacc acctttatct ccccagtaag gtctgaagcg
    12781 ttctgcttta tctaaaagtt catctgctgt gtattctttc cctccgccaa tattccaagt
    12841 atctggattg tgacaaaatt ggcaacgcat gcggcatcct tgcataaaca cgacaaagcg
    12901 cagtccaggt ccgtctactg aaccgaaagt ttcgatagaa tgaacatagc cgattgtttt
    12961 ttcttccatt taagttcaca tcttttcttt gatttcattt taagtacaag ttagctgtcg
    13021 aagcgaagct ttctgaacaa aatttttcgc aattgccgct tcgttatgtg taaaatttac
    13081 agggacggaa catacaagtc ctgcccctgt aaatcgtttc tttcaaataa gcatactgat
    13141 tattcgattt tgatgcgatg gctattcaac gtcctttcga cgctaagtcg aaaaactttt
    13201 ttcacgaact ttttacattc tgtcgtgtga agttctcatc aagacatcta attgttgttc
    13261 tcttgtcaag tcacggaatt taactgcata accagatacc cggatcgtca agttcggata
    13321 tttcattgga ttttccattg catcacgcaa taaatcatta ctgaatacat tgacattcaa
    13381 atgataagct ccttttgaga agtatccatc taagacatta cgcaagtttt cgattcttgt
    13441 atcttgttct tttccaagac ctgaaggatt gattgtttgg gtatttgaaa ttccatccaa
    13501 tgcacagccg taattcattt tagctgttga gttcaggcta gctaataacc cactttgttc
    13561 cgcattgtaa cttggatttg ctcctggcgc aagtggtttt ccagcttcac ggccatcagg
    13621 agtatttcct gtatttttac cgtaaacaac atttgaagta atcgtcaata atgaagttgt
    13681 tggttttgag ttgcggtaag ttttatgacg tttgatttgt cccatgaagt attccaagat
    13741 ccatgaaact aattcatctg cacggtcatc atcatttccg tatttaggga agtcgccatc
    13801 cacttcaaaa tcaactgcta ttcctttttc attacggatt gcttttactt ttgcatattt
    13861 gatcgctgac aagctgtctg ccgcatgaga gatcccagca atacctgtag caaatgtgcg
    13921 ttgcaagtca gaatccatca aagccagctg agctgcttca taagaatatt tatcatgcat
    13981 ataatgaatg atgttcaacg tattgacata tagttctgct aaccaatcca tcatattcat
    14041 gaagttatgt ttcacttctt caaagtctag tatttctgat gtgattggac gatattctgg
    14101 tccaacttgg gcgccagtca cttcatcgac tccgccattg atggcatata atagtgtttt
    14161 agccaagtta gcacgagcgc caaagaattg catgtctttt ccgacaactg ttcctgatac
    14221 acagcaagca atcgcacaat catcagaacc ccattgttca cgaagctgat tatcattttc
    14281 gaattgaatc gaagaacttt cgatagcgat tctgctagca tatgttctga atccttctgg
    14341 aaggtgttca gaatacaaaa cagtcaagtt tggttctggt gaaggtccca tatttgttaa
    14401 tgtatgaagc agacggaaat cattctttgt aactaatgaa cgaccatcta atcccatacc
    14461 tgcaatggat aaagtagccc agatagggaa tcctgagaat aattggttat attcaggtgt
    14521 acgcgcgaat ttgaccatac gtaatttcat tactaagtgg tcgatcaatt cttgtgcttc
    14581 tttttctgtg attactccat tatccaaatc acgttggata tagatatcaa ggaatgctga
    14641 gatacgtccg attgacatcg cagcaccatt ttgtgatttg attgctgcta aatatccgaa
    14701 gtatagccat tggacagctt cttttgcatt tgcagctggt ttagaaatat catagccata
    14761 tttcgcagcc atttctttca tttgcgctaa tgcacgatat tgttcagtga tttcttcacg
    14821 taaacgaatc acttcttcac tcattgtatg ataaccagtc atatcatgat cttttttctt
    14881 ttgttccatc aaatagtcga ttccatacaa tgccacacga cgatagtcac cgatgatacg
    14941 tccgcgaccg taagcgtctg gaagccctgt aatgattttg tttttacgag cagcacgcat
    15001 ttcagcagta taagcgtcaa atacaccttg attatgtgtt ttgcgccagt cagtgaaaat
    15061 atgtgtcaat tcatcgtttg gtgtatagcc attgcttttt agcgcattgt tagccatatt
    15121 gattccgccg aacggcatga atgcttgttt caaaggtttg tctgtctgta gaccgacgat
    15181 tttttccaaa tctttatcca aatagcctgc atcgtgtgaa gtaatagttg atacgatttc
    15241 tgtgtccata tcatagacac cgttacgctc gaattggata tcatttaact tttgcaattc
    15301 atcccataat ttttctgttg cttctgttgg gccttccaaa aagctgtcat caccgcgata
    15361 ttcagtgtag ttattttgaa taaagtctct agtgtctacg ccttctttcc atttggtacc
    15421 tttaaatcca ttccattgct ccattgtatg gacctcctaa atttctgttt tatgtaacag
    15481 tttttacgtg attagtataa cacagcaccc tatctttgca agggttttct tgttttttat
    15541 ttaacaattt ttatgtttta tttacatata tttaaatcaa cgttgacata ccaacattta
    15601 agcattgtga acactttcat ctttttttgc aaacgtataa ggatttatta aaaatgaatg
    15661 aaaaagagac gccacctgta ttaatacaga tggcgtctct tcattaaaat actacaacag
    15721 ttacgactcg atgaaaatct caggagcatc gataacttcg atgacttctc cacctttttt
    15781 ctcatccatc gcaaatgatc cattcgatgt tcgatcacct atagggaaat cagttgggtc
    15841 aatcgttaac tgctgccctt tttgattcat caaggttatt tcttttgatc ttccttccga
    15901 catatagata atacggtgcg gatttttttt cagctcacgc atgaccgtca ctccccgctt
    15961 cgcacgtgaa gtttggctga tttcttgagc aagcatgcgt ttaacagctc ctcgctgagt
    16021 aacgattacg atcggcgtat caccttctgc taacacaagc agcccgttga taatcctatc
    16081 tccatctttc aaattcatag acttcactcc agctgccttt gctccaacaa cgggaacatc
    16141 tgttaaagga tagcgcaaac ccattccttg atgcgttact aagaaaacat ctaagcctgg
    16201 ctgatcttct ttcagataca cagcaatcag ttcatcttct tccgccttta gcttcataca
    16261 gctgagcgga cggctcttat aggtgcgcca tggttcgaac tctgtcattc gtgtttgctt
    16321 gatcaatcca ttcttgctga tgaatacaaa tgttttctct gcttgaattt tttgatatgg
    16381 gaaaacagct aaaatcgatt cgtctacaga cagattggag attgtttgag aaatgtgctc
    16441 accagcatct ttccatttca aatcgggtag ttcatggaca gggcggtaga tcacatttgc
    16501 tttatttgtg atcagaagaa catggtccaa tgtactcaat tcacctgagt atagcaagta
    16561 gtcgccttct cgcatcccga tttcttctgg tttggaagca ctataagaac gaaggcttgt
    16621 tcgcttcaca tagccttctc gcgtcacact gacaattacg tcttcttgtg ccactaagac
    16681 ttgcgtatca attttgattt cttcgatttc gtcttcgatt gtcgttagac gagcggaaga
    16741 ataatttttt ttcacttctc gcaattcttt tttcataaca gagaaaagct ctttgtcatt
    16801 tgaaaggatt ttattcaact ctgtgatttg cgcaatcaag ctttcggatt ccttttgaag
    16861 ctctgtgata tccgtgttcg tcaaacggta taattgcaat gtgacaattg cttctgcttg
    16921 ttcttctgta aattgaaaga cgtcgactaa attctttttg gcgtcttttt tgtctttact
    16981 ttcacgaatc gttgcgatta cttcatccag aatcgataaa gctttcatca atccttctac
    17041 gatatgctgt cttttttgag ctttggctaa atcgaattga ctacgttttg ttatgacttg
    17101 ctttcgatgc tcgatataac ttcttaaaat gtctttcagt ccaacttgat gcggagtcat
    17161 atggtcgata gcgaccatat tgaaattgta attgatctgt agctcagtgt ttttgaacaa
    17221 ataattcaaa attccttgag cattggcgtc ttttttcaat tcgacgacaa tttgcagacc
    17281 tgttctatca ctttcatctc gtacttcagc tattccatcg attttcttat tcaaacggat
    17341 ctcatccatt tttttcacca aggtcgcttt atttacttca tatgggattt ctgtgatcac
    17401 gatttgctgt ttattaccct tcattgcctc tatctttgtt ttcgaacgaa gaataacttt
    17461 tcctcgtcct gtttcataag cttttttgat ttcatctttt ccttgcaaga tccctcctgt
    17521 tggaaagtca ggtccaggaa tatattccat caatttttca aggctcgctt gtggatgatc
    17581 gatcatatag attgtgccat cgatcacttc agctaagtta tgagtcggga tttctgttgc
    17641 atatccagca gatatacctg tcgatccatt gacgagaagg tttgggtatc gagctggaag
    17701 gactgttggt tctttttctg tatcatcaaa attccacacg agatcgactg tttctttctc
    17761 gatatctgct agcatctctc cacttaactt ggataatctg gcttcagtat aacgcatcgc
    17821 agcaggtgga tcaccgtcca tactcccgtt attcccgtgc atttcgatca gtacttcccg
    17881 caacttccag tcttggctca tgcggaccat cgcttcataa atactgatgt caccatgagg
    17941 atgataattc cccatgatat ttccgacaga ttttgctgat ttacggaatc ctttgtcata
    18001 cgtattgcca tctttgttca tggcaaataa tatccggcgt tgcactggct tcaatccatc
    18061 acggatatca ggcagtgcac gttcttggat aatatatttg gaataacgtc cgaagcggtc
    18121 acccatcact tcttctaaag ttaattcttg tacttcttgt cgattttcca tcaggtgacc
    18181 tcctattcta cttcaaataa ctgattgttt tcatttttat ctgctcgttc ttcatctaat
    18241 aaatcattag aaactgaagg agagatttca gtatcctctt ttttatctaa gatactgcca
    18301 tcttcttcga gcgtaaattg cacgtgattt tcgatccatt ttcttcgagg ttctactttg
    18361 tcacccatca gtgtggttac tcgacgttca gcttgcgcag catcatcgat acggacgcgg
    18421 atcaacgtac gggaagtcgg atccatcgtt gtttcccaca attgttccgc attcatctct
    18481 ccaagtcctt tatatcgctg cagcatatat cctttgccga cttttttaat cattgctgcc
    18541 aattcgtcat ctgtccacgc atattcaatg acttgcttct ttccttgtcc tttggatact
    18601 ttatataaag gcggtaacgc gatatatact ttcccagctt caatcagcgg tttcatataa
    18661 cgataaaaga aagtaagcag taatacttga atatgggcgc cgtctgtatc cgcatcggtc
    18721 atgatgatta ctttgtcgta attacagtct tcgatagaaa actcaggacc gactcctgca
    18781 ccaatcgtat agatcatcgt attgatttct tcatttttca agatatcttg cattttagct
    18841 ttttcggtat tgatgacttt tcctcgtaaa ggtagaattg cttggaactt tctgtctctt
    18901 ccttgtttag cagatccgcc agcagaatcc ccttcgacta aatacaattc atttttctta
    18961 gggtttcggg attgtgcggg tgtcaatttt cctgaaagaa gagattcgcc ttttttacgc
    19021 ttcttcccat ttcggctttc ttctctggct ttacgtgctg cttctctcgc ttctctggct
    19081 ttgattgctt tacggaccaa catctggctc atttcgctat tttcctgcaa ataaaagccc
    19141 atttgttcac tgatgacgtt atctactacg gtacgagcaa ctggcgtacc aagtttttct
    19201 tttgtttgcc cttcaaattg cagaagattt tcaggtacac ggatagaaag tacagcagct
    19261 aacccttctc tgaaatcact accttccaga ttcttgtccc gttctttcag caaaccaact
    19321 tttctcgcat attcattata agatttcgtc atcgccgctt tcataccagc ttcatgggtt
    19381 cccccatctt ttgtccgaac gttattgaca aaagataaaa cattttctga ataaccgtca
    19441 ttgtattgat aagcgacctc tacttcgata ccttcttttt ctcctgaaaa ataaacaact
    19501 ggtgtcaatg tatctttttc ttcattcaga taatcaacaa attctttgat tccttcttcg
    19561 taatgaaaaa tttccttgac tggttcttct ccacgaagat cagaaagaga gattttaacg
    19621 ccttttagaa gaaaggcaga ttctcttaac cgttcggcta aaatttcata tgaaaatttc
    19681 gttgtactga atattgtatc atctggtaaa aaatggacag tcgtcccgtt tgcttttttt
    19741 gtttttccta tctttttcaa agtaccgtca ggtttaccgc cgtttttaaa tttctgctga
    19801 tactctacgc catctctaac gatcgttaca attagccatt tcgataaggc atttaccaca
    19861 ctggctccta caccgtgcag tccaccagaa gttttgtacc ctccttgacc aaactttccg
    19921 ccagcatgca gcacagtaaa aatcacttca actgttggta ttccggaagc atgcattcct
    19981 acaggcatcc cacgtccgct atctgcaaca gttatactat tgtcttcatg gatggtcaca
    20041 tcaatctcac tgccataacc tgataatgct tcgtcaactg cattatcgac gatttcatag
    20101 actaaatgat gcaagcctcg gctgtcagtg gatccaatgt acatccctgg tctttttcta
    20161 actgcctcta atccttccaa tacttggatg gacgagtcat tgtattcgtt gtttactttt
    20221 tttgccaagg caaacgctcc ttcaataata ctttgcggca aaaccgcacc acgattatca
    20281 tactagaaaa ggtcgtgaaa aaaaagaggg tagacgagaa atcaatttta attatctcct
    20341 aaccgctttt tcgacattcc cctctatcga tcatctcttc tttgtggtca tttttatttt
    20401 tggttttctt tcaacttacc aagttcaatc ttaatacaac gatccattac gatatctgta
    20461 cgtcctgctt ttcttagcat ctcagctgct tctttgtttt caatccctaa ttgtgcccag
    20521 aacactttag catcagtttt caaaaaatca cctgctattt caggtaaaaa ttcactccgt
    20581 ctaaatacat ctacgatatc gattggatca tcgatttctg tcaaattcgc atagactttc
    20641 tcgcctagaa tttcttcacc agccaataat ggatttactg gtatgatttt atatccattg
    20701 tcttgcaata cctgtgcaac catgtaactt gttcgttctg gtttattgct caagcctacg
    20761 acggcaatat tttttgcttc ttccaaatag ttgaaaatcg tttcttgtgt tggatttttt
    20821 acagacattt ctgccacccc ttgcttttct ttttattcca tctattatac ttgtatcttt
    20881 cattttttca attgttcctg ttacctattc atgctaaaat aaaggatagt tttcaacttg
    20941 aaagagggta aaaaatgaaa ttgatcttat tacttatttt agcatatctt ttaggttcta
    21001 ttccttccgg tgtctggatt ggcaaattgt ttttcaaaaa agatatccga caacacggga
    21061 gcggtaatac tggaacgacc aatacatttc gtgtattagg caaaacagcc gggattgttg
    21121 tcctgcttat ggatatttta aaaggtacat tggctacttg tttgccgatg atcttccacc
    21181 tttctgggat taaccctctt tggtttgggg tatgcgccat tcttggccat acatttccga
    21241 tttttgctgg gttcaaagga ggaaaagctg ttgcgacaag tgcaggaatg cttttaggat
    21301 ttaatcctgt attttttgtt tattcttgta tcatctttat cgttagttta ttttgtacaa
    21361 gtatggtgag tttgagcagt atgatcagtg cagtattgat cactttatct acgatcgtcc
    21421 ttccttatgt agctcctgtg attttggcta agcccaattg gttactgacg atcattgcct
    21481 ttttagtttc ttcctttatc ttttatcggc ataaagacaa tatcaagcgt atcagaaacc
    21541 atactgaaag tcgaatcccg tttggtttga atagcagcaa gaaaaaatcc tgatactttt
    21601 tcattccaaa gagataaaac gggagtgtga caaatcgatg tcacactccc ttattactta
    21661 caggatagct tttttacaca ccttggtaaa cggtgttccc tgctaacttc tcggtcacaa
    21721 cctcttactt cactgtgatg gaatattttg tcttgaagat ttcatgggca cctagttgat
    21781 tgatccctac tttttccatc aagtttccac tactgttcac cgtgtcagcg attccgcacc
    21841 acggttcgat acaaacgaaa ggcgcttctt ttggataagg agaccagatc cctacataag
    21901 gcatatcttt atagcttaaa gttacgctat gggaagattt ttcacttcga atggcgtacg
    21961 catttagacc ccttgtttca aaaaccaagg catcttgctc aaaaagttca cgattcaaag
    22021 ccagattcgt atttgtctga cctaacgtct tttgatccat atcgatgaat gggcctttca
    22081 atggtaggcg taatcttgat ttcaatggag aaaatgccag atagaaatct tcaaatgaca
    22141 atccttcttc aagaggtaca ttgaaagcag gatgtccacc gatagaaaag aacatttctt
    22201 tttcaccggt atttgccact tgataatgaa ccgtgatgcc gtctcctcct aattcatagc
    22261 tgatcactaa ttcaaaatca aatggatact tttctaatgt ctccggcgta ctccttaaga
    22321 tgaaactagc catttcttgt ccatgttcaa tgacttcaaa atccatatcc cgagcaaatc
    22381 cgtgttgccc catttgatat gttttccctt gataaacata ctgatcatct tttaaacgtc
    22441 caacaaaagg gaataaaacc ggtgcatgtc tgccccaata cgctggatca ccttgccaga
    22501 tatactctaa accatgttct tttgatttca gactgatcaa ttccgcaccg tgttcagcga
    22561 tttcggcgat cagctggtca ttttgaataa taacagtcat tgccattcct ccaacttcga
    22621 cttacttttt aaaatgggaa ggctggaaca aacatttcta tctgccccag tccccttact
    22681 tctaataact gtgttttgct aactcgtctt taaaacggga attcaatact tttaaataag
    22741 ttcccttcat gcctaacgaa cgagattcaa tgattccagc tgattccaat tttcgtaaag
    22801 cattgacgat cacagaacga gtgatgccga tactatcggc aatattagaa gcagtcagac
    22861 gaccttcatc tccatccaat gcttcaaaga tagcttgtac agccttcaat tcgctataag
    22921 ataatgtgtt gattgccatt tggacagccg ttgcactccg tacatctgct tcgatgcttc
    22981 gagacttctg ataaaggatc tgcataccga caaccgtagc tccatattct gctaagacta
    23041 gatcgtcatc tgcaaacgat tgttccatac gagataagat aatcgtaccc aaacgttcgc
    23101 cagctccaaa gatcggaacg acagtcgtca gaccaaatgg atatttctct cttgactcta
    23161 ccggaaatgc agtaatatca ctagtaattg ggatattagc ttcagttttc atcaactgtg
    23221 ccactgtatc agtataagat tcaggaaatt gtttctcaac aaacattgtt ttgattcgtt
    23281 cattgttcac atcgtgacgt tcattataac ctagtaatac tccgtcactt cctatgatat
    23341 aggtgttgct gtcaagaata ccacctagtg tgacagccat tttatcgtaa ggcaaatctg
    23401 cttgcatgtc aaaagtattc ttttgctgca aaagtgtgtt tatcttgcgg gttttcgtta
    23461 gcaagtctgt cattgtcgtc ctccgatttt ttataaaata tagcgactta aatcttcatt
    23521 tgccgcaatt gagccaagtt tgtcatccac atatgcttct gtgatcgtta tttctcccat
    23581 ttgcatatct ggtgcttcaa ataatagatc ttctagtagt ttttccaaaa tggtatgcag
    23641 tctgcgtgcg ccgatattat ctgtgtcacg gttgacatcg aaagcgattt gagcgatccg
    23701 ttcaattgct tcttttgtaa acgtaactga cacattttct gtaccgatca acgcgacgta
    23761 ttgttttact aaagcattat ttggttcggt cagaatcttc acaaaatctt cagctgtcaa
    23821 atcatctaat tctacccgta tcgggaagcg tccttgaagt tctggaatta gatcacttgg
    23881 tttagacaaa tggaaagctc ctgaagcaat gaataggacg tgatctgttt ggatgatccc
    23941 gtattttgtg tttacttgtg aaccttcgac aattggcaat atgtctcttt ggacgccttc
    24001 tctggatact tctccagatt gctgtgattt agaagtgatc ttatcgattt catcgataaa
    24061 aataattcca ctttcttccg ctaatttgat tgcctcacta tggatatcag catccttgac
    24121 taattttgct gattcttcct tgatcaataa ctctaaagct tctttgactg ttaccgtccg
    24181 ttccactttt ttcttcggtg tcaaagcacc tagagtttca tttaggtcga tccccatttg
    24241 ctccaatcca ttgttcatca ttggtgcagg tttcttttgg tcttcaactt caatggttac
    24301 ttcacgacta tccagtaaac ccttttgtag ttgttccaag actgtacggc gattgattcg
    24361 aatatcttct gtcacttctt cttgcggttc agcggtattt tgctgactag cattaaaaag
    24421 attcatcatt tgttcaaatt gattagtgga ttgtttctgt tcttttttga taccaggtac
    24481 caagattttt accaaacgtt tttctgcatt ttttttagca cttgtgtata cacggctgta
    24541 ctgctctttt tctacgattt ggatcgcatt ttctactaaa tcccgaacca ttgattctac
    24601 atctcttccg acataaccga cttcagtaaa ttttgttgct tcgactttga caaatggtgc
    24661 attgacgatt tttgccaaac gtcgtgcgat ttctgtttta ccgacccctg taggtccgat
    24721 catcagcata ttttttggtg taatatcttg ttgcattttt tcatctaact gcaaacggcg
    24781 atagcggtta cgcaaagcaa cggcaacaga tttttttgct gcgtgctgtc ctacgatata
    24841 ttcatctaat tcacttacga tttgtcttgg tgtttgtttt aattcataca tatctactca
    24901 cctcttacag ttcttctacg ataatattat gattagtaaa gatacagata tctgcggcaa
    24961 tatttaacgc attttctgca atttctttcg cactcatgtc gttttgagca tagtttttca
    25021 ttgctcgggc tgcagccaac gcaaaattcc ctcctgatcc gatagctaag ataccatcat
    25081 ctggtgtgat aacttctcct gtacctgaaa caagaagcat ttctttatca ttcattacga
    25141 tcagcatcgc ttcaagtttt tgcatagatt gctgtgtccg ccattcttga gctaattcga
    25201 cagctgcgcg ttgtagattc ccgttatatt cgtttagttt gccttcaaat ttttcttcta
    25261 aagtaaacgc atctgcaaca cttccggcga aacctacaac aacttctcca ttgtagatac
    25321 gtcgtacttt ctttgcagtg cctttcatga caacagattc acccattgtt acttggccat
    25381 ctccagccat agcaaatttc ccgtcttttt cgacggcaca gatagtggtt gaatgaaatg
    25441 gtgattcgac catgtttgtt tcctcctaaa tttgttcttt tccttatttt tacgctcttg
    25501 gatggaaaga acgatagttt ttttgtaaac tttcttttgt tacatgggca tagatctggg
    25561 tagtcgataa attggcatgt cctagcaatt cttggactgt ccgcaaatct gccccattat
    25621 ttaataaatg agtagcaaat gtatggcgca gcatgtgagg atggatatca ctatccaaac
    25681 tgcttttttt gatcatttga ttcaagacat attcaattcc agtaggcgtg attggttttc
    25741 catgatgatt cacaaaaacg atatcatgct cttcgtgata ttttgtcatc aatacctggc
    25801 ggccttccct gaaatattct tgcagagcat cttgagcaaa agaacctaga ggagcatatc
    25861 tctctttgtt ccctttacca tggatcaaca tgacctctga atcaaaatca atgtctgata
    25921 ccgacaaact gctacactca cttaaacgga tgcctgaccc gtaaaggact tccagtaaag
    25981 cgcggttcct caaatccaaa ggactatctc ctgctacgct gtcaaaaagt gcttgcattt
    26041 cattttcata aaaaaaacgt ggcagtctca aatttttctt tttgagatga acgtaagaaa
    26101 atggattttc ttttatcact tcttctttga gaagatactg ataaaacgaa cgtaggctgg
    26161 ctatcttgcg actgatagaa tttctgctat agctaggacg atgatcagct aatgcactca
    26221 aatagacacg aaccgtaaaa tattccgcct tcagcagatc agcgtctcca gattcctcca
    26281 aaaaatgttt gaaatggaga atgtcttctt catatgcttc tttcgttttt tctgaatatc
    26341 ctctttcgac aatcaaataa cgcaaaaatt tttgttccca cgtggcttct gtcattttct
    26401 caccttcctt ttgtcccaga gaataattta gcacagcgtt tataaaaaga caaataaatt
    26461 gtcagaattt aaccaatatt tctaagtttg ttcacaattg gcgcaattcc tcacattttg
    26521 tttgagaaat tcttaagaaa aacgcaagaa aaagagcgag actatccgtc tcaccctctt
    26581 catggagttt ccttactcat ttgggattgc agttttttct aattctgttt ttattgcctc
    26641 attagctttt aaagctcgtt gtgcaattgc ttcatatctt gcttttttgt ctcgaatgcg
    26701 ctctggcagt tcaggaaaca gaccatagtt ggcattcatc ggttggaaat gttttccttc
    26761 cgcatgagta atgtagtaag ccatacttcc aatagctgtt tcttgtggaa actcgactgg
    26821 ttccatttct tttgctaagc gtgcggcgtt gattccagca agtagtccgc tcgctgcact
    26881 ttcaacatag ccttctacac cagtcatctg acctgcaaag aacagatctt cgcgtttcgt
    26941 tgattggtaa gtcggtttca gcaattctgg cgaattcata aagctgtttc ggtgcatcac
    27001 accatagcga acgaattcag cattttctaa cccaggaatc atccggaaaa cacgtttttg
    27061 ctcgccccat ttcaagtgag tctgaaagcc cacgatgttg tataaagaag ctgcagcatt
    27121 gtcttgacgc agctggataa cagcataagg acgtttgcca gttttaggat cttccaatcc
    27181 aactggtttc ataggtccga aaagcatcgt tttgaatcct cgctgtgcca ttacttcaat
    27241 tggcatacat ccttcaaaat atttttcttt ctcaaaagat ttaagttctg ccacttcggc
    27301 attgattagt gcctcatgga aagctttgaa ttcctcttct gtcatcggac agttcaagta
    27361 agctgcttct cctttgtcat agcgggactt caaatatact ttatccatat cgatcgtagc
    27421 tttgtctacg attggtgcag ctgcatcata gaaataaaaa ccatctgaac cattgaatgc
    27481 tttgatgctt tctgctaatg gatgagaagt caaaggtcct gttgctacta cggtgattcc
    27541 ttccggtatt tcagtaattt cttcgtttat cactgttact aatggatgct ctttgacttt
    27601 tcgggtaatt gtttcagaaa aagaatcgcg gtcaacagct aaggcaccac cagctggcac
    27661 tgctgtttca tctgccgaag tgattactac cgaattcaat cggcgcattt cttctttcaa
    27721 taaccctact gcattagcca aactgtttcc tcttaatgaa ttggaacata ctagttcagc
    27781 aaaattactt gtgtgatgcg cctcagtcga tttgactgga cgcatttcat ataaacggac
    27841 agggactcct gcttgtgcga tctgccaagc agcttcactt ccggcaagcc ctgctccaac
    27901 aacatttacc attttcaatg aaagaacctc ttttctatta tttttggatg tcttcttcgt
    27961 aatctccatt tacacagact acttgtttac cgcctttgac tttcttctcg accagatatc
    28021 cgtcacattt tggacaattt ctgcccactg gtttatccca agaagtgaat tcacattctg
    28081 ggaaacggga acaaccatag aatagtctgt ttttcttaga ttttcgttca atgacttgtc
    28141 cttcatgaca tactgggcaa gtcacaccga tttcttttac gattggtttc gtattccgac
    28201 aatcaggaaa attactgcaa gcataaaact tcccatattt ccctaatttt atcaccatcg
    28261 gatgtccaca gacatcacaa tcgaatcctg ctggttcatc tttgatttga attttttcga
    28321 ttttttcttc cgcattcgtc agttcttttt caaacggttt atagaaacga tcaaccactt
    28381 tgacccacgc ttccgtgcct tcttcaattt tatccaaatc tccttccatt tctgctgtga
    28441 aatggatatc gacgatttga gggaagaatt cacagattaa tgaattgaca atttctccta
    28501 gttcagtcgg ctcaaatctt ttttgtgtca attttacata gtagcgtctt tggatcgtat
    28561 caagtgttgg tgcataagtg gaaggtcgcc ctaccccgtt ttcttctagt gcacgaatca
    28621 acgtagcctc actaaaacgt gctggtggtt gagtaaaatg ttgttttggt tcaatatcaa
    28681 cagattggac tacatcgcct tcttcaagtt ctggaagaat attttctttc tcttctttgc
    28741 cgtcatctct accttccaca tacacttgca tgaatccttt gaatttgatt tttgaaccgt
    28801 tcgcaatgaa gatgacacca ttttgttcta atgtcacttt cattgtatct aaaatagctg
    28861 gtgtcatctg actagccaca aaacgtgacc agatcaatgt ataaagtttc aattgatctt
    28921 tatctaaata ttgtttgagt tcatctggtg tacgaagcac actcgttgga cgcacagctt
    28981 catgggcatc ttgtgcgcct tgagcatttt tgatctttcg gtgatcatgt ctagaaaatt
    29041 cttttccata cgtatcttca ataaatgctg cggcctctgc ttttgctgta tcggcaatcc
    29101 gggtagaatc agtacgcatg tatgtaatca agccgactgt tccttgttta cccaaagtga
    29161 ttccttcata taattgctgt gctaccatca tggtttttct tgttcgaaag tttaacttac
    29221 gggcagcttc ttgttgcagg ctacttgtag tgaaaggatt cgcagggttt cttttgcgct
    29281 ctttcttttc tactttttta acttcgaagt ctgttccatc aattctagca gtgacttctt
    29341 ttacactttg tgcatctggt aatttctttt tcttaccgtc gattccccag aaatttgctt
    29401 tgaatttctt gcgagctttt ttaaaattac cgtcgatgct ccaatattct tctggaacaa
    29461 attctcggat ctctttttca cggtcaataa taatttttaa agcgacagat tggacacgtc
    29521 ctgcacttaa ccctttcttg accttacgcc agagaatagg actgatcgaa taaccaacga
    29581 ttctgtctaa ggtacgacga gcttgctgtg catctactaa atctacatcg atcgttcttg
    29641 gttccttaaa agctgctttg actgcgtctt ttgtgatttc attgaagacc acccgatttt
    29701 tttctttcag atccaaccca agtaggtacg ctaaatgcca agcaatcgct tctccttctc
    29761 tatccgggtc acttgccaag taaacttttt gagctttttt tgctgcggct ttcaggcttt
    29821 tgatgacatc gccttttccg cgtatagaaa tataatgggg ttcataattg ttttcaaaat
    29881 cgatacccat tttacttttc ggtaaatctc ttatatggcc aacactggcc attactttat
    29941 agtttcgtcc aagatatttc tcaattgtct tggcttttgc aggtgattct acaatcacta
    30001 aatatttata cgccattact gaatggactc ctttccattg tcgtgttcct actattaaaa
    30061 gaggattcat acaaatcgta gctaatcata tccattcaga aaacgtttgt caagaaaagc
    30121 aaatgaaaag tatggtgtta ttctttaaat tctgccaaga tatcttcacc cgaaaccgtg
    30181 caaactgcac cgtcttggat caaatgatga catccggtag aatgttctgt taaaatattt
    30241 cctggaacag aaaaaactgt tcttccaaga tccaatgctt gctgtgctgt tatcagagag
    30301 ccgctttttt tcttggcttc aaagacacat gttcctttag acaaagctgc gataatccga
    30361 tttctcattg gaaaacaatg ctttttcggg ccggattgag gaggatattc actcaaaaga
    30421 agatgatcat ttgcaatcac ctcttgcaag tcacgatttt cagcaggata gacttgctct
    30481 atccctgttc caatgactgc gattgtccgt ccaccatgag agattgctgc ttggtgaccg
    30541 tatgtatcta tccctttcgc gcagccactg acgatcacat atccagcttc tatcactggt
    30601 tttagtaaat aataaactgt ttttttggct aaagcagaag tgtgtcttcc accaacaacg
    30661 cttaacatgt tacaagaaag taaagataga tcaccattat aaaacagtac aattggcgga
    30721 taggtcaagt gaagcagctg agggggatat ttgggatcaa agcaagtgat aaacgactgt
    30781 ttcgattgga ttttgcccca gttagcgtga atttcctgcc aacttttgcg aaacagctcc
    30841 cggtgtttgg ttattttacc tatcttaatg atgtcttcaa atccgatttc tttataagga
    30901 tactgcatca tccagttagc aagtgcccat tttccttgac tactgattcc ttttgtataa
    30961 gcaagtttca ataaattttc ttctatttga tacatcataa aaccgccttt cgtctctctt
    31021 tctaataaga tacgtaaaag cggctgtttt ttattgtaaa tattttttta caggtgcaaa
    31081 acttttacga tggattggcg taattccttg tcgttcaagt ccttctaagt gttcctttgt
    31141 tccataacct gcattatgcg agaaaccata tccaggataa agcacgtcat attctttcat
    31201 caattggtct cgatatactt tagcaatgat acttgcagca gcaatcgaca cagatcgtgc
    31261 atcccctttg attatttttt cctgctgaat aggcaaatcc aaggtcatcg catcgatcaa
    31321 caaatagctt ggtacaatgg acaatttttc tactgcttgg atcatcgcta atttacttgc
    31381 ttcataaata ttcacatcat cgattgtctg ctgatcaacg atcccgatcc cgattgccag
    31441 ggctttttct tgaacttgtt gataaagaag ttctcgcttt ttctctgata attgtttgga
    31501 gtcattcagc cctaatatga tctcattttt cggcaagata actgcagctg ccacgacagg
    31561 tccagctaat ggtcctcttc ctacttcatc aatgccggca atccattggt gaccttgtgc
    31621 gtacccttgc ttttcaaagc aaaacatttc ttcatatttt tccttaatta atgcttcctt
    31681 tttcaatcgt cgttcaaaag aagccagtgc ttgctgcacg cctttgcgtg gatcatttct
    31741 taatgattct agtgtaggat catctattct ctgtatgctt ttcaatcgtt ctttgatact
    31801 ttggatggat tcagtcatca cttttttgct ccatttcttc gcaacgatcc agtgtgtatc
    31861 gtccaagttt tccttgacga atatcatgca cgactaattc gctgccacga tcatagtctt
    31921 ctttataccc acgttttgct gtaatatcca tcagtaattc cggtgccaat ttttcttctt
    31981 gctcgattga taaaccataa cgatcgctga ttcttcctga ataatatcgg gcaaaaaagt
    32041 ctaatccgta aatcgctaaa tcatctaaat ggacaagctg gtctttgatc gcgcctgtca
    32101 gcgctagttt tttgccgatt tcttcatctt caaatttggg ccataaaatc cctggtgtgt
    32161 ctaacaattc caactcatta cctaaacgaa gccactgctg accttttgtt acgccaggct
    32221 tatttcctgt ctgtgcaatt ttcttgccta ctagtcgatt catcaacgta gatttcccaa
    32281 catttggaat acctaagacc attgcccgaa tagcgcgagg cttgatcccc cgagcagcat
    32341 ctcttgcccg tttttctttc aatgcttctt tagccgcagg tactagacct ttaattcctt
    32401 tactttcttg tgccgtgata gctaatgcaa gatggccttg tttacgaaaa tattcttgcc
    32461 acagctttgt ctgttgtgga tctgccagat ccgctttatt caataaaaca aggcgaggtt
    32521 tttgctgaat gatctgatct agcattggat tacgagaaga taaaggtaat cgtgcgtcta
    32581 ccaattcaaa tacgatgtcc acatatttca ttttttcaga aacttctcgt cgggcttttg
    32641 ccatatgtcc cggaaaccat tggatggtca ttgttctctc cattcctttc ataccggaat
    32701 attccccggc tttacttgtt tttttctttt tcaatataaa ttcacttttt tattataaag
    32761 aaattccatc tattagttaa gtgaaagtga accagtcctc attctatcac atcttcactt
    32821 ttttgatgat gcaaattttt attttggttt ctatatatga atctaaattt ttgacagcgt
    32881 ttcctaaata agagatgata gacgaacaaa acagaaagga gacgaaacaa atgaatagta
    32941 tggtttttgt taattttcct gttgctgatg tcaatcgttc cgttgctttt tatgaaaagc
    33001 tgggttttaa aaaaaatgaa gaattttcta ctgaagaagc tggttcaatg gtatgggatg
    33061 acactttttg gataatgctt ctctctcatg atttttataa aaaatttata aaagataaag
    33121 aaatagctga taataaaaaa acaagtggtg ctttgatttc gtttagtgtt gaaaatgcgg
    33181 atgaagtaaa aaaatttgct caaattgcaa aagaaaatgg tggtactttc cacgaagtag
    33241 atatgggaat gcctgaagaa gaaatgtttt cattagaagt ccaagatcta gacggaaata
    33301 cattagaacc cgtatggatg aagatgtaag cagataacta atctgacgtt ttttataaca
    33361 aaaaaacaaa ggcggatgat acgataagta ttctccgcct ctattttttt tattcgtaca
    33421 atctcttttt tacagtttat aaaagcagtc ttctcaccct ttttttaatt gttagatgcc
    33481 agtgtatgta aatcaccaat gtgtcatgac acattggtga ttttatggta tccttttatt
    33541 attaactgac aaaggagaga atattttgaa cactttgatt gcaaaagacg tttttagatt
    33601 gcccgaaatg aatgcaagat tgttgtcgac ctctgctctt ttattagccc ttgatgtcat
    33661 cttatacaag atagcgattg gtcctgctta ttttcaaatc acattcggat ttttaagtat
    33721 ggcgcttatg ggttatctat atggaccgat ctgggctggt ttgcttgaaa tgttgtcgaa
    33781 cactttaaat ttcacgatat ttggttcagg aacgtttcaa gctgcttttc ttatcacggc
    33841 tttccttggt ggatttatta atggcttatt tttatatcat aaaaagataa acctgccttc
    33901 cgtcatcgct gcccaattat tgattatgat cccagtcagc ctttttatca attcttggct
    33961 gatgtctgtt ttgtttggtg cagatttcaa agtaattttt ctaggtagat tcatcaggaa
    34021 tatgatccaa atccctattc aaataatggt tgtttattca tttatcaaaa ctttagaggc
    34081 tagaaaagtg tttaaacgag ctgtttgatt ttacgtacta aggtagtaaa ctgattttgg
    34141 attaaacaat aaagcgtgca gtctcaacag actgcacgct ttattgtttc tcactggatc
    34201 cattttattt catcaagtgg ataatagaca aatcttgctt tacctagaat atcagtttta
    34261 ttgatcgtgc caaacgaacg actatctttt gaaactcttc gattatctcc aagtacaaaa
    34321 taattatctt ccggaagctt ggattgtccc gttagctctt gtagtgtaaa gtccgtcgta
    34381 taggatgcat ttgcatgatc tgatttcagg tttttagtta gataaggttc cttaactact
    34441 ttcccattga tcttcaattg atcattttgg tagctgacat tttctcctgg aagaccgatt
    34501 actcgtttga tataaatcgt gccatctgct aattgaaaaa caacaatatc aaatcgtctg
    34561 atctccgaaa atttttccat cacaaccata tcaccttgct tcaacacgtt ttccattgaa
    34621 ttgccttcca ctggtaccgg gatgaataaa aatccccgca acatgaacgc aaggaataca
    34681 gaaacaagta agtatttgaa tacaagccag aagcgattaa tatagcgttg tttttttgtc
    34741 aatctctatc aatccatccc tctttttttc attctttctt tattataaga aaaatatcaa
    34801 aaaaatgcga ctagatagct gaaataaaaa taattctttc aattaggcta ttggacaaaa
    34861 aacttttttg atcataaagt acaaaactat ctcttatatg tcatgattat tttctaaata
    34921 tttttcccat gcttcgtcaa agatatccat ggacggcaaa tagtcgacat tcagttccag
    34981 ataattagaa atttcttcgt agctagagct gtgcttagga aattggatgt ctttaccggc
    35041 ttctgtagcg aaaacagata aagcatcatg tgtgccaccg cctcgcagtg tcattaaata
    35101 atgaaaaaag cttcgattca cccttcgttc ctccaatttt cttcgataaa ggcagcccgg
    35161 cgtgcatggt ttcttttaaa attttcagga tgctttttat aatactcttg atggtcttct
    35221 tctgctagat aaaatggttg ggctggttcg atttttgtaa cgattggctg ttcgaaacgt
    35281 ccactttcag ctaactgttt cttacttttt tcagcaatga tccgctgttc ttctgtcaaa
    35341 taaaagatca ccgggcgata attgtcaccg cgatcctcga attgtccgaa agcgtcagtt
    35401 ggatcagttt gctgccagta aagttctact aattgttcat atgaaatcaa tgttggatca
    35461 tagataatct ccactgcttc tgtatgtcca gtcgtttggc tttttacttg ttcataagtc
    35521 ggatctggta catgccctcc agtatatcct gacatcactg tatggatacc aggaagcgtg
    35581 tcgaaaggct ggatcataca ccagaagcag cctcctgcaa atattgctcg ttccattatg
    35641 attccccctc taatactact catccgatga ttttttattt tcttttgtct tgctttcagt
    35701 actgctggtg ctagttttgt tggtatttga gttgtttgaa gcatcagagg tatcttttgg
    35761 caaatataga ctaaaccgaa tgtcatcatt tactaagtcg attttatctg ctttaatata
    35821 catcccgttt tgcattcgga attgatccaa cctaagcatg atagtcagat catctgtatt
    35881 gacttcgatc caatctggta atttatagtt tctcttgatc atattcatca catctttgat
    35941 tggcaaactc aatgttccga tagaaagact tctagctttt agttggacat ttccgttttc
    36001 catcacatac ggatcaaaat ataaatagaa atcgatagga aaacctagta cttcgaaagt
    36061 accattgagt agtgcttcat tttccaaata aaatttatat tcaatatcac tatctttttg
    36121 atactcacta aggaaaaagc tgatgatttg attgactttt tctttatttg aattgatcgt
    36181 taagacagga tctccttgtc tttccgtaag agctggtatt tctgggagtt ctggttcccg
    36241 attagcaaaa atacggctgc ctaaaaatac ggtcgaacca agtactaccc caagaaggat
    36301 aaggaaggcg aatttccagt aatttgtttt ctttgttttg tttgaagata cgtcagatct
    36361 tgttttcatg tttattgatc atcctccttc tctaaccatt ggttttgtgt ctcgatcagc
    36421 tgatctctca ctgcatttgc catcaattga taacctaaat tatttggatg gaaatgatct
    36481 tcttcataga gcacattatt tttgatgcca tttagatttt ctgtcgtgct agttgtttga
    36541 tctgttcctt cttctgtgac acctacttcc tgattcaatc cttgataaag caaatcattg
    36601 attgggataa agtagcagtt cggcattttt tttgtcaccg tttctgttcc tttgttccaa
    36661 ttgttaacaa ttgtctgcat gtcagtgatc tcaggaaagt tcaaataaaa tggattgtag
    36721 atgcctaaaa tgtaaatagg tgcggtttta ttgagggatc gaaattcttc caatagttct
    36781 gtgacatttt cctgatagtt tttcagtggc tttttaaagg aattgaccgt taacccgaat
    36841 aaattatttt ggataacttt catcaaatca ttaccgccaa cagtcagtgt gataaaatca
    36901 gctgttttga tattgtttcg aatttcttct tttgttttta ctcgctttaa gatctggtca
    36961 cttcgttcac catttacacc atagttttct gtttcaaccg cggtcaaatt ataacggtct
    37021 tttagatcat ttgcgacaag gggaacgaat cctccttgct tggtttgatc tcctactcct
    37081 tcagtcagcg aatctccgat agctatgtag tgaagttttt cttttttatt tggatcttct
    37141 ctcacagtag ttactggttt cattagtggg ccagctttcg gtatagaagc agctaaacct
    37201 gcatagatga tgatcgcaat taataatggg aacaaaacga ggatcaaatg tcgtttcgtt
    37261 aatttcattt tttcacctct tgtattcgat tggttctaat gttatcaaaa attttatcaa
    37321 aaaggtagtc tttatgaaaa aagaaaacaa ttattttcct aattttataa agaaaaagag
    37381 aataagctgc aagcctttca ggctgagggc ttatcctctt ttcgactaat ctacataata
    37441 catgattgca aacgcgtctt taccagtatg cgtagcgata accggattcg tatgcagtac
    37501 agggatatgc atgtccggga ataattcttg caaaccctct ttgaattctt gcgccaattc
    37561 ttttgcatcc gcatgtgaaa taccgatttg tcgcacattt ggcaattctt tcagttcatt
    37621 tttcaaatta ttaaaccatt tatcgaacgt tttctttcct cgccccttag ttacagggat
    37681 taattcagaa tgggcgaaat tcatcaccac gcgtatgttc aaaacactag acagcaaacc
    37741 agttgttcgg ctgattcttc caccttttac caagttatct agtgtggata ttccgatgta
    37801 taattttgta ttgtcttgta cttttttgac ttcttcaagg attttctcta ctgttttacc
    37861 ttcttttgct aattcagctg ctcgaattac ctgaaaagat aatccttggt cagtggtatc
    37921 actgtcgata accgttactt tcgtttttgt tagattactg gcttgtcttg ctgcttctac
    37981 tgttccactc aagccttttg tcatatggat cgaaatcact tcactgccgt cttctcctag
    38041 ctcatcatac atcgagatga attctccaat tggtggttgg cttgttttgg gcagattttt
    38101 tgctttcgcc atcatttcca taaatctttc gccgtctagt tggtcatcat cgggatatac
    38161 tgttccgtcg atcatgatgg atagcggcat gacacgaatg cctaaagcat cgcgtacatt
    38221 tttttccatc gtacatgaag aatctgttac gatttttaca tttgtcatta ttttctctct
    38281 ctctttcttt aacaagatgc cttattcatg ttaaactaca tgtaagacaa tttctatttt
    38341 tagtataaca gacaaataat gatttttcat taattacacc tagtaaagga gccgttttca
    38401 tggaaaaaac gaaatttaat cgaacgtatg aaatattaaa tgaagtgttt aatgcagtca
    38461 cccatggaat tgggactgga ttgagtatcg cgggactagt ggttctttta gtaaaaggtg
    38521 cccacttgca ttcctccctc catattgtct cttatgctat ctatggatcg atgatgattc
    38581 ttttattttt aacgtctact ttgtttcatt cattgatttt tacaaaagca aaaaaggtgt
    38641 tccaagtatt cgatcatagt tcgatttttc ttttgattgc aggaagttat actccttttt
    38701 gtttgctgag tatccgagga tggctaggtt ggactttatt tgtattaatc tggttgtttg
    38761 cgattcttgg cattatatat aaagcgttga cgttgcataa acgggagaga gtatcaaaag
    38821 tatctacgat catctatatt tttatggggt ggctctgtgt cgtggctgct gttcctcttt
    38881 atcactctct tggaatgact ggtgttattt tgatggtagc aggcgggctt tcttacacga
    38941 taggcgcatt cttttatagc ttgaattcta tccgctatat gcatgttgtt tggcatttgt
    39001 ttgttatgtt aggtgcaggt tttatgtact tctctgtttt attctttact taataaaaaa
    39061 aataaagcta tggatgggac agctctaaat aacagctagt ctatccatag cttttttata
    39121 tcgtcaattc gcctgtcccg ctttttacag gattctcatt acgctgatac gtttcaaatt
    39181 gatgcggata acgatttttt tcatccattt ctccttgact agtattgatc agtgaccaat
    39241 cagaccagtc aacaggtgga aaataagcat ctccatcgaa tacatgatgg atgactgtac
    39301 ggtataatat cgtacaaaat ggcagaaatt cttgataaac agctgaccct ccagcgatga
    39361 aaaagatacc gtctgtttct ttttccagag caagcacgtc ttccacacta tgcatgacat
    39421 gcacgccttc tgctttatag gaagaatctc tcgtcaaaac aatcgtctga cgattcggca
    39481 acggacggct acccatccct tcgaatgttt ttcgtcccat cactagtgta tttgcttctg
    39541 tcatttgttt gaagaatttc agatcatttg gcaaacgcca aggaagacgg tcttcttttc
    39601 caatcaatcc gttttcatcc tgtgcccaaa ttgcagctaa cattttctca tcctttctga
    39661 ttctaaacag cgataggagc tttgatcgcc ggatgtgggt catatccttc taattggata
    39721 tcttccatct caaaatcaaa tactgactgc ttttcctgat tcaaaactaa agtcggaaat
    39781 gcgcgtattt ctcggctcaa ttgttctttc atttgttcga tatgattgat ataaagatga
    39841 gcatctccta atgtgtgaac aaattcgcca accgctaaac ctgtttcatg tgcaattagg
    39901 tgagtcagca acgcataact tgcaatgttg aacggaacgc ctagaaacac atccccactg
    39961 cgttgataca attggcagct tagccgtccc tcttgaacat aaaattgaaa catcgtatga
    40021 caaggaggca aagccatgct aggaacatct tccggattcc atgcagaaac gatcaatctt
    40081 ctagaatcag gagtcttttt gattgcttcg atcacttgtt tgatttgatc gataaagcca
    40141 ccgtcttttg tttcccagtg acgccattga tagccgtaga tgtgtccaag atcgccgtat
    40201 gtttcagcaa aggaatcatc ggttaggatc ttctcgcaaa aaagcttgct ctgcttttta
    40261 tattcttcat tgaattcagg atctgtcaaa catctatggc caaaatcagt catatctggt
    40321 ccattatagt ctggactttt gatgtagcgt tcaaatgccc attcatccca aatatggttt
    40381 ttattttgta atagatagcg gatattcgta tctcctttta aaaaccaaag caattcgctt
    40441 ttgatcaatc caaaaggtac tcgtttcgtt gttaacaaag gaaacccttt ttgcagatca
    40501 aaccgcattt ggtatccgaa taagcttaat gtgcctgtgc ctgttctgtc ttctttacga
    40561 tgcccttctt ccaacaattt tcttcctaag tcaagatacg cttgttccat cctttttcct
    40621 cctaatcttc tacccattca ctcaagtatt cccaccgtgc cattttttct tcaagttgtg
    40681 cttctgtttc agatacgtcg ttttgcaatt cttgtaatcg agtgaaatta tctccttgat
    40741 gattcatctc ttcttggagc aatgagattt tttcttcaag ttctgcaatc tcatcttcaa
    40801 tcgtttccca ttctttttgt tccatgtaag ataatttctt cttttcagca ggttccttgc
    40861 tctgtatttt tggtttcgct ttttcagcag gctgctgggt ctctttttgc ttcagtaaat
    40921 aactggacat gtttccgtaa taaagttctt gttttccgtt tccttggaat actaataact
    40981 tatctgctac cttgtccaag aaataacggt catgggaaac agtgatcaca gcacctggaa
    41041 aacttctaaa ataatcttcc aaaatcgtca atgttgctat gtccaaatca ttggtcggtt
    41101 catccaataa taaaacatta ggttgctgaa tcagcagttt caacaaaaat aaccggcgtt
    41161 tttctccgcc tgaaagtttg cctatcagtg tcccatgcat aaatcttgga aataaaaatc
    41221 gttcaagtaa ttctgctact ccgatttgac tgccatctgc tgtttttgct tcttcagcag
    41281 cttcttgtaa gtaagcaatc atccgtttat ctggtgccat ttcttcattt tcttgtgtat
    41341 aatacgcaag atgaaccgtt tcaccgatag acattgtacc gctatccagg ggaattctgc
    41401 cagctagaat attaagcaat gtcgattttc ccgcaccatt tttaccagta atacctagtc
    41461 tttcccgaga ttgaatcaat aaatctaaat gttctagcag tgtttggtca tttatacggt
    41521 aactgccatc tttgatttcc agtacttttt tccctaaacg ttgtgtcgct aggttcagct
    41581 ctaaatcatc ttcttggttt acttggtaca agttctcctt tagatcttcg aaacggttga
    41641 tccgtgcttg ctgcttggtg ccacgtgcct gtacgccagc cctcatccaa gctaattcct
    41701 gtttataaag ccgcttgcgc ttttcttctt gttctacttc tacacgatca cgttctgctt
    41761 tttctaagac gtaagcttca taatttcctt tatactcgta taattttcca aatgaaagtt
    41821 cgaagattcg gtttgctact ctatctagaa aataacgatc gtgggtaacc atcaatagtg
    41881 cgccacggta ttgtttcaaa tagttttcca accactcgat cgcttcataa tctaaatggt
    41941 ttgttggttc gtctagtaat agtaaatcgg gttcatcaat caacacttgc gccagactaa
    42001 ctcgtttttt ctgaccacct gataaagtac tgattttttt atctaattca ctgattccta
    42061 atttttgcaa aataatcttt gcgttggtat ctgtagtcca agcatcttct ttattcattt
    42121 gttcttctgc tttagcataa cgtttttgca catcagggtc ttctccgttt tcccctagtt
    42181 cgatcaatgc ttcctcatat gctcgaacag tttggatcaa ggggctgttt ccttgaaaga
    42241 ctgcttggag aacagttaat tcatcggaaa attcagctgc ttgagaaaga taaccaatac
    42301 ggtaatcagt tggataaaac actgtttgcc gatcaccgtc gctgctatcg ataccggcaa
    42361 taatatttaa gagacttgtt ttacccgtcc cgttcgtccc aatcaacccg atacggtctt
    42421 tttcgtggac caaaaaagaa atctggtcaa acaaatcttt ttcaccataa gtttttttga
    42481 ggtcagttac ttttaattct ttcaacatgt tccctcctta tcttggatat tgtatcataa
    42541 aaatagtgaa cttcaggaaa atttttttgg tatttgttct ttttgtgttc tcaaaaaaag
    42601 aacaggaccg attcgatcct gttctgatat gttttgtttt ttataaaact tgaaaatttt
    42661 taccttttta ttttattgga ctcgtttttt ttgactttgc tcgaaacgca aatcggaaag
    42721 aaaagttatc agatggtttg ttcatcgtat aagattctaa caaattgata ttcctgcgat
    42781 agctgttcgc agaaaacgtc gtgatttttt cagcaacttc gtattcatca atgatttttc
    42841 tttcaagata ataacctctt agcatttttt tcgtaaacag actatcttgc tggatcatcc
    42901 acgaagaaat caaattcccc gagttcattt ttattccttc aagtttgcgt tgctggataa
    42961 aaaattgaac catcgtttca tcataaatat cagtcaagtt atctaaactt tcaataatca
    43021 actgagtatt tttttgatat gtctctctga tttcttggat atcttcatca ctaataaatg
    43081 tctgacgatc agcatatctt ctttgccaaa acattttcgg atggagaacg attcgcatta
    43141 acctacggac aacaatgaac caaaagccga taaaagacaa aatctggctt gtgatagaat
    43201 gttcgaatcg agaaagaaaa cgggagtaaa aacgataacc attagaagaa agcttgcccg
    43261 aacgataact ttcatccaat ccatcccgtt cgatcgacaa aatcagtgct tgtatttctt
    43321 gtacttcttg cttttcatca tcggttaaat cttccaagta cagatcacgt atccgttctt
    43381 gataattttc tatcactgct tcagtggcta aaaattcttt tgtattacga tcaatttcct
    43441 ttatttcttt tcgcaaaact tcaacgacct cttctaaaat catcaaagca ttcaaatccg
    43501 tgcttttcgc ttcctctgtt tccgtcagca taggcaagac aatgatccct atgaccaaag
    43561 tcactaagat gacacccgcc gtcagaaaca aaagcaaaga ccgctggtaa aacatcatat
    43621 tattgattga aaatggcaaa ataaaaatcg tagccaaact gactgttccc tttacaccgc
    43681 caaatgttaa gatcaagatc tcgttcaact gttttttgaa ctttttactg ccatctttga
    43741 atacataaaa aagactgata gaaatgaatc tgatcacaaa aagcatgaca gagattaata
    43801 cgataatcgc taataaaaga ccgtttggat ataaaccgtc tccccatact ggcgagaaca
    43861 cttgcgtcaa ttcgattcct aaaaatataa atactaatgc attcagtgta aaagcaatag
    43921 ttgtccatgt actgtgggac aagctcgaca attctgcatc aaacacagag attttcctaa
    43981 aaccagaagc ttggagaata cctgctgcca ctgcagcaat gattccagat acatcaaatg
    44041 cctctgccaa tacataggct agaaaaggaa gcaaaagttc gatcagcaga taccctgtga
    44101 catcttgtgc tgatgctttt tcgatcaaat gaattatttt ttgtttgaac cagacaagca
    44161 aaaagccgat caaagctccc ccaatactag aaactactag actcatcccc gcgtttactg
    44221 ctgaaaaaga tccggtgatc agcgcaccaa gagcaaactg gaaggcagta acaccagatg
    44281 catcattcaa aagaccttct ccttccaaaa tatgcatcgc ctttttaggg atattcactc
    44341 gtccagaaag agaactgact gctaccgcat ctgtcggacc taacgctgct ccaaaagcaa
    44401 agcaggcagc aagtgggaca cttggcaaca ggaatgataa cgtcgcacct accccgacaa
    44461 gggtcaaaat gactcctcca aatgccaaga acaaaattgt cccgaaattt ttgagaatcg
    44521 aagaaatatc tgctttttct ccttctctaa ataacaatgg tgcaataatc ataactaaga
    44581 acaattcagg ttcaaaatcg attgattgtc cccactctgt cagacctaat accactccaa
    44641 ggaaaatttg gatcagtgga gctggaatca ttggcaaaat gcggctagct atgttcgata
    44701 aggtgatcgt tactgcaaat acaataacta actcagcaaa ttccatcgtc catccccttt
    44761 attttttatt ttgtgtaagc atttttaaaa attgttcttt tttctttaaa attttggttt
    44821 cgactacttc aacctgtgtt tggttcccct ctaattcaca ctgtacacgt tttgactcca
    44881 aacgaagcag atcatgtttc agacgttcca actgacgata catcaatata tttcttttac
    44941 gcaacttaca aagtatttga cggcggtcat ccatcgaaag ggcgccatag cgatcatgca
    45001 acagtttttt ttgttgttca aaactctctc tcattttcca gatctcctat acacaaactt
    45061 ccatcattat agcaaagttc tttttggatg aaaaatattt cattgtttca tcgtttattg
    45121 ctagttagaa atttccataa atttttcaac gcctcattct attccaaata gtatcagata
    45181 tcaagtaaaa cttaagaaag ataaaagttt ctgacaaaag gatgtttact ttcccattcc
    45241 tgcctttctt gttcttttat cacaacaaaa aaatggggct gcgacaaaaa tcttgtcaca
    45301 gtcccttatt ctgaataaac ggtgtggaag agacagccta tcagtagtaa taagacacat
    45361 ctctttcaca gcctcatttt tgcgtaaaaa tcgatttatt cgaaaaattc cccgtataca
    45421 tcttctacac gttgatctgg attgatcacg atatacaagt tacctgtttt ttcgctgatt
    45481 tttgttgatt gatattgatt gtctaatcct tctgttcctt tatctagata tgggaagtac
    45541 acaagatcag gtgtgccatt tcggtaaagg atttccattc gctcgatatc ttcgtatttc
    45601 aatgcgcgat taaacatgcc catttccagt ccgcctaggt tgatatcatg tgtcgcaact
    45661 gcgtcccctt catggaagat ttcgatttta aatccagcac acggatggat ttcaacaaat
    45721 tcactgccat tgattcgtcc aaaacttgtt gtgatttgtt tgatccaaag atcgccgata
    45781 tgacggcggt tgatcgtcca ggtttctcca tttctaaaat aaaattttag cgctgtcatt
    45841 tctttactca ttaaaaagac tcctttatga tgtaatttat tcttgtacta cctcagttta
    45901 gcacaaattt atgtaagtgg tcacatctta tgttttttct tctaatgacg tatctgccca
    45961 ctttaagtag ccaacaaagc tgagcagata cagtctagtg ttttttaaat caatttttct
    46021 ttcgataaaa aatccaaaac ctgtttttta tcatttttta ttctttgttc tacgacggct
    46081 ttttcagcta attgcaaagc ttcactaacc cacttaccag gtttttttcc agtttcttct
    46141 agcaagattt taccatctac tgctaattcg cttaatgact tgataggtaa acgatcatag
    46201 gattctccta cggcatccat ctgaggtgtt tcgtcaaaat agatcatcaa ttcttctact
    46261 tgcagtgcat gttttttgcc taagaagtac aattcgaatg gttccaaagc tccagtttct
    46321 cgttgtcgta gtccttggac aagtgcttga acgtctttaa tcatttggtt agaacacttc
    46381 cacgatttca aaaaggaacg aatctcattt tcttttagat ccagttggct gatcaataat
    46441 gcccatgctt ggctctcttc tttcaatggg acggatggta attcagcaaa acgtaaaagt
    46501 gcttcgcctt cttctttcaa accgggacag caaatgtagc actctgtttc aataaatgca
    46561 tggattcccg cggcccgttc atttcccaag agcatcttga tgaattccac atttatacgt
    46621 tctacagata ttttacctaa tagctcgtgg ttttctttga tggcgtagaa ggtttcttcc
    46681 tctaatttga accctagttg actgacaaaa cgcaacccgc gcatcattcg taaggcatct
    46741 tcatggaacc gctcatgagg attgcctaca gctcgcaaga tctgttcttc tagatcttgc
    46801 agaccatcga acaaatcgat gatttctcca tcttctttca aagcaaacgc attgatcgta
    46861 aaatctcttc ttttcaaatc ttctgccaac gaacgaacga attcgacatg atctggtcta
    46921 cggtagtcct gataagttga ttccgtccga aatgtggtga tctcatattg ttcatcattt
    46981 tctaatacca acacagttcc atgttcgatc ccaatatcta tcgtttttgg aaaaagctgt
    47041 ttgatttctt ctggaaatgc gcttgttgcg atatccacat catggattgg ctggttcagc
    47101 aagatatcac gaacacttcc acctacaaaa taagcttcat atcctgcatt ctctaatttt
    47161 ttcagaactg gaattgcctt ttggtattcc agaggtaatt gttttaattt catttttcca
    47221 acctttcctg cgtgtttctt gtcctgagat aaagactcag ttgcatggtg actgtttgac
    47281 aagtccgttt cagagtcttc aaggccagga ccacttcatc ttgtttactc gtccttacgc
    47341 tcaaagcgat tatggtctct ttgattgaat ttttccatgt ttttttcaaa aacttcagtt
    47401 aagtcgatat tcaatgaatt agccatgatc atcgtaacga ataaaacatc accaagttct
    47461 tctgctaccg agttaactgg ttcagaagcc tttttgcttt tttctccata agtgtggttg
    47521 acttctcttg ccaattcacc tacttcttca gttaatcgag ccatctgagc taatggagag
    47581 aaatacccca ccttgaattg ctggatatat tcatccactt cctgctgcat actgtataaa
    47641 gaacgctttt tatccatctt tttccctcca aaactatctt accaaaactt taacgcaaat
    47701 cctagataag agcaattgag aaaatcgaat ttccatgcta catttaggaa ggaatttcta
    47761 aatggaaatt ctgtgaaaat acataattga aagctggaat tcatatggtc aaacgaaaaa
    47821 cagtaaaaga agttcttttt atcattatag gaacaagtat ttatgcattt ggtcttgttt
    47881 acttgaacat cgctaatcat ttagcagaag ggggagtctc agggatcacc ttgatcttaa
    47941 gagctttatt tggtattgat ccagcttata cgactttatt aatcaacatc ccattgattt
    48001 tactaggagg aaagatcctc ggtaaacgtt ccttggccta tactgttcta ggtacacttt
    48061 ctttgtcatt ctttttatgg atctggcaaa gggttccact agaaatcaat ttacagcatg
    48121 atctactgat cgtttctcta cttgcgggtt tagttgcagg tgtaggaagc gggatcgtct
    48181 accgcatggg tggaacaaca ggcggcagtg atattatcgc tcggatttta gaacaaaatt
    48241 acggcatcac tatggggcgt tccctattgg catttgatat catcgtctta cttgcttcat
    48301 taacctatat cgacttgaaa cgtatgatgt atacgttgat tgtttcgtat gttttcagtc
    48361 gtgtgattga ttcgatctta gatggcggat attctgcaaa aggtctactt gtcgtctcca
    48421 acaaaagcga agagattgca ccactactga tgaccggtct gcaaagaggt gtgactttcc
    48481 tttctggaga aggcgcattc tctggtaatt caaaaaaaat catctatatg gtcgttagct
    48541 ctagtgaatt aactgaagct aagaggatcg tccacgagat tgacgaaaaa gctttccttt
    48601 caatcatcaa tgtgcatgaa gtggaagggc aaggctttac ttatttgaag ccacagacaa
    48661 gattactgaa aaaactataa gaaaaaagac aaagataggc tatgaggaaa gattccacat
    48721 ccagctgacc tttgtctttt ttagtactat actattttta actaattgtt atgttcactt
    48781 acccggttac ttttcttttg tgtctgcaaa aggatctttc ggttaacttc atcagataat
    48841 ttcaaaaaag catccctatc tccatcttct agtgcttgat cgatttgagc ataaagcatt
    48901 tttagttttg cctcttcttc tttttcttga aaatatcgtt caattaattc agtatcttct
    48961 tcactgactg aagaatccca agaagaaaat ggattgtctt ctaagataga tagatattct
    49021 cttgtctgcc aagcattttc gaatatacat tccacatata aaggcttttt ccaattcaaa
    49081 cggatttcat gaaaaatctg gtctgtgtcc gtaaattctt ttccctgcaa aaacaatttc
    49141 atgggttccc cttcaacaga tagatcactg atctgcagtc ctcgatccgc aaaattcgcc
    49201 ccttcaacaa agtggacatt gcttaaaatc gcttcatgat tggcaagata attcaagatc
    49261 caaaaaactt ctcttcggct aaaagataca tgattgacca tccacgttaa aaaattcttc
    49321 ttttcccgta catcaattag catttttctc acctctctgt taggtcatct aataatgaca
    49381 gtgcctccat atctcctggt tcaagtgtta aataataagt caataattct tgcgctcttt
    49441 tcagctgccc ttcttctctt aagaaaaagg catactcttt caagaaatct ggttcatgtg
    49501 atagttcatg ataagcctgt tcatagtgta cagctgcaac ggtaaagtct tctaattcat
    49561 tataagcatg ggctaggttc cattctgcat aaggattagc agtttcttcc atctgattga
    49621 tcacttgtat cacatcttca tatctttcat catccaagta aagattactt aaagtaagta
    49681 atgtttcatc ttgtttgtct cctacttcta aagctttcag taaaagttct tctgctcgct
    49741 gtctatcatg cagacggaaa gcattttccg aggccaggtg ataaagttcg acttggtaag
    49801 gattttcttt gattccttct tctaacactg tttgtgcttc ttcaatcatt tcttcttctt
    49861 gaagagcttg tcctagatat agatataacg attgataatg cggattaact tctcgaagtt
    49921 cttccagata gttgattgcc tgctgatttt ctttcaattg aagattcgta aaagctagct
    49981 ggaacagtcg atcatctgtc ctttcttctt tcagtgcttc ttctaaaggt acgatagctg
    50041 cttcgaattc tccttgcata cttaaggatt gtcctaaacg ttcctgcatc gagattcctg
    50101 aaatttcatt tatcccgctt gccaacaatg tttcataaac cactgctgcc tctttgaaac
    50161 gatcaactga aaaatacaat tcagctagcg caaactggat caatggttca tcaggaagca
    50221 acccagctgc ttctttcaat tttgcttcac tcacttcagg gattccgata acttggtaca
    50281 aatcagctat cgccaataag ctttgcggat agtaatcact ttgcttatcc actttttcca
    50341 ggtattcaaa agctaagtcg atctcattat cttcgattgc aatttctgct aacgggatgt
    50401 tcagtccgtc attttctggg tattggttga tcagttgttc aaaaatctgt tttgcttcga
    50461 tcaaaaagcc aatagccagc aattcttcgc ctagttcagc taatatatct gctgggtctt
    50521 cttttaatgc tttttctaaa aagatttgtg cttgtgacaa gtcttcatct gataaagctt
    50581 gcaacatttt ttcgctattt gtattcattt caattccctc ttatctcact gacgatttgt
    50641 tgaaataccg tcattgttct caataattct ttttcgtttg gatgttggta tccgtctatt
    50701 atacctattt tttctaaaac aagtattttt ttgctttttt ctgccaaaaa aagaactttt
    50761 tccaaatact ccatctttga aatctgttcc agccagtgga ttggatagcc aagcaagtcc
    50821 agccacacaa aaaagttttt tagttggaaa tcaaaaggat atgcttggag attccacacg
    50881 aaatgaaaca tcattcctaa aaattttttc atactagcag aaagtagatg gccattttcg
    50941 gtcagataaa aagcttgttc aaacagttta ccataatttg acaggttttc tgcctctaat
    51001 tgataaaaat agatcatttc ttcgatcatc ccggcaaacg gccgagtcgt taattgcttt
    51061 ttatttggat agtttttata cagattttgc aaaaaacgat gatcacaaat cattccgcaa
    51121 cagataaaag tcaaaaagtc aatctgcttt ccgtccacct gccgctcgct gattgtttga
    51181 tcataaacga tgcgttctgg cagattgatg gtttgcagca ctacacgatt tggtagctgc
    51241 aaaatcgtcc gctgcggtgt taatgctcgg cttagagaac ggatcgatac agggatcatc
    51301 catagtgatg aatcaagggt tgtatgtttt tgaaaaaaac ctgccagatc catgatccct
    51361 tcatttccaa atccgatgat aaacatggat tgattttcgg gatagcgttt gacgaaatca
    51421 agcaggttct ttaattctgt taaatgattg cattgagtgt tggcacaaat ataccagtca
    51481 ttgtttgctt gatttgcaaa taacgggttg atcttatctg caaacaaatc ataatagcgc
    51541 tgattcgtta aaaataagat tggctgatca atggaaagtt ctcttagctg actgactagc
    51601 gtttctccgt aaaacacttt cgtcaacgca tcttctactt gataatcaag ttccatacgc
    51661 atccctcctc ttcttatttt tattttacca ttagtaagca aattctagaa aagataagag
    51721 caaagctcac ctcataactt gcaaataaat aaaaattaaa taaaatacat gtgttttcta
    51781 tgtatgacat acggattcgg aatctctgtc tcttatttat tccttttctg ctaggaaata
    51841 aaaagaaaag cacttgtagc cctagagcca caagcgcttt ttcaactgtc ttatttaaca
    51901 gcgtctttta atgctttacc tggtttgaat gcaggtactt tgctagctgg aatttcgatt
    51961 tcttcaccag tttgtgggtt acgacctttg cgagctgcgc gttcgcgaac ttcaaagtta
    52021 ccaaaaccga ttaattgaac tttttcgccg ttagctaaag catcttgaat agttgaaaat
    52081 acagcatcta ctgctgcagt tgcatctttt ttagttaaat cagtagctga agcaactttt
    52141 tcgattaatt ctgctttgtt tgccatggat agattcacct cctccacaag agatatgata
    52201 gaaatatcat ttagttaaaa ctttctgagt ggtccagaag tttaaaaaga aatattgatt
    52261 aacttcaata ttctgttatc acgatatcat atgaaccgct taatagcaag gtttttacca
    52321 attttcacat gcttttttta gtcatagaaa cgtaacaaac gaaactgtct ttttcttttg
    52381 aaaaaaaaac gtcgatatgg taaaatgcct attttctcct acgagggata attcggatgg
    52441 acgtcccttc aaaagtaaat gcttttctga tttgattttc taagaaacgt tcataagaaa
    52501 aatgcatcaa ttcctcttca ttgacaaaaa cgacaaatgt aggtggtttg acagcgactt
    52561 gtgtagcata aaaaatcttc aggcgttttc ctttatctgt tggtgttgga ttgattgcta
    52621 cagcatccat aatgatatca ttcaacacag ccgaagagat acgaagattc tggttcatac
    52681 ttacttgttc gatcatttca ggcagacgtt ccaaccgttg tttagtcact gcagaaacaa
    52741 aaacgattgg agcgtaatca agataccgga attcatcacg aatctcttgt tcgaaatcac
    52801 gcattgtatt ggtttctttc tttacaagat cccatttatt cacgacgata ataattccac
    52861 gaccagcttc atgggcgtaa cccgctactc gcttgtcttg ttcccgaatc ccttcttcag
    52921 cattcagtac catcaaaacg atatcagaac ggtcaatggc acgcattgca cgcatcacgc
    52981 tgtatttttc agtattttcg tatacttttc cgcgtttacg cataccagct gtatctatca
    53041 ttaagaattt ctgaccactt tcagaaacga agtgggtgtc gattgcgtct cgggtcgttc
    53101 cttcaatatc cgaaacaatt acacgttctt ctcctaaaat cgcattgatc aaagaggatt
    53161 taccaacatt tgggcgacca atcaaactaa acttaatcgt gtcgtcgtcc tcttcttcag
    53221 tctcttcaga gaaatacttc actgcttcat ctaaaatatc accaatccct agtccatggc
    53281 ttcctgaaac aggataagga tcgcctagcc ctaaggaata aaattcatag atatcgtttc
    53341 tcatttctgg attatctact ttattgactg ctaaaatcac tggtttatta cttctgtata
    53401 aaattttcgc aaccatctca tctgcgtctg tcacgccttc gcgtccgctg actacacaaa
    53461 tgattacatc tgcttcttca atcgcgattt ctgcttgatg cttgatctga tccataaaag
    53521 gttcatcgcc aagatcaatt ccgcctgtgt cgatcacgct gaattctcga cctaaccatt
    53581 caccttttgc ataaatacga tcacgagtta ctccaggagt atcttcgaca atcgagatac
    53641 gctcaccagc gatgcggtta aaaatcgtag acttaccgac gttcgggcgg ccaacgattg
    53701 caattgttgg atttgccata ttatttcctc ctcacaagtt tccaatctca cttagtttaa
    53761 cgaatgcatt gccaactttc aagcatagat tgtcaattaa acgaagtatt tttattcctt
    53821 attgaaatac taatcgcttt tcaaaagaaa aactgagaca aaagcagctg ctttcatctc
    53881 agtcacagat acaaaatcaa ttatttttca gaatcatcgg ttgctttttc ttctacttct
    53941 tctttcaacg catcacctaa aatgtctccc atagtaaatc cagtattttc ttctggtaat
    54001 tcataagact caacattttt tggttcttct tctgaagctg gtttttcttc taaagctttg
    54061 atactcaatg caatacgatg ttcttctgga tggatctcta atactttcac tttcacttcg
    54121 tcaccttcat gaagcacttc atgtggtgtc gcaatatgtt tatgtgaaat ttgagagata
    54181 tggactagcc cttctactcc agggaacact tcaacaaagg caccgaagct tgtcaaacgt
    54241 ttaactgttc cgtctaatac tgtgccaact ggagcttttg tatcgatttc tgaccaaggt
    54301 ccaggctgtg tgtctttgat tgacaaggaa acacgttctt gttcaggatt gatagacaac
    54361 actttgacat ttacatcatc attgacagcc aatacatcac ttggttttgc tacatgacta
    54421 tgtgaaatct ctgagacatg gactagaccg tcaattccgc ctaggtcgac aaatgcgcca
    54481 aaatcagtaa gacgtgcaac acgtccactg agaacatctc cctcatggat agttgaaaga
    54541 atttcttttt tcttttcttc tctttcgttt tccactaccg ctttatgcga taagatcaaa
    54601 cgattttctg atggttcgat ttcgatgatt ttaaagctca atgttttgcc tttatagtct
    54661 gaaaagtctg caacaaaatg atcttccacc attgatgcag ggacaaaacc acgtacaccg
    54721 acgtcaacga ctaaaccgcc tttgacaaca tttgtaacag gtgcttcgat gaccttgccc
    54781 tcttggaaat ctctttcgat atcttcccaa acttttttag catc
//
LOCUS       DAYLZO010000006        48768 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.006, whole genome shotgun sequence.
ACCESSION   DAYLZO010000006 DAYLZO010000000
VERSION     DAYLZO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.006"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            78..1739
                     /locus_tag="ACGVJG_000315"
     CDS             78..1739
                     /locus_tag="ACGVJG_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFJ6556303.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1809..2156)
                     /locus_tag="ACGVJG_000316"
     CDS             complement(1809..2156)
                     /locus_tag="ACGVJG_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFJ6556304.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2240..3181
                     /gene="opp3b"
                     /locus_tag="ACGVJG_000317"
     CDS             2240..3181
                     /gene="opp3b"
                     /locus_tag="ACGVJG_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFJ6556305.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3182..4225
                     /locus_tag="ACGVJG_000318"
     CDS             3182..4225
                     /locus_tag="ACGVJG_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6556306.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4231..5301
                     /locus_tag="ACGVJG_000319"
     CDS             4231..5301
                     /locus_tag="ACGVJG_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556307.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5291..6244
                     /locus_tag="ACGVJG_000320"
     CDS             5291..6244
                     /locus_tag="ACGVJG_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556308.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6256..7266
                     /locus_tag="ACGVJG_000321"
     CDS             6256..7266
                     /locus_tag="ACGVJG_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFJ6556309.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7551..8372)
                     /locus_tag="ACGVJG_000322"
     CDS             complement(7551..8372)
                     /locus_tag="ACGVJG_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFJ6556310.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8469..8747)
                     /locus_tag="ACGVJG_000323"
     CDS             complement(8469..8747)
                     /locus_tag="ACGVJG_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556311.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9015..11024
                     /gene="metG"
                     /locus_tag="ACGVJG_000324"
     CDS             9015..11024
                     /gene="metG"
                     /locus_tag="ACGVJG_000324"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFJ6556312.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11503..12090)
                     /locus_tag="ACGVJG_000325"
     CDS             complement(11503..12090)
                     /locus_tag="ACGVJG_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556313.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12265..13587
                     /locus_tag="ACGVJG_000326"
     CDS             12265..13587
                     /locus_tag="ACGVJG_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFJ6556314.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13742..14518
                     /locus_tag="ACGVJG_000327"
     CDS             13742..14518
                     /locus_tag="ACGVJG_000327"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFJ6556315.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14511..15080
                     /gene="rnmV"
                     /locus_tag="ACGVJG_000328"
     CDS             14511..15080
                     /gene="rnmV"
                     /locus_tag="ACGVJG_000328"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFJ6556316.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15073..15957
                     /gene="rsmA"
                     /locus_tag="ACGVJG_000329"
     CDS             15073..15957
                     /gene="rsmA"
                     /locus_tag="ACGVJG_000329"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFJ6556317.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16408..17037
                     /locus_tag="ACGVJG_000330"
     CDS             16408..17037
                     /locus_tag="ACGVJG_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556318.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17161..18270)
                     /locus_tag="ACGVJG_000331"
     CDS             complement(17161..18270)
                     /locus_tag="ACGVJG_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556319.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18479..18901
                     /gene="mgsA"
                     /locus_tag="ACGVJG_000332"
     CDS             18479..18901
                     /gene="mgsA"
                     /locus_tag="ACGVJG_000332"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFJ6556320.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19088..19186
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19224..19760
                     /locus_tag="ACGVJG_000333"
     CDS             19224..19760
                     /locus_tag="ACGVJG_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFJ6556321.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19800..21563)
                     /locus_tag="ACGVJG_000334"
     CDS             complement(19800..21563)
                     /locus_tag="ACGVJG_000334"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556322.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21566..23281)
                     /locus_tag="ACGVJG_000335"
     CDS             complement(21566..23281)
                     /locus_tag="ACGVJG_000335"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556323.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24060..24284)
                     /locus_tag="ACGVJG_000336"
     CDS             complement(24060..24284)
                     /locus_tag="ACGVJG_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556324.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24475..27252
                     /locus_tag="ACGVJG_000337"
     CDS             24475..27252
                     /locus_tag="ACGVJG_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFJ6556325.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27333..27752
                     /locus_tag="ACGVJG_000338"
     CDS             27333..27752
                     /locus_tag="ACGVJG_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFJ6556326.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27764..28234
                     /locus_tag="ACGVJG_000339"
     CDS             27764..28234
                     /locus_tag="ACGVJG_000339"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFJ6556327.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28251..29030
                     /locus_tag="ACGVJG_000340"
     CDS             28251..29030
                     /locus_tag="ACGVJG_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFJ6556328.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29017..29838
                     /locus_tag="ACGVJG_000341"
     CDS             29017..29838
                     /locus_tag="ACGVJG_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFJ6556329.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            29855..30904
                     /locus_tag="ACGVJG_000342"
     CDS             29855..30904
                     /locus_tag="ACGVJG_000342"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFJ6556330.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            30917..31921
                     /locus_tag="ACGVJG_000343"
     CDS             30917..31921
                     /locus_tag="ACGVJG_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFJ6556331.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32554..33573
                     /locus_tag="ACGVJG_000344"
     CDS             32554..33573
                     /locus_tag="ACGVJG_000344"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFJ6556332.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33616..34497
                     /gene="galU"
                     /locus_tag="ACGVJG_000345"
     CDS             33616..34497
                     /gene="galU"
                     /locus_tag="ACGVJG_000345"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFJ6556333.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            34797..35174
                     /locus_tag="ACGVJG_000346"
     CDS             34797..35174
                     /locus_tag="ACGVJG_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556334.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            35780..36394
                     /locus_tag="ACGVJG_000347"
     CDS             35780..36394
                     /locus_tag="ACGVJG_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFJ6556335.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            37167..38906
                     /gene="yfcC"
                     /locus_tag="ACGVJG_000348"
     CDS             37167..38906
                     /gene="yfcC"
                     /locus_tag="ACGVJG_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFJ6556336.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            39063..40247
                     /gene="iadA"
                     /locus_tag="ACGVJG_000349"
     CDS             39063..40247
                     /gene="iadA"
                     /locus_tag="ACGVJG_000349"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFJ6556337.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            40358..40561
                     /locus_tag="ACGVJG_000350"
     CDS             40358..40561
                     /locus_tag="ACGVJG_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFJ6556338.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(40662..41324)
                     /locus_tag="ACGVJG_000351"
     CDS             complement(40662..41324)
                     /locus_tag="ACGVJG_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556339.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(41337..42839)
                     /locus_tag="ACGVJG_000352"
     CDS             complement(41337..42839)
                     /locus_tag="ACGVJG_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6556340.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            43387..45066
                     /locus_tag="ACGVJG_000353"
     CDS             43387..45066
                     /locus_tag="ACGVJG_000353"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFJ6556341.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            45193..45672
                     /locus_tag="ACGVJG_000354"
     CDS             45193..45672
                     /locus_tag="ACGVJG_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFJ6556342.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            45891..46571
                     /locus_tag="ACGVJG_000355"
     CDS             45891..46571
                     /locus_tag="ACGVJG_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFJ6556343.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            46579..47910
                     /locus_tag="ACGVJG_000356"
     CDS             46579..47910
                     /locus_tag="ACGVJG_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFJ6556344.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            47925..>48768
                     /locus_tag="ACGVJG_000357"
     CDS             47925..>48768
                     /locus_tag="ACGVJG_000357"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFJ6556345.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRH"
BASE COUNT        15870 a         8560 c        10132 g        14206 t
ORIGIN      
        1 ataattattc attaaactct taaacaactt cagaaaatat taattattct gaaaattcca
       61 ataaaggggt acgtaaaatg aaaaagaaat ttacatttgg tttggttgcg gtatgcggcc
      121 ttgtacttgc tggatgttat ggcggcagtt cagccacaag cagtagttca gcaaaaagcg
      181 gttcagcaga tggaggaggc gtatttaact tagtcgtgcc tcaagaaatg cctacagctg
      241 acttatcagt tgcaacagat acaatcagtt ttacagcatt gaataacgtt tatgaaggaa
      301 tttaccgtct tgatgaagac agcaaaccac agccggcagg agctagcgag ttggcagaag
      361 tcagtgaaga cggattaact tacaaaatca aattacgaga agacgcaaaa tggtcaaatg
      421 gtgatcctgt tacagcagcg gattacgttt atggctggca acgaacagta gacccagcta
      481 cagcttctga atatgcttac ctgtatgcgc cagttgaaaa tgcagaagac attacagctg
      541 gaaagaaaga taaatcagaa ctagggatca aagcagttgg tgactatgaa ttagaaatca
      601 aattgacgaa acaaacacca tatttccaat atctgcttgc tttcccatca tttttcccac
      661 aaaaacaatc cgtagtggaa gaaaatgggg atgcctatgc atctgcaagt gataaagctg
      721 tttataatgg accattcaca ttagaagatt ttgatggacc aggaacagat acagaatgga
      781 cttataagaa aaatgatcat tactgggaca aagatacagt aaaactttct gaaatcaaag
      841 tcagtgtagt aaaagaatct tctactgcat tgaacttatt caaagatggc caagcagatg
      901 acgtgatctt gtctggtgag ttagcacagc aaaacgcaaa cgatccagct tatacttctg
      961 ttaaagaagc gcggacaagc tatatcgaat tcaatcaacg tgaaaagaat tcaccattca
     1021 acaatgtgaa cctaagaaaa gctatttctt attctatcga tcgtgaagct ttagtaaaac
     1081 aagtactagg tgacggatca gttgtctcta caggattgat cccatcagat atggtaaaaa
     1141 gtccaacaac gaacgaagac tttgcaaaag aagctggaaa attagtttct tatgacaaag
     1201 ataaagcaaa agaatattgg gaaaaagcga aaaaagaatt gggcgttgat tctttagaat
     1261 ttgatttgat ggcttctgat gatgattcat cgaaaaaagt aatcgaatat gttcaaaact
     1321 caatccaaga aaacttggat ggcgtaacag tcaaaccaac acctgttcca ttctctgtac
     1381 gtcttgaccg ctcaacttcg ggtgattttg acgcagtact aggcggatgg gctgctgact
     1441 atgcagaccc aagcagcttt actgatttgt ttgtcacagg taattcatac aaccgtggtc
     1501 gctggagcaa tgcagaatat gacgaagcag ttaaagcagc ttcatcaaca gacgctggcg
     1561 atgaacaagc ccgttgggat gatttccaaa aagcaatcaa gatcatctct gatgatatgg
     1621 gtgtcgttcc ggtgtaccaa aaagcagaag gtcacctagt gaatgaaaaa atcaaaggta
     1681 tcgttcacca tgcggcaggc gcttcatggg attacaaatg gacatacgtt gaagaataat
     1741 cgttaatacc gtgagcatga gaaaaaacga ggctgcggca aatgtcgcaa cctcgttctc
     1801 atattcactt atcctaacaa agaaaaaagc atgaaaatag cagaaagagc aattagtatt
     1861 ccggcaatga tcagccaaaa gctgtacata ccgtagctgg cagacgagag cgtggaaaat
     1921 tctggttttt tctttcggtt ccttgttcgt gcaagatgga ccgatcgctg aaaatgatcg
     1981 aagaaacccg aagaaaagac ccataaaaaa ccgccgataa tcagaaacgg caatgaccat
     2041 aagaatagat cattagataa ttgaatgagt gtaaaggaat ggttgatgag attttttatt
     2101 aaaataacca gaatcagtag aaaagctact gcgtagggga tagatttttt tttcatttga
     2161 ttgctccttt atttttggtt cattatctaa taatgtagcg aaacatctgt cagaagtcaa
     2221 aagctagggg gaaacattag tgaatagtta tatcaaatat gtattaaaac gtgtcttttt
     2281 catgatcatc acgctttggt taattgcaac aatcaccttt ttcttgatgc aattgctgcc
     2341 gggcacgcca tatacgaatc aggaacgttt gagtcctgaa acaatcgaga tgctaaataa
     2401 acaagtcggt ttggataaac cagttatcgt acaatatggg atttatcttt ctaatttgct
     2461 tcaaggagat tttggaattt ccttccaatt taaaaaccag ccagtcgcac atttgttagc
     2521 tggaagaatc ggaccatcct tgcagttagg gttacaggca attatctttg gaacagtcct
     2581 agggacgatt ttaggaacga tctctgccat gaagcaaaat acatgggcag atacatcttc
     2641 tacactcgta gcgatattag gtcgttccat tccaaacttt gtatttgctg ttttattaca
     2701 atatatcttt gcaatcaaat tacaagtttt gccaatcgca aaatgggatg gctttgtgta
     2761 cacaatctta ccaacaattg cgttggccat gtcgccgctt gctgactctg ctcggtttat
     2821 ccgtacggaa atggtggaag tactgcatag tgattatgtg gaattagcaa aagcaaaagg
     2881 attgagccgt tgggaaatcg cctttaaaca tggtcttcga aatagtttga ttccattgat
     2941 gactttatta ggaccgttag ctgtagcatt gatgaccgga tcactggtgg tagaaaatat
     3001 ctttgctatt cctggtatcg gggaacaatt cgttaagtca atcacaacaa atgattatcc
     3061 tacgatcatg gctgtaacga tcctttactc atttatgctg atttttgtta ttttgatcgt
     3121 ggatctattg tacggtctag ttgatccaag aattcgtgtt tcagaaggga gccgaggata
     3181 aatggaaatg ataccaaagc gttatgaaac agttgcagat atccctgcgg atgaatttct
     3241 tccattacaa aaaaataccg aagaagaacg tgaacagatt actgcacctt ctttgaattt
     3301 tatccaagat tcatggcgcc gtttaaagaa aaacaaagct gccgttattt caatggtttt
     3361 actattggtc atcattttga tctctgtgat cacaatcttt gtttctcctc atgatccaac
     3421 tgcccaaaac gtggcttata tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg
     3481 attgaatgga aaagcaatgg ttggtggtga acttgttgat aaatatgctc aagcaaacgt
     3541 cccttcaaac gtaaatttct ttttaggaac agacggtttg ggacgtgatg tattaagccg
     3601 cttatttatg ggaatgagaa tctcgttgct gatcgcattt attgctgcat tactagatgt
     3661 aacgattggt gtagcttacg ggttggtttc tggtttgtta ggcggacgtg tcgataacgt
     3721 gatgcaacgt tttttggaaa tcttatcagg aattccaaac ctcgttgtca tgattttgat
     3781 gttggtcgtt tttgaaccag gaattttttc aatcgtggct gctatggcca tcacaaactg
     3841 gataccaatg gcacgtatcg ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt
     3901 tttagctgga ttgacattag gtgaatcaag aatgaaaatt gcattcaaac atattttgcc
     3961 gaatatctct agtgtcatta ttattcaaat gatgtttagt attccatctg ctattttctt
     4021 cgaagccttt ttaagcttta tcggtttagg tcttacgcct ccgtcagctt cattaggaac
     4081 gatgttgagc gaagggtaca agacattcct gtatctgcct tatctattat ggattccggc
     4141 agtaacttta tcggttatca tgattggctt taacttactt gctgatggat tgcgtgatgc
     4201 ctttgatcct aaaatgaaag agtgaagacg atgacgaaaa ttttagaagt gaaagactta
     4261 gaaatctctt ttgatacgta tgctggaaag gtacgagcga ttcgcggtgt caattttcat
     4321 ttgaataaag gagaaacatt ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg
     4381 accagaagca tcatgcgctt gctgtcaagc aacgccaata tagatgctgg tcaaatcctc
     4441 tttaaaggta acgatatcgt tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa
     4501 gaaatagcga tgattttcca agatccaatg acctctttag atccgacgat gcccattggg
     4561 aagcaagtcg ctgagtccct tagaaagcat aataaagttt caaaaaaaga gggacaaaaa
     4621 gctgcattag aattgcttaa attagttgga atacccgaag ctgagaaacg tatcaacagt
     4681 tatccgcatc agttctcagg aggtcagcgt caacggatcg tgatcgcgat tgcactggta
     4741 tgttatccgg aaatcctgat tgctgatgaa cctacaacag cgcttgatgt gactattcaa
     4801 gcacagattt tggagctgct gaaagaaatt cagacaaaaa tcgatacatc aattatcttt
     4861 atcactcatg accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga
     4921 ggaagaatcg ttgaagttgg aacatcggaa gaaattttct acaacccgca acatccctat
     4981 acttggggac tgcttggttc aatgccgaca ttagatagtg cgaacgatcg attgtatgct
     5041 attcctggtt ctccaccgga tttattgaac ccgccaaaag gagacgcatt ctatccaaga
     5101 aatgaattcg cgatgaaaat cgatgcagaa caagaaccac cattttttga actatcaaaa
     5161 acacacaagg cagcaacttg gctacttgct ccgcaggcac ccaaagtgac accgccagaa
     5221 gaaatccaac gtcgctgggc tatctttaaa gagaaacaaa aacatactta cggtcaagga
     5281 ggaattgctg atgccaaata aactacttga agttaaagga ttgaaacagt atttcaatgt
     5341 aggaaaaaag aatgaagtac atgctgtaaa tgacatcagc tttcacattt atgaaggaga
     5401 aacattcggt ttagttggag aatctggaag cggaaaatcc actactggcc gtacgatcat
     5461 tcgcttgaat gaacctactg atggtgagat attattcgac ggtcaagata taacgaagat
     5521 caaagataaa aaaggtttga cgaaattccg gcatgatgtt cagatgatct ttcaagatcc
     5581 atacgcttcg ttgaatcctc gtatgaaagt cagagatatc attgccgaag gaattgatgt
     5641 taatggacta gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac
     5701 tgttggattg aatccaagcc acggtactcg ttatcctcat gaattttcag gtgggcaacg
     5761 ccagcgtatt ggtattgcac gagcattggc agtaaaaccg cgttttatca tttgtgatga
     5821 accaatttct gcactagatg tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct
     5881 ccaaaaagaa catcaattga cctatctatt tattgcacat gatttgtcga tggttaaaca
     5941 tattagtgac cgaatcggtg taatgcataa cggcttgtta ttagagatgg gaacaagtga
     6001 tgaaatctat aatcacggtg ttcatcctta tacagaaagt ttgttatctg ctattcctct
     6061 accagatcca gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg aaccagatga
     6121 cggacaagta cgtcaacttc gtgaaatcgc accagaacat tttgtctatg caactgagca
     6181 ggaagtccca tattacgcaa aaaaactaaa acgacaaaag gaatctcttc tggttgctaa
     6241 ctaggaggga catagatgct aaaagctttt aaattccgta tgtatccgac agaagaacaa
     6301 aaacagcaat tgattcgtac ttttggatgt gctcgattta cctataacca tttgctgaaa
     6361 aagcggcaga aaagttggca acaaacagga gtagccaatt tttcgctcac acccgctaca
     6421 ttaaaaaaag aatacccatt cttaaaagaa gtagacagtc ttgctttagc aaatgcacag
     6481 cttaatttgg atcgggcttt tcgcaattat ttcaagggac gtgccagctt cccaaagctt
     6541 aaaacgaaaa aaagtatgtg gcaatcttat acgaccaaca accaaacccg tacgatctat
     6601 ctggaaaatg gctacttaaa gttaccaaaa caaaaagaac taatcaagat aaatcagcat
     6661 cggccagttg aaggatcgat tcgctccgct acgatttctg ctcgatataa tgaagaattt
     6721 tatgtcgcac tactttgtga tgtttcgcca gtaaaaaaag aatctttagc taaatggatc
     6781 gggatcgcct atcatccgaa gacattgatc caaacatctc gacctctaga agtaacttta
     6841 ccaaaatttc accagacaga agaaaagctg caacatgcac agcggaaatt gaatgtgaaa
     6901 gtccgctccg ctcaccatag aaagatcagg ttagatcagg caagcaatta tcagaaacaa
     6961 aagagaaaag taatggatct ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa
     7021 caattatctg ggaaattggt caaacaatat gactacttat ttgtagaatc ttttccaaaa
     7081 gaagaggcac atgctgattt ttctattcac gactggcata aattaataac aaaattgcgt
     7141 tataagtccc aatggtataa taaaaaattt cttttgatca ataccgacgg agcagaagaa
     7201 agcaattccg taagaaagag ccaagtagta gaaaaaatgg gcagacattc ggttatcaag
     7261 ggataaaaaa tgaatgaaac tgataggagc gttagggtta gccttgttaa taaaaagtct
     7321 taatgacttt tgtttctagg aatttttctt ttgaagaaaa gctcactaat tatgacaaac
     7381 caataaaaca atagcagaaa aagttcgtac gagctttttc tgctattgtt aattttttat
     7441 tcagaaagtt tacgaataca tcaaaattct gaaacaaaaa acgtaactca caaagagttg
     7501 tttttgcgtt ttttcttgaa tgattaggtc tgaatctttt gctcacaacc ttattcatgg
     7561 tgccccggct tgagctgata gatttttgaa ggacggccga ttcctacagg acgttcaccg
     7621 atctcatcga aatattggag catagctttc ttaaaattgg aatgatcgat cgttttataa
     7681 tcgatcccta agaatttagc aaatactttt cgagcttccg taatcgtgaa atctttacct
     7741 aaaacttgta gaacttgcgg ctcgtgttcc attttattga ctacacggtt gaatgctttc
     7801 aagatgattt gactatggtc aaaagccaaa gtatctttgc cagaagactc tccagtgact
     7861 aaatcaagcg agatagccac ttctccgcta gataaatgga tcttgttact atgacgttct
     7921 agtgtgaacc agcgtacttc cttggcatca tctccggcga ttagcggttc ttctccaata
     7981 aaggctaagt agctgactgt gacgacccag cctcgcggat ctcgattagg cgtactgaat
     8041 gtatgcagtt gctcaatatt ttcttttgaa ataaccacac cagtttcttc ctttgtttct
     8101 cttaagacgc tttctccagt tgactcatcc ttttggacaa agccaccagg caatgcccat
     8161 gaatttcgat aaggatgccc ttttctttgg atcaacaaaa ctttcagctg atcttcttct
     8221 ttgttatagc acatcagtac gatatcgaca gtcaaagagg gcttgtcata ttctggacgt
     8281 tcctgttttt gataccagtt aagaaattct tcttcacttg ctttatgttc ataatataat
     8341 ttttcttctt ctttagaagc aaaacggttc atctgaccac ctccattgac agtaaaagta
     8401 ctttttacct tattgtagca gactctgaaa aaaaaccaag gatttacttg aaaaatctag
     8461 gttatcattt aaatatgaaa aataaatgga agtatgagat tgaataaaaa gaaaagcaga
     8521 taaaacggca atactttata ccgctcttca cgaatggcaa agaagccaca aataacgata
     8581 tatacgtcaa tgataaaacg caaaatccaa gctgctgtca taaaactgtt caaatgccca
     8641 tggaggttaa aataaccata catacgaata taaaaatagc tgaatattaa atgtgctaat
     8701 gaaagaaaag caatactaag tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc
     8761 ccttactaag atagatagtc tttattatac cacgtgtgag gggagaaata aaaagccgtt
     8821 ttttagtaga tatgaaacaa aacaaaaacc cgcatttatt caacctattc atccttttaa
     8881 aggatagatt gaacgtagat agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc
     8941 taacagcatg ctttttttat ggtatactag caaggaaacg aattcgcatc atgcgataaa
     9001 ggaggaagag ccccgtggca gaaaaggaaa ctttttatat cactacgcca atctattatc
     9061 caagcggcaa acttcatatc ggcaactcgt atactacgat tgcatgtgac gcaattgctc
     9121 gttataaacg gttgatgggc tttgatgtgt tttatctcac tggagtggat gaacatggtc
     9181 aaaaaatcga aacaaaagca gaagaactag gtgtaaaacc gcaagaatat gtagataaaa
     9241 tggcggctga tgtcaagaaa ttatggaaaa cgcttgatat cagttatgat aaattcattc
     9301 gcacgacaga tgattaccat aaagcagctg ttcaaaaaat atttgatcgt ttattagaac
     9361 agggagatat ttacttaggt gaatatgaag gctggtactc tgtttctgac gaagaattct
     9421 ttacagaaac acaacttgca gaagtgtaca aagatgacga aggtaacgtg atcggtggta
     9481 aagcgccaag tggacacgaa gtagaattgg taaaagaaga atcatacttc ttccgcatga
     9541 gcaaatacgc agaccgtcta ttggaatact atgaagaaca tcccgaattc atccagccag
     9601 aatctcgtaa aaacgaaatg atcaacaact ttatcaaacc aggattagag gatcttgcag
     9661 tttcacgaac aacttttact tggggagtac cagtcaagag tgatccgaaa catgtggtgt
     9721 atgtatggat cgatgcatta tccaattata ttactgcact aggatatggc tccgaagatg
     9781 aaagtctatt cgaaaaatac tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc
     9841 gctttcatac gatctattgg ccaatcatgc tgatggccct tgatttgcct ctacctaaaa
     9901 aagtatttgg gcatggctgg ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa
     9961 atgtcgttta tcctgaaatg cttgtggaac gttatggttt agatgcactc cgttattatt
    10021 tgcttcgtgc tgttccgttt gggtcagatg gtgtctttac gccagaagat tttgtttccc
    10081 gtgtcaatta tgatctggca aatgatctag ggaacttatt gaatcgaacg attgctatga
    10141 tcaacaaata ttgtgatgga ctcgttcctg actatgcctc acttgtcact ccatttgata
    10201 gtgaactgtc tacgacggct gctaacgttg tcggacgtta ccatgatgca atggaaaaaa
    10261 tggaattcaa tacagcattg gctgaaatct gggtgctgat ttctcgtgca aacaaataca
    10321 ttgatgaaac agaaccatgg gtattagcaa aagacgaaga aaagaaagct gaattgaata
    10381 gtgtcatgat ccatttagct gaatctttgc gtatcgcagc gatattactt cagccaatca
    10441 tgacagaaac gccagagaag atcttcaatc aactaggatt ggactctgaa acaatgaatc
    10501 ttgaaggatt acactttggc gaatttcctt caggaacaaa agtagttgca aaaggtacac
    10561 caatcttccc acgtttagat atggaagaag aagttgcttt tattcaagaa aaaatgtctg
    10621 aaggtactca aacaaacgaa gatacagtca aatgggatcc agaagaaaca gaacttgttt
    10681 caacgaaaga aaaacaaatc aaatttgatg tatttgaaaa agtagaacta aaagtagcag
    10741 aagtcatcaa ctgtcaaaaa gtagaagggg cagataagtt gctgcaattc cgactagatg
    10801 ctggagacag ccaagaccgt caaatcttgt caggaatcgc tgaattttat cctgatccaa
    10861 gcgaattgat cgggaaaaaa gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg
    10921 gacaaatcag tcaaggaatg atcctttctg cagaagcacc agatggctct ttacaagtaa
    10981 tcgaagcacc aaaatccatg ccaaatggct cagaaatcgc atagaaattc aaagggggta
    11041 tgacactagt tgtgtcatac cccctttttc cgaaatttga gaagcaattt tcgtaaattc
    11101 cttcttatta cttgaagcca atcatataat acagaaacag agaactatcc aaaataaatc
    11161 ctagaaaaag aagtacgagc ttcgctgatt tttgttaatt tagaataata ctaataggaa
    11221 tcgtaaaaac agaaaaaaag cataatgata aagggtcggg cagattttaa ttgattcatc
    11281 tggtactctt ttttattgtc cttacattca taaagtttga atgcaatgac tttattacat
    11341 ctatatttat atctaacagt agcgttgatg tgttaattat aagttctatc actttatgtt
    11401 caattagttt gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc
    11461 tttgacttcg atgattgttt tctttatttt gtaaaaatcc tatcacttga ttttatggta
    11521 accttttatt tttgtcttca aacggcttgg taagagagca gtaagcagtg caaatgggat
    11581 cgaaaagaca taccagaaaa tcgtacgtcc gataccataa tgttgataga gctttgtcaa
    11641 catcacgtgt aacaagatca accctaaaag caaggagcca gctataaacc aatgattatc
    11701 ccaatgcatg acttcactaa gtgccatcac tccaaaagga tagaaataaa aatagatccc
    11761 gactaaggtc atccgccagt tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc
    11821 tgaaagcaaa ataccgatag gcggaaaaag cccaaccaaa aaggaataca acatccccca
    11881 caagatcatc acaggacctt tgatcaatgc aacgattgcg atgaacaaag tcagcaatat
    11941 tagtttttgt gataacgttg caacagtcat taatccaaat aaaattaatg tgaaccaatg
    12001 ccaatgggac tgctgttgtt ctttacggga aaaatcgacc cggttggata aataatctgc
    12061 taatttcgga taagaacgct taaaatccat aaactgttcc tccttcgtgg aaaaggtagg
    12121 tttagtatac gaaaaataaa aaagaaatac atccgtctta agagggagtt tttcactgaa
    12181 agtggagaaa aaagaatggt aaattaaaat attctcagtt atactgaaaa ttataccaaa
    12241 tttcaaagaa gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat caatctgttt
    12301 ggcgcattag ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt caaaactgct
    12361 gctgttactg ctagtactca gtctacaagt ttgacattgc tagcttggct tttgggtggt
    12421 ttcttaacga tctgtgctgg attgacagtg gctgaattag ctacagcaat cccggaaact
    12481 ggtggagctg taaaatatat tgaagctgcg tatggaaaat tgcccagctt tttattagga
    12541 tgggcgcaaa gcttgattta ctttcctgct aatattgctg cattatcgat tatctttgcg
    12601 acacaattga cgaacttatt acaattatct accgattatc ttttgttgat tgcagtgatc
    12661 accgctgttt ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa
    12721 tcagtgacgt tgattgttaa attaatacca attgctgtga ttgtcatatg gggattattg
    12781 accccaggac aaggaactgt ccagttattt ccaatcgaag ctggaaaaga tgttactttt
    12841 gtcgaaggat tgagcagtgc gttgttagca acgttgtttg cttatgacgg ctggttaggt
    12901 gtcggtgcaa tggcagggga aatgaaacgg ccggaaaaag atttgcctaa agcaattatt
    12961 cttggtttga gttttgtaac agtcgtttat ctattgatca attttgtttt tttgaaaact
    13021 ttgccgattg atcatcttgc cggtaatttg aatgcagctt ctgaagcatc agacgtcatt
    13081 tttggaggaa tcggtggaaa gctggtcact attgggatct tgatctcggt atacggtgca
    13141 ttgaacggtt atacactaac tgggatccgc gtgccttatg cgatggcttt agaagacgag
    13201 ctacctttca gtaaacagct gacaaagctt tcaaagaagt ttacggttcc ttatgttcct
    13261 gcggttttcc agttagcagt agcttgtatt atgatgagcc ttggttcttt cgacttttta
    13321 acagacatgt tgatttttgt gatgtggcta ttcagtttac tgatttttat cggtgtcttt
    13381 attttaagaa aaaaagcgcc ggaattgcca cgtccatata aagtaccact ttatccaatc
    13441 gttccgatta ttgcgattct cggtgctatt ttcattttag gtatgacgat gatgacgcaa
    13501 acgaaattag cattgatcgg tataggtgtt accttgatag gtatcccagt ctattatcaa
    13561 aaaaagaaaa aacgctcaga agagtaaaat atttcaacaa cagacagaaa aagtacgaac
    13621 taggtcctct agatcttcgt gctttttcta tttcaataaa acaagttctt ctattttgat
    13681 ttttgctact tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa
    13741 catgatattt gattctcata cccatttaaa tgctgaacag ttcaatgaag atattccaga
    13801 aacgattcag cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac
    13861 gccaacgatt gaaaagtctt tgcaattaag ccatgactat ccaaatatat acagtattat
    13921 cggctggcat ccgacagagg ctggcagtta tacgaaagaa atcgaaaata aattacaaga
    13981 acaattgact ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt atcattggat
    14041 ggaagacccg aaagaagtcc aagataaagt atttcgcaga cagattgcta tcgcaaaaga
    14101 aatgaaccta ccaatcagta ttcatactag agaagcactt gaagacacgt atcaaatcct
    14161 aaaagaagaa gatgttcgag atatcggtgg aatcatgcat agtttcagtg gtgattatga
    14221 atgggcaaaa cgatttcttg acttagggat gcatatttca tttagcggtg tcgtcacttt
    14281 caaaaaagca ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac gattacttgt
    14341 ggaaacagat gccccttatc tggcaccagt tccttttaga ggaaagcgaa atgaaccagg
    14401 ctatactcgt tatacagtag aaaagattgc tgaacttcgg aatcttccga cagaagaagt
    14461 ggctgcccaa acatggacaa atgcgcaccg attatttggt ctaagcgaac atggataaga
    14521 tgaagatcca agagattata gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag
    14581 tagttgatgc agatacgata gaaacaatag ggtctgcaat caatgaagag atcctgatgc
    14641 aaattgaaca tgcccaagag actcgtggag tgatcgtttt taccgatcct gatttttcag
    14701 gagaaaaaat aagaaaaatc attatggaag ctgttcctca ggcaaaacat gcttttttgc
    14761 cgcgcacaca agctaaacca aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg
    14821 ctgctatttt agaagcattg aaaaaggtcg tgacgccaga catttcccaa acggctgttt
    14881 ctgaaatcac tagacaagac cttttagact atggtttaat agctggggca caagcaaaag
    14941 aaaaacgaga aaaactaggt gacgagttgc gcatcggtta cacgaacggc aaacaattgg
    15001 aaaaacgttt gaagatgttt cgtattacgc cgaaagaact cgcccaagca atgaaaatag
    15061 tggaggaaca acatgagtga atatcgtgaa attgcgacac cctctagaac aaaagagatt
    15121 ttattgaaac atggtttttc atttaaaaaa agtttaggac aaaattttct gactgaacca
    15181 aatattttaa ggaaaatcgt agaaactgcg caaatagatg aacatacgaa cgtgatcgaa
    15241 gttggaccag gaatcggtgc attgacggaa caattagcca agcatgcaaa acaagtcgta
    15301 gcatttgaga tcgatgatcg tttgatccct gtattggctg atacgatgca accctatgac
    15361 aatgtgacta ttgtacatca agatatttta aagaccgatt tgtcaacagc tgttagagaa
    15421 acatttcatg aggagcttcc gttaaaagtc gtagcgaatt taccttacta tattacgaca
    15481 ccgatcatga tgcattttct tgaatcagat ttggttgtgg atgaactagt cgtcatgatg
    15541 caaaaagaag tagctgatcg tatctctgct gaaccaggaa cgaaagctta tggttcatta
    15601 tcgattgctg ttcaatatta tatggaagcc agtttagcat tcatcgtacc aaaaacagta
    15661 tttgtccctc agccaaatgt agattcagcg attttgaaac tgacaagaag agatataccg
    15721 gcagtcgaag tgacagacga aaaagcattt ttccgattga caaaagcggc atttcagcaa
    15781 agaagaaaaa cgctttggaa taacttgcag cattcgtatg gcaaagatga ccagacaaaa
    15841 gcatggttag caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg tggggagact
    15901 ttgtcattac aagaattcgc cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa
    15961 aagaggttgt gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa
    16021 acaaaaatag ttcctcatgt ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt
    16081 tgaacaccgt ttaagaaaga ggttgtgggc ctgccgttta actctaagtc acaaggaaca
    16141 atttcaaaaa acagcttctc atgtttttga gttagttggt cttattactt gatgcaagct
    16201 tttgaccact attatgccga acaaggagat gtgacacaaa ttttgtcaca tctcctattt
    16261 ttgtttcttg atcaaaaaat tcttatactt gatactcaca ctcattttgt atggatttag
    16321 aaaagaatat gtgtcttgac agttattagt aagtgagtta tactaagtcg acatttatgt
    16381 gtcaagacat attaggagag agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt
    16441 tctgctttat tgattgggat gggggtcatt atcccaatgg tcatgcctaa aatcatgatt
    16501 ccaccagctt cctttacttt ggcgtctcat gtgccattat ttattgcgat gttttttaca
    16561 ccaggagttg cagtggctgt tgcgctaggt acgacatttg gtttcttttt aacaacgccg
    16621 gttatcatcg cattgcgagc attgtcacat cttgtgtttg cgttgattgg ggcttggtac
    16681 ttgcaaaagc atccgagtat cgttttaaaa aatggacagt ttacttttgc taattggcgt
    16741 ttccaagggt tcaattttgt gatcggtgtg attcattctt tagcagaaat gctagtagtc
    16801 agcatctttt actttattgg taatatgcct gaaacatatt attctcaagg atatttttat
    16861 acagtattca tcttgatggg gattggtggg cttatccaca gtttagttga ttataatatt
    16921 gcttatttta tagcaggaac actcagcaaa tattttgata tccccgtatt tacggcagca
    16981 aaaaaagaaa atcggaaaga gtcgattcaa gggtacgcga agaaaacgac atattagaca
    17041 tgatctctca aaaaaaaaac tctcatctag taatggataa taaatcctct aagatgagag
    17101 ttttttgtcc taaaaagagc tgcaccaaca tcttctggca cagctctaac agggggcaat
    17161 ttatggaagt ttgatgactt gttctgtttc tttatcaaag aagtggcctt tgctgatgtt
    17221 gaaggcaagg tcgactattt cgcctggttt atggaaatct cttgcatcta cttttgagat
    17281 gaactcagta gaaccagttt ttgtgtaaag catcgtttca gctccaagta attcggaaac
    17341 gacaacttct gatttcacta cggcttcagg tgaagcttca agggctactt gttcactgtg
    17401 gatgtcttct ggacgaatac cgaagataac ttcttttcct tcataacctt tttcaactaa
    17461 tactttgttt tttccttcag gaatacgaag cttaagccca tgtccatctg tgataacacc
    17521 atttttcaat gtcacattaa agaagttcat ggcaggagat ccaataaagc cggcaacgaa
    17581 catattgacg ggtgtatcat aaacttcttt cggtgtaccg atttgttgga taaatccgtc
    17641 tttcatgata acgattcggt cagccatagt cattgcttct gtctgatcgt gagtaacata
    17701 gatggtcgtt gtttcaagac gctggtgaag tttggcaatc tcagcacgca tggctacacg
    17761 taatttagca tctaaatttg ataatggctc atccattaag aagacttttg catcccgaac
    17821 gattgcccgt cccaatgcca cacgttggcg ttgtccacca gataaagcag ctggttttcg
    17881 tttcaagtat tctgtcagac ctaagatctc tgctgcattg tccacacgtt ttttgatgtc
    17941 tgctttatca tatttacgta atttcaagcc aaaagccata ttatcaaaaa cagtcatatg
    18001 aggatacaat gcgtagtttt ggaaaaccat ggcaatatca cggtctttag gtgccacgtc
    18061 attcatgact tttccgccga tatttagttc tccttcagag atatcttcca gtcccgcgat
    18121 cattcgcaaa gtagttgact ttccacaacc ggaaggacca acaaaaacga taaattcacg
    18181 gtctttgatt gatagattaa aatcagttac agaataattt tctgcattgt catatttttt
    18241 atatacgttt ttcagagcca tttctaccat aatacgttcc acactttctt ttttagtttt
    18301 attaactatt taaagtataa atgaaagcgt taactaactt caatgtcatg ttgaacaaga
    18361 attttttctt ttttttgtca atgtgctagt tttaagcttt aataaatatc cataagaaat
    18421 aaaaactgat acactggagt aaataagtat gttaacaata tcaaaaggag tgtctcttat
    18481 gaaaatcgct ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat tagcaacagc
    18541 gtataaaaca atattggaaa aacatgaact ttttgctacc gggacgactg ggcagaggat
    18601 cacggaagca accggcttgc cgatccatcg gtttaaatcc ggaccgttag gcggggatca
    18661 gcaaattgga gccatgattt cggaggatca gttagatttg gtgatttttc ttcgagaccc
    18721 gctggctgca cagccccatg aaccagatgt aacagcactt atccgtttat ctgatgtcta
    18781 tgagattccg ttagctacga atatcggaac agctgaggtg ctactaagag gactggaagc
    18841 cggcttccta gattttcgaa gaatcgtcca tgaaatagac aagcatccac tttcattttg
    18901 atggataagc gtatggaaaa ataacgttta atgttcgcct atatgtagct ataaaagcta
    18961 aacaaactga taatatgcga tataatacga acgtattgga taatataaac aataatatta
    19021 gtttttcgta tttgcagtaa atgtgaaaat atgctatagt ttctgtggga aaagatccca
    19081 aaaataatac agagtagaaa ataaagcgtt aagtgctaag aggatgggat gttgcctctt
    19141 ggacgaagga cgtaatgttc gcggttttat tttcgcattc gctgcatgat acgatgtagc
    19201 agctcttaaa ggagatgcta aaaatgaaga aacaccggtt agacgcacgt atgattgcta
    19261 ttatgggctt attgattgca ttgatggtca ctttgtcccg actggtagca atcgagaccc
    19321 catttattaa aatcagtgtc acatttattc cacaagtgat catgggcatt ctttttggtc
    19381 ctttttggtc agggataggc gcagttttag ctgatttagt aggaatggcc ctcttctcga
    19441 aatctgcatt ctttattgga tttacattga atgcatttat tgaaggagca atctacggat
    19501 tctttttcta tcgaaaagag atcacttgga aaaatgccat tctggctacg cttagtgtca
    19561 cgctgatcat caacttattt ttgacaccat tatggcttgc gttgatgtac catgtgccat
    19621 tgttcagctg ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc
    19681 aatcaattgc catttattac gtaggacgta gtattcctta caaaaaaatt ttaagaagtt
    19741 tagcgatcca cgctaaataa aaaacaagca gtcttcccat cccagaagac tgcttgtttt
    19801 tattgtgctg tgaattgtgc ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc
    19861 tcatcatggg tgcccatttc gacaatagct ccctgatcca tcactaatat ctggtccgca
    19921 tttttgattg tggacaggcg atgggcaatc acaaaactgg tttttccttc catcattgct
    19981 aagaaggctt gttggatttt tttctctgtc aatgtatcga ctgagctggt agcttcatct
    20041 aagatcaaca tagggggatt actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt
    20101 ccatctgata tctttaaccc ttgaccgcca atcttagtat ctaatttttc cggcaaacgt
    20161 tctacaaatt catacatata agaggctttt agggcatcat tgatttctgc gtcacttgct
    20221 tccggattcc caaaacgaag attttctcgc aatgtactgt caaatagcca agtatcttgt
    20281 aacaccattc caaaactttt acgtaatgta tcacgttgga tagagcggat gtcatggtca
    20341 tcgactgtaa ttgttccttt tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg
    20401 gattttccgg cccctgtttt tcctactata gcaatggttt gacctggata agcagtaaaa
    20461 ttgaaatctt cgatcaaacg ctgcttgggg tcataagaaa aagaaacatg atcaaaagag
    20521 acttttcctt tgatattttt gggttccaag gcataggcat ctggcaaatc aggcttttct
    20581 tctggctgat cgatcaaatc aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt
    20641 gtcgtgattc cagaaagttc gataaatggt ttagaaaact gacttgagta gatcgtaaag
    20701 ctggaaatca caccaacagt gattgacgaa ccactcttca atgccaatag gccgccaact
    20761 aaaccaatcg ataaataagc aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa
    20821 gaagaaaatt gtgcccgttg tcctttttca tatagatcag catttagcag ttcaaattgc
    20881 tgttgactag cttcttcttg ctgaaaagct ttcacgattt tctggttacc gattcgttcg
    20941 gtcacaaagc ccgagatatc cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa
    21001 gcacgagcaa cgatccaact cacaaggaaa ataattggtg tagaacaaag aacgaccaat
    21061 gtcagaagtg gacttagacg catcataaaa ataaacgaaa gaatgatgat tgccactcca
    21121 gaaaataatt gattgaaggc cgcagaaatt gcaatggaaa tattgtccat atcatttgta
    21181 aatcgactga cgatatttcc gtgggagttt tgatcataat aacgaagtgg caatcgattc
    21241 agatgagcaa atgcatcttt tcttaatttt gcaacggata agtaagctaa ccgattgctt
    21301 aacacttgga tgagccattg actgactacg ttgattaaaa cgataccacc gaatagaata
    21361 agtaaatgga acagtcgatt gaagcggacc tgtccttctc caatcatttg atcgatactt
    21421 tgaccaattt gtaacgtcat cacaactgtt gcaatcccat tgatgattcc tagtatcaca
    21481 gctagccaaa tgtcttttgc ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc
    21541 aaggataatt tttttacttt cataactatt cttcctcctg tgatgcgacg atttcctgat
    21601 attctgctga ttgagcaagt aattcttcat gggtaccctg agcagcaatc ttaccttgat
    21661 ccatcactaa gatatgatgt gcttcttgaa tagagcggac acgttgggag ataataatca
    21721 ctgtactgcc aagataagtc tgtagcgcct tacgcaaatc caagtctgtt tgataatcca
    21781 acgcacttaa agagtcatct aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa
    21841 tcgtcagtcg ctgtttttgt ccgccagaga aatttttccc attttccatg acttttgtat
    21901 ctaaaccatt tggaagctga cggacaaatt cttctgcctg agcgattttc agagcttccc
    21961 aacattcttt ttctgtggcg tctttcttac cccattgaag gttttcacga atagtcccgg
    22021 taaatagtac agaagtttgg ggaacaagag caatttgctg acggagatga tccagctgcc
    22081 aagaacgaac atccattccg tccattataa ctgtaccgga actgacatca tagaaacgcg
    22141 gaatcaattg tgtcaaagtc gttttcccgc ttccggttgg accaatgatt ccaaggactg
    22201 tatttcgctt cagctgaaat gtaatatttt ggagagaaag accagaatca ggagtataac
    22261 ggaagtcaac atggtcaaaa gcgataattg cttcagaaga aaaatcggga gtagtactga
    22321 cctcagcttc aagaatcgaa ttttctgtgt ctagcacctc tttcacccga tttcctgaag
    22381 cttcggcacg tgtgaagatc acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt
    22441 gattcatata attgatcaaa gcaagaactt gtccttgttg caagctacca atatttactt
    22501 tgatcccgcc aactgtcagc aaacagatga tcccgacatt catgatcaag gttgtagcag
    22561 gtgataatag ggcagagata ttggaaacac gtaaataatt gttcgctaga tcatttgtgc
    22621 ttttatcgaa cttctctgtt tctttttttg tctgcgcaaa tgcgcggatg acacgaacac
    22681 cgctaagatt ctgactaacg tgttcattga gctgatctag tttttcttgc actttttggt
    22741 agagaggaac ggtttttttg atgataaaaa ataaaatgat tgagaaaata ggcaataatg
    22801 ctaagaaaaa cagacctact tcccagtcga tcacaaaagc catgaccact gacccgatac
    22861 ttaaaaacgg tgctctgatc aataaacgaa tgaccattgc taaagctagc tgaagctgat
    22921 tgatgtcgtt tgtcattcgg gtgatcaagg tatctgttcc aaaatgattc aactcgttgt
    22981 gagagaaagt attgattttt ttgattagct gattacgtaa ctctgtacca aatccttgtg
    23041 aagctactga agcatagtat tggcaaatga tagcacaaat cagaccaatc acagacatag
    23101 cgaccatcca taagcccatt tcaatgattt ttcctcggtc attcattttt agtccattat
    23161 cgatcaagga agccatcatt aaaggcagga caagttcgaa gacagcttca agaaatttga
    23221 agaacgggcc caaaatgatt tgcttacgat agtcttttgc ataacgtaat aactgaatca
    23281 tatgattcct cctattattt atttgtccac aattgaaaaa acaagtacta ccacattgta
    23341 gcttaggaaa tttagaatga aaagagaaca agccttttca taaatgaaag ttttattatg
    23401 aagaagcttt agaaattgaa tagactattt tttttaaaaa aaagaaaatg agaaaacatg
    23461 ccagtctctt cattcatagg aacgtctatt ttacttaatt tttctataaa tgaattctgc
    23521 ataaaaaata tatcaagtaa atgtttatag atgaaactct atttacttct tttttaattt
    23581 gttcttgcaa tcttttttgc tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa
    23641 ttgattttca aagaaggagt atgaaaatga gaaaaatatt tattcgttta ttgtgggtac
    23701 tatcttagag gaataaataa tagtggagaa aactatggtt tggcgcagat tctatcaggt
    23761 ttgatggaac gaagacaacc tcccgacatc aagtcaaaag ccacttgaga ttttgatctg
    23821 aaaataaaaa aagaaaacaa ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa
    23881 tgggaaaaaa ctaagagtgg tgtacgtaat caataaaaag gatggtgaca acagtacctc
    23941 tttactctct gaaagcatca taactcgttt ttcctcatta tgccaataaa ttgttgttat
    24001 accacatata aggatttgta atagctaaaa acagacagga aagttgaaat ctgtgaatct
    24061 tattttgtat ggaaggattt atagaacgca aaaatcaaga taaagatgaa taaaaatcct
    24121 gtttcggcta tatattgcgt atctacgcca ataaaacgag tttgttctgt catagccata
    24181 taaagcaaag gcaaaaatac aactacagcg agataagatc cgatgatttc tcgaaaagtt
    24241 ttccaggaag tatgtttcct gtttttatat ttatccgatt tcattgaata gtcatagcct
    24301 ccttattctt tcatatgttg aaattccagt ataaaaactg gaaggaactg tacgtttttt
    24361 agaataaaaa ttataaggga taagtaggct gctgtctata ataagatata tttttcaatt
    24421 ctttattaaa gattttattg acaaaatgaa tccgttttct atgatggagg aggggtgaat
    24481 aagttgttga agaaggaaat tttatgttac ttagagaacc agacagcctt tttcgattta
    24541 gaacatatga acgaggtctt tacagcacaa aggttagctg atcattttgg ggtaaaaaga
    24601 aacacagtca gtcattattt gaaccagtta aatgatgagc aactactagt caaaatcaat
    24661 tctcgaccag tgatttattt ccacaaagaa gcttttgaaa aacaattttt taagttacgc
    24721 acgaattttt atttttctat cgatgatctc aaagaggaac agcctttttt cgcgcaacca
    24781 aaagacttat tctcttttat gatcggatac gacgccagtt tgaaagaaag tatcgaacaa
    24841 atcaaaacag ctctttacta tccagatggc ggattaccac ttttgattac aggcgaaagt
    24901 ggcacaggta aaagttattt agttaatttg gtctatcagt attgtctgct tcatgatctg
    24961 ctagaagatt ctgcaccatt catcactttt aattgtgcac aatatgcaga taatcctgaa
    25021 cttttgacta gcaatttgtt tggacatgtt aagggagcgt acactggtgc agaagaaaat
    25081 aaaaaagggg cttttgaagc agcagatggt gggatcttgt ttttagacga ggtacatcgc
    25141 ttaacaccag aaggtcagga aaaactgttc acctatcttg atcaaggtgt gatttatcgt
    25201 atgggagaac ctaatttgtc acgccggatc aaaacacgtc tcttttttgc tacgactgaa
    25261 gagattacta gtcatttctt aacaactttc attcgaagaa tacctatccg aatcgaacta
    25321 cctgcactta atcagcgtag tcgtaatgaa cggctagaac tgatttactc tttttttctc
    25381 gaagaacaaa gaaaaatggg gagccctttg tcagtcagtg gaccagcttt gtcactttta
    25441 acaagtcaga aattatccgg aaacatagga gagctgaaaa atatcgtcaa agtttcagca
    25501 gcaaaagcat atgctgaaca gcgtgaacag cctgaaatcc atgtcacgat ccatcatttg
    25561 caaaaagaac tacttgcgca gcctattact aaaaatacag cacagcaagg aatctatatt
    25621 acgcaagacc agaccttaga gcaattaatg gagaagcaac agcccgaaca gcagcgaatc
    25681 gttaaaagtt ttgagcaaat cctaattgat ttcaagaaaa ataactgtct gctatattct
    25741 tgcgaaggaa aattaaagca agaagtgatt caattatttg attttttgct gtttgagacc
    25801 agtagacaag aaaaacacga gctgctggtt tattggacac aatctatccg agagaccttt
    25861 cgacaaatgg aaactgccta tcaaatcaaa ttcgatggaa acagtgtcta tgccttgagt
    25921 tactatctct atcagcgaag cactatcaaa tggttaccgg aagaacatga tattatccaa
    25981 ctaatcaaag aattagaaca acaagtttct cagtcttatc catctagtta tcactatgtc
    26041 caacgaattc tggaattaag taagatcaaa ttagatattg aagacacaag tatggatcgg
    26101 attttactga cgatctatct aaaaaaagcg aaatggtcaa aagaaaaacg actgccaaaa
    26161 gcgatgattg ctgctcacgg ttatgcaact gctagcagta tggcaaatgt agtaaatcgt
    26221 ttattacaag aggatctatt tgaatcattt gatatgccac tagatgtcac accacagcag
    26281 attgcttcag aaatccttga ttattgcgaa aacagcgatg tttcaaatgg attgatcata
    26341 ttagtagaca tgggatctct gaaagaaatc catcagtttt ttaaaaaaca gttatccgta
    26401 ccgcttttga ttttgaataa tgtgacgaca cctttagcaa tcacagtagg ggaatgcctg
    26461 caaaaaaatg tatcattaga agagatagca gaagagactg ttcgacagat ccagccagaa
    26521 tggcgactgc tctatccaga ggaaaacaaa cccaaagcac tcattactac ttgcttcact
    26581 ggaatcggta cggcggtcca cttaagtgaa ctgctagaaa aaagcttacc tacggcttgt
    26641 cagttgaaaa tcatcccata tgaatatcag caattaaaag ataagaaaaa cagtgaccca
    26701 ttattttcca tatacgaagt cgtaggaatg attggcacca cagatcctga tatcaccaat
    26761 atcgactatc tttcattaga agacctcatc tcgggggaaa agatgtctgt actagctgaa
    26821 tggttggaca ctactatgaa tgcttcagag aaggaaatat tcaatcagcg catcattcgt
    26881 aatttttcat tggaaaaagt actggattcc gtgactattc tagatacaga gaaggtgatg
    26941 ggagaaatcg atcattttat gagagaatta gaggtgcagt tgaatcgaag catcagcaat
    27001 cccaaaaaat tagcgttgta tgtccatgtg agctgtctca ttgaacgatt gatccgacaa
    27061 atgccaatcg aaacctatca aggattagag aaatggaagc agtgtcaaaa agaagggtta
    27121 tctgctatta aatctgcgtt tagtgtcatt gaagagcatt atagtgtcat gatccccaat
    27181 tctgagattg cctatatcta tgatattttg agtgaagata cagagtttct ctcgatagaa
    27241 gaagaatttt gaacataagc aagttgttgc cactaatttt ggcatgcaac ttgctttttt
    27301 agtatgtgta agtgaaaaga ggagatgaaa aagtggatag agaaattatc ttagcttccc
    27361 atggaaaatt tgcttcaggg atattagatt cattagagct gatctatggg aaaaatcatt
    27421 ctattactgt attcgattgc tatactgatg agaattttga tttagcggaa acagtaaggc
    27481 agctttttat gaggtacaaa gataaagaaa tcattgttct gacggatctt tttggcggaa
    27541 gtgtcaacaa cgaattctta caatatatca atcgggatca tgtctatctc gttgccggat
    27601 tgaatttgcc actattgatt gagctagtat ctcggttgga cgctgaagta gagacaactg
    27661 ttttgatcca gcaggtgcta actgattcaa aaaaaatgat ccagttttgt aacgagcggc
    27721 tcaatcagaa aatagaagaa gacgaatttt aggaggaaga aaaatgattg tattgacaag
    27781 agtcgaccat cggttattac acggacaagt agctttttca tggacacaaa caataggcgc
    27841 agactgtatt ttgattgcta atgatgatgt acctacgaat gagatacgaa agacaacgat
    27901 caaattagcc aagccacaag gagtcaaact agtgatcaaa tcaattgatg actcgattgc
    27961 cgcgttgaaa agcggggtga cagataaata taagttattc atcgtagtgg aatcgatcga
    28021 ggatgcatat aagctggcag aagcgtatcc agaaatcaaa accatcaact taggagggat
    28081 caaggcaaaa cctggtactc gcagcatcgg aaaagcggta aatattttac cagaagagga
    28141 ggaacgcttg aaacgattaa ttgacaaaca aatagaagta gaaatccgtc aagtaccagc
    28201 agataaaaaa gtggatgtaa gagaagtttt gtaagaaagg aagaacctca atgattgtac
    28261 aagcagtctt attaggaatt gttgcatttg tcgcacaatc agaatatgca ctagggacat
    28321 cgttgctttc tcgtccgatt gtgacggggc tgtttacagg aatcgtgtta ggtgatgtaa
    28381 aaactgggat cattatgggc gcaactttag agttagcatt tatcggatcg ttctcagtag
    28441 gtgcttcgat tccacctgat gttgtgaccg gtgggatttt agggacggct tttgctatta
    28501 cagctggagc cggtacagaa accgcgcttt tactaggttt gccgattgct acgttgacat
    28561 tgatattgaa aaatatttac ttagggcttt tgattccaat tatgaaccat aaagcagaca
    28621 attatgcaga agaaggaaat tataaaggag tcgagcatat gcacttgctt gccggtatcg
    28681 gtctttcctt gatgcttggt gttgtggtaa tggtttctta tatggtggga agcaatacga
    28741 tcggaaatct attaaatatg attcctgact tcgttcaaaa ggggttgtca gtagctacag
    28801 ggttgattcc agctcttggt tttgcgatgc ttgctcgttt gctgatcaac aaacaagttg
    28861 caccatattt tttcttaggt tttgctatag cttcttactt agagattcct gtgaccggag
    28921 tagctatttt tggtgcgatt ttagcagttg tggtagtcaa tattatgaat gtccgtcaac
    28981 taccacttca aacaactgca ggggaggtag aagaagatga agaattctga gatcaaaaac
    29041 agcaaaataa cgaaaaaaga gctaaatcac gtattttggc gttcttttca aatggagttt
    29101 tcttggaatt atgaacgtca gatgaatatg gcttatgttt atgcaatgat tccgattttg
    29161 aaaaagttat atcagtcgaa agaagaaatg gcagcagctt tgaaacggca tctagagttt
    29221 tttaacacga ccccacatat cgttacactg attttaggaa tcacggctgc aatggaggaa
    29281 gaaaataagg atgatcctga atttgatgtg actgcgatcg atagtatcaa aacatctttg
    29341 atgggacctc ttgcagggtt aggagattct ttcttttggg gaactttgcg attgattgcc
    29401 acaggtgtgg gtacctcttt agccatgcaa ggaaatattt taggccctat cttgttcatt
    29461 ttgattttca atattccgca cattctgttt cgctatctag caacaggttg gggatatcgc
    29521 ttaggaacgg gttttttgaa aaagatccag gcaaacggta tgatggaaag tttgacatta
    29581 ggcgcgtcta ttattggact gatggtcgtt ggagcaatga ctgccacgat gatcaccatc
    29641 aatattcctt taaaaatcgg ttcaggagaa aatgctgtga ctgttcaaag tatttttgat
    29701 gatatcgttc caaatatctt atgtcttgga gcatttggca tagtgtttta tctattgaag
    29761 aaagaagtga aaccattaac gatcttgatc ggactagctt tctttggaat ttttggttcg
    29821 tggataggga ttttttaaaa aaggagcaga gaaaatgttt acgatgcaag attatatcta
    29881 tgaagaaaag gaagtactaa gtacgatctt gaaaaagaat gatttttcga caagagaaca
    29941 tatgaaaaaa acagtgaatt tactgatctt agcaacgggt tcatcttata acgcttgttt
    30001 agcagcgaag ccagcattgg aatcttatgg tgatcttact gtcgatattc aagaaccttt
    30061 ttattttttc aattatggaa aactttctcc ttcaatcgat actgtgattg ctgtgtcaca
    30121 aagcgggaaa agtgcttcta ctgttgaagc agtaaaaatg atacaaaaac aagggttacc
    30181 tgttgttgct atcacaaatg acgtgcaaag tccacttgct ttagaagctg cacaaattat
    30241 tgatttaggt gccggggtag aatcagtagg cttcgttaca aaaggctatt ctgcgactgt
    30301 tttacagtta ctactcttag ggataggaat tggaataagt aaaaataaaa tcagcaaaaa
    30361 aatagaacaa gactacatgc aacagttgcg caaaataatc aaccatttac cagcgattat
    30421 tcaaaaaacg gaagaatttt ttgatggata tcaaagttta tttcgtttag cacagcgttt
    30481 cgtcaccatt ggatatggtc caaactgggg aacagctaaa gaagcagaga cgaaattgac
    30541 tgaaacaatc cgcgttccgt cacaaggatt tgagttagaa gcttatatgc acggcccata
    30601 cctggaggca gatgcttcgc atttgctatt ctttattgaa ggggaaagtg taaataagga
    30661 acgttcgcaa aagttgcaaa gatatatgag tcggtacgtt ggagagactc tcaccattac
    30721 cacaaaaaaa gcaagaaacg agaaaacact tggattagca atagaatgtg atgaatatct
    30781 gtctgtatta gcgcttgtcg ttccgtttca actattcgct tataaaaccg cagtagctaa
    30841 aggtattgac ttgaacaaaa agatttttga agactttgat acagtcttga aaagtaaatt
    30901 ataagaggtg gaaatcatgc tgaaattcaa tgaagaagaa caaatcaaag caataaaagg
    30961 cgcactagct ttacgtccgc aagtagaaga aatcatcgat aaactttatg cggaaaaatt
    31021 tgacgctgtc tattatctag gaatcggtgg aacgtatgct tcctcgatgc aagctgtcac
    31081 ttatatgaat gggaaaagca acttgcctgt ctttgtacag catgctgcag aatattacac
    31141 tactggaaac aaaagattaa caaaagattc tatcgttatt ttgtcctctg ttaccggtac
    31201 aactcaagaa gtagtgaaag cagtagaaca aatcaagaaa gtaggagcga cactgattgg
    31261 atttatcgat aaagcgaaca gtaagttgtc tcagctatgc gattttgtgg tcacttatcc
    31321 agctcctgga acagaacaaa tcaaattttt tatggcagca gatcgattga tgtatttgaa
    31381 tggagaattc gaagcttatt cagaatacta tgaacagctc gaacgttatt taccaacagg
    31441 gttagtggaa gcagagaaaa aagcagatgc atttggctta tcatttgcag aaaaacatcg
    31501 acgtgatagt atgcattatt ttattggagc agggaatcaa tggggagctg tttattcata
    31561 tgccatgtgt tactgggaag aacaaagctg gcttccatcg aaatctatcc atgctgccga
    31621 gtttcttcac gggacgttag agatcgtaga agaaacaacc ccagtcacat tatttttagg
    31681 cgaagatgaa cagagagtgt tatctgaacg tgtggctaaa cttttgccaa aaatttgcag
    31741 caattataca ttgatagaca caaaagacta tccagtagaa ggtatcagtg aaaaatatcg
    31801 cggacgtgtt ttgtctttct tattgatgca tgtagtgaca caacgcatcg atgctcatgt
    31861 ggagcagttg aactgccatc cattagagat acgccgctac tatcgtcagt ttgattatta
    31921 ggatagactg gataaaaaga atataaaaaa taaagagagc agcgataact gctcccttta
    31981 tttttttaaa atattggcat caattacgtt ataaaacaca ttttttgcat tatctacagt
    32041 tcaacttata atctgtttaa tgggacatgt gggtgccaac cattttttgt tatgcaatag
    32101 tactatttag ttgttgcatg gatccgtagt aaaagtccaa gtacaacgag ttgcgccagt
    32161 ttacatagcg ttttgtatcc agaatgttga ctaaaacaaa agttcctatg tcaaccgtca
    32221 acagagacaa tctgtccgtt gcaggacctg acagatttag aaatgttcaa tctgtctctt
    32281 atgtatttta aatcaatact cactaggaga agtttcctaa cctcctaata gtaatgctgg
    32341 tagtaacatt gtcaaaactg cttcctctta ggaacaagca aagcataacg agtgtaaaaa
    32401 taaaatgtag actattgtaa catatcattg attatttcaa gtaaaaacga taaaagaagt
    32461 aaacaaacac ttaaaaacaa tggaaattag tcccaacttt aagaaatgta tactataatg
    32521 gaaattatca attatttatt tggaggaata tccatgaaac aaaaaatcgc agttcttggt
    32581 cctggatctt ggggcactgc tttagcacaa acattggcag aaaacgggca tgatgtccgt
    32641 atttggggaa atgtacctga acagattgat gaaatcaata agtatcatac aaatcagcat
    32701 tttttacctg atttgacgat cccagaatcg attatcggat ataaagaatt ggcagaagca
    32761 gtggataacg cagatgctat tttatttgtt gttccaacaa aagcgattcg ttcagtcgca
    32821 caggaactta ttggtaaaat gaatactaag ccaatcatta tacatgcaag taaaggacta
    32881 gaacaagata cgcataaacg aatctcagaa gttttggctg aagaaattcc agaagaaaaa
    32941 agacaggcaa ttgttgtatt atctggtcct agccatgctg aggaagtggc agtacatgat
    33001 atcacaacaa ttacagctgc aagtattgat caaaaagcag cagcctatgt acaaaaactg
    33061 ttcatgaatg attatttcag gatttataca aataatgatg tgattggcgt agaaactggt
    33121 gcagcactga aaaatatcat tgcaattggt gccggcgcta tccatggatt aggattcgga
    33181 gatgatgcaa aagcagcaat catgactcga ggattggcag aaatcagccg tcttggtgtc
    33241 gcgatgggtg ccaatcctct gacatttatc ggtttgagtg gtgtggggga cttgatcgtt
    33301 acttgtacaa gtgttcattc tcgcaattgg cgtgccggta acttgttagg acagggacat
    33361 aagctggaag aagttctaga aaacatggga atggtcgttg agggtgtggc aacgacaaaa
    33421 gcagcagtag agctagctca acagctaggt gttgagatgc caattactca aacaatctat
    33481 aatgttttgt ataatggaga agatatcaaa aaagcagcaa aagaaatcat gttacgtgac
    33541 ggaaaaatgg agaatgaatt ttctcttcgt taaatagatg atcaatagtt aatcgtcgtt
    33601 taggaggaac agcaagtgaa ggtcaaaaaa gcagtcattc ctgccgctgg gctaggaaca
    33661 cgttttttgc cggcaacaaa agccatggca aaagaaatgc ttccaatcgt ggataaacca
    33721 acgatccagt ttattgtgga agaagcattg gattcgggaa tcgaagacat cttgatcgta
    33781 acgggaaaag aaaaaagacc cattgaagat cactttgatg caaatatcga attggaaaac
    33841 aatttgcggg aaaaggaaaa aacagaatta ctcgctttag ttgaagaaac gactcaagtc
    33901 aatcttcact tcatccgtca atctcatcca agaggactgg gggatgccgt gcttcaagca
    33961 aaagcattta taggaaatga accgtttgtc gtcatgctgg gggatgattt gatgaaagat
    34021 gatatcccat tgactcaaca attgatgatg gattatgaag aaacacaagc ttccgctgtt
    34081 gccgtcatgc gtgtaccaga aaatgaaaca tctaaatacg gaattattga tcctaaaaca
    34141 gatagcgaaa agggtcgttg cagagtaaaa gggtttgtag aaaaacctga aatgggaaaa
    34201 gcaccaagta atttagccat tatcggacgt tatctactga ctcctaaaat atttgagata
    34261 ttagaaactc aagaaccagg agcaggaaac gagatacagc taacggatgc acttcagact
    34321 cttaaccaga cagaggcggt ctatgcgaga gagtttaaag gaaaaagata cgacgtaggc
    34381 gataagttag gatatatgaa gacaaatatt gaatatggac ttcaacattc ggaaattggg
    34441 acttcattaa gtgcatatat tatccagttg agtaaaaagc taagaacaga aagttaactg
    34501 gaaataagtg gcctgctgag aatgaaaggc tatttgttat taaagcggca gcttgtgcgt
    34561 catttgcgca actgtcgttt tagttttttg actctagatc agttgagtcg tggaaaacga
    34621 aagatagaaa aagactttcg tgaatagatt cagaaaatat tatgctaatg gtttaatttt
    34681 tttttaagtt gtataaaaga aaatgattac ataagagaaa tgaaagccaa gccagaagat
    34741 tcatagtaga ataaaaaggc gaatttttta attataaaaa aaggtgtgtg ttcactatgg
    34801 gaaaattagt cgctgttgca cagtcgctgc ttgatatact caagtggctg atggaaggaa
    34861 gcaattggat atttgtgttg ccactattgc ttattagtct atttatcatg tttcgcatgg
    34921 tcagaaaaaa agaaacgttt ctgattttta tctggtatgc agcatccgtt ggggttgcta
    34981 ccttgatggt tgaattggcg ttaggtagtt gggaagcttg cgtatttgct ttattttttg
    35041 ctgtactgta tctaatcatt tatatcccat atatgaaaag aaagtctcgg ataggcagaa
    35101 aaggtagaca tacaactact ttttctagta cagattctct cgaacaaagt agaagaacga
    35161 gaagaagacg gtaatattta gtaggtatac gcaaaaaaag ataaaataat ggataaacta
    35221 ttgagatgag agatttattt aattggtgga aaaaagcttt tttcacttat ataaaagaat
    35281 cttttttaaa ggaatgcttg ttgtatcggg ataagccatt ccttttttta tgttatttat
    35341 atatatataa atcataccaa tgtttttttg tattttcttg cttgcacttg gataagagat
    35401 tacttacaat aaattcagat agaaacagct gaataaaaaa aggctccctg acccttagtt
    35461 ggcgctaaga atcagggaat gaccagtcac acacatacac cctgaccgat cattttgcca
    35521 tagtcaatgc agtgcatcga cctaatttat tgtacctaat ctagggtgaa aattctaccc
    35581 ctatgtagat attttccatg gtctgcacgt tgttttattt gctatgtcaa ggtattgggc
    35641 gtattcgttc aatgcctttt ttgtgtttga aacagcaaag accacatgtg ttactgtcta
    35701 actactgttt ttttatgtac ttccttagat taggcagctg tttctattca tgtcaagtat
    35761 agctaggcgg gagagataaa tgagtttatg ttattttgac tattatcacc aattggaaga
    35821 gaaattaagg atagaagagc cgctgatgct tcaagctggc acttggatcg cgaaacaaat
    35881 taatcaaggt ggtagattgc agatatatgc tagccgcaca ctaagtggtg tagcatttga
    35941 attttgggat caaaccccgg atcttgtacc aagtatattg attgaaaatc cagccggagg
    36001 tgtctatgaa acgctagaag gtgcaggaca ggcaattatt gatcagcttt ctgtcaaatc
    36061 agaagacatt tttcttcttc tttcgaatga aggaagaaac ccggcaatca tcgaactagc
    36121 tcagtggatc aaagaaaatg gtcatccatt aatcattgtt actggatttg atttgtcacg
    36181 aagtatcaaa tcgcaacata gcagtggttt gcgattattt gaatttgctg atttggtttt
    36241 ggacaaccac gccaatttac atgatgcagt cttgactttg ccaaatctag atccagcgat
    36301 ctgcggaaca gcctctcttg caacgatctt tttgctgcag caagttttgt attttacagc
    36361 aaaacagcta gtccaggaca caagtggaaa atagttatcc acatgttgac aaagttcctt
    36421 aaaaaaataa tgttccacat aaaaatggag cgtttgaaaa aaagaaaacg attattaatg
    36481 aggattaaca acgactactt tttttgtttt agttatttta agatggatca tctttgattt
    36541 taagcttgtt ttgtttaaag tttgttattt ttataagttg tttcacagtt atgagaaatg
    36601 agttataaga atatgtagtg tcagaaaaat aaaaagtagt ggaaaaagag acaagaaaga
    36661 atccatatgg attctttctt gtctcttaat acacgttgat gaaagagtct tccccaaaac
    36721 tctttacaaa gtaattatag tacatttgtt atcattattg caatgaagtt tacctgatat
    36781 aacaaaattg tccaacagga tacctttttc taatagaaat aaaatacaca aaaattgaag
    36841 gatagataat ggatatttat aagtgtttat tatttttttt gcaatgaaat tcaatttttc
    36901 aatgattgta aaatgaaaaa aacaggacga gattttctct aatctaaaaa taaaaaaaga
    36961 attttaagga aaaacagaga aataaaatac ttaatataaa tggaaagaga aaaaaataat
    37021 catacatagg agaaaaaaaa caaaatagaa aaaagtgatt ttctaataaa ttattcatgt
    37081 ggaaatgtca tttttactgt ataattcatt tgattatcca ttttttattc atcaaaatta
    37141 ctgaaaaaag aaaggaagaa ataaatttga agaaaacaaa ttcttcagga aaaccgaaac
    37201 gcgacttcac taaattatcg acaccccata cgtatgtcat tatttttggt gtagtcatct
    37261 ttgcatggat actgactttt gtcgttccag caggaaagtt cagcacgcaa gatattgaat
    37321 acaaagatgc aaatggcgaa acaagtactc gtactgtttt acgtcaagat tccttccgtt
    37381 atgcttacga gctggataag tcgtatgtgt ttgatcaatt agaagaactg caagatcatc
    37441 cagcagaacg tgaaaaactg gatgttccgg aaaaaggatt ggaaaaagtc attgctgacg
    37501 gagaaaaaaa tcttacgcag gaaaaactag atgaaatctc cttgacagat gatgtgcttt
    37561 acgatcagta cggcgaaaat atctatgata cttctaaaaa attgcataaa acagccaaaa
    37621 tatggggaac agatgatttt ggcggctttg gtttcttaaa ttttgttttt gaaggactag
    37681 tatctggaga taagtatgga tctgctgtag ggatcgctgc attgatcctt gttgttggag
    37741 gtgcttttgg tattattatg cgtaccggag cgatcgacgc aggtatctat gcgtttatca
    37801 gtaaaactaa gggactggag cgtttagctt taccgctatt attctttgct ttttcattcg
    37861 gtggcgctac attcgggatg gcagaagagg taattccatt ctcaatggtg atggttccat
    37921 ttgtcattgc gttaggatat gactcgatcg tagcagtaac cgtgacttat gtcgcatctc
    37981 aagtcggaaa tgcgacctct tggatgagcc cgtttagcgt agcggtggct caagggattg
    38041 ctggaatccc cgtgttgtct ggtgcgacat tccgattgat tatgtgggta gttgttactg
    38101 cattagccgc tggttatatg atgatatatg cagaaaaaat ccgtaaaaaa ccagaaagtt
    38161 cgttgactta caaatcagat gattatttcc gttctcatat caaaaagaca tctgatgaaa
    38221 ataaaaaatt catgcttggg cataaattga tcttattaga gatgttagtt gtccttgtct
    38281 ggatcgtttg gggcgtgact caaaaaggct attacattcc agaaatcgct tctcaattct
    38341 ttgtgatggg attagttgct ggaatcattg ctgttctttt caaattagat ggaatgaaag
    38401 tcaatgacat cgcttcttct ttccaatctg gtgcagctga tttagctggt acagcaatcg
    38461 tagtaggtat ggccaaaggg attcttcttg tcttgggagg atctgatgca tctgttcctt
    38521 cagctttgaa tacgattttg catgggatcg ggacggcgtt gaccggtgtt ccagctgtca
    38581 ttggtgcttg ggcgatgtac atcttccaaa gtttgttcaa cttggttgtt acatcaaatt
    38641 caggacaagc agctttgact atgccaatca tggctccact ggcagattta gtaggtgtat
    38701 ccagacaagt agcagttctt gcttatcagc ttggttcagg ttttatggac gcttttacac
    38761 ccgtatcagc cagcttgatt ggtgtgctag gtgttgcacg gatcgaatgg gcaaaatggg
    38821 caagattcca aatcaagatg caaggattcc tgtttgtttt aggaactatt ttcatcatga
    38881 ttgccattgc aataggtctg cagtagtact ggagacctgt aaataaaaaa gtaatccaaa
    38941 cagtagataa ggactccaag aaccgtcatt ttacggtaaa atcaaaggag taaagatcct
    39001 atcgtttgga ttatttttta agatcctatc atcagttata cttaaagaaa gaggaaaaat
    39061 gtatgaaaat cattcgacaa attgaagtgt ttgcaccaga ttacttagga aaaatggatg
    39121 tactgattgc cggtgggaag attttggctg tagaagaaca gctagaaggc ggctacgaag
    39181 gagtggaagt agaagaactt tcaggtgagg gaaaagtact aaccccgggt ttcattgact
    39241 gtcatttcca tattttaggt ggaggaggag aaggcggtta tcaaaatcga acgccagaag
    39301 tcacattgag ccaattgact acagctggtg taacgacagt tgtcggttgt ttgggaacag
    39361 acggagaagg tagagatatg actgcgttga tcagcaaagc gaaaggtctg gaagcagaag
    39421 gaatctccac ttatgtatac gaaggttcgt accgattgcc ggtaaaaaca gtaacagatt
    39481 ctatcatcaa agactttttg acaattgata aaattatcgg aatcggagaa atcgcagtat
    39541 ccgatcaccg ttcttctcag ccaagttttg aagagttcgc tcgtgcagta gcagatgctc
    39601 gtgtaggagg catgttatcc ggaaaagctg ggatcatcaa tgtccactta ggcggaggaa
    39661 aaagaaagct agagctattg acacgaatcg tagaagaaac agaaattccg atcacacaat
    39721 ttttgcctac tcatgcaaat cgcacaccag aattatttga agcttgcgtg gcatttgcca
    39781 aacgtggtgg cacgattgac ttcactgcta gcgaagatcc tgatttttgg gagaagacag
    39841 atggtgaagt tcgcttcagc aaagcactga aacgattgat cgaggaagat gtttctttag
    39901 ataatttcac gatgacctca gatggacaag gaagcttgcc ttattttgat gaaaacaatc
    39961 atttcctagg gttaggagta ggaagtgcca aggcattgct agtagggatc aaagaagctg
    40021 tgcaaaaaga aagtatccct ttggaaatcg ccttacgagc tataaccagt aatccagcac
    40081 gtatattgaa attggataaa aaaggaaaaa tcgaaattgg tgctgatgca gatctgtgta
    40141 tcttggataa agaaacgctt gatattgata cagttatcgc taaaggagaa ataatggtcc
    40201 aagaaaaaga agtcaaagta tgggggactt tcgaaaaaag tttttaatca acagctctaa
    40261 ctatcttttt tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat gttaccctga
    40321 aggtagatca ataaacaaac taagaggagg aagcacaatg gcagatttaa aaggacgttc
    40381 taaagatatg aaagataaag tagtcggtga aggaaaagaa gcatacggca aagcaacaga
    40441 cgacaagagc aaagaaacag aagggaaagc acaatcacta ggtgctgaca tcaaagaaaa
    40501 agctcgaaat ttaggcgacg aagtcaaaga caagttttct cgaaaagacg aaaaaaaata
    40561 aaaaccactt attactaatt gaatcaagca aataaaaaat ccgaaccata tctgcgagac
    40621 agatatggtt cggatttttt atttgatcat tatcctgttt tttaaacttc gattttttga
    40681 aattttttat tttttagttc aaagacttca tcacagcttt ttgctacttc aatctcatga
    40741 gtcacgatga taatacagcg attttgttca tgggcgattt cttggaaaag tttgacgata
    40801 tcttgtgaag ttgtttcatc tagatttcca gtaggctcat ccgctacgat tagttgatgt
    40861 tcacagcaga tagcacgaac gatagcgact cgctgctgct gacctcccga aagtttggtc
    40921 acctgttttt ttgctaatat ttcatcaatt cctacacgag ccagtcgatc taaagcaact
    40981 tgtcgataat tctcttgctt ggtcttagtg atttccattg ctgctatgac gttgtctaga
    41041 gccgataaat aaggaagcaa gttgtaagct tggaaaataa tcgagacatc atttttacga
    41101 taacttctta acccgatttt gttcagtgaa cgatcttgat agtatacctt tccttctttt
    41161 ggtgcatcta gtccagaaat aagcgaaaga aaagtcgttt ttccagaacc acttgctcct
    41221 aaaatagcgt acatttttcc agcttcgaaa ttcagttcta catctttata aagcgcatct
    41281 tgcggatctt gataccaaaa tcccaattgt tctgtccgta acatcaaatt cctcctttag
    41341 tttaatagga tttttcttgg gtttaatctt aatatcccta tagaagctaa aaagacagaa
    41401 aacagactga ttccaaatgc tactccggct aatagtccca attgtgcagg tttgacattg
    41461 atttccaatt cgctgacttg atcagaaaca gcaaatggat tattcgtggt tccttgttga
    41521 ccatcgcgtt gaccgccttg ttcttgtctg ttacctggca tttgtccacc gccaggagct
    41581 tgctggtttt gtgtcgtggt ttctgtctgt tggtcaagta gctgctgtcc cacggcattc
    41641 gcgacgacgt ttccgctaag cgaagcaact cctaatgcaa gaatcataca aatagccact
    41701 tcggtaaaca attggagaat gattttactt ctggattctc ctaaagatag caagacgcct
    41761 aactcatgcc gacgctcccg aatggtaatc ataatgatca gagtaagtat gataataccg
    41821 gctacagcca ctagtaaaac aacattttta gaaaaacttg ccacgttgtt taaaggttcc
    41881 aacattgact gatacatgga atcatttgat tgtaagctaa atgtatctgt gtcgatcagc
    41941 ttctcagctt ctttgagaaa ctcatccatt ttatctggat cagatagagt gtaaatagca
    42001 gaatctatcg tgttatcgtc agatgtccca tttagttcat ttgcaaaagt gtaagaagtg
    42061 taaagtgtat tcgacggatt catgaagttg aatttcatac ccatactgcc gcttgtttcg
    42121 ctggattcat aaattccttt gatcgtcatt tcataagtgg tatcttcatc ttcagtactc
    42181 gtgattgtga aagtatctcc gactgccagg tcatttgttt ctgctagagt agaatcaatg
    42241 actacattgt ttgtgccttt gtcgctgtca tctattcctt caccatccgt gatttttgct
    42301 gttccatcag aaaaattact attttgtgca gttgctgaca caccactgat ttgaaagtct
    42361 gcttgagtca tttgcggatt cccgcccatt cctccagcag gttcaccgcc tgcatcagta
    42421 gtttctgaac tgtcttcact tgaatcagaa ctagtgatgg cagttattcc atcttttgcc
    42481 atagctgagg cggaactttc aaaggaataa ctctttacat tatctaattc agctattttt
    42541 tccgcgtccg ttaaggaaac tggtgtaaga gaaaaactgc ctggatctgg acgttcttca
    42601 gtggtagaac cgcttgaaga agtttgttcc tgttttttaa atgaagcttc tcgattggca
    42661 cttaaagtca ctgttgctcc aacttctttt tgtgcttgat tcgctgcttc ttctgcagcg
    42721 cttcgaatcg tgaggccagc tagcacaaaa ataagaatgg ctgtgaacac ggcaaacaat
    42781 aacaatgaac gtccccattt tacttttgta cttttccatg cacgtttcat aaaattcata
    42841 ttttgcatcc ctcctagtag atgcatttga taatagacaa cctatcttaa agctagctta
    42901 aatcttatgc tgtttgcaca ccatcaacat gaggttctgt atatataaaa agtcactata
    42961 gttattgcaa aaaatgtggt aaaatgaaaa aagtatgttt caaaccgacg aagaagaaat
    43021 gaaagaggaa tagcaaaaga aatcctagat gctacagagg gatttttacg tgcatagttg
    43081 ttctaacaat tgctgatact agcgatttag tttatttgtt gtataataat ggggaacagc
    43141 tgaatactta tataagtatt ctggctatag cgtttcgcaa aagatccatt ttgttaatga
    43201 agtatatcca aaataaaatg cccatccaac tgtcagaacg cggatgtgct ggtaattttc
    43261 ctgctgattc tccataagtt gagaaatagt gattttgcat atgcttcatt ggaatggctc
    43321 atatatttag ataaagaaga aaatcgacat ctttaaactt ggatgtcata agaagaggtg
    43381 aagtttgtga gtaccattaa aattatccca ttaggcggcg ttcgtgaaaa tggaaaaaac
    43441 atgtatgtcg cagaagtggg cgaggatatt tttattttag attgtgggct aaaatatcct
    43501 gaaaatgagt tgttggggat cgacgtcgta atccctgatt ttacgtattt agaagaaaac
    43561 attgatcgtg tagctggtgt ctttttaaca catggacatg cagatgcgat cggggcactt
    43621 ccttacttgt tgtcaaaaat ttctgtacca gtattcggga cagagttgac gatcgagtta
    43681 gcaaaattga atgtcagtcg taatgaagca gccaaaggat ttaaggattt ccatgtggta
    43741 gatgagcata cagaaataga ctttggtgaa acagtagtga gctttttccg taccactcat
    43801 acgataccag attcagtggg gatcaatctg aaaacaaaag aaggtagtat cgtttatacg
    43861 ggagatttta aatttgacca aacggctatc ccaatgtatc aaacagatta tgcacgattg
    43921 gcggaaatcg gcaaagaagg agtcttagcc ttactaagtg aatcggcaaa tgcagaaaat
    43981 cctcagcctg tagcttctga agcccaaata gcagatgaag tatttgatac gatcaaatat
    44041 tgggaagggc gtatcatcgt cgcttgtgta gcaagtaatt tgcaacgagt gcaacaagta
    44101 ttggatgcag cagaaaaagc acaacgaaaa gttgtcttga ctggtcaaga tttcgaacga
    44161 attattcgca cagcgatgcg tttagaaaaa cttcaattac caagtgaaga tctgcttgtt
    44221 acgttgaaag acatgaaaaa ctatgatcca gaagaactga tcattttaga aactggccga
    44281 atgggtgaac ccatcaagtc tctgcaaaaa atggcaaacg gtacacaccg taatgtcaag
    44341 attgaagaag gagatttggt ttatatcact actacgccaa gcatcgcgat ggaaaccatc
    44401 gtggcgaaaa cggaagatat catttatcga gctggtggaa cagtgaagtt gatctctgag
    44461 aatatgcgcg tttctggcca tgccaatcct aatgacttgc aattaatgat caatttgatg
    44521 aaaccaacat attttgtgcc agttcaagga gagtatcgtg aattagctgc tcatgcagat
    44581 ttggctcatg cagtagggat gccatacaaa aatatctata ttactggacg cggagacgtg
    44641 ctggaatata agaacaagcg tatgagtgtt gcaggaacta caagtgcaga aaatgtaatg
    44701 attgatggat taggcatagg tgatatcgga aacattgttt tacgtgatcg aaaaatcttg
    44761 tcagaagacg ggattttcgt agcggttgtc actatcagtc ggcgagaaaa gaaaatcatt
    44821 tcaaaacctc aaattacttc acggggattc gtttacgtaa aagctagccg tgatctgatg
    44881 aaagaaagca gcaatatgat tgaaacaatc gttgagaaac atttagaaag caatgatttt
    44941 gagtggagca aattaaagca agacatccga gatcaattag gccgttactt atttgaacaa
    45001 acaaaacgcc gcccagtgat cttgccagtc attatggaag ctactcaacg tcgaaataaa
    45061 aaataaacaa aaagagtaag atgaacctct ctatagagtg ctcgtcttgc tctttttttg
    45121 cgaaaaacaa ataatttttg aatacagcat ccctcgcttt ataataaacg atagaataaa
    45181 gggagtgaat caatgatgag tcaaacaaaa gtcgaacgtt atttagaaga acaaaagatc
    45241 agctatcaac cacttgattt tagtcatgtg gtagaagaat ctttcacaga agaactacaa
    45301 aaaagaggga ttgatccagc tttgatatgt aaaactctcg tagtgaaagg aaacaaaact
    45361 ggggtgatca tcgcattgat tcctttgaac gatcacttag attacaaaaa aacgagacaa
    45421 gtgacgaaag atcgtaaagt tggttttcct ggaatggatt ttgtattaat gcatacagga
    45481 tatccccacg gagcaaacac ccctattgga ataaaactcg cacatcctga ttattgtttt
    45541 ttagcagaca gttctttaaa agaaaaagaa gaagtaattg tgtcaagcgg tgaaatcggg
    45601 agaagccttc ggataaaagt agccgattta gaaaagatcg tccatcctat ttacgctgat
    45661 ttaattagtt aagggacgag aagagaagct aagcagagca gagacctcaa acttgagatt
    45721 atatctgctt ggtacttggt gcatcctgta ttatctgtta caattgcttt gtacatactg
    45781 tactatcaat ccaatgaaaa tatatttagc agctatattt tttcattgat gtgcaagtga
    45841 tgaaatcgtt tttaataaaa aagatacaga gaaaacgcga ggtgtaaaat atgaaaatta
    45901 cgtatcatgg acattcctgt ttaacaattg aaatggaaaa tggccaacgc gtgatgattg
    45961 acccgtttat tacaggaaat ccattgacag acttaactgc agaagaagta aagacagatt
    46021 ggttgctgat tactcatggt catagtgacc acataggaga tatgattcct attgcaaaag
    46081 aaaatcaagc cacaattatc agtattgttg agattgctga ttatgcgaac agcagaggag
    46141 tagattcatt cggtatgaat atcggtggaa aacatgcgtt tccattcggc gtggtaaaat
    46201 tcgtacatgc acagcatagt tcaagctatg aagtagacgg tatcgttcag tatatgggcg
    46261 agcctagcgg aattattatt caagcagaag gaaaaacgat ttatcatgca ggggatactg
    46321 cttatttcag tgatctagga ttgcttgcag aagaattcga tattgatgtc gctttcttgc
    46381 cgattggcga taactatacg atggggccag aagatgcagc aaaggctgcg caaacaattc
    46441 gtgccaagaa agtagttcca atccattaca atactttccc tgtcattgaa caagatcccg
    46501 aatcttttgt agagttactg gaagatagtg aaggaaaaat aatgagcgtc tcggaaacgc
    46561 tggaagtgta ggcagaaaat ggcgacaaaa catgatttga ttttagaata tattgaaagt
    46621 ttacctgttg gcaatcgaat ttctgtaaga agcatcgcaa aagaattgaa tgtcagtgaa
    46681 ggaacagcgt accgtgcaat caaagatgca gaaaatgtcg gactagtttc aacaattcaa
    46741 cgagtaggga ccatccgtat cgaacgcaaa ttaaaaaaac atattgaacg tttgacattt
    46801 ggcgaggtcg tcaggatcat tgaaggggac gttttaggcg gggcggcagg attagacaag
    46861 gtcttgaata aattcgtcat cggcgcgatg actaaacatg ccatggaacg ctatattacg
    46921 ccaggatcac taatgatcgt cggcaaccgg gcggaagtcc aaaaattagc attggaagat
    46981 ggcgccgcag tattgatcac aggaggattc aatacgactg ctgagattgc aaagctagct
    47041 gatgatcttg aaatgccgat cttacgcacc tcttatgata catttacagt cgcaaccatg
    47101 atcaaccgag cgttaagcga ccaattgatc aaaaaagata tcatgcttgt cagtgatatc
    47161 tatatgccag ctgaaaagac tcattatttg cagcagacag atacgatttc tgattatcag
    47221 cgtttgtcag aagaaacgca gcattcgcgt tttccagtcg tcaaccgcca tcatcgtttg
    47281 atggggattg ttacggcaaa agatgtttta gggaaatcgc caaaccagat catcgatcgg
    47341 gtgatgacga aagaaccaat cagtgtaaag aaaacaatga gtattgcctc tgttagccat
    47401 caaatgatct gggatgggct agaagtcatg ccggtcgttt ccgatgactt gacattggaa
    47461 ggcttgatta cccggcaaga tgtaatgaaa gcgatgcagc ttgtccagcg acaaccgcag
    47521 attgcagata cgatttcgga tcagatatct ggaaatatcc atacgattga tactgaccgt
    47581 gaaggaaatc gtttggaaaa ccctaagttc aaattcgtag tagcaccaca aatggtcaat
    47641 ggcgttggaa ccatttcttt tggcgtactt agcgaaatca tttctgatgt cgcacaaaaa
    47701 acaatggtga tgaaccaaaa acgaaatatc ttgattgaac aagtgaacct acattatttg
    47761 cggctgatcc aattggaaag cgaattagac attcgtccac gtgtattgga aatcggcaga
    47821 cgttccgcta aactggatat cgaagtgttt attgaaaaca ccatcgtttc caaagcaatc
    47881 gttgtttgtc aagtaatgga acgttcttag aaagcaggag tgaaatgaca gtacagcaag
    47941 aaatagtaga tacaataaaa aaatacgata aaatcattat ccacaggcat ttgcgccctg
    48001 atcctgatgc attaggatca caagtaggat tagccgaact attacgggaa agtttcccag
    48061 aaaaagaaat acgtcaagta ggtgaaacga ttgaagggct tcgcttttta gcagaaatgc
    48121 aagaagttga agatgtcttc taccaaggag cgctagtcat cgtgactgat acagctaatt
    48181 cacccagaat cagtgatcaa agatatcagt tggcagaaaa actgatcaaa atcgaccatc
    48241 atcccaatga tgacccttat ggagatcttg tgtgggtaaa tacaaaagca agtagctgta
    48301 gtgagatgat tgcagaattt gctttgatgt ttcctgaatc tttaaagatg aacgttaagg
    48361 ctgcacggtt actttatgca ggaatcgtcg gagatacagg gcgctttttg tatccctcca
    48421 cgacgtcccg tacattagag attgcttcga tattgagaaa ttatccattt gatgcaagtg
    48481 ctttgaacag agagattgaa caaatgccga tgaaagtcgc taaactttcc ggttatcttt
    48541 atcaaaatat ccaggtggat gaaaatggtg caggaagaat aatccttccg caagaaattc
    48601 ttgataaata tggaatcgat gattcagaga cagcttcgat cgtttcactt cctggagtga
    48661 ttgacgaagt gctggcttgg gggatttttg ttcagcagcc ttcagggtat taccgcgtac
    48721 gtttacgttc taaggggccg atcatcaatg aattggctaa acgccatc
//
LOCUS       DAYLZO010000007        47455 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.007, whole genome shotgun sequence.
ACCESSION   DAYLZO010000007 DAYLZO010000000
VERSION     DAYLZO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.007"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..692)
                     /locus_tag="ACGVJG_000358"
     CDS             complement(<1..692)
                     /locus_tag="ACGVJG_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFJ6556346.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKT"
     gene            992..1864
                     /locus_tag="ACGVJG_000359"
     CDS             992..1864
                     /locus_tag="ACGVJG_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6556347.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(2322..4520)
                     /locus_tag="ACGVJG_000360"
     CDS             complement(2322..4520)
                     /locus_tag="ACGVJG_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556348.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            4752..5744
                     /locus_tag="ACGVJG_000361"
     CDS             4752..5744
                     /locus_tag="ACGVJG_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6556349.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            5804..7225
                     /locus_tag="ACGVJG_000362"
     CDS             5804..7225
                     /locus_tag="ACGVJG_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFJ6556350.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            7218..8717
                     /locus_tag="ACGVJG_000363"
     CDS             7218..8717
                     /locus_tag="ACGVJG_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFJ6556351.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            8756..10150
                     /gene="uxaC"
                     /locus_tag="ACGVJG_000364"
     CDS             8756..10150
                     /gene="uxaC"
                     /locus_tag="ACGVJG_000364"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFJ6556352.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            10309..10992
                     /locus_tag="ACGVJG_000365"
     CDS             10309..10992
                     /locus_tag="ACGVJG_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFJ6556353.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            10994..11830
                     /locus_tag="ACGVJG_000366"
     CDS             10994..11830
                     /locus_tag="ACGVJG_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFJ6556354.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            11841..13595
                     /locus_tag="ACGVJG_000367"
     CDS             11841..13595
                     /locus_tag="ACGVJG_000367"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556355.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(13871..14212)
                     /locus_tag="ACGVJG_000368"
     CDS             complement(13871..14212)
                     /locus_tag="ACGVJG_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFJ6556356.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(14280..16451)
                     /locus_tag="ACGVJG_000369"
     CDS             complement(14280..16451)
                     /locus_tag="ACGVJG_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFJ6556357.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            16662..17519
                     /locus_tag="ACGVJG_000370"
     CDS             16662..17519
                     /locus_tag="ACGVJG_000370"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFJ6556358.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(17590..18447)
                     /locus_tag="ACGVJG_000371"
     CDS             complement(17590..18447)
                     /locus_tag="ACGVJG_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFJ6556359.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(18432..21134)
                     /locus_tag="ACGVJG_000372"
     CDS             complement(18432..21134)
                     /locus_tag="ACGVJG_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFJ6556360.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(21147..22436)
                     /locus_tag="ACGVJG_000373"
     CDS             complement(21147..22436)
                     /locus_tag="ACGVJG_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFJ6556361.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            22586..23632
                     /locus_tag="ACGVJG_000374"
     CDS             22586..23632
                     /locus_tag="ACGVJG_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6556362.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            23634..25787
                     /locus_tag="ACGVJG_000375"
     CDS             23634..25787
                     /locus_tag="ACGVJG_000375"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFJ6556363.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(26260..27711)
                     /locus_tag="ACGVJG_000376"
     CDS             complement(26260..27711)
                     /locus_tag="ACGVJG_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFJ6556364.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(27708..29432)
                     /locus_tag="ACGVJG_000377"
     CDS             complement(27708..29432)
                     /locus_tag="ACGVJG_000377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFJ6556365.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(29425..30039)
                     /locus_tag="ACGVJG_000378"
     CDS             complement(29425..30039)
                     /locus_tag="ACGVJG_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFJ6556366.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(30384..31841)
                     /locus_tag="ACGVJG_000379"
     CDS             complement(30384..31841)
                     /locus_tag="ACGVJG_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6556367.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(31882..32802)
                     /locus_tag="ACGVJG_000380"
     CDS             complement(31882..32802)
                     /locus_tag="ACGVJG_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6556368.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(32814..33761)
                     /locus_tag="ACGVJG_000381"
     CDS             complement(32814..33761)
                     /locus_tag="ACGVJG_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6556369.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(34240..34959)
                     /locus_tag="ACGVJG_000382"
     CDS             complement(34240..34959)
                     /locus_tag="ACGVJG_000382"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFJ6556370.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(35008..36654)
                     /locus_tag="ACGVJG_000383"
     CDS             complement(35008..36654)
                     /locus_tag="ACGVJG_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFJ6556371.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            36833..37423
                     /locus_tag="ACGVJG_000384"
     CDS             36833..37423
                     /locus_tag="ACGVJG_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFJ6556372.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            37515..39020
                     /locus_tag="ACGVJG_000385"
     CDS             37515..39020
                     /locus_tag="ACGVJG_000385"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFJ6556373.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(39104..40456)
                     /gene="celB"
                     /locus_tag="ACGVJG_000386"
     CDS             complement(39104..40456)
                     /gene="celB"
                     /locus_tag="ACGVJG_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFJ6556374.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(40456..40974)
                     /locus_tag="ACGVJG_000387"
     CDS             complement(40456..40974)
                     /locus_tag="ACGVJG_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556375.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(40990..41316)
                     /locus_tag="ACGVJG_000388"
     CDS             complement(40990..41316)
                     /locus_tag="ACGVJG_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFJ6556376.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(41329..43308)
                     /locus_tag="ACGVJG_000389"
     CDS             complement(41329..43308)
                     /locus_tag="ACGVJG_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFJ6556377.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(43329..43643)
                     /locus_tag="ACGVJG_000390"
     CDS             complement(43329..43643)
                     /locus_tag="ACGVJG_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFJ6556378.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            43811..44104
                     /locus_tag="ACGVJG_000391"
     CDS             43811..44104
                     /locus_tag="ACGVJG_000391"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFJ6556379.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(44182..45294)
                     /locus_tag="ACGVJG_000392"
     CDS             complement(44182..45294)
                     /locus_tag="ACGVJG_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFJ6556380.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            45447..46295
                     /locus_tag="ACGVJG_000393"
                     /pseudo
     CDS             45447..46295
                     /locus_tag="ACGVJG_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(46648..>47455)
                     /locus_tag="ACGVJG_000394"
     CDS             complement(46648..>47455)
                     /locus_tag="ACGVJG_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFJ6556381.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        14226 a         9566 c         8450 g        15213 t
ORIGIN      
        1 catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa ctgtgtatcg
       61 actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt ccagatcact
      121 tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat tttttcaaat
      181 cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa tacataagta
      241 atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt ttgaatcccg
      301 tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat tggcatcgga
      361 aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa tgtaaagaat
      421 gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact tcccattatt
      481 cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg aagataccta
      541 aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt attcgttttg
      601 attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga tagcgtcagt
      661 tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt gtgaatgttt
      721 gctgcttatg acttcatgat agataactat ctgaattacg tatataatca tttttaccgt
      781 aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa agtgtatttt
      841 ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca cttagcatag
      901 gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga aacagaaaca
      961 tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta taggagaaag
     1021 aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct gtatcgttcc
     1081 ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg cgaaaaagtt
     1141 tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt ctacgccgac
     1201 catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga tcgtcagtat
     1261 gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg gtcgaggttt
     1321 cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc caacgttctt
     1381 agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc ctgtggctgt
     1441 taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg atagtttgga
     1501 agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa ttatgctgcc
     1561 attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt actggaacac
     1621 ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac agattctttt
     1681 acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg ttgatgtgat
     1741 tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc ctatgctgat
     1801 tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt ctgtcaaagg
     1861 gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct tcaagaaata
     1921 agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt atttttcgaa
     1981 gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca gccttgagcg
     2041 atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata agaagcgatt
     2101 ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag cctgtctctt
     2161 gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt tctttttgcg
     2221 tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt ctaacttctt
     2281 ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata tttttttcga
     2341 tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt ttgcggattt
     2401 cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt caaccatttt
     2461 tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc tgtttccttt
     2521 ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat cacatctaaa
     2581 gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga caaattttta
     2641 aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa ttgttctaaa
     2701 acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata aatttgtttc
     2761 atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac gagttcattg
     2821 atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc aaacaatgca
     2881 tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat ggcttcaaac
     2941 aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc agcaggaacg
     3001 atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata cagggcttct
     3061 tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt actgatgcct
     3121 atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg gcaatactcc
     3181 atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt ggcttgcatt
     3241 tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc cgtgatcatg
     3301 ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc taacagatcg
     3361 atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa ttgctgcaac
     3421 cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac aaatagcgtt
     3481 tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc tcctatgatc
     3541 cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt tgatttaaga
     3601 ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa taagtaggaa
     3661 agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat tctcagattg
     3721 cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca attattataa
     3781 tctgatttca gtactatata cgttttatct ttttctctta tcactttcag ctgattgtaa
     3841 tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt ctgagtagta
     3901 aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc accaacaact
     3961 tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc tttttcaaac
     4021 attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa taaattattt
     4081 ttgttattta tgtacttctg ttgatacttt tcaacttcct cttcggtcag ttgactcaac
     4141 gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt attatcatcc
     4201 ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt ggagtaatct
     4261 gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc atacgtagaa
     4321 aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg cctgagcaaa
     4381 ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg gtaaatgaat
     4441 aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa tattttataa
     4501 aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag gctttcattt
     4561 ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa gaatagcata
     4621 attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat caaagatcta
     4681 ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga aaaagtaagg
     4741 aaaatgatgg aatggtaacg atcaaagata tcgctaaagc agctggcgtt tctcatacga
     4801 cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa caaaaaatac
     4861 gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt ttagtgaatc
     4921 aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact tcatcaaatt
     4981 tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac agtttatctg
     5041 ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac gatggactga
     5101 tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa gaaaaagaat
     5161 ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac gtcactgccg
     5221 atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga catacgaaga
     5281 tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa aaaggggtat
     5341 tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta acaggagatt
     5401 acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct gacctgccta
     5461 ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct gtacatttag
     5521 ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct ccagtcgtct
     5581 cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg tgtcaagaag
     5641 gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct aaaattgagc
     5701 tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca agtaatttta
     5761 tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa aactgacaaa
     5821 agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg aaggcaattt
     5881 tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat tgtttaaagg
     5941 taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga accagcaaga
     6001 taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg atacttccga
     6061 gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg cttatctatc
     6121 tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg caggaatcca
     6181 attcgtggag actgataatt taacagatac tcccccacag agcttcccgg gcaaactgac
     6241 agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca ttccttgcga
     6301 actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg cagaaaaatg
     6361 ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct gttgtagttt
     6421 agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca atcaacggca
     6481 taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg tgatcgaagg
     6541 gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg tcatcatcac
     6601 agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac cgcatacagc
     6661 gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa tgaaagatcc
     6721 tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta tgctttcttt
     6781 acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct tgaatccttt
     6841 tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca gcacccgtct
     6901 gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt taatcactgt
     6961 ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac atgatgatga
     7021 aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta ttcctcgatt
     7081 attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg tgacagatac
     7141 agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat tacaagaaat
     7201 gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta aatcgacgag
     7261 acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg attgatcagc
     7321 aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg ggtgatttga
     7381 aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg acagacgtaa
     7441 ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt gaattggatt
     7501 atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga acatttcaag
     7561 gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta gtacctaccg
     7621 ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa aaacatccag
     7681 atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt tcgcagcttg
     7741 gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat cccaatgccg
     7801 gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa ttcaaaaaaa
     7861 ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat gtttacgatg
     7921 agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa aatgaccagc
     7981 gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt tctgatggtc
     8041 tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg attgctcaag
     8101 gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa gtattgatgg
     8161 cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat gacttcaaac
     8221 agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt aataaagcag
     8281 gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca ggaagctctc
     8341 ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt agtttattgc
     8401 aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat tgccagttgg
     8461 tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg gtcaaggtat
     8521 ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat gcaggcgtct
     8581 tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc atcgctgtag
     8641 ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc atttttaaaa
     8701 acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa gatttatgtt
     8761 tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc attcctacgc
     8821 aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa tttacgaaaa
     8881 caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca aatggcgctt
     8941 gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg attttgaaaa
     9001 atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt acagttggtc
     9061 acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag aaaatgcaga
     9121 gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc gtcgggaaat
     9181 cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag acgacttgca
     9241 ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt cattccgtcc
     9301 agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac tactggaaga
     9361 agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag ggcagcggat
     9421 agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca tcttacgcta
     9481 taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag ctaacaaact
     9541 attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt tgacccactt
     9601 ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta actgtaatac
     9661 acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg atctttcttt
     9721 gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt tgccaaagac
     9781 gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga tgggctgttt
     9841 ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca acgatactcg
     9901 agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg ggaattttgt
     9961 cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat atttccgtcg
    10021 tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg aagatgaaca
    10081 acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt actttggctt
    10141 ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt gaaataacgg
    10201 aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt cttcttctct
    10261 tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat gcctactatc
    10321 catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc cgagacaggt
    10381 tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa ccaagcaaag
    10441 aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct agcttcacag
    10501 tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa aatccaagaa
    10561 gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt ttttgaatgc
    10621 gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg agattactta
    10681 gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat gcgtgcattt
    10741 gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga attgttccag
    10801 accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga tacttgtaaa
    10861 aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc tgataaagtt
    10921 gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa ttatttacaa
    10981 aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt tagatgttcg
    11041 aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata tccaatttgc
    11101 accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg ggagtgctgc
    11161 ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag gatgctatat
    11221 caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt tcaaacagca
    11281 aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg gtagtgtctc
    11341 tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca gaaattctat
    11401 cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg aagcagggat
    11461 caatcatcca atctataact atcagttagc taaacgttta atcgatgaag tagcttcacc
    11521 aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc atgctgatca
    11581 ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag cgcttcatct
    11641 caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca agggctgttt
    11701 agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt acgccactct
    11761 tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt ttatttacca
    11821 agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa aaatatcatg
    11881 caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt tttgccatgt
    11941 caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc gctattgcca
    12001 atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt tatttcaacg
    12061 cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt catgtaaaat
    12121 accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt atcaagaaaa
    12181 tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc cagtcaaaga
    12241 tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc agcggattaa
    12301 atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt cctgttgttt
    12361 tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt ttccgtaaaa
    12421 ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct gcaaaagtga
    12481 tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa cacgaaacaa
    12541 ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta gatatcctgc
    12601 aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg atttgtttga
    12661 ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc gtgatgtatc
    12721 agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg attcctacga
    12781 tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct ggcgatgtag
    12841 aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt tttgaggatg
    12901 tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct ctagaggtga
    12961 aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct acactcctta
    13021 acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat gatcagcctt
    13081 tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct caaaccactt
    13141 tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac gtatcaaaag
    13201 aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac cagcttcctg
    13261 atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc caaaagcaac
    13321 gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta gatgaagcaa
    13381 cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat tacctgacag
    13441 aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag gcagataaaa
    13501 tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa ctgttagctc
    13561 aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct atgacaagac
    13621 ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    13681 gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg taactcagag
    13741 ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc tatcggcaat
    13801 aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc tgttgtttca
    13861 taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata cggttcaagt
    13921 cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt acttcttctg
    13981 attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg aactcttgtc
    14041 cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg aacagtttgt
    14101 gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg aattctggta
    14161 attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac atgttatgcc
    14221 ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt tcaaaggggc
    14281 tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc ctgaacgcct
    14341 tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc atattgattc
    14401 aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc aacttgatct
    14461 gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc tgtttttgaa
    14521 gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac atttccactt
    14581 gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt cgaaatcact
    14641 acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc gaaattagtt
    14701 tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc agccgctgtt
    14761 ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc ctttgatatg
    14821 acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga atcaacgatt
    14881 ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact gtaagcaccc
    14941 gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc ataatactta
    15001 tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt ttgtaaaccg
    15061 atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc ttgatacatc
    15121 ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact ttgcggttcg
    15181 tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata tacgcttaat
    15241 ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa tcctctaaag
    15301 acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg atctaaagct
    15361 actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa tgcactgttt
    15421 tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata gattttatag
    15481 cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc atcgatcact
    15541 gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt gtcattaaga
    15601 tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat ttttccgttt
    15661 tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg gtgatcgatc
    15721 ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc agacagcgta
    15781 acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc ccataccccg
    15841 ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct gtacttcttt
    15901 tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg gtctaatgtc
    15961 agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc tgatgttccg
    16021 ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa cccgtgatgc
    16081 tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg agaaatcgcg
    16141 tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc gtttttctta
    16201 tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt tgcttgtttt
    16261 gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat cagcaaaatc
    16321 aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa ttgtccgaga
    16381 tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc taaaatgctt
    16441 tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt ttaccatagt
    16501 gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac gttagtgcaa
    16561 gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat gttttaaaaa
    16621 tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc acgatcactt
    16681 tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa tggttagttc
    16741 ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat caacaacctg
    16801 ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc gaagaaacag
    16861 attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt ttatatgaag
    16921 atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca aatgaaccga
    16981 cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa cagcaacgac
    17041 cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt gcaaaaaatc
    17101 catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat cggagatatc
    17161 aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa attggacgag
    17221 atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc gccttgacac
    17281 atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt ttggaaaccg
    17341 gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc gttggagacc
    17401 ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa ctgcacatga
    17461 tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta tgggaataga
    17521 aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct ttttgtgtaa
    17581 tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga taatcccact
    17641 ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt ccatataacc
    17701 acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct gattcattac
    17761 cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga tcagtgcttg
    17821 agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat cttgcccttt
    17881 gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac aatctttatg
    17941 ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa tatgtcccat
    18001 ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg caccgattcc
    18061 tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt gcttcaattc
    18121 cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt agcgttcctt
    18181 gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca aaacgtatcc
    18241 gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc cgattccctt
    18301 caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg gtgttgtggt
    18361 agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca caaattttgt
    18421 tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat tgtccgaatc
    18481 tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag cagattcaat
    18541 atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact agacatattg
    18601 aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt aattgcaaac
    18661 gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc atgatgtttg
    18721 atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt agagaatttt
    18781 tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag acactgtgct
    18841 tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag tagcgtcact
    18901 gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc gactgttaca
    18961 ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt cgtggggttt
    19021 tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact atctgcttcg
    19081 tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc tttcatctga
    19141 ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt catgattgtt
    19201 tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg aaattccaca
    19261 accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc catttcctgt
    19321 agaatactta tttttgctat ttccggtgta tctactaata cttttacttc agagttttct
    19381 ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata ctcatttcct
    19441 atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt tttgcgatct
    19501 tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt ttcgatgatt
    19561 cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc ttctacaagc
    19621 gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac tttgatcatt
    19681 cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa ttggacagtt
    19741 tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg atacttacgt
    19801 ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc gaatcttttt
    19861 ctttctaatt cgatggttat ttctgcctca ccagttttat ccattcctgc agtatttaca
    19921 atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc ggtcagctgt
    19981 tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa acgaggtatc
    20041 atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg atgattttgc
    20101 attaatgtct tccaagcata atctaattga tcatggggat agtttccact aacttcataa
    20161 gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt ggtattccat
    20221 tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc atccgtttct
    20281 tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt ttgaactgct
    20341 tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag ttcatttgcg
    20401 agatggattg cttttgatat atctttttgt accggttgat gatcaacacc attcatcatc
    20461 aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt ccaaaaggca
    20521 agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt ggcaaatagc
    20581 agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta ttgggaagaa
    20641 tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc acggccaaac
    20701 gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat atttccaaat
    20761 gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc ttccatgcca
    20821 atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg caagatataa
    20881 aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg tcttttttct
    20941 ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg aaaactgttg
    21001 aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat cagtcgcatg
    21061 tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga aatgatatat
    21121 actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa gtaatccatg
    21181 actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata attttcagga
    21241 ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc tacaagtaaa
    21301 ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat agctaatgca
    21361 atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc gtactttcct
    21421 tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag ataatcttcc
    21481 tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac attactgtca
    21541 tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt ttgccctttt
    21601 tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt caatttctgt
    21661 gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc cacataacct
    21721 aacacgacca ccgcaaacat attggatggt accagatatc cataacggca agtatcatcg
    21781 ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc tcgcccgtca
    21841 ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg agagtgttca
    21901 tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat aaaagattga
    21961 ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg tattgggtag
    22021 cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt tgtatgatct
    22081 gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg atcgatattg
    22141 atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc ctcatcttct
    22201 tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca catcgctgga
    22261 atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt tgtcaacaga
    22321 gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc cccacatttt
    22381 tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata agccactaat
    22441 aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc caattatgtc
    22501 tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat tatttgaata
    22561 tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa tatttaatga
    22621 tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac caactgaaaa
    22681 agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat tgactgaatt
    22741 agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta aatccgcttc
    22801 tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc ttcttccata
    22861 tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga tgcaagataa
    22921 acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag caaaagagct
    22981 aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac atcttgactt
    23041 actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa aaatctatga
    23101 gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg ctgcctctta
    23161 tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga ctcatccaca
    23221 atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg gtttgacttt
    23281 ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt atatcactaa
    23341 gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga ccatgaatct
    23401 cttgagacag catggttatc atatccctga agatttttca attatcggat ttgatgatat
    23461 ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt ttgaacagtt
    23521 aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc gaccatccga
    23581 tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat aaaatgaata
    23641 tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct aatgggaact
    23701 gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa actacggatc
    23761 aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac cgtcttaccc
    23821 atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg atcaatggaa
    23881 aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa gaaagtatat
    23941 tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct acattgatac
    24001 ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc ggtttacttt
    24061 tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg gaaggaaaaa
    24121 tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata ttgagatttg
    24181 aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac tcgatcccgt
    24241 tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt atcagctcgg
    24301 aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact taccttgctc
    24361 acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac catgatcaac
    24421 aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca caaacatttt
    24481 atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga tctataaaac
    24541 ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt tatcctcttt
    24601 attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg aacagctata
    24661 gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt atgcctggga
    24721 cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga gacttgatgc
    24781 ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat ccaaattatg
    24841 gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac taccacgaat
    24901 cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa gttgccaagg
    24961 tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac tatcaaaata
    25021 tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag ttcagagaac
    25081 cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg caaagcagct
    25141 ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt gagagatata
    25201 aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga ggatatggtt
    25261 ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta gctatttcaa
    25321 accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct gaaatggcac
    25381 agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg ggttaccctg
    25441 gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg ggattttatc
    25501 ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac aaggctgttt
    25561 tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg ccgcagcaac
    25621 aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat tttacccatc
    25681 aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct caccctaaga
    25741 aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa gaggttgtga
    25801 aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc tcatattttt
    25861 cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat tgagaggttg
    25921 tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt tcctcatatt
    25981 ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta gtggagaggt
    26041 cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca
    26101 tttttgcata aatttggctt aataccgaga aactggcttt tgaactccgt ttatcgagag
    26161 gtcatgaaaa agctgccttg cctcaagtaa taagaacagc ccaaaattag atttctagac
    26221 aattttttat aagttatttc attacattgg ctttttcttt tatgattctc cactgttttc
    26281 tcggtactct ttaggggatc tgccgacgat tttcttgaat aatttagaga aatacgtggt
    26341 gttgttgtag ccaatctcac ttgctatctc attgatgttt tgattactgt gcaaaagcag
    26401 ttgtttcgct ttttccatac gtaaatggtt caaataagct gaaaaacttt ttttcgtttc
    26461 ttttttgaac agctgtccta gatacatgac atttaaatgc aggttttctg acacttcttt
    26521 gagtgtaagt ggttcttgat aatgctggtg caggattacg attacttttt ccacattgtc
    26581 actgtacttt ttctgtctct gatgcttttt gatgaatgac agaagtagct gctgaagttc
    26641 ttgcaccgtt tttgcctgat ggattttttc gattccttgt aaatattcct catcttccag
    26701 atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa ctggaatatc gtatatcttc
    26761 cggcatcatg gctgctaact gaaactgttc aaaaaaatca gtgatcattt tttgagcgac
    26821 ttctaattgt ttattttgca gcacgcgtct gaaacttgaa aaatcgatgg tttgatcttt
    26881 tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta tctccataaa attgatgcaa
    26941 ctgtagattg tctttcgctt gttggaatgt ttcagggatt ttgtccaaag aatctgtctc
    27001 ttccccgata ctgatcagcc attcctgatc aggaaagttt tcttgaacaa aacaacagaa
    27061 gccatcaact tcatctgcct ccaacaacaa cgtgatcaat acacggtctc cgtaatttcg
    27121 ttgatagtaa aagatttgtt gccgttcttg caaccatcta ttgataagag tatcatgagt
    27181 tcttgccagt tgaatcaata ctatcctatg ctgcttctca cttatttgtc ctattatttc
    27241 ttcttcactt atttcatcca attcttcatt caaccattga ctgaataaca gttcttgata
    27301 gatttcttgt tgattttttt gttcattttg ctgatctaac cgtgttttga ttttcttgag
    27361 gctttcaacc aattcgaact tattcacggg tttcatcaaa taattcacgg cgcctaattg
    27421 catacctcct ttcaaataat cgaattcttg gtatcctgat aaaatcataa attcaaaatt
    27481 atgacgttct ttttgagccg cttcaataaa ttccagtcca tttatttctg gcattgtcac
    27541 atccgttact actaaatcaa tctgtcgctg ttcaagtaca gctagtcctt ctttggcatt
    27601 ctctgctgtt gctactattt gaaagcctaa actttgccaa tcaattatct tttttaaccc
    27661 attcaaaatc atgtattcat catctaccaa taacacttga tacacgctca tccctccttg
    27721 aatgtaatag aaacggtcag tccacctgtc tcattattct caagcgacat ataataactg
    27781 ctgccgaagc tggcacgtaa acgttcattc acgttttgca gaccgataga accgtgaagt
    27841 tccactttag gatgagacag tttttcttca atctgtttta gccgtttttc agtcatccct
    27901 tttccattat ctttgatgat gatccttacc cgtttgcctt cctgcaagac tttgacactt
    27961 agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa aataattttc aactaatggc
    28021 tggatcacaa atttaggtac ttcgatcttt tccaaatcag gatcgatcat aaaatgatag
    28081 gctactcgat taggataacg catttgatac aaatagacat atttttcgca aaagtccagt
    28141 tcttctttta aagtgatcgt tttctcttga ttcgtattgt ttcgaagcaa agcggaaaac
    28201 gcatacacaa catctgctaa ttcttcactt ccttcgctga ttgcatacat tcggatatat
    28261 tctaatgtat tatataaaaa gtgtggattg atctgtgctt gaagcgcccg catatgtgcg
    28321 tcttgctgtt tgatttctaa tttgtagatg tcttctacaa actgattgat gctatccaac
    28381 atttcgttga taccgattga aagttccttc agttcatatt ccgtgtttgt tatgtcgatc
    28441 cgcgtctcta aattccctgc tgctgtttga tccatggcct gcatgacgat agatacttgc
    28501 tgtgaatatc gtttgaatgt tttataaaga aagataagca gtatattgtc taaaataatg
    28561 ataccaatga tcagcggggc aagatttttt cccgtttcga tttttacact ttttttatct
    28621 agtacagcta atatacgata atcatcagac agttcttggt gttgaacaaa attgtttttt
    28681 tccagatcgt caatcggcaa ttgtgaattt ttcttcagac tctctgtgat gatcatttct
    28741 tgtctttcaa acgtttcttt agctatttta ttatcatgga atgtgtatag tcgatttgtc
    28801 gttccagaaa tcatgtataa agagagtcca ttgaatgacg ttagattttc taaaatcata
    28861 tctagttctt tcttttgaaa accaataaaa atacttcctg actcagctcc tccttgtgtg
    28921 ataggcaact tgatataaaa ggcgtctagt aattttggtg tccccttttt tatttcccca
    28981 cctttgtttt ctctagaaga ttcataatac tccggtagtt gattcaacac gatatatatc
    29041 gcacttagtt cttcataatt tgcgtagaga tctttgattt gatttgggaa agaaactaat
    29101 tcatcggtac tgttttgctg ttcgtataca tacatcagat actgatctat gggttttgtt
    29161 aagtaggtcg tcacattttc gatcttttcg gaattcccta caagctggtt tttgatgatc
    29221 ctactcaagg ttgttttatc attcagcatt ctgcttgtgc tttgaacggc atcacttgtc
    29281 atgttttctg cctgtttttg attttgttcc caagtatgga tactaaaaat aacggtagca
    29341 gtcatcgtta caccgatcat gataaccgcg tatttccgca acagacgatt tagtaaggtt
    29401 ccttttgaaa atagaatccg aaacttattc ataactttcc aactgtttat ctaattcttc
    29461 acgccaatgt gttgtcatcg taccattcca aaccgtcaaa agaccaatga caccaaacag
    29521 gatcaatcct ttgaattgcc aagtcacgcc taaaattata ccgctaccaa taattatttt
    29581 tagaaaagac gaaaagctgg aaaaaaccga aataaaactt agctttagca gattaggaac
    29641 agagatcgta tattcactat ctaaaatcat actgtattga taagtagcaa ataggcagag
    29701 caaggcaaaa agtaaaataa aatcaatagc taaaaaaatg agccctttga tttgaacaga
    29761 taaatataaa ttgtaactca ataaagcagt accagataaa aataaactaa atagcagatt
    29821 gcctcttaaa aaatttcttt tatatatatt ccagcctctt tttattgtga tttctttata
    29881 ctcgaatccg tacaaataaa atagttcatt gactgttttc catgccgcac ctaatccaaa
    29941 gatgatcccg ccggcaaatg tatatatcca gaaatacaag gttagtttta taatgaccca
    30001 gatccggata aataactctt ccaaaacctt ccctaacata tcttctcctc caaacattaa
    30061 gtttatactt tctcattata caaaaaaagg ttgggcaatc agcaccttga cctgattatt
    30121 agagaaacaa acggaaaaat agttcctcat attttgtcgt tttgtgagcg aataccgagg
    30181 gttagctgat tgaacaccgt ttaccaagag tttgtgaaat agccgcttag tttcgagtag
    30241 taagaaaaat tttgtgaata tggctttcca catttttaca tatatttagc ttaataccga
    30301 gaaactggct tttgaacacc gtttattcgg aacaagaggt tgtgacacgt cttttgtcac
    30361 aacccctttg tctttattca ttattattct tgtcggaact catcatattg tttttgcatt
    30421 tctttcaata ccttatcata gccagccttg tccaaagcgt ctttcaattt cggtaatgtt
    30481 tcatctggat caacagtccc tgtattcaat ccatctaaat attgattcat gacattagaa
    30541 atattactta attctgtttt gacactgtca gtgttgaagc tgaatcctaa aatcggtgaa
    30601 gtttctgcat cagcaatcga ttgatcacgt ttggcaatca tgtcatccgt aatagactct
    30661 tgtgtatata gaatcttgtt gtttcctgta ttccaagcag acatatgtgt atttggctga
    30721 taaccatcta gcaatttgat tttatcatct gaaccatcga ctttttccca tgcttctccc
    30781 tcgatccccc aaacaagccc attcaataat tctggatcac tgttaagcag ccctagaagt
    30841 tctactgatt tttctttatt ttttgaagta tttgatacga caaaatttgc catttgtgct
    30901 tgagctgtag attttagtgg aacagtaatt gcttttgaga caagttcttg ttttgctgca
    30961 tttgtcaaaa ttgtatcgcc ataatcatac ggtccttgtg tttctccacg cattagccaa
    31021 gtatttcctt ctagtggata atccttattg ctcgttgcag cgtcacttgg aatgagtcct
    31081 tgtttgtacc agctatgcat tgtacgtaac agtccgatat acgtttcatt atcgtattgg
    31141 ttgatgattt tcgaatcatc tccatccaga tcaatagcaa atggtaacgt attccctaaa
    31201 ggataatcaa agttcccttg gactttgaag ccttgtccaa tggcaaaagc tgccgtgttt
    31261 ggttcttttt catggaattc cttcaagact ttttctgcat cagcgtaaga ttggattcca
    31321 tcaattgaaa gatcgtattt atctagtaaa gctttgttaa aagtaagtac ttgttgagca
    31381 aagacatttc cattgactgg gaaagcatat aattttccat tgaccagatt tcctttaata
    31441 tatgcttcat ctaaatcatt ataagctttt tcagcgtact taggcgctaa ttcagtcaaa
    31501 tcagcaaagg ctcctttttg ggcattggaa acatagttgt tcgcaaaggc gatatcataa
    31561 ttttccccgg aagaaatgat gacgttcatt ttcttttcgt aatcgcccca gccaatgtaa
    31621 ttgatattga cttttacacc gatctctttt tcgattcgtt tgtttgctac ttccatcaat
    31681 tgatcgaaat tatctggttt atctccgatt tgatacatct gcaaagtgtc cgaattgcta
    31741 gcttctgcct ggtctttaga accagaacct ccgcatgctg ctaatgttcc caacaacaac
    31801 gctgaaacac ctgtaaaggc tagtttcttc cataacttca tttttcttcc tcctaatttt
    31861 atgatttata tttttgtttc tttattcttt tactccgccg attgttagac ctttgacaaa
    31921 atgcttctgg aaaaatggat agctgatcgc aataggcaac gtcgagatga ctacgatcgc
    31981 cattctggca gattctcctg gaactgaggc taatccgcct gtcaattggc ttcctgctcc
    32041 tgaattttgt gtcagatatt gaatattact ttggattttc atcaaaagat actgtagtgg
    32101 aactagctta tcatcttgga tgtatagtaa agcattgaac cagtcattcc agtaacccaa
    32161 tgcggcaaat aaactgattg tcgcaatacc tggaacagct aacggtaaaa cgatctgaac
    32221 aaatattcgc agttcgctag ctccatcgat ccgtgccgat tcaataatcg aatcagagac
    32281 cgaacgttta aagaatgtgc gcatcacaat aatattgaag ggactgacag ccattggcaa
    32341 gatcaatgcc caaatcgtat cttttaactg aagaagattt gtcataacaa gataattggc
    32401 caccatccct ggtgagaaca acatcgtaat caaacaaaaa acggtaaaaa aacgacggaa
    32461 tggaaagttt gaccgtgaaa tcgcataagc atataaagaa gtagccgtgc tattgatcag
    32521 tgtacctaaa acagtaacaa gtacagtgac gaaaagcgct tggaaaattt tatcctgcat
    32581 ctggtcgaac aagtacgtat aaccaaatat actgaacttt tctggccaaa aactgtaccc
    32641 gtgagtcgac aatatggact catcggtaaa agagatggcg ataacaaata taaatggcaa
    32701 aatacaggaa atagcaaaga gtgcaatcat tatattagag aaaaaattag ccgtcttatt
    32761 gaaagagcgg acttggactc tctccacttt cttttttttc atttttttcc tccttagaat
    32821 aatgcagaat cgctatcaaa acgtcgggcg atcgtatttg tgatcatcaa caagacaaag
    32881 ccgacaactg attggtaaag tcctgcagca gccgtcatac caaaatctcc tgtggatgtc
    32941 aggccattgt agatatacgt atccaaaact tgagtcacat catataatgc accggaattc
    33001 ctaggaatgt tataaaacag gccaaaatca gctcggaaaa tgtttcctac ggctaaaatc
    33061 gttaaaatcg tcattagtgg aataagttgt ggtatcgtca catgtttgat ctgctgccat
    33121 ttgctagctc catcaaccat agcagcttca taataagtcg gatcgattcc cataacagat
    33181 gcaaagtaaa taatgctgtt ataaccaatc cctttccatg taccaatgaa caccaagata
    33241 aacggccaat atttcggttc attataccaa ttgacagctt ctcctccatg tccagtgatc
    33301 cactgattga atattccttt gtcaggactt aaaaaagcgt aaacaaagta actgataatg
    33361 acccaagata gaaaataagg tagtaaggac atcgtttgat aaactttaac gagtcgttta
    33421 tttctcaact cgctcataat gattgcaaat acgactgaga tcagcagatt caaaaggata
    33481 aatgctacat tatataaaat tgtattttta gtgatgagaa atgcttgatc cgaagaaaat
    33541 aaaaatttga aattttccca acccacccaa ggactttccc tcaagctagc caagaaccca
    33601 tctgctgaaa tatgaaaatt tttgaatgct acgacattcg ccattacagg tatgtaaaag
    33661 aaaaaaatca tccaaagcat acccggtaca gccatcagga caagtgcttt ataacgccat
    33721 agattcataa aaaaatgatt gaaccccttt cgttttttca tctaaaaacc cctttcgatt
    33781 cattcgcaac gtaattatag cgctttcaat aatgagaata aataaacaaa gtatagggaa
    33841 aatgaaacaa agtataggag aggttgtgaa atcagcgttt tggcctgagt attagagaaa
    33901 aaaacgggaa aatagttcct catattttgc cgttttttga gctaataccg agggtcagct
    33961 gattgaacac cgtttatcag aggttgtgaa ttcagcgttt tggcctgagt attagggaga
    34021 tcgtgtcctt actatctaac tctgagtcac aaggaacaat ggggactgta acaaaagtct
    34081 tgtcacagtc ccttattccg aacaaacggt gttccagaag tagcttctac ggaaataagc
    34141 cgaaatttca caaaaaagcc atccgaaagt cagacggaca agtctgcttt tcagatggcg
    34201 ctacaactac tctgcttatt cttttagcta tccattcaat tatttttctc tcacttgttc
    34261 tagctcttct ttggtcatgg gtcgatattg ccctttattc aaatctttag gcagccaaaa
    34321 ttttcccata cggattcttt ttaaatataa gacttctttt cctactgctt tgaccattcg
    34381 cttaacttgg tgaaactttc cttcatgcaa aatcagacgg ataattgaag tttctgtctc
    34441 ttgatctact tgatcaattt ggagttttgc cggcaatgtc tgctcatcgc catcaataat
    34501 cagtccttct gcaaataaac ggatatcttc ttctgtcatt ataccgctaa cctcagctag
    34561 atattctttc tccacatgtt ttttaggtga tagtaactga tgggcaaaca ccccgtcatt
    34621 cgttaaaatc aagaggccct ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc
    34681 ccgataatcc tggtcttcca ataaatcgat tacggtttga tcatgattat cctgagtagc
    34741 agaaatgact cctgcaggtt tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat
    34801 ttctcctaaa taagtaaccg catcttcgaa ttcatttact tgcgctttat cgcttttcac
    34861 cacttgtcca ttgacttcga tcgttccttt tttgattaat cctttgactt ctttgcggct
    34921 gccaagtccc atatctgcta aaaacttatc taaacgcatg cgtttccccc atttcaaaag
    34981 agcatcaatc tagctcttct gattcactta ttttattttt aatttatgtc gtaaacgcgc
    35041 tattcctggt cctagtagtc tttctgccaa gcggattttc aaagctaaat aaccatacac
    35101 taaagcccct atacctgcta ctacaagaat caacaataga gactggaatt tgctatcttc
    35161 gtttaaaaaa gcaccaaaaa tcatctttgt gatccacgaa gccaaaagca taatcaaggt
    35221 aagaataaag atcagcaatg tcctgcgcca gacaaaactt ggtttaaagc gggctacttc
    35281 atggattctc tttaagatca aggtacaaga cacagcaaat ccgatcattg tcgccaataa
    35341 agggccgtat acttcaaata aacgaatggc tgggtattga agaaggagtt tcactagaaa
    35401 cccaataacc aaataattaa ttgccgcctt gttttcaaac attccttgca gcatgttgga
    35461 aactagcata tatagtccta ggaagagtcc tacaaaactt gcttggatca gtacattact
    35521 tcctagagaa tccggtgtat aaaataatgt atttagcgga taggcaagta acataacacc
    35581 aaatgtcgcc ggaaacataa aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc
    35641 cagacctact cgatctttca acgtaacagc ttctgtaatc aacggtaacc cagtagacgc
    35701 gatcgatgtt gccaatgcaa taactaccat cgtcaactta tctggattgg cactaaaaat
    35761 cgaaaaaaga tccagtaact gtgcattgga atactcggtg gatgcagaca tgatcttcat
    35821 aaaagtgaac tgatcgacta atttgaagat cgttacccct gaacccacaa taataaacgg
    35881 aatcgcttct ttaacggtat ctaacaacaa ttccttcgtt gcaatattta ttttgttttc
    35941 gctgtagtga ataaacgccg atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac
    36001 aagaatactt gctagcatac cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac
    36061 atagtcacca tccatgactt tcatgatgat gaatgtagcc aatagcatat aaaaaatacg
    36121 tgccacctgt tctacaatct gcgacagcgc ataaggcatc atttcttgat tgccttgcaa
    36181 ataccctcgt atcacactca tacaaggaaa aaccaataca gcgatactca aagaacgcat
    36241 cgttggaatc agttcttctc cgccaccaga agcatgagct agccaaggag aggcgatgta
    36301 catgaacaaa gcaaacaaca ggcctaaccc gcccatcatc tgtaatgcac ggataaataa
    36361 tcgtcgtgaa gtgccatatt cattcaatga attatagcgt gcagtttgtt ttgcaatcgc
    36421 agcaggtatt cctgctgtag agatcagtag aaacaatgca tatatattgt atcccatatt
    36481 gaataatcca ttggcagcct tagcattttc gcccatccaa gcataccagg gaatgatata
    36541 gacagcgcct agcaatcttg aaatgatatt actggcggtc atccaagcag agcctcgtgc
    36601 cattttttct tgcggtgtta attgatctaa tgctggtttt ctttgattat ccatcggtca
    36661 gattccaact ttctacaaaa cattactatc attttaattg gtaaaacaga aactggcaat
    36721 cgattttata taacttttat aagaacaatt gtttttttga attcggtttc gtttttttgg
    36781 taaagtaaag aaatatacag ccttttatgg ctaacattta taggagatat caatgacaca
    36841 agaaataaaa aaactacccg ttatattttt aggtcttttc tttatcacag ttggattgaa
    36901 ttggttcttg ctccctcacg atatagcttc cgccggagtg ggcgccatcg gacatttagt
    36961 tgaaacggcc ttttctatcc aacgctcagt tactgtttgg agcatcaatc ttatgatgct
    37021 gcttctagcc gctgtcctat taggaaaatc agtatttttt aagactgtag cgggaagtct
    37081 gctttttcct gttgttttag agattgttcc aaaagtggaa atgatctcct cacatttctt
    37141 ttcactgatt tcaggaagtc ttttgttcag tcttggggtt tacacattgt acacagtcgg
    37201 ggcctcaaat ggcggtataa cgataccgcc gattattttc cacaaatttt ttcggcttcc
    37261 aatgccgcaa ggacttttac ttaccaatag tcttatcgtc tttttgaatt attatgtttt
    37321 tggattgttg gagacactat tcgttctcct atctattaca gtcagttcat tgtttatgaa
    37381 aatactcatc cgcttgtccc ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata
    37441 gcggaaacaa acgatatatt aaaaagagtg tgctataata aaccaagaaa ttgaataaag
    37501 cgagggatca atcaatgagc ttctctttag aagacatccg tcatttacta ttaaaagaaa
    37561 atctattaaa agaatttgtt tcaacaagag gctggcatct tgatgtacca gaaaaagctg
    37621 aatttcttca gttaagttat gattcaagaa aagctgatgc ttctactttg tttttctgta
    37681 aaggaatgaa tttcaaagaa gaatatttgg attcagcaat cgaacagggt attcaatatt
    37741 atgtatcaga acaaccatac gaaaattcag cagtaggaat catcgtaacg gatatccgta
    37801 aagcaatggc gcttcttgct atggcttttt acgactatcc gcaaaataaa ttgaaagtga
    37861 tcggattcac tggaacaaaa ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg
    37921 atcacacaac aaataaaaaa acagcattgc tttctactat gaatacaacg ttagatggca
    37981 aaacatattt caaatcccat ttgacaacgc ctgagtcatt agatttatat cgtatgatgt
    38041 cagaagcagt tgaaaatggt atgactcatc tagtgatgga agtttcttca caagcttaca
    38101 aaacacaacg cgtatacggc ttgactttag atgtcggaat cttcttgaat atctcgcctg
    38161 accatatcag tccgatcgaa catccgacat ttgatgacta tttctattgc aagcgacaat
    38221 tgatcttgaa ttcaaaaaca gttgttttaa atcatgaaag tgactatttt gatttactta
    38281 gggaaacagc cgatctattc cagattccaa cgatcactta tggtcgttcg gaagaaagca
    38341 attatcaagt gatccgttca gataaaggta ctcacggttt tactttatct tctcatgaag
    38401 atcgcttagc tatctgcgat actgcttatg atattttgct ggctggagga ttcaaccaag
    38461 aaaatgcagc cagtgcgatc attgccgcag cactaacagg tgcctccagt caagatgcac
    38521 aagacggatt aaaagaagct cgtgtaccag gccgaatgga tcaattgatc caatccaatg
    38581 gagcgcatgt ctatgtggac tatgcacata attatctaag cttgaaaaca ttgctgaaat
    38641 ttgccaaaaa tgaacatcca gatggccgtg taattgttgt attaggcagc ccaggaaaca
    38701 aagcaatctc ccgtcgacat gattttggaa aagtgctatc agaaacagct gacatcgcct
    38761 ttttgacggc agatgaccca gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg
    38821 aagcaattac taatccagat ttgatcgttc attatgaaat ggatcgtcct gaagcgattc
    38881 gacgcgcttt agcagaaagc gctccgcaag attcagtcgt tattgcggga aaaggcgtgg
    38941 atccctatca aaaaatcaat ggtgtagatg aaccttatga aggggattat gcaatcgtga
    39001 agcggttgat tgaagaataa tggtatttat atactagtgg tttcccattt ctaagaaaaa
    39061 aagggactgt gattttttgt cacagttcct tttttcttag aaatcatttt aacttttcac
    39121 tagctaattc tgctgccttt tctctttctt catttaataa tttattatca tagaatttga
    39181 taaatgggaa ccagataatg aatgcgatga ttgcacaaat gactgctaac acagcagctt
    39241 tccaatcacc accactacca acaaatgctc cgatcccgac tggtgaaggc cacggcatct
    39301 gtgcaagcat cggtttgacg atttccagcc gaatggcaaa ataagcgagt gtagcagaag
    39361 ccatcggagc taagaaaaac ggaaccgcta aatagggatt gtagacgatt ggcatcccga
    39421 aaatgattgg ttcattgata ttgaataatc ctggtacaag tgaggctttt cctaacactt
    39481 tcagctgatc agatttcgcg aggaatgcaa taaagatgat caatcctaaa gtcgcacctg
    39541 atccaccaac ggttacataa gaattgttga attctcctgc caacggataa tttgcgcctt
    39601 gtgcgttagc tgccatatta gctaatacga tcggggtcaa gaaagaagtg atgatattcg
    39661 ctccatggat ccctacgatc cacaatgcat ggatcaagaa ataaatcacc atgacaccaa
    39721 gccatgtatt cgtcaactgc gtaacaaacc caaatgggat agcaatcatt ttgaagatat
    39781 ccgttcctag tgcaactaat aatccattga tgatcatcac cacaaacgca atgacaaatg
    39841 ttgggatcag agcagtgaaa gaacgagaaa ctcctggcgg aacaacatct ggcattttta
    39901 tgacccaatt tctcttgaca cacatacaat acaattcgac cgcaataatc gacataataa
    39961 tagctgtaaa gattcctgaa gttcctaaac gttcgacaaa agagcccatc ctaataccat
    40021 ttactacagt ttctgtctca ttgatagaag caatcaagct gattttacca tcagtcatga
    40081 tcaattcagg gatacacata aagaatgcaa aaacagatag taatgccccc gtgagcggat
    40141 taacatttaa tttttgttca tctcgttcaa ttgacgttaa ttcataagca aacacaatgg
    40201 caaagtaaat agataaaatc cccatcgtca ttgtattgac gatcatgtac aagtcactaa
    40261 ctttgaaaaa agttgcatta aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat
    40321 ttaaaaccaa aaacatcgag cctacaatcg tgaaaggaat agaagccatc cccgctgcca
    40381 tgacagcccg aacgatttta agttgcgcaa ttttccccat aggtcccatc aggtatttct
    40441 gcaaaaaatc aaacattagt tattctcctt tcctaaatat aacgcttttt catattgttt
    40501 aatacggata tactgttggt cttggttgag tgaaattttc tttaaacgat actgtataaa
    40561 tgcactggca aaaataccgc ttacaacaag aatgaaaaca aataactgtc ctccattgtt
    40621 atttgccata aaaggataca agaatgtaaa tcctgaggta aacaacactg gaatcactaa
    40681 taaattaaag acactctgga acaaaaaata ccactttgtc catggaacgt tcgtttggtg
    40741 ttttcgataa agctttactt gttctcctac tgcaggcaaa atagctaaaa tcagaatcat
    40801 cggtaaacta cttcctaaac ttttagctga tagaagcaga atcatccatt gcatgttcat
    40861 gaaaaagaaa aaggcagtca ggtaacgtac gattagataa cgattgaaat acatgttttt
    40921 caaactaagt tgcttttttt ctaattcgat tttccttttt actgtatcct gcacaatgat
    40981 ccttcctttc tatttatttt tttgatacaa ataattcatt tcctgtgcaa cttccaataa
    41041 tgtcatcgtc gtcatcagat gatcttgtgc atggaccatg ataatttcca tttctatttt
    41101 tgtcccgctg gcatattctt tcagaagttg agtttgtgac tgatgcgcat gaagaatctc
    41161 ttcatttgct tgtcgcaact tctcttctac ttcttcaaac atctctttac gcatcgagcg
    41221 aaatgcttca tgtatcagtg tccgtgcatt tccgctatgg aaaatgatat caaaagaagt
    41281 cacttgtagt gtttcactgt ttattttttc agccatctgt tttcctcctt attcgatcag
    41341 tttgataaat ttactgctga aattctcaaa aataggttca gctaagattt cctgctgtac
    41401 ttttagctgg tcaactaacc taacgattgc tttagtcacg accgtaattc cttcattttc
    41461 gacataagaa ggcgaaacta aaaaaacaaa atgaatttct tgatattgat cccactgcat
    41521 cccagaagga atcaatgcaa cggctacctt tgtttcgatt ccaactggaa cagcgggatg
    41581 tgggactgcc actgtatcac tgaatatgat ttgtcccata ttttcacgtt gctggatctg
    41641 ctttcgcatt tctcttatat atccttcttc ttcatttatg ctcagctttt gcagcaaatc
    41701 atgaagaatt tcttcctttt tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa
    41761 ctgttcggtg taaatctgct ttttttgctg tagagaaaat gctggtatcg gtctatctga
    41821 ggaatgatag ggtatagatt ctccaactac ctttcgtatt ttacgaacat catcttcatt
    41881 caaaaaaata ctgacatgta aaactggaat tttgaaaaac attgtcgaaa gatcaataga
    41941 agaaataatc aggtcgattc cttttaaagt tgtttcattg atctcataat agccttttac
    42001 acttaccact gtaatatttt taccgaattc actgactacg cgattcttta atagttgggc
    42061 actgccgtaa cccgttgcac agataatcaa tgcatgtagt ttatgcgcag ctctttcttt
    42121 ttccaatgcc gccatgagat gcaaagccaa ataggcccat tcatcttcat tgatggtata
    42181 gtttttcaaa gccggcatgt tgctgaaatg cctttttact gcgtcaaaga ctttgggatt
    42241 ttctttcttt atctcctcag tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat
    42301 catcatcggt gacaaatgat cgagtagtcc attgaaaagc tggagatcct ctactaatga
    42361 aaaatgaaat tcctgagaca atcttttcag aactttcgct aattcctttg atagttcctg
    42421 atcattttga ttttctttgt gattcgactt agccatgaga tgtaatgtca aataagcaat
    42481 ttcttcttcg ggaaacgata cacctgcgat tacctcaata cgttgaacga tccgctgcgc
    42541 cacttgatac tctactcgct tagaaacttc ttgttcgatg cctaattcct tgatttcaaa
    42601 cccttctcgg agccgtttga tccccaaagc caagtgtaaa gtcagattct gtatgataaa
    42661 atccgaaact tttaacttag cttctcttgt ttcatcgaga atgataatga ctagctcttc
    42721 aaaactgatg tctgggaaaa aacggctttg ccctaaatat tctttcaatg aatttccata
    42781 atggtttcca aaaaaagtat ccatgataaa atgccgtcga tttctttctt ccccttcgat
    42841 ccatgtccct tgaccatgct tagaaacgat ttttagtgaa tatggaatca atcgctcctt
    42901 gatttcttgc atgtcctttg tcaaactgga acgactgata taaagctctt ctgccaaatc
    42961 atccataaaa agaatggcat cttccaaaag caattggttc aatatatact tttgtcgatc
    43021 ttcggactca taaaatcctt gcggttctct agcatatcca ggtactattt cttgccgact
    43081 caaaaacaag tcaaatgtca gtctttgtaa aatatgcagt tggtagccat aacctggttt
    43141 agcaatgatc ttacctccgt ttttttctac taactctttg atccgctgca agtaattacg
    43201 gattgtccga tcagataata acaattcaga agctaaacgt tgactggtga catactcctt
    43261 cttgtgatca atcaaatatc tcaacagcaa tttttccttc tgtttcatta tcatccactc
    43321 ctattccatc atatgttctc ttcgatcacc tctgctaact tctggatgcc tgtagggatt
    43381 gggacatacg cttggaaagg gatactaaca acaggctttc caacagattc tcctaatttt
    43441 ttgaatttat ccaaaaacat cgtcgtttgc gggctgatca gaaataaatc aaaatcacta
    43501 ttcttaatca tattatcgcc ttctgtagct gaaactgcat ctacgagtac ctcttctctt
    43561 ttttgcttaa agaaatccgt tgtctttgag gccatcatcg aggatgacat tcctgccgca
    43621 caaataatca atgctttttt cataaatcaa tcactccttt tatttttata aaccaagcat
    43681 atcttgtaag cgttttctat tcaaaatata tatttccgta gcattgcgga aaaaatgtgt
    43741 atcatatctt gaataataat ttctggcacc gtgatatact agtgatagaa ttttttcagg
    43801 aggggattta gtgagtatca cagtgaatat tttgtataca agtacaaatg gacaagccag
    43861 aaaatttgca gaagaaatgg tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg
    43921 aaacgagaaa tatgactatt actttccact agaagatcca gaatctcttt tactgatcga
    43981 ccgttggaaa gatgaagaag caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat
    44041 aaacgaactc cgtaaaaagt ataaattaaa aatgaaagtc gaacgtttca ctgatttacc
    44101 gtaaaaagat acacatggaa tgatagcaat ttatcatcca tgtgtatctt cactttttcc
    44161 ttatttttta ttcgatctca tctaatgatt caaaccgtgt ttttctctta tttttcggat
    44221 aagtatttca gtcaccccaa tatacatgat tcctaaacat accccaagga tattctcaaa
    44281 aatgcggatc gttactgctc ctggaacccc gaataaactg gcagtgattg ccaaagcacc
    44341 aaaagaatta aaaactgttt tatatctata ggtagaacaa acacctaaag ctagaccacc
    44401 taatataccg acccagttaa aaggaataaa ttggctgata ccaataaata aaacacatcc
    44461 aatcaatgag ccaatgattc ggtctactgc tcgttctttg aaaccaatcg acatcaatcc
    44521 atagctagat acaatagaag aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac
    44581 atactctcct gcaacgatca aaagactaat gccaaatgca tagatgagca tccaaatttt
    44641 ttcctgcgaa tacatgcctt tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt
    44701 ttctcgatgt tttctgtaaa ggagaactga aaaccaacaa aaaaacaaaa ataataagct
    44761 acttgtttgt gtaaagaaat tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa
    44821 gagatatgaa aagccgtaca aagaagcatt ccccattttg ggatcactag cggttgccat
    44881 taatatgctg cttagtaaaa taaaatgaat caacatgccc ataactgacc atttgatcag
    44941 ctggacatac ggtgctacta atagtgagaa catgacgatt gctaaaccta tgatcgaatg
    45001 ggaaatctta taaccaaagt caacaaaacg aatactcatc aacatacaga ataaaaccac
    45061 agcgtatggt ttatgcttcg attcaaataa tgttgtaatt ataatgataa aacaaatggc
    45121 aaacaatacg atgaataatg atcgtaaaaa caatgaacaa atatacatgc acttttcttt
    45181 tttagaagct gcttctcgta ttttttgttt taaaataaac ggatctagtt gtagagactg
    45241 ataaaaatcc acctgttgtc gtcctttctt tttactttta ttttttacag tcatctatga
    45301 caggttgtaa atactatgga acatcatatg gcgttgtcat tttcttgtca actatttttc
    45361 ctattcatgt ttttataaaa aaacatatcg ttttcttgta tgattaaatc accacaaaat
    45421 aaacaactga gagaacgata tgctttatga atggttctat caaaaaaatt ctgagatcaa
    45481 tagacaaaaa tttgatgatt acagaggtct cttacgaaac cttacagaag aaaaaaacat
    45541 tgatcgttgc tgcagttctc ttccctgctc catgtgtctg tagaaattgt ggttctgctg
    45601 tggtagatgg aaataggaaa ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc
    45661 actttgaata atataatcat atgccaatgg ttatacgcct aaaaaagcag cgctatacct
    45721 gtaaaaactg tcgaagccat tggaacgctc aaagttattt tatccgtcca aggcactcga
    45781 tttcaaatca tgtcagacat aaaatcactt ctttactgac tgaaaaagtg tcgttatttt
    45841 ttatttcgaa aagttgttag gtatctttga caaccgttat tcgcacattg aaagagttga
    45901 gaagctattt accaaagtct tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt
    45961 tcgttctcac gtttccgttg aggataaact gagttttatt tccgcggatg gcgagacaga
    46021 aaaattaatc gagaaaattt acctacacaa aaaattcctc gattaacaaa ctatttcctt
    46081 agctgcacta atcctaaaga aatagaattc ttagttacag acatgaacgc cacctacttt
    46141 cagctcacca aacatgttct accaaatgcg aaattggtga ttgatcgatt acttggtttt
    46201 tccacgcctt tcaaggaggc gtaaccttta ttcacgagtt ggttgaagct ttccgagaca
    46261 aaagacccga cttgtttttc tccttattga caaagagcca ttaaaactag agaaaaaatt
    46321 acaactgatc gctgattatt tgtagaaaga aaatcaaagt attacccaat aaacttgagt
    46381 cattaggcaa aaaacactgc gaaactcaag aatgattttc gcagtgtttt tgtatatttt
    46441 ttattaacta gtattactac aaaggtttat ttttcacttc tcaacgcagc acaaaattgt
    46501 ctttacttat ttactggttg ggttcaacct tctctactcc gatactaata tttgtttgta
    46561 taccccttct aaaatcggta gtttccattc aattgatggg tactagttga ttttaaaaac
    46621 tatgcttatt caatttttta ttttagctca tatatttcgt ttctttctaa attttatact
    46681 gatgaaaaca agtagcacac ccaatattga caatataaac tggctaatga tggtttctcc
    46741 agtttttgga agatgcttat ttccactgct tgttggattg tttcctctta tatctgagct
    46801 atcagcaact tttctattct cttttgttaa aaagatcgcc acttcttgtt ctttttcgat
    46861 cttgaaagtt actggtgtcg catccaattc ataccccgtt ggcgcttgag tttctaccag
    46921 ctgatatgct cctggtgcta agccatctat cgccagtttt ccatctgctc ctgtggttag
    46981 acctgactgc agaacggttc cattggcatc ttgtaactca aaaacagctc cttgcaggac
    47041 ttctccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa gtcgattttc
    47101 tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga ttggtgtcgc
    47161 atccaattca taccccgttg gcgcttgggt ttctaccagc tgatacactc ctggtgctaa
    47221 gccgtcaatc gccagttttc catctgctcc tgtggttaga cctgactgca gaacggttcc
    47281 attggcatct tgtaactcaa aaacagctcc ttgcaggact tccccgcttt ggtcatcgat
    47341 ctttgtcaac acgacaccgc ctggtgtaag tcggtttgct ttcgtcaatt ctaccaccgc
    47401 tgtctgactt ctttcgatct caaactcgat cggtgtcgca tctaattcat acccc
//
LOCUS       DAYLZO010000008        42613 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.008, whole genome shotgun sequence.
ACCESSION   DAYLZO010000008 DAYLZO010000000
VERSION     DAYLZO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.008"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..831)
                     /gene="guaA"
                     /locus_tag="ACGVJG_000395"
     CDS             complement(<1..831)
                     /gene="guaA"
                     /locus_tag="ACGVJG_000395"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFJ6556382.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     F"
     gene            complement(890..1813)
                     /gene="coaA"
                     /locus_tag="ACGVJG_000396"
     CDS             complement(890..1813)
                     /gene="coaA"
                     /locus_tag="ACGVJG_000396"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFJ6556383.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2009..2614
                     /locus_tag="ACGVJG_000397"
     CDS             2009..2614
                     /locus_tag="ACGVJG_000397"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFJ6556384.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            2922..3938
                     /locus_tag="ACGVJG_000398"
     CDS             2922..3938
                     /locus_tag="ACGVJG_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6556385.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4079..5380
                     /locus_tag="ACGVJG_000399"
     CDS             4079..5380
                     /locus_tag="ACGVJG_000399"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFJ6556386.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            5426..6088
                     /gene="deoC"
                     /locus_tag="ACGVJG_000400"
     CDS             5426..6088
                     /gene="deoC"
                     /locus_tag="ACGVJG_000400"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFJ6556387.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            6109..6507
                     /locus_tag="ACGVJG_000401"
     CDS             6109..6507
                     /locus_tag="ACGVJG_000401"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFJ6556388.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            6573..7655
                     /locus_tag="ACGVJG_000402"
     CDS             6573..7655
                     /locus_tag="ACGVJG_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFJ6556389.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            7867..9429
                     /locus_tag="ACGVJG_000403"
     CDS             7867..9429
                     /locus_tag="ACGVJG_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556390.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            9422..10555
                     /locus_tag="ACGVJG_000404"
     CDS             9422..10555
                     /locus_tag="ACGVJG_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6556391.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            10555..11511
                     /locus_tag="ACGVJG_000405"
     CDS             10555..11511
                     /locus_tag="ACGVJG_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6556392.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            11610..12776
                     /gene="deoB"
                     /locus_tag="ACGVJG_000406"
     CDS             11610..12776
                     /gene="deoB"
                     /locus_tag="ACGVJG_000406"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFJ6556393.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            12800..13618
                     /locus_tag="ACGVJG_000407"
     CDS             12800..13618
                     /locus_tag="ACGVJG_000407"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFJ6556394.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            13635..14342
                     /gene="deoD"
                     /locus_tag="ACGVJG_000408"
     CDS             13635..14342
                     /gene="deoD"
                     /locus_tag="ACGVJG_000408"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFJ6556395.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(14640..15326)
                     /gene="gpmA"
                     /locus_tag="ACGVJG_000409"
     CDS             complement(14640..15326)
                     /gene="gpmA"
                     /locus_tag="ACGVJG_000409"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFJ6556396.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(15498..16079)
                     /locus_tag="ACGVJG_000410"
     CDS             complement(15498..16079)
                     /locus_tag="ACGVJG_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFJ6556397.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(16212..16895)
                     /gene="rpiA"
                     /locus_tag="ACGVJG_000411"
     CDS             complement(16212..16895)
                     /gene="rpiA"
                     /locus_tag="ACGVJG_000411"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFJ6556398.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            17214..17627
                     /gene="rpsL"
                     /locus_tag="ACGVJG_000412"
     CDS             17214..17627
                     /gene="rpsL"
                     /locus_tag="ACGVJG_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFJ6556399.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            17711..18181
                     /gene="rpsG"
                     /locus_tag="ACGVJG_000413"
     CDS             17711..18181
                     /gene="rpsG"
                     /locus_tag="ACGVJG_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFJ6556400.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            18275..20362
                     /gene="fusA"
                     /locus_tag="ACGVJG_000414"
     CDS             18275..20362
                     /gene="fusA"
                     /locus_tag="ACGVJG_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFJ6556401.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            20534..21721
                     /gene="tuf"
                     /locus_tag="ACGVJG_000415"
     CDS             20534..21721
                     /gene="tuf"
                     /locus_tag="ACGVJG_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFJ6556402.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            22128..22436
                     /gene="rpsJ"
                     /locus_tag="ACGVJG_000416"
     CDS             22128..22436
                     /gene="rpsJ"
                     /locus_tag="ACGVJG_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFJ6556403.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            22467..23096
                     /gene="rplC"
                     /locus_tag="ACGVJG_000417"
     CDS             22467..23096
                     /gene="rplC"
                     /locus_tag="ACGVJG_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFJ6556404.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            23124..23747
                     /gene="rplD"
                     /locus_tag="ACGVJG_000418"
     CDS             23124..23747
                     /gene="rplD"
                     /locus_tag="ACGVJG_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFJ6556405.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            23747..24037
                     /gene="rplW"
                     /locus_tag="ACGVJG_000419"
     CDS             23747..24037
                     /gene="rplW"
                     /locus_tag="ACGVJG_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFJ6556406.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            24076..24909
                     /gene="rplB"
                     /locus_tag="ACGVJG_000420"
     CDS             24076..24909
                     /gene="rplB"
                     /locus_tag="ACGVJG_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFJ6556407.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            24952..25230
                     /gene="rpsS"
                     /locus_tag="ACGVJG_000421"
     CDS             24952..25230
                     /gene="rpsS"
                     /locus_tag="ACGVJG_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFJ6556408.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            25253..25600
                     /gene="rplV"
                     /locus_tag="ACGVJG_000422"
     CDS             25253..25600
                     /gene="rplV"
                     /locus_tag="ACGVJG_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFJ6556409.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            25614..26270
                     /gene="rpsC"
                     /locus_tag="ACGVJG_000423"
     CDS             25614..26270
                     /gene="rpsC"
                     /locus_tag="ACGVJG_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFJ6556410.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            26273..26707
                     /gene="rplP"
                     /locus_tag="ACGVJG_000424"
     CDS             26273..26707
                     /gene="rplP"
                     /locus_tag="ACGVJG_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFJ6556411.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            26697..26885
                     /gene="rpmC"
                     /locus_tag="ACGVJG_000425"
     CDS             26697..26885
                     /gene="rpmC"
                     /locus_tag="ACGVJG_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFJ6556412.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            26910..27176
                     /gene="rpsQ"
                     /locus_tag="ACGVJG_000426"
     CDS             26910..27176
                     /gene="rpsQ"
                     /locus_tag="ACGVJG_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFJ6556413.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            27234..27602
                     /gene="rplN"
                     /locus_tag="ACGVJG_000427"
     CDS             27234..27602
                     /gene="rplN"
                     /locus_tag="ACGVJG_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFJ6556414.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            27639..27947
                     /gene="rplX"
                     /locus_tag="ACGVJG_000428"
     CDS             27639..27947
                     /gene="rplX"
                     /locus_tag="ACGVJG_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFJ6556415.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            27975..28514
                     /gene="rplE"
                     /locus_tag="ACGVJG_000429"
     CDS             27975..28514
                     /gene="rplE"
                     /locus_tag="ACGVJG_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFJ6556416.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            28533..28718
                     /locus_tag="ACGVJG_000430"
     CDS             28533..28718
                     /locus_tag="ACGVJG_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFJ6556417.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            28754..29152
                     /gene="rpsH"
                     /locus_tag="ACGVJG_000431"
     CDS             28754..29152
                     /gene="rpsH"
                     /locus_tag="ACGVJG_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFJ6556418.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            29184..29720
                     /gene="rplF"
                     /locus_tag="ACGVJG_000432"
     CDS             29184..29720
                     /gene="rplF"
                     /locus_tag="ACGVJG_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFJ6556419.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            29881..30237
                     /gene="rplR"
                     /locus_tag="ACGVJG_000433"
     CDS             29881..30237
                     /gene="rplR"
                     /locus_tag="ACGVJG_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFJ6556420.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            30258..30758
                     /gene="rpsE"
                     /locus_tag="ACGVJG_000434"
     CDS             30258..30758
                     /gene="rpsE"
                     /locus_tag="ACGVJG_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFJ6556421.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            30773..30952
                     /gene="rpmD"
                     /locus_tag="ACGVJG_000435"
     CDS             30773..30952
                     /gene="rpmD"
                     /locus_tag="ACGVJG_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFJ6556422.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            30996..31436
                     /gene="rplO"
                     /locus_tag="ACGVJG_000436"
     CDS             30996..31436
                     /gene="rplO"
                     /locus_tag="ACGVJG_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFJ6556423.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            31436..32731
                     /gene="secY"
                     /locus_tag="ACGVJG_000437"
     CDS             31436..32731
                     /gene="secY"
                     /locus_tag="ACGVJG_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFJ6556424.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            32793..33440
                     /locus_tag="ACGVJG_000438"
     CDS             32793..33440
                     /locus_tag="ACGVJG_000438"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFJ6556425.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            33631..33849
                     /gene="infA"
                     /locus_tag="ACGVJG_000439"
     CDS             33631..33849
                     /gene="infA"
                     /locus_tag="ACGVJG_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFJ6556426.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            33882..33998
                     /gene="rpmJ"
                     /locus_tag="ACGVJG_000440"
     CDS             33882..33998
                     /gene="rpmJ"
                     /locus_tag="ACGVJG_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFJ6556427.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            34016..34381
                     /gene="rpsM"
                     /locus_tag="ACGVJG_000441"
     CDS             34016..34381
                     /gene="rpsM"
                     /locus_tag="ACGVJG_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFJ6556428.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            34409..34798
                     /gene="rpsK"
                     /locus_tag="ACGVJG_000442"
     CDS             34409..34798
                     /gene="rpsK"
                     /locus_tag="ACGVJG_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFJ6556429.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            34878..35816
                     /locus_tag="ACGVJG_000443"
     CDS             34878..35816
                     /locus_tag="ACGVJG_000443"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFJ6556430.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            35844..36224
                     /gene="rplQ"
                     /locus_tag="ACGVJG_000444"
     CDS             35844..36224
                     /gene="rplQ"
                     /locus_tag="ACGVJG_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFJ6556431.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            36496..37335
                     /locus_tag="ACGVJG_000445"
     CDS             36496..37335
                     /locus_tag="ACGVJG_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFJ6556432.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            37311..38180
                     /locus_tag="ACGVJG_000446"
     CDS             37311..38180
                     /locus_tag="ACGVJG_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFJ6556433.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            38170..38967
                     /locus_tag="ACGVJG_000447"
     CDS             38170..38967
                     /locus_tag="ACGVJG_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFJ6556434.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            39170..39916
                     /gene="truA"
                     /locus_tag="ACGVJG_000448"
     CDS             39170..39916
                     /gene="truA"
                     /locus_tag="ACGVJG_000448"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFJ6556435.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            40580..40774
                     /locus_tag="ACGVJG_000449"
                     /pseudo
     CDS             40580..40774
                     /locus_tag="ACGVJG_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(40962..41738)
                     /locus_tag="ACGVJG_000450"
     CDS             complement(40962..41738)
                     /locus_tag="ACGVJG_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFJ6556436.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            41861..42268
                     /locus_tag="ACGVJG_000451"
     CDS             41861..42268
                     /locus_tag="ACGVJG_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFJ6556437.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        13743 a         7774 c         9171 g        11925 t
ORIGIN      
        1 gaattttccg cctagacttt ccattacttg ttccgcttcg cctttgcgta gcagaccgtg
       61 gtcaacaaag atagatgtca attggtcacc gatcgctttt tgtaaaagaa ccccaacgac
      121 actagaatca acgccacctg aaagaccaag caagactttt ttgtcaccaa cttgttcacg
      181 gattttttct acttgcatat cgataaagtt atccattgtc cagttacctt cacagtgaca
      241 aacatcaaag gcaaagtggc gcagcaattc gattccgtat tcggaatgac gtacttctgc
      301 atggaattgg ataccataaa aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc
      361 tttgcttgtt ccgactgttt caaagccttc aggaacttta gttaccaagt caccatggct
      421 catccatact gtttgttgtt ttggtgtttc tttaaacaag acagcttgat cgcttgttac
      481 ttctaacaag gctttcccat attcgcggtt tttagcaggc tctacggaac cgccaagttt
      541 gtacgtgatc aattgcatac catagcaaat ccctaataca ggaataccta aattgaagat
      601 ctcaggatcg atcccaaatg cattctcatc atatacactg tttggtccgc cagaaagaat
      661 gattcccttt ggattcatcg cttttacttc ttctgcagta atgcggtggc tcaataattc
      721 ggaaaagact ccaagatcac gaatacgtct tgtgatcaat tggttatact gactaccgta
      781 gtctaatacg ataatctttt caacgttcgt catatcggca acgtttgtca aatttattac
      841 ccctatccct ttaaatttta tctcaaaata gtcaaactat tttaaaatgc taatattttc
      901 gcatgaaaat cgaatcgatg atatgatttt cagttttatg gagaattata tcggctcttc
      961 ctcgtgtagg caagatatat tcctgtaaat ttttcaaatt cacatttttc catacatctt
     1021 ttgccatttt aaaggcttct tcacgatctc cgatagcaaa acgataataa taattctctg
     1081 gattttggaa agccgtatcc agtaacgcac caaaacgatc caaataccat ttttcgatca
     1141 gcacgggatc ggcatcgaca tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct
     1201 gattcgcagg taattgaagc gtattgatcc cctcaacgat caagatatca ggctgctgga
     1261 tcaattcgta cttgtctgga atgatatcat acacatcatg tgagtaaaca ggtactttta
     1321 tctcttcctg accagatttt acacggttca agaaatcgat cagcatctcc atatcataac
     1381 tttcaggaaa tcctttacgg tccatgattc cacgctcttt taatatacga tttggataaa
     1441 gaaatccgtc agtcgtgatc agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg
     1501 tctgcagcag tctggctgtt gtactttttc caacggctac acttccggcg atcccgataa
     1561 taaaaggcgg tacttctacg tatttatgaa gaaataaacc tttactcaag aacaaagatt
     1621 caaattcttt catataaaga taaatcagat gagcaagagg tacatagata tctcgaacat
     1681 cctgcatgga aatgcggtca ttcaaacttt tgatattatc tagttcatct tgtgtcaaag
     1741 gtgcttgccc attacgataa aattctcgcc attcttctct tgccacacgg tagtagttca
     1801 ttggatcatt catattagcc tcctgcgttc tcccatcaat ggaactattt taacacaaaa
     1861 taaacgcaaa ccccaccact taagagagat tgccgatctt tttgtataat ctttataata
     1921 gaaagaaacg gagtcttatt cttgacttgc gcacggatac ataaaaggat agactgttgt
     1981 catgaaagaa atgagaagga tggaagacat gacaaatcat tactattctg aacgcccaga
     2041 tacggcgcac gattttgaac aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt
     2101 aacagattct ggtgtatttt cacgtgaaac agtggattat ggttcacgtg tcctgattga
     2161 tgctttcgat tgggaagagt tacctgatga aggaaagatc ttagatgtgg gatgcgggta
     2221 tggaccgatt gggttagctt tggcatttgc gacacagcgg tttgttgaaa tggtcgacat
     2281 taatgaacga gcggtaagtc tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt
     2341 tgatatccat caatcgaata tctatgaagc agttcatgaa gaaacctatg cagctatcgt
     2401 aagcaatccg ccgatccgtg ctggaaaaaa agttgtacat gaaattttga caggcgcata
     2461 tcctcggttg aagaagggcg gtacactgac aattgtgatc cagaagaaac aaggagcgcc
     2521 gagtgcgcaa aagaaaatgg aagaaacatt tggtaatgca gaaatcgtca caaaagataa
     2581 aggatattat atcatcaaaa gtgtgaaaga ataactagct gaaaaatagt ggagcgacga
     2641 aagagtggaa ctgggcgtgc atgtacgggt acgtatcagt tctttttttg ttgagaagtt
     2701 tttaaaaaca aagaactatt ttttcttcaa aaagaaatag taggaacaga gtcgttgttt
     2761 tttacgataa aataaataag atacagaact tgtatagaag gctgccaagt cggtagataa
     2821 aggaggtttt tatcaaaaaa catactttcc ttttttctct attcttttct tgggggtttt
     2881 ggtacactga tagggaattt agatagaagg gagccaatag gatgaagctg accatcagag
     2941 atatcgcaga aatggcaggc gtatcagtaa caactgtctc acagattttg aataacaaag
     3001 gaagcagatt tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt
     3061 ataagcccga ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga
     3121 tcgtccctga tgtcactgat ttcttcttct ccaaactgat cgaaggtgta gagagctatt
     3181 tgaatccttt gggctatgtg atcatgttat gcaattctcg tcatagccaa gaaaatgaga
     3241 tcaagtattt acaagagctt tcccaccgct cagtggacgg aatcttgctg gccacaccaa
     3301 atattttacc tgagaaatat gcattagaca gcggttttta tcaaaaaatg ccaatcattt
     3361 taatcgatcg cggattgaat cgtcgggata acggacggct tatcgtaaag gaatatgagg
     3421 gcgcctatca agctgtttcg ctgtttattg aaaatggcca tacgaagatc ggcatgctga
     3481 aggaaaccac tggatactat caattagaag aacgtttcaa cggatatcgt catgcgctga
     3541 aagatcatgg cctgcctttt aatgggaaat ttgttgaaca tggagagttg actgtccaag
     3601 gtggatatac tgcatcaaaa aaattactca aacataaaga tatcacggct attttttgcg
     3661 ggaatgatgc catggcgatc ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc
     3721 cagaagatat ctcagtcata ggattcgatg gattaaagct gtcagaatac atgattccgc
     3781 aattgacgac agtgcagcag ccaagttttg atattggatt ttatgctgca cgtttcctga
     3841 tcgatacgat cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa
     3901 ttattcgtga gagcgtaaaa gtattgacaa agggctaact cggcggtata ttgcttatgt
     3961 aagcatttac aaaggctagt tagtgctttt tttattttta gtaaaccagt ttactaaagt
     4021 ggtttacctt tcctaaaaat aatgtataat accgaatgaa gacaaggagg atcaggcaat
     4081 gcgaatggtg gatctgattg aaaaaaagag ggatggaaac gagctgtcga aagaggaaat
     4141 cgagtacatc gtcacgaact atacgaatgg aaagattcca gattatcaag tcagtgcatt
     4201 attgatggca attttttatc aagacatgac aaatgaagaa atcacgaact taacattggc
     4261 gattgctaat tctggtgatg tcatcgattt gagttctctt gaaggaataa aggtagataa
     4321 gcattcgaca ggtggcgttg gtgacacgac cacattgatt ttagcccccc tagtagctag
     4381 tgtcggagtg acagtggcta aaatgtctgg acgcggtcta ggttacacag gtggtacact
     4441 agacaagcta gaagcaattc ccggatttca gatcgagctt tcggatgaag aatttgtacg
     4501 cattgttaat gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg caccggcgga
     4561 caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt gattctcttc cgctgattgc
     4621 cagttcgatt atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt tggatgtgac
     4681 cactggagac ggtgctttca tgaagaacat cgaagatgca agacgtctgg caaagaccat
     4741 gacgagcatt gggaaactgg ctaaccgaga aacagtggcg gttatctcag atatgtcaga
     4801 acctcttggc gaagctatcg gaaatagtct agaagttgtc gaggcaatcg aaacattaca
     4861 aggaaatggt ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt
     4921 tcttgctggc aaagctaaga caaccgatga agcaagaacg ttgcttcaag aagctctgga
     4981 atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa aatcaagggg gagacccagc
     5041 gatcgtggaa catccagaac gaattttgac tgcacgctac acaatggagc ttcctgctaa
     5101 acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg caatgatgct
     5161 aggtgctggg cgtaaaacca aagaagaaga tattgatcac gctgttggat tgaaactgca
     5221 taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta ttgacgatct atagtaacga
     5281 cgaagaaatc acttctgtca ttgaattatt atataaaaat attgaaattg gtgaatcggc
     5341 aatgaagcca acgttgattc atgacatcat cacagaataa aggaaaaaag cggactttac
     5401 cgttttaaag aaaaggggaa aagaaatgga actaaaccga atgatcgacc atactatttt
     5461 gaaagcagac gcatctaaag aagctgtgat gcaaattatc gaagaagcaa aaaaatacca
     5521 tttttattct gtctgcatca atccagcatg ggttgcgcta gcgaaagaac aattacaagg
     5581 tgagccagta gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca cacccgagac
     5641 aaaagcattt gaaacaacag atgccatcaa taacggtgca gatgaagtcg atatggtcat
     5701 caatatcggt gctttgaaat caaaggatga tcaacaagtc caaaaagata tcgaagcagt
     5761 tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt attgaaacat cgttattaga
     5821 tagagaagaa atcatccgag cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa
     5881 aacatcaact ggtttctcaa caggcggagc aaaggtagag gatgtccgtt tgatgcgtga
     5941 aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt atccataacg aagaagaagc
     6001 aatggcaatg atcgaagctg gtgctacaag aatcggaaca agcgcaggag tagcaatcgt
     6061 atctggttca actggtgaag gctattaatt ttttcaaagg agaagctcat ggcagaactc
     6121 aaacaagaat ggatcaacac agctattgaa gcgttagata aagcctatgt tccttattcc
     6181 cattttccgg taggcgcttg tctcgtaacg gaatcaggca agatttatca aggcatcaac
     6241 attgagaatg catcatttgg attaacgaat tgtgccgagc gcacagcttt tttcaaagca
     6301 gtatctgaag gagaaaaaac atttacgcat ctagtcgtag cggggcacac ccccgatcct
     6361 atctctcctt gtggtgcatg tcgacaagta atggcagaat tctgttcccc tgatatgccg
     6421 gttacgctag taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct
     6481 tatgcattta ctgaaaaaga tctataactc atgttcattc agtcagctga ctgttgaaaa
     6541 ataaaacaat tattttagga ggctttacag aaatgaaaaa agcaaaatta tttggtttag
     6601 gtgctgtcgc actagcagca gggttatttt taggtgcatg cggcaacaac ggctctactg
     6661 attcatccgg tggcaaaagc tcatcagaca caacaactgc agcattgatc acagacacag
     6721 gtggtgtaga cgaccgttca ttcaaccagt cagcttggga aggactagaa aaatggggga
     6781 aagatcaagg actttcacga ggaaatgatg gcttccaata tttccaatct tcaaatgaat
     6841 cagattacat cccaaatatt gaccaagcat tgaatgctgg attcaaaacg atttttggta
     6901 ttggttataa actaaaacca gcgatccaag aacaagcaac aaacaataca ggtacgaact
     6961 ttgttattat cgatgatgtc atcgatggat tagataacgt tgtatctgca actttcaaag
     7021 acaacgaagc ttcttatctt gcaggtgttg cagcagctta tacgactgaa acaaacgtag
     7081 tcggctttat cggcggtgta aaaggtgaag taatcgatcg ttttgatgct ggattcaaag
     7141 ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat caaagtattg aatcaatatg
     7201 ctggtgactt ctctgctcca gataaaggac gttcaattgc tcaaggtatg tatgcacaaa
     7261 acgcagatat catcttccat gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa
     7321 aatcattgaa cgaatcaggc gacaaaaaag tttgggttat cggtgttgac cgcgaccaat
     7381 cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg acatctacac
     7441 tgaaagcagt tggaacagta gtagaagatt tggcacaaaa atcagcggac ggtaaattcc
     7501 ctggcggcga acacactgtt tatggattga aagaagacgg tgttggtcta acagaaggac
     7561 aactttctga cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg
     7621 acgtaaaagt tccagaaact cctgaagaaa actaaaacca tttctatagc cttgttagac
     7681 acatgtttga caaggctatg gtttgattag aacaagaatt gttcatttat ccaagctgcc
     7741 ttgtgacagt ttggataagg gaacaactcc ctgaagtaat gtttgtttca aaagaagaac
     7801 gctcactaag cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa
     7861 aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga atattaccaa agaatttgga
     7921 acattcaagg caaatgacaa catcaattta caagtaaagg caggagaaat tcatgcgttg
     7981 cttggagaaa acggtgctgg caaatctaca ttgatgaacg tgctttccgg attattagag
     8041 ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa
     8101 gccaaccaat tagggattgg gatggtccat cagcacttta tgcttgttga tgcctttact
     8161 gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg cagggatgct tgaccataaa
     8221 aaagcacgaa aagagatcca aaaagtttct gaacagtatg gattgtcagt caacccagat
     8281 gcttacgttc gtgatatttc agttggaatg gaacaacggg tagaaatttt aaaaacactt
     8341 taccgaggag cagatgtact gatttttgat gagccgacag ctgtattgac ccctcaagaa
     8401 attgatgaat taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc gatcattttg
     8461 attacgcata agttagatga aatcaaagca gtagctgacc gctgtacagt tatccgccgt
     8521 ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct cacaacaatt agccgatatg
     8581 atggttggaa gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc
     8641 gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac
     8701 ttgaacttag aggttcgtgc tggcgaagta cttggtatcg ctggaatcga tggaaacggg
     8761 cagtcggagt tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg acatatcaag
     8821 ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga
     8881 cacgtgccag aagaccgtca taaatacggg ttagtcctag atatgacatt atctgaaaat
     8941 atcgcactac aaacctatca tcaaaaacct tacagtaaaa acggcatgct gaattattca
     9001 gtaatgaatg aacatgccag agaattgatc gaagaatatg atgttcgaac aacgaatgaa
     9061 cttgttcccg caaaagcttt atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa
     9121 gtcgaccgag atcctgatct gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt
     9181 gcgatcgaat ttattcataa acgtctgatc gaacaaaggg acaaatacaa agcagtgtta
     9241 ctgatcagtt tcgaattaga agaaatttta aatgtttcgg atcgtattgc tgttatccat
     9301 gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga attaggtcta
     9361 ttgatggcgg gctatacctt agaagaagca cgaaaagagt tagaaaaggg ggctggcgca
     9421 catgaatgat cgcagcgaaa aattccgtaa tttattagtg cctgtacttt cagttgtttt
     9481 aggattgatc ttaggcgcga tattgatggc tgcttttggt tttcagccca tccaaggtta
     9541 ttcttctatg ctagatgcag cccttggttc gcaaagaggg ataggtgaaa cgttaaggca
     9601 agcaacgccg ttgattttta cagcattagg tttttctgta gctaactcag ctgggttttt
     9661 caatattggg ctttctggac aagcactttg cgggtggatc gtcagtgtct gggtggcact
     9721 tgcgtttcca gatctgccaa aagttgtttt gcttccgtta tgcgtgatct taggtgcctt
     9781 agctggtgcg atggcagcgg ctgttccagg tttacttcga gcattttttg gaacaagtga
     9841 agtgatcgta acaatcatga tgaactatat cttattatac gtaagcacat ttgctttaca
     9901 tgaagtgatg cctgaaagct atcgctcaag tttggactct tccaatatga tcagtcaaaa
     9961 tgcctcactg cggttaccat ggttaacaca agcttttggc ggctctcgcg taaactcagg
    10021 attattttta gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga aaacgacttt
    10081 aggatttgaa atccgttctg ttggattgaa tccttttgct tctgagtatg caggaatgag
    10141 tagtaaacgt acgatcgttt tgtcgatggt catttctggc gctttggcag ggcttggcgg
    10201 tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt caaacgactt cgctatctat
    10261 tggatttgac ggaatggctg tttccttgct tggttctgga tcagcaatcg gtattctttt
    10321 atcagcgatc ttgttcagta ttttgaagat cggtggactt ggcatgcaga caggagcagg
    10381 tgttccgttt gagatcgtca atgtatcgat tgctttgatt atcttctttg tcgggatcaa
    10441 cttcctgatt cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag aagagatcgt
    10501 tgcgacaata gaatcgaaac caacgaatca aaaccatgaa gggggcgaac tataatgtcg
    10561 actatcgaat taattgcctt gatccttact tctacactgg tttattcaac accgctaatt
    10621 ctaacgtcac taggcggaac tttttctgaa cgaagcggga tcgtaaacgt agggctagag
    10681 ggaatcatgg tcatgggtgc ttttagttcc atcgttttca acttgacgat gagtccatca
    10741 cttggttcta tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt
    10801 tctctgattc acgcagtagc aacagtcagt ttgcgtgctg accatattat cagcgggaca
    10861 gtaattaatc tgatggcacc tgctttaggt gttttcttga tcaaagcatg gtatggtaaa
    10921 ggtcaaactg ataacatcac gcagaatctg gggtattttt ctttcccagg attagcagat
    10981 attccggtca ttggaccaat tttctttaaa aatacattct tacctgctta ttgtgctatt
    11041 ttgattgctg tcctagcttg ggtgatcatc ttcaagacga aattcggctt gcgcctccgt
    11101 tcagttggtg aaaatcctca agctgccgac acattaggta tcaatgtata tgctatgcgt
    11161 tattcaggcg tactgatttc tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag
    11221 tcgatttccg gtaacttttc agctggtacg atcgttggac aagggttcat ctccatggct
    11281 gctatgatct ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt gttctttggt
    11341 tttgctcaaa gcttgagtat cattggcgca caattaccag ttatttcttc gattccgcca
    11401 gtattgctgc aaattgctcc atatttgctg acaatcattg tgctggttgt tttcttaggg
    11461 aaagcttctg gtccaaaagc aaacgggaag aactatatca aatcaaaata atcttttagc
    11521 gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca
    11581 ataaaaataa aaagaaagaa ggaataatca tgtttaaacg cgtacatctg atcgtcatgg
    11641 attcagtagg aattggagaa gcaccagatg ccgataagtt cggtgatgtt ggttctgata
    11701 cattaggaca cattgcgaaa gaagcaggat tgaacgttcc acatttagag cagcttggtt
    11761 taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga
    11821 cgaaattaga agaagtttct gtagggaaag atacgatgac aggccactgg gaaatcatgg
    11881 gattgaatat caaaactcca tttcgtgtgt ttcctaatgg attccctgat gatctgatca
    11941 aacaaatcga agaattttct ggccgtaaag tcgtatgtaa caaaccttat agcggaacag
    12001 ctgttattga cgattacggt gaacaccaaa tgaaaacagg cgatttaatt gtatatactt
    12061 ctgccgatcc tgtccttcag atcgcggcac atgaagaaat cattccttta gaagaattat
    12121 atcggatttg ccaattcgtt cgtgatataa caaaagatga tccgtatatg attggccgaa
    12181 tcattgcacg tccgtacgtg ggtgaaccag gagacttcac acgaacaagc aatcgccatg
    12241 attatgcgtt gaatccattc ggacacacag tccttgattc tttgaaagaa gcaggaaagg
    12301 acgtgattgc tgttggaaag atcaacgata ttttcaacgg acaaggaatc actgaatctg
    12361 tccgcacgaa aagcaatatg gatggcgttg accagctgct gaaagttatg aaaaacgatt
    12421 tttcaggaat cagctttacc aatttagtag attttgatgc attatacggt catcgtcggg
    12481 atgtagtcgg ctatgcgcat gcgattgaag aatttgactt acgaattcca gaattgcttg
    12541 aagcgatgga agaagacgac ttgctaatga ttacagccga tcacggaaac gatccgacgt
    12601 ttactggtac cgatcatacg cgagaatatg taccgttatt ggtttacagt aaaaaaatga
    12661 aagaacaagg ggcattgcct caaggatatt attcagatat ttcagcaacg attgctgaga
    12721 actttagtgt ttcttcgact gaaaatggcg aaagcttctt gaaattatta aaataatctt
    12781 tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt
    12841 tatccttgat aaaggagtaa aagaaattga ttttggtttg attctaggtt caggattagg
    12901 tgaattagca gatgaaatcg aagatgctgt aatgatttct tatgatgaga tcccgttctt
    12961 tcctgtttcg acagtagttg ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa
    13021 gaaagtgtta gccatgcaag ggagattcca ctactatgaa ggtcattcca tgcaaacagt
    13081 gacctatcca gtacgtgtga tggccggact aggggcccat tcagtcattg tgactaatgc
    13141 atgtggtggt gtcaacgaat catttgtacc aggagatttg atgctgatta ccgatcatat
    13201 caattttact ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt
    13261 tccagatatg agtcaagctt atacgcctga ctatcaagca acagcaaaag cagcagcaga
    13321 aaaactaggt ttggatctta aagaaggcgt atatatgggc tattctggac caacttatga
    13381 aacgcctgcg gagatccgaa tgtctcggac aataggagcc gatgcagtag ggatgtcgac
    13441 tgttccagaa gtcatcgtag cagctcacag cggattaaaa gtgttaggaa tctcttgtat
    13501 taccaactta gcagcaggta tgcaagcgaa tctgagccat gaagaagttg tagaaacaac
    13561 tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa acattgacat tattgtaata
    13621 ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga aggcgagatc gccgataaga
    13681 ttttacttcc gggagatcct ttgcgagcta aatatattgc ggagactttt ttggaagatc
    13741 cagtttgtta caatcaggta cgcggtatgt taggctttac cggcaaatat aaaggagaac
    13801 gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc caccatctat gcccatgaat
    13861 tgatccagtc gtatggtgta aagaaattga ttcgagtagg tacatgcggt gcattgtcaa
    13921 aagacgtcca tgttcgtgat ttagtactag cacaaggtgc agcgaccagt tcttcgatga
    13981 tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag tgatttcaat ctattactga
    14041 aagcatatga gatagctaaa gaaaagggct atacggttca tgtaggaaat gttctttcgg
    14101 aagattcttt ctacaaagat gatttgacag agacgttcca attagctgaa cttggcgttt
    14161 taggagtgga aatggaagca gcggctcttt attacttagg cgcgaaatac catgtgcaga
    14221 cattaagttt gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca acagctgcag
    14281 aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg atagcagaat
    14341 aagaaggaaa tgaaaaagct gccctgcatt aagtaataag aacatccaag caaaaatagc
    14401 tcctcatgtt tttgacttgg ctggacttat tattgcaaat gtaagctttt gaacaccgtt
    14461 taccaagagg ttgtgaatct gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac
    14521 agcttttcat gtttgatgaa tattgtgact tgtgaccgag aagttggcag gagaaaaccg
    14581 tttatacgaa aaaagggact gtgacaaatc gtgtcacagt ccctttttta tagtgttttt
    14641 tataggtagt attttttagc aactgttaag tcatcattta attcataaac aagtggttga
    14701 cctgttggaa tttcaagatc catgatgtct tcgtcagaga taccttcgat atgtttagct
    14761 agggcgcgta atgagttacc atgtgcagct actaaaacag ttttgttgtc tagtaatgct
    14821 ggagcaattt catcttgcca gaatggcaaa gcgcgttcta atgtcacttt caagttttct
    14881 ccaccaggaa tatcgcgttt gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct
    14941 tcgtcagttt cttccattaa tggaggaaga gtatcgtaag aacgacgcca gatgtgcact
    15001 tgttcgtcgc cgtatttttc agctgtttct tttttgttca aaccttgtaa tttaccatag
    15061 tgacgttcgt ttaagcgcca tgatttgatt tgtggtaccc aaagttgatc tgaatattct
    15121 aagatcaagt tacatgtttt gattgcacga gttaaaacag aagtgtaagc aacatcaaat
    15181 tcgattccag cttctttgat tttgcgtccg ccttcttttg cttcttcgat tccttctggt
    15241 gctagattta catcagccca accagtaaat tggttcaatg cattccattc actaagaccg
    15301 tgacgagaaa aaactaattt tggcatgttt agctctcctt ttaagatagg tttatatttc
    15361 tgaaattttc agttctgttt tattgtacaa ttttttggaa gagatttcca tcgaaaaatt
    15421 agataagatt ctatgagaaa acaagtagag aatataaaaa aacgaacagt gcccacgcaa
    15481 ctgttcgttt ttaggtatta gatgcataat tgctcgattt ctttttcagg taaattgata
    15541 tatttataac atgttccaac tgaaacaccg cattttgaag aaataaattg gatcgtttct
    15601 tttttttcat aatagagacg tcggattttt ttgatcgttt ttgcatcgat ttttggtctt
    15661 ccgccgattt ttcctttttt acgagcttca tctaatccaa ctaatgtacg ttctttgatc
    15721 aatgcgcact ccatgtttgc taggttttcc attaattgga aatagatttt tcccatttcg
    15781 cgcgtatcga ttccttcatc aagactaatc aagtgcagcc cgcgatcttt gaataattgc
    15841 gtgagttctg ttaattgccg cgtagatttg cctaatcgat gaagttggca aatgactagt
    15901 gcatcgcctt gttgcatgtc agcgatcact gactccaaat gaacagcagt agaatcattt
    15961 tgttccactt catatgtctc atggaaaatt tcttgacaac caaagtcaga taattgatca
    16021 acctgagttg ttaaatcaac gtcatttatg gttgtgcgtg cataaccaat aattttcatg
    16081 gtgattcctt tctagcagtg attttctaac tggatataac agtaaaaatg tagcataaag
    16141 tacgtcaaaa agatagtttt ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa
    16201 gcagtttttc tttatttacg acggattgcg tcaagaatct caacgccttt ttctgagccg
    16261 acaactactt tagaaaccat gtttaggaaa agcccatgtt ctactacccc aacaagttca
    16321 tctaaatagg tagctaagct ttctggctgt tcgatgactt ctagatgcag atcaatgatg
    16381 taatgaccac catctgtggt taagacttct ccgttttcgt ctgtacgcaa gacgggttgg
    16441 aatctttttt cttcaaaaat atgaagtagc tgttgggagc cataagggat cacttcaact
    16501 gggagtggga acttcccaag tttatcgact aatttagaag aatcaacgat ccaaatcgtt
    16561 tcttttgagt acgtagcgac tattttctcg aaaagcaaag cagcgccgcc gcctttgatc
    16621 ccttgaaaat cagcactgat ttcatcagca ccgtctattg tcaaatcgac tacggggact
    16681 tcatcaatac tctttaacgg tattcctaat ttttccgctt gttcttttgt cgcatttgaa
    16741 gtagtaactc ctgtgatacg taaaccttct tcacgcatac ggcgaccaat ctcttctacc
    16801 atataatagg cggttgaacc ggtcccgaga cctacgatca tcccgtcctt tacgaatttc
    16861 gcggcttcaa tgccagctaa ttgttttaaa ttcatctttg cgtccacctt tttttagttt
    16921 gagcatcgtg ctttcctcac tattttagcg tgatttacgg taaaaagata ccattctttt
    16981 ttattccaat aaagcttttt caaaaaaaat tgcatctctt gttgacatgt tttttcattc
    17041 gtgatattct aacaaaggtg ctttatctac aggtttctgt gaagaaacgt gctgactgtt
    17101 tatcaaagca tatttgataa ggatgcagac attgcattta ttttttatca caaaaaaaga
    17161 accacctgga tgtgtggaac tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta
    17221 caattaacca attagtacgt aaacctcgta aatcaaaggt ggaaaaatct aattcacctg
    17281 ctttgaacaa aggatataac agctttaaga aaactcaaac taacgtaaac tcacctcaaa
    17341 aacgtggggt agcaactcgt gtgggtacaa tgacacctaa aaaaccgaac tcagctttac
    17401 gtaaatatgc acgtgttcgt ttgtcaaact taattgaagt tactgcttat atcccaggga
    17461 ttggtcataa cttacaagaa cacagtgttg tattattacg cggtggtcgt gtaaaagact
    17521 taccaggggt acgttaccat atcgttcgcg gtgctttaga tactgctggt gttaacgacc
    17581 gtaaacaaag ccgttctaaa tacggaacta aaaaacctaa agcttaattt taattattaa
    17641 acaaatgaaa cttgcttcta ctatatagaa gtaattacta tgaacaatat ttcggaagga
    17701 ggagttacgg atgccacgta aaggtcctgt tgcaaaacgt gatgttttac cagatcctat
    17761 ttataactca aaattagtaa ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg
    17821 gattgcagct aacattatct ataatgcctt tgacttgatc aaagaatcta caggtaacga
    17881 tccattagaa gtgtttgaac aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc
    17941 acgtcgtgtt gggggttcaa actaccaagt accagttgaa gttcgccctg aacgccgcgt
    18001 tactctaggt cttcgttggg tagtaaacta cgctcgcttg cgcggtgaac acacaatgga
    18061 agaacgctta gcaaaagaaa tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa
    18121 acgtgaagac actcataaaa tggctgatgc caaccgtgcg tttgctcact atcgctggta
    18181 agattctctc tgtttggtcg gcttttataa gtcgacctca gatagtgtcc ttatttttag
    18241 caactagata gtaaagagag gagaaacaag gagaatgacc agagaatttt ctttacaaaa
    18301 cactcgtaat atcggtatca tggcccacat cgatgctggt aaaacgactg ctactgaacg
    18361 tatcttgtac tatactggta aaatccataa aattggtgaa actcatgaag gagcttcaca
    18421 aatggactgg atggaacaag aacaagaacg tggtattacg atcacttctg ctgctactac
    18481 agcacaatgg aagggtcacc gaatcaacat catcgacaca ccaggacacg tagacttcac
    18541 agttgaagtt gaacgttcat tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca
    18601 atcaggtgtt gaacctcaaa ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc
    18661 tcgtatcgta tttgcaaaca aaatggacaa gatcggtgct gacttcttat attcagtatc
    18721 aactttacat gaccgtttgc aagcaaacgc tcacccaatc caattaccaa ttggtgcgga
    18781 agacgacttc acaggaatta tcgaccttgt aacaatgaag gctgaaatgt atacaaacga
    18841 tcttggaaca gagatcgaag aaacagaaat tcctgaagaa taccgcgaat tagctgaaga
    18901 atggcgcgaa aaattggttg aagctgtagc tgaaactgat gaagaactta ctttgaaata
    18961 cttagaaggt gaagaaatca ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt
    19021 taacgtagaa ttctacccag tactatgtgg ttcagcattt aagaacaaag gggttcaatt
    19081 actattggac gctgttcttg attacttgcc atcaccgtta gatatcccag ctatcaaagg
    19141 gattgaccct aaaactgatg aagaagtaga acgtcctgca gacgacagcg ctcctttctc
    19201 agctttagca tttaaagtta tgactgaccc attcgtaggt cgtttaactt tcttccgtgt
    19261 gtactctggt gttttacaat caggttcata cgtacaaaac gcaacaaaag gcaaacgtga
    19321 acgtgttggt cgtatcctac agatgcatgc caactctcgt tcagaaatct ctgaagttta
    19381 cgctggagat atcgctgctg ctgttggttt gaaagacaca actactgggg acacactatg
    19441 tgatgaaaag agtctagtta ttcttgaatc catggaattc ccagaaccag ttatccaagt
    19501 cgctattgaa cctaaatcaa aagctgacca agataaaatg ggtgtggctt tacaaaaact
    19561 atctgaagaa gatcctactt tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat
    19621 cgctggtatg ggtgaacttc acttagatat catcgttgac cgtatgcgtc gtgaattcaa
    19681 agttgaagct aacgtaggtg cgcctcaagt atcttaccgt gaaactttcc gtgctggcac
    19741 tcaagccgaa ggtaagtttg tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg
    19801 gattgaattt acaccaaatg aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg
    19861 tggggttgtt cctcgtgaat acattccagc tgttgaaact ggtttgaaag atgctatgga
    19921 aaatggtgtc ttagctggct atccattagt agatatcaaa gctaaacttt acgatggttc
    19981 ataccatgat gtcgattcaa atgaaactgc cttccgtgtt gctgcttcta tggcattacg
    20041 tgcagcagct aagaaagcta atccagtaat ccttgaacca atcatggctg tagaagttgt
    20101 tatccctgaa gattatttag gtgatgttat gggtcacgta actgctcgtc gtggacgtgt
    20161 tgaaggtatg gaagctcgcg ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc
    20221 tgaaatgttt ggttatgcta caactcttcg ttctgctact caaggtcgtg gtacatttac
    20281 aatgacattt gaccactacg aagatgtacc aaaatcagta caagaagaaa tcattaagaa
    20341 aaacggcgga aaagctgaat aatttcttaa aaccggatag tcctgcaaag agattatcca
    20401 aagagactat atttttaata tagtttcgtg taaaataatt atcgatgata tgggcatgtg
    20461 gtgtaccttg tgtacatcat atacctatca gacaaataaa aaatatattg tatttttagg
    20521 aggacatttt aaaatggcaa aagaaaaatt tgaccgttct aaaccacacg ttaacattgg
    20581 tactatcgga cacgttgacc atggtaaaac tacattaaca gctgcaatca caactgtact
    20641 atctaagaaa aacggcggcc aagctatggc ttacgatcaa atcgatggtg ctccagaaga
    20701 acgcgaacgc ggaatcacaa tctcaacagc tcacgttgag tatgaaacag atacacgcca
    20761 ctacgctcac gttgactgcc caggacacgc ggattacgtt aaaaacatga tcactggtgc
    20821 tgctcaaatg gacggagcta tcttggtagt ttctgctgct gacggcccaa tgcctcaaac
    20881 tcgtgaacac atcctattgt ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa
    20941 caaagtagac atggttgatg acgaagaatt actagaatta gttgaaatgg aagttcgtga
    21001 cctattaaca gaatacgaat tccctggtga cgatgttcct gtagttgctg gatcagcttt
    21061 gaaagctcta gaaggcgacg cttcatacga agaaaaaatt cttgaattaa tggctgcagt
    21121 tgacgaatac atcccaactc cagaacgtga caacgacaaa ccattcatga tgccagttga
    21181 agacgtgttc tcaattactg gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca
    21241 agttcgcgtt ggtgacgaag ttgaagttgt tggtattgct gaagaaactt caaaaacaac
    21301 agttactggt gttgaaatgt tccgtaaatt gttagactac gctgaagctg gagacaacat
    21361 tggtgcttta ctacgtggtg ttgcacgtga agacatccaa cgtggacaag ttttagctaa
    21421 accaggtaca atcacacctc atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga
    21481 agaaggtgga cgtcatactc cattcttcac taactaccgt ccacaattct acttccgtac
    21541 aactgacgta acaggtgttg ttgaattacc agaaggaact gaaatggtta tgcctggcga
    21601 caacgtaaca atggaagttg aattaatcca cccaatcgct atcgaaaacg gtactaaatt
    21661 ctcaatccgt gaaggcggac gtacagttgg tgccggtgtc gttacagaaa tcaaagctta
    21721 attcacttaa gttttaaagt aaagcaacat ttattcggac gtaatcgctc ggaacgaagg
    21781 tcttaggatc tttgttccga gcttttttat ttattgcagg atcaggaaaa aagacaggag
    21841 tattataaat agaagaaaca cgaaataaaa gagtcagtat tttctaaatg aaactttatg
    21901 aaaacaaaat gttttttaca agaaaaagcc ttgctttgtg agtttatata tattataatg
    21961 acaaaggtgc gtttacttta gtgaattgca cagacgtaat cagaatgatt tcgtcggcta
    22021 agaaacgaga ggttgcgaca cgcccggacg ctttgccacg aacgagcgtg acggaaattt
    22081 tcgtggagct atgtctactt ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa
    22141 aaattcgtat ccgtttaaaa gcgtatgaac accgtatttt agatcaatca gcggataaaa
    22201 tcgtggaaac tgcaaaaaga actggagcta gcgtgtctgg tccaattcca ttaccaactg
    22261 aacgcagtct ttacacaatc atccgtgcga ctcataaata caaagattca cgcgaacaat
    22321 tcgaaatgcg tacacacaaa cgtcttatcg acattgtgaa cccaacacca aaaactgttg
    22381 atgctttgat gaagctagac ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact
    22441 aaaacaaaaa taatggaggt gtactcatga ccaaaggaat cttagggaaa aaagtgggaa
    22501 tgacacaaat cttcactgaa tctggtgaat taattccagt aactgttgta gaagctactc
    22561 caaacgtagt actacaagtg aaaacaatgg aaacggatgg ctacgaagct attcaagttg
    22621 gttaccaaga tatgcgtgaa gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag
    22681 caaacacggc tcctaagcgc ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg
    22741 aagtaggaaa agaaattaca gtagatgttt tccaagcagg agacattatt gatgtaacag
    22801 gtactacgaa aggtaaagga ttccaagggg ttatcaaacg ccacggacaa agccgcggac
    22861 caatggctca cggttctcgt taccatcgtc gtcctgggtc aatgggtcca gttgcaccta
    22921 accgtgtatt taaaaacaaa cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc
    22981 aaaacttgga agttgtacgt gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg
    23041 ttcctggagc taaaaaatct ttaattacta tcaaatcagc tgtgaaagct aaataattgt
    23101 aggaagaaag gaggaactaa ggaatgccga atgtagcatt attcaaacaa gatggaagcc
    23161 aaaacggtga aatcacactt aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg
    23221 tttacgatgc aatcgtaatg caacgtgcgt cattaagaca aggaacacac gcggttaaaa
    23281 accgtagcgc tgttcgcggc ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg
    23341 cacgtcaagg atctatccgc tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa
    23401 caccacgttc ttacagctac aaacttccta aaaaagttcg tcgtttagca atgaaatctg
    23461 tattgtcaga aaaagttgct gaaaacaact tggtagcaat cgaaggcttg aacttcgacg
    23521 caccaaaaac aaaagaattc aaacaagttc ttgctaactt atctatcgac tctaaagtat
    23581 tggttgttct agaaaaaggt aacgactttg ctgctttatc agcacgtaac ttaccaaacg
    23641 tttctgtagt aacttctgat aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt
    23701 tagcaacaca aactgctctt actcaaattg aggaggtgct tgcataatga acttactaga
    23761 cgtaatcaaa cgcccagtga tcactgaaaa atcaatgctt gccatggatg acaagaaata
    23821 cacttttgaa gtggacactc gcgcaaacaa aactttagta aaacaagcgg tagaagctgc
    23881 ttttgacgtt aaagtcaaaa acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat
    23941 gggcaaatat gcaggatata caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga
    24001 ttcaaaagaa atccaattat tcgaagctgc tgaataagca ctaatagata actcattaaa
    24061 ttaggaggga aaaacgtggc gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac
    24121 atgacaggtt ctgattttgc tgaaatcaca acatcaacac ctgaaaaatc attgttacaa
    24181 ccattgaaaa accatgctgg acgtaacaac aacggccgca tcactgtacg tcatcaaggt
    24241 ggcggacaca aacgccaata tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt
    24301 gcaactgttc aaacgatcga atatgatcca aaccgttcag ctaacatcgc gttagtacac
    24361 tatgaagatg gagtgaaagc ttacatctta gcacctaaag gactagaagt gggtatgaaa
    24421 cttgtttcag gtccagaagc agatatcaaa gtcggaaatg ctcttccatt agaaaacatt
    24481 ccagttggta ctgttatcca caacattgaa atgaaacctg gtaaaggtgg acaattgatc
    24541 cgttctgctg gtacaagtgc tcaagtactt ggtaaagaag gcaaatacgt attgatccgc
    24601 ttgaactctg gtgaagttcg tatgatctta gctgcatgcc gtgctacaat cggttctgta
    24661 ggtaacgaac aacacgaatt aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt
    24721 aaacgcccaa ctgtacgtgg tagcgtaatg aaccctaatg atcacccaca cggtggtggt
    24781 gaaggtaaag ctcctatcgg acgtaaagca ccagtttctc cttggggtca accagctctt
    24841 ggttacaaaa ctcgtaacaa aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa
    24901 aataaataat tactttgacc atgtgtcgca cattttgaga ggaggttcac catgggtcgt
    24961 agtttaaaga aaggaccttt tgtcgatgag catttaatga aaaaagttga agctcaagca
    25021 ggggttgaaa agaaaaaagt tatcaaaact tggtcacgcc gttcaacagt tttcccaagt
    25081 tttgttggat ataccatcgc agtttatgat ggacgtaaac atgtaccagt ttacatccaa
    25141 gaagacatgg taggacacaa attaggtgaa tttgcaccta caagaactta tcgtggacat
    25201 gctgctgacg ataagaaaac gaaacgctaa tttgagggga ggataaatcc aaatggcaga
    25261 acaaatcaca tcagctaaag caactgcaaa aacagttcgc acttcacctc gaaaagcccg
    25321 tttagtaatt gaccttatca gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt
    25381 cacaccaaac aaatctgctg gaatcattga aaaagtattg atgtcagcag ttgcgaatgc
    25441 agaaaataac tttgacttag atgttgaaaa cttggtagta agcgaagctt ttgttaacga
    25501 aggacctaca atgaaacgtt tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa
    25561 acgtacaagt catatcacgg tagtcgtatc agaaaaataa ggagggataa tcagtgggtc
    25621 aaaaagtaca tccaattgga atgcgtgtag gcatcatccg cgactgggat gctaaatggt
    25681 atgctgaaaa agagtatgct gagttcttac acgaagattt aagaatccgt aaatttattt
    25741 caactaaact tgctgatgct gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg
    25801 taaacatttc aattcacaca gctaaaccag gtatggttat tggtaaaggc ggttctgaag
    25861 tcgaaagcct aagaaaagaa ttaaacaaat taactggtaa aagaatccat atcaatatcg
    25921 tggaaatcaa aaaaccagat ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat
    25981 tagaaaaccg tgttgctttc cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg
    26041 ctggcgctca agggatcaaa actcaagtat ctggtcgtct aaacggagca gatatcgctc
    26101 gttcagaagg atactcagaa ggaactgttc ctcttcatac tttacgtgcg gacattgatt
    26161 acgcatggga agaagcagat acaacatacg gaaaactagg agttaaagtg tggatctatc
    26221 gtggagaaat tcttccaact aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt
    26281 acctaaacgt gtaaaacacc gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg
    26341 cggaaaagaa gtagcattcg gtgaatacgg tttgcaagct gttgattcac attggatcac
    26401 aaaccgccaa atcgaagctg ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa
    26461 agtatggatt aaaattttcc ctcacaaatc ttatactgcc aaagcaattg gggtacgtat
    26521 gggttctggt aaaggggcac ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat
    26581 gtttgaaatc gcaggcgttc ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca
    26641 caaattacca atgaaaacta agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga
    26701 aggttaaaga aatcagagaa ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa
    26761 aagaagaatt gtttaatctt agattccaat tagcaacagg tcaattagaa aacactgcac
    26821 gtattaaaga agtacgtaaa tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta
    26881 agtaattatg gaaggaggcc attaagcgta tgactgaagg aagaaatcaa cgcaaagttt
    26941 atcaaggtcg cgtggtttca gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa
    27001 agaaaaacca ccctatctat ggcaaacgca tgaactattc taagaaatac aaagtgcatg
    27061 atgaaaacaa cacagcaaaa gttggcgaca tcgtgagaat tatggaaact cgtccattat
    27121 cagctacaaa acgtttccgt ttgttggaag tagtcgaaga agcagttatt atctaataac
    27181 acgagatttt cctatataga ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc
    27241 aagcagaaag tcgtttaaaa gtcgctgata actcaggcgc acgtgaaatt ttgacgatca
    27301 aagtccttgg tggatctggt cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg
    27361 ttaaacaagc aacgccaggt ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg
    27421 ttcgaacaaa atcaggagct cgtcgtacag acggttcata tattaaattt gatgaaaatg
    27481 ctgcagtgat tattcgtgat gataaaagcc ctcgtggaac acgtatcttt gggccagttg
    27541 cacgtgaact acgtgaaaac aacttcatga agatcgtttc actagcacca gaagttttat
    27601 aatcttgaga cacttatcaa aggaggtgcg aaacagttat gtttgttaaa aaaggcgata
    27661 aagttaaagt tatcactgga aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc
    27721 cgaaaaaaga taaagttatt gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt
    27781 ctcaagctgc tcctcaaggt ggaattgttg agatggaagc gccaattcat gtttctaacg
    27841 tgatggtggt tgactctact ggtgtagctg gccgagttgg ctacaaagaa gtagacggca
    27901 aaaaagtccg tgtttctaaa aaaaccggtg aagttttaga taaataaaag tttaaggaag
    27961 gagggtctta ctgaatgaac cgcctaaaag aaaaatatct taaagaagta actccatcat
    28021 tgatggaaaa atttaactat agctctgtta tgcaaacacc taaagttgaa aaaatcgtca
    28081 tcaacatggg tgttggtgat gcggtatcaa acgctaaaaa cttagataaa gcagttgaag
    28141 aattaacttt gatcactggt caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg
    28201 gattccgttt acgtgaagga atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa
    28261 tgtacgaatt cttagacaaa ttggtttctg tttcactacc acgtgtacgt gacttccatg
    28321 gtgtaagtaa aaaagccttt gacggtcgtg gaaactatac tttaggtatt aaagaacaat
    28381 tgatcttccc agaagtagat tatgatttag tagataaagt acgtggtatg gacatcgtta
    28441 ttgtaacaac agcaaactct gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc
    28501 cattccaaaa ataattaaag gaggcgaact acgtggctaa aaaatcaatg attgctaaaa
    28561 acaaacgtcc tgcaaaattt tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc
    28621 cacattcagt ttatcgtaag ttccatcttt gccgtatttg cttccgcgaa cttgcctata
    28681 aaggtcaaat tcccggcgtg aagaaagcta gctggtaaaa agtaaattcg cataagaagg
    28741 aggtaaaaga tcaatggtca tgacagatcc aattgcagat tttctaactc gtatccgtaa
    28801 cgcaaacatg gttaaacatg agtctttaga agtacctgca tcaaaaatta aacgtgacat
    28861 cgctgaaatc ttgaaacgtg aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa
    28921 acaaggcgtg atccgtgttt tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa
    28981 cttaaaacgt atctctaaac caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa
    29041 agtattgaac ggtttaggta tcgcaatcat ttcgacttct gaaggtgttg tcactgataa
    29101 agaagctcgt gaaaaaaata tcggcggcga agttatcgct tacgtatggt aatcaagtag
    29161 aatctacaag gaggtgtctt taagtgagcc gtatcggaaa taaagtcgtt gttatccctg
    29221 aaggagtaac agttactcaa gacggaaaca acgttacagt taaaggacca aaaggtgaat
    29281 tgactcgtac tttttcagca gatatcaaaa tgaatatcga aggaaacgaa gtaactttca
    29341 ctcgtccaaa cgacagtaaa gaaatgaaaa caattcatgg aacaactcgt gcgaacttca
    29401 acaacatggt tgtaggtgtc agcgaaggtt tccaaaaagc gttagaacta atcggggttg
    29461 ggtaccgtgc acaattacaa ggatcaaaac ttgtattgaa cgttggttac tcacatccag
    29521 tagaaatcac accaccagct ggagtaactg ttgaagtacc agctaacaca caagtaattg
    29581 ttaaaggtgc gaacaaagaa gaagttggcg aattagctgc aaacattcgt ggaactcgtc
    29641 ctccagaacc ttataaaggt aaaggtattc gttatgttgg cgaatatgta cgccgtaaag
    29701 aaggtaaaac tggtaaataa tcgtttagaa aaattgcata gaagccagca gcaggaaaaa
    29761 ggcatttggc tgaagtttac cttcaaaaaa atacattttt acttgtgccg cagcttctat
    29821 ttttcgaaac gtatttactg cttgaaatag tagtacaata aaaataaaga ggtgacaatt
    29881 gtgattacaa aaccagataa gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac
    29941 aatatctctg gtactgctga gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc
    30001 tacgctcaaa ttattgatga cgtagcgggt gtaacgctag caagtgcctc taccttggat
    30061 aaagaaattt caggtggaac aaaaacagaa gcagcacaag ctgtcggtaa actagttgct
    30121 gaacgtgcag ctgaaaaagg cattaaagta gtagtctttg accgtggtgg atacctttac
    30181 catggccgtg tgcaagcttt agctgaagct gctcgcgaaa atggactaga attttagaag
    30241 aaggaggaat accattcatg gtttatatcg atccaaaaca tttggaatta gaagaccgcg
    30301 ttgttgcaat taaccgtgta acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg
    30361 ctttagttgt tgtcggtgac aaaaacggac acgtagggtt tggtacaggt aaagctcaag
    30421 aagttcctga agcaatccgt aaagcaattg aagatgcgaa gaaaaactta gttgaagtac
    30481 ctatggttgg ttcaacaatc ccacacgaag taatcggtgc ttttagtggt ggtagaatcc
    30541 taatgaaacc tgctgtagaa ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat
    30601 tagaattagc tggggtagca gatatcactt ctaaatcttt aggctcaaat acaccaatca
    30661 acgttgttcg cgcaacagtt gaaggtttga aacaattgaa acgtgcagaa gaagttgcag
    30721 aacttcgcgg caaatcagtt gaagaattaa tcggataagg aggacaacaa taatggctga
    30781 attaaaagtt actttaaaac gcagtattat cggacgtcct caaaaccaac gcgatacagt
    30841 taaagcgtta ggtcttacaa agattaacag ttcagtggtt aagccttcta atgaagcaat
    30901 taaaggcatg atcaatactg tttctcacct agtggacgtt gaagaaattt aatttagaaa
    30961 aatcataact agaacattaa ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg
    31021 ctgaaggttc acgccaagta cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa
    31081 aaactgccgg acgcggacaa aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag
    31141 gatttgaagg gggacaaaca ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg
    31201 tgaaccgtaa agactatgca gtcattaatc ttgatgtatt gaaccgcttt gaagacggag
    31261 ctgaagtaac tcctgttgct ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa
    31321 tcaaagtatt agctaatggg gaactaaaca agaaattaac tgtgaaagca gcaaaattct
    31381 ctaaagcagc tcaagaagca attgaagcgg ctggtggatc aatcgaggtg atctaatgtt
    31441 tagactatta aaagatgctt ttaaagtaaa agacattaga tcgaaaatcc tatttactgt
    31501 atttgtgttg tttgtatttc gcctaggtgc acatattact gtaccaggcg taaatgctgc
    31561 ttcattgcaa aacctttctt ctttgccatt tttcaacatg ttgaacttgg taagtggtag
    31621 tgcgatgcaa aacttttcca ttttctcaat gggggtttca ccatacatta ctgcttctat
    31681 cgtcattcag cttcttcaaa tggacatcgt gcctcgattt gttgaatggt cgaaacaagg
    31741 ggaagttggt cgtaaaaaac tgaatcaagc gactcgatat ctgacattag tattggcatt
    31801 tatccaatct gtcggtatca ctgctggatt caatcaatac gctcaattag gtttcgtgaa
    31861 taatcctaac gtagcaactt acgtaacgat cggtttgatt ctaacagccg gtacgatgtt
    31921 tgttacttgg ctaggagaac agatcacaga aaaaggtatc ggaaatggcg tatcaatgat
    31981 catttttgca ggtatcattt ctagattgcc tgaaagtatc agagaaattt atgaagatta
    32041 ctttataaat attgatcctt cagacatatg gaaatctgct atttttgtgg ccattttact
    32101 agtagcggtc ttgttgatcg tgacattcgt aacatttttc caacaagctg aacgcaagat
    32161 cccgatccaa tatacgaaac gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa
    32221 agtaaatgca gctggagtta ttccagttat ctttgctagt tcgtttattg ctacgccaaa
    32281 tgctgtttta caagcattga gcggtagatt cggttctgaa aactggtaca aagtgatgat
    32341 ggagatcttt aactataata cagcacctgg tgctacgatt tatacgattc tgatcgttgc
    32401 cttcactttc ttctacgcct ttgttcaagt aaaccctgag aaattagcgg aaaacttgca
    32461 aaagcaagga agttatatac caagcgtacg ccctggtaag gggactgaag agtatgtctc
    32521 aagattactg atgcgtttaa gtacagttgg tgcagtcttc cttggtttag tggcattgct
    32581 tccaatcatt gctcaaatgg tttgggatct tccacaatca atcggattag gcggtacaag
    32641 cttactgatc gttatcggtg tagctttaga aactgcgaaa cagttagaag gcttaatgct
    32701 gaaacgtaaa tatgttggat tcattgagta agaaagcgtg ttgaggtttc ccctcaacac
    32761 acgttctcac acatttatta ggagggaaaa caatgaacct cattttaatg gggctgccag
    32821 gcgcaggtaa aggaacacaa gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct
    32881 caacaggaga tatgttccgc gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa
    32941 agtcttatat ggataaaggt gcattagttc ctgatgaagt aacaaatgga atcgtgaaag
    33001 agcgactagc tgaaccagat acagagaaag gcttcttatt agatggcttt ccacgtacgc
    33061 tagatcaagc aaaagctctg gacgcaatgc taaaagattt gaataaaaaa attgatgctg
    33121 tcatcgatat ccatgttggt gaagaaatct tggtcgaacg tctagccggc cgatttatct
    33181 gcagcaattg tggagcaact taccataaaa ttttcaaccc tacaaaagtt gaagacacat
    33241 gtgatcgttg cggtgggcat gaattctatc aaagagaaga tgataaacct gagacggtta
    33301 aaaatcgtct ggctatcaac atcaaaaata gtgaaccaat cttggcttat tataaagaac
    33361 aaggtttgct aaacactatt gatggtgctc gtgaaatcga tgctgtattt gcagatgtac
    33421 aaaaaatcat tgaaaaatag cacgattgcc cgtagccgac ttgcattatg cagttactct
    33481 tgtcaagttc aataaattat gatagaatat gtcagtccga cttctagaga atcctctaga
    33541 tgatccattt tgagaatttt gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa
    33601 atgcgaaaca agtacaagga ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa
    33661 ggtacagtcg tcgaaacttt gccgaatgca atgtttaaag ttgaactaga aaacggacac
    33721 caagttcttg ctactgtttc aggtaaaatt cgtatgcatt acatccgtat cttacctggt
    33781 gacaaagtaa cggttgaatt gtctccatac gatcttaacc gtggccgtat tacttatcgc
    33841 tttaaataat tgtactccgt aaaactacag gaggtattat catgaaagta agaccatcag
    33901 taaaaccaat gtgcgaacat tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt
    33961 gcccagcaaa tccaaaacat aaacaacgtc aaggataatt ggaggtgcta taaatatggc
    34021 tcgtattgca ggagtagata ttcctcgtga taaacgtgta gtagtttcac ttacttatat
    34081 ctatggtatt ggtaacacta ctgctaaaaa gatcttagct gatgctggtg tatctgaaga
    34141 tgttcgcgtt cgtgaattaa cgaatgaaca aacagatgct atccgtgcag aagtagataa
    34201 attaaaagtt gaaggggatc ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga
    34261 aatcggttct taccgtggaa tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac
    34321 gaaaaacaat gcacgtactc gtaaaggccc agctcgcgct atcgctggca agaaaaaata
    34381 attcttaagt gaaggaggtt aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg
    34441 tgtgaagaag aatatagaag caggtattgc acatatccat tctacattca ataacactat
    34501 catcatgatc actgatactc acggaaatgc attagcgtgg tcatcagctg gttcattagg
    34561 tttcaaagga agcaaaaaat caactccttt tgccgctcaa atggcagcag aaactgctac
    34621 aaaagcagca atggaacacg gattaaagac tgttgaagta acagttaaag gacctggttc
    34681 tggacgtgaa gcagcaattc gttcattaca agctacaggt ttagaagtga ctgcaattcg
    34741 tgacgtgact ccagttcctc ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg
    34801 agtgccgctc attttgagtt ttatcagtaa cgtcattgaa caagacactt ttcgttttga
    34861 aaggggtaaa gataagaatg attgagttcg aaaaaccaag aatcgcaaaa attgatgaag
    34921 aaaaagatta tggcaagttc atcgttgaac ctcttgaaag agggtatggg actactttag
    34981 ggaattccct acgtcgtatt ttattatctt ctttgccagg tgctgccatc actaatattc
    35041 aaatcgatgg tgtactgcat gaattctcta ccatcaaagg tgttagagaa gacgtcactc
    35101 aaatcatctt gaacattaaa ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc
    35161 ttgaaatcga tattaccgga cctgctacag taactgctgg cgatattatc gttgatagtg
    35221 atgtagaaat tcttaacaaa gatatgtaca tctgtagtgt ctctgaagga gctacgttcc
    35281 atgctcgctt aacagtgaaa cctggacgtg gttatgttca agcagatgaa aataaaaaag
    35341 aagatatgcc aattggcgtt cttccagtcg attcaatcta cacacctgtt cgtcgtgtga
    35401 actatcaagt tgagaacaca cgtgttggac accgtgaaga ttttgataaa ttgacgatgg
    35461 agatttggac agatggttca attgaaccgt tggaagcaat gagtttgtct gcgaaaatca
    35521 tgactgaaca tttggatatt tttgtgaact taactgatga agcaaaaaat gctgaaatca
    35581 tggtcgaaaa agaagaaaca caaaaagaaa aaatgttgga aatgacgatc gaagagttag
    35641 acttgtctgt tcgctcttac aattgtttga aacgtgcagg aatcaatact gtacaagaac
    35701 ttacaaataa atctgagcct gaaatgatca aagtccgtaa cttaggacgt aaatcattag
    35761 aagaagttaa agcaaaattg cacgacttag gtttaggttt acgtaaagaa gattaaaacc
    35821 ttttacgaag gagggaaacc aacgtgagtt atcgtaaact aggacgcaca tctagccaac
    35881 gtaaagcgat gttgcgtgat ttaacaactg atttattaat caacgaacgt attgtgacta
    35941 ctgaagctcg tgcgaaagaa gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac
    36001 gcggagattt gcatgcacgt cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg
    36061 tgcgtgaaga aaatgaggac atcgtcattg aatcagcttt acaaaaactg ttcaacgata
    36121 ttgcacctcg ttacgcagaa cgtcaaggcg gatacacacg cattttgaag acagaaccaa
    36181 gacgcggtga tggtgcacca atggttgtct tagaacttgt ctaaaatcac actaataaca
    36241 tcactttata gatgattctt ttagagtgtt atgatgttgg aagatatttt tccgagtcta
    36301 gctcagcgct gctcctcaag cgaatatcgc ttggggagta agcactcatc ataaagtgtt
    36361 tttttatacc ttcaattacc tgtcatgaca gatatgtttg aaggcataaa aaaacatttt
    36421 aagcgagaaa taattgaaaa ttttgtcttt ttttgatagt gttagtagga actttaggat
    36481 gaagaaggag taatcatgga gccaattatt gaattagaaa agataaatta taaataccag
    36541 ccagatgatc ttcgccccgc attgaaagat gtctccttta caattgataa aggtgagtgg
    36601 atcgctatta ttggacacaa tggttcagga aaatcgacac ttgccaaaac gatcaatgga
    36661 ttgcttctgc cagaatcagg gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat
    36721 atttggacga tccgacagat ggttggaatg gtttttcaaa atccagacaa tcagtttgtt
    36781 ggttcgacag tagaagatga cgttgctttt ggtcttgaaa atcaagggat tcccagagaa
    36841 gaaatgctag tgcgtgtgaa agatgcactt gagaaagtta gaatggctga gtttgcttca
    36901 cgcgagcctg ctcgtttatc aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt
    36961 gccttacgac ctgatatcat tattttagat gaagcaacca gtatgctaga tccagaaggt
    37021 cgtgaagaag tgatctctac gatcaagaag atcaaagaag aaagtcaatt gaccgttata
    37081 tctatcactc atgatattga tgaagcagct aatgccaatc ggatactagt gatgagacaa
    37141 ggcgaacttg tccgtgaagg tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc
    37201 gacttaggtc tagatctacc atttcctgaa aaactgaaga gcgcactgaa agaacgtggg
    37261 gttgatgtgc ccagtgaata tatgacagaa gaaaggatgg tggattggtt atggacatcc
    37321 gttttgaaca agtagacttc acttatcagc cgaatacacc atttgaacaa agagccttat
    37381 ttgacatcaa tatgacgatc aaagaaaaca gttatactgc attagtaggg cataccggaa
    37441 gcgggaaatc aaccttactt cagcatttga atgcgcttgt taagccaaca agcggtacag
    37501 tccatattgg agaacgagat attcagccgg atacggataa taagaattta aagcccatcc
    37561 gaaaaaaagt tggcatcgtc tttcagtttc cagaagcaca gctattcgaa gaaacggtag
    37621 caaaagacat tgctttcggt cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc
    37681 tagcaaaaga aacattagaa caagttgggc tggatgaaag ctatttggaa cgttcgccat
    37741 ttgaactttc aggagggcaa atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc
    37801 cggaagtact tgtattggat gaaccaacgg caggacttga tccccagggt agaaaagaaa
    37861 tgatggagat gttttggcgt cttcataagg aacagcagat cactattgta ttagttacgc
    37921 atttaatgga tgatgtagcc aattttgcag attatgtcta tgtgttggaa aagggacgaa
    37981 tcgttaatag cggggagccg caagaggtct ttcaaaacat tgaatggcta aaagaaaaac
    38041 aactaggcgt acctactgcc accgagtttg cagaagaatt aatggctaaa gggatgaact
    38101 ttgctacctt gcctttgaca gctgaagaat tagcagacgc tatcgtccat tatgctggag
    38161 gaaatgccca tgatgaataa attgatattt ggtcgttata tgcctggaga ttccttgatc
    38221 catcgattgg atccccgagc taaattatta gccagttttt attttatcgg aatcattttt
    38281 ttagcaaata attgggaaac gtaccttttg ttggcatttt tcacattact ttctgtcttc
    38341 ttgtcaaaag tagatttgaa attttttatc cgaggcattc gcccattgat ctggctaatc
    38401 ctcttcactg tagctttgca ggtcttgttt acaagtggcg gagaaactta ttggagttgg
    38461 ggtgttttcc atatcactga atttggtgtg atgaacggcc tctttatttt ctgtcgattc
    38521 gtgttgatca tttttatgtc gactctactc acgcttacta caccgccttt ggaattatcg
    38581 gatgcgattg agtacttgct tcgtccattg aaagccgtac ggttcccagt ccacgaaatt
    38641 tcattgatgc tgtcgattgc tcttcgtttt gtgcctacat tgatggacga aacagaaaag
    38701 atcatgaacg ctcagcgcgc tcgcggtgtt gattttggtg aaggaaacct agttcagaaa
    38761 atgaaagcag tcattccttt gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa
    38821 gatcttgcga ctgctatgga agcaagaggc tacaaaggtg gggaaggaag aacgaaatat
    38881 cgtgtattgc attggcataa tagagacacg atggttatca ttgcttttgt attattaact
    38941 gtgctactga tctttttgag aggataaacg ttcagtgggc cgctatctat tcttttcttg
    39001 ctgatctgtt tgcaagaaag gatgcgagca gtgtatgaca agaaatatta gggtctgtgg
    39061 cagactaagg tcacaggcct ctttctttta tctgaaaaga aagaacgaaa aaattttata
    39121 aaaaaatcat tattccagat aaccttttag gaacaaagga gagaaaaaca tggtacgtta
    39181 caaagcaatc attgcatacg acggaacaaa ttttaatgga ttccaaaaac agccaaatgg
    39241 gcgtactgtt caagaagaag tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat
    39301 cacggtcttt ggttcgggaa ggacagatgc cggagtacat gcgattgggc aagtaatcca
    39361 ttttgattat ccagaggagc gtccgttaga acgaatgcgt ttcgcgctag atacccagtc
    39421 accagaggat attgctgtaa gacaagtaga aattgtttca gaagacttcc acgcaagata
    39481 tttggtcaaa gaaaagacgt atcaatttcg agtggatata gggaaaccac gcagtccttt
    39541 tcgacgccat tatgcaagtt attttcctta ccctcttgat ttggaaaaaa ttcagcgggc
    39601 gcttcctgat ttattaggga ctcacgattt cacctctttt tgtgcatcag gaagctcgat
    39661 agaggataaa gtacgtacga tctacgaggc taagatggag gtaaatgaaa caaaagacga
    39721 attgttgttt acatttaggg gaaatggctt cttgtataag atgatacgga tcttagtagg
    39781 aacgcttttg aaaattggca atggccgatt gcctgaagat agtatcccag atattattgc
    39841 gaaaaaagat cgtaatgcag caggacctac agcacatcct gaaggacttt atttgtatga
    39901 agtgaaatat gaataagagg ttgtgacaga agtggtcagc ttcaagaaat aagaaggaat
    39961 ttacgaaaat tacttctcaa aattttgcaa aatttcggct tattttcgaa ggagctactt
    40021 ctggaacacc gtgaatcaga ggttgtgaaa aagctgccct gcatcaagta ataagaacaa
    40081 ccaaacaaaa atagctcgtc atatttttcg tttggttggg cttattaccg cagatgcaag
    40141 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg ttttgacctg agtattagag
    40201 aaacaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata ccgaaggtca
    40261 gctgattgaa caccgtttaa gaaagaggtc gtgagcctgt catttaactc tgagtcacaa
    40321 gtaacaattt caaaaaacag cttctcatct ttgatgaata tcgtgacttg tggacgaggc
    40381 gttgataagt gaatcatctt tttgttacct attttttctg taaccaaaaa acgatggaac
    40441 gttgttttga ttgcccttaa tgaggacagt cggaacaggt ttgtcatttt tatatttaaa
    40501 tggcacaaat ttttctaatt atttgtcatt tagcgctgaa aatttttgct atttatctaa
    40561 aaaagtgata actacttaag atgaagcaga atatttacgc catacggcat acaaccgcga
    40621 aaaatataaa aaaaagaaaa cgattgaacg cgtttttgca gtcgcaaagg aaaagcatgg
    40681 aatgcgctac atcaagtatc gagacttgga aaacaatcag gtgtatacgc tgattgtttt
    40741 tgctgcaatg aatctgaaaa agttgtccac ttggcagttg gaacatatgt cttcatatct
    40801 tttattatac aaaaatagca aaagcaccag tctacactct tagtaaagag gaaactgatc
    40861 cttttgtcta cattcttaaa gtacttaaac taagtacttt attttactct ttaggattga
    40921 tacagtagca tctatccctt tattaataat cttttatttt tttaaataaa gatgttgtct
    40981 ccatcttcag gcactaaaac tttttcggaa attccttttt ctttattgaa agactttaat
    41041 tcttctcttg acagtcccca atggtttaca gcttccatat gaacggcaat aattgtggaa
    41101 tctggtattg ctttatgcaa ttcatataaa tcgtctttcc ccataataag agattctcca
    41161 actaaaaaat gattatctcc agcatttgct actatgattt ctggattata ctgatcaata
    41221 gtttgtttaa cagcttcata ccaaactgta tctcctgtaa agtatagtgt cttctcagtc
    41281 gaatgtttga aaacaatacc gcaaacatta cctacataat tcaagatttc tcctctgcca
    41341 tgtttgcctt ttgttttaac caattgtata gattcaaaaa ctgtgtcttc tcgtagtacc
    41401 tccacatttt taaaaccata tagtcgaatt tcattcgcat cattttcatc ttgcacaaat
    41461 aattttattt ctttaggtag gacctctttc gctatatcgt cccaatgatc tagatgtaaa
    41521 tgtgttacaa taacagcatc aataccgttg ataatatctt cgactggcat tggcaaatca
    41581 actagaggat tcatttgatc ttttcttggt gatggaaaag gacctttcga aataaatggt
    41641 ggaagtgttc ctttttccgc taacattgga tcaattaaaa attttttacc tccatagtct
    41701 aagatagctg ttgcattacg aatgtgatgt atattcataa acgttgtctc ctttttgttg
    41761 atatgaaata tttttagtat aattcaattc catacaataa ataaaccgaa caaaaaaagt
    41821 cttttactat ttttacttaa tttatgaaag gttgtttacc atgttagacc aaacagatat
    41881 aaatatcatt gaggaactat caaaaaatag tcgaatcaca atgaaagaac ttggagaaaa
    41941 agtacatttg actggacccg ctgtctcggc gagagtgaca aaactagagg aaagtggagt
    42001 tattgaaaat tatacagtaa aattaaatat ggaaaagcta gggttttcga ttcacgcttt
    42061 tttaattata attacgcaaa atttaaatca taaaccctat ttatcattta ttaaaaatca
    42121 tgaaaaattc ttgattagaa attataagat tagtggaggg ggctgttatc ttttggaatg
    42181 tagatttact tcaaaaaaat caatgaacag ctttttggaa cagcttaatg aatatgcgaa
    42241 ttataaatta tctattgtta tagattaata ataaaactag cagatggtaa aaatactaat
    42301 atgcgagaca cacattcaac cataactata taacttatca atttaaaact aaactgatgg
    42361 aaacaagtaa ggcaaaaaca tttatatttt gccctactta tttttttaat taaattcatt
    42421 gatgattcat atttagaaaa ttctatgaag gccaaatgag tatcctgtca cttttttaga
    42481 taaatagcaa aaattttcag cgttaaatga caaataatta gaaatatttg tgccatttaa
    42541 atataaaaat gacaccaagt atatcatgaa cgcacaaaaa gaccaacagg gtataccctg
    42601 ttggtctaaa taa
//
LOCUS       DAYLZO010000009        42508 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.009, whole genome shotgun sequence.
ACCESSION   DAYLZO010000009 DAYLZO010000000
VERSION     DAYLZO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.009"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(95..1237)
                     /locus_tag="ACGVJG_000452"
     CDS             complement(95..1237)
                     /locus_tag="ACGVJG_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6556438.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGICLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(1242..1427)
                     /locus_tag="ACGVJG_000453"
     CDS             complement(1242..1427)
                     /locus_tag="ACGVJG_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFJ6556439.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1781..2026)
                     /locus_tag="ACGVJG_000454"
     CDS             complement(1781..2026)
                     /locus_tag="ACGVJG_000454"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556440.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(2020..2421)
                     /locus_tag="ACGVJG_000455"
     CDS             complement(2020..2421)
                     /locus_tag="ACGVJG_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFJ6556441.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(2606..4414)
                     /gene="uidA"
                     /locus_tag="ACGVJG_000456"
     CDS             complement(2606..4414)
                     /gene="uidA"
                     /locus_tag="ACGVJG_000456"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFJ6556442.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KLITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWKERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLQKRWSSIPNFSYKK"
     gene            complement(4437..6203)
                     /locus_tag="ACGVJG_000457"
     CDS             complement(4437..6203)
                     /locus_tag="ACGVJG_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFJ6556443.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IISDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIEPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(6216..7010)
                     /locus_tag="ACGVJG_000458"
     CDS             complement(6216..7010)
                     /locus_tag="ACGVJG_000458"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFJ6556444.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYYQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGEYVDIEQKFIDTFWKNLTNK"
     gene            complement(7020..8396)
                     /locus_tag="ACGVJG_000459"
     CDS             complement(7020..8396)
                     /locus_tag="ACGVJG_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFJ6556445.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLIIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQFKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANIQTGDVLEKLYNASTLIPSICFFIVGLILFFLYPLSKDKVLENC
                     ELLKQKYK"
     gene            complement(8516..9163)
                     /locus_tag="ACGVJG_000460"
     CDS             complement(8516..9163)
                     /locus_tag="ACGVJG_000460"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFJ6556446.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(9256..10683)
                     /gene="uxaC"
                     /locus_tag="ACGVJG_000461"
     CDS             complement(9256..10683)
                     /gene="uxaC"
                     /locus_tag="ACGVJG_000461"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFJ6556447.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLGYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(10757..11836)
                     /gene="uxuA"
                     /locus_tag="ACGVJG_000462"
     CDS             complement(10757..11836)
                     /gene="uxuA"
                     /locus_tag="ACGVJG_000462"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFJ6556448.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLQTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(11839..13470)
                     /locus_tag="ACGVJG_000463"
     CDS             complement(11839..13470)
                     /locus_tag="ACGVJG_000463"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFJ6556449.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSNIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            13776..14447
                     /locus_tag="ACGVJG_000464"
     CDS             13776..14447
                     /locus_tag="ACGVJG_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6556450.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQHGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKINNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKSI"
     gene            complement(14607..14798)
                     /locus_tag="ACGVJG_000465"
                     /pseudo
     CDS             complement(14607..14798)
                     /locus_tag="ACGVJG_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(15164..15505)
                     /locus_tag="ACGVJG_000466"
     CDS             complement(15164..15505)
                     /locus_tag="ACGVJG_000466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556451.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            complement(15522..15689)
                     /locus_tag="ACGVJG_000467"
     CDS             complement(15522..15689)
                     /locus_tag="ACGVJG_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556452.1"
                     /translation="MQTLINALQGKNYDYAVKILLDVAKRSLTPAQFAKFNAATIAAG
                     IAINAISCWGK"
     gene            complement(15689..16096)
                     /locus_tag="ACGVJG_000468"
     CDS             complement(15689..16096)
                     /locus_tag="ACGVJG_000468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556453.1"
                     /translation="MSRFFKFFSLLGVMLIGLSTLVGAGTSVYAAEKVNEVPDIETQR
                     QQAVESLKFYLEEAGYVDTETHQYIVTDLEAIQKKAELDGSEGIAGKFILENFVQPTG
                     KRQKRDLQKYAACVVMNSLPFGVLFGIYLIVKI"
     gene            complement(16597..16791)
                     /locus_tag="ACGVJG_000469"
     CDS             complement(16597..16791)
                     /locus_tag="ACGVJG_000469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556454.1"
                     /translation="MKDQKTNNKTGERSACSWAFTGAGLLHSGIYTAAALGLSLTGVG
                     AAVIPFAVGTAYGIAGAAIC"
     gene            complement(16805..17143)
                     /locus_tag="ACGVJG_000470"
     CDS             complement(16805..17143)
                     /locus_tag="ACGVJG_000470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556455.1"
                     /translation="MKRIVKIFGILGVFLIGLSTFVSAGISVDASETNNFLVKQQETE
                     INIIQNNLATHKDSKGNRLVYIIDESDLKSSLDRINSEITVSEIQQYLNEFNRSIQKK
                     WPWMANRSAS"
     gene            complement(17187..17519)
                     /locus_tag="ACGVJG_000471"
     CDS             complement(17187..17519)
                     /locus_tag="ACGVJG_000471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556456.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRKEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLVWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(17533..17790)
                     /locus_tag="ACGVJG_000472"
     CDS             complement(17533..17790)
                     /locus_tag="ACGVJG_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556457.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KVYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(17985..18521)
                     /locus_tag="ACGVJG_000473"
     CDS             complement(17985..18521)
                     /locus_tag="ACGVJG_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFJ6556458.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLTTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDYMT
                     DRCIKDNSIDVDPHVQMKTTGKLHQVTQDIENPDQYLLFGEETTNEVTQFVLLQLKVD
                     QEAQKIDAIKIYYVRSVY"
     gene            complement(18542..19633)
                     /locus_tag="ACGVJG_000474"
     CDS             complement(18542..19633)
                     /locus_tag="ACGVJG_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFJ6556459.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNWE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESTYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKDCYDYYGFLVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSDGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(19641..21851)
                     /locus_tag="ACGVJG_000475"
     CDS             complement(19641..21851)
                     /locus_tag="ACGVJG_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFJ6556460.1"
                     /translation="MAYIKRKEKKWCVWLFLTVGFGLFFVGGLVYAVEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPAFDYMIMNTLKSFLSLMFQKSFYSVIILVAFLIY
                     NIVDDLILMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGGQVINPFELGGRSNQDLPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIHQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQRQIQRSSESKPSISVP
                     KSLQPAFSSFNNPVEQSKKIEKKNETLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(21867..24395)
                     /locus_tag="ACGVJG_000476"
     CDS             complement(21867..24395)
                     /locus_tag="ACGVJG_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6556461.1"
                     /translation="MVLVKEPVKALKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFYDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRMPKEHKGLLQLEDELGTSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEYKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTTIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAIGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQIEEQDTLIMQ"
     gene            complement(24449..24859)
                     /locus_tag="ACGVJG_000477"
     CDS             complement(24449..24859)
                     /locus_tag="ACGVJG_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFJ6556462.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFFIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFANKYVYLED"
     gene            complement(24872..25090)
                     /locus_tag="ACGVJG_000478"
     CDS             complement(24872..25090)
                     /locus_tag="ACGVJG_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFJ6556463.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(25087..26079)
                     /locus_tag="ACGVJG_000479"
     CDS             complement(25087..26079)
                     /locus_tag="ACGVJG_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFJ6556464.1"
                     /translation="MKKRQLRRKEKVYTEKVPNQRKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSQSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQEILKKEFYAQNFIQEPGSVSGIRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMDPENKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKTFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDRKGDERK"
     gene            complement(26158..27069)
                     /locus_tag="ACGVJG_000480"
     CDS             complement(26158..27069)
                     /locus_tag="ACGVJG_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFJ6556465.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLSDVETELNGGTTEKPAEPEHLQPEKPEKEPVAPEPEIPESTLPTDPIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSETNTDSSSTEQPTMSFTSEEPKEEPKQPAEKSEAIPK
                     ENKPIVPAKEVTVSVTPNGEITTNNSQGMSVPIVTSNVEELTHIPTPTTPLKAEGGQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVLS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(27088..27432)
                     /locus_tag="ACGVJG_000481"
     CDS             complement(27088..27432)
                     /locus_tag="ACGVJG_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556466.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLGPRTERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(27452..28009)
                     /locus_tag="ACGVJG_000482"
     CDS             complement(27452..28009)
                     /locus_tag="ACGVJG_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556467.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIRVIKKLCTIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFHKREKNQMSAEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEKLMMDFSDLYRGYECSVIQER"
     gene            complement(28089..28589)
                     /locus_tag="ACGVJG_000483"
     CDS             complement(28089..28589)
                     /locus_tag="ACGVJG_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556468.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPKEYPIEKRELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPINFLKKC
                     HLRILP"
     gene            complement(28590..29135)
                     /locus_tag="ACGVJG_000484"
     CDS             complement(28590..29135)
                     /locus_tag="ACGVJG_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556469.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGTLKFIRFYRFSLPVPAILAVK
                     KGCDKMKIYENCVAALSNEKPLVEEISLATVTWEMNRQPTEEQTYLSLEEIELELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(29132..30388)
                     /locus_tag="ACGVJG_000485"
     CDS             complement(29132..30388)
                     /locus_tag="ACGVJG_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFJ6556470.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKKKIPEDFEKRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKIVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKQTVTQNVLVQFSGQGCREYEEVMREQRTD
                     WRTWLYSIWKNQGKLEAGYKRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKKFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISEIEAL
                     TKYGIYNRYELRLANEKATQAVEAFILDHSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIENAELNDEQQSYLEWMGTSGGKIA"
     gene            complement(30751..31065)
                     /locus_tag="ACGVJG_000486"
     CDS             complement(30751..31065)
                     /locus_tag="ACGVJG_000486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556471.1"
                     /translation="MGEVLYRVSSAAGEISPDFAVRRLYEWINKVEYYTKGSYVFRRI
                     ERETLFVTRNQIVLTKEDILRFRQVYRLCKEKNIQLRLAILQCFAPEQYKELQEKEDS
                     LV"
     gene            complement(31087..32550)
                     /locus_tag="ACGVJG_000487"
     CDS             complement(31087..32550)
                     /locus_tag="ACGVJG_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFJ6556472.1"
                     /translation="MEPHFFKYRGRRIRYFNRYLWFCYYGFFLLPFLILLSWFSWKVF
                     YPRLNTYSNWQMYLIPGISIVMYLLLVSGLILGLMRWSRLKEGYFASVYWRQLLARML
                     IDNRFVYTKKQVGEKQTKEKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGSLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNLLI
                     SGAIGGGKTYLLYSLIKACLDVGTVDICDGKAADLAALGDIDVFKGHVFYKTNEEMII
                     CLRNALQEMNQRYTYMKTMKEPKYEPGKNYAYYGIPPHFIFFDEWAAFYGSLDMETRN
                     KVDKLIQPLVLKGRQAGMYVILAMQRPDAEYFPSGVRDSLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIEEFKKFDTMIVQDFSV
                     LKEEAFTSYEEQIDRQAQSLKGNTKTNGVDEESYTLL"
     gene            complement(32575..32931)
                     /locus_tag="ACGVJG_000488"
     CDS             complement(32575..32931)
                     /locus_tag="ACGVJG_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556473.1"
                     /translation="MNLDKTNQTLREIMVCCQNIQQLIEYFGDNPTIFIQTNEYICAV
                     IGQCMFLRDLVRFSLPYQWKQDHQEVVWIRVVRICNMCAHPYKNRLNLFRLWFFIKCD
                     IPVLVAFCQKFLAQEK"
     gene            complement(32997..33449)
                     /locus_tag="ACGVJG_000489"
     CDS             complement(32997..33449)
                     /locus_tag="ACGVJG_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556474.1"
                     /translation="MGLKFPNNYDITEALDETCIGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNSNPKQSQEKGLDQKQPSADSKEKEKK"
     gene            complement(33451..33816)
                     /locus_tag="ACGVJG_000490"
     CDS             complement(33451..33816)
                     /locus_tag="ACGVJG_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556475.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            complement(33833..34087)
                     /locus_tag="ACGVJG_000491"
     CDS             complement(33833..34087)
                     /locus_tag="ACGVJG_000491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556476.1"
                     /translation="MEKELLLGFYRKENNGGKKNMEKKNINICFLSGKECTYEECTDI
                     SVHERDGMLIVDMSPSDEPFVVKHYFPLCNIESFRIDCEF"
     gene            complement(35005..39300)
                     /locus_tag="ACGVJG_000492"
     CDS             complement(35005..39300)
                     /locus_tag="ACGVJG_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFJ6556477.1"
                     /translation="MYAKTKMNWLKALALVALSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKLNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KNMNGKVQAEIKDLDPGKYVLKEIKAPKGYALSDETIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKIANKDLVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKERYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVT
                     FTLYNGKGQAIGKYTTDQNGEILVKGLVANNYYFTENVPLESFQPNTGKQAFTITFRQ
                     NAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQEGKEITSILTFIADKANGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAVHVDINDDNQSVRVNNPTVHTTAVFEDGLTIADPTGLFTLNDTVEINE
                     ILPGKAYLVKGILMDKATGEALLVHGQPVESAVGFIAKAENETVIVPFTFDLTGLNGR
                     EMVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLIVGKTYTVNGVLMDKVTEKPVLVDGKEVSSTLTFVAETTDGTVNLDFILD
                     AHALRSKEVVVFEDLFREGVLLASHADITDKDQTIKIVSPTITTKATNANGGKEILAL
                     PNQKVVEWVQVNGLAIGQIYKLIVQGYLTPDGTPFEGTHAEKVFMADKEMMEFLFEFI
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQFVEAPKPPQPVK
                     PNEPQPVKEVKQAAMVPMLPKTGSEDNVLWIMIGMIFVLAAGLIGYDLYKVRD"
     gene            complement(39447..39686)
                     /locus_tag="ACGVJG_000493"
     CDS             complement(39447..39686)
                     /locus_tag="ACGVJG_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556478.1"
                     /translation="MIDISIPEDLKPDPATIGNSGMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMIFGDGEVANKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(39776..40018)
                     /locus_tag="ACGVJG_000494"
                     /pseudo
     CDS             complement(39776..40018)
                     /locus_tag="ACGVJG_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40131..40367)
                     /locus_tag="ACGVJG_000495"
     CDS             complement(40131..40367)
                     /locus_tag="ACGVJG_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556479.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFVESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(41380..42291)
                     /locus_tag="ACGVJG_000496"
     CDS             complement(41380..42291)
                     /locus_tag="ACGVJG_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556480.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEISKESNRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFNKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT        13093 a         8767 c         5856 g        14792 t
ORIGIN      
        1 ataccgacag ctagcttata acccaaggta tatcaacata aaaaaaaaaa aaaataggct
       61 gtgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc gtaatttcat
      121 ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa gtagttttaa
      181 tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt tcaattaaaa
      241 aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct gcaatttcag
      301 ctaatttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca tcaaaagaaa
      361 aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt tttaaatctt
      421 caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt gtttcagcaa
      481 attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat ctgccattta
      541 ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca cctgtaacaa
      601 aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga taaacactat
      661 tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc tgagttttta
      721 ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt gcccttttaa
      781 aaactactct caacttattg aatatctgtt taatataatt tggtgataat ggttgctcaa
      841 tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt atatgaattg
      901 gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt aaaatatgat
      961 ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa aaatactctc
     1021 gataaaaatc atgaaacgtt attccttgca gacaaattcc ttgtgaatca gaaaaacttt
     1081 gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct cttctggtca
     1141 cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac caagtgcctc
     1201 tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc ccaagaaata
     1261 tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt acgataaaaa
     1321 aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt agcatagtac
     1381 tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat agcaccttcc
     1441 ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag gaatttcacc
     1501 tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt aaatcatcgc
     1561 tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta cagcaagaaa
     1621 tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa tctatttaat
     1681 tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa ctattagaag
     1741 attcttttaa acagaaaaag tggaaaggag atttttttaa ttagttaatt ttaaatttta
     1801 agatcgcttc tattaattta atttctagct gacttatcat atattcatct acataggtaa
     1861 cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata taactatgat
     1921 aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat aagataatgg
     1981 atagtggaac taataggtac tgccgctttt tggatttatc taccataggt cataccccat
     2041 tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc tcatattgag
     2101 aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga taatctttct
     2161 tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa ttaatatttc
     2221 cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat acctgccttc
     2281 atttgaatct gccaatggtt cctcattatc ctttatgtta gcatttcgtt ttcgaataga
     2341 cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt gtatagatat
     2401 gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca ctaatacaac
     2461 aatcagagag cccctttttt tcgaaaaaac tagaaaaaaa cataaaaata tttatcattc
     2521 tttaaacaga aaatctttct ataaaaaaca aaaaaacgat taggctaggc taatcgtttt
     2581 ttgaacattc ttaaaaaaat ccaaattatt ttttataact gaaattgggt attgatgacc
     2641 atcgtttctg tagatagtga gcaatcattt taggttttct atctctagta aagattcctt
     2701 tcttatttcc ttgcactctt aaaatccctt gactcgttgc aaaatctgca aaattccaca
     2761 cttgttcacc aacaaaattt tcacatgtat ctattacttc gtgatttgct tcgtaataag
     2821 ccacttgata ctcttctgta aacattgttg gtactgtatc atgaaatcca gctactgtat
     2881 ctgctccata ttctgtaaac attattggcg tatcaggaca tctttctttc catccatcta
     2941 gctctttttt caacagatat tttgcatgtg ccagatcccc tcccgaaaca taccaaccat
     3001 aataacgatt taaacataaa acatcgacaa tatctcctac cttacaaata tctggcgttg
     3061 attttagata agtaactatt gttacaggtc ttttctgcgg atcaagttct ttagttaatt
     3121 caactaaagg ttcaaaatat tctttagcac cttcggaatc cgaatctggt tcatttgcaa
     3181 tagaccacat tacaacactt gggtggttct tgtcgcgttc tatcaattcc ttgatcaccg
     3241 ttttatgaga attttctgtt cgtatctctt tccacgtatt atgtttttcc acattatcat
     3301 ctaataaagt ggccatgaaa tttaaatgta accctactgc aggtacttca tcaataacta
     3361 caataccttc tcgatctgct aatcgcataa tttcttctga gtaaggataa tgtgatgttc
     3421 taaaagaatt agcacctatc catttcatta aattaaaatc tttaatattt acagcctcat
     3481 tcaaaccacg tccattaaca tacgaatcct catgcttacc aaatccttta aaataaaaag
     3541 gtttattatt aatcaaaaat ttgccattgg taacctgaac actgcgaata ccaaattctt
     3601 ctgtataggt atcgatcact ttattatcat caattaaact aattttcata ctatataaat
     3661 aagcatctag aggttcccaa aaaattggat tcttaagctg aatcattcct ttctttcctt
     3721 cagcttcagc tacaagttga gcctcttcgt catatatcgc tattaagatt tttccggtcc
     3781 attctgtatc gatattgaat tctacacttg catccaaatc agttattctt gtattaatag
     3841 taatatcact tatatagtct ttcggagttg tatataactt aacaggtcta tgaatacctg
     3901 cataattgaa gaaatcaaaa ttaactaagt tttccttttt tatatcccca ttttcctgta
     3961 ctgtttcctt cataatcccc acaggtaaag tactatcgtc aataatattg tttacagcaa
     4021 ctgtaattcg attattcccg tcttttacaa accgattaat ttcagactca aatggtgtaa
     4081 acccaccttt gtgttgagta atcaactttc cattcaaaaa aacttttgct tcatgtgttg
     4141 cagatccaaa acgaagaact aatctttctt ctaataatct tttaggaaga gtcacttctt
     4201 tttcatacca cacccatcca acatgatttc taatgttctc tgtttcaaat aaatcattat
     4261 aagaagaggg aactgaaatc ggataactct ctttttcatt tattggttct aaagcaagtt
     4321 cttcctgtaa gtcatcattt gtatctaatt taaatctcca gagaccgctt aagtcaatta
     4381 cttgcctcgt ctctgtcata atgggataca acatataaac cactcctatc taaatattaa
     4441 ataaatgtat cctttaaacc acaataagca tctattggac ttactccctt aaattcagaa
     4501 agacccatta gtttgtccac caatatttct aaagtttctt gttttccatc ataagtatta
     4561 atataagtct ttacttgagg tacatcagcc aaatgaaacg gacactgtac agatacaaaa
     4621 attgttggaa tttcatgaat gtaaaacggt atatccggag ttcctttact tgctggccag
     4681 acaattcgtt gaactgtgcc tggattaaca ttagctatat taatgatcaa gtcatatttt
     4741 ttagttaaat ttttaattgg tctcttagca gcatatattc cagctatatt tttaattttt
     4801 tcttcttgcg ataatccttt caactcttct tcaggtgaaa tgtatttttc aacttcaaat
     4861 ccttttgctg acaaaatctg tttcaataaa tcaatggctt gaggtctatt tccagcaact
     4921 acttcaccaa acccaccttt taccccatgt acatctacta gtaaaatcct tttatatttt
     4981 tctggttcaa taggccaaat atcttgttta tctttaacta atgtaattgc ttggtcagcc
     5041 acctctttaa aaattgattt attttcaatt aaaccaattt tttccagagc ttcttgctta
     5101 ggtaataaaa tttgttcttt tgcttttttg tgtaatccca atttagcttt taaccctaaa
     5161 attcttttta aggcatctaa taaccgatca tcagagataa ttcctttacg ataaccttcc
     5221 atcatataat taaagtcttc atctggatca ttaaaaaata gaaatagatc acatccagct
     5281 gcaatagaag tgggcaataa ttctgatcgt ttcattgccc cagtcattcc taacatatga
     5341 ctagcatcag taactactaa tccattaaat ccgagttttc ctctaagtaa gtcagtgatt
     5401 aattcttttg ataaagttgc aggaagtaaa tcttcatctt tcagatcagg attaaaatgt
     5461 tttgaataag ctggcatatg gatatgacct gccataatcg aaggtaatcc agcttctatt
     5521 aaacctgagt atacttttcc aaaagaatta tcccattctt ccgtacctaa actattaata
     5581 ctaaaagata gatgctgatc tcgttcgtca ataccgtcac cagggaaatg tttagctgca
     5641 ggcaaaattc cactctcctg aatacctttc atataagcta aactaagttc taaaacttta
     5701 tcagggtttt gtccaaaaga tcgactagaa atgataggat ttctccaatt tctattaata
     5761 tctacaatag gagcaaaact ccagttacat ccaatagccg atgcttcaat cccagaaact
     5821 ctccctaatt cataagcata tttcgcatca tttgttgcac caatttttac ttctacacca
     5881 acttcagtac catctgtaca agctccgtta ccacctgctt ccgtattagc agcaattaat
     5941 aaaggaattt tgctttctgt ttgtaggata tagttttgat catatacctc atcagcagtt
     6001 cctgggttat atcgtacagc tccaatatgg tactgattga ccatattact caagtaattt
     6061 ttatcacgac tagatcccat attaacaaac aattgaccta ttttttcttc caaagtcata
     6121 gaatcaatcg tctcttcaat ccaagaaata gcatcttcat ctaaattata tggttttttt
     6181 cgtaagtcta cttttatagt cattttcttt ctcctctact tattagtaag atttttccaa
     6241 aaagtatcta taaatttttg ttcaatatct acatactctc ccgataaaca tttaaataaa
     6301 acatcagttc ttaagttttc ccatgattct ttttctttat taaccaaaat aggataaaag
     6361 aaaactttat ttttatttgc agcttctaga tcaccaggag aatcaccaat cattaatatc
     6421 ttatccaaat cacaaccatt ttcaattaca cttcgaataa tgtcttcttt ttttccttta
     6481 tcttgacaac acagttcatc aactaatgat aataattcat gtctttccca ttcttcttga
     6541 acagcttcct tattagctga gcttacaata attatcttcc catatttttt cagagctttt
     6601 atagtatctc tagcacttaa aaagggttta tctaatcctt tagcttgact aatctgttga
     6661 ttgacttctt ccgaccattt taaagctttt aataaatcct ctgactgata atattctatt
     6721 tccctcttca aagatggatt agacaattct tttgttagag atacccagtg ctttaatcga
     6781 ttaatggatg ttacatcatt tttttctaat gttttaacta aacctaaaaa tctgtttatt
     6841 ccacgagtct ctgaatataa attaatatta ttccaatcct gtaggaattt ttctctatga
     6901 tgaacatcaa aaatctctgc agcaattggt ccgaaaaaga gattgtgttt ataagtcatc
     6961 gtatccataa cacagccatc tgaatcaata caaaatatcc aattattcat atttctctcc
     7021 tatttatatt tttgttttaa aagctcgcaa ttttctaaga ctttatcttt agataatgga
     7081 taaaggaaaa ataaaattaa tcctacaata aaaaaacaaa ttgatggaat taatgttgaa
     7141 gcgttatata acttttctaa aacatctccc gtttgtatat ttgctaaaga gtcatagcct
     7201 ataatagaaa gagtccaacc tccgacgcct ccagctagag cttgacctat ttttctagca
     7261 aaagaatata ccgcatatac ggtcccatct tctctttgat cagactttac ttcttgatca
     7321 tcaataacat cagtaatatt tgcccaaata acagtattaa acacacccaa acccatatag
     7381 ccaaccgaac taagaattaa gaatacatac atattactag tttttatcaa aaataagatc
     7441 aaatatacta ttgctgaaaa gaacattcct actgatgcca gttctttttt tccatatttt
     7501 ttacttaaaa acaaagttaa aggtgaaaca actattaaaa ccaaaatagg attgataaaa
     7561 ttcattaaaa ctattccctg tgcatcatta tagtaatcag gaaatacata attattcata
     7621 ctcataatta ataattgagc aaggagtaag aaaatagccg caccaattat agaaagtaat
     7681 gccctatttt taactaaccc ttttaaagat tttaacaatg aaactttgaa ttgagattgg
     7741 tcaattctta ctcgttcagt agttagttta taacatatta aataacaaat aagtgcacat
     7801 atcgaaaata ttcccgcaac aattgtaaaa gttgatccac cacgaactat ttgagcgcct
     7861 gaatcatctc ttgtataaat aaataatggt gttaatacac caatgatcaa acttgctaaa
     7921 gtagctccaa catttcggaa tgtcgatagc attgttcttt cttctgtaat agaagtaata
     7981 gctgatgcca ttgatccata aggaatattt atagatgtgt aaaaaatact cccccataat
     8041 atatacgtaa catacatgta tactacctta acgctcatag aagctgaaat catagaagtt
     8101 tgatacatta aaaaacttgc taaagcgact ggaccactca ttcttaaaat ccacggttta
     8161 aacttaccct gcttagttgc tttcgatcta tcaacaatac gccccatagt tacatcagta
     8221 actgcatcga taaatcttgc tactaaaaat aatgttccaa cggcagctcc gcttatcccc
     8281 aatacttttg tataaaagat cattaaaaaa gaactagcaa aaatgaaagt aaaatcgttt
     8341 ccaaaatctc caaacatata tcctattttg tctcttatac caaattttct ttccatacaa
     8401 ttctctccct tcttttgtta agcgttttca ttcttatgaa agtacgtact atactctgaa
     8461 gcgtaaagag tcagtattta ttttttacat ttcagagtat tatttgtttt ttctcctaat
     8521 aattctttat ctcataaaac ttatctatat atttactagc tactcgactt acttcttcaa
     8581 aatcaccttt ttcaacagaa gccaataaat tcccacctac accaactgca acaactccta
     8641 cttcaaacca atcttctaag ttctctagac ttactccgcc agtaggcatt atatttacgt
     8701 gtggtaatgg agcttgatag gcttttacaa tggatggacc aaaagcagaa cctgggaata
     8761 gtttaatgat atctacgcca gcagtaagtg cctcctttat ctcagtaatt gtcatacaac
     8821 ctggtaaata aggaatttga tataaattac aaatctcagc tgtttgtcga tcaaaagatg
     8881 gactaacgat atattctgcc ccagccataa ttgcgatacg agcagtaaac gcatctaaaa
     8941 ccgtaccggc tccgattatt acatcttttt gatcagcata ttttttagca atttctttga
     9001 ttatttcttc agcattagga actgtaaaag ttagttcaat ccctttaatt ccacccgcaa
     9061 taattgaatt tattgcttta atggcctcat ccttattttt cccacgtaca accgcaatca
     9121 ctccagccgc ttctaactta cttaaaattt ctactttttt catatctatc tcctctatct
     9181 ccttctttta tggatacata ctttgtctag ctgtactaac ttaaaaagaa taaatttaag
     9241 cttttatttc aagtattatt ttctacttcc gatttcaaaa aaattaaaat aattatacgc
     9301 attacgataa caaatattct ccactatctg actaagatac atttcatcat ctggaattcg
     9361 gcctgcttta acccactcgc ttagtaattg acataatact cgacgaaaat actcatggcg
     9421 aggatagctt agaaaacttc gagaatcagt caacatacct gtaaaattcg acaacaaact
     9481 ttgttgcgcc atcgtagtca actgttgacg cattccttca gctgtatcat tgaaccacca
     9541 agcacatccc aattggattt tttgaatcat acccccttgg aagctttgca ttgctgttgc
     9601 tagtggaagc caatcagttg gatttaatga atataaaatt gtttttggta agcacccact
     9661 cttttcagcg gtatcgaaca atgctacaac attttgtgtc aaactagctt gatcccccat
     9721 cgaatcacca ccgacatcta ctccaatttg ttcctttaat atacttgaat tatttcttaa
     9781 agcatttaaa tgcatttgca tcgtccaacc atatcttgta tactcagaca tcaaatgtaa
     9841 ttgaatacct gtaacaaatt gtgcaatttc tgataaatgg aaatccgttt ttcctgctaa
     9901 agcttttatt aacagattgt cgagtatttt ttcatctgct atttcataaa aataagtatt
     9961 caaaccgtga tctgctaatc gacagccaat ttgatgaaaa tgatccactc gttggctcaa
    10021 tgcttgcttc aacccattaa agtctttgat tggtatagcc gctgcttgac taagttttaa
    10081 aatatagttc gaaaagcctg atgatttgat actccagacc ttatctggac ggaaagtagg
    10141 taataccttg aaagaattat cctgtttaga aagctcagta tgatacccca agtcatctgt
    10201 aggatcatcc gttgtacaga tggctttcac atccatcatc tccaacaatt ctttcgggcg
    10261 ataggaatcc gtttggattt tttcgttagc acgtcgccaa atttctggtg cattcttctc
    10321 agaaattgtc aaatcaatat caaagtaacg ttgtaattcc aaatgagacc attcaaaaat
    10381 aggattacct ggcgccttct ccactgtttt tacaaaagct aaaaattttt cataatcatc
    10441 acctggacca ctgatcaact cttcttctat accattagct cgcatcaatc gccatttata
    10501 atgatctcca gctcctttat catttaacca tacctgagtc aaattcttgt attgcttatt
    10561 ttctagcaaa tatgctgggt ctaaatgaca atgatagtcg ataattggtt ggtgttttgc
    10621 aaatttatga aaaagtcttt tagctatttc attctctaat aaaaagttct tatctaaaaa
    10681 catatttaaa cactcctttc cttccgttag tttttagaag agaaaatttt tcctctaaat
    10741 aaactccatt atataattat ttactcgccc ccttttcaag cgcttcccaa ataccattta
    10801 aataaactgc acctagagct cggtcatata atccataacc aggtctaccg gtttctcccc
    10861 aaatcatccg accatgatct ggacgaatat agccattaaa tccattatcg tacattgttt
    10921 ttaatatctt atacatatct agagaaccat attctgattt atgcggcgct tcgtaaaaat
    10981 cgagtccatt gtcacttaaa tatttgatat tacgaacatg tgcaaaagga atccgatccc
    11041 gttgacaaaa ttcagataga atttcataga catcattatc tggtcgttct gcaatacttc
    11101 cagtacataa tgtaatacca ttagaaggag aatccactac attacacaac caatctaaat
    11161 cgtcacgatt tttaacaata cgaggtaatc cgaaaatgga atatggtgga tcatctgggt
    11221 gaactgccat tttaatccca acttcttcac atgtcgggat aattgctttg agaaaatatg
    11281 caaagttctc tcgtaatttc tcttcatcaa cttctttata ggcttctaat aattgcttaa
    11341 tttgtgataa ccgatgaggt tcccaacccg gcaattcaaa attattagta gaattttcta
    11401 cttttctaat aatttcctcc ggatcagtag ggatattttt tgaaatatag gctaacgtag
    11461 aagacccatc atctaaacga taatccaaat ccgattttac ccaatcaaat actggcataa
    11521 aattataaca gataacctca atcccaaatt ctgccaaatt gcgaatcgtc tgtttataat
    11581 tctcaatgta ttgatctcgt gtttgtaaac caattttgat atcatcatga acattaacac
    11641 tctcaataac tttcaaggta agtccattgg cttcgacttc atcttttaaa gatttaattc
    11701 gttctctcgg ccaaacctct ccggctggta tatccattaa tgttccaaca attcctttca
    11761 ttgctgggat ctgacgaatt tcttgtagtg agattttttc ttcatttgcc ccataccatc
    11821 taaaagtcat ttccatagtt actttcctcc aacttctgtt atatatttat tgattgtttt
    11881 acgaactgct tgaggaccta ctagcatttc gcaaaagatt ttttcagttt tctttcctac
    11941 cccaacttga tataaatcta aaccaaaaat ttctttattt tctaaaattt cctgtaccgc
    12001 acaatgaata tttgaaggat cttgttcacc taatttcaat actgataatt tatcctgcaa
    12061 ctctaccaat aacggatcag gacttggcac aaaattttcc ccttgatcat cgactgccag
    12121 taaataacgt aaccaagcag caatagcgat aggaataaat gttagatttt ctgcatttcc
    12181 gtctttttcg acatactttt taattgtttc accatagcga atcgctactt tttgagaagt
    12241 atctgttgca attcgttgtg gtgtatctgg aatataggga ttaggcaaac gtttattaat
    12301 tacttcatca ataaattgtt ttggatcaat aatgcctgga ttttcaacta ctggtaaacc
    12361 ttctaaataa cctattcctt tgattaaacc aaccaaatca tcatcctgca tctctgctga
    12421 aattgattga tatcctagca gacaacctgt tactgccaaa gcagtatgaa gtggattcaa
    12481 gcttgtcgta accttcatga tgtctgcttt atcaaccgtc tctctatctg tcataatcac
    12541 accagccttt tccaatttag gccggccatt tggaaaacta tcttcaataa ctaaataatg
    12601 aactacctca gtattagtga aagcagcaat attggtatgt ttttttgtgt gaatgatatc
    12661 taaatcctcg agccccatca tcaatagatc ttcctttaca ttctctgaag gatttggcgt
    12721 aattcgatca atcatactcc aaggaaaagt cactttttct ttatccttta aataagcaat
    12781 aaatttttca gaaacatatc cttctttttg ccaagcttct gcaatcgtaa aaatactatc
    12841 ttgaaaacgt tgaccattct gtgagaaatt atcagtagaa accatcgcaa tcggcaactc
    12901 tccagcatta aatctgctat ataacaagga tgttacaata ctcatcgtat gttgaggatt
    12961 gctaggtcca gatttgatat cagctttgac taccgaaaag aattgccctt tagaatcttt
    13021 taaactgtaa cctttttcag taatagtaaa cgttgtaaac tgtaatttag gatcttcaaa
    13081 atatttaacc actctttgat agtccgctat attcttcgga ttgcaatata aactatcagc
    13141 cgttactgcc aaaagccttt ttttgagttc accatctcca ctcataacaa tttccaaaat
    13201 gtcattgttg tagtcatgat aagctttttc cactacttct tcatcaaatg tttcacacac
    13261 aactactcct gtttctaatt cattttgatc tgctaagcat tgagcaattt ctgcatgaaa
    13321 tcctcgataa agatttcctc caccaaaatg aagccatact ggatgttgct ggccttcttg
    13381 cttaacttct ttgacatcaa aacacggcag tagaatctgc atttcattga attcttcgat
    13441 agaattttgc caatttttaa tcgaaaccat aattaacctc cttaaactaa tatgttagtt
    13501 ttgatacatt ttatatttta aaacccgttg taaccgttgt caatataaaa atggaataaa
    13561 cttgcttttt ttctaccata tcagaattat tgtatttttt attcagaaaa ataaaatact
    13621 ccttcttttt ggactgattt tatcgaaact tatttttttc tttttttcat gtattctttt
    13681 atttttataa ctagtgtatt acttcaaaat ttgatattat atggcatgaa cagaattaaa
    13741 atatcagcta aaataaaagt taaggagcat tgagtatgac aattaaaact agtctccaaa
    13801 atcaagctta ccaatctatt cgccgacaaa ttatctatgc tgacttagaa cctggaaaaa
    13861 aagtatcaga aaaaaaatta gaagaaacac tttctattgg tcgtacacca atacgtgaag
    13921 cactgattca actccgacaa catggtcttg ttgatacaat tcctcaaagc ggaaattata
    13981 tctcattaat tgatttagat tcggccaaaa atgctcgatt tgtcagagaa catttggaac
    14041 gacaaattat gttagagtgt tgtgccaaaa ttaataacca aaaaaaacgg attttagaaa
    14101 ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt ttttttcata
    14161 atgataattt attccataaa gcttgttttg aaattgcaga gcgagaagaa gtatggcaat
    14221 ggttagaaaa taacaataca cacttagaac gatttcgttg gctacgagta aaaacggaag
    14281 gattaaagtg ggaaaaaatt atggaacaac attatcaact atttgaggca ttaacaaaac
    14341 aagatccaga agaagctaac tttctaacat cgttgcatct tcatttaatg cttaatgagc
    14401 aaaatattgt tttagctact catccaaaat atttcaaaag tatataactt ttaactcttt
    14461 ttaaattaaa tgattgttct gatcagaaaa atatcgcttg ttttcttatt atgtaatata
    14521 tttcataaac tattctgcat aacaactatt gtttgtactt tttacattgg ataactaatt
    14581 atgactgctg ataacttttg atattaaact agtatctcca atccaaataa aaaatagata
    14641 tcttgttcat caaaattagt tcatggatac ttaccttcat ttacatcatt cagcggtttc
    14701 tcattttctt ttattctatt atttcatttt ctaatagact tggcttcatt tcgcaatact
    14761 tttttacaat aggcatcaaa ttggtgttgt ttagatattt agttgtagtt ttgtgacatg
    14821 ataagtatgc tcctttttgt cttttccact aatacaacga tcaaagggtg cttttttcaa
    14881 aaaactagaa aaatcaaaat aagattcgta ttttgattct atatatcctg tttcaactac
    14941 atcttatatt tcataataga ataaatagcc ttttgcatag ctgcttaact tataagaaat
    15001 tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa ataaaattat tcttcttaca
    15061 gataattaaa aatagcgcgt aacaaaaatc ggcattcaat cacataatta ggattgatgc
    15121 cgattactta tattctacgt atagttttag tattcttttt ttatcaccag taaccaattg
    15181 agaacagtcc taaagcagaa tagtctttta tctcaaaacg cttaatttca ggcttaggaa
    15241 tatgaataat cttttgagta tattctttct cttttaatct tactgaagaa ataccactgc
    15301 ctatcttttt tacaaatatt tcatttggtg tacagactaa taaaacaata tttgtaatca
    15361 atccttgcac taaagaatta aaaggaagac tatttaaaac agggtgtgca taactataac
    15421 atgtgaagta gttattttga actttaactt ttaaaaactt taaaaataat cttatttgtt
    15481 cttctaaaca atcatctata ctcatataat cactccacta attacttccc ccaacaagaa
    15541 atagcattaa tagctatacc agctgcaata gttgcagcat tgaattttgc aaattgtgca
    15601 ggagttaaac ttctttttgc tacatctaaa agtattttaa cagcataatc ataatttttc
    15661 ccttgcaacg cattaattaa agtttgcatt agattttcac gattaagtat atcccaaaca
    15721 acacaccaaa tggtaatgag ttcataacaa cacatgcagc atacttttga agatcacgtt
    15781 tttgacgctt tcctgttggc tgcacaaaat tttctaaaat aaatttccct gctatacctt
    15841 cagagccatc taattcagct tttttctgta tagcttctaa atcagtaact atatattgat
    15901 gtgtttctgt atcaacataa ccagcttctt ctaaatagaa tttcaaactc tctactgcct
    15961 gttgtctttg tgtttctata tcaggaactt cattcacttt ttcagcagca taaacactgg
    16021 ttccggcacc gactaacgta cttaatccaa ttaacattac acctaataat gaaaaaaact
    16081 taaaaaaacg actcattttt atccctcctg aaaagtattg ttgattatca atttttatcc
    16141 ttttaagctg ttcaataaga cgtttttacg ttccttccgt ttctatttta ttctttctgt
    16201 cttataacag cttttttcaa gtaaagaacg atataattct tcttaaaaaa cagtctattt
    16261 tttcggtata taataaaatt gtattgttac ctacatagcg aaacagtacc aacatcattc
    16321 aaatgaagat agcaaatttg cttcttcctt tttcttaagt gactatttaa ataaccagaa
    16381 aaaaactaaa atcacactga tatcccaaat aaattgaaat aaaggaaaaa gtttttttct
    16441 accatgatag tttttcaaaa aatagaaact cgtactattt aaaagtaaaa taactagcat
    16501 cgtaaagact actgatttat ctaaacttct ataaaaaagt gggctaaata ataaagtaat
    16561 taaaaaatga actatttgat attgtttttt ttcatattaa cagatcgcag ctcctgcgat
    16621 tccataggct gttcctacag caaatggaat aacggctgca cctactcccg taaggcttaa
    16681 acctagagca gcagctgtat aaatcccaga atgtagaagt ccagccccag tgaaagccca
    16741 actgcaggca cttcgttcac cagttttatt atttgttttt tgatctttca tataattctc
    16801 aacttcatga agctgatcgg ttagccatcc atggccattt tttttgaata gaacgattaa
    16861 attcatttag atactgttgg atttcagaaa ctgtaatttc agaattaatt ctatctaaac
    16921 ttgattttaa atcactctca tcaataatat atactaatcg atttccttta gaatctttat
    16981 gtgtggctaa attattttga atgatattta tttcagtttc ttgttgtttt actaaaaaat
    17041 tatttgtttc agaagcatcg acacttattc cagcgctaac aaaagtactt aaacctatta
    17101 agaaaacacc taaaatacca aatattttaa ctattctttt cataattttt cctacttaaa
    17161 aataagttat ttttaaaata tcttgattat gaattcatcc cccaaaggcc ttttaagatt
    17221 gatacagcag tccgttcact tcttttagac ttgtcatata agtcttttaa atgatcccat
    17281 accagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg gttcattttt
    17341 tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc atcttggatt
    17401 tcaataatag tatcatcaat tagtttttga atttctttcc tatcaacata ttcaatctta
    17461 gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc ttcagacatt
    17521 ctcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg aaccagtaat
    17581 tacactatct acaacgccat tccaatttac tctacatttt ccatttttca gatttgtcgg
    17641 tccatacgga tagacttttt gacattcaat gttcggatta taccagatag gattatacca
    17701 ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa aaccaataca
    17761 catcacacta tagccaatta attttttcat ctcgtatcca tctcctaaaa aatagaatag
    17821 ttcttgtagt tattcataag acatattttt tccgtttttg ttttatcctt tctgtcctat
    17881 aacaactttt ttcaagtaaa gaatgacata cttcttctta aaaaaacagt ctgttttttt
    17941 ctattttttc ggtgcataat aaatatgagt gttcattttt tattttaata cacacttcgt
    18001 acataataaa tcttgatcgc atcaatcttt tgagcttctt gatctacttt caattgaagt
    18061 aatacaaatt gagtgacctc attcgttgtt tcttctccaa ataacaaata ttgatccgga
    18121 ttctctatat cttgtgtcac ctggtgaagt ttcccagttg ttttcatttg tacatgcggg
    18181 tctacatcaa tactattgtc tttaatacaa cgatccgtca tatagtccct cacagcttga
    18241 ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt taagaaattt
    18301 tttgtcattt catctatttg ttgcgctgtc tcgtcttcta atgaaccaat ttgactgtta
    18361 ctttcagtac tttgaagagt tccacttgtt tcttttactg cttcgattgt ttcttctgtc
    18421 gttgtcaatt gtgttttttg tttttcagaa gaaaaccaat gacaaccagt aagacctatc
    18481 aaacataacc aactaaatac taaaattatt ccttttttca cgcttctaga cactgccttt
    18541 cttatttcac cttacgaacc agactacgaa tttttgtttg attgtacgtg cgttgatacc
    18601 gagccataat ccgtccttgc tcggcattct gttccacact agagaaatgt tgtccgccat
    18661 ctgaaacatc tgttataatt ccggtgtgac catagattcc aggagacaat gcaccacctg
    18721 cttgccaatt caccacatct cctgcttgta aatcacttgg ttttgggttc atgatcactt
    18781 gccaaccata ggctgtccaa tcataatctt cccctattaa catcgcaaac atgtgtccag
    18841 aacccattaa ctgtgggcca cctagttggg atacatacca cgccgtgcca ccataacact
    18901 ctccattatt taccgatcgt ccaatgatcc gatctaaaac tttcgcatta cctttacttt
    18961 tccctgcaac aagtgacgat ttttttgttg gtaggctatt ccaatgtttg atcgcaaaag
    19021 tatcatattc tgttaagtta taagacttta tgatactcat cgctccttgg taatacggac
    19081 tgccttcggt aatacctgcc gccgcatagc ctccacgacc tagaatcaat aaacaactcc
    19141 caggatcttt attgcccaca cattgattaa agttgctttg actcgccacc aaaaagccgt
    19201 aatagtcata acaatctttc aggttcggaa acttcatata gaagccacct tcagaaccac
    19261 caatcccccg tgcagaacct tgtggatagg gacaaccagg aaaccaggta atcccaaaga
    19321 agtttgtatc gtttcttcca gattgtgatt gtccataagt cgattcataa cctaattgag
    19381 aaagaatcac acttggtaat aactgatact ttaccgctgc accgactgct gcttgcgcat
    19441 gttcatctgt gatcggacca cgtcctttaa tctctgaaaa aatcggtaat ccttcggtat
    19501 attcccaatt aaaagcgaca gctagacctg tagcctctcc tccatcctcg gaaagttctc
    19561 ctgaaaaaaa gaatggcaat agaaaaaagg gaagaataaa gaaaagcaga caacctagac
    19621 accctttctt catttcttcc tcatcctttc ttcgataatg gtttttgaac tgttcgtcct
    19681 tttggtaaag taaaatcggt tcctggtttt gcgtccaaaa tcggcaatgt ttcatttttc
    19741 ttttctattt ttttcgattg ctctactgga ttattaaaag aagaaaatgc aggttgtaag
    19801 gattttggta cggaaatcga cggtttactt tcagacgaac gttgtatctg tcgttgcaca
    19861 gaacgatttc ctgatagagt ggctaccgac gacttaggaa cagagtattg attcaataac
    19921 tgctgcattg ctggataact cccatttttc gcctgatttt tcacttggtg aatctgttgt
    19981 gaaagagaac cagcagctgt taattgtggc acgggtactt ttccagcagg caacggaatc
    20041 gattgaccaa caaaagcgat acctttattt aacaatggtt ttctttgatt attcccttgt
    20101 tgcttactcg attgtgccaa cgattgtacc tgttgtttcg gtcttactga attcatggac
    20161 gaatttggaa tcaaaccatt tgaaagacca gaattccact ctgtttttga tttggtagaa
    20221 gacatttcag gctctacccc atgataacga acatgacgac cactataagg caagtcttga
    20281 tttgaacgcc cacctagttc aaagggatta atcacttgcc ccccttttac cgattcttgt
    20341 ggtgtacgtt tttgttgaaa cttttgaaat ccatgtatcg ctgcagaagc gagtggattc
    20401 gttgctcggt acacattccc tgctaatcct gcacctttga ttgccaaatt ttgcccagca
    20461 gttgatagtt gaccaggcag ttgtttcatt tgagacaaag aatgacttgc tatgtttttc
    20521 gtgttattta tggtttgatc tgcagctcct aaccctagtt tctttaataa atctttccgt
    20581 tttacaatga gtaaaattga taggagtgct ttgacaaaca tatttcctaa aaaaccaaaa
    20641 acagtattca ttagaattaa atcgtctact atgttataga tcaaaaatgc caccaaaata
    20701 atcacactgt aaaaagattt ttgaaacatc aagctcaaaa aggactttaa ggtattcatg
    20761 atcatataat caaatgctgg aacaaaagaa gcgagtaatg aaaaaggaat cacaagtaat
    20821 aaacacaaca ccaacatttg aaagataaaa cgaatcatgc caatcactac aagtggtaat
    20881 ccaatactta gattaagcaa tggcaccata gaaccaacga ctgctttata aacagcttta
    20941 gttggttgaa tatagacacg ataagccttc tttttccctc tatcttctcc tgcagcagtg
    21001 gtcacttttg tctcaatatc agataattct tcacttgacg cttccttggc tagaaattct
    21061 tgtggttcaa tctctgcttg atacaattct tctaaatttg ttgtcccata atttaacaat
    21121 agatacggat caacaacagc cgctttataa taaagttggc gcactttttg aatggcttcc
    21181 ttttgtgtcg ttggcactaa aacaccattg acatcttttg tcgccaattc atctagacta
    21241 tttgtagcag aaaaaataaa gccttctacc tccacggaca aatcatttaa ccctttagta
    21301 aaagcttctc ctttactagg tacgttacta ccatagccaa accaaaccat tgaaaaaatg
    21361 acaacaaaga gaaatcgaat aaacaccatt tttgcgtaat gtttccctct aaatatacga
    21421 agagtaaata gatataacac atacaagacg acaatccccg caccaatagg atcgtatatt
    21481 ttcgaataaa tagtatccga aaaagtgaaa aactggtgaa ttaactcgtt cattgcagct
    21541 ccttcataga gcttctcaat gacaaaatca aggccttgcc aaatcaattt acttaaacta
    21601 aagaacatat tcgtcatcat ttttagaaaa tctgtgccat ttgctccaaa taacattttg
    21661 cctaatgaaa attcactatc agataaataa gaagaaaagg tatcaatatg aaatttcccg
    21721 atctcataga tcgattgatc cacctgtttt tctaccgcat ataccaatcc accaacaaaa
    21781 aacagtccga atccaaccgt taaaaaaagc caaacgcacc atttcttttc cttacgcttt
    21841 atataagcca ttgtttcacc tcgtttctat tgcatgatta acgtatcctg ttcttctatt
    21901 tgagagtgac ttgttttttc cgttgtttgt aatgcagcgg tcatttcttc aaacaaactg
    21961 tgtacattga gttttcctac acgtccatag ggatctaaca tccaacactg tcctttaatc
    22021 atatggctca tttcagataa gttcgcttca tttacttcca aacgtaaatg agtcaaaatc
    22081 ttttctcgct cactgtcttc atcaaaagcg aagacaacac caaaattccc tttttcttta
    22141 tctgaaccta agtcgccaat aaattgtgta tctagcacta attgattcat ctgtgaacgc
    22201 cccacacgcg ccattgctgc aataatacta cgtcccgcgt tcgaattatt aaatacccat
    22261 gcttctgtaa aataaatcgc tgttttctta ctactatctc gttttccaaa catttcacaa
    22321 aaacgaccta aactaatcat caaacacaaa gactttcgat ccgcatctgt atatagttct
    22381 ggtcggacat tgtcatttgg taatttcaaa tcttggatct ctaaaatcgt tgttttggca
    22441 ttaaagtcta atcccgggtt ttctccataa gaaaatccta aacgtaaaat cgaatcttct
    22501 accgtcaagc gtaaaaactc accatattct cgaacctctt tttgttccat ttgttctaaa
    22561 tactcgacca catgcatcaa gccaacttgt tgtccggctt ctttttgaac taaaacttct
    22621 tggattccct ttaataccgc cccttttaaa aggtgattgg tacctaacgg acttaactca
    22681 tcaatcatat cctgggctac ttgtttcgca tctactcggg ataaacaaac aattggatct
    22741 aatacgccat aattacgggg atttgtagca tctaacgtga cataactcat tttttgcaaa
    22801 tgttccacaa acaaagggta atgtttctga taatacgggt cttgaatgag tttcgtaaac
    22861 catttgcgct tttctccttt gggatccaca aataaacttt gtacattcat aaacgaacta
    22921 tacaaaaaga gcaatccaac taaaaaggtc ttccctttac cggtttttcc agttactgct
    22981 acatgaggcg catcaaacaa cgcacctttg attccttccg caatagctag aagattcaaa
    23041 aagacaaaac gatttgacgc ataaattaat tcttctaatg gtgttttttc tcctcgagaa
    23101 ccaccatccc ctaatgtatc ttgccgacca atataccagc cggattccat acctaattct
    23161 gatactgctg aaaacaacca ttcgccaatt gcttctacag ttgttcggtg cttccaatgc
    23221 aactgtcgac ccaatggttg ccctaataaa catttataga acaattctcg ttgatcattt
    23281 tttgcacggt ggatatggat agtcgtatga agctgttgaa taatcgttgc actattttga
    23341 cgaacttctt caaccgtttg accgtataca ataaaacatg cgatccaatc gacgacaggt
    23401 tggccattat ctactcgttg ctttaaatgt tttactaatc cccgaacagt taataagcgt
    23461 tcgctaggtt cttcatcgtt ttgaaccaat tccttatatt ctgttttgaa ccgttccgat
    23521 agccaattta gttttaaacc aatacctccc tgtcctttca agcgtggata gtgaattttc
    23581 attcgtaatt ctgctgggaa agaaagatcc tgtgcaaatt gaaatagatc acagtttctc
    23641 atattttcag gtgtttctcc aataggtaac aacgctaaat aactagtgcc caactcatct
    23701 tctaattgaa gaaggccttt atgttccttc ggcatacgta catacgttcc ttgattgtct
    23761 ttcagtactt cccaattatt ttgttcttct actttatgag ccattcctcg aatatattgc
    23821 agacgattga gatacaacag ctcttttttt actaagggac gaatagaaaa gctataaagt
    23881 aattgtgtca cttcttttac ataaggatca ctttcagaaa acaaagtttc taaatcatct
    23941 acttctaatc caaataagcg tgctaaactt cctgctacat ctaaccagat tagatttagt
    24001 acatcataaa aactctccac ggttgctttt tctttgactt tgattcctaa aataaaggaa
    24061 ggacgataga cttgaccttc tcgttccaac aatgtcactg tttcttttgc ataatacgca
    24121 ccaattgctt gactatcggg agaaaatccc tttcctaatg tgtcaaattt cgtccgtaaa
    24181 ttcattgttc gtggatacat caccaactcc acatcttcat aatctgctaa aagtaacagt
    24241 aaacgagcca tcatttcttt tatttcctgt gtcttttttg gtgtgttttt tgaaatggtt
    24301 cgtggaaaaa catgatagta ggcccagata caatcctcgc catttaacaa acaatttttc
    24361 tttaatgctt tcactggttc ttttacaagt accaatagat ctcacctccc aattaaaaaa
    24421 aggaacaacc agttgattgt ccctttttct aatcctctaa ataaacatac ttattcgcaa
    24481 aaagtaattc ttccaatgca ttatttgata ccttgtccca gttataatac acacaatcac
    24541 cattcatcat acgcactaca taacaaaacc aatcccataa ataaaaatgc actttttttc
    24601 catcaggttt tagttttgcc agtgctaaca cacataaata gggtagccct ataaaaaaaa
    24661 caacccctgc tggtggaaaa aaagaaataa cccaaccaat aaatgaacgt aaaaataaat
    24721 attcaaagat tacgaccaat aaaaaagttg ctgcattgac taatgcaact ggaaatggaa
    24781 gacgccaatt gcttgtaatc tgttgtacgc gataaggctc cgcataaaca atcgaataat
    24841 cgtaaggttc ccaatccact tcactatcct cctaatccat aaataatttt tcaatgattt
    24901 taccaaacgc atcaaataca cgcgttggat catttaagaa gaaatagact gcggcacctg
    24961 ctgcgataga tattaatgca tttcctactt ttgatttcat aaaatttcgc aatcccagat
    25021 aaacaaccac cattattaaa aaatagccac cttgcgttaa tataaaatct acaattccct
    25081 tcagtgtcat ttccgttcgt ctcctttcct atccaaccaa ttatgtgtga gtttttcaac
    25141 ataatactga tttgtttttt tcgtaataac aagcgtcatt tgttcttttt gtaaaagtga
    25201 cgtatcacca tctcgaaaga cgactgttgc ttttacaata aaagtttttt tcgtttcttt
    25261 ataacgcaat actttcatct ctacttgatc aactttagct gtctgattga gtccttcagg
    25321 ttctttcatc atgtatgaca tatcttttgc attgtttttc gcatacttat ctaagaaatc
    25381 ttccacaaat ccttccacat ctttcgcttc cgacgcatca accgtttcgt ataatgtgtc
    25441 atcataagta atcattcgtt gtttttctgt attctttggt agtgccgtaa aataaggata
    25501 agcaataata cgacagccct tgtttgaaat agaaaaggga agattgagta cttgtgtgat
    25561 tgtcacactt gtttctttct tattctctgg atccatcgag cgaatatcat aagttacttt
    25621 ataactagct gttggaatgg tttcaatttc ttttaatcca ataaacgaag cagcagttaa
    25681 ttttcgaata cctgaaacac ttcctggttc ttgaataaag ttttgtgcat aaaactcttt
    25741 ttttaatatt tcttgacgtt gttgttcttg ttcatgatta tcgaatgtat tcatatacaa
    25801 agcaacaaaa cgattcataa aggattcaat ttcaggtgtg tacgttaatt ctgactgact
    25861 ttgtacttct ttcttttgta attgttggat ttcatctttc aaaaacgaca cttgaacctt
    25921 cgttttcatg gattgaaaaa atgccaagcc ccctgaaaaa caaagaaata aaaccaaaca
    25981 ccaaatccat ttcactgctt tttttgaatg gattttcctt tgattcggta ctttctctgt
    26041 ataaactttt tctttccgcc gtagttgtcg ttttttcata agctactcct tctagtatgt
    26101 ttttactagt acgtacgatt gacaacgata agcttgtttt tctgaaaaat agtccattca
    26161 attaccctca ttttctattc cttttctctt tcttttatat ccaacaaatc cagcaatact
    26221 tgtcaacact cctcctagaa cagaaagtac aacatgtatt tcttcaccag tctttggtaa
    26281 gacttttgtt tttccatctg ctgttttgac ttcaatattg ccacttggaa gtttctttac
    26341 aggaatcgac aaatccttta ccaaattccc ttcactatct tggattaatg gaattccatc
    26401 ttttacacca acaattgttt gtccaccttc tgcttttagt ggagttgttg gtgttggaat
    26461 atgggtcaat tcctctacat tactagtgac aatcggtaca ctcattcctt gagaattatt
    26521 cgtcgtaatt tcaccgtttg gggttaccga tactgtgact tcttttgctg gaacaatagg
    26581 tttattttct tttggaattg cttctgattt ttcagcaggt tgtttcggtt cttcttttgg
    26641 ttcttcactg gtaaacgaca tcgttggctg ttccgtactt gacgaatcgg tattcgtttc
    26701 agaagaactg gttgttcctg attgttctgg agttgtgact atacttgaat ccgttgttgt
    26761 tgaactgtgc acaccttctt cttcaattgg atcagtcggc aaagttgatt caggaatctc
    26821 tggttctggt gccactggct ctttttcagg tttctctggc tgcaaatgtt caggttctgc
    26881 tggtttttcc gttgttcctc catttagctc tgtctcaaca tctgataatc cttcccctgg
    26941 caatgtttct acaatttctg acttaggtgt ttctacagtt tcatctgcat aaaccaatga
    27001 actccctaat tgaaacgaaa ataatgaaat tcctagtgtt gttacgactg ctaatgttgt
    27061 ttttttcatg attattcctc caatttttta tgcaattcgt gttttgttca tagacgccgc
    27121 actaatatta tctgaacgat aaaagaactc ccgtccgttt tcttctaacc caacatatgg
    27181 attatttttg aataattgaa tcaatagttc actggtgttg tattttggat agtttgcaaa
    27241 aaaataatac tgtgcttgtc gcccatccga aaattcaaca agtaattcat aacgataccg
    27301 cttcaatcct tttcgttgtt ttaaaggatc tctataccaa cttgtccatc gttccgttcg
    27361 tggaccgaga tagacacgat acacatagaa caatgccata ccacatacca ccaaaaataa
    27421 taatcgccac ataaaaattc ccccttttct tctacctttc ttgaatcacc gaacattcat
    27481 accctcggta gagatccgaa aaatccatca ttaatttttc gtataaacgt tgttgcaaat
    27541 agcgattgtt tcgaatacat aattcttcat agccatcaat cacttgatct tctttctcat
    27601 agcatcttgt agctctatga cgctcagctt gcatgacata accaaactct gcaccatatc
    27661 gttctaccgt taataactgt tgactacgca ataatgcaat ataacaatca caaaaatgtt
    27721 cagccgacat ttgatttttc tcacgtttgt gaaataggta ttctttagaa agcacattat
    27781 tcgttaaaca atctgataaa tagcgataat ctggcggtcg gttattttca tctgtgaatt
    27841 tttcccataa taaactgatt tttattcgtt cagcggctag atagtcttgt tcgcgtggat
    27901 aaaattcatc aatcgtacaa agctttttta tcacccgaat aaacaaatcc atataatgcg
    27961 aaacgaactc ttccttttca attggctctc ctaaaaattc cagtgacata ccatcttctc
    28021 cttccttaaa aaataaaaaa gcagaaaccg atttggcttc tacttttctt tccttttata
    28081 gctctttttt acggaagaat ccgcaaatga cattttttca aaaaattaat cggcacaagt
    28141 tcacgaaaac ttggagcaat tttagataga atcacttccc catcttgtaa aatacaatca
    28201 taaaaatcat cagttacttg atacttacgc ttcttacaat aattgataaa atagtagcga
    28261 tcattttcta tttccattcg ttgttctact aagcgcatac gaacatcatc atagaaataa
    28321 gccacaaatc gcccttcaag ttcacgtttt tcaatgggat actcttttgg taacaatcta
    28381 ttttttcgtc gtttatacat tgctggatac cttagaagaa gttcatccaa acgtctgtca
    28441 gtatatggca actgacgaat caatgattcc aatcgttctt tatacagata gaataattgg
    28501 cgcaagcaat agtgtactgc ttctggattt tctaatactg ttggatcacg cgaccaatac
    28561 gcaccaacat aaatcataat ttttttcatt tagacaatca caccagcaat actaatcacc
    28621 gatccacaat aggtagaaca agtggcatag cggccgattt cctctaattc cagctcaatc
    28681 tcttctaaag aaagatacgt ctgttcttct gttggctggc gattcatttc ccaagtaaca
    28741 gttgccaacg agatttcttc cacaagaggt ttttcgttgc taagtgctgc cacacagttt
    28801 tcgtatatct tcattttatc gcagcctttt tttaccgcta aaatcgcagg taccggcaat
    28861 gaaaatcgat aaaagcgaat gaattttagc gttccttttc ttcggctaaa ttccaactct
    28921 tcgagttctt gttcgtaccc cttacgaact tctaataagc gtgcaatttc ttgccagttt
    28981 tctttttcct gataacgtcc agcctttacc gcttgttcaa tataaggaac tcgaaaacga
    29041 aaataagaca aatcaaccat cttttcattt aatgcttcaa ttgcagcctt taaatccaaa
    29101 agacgcagcc attctgttgt cgtatatgca ttcatgcaat tttccctcct gacgttccca
    29161 tccattctag atagctttgt tgctcgtcat ttagttcagc attctcaatt ccttcttgta
    29221 caaagtctct tcctaaaata cgatctgctt cttgtaataa ctttaatgtc ggagctactt
    29281 gtttcaaaaa ccagcgttct gaacgatcta atgagaattt ttctggcttt acggtaaaat
    29341 ccaattcttc catcgaacga aacaattttg cccatcgttc atcaacaatt cgtgcatcac
    29401 tttccagtcg atcccaaaca ataagttttg cattaattaa accaacgcca atctctccta
    29461 aacgtttagg ggaatgatcc aaaataaacg cttctactgc ttgtgttgct ttttcattag
    29521 ctaggcgtaa ctcatatcga ttataaatgc catatttcgt taatgcttct atttctgaaa
    29581 ttttctcacg attcgcaatt tcaaaacgtt tttcgtagaa attgaaaaac aatggactct
    29641 ttcgcgatcc aaaatacaag gataaccctt cgcttcggaa ataaccatca tctaaaaaac
    29701 aaccgccaat attctgaaat ttcttaaagt ccatttctaa taaacctgct ttttgttttt
    29761 ctagtaattc aaagagatca aaatgttctt ctgtttccaa tccccaaagt tcatctaacg
    29821 caatatcgaa tcgactacat tgtactcgtt tataaccagc ttccaacttt ccttgatttt
    29881 tccaaatgga atatagccat gttcgccaat ctgttcgttg ttcacgcatg acttcctcat
    29941 attctcggca accttgaccc gaaaactgta caagtacatt ttgtgttaca gtttgttttg
    30001 aatgccagaa tacccagata tttccacgaa cagaaagaca atcaaaaaga tacaagcccg
    30061 tcggacgtgt ttcaaattct gttagatcca ttcgtaacac acgttgaatc actattttgt
    30121 agtctgtccc aaaaaaggag agacgcaaat agtcgatcat tgctcgaaga tctattccgc
    30181 ttttcatata caatcgaaag cgttccttga ttcgtttttc gaaatcctct gggattttct
    30241 tttttccttg ttccatcttc tttatataat ctaccgagca tcccaataac tgtgcaaaac
    30301 gtttttgaga gagtaatttt ttccctccac ctgaagcgtg aatagcactc aatttttttc
    30361 gataatttcg caattcaata ggtgtcatat tctacctcct ttataaaata acaaatttcg
    30421 attttcttaa tctgctagaa atcccgatat aacaggtggt accccctaat ttttctaaaa
    30481 aggagtactc cttctaacct tagctaaagc cttgatataa ctgatttaaa tacacttttt
    30541 tatttgtgtt atgttatttc tgccccccat attatatatc ggggggcttt acacaaagag
    30601 gccgtgcgca agcgcacgac ctctttgtgg gcacacatgc caagcatgtg ctgccacgac
    30661 tggtctcggt ggcttcgccg acacaagccg cggcagcaca tactcggaca atgtgttctc
    30721 ccatagtccc tatgaaagcc accccgatat ctaaaccaat gaatcttctt tttcttgtaa
    30781 ttctttatac tgttcaggag caaaacactg taaaatcgct aaacgtaact gtatgttttt
    30841 ctctttgcat aaccgatata cctgacgaaa acgcaatata tcctcttttg tcaaaacaat
    30901 ctgatttctc gtaacaaaca atgtctcgcg ctcaattcgt cggaaaacat aagaaccttt
    30961 tgtgtagtat tctactttgt taatccactc gtataatctt cgaaccgcaa aatcaggtga
    31021 aatttcaccc gcagctgaag aaacacgata caaaacttca cccatacttc accacttcct
    31081 tctaaattat aaaagtgtat aactttcctc atctacccca tttgttttcg tatttccttt
    31141 taacgattgc gcttgacgat caatttgttc ttcgtaacta gtaaacgcct cttctttcag
    31201 tacagaaaaa tcttgcacaa tcattgtgtc aaatttttta aattcctcaa taaagtgata
    31261 gtctttgtaa acaaatggcg caaagaattc tacaggcaca tcaaaaccag gaaaagcata
    31321 cccacgacca ggttgatctt tgttataaaa cggcttattc ttaaaatcct ctccaaaagt
    31381 cattaaatag ccaatgggag ataaacggcc aacagaaata cggaacgtta aactatctcg
    31441 aacaccactt ggaaaatatt ccgcatctgg tcgctgcatc gctaaaataa catacatgcc
    31501 tgcttgacga ccttttaata ctaatggctg aattaattta tcaactttgt ttctcgtttc
    31561 catatccaac gaaccataga aagctgccca ttcatcaaaa aagataaaat gcggcggtat
    31621 cccataataa gcataatttt tccctggttc atacttaggc tctttcatcg ttttcatata
    31681 ggtatatcgc tgattcattt cttgtaacgc atttcgtaag caaataatca tttcttcatt
    31741 cgttttgtaa aacacatgtc ctttgaatac atcaatatca cctaatgccg ctaaatctgc
    31801 cgctttccca tcgcaaatat ctactgtccc tacatctaaa caagctttga tcaatgaata
    31861 caataaatag gtttttccgc caccaatcgc accagaaatt aatagatttg gcgattgatc
    31921 atatacccaa tccaatgttt tcattaaatg aatacgtcca ttttctgcta tacattgatc
    31981 aataggaatc cgaccagaat cggaaagaaa tacataattg attcgggctt tttctcgttc
    32041 ttcacgaatt aaatcacgca aaaatacttc ggataataag ctacccattt ttaaaaatcg
    32101 ttcgtggaaa cggccaccat ctaaaggaaa agaaaccact aattcttgtt tcttttgata
    32161 aaaataaact tttggtaatc tcattttttc cttcgtttgt ttctcaccaa cttgcttctt
    32221 tgtataaaca aaccgattgt caattaacat tcgagcaagt aactgacgcc aataaacaga
    32281 agcaaaatat ccttccttta gtcgcgacca acgcatcaaa cctaaaatta atccactaac
    32341 cagcaacaaa tacatcacaa tactgattcc aggaataaga tacatttgcc aattcgaata
    32401 cgtatttaaa cgaggataaa atactttcca actaaaccaa cttaatagaa tcaaaaatgg
    32461 taacagaaag aaaccataat aacaaaacca taaataacga ttgaaataac gaattcgtcg
    32521 accacgatac ttaaaaaaat gaggttccaa ttgtcacatt cctcctttct gtttttactt
    32581 ctcctgtgct aaaaattttt gacaaaaagc aactaatact ggaatatcac atttaataaa
    32641 aaaccataat cgaaaaagat ttaatcgatt tttatatggg tgtgcacaca tattacatat
    32701 tcgtactaca cgaatccaaa ccacttcctg atggtcttgt ttccactgat acggtaatga
    32761 aaaacgaaca agatcacgta aaaacataca ctgcccaata accgcacaaa tatactcatt
    32821 tgtttgtata aaaatcgttg gattgtctcc aaaatactca atcaattgtt ggatattttg
    32881 acaacaaacc ataatctctc gaagtgtttg attggttttg tccagattca aaatctcccc
    32941 ttccttctac cttaaataga aagagtagga agacttcctc ctactcttta aaaaacttat
    33001 tttttctctt tttccttaga atcagcactt ggttgttttt gatctaaacc tttttcttgt
    33061 gattgtttcg gattagaatt atttgttgga gcttctactt tctctaatcc tttcgcataa
    33121 atcactaaat ctgaatattc acgattgcca tcttgtgctg gaatatatac cacatgatat
    33181 tctggatcaa ttaatttcac aggatcacgt cgtttaaaac cagtcaaatc tacatctgct
    33241 ggaaatttaa ctcgtcgtgc aatctttggt tcatctggat ttccaaaaac taattgtgct
    33301 cgctgtgatc gaatttctcc cgtataatcg cctgtttcat tatcaaatac acgatcggac
    33361 tcttctaatg cgccaaagcg tacggctcct atacaagttt catctaatgc ttctgtaata
    33421 tcataattat ttggaaattt taatcccatc ttacaaaacc tcctcaattt ttacaccagt
    33481 taaaaagaca ccgacatctt gatagccagt tgttcgacgc tctgtttgtc ccatatcatt
    33541 tgtttcacga ctattcactc tttctttacg aaaaacaaaa taagcttctt caaatgaaat
    33601 acgtacctct ttttctggta atcgtaatct tttatcctct tcagctgtga gataaacaaa
    33661 aaaagcaggc tgttcttctc gcgaacattc tgcttgtaat tgaacatatc gcccagacac
    33721 accattaatt tcctttggtg gattaggttc ccgtgaaccc aataaaaata cctgacctaa
    33781 aaactctttc ttcatacgca atgctttttc tgccatatca tttttcctcc tcttaaaatt
    33841 cacaatcaat tctaaacgac tctatattac acaatggaaa gtaatgttta acaacaaatg
    33901 gctcatcact aggagacata tctactatta acatcccgtc tcgttcatgc acagaaatat
    33961 cagtacactc ctcatacgta cattcttttc cactcaaaaa acaaatattt atatttttct
    34021 tttccatatt cttttttcct ccattatttt cttttctgta aaaaccaagt aataattcct
    34081 tttccatctt ccaacacaaa ctgatgatat atttccctag caagaatcca cacacatgct
    34141 aaaataaaga aaataataat actactaaag atgcactcat ccttgctgtc aaacggaaaa
    34201 agatatatca taattgcctc ccccataatt aagaaaccaa ttgatacaaa caaaaactct
    34261 accatcatac aaaagaatat ttcttccaac tcttttacat cctttctaac tttttataaa
    34321 aaaggagatt atttttataa acacttttat tcaacaacta ttttcaaatg aattacacat
    34381 tatcatctca agtatagttt caatcgtcac tattgtttgg tccatttcta gcttttattt
    34441 aaaacatcgc tatacaagaa aaaagaaaag cactgaaacc ctaacaaaag aaatgtttat
    34501 tccatttaaa tcttcaattg aaaattatct atttcaaaaa attacaagaa aaaatattct
    34561 agaacgaaaa caaacaattc aacatctaat ccaaacagtt gaaaataaaa atatagactt
    34621 ttatttgcct tatgaactta tttattttgc caaagaaata caaaaaataa ttaacacaaa
    34681 taaacattcg aaaaaaatcc tcttcaaagt acaaagaatg tattttgaat tctcatactg
    34741 ctacctagca gaactaaata aagtaagaaa gcaattagga gtagctaaac gtgatttctt
    34801 ctatagaaaa aaagaaactg tattatcaca taattatatt ctatctcttt tggatcctta
    34861 tattcttatt tattacactc agtatttctt ggtgtatcct catgatcatt tactactcaa
    34921 cacaataatt tatttgcctt tgataaaaaa ttctttctaa aaactaaaca agcggctagt
    34981 tgcctaaccg cttgttcgtc ctctttagtc tctgacttta tacaaatcat aaccaatcaa
    35041 tcctgccgca aggacaaaga tcataccaat catgatccat aatacattgt cttcagagcc
    35101 tgtttttggc aacataggta ccattgcagc ttgtttcact tcttttacag gttgtggttc
    35161 gtttggtttt actggttgcg gtggttttgg tgcctccaca aattgaaccg tttgatcttt
    35221 gtttgttaga tcgacgttat gttttgcgac ttcttcaaac gtttcttttt catcttctgt
    35281 tttggcttgg agccattccg caaaactcaa tccttttcct gcaaggttgc gaccgtcaac
    35341 gatgaattca aacaagaatt ccatcatttc tttatcagcc ataaagactt tttccgcatg
    35401 tgttccttca aatggggtgc cgtctggtgt taagtagcct tgaacaatca gtttgtaaat
    35461 ttgaccgatt gctaacccat taacttgaac ccattcgaca accttttgat ttggtaaagc
    35521 taagatctct tttccaccat ttgcattggt agcttttgtc gtaatggttg gactgacgat
    35581 tttgatggtt tggtctttgt cggtaatatc tgcgtgagaa gccaataata cgccttctct
    35641 aaacagatct tcaaaaacta cgacttcttt gcttcgcaac gcatgtgcgt ctaggataaa
    35701 gtctagattg actgtaccat cagttgtttc tgccacaaac gttaaggtac ttgagacttc
    35761 ttttccatct actagcactg gtttttccgt tactttgtcc attagaacac cgttgactgt
    35821 gtatgttttt ccaacgatca aatcgtggta tttcacagcg tctgtaatgg tgactttcgt
    35881 taatggatta acctctttca agccagtggg tttatgtgta gccgttgtac gtacacttgg
    35941 gttcgttact cgtaccgttt gacctttgtc gttgatgtct ttatggtttg ctagttcttc
    36001 gtcatttcta aaaagacttt caaacacgac catttcacga ccatttaaac ctgtcaaatc
    36061 aaaggtaaat ggtacgatca ccgtttcatt ttctgctttg gcaataaagc caacagcact
    36121 ttctaccggt tgaccatgaa caagtaaggc ttcaccagtt gctttatcca ttaagattcc
    36181 tttgaccaag taagcttttc ctggaaggat ctcattaatt tcaacggtat catttaaggt
    36241 gaacagcccc gttgggtctg cgatcgtcaa cccgtcttca aatacagctg tggtatgaac
    36301 cgttggattg tttacccgaa cactttgatt gtcatcattg atatccacat gtaccgcaac
    36361 ttcaatgcca tcacggaaaa gtttttcaaa gacaacgatt gtttgacctt tcagaacact
    36421 agcattaacc acaaattcaa gatcttttgt gccattggct ttgtctgcaa taaaggtcaa
    36481 gatacttgtg atttctttgc cttcttgtaa aattggcgca ttcgttgcct tgttcatcaa
    36541 tacacctgaa atagtgtatt ctttgccgac aaacaggttg gtataagaaa cactatcttt
    36601 aatgacgata gtttctaatg gatcaaccac tttttcacca tcggctttat ttgacgcttg
    36661 tgtttctaat tttggtggaa tcaaatagtt acgtacagaa accaataacg tttccccatc
    36721 atctggcgca ttttgtctga atgtaatagt gaatgcttgt ttgcctgtat ttggttggaa
    36781 actttctaat ggtacatttt cggtaaagta atagttattg gcaaccagac cttttactaa
    36841 aatctcacca ttttgatcgg tggtatattt accaattgct tgaccttttc cgttatatag
    36901 cgtaaacgtc acaccggcaa gtggtgtttt cttcttgttc cattcttcat acttgaacaa
    36961 ttttacactt ttccgtgctt gacggttcgc aaaatggatc actttgatcg gttctgtatc
    37021 attggtaatc gagaacacta atgggttttg attcaaaacg taatttacgg gtgctttgat
    37081 ttcttccaaa cgatagcgct cttttccata agtaagtgta ccgttagtta ggaaaaagcc
    37141 tttttcattg gttttaaaga tatccgttgt ttctgtatca tttggaatct ccattgtgac
    37201 ataacgattg gctaaagtat caaaaatctt gaattctgta ttggcaagtg gaatcacttt
    37261 tcctgtttct tgatccactt tcaccacttt aagacgttgt tcaattactt tatcttctaa
    37321 aacataatgg aaagtttgcc cattttcaga aattgtgact tcaagtggtt caaatagttt
    37381 atacccttct tttcctttgg tttcagaaag aatgtaggta tcaaagacaa cagtattttt
    37441 accaaaagta gcgtaaccat cattgtctgt cacaacggtt ttcaccactt tgccagttgt
    37501 ttttgacgtc aaagagattt caatgccagc taattttggt ttgttatctg gattggttga
    37561 atcgactaaa tctttattag caattttcac taaatcaatg gttcctttga tcacttgatc
    37621 ttttgcggtc gcattgacag tttttcctgg ttcaacaaca atttcaattg tttcatcaga
    37681 aagtgcatag cctttcggcg ctttgatttc ttttaaaaca tatttgccag gatcaagatc
    37741 tttaatttct gcctgtactt tgccgttcat atttttcaag gtaatttctt tgatcttttt
    37801 cccatctgct tgatacaagc catacaccgc attttctaaa gaagctgcgc cttgtgcttt
    37861 cacaccggtt tctgcatctt ctttaaagac acgaatgttt ccctgtttca aaatagtcaa
    37921 attgacaaca gagtcaccag gttcaaaagg attcaatata ccaattgttt gggcacctgg
    37981 acgacgatac gctaatggcg tacccaatac ttcaatctga ttgaaatgaa tttttgcctt
    38041 ttctggtgct tcttttgtga ctgtgatcca tgcttcattc ccatgaatag atacattgat
    38101 cccttttggc ttttgaatgc cttttacacg ttgtagaaag tcattgccac tttttacttt
    38161 taacgtttcg ccaattttta tcgtatgtgt ttgattatga aaagtagctc ttacatcaaa
    38221 attattaagc agacgttgaa tttctgcttt tcgacgatca taatcaacac ggatattttt
    38281 gattgggatt tttgtgccca aagcttccca aatcataaat tgtgccgcaa tgtattgttc
    38341 atcggatttg tctttatttg tgacatatcc taaataagag atcaagcttg tttttttccg
    38401 tacatttgaa tttttcaaat agttttccaa agcagtagct gtataaactt gaccttctgc
    38461 ggctgagaca ccaggctcaa tacaaaacgc cacttgtcca ttaatctcaa aacgaaccat
    38521 ataatttaaa gtgaagtttg gattgttcgg actagaagta acaccaatat tttcgcccca
    38581 atcaattttc cgaccaccta aaacagctgt attggttgca cggcgaacac gaaccgaacg
    38641 attggttggt actgttacta acagtgtagg tgtacctgtt tttggaatgt cctgctcttt
    38701 tttcttttga tcttcaatac tagccgtaga ttctttgttt agtttcgttg gttgattttg
    38761 actagctgcg gttgaagaga ttgttttttc aacgttttcc tcattttttc cttctttgtc
    38821 agcagtagcc gctggttgtt ccactgtttc ttctattcgt tgcttttctt ccgcttgttt
    38881 ctcagaagac tctggcttca cagaaggcgt tgcttcggca gctttttctc cttctggagt
    38941 ggacgtttct gaagcagggg ttgtaattga ggcagtttct tcaactggtt gtgaagtaac
    39001 aggactattc cctgggtgtt gtgtttcccc aatcgtctga tcgagagtcg ttttggaatt
    39061 aaccgttggc gcttctgtca caggggcctg ttcaacagtg gcttcaccag ctgggacccc
    39121 ggcttctgct ggttggactt ctgtcacttc agaagcgact tgggcaatgg cttccaccgt
    39181 aggttgtgta gccgtcgttt cttctgcatg tgcttgagca gttcccacca agaaacagaa
    39241 ttgagataag gccaccaatg cgagtgcttt caaccaattc attttcgttt ttgcatacac
    39301 aagaattcct ccttttcctg agaattttat ataataaaac aaaacaccgt taccaaaagt
    39361 gataacgacg tgaactgtct cttctatttt tatctggcgc tcttatctga acacatttca
    39421 tcaatgattt taccctccca ctataattac agaacaaaaa aagaacctgc ttcgtggaaa
    39481 tcaactgtcc atttcccaat tggaatacca gctttattgg caacttcacc atcaccaaat
    39541 atcatgccat accaattact accttcatcc ttaatttctt tccaaccata tgacatagct
    39601 tcatctaacg aatcaaaaat catgccactg ttgccaattg ttgcaggatc tggcttaaga
    39661 tcttctggaa tagaaatatc aatcacacca taatgatccg attccccagt attattgtca
    39721 ttactagcta ctggtttggt tgttctactg acttattttg atacaacaag tacgcttatt
    39781 cttcttttac ttctccgttt tcaatttccc attcaaacca ctccgcaaag tcttgcttaa
    39841 aaccatctac tcgataccta ttttgtaaat aggcatcttc tgacgtcgta ggatctaaat
    39901 aggtaggttt attctctgga cgagcataga cacctaccca tgtttcgtaa tctccttcaa
    39961 aataactatc taccaaatag cctttttttc taagaatctc ccgaacttga tttttaatta
    40021 ttgatacttg ttcaaaacca tcactcattc tatcacctca aataactcta gattctcata
    40081 aatattcccg acaatttcaa tcctatcaat gatctctaac aaatcgctac ttatgcttcc
    40141 ccattcgtat aagaatttcc cttccgcaaa ataaattttc cataaacttc ttcatgatcg
    40201 tcccaaccaa tatcactctc gacaaattcc acgttgttct tatcttttag ctcagtaaat
    40261 gccataagtt caacatgttt tccaacctcg aaacaatcac accacccatc aaccgttaca
    40321 cgtgtgcctt caatacctaa gaaactttgt cctcgtgtat taaacactaa aactttccgc
    40381 attttttgct cattaattaa tcacgcgcga aatctaggaa tcacaacttg ttcgcttctt
    40441 ctttataaaa taaaaaaact ccaataatcg gaaattacta tcttttcctc attccttttt
    40501 taaatcttca tacttcttct tatctttgtc ctaacctaat aacacaaatt ttgaagaaaa
    40561 actcagtaaa acctgattct ttttttatct tcacaacaac caactttttc ttcagatcct
    40621 tttactagaa cttgtataca aaaattgttg aatgctgata caatttgcgg ttacttgaaa
    40681 aaacgataga tgtaaaaaga taaatagaat cattttttta ttctatatca gtctatgatt
    40741 cattgcttat caatcaccca acccattcga tgccgttttt ttaagagaaa tttaaaaaag
    40801 accgtacgaa tctcgcattg tccgattact tttctagaaa agatccccaa ttctgtataa
    40861 ataaccctat cttcttgttg gtttcatttt ctcatataag aattcagtca atctacccac
    40921 tatgaccatt actacgtcca acacaaccga catattaacc acgtaagtat tccctcacag
    40981 atctccgaat ttccatggta tggattccca tcctgacgct gcagaggaca acatatttca
    41041 tgtggctctg tacccttttg atttggaaag actacaaatc aatactcatc tataacttat
    41101 caagcgaact ttcgctaggc ttctagtgat tactaaattg ttggataaca tacctagccc
    41161 attttatcta gctggtattc ataacagtta tttcttgtat tacttcttcc gcttgtgttg
    41221 ccattgagct tgtcaaaaag ttacttagaa aaaagctgcc agtaaagcaa ctttcaacaa
    41281 accctttttg tttcttatca tatcaagaaa cctcctttta taaatttttc tataataaaa
    41341 aaacgtcgat aacttaataa gtatcgacgt aaactatttt tatatcccgt aaatcaaaaa
    41401 ttctaaagaa agatttccta tataagcata ttggataatt cgattagtat tgggtatatc
    41461 tctaccattc tcatacctat ctatggacca ttttgaaatt gaaggagaaa aaagctttcc
    41521 aaactcctcc ctttctagat aataatcttt cctaattttc ctcatcctta atcccatact
    41581 aaaagaattt atcttgttaa aatcgctaac tctttcatct tttggaagta tattcttata
    41641 atcttctccg tacataaggt aaggaacagt gaccttacca agataggcaa tattcattag
    41701 tttttcaata tctggaatat taataccttt ttcccatcta cttataacat ttttgcctgt
    41761 atagatggta aagtttttac caaatttttc aagcgtctct tcccggttct ttcggacttc
    41821 tcgtattctt tttcccataa caattttatc gtcttttgat aaactaaata tctcactatc
    41881 acgattactc tccttcgaaa tttctttttc attacttaat agaaaaatat cgattttttt
    41941 ttgcatttct tttgacggat ttctctttct atttaacaca taactgagta atacttcact
    42001 aattcctata ttatcagcta actctttgtt aagtaactta ttcatatctc tgtatgcaat
    42061 tagttgaccg atgagaatct caatcttatc ttcctcatct tttattagag tttctatatt
    42121 caaaatcttg cttagagtat tatctacgtc tttagggatt tttcttgacc ctttttcata
    42181 tttaactaaa gtactttgtg aaatacctgc taaatcagct aacttttttt gagatagttt
    42241 tttctctttt cttagagatt taagaaccct tccacttgaa ttatcaacca tgaaaaaagc
    42301 acctcctatg agatgctttc atcatatact gacagctaaa aatcttcaag aaaaaatatt
    42361 ccaattttgt taattttagt atcgccgtga taaccaaatg acagtttaaa atcttttata
    42421 cagataatcc tcagtgttgc atctactcaa aaaggttgat ttaataggtt tttcgattga
    42481 ttataagtta tttcacacta tcttgctc
//
LOCUS       DAYLZO010000010        36586 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.010, whole genome shotgun sequence.
ACCESSION   DAYLZO010000010 DAYLZO010000000
VERSION     DAYLZO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.010"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..185)
                     /locus_tag="ACGVJG_000497"
     CDS             complement(<1..185)
                     /locus_tag="ACGVJG_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556481.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPR"
     gene            complement(151..1482)
                     /locus_tag="ACGVJG_000498"
     CDS             complement(151..1482)
                     /locus_tag="ACGVJG_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFJ6556482.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1738..2304
                     /locus_tag="ACGVJG_000499"
     CDS             1738..2304
                     /locus_tag="ACGVJG_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6556483.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            2449..2536
                     /locus_tag="ACGVJG_000500"
     tRNA            2449..2536
                     /locus_tag="ACGVJG_000500"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2481..2483,aa:Ser,seq:cga)
     gene            complement(2588..3793)
                     /locus_tag="ACGVJG_000501"
     CDS             complement(2588..3793)
                     /locus_tag="ACGVJG_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFJ6556484.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(3931..4068)
                     /locus_tag="ACGVJG_000502"
     CDS             complement(3931..4068)
                     /locus_tag="ACGVJG_000502"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFJ6556485.1"
                     /translation="MTLQPGEGTKQIAERAGITVDQLFALNGMDPNNYMLYPGQELRV
                     K"
     gene            complement(4370..4819)
                     /locus_tag="ACGVJG_000503"
     CDS             complement(4370..4819)
                     /locus_tag="ACGVJG_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HFJ6556486.1"
                     /translation="MKKVITSLLVFASLLLFTACGKKVTTEDLKANDWLIEATKDDEP
                     NMIVSFSDHVVSFSVDTSSMKSTSSNDWEAMGEEFAKSLVDQMNYKFEYTLEGDVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKPNKSDDNEKLVLKPYKKEKN"
     gene            complement(4906..5106)
                     /locus_tag="ACGVJG_000504"
     CDS             complement(4906..5106)
                     /locus_tag="ACGVJG_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556487.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(5135..5557)
                     /locus_tag="ACGVJG_000505"
     CDS             complement(5135..5557)
                     /locus_tag="ACGVJG_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFJ6556488.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            complement(5574..5996)
                     /locus_tag="ACGVJG_000506"
     CDS             complement(5574..5996)
                     /locus_tag="ACGVJG_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6556489.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            6287..6478
                     /locus_tag="ACGVJG_000507"
     CDS             6287..6478
                     /locus_tag="ACGVJG_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556490.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            6489..6755
                     /locus_tag="ACGVJG_000508"
     CDS             6489..6755
                     /locus_tag="ACGVJG_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556491.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            6767..6937
                     /locus_tag="ACGVJG_000509"
     CDS             6767..6937
                     /locus_tag="ACGVJG_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556492.1"
                     /translation="MGKFNRTLVFSAPLIIYALGLWGSRQALIGTIVYMLWIFIGLDE
                     AEYRAKKPVGRD"
     gene            complement(6996..7475)
                     /locus_tag="ACGVJG_000510"
     CDS             complement(6996..7475)
                     /locus_tag="ACGVJG_000510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556493.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            7520..8227
                     /locus_tag="ACGVJG_000511"
     CDS             7520..8227
                     /locus_tag="ACGVJG_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFJ6556494.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            8220..8348
                     /locus_tag="ACGVJG_000512"
     CDS             8220..8348
                     /locus_tag="ACGVJG_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556495.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            8406..8747
                     /locus_tag="ACGVJG_000513"
     CDS             8406..8747
                     /locus_tag="ACGVJG_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556496.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHFYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            8740..9486
                     /locus_tag="ACGVJG_000514"
     CDS             8740..9486
                     /locus_tag="ACGVJG_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFJ6556497.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            9492..10178
                     /locus_tag="ACGVJG_000515"
     CDS             9492..10178
                     /locus_tag="ACGVJG_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFJ6556498.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            10185..11018
                     /locus_tag="ACGVJG_000516"
     CDS             10185..11018
                     /locus_tag="ACGVJG_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFJ6556499.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            11035..11886
                     /locus_tag="ACGVJG_000517"
     CDS             11035..11886
                     /locus_tag="ACGVJG_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6556500.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            11883..12242
                     /locus_tag="ACGVJG_000518"
     CDS             11883..12242
                     /locus_tag="ACGVJG_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFJ6556501.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            12257..12418
                     /locus_tag="ACGVJG_000519"
     CDS             12257..12418
                     /locus_tag="ACGVJG_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFJ6556502.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            12421..12720
                     /locus_tag="ACGVJG_000520"
     CDS             12421..12720
                     /locus_tag="ACGVJG_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556503.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            12720..13034
                     /locus_tag="ACGVJG_000521"
     CDS             12720..13034
                     /locus_tag="ACGVJG_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFJ6556504.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            13211..13522
                     /locus_tag="ACGVJG_000522"
     CDS             13211..13522
                     /locus_tag="ACGVJG_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFJ6556505.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            13519..13737
                     /locus_tag="ACGVJG_000523"
     CDS             13519..13737
                     /locus_tag="ACGVJG_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFJ6556506.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            13750..14031
                     /locus_tag="ACGVJG_000524"
     CDS             13750..14031
                     /locus_tag="ACGVJG_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556507.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            14028..14354
                     /locus_tag="ACGVJG_000525"
     CDS             14028..14354
                     /locus_tag="ACGVJG_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556508.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            14351..14548
                     /locus_tag="ACGVJG_000526"
     CDS             14351..14548
                     /locus_tag="ACGVJG_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556509.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            14545..14754
                     /locus_tag="ACGVJG_000527"
     CDS             14545..14754
                     /locus_tag="ACGVJG_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556510.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            14751..15038
                     /locus_tag="ACGVJG_000528"
     CDS             14751..15038
                     /locus_tag="ACGVJG_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556511.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            complement(15071..15322)
                     /locus_tag="ACGVJG_000529"
     CDS             complement(15071..15322)
                     /locus_tag="ACGVJG_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556512.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            15630..16097
                     /locus_tag="ACGVJG_000530"
     CDS             15630..16097
                     /locus_tag="ACGVJG_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFJ6556513.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            16396..16737
                     /locus_tag="ACGVJG_000531"
     CDS             16396..16737
                     /locus_tag="ACGVJG_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556514.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(16730..16921)
                     /locus_tag="ACGVJG_000532"
     CDS             complement(16730..16921)
                     /locus_tag="ACGVJG_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFJ6556515.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDVTK"
     gene            17271..17483
                     /locus_tag="ACGVJG_000533"
     CDS             17271..17483
                     /locus_tag="ACGVJG_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556516.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            17490..17714
                     /locus_tag="ACGVJG_000534"
     CDS             17490..17714
                     /locus_tag="ACGVJG_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556517.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            17741..18274
                     /locus_tag="ACGVJG_000535"
     CDS             17741..18274
                     /locus_tag="ACGVJG_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFJ6556518.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            18261..19505
                     /locus_tag="ACGVJG_000536"
     CDS             18261..19505
                     /locus_tag="ACGVJG_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFJ6556519.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            19518..21017
                     /locus_tag="ACGVJG_000537"
     CDS             19518..21017
                     /locus_tag="ACGVJG_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFJ6556520.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            21022..21948
                     /locus_tag="ACGVJG_000538"
     CDS             21022..21948
                     /locus_tag="ACGVJG_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFJ6556521.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            22075..22677
                     /locus_tag="ACGVJG_000539"
     CDS             22075..22677
                     /locus_tag="ACGVJG_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFJ6556522.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            22690..23613
                     /locus_tag="ACGVJG_000540"
     CDS             22690..23613
                     /locus_tag="ACGVJG_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFJ6556523.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            23631..23894
                     /locus_tag="ACGVJG_000541"
     CDS             23631..23894
                     /locus_tag="ACGVJG_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556524.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            23906..24235
                     /locus_tag="ACGVJG_000542"
     CDS             23906..24235
                     /locus_tag="ACGVJG_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFJ6556525.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            24232..24558
                     /locus_tag="ACGVJG_000543"
     CDS             24232..24558
                     /locus_tag="ACGVJG_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556526.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            24542..24880
                     /locus_tag="ACGVJG_000544"
     CDS             24542..24880
                     /locus_tag="ACGVJG_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFJ6556527.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            24880..25272
                     /locus_tag="ACGVJG_000545"
     CDS             24880..25272
                     /locus_tag="ACGVJG_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFJ6556528.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            25283..25795
                     /locus_tag="ACGVJG_000546"
     CDS             25283..25795
                     /locus_tag="ACGVJG_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HFJ6556529.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            25841..26209
                     /locus_tag="ACGVJG_000547"
     CDS             25841..26209
                     /locus_tag="ACGVJG_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFJ6556530.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            26263..26550
                     /locus_tag="ACGVJG_000548"
     CDS             26263..26550
                     /locus_tag="ACGVJG_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556531.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            26567..29686
                     /locus_tag="ACGVJG_000549"
     CDS             26567..29686
                     /locus_tag="ACGVJG_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFJ6556532.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            29698..30486
                     /locus_tag="ACGVJG_000550"
     CDS             29698..30486
                     /locus_tag="ACGVJG_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HFJ6556533.1"
                     /translation="MDQRENKMYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGY
                     QTLTVEGREMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELL
                     NYLYRNEDVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVS
                     DGYIGNPIQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQV
                     TVNGEDCTSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            30483..33314
                     /locus_tag="ACGVJG_000551"
     CDS             30483..33314
                     /locus_tag="ACGVJG_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFJ6556534.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGATGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            33289..35046
                     /locus_tag="ACGVJG_000552"
     CDS             33289..35046
                     /locus_tag="ACGVJG_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFJ6556535.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            35063..35845
                     /locus_tag="ACGVJG_000553"
     CDS             35063..35845
                     /locus_tag="ACGVJG_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556536.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            35845..>36586
                     /locus_tag="ACGVJG_000554"
     CDS             35845..>36586
                     /locus_tag="ACGVJG_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFJ6556537.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLK"
BASE COUNT        12819 a         5871 c         7590 g        10306 t
ORIGIN      
        1 cgaggattgt tcagcaaatc agcaagtaca tctaaatctt ctggctgagt ttctgccgta
       61 ccctgtatct ctatgaaaag cccttcctca gcaaattcta ctccaatacg tatttcagtt
      121 atttcatggg aatataaata agtaagaagc tcatcgatga ttctttttga taattctttt
      181 tccaagtgtc tttttttcct ttctgacagc ggtaaagtta tctagtatcg gtacaaggat
      241 acctgcaacg aaaccagttg aaaaaccact gttatataga tttagtcctc cgtgcaaagt
      301 gctgacgtta tggacaagag caatatgtag tacaccagct attacaccgt aaaaactgcc
      361 gtaaaaaccg cttatgggcg cgagacctgt cccaaacaca gctgcaacaa gcatgccagt
      421 actggtgatc tcatgaaaag ttccaaacaa agaagcaata aatattccga ctagtatcgg
      481 aaaactgttt tttaaatgac aaccaaaagc actgaaaccc accgcagata aaatagcacc
      541 gatgacaggt ccatttaact gaccgccaac cagcaaaaca taagaagaca gcatcagacc
      601 aactaagcct atgttgatca aagtagcacc aataccagaa ttagcaatga aatcagttgt
      661 caattttcca gatgaatcga aaatttctcg aatccccaaa agccgaaagg aattataata
      721 gaaaccaatt ccgactgtca gcgaaaaaaa gccaaaaata atccaaatga gaggaaagtg
      781 atattcattg aaaaccaatg tattttcgac gattgaatag ctgaataacc tcaaaacagc
      841 agtaaaagtc atcgcaatca acccagaggt gaatccaata ttataaagtg tgaatccacg
      901 atggaaaact aaagtttgag cagctaaggg cggaagaagt agaccaattg ctatgccagc
      961 taaattcccc aataaaatac ctatcagtaa aggaaaacgc ataccaaaag tgatataact
     1021 aacgaccggt gataatgcgc taccaaacag tccgatcatg atgtactgag aaaaaggttt
     1081 attgacaaat acagcgtata gatacacacc taaaatgatt gagatagaat tataaaggtt
     1141 tttgccaaaa aaagagaaac cggtaactgt tagaatcgaa gcaaataagg gaccgtttaa
     1201 aatcaccttg tgccgataag ctaaaagcac agaaagtaag gtcaaaatcc ctacatttat
     1261 gaatgcgctt ccgaaaccgc ctaaagcaaa ataatctgtc aaaagattgc ttggcgaggt
     1321 aagaatagtt agcaatccac tccaattatc agtaggagta ttagaaagca agccaataaa
     1381 aagaaaaaaa acacctgtaa aaccaagata aaaatacttt cttttctgtg aaacgattcg
     1441 tttttgagga tgaatataaa taacgtcagt ttcgttttcc attacacacc ttctttttca
     1501 ttttctttaa ctataacttg aaatgaaaga tttggacaac tatcatttag gtcaaaacta
     1561 aaaatgtaag ggaacccatt gaatttaacc aaattttttt aattttgcaa aaaaaagtta
     1621 aagaagactg aaaaaaaatt taaaatataa cgttttgagt aaaatgtttg caaaaaatgc
     1681 taaaaatagg gtacaataaa acaacatata ctgcagtaaa gaagtggggg agttttaatg
     1741 agaagaaaag tctataccaa ggatcaaatt ctaaaagctg cttatgatgt tgttgcaaag
     1801 gaagggttta aaggatttac tgcgagaaat attgctaaaa agatggggat ttcgacacag
     1861 ccgatttatc tggaattcaa aaacatggaa gatttaaaaa atacgttgct ggattctatc
     1921 tttgaacaac taaaagaaga tgttttccct attgaacgta ctggtgataa aatcattgat
     1981 ctcggtttga actatatcta ctttgcaaaa aagcatcata gtctttacat ggcattgtac
     2041 attgatgaac atggggacgg aaagaaaatg catgattttt catacaatta tttccgtgaa
     2101 atagttaaag aagatgaaag atacaatcaa ttatcggatg aatatgtcga tgctctgcat
     2161 actggtacat gggtagcagt gactggctta gcgtcattga tgtcatcaaa catcatgcat
     2221 ccaacagaag aacaaatcat tgcactgatc aaacggacaa ttgactcgat tttagcatta
     2281 gataatcctg aaataatcag atagtgttct atcagtatga atttgatcgg taagtccgtt
     2341 ggctgcaaac tgtcaatgga cttttttgct aaaaaaagcg atttctgtta tttatctatt
     2401 agaaataggc ttgacatttc gtgaaaaaag tttaaaaata gtacttacgg aaggttggca
     2461 gagtggtaat gcaccggact cgaaatccgg cgaaccggct aataccggcg cgcaggttca
     2521 aatcctgtac cttccttata tatcaacgat tagacacgat ttcgatcgtg tcttttttgt
     2581 tgtatattta gcctaaaatg ttttttgact ccccttttga ctccccctga gtactgttct
     2641 tcataaatgc tacaaaatta ttgatgactt ttttgttttg agtttcagtt acatgagtgt
     2701 aaatatttga agtagtttga atgtctgcat gccctaaacg ttcctgaaca tcttttaaag
     2761 aagctcctga ttcaaaaagg agagaggcat gagtatgtcg aaaaccatga gtagtgattc
     2821 ttttagtcaa ttctggatga tgcttatata tgacattaag ccacgaacgt gaaactgtag
     2881 ggttatagag aagaccatca tggtgaaaaa ttaagttttc cctttggatt gtaacgatcg
     2941 ttttgttttt tgtataatac tttctcaata aactgatcaa atcattgtcc agataaattt
     3001 ttctttttcc agattttgat ttaggagtat ttacgatcaa tcttccttga taacctcttg
     3061 ttacagtttt attgatattg agtgttttgc tcttaaagtc aatatccgac caggtcagag
     3121 caaaagcttc tccttttctt atacctgtaa aagccaatag ggaaaagaat aaatatcttt
     3181 catcatcatc gtttcttatt gcttctaaaa atttgattaa ttcttcttta gtataaaatt
     3241 cgatattttt atcttcgata tctagagctt ctccacgtgg aatagaaact aatttcatag
     3301 gattgctagt tataatttgt aaagaagcag cgtaatcaaa aacattagat gtgtaatttt
     3361 ttattttctt aaaaatttta ggatggctat cggaccaagt gtttactgct tcttgacaga
     3421 aaaaaacgtc tattttgtca atgaacttat ctccaaacac ttttaagatg tgtgtgtcaa
     3481 aaatttgttt ggtggaggac caagtgcttt ctttaactgt ctttttataa ttttcaaacc
     3541 ataagccgta aacatattcg aatttcttgc tcttttgagc tgtagaagct tgcagtcctt
     3601 tttcctgtat atctgcctcc aatcttttta aagctctctc agcggctaat ggcgtactaa
     3661 aacctcgtct cgttgttttt cttttttttc ctgttagagg atcaactcct aagtacagac
     3721 taaactgata tttttcttcg cctttttttg ttaagtgctt tttaattcgt ttatcaattt
     3781 ctttttttgc catctttatc tttcctttcg tacgtttgtt cggttgtaca gcggatttcg
     3841 agatggtaaa atagggtaca acaaatagac ctactttacc gtagctcttt gcacatttgc
     3901 gttcttggtc gggcggcaaa tgtgcttttt ttatttcact cttagttctt gtcctggata
     3961 aagcatgtag ttgtttggat ccataccgtt taatgcaaac agttgatcta ccgtaattcc
     4021 agctcgttca gctatttgtt tggtcccttc accaggttgc aaagtcaagg tttcggtttc
     4081 agatgaagaa ggtgccgttg gttgagcttg attagctggt acataatctt gtggtactga
     4141 ttgctccgtt gaactgggag tgggtgaaac ttgttcaaca cttgaactag gagtagcttc
     4201 tgcatagttt tctacacttt cagcagagta agtactacta gtttcaggag gcgtattctg
     4261 cgcaatagca gcttgttcta cagtactaga agatgcaata gtactttctg ttgtagtcga
     4321 agatgattgt tcgttgctac ttgttgagct agtagttgtt ctagattctt tagttttttt
     4381 cctttttgta gggtttcaaa actaatttct cattatcatc agatttgtta ggcttttctg
     4441 gagttaaaat aatattttta tcttctttgc tgacagtata tttggcatca ttatctttgt
     4501 ctttatcatc tttccaagtc ataacatcgc cttcaagtgt atactcaaat ttgtaattca
     4561 tttggtcgac taagctttta gcaaattctt ctcccatagc ctcccaatcg ttacttgaag
     4621 ttgatttcat agaacttgtg tcaacactaa aagatacgac gtgatcagaa aatgatacga
     4681 tcatattagg ttcgtcatct ttggttgctt caatcaacca atcattagct tttaaatctt
     4741 ctgtagttac ttttttacca caagcagtga aaagtaacag agaagcaaaa actaataaac
     4801 ttgttattac ttttttcatt ttttattcct catttctatg atatgatttt tattggggaa
     4861 tcttagaaat aaggtttcga gtccgtgttg ctgcacgggc tttttttatt taaataaatc
     4921 ccaaaaactg aatgtagttt tcttgtatac tttattgtaa gcagcctttt ttgggtcttt
     4981 aatccagccg ctaccttttt tcccataacc aggaataact gcttttttta cttttctttt
     5041 tgcttttcca gtggttctag cgcttatgga tttttttata cttggttttc tcattcctat
     5101 tttcatctat atcctcctta cgttgtatta taattcacaa gaaacgatcg aattcatctg
     5161 gtaatccaaa gcagacgagc ttttcatatt ttgtcatatg atcaaagccg tctaaagttg
     5221 gatcaatagt cagatgagtt gcgaaataat ttgcttcttt ttcaattttt aattcagata
     5281 ctagcgtttt tttagacaac ataggtgtgt tttcattcgg atgcaaaaca cagtggccta
     5341 attcatgact acaagttatc cattttccca tctcatctaa actagaattt atgtggatca
     5401 tttttatctg tttgaattta ctatagtaac cataaatttc tcccaaatct tcttctaaaa
     5461 ctagtatctt caattctcgt gcaattctaa aaggattgcg tgtttgatat agttttacca
     5521 gcttattaat tttgttatca atttgaggta aatacataaa caacgtactc cttttaatcg
     5581 cgatattttt taggtgtgta ttttttctta gcttctattt tagctattcg caaagcattc
     5641 tctaaagaaa taatgagagc ttcacgtgtc gcttcactca tttcattatc tttcttcgaa
     5701 aaagccattc catcagcatt agataaatcg tcgattaatg actgcaattc tttttggata
     5761 tcacgctcgt ctttttctgt taaatcatag tatctttttt tctcagtacg tcctagcaaa
     5821 taatctacag atacgtcgaa gtagtcagca atttcttgta acttttcggc agttggctgt
     5881 tttcctgcgt ttaaactata aaagtaattc tctccgtact tcaaatcttt agcgacttgt
     5941 tgaaggtttt tattcttgtt tttcgctagt tcttttattc tttcaaatgt tgtcattcca
     6001 acgttccttt ccaacacgaa aaaataattt acgaaaaaga gtaaaaatag ctttacaatt
     6061 ttacgatata gagttattat ttatccgtaa gttaatttga tagaaaaaag caaactaaaa
     6121 acacacctta tagcataaag tttggcgacc aagtgcaaat aaaaggtttt gttataggct
     6181 tatttctcta tgtattcatt ttactctata gtgtaaaaat gtcaatggaa attatatttt
     6241 ttctatcatt ttaacttaca aatttataaa aaaaggaagt gggttaatgt caaacttgga
     6301 taacggtcgt gcggcgatca aagagtttat gaaagagaat ggaataacta tggatgattt
     6361 ggctactgct tatggtcaca cgcgtgtacg aatgcaacaa attcttgatg gacactggag
     6421 tggaccaaag gcaaacagaa ctattctaga gatcatccgt gattacagaa ttcgatagga
     6481 ggcaagtcat gaatattcta agcgaagagt ttttgactcg tttaagaatt gcaattgttg
     6541 aagttgtaaa ggacgcactt agtcaacttt caaaaaagaa tttgtcagaa acacgatatt
     6601 taaaaaagat cgaagctaga aaatatgtgg gaggtgtaaa cgatcaaggt tttgagaagt
     6661 taatagctca cggtttaaaa gaaattcgta tagatggctt tttaagatat gacaaaaagg
     6721 acatcgatga actgatggct aaatacaaaa tttaaaggag gtcaacatgg gaaagtttaa
     6781 tcgaacatta gtattcagcg cgccactaat tatctacgct ttaggtctat ggggtagtag
     6841 acaagcatta atcggcacga tcgtttacat gctttggatt ttcattgggc ttgatgaagc
     6901 tgagtacaga gcgaaaaagc cagtcgggag ggactgacta aaaaagtcta atgtctataa
     6961 gaaaggtttg acaataaatg aaagaattat tttttttaat aactttcatc attttttcta
     7021 tcagtgtttt ctacattaac ctttgcgttg ctagatttgc tttgaaaagt tttaggaaca
     7081 gcaaacgaaa atagagctat aaaagatacg aatgtagtag ctacgttaaa ttgctcttta
     7141 gaaaacgtca atgtcattgg aatgataatt gatatcgatg agaaaatctt tgcgataata
     7201 actatccatt tttcaattat aaacattttt aaattaagca ccattagaca aaatgtagtt
     7261 cctatataga ggataaatga agatagaaat aaacatatta gtccagtttt aaaaaaagag
     7321 aagtaatcta tgtgggcaaa ttctgttaat atgctttttt gatttaacac aatcaaacta
     7381 ctgttggaca aaaatgacag taaggaaaga gttgctccga ttactaaata aaaaatagtt
     7441 gttaacaata ttatattatc aatttttctg ctcatgtttt caccccacca gaattatatc
     7501 aaaaaggaga gaagaaataa tgcaagaatt agtaattttg aaaaataaag aagctgtgac
     7561 tacgagctta caagttgcag aaagcttcga taaaaagcac agacatgttt tgacggctat
     7621 tgatgaacta aaagaggggg ttgccgaaaa ttgggcagac ctattttggg aagatactta
     7681 tgttcatcct caaaataaac aatcataccg aattatttat atgaatagag acggcttctc
     7741 tctattagca atgggcttca ctggtaagaa agctttaagt tttaaacttc aatatattga
     7801 ggccttcaac aagatggaaa aagaattaaa agaccaattg ccatcaattc cagcaactaa
     7861 acgagaaatg gcattacttg ctctagctgc aaacgaagaa acgaatgaaa gagtagacgc
     7921 tatcgagtct gatttaaacg atttgaagaa caatcaactt ctagctgaac cagattaccg
     7981 aacaatttct aatatggttc gcaacaaaat tagagttatt tgtaatcagc agcatttaaa
     8041 tagcaaagca aaagcagaat tgtttaaaga cttgaatggc ggaattaagc gaatcactgg
     8101 agcagtagct agaaatcgta tcaaggcaaa acagttcgat gatgttatcg attttattaa
     8161 caattggatg ccttctacag cgacgatgac aattattaag caaatggagt tgttagagga
     8221 tgaatagagc tgaagcgcta aaaataggcg taataattgc taatcgctgg tggagacaca
     8281 ataaaccaaa catcctaagc caacaacata ttgataagca aaaagcatgg caacaaataa
     8341 aaaagtgact cagccgacca aagcaatgag tcacaaagaa aatacatcta aggagatgtt
     8401 accacatgaa aaaagaactt tccactctag atcaatattt gactgatcct agttggggca
     8461 aatcgaatat caaggaaaca agcaatcgaa aaatcagacg taatcttttg acggatgaag
     8521 aactagcatg tgatcaagac gatttgggaa attttgtgag tatttgggat catttctatc
     8581 ttatccattt atcgaggaag tccaaaaaaa ctgaatatat ctatgtcatc gaagatggct
     8641 tgattgatgc gctagaagag tatgacagag ataacttgat tgatatctct tattacggat
     8701 caggtaagaa atacattgct gaaatggagg cagaatttga tgagtgaaaa ggaacaacct
     8761 ttgaaaaata gaagtgataa tacgcttttt aataccttat acaaaataaa tgtgaaagat
     8821 gttactgaaa aacgaaataa cctcacttat ctttcgtggg catgggcttg ggcagaagtt
     8881 agcaaagtgt gtgaggcagt agattatgaa atctatcatg atccagaaac atatctgccg
     8941 tatgtctttg ataagaaaac aggctatatg gtttttacca gtatcacagt caacggagta
     9001 aagcgtgaca tgtggttacc agtcatggat ggtgcaaaca aggcaatgaa agatgagcca
     9061 tatacctacg aagtcaatga ttatcagtgg aataacgaaa cgaagaaaaa agagattgtt
     9121 ggaaaaatcg aaaagcgagt tgaagcagca actatgtttg atatcaacaa aacgatcatg
     9181 cgctgtcttg taaaaaatct agcaatgttt gggctagggc tatatatatt tgctggcgaa
     9241 gatatgccag aagatgtctc gatgcttgaa ccagctactc aaagaagcaa aaagctattc
     9301 ttggatgctt tacaactggt tgctaacaag tacgaaaaat caattgatga agcaattgtt
     9361 gcattgactg atgcggcttc tataaccgct gatgacagta aatggaccaa gagagacttg
     9421 ggcattctaa aacgaggcgt taactggatt gaagatcagt acagagaaga aacaaaagag
     9481 aagtgatatg agtgtttaaa ccattgatcg attcatattc agcggttctg aaaaagttca
     9541 aaggaaaaga cataggtgca acgatcaatg aagaagtgaa cattgatcga ctaaagacga
     9601 tgtatgacgg atatgatggc gatcggatta ttgaagttcg atttattgat ccacgtcgat
     9661 ttactgtgca gcaacgaaac ttcatctatg cgctgatagg cgatattttc atcgatacag
     9721 gcatgccaac ggacttctgg aaggaattct tttacttccg ttttgaaggc gtcacagggc
     9781 gcaaaataag cctcaaagac gaatcgaata caactgtgag tgatgctaat gtcttagcaa
     9841 atatcatctt agatttcatc tttgaacatc atattccttt caaagaaggc tatgagattt
     9901 taccagcgaa tcaagaatat tacttctaca aatgcatcac aaaaagagtc tgctgcatct
     9961 gtggcaaaac aggagctgac atcgatcact ttgacaaagc gctaggaaga cgaaagcgca
    10021 aagaagttga tcattcagag tacacatttg cagcactctg caggattcat cacacggaga
    10081 aacacaatat aggtgtgatc aatttcaaaa ataaatatca aatcaaaggg atcaagttaa
    10141 accaggaaac aattaagaaa cttagaatag gagggtaaaa aatattgtct gacaacaaac
    10201 gctactacta tttaaaacta aaagagaatt ttttcgatag tgacgagatg gttctcttag
    10261 aaagtatgcc agatggctat atttattcta atattcttct caaactttat ttaagaagtc
    10321 taaaacacga aggtaagttg atgtttaatg acaggattcc atttaactct acaatgctcg
    10381 caactattac aagacattct gtaggagtcg tagaaaaagc ggtacaaatt ttccgtgatt
    10441 tgcagcttat tgacgtatta gataacggag caatttatat gtctgatata caaagtttca
    10501 ttggaaaatc ctcaactgaa gcagatagaa aaagagaata cagaaagaaa atagaagagg
    10561 caaaacggaa tttaataact ggaggacaag tgtcggacaa atgtccagac aaaactacac
    10621 cagagttaga gatagagata gagaaagata tagatataga taaagaagaa aagaaaggta
    10681 agtattctga cgaacactta cgccttgcta aaaaattgca aagtaattta actgaagatt
    10741 ttccaaaaga aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg
    10801 aagaaagaga taaagcgtct atagaagcga ttgagtatgt gatcaattgg ctacctacaa
    10861 atgaattttg gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc
    10921 tcaaattcga aatcaaagca gacaagaata atcataaaaa gcaaagtcaa aaactacagt
    10981 acagcaatcc tagtgaatat gacgacttgc caatttaaaa aggagatgca tcacatggaa
    11041 agcctagcaa atgctatgga gaaactaata agaagagtat tagtgcaaag cgggaaatgt
    11101 ccagaatgta gcgaaccttt gtatagttgg cgagctaaaa ataaggatgg ttcagaacgt
    11161 tgtaaaccaa catgcatgag ttgtggttat aaagcgttac gtgtgaaaga ggatatacag
    11221 accgaacgga tatataacga cagcttaaaa gcacgagcgt tgagtttttt tcaaaatggt
    11281 tcggtattaa cagataaaac tttgtttaaa tgcaaaatgg agaattatca cgtagtggac
    11341 caagaaacga aaattgcttt agaaagagct aaaagctttg taaatgatgt cttactgaac
    11401 catcctgcac atttcattct atcagggaaa tcaggaagcg gaaaaagcca cttgtcaatg
    11461 gcgacagctt gggaaatact tgagcgctca aattatgaca agaaaatact ttttataagc
    11521 tatcaagagt tattagagca aataaagttt tcttataaca atgctgaact gagaaaagaa
    11581 attgaaggat cgcttatagc cgatatcaaa acaactgatt tggtggtttt tgacgatatt
    11641 ggagctgaat taggtagcgg ggtatcaaat agtaggcagt ttacaaacaa cacgttaaac
    11701 acgctcttag aagccagaca gaacaaggca acgatcatca caacaaactt atccggtcct
    11761 gaactaagag aagcctacgg cgaaagaatt gtttctagaa tatttaagaa ttcagaaggt
    11821 tatgcgctga aattccaaca aacagcagac aagcgcataa aaccagtgaa aggtagtatc
    11881 gcatgaataa ataccgtaat aagaaaactg ttcatcgagg tatcaagttt gattctatcg
    11941 cggaagcaga gtattacgat ttagccttgt ggcaagcaga agcgaacggc tggaaagtaa
    12001 aacttcagga aagatttgag ctgatgccga aatttgaact agatggaaaa aagtatcgca
    12061 agatcgagta tattcccgac ttcacatttt ataaaaacgg caaacttgtc aaagtcgtag
    12121 atgtcaaagg aatgcagaca aaagacttta agatcaaggc aaagttgttc tgtcatcaat
    12181 atcaagtgcc gttgatatta gctaaaaaat atcggaatac gttcaaggaa gagcgttttt
    12241 aacgaggtgg tccatcatga caacagaaga agtgattcaa atgcgtattc gaagccttca
    12301 acgtgagatt gacgatctgg aacggataaa ggcagtgatg gtcaatgaaa cggcgagaaa
    12361 ggcaatcaat ttgcacatag tgaatttaag aagggaaatt cgtagattgg aggaatgagc
    12421 gtgggtaaga aagcagcaat gaaacgaatc attgaactga cacattctga gaattggcaa
    12481 gaagacaaag aaatagttgc agaagtccaa aagctcggta aatcaatgtg gactgaaaag
    12541 cctaaacgga aaacgccgag aaaaattgca atctggcatg gtgatcgaat tctagtaacg
    12601 ggtacagctg aacagttatc tgaaattact ggactgagca aaaacatcat ctgggataga
    12661 gctaggagct tatggattga ttcaaagggg cgacagttta ggtatgtgga ggagaaataa
    12721 tggatctcat tacacaatac agtgatatca tcctcaagaa aatcatgatg aagattcaga
    12781 aagataaaaa agcaaaagaa cgagcggaat tagttaagtt ggaaatggct gaaacaggag
    12841 caggagtgcg aagtagcagg cattggaaag cagcagcaaa cattgaattt tattacaacg
    12901 aaattcaaaa agggttcgat cagatgcgtg agctggatcg gcaaacaaat tggagcaaga
    12961 aacttcatca ggatcgtttc aaatttgtag aaaagtatag agagatactg aatgagtatt
    13021 tcgaggagga ctagatgttt aattcaacgt tagcgtcaat tcacttttta ttctatataa
    13081 cgatatttgt agctatatta attaatgcga taaaaagcgg ttttttaaca ccaatcggca
    13141 caattttaac attagcttca tttgttacaa gttgtattat ccaacttaaa tatataaagg
    13201 agaatgaata atggacgaac taatcacaaa agtagagcag tgggctaaag ataagggatt
    13261 ggatcaagct gattccagca agcaaatgtt aaaaacgatc gaagagatcg gggaagttgc
    13321 cgcttctcta gctagaaaag atgaacatgg tttaagagac ggaattggag atgtagtagt
    13381 aaccttgatt attttagcta tgcaaaatga tatggatttg tacgagtgtc tgaaccaagc
    13441 atacaacgaa atcaaaggac gcacaggaaa aatggtagat ggtgtattcg tgaagtcgag
    13501 tgatttggag gacagcaaat gatcagccta acaatatttt ggtgtgtaat tggcggctta
    13561 ctagcgatgt taggagcata cctcatttac gagagtgcaa aaggcaaaaa atatacagca
    13621 gtagggatta ttttgtttgt tgtcggaatt ttagttgcga ttggtgcaga aataaatctt
    13681 tatttaaacg gtacgacaga agagttgatg tggtttttat tgtggacaag gagataggag
    13741 gacagcgaaa tgggcgatat gggcgaatac tggaaagata ttaaaccgca tctaaaagaa
    13801 cgtagaaaac aacatgtcag aaagatgggc aattcagcaa ctaaaaatat tgaaaccttg
    13861 ggatttgaat ttactcatta tccaaacaat catcaatttg caattaatac ccataatgga
    13921 attattgact actggggaac tacaggaacg tggattgagc gcaaaacaaa aaaacgtggt
    13981 aaagggatac gtagtttgag gaaatatatt aatcaggagg acagcaaatg aactatacac
    14041 aacaagaact gactgattta tgtccaaaac atgtagctga gttcatcaat aatgaagtcc
    14101 tgcctaaata tgcagatggt ttaaatacag cagaaaacgt tacagatttt atgattaatg
    14161 atgctattga tcgtttgata tttttagaaa tagattgtat tgcttattat cgtctacacg
    14221 cagaagttgc tttgattgat ccgtatattg ctttaagcca aaatcgaaaa atactcgtag
    14281 cctatattga taccaaacag attttagaag gcagagatgt cgagatagag atcgatgagg
    14341 aggaagtggg atgaaagcat acacaatcaa gttttatgga aataaaacct atgtatacat
    14401 cctaagcggt ctcagacttg attttagagg ctattcaaaa gaagaccttt tagaatcctt
    14461 atatggttac ggatatttaa atgaagatga actaataagc ttagagaaat ttacaaatgc
    14521 ttggacgatt gaggaggaag cagaatgaag atttatgtag taaagttcgg gaataaattt
    14581 tatagaagtg atgaacgctc tataggggct aacacattat tcattgtaga catactccaa
    14641 agtgcgagat ggtttgatag cctcgaagaa gctaaccaag tttcacgacg acttggtgga
    14701 ttaacacaag tatatgaact ggtcactgtc gatcatgagg aggtagaaag atgaaattaa
    14761 aagacggatt ttacgctagt agtcatggta tcggcggttt aatgctagat atgccgacaa
    14821 agaatcctaa aaaacgtaag aaaccaaaat tcaaagtcgg tgacatggtt cgctgcgaag
    14881 cagaagggtt catctatcca tttcgtggat atgtagaaca cgtctataat cactcagcga
    14941 tcattcgtat tgaaaacacg atggaatgtg ataagtggtt agcgaaaagc aaagagaatt
    15001 tagctgtagc gagattggtg gatatgaagg ctatataaac agcgaaaaaa tgtacttcta
    15061 agagaaaaat ttatttttta taaaaatagg aaagtatcat tatgaaaaag accacagaga
    15121 taaataaacc taccacataa aaaatgaata tttcttgcga ttcaatatag gtatatttaa
    15181 caatttgtac aataaaaaat actataggta aaaaagataa catgaggagc ttatttgttc
    15241 tagagtgttt gacaaagtaa caaaatgcga cgataccaat acaaacggga atccaaaata
    15301 acagcatgct ccacatagtc actcaaccat ctcctatacg ctttattatc aatagataat
    15361 atcatttttc gtaataaaga taaaaaaata agtaaggctt ctcattaaaa tataaaaaag
    15421 acagccgacc actggctgtc cttagcagaa tattgaaata gtgagccatc tgttttccgc
    15481 cagatagctc aacatgtata tgggtaacga gtgcaacctc gctacttgaa aagcttagca
    15541 taagcatggg tataaaaaaa gccggattcc tccgacctta attaataatt ctgacacaat
    15601 tattatatca taaatggagg aatcaatgga tggtactttt cgatgtaaag aagtatgaga
    15661 caccagaggc aaaagatgtg gatatggatc gcacaaaaca taatgttggt gtttttcttt
    15721 cagcatatct gtcagctaga tgtaggatag gtcaacctcg ggagcctaaa gtaacggcat
    15781 cttactcctt ggttccacct tctacagcaa ataatacatt tgaagcagaa cgaatgatga
    15841 ttgagagaga ggaagcgcaa gaagaatttg agtacttgca caaattattt gttcgtggct
    15901 attcagcaat tcagcatccg cataaacctg atgtaacaga aaggcgcaag aagatattct
    15961 atgatcgtta tatcaatggt ctgcccattt atgtaactgc tcaaaggaat aatactagcg
    16021 aagaatcggt taaagtagaa tcaaacagaa ttatcatcca atttgcttca tcgttagaac
    16081 tggttgcttt taagtagcca gtttttacac tttttatacc ctttgattac ccatttggtt
    16141 tccattttat acctttttta taccaatcac ttacctggta aatgatttat tatgatagtg
    16201 tcgaaagatt agtgataggt ctgagacaaa ataataataa aaggaacatc gttttattat
    16261 tgtttcacaa ttaagcttcg atagacagca acggaaatat taagaataag gatgtgaatt
    16321 ccaactcctt ctaaattgtt cttattatct atcatccgtt gctgtttatt aatttatgta
    16381 ttggagggaa aacgaatgga agtcgagcaa ataaagtatc aaggtatgga tcgggaacgg
    16441 ttgattgata ttattgagca acaacgttta gaattgatcc aaaaagaagc tgtatgtaaa
    16501 gagtacaaaa aacatttaga acaagtaatt gagtatcatt cagtagaaaa gtacagatca
    16561 gtggtgcaaa aaaatagagc aacgagtcct gatcctcgtc aagatgtatc gattagtatc
    16621 aagacgccag tatttaattc tgttaaatca gaaaagctag aaagtatctc aactgctttg
    16681 gtagaaaaag cagctgaaat gaactcccta gcaaatgaag ttagtcgtct tatttagtca
    16741 catcgataac ttgagagtct ttggtgattc cgttttttat agaattaatg gtttgttcag
    16801 cggaatattt agtcaagtat gtttcgctag ttgctactac ttcattgtta tcggacttaa
    16861 taacgaagta atattggcca ttagttgctt ctcttataac aaagtacaaa tttttcacca
    16921 ccttttaatt tatttcagcg gaccactcgc tgataaataa aattatacac ttagtattta
    16981 ttttcacaat attaatttgt cactgtggcg gaaagggtag acgctaagca tgtgtgttag
    17041 gtcaatgctt cggcaaccat gcaaggttcg attccttgcc agtgactttg gcaactgagg
    17101 gttggaaatg ggcgctcaaa gtacacgagc aaggcgaggt cgatagtaat cgatggaatc
    17161 ggtgtaggtt gcttgataga actataactg catctcgttt atgaggtgta gtttttacat
    17221 attagatcac tcatcgagtg gtctttttat tttggaaggg gagtaaaaaa atgaacgaaa
    17281 accaattaag agagttgttt aaaacgaatg aagcaaacaa aacaatggag gcgacattct
    17341 acgaaactca aaaaagctta gcgttaatcg caaaacaagc taagtatttc tacgatcagc
    17401 ttattctgca aggatttaat gacgcacagg ctatggaatt tatgatgcga accttttctg
    17461 ccagtaacca acagaaagag tgatacgtaa tgaggaacta ctggtatgta tcattatcaa
    17521 ataaatatcc gcaaccaaac gcagatgatc caattagagt tgtccagtca gtccagatta
    17581 aaaagaagta ctccatcatt gaaatgacca gagaagctac gccaaaagag attgataagt
    17641 acaatcttcg ttactgcggc catggatatt ttagtgagca gaacatacag acaaatattg
    17701 agagatattg ttaataaaat tgaaaggtgg tggcttgaat gtggtaaatt tgacaccaaa
    17761 acaaaaagct tttgcggatg aatatataaa aaatggtggt aatgctactc aagccgccat
    17821 taaagcaggt tatagcaaac gatcagcaag agtaataggt aaggaaaacc taactaaacc
    17881 taacataata cagtatatta atgaacggct aaatcctatc gaaaagaagc gcaaattaag
    17941 tgccgaggac gctttaaatg aattgataga tatttggcaa ggagaagtac aaataagcgt
    18001 gagcaagcaa atagaccgct tggagaaaaa caaggttatt aaacatatgc aatatgaata
    18061 tacaccagat ttagaaagca aattgaaagc cttggatttg tatttgaagt ataaatcgct
    18121 gttatcacaa acgcaattag aaaaagctca aacagaaata aaattaatgc aagcaaaatt
    18181 agaacaatta cagataaact cagagcgttc taccgaagaa aaacttgatg agttgttaga
    18241 aaagattagt ggtgaattag atggcactag ttgatattta taacccaaag caaatcgacg
    18301 tgttaaataa aaccattaag aatgattggt ttattacttt attacatgga gcaaaacgtt
    18361 ctggaaaaac aaaaataaac aatgatttgt tcttgtttga attgcgacgt gtgcgaaaga
    18421 ttgccgatga agaagacatt aaggaaccaa tgtatatcct agcaggagtt tcaagtgcaa
    18481 caatccaaaa gaacatctta caggaactat acaacatgta cagcatagaa cctaaattcg
    18541 ataaacatgg aaactttaag ctatttggcg ttaaggtcgt acaagcttat acaggaaaca
    18601 ttggcggtgt tggcgcaatt cgtggtatga cagcatatgg cgcttatatc aatgaggcat
    18661 cgctagctag acaagaagta tttgctgaaa tcgtttcacg ttgttcagga acaggagcga
    18721 gaatactagc tgatacaaac cctgacaacc cggaacactg gctaaagaaa gagtatatcg
    18781 acaattcaag caaaaatatt caatcgttcc actttgaatt agatgataat acatttttat
    18841 ctgaacgata tcgtaacaat attaaagaat caacaccaag cggcatgttt tatgatcgtg
    18901 atatcaaagg tttatgggtt tctgcagaag gtgtcgttta tcgtgatttt gatgctagca
    18961 agcactacat ccagtcaaaa gacttaccac ctttgagcaa cttttattgt ggtgttgact
    19021 ggggatatga acactggggt tcaatcgtag ttataggtga aacggatgac ggaacagctt
    19081 atttaatcga agaacatgct actcaatttg aagagattga ttattgggta gatgtggcaa
    19141 aaggcattca agagcgttac ggcttacgag taccttttta ttgtgactct gcgagaccgg
    19201 agcatgtggc taggtttgta agagaaaaga ttaatgctaa aaatgctcat aaagcacggt
    19261 tatctggagt tgaggaagtc gccaagagat ttaaacaaga taaattgttt atctgtcaag
    19321 atagagtgat gaaatttcgg gatgaaattt atcaatacat ttgggacaag aaaaaaggcg
    19381 agccaataaa agaatatgat gatgtgctag actccgttcg atatgcgata tatactcatg
    19441 agcttcttaa gaaaccaaaa gttaatgtca acgaaaagat taaacgtatt aaacgcatgt
    19501 tttaaggagt gtgaaaaatg gataaggtaa acgagtttga atacggagct gatatacatt
    19561 attctaatga cgtgaacaca aattatgtaa agtttagcgt agactcaaat cttcactata
    19621 gatttagctc agcagaagat ttacttaacg atttagatac tttagcagca atgataaaac
    19681 atcatcatga atatcaggta aaaaggctaa gtgtattaga tgattattat aaagctagaa
    19741 atacaaatat catggataac cgtaggcgca gagaaaaaga aaaagcggat caccgatcag
    19801 cacataactt tggaaaagtt ctttgtacgt ttgatgttgg gtacaacaca ggcaatccta
    19861 taaaagtgca aatcgaggac acaaatcaac aaaaagaaat cgaagagttt aatactaata
    19921 atgacataga tgggttaaat gctgaactct ggcttgatat ggataagtat gggagagcct
    19981 atgagattat ctatcgagat tcagatgata cagattatgt tgatttggct aatgtatttg
    20041 aaacgtttgt tgtatatgat actacagtaa aacgagagcc tattttggct gtacggtatc
    20101 ctaagacaag attcaacaag gatgctgata aacagtacat tcaaccaatc gtatacacaa
    20161 aagaaaaaag tatcacttat gatgagacga cactaacagc aattgagtta aagaatcccc
    20221 aggatgaacc gcatgaatat aaagaggtac ctattacaga gtattctcct aatcgttttc
    20281 ggatgggctt gtatgaagat gtactatctt tgattgatct atacgatgca gggcagtctg
    20341 ataccgccaa ttatatgact gatctaaacg atgctcttct agttattagt ggtgatattg
    20401 aagcagcagg actatccaca gaggacgcca tcaagcagaa agaagcgaat atgcttttgc
    20461 ttgagtctgg aactgatgtg aacggtaata aaacaagtgt gactgcagga tatatttaca
    20521 aacaatatga tgtgaacggt gtagaagcat acaaatacag agtacgcaag gatattcacg
    20581 aaatatccat ggttcctgat cttactgatg acaatttttc cggagtgcaa tcgggagaag
    20641 caatgaaata taaattattt ggatttgaac aaatgacggc aacaaagcaa aggctattca
    20701 aaaaaggcct tatgcggcgt tatcgtcttt tatttagcct aaaatcaagt atttctgaaa
    20761 tggataactc cgatttgaaa ggcttacgtg taatatttac gcctaatcta cctaaagcca
    20821 ttctggaaga gttgaaatct ttggttgatg ctggagctga actcagtcaa gagacgatct
    20881 taggactcgc ttcttttgtt ccagatgtac aggcagagtt gaaacgagta aataaagaaa
    20941 cgcaaaagca gattggcatt tttgattcag atggtgaaga agtaattaac aacaaaaaag
    21001 atgaaacagg ggggtgatta aatgaactcc caagaatatt ggatcaaacg ggaaaaggaa
    21061 tggcaaaagc aacaaattaa agatgataaa aagcgcatgg cagaaattaa aagtcgcatg
    21121 caatacgcac aagatgcgat acaaaaagaa atagacgcgc agtgggacag tttctccaat
    21181 ggccagaaaa tcactcgtag cgaagcgatg aagcgtgcta gtgaaatgga tgtcaaagca
    21241 ttcgctcgca aagcaaagaa gtatgtcaaa gagaaagatt tttctcctac agcaaaccaa
    21301 gaattaaagc tatacaatct tacgatgcgt gtaaatagat tagagctctt aaaagctaat
    21361 atcgggcttg aattgatttc actgtttaat gaattggata agtacttttc gaatgaatta
    21421 acaaaagctg gtttagctga attgaagaga caagccggta ttttagaaat gactattgct
    21481 tcaagtggat atgcaaagct gatagaacta gtaataaaca gctccttttt gagtgatgac
    21541 gtgtctttta gtgatcgctt atggatgtat caatctgaat tgaaatcaga attagatagg
    21601 ttgttagtca gaagtataac gatggggaaa aatcccaagc aacttgcatc taaattggca
    21661 gaatatttaa cagctgaagg acgagaaaac actaagttca acactcaacg tttgatggtg
    21721 actgaaacga ctagagttca ggtagggatt caagaacgaa gttatagaga tgcaggcatt
    21781 acccagtaca tctatatagc agaaccaacg gcgtgcaaac tatgtatacc gttaaataat
    21841 caagtttttg atgttgccga tatgcagcca ggaagtaacg ctcctaatat gcatccattt
    21901 tgtcgatgca gtacagcacc ttatatagaa cgaatatcaa gccgttaaca caaattaacg
    21961 gctttttatt gtgccttctt acagcttaca ggcgttaaag agaaagctat tttcggctga
    22021 ccggcgtaac tggtcaaatt tatcgggtag cggcgtaacc gtggaggatt aatcatgaaa
    22081 aaacgtttat ttatgccaat gaacttacaa tttttttctg aaccaggaga tggtggatct
    22141 ggtgatgagg gacaacaagg aaacctacca gctggctcac aagagacacc gcccgaagca
    22201 aaagaagaaa acaatactgg caaaacattt tctcgtgatg aagtagcgaa aatgatcgct
    22261 gctgagacga ataaagcaaa agcagcgtgg gaaaaagaac tagcagcaaa aaaagaagaa
    22321 gctaaaaagc tggcaaaaat gaatgcggaa gaaaaactac agcatgagtt ggagcaaaaa
    22381 gaagctgaaa tcgctgaatt aaagcgtgga caggcactat ctgaaatgac gaaagaagct
    22441 tctaaaatgc tgacagatgc aaatttacca cacgatgatg atttacttgg tctgattgtt
    22501 tctgatgatg cagatgccac aaaacaagct gtagcagtca tcactaactt tgcttctttg
    22561 attaagagag aaaacgcaag acaaacacca ccaaatgaag gtggacaatt tacagcatcg
    22621 aaaaatacta aagaaacagt ggctaaacta gctgctaaaa atcgaattat caaataggag
    22681 gaaaacttaa tgaaaaagaa acaactttta ccaatgaact tgcaaatgtt tgctcaaaca
    22741 tgggatccag ataatgtctt ggtatatgaa acgaaagagg gaaaaattcc tgataaatat
    22801 aatacgctca ttttgagtga agttatggaa aattctaaga tcatgcagtt agcaaaatac
    22861 gaagaaatga ctgacaaaga aaagaaattt gaatactttg cagaaggacc aggcgcatac
    22921 tgggtgggtg aaggtgaaaa aattaaaacg tctaaaccta aatggatgca agctacgatg
    22981 actgcaaaaa aactcggtgt cattcttccg gtttctcgtg aatatttaaa ttataaatta
    23041 tcagatttct ttgaggagat gcagccaaaa attgctgaag ctttctataa aaaatttgat
    23101 gcagctgcct tattaaataa agaaaaccca tttcctcagt cactagacgg atcagttatt
    23161 agtgcgggga atgtggttga aggcggattg acttatgata atatcctagc cttagaagac
    23221 aagttagcag aaaatgaatt cgaacctaat gcgtttattt caaaccgaaa aaatcgtaca
    23281 gaattacgtt ctgcagctca aacagtcggg tcaaatgttg agtttattta tgatcgctct
    23341 actaatacaa ttgacggatt accagtagta gaccttaagt ctttagataa aggaacactt
    23401 tacgctggag actttaatta catgttttat gggatcccat ataatatttc atttaagatt
    23461 tctgaagaag cccaattgtc tactttaact aatgaagatg gaaccccagt taacttgttt
    23521 gagcaagaac tgattgcttt gcgtgcaaca atggatgttg gatttatgat tgtaaaagat
    23581 gaagcatttg ggaagatttc cccaaaagcg taacgcctgc taccggtatt gtgccaaatc
    23641 aaaagacatg gaccggtaaa gtaggcgata ctaaaacatt tactatttca gctgtgcctg
    23701 cagatgctag cgatgcagct gctgttgttg cagctactac agcaacttca agtgatggag
    23761 ctatcgcaac agtgaccaaa aatgaaaatg gtggttttga tggaacgatt gcagcagaag
    23821 ggtcagcaac attcacattt acttctggag aattcactac ttcaatcaat gtgacaggtc
    23881 aacctgctag ttaggaagta aaaatatgat gattgcagat gatattaaaa aacttcttaa
    23941 aggaacaata gatgaaaagc ttgaagttat tgagcgaaga acgaatgagc gtatgaaaac
    24001 cttgttaaat acgcaagaag ttcctaaaga atttgaaaca gttgtatatg aagtatcgtt
    24061 gaaaagattc aatagaattg gtcaagaagg tatgcagtca tattctcaag aaggtttatc
    24121 tatggctttt cctgactcgg atttttcaga gtatcaaaat gagattgatg aatttaagcg
    24181 taaagatcag gaagagttgt acaagccaaa gcgagggagg tttaaatttg tatgagattt
    24241 acagatgaaa ttatatttgt taaacgttca tctgactcta aatatgatcc agatctcggt
    24301 gagtgggttg aaggcaaacc agaaagaaca agaacagagg caaacgtgac agatattggc
    24361 actgatagaa gtgtgactat ttttggtagt gtggaagaag gggcgaaggt cattaggacg
    24421 cagcctcttt tttctatccc tacatttgac tatatcgaga ttgaaggaaa gacttggcaa
    24481 caaaaaacag ctagaaatcc agcatataga aatagtttaa ttgtgcaaga ggtggttctt
    24541 gatgaaggca caacttgaat ataaaggaat cgatcagctg atgcgacatc tgaaaaaagc
    24601 agcaacgctt aatgacgttc aaaaagtcgt gaaaagtaat actgctgaaa tgactgaacg
    24661 aatgcaaaaa ggtgcgccag tggatacagg ttacttacga agatcaataa acatgaatct
    24721 tttagaagct ggtttaactg gtattgtagg accgacagca gattatgcac cttatgtaga
    24781 atatggcact cgatttatgt cggcacagcc ttatgttaga ccagcgttta attaccaaaa
    24841 agtcaaattt atggctgaaa tgaaagcctt ggtgaaatga tgattaagac aagagatcaa
    24901 tcaatttttg atgaactttt taaaatatcc caaaacaaac ttggatataa aacatacgat
    24961 tacaaaactt tagaggatgt tggttatcct tttgtggaat ttgagaacac tcagacaatc
    25021 catgaagtaa ataaaactga cattaaaggg tctgtgattg tggttttatc cgtttgggga
    25081 ttacagaaga aacgaaagca gttgtcagat atggcatctg ctctttttaa tgaagctaga
    25141 ttgatagaag ccacagaagg ctattattgg gctttaaatt atcaagcaag tggaattcaa
    25201 gtgatggacg acacaacaac gaatacaccg ctaaaacggg cggttgtcac acttgaattt
    25261 agaattagat aggaggaaga acatggaagc attaaaaggt attgatgtca ttttgcttta
    25321 tcgcttattg aaaaaagaaa ctcaggaagc cgcttggaaa atggcatttc aaacagaaca
    25381 cgaaaatggt ttatcaagag attcagactc tacagtgaca aaagatggaa acgttcaaag
    25441 tttaagcccg gttgaatatg atttttcggc tacttcaata gttgctaaag gagattctca
    25501 tgtagatgaa atgaaacaag ctttattaaa tggggatatc attgaaattt gggaaattaa
    25561 taaagcagaa cagggaacag atgataatgc aaataagtac aaagctactt attaccaagc
    25621 atatgtgtct gaatttactc catcggctgc tgcagaggat aacgttgaat taagtttatc
    25681 atttgcagta aatggtgttg gtcaagatgg ttatgcaacc ttgacagaag atcaagccga
    25741 tgttgttcaa tatgcattca aagataccgt gaaagcaact tcgacaggag cataagaggg
    25801 cttagatgct ctctttttta ttttaggagg atgaaaaaca ttgaaattaa aaattaaagg
    25861 taaagaatat tcgtttaaat ttggcactaa atttgtacgt gaattagata aagtgatgcc
    25921 tttcgtcgat ggaaatatgg aatttggaat gggactctcc gcaaaagtct taccagagct
    25981 acgttcttat aacgttaaca cgttatcacg agttctggaa atagcaaata gaactgaaga
    26041 tgaatctatt acattagatg aattggatga ttacatcgat gaagttaaag acattgaaaa
    26101 attgtttgat gaagtcctaa aagaattggc ggagtcgaac gcgggaaagt tagcggtccg
    26161 aaacctgaat cagaaattga aagaagcgga aaaacaacaa gtggaataga ttctgcactg
    26221 gcatacgaac aaattcttat caattctttt cgatatttgg gaatgaccaa tatctcagat
    26281 atcgaaagaa tgacgttata tgaatacaac attcgtatga ctgcagccca gttatcttgg
    26341 cttgacaaag aaaagttgat tcacgaatta gcgtgggcaa atcagcaagt ccaagcggag
    26401 aaaaaagtag gcaaaaagac agttcctgta tatcgatcct ttgaagaatt cttcaattat
    26461 caaaaaatcg aagattcaat catgggagtt tccgaacttt caaaacaaga taaaaaattc
    26521 caaagcttac taactaaagc taactcttga ggaaaggagg agaatcatgg aacaattttc
    26581 tgttgaagcc ttattaaaag ccacagatag tggatttgta aagactttta aagatgcaca
    26641 agatgctgtt aaaacttttg aaaagaattc aaatagtatg acaaccgctg ttggtaaagt
    26701 gatgcaaggt actggtgccg caatgacaaa gtatattacc actcctctta taggagtagg
    26761 cgtagcagct gctaaagttg gtggcgactt cgaagcacaa atgagtcgtg taaaagctat
    26821 atcaggagca accggcgaca cattcgaaca gatgaaacag caagcgattg atctaggagc
    26881 aaaaactgct tttagcgcaa aagaatcagc tgctggaatg gaaaacttag cttctgctgg
    26941 atttagcgca caagaaatca tgaaagcaat gccgggtctt ttagacttag cagctgtatc
    27001 tggaggggat gtggctctag cttctgaaaa tactgctact gctttgagag gatttggttt
    27061 agaagcaagt gaagcaggac atgtcgctga tgtatttgct cgtgctgctg cggataccaa
    27121 tgctgaagtt ggagacatgg gagaagcatt gaagtatgtt gctcctgtag ctaattcaat
    27181 ggggatttct ttggaagaaa ctgcagcagc tattggtatt atgagtgacg caggcattaa
    27241 gggttctcaa gcaggtacaa cgttgcgagg agcattgtct aggttagcaa ggccaacaaa
    27301 ggctatgcaa gatacaatgg ataatttagg tgtttcgttt tatgatgctg acggtaaaat
    27361 gaaaccttta aaaactcaag tagaattact taaaaaagct tttgaaggcc tgacgcctga
    27421 acaacaacaa aatgctttag taacactata tgggcaagaa tcattatcag ggatgatggc
    27481 tttgattgat aaaggacctg attcattggg caaattaaca aaatctctga aagattctga
    27541 tggtgcagct gacgatatgg ctcggaccat gcaagataat atgaattctt ccatcgagca
    27601 aatgtttgga gcttttgagt cagcagctat tgtaattcaa aagattctag caccatccat
    27661 caaaaaagta gcagatgcca tatccggctt agtagaaaaa tttgtaagtg ctccagaatc
    27721 aactcaaaaa ttagtagtgg ccataggagc aattgtcgct gctataggac cgttaatttt
    27781 tatgattggt tcagtaatta tatggatcaa tagggtgaaa gtagctttta aagctttaag
    27841 tgaaagttca aaattgttta gtggattaag taaagcaatg ggtcttctta caaatccggt
    27901 ttttctggtt atagctgcgg tagcactact cgttgtaggt ttcatctatc tttggaatac
    27961 gagtgaagat tttagaaact tttggattgg cttatgggag ggaatcaagt ctgctgtaag
    28021 ctcggcagta gaatggattc agaatgcatg gaaatctaca ggagaatggt ttaacaattt
    28081 atggaagtcc attaaagaag gcgcagacaa tgtttggact acagttcaag aagcccctgg
    28141 gaaagcggca gattggatta aaaacaaatg gactggaaca aaagagttct tttcaaattt
    28201 atggtcaagt attgcaaact ctgcttcaga gatttggaat agtttaaaag aaggtgtcat
    28261 atcagttatt gatgatttag tttcaagtgc tggtgaaaaa tgggaagggt ttaaaaatac
    28321 tatatctact gcatggaaaa caattacaag taaaatcaaa tctggttttg attttatact
    28381 aaaatatatt ggtccatttg taagtagctt ttcagatgtg ttctctaata tagtgaaagc
    28441 aataacaagt atatttgctg aggttaaaaa cataatagta aatgcttggg aaatcattaa
    28501 gtctttaata gctgcgccac tactgtttat tatagattta attactggtg acttcgaaca
    28561 aatgaaagag gatttagatc taatctggaa cacacttgtc caatcagtgg taaatatttg
    28621 gacatctgta aaaaatatat ttacggaata tatcggtgca atagtaaata gtgccgttag
    28681 tttatggact ggattcatac aaagtatttc taatatttgg aatgaagtag tttatcaagc
    28741 gactatgatt tggattgatt tgaaactatt ttttactaat ttatggattg atattaaata
    28801 cagtgcaatt caaatgtgga taaatctaaa attctccata attcaaactt ggattgatac
    28861 aaaatatggt gcaattgaac tttggaataa tctaaaacaa tggtttttcc aaacggttaa
    28921 taatatcgtg caaactctta taaaaagttg gaacagctta aagcaaggaa cgatagattt
    28981 attcaacaat acagttcaag gtgctaaaga catttggact tcattcaaat cttggattgg
    29041 tgatttaatt actggaacca aagataacat cattcaggga tgggaaaacc taaaacaagg
    29101 tactatagat actttcaaca atttagtaaa tggtgctcaa gaggcatggg ataatttagt
    29161 aaatgctgtt agtgacacgg ttgatagagt aactggctgg tttgataact tgaaaaatat
    29221 cgatttacta gcagccggaa aagctatcat ggatagtttt ctagaagggt tacaaaatgc
    29281 atggaaatct gtgcaagatt ttgttggagg tattggtgat tggattcgtg aacacaaagg
    29341 acctatccaa tacgatagaa agctattgat tccagctggt caggctatta tgaacggtct
    29401 gaataaaggt ctgacaggag gattcaatga cgtacagaat actgttggaa gtatggcaga
    29461 ctttatcgcg gaacttttca atgcaaattc tgatgtagat atagctgcaa atttgaaaaa
    29521 tgcaaataaa aacattggtg cacaagttga acataaaata aatatgggtg gctctactaa
    29581 accagctgta tttaaaatca atcttggaag acaatcgttt agattgttta tggacgatat
    29641 ttcacaagct atgggcgaag gtgcagatat taatctggaa ttttaggagg gaatattttg
    29701 gatcaacgag aaaataaaat gtactcattc aaagatacaa ccattaatct cactagttct
    29761 aaacgattcc ttccgacgtc tgccatgatg tacgatggaa tgtatttaga agatttgatt
    29821 gatggttatc aaacacttac ggtggaaggt agagaaatgc tttctgtaga agttgaacag
    29881 caagagatac aaattggttc aatcattaca aatcagaaaa taccttcaag aacactaaaa
    29941 ataacatata agctggaaga tagagatcca gaaaaactac agtttaaatt caaagaactg
    30001 ttgaattatt tataccggaa tgaagacgtg gaaattaggt ttcatgatga attagattat
    30061 tattactacg gtcgctatac atcaactgat actgttccag gagactccaa ctcgattatt
    30121 tcgagtttta atgtattctg tgcggatcca ctaaagtata cgaaagagtg tgttagtgat
    30181 ggctatattg gaaatccgat acagtttcct ataacaccaa gaaaaattga agttacttta
    30241 tccatgaata attcaatcaa aattacaaac ggagaacaaa atatcacgat aactgacgcg
    30301 gcaataaaaa caggagacgt gttggttttt gatttttccg atgagcaggt aactgtaaac
    30361 ggagaagatt gtacttctat gattgattta gaaagtgatt ttgagaactt ttatcttaag
    30421 caaggtcaga agataactag caataatggg aagcttaaaa tattctatag gggggcgaca
    30481 atttgagtga gacagtttat ttctttgatc acttgcaaaa acttattaaa agaaaaaata
    30541 caagaagttt gattgaagtc tcccaagaaa aagaaattag ttctgataag agtgatctaa
    30601 tgaaagatac tctttacgtt acgacaaaat atgataaaga aatagaggat gcaagatata
    30661 tggcgattcg tgaaaacgag tcgtcttttt cgttgtatcg aattactaaa gttagcgacc
    30721 catttgaaac attagagttt acagggttag gatttgcgac aaatgaatta gatgcttaca
    30781 tcatcaaaga tattaggccg agtgggcagc ccttaaaaaa tgttcttgat cgattgattg
    30841 aatttactga aggaaattgg cgcgttggtc acgtagaagc aatgttacca acagtaactg
    30901 caacttttta ctatgtctct gtaaaagaag cgttgaaaga attgcaaacc ttaggtatgg
    30961 aatttgtctt taggtgttct ttgaattctg atggaataaa ggataaatgg atcgaagtat
    31021 atgaacaaat tggcgaagaa tcgaatacac gtttcgtata tggtagtaaa gcattaacag
    31081 ttgtaagaga gatagataga agctcaatct caacttcaat gataggtcgt gggcgaggcg
    31141 aagaggttgg tgacggttac ggtagaagaa ttgaattcac tgatgttgaa tggaaaaagt
    31201 cgaatggtga tcctttagat aagcctaaag gccaaaattg gcttgaagat ccggaatcaa
    31261 ctcaaaagta tgggatacca caaaaagatg gatcaatgag aaaacgagaa accgtagtag
    31321 tgtttgatga tatagatgat ccaacagatt tacttaaaaa tacttattca accttaatcg
    31381 attctgctag accgttagta caattcaaag ctgaagtcac tggaggagat gtgataggaa
    31441 atacagtgac tattcacaga tacgataaag gttatcacta taaaactcgt atttataaaa
    31501 ctacattcaa tcggcttacc ggtcaaacga atatcgaact aggggataat ttaacacaag
    31561 atgttagaaa acaaacggct tctattgtca ataatattaa tagtttagaa tctagcaaaa
    31621 tgacatttta cgaatcgaca gagattggaa aatatcaaga tgacattatg cgaggtgcag
    31681 gagataatgg cggttctatt tattgggtaa atggaattga agctggtgtt agtgatagta
    31741 gagaaatcta tgaaactgtt tatatggatg gacctaacat tcctagatca cgctttttta
    31801 tggtccaaaa taactcaggg atatctttca aacaatgtaa aaaaggtgag tggcaaacaa
    31861 ttcaagatgt acacaatggt gatagcacga ccgcttggac gttggatgga actttcaatg
    31921 ctaattttat tcgcgcaggc attctgtctg gtatcctcgt gcaaggggta gctttaaaaa
    31981 catttgacga taaagatttc caattagtgg cagagggggg agaactttct tttgaaaaac
    32041 aagtcaagtc tactggacta gatgatgtac atggagaacg cttgggctca attgtatcta
    32101 cttatggttc taaagggatt aacggctttg ctgtctggaa agagccaaac tatatttttt
    32161 ccattaatgc tggggacggt ggcgatcgag ggaatcctat ttttcaaatt ccagcagatg
    32221 ttactgctga taagcgcaaa tataatcttt atggtgacgg caatttttca gaaggaaata
    32281 taaccataga tggacggcta gatgtaaaag aactctatgt taacggtaca aaaattgaca
    32341 ctaatggagg aggcaattcc ggagggaacg ataacggttg gaacggacaa tacccaccag
    32401 aagtaactac tgatcgggat aaacgatatt ggcaaatttg ggcaacggca ataggtgccg
    32461 gttttacaca acaagctgct gcagccttac ttggaaatgc acaaggagaa tcagatgcta
    32521 atccaaccgc tgatgagggc aatggcgcac cagggttcgg ttatggtgta tggcaatgga
    32581 ccgattcttc tggcgcaact agcggacgtg tctacatgat caatttaatg acaaaggctg
    32641 gcatcagtga tgatccagac acaatcacgg cacagtttaa attgttgatg tggcatgcac
    32701 caaatggtca atggatcgca actagcgctt atccttacac atggacacaa ttcatgaatc
    32761 tgaccgatat caacacagca gcacaagcat tcgtggctaa ctttgaacgt ccacgtgatc
    32821 cacatccaga acggacggca tggtcacaag aatggtatga caagttcaaa gatttggaaa
    32881 ttcctgtatc aaaaggatat atgaagccga ttgcggatcc aatcacagtg acgagcgaat
    32941 ttggctggcg cacttctcca atcacaggag cacaagaatt tcataacggt attgaccttg
    33001 taaatggaaa tcctaataca cctatttttg cttcagcgga tggcgaagtg attgttgcag
    33061 gtgatgcaaa ttactttgac tggtatggaa attggacagt gattaaacac gctgatggaa
    33121 tgtatacagg atacgcgcat caaagccgag tagacgtctc aaaaggtcag aaggtaactg
    33181 ctggtcagca aattggactg atgggggcaa caggaccatc cactggagaa catcttcatt
    33241 ttcaatttat ggatgagttt tatccatctt cttcaggtca tttccacaat gcaagagact
    33301 acatcgattt ctaaaggagg gatagtcatg gcagaaacgc agcataaaat ggtcctatcc
    33361 accaccgaac caaacaacgg aataaatttg gttcgaattc ggcaagggga tgttttaacg
    33421 caaaagttcg ttgttgaagt ggtggaacat ggcaaactaa aaacattcga gggcctagtg
    33481 ccgtttttta ttaatacaac aaaatttggc gaaaaccaac ctgttgaaca aaaagtacaa
    33541 gaatacagtc cagcgcaagc aaggcttgtt tacaccttaa gtgagcctga ctggcaatgg
    33601 ggtggtgaaa acaccgcaca tttcagtttc cgatcactta atggtgatgg aacttggagt
    33661 gaacaattta gcacacagga ttttacctat cgagtcattt ctggaatatc tagaagccag
    33721 ttacgtgact ctggctatgt gtggacattt gaggatttgc taagaaaatt caaagattac
    33781 atggatcagg gcaaaaatga ctgggagcag tggttagaag ataatcgtga aatactggaa
    33841 aatatcgatc caggtggtat gatcattaac attctgaatg aagctaaagg cgactacgaa
    33901 tcattagctg atcgtttaaa ccaaaaatac caagtgccag tcggcagctc acaaattaga
    33961 gaaacaacac gcttttttga ttacgacacg atgaaatacg ttgacctagt gccgcgcaat
    34021 ttgaatacgg ttgtcaacag tgttaataac agcaaattta acttttcttt cattacagac
    34081 attcacgcag ataatcacaa cttgcgtata gatggcgtcg gttacaaaga tgcttcttat
    34141 ttaagacatt ggcgcgcaat ccctcaattt caaaaattag ggaacaaaac agatgtgatg
    34201 atttacggcg gagacaatat cgacggcgga cttggttcac tcggcagcga tataggcatt
    34261 attgatgaat ggagcgcgcg acattccatg ttaggcacgc tcaaacgctt cactaacgca
    34321 gcggtagcag gacaagaaaa accggttatt atctgcaaag ggaatcacga cgcttgtttc
    34381 gaccccgcgt ggcgcaagcg aaagggaatg ttatgcaacg ctgacttcga agagtattgg
    34441 aacggtttgt acggtggcgt gttgttccca gataaaaacg cagcaattta ccgtttcgat
    34501 acttgtgatt tttatgaagg cggcacaggc gacaagtacg tggacggtta cagcgataca
    34561 gcgccgggag ctttcagtgc caaacaaatt aacgctttcg gagaatggtt agtgaacgtt
    34621 ccgagaaact atcatgtggc gttagtagga catacgcctt taggtctcag caagtttccc
    34681 gttcgcaatg aaaacatgat tagcacgttg atagaagggt ttaaatctgg tagtccggta
    34741 actatcgatt ggtctaaatt aggacaaccg aatgacggtt cgttcggcgg attaaagacc
    34801 ttcgcaatga atacaaaagg cgccggcgtt gtagtaggtt atttctgcgg gcattggcac
    34861 gaacaagtcg aaggggcgtt tggaacagta aaaatgattc tttgcgacgt cggcttctct
    34921 catacagcta gccaagttga tacaccggat gaattagcgt tttataaaat agaagttgac
    34981 acagcaacaa gaaaagtgac aagcaaaggc gtagggcgcg cgagagactt cacttataac
    35041 tattaaggcg gtgaacaatt aaatgttaga ttttcaaagc aaaccaaata tttttgaaga
    35101 aatgagttat gaagaggctg tgaaatggtt attgcgtcaa gcggctatcc attacgacgg
    35161 ctcggatcac gacgcgcacg tcctagctac tgaaagcaac gcgggctttg ctacaccgga
    35221 aacagttatg caagcgcgtg ggcgttggtt acgcgattat aagctaccac aaaaatatcc
    35281 gaacatttta gacattccgc ccggcaaata cgcaaccaaa gccggatggg gcgcagacaa
    35341 ccctgacggg attgaagatg acagttttgt tgaaatgatg gtattcgcgg atcacgattc
    35401 gagaaaatta atcgtcgctt ttgctcgtta tagcggcgaa atttacatca aaatgacaca
    35461 taacagcgaa ccggtagagg gttataactc gttaggctgg cggcgcgttt acactacttc
    35521 tgttcttttt gaaggcgaat taagaaaagg acaatcagtc aatctgccag atgatacttt
    35581 ccgttaccaa acgcttcgta tccactacac agacggtgac ggagactttg tagaagaagt
    35641 gaaacgtcaa agatacgcgc gaattacaaa agcgaattta tggaatggag acgcgggagt
    35701 gacgttcatc gaatttgagc tgacaatcga agcgcgaaag ataacgatgt caaacggcag
    35761 agctttagat atttcatccg gcaatgtttc caatcccgca atgagcaaca acgtgaaaat
    35821 aactaagatt gaaggtgtga aataatggcg catgtcatca aaaaaggctc tatcaaagta
    35881 cctacacagc cgaaagacta cgatttgcaa gcaacggggc ttgtatttaa atcatacgat
    35941 aatcaaatag cgttagagtt caacgtcgaa caacagaacg gcacaccggc ggacttgcta
    36001 ggagctaact tgcgcttgtt gatgtttatc tatgatgaag tagatggaat gatcacgaaa
    36061 gagccaatcc cttttatcac gaaaaacctc atcactgaaa gcttcttgaa tggacaggtc
    36121 gtatatatct tgccagaagc gatgaaagct tataacggta tggtggaagc ttatgtttac
    36181 atcgagtatc cagacggatc aacaagtgat aacttaggct tcaccttccg tatgaagcgt
    36241 tcagcaatcg acggactagc gcaagataaa gcagactact ttattgcaga ctttcaacaa
    36301 ttacttgatg gagtcaaaca agaagcaaca gatgctgtta acgcagcatt aacaaatgtg
    36361 gattctacga ttgaaaaggc aaaccaacaa ataaatgaat ttgtagaggg agccacacaa
    36421 gcaattgatc aaactgttga cgaggtaaca gagcaactac aagctactca aactaagatt
    36481 gataccgttt ctcaaaacgt tacatcggca caaaacaatc ttaaagcagt tgaagacaag
    36541 atgaatcaaa ccaatcagca aatcggcgac ctcggcaagc tgaaaa
//
LOCUS       DAYLZO010000011        36515 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.011, whole genome shotgun sequence.
ACCESSION   DAYLZO010000011 DAYLZO010000000
VERSION     DAYLZO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.011"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..648
                     /locus_tag="ACGVJG_000555"
     CDS             <1..648
                     /locus_tag="ACGVJG_000555"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HFJ6556538.1"
                     /translation="TFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYH
                     FLGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTR
                     LELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLP
                     STIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            1364..1609
                     /locus_tag="ACGVJG_000556"
     CDS             1364..1609
                     /locus_tag="ACGVJG_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556539.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            1831..3441
                     /locus_tag="ACGVJG_000557"
     CDS             1831..3441
                     /locus_tag="ACGVJG_000557"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFJ6556540.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(3418..3630)
                     /locus_tag="ACGVJG_000558"
     CDS             complement(3418..3630)
                     /locus_tag="ACGVJG_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556541.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            3733..4602
                     /locus_tag="ACGVJG_000559"
     CDS             3733..4602
                     /locus_tag="ACGVJG_000559"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFJ6556542.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            4767..6038
                     /locus_tag="ACGVJG_000560"
     CDS             4767..6038
                     /locus_tag="ACGVJG_000560"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFJ6556543.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            6035..7330
                     /gene="rho"
                     /locus_tag="ACGVJG_000561"
     CDS             6035..7330
                     /gene="rho"
                     /locus_tag="ACGVJG_000561"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFJ6556544.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            7429..7692
                     /locus_tag="ACGVJG_000562"
     CDS             7429..7692
                     /locus_tag="ACGVJG_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFJ6556545.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            8137..8586
                     /locus_tag="ACGVJG_000563"
     CDS             8137..8586
                     /locus_tag="ACGVJG_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFJ6556546.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            8643..10061
                     /locus_tag="ACGVJG_000564"
     CDS             8643..10061
                     /locus_tag="ACGVJG_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFJ6556547.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            10061..10921
                     /locus_tag="ACGVJG_000565"
     CDS             10061..10921
                     /locus_tag="ACGVJG_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFJ6556548.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            11095..11868
                     /locus_tag="ACGVJG_000566"
     CDS             11095..11868
                     /locus_tag="ACGVJG_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFJ6556549.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            12015..12380
                     /locus_tag="ACGVJG_000567"
     CDS             12015..12380
                     /locus_tag="ACGVJG_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFJ6556550.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(12528..13736)
                     /locus_tag="ACGVJG_000568"
     CDS             complement(12528..13736)
                     /locus_tag="ACGVJG_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFJ6556551.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(13755..14033)
                     /locus_tag="ACGVJG_000569"
     CDS             complement(13755..14033)
                     /locus_tag="ACGVJG_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556552.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            14226..14882
                     /locus_tag="ACGVJG_000570"
     CDS             14226..14882
                     /locus_tag="ACGVJG_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFJ6556553.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(15324..16121)
                     /locus_tag="ACGVJG_000571"
     CDS             complement(15324..16121)
                     /locus_tag="ACGVJG_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFJ6556554.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(16118..17302)
                     /locus_tag="ACGVJG_000572"
     CDS             complement(16118..17302)
                     /locus_tag="ACGVJG_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFJ6556555.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(17327..18574)
                     /locus_tag="ACGVJG_000573"
     CDS             complement(17327..18574)
                     /locus_tag="ACGVJG_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFJ6556556.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            18789..20534
                     /gene="dnaX"
                     /locus_tag="ACGVJG_000574"
     CDS             18789..20534
                     /gene="dnaX"
                     /locus_tag="ACGVJG_000574"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFJ6556557.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            20554..20868
                     /locus_tag="ACGVJG_000575"
     CDS             20554..20868
                     /locus_tag="ACGVJG_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFJ6556558.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            20893..21489
                     /gene="recR"
                     /locus_tag="ACGVJG_000576"
     CDS             20893..21489
                     /gene="recR"
                     /locus_tag="ACGVJG_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFJ6556559.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            21553..22197
                     /gene="tmk"
                     /locus_tag="ACGVJG_000577"
     CDS             21553..22197
                     /gene="tmk"
                     /locus_tag="ACGVJG_000577"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFJ6556560.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            22212..22541
                     /locus_tag="ACGVJG_000578"
     CDS             22212..22541
                     /locus_tag="ACGVJG_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFJ6556561.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            22555..23493
                     /gene="holB"
                     /locus_tag="ACGVJG_000579"
     CDS             22555..23493
                     /gene="holB"
                     /locus_tag="ACGVJG_000579"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFJ6556562.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            23529..24353
                     /locus_tag="ACGVJG_000580"
     CDS             23529..24353
                     /locus_tag="ACGVJG_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFJ6556563.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            24346..24693
                     /locus_tag="ACGVJG_000581"
     CDS             24346..24693
                     /locus_tag="ACGVJG_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFJ6556564.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            24762..25634
                     /gene="rsmI"
                     /locus_tag="ACGVJG_000582"
     CDS             24762..25634
                     /gene="rsmI"
                     /locus_tag="ACGVJG_000582"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFJ6556565.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            25743..26864
                     /gene="dinB"
                     /locus_tag="ACGVJG_000583"
     CDS             25743..26864
                     /gene="dinB"
                     /locus_tag="ACGVJG_000583"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFJ6556566.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(26918..27520)
                     /gene="nrdG"
                     /locus_tag="ACGVJG_000584"
     CDS             complement(26918..27520)
                     /gene="nrdG"
                     /locus_tag="ACGVJG_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFJ6556567.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(27835..30024)
                     /gene="nrdD"
                     /locus_tag="ACGVJG_000585"
     CDS             complement(27835..30024)
                     /gene="nrdD"
                     /locus_tag="ACGVJG_000585"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFJ6556568.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            30350..31117
                     /locus_tag="ACGVJG_000586"
     CDS             30350..31117
                     /locus_tag="ACGVJG_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556569.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            31107..33149
                     /locus_tag="ACGVJG_000587"
     CDS             31107..33149
                     /locus_tag="ACGVJG_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6556570.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            33430..33579
                     /locus_tag="ACGVJG_000588"
     CDS             33430..33579
                     /locus_tag="ACGVJG_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFJ6556571.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            33590..35104
                     /gene="dltA"
                     /locus_tag="ACGVJG_000589"
     CDS             33590..35104
                     /gene="dltA"
                     /locus_tag="ACGVJG_000589"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFJ6556572.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            35101..36306
                     /gene="dltB"
                     /locus_tag="ACGVJG_000590"
     CDS             35101..36306
                     /gene="dltB"
                     /locus_tag="ACGVJG_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFJ6556573.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            36351..>36515
                     /gene="dltC"
                     /locus_tag="ACGVJG_000591"
     CDS             36351..>36515
                     /gene="dltC"
                     /locus_tag="ACGVJG_000591"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFJ6556574.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVS"
BASE COUNT        11678 a         6440 c         7634 g        10763 t
ORIGIN      
        1 actttcatgc cgaaaccgtt atttgggatc aatggttctg gtatgcattg caatatgtct
       61 ttgttcaatg atgaaggaaa tgttttctat gataaagacg gtgaattgga actaagtgaa
      121 acagcttatc atttcttagg tggtttgctg aaacatgctc gtgcctacac agccgtttgc
      181 aatccaacag tcaattctta taaacgtctc gttccaggtt atgaagcgcc tgtttatgtc
      241 gcatggagtg gccgtaatcg ttcgccatta gtacgtgtac cagaatctcg cggtttatcc
      301 acacgtttgg aattgcggtc agttgatcca tctgcaaacc catacttggc aatggcagta
      361 ttgttgcaag caggactaga cggtatccgt aatcagatca caccgccagc agctgttgac
      421 cgtaacattt atgtaatgga tgaggaagaa agagaagctg cacatatcca ggacttacct
      481 tcaactatcc ataatgctat caaagaattg cgtaaggata atgtaatgat tgacgcatta
      541 ggacaacata tcttctcaaa ttttgttgaa gcaaaacgtc tcgaatgggc ggcattcaga
      601 caaactgttt ccgagtggga aagagaacaa tatctagaac tttattaaaa caggaatacc
      661 gtgaattaga ggctgtgaaa tcagcgtttt gacgagaaac taacggaaaa atagttcctc
      721 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttagtgga
      781 gaggttgtga aaaagctgcc ctgcaacaag taataagaat agccaagcaa aaacagcttt
      841 tcttgttttt gaattggctg aacttattac cgcagatgca agcttttgaa caccgtttac
      901 caagaggttt tgaaaaagac gtttagtttc gagtattaag aaaaattttg tgaaaatggc
      961 tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aataccgttt
     1021 atccagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacag
     1081 cttctcatgt ttgatggata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
     1141 tatttagaaa taaggaggca cgacttttgt cgcatctcct tattttttac ttatgtgatt
     1201 cgttaccttc aatgaatccc tacaaggcac ttttgttgaa aaaaaggtca tctttgaaaa
     1261 aattagtgga attgttttgt ttttgatatt atttaataga aaagatatgc caaaaatccg
     1321 agaactgtgg actacgtttt ttacgtaaag gaggtaaata agggtgaaag aaaaagattg
     1381 tttctacgat aagctatata aactgttgct gcaagtagga aaaagtagtt ctttgttagg
     1441 tgggatatta tctatcataa tgtttgtttt atttttttat ctattatttc acttaaaatt
     1501 tttcagtaac gattttctga tttgcttagg tttaacgtta gctatgttta tatcaagctt
     1561 attatgtggg ttaaattttt tttgcactca taaaggactt aagaaataat ttctcaaagg
     1621 atatcgaaaa agaaaaaatt gtaagcggat agtattcatt tgagtacttg actttccctt
     1681 agtcagcttg tattatacac cttgggcacc cttttagagg gtcagaagtt tacagaaatt
     1741 tcggctccct attcttattg aagaataggg agctttgttt attgtctgag acttttcctg
     1801 cacataaatt aaaaaggagt ttttgaactt atgacaaaat atatttttgt gaccggcggt
     1861 gtcgtttcat caatcggaaa agggattgtt gcagcttctt taggacgctt attgaaaaac
     1921 cgcggcttaa aagtaactat ccaaaaattt gatccataca tcaacgtgga tccggggacg
     1981 atgagcccat atcagcacgg agaagtattc gtaacagatg atggtgctga aacagatttg
     2041 gatctgggcc actacgaacg ttttattgac atcaatttga acaaatactc caacgtgacg
     2101 acaggaaaaa tctattctga agttttgcgt aaagaacgta agggcgaata cttgggagca
     2161 actgttcaag ttattcctca tattacaaat gagatcaaag aaaaaatcat gcgagcagct
     2221 aaaatgactg attcagatgt gatcattaca gaagtagggg gaacagtagg ggatatagaa
     2281 tctttgccat tccttgaagc acttcgtcaa atgaaagcag atgtcggtac agataatgtt
     2341 atgtatatcc atacaacatt gattccttac ttgaaagctg ctggagaaat gaaaactaaa
     2401 ccaacgcagc acagtgtaaa agagctacgt ggtttaggta tccaaccgaa tattttggtg
     2461 gtacgtacag agcaacctgt ttctcaaagc gtgaaaaaca aattggcaca attttgtgat
     2521 gtcgaaccag aagcagttat cgagtcgcca gatgtagata cactttactc catcccatta
     2581 gttttacaag cacaaggaat ggatcaaatt gtttgcgacc atttaaaact agatacacca
     2641 gaagcagaca tgacagaatg gaaagaatta gaagaacgtg tcttgaactt gaagaaaaaa
     2701 gtacggattg cgcttgtcgg taaatatgtc gagttaccag atgcatatat ctcagtagtt
     2761 gaagcattga aacattctgg tttcgcctat aattcagaca tcgagatcga ttggatcaaa
     2821 gcacaagaat taacgagaga aaatgtagaa gaacgtttga aagacgcaga tggaatcttg
     2881 gttcctggtg gatttggtga tcgcggagta gaagggaaaa tcgaagcgat tcgttatgct
     2941 cgcgaaaatg acgttccgtt tttaggtatt tgtcttggta tgcagatggc ttgtgtcgaa
     3001 tttgcacgta acgtcgttgg attagaagat gcgtcatcag ctgaaacgaa tccagataca
     3061 gcaaacaata ttattgattt aatggctgat caggaaaaca tcgagaattt gggtggaact
     3121 ttacgccttg gattataccc atgtaaaatc aaaaaaggaa cgaagacggc tgaagcatat
     3181 gatggagccg atgttgtgca agaacgtcac cgtcaccgct atgagttcaa taacaaatat
     3241 cgtcaattgt ttgaagaaca agggctagtc ttttcaggtg tctcaccaga taaccgttta
     3301 gtagaaatcg tagagctgcc agagaaaaaa ttctttgtgg gatgccaatt ccacccagaa
     3361 ttgatttctc gtccaaatcg cccgcaaaaa ttaatcaaag catttgtcgg agcatcatta
     3421 gcagatcacg aaacaaaata atttttgaac ttgatagaaa gagaaaagta gcaagagaag
     3481 gggataagca attttcttgt cacttttctc tttttctgat ttgttattat ttgtaggaaa
     3541 aaagatagaa agcaaaagac ttttttcgat taaagcgcta ttggatattt tattttttgc
     3601 aaaaaacttc ttgaaaattt tcattttcat tgattttagt agaaaactac cgaaatgttt
     3661 ggtaaaatag ggtcgttggt taagaattgt cttaacaaat ttagttttac aaaacttagg
     3721 aggaatttta ttatgccagt agtatcagga gctgaatttt taaaagctgc tcgtaaaggc
     3781 ggttacgctg tcggcggatt caacacaaac aacttggaat ggactcaagc aatccttgaa
     3841 gcggctgaag caaaacaagc accagtactt atccaaacat caatgggtgc agctaaatac
     3901 atgggtggtt acaaaatctg taaagattta atcactgatt tagttgaagc aatgaacatc
     3961 actgttccag ttgctatcca cttagaccat ggtgattacg aagctgcttt ggaatgtatc
     4021 gaattaggct atacttcagt aatgttcgat ggttcacatc taccatttga agaaaacttg
     4081 aaattagcta aagacgttgt tgaaaaagca catgcaaaag gtatctcagt tgaatgtgaa
     4141 gttggttcaa tcggcggaga agaagacgga atcatcggta acggcgaatt agctgatatt
     4201 gaagaatgca aacaaatggt tgctacaggt atcgactact tagcttgcgg tatcggtaac
     4261 atccatggtc aatatccaga aaactggaaa ggattagctt ttgaccactt acaagcaatt
     4321 gctgatgctg taggtccaga catgccttta gtattacatg gcggatcagg tattcctcaa
     4381 gatcaaatcg aaaaagctat ctcaatgggt atctcaaaag taaatgttaa cactgaattc
     4441 caattatcat ttgctgcagc tactcgtaaa tatatcgaag ctggtaaaga taaagaaggt
     4501 aaaggattcg accctcgtaa attattagct ccaggtaaag cagcaatcgt tgaagatgct
     4561 aaatctcata tcgactggtt cggttcagct aacaaagcgt aagcatccac ataacagata
     4621 cgatatatag gaatggagaa cgacttttga agtcgctctc cattcttttt tacttaaaac
     4681 ggcagaccat gctcgtttct tttttttatc tatgataaaa tacaagcata tccatgaaaa
     4741 aagattaata aacaaggtgg aaacagatga aaaaaatcgt aatcaaagga aaccgacctt
     4801 tagctggtga agtaacgatc agcggtgcaa aaaatagtgc agtcgcattg attccagctg
     4861 caatcttagc tgattcccca gtgatcttgg aaggtgtgcc ggacatacaa gatgttcatt
     4921 cattaattga aattttagag atcatgggcg caactgtcca tttttccaac aacatactag
     4981 aaatcgatcc tcgagaagtc gtatcgattc cgatgccgca cggaaaaatc aatagtttgc
     5041 gtgcttctta ttattttatg ggaacactgc ttggtaaatt tggcgaagcg gtagtaggcc
     5101 tgccaggtgg ctgttattta ggccctagac cgattgacct acacgtcaaa ggatttgaag
     5161 ctctaggtgc tgaagtgaca aatgaacatg gtgccatgta tttacggaca gaaaataaag
     5221 gattgcgtgg caatcggatt ttcatggacg tggtttcagt cggagcaacg atcaatgtga
     5281 tgttagctgc tgtaaaagca aaaggacaga cagtaattga aaacgctgca cgagaaccgg
     5341 aaatcataga tgtggcaact ttactgaata atatgggggc aaaagttcga ggagcgggta
     5401 cagatgtgat ccgtatcgaa ggagtcgaaa cattacatgg ctgtcggcat ttcatgattc
     5461 ctgaccgcat cgaagcagga acctatctag cattagcagc tgcagtagga aatggtatca
     5521 aagtgaaaaa tgtgatcttt gaacatttgg aaagctttat agcaaaattg caggaaattg
     5581 gtgtccatat gaaaatcagc gaagatgaaa tcgaagttta cccctcgaaa gaattaaaac
     5641 ccgcaaacat catgacctat ccttatccag gatttgccac agatctgcag cagccactta
     5701 ctgcgttact attgatgact acaggcactt cagaaatcat cgatacgatc tatgcgaaac
     5761 gtgtgaacca tgtgccggaa ctagcacgaa tgggcgctga tattaccgtg gaaggaaata
     5821 tgatcatcgt gaatggtcca aataaacttc acggcacaga agtcgtggct tctgatcttc
     5881 gggcaggtgc ttgtttagtg attgctggac tattggcaga agggacaacg acgatttaca
     5941 atgtagatta tatcttacgc ggttatgatc atattatcga gaaactaaca gccttagggg
     6001 catcaattga aatgatagag gaagtgggag ctgaatgagc gattatttga caatggcaga
     6061 actagagaac aaaacgttaa aagaagttta tagttatgcc aaagaattta aaattcctta
     6121 ttacagtaag atgaataaaa aggaattatc tctagctgtg attcgtgcac aagcagagaa
     6181 acaaggattt tattatatgg aaggaatatt ggatatcgtt ggtcaagatg gttacggttt
     6241 tttacggcca atcaattatg gaccaagtgc agaagatatc tatatttctg cctcacaaat
     6301 cagacgattc agtttgagaa atggggacaa agtggcagga aaagcccgtc cacctaaaga
     6361 atcggaacgt tattatggtt tgatgcacgt ggaaagcgtt aatggaaaag atccggaaga
     6421 agcaaaagaa cgtccgcatt ttccagcctt aacacctctt tatccgcaaa gacagcttca
     6481 actggaaaca acaccgcaaa gactatctac ccgaatgatc gatatctttt ctccaatcgg
     6541 ttttggtcag cgtggattga tcgtcgcacc gccaaaagca gggaaaactt ctgtattaaa
     6601 agaaatcgca aatggtatca ccgaaaacta tccggaagta gaattaatcg ttctactgat
     6661 tgatgaacga ccggaagaag taacggatct agaacgaagc gtgaaaggcg acgtggtatc
     6721 ttctacattt gatctgcagc cgcaaaacca tactcgagtt tctgaattag tattggaacg
     6781 cgcaatgcgt ttagtcgaag ataaacgaga tgtcgttatc ttgatggata gtattactag
     6841 acttgctcgg gcctataact tagtcgttcc accaagcgga cgtacattga gtggcgggat
     6901 tgacccagcc gcattgtata aacctaagaa atttttcggg gcagcaagaa acatcgaaga
     6961 aggtggcagt ttgacgattt tagcgaccgc tttagtcgat acaggaagcc gtatggatga
     7021 cgtgatctat gaagaattca aaggaactgg aaatctagaa ttacaacttt cacgtgaatt
     7081 atctgaacgt cggatcttcc cagccatcga tatcaaaaaa tcaggtaccc gaaaagaaga
     7141 attattgatg ccaagtgaag aattagaaga aatttggaaa cttcgaaaaa atatgactgg
     7201 agattcatta gaatatacgg aacaactgat ccgctttttg cgtaaaacaa aaaacaatca
     7261 gcaatttttc aaagaatttc aagatgtctc ctttggtaaa aaaaatccga ccagaaatct
     7321 gaaaagataa ttgcaacctt gactgaaaca tgttaagatg ttaatgttgt aaatcgacaa
     7381 atcaactctg gttcagcaaa tggagcaggg caaaggagcg aaaaaatcat gaaagaaaac
     7441 atccatccag attaccaccc agtagtattc atggattcaa caactggttt caaattcttg
     7501 tcaggttcaa caaaaacttc acaagaaaca gttgaatggg aagacggtaa tacataccca
     7561 gtcatccgtg tcgaagtaac ttctgactca catccattct acactggacg tcaaaaattc
     7621 acacaagcag acggacgtgt ggaccgtttc aacaaaaaat acggtctcaa agacgcaaac
     7681 gctgcggaat aataaaaacg ctgataactc aacgtttagc agactatctg atagggatag
     7741 tctgtttttt tatgtttttt gccaatttaa aacttcgttt gatgctgaaa aagtcgtagg
     7801 atcataatgc acaaaacaat gaaaaactag tatatagttt taattatcca cttgataaaa
     7861 acaccttata ttaacgaaat gaagaggggt tatttcgaag taaaaaacca tttacctaaa
     7921 attagaagct aagctttcct ttcaaggaag gctttttgtt ttgttataga cttgcgattg
     7981 tttgtgagct agatcgttca ttttaatgac ttatacacgc ggatgatggg tctttttcct
     8041 ctgttcattc ataatattga acggtacagc tagcgatgct atactgacct cagttccaag
     8101 aaaacgttaa cattttttgt gggggagagg ttttttatga ggtttgatca attgatcgca
     8161 gaagaactga ttgttttcga tccatcgata gattcaaaag gatcattatt tgagcgaata
     8221 ggggatgtgt tgaaaaaaac aaataaggta agaaatgttc aaaaagtcgt taaggcacta
     8281 ttcaaacgag aggaggagat ctccacagga atagaagatg gatttgggat tccccatgca
     8341 aaaagcaaat atgtgacaaa accagcactg tgtttttttc atacagccaa attaacggac
     8401 tatagcggga tagatgaaat gccaattgaa tatatttttg ccatcgtagt tcctgaaaaa
     8461 tcagcagata tgcatctaga gattctcagc acactctcca gacgattgat gaatgcagat
     8521 tttagaaagg gaataaaaga agccactacg ccagcagata tcatcagaca gattagtcaa
     8581 gaatgaaaat agtagagtct ttgtccgagt atcgatagtg aagaaaaagg aggaaaataa
     8641 tcatgaaaat cgttggtgta actgcttgcc cgactggaat tgctcatact tatatgtcgg
     8701 cagaaaaatt agaagtaaca gcaagaaatc taaaccatca agcaaaattc gaaacacaag
     8761 gtgtaaaaac agaaaatcag ttatctgaac aagaaatcaa agaggcagat gcaatcattc
     8821 ttgcagtaga taaagaaatc gaattggacc gatttgccgg gaaaaaagta aagcgtgtct
     8881 ctacaagccg agcaatcaaa gaaccgcaag tagtaataga cgaagcattg agagatattg
     8941 gcgtattcgt tgtgtctaat gaaaaagaac cagctgcaaa tgagaaaaag ccgacaatct
     9001 ataatcattt catgaatggt gtgaattaca tgcttccatt tgtgatcgca ggcggaatca
     9061 tcatcgcact gagttttgcc tttgggatta cagcggctga tcccaactca gcagactata
     9121 atgtgttagc agctgcattt tcaaggatcg gaggagatac agcgtttgcc atgatggttc
     9181 ctgcgttagg cgcggggatt gctacatcga ttgctggaaa agcaggtttt gccccaggaa
     9241 ttgttgccgg attactggcc tctacaggtg gttcaggttt tcttggcgga atgattggtg
     9301 gcgtgctagc agggtatatc tgtgattttc tggcaaataa aattagcgta aaaaaagaat
     9361 tttctgctat ttatcagctg attgtcgtcc cctttttatc gatcttaatt attgggcttt
     9421 tgatggtatt tgtgattgaa caaccgatca catgggtgtt ggatgctttg acaaattggc
     9481 tgaatagctt gggaaataca tccgggctat tgttcggatt acttatcggg atcatgatgg
     9541 cagctgatat gggtgggccg atcaataaat cgacttcgac tttctcgatc ggtttgatgt
     9601 cagctggagt aaatgcaccg attgctgctt gtatggctgc aggaatgaca cctcccctag
     9661 gtattgcttt agccacttta ttattcaaac ataaatttac caaagaagaa cggacttcag
     9721 gtcaatcatg ctggatttta ggcgcttcgt atattacaga aggtgctata ccgtttgcgg
     9781 tatctgaccc gatccgcgtg atacctagtt tgatgacagg ttcagcagtc gctgcaggga
     9841 tttccatggc agtaggagtt acttcaatgg cacctcatgg agggatttgg gtgatgctaa
     9901 ttccaaatgt tatgaacaat ttacctatgt atgtcttggc aattgcatca ggtacagtca
     9961 ctacagcctt tatgcttgga ctattgaaaa aaacagtgaa agagaaaaaa caagacaagt
    10021 ggacgaaaaa tgaaaatcta acaaatgagg tggaagcgta atgttatata caatgaaaga
    10081 gttattgaaa gtggctaaag agaatcaatt tgcagtacca gccttcaata tttgcagcta
    10141 tgatatgatg aaagcaatca tcgaagaagc agagagatta gaagcaccag taatcttaga
    10201 aattcatcct gatgaaatcg cctatttaag tgatgagttt gttgcggcag taagagccta
    10261 tgcttataca agtaaactgc cgatcgttat ccacttggat catggcggta cgatagaaga
    10321 tgtcttacgg gcgattcgaa acggctattc ttccgtgatg atcgatgcgt ctttgaaatc
    10381 attcgacgac aatgtacaga ttacgaaaaa agtggttgcg ttagctcatg cggtaaatgt
    10441 ctcagtggag gctgagcttg gaacgattgg gaataatggt tctgctgaag gaggtgctga
    10501 aacaatcatt tatacagatc ctgatcaagc gaagattttt gtgaaagaaa caggaatcga
    10561 tacacttgct gtagcaattg gaacagcgca tggcttgtat ccgaaaaaca gaactccaaa
    10621 attaaatatc gacttactga aagaattgaa ccaacaaatc gatcttccat ttgttttaca
    10681 tggcggttca ggtaatccgg acgcagaagt cagcgaagca gtaaaatacg gagttgccaa
    10741 agtgaatttg agctcagatc ttaaaagcgt ctttttcaat gctttgagag aaatattgag
    10801 tgaaaatcca tcgatgtacg agccgaatgc aatttatcca gcagctaata aaaaggtaca
    10861 agaagtggtc aaacataaaa tggaaatttt gaacacagta ggcaaggcta gtttatacta
    10921 aaaataaaaa tgtgtggtca agagggtatg acaatagtct tgtcataccc tcttaaccac
    10981 acacgatgtt cagaagtagt tttttcgaaa aatttcttct tatttctcga agccaatcac
    11041 ttctgtcaca acctcttttt tcctgttact atataaagta aatggggtga ttttatgttg
    11101 aatgaagtgg agaatagaca gcaggagatt gtgaattatc taaaaaacaa ccaatttgca
    11161 agagtcaccg atttgatcga gttagtaaat tatagcgaag ccacgattaa acgtgacctt
    11221 gtttttctgg aaaaaagtgg attgatccgt agagttcgcg gtggcgccat gcttattgac
    11281 aatcgaaaaa ttgacgtgcc ttacctgatg aagatcacca aattagaaga agaaacagaa
    11341 aaacgtaaga ttgcagatac agcttcttct ttgattcgag atgatatggt cttattcttg
    11401 gattccagta caacctcttt acacttagtc agaaacctag caagatttga tggtttgcag
    11461 atcattacga atggcatcgt cacagctgct atgttgagtg aatatacaac ggcaaaagta
    11521 tctgtattgg gaggggcaat tgttccgaaa cgagcaacaa tcaacggttc aaaagcctat
    11581 aacgatgcat tgtcatatta tgcggattta gcgataatga gttgtcgcgg gctagatttc
    11641 agccaaggag taacagaaac tcatgaagga gaggcaatgg tcaaaaaagc ttttagaaaa
    11701 caatcagacc ggcttgccgt tttagttaca cagcaaaaaa ttgcacataa attcatgcat
    11761 caagccattg cttttcatga aatcgattat ctggtgacaa gtaaagaact gacaaaagaa
    11821 cagtatgcga aagtaaagga acaccatatc gaatgtctgt acggataaat tattacgtga
    11881 atgttctaag tggcttcctc atttttagat aggaccttat ctttgctcgc ttctctcagc
    11941 cgatggtata ataatgaaaa ggttatcttg ttagataatc ttactttttt atccaggaga
    12001 aaggagtgca gaaaatgaaa catgtgaagg cgttggtagt gaaggcaatc atgatctggg
    12061 cgattctgtg ggtcgtgctg acaggtttat acggtgttag ctttatggat tcaacgattg
    12121 ttggggtaat tattgtagtc atgatctatg tgctagggga tctgttgatc ttacgtaaag
    12181 tagggaacat cgcagcgacg atcgcagatg ctggttcagc tgcagttatc ttgtggctct
    12241 atctatattt tatgaatgat acagtcgata tttggatgaa agtattgatt cctgctctat
    12301 tgatcggtgt ggcagaatgg ttcttccaca agtggttgct aagtcaaggc atcgtaccag
    12361 atgaacggaa aatggaataa atcaacagtt aaactattct tatcgataaa tgaaagcttt
    12421 aaaaataaat agatgtttaa ttaagaataa tcataaacta tttttactca aaaaaccgct
    12481 cagattattt tcagatctga acggtttttt ttatctattc atcaagatca aatttcattt
    12541 ttcaatgatt caatcaattc gccctctgct tcatgactat gttttcggcc attaacaaga
    12601 tagagtgctg tacatatcaa tggaatgact ccggcaatca catacaatcc tctaaatgaa
    12661 gtaagagatt ttagcatccc catcaaataa ggaccaacgc ctaagcctag gtctaaacca
    12721 ataaagtagg tagataaagc aatactgatt cgatgttcgc ggacgatttt taaacaaaca
    12781 gcctgtccat ttgacataaa cgtaccataa cctaggccga ctaatgctcc agagataagc
    12841 aatgtcacac tattcgtcgt tacactcagt aaaaataatc ctgctgttaa gaataaatag
    12901 ctaggataca tcacgtattg ttctcctttg acatcgaaaa tccttccagt caatggacgt
    12961 gtaaatgtga tgaccaaagc atatacgacg aagaaaaatg acccagcagc aactaagtgg
    13021 attacttttg tataagaaga taaaaatgat aaaacacttg aataagaaag acccatcaaa
    13081 aaggcaattc ctgtaatgaa aagtgctttc ttttcaataa aactattgat attccatgag
    13141 cgaagagata ttttttgttc ttcagataat ggaatatttt ttacaggaaa gacaaggcag
    13201 gcaatcgtaa caaagaagac cagtacaatt gaaaaccaga taatgaaacg aaaatcagtc
    13261 atattcagca aaatcattcc taaaaatggc ccaacagcgg cagctaaact agtacttaat
    13321 ccataataat tgatgccttc ccctttttta gatgcaggga tatatgcagt aacgatagca
    13381 tttgtagctg tagaaactgt accgtagccg aatccattca agaatcgaac taaatacaat
    13441 gccccgatag ttggcgtata aagatatgct aatgttgaaa ggagatagaa gatcgcgccg
    13501 cctcttaagg taaacttccg accaaatagt tctaactgtt tccccatgaa taagcgtgcc
    13561 agcaatgttc cgatgatata gatcccagaa gcaaaacctg cttggctcaa gctggcgtgt
    13621 aaggtgtctt gcgcaatgac tgccatgatg accatcaaca gataataaat caggtagaca
    13681 ataaaattga tcaatgtgat tccaataaaa tatttattaa ataatttctt ttccatgtat
    13741 gttatacatt ccccttaagc tgaatatgct tttaattttt gtatttgaac ttctacatcg
    13801 tgataattgg atggatagcg atcaacaagt tggcgaataa ataaatctct tgtagacata
    13861 aacagattta aaaattcttc gcattcgttt gttggattct catccactgc tttgatcaat
    13921 tcgatcgagt tttgatacgc atcatttaaa aagactaaag aagctttcat atcaaatctt
    13981 gtttgatatg ctgtttctaa tcgattaatg attgtgttca tccagtaatt catgtttttt
    14041 ccctctattc ttaaaaaatt ttacttctgc agtataacga ataaaaaaaa ggaaaaaaat
    14101 cgcataagtg atggaaaaag gtacttaaca aatgttaata tcattagtaa aatacctttg
    14161 gactttaaaa agttttaaag tactgtatga tctttttaga tgaaagtttg gatgggggag
    14221 catcattgga tagtagagtc ttaaaggaat atttggataa aaatgatttt ccgattatac
    14281 gcaaaggata tcataaatat ttgacattcg aagggatgga agattcccat acgtatatct
    14341 tgaaagaggg aacggtaaaa gcaagtgtta tttcaaatga tgggagagaa tttaaccttc
    14401 gctatatcaa agatcttgaa atcgtctctc ttttaaaaga cgaatattca caattcatcg
    14461 attcgccgta taatattcga gttgaatcag aatatgcaga gttttatcga atcaatcgtg
    14521 tgcgcttttg gcaaaatatc aaccaagacc caaagctaca acactatgta aaagagtatt
    14581 accgctatcg tctgctttat tcgatgaaaa aaatgcagca aatgctatta aacggaaagc
    14641 tgggagctgt ttgtacgcaa ctttacgaat tatatgattt attcggtgtg cggacagaag
    14701 aagggatgct gatcgatttt tcgatcacga atgaagaaat cgccaaattt tgtggcatct
    14761 caactgctag cagtgtaaac agaattttaa aacaactgaa aaaagatggt attatcaccg
    14821 ttaaagacca tcatatcgtt attttgaata tggacttgtt agaagacaat attatctttt
    14881 gatgaacaaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaacggaa
    14941 aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca
    15001 ccgtttacca agaggttgtg agcctgccgt ttaactctga gtcacaagga acaatttcaa
    15061 aaaacagctt ttcatgtttg atgaatattg tgacttgtga ccgaggagtt ggtaggagaa
    15121 caccgtttac caagaggttg tgaaaaagct gttctgcatc aagtaataag aagagccaag
    15181 taaaaacagt ctcttatgtt tttaacttgg ctggacttat caccgtagat gtaagctttt
    15241 gaacgccgtt tatttgcaac aaaaggggct gtgacacgat ttttgtcaca gccctttttt
    15301 tgcgaacatt catttagcaa tttttagaaa cttctgccgc caccgccaaa agtcccgcca
    15361 ccgctagaat gggtagtact tccgccaccg ccgcttccag gaggtgggct tttggggatt
    15421 cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt tttcagttaa tttgatactg
    15481 gatttttctc ggaatggata tttgtagatg cctgatttca attgataacg cgaaacagtc
    15541 acaaaataaa agacaagact aaggataaga gccaaaacaa cagcaatgat gatttctact
    15601 gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt cgtctacacg ataatgtcct
    15661 cctggaactc cgtcttccac atatgttgct gatttagaca ggtaagcttt tgcagcattg
    15721 taataggcag catttgtcat ctgatcataa acatcatcta agatcgaatc gattctgcta
    15781 tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt agatttcacg ctgtcccata
    15841 tctaatagca atactgagcc attttgatca tttccgactg cgtctcttaa gtaattatca
    15901 gcaaagtctc ttggttcttc tgaattagat gtagtcgtta cgatcaagac tcgtcctttg
    15961 attttttcat tcagcttttc tgcctgttgt tccaaagatt ggatttgatc tgttgtgaat
    16021 aacccagcct catcatttac cgtagctgta tctgcatgag tagccaccgg ccagccaaca
    16081 cttactagca aaagaaagaa taaaaaaaat tttttcatca tatcaagtaa cctccgatca
    16141 gaaagaggat cgctagccca gcgaaaatcc caagagcggt cagtcccaat tttttctggc
    16201 tgatcggcag gacaccgctg acctttcctg tttgtccatt cattgcgtag tagtagactt
    16261 ttttacttga gtcattgctt cgataagtaa caagccatac cggcagtaat acgtaattat
    16321 ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt caatgttgta tatccatttg
    16381 cggtatcttt taacagcttc tctgaatagt cttttaattc agattgaatc tgtgatttga
    16441 ttgcctcata ttcgatatct cttttttctg cttggaaccc ggctaaatac tggcttttaa
    16501 aatcaattgc tttatctaac gggaaaggtt gcacgccttc gaccatcttt tgttgcgtat
    16561 tttttgataa ggcatttttt actagttctt taaaagaaag cttgcctgct cttgaaacag
    16621 caaattgctt cgtttctgtg tattcgatgt cacctatccg ccatatgcgg ataactgtac
    16681 cattcgcctg catggcacca tctacttcag catctgtagc ccaataaggg aaatacacac
    16741 ctgtcaactt atcgatttga tccttattga aaaaagcttt gggaacaaac cattttttct
    16801 tggtccaagc gagaaatctg ttgattgctt cttctttctc gacagcaaaa ggaagtactt
    16861 tatttggcaa gaattctccg cttaaacgtc ccgatagtac gacgggatta tggcagtagt
    16921 aacaataagt cgcagcagtt gtcgcatcag tcacgatctc tgcaccacag ttgggacaaa
    16981 gaaaaagatc tatcgctgcg gtattatctg cagattcgtc cgctgtatct gaaaaaccgc
    17041 ctgcctcgtc aaccgctttg cgctcggctt cattcatatc ggcaagctgt tcttgggctg
    17101 taaaagtaaa atcttcggta ggtgaaggtt cttctttgct ttcttcgcca gccatacgcg
    17161 cttctttttg cttttgctca tattggctga cttcttcttc tgtatagata ttcaggcagt
    17221 attcgcaatg aaatttttgg tctttgggat caaaagtcaa cggaccgccg caattaggac
    17281 atttatgtgt aagaacatcc atcagctgca cacctttcta aataaattaa ttaacgggga
    17341 ttcctttaaa cggtttacca cagtttggac agaatttagg gatgccgttg gaaagatcca
    17401 cgatctcatt gcattcactg catcgcattt gtagcttagg ctgaacgttt tctacaggtt
    17461 tggcagctcc gcagttggaa cagaattttc cggaattttc tgtcccgcaa actggacatg
    17521 tccaagtatc cgcatttcct tgtgcagttt gttgattttg tttttctgct tgttgtttga
    17581 tttgttcttg gttattttga gctgcttgag aaagataaga accatttgca ttcattccca
    17641 tacctacacc cataaagccg gtcattgctc ctgcttcatt ttttccggct gcttccatac
    17701 cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct aggatcacct agcatcgccc
    17761 cttcgttacg catattgatc agtttcacag aatcatcggt gtaagaaatg ctggctacag
    17821 ccacagaaac aatctccatc ccacgtaatt cacgccaaga atcatccaat gcagtatcca
    17881 tgtatttgct taattccaaa ctcttagatg gtacataaga aatccgttga ccatctgcag
    17941 acatctgatt gattgctgat tgtagcgctg ttaggaattc agctaaatac tgctcattga
    18001 tatcattgat ttccacttgt gttttgtttt tagggattgc gtttgtataa aataaaatcg
    18061 ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa gaataattct gcgttataga
    18121 aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt gattcccttt atttcttgga
    18181 gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc gccaaatttg aatcggtcaa
    18241 aagtttcagc aattgcttct ttcagtgttc cattgaacat ggaaggagct gcgtcatttt
    18301 taatggtgta ataaccttcc tcagcggtat aatcgatgat ttttccacca tcgaccaaaa
    18361 gcatcatcat gtttggatag acatggacga ctgttccgtc tgttaaaaca tcttctgttc
    18421 ctttacgatt ggagccacgt ttgtcatctt tgcggacttt gacacctttt gtcatgaccg
    18481 ttgtatcact catattgtct ggttcgatga cttctagcca ttgatctgcc agaccgccgc
    18541 caaccatact tgttgctgct ttgataagac ccataaaaaa ttcctcctct gattcagcta
    18601 gtgccaaaca attattgttt atttattata ccataggaag atgtgaaact tctcggacta
    18661 gagacggaat tgaaatggaa aattgcgtat tgtttaaata cctgaaaaag ccgagaagga
    18721 tagtagtttt tttacctaat atcacgtata ataagagata gtgaattttt taaaaaaggg
    18781 ggatccttat ggcatatcaa gcactttatc gagtctggcg ttcgcagcgt ttcgaagaca
    18841 tcgttggaca aaaagctgtg acacagacac taaaaaatgc tattgtttcc cacaaaacct
    18901 cacatgccta cttgtttaca ggacccagag gaaccggaaa gacgagtgca gcaaagattt
    18961 tcgcaaaagc aatcaactgt ccgaacagta aagacggtga gccatgcaac gaatgcgaaa
    19021 tgtgtcgatc aattactgct ggtacgcaag aagatgtgat cgaaatcgat gctgccagca
    19081 ataacggagt cgaagaaatc cgatttatcc gtgatcgggc aaattatgcg cctacaaagg
    19141 cagcgtacaa agtgtacatt attgatgagg tacatatgct ttcaacagga gctttcaatg
    19201 ccttgttgaa aacattggaa gagccaaaag aaagtgtcat attcatctta gcaacgaccg
    19261 aacctcacaa aataccagct acgatcattt cacggactca gcgatttgat tttaaacgga
    19321 tcaatacggc agatattgtc gaacatcttg aatttatctt gaaagaatcg actatcagct
    19381 atgaaccagc tgcattgaat gtgattgccc gttctgcaga aggcgggatg cgggatgcat
    19441 tgagtattct tgatcaggca atctcgttta gtgaaggaac aattacttta gatgatgcca
    19501 tgcaagtgac aggtagctta actgacgaaa tgatggacag ttacttgagt gcttgcgtga
    19561 aaaaagaagt accagaagca ttggaaacat tgaaccaaat tctcgcagcc ggcaaagaat
    19621 cacgtcgctt tctagaggat atgttacaat actgccgtga cttgctgatg tatcagcaag
    19681 cacctaacct agttgaggaa caatcgagta cgatcagaag tacatttaca gaattggcac
    19741 aaactgtttc agcggatcag ctgttttcaa tgataaagat tttgagcgat actcaaaatg
    19801 aaattcgttt taccaatagt gccgctattt atttggaagt cgctacagtc aaacttgcac
    19861 agctaccgtc tgcttctgtt ccttcagcag atgtagcatt tgcagatgat cagatagtaa
    19921 aacagctaca acaagagatt aaggaactaa gaaacgaaat caaggaacta cgcagtaata
    19981 caggaattga gaatacaaaa gtacagccgg tacagcaaac aaatcataaa aaacagacaa
    20041 gtacctatcg tgtaccgacg gaacgagtat ataaagttct gcgggaagcg acaagacagc
    20101 atctgaacca agtaaaagaa gtatgggaag acttgctgat gagtctatct gtcactcagc
    20161 gagcaatgct taaagcaagc gaaccagttg cagctggacc tacaggacta gtgattgcgt
    20221 ttgattatga gattgtttgt caaagagcag caaatgacga agaattgcag ctttcggtac
    20281 acaacaactt aagccgaatg atcaaagatt atgcgcctga tcctgtttat attacaagag
    20341 aaagctggcc agaacttcga agaaattatt tgacgcaggc acaagaagag ggaacagaaa
    20401 actcttttgc ttctgaagac tcagaaggag aaatcgagtt gcttcctcaa gaacaagaag
    20461 taccagaaga aacacttgta gtaaaagcag aggaactgtt tggctccgaa gctgttacag
    20521 tggtagatga ttaataaaaa ggagtagata attatgatgc gtggaatggg caatatgcaa
    20581 ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg cagaagcaca agagctgcta
    20641 aataaaacag agtttacagg taaagccacc aatgaattgg tcaaagcggt ttttacaggc
    20701 gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg ttgatcctga tgatatcgag
    20761 atgcttcagg atcttgtcgt aatggcagtc aatgatgcga tcgcacaaat tgatgtggaa
    20821 actgagaaaa aattagggaa atacgcaaaa ggtttgccag gattttagtt ttaaactgaa
    20881 ggagtaatga atatgcaata tccagaacca attgcgaaac tgatcgacag ctacatgaaa
    20941 cttccaggaa tcggtcagaa aacagcgaca agactcgcct tttatacgat cgatatgaaa
    21001 gatgaagtag taaatgaatt tgctaaatct ttgttaagtg taaaacgcga tttgcatttt
    21061 tgtagtatct gcggaaacat cacagaagaa gatccctgcg aaatatgtag aaatccttca
    21121 cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag atgtaatggc aatggaaaaa
    21181 atgcgagaat atcacggact ttatcatgtt ctccatggtg tgttatcacc gatggaaggg
    21241 acaggtccag aagacatcaa tatttcttct ctgttgcagc ggctgcatga tgcagatgta
    21301 aaagaagtga ttatcgcaac aaatgcaaca acagagggag aagcaacagc tatgtatctc
    21361 tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc tagcacatgg tctgtctgta
    21421 ggctctgata tcgagtatgc agatgaagtt actttattga aagctgtcga aggaagacgt
    21481 gagttataaa tttctaatta agtgtttttt cagatacaat ataaaagcag cacaagagat
    21541 gggggcaaca atgtgaacgg actatttatt acaatcgagg ggcctgatgg tgcaggaaaa
    21601 accagcatct tgaatgaact ttaccctctt ttgaaagaag tagcaaaaac agaaatcatt
    21661 caaacaagag aaccaggagg cattccgatt gctgaagaga tccgagcagt gatccttgat
    21721 ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc tttatgcagc agcaagaaga
    21781 caacatctag tcgaaaaggt tctgcctgct ctggcaaaag ggaaaatcgt tttatgcgat
    21841 agatttgtcg acagttctct tgcttatcaa ggtgcaggcc gacgcatcgg cgttaccgag
    21901 attgctcggt tgaatgagtt tgctacagaa ggtactacgc cggattttac cctttatctt
    21961 gacgtagatt cagacaccgg attaagaaga atcaagaaaa acagacaaaa tcaaatcgac
    22021 cgtttagatt cggaaggttt ggaattccac cagcgtgtac gtcatgctta cttaaagctg
    22081 gcagaagaga atccggaaag aatccacaaa gttgatgcaa gaaaaagctt tgaagaagtg
    22141 ctgcaaacaa gttatcatac aattattgaa cagtatccgc aattttttga aaactaggaa
    22201 ggtgaacgtt tatgaaaatt attttagcga ttatccaaga caaagacagc aaccgtttat
    22261 caaatgaatt gattgatgca aatatccgtg ccacaaaact atcatcgaca ggcggcttcc
    22321 ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga tgaccgtgta gaagaagcat
    22381 tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt tgtatctaca cctgttacac
    22441 tggatatttc gatggatggg ggcgtccctt atccagtaga agtagaagta ggcggtgcca
    22501 cagtcttcgt cttacctgtt gaaggattcc atcaatttta ggaggagctg agctatgaat
    22561 caagaaataa acttggctca aatgcaaccg atcgtgtatc agcagcttca acggagtttt
    22621 gagcatgggc gtatcgctca tgcttatctt tttgaagggg agaaaggaac aggaaaacat
    22681 gaagtaggta tatggcttgc acagcacctg ttctgtaccc aaatgaaaga caatctgcct
    22741 tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag aacatccaga tgttttggtt
    22801 attgaaccgg aaggacaaac catcaaagtc gatcagatca gacgtttgca gacggaattc
    22861 agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga tcaaagaagc tgaaaaaatg
    22921 aatagcagtg cggcaaacag tttgttgaaa ttcttggaag agccccctgg agatttttta
    22981 gcggtgttag aaacagatgc agtaggaaga attctcccta ctattcaatc tcgttgtcaa
    23041 gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc atcgattgca agaagagcag
    23101 attccattgg aaaaagcaaa acttttggct ttcttaacaa acagtttagg aaaagcagtt
    23161 gaaatctctc aagatgaatg gtttaatgat gctaaggact tgatttatca atggttcgtt
    23221 tacttgcaaa agcaagatac tcaagccttt atctatgtcc aaaaaaaatt ggtaaaatca
    23281 tttaaagaaa aaacacaaca gtttacagga ttatctgtct tgatgtttta ttatcaggaa
    23341 gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag tgaaacagat aaaccagata
    23401 attgaacgga tattgatcgc agagcagaaa ctacgcagca atgtcagttt tcaggctgtt
    23461 gcggaacagt tcgttttgca tacgattttt tagcttgccg caaaggatga tgatcaagga
    23521 ggatggaaat ggtagaagta gtaggtgtcc gatatagaga tgcaggtcat atctattatt
    23581 ttgcaccagg agactctgaa tatgtttata gtgacaaggt attagtcgaa tctcagcaat
    23641 ccttacatat tgctactgtt gtccttccta aaaaagagat tgatcccaat gaccttcctg
    23701 aagctatccg tccgatcttg cataaagcgt cacaagctga attagaaaaa gacaaaaaaa
    23761 acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga aaaagtgcag ttgcgtgagt
    23821 tggaaatgaa aatggtcaaa gccgaataca catttgatcg aaataaattg attttttctt
    23881 tcacagcgga cgggcgaatt gactttagag aattggtaaa agatctggcc gctgtatttc
    23941 gtactcgtat tgagcttcga caaattggtg tccgcgatga agcaaagata ataggcggaa
    24001 ttggtccatg tgggcgtcca ttatgctgtt cttctttttt aggcgatttc gtgcctgtat
    24061 caatcaaaat ggcaaaagat caaagtttat ctttgaatcc aacaaaaatt tccggtttat
    24121 gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga atacgagaca gcgaaaaaag
    24181 aactccctga ctttggaaaa gtaattgcta cacctgatgg taaagggaaa gtcgtagggt
    24241 tgaatctgct tagccgtatc gttagtattc ggctgactgg acgggaaaat cctgtcgaat
    24301 atgaatggga tgaattgaaa gaatttatcc aagtaggtga gatgaatgga taagagatca
    24361 ttatacgacg gattgaatca attagaatca gagttgagaa atacattaac agaacttgtt
    24421 gaaatgaagc atgctttgca tgagatcgta gaaaaaaata caactttgga gatggaaaat
    24481 caacgtctgc gggaacacct gcgggaattg aaccaagttg cacaagctcc agctgaaaat
    24541 gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa aaatttatga agaaggattc
    24601 catgtctgct acgatttata tggatcaaga agggacaatg acgagccttg tgcgttttgt
    24661 ctagaagtga tctatcgcga aagcggtcga tgaccaataa caagaggaga ctggagcgga
    24721 gaaatactgc tccagtcttt ttattaagga gattgtgaca gatgtataat caaaaaagtt
    24781 tcaagggaaa tccggtatat ggggatttat atcttgtacc gacgccaatt ggaaatttag
    24841 aagatatgag ctacagaagt gttcgtatgc tgcaagaatc cgatcttatc gcaagtgagg
    24901 atacgcgtaa tacacaaaag ttattgaatc attttgaaat ccatacaccc caaaaaagtt
    24961 tacatgaaca taattataaa gaacgtgtcc ctcaattgat tgctttgtta aaagaaggaa
    25021 aaacgattgc tcaagtcagt gacgcaggaa tgccttccat cagcgatcct ggacatgagc
    25081 tagttttagc ctgcataaaa gaagggattt ctgtgatctc tatcccagga cctacagctg
    25141 ggatgacggc tttgattgct tcagggctag tgcctcaacc atttttattt tatggcttcc
    25201 ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa gctaaaagaa cagacagtta
    25261 cgctgatttt ttatgagtca ccttatcgga tcactgctac attgacgaat ttcttagaaa
    25321 tttttggaaa tcgccaagtc gttttgtgtc gggaattgac aaaggtccat gaagaatacc
    25381 ttagaggaag tacggaagag ctactcgctt atatcgaaga acatccagta aagggtgaat
    25441 gttgtctgct tgtagaaggg aatttgttcg aaatgccagt gacggaagtt gatgaagagt
    25501 tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc aggcgaaaaa ccaaatgctg
    25561 ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca agaagtctac cgtcagtacc
    25621 atgaattaga ctgagatagt ataagttgtc tataatgaaa gactaatgag gaaatcagct
    25681 actaactttt caaatctctt gaacgcgaac gaatgttctt gtataatgaa taagaggtga
    25741 ggatgatgga gctgcaattt cctttacata atgatacatc aagaaaaatc attcatattg
    25801 atatggatgc ttttttcgct tcggttgaag aacgagatca tcctgagttt cgcggacatc
    25861 cattagtaat tgccagacac cccagtgaca ctggtgggcg tggagtagtg actacagcaa
    25921 attatgaagc aagaaaattc ggaattcatt cagcaatgag tgcacaaaaa gcgtatgagc
    25981 tttgtccgca agccatcttt aagcctggcg accatcataa atacagcgat atttccaaac
    26041 aagtacggga agtattctat cgttatacag atattgtgga accagttagt atcgatgaag
    26101 cgtatcttga tgtgaccgaa aataaaatca attgcaagtc agccatcaaa atcgcccgaa
    26161 tgatccaagc agatatttgg gagagtgtcc agctaacatg ttctgcggga gtaagttaca
    26221 ataagttttt ggccaaatta gcatcagact atcaaaaacc tagaggaatc acggtagttg
    26281 cccctcaaga cgctgtgcca tttttaaaag ctttgccaat tgaaaaattt catggggtag
    26341 ggaagaaaac tgtacctaga atgcatgagc tagggattta tacaggaaaa gacctatatg
    26401 aatgcacaga aatgatgttg attcgaaatt ttggaaaaat gggctattct ctttatcgta
    26461 aagtcagagg aatccatgat tctccagtaa acgttacaag agagcgaaaa tctgtgggaa
    26521 aagagcatac ttatgggcag ccgttgcaaa cggaagaagc agtgctgact cagttgcgcc
    26581 aactagctga aaaagtagaa gaagcactga gaagagtcca aaaacatggg aaaacagtag
    26641 ttttgaaggt caggtacacg gactattcta ctgtgaccaa aagagtgaca ttgccagaat
    26701 atatctataa aaaagaagca ctcttttatc aagcaagcct tatatgggag gaaatcttgg
    26761 gagtagaaaa aggaatccga ttgttaggga ttactttgac gaatctggac ccgatgactt
    26821 atgaaaacat cgtccttcca ttatgggaaa atcaagaaat ataaaaacag ctttgagagt
    26881 tgaaaccgga aatccgtttt ctctcccaaa gctgttttta tatcaagtct tgctttttga
    26941 tttgttcata ggtttctgtt gcatccgtac atttttccca aatcacaact tgattggatt
    27001 ctaaagattt ttggacgtca atgatccgct ggttgctact tccgcgaaat tgtagtttta
    27061 ggtcacgttt gcttaattca aagcgtccgt caacaaggat atcgatttgc gaaaggagtt
    27121 ctaatttatc ttctgaatct tgtagcaatt catcaaaggt atagcctgtc catgaccaga
    27181 tatcttttgt cgtaccgaac tcttttcgga tacgtcgtac gacttgcaga caaacatctg
    27241 tattcaaaaa aggttcgcca ccaagtaaag ttaatccttg cacgtaatcg ttgcgcaagt
    27301 cagcgatgat ttgttcttct aattcaggtg tgaatggttt gccatatcgg aaattttgtg
    27361 cagctttgtt gaagcagcct tcgcaggcaa ataagcagcc actaacgtaa agactgttgc
    27421 ggacaccttc accatcgaca aaattaaacg gtttgtagtc agcaatatag ttttggctgt
    27481 agtcttttgc taaccattct tttggttttg gatttctcat aaaaaaactt ctttcattta
    27541 aggttcaaag atatagctat tgaagataaa atctactcga ccacataaat aagaacaaac
    27601 tgacctaaat ccattgttca agaaagcttg ttcttatttt gatagagggc gttcaaagat
    27661 acaaagccat cgaaagaggg tggtgaaaaa agacttgtca tagcctctta ttctgaataa
    27721 acggtgttca aaagcttgca tctgcagtaa taagcccaac taaacgaaaa atatgaggaa
    27781 ctatttttgt ttagctattc ttattacttg atgcaggaca gctttttcac aacctcattt
    27841 catgtgtttc acacgtgagg aaatttcttt atggcggcca tgtaccatcg gacgagcttg
    27901 cggatttcct aaatagccac aagtacgttt gactacatca catgtttttg gatcatgatt
    27961 tccacattct ggacattcga accctcgttc tgtcggtttg aaatcacctt cgaaaccaca
    28021 ttgatagcaa tgatcgattg gcgtattcgt accaagatac ccgattttgt catatgcaaa
    28081 atcccataca gcttctaaag ctttcggatt ttgttggagc atagggtatt cacagtagtg
    28141 gatgaatcca cctgaacaat attttggata atccttctca aattccaatt tttcaaatgg
    28201 cgttgggttt ttacgcacat catagtggaa actgtttgta taatattctt tatctgtgat
    28261 attttcgatg atgccgaatt tttctgtatc caaacggcag aaacggtcag ttagactttc
    28321 acttggagtt gaatagacac tgaaatgata accatattca tccccccatg catctgcatt
    28381 agcttttaat tctttgacga tatcaagtgt gaaagctttt gcttctgggt ttgtttccca
    28441 ttcgccaccg aagaaagctg aagcaacttc atacaatcca atgtagccta acgatacagt
    28501 agctcgacgg tttttgaata attcattgac agaatcggta cgtgaaagac gtttcccgaa
    28561 agcaccatac atatataaaa ttggcgcatt tgctggaatg gcttctttac aacgttctac
    28621 acgatagaga agcgcatctt tcatgatttc taagcggtca gacagtaact gccagaattt
    28681 attctgatct ccttttgatt cgatcgcgat tcttggcaaa ttcaatgtaa caacacctaa
    28741 attcattcgt ccgacattta cttcttgacc attttcgtct ttccaccctt gcaagaatga
    28801 acgacagccc ataggtactt tgaaacttcc agttaattca acgattttat cgtaattcag
    28861 aatatcagga tacattcttt tcgttgcaca ttccaatgct agttgtttga tgtcgtaatt
    28921 aggatcttca gggtttaagt tcacaccttt tttcaaagtg aagatcagtt tagggaagat
    28981 agctgtccgc ttttcactcc ctaatccatt gattcgaatc tgtaaaattg ctttttgaat
    29041 ttctctttca aaccagtttt ctcctagtcc aaatcctaat gaagtgaaag gagtttgtcc
    29101 atttgaagta aataaggtat tgatttcata ttccaaactt tgcatagcat cgaagatatc
    29161 ttttttggtt tttgcttttg catattcttt ttggcgttct ggcgaatcga tccattcttc
    29221 cgcgtctttt agatgttttt tatagttcaa ttctgcaaaa ggagcaagca attcatctgt
    29281 tcggtcggca gaacagccac catattgact tgacgctaca ttggcaataa tctgtgagat
    29341 ttgtgctgtg gcggtctgga tagattttgg cgattcgact tcggcattgc cgattttgaa
    29401 cccattgttc agcatgcctt tgaaatcaat caagcagcaa ttcgtcatcg gtgtataagg
    29461 atgataatcc aaatcatggt aatggatatc gcctttttga tgcgcatttg ctatatgtgg
    29521 tggcaacatt ttcagtccga ttgattttcc aacgatcccg gctgtcagat ctcgctgtgt
    29581 attgaaaaca ttactgtctt tatttgcgtt ttcgttcacg acagtttggt ccttgttgat
    29641 tagcttttca atcgaaaagt tgatatctgt tgctttactg cgagcaaaat ctcgtttggt
    29701 acgataattg atgtactctt ctgctaattc atattcgttt ttttctagta atatatgttc
    29761 cacgatgttt tgaatctcat agattttgac attttcagca aaccggcttt tgatttcttg
    29821 gacaaccaag tctgcaatca tttgaatttt ttcataagta agtggtgata atgagccgtg
    29881 aattttcttt tctgctttga tcagtgcttc attgatcttt tgttcatcga aatcaacatg
    29941 gcggccatcg cgtttgatga ctttcatctg ggatgtctct ggctgctgag taacgacagt
    30001 tttttcttca taatttactt tcattatctt caactcctgc taaattgttc ttgtctatca
    30061 taaaacaaaa tcggggaagg agcaactaaa tatcggctat atatagtatt caatgtgctg
    30121 tataattgga tttctatact atatgtagtg tatattttat tgtgaaaaaa ggtgaaaact
    30181 tgtagctggt ggatttgttt tttgcatatt taattttgct gaataaaata attgaataaa
    30241 attttttatt cagtccaagt aaaagtgaca aaaacataag agcaaattat tcgtagatat
    30301 gctactatgc aaataaatag aaatgaaaaa tgaaagagga ggagaagaat tggaaaagat
    30361 gatcgaagtg aaacatctaa gtaaaagcta cggcagcaaa ctttctcccg tcaaagcctt
    30421 agataatatc tcattcacag tagaagaagg cgagtttgtt gggattatgg gcccaagtgg
    30481 tgcaggaaag acaacgctga tgaatatctt gtccacgatc aatttgccta cgatgggcag
    30541 tgtctcgatc caaggaaaag aaatcacaaa aatgaaaaac agtgagctca gtgactttcg
    30601 tcgaaaaaaa ctaggattca tttttcaaga gttcaatttg atcgataccc taaatgcaaa
    30661 agacaatatc ctattgcccc tagcagttga acggatgact aaagaggaaa tggaaaagag
    30721 agtaagacat gtagctcaat tgttgaatat agaagaactg ctcaaacggt atcccgatga
    30781 actttctgtc ggacagagac aacgaattgc agctgctaga gcacttgttg ttcagccaca
    30841 agtgattttt gcagatgaac caactggctc tttggactcg aagtcggcta cagaattgtt
    30901 gaactacttg aagaccatga atcaaaaaga aaaagcaacg atactgttag tcacccatga
    30961 tccatataca gcaagctact gcgatagaat tttatttatt aaagatggtg tgattttttc
    31021 tgaagttgtc agaagaggga caagaagaga gttctttgag aaagtaatag acatgcaggc
    31081 gacgattggc ggaggaggaa aaatgaatgc tgtataaact agccgttaaa agtttttttc
    31141 ggcagtcacg aggctatctt gtttattttt tcagtttgac tttatccgta atgatctatt
    31201 attccttcag cgcaatgacc tacgatcaat ctttagtcag aagagcaagt caagatactt
    31261 cgatagatgg tggattgagt tttggtggat ttatcattac tgttgttttg ctttttttcg
    31321 tattcagtgc caaccgattt ttcttgaata gaaggcaaaa ggaaataggc atctatcagc
    31381 tttttggtat gaataaactg cagatatcag gtatatatgt gttagaaatc atgataattg
    31441 gattgttcgc ctgtatttct gggatattac tcggtattat tttttccaaa ctcttttcaa
    31501 tgattttggt tcgaatgatg gatatggatc ttactagtcc attttttatc tccattccat
    31561 cagtagtaga tactttattt gcttttttca tcatcctttt agcagtatct gtatattcaa
    31621 tatggaaaat atggcgctat ccaatgattc gaagttttgg agaaaaagaa caagcagata
    31681 gttcaacaat gcgcttcagg acaaggcacc gattactcgc agtcatcggg ttactacttt
    31741 taacgagcgg atattttgga gctagtcatt ttagggaaat cacttcatgg atgatcagtt
    31801 atcattatct tgatacatta gttttctttt ttcctttttt aatttttttc gtttgtgtgg
    31861 tcggtactta tttgtttttc tgttatacat ttcggttatt tttgatcata ttgagtaaat
    31921 ggaagattta ttactatggt atcaattttt tgataatggg taacacgcaa gttcatttgc
    31981 tcaaaagttg gcgtacaaat tctctgatta cagtcattct cggaatttct ttaatgacaa
    32041 ttggcggcac ggctagtatc tctactattt tatcctacca ggagaaagtt tcctccccga
    32101 tggactatca gtttgatgtt gagacagaga aaaaaatcaa gccgattcta gcagaggaag
    32161 ggcagaagat aactaaagaa atcaccttgc attataaagt cattgggagt atctatagtc
    32221 aaaatctcga agaacctatg tttcaaactg ctaatctgat cacggaaaca caatatcaag
    32281 ccttcagaaa agttaatcca gatttgccga acatttcttt gaaatcacct ggtcatactg
    32341 ttttgcttga tcaaatgcaa actctttttc ggaatatttc attatatgga tctagtattg
    32401 gactgccaga taatcaagaa ttgaagattc aacaaatgct tccaagtgta ttaggtgacg
    32461 aaacaatggt atatctaagt cctgtactga ttgtagatga taccgttttt gaaaaagcaa
    32521 aaggtgtgga ttatcaagta gtcaatgtag atgtatcagg tggaaataat gaaaaaatag
    32581 atgaacgact gagcaatgac ctgtcgataa agtggctcaa tgctgtttat tattcttatg
    32641 atatcgtaaa tggaagtcta caaggagaaa ttagcacaaa gaaacttccg gatgaccaaa
    32701 tgaaggaagc acaatttagc caagaaagct ctcgtttgaa tttctcttcc agctattcga
    32761 aattgcgtgc agttaatcgg cgaatcggta tcaatatttt tgtcacacta tttgtcggga
    32821 tgatcgtgat tattgctact ggtagtattt tgacagtccg gcaattagca gaagcagagg
    32881 aagaaaaaga aaattatcac ctattgacta agctggggat tccacaaaga aggatcagac
    32941 ggatgatctt cgagcaaaac gcacttacct tctttccacc catgatatta gggatcactc
    33001 acgcagtatt tgcaatcaat gtcttcactc agtatataaa aactgccgat tactggttgg
    33061 cttatcttgt gagtggctta ttgatcctta tctatctttt gctttatgtc attacgagtc
    33121 agttatattg tcggattatc gaggaatgat atacggaata agacaagata ataaatagaa
    33181 ctaaattttg aataccattt atttggaata agaggttgtg acacgatttt tgtcacaacc
    33241 tctttatttt ttatattttt taaaagtacg agaaattata aagaaaaaag cagtgatttg
    33301 atgtgctatt tttataaaag ctatgtgaca aaactgttag taaaaaaacg gtttctgtaa
    33361 gctagacaca tgtttctttt tgtcaaaatt agttacactt tgtgtgaggt tatatgagga
    33421 gggaatccaa tgaacaaatg gcgttctaat ccaaaattga attattgggg aaaattcatc
    33481 attcgaacaa ttttttatag tgcagtactg ctgttcctga tttatctgta tcactataaa
    33541 aatattcaag gaggaacttt tatctataac gaattttagg aggaagagta tggaaatcaa
    33601 aacgattatt gaagcaatcg atgattgggg aataaatgaa cctgatcgag ttgcttatca
    33661 ggcggatgag acgcatactt ttggtgaatt gaaacaagca tcagacgcac ttgcttatta
    33721 tttagaaaaa aaggtagaag gcgatggacc aattgtcgtt tttggcaatt tggagtttga
    33781 aatgattgtc tcatttctag gggttgtcaa atctggccat gcctatttgc cgattgaaga
    33841 gcatacaccc aaggagcgta tcctatcgat ctttcgggtg gctaaaccat ctatggtcat
    33901 cagtatcggg gactggatag aagatattcc ttcggttcct gtcataacaa aaaaagaatt
    33961 tcaagaaatc aagaagatac cagtaggatt ttatcccaga aatagcgtaa aaggtacaga
    34021 aaatttttat attattttta cttctggtac tactggagaa ccaaaaggcg tacaaatcag
    34081 tcatgataat ctagtcagct ttgttcaatg gacgatggaa gactttggga tcaagcctgg
    34141 tatgcatttc ttagcccaag cgccattttc ttttgattta tcggtatttt ctatttatcc
    34201 tgcattagtt tctggcggaa tgctgaagcc gttaaataaa gcagttgttc aagactttcg
    34261 tcaattattc gcgacattgc cagccttgaa gttgaatgtg tgggtttcta ctccatcatt
    34321 tatggatatt tgcttgatgg aacctacatt caatggagaa aatgtgccgg aattggacat
    34381 gtttttattt tgcggagaag aattgacgaa aaaaactgca gaaagtctac ttgatcgatt
    34441 cccaaatgca cggatatata atacttacgg tccaacagaa gcaactgtag ctatttcaag
    34501 tattcagatt gaccagcatg tactagatag ttatgaccga ctaccgattg gctacgtaaa
    34561 aaaagacacg aaagtttcta ttgtccaaga aggtaaacct gtttcaaaag gagaaacggg
    34621 tgaaatcatt attgcaggac caagcgtatc aaaaggttac ctgaacaatc ccgaaaagac
    34681 agctgctgcg ttcttttgtc aaaatgactt agcaagttac catacagggg atgcaggtag
    34741 actagatgaa tatgggcttt tattttatga aggacgtatg gatcagcagg tcaaattaca
    34801 tggataccgg attgaactcg gggatatcga acattatctg ctccaagaca accgaatcaa
    34861 acaagctgtc gtcgtaccaa aataccaagg aacaaaagta cagcagctgg ttgcttttgt
    34921 cgtattagaa aacaaaacga aacagccgga ttttcagttg actaaatcta tcaaagaaca
    34981 attactagaa gttgtaatgg attatatgat tcctcagaag ttccattatg tcgatctgct
    35041 gcctcagaca gttaatggaa aaatcgatcg aaaaaaatta acagctgagg tgaatcctga
    35101 atgatgttac ctcatatgat cccttatgca gagccgtttt atttttttct tttagccatt
    35161 gcgcttgtac cgatcgttct atctttattg atatgggaaa aaagattacc agtttatcaa
    35221 tcagcagtga cacttttctt tttatttgtc agctttggag gtaattattg gcaacaggga
    35281 gcggcgctga ttgggtatgt tttgtggcag acgatacttg tatggggcta ttttgtttat
    35341 cggcaaagga aaaatcagac aggatggttt tttgccgccg ttattttagc gataattcca
    35401 ctgtttttaa cgaaagtgac tccttttttt gaacatggaa aagcatcgtt acttggtttt
    35461 ttaggaatct cttatttaac ctttaaatct gtacaaatga tcatggaaat aagagatggc
    35521 atgatcaaaa catataagcc atttcgttat atccagtttc tgctgttctt tccaacaatc
    35581 tcttctggac caatcgatcg ctataggaga tttgaaaaag atcagctcca tccgcctgtt
    35641 cgtgataaat atgttgatct tttgggaaaa ggcgtccaaa atatttttgt cggtttttta
    35701 tataaattca ttatcggtta ttattttggt caagtactgc ttcctgtagt gggacgcatt
    35761 gccttacagc atggtggtat ttcatgggca ttagtaggtt atatgtacgt gtacagtatg
    35821 tatttgtttt ttgattttgc tggctatagc ttatttgcag tcggtacaag ctacatgatg
    35881 ggatatcaga caccaatcaa tttcaataaa ccttttttat catggaatat caaagaattc
    35941 tggaatcgtt ggcatatgac gctgtccttt tggttccgtg attatgtata tatgcgattg
    36001 atgttcttca tgatgaagaa aaaatttttt aaaagcaaga tcgtaatgtc aaatatcggt
    36061 tattttgctt tgtttcttct aatgggtata tggcatggat tgacgtggta ctacatcgta
    36121 tatggactgt atcatgcttg tctgatctgt ctgacagatg catggatccg atttaagaag
    36181 aagcataaag agcagttgcc ttctaacaaa tttacgcatg gcttagccgt atttttgaca
    36241 tttcaagcag tatgttttag cttcctgatt ttttcaggat ttttagataa actttttttt
    36301 agctaacgaa tagtgacata ttaatttata taacaaagga gaaaaggaaa atggaagaac
    36361 aaattttatc aatcttagaa gaaataacag gaacagatga agtaagaaaa aatcgtgata
    36421 ttgatctatt tgatgaaggg ttgcttgatt ctttagcatc tgttcaatta ttagtcgagc
    36481 tagacggaga gctggatatc caagtccctg tatct
//
LOCUS       DAYLZO010000012        35153 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.012, whole genome shotgun sequence.
ACCESSION   DAYLZO010000012 DAYLZO010000000
VERSION     DAYLZO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.012"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..442
                     /locus_tag="ACGVJG_000592"
     CDS             <1..442
                     /locus_tag="ACGVJG_000592"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFJ6556575.1"
                     /translation="GREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPHGVACA
                     ALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAVGIPET
                     ISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            569..1327
                     /locus_tag="ACGVJG_000593"
     CDS             569..1327
                     /locus_tag="ACGVJG_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFJ6556576.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            1427..2071
                     /locus_tag="ACGVJG_000594"
     CDS             1427..2071
                     /locus_tag="ACGVJG_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFJ6556577.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            2078..3085
                     /locus_tag="ACGVJG_000595"
     CDS             2078..3085
                     /locus_tag="ACGVJG_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFJ6556578.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            3101..3943
                     /gene="kduI"
                     /locus_tag="ACGVJG_000596"
     CDS             3101..3943
                     /gene="kduI"
                     /locus_tag="ACGVJG_000596"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFJ6556579.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            3963..4766
                     /locus_tag="ACGVJG_000597"
     CDS             3963..4766
                     /locus_tag="ACGVJG_000597"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFJ6556580.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5015..6079)
                     /gene="fni"
                     /locus_tag="ACGVJG_000598"
     CDS             complement(5015..6079)
                     /gene="fni"
                     /locus_tag="ACGVJG_000598"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFJ6556581.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6076..7161)
                     /locus_tag="ACGVJG_000599"
     CDS             complement(6076..7161)
                     /locus_tag="ACGVJG_000599"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFJ6556582.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(7174..8151)
                     /gene="mvaD"
                     /locus_tag="ACGVJG_000600"
     CDS             complement(7174..8151)
                     /gene="mvaD"
                     /locus_tag="ACGVJG_000600"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFJ6556583.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(8144..9088)
                     /gene="mvk"
                     /locus_tag="ACGVJG_000601"
     CDS             complement(8144..9088)
                     /gene="mvk"
                     /locus_tag="ACGVJG_000601"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFJ6556584.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            9404..10063
                     /locus_tag="ACGVJG_000602"
     CDS             9404..10063
                     /locus_tag="ACGVJG_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFJ6556585.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            10148..11053
                     /locus_tag="ACGVJG_000603"
     CDS             10148..11053
                     /locus_tag="ACGVJG_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFJ6556586.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(11055..11687)
                     /locus_tag="ACGVJG_000604"
     CDS             complement(11055..11687)
                     /locus_tag="ACGVJG_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFJ6556587.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    11838..11963
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            12007..12531
                     /gene="infC"
                     /locus_tag="ACGVJG_000605"
     CDS             12007..12531
                     /gene="infC"
                     /locus_tag="ACGVJG_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFJ6556588.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            12603..12803
                     /gene="rpmI"
                     /locus_tag="ACGVJG_000606"
     CDS             12603..12803
                     /gene="rpmI"
                     /locus_tag="ACGVJG_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFJ6556589.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            12856..13215
                     /gene="rplT"
                     /locus_tag="ACGVJG_000607"
     CDS             12856..13215
                     /gene="rplT"
                     /locus_tag="ACGVJG_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFJ6556590.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            13467..14804
                     /locus_tag="ACGVJG_000608"
     CDS             13467..14804
                     /locus_tag="ACGVJG_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFJ6556591.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            14852..16981
                     /locus_tag="ACGVJG_000609"
     CDS             14852..16981
                     /locus_tag="ACGVJG_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFJ6556592.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            16956..17645
                     /locus_tag="ACGVJG_000610"
     CDS             16956..17645
                     /locus_tag="ACGVJG_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556593.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(18053..18666)
                     /locus_tag="ACGVJG_000611"
                     /pseudo
     CDS             complement(18053..18666)
                     /locus_tag="ACGVJG_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(18802..18996)
                     /locus_tag="ACGVJG_000612"
     CDS             complement(18802..18996)
                     /locus_tag="ACGVJG_000612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556594.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(19046..19486)
                     /locus_tag="ACGVJG_000613"
     CDS             complement(19046..19486)
                     /locus_tag="ACGVJG_000613"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFJ6556595.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(19490..20335)
                     /locus_tag="ACGVJG_000614"
     CDS             complement(19490..20335)
                     /locus_tag="ACGVJG_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFJ6556596.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            20484..21902
                     /gene="pepV"
                     /locus_tag="ACGVJG_000615"
     CDS             20484..21902
                     /gene="pepV"
                     /locus_tag="ACGVJG_000615"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFJ6556597.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(21959..22906)
                     /locus_tag="ACGVJG_000616"
     CDS             complement(21959..22906)
                     /locus_tag="ACGVJG_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFJ6556598.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(23129..23479)
                     /locus_tag="ACGVJG_000617"
     CDS             complement(23129..23479)
                     /locus_tag="ACGVJG_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFJ6556599.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            23679..24758
                     /gene="pepA"
                     /locus_tag="ACGVJG_000618"
     CDS             23679..24758
                     /gene="pepA"
                     /locus_tag="ACGVJG_000618"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFJ6556600.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24902..25222
                     /locus_tag="ACGVJG_000619"
     CDS             24902..25222
                     /locus_tag="ACGVJG_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFJ6556601.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            25244..25708
                     /locus_tag="ACGVJG_000620"
     CDS             25244..25708
                     /locus_tag="ACGVJG_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFJ6556602.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25913..26518
                     /gene="ytpR"
                     /locus_tag="ACGVJG_000621"
     CDS             25913..26518
                     /gene="ytpR"
                     /locus_tag="ACGVJG_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFJ6556603.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(26557..27846)
                     /locus_tag="ACGVJG_000622"
     CDS             complement(26557..27846)
                     /locus_tag="ACGVJG_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFJ6556604.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            28274..28753
                     /gene="rlmH"
                     /locus_tag="ACGVJG_000623"
     CDS             28274..28753
                     /gene="rlmH"
                     /locus_tag="ACGVJG_000623"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFJ6556605.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28838..29347
                     /locus_tag="ACGVJG_000624"
     CDS             28838..29347
                     /locus_tag="ACGVJG_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFJ6556606.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            29447..30097
                     /locus_tag="ACGVJG_000625"
     CDS             29447..30097
                     /locus_tag="ACGVJG_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556607.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            30548..31486
                     /locus_tag="ACGVJG_000626"
     CDS             30548..31486
                     /locus_tag="ACGVJG_000626"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFJ6556608.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            31499..32551
                     /locus_tag="ACGVJG_000627"
     CDS             31499..32551
                     /locus_tag="ACGVJG_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFJ6556609.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            32817..33452
                     /locus_tag="ACGVJG_000628"
     CDS             32817..33452
                     /locus_tag="ACGVJG_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFJ6556610.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            33643..34434
                     /gene="msrA"
                     /locus_tag="ACGVJG_000629"
     CDS             33643..34434
                     /gene="msrA"
                     /locus_tag="ACGVJG_000629"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFJ6556611.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            34914..>35153
                     /locus_tag="ACGVJG_000630"
     CDS             34914..>35153
                     /locus_tag="ACGVJG_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFJ6556612.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCYVLFP"
BASE COUNT        11118 a         6356 c         7337 g        10342 t
ORIGIN      
        1 tgggcgagaa aaaatggcat taggacagta tattgcggga atgggcttct caaatgtcgg
       61 tttaggactg gtacacggga tggctcaccc gttaagtgca tggtacaaca ttcctcatgg
      121 cgtagcgtgt gcagcacttt tgccaactgt catgaaatat aacaaagaat atactggaga
      181 aaagtaccgt gagatcgctc ttgttctagg gattaaagga gcagctgaaa tgtctttaga
      241 agatgtaaga gaagcggctt gtggtgagat cgatcggttg agcaaagcgg ttggaatccc
      301 agaaaccatt tctgaattag gtgtaaaaga agcagatatt ccagcaattg cagaagatgc
      361 tttgcgagat gtatgcacac caggaaatcc aagagaaacg acagtagaag aaatcattgc
      421 tttatatcaa agtttgatgt aaaaacgaag cggatcttca acattaaatt gcctgtaaag
      481 agaaatggta gtttatatct ggatatctct tttaaattga tggtatttcc gctataattt
      541 ttcttataaa gaaataggag aatattatat ggaaaataaa aagccttatg gaactgtatt
      601 gatcaaagcc tctaaaatac tagactattt agcggaaaac caagatgtct ctttgcagac
      661 gatcgcaaag ggtgtgggaa tgacctcttc tacagtcttg aaaatactag atactttgtt
      721 gatgatcggc tacgtaaata aaaattcaga gaaaaactat cgtttaggtg caaagcttat
      781 tcgctacgcg aataaaaaca ttgaacaaat cgatttggta gagttgactt taccgttttt
      841 agaacagctg cagcaaaaga tcgatgagac tatccactta ggggtattgg acaacaatga
      901 gatcttgtac gtgaacaaac tagatccaaa acatcaaacg atccgcatgt cttcaaaaat
      961 cggaatcacc cgaccattat acaattctgc gatgggcaaa gccgtattag cagaattcag
     1021 tgaagagcag tacactgatt atcttgaaaa gcatccgttg atcccttaca cagaatatac
     1081 gatcaccaat gcattacggt tgaaaaagga aatccaaacg gtaaaagaaa cccacatcgc
     1141 atttgacgat gaagaaatcg aacgtgatat tttttgtata ggagcctcaa tcgtgaaaaa
     1201 cgaacaaatc attggtgcat ttagtgtaag tatgccgaaa tatcgtttaa cagatgagac
     1261 aaaagaacaa ataatcagtg ccctcaagca aacaaaaagc gaaatcgagc aaagaattca
     1321 aaaataatga aaaaagcgtt tgcaattttt cttttctgcc gtataatatg ggtatagatt
     1381 tccaagttat aaaataaaat ttctaaatag gaaataggtg gagaagatga aaaaaattga
     1441 tattttatct cgattgaaaa acgccggagt gattgcggtg gtacgcggaa aaagcaaaga
     1501 agaagctttg aatgcttgcc acgcaatcat caaaggtggt ctaactggta ttgagttgac
     1561 ttttacagtc cctcaagcag atcaagtaat caaagaactt ttgtcattct acaaagacca
     1621 accagaaata gtgattggcg caggcacagt attagacgct gtcacagctc gcttggctat
     1681 cttagctggc gcagagtata ttgttagccc ttcctttgat caagaaacgg cagagatgtg
     1741 caatctgtat caaattcctt acttaccagg atgtatgact attaccgaga tcaaaacagc
     1801 gctgaaaagc ggggtagata tcgtcaaact atttcctgga agcgcttatg gaccaagtat
     1861 catttctgcc ttcaaggcgc cgatgcctca agtcaatatc atgccaactg gcggtgtgag
     1921 tctggacaat atgaaagagt ggttcgatgc tggtgtcgta acagtcggag taggtgggaa
     1981 ccttttagca cccgcagcta caggagattt tgacaaagta acggaagtcg cgcaacagta
     2041 tgcagcaaaa atgaaagaaa tcaagaggta gctgcttatg ggaaaagtag tgacactcgg
     2101 agaaatcatg ttgcgattgt ccacagagca gggaacccgt atcgattcaa gtgaacgatt
     2161 cgctgcccat tatggcggag gggaagcaaa cgtagcaatt tctttagcta attacggaca
     2221 tgaagcgatt tttgctacaa aagtacctga taacgcattg ggaaaagcag tcaaaaagca
     2281 cttgcaatgt tatggagtag atacgaaaca ggtattatct ggtggatcaa gactaggtac
     2341 ttattatatg gaaactggta gtggcgaacg cgccgcctca gtgatctatg atcgtgccgg
     2401 atcaagtttt gctgaaatgg atcatttgga atggtcgcta gaggagttat tccaaggaat
     2461 agatatattt catgtttcag gaatcacccc agctttgtct aaaaaatggc aaaaaatgac
     2521 gaaacaggtg atagaagctg caaaaaaagc tggctgcctg atcagcttcg atatcaacta
     2581 tcgtggaaaa ttatggacac agcaagaagc cagcgaagta atccatcagc ttttaccttt
     2641 agtagatatc tgctcagctg gaaaaatgga tgctctttat ttgttaggta tcgagaaagc
     2701 gccagaagca gaagaacaac ctctaattta ttattatcaa aaaatgcagg aaaagttccc
     2761 gaatattcag gtgttttatt cgaccaagcg taccgttcat tcagcaagtg aaaatgaatt
     2821 gaaggggaca ttgtggatga accatgaata tgtggaatcc caagtccata gaatcacccc
     2881 aatcgtggat cgagtaggtg gcggagatgc ttttgcaggt ggcgtgctcc atgggatttt
     2941 atccaatatg tctccgcaag aaatcattga ttttgctacg gcagcttctg ccttgaagca
     3001 tacgatccat ggggactgca accaatttag tcaagaagaa gtcgcacaat ttttagcatg
     3061 cggctctgga aaaatcattc gatagaagga gtagaagaaa atgcaagaaa tggaaacacg
     3121 ttatacacac agtccagaag atatccgtca ttattcaacg gaacaattga gaaaagaatt
     3181 tttagtagaa aaggtatttg aaccagggaa aatcagtttg acttatacgc acaacgatcg
     3241 aatgatcttt ggtggagtga cacctactga aaaagaatta gaaatcacat tagacaaaga
     3301 actaggtgtt gattacttct tagagcgtcg tgaactaggt gtgatcaata tcggtggacc
     3361 aggattcatc gagatcgatg ggcaaaaaga agaaatgaaa aaacaagacg gatactatat
     3421 cggaaaagaa acacgacatg ttgtattttc ttctcaagat gctgaaaacc cagctaaatt
     3481 ctatattagt tctgttccag ctcatcataa atatccaaat gtaaaaatca gtatcgacca
     3541 agtaaaacca atggagactg gtgaaggcct gacactgaat gaacgtaaaa tctaccaata
     3601 catccatcca aatgtatgcg aaagctgtca attacaaatg ggttatacga ttttagaacc
     3661 aggaagcgct tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt
     3721 ctactttgat tatgccaatg aagatacacg tgtgttccac atgatgggca aaccagatga
     3781 aacaaaacat ttagttgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca
     3841 ttcaggtgta ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac
     3901 ctatacagac atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa
     3961 tgatggaatt tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg
     4021 ctgtctatgg tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg
     4081 tatttaataa cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag
     4141 gcatcgatgc aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg
     4201 ttcaacagat caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca
     4261 tcaaacggac accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg
     4321 atttaaatgc cccattcatc gtttcaaaag cagttattcc ggatatgatc gaaaaaggcg
     4381 gcggtaaaat catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg
     4441 cttatgctgc ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg
     4501 gtcaatacaa tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg
     4561 ctccattacg cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg
     4621 gacgtacgcc agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct
     4681 tggcttctag cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac
     4741 ttgcctatat cggaaaacaa ccttaaaaga gctttagtac actgtttagt ggagaggttg
     4801 tgaaatcagc ggattgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa
     4861 aaagaggctg ggacagaagc atttaactcc aagaaataag aaggaattca cgaaaattgc
     4921 ttttcaaatt tttgtgaatt tcagcttatt tccgaaggag ttgcttttac ttccgccgtt
     4981 tgtacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctcttttctg
     5041 tgcgtcttcc atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc
     5101 cggaaaagat cagagattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat
     5161 aaagcagacg aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt
     5221 gattcatcag atgcgtcagt acggttcctg aagtacccac ggcatttgcg ccgagacaaa
     5281 gggctttaaa tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag
     5341 tgtctgcttc atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat
     5401 aactcagttc ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc
     5461 gaccgctgat atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg
     5521 tcatgccaaa accgacttct tttacgatga gggggacgtc aatggctgtt tgagtttctt
     5581 gaataagtac tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag
     5641 gtgcattcaa atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tctttggctc
     5701 tttcgacaga agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc
     5761 gcataatcgt ataagtatcg gctaaagaag ggactttcaa ggcagcgctg actgaaccag
     5821 tggcaatcat caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg
     5881 ttttttcgct tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa
     5941 ctgcctgtgt acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga
     6001 tccgtacgaa gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat
     6061 gttcgtcttt tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag
     6121 tggagtgatt tctgcttttt cccatgcact cattaaagga agaatgccag atttctggtc
     6181 aacaattacg attccgcaat cgcccccacc tgcaccagaa gattttgcgg ctccttcata
     6241 ctgttcagct aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat
     6301 tgcagataaa tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac
     6361 attattttct ttaaaccctt tgatcatttc gttgacacat tctgtactat tttttaagaa
     6421 aagctgataa gccaccattt tatcttctct cgaacggtga acttgatcga ccaaatcaga
     6481 agtagaggca gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa
     6541 tggctcaatg gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt
     6601 ttcttgttct tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca
     6661 gctagcagcg atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc
     6721 aatcttgaaa atctccaact gagataaatt caacgcgtaa aaaacattca aggctttgac
     6781 cgttgcaacg gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc
     6841 taactcactt gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata
     6901 cttttcagtc aagcgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa
     6961 aactaattct ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg
     7021 gatacttccg acttttcgtg cagattctac agtgactgtc acgaattgat cgactgcagc
     7081 gataactgct ggatggcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc
     7141 tggtgcagat acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc
     7201 agcatgtgca gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc
     7261 agataaatgg ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta
     7321 gcatggaatg ccttgctttc tcaattgtcg caccgcatcc atcgctttta agctgtcagg
     7381 agaccagtaa gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt
     7441 tctttccatc gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg
     7501 atatagatcc ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt
     7561 ccgacgcatc ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac
     7621 cattgctaaa tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc
     7681 gtcatggcct ttttcccatt cgacgaaacc accgaaaata cttcggcaag ctgaaccaga
     7741 cccgcgtcga gcaaatcttg aaagagagag atcatctaat ccaagcttaa gagcagtatt
     7801 gcaagcgcct gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg
     7861 aacgaaattc tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag
     7921 gtcaagaaac tgactgactt tttttgttgc tttttcgtct tgcaaggtgc catctagata
     7981 aaattcatct accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc
     8041 caatgttaac gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca
     8101 atatttgatt agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta
     8161 ctcctaatcc ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt
     8221 cttgggcttt tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag
     8281 ttaatttggc acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat
     8341 cattgctgat tgtcagcttt tccagaagtg attgtgattc atccattgtc tgtgccaacg
     8401 tttctggtga attttcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt
     8461 ggatcatttg tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg
     8521 cttctcttgt ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca
     8581 aagaaaagta gtcgaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag
     8641 cggctgcatc gattccacta ggattaccgt gggcgatttt ttccgaaagc tggacatttt
     8701 ctaatagtat ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg
     8761 ctgtggcgac tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg
     8821 tcagttgcaa gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga
     8881 tattttttaa tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt
     8941 gatcgtccat tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa
     9001 aggcaatcgc tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc
     9061 ttgattctcc ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt
     9121 taagcaaaaa ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact
     9181 aagggtctca aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa
     9241 gaatacgttt tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc
     9301 tttgactaga aaaaaataca attgacacta ctagatattg ggggtatatt taaaaaggtt
     9361 ggagttaaac acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt
     9421 agcaggtaca attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga
     9481 tagtcaggca ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa
     9541 ccctgaacag tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat
     9601 caaacaagca atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact
     9661 gcttttccat ctaaatgcag atctaggaag agcaacagaa acagaagttc gtgtgtatga
     9721 tgaactgctg gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga
     9781 tttattagtt catattcgtg tatcttttga tacgatgttg gagcgtatcg aaaaacgtgg
     9841 tcgttcgtat gaacaattgt cctttgatcc aagtctatac gattattaca aagaattgaa
     9901 cagacgttac gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg
     9961 agaccgctat aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga
    10021 agaaaaatta gcagagattc gccaaaccaa agtagccatt tgatttacag ttgctcaaat
    10081 aaagggtaaa cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg
    10141 atagagtatg cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca
    10201 gcaaaacgat gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat
    10261 gctgacttat tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga
    10321 agcgttactg cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac
    10381 tagtcctatc gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac
    10441 cgagtacgtg attcgactaa ttcgatcgct tttggaacga atgccagaca tgagtgtata
    10501 cagtttattg tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga
    10561 agtgaatagt gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa
    10621 tctgctgcaa ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca
    10681 aaatgtagta cttttagaac aaatcaaggc attggctatt tatcgcaagt tgtcagaaaa
    10741 ggaaaaagaa gagttggacg atgcgctgat cgaagcacga caagcagttg aaatgacgaa
    10801 cttagcttcg cagattttag accagctctc tggcacttat aataatttgc tgaataacaa
    10861 cttgaatgac acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat
    10921 tgtgacagga ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg
    10981 gggaatcgcc ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg
    11041 gcggattcgt tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct
    11101 tgctcttggt tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgctgtgaag
    11161 aaattagtcg taaagtctaa tcccttcaga gaagttcgaa accagttcga accggtcaac
    11221 tgattgtcag acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat
    11281 tgacaatgat cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca
    11341 atacaatcag tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat
    11401 ttacattgat gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa
    11461 tcacattttt caaaaatgac attactgcat tcaaaatgac gtaaaactgt tttttgcatc
    11521 gtaagttttt ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg
    11581 tcacccccgt tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc
    11641 tttgtaagaa gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc
    11701 gtttttcttt agtatagcaa agaagcggga atcactgcta gaaagtctgc taatctaatc
    11761 attttcagaa aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct
    11821 atactatgat ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg
    11881 tcactgggct agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag
    11941 agaactcgtt tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg
    12001 aggtgaatga ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta
    12061 cgattgatcg gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat
    12121 attgcagaac aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt
    12181 gcgcgaatca tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg
    12241 cgtaaaaaac aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg
    12301 aatgacttca atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg
    12361 aaagcttcta tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc
    12421 ttgaatcgct tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg
    12481 gacggacgca gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga
    12541 ctaactggct gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag
    12601 tcatgccaaa acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag
    12661 gcggattgaa acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac
    12721 aacgccgtca attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc
    12781 aacaactagc aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat
    12841 taggaggaat tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa
    12901 agagtgctaa aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca
    12961 aaagaacaag taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc
    13021 gacttccgta aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc
    13081 tactcaaaat taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta
    13141 gctgacttgg ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa
    13201 gctttggcta aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt
    13261 ctttcctctt tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt
    13321 ctaaaatagc ataattaagg ttttttaatt gaaaactgaa catttatggt caccctttaa
    13381 tgaaagcgct aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag
    13441 aaaaacaaaa tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta
    13501 tctcattctt tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttg
    13561 ccatcatggc tactgtatgg atgggattag cgctagtcgg aaacacgttt tcaccaaacc
    13621 cggcgatgga agggcaagga gtaattgatg tgttgacgca tatctatacg acggggccag
    13681 ctgagtacgc caaatcgatc ttggtaaatg tcttctttgg tgcctttttc gggcgtgttc
    13741 tagtagatag cggaatcgca gctaccctga ttcgaaaggt agttgaactt ggaggggaca
    13801 agccaagaat cacgatgagc ttactatgta ttgtaaccgc tgttatattc atgtcgatga
    13861 caggaatcgg accagtgatt tctattgcag ttattgtgtt gccaatcttg atgtcattag
    13921 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
    13981 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
    14041 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
    14101 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
    14161 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
    14221 cagttttagg ggtcgttctt ttagatcttc caatcatctt aggttttatt ctagcaggta
    14281 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
    14341 ttgcgaaatt atttacagat ggcgcagtag atgttgcacc aatggttggt ttcttgatga
    14401 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
    14461 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
    14521 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
    14581 tattagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaatcacaa
    14641 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
    14701 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggag
    14761 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
    14821 aaaaacgatt agatgaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
    14881 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
    14941 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagccgcag gtgtcgttca
    15001 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
    15061 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
    15121 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
    15181 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
    15241 acacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
    15301 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
    15361 tgaagcagca tcggtaaaat cgatccctac aacgatggct tctgacatca caaaattata
    15421 tggattgaca agaagaactc ggacagcagc aatcaatgca tctattttac caaaaatgct
    15481 agatacggca acctctacag aagattccgt acgagaagca ggggtcaatg tttctttgat
    15541 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
    15601 aaccatgctt tccggtccag cagcatcagt catgggctct ttgatgtact tgagagcgtc
    15661 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
    15721 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
    15781 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
    15841 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
    15901 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
    15961 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
    16021 caatgtcctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
    16081 aaaagccatc caagcattag ctgattattg tcatacgacg gttgaagaca tcgcagaaca
    16141 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
    16201 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
    16261 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
    16321 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
    16381 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
    16441 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacaga cttcaaaagt
    16501 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
    16561 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
    16621 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
    16681 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
    16741 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
    16801 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
    16861 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
    16921 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
    16981 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
    17041 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
    17101 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
    17161 ccgatgtagg agaaatttgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
    17221 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatt cgattgatga
    17281 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
    17341 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
    17401 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
    17461 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
    17521 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
    17581 ggatcttaga agaaataaaa aaaatagaaa aaagatgtac ggagtatgat ttagattaca
    17641 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacgatgttc
    17701 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
    17761 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaacggtgt
    17821 tcaaaagctt gcatctgcgg taataagtcc agccaagtta aaaacataag aagctatttt
    17881 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
    17941 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
    18001 tttgccgttt ctttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
    18061 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
    18121 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
    18181 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
    18241 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
    18301 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
    18361 caaaaaaagc ttttttattt tgagcgatta ctgcctgatc ggtttttcca acttttcccg
    18421 cgtataacgg gctaatgaag cagatcggat atcgattttt gaaacataag tgccggactg
    18481 tggaatggtt tttacaagct cttgattgcg cagttggatc agtgcttctc gaatcggtgt
    18541 acgcccgata gctagttgtt cttctaacac tttatcggag attttacttc caggtgctaa
    18601 ctcggaataa atgatttgtt tacgtataga ttcataagct tgctgttgca aattattgat
    18661 aaccatttaa ccttcctttc tatgcagata taatctatta taacataaat cgcttacaaa
    18721 tcgtgtgggc ataaaaacac agtctgcaaa aaaagccctg ataggcatgc ctattagagc
    18781 ctcgaaaaga aaaatattga atcaatctgg tcgtacctct tgtatcggtt tgacacgacc
    18841 gccgattctt ttagcaaaag cttcggattc ttttttgtct ttgaaaatga gcagattgtc
    18901 taagtttttt tcccgagtgc gacagccagt tttatcgatg taaccgtgca ttactttaac
    18961 gacatacatg gggatcacct gcttttttct tttcattata cgaactactc gacacaatac
    19021 aagaaaaatg cttagacggt ctttattaag aatttacatc atccaaagaa gcgtacgctt
    19081 ggaataactg atctaactcc ggattgactt tgctcagctt cactaatcgt tgcgaatcag
    19141 aaagtaaaaa acagtgttta ctactgccag tagtttttaa ttttttgtct tcagttccca
    19201 caatttcgta gctaaaatcc aaccgtgtcc ccgtatactt ttggatagtt ggatggatgc
    19261 tcactttttc tccgtaatgg atcatttctt tatagctgca atttacttcc agaacgggga
    19321 tgatgatccc agattcttcg atttttttat agggaaaccc tagatgtacc aataaggcta
    19381 cccgagcctc ttcaaaccag cggatataat tgctgtgatg aataatcccc atttgatctg
    19441 tttcataata aaaaggttct cgcacataac ctgtaaattt ctccatgact catgcctcgt
    19501 tttccattga ttttttacaa aaataattca tccatttcgg aagtgcttcg ctgatttgtg
    19561 tcggcaaaac aatatattgt tcttttgcca gctggtctcc aatgaaacta tgaagataaa
    19621 tagctgctgc aatcgtttgg tcattttgag gaaactgggc taaaaatcct gtgatcatcc
    19681 cagctaaagt gtcacccatg ccgcccgttg ccatagcagc agaacctaaa ggattggtgt
    19741 agaagtgctg gtgaccataa atttttgtct catgactttt caaaacgagc acagcaccga
    19801 tttcctgttg cttctttttg ttttctttat cccgttgttc ttgtatcggt agagaactca
    19861 accgttgcca ctccatttga tgaggtgtaa gtacagtttg ttcgggatag ggtagcaaaa
    19921 gatgattagc agcaagtaaa cttaaagcag agccatcaat caccagccat tgttcttttg
    19981 attggttttc tagtacttgt ttgaaacgtt ccaaactctt agaagaagta cccaatccgg
    20041 gaccaatcaa gagtacatct gcttgcttta ttacggaaag aaactggtca gtatccgtcc
    20101 aaactacaat cattgcttct ggcagatgac tgtgaagagc actccgattt ttttcatcag
    20161 ttactacagt cgtgagaccc gctccgctgt gtacggcagc taaagcactc ataatgattg
    20221 ctccgccata ttgttcattg ccgccaatca aaacgatacg tccaaatgtt cctttatgtg
    20281 aaatttttgg acgaggtgtg atgactcttt tcactagttg ttcgtttatc agcatctaaa
    20341 aacctccttt tactcattat agcgtatatt ttttttagag aaaaacagat aagaaaagga
    20401 attgtcagat tttttgaact atttttttct gtaatttgtg gtatcatgaa cacgaagact
    20461 taaataagtg gaggattaac tttatgacaa ttgattggca aaaagaagta gaagcacgta
    20521 aagaagactt gcttgaagac ttgaaaaatc ttttacgtgt caacagcgag cgcgatgact
    20581 caaaagtaac accagatgca ccatttggac ctggacctcg cgacgcattg aaacatatgt
    20641 tagcttatgg cgaacgtgat ggatttaaag taaaaaatgt cgataactat gcagggcata
    20701 ttgatcttgg cgaaggagac gaaacgctag ggatatttgg acacatggat gtcgttcctg
    20761 caggagatgg ctgggacaca gatccttatg aaccagtaat caaggatgga aagatctttg
    20821 cacgcggatc aagcgatgac aaaggtccaa gtatggcagc ttactatgcg atgaagatca
    20881 ttaaagaact tgacttgaaa ctatcaaaaa aagttcgctt cgtagtcggc agcgatgaag
    20941 aaagcggctg gggcgatatg gcttattatt tcgaacatga agaagaacca gattttggtt
    21001 tctcaccaga tgctgaattc ccaatcatca atggtgaaaa aggaaatgtt tctttagctt
    21061 tacgcttcaa aggcgacaat gcaggagatt atgtcttgaa atcttttgtt tccggacttc
    21121 gcgaaaatat ggtgccagga acagcaactg cagcacttca agtaccaagt gcagatgcag
    21181 ctatcgcgat ggaagaagca ttttaccaat tcatcgaagc aaatccagta agcggaacga
    21241 ttgaagcgga caatacgtat gtaaaaatcg aattagttgg taaaggcgca catggtgcaa
    21301 gcccacaatc tggtatcaat gcaggttctt tcttggcatt gttcttagat aactatgaat
    21361 tcctaggatc tgctaaacaa tttatccatg tagcagctgc ttatgtccat gaagattttt
    21421 atggagaaaa actaggagtc gcttatgaag atgaaaaaat gggcaaattg acaatgaatg
    21481 ctggactatt tgcttttgaa gaaaatggta cagaggaagc gaacttcatc aatatgaatt
    21541 tccgttttcc aaaaggtgta acagtagacg gattgcaaag tgacatcgaa caaacagtgg
    21601 ggcaagaagg cgcaacagtt actcgaggtg ctcgcgtaat ggaaccacat tacgtaccaa
    21661 tggatgatcc attggtggct acattattgc aagtctatga agaccacact ggagaaaaag
    21721 gctacgaaca aatcatcggt ggcggaactt acggacgttt gttgaaacgt ggcgttgctt
    21781 atggagcgat gttcccagga tatactgata caatgcatca agcaaacgaa tttatgagtc
    21841 ttgatgactt gttcagagca acagcaatct atgcagatgc catctatcgt ttggcgaaat
    21901 aattaattaa aaagaggatc aagaattgca aattattttt gcagtcttga tccttttttt
    21961 actttctact gtggtattca tgataaacat gatatagatt caattcatat tctttcttag
    22021 cgttcgtgac gactgtatcc aaaatcaacc ctgttactaa tgaaagaagt gctaaagtca
    22081 tcaatccagt acttaaaatc gcagaaggga tgcgtgtaat aaagcttgtc agcatatatt
    22141 cgcgaatcac aggaatcccc gtgaccaatc caaataaaaa gaagaagatg gtccaaatcc
    22201 caaagaaaag caaaggctta taatctttaa acattttaat aatcatcatg atcactttga
    22261 atccgtcaga gaatgtattc aattttgatt cgctgccttc aggacgatca cgataatcga
    22321 ttgggatttc aacgaacttg aattttttat ctaaagcatg gatcgtcaat tccgtttcga
    22381 tttgaaaacc agaactcata ataggaaaac ttttgacaaa gatacgattg aatcctcgat
    22441 agccggtcat gacatcaaga atttttgttt tatacattct agtaattgta tttctaacaa
    22501 ggttatttcc aaaatcgtgg aaaggtctct tattctcatc aaagtaggtt ccatttgata
    22561 ggcgatcacc aatgaccata tctgcttcac cagaacgtaa tttctcgagc aaaccatgta
    22621 cagcttctgc aggataagta tcatcgccat ctaccattaa ataatagtcg gcatcgatat
    22681 caaagaacat ttggcggatc acatttcctt ttccttgacg aggttctttt tttacgattg
    22741 ctccaccttg aacggccaat tcgtatgttc gatcggtcga attattatca tagacataaa
    22801 tatctgcttc tggtaattct cgtttaaagt ctgcaatgac tgttgaaatt gttgcttcct
    22861 cattgtaaca aggaatcaga acagcaattt tttcttgttg ttccattttt cactctcctt
    22921 aactatagaa aacaaaagct aaattaaaaa tacctcataa tcaaaggaaa tgctagtttt
    22981 tcccgctaaa tatcatttat ttaaaaagaa aatactggaa tatatgtttg gaaagctcta
    23041 tctaaaaaac cacccactac aaaagaagga catgatacct ctcttttgaa tagtgggtgc
    23101 gtattcgttc tctcactttt ttgacgtatt aatctgttgc accttttttt cttgaaattt
    23161 ctaatacggt tcctgtatga gcatctgcta aaaattcata ataaacagtt tcgccatctt
    23221 ctattcgaga aagacctccc cgataagcac tcgaccgaac ggcgaatttc ctaataggtt
    23281 ccttttcaaa agagatccaa gaaccttcga ttgcaccttc ttttagaaaa gatgacttga
    23341 tattttcaag tacttggtct gctgacaata cttttttgcg atgataccaa atcgttgaag
    23401 ctaccccgct caataatcct agaccgattc caactgctaa cccgccttta aaatagctta
    23461 gttcgctttc ttcatacatg tggacatacc tccttcttct cacttgttac tccttgtgct
    23521 tattttacca cactctcata ttttctttga aataaataac cgctaccaga aaaaataagc
    23581 tataataaat acgatcggtc tgcagaaaaa atcatatttt atccgcagat caatataagt
    23641 ctgagaggag tttagtctga aatttagacg aatagcatat ggaagaaaaa acatttcaac
    23701 gaatcaaaga gttaacagaa ttacaaggaa caagcggttt tgaacaagat gttcgcgctt
    23761 atatggaaag tcatatggaa ccgctggtag atgagttaca gttagatggt ttgggcggca
    23821 tttttggtct gcgccatcat gaagaagcgg atgcaccccg tgtaatggtc gcagcacata
    23881 tggatgaagt cggattcatg ctgacacaaa tccaagaaaa cgggcttttt aaagtcgtac
    23941 ctctaggcgg ctggaatcct tacgttgttt ctgctcagcg ttttacactt aaaacctcta
    24001 ctggtaagaa ttatccatgt atttcttctt ccgttccccc acatttattg cgaggaacaa
    24061 gcggacaaaa atcagtagaa gtaaccgata ttttgttcga tgctgggttt gaatcaagag
    24121 aagaggcaat gagttacggt gtccttccag gagacacaat cgttccttac gctgaaacga
    24181 tcaaaacagc caacggaaag aatattatca gtaaatcatg ggacaaccgc tatggctgta
    24241 caatggtatt agaagcttta gaagcgttgc aaaatgaaac attaggtcat acattgattg
    24301 caggcgcgaa tgtccaggaa gaagtaggct tgcgtggttc taaagcttct gtcaataaat
    24361 tcaagccaga tctgttcttt gcagtagact gctctgcagc agatgataca gtgacgaaaa
    24421 atggcacatt cggacattta ggagaaggta cattgatgcg tatccaagat ccaggtttga
    24481 tcatgcttcc tcgtttgcgc gagtacttgc tggatatcgc tgaaacaaac aatatccctt
    24541 atcaatactt tgtttcaaaa ggcggtacag atgcaggtgc tgctcataca caaaacgaag
    24601 gaatcccaag tacagtgatt ggcgtagtgg gacgttatat ccatacgcat caaacgatgt
    24661 tcagtattcg cgattttgaa gctgcacgag aaatgttgat tcaaacactg aaaggtttgg
    24721 ataaatcaac agtgaatacg atcgtatacg gaaaataatg cgatagtgaa actataaaat
    24781 ctggcaaaag attgcttttt gccagatttt ttttataaaa agaggatcaa tgtttgttag
    24841 ttcgaaagaa aggtgtataa tttaattgaa aaatacataa acagaaagta gaggacaaag
    24901 aatgattatc ccaaatagtt tagaagaatt agcaggctac gtagaaactg gaaagagtgt
    24961 atttttcttt actgccggct ggtgcggaga ctgccgtttt atcaaaccgc aaatgcctga
    25021 aattgaaaat gacttttctg attggcaatt tattgaagta gaccgtgaca agtacataga
    25081 tgtagccgct gaatggaaca tttttggtat cccaagtttt gtcgtgatcc aagacggaaa
    25141 agaattaggt cgtctagtga ataaagatcg taaaacaaaa caggaaatcg aatcgttcct
    25201 agaaagtgta acaaaaggct gacatctgtt aggaggggtc aagatgaatc aagaatataa
    25261 gaatatttta gtaggtatcg acggtagtga acaagcactt gctgctttca aaaaagcggt
    25321 agaagtggct cgccgtaata atgggaaagt atatgtagca aacgtaatcg atcagcaatt
    25381 ttataacttt atgggctatg cacctattga tcaaaatcta gtcgatcagc aaactgaaac
    25441 agccaaggaa atgatcgaag aatgtaaaga atatgggaaa tctgttgatt acacaaatat
    25501 agaaggaatt attgcatatg gttctgttcg tgaatccatg gcagtcaaat tgccaaaaaa
    25561 atatgagatc gatctaatca tggttgggca gtcaggtcta aatgcagtcg aacgctttat
    25621 gacaggaagt gtagccagct acgtgatacg tcattcacct tgtgatgtct tgatcgtttc
    25681 tgacgaagaa gaacagccaa gtaattaagc aagtggaact tgtacgacct ttttaatcaa
    25741 ttaactaaaa cagtaaaggg actgtgacaa aagtcttgtc acagtccctt gttacgaata
    25801 aatggtgttc cagaagaggc ttcttcggaa ataaagcatg aaaaaaattt catgcagtga
    25861 atgcaatttt ttgttaagat aaatctcagc aaaggataat ggaggaaaga acatgatttt
    25921 tgcttataac aaagcacacg tcggagacac cttgatggtt attatagccg acgataaagg
    25981 aacagaaaac actgttgaac gcaaatggaa tgttgctcgg atcaaagatg aacaaggtca
    26041 agttgttgct tggaactttt tccatatctc cgatcatcta acaatcgaag ggaacggcca
    26101 agtaagctta acagatgagg aattagcgaa attgaatcga ttgatcaaag aagcaggatt
    26161 cgaagaacag cttgaagctg atcatgaacc gaagatcgtt gtcgggtacg taaaaacatg
    26221 cgtcccacat cctgattctg accatttatc gattactgag acagaagtag acaatggtac
    26281 agtattgcaa atcgtttgcg gtgcacccaa tatcgaagct ggacaaaaag ttgtcgttgc
    26341 aaaaccagga gctatgatgc cagatggaat gatgatctgg ccaggaactt tgcgaggggt
    26401 agatagctat ggaatgatct gttccgctcg tgaattgcat ttgccaaatg ctccagcaaa
    26461 aaaaggaatc ttagtattgc ctgaggatgc cgtagtcgga gaagcatttc caaaataaag
    26521 aggttgtgac aagtctttgt cacaacctct tttttattaa ttttcactat tttgattttg
    26581 ttttaatgcc gattgatcga tcgtcaattc aacagtagtt gttttttctt tcgagccttc
    26641 atagtaagtg attttgacag aatctcccac ttttttctta tacaaagcag attgaagttc
    26701 tacaccagaa gaaacttctt tgtcatcgat cttagtaatg acgtcgtatt gtttcaatcc
    26761 agctttttca gccggtgtag ctgtttggac atttgtcacg atgacaccgt tagttacaga
    26821 tgaaggaatc ttcaagattt gttcttgttg ctgagtagaa acggcagata agtcgaccat
    26881 tgtgatacct agagctggac gtgtcacttt tccgtctttt tccagttgat tgatgatatt
    26941 cactacatcg ttacttggaa tagcaaatcc cattccttct acgcttacat tagaagtaga
    27001 ttcagaagta cttgcgatct tacttgagtt gataccgata acttgtcctt cgatattaac
    27061 taaaggacca ccagaatttc cagggttgat tgctgcatct gtttggatcg cattgatatt
    27121 gactgtttcg ttcgattcat tcgtacttgt cacttgtcgg ttcaaggaag aaatgattcc
    27181 tgaagtgaca gaatttgcat attcagatcc tagtggggaa ccaatcgcaa tcgcaggctc
    27241 accaaccttc agagcacttg agtcaccaaa agaggcaact gtttcgactt tgtctgaatt
    27301 gatcttcaac accgctaaat ctgaataagc atctgttcca actaattcag cttttacctt
    27361 cgtaccgtct ttcatcaaga cttcaagacc ttgctggcca tctactacgt gattattcgt
    27421 gactacataa gcagtattcc cgtctttttt gtagatcacg ccgcttcctt cactataagc
    27481 ttccaaatta ctatcatctg agctgctact ttcttcttgc tgcccgaata gtccgccaaa
    27541 accgcttgat tggttttgac tttgtaagtt gataacagaa acgacagcgt cttgaacttt
    27601 atctaccgct ttggtgatat cagaatctac attgactttg acattttcca caactgtttc
    27661 accggcagaa ttctgattgc cgcttgtagc agtagaacta ttgttgccag tgcccatcgc
    27721 tgcataaaaa atgccagctg tcagtagtcc tccaacgatc cctccaacaa gccccagacc
    27781 taacttacgc cagaggctat tatttttact ttttttcatc tttggtgtca cattttttct
    27841 atccatgtgt tcctcctcca attacattca tttatctact gttagtatag tcttatttgt
    27901 tgactttagt ctactttgag actgtgaact ttttatgaaa aactttagga aaaatcattg
    27961 ttttcagatg aaaatatttg attttctttt tccacagata ctgtgtaaaa ctacgcggat
    28021 aggattggaa aaataaggga taaaacatat ccacagcctg tgtataaagt ggataaattc
    28081 ttataaaacg tatgtttgcc taatgaatgt gatgaaataa ggtaaaataa cactgttttc
    28141 tgtggataaa cctgtggatg ttgtgcataa ctgttgggga aatcttttta acaaaaatag
    28201 ccaaaataaa catatagacg aaaaaattga ttataataaa caaagtgagc atgaagacca
    28261 aaggagagaa aacatgaata tcaaaattat ctcagtagga aaattaaaag aaaaatacct
    28321 gatccaaggt atcaatgagt atgtcaaacg gctgaatgcc tatgcaaaaa tcgaattgat
    28381 cgaagttcct gacgaaaaag ccccggagaa tttaagcgaa gcgcaaatga gacaagtaaa
    28441 agaaaaagaa ggggaacgga tcttagcgaa aatcaaagaa caagaatacg tctatgcctt
    28501 agcaattgaa gggaaaaatc ctacaagtga atcttttgct aaacaaatcg accaattagg
    28561 tatccaagga aaaagccacc ttgtttttgt tatcggcggc tctttaggac tcagcgaagc
    28621 cgtcatgaaa agaagcaatg ctcagatctc ttttggaaaa atgacgtatc cacatcaatt
    28681 gatgcgtctg atccttgtcg agcaaatcta ccgtgccttc cggatcaatg cgggggcgcc
    28741 ttatcataag tgatgcggtt tttatcattg atcctaaaaa tcttagcaag acaaaaagta
    28801 cgtaagaaag taaagactaa atacttcgga ggaacaaatg aataacaatg ataggttatt
    28861 gcgattgcgt tatgcgattg atttgaaaga tacggatttg attaaagctt ttgagctagg
    28921 cggtgtacca ctgactaaag aagaagctca ggcggtactg accaaagtac aggataagaa
    28981 caaagataat gcagaaaata atgtgtatga aaaaaccatc aacaaccaag tttttgacgc
    29041 attcctaaat ggcctgattc tcttggcgcg tggtccgcaa aaagataagc cgatagtaga
    29101 cacgccgcaa aaaagcaagg aaatcaaata tatcaacaat gtgttgctaa aaaaattaaa
    29161 aatcgccttg tctatgacca ctgatgatat tttagacgtt tttgctgagg cagaaatcta
    29221 tccatcaaaa ggcgaaatcg gggcattttt gagaaaagaa ggccaacgaa attttaagcc
    29281 ttgcggagat aaatatatgc gtaattttct caaggggtta ggaatttata atcggagaaa
    29341 agtataaaga aatttcatga gaaatcaaaa caatggttgt tttgatgaga gctagttttt
    29401 gtatgagatt aaaatattat cttaatcgag gagctggatt ggttttatga atgatgaata
    29461 taagaatgat gaagataaga tgctttttga agagatagag aatcgatgta ggctcaattt
    29521 tgagcttcgg ggaaagatgt ctttaataca acaaaaaaag tatttagcga ataaatcaga
    29581 gtttactctg ggacatgttg aaaaattaat ctcggattgg attagtagta gaagcgaatt
    29641 tacaaaaata aaacagccga ttaaatttga catgaaaaaa cttttgttaa ataagagtga
    29701 gataggtaat agagatcaat atattagagc taaagggcaa gaaataattg attcattagg
    29761 tgaaatgagg tcatacaatt atttgtatgt aacacataga gctgatggaa tggtcattac
    29821 tgtggggaaa tcttcttcta atgatatttt tttagatgga gatctttttt atcaactcaa
    29881 tacaaatcac ttgtctggca ctgaaaatat aattttaagg acggaatatg gcaatgaaat
    29941 atttgcaaaa tatgatgaga ttttaaaaaa ttatttggac tgggcttgga taattccggt
    30001 tgagtcagga gatgctaaga aactggagag attattgggc gatgaactaa ttaataaaaa
    30061 agtaccaatt ttaaattatt attcccatag acaataaaag gctatttttc atgataatac
    30121 tagttataac tgtaaaaatg gaagaaaatc tgggaaagaa gtttttaaat aagtgtattg
    30181 ttcgatttta ataaataata agcaatacca tccgtctgtg ttgcgcaaca tcgtactaca
    30241 atgaatattt tttcacaaaa agaaaatcat ccatcgaatg aatggaaggt tttagtatac
    30301 acgaggttaa tgccccctct tttataatca gaggagtgag gttttttcta tgaatgtttc
    30361 tattttctta ttgctagtct agtttctctt acgatgaaat tgggattcta ttccagtttt
    30421 tattgatttg ctgataaaat aacttgaatg gtggatataa acatgataaa taagagaaga
    30481 ataacaaaac aaggttgtga aatgattctc aaatgaatat aatagaaaga aaaaggagga
    30541 agaatcaatg aaaatagcaa ttgcaggcgc tggcgcaatg ggaagtcgct ttgggttaat
    30601 gcttcaccaa agtgggaacg aagtattatt aatcgatggc tgggcagaac atgtccaaca
    30661 aatcaaagag catggattac aagcaaattt taatggtaaa gaggtagaag caaaactacc
    30721 aatcgttctt caatccgaag tagaaaaaga agatcaagtt gatctgatta ttctatttac
    30781 caaagcgatg cagctggaaa aaatgctgca ggatatccaa tcattaatca aaaaagatac
    30841 agaggtctta tgtctattaa atggtatcgg gcatgaagat attattgaga aatttgtacc
    30901 aatggaaaat atctatattg gaaataccat gtggacggca ggtcttgaag gtcctggtca
    30961 ggtcaaatta tttggaagtg gttcagtaga attacaaaat ttaggtgatg gaaaagaagc
    31021 agctgcaaaa aaattagcag acaaattgtc tgaatcagga ttaaatgctc atttttctga
    31081 caatattcac tattctattt atcgcaaagc atgtgtgaac ggaacaatga acggactatg
    31141 cacaatctta gacgttaata tggctgagct aggaaaaaca tcgactgctc ataaaatggt
    31201 ggcgacgatt gtcaatgagt ttgccaaagt agcagcagta gagaagattg aactagatgt
    31261 cccagaagtc attgcacatt gtgaatcttg ttttgacccg gaaacaattg ggttacatta
    31321 tccttcaatg tatcaagact tgattaagaa ccatcgatta acagaaatcg attatatcaa
    31381 tggcgcaatt tctagaaaag ggaagaaata tggtgttgcg acgccttatt gtgatttctt
    31441 gactgagctt gtccatgcaa aagaagatag tttgaacgta aaataaagga gagattgaat
    31501 ggaaacttca caaaaaatat caccgaaaat ttttctgaat aaagtcctcg ctggtacagc
    31561 aacaggaatc attattggat tgattcctaa tgcagtatta ggtgccattt taaaatattt
    31621 tagtgcgtat ccctttgcgg taacgatcgc acagttagca gttatttttc aactagcaac
    31681 acctttgatc attggtggtt tgattgcctt acaatttgga tttgatccga tgagaatgat
    31741 ggttgtagcc ggagcttctt ttgttggttc tggtgtagtc aaattcacac ctgatttggg
    31801 tgaaacgggt gtttacgttg gtgctggtac aggcgatatt attaatacga tgctgacagc
    31861 atcgattgct gtcgggctga ttttactgat tggaaatagt tttgggtcag tgtcgattgt
    31921 gttaacaccg attgttgtag gtgtaggtgc aggtttactt ggtttttatt tattcccttt
    31981 tgtaacagca attacttctg caattggatc attgattaat aacttcacta ccttacaacc
    32041 gctgttaatg tctatcttga ttggttgttc ctttgccttt ttgattatct ctcctatttc
    32101 aacagtagca atcggaatgg caatccaact agatggtgtt tctgctggtg cggctgcgat
    32161 gggcgtagct gcaaccactt ttgttttagt agtgaactct tggaaggtta ataagtcagg
    32221 tgtgacaatt gcgattgctt taggcgctat gaagatgatg atgcctaatc tatttagaaa
    32281 acctgtgatt cttattcctt gtttagttag tgcagtgatt acagctattc ctgtcgctct
    32341 attttctatt tcaggtacgc ctgcttctgc tggtttcggc gtggtcggac ttgttggtcc
    32401 gttggcttct ttagatgcag ggctaaatgg ctttcttgtt gttctttgtt ggttagtcat
    32461 ccctgttttt gcggcactaa ttactcaatt tttatgcgag aaagtattta aactgtatga
    32521 tcgagaagaa gtcttcaaat ttttaggcta gtagatttta ctaagaaatt ttcaatcaat
    32581 ggatgcgtaa gtgagatgtc aacaacatct gacttacgtt tttttgcctt tttgagtagg
    32641 agattgatgc tgacaactga actcttcagt gccattcgat tttttaaaat gtagaagaaa
    32701 ctcaccttta tgttattgat tgttcaaaag gcaactagaa taaaaatatg tgttaaaatg
    32761 agaaaaaatt tgattgttgg atgtatcgaa taatcactaa aaagtaggag aagaaaatga
    32821 tttttttgca aaaatatggt ttttattttt tattattagg tgtgattagt gatttatcga
    32881 caccgtatat ccttggcctt ttttatccca aactgaatca aatgactacc gtaattagtg
    32941 tgtttggtga cgtagatagt ccagtcagac gtgctttctt ggtctggtca gttgtatctg
    33001 gacttttttt tgttctttcg ttgcctgcgt tgtatcacct atttattgga acatcaaaaa
    33061 cacttgcaat acttgctgtt gcaacagttg gtttatatgg gataggtgat tgtattttta
    33121 ctgggttatt cagtatcaat acgaatgaaa gctcttggaa tctatcgact tggatccata
    33181 atataggttc tgggctaggc tatgcaggct ttctactttt cccattgtta cttgttctgc
    33241 tttatcgtca aagtggtcaa ggtacgctta gccattttta tcttgtttta acagttatta
    33301 gtttactaat tgcaggtctt tacggattag cacggattcc gtcgattagt cagtttgcct
    33361 tctttaagca attgggcttc tttcaacgct taagtttttt ctttaactat ttgccgatga
    33421 tttgtttaag tttttttcaa ctacgaaagt agaaaaagca gtgtgaagct gattaagtaa
    33481 aagaatttct ctatgggtaa ccattcgtgt ggatgtgttc gactaagaat agagatgagg
    33541 agagcgaaaa gcaagttagt cttaacttgt tatgcgttgt gctaaatttc aggtagtcat
    33601 tttttgaatg aataagaaga aaacttgaaa gagggtgact tcatgttaga tgtggagcag
    33661 atggaaatag acctttataa tattatttta aatcgaaata caagagaatg ggaaagaaca
    33721 caatttgaag aggcaaaaga agacctagca aaaatgtcga taaagcaggt gatagaaaaa
    33781 ctagaggtca acttgcgtcc gctagcacta cgagataatt tgacaccaga tgcgatggat
    33841 ttttatttaa gaatcacagg ggagtcagcg agtgcaaagg cttatgatta tgccaaacat
    33901 aagataactt cacccttata ccaagaacgg gcgatttttg ccggtggctg tttctggtgt
    33961 atggttgagc cttttgaaac aaaaccaggt gtaatctcag tgttatctgg ttatacaggt
    34021 ggagagacca aaaaaccaaa ttacgatcaa gtcagtggtg gatatactgg gcatgttgaa
    34081 gctgttgaga tcatatttga tacacgtcaa ataacctatg aagagctgtt ggagatttat
    34141 tggcaaattt cagaccctac agatgccttt ggtcaatttc aagatcgggg gaaacaatat
    34201 cgttcaattc tatttgttgt taatgaaaag caaaggcaat tggctgaaaa atcaagagaa
    34261 caactgattc aatctaaccg atttagccgt cctatcgtta cagagattcg ccaagcaaag
    34321 actttttggc cagctgaaaa tcatcatcaa caattttatc agaagcaacc taaaagatat
    34381 aaaaagatca agcattcgca tcagcttcat ttattttttc agcgacatag atagaggaag
    34441 gttgtggcgc aaagcgttca gctccgacaa aataatgagc aactgacaaa aatagctttt
    34501 catatttttg gacagttatg aattatttcg aggagttgct ttgagaacac cgaggaaaag
    34561 gttgtggcgc aaagcattca gcttcaagtc gcaaggaaac aggaggtatg acaagtcttt
    34621 tgtcacaact cctgtttttt tactagcctt tatttccatg tgaaagagaa ttcgaattgt
    34681 agaatcttga tttttctatg gaattttgta gagaatcatc ttgataatta atagacacct
    34741 aatcgaattc cacattaatg atgaactaaa ttccgtataa aagcgcaaac aataactata
    34801 aaacccgtgg tgctagttaa ttttttccta gaggaaaatg tctaaacatg ttggtaaatc
    34861 aacaaaaaaa cgcatatagt agtctcaaaa ctcccaagaa atgaggacta catatgcgtt
    34921 catcaaaata tactgaacat tatacagata cttttataac attcaaggaa attatgagaa
    34981 ctgtttctaa catataccac aattgtgtac cagataagat taaaaaccga agaaatactg
    35041 atcaactgaa gcaacgcgat acagtgatta ttgcttgtgt tatatggggc ataattaatg
    35101 gctatactag ccaaagagcc acgtatagag cggtttgtta cgtcttattt cct
//
LOCUS       DAYLZO010000013        32533 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.013, whole genome shotgun sequence.
ACCESSION   DAYLZO010000013 DAYLZO010000000
VERSION     DAYLZO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.013"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            131..505
                     /locus_tag="ACGVJG_000631"
     CDS             131..505
                     /locus_tag="ACGVJG_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFJ6556613.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACGVJG_000632"
     CDS             complement(619..1248)
                     /locus_tag="ACGVJG_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFJ6556614.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACGVJG_000633"
     tRNA            complement(1382..1454)
                     /locus_tag="ACGVJG_000633"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACGVJG_000634"
     tRNA            1668..1741
                     /locus_tag="ACGVJG_000634"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACGVJG_000635"
     CDS             complement(1879..2043)
                     /locus_tag="ACGVJG_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556615.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACGVJG_000636"
     CDS             2338..3666
                     /locus_tag="ACGVJG_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFJ6556616.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACGVJG_000637"
     CDS             3868..4701
                     /locus_tag="ACGVJG_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFJ6556617.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACGVJG_000638"
     CDS             4717..5454
                     /locus_tag="ACGVJG_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFJ6556618.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACGVJG_000639"
     CDS             complement(5609..6577)
                     /locus_tag="ACGVJG_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFJ6556619.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACGVJG_000640"
     CDS             6809..7642
                     /locus_tag="ACGVJG_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFJ6556620.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACGVJG_000641"
     CDS             7676..8515
                     /locus_tag="ACGVJG_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFJ6556621.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACGVJG_000642"
     CDS             8541..9101
                     /locus_tag="ACGVJG_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFJ6556622.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACGVJG_000643"
     CDS             9300..11021
                     /locus_tag="ACGVJG_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFJ6556623.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACGVJG_000644"
     CDS             11186..12328
                     /locus_tag="ACGVJG_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFJ6556624.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACGVJG_000645"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACGVJG_000645"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFJ6556625.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="ACGVJG_000646"
     CDS             14128..14775
                     /locus_tag="ACGVJG_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFJ6556626.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="ACGVJG_000647"
     CDS             15168..17813
                     /locus_tag="ACGVJG_000647"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFJ6556627.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="ACGVJG_000648"
     CDS             18123..19436
                     /locus_tag="ACGVJG_000648"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFJ6556628.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="ACGVJG_000649"
     CDS             19426..20079
                     /locus_tag="ACGVJG_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFJ6556629.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /gene="radC"
                     /locus_tag="ACGVJG_000650"
     CDS             20134..20826
                     /gene="radC"
                     /locus_tag="ACGVJG_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFJ6556630.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="ACGVJG_000651"
     CDS             20976..21176
                     /locus_tag="ACGVJG_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFJ6556631.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="ACGVJG_000652"
     CDS             complement(21430..23109)
                     /locus_tag="ACGVJG_000652"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFJ6556632.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="ACGVJG_000653"
     CDS             complement(23111..23323)
                     /locus_tag="ACGVJG_000653"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFJ6556633.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="ACGVJG_000654"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="ACGVJG_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFJ6556634.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="ACGVJG_000655"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="ACGVJG_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFJ6556635.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="ACGVJG_000656"
     CDS             27298..27495
                     /locus_tag="ACGVJG_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556636.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="ACGVJG_000657"
     CDS             27648..28133
                     /locus_tag="ACGVJG_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFJ6556637.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="ACGVJG_000658"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="ACGVJG_000658"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFJ6556638.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="ACGVJG_000659"
     CDS             29010..29537
                     /locus_tag="ACGVJG_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556639.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="ACGVJG_000660"
     CDS             29628..30365
                     /locus_tag="ACGVJG_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFJ6556640.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="ACGVJG_000661"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="ACGVJG_000661"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFJ6556641.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="ACGVJG_000662"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="ACGVJG_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFJ6556642.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5522 c         6874 g         9365 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DAYLZO010000014        32388 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.014, whole genome shotgun sequence.
ACCESSION   DAYLZO010000014 DAYLZO010000000
VERSION     DAYLZO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.014"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            1..791
                     /locus_tag="ACGVJG_000663"
                     /pseudo
     CDS             1..791
                     /locus_tag="ACGVJG_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(917..1996)
                     /locus_tag="ACGVJG_000664"
     CDS             complement(917..1996)
                     /locus_tag="ACGVJG_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFJ6556643.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2099..2539)
                     /locus_tag="ACGVJG_000665"
     CDS             complement(2099..2539)
                     /locus_tag="ACGVJG_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFJ6556644.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2596..3006)
                     /locus_tag="ACGVJG_000666"
     CDS             complement(2596..3006)
                     /locus_tag="ACGVJG_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFJ6556645.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3186..3620)
                     /locus_tag="ACGVJG_000667"
     CDS             complement(3186..3620)
                     /locus_tag="ACGVJG_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFJ6556646.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3720..4568)
                     /locus_tag="ACGVJG_000668"
     CDS             complement(3720..4568)
                     /locus_tag="ACGVJG_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFJ6556647.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4957..6723
                     /locus_tag="ACGVJG_000669"
     CDS             4957..6723
                     /locus_tag="ACGVJG_000669"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFJ6556648.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6825..8084
                     /locus_tag="ACGVJG_000670"
     CDS             6825..8084
                     /locus_tag="ACGVJG_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFJ6556649.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8231..9532
                     /locus_tag="ACGVJG_000671"
     CDS             8231..9532
                     /locus_tag="ACGVJG_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6556650.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9535..10386
                     /locus_tag="ACGVJG_000672"
     CDS             9535..10386
                     /locus_tag="ACGVJG_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFJ6556651.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10399..11214
                     /locus_tag="ACGVJG_000673"
     CDS             10399..11214
                     /locus_tag="ACGVJG_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFJ6556652.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11365..12531
                     /locus_tag="ACGVJG_000674"
     CDS             11365..12531
                     /locus_tag="ACGVJG_000674"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFJ6556653.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            12761..13408
                     /locus_tag="ACGVJG_000675"
     CDS             12761..13408
                     /locus_tag="ACGVJG_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFJ6556654.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13492..14436
                     /locus_tag="ACGVJG_000676"
     CDS             13492..14436
                     /locus_tag="ACGVJG_000676"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFJ6556655.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14571..16022
                     /locus_tag="ACGVJG_000677"
     CDS             14571..16022
                     /locus_tag="ACGVJG_000677"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFJ6556656.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16043..16879
                     /locus_tag="ACGVJG_000678"
     CDS             16043..16879
                     /locus_tag="ACGVJG_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFJ6556657.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            16879..17538
                     /locus_tag="ACGVJG_000679"
     CDS             16879..17538
                     /locus_tag="ACGVJG_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFJ6556658.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            17664..18353
                     /locus_tag="ACGVJG_000680"
     CDS             17664..18353
                     /locus_tag="ACGVJG_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556659.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18359..19315
                     /locus_tag="ACGVJG_000681"
     CDS             18359..19315
                     /locus_tag="ACGVJG_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFJ6556660.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19359..19871
                     /locus_tag="ACGVJG_000682"
     CDS             19359..19871
                     /locus_tag="ACGVJG_000682"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6556661.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20127..20675)
                     /locus_tag="ACGVJG_000683"
     CDS             complement(20127..20675)
                     /locus_tag="ACGVJG_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFJ6556662.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(20764..21945)
                     /locus_tag="ACGVJG_000684"
     CDS             complement(20764..21945)
                     /locus_tag="ACGVJG_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFJ6556663.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22181..23689)
                     /locus_tag="ACGVJG_000685"
     CDS             complement(22181..23689)
                     /locus_tag="ACGVJG_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFJ6556664.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            23848..24039
                     /locus_tag="ACGVJG_000686"
     CDS             23848..24039
                     /locus_tag="ACGVJG_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556665.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24242..25159
                     /locus_tag="ACGVJG_000687"
     CDS             24242..25159
                     /locus_tag="ACGVJG_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFJ6556666.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25152..26225
                     /locus_tag="ACGVJG_000688"
     CDS             25152..26225
                     /locus_tag="ACGVJG_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556667.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26373..26891
                     /locus_tag="ACGVJG_000689"
     CDS             26373..26891
                     /locus_tag="ACGVJG_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556668.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            26872..27264
                     /locus_tag="ACGVJG_000690"
     CDS             26872..27264
                     /locus_tag="ACGVJG_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFJ6556669.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27340..28275
                     /locus_tag="ACGVJG_000691"
     CDS             27340..28275
                     /locus_tag="ACGVJG_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFJ6556670.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28408..29187
                     /locus_tag="ACGVJG_000692"
     CDS             28408..29187
                     /locus_tag="ACGVJG_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFJ6556671.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29180..29497
                     /locus_tag="ACGVJG_000693"
     CDS             29180..29497
                     /locus_tag="ACGVJG_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFJ6556672.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            29741..31351
                     /locus_tag="ACGVJG_000694"
     CDS             29741..31351
                     /locus_tag="ACGVJG_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFJ6556673.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31508..32356
                     /locus_tag="ACGVJG_000695"
     CDS             31508..32356
                     /locus_tag="ACGVJG_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFJ6556674.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        10704 a         5789 c         6331 g         9564 t
ORIGIN      
        1 gttgaagaac caacagataa accagaaaat gaaaataagc cagaagtacc gcctacagaa
       61 aatccagacg gagaacagaa accggaaata gaacctggag aagaacccga tacagaaact
      121 caaccagagc cagacaatga atcaaaacct gaaataaccc ctggagaaaa accagatgta
      181 gacccagaag aaaaaccaga tgtgactcca gaaccagata cggattctgg aaaccaaaca
      241 gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa accagaggta
      301 gccccagaag aaaaaccaga tgtaactcca gaaccagata cggattctgg aaaccaaaca
      361 gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa accagaggta
      421 gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag agaccaagga
      481 ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga gagtaaaaaa
      541 tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc acgaatattt
      601 gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt tgctgtcctg
      661 aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt atactgtgga
      721 gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt aaacaaaaca
      781 gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc
      841 agaaaatgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc atactttatc
      901 tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc gggatctttt
      961 tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc aagccccaaa
     1021 cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg
     1081 ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg acaatcaaaa
     1141 tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct atgaaagata
     1201 gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg aaaatcatga
     1261 tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta gtgagcaacg
     1321 tatttaaaag agcgacaaca aattgagctt ctaataccaa accaaatgta ttgacaaact
     1381 tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc
     1441 ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca
     1501 tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca atcgaagcgc
     1561 cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata tattgtaaaa
     1621 caggatctgc atcttttggc tgatgctcgt aaaaattaat aacggactgg atcatgtcaa
     1681 atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata aggtaaacga
     1741 tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat cgctgaatga
     1801 tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg gttgtaatca
     1861 tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga cggcgtattt
     1921 tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa
     1981 taaacacgtt attcattata tgatggaata tgaaaataag aaagagcaaa aagaaaaacc
     2041 gctaaatgac gttatcttat gagttggttt tcctcataaa atcgttcatc tagcagtttc
     2101 agcccttttt ctggtcttga tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg
     2161 tatccaattc ttcctgttca agtggaagat aagacttgat cttttgtgga ataccttctg
     2221 cttgtttttg caacgcagca ccttgagcag tcagcgtaac atttacgatt ctttcatcac
     2281 ccgtttcacg ctgacgatta atataatttt tttctgctaa cttttttagt aagggagtca
     2341 gcgtaccaga atccagacac aaatcatttc ctagctcttt cactgaagtc tttttctttt
     2401 cccataaaac taacatcaca agatactggg tataggtaag attcagcggc ttgaggaggg
     2461 gagtgtagtg tctaatgatt tctttagagg cggcatataa aggaaaacat acttgattaa
     2521 ctaaatatag atcttccatt tttccacctc cgctgtttta ctatagtgta tcatagcgga
     2581 tttacacaaa aaagtctatt cactaactgc tttcagagta acttctatat ttccagctgt
     2641 agcttttgaa tacggacaca ctttatgcgc tgcctctaat aattcttgcg tttcttccag
     2701 agaaagacct tcgatagatc cttcaatctc tacacctaag acaaagctcg tttcactttc
     2761 tgaataaagg gaaacttgtg cactgacagt agaagcccca gagatatttt tttgctgttt
     2821 gatcaattct aatgcactgt taaaacatgc actgaaacca gctgcaaaca attgttcagg
     2881 gttggttgca ttttcgacgc gtttaccagg agctgcaata ttcaattcga atgtacgatc
     2941 gggattatgc acttctccag agcgtccgcc tgtgttgatc attttcgtag aatacatttt
     3001 tttcataaaa ttaccacctt taattatatt ttatacaatt gaattttaca caatataatt
     3061 gtggaaagca aagaaatgga ttggcataag ataaataaga gaaagcacat gaaacagacg
     3121 cgctttctcg aatagcaaac atatacaaaa aatatcagag acaaaaaata atctcatcat
     3181 aaacattaca tatatttctc aaaaagttgt tttctttctt caaaaattac ttcacgatgg
     3241 acctgatgaa gcatccataa ataaccaaaa ggatctctga acattgagtt agtcacacca
     3301 aagtccatca tttctgtcat ttcttgaatc tctacacagc catttcgtaa tgctttatta
     3361 tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga tcgtatttgg atgttcagca
     3421 gtaggagcag tcaaaccaag agacgtgttt tcatccagca tatggaatct cgtaccatac
     3481 aaggtaaaaa tgacttcatt attgcctcta gggtaatctg ttgcctcaat cagttgtatt
     3541 tcatcaaaga ttgtttgata cagttctagt gctttcaaac tatcatttaa tacaaaatcg
     3601 atttccgttc ctttatgcat aaagatctcc tctccttttc agtcgaacag ttactagatt
     3661 cttttattat accagctaaa gaaaacgcat tcaaaaaaat agcggattta cttaccaact
     3721 cattcgattg gatgataagt aaatccgcgt cccattactt ctgctacgct gctgacaatg
     3781 acaaaggcac ttggatcgat ttcttctatc aatggtttca ttcgggaaaa ttcatgatca
     3841 gtcacaacga tcaataaaac ctcttttgac tttcgattat acccgccttt tgcctctagc
     3901 agggtaaggc cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc caaataatgt
     3961 tcactcatga ccataacaga tttctttcga ttgatacctg tttcgatata agtcatgaca
     4021 attgaagtga ttgcaataga taaaatagca taaagaaact cttctattcc aaaaataaac
     4081 agattaagag aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt attcaagtgg
     4141 aaatattttt taaaaatgag gggagggatc gtcgtaccgc cgctagaaga gttgttttta
     4201 tataaaatcg caactcctaa agcaaaaatc gcacttccaa aaatgacaga caacaaccga
     4261 tctgaagtaa tcattgtttc tggaactatt gcaatgatta aaggaaaaac caagcttccg
     4321 aaaagtactt taaaaaaggg ttttttacct aaaaatagaa gagctagtac caacataacg
     4381 atattcaaca ctaaaataac tatagctcgg tcaaagccta aagcagcttc cagtaatatt
     4441 cctagaccgc tgacacctcc agcagcaata tgatgaggcc ctaaaaacat attgatagcg
     4501 actcctaata tgaagagggc aaaaataatc ttaatgtatg tccatataaa ttgtctcgtt
     4561 tctttcattt attcacttcc ttaattcatt aataatactc ttatcttatt ataacttact
     4621 tttcacctta agttaacttt aattaataaa aaaattaggc tagtctaaat tattatgttt
     4681 cattcttcta gaatatactt ctacgccaaa aactgttaaa aaacttttac tttagaaaat
     4741 tggagaaatt gatcgcttaa gataaattca ggacactaaa tagtacagga agtagactaa
     4801 aacttttaaa agttttaagt ctatctattt ggcaagttta aaaaataaaa attcggtatc
     4861 aaaaaactgt taccgataac atttttgaaa gcgttgtctt ttgcttctct atcacttata
     4921 gtgatgacgt catattaaaa cacaaggagg aaatcaatgg atacagctgc aatttatcat
     4981 cgaccggaaa gtgaatatgc atttctatat acgaatacaa gatttcgtat tcggcttcgt
     5041 actaggaaaa atgatatcaa aaaagtatat gtgctatgtg gagatccata taccataaca
     5101 actgaaaaat ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac caatgttcat
     5161 gattattggg aaattgaagt gacgagcgaa acaagtcgct tacagtatgc gttccatgtt
     5221 gttggggaag ataacacgga ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa
     5281 aatgtgatta ctgaaccaaa tttttatttt cgaatacctt attttcatca aatcgatcga
     5341 ttcactattc cagaatgggt aaaagaaaca gtatggtatc aaatttttcc cgaaagattt
     5401 gcaaacgggg acaaagcaaa tgaccccgaa cacactttac cttggggaag taaagatcca
     5461 gaccgagaag actttttcgg gggagatttg caaggtgtga tggatcattt ggattacttg
     5521 acggaccttg gcatcaatgg catctatttt tgtcccatat ttaaagccac atccaatcat
     5581 aaatacgata cgattgatta ttatgaggtg gatcccgctt ttggcgataa aaaactttta
     5641 aaaaacttga ttgatgaagc acataaaaga gggatacgta tcatgttcga tgctgtattc
     5701 aatcatatgg gtgtccattc accacagtgg caagacgttc tcaaaaacgg agaaaaatcg
     5761 atctataaag actggttcca catccgtttt ttcccagtag attcttatca gatgactgac
     5821 ttaccagaaa ctgctgaaaa tatcccatat gatacttttg cttttactcc attcatgccg
     5881 aaactaaata cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc aacttattgg
     5941 ataaaagaat tcgatattga tggatggcgt cttgatgttg ctaatgaagt cgatcatcat
     6001 ttttggaaaa aattcagaga agcagttaca gaaatcaaac cagacatcta tatccttgga
     6061 gagatttggc attcctctca agcttggctg caaggggatg aattccatgc agtgatgaat
     6121 tatgcattta cagattcgat taaagattat tttgcaaaaa agaaaatcac agcttcacaa
     6181 atggtcagtg gaatgaatca tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca
     6241 ttcaacttat tagattctca tgatactgcc aggatcttga ccctttgcca aggtaacaaa
     6301 gaattgatga agtctgtgat ggcctttatg tttcttcaaa aagggtctcc ttgtatctat
     6361 tacgggacag aaatagggat gactggtgaa gatgacccgg attgccgcaa atgtatgata
     6421 tgggagaagg aggaacaaga tctagagcta ttcggtttta taaaagaact tgtttctcta
     6481 aggaaacagt tgtctaagat tatttctgaa ggttctactc agtggctaat agtaaatgat
     6541 cgtgaagata agctctattt cacacggaaa ctagagggac agatcatcta tgtttacttt
     6601 aatcagtcaa aagaaccttg ggtagtagaa caggaaaatg aggtgattct tagtcaaaat
     6661 tgtcagttgt tagaagacgg gaaagcagag atacgacaat acggattttt gatcatgtgt
     6721 tgaaaactgt taccggtaac aacaagaaag cgtttgcaaa tgaagatttg tgcccattat
     6781 aataagggtg taacagcacg ttgaatacaa aggaggaaat gaagatgaaa aaaagaagtg
     6841 tgaaacagtt gggtttaggg ttaatctctt taggaatctt aggcggatta gcggcttgtg
     6901 gcaatggtaa ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta acagtttccg
     6961 ttgattctgg atataaagat tatataaacg aaataaaaaa cactttcgaa aaagaaaacg
     7021 atgtgaaaat caagctagta gaaaaggaca tgttcgatca gttggaatca ttagcgttag
     7081 atgggcctgc tggaaaggga cctgacgtta tgatggcagc atatgaccgg atcggtgcgt
     7141 taggacagca aggacatctg gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa
     7201 cagacaaagc ccaagtgact tatgatggaa aaatctacgg agaacctgca gtaatcgaga
     7261 cccttgtttt gtactataac aaagatttag tcgatacagc accatcaacg tttaaagatt
     7321 tggagaatct atccaaagac agtcgttttg cttttgagtc agaagcagga aaaaatacag
     7381 gatttttagc taaatggact gacttttatt attcctatgg tttaattgca ggttacggtg
     7441 gctatgtatt tggtgatgat ggcacaaatc cttcagatat cggtttgaat aatgcaggtg
     7501 ctgtagaagg aatctcttat gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc
     7561 aagacataaa aagtgcagga gacttcgcca gccagcaatt catgtccaat aaaactgcag
     7621 caatcattga cggtccatgg caagcgcaaa catacaaaga aaacaatatc aattatggtg
     7681 tagccaaaat tcctactcta aataatggac agccttatca accgtttggc ggaggaaaag
     7741 gatgggtagt cagcaattat gctaaaaaca aagacctctc tcaaaaatgg cttgattatg
     7801 taacgaatca ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc
     7861 aacaagctag ggaaacagca aaggctaaaa acgatgaatt aacaacagct gtgattgaac
     7921 aatatgaaac agctcaagcg atgccaaaca tcccagaaat gggcgaagtt tggacaggtg
     7981 cagaaaactt gatgttcgat gcagcatcag gttcaaaaac accaaaagaa tccgcagatg
     8041 aagcagtaaa aacaatcagt gaagccatag aacaaaaata ctgattaaaa tgataaccgt
     8101 ccatttttat aaaaaatgga acaggtgctt cagataaaag tggcaagagc gagatggaaa
     8161 attatcttgc tcttgttgcc attatcttaa aagcagtttg cggtttagca agtttagagg
     8221 gggcattcag atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg
     8281 ttcacttatt ccagggatgg ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt
     8341 tttcgccata ttcgtcctat tcattggtca gatgcttctg ggcggatttc atgcattggt
     8401 aggattggtt actttgggaa gtataccaat ggaagatcat tcattattta ttttgattga
     8461 aggcactttg caattcatca ttacaatcat tttcttgatt ttttacggtt tgaatctttt
     8521 agacgcttat cgagtagcga agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa
     8581 ggaagtcata cataatatag gtgatgaagg ttttccttat ctattgacac ttcccgctta
     8641 tcttctcatg atttttacga tcattttccc agtcctagtt acattgttca ctgcatttac
     8701 taattatgac ttcaatcata ttccaccagc cagtttaatt gattgggtag gggctaaaaa
     8761 cttttttagt atcttctttt taagctcata tcgtaatact ttttttgcag tcttttcgtg
     8821 gaccgtgatt tggactttat gtgcaacaac tttgcaaatc acccttggta ttctcacagc
     8881 ggttgttgct caccagaaat ttattaaatt caaacgtatt ttcggtatca ttttcttatt
     8941 accatgggcg gtcccagctt ttattactat catgagtttt tcgaatatat tcaacgatag
     9001 cgtaggtgcc atcaatactc aagtgattcc tttcataaac cagcttcctt ttgtagatat
     9061 cagtgcaatc tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta tcttagtgca
     9121 gggctggtta ggatttccct atatctatgt gatggttaca ggaatcttac aatcgatttc
     9181 agaagactta tatgaagcag caaaaatcga cggagcaaat gcatggcagc gcttttcaaa
     9241 catcacgctt cctgctgtct tcctagtggc agcgccaaca tttattacac agtatacggg
     9301 gaatttcaat aatttctcca tgatctatct gtttaacaat ggcggtcccg gaagcgtagg
     9361 cggaggagct ggttctactg atattcttat ttcctggatc tataaactta cgactggaac
     9421 ttctccgcag tattctatgg cttcagcaat cacgttgatt atctccgtca tcgttatctg
     9481 tgtgtcattg ctggtattta aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa
     9541 aaatttcatt cgagtgctcg attccaaaaa cgactttctc acttttttac ttactttttt
     9601 cttttaatac tgtcagtcat tatcatctat ccgcttttga tcacagcaag tactgccttc
     9661 aagtcaggaa atattgcagc ttttagttta gatctttcaa gtgactggac tttaggaaac
     9721 tttactcggc tattcagtga aacactttac gggacttggt ataaaaatac attgatcatt
     9781 tctgtcctaa caatgatcgt acaagtgact gtagtaactt tggcgggcta tacttatagt
     9841 cgctatcgtt ttatcggccg caaatcaagt ttgatgttct ttttagtgat tcagatggta
     9901 ccgactatgg cagcattgac tgccttttat gtcatggctt tcttattaga agcgctcgat
     9961 aagtactggt tcttgacaat gatctatata ggcggtggaa ttccaatgaa tacttggctg
    10021 atgaaaggat actttgacac tgtaccaata gatttagatg aatctgctaa acttgatggg
    10081 gcagggcatt tcaggatttt tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc
    10141 gttcaagcat tatgggcttt tatgggacct tttggggatt tcatgttagc taagttcctg
    10201 cttcgaacac ctgaaaatca gactgttgct gtcggcttac aaacatttat ttctgatgtg
    10261 acaaaccaaa gagtctctct ttttgctgct ggggcaattt tgattgcact accgatttgt
    10321 ctattattct tctttttaca aaagaacttt gttaccggcc tgacatctgg cggaacaaaa
    10381 ggataggagc agagtatcat gcaaacattt ccgttaaact atttttccgc attacgtacg
    10441 ccaactcaag tgtttgcggg acgcaaacta ttatcatggc ctaaattttt cttgatattt
    10501 gtatttttag tttccttgat ggtcatgcct gtgactcttt tttatgccaa tcagatccaa
    10561 gcgattccat tagaacaatt tctatccgtt cattcgctga tcgatgaaca aggaacccaa
    10621 aaattttctg aacttgaatt atctgaaact gggttacaag catcgcaaca aacgatagct
    10681 gtgacccctg aaattttggt aggtgtgtct ttatctgaga aacaacaatc cgatcatgga
    10741 acttttattg attttgagaa ggaacaatgg gtgatccaac aaaaggataa atctggtata
    10801 cgccgctata cgatgaatta tagcccatct ttccaaccag attctgtacg tacgcccgaa
    10861 gatttccaac gatttttaga aagagaattt tatgccagta atcgtccaac aatcatttta
    10921 tcttatagtt taagtcttgg tcttgtccta tttgtcatga cgagtctcat tttattcggg
    10981 gcttcctttt tcttgtggat gacaagaaaa agccaacttt catccattca tacattcaaa
    11041 gagagtgcaa atttgatgct gaatgttatg ggaataggtt ccatggcagc agcagttgta
    11101 gggtttatcc actttgactt tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg
    11161 ctgcttttat gggtttttgt caaaacaaga ttcaaagata agcgtgtcga atgacaagtt
    11221 taagcgattt atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa
    11281 tttctgaaaa aattaaactt cttagttgat tcttttcttt tttagtatta acgtaatagg
    11341 gtagtttttt taaggaggaa aatcatgtta gagaaaactt tatacaatca attattttca
    11401 cgttctttct cgctccctgt cgaagtaaat tattgggacg gtacaacgaa acaatacgga
    11461 gatacggaca ctcctcccca aatcaaaatc aatatccatg aagaaatccc aatgaaagag
    11521 attacaagta acgcttcttt ggcactagga gaagcctata tggatcatcg aatcgagatc
    11581 gagggaagta tccaagcatt gatcaacgat gcttatcagc aagcaaacag ctttttacga
    11641 ggaaaagatt atctaaaatg gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa
    11701 gaagatatcc atgcgcatta tgacttaggt aacgactttt ataaactttg gctagatcca
    11761 actttgactt attcttgtgc ttatttcaat acacctgaag atacgttgga acaagcacaa
    11821 atcaataaag tccatcatat cttagacaaa ctctttatca aagaaggcga aacgttgctt
    11881 gatatcggtt gtggctgggg aacattgatg ttcactgcta ccaaagagta taatgtcaag
    11941 gcaactggga tcacactaag tgaagaacaa tacgatttga ttaccaaaag aataaaagaa
    12001 gaacatttag aagataaatg ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa
    12061 acgtttgatc atatcacaag tgtaggtatg ttcgaacatg taggttctga aaatcttgaa
    12121 ggttatttca aagtggtaaa agatttattg aaaccaaaag gaacagcttt gatccatgga
    12181 atcagccgac aacaaggcgg tgcgacaaat gcttggatca ataaatatat tttccctggc
    12241 ggttatgtcc ctggtgttac tgaattagtt gataatatta caaaaaacga tttgcaactg
    12301 attgatttgg aaagcttacg tcgtgactac caattgacat tagaacattg gacaaagaat
    12361 tttcatcaag tccaagatca agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg
    12421 gatctatatt tacaagcttg tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa
    12481 tatttgcttg ttcaacctgg aaacaatgac atcccaatgc atcgtgcata attgaggcaa
    12541 aagtactgtt taaagactat ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac
    12601 ttttttgtct aacttctagg attgtctcta aagcttagtt tctttttctt tatattttca
    12661 taataaaagt ttggttcttt aatttttaaa catgacagaa aattttttct tcgttatact
    12721 tgtaatagaa tactatttta taacaaaggg gatcgaatca atgaaaaaaa ctgttattta
    12781 tactagttta tttgcagctt tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac
    12841 atctgggaat acgtcttcca gtagcgaaca gcgctcttca caggtatcgg actcctcaac
    12901 aaaagcaagc acatcttcgt ctagccagac aaaaagttct agtgaagcaa gtactcagtc
    12961 agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc caagataaat acccagatac
    13021 tgcaattact agtttggaat tagatagtga ttggggatct tacttctaca agatagaagg
    13081 tgtcgatgat caaaaagaat acgaagcaag aattgatgct caaaatgaaa aaatggagac
    13141 agagaatcca gaaactctag atcgagatga acaaaatggt caaaagaaaa aagaagatgg
    13201 tctggatgtt gataaattga tcacgattga ggaagcagca aaaatcgccg tcgacaaagt
    13261 aggaagcggt acggcaacag attgggattt ggataaagat ttaggtacta cttattggga
    13321 agtcaaagta aaagacggca atcaaagtac atcagtcaag atcaatagcc aaacaggcga
    13381 agtactgtca actgaacaag atgattaaga atttcttcgg aaaacaagag ttgatttttc
    13441 ttttagtcat gatacactag acaaaataac ttgtttgaag gagcgattta cttgagttta
    13501 gaaacgacct ttgccaatca tatctttgcc aatcctttga tgaatgcatc tggtgtccac
    13561 tgcatgacta cccaggaatt agatgaactg gctcattcag aagcaggagc atttatcact
    13621 aagagctgta cgatcaatga acgaaaagga aatcccgaac cacgctattt tgatgttcct
    13681 ttagggagta tcaattctat gggacttcca aacttaggtt tttcttatta tctagagtat
    13741 gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac cgttattttt ttccattgcc
    13801 gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat
    13861 gggatcactg aattaaattt atcttgtccc aatgtgcctg gaaaaccaca actagcttac
    13921 gattttgagg ccacttatga aacactgaaa gaagtattca gcatcttttc taagccgcta
    13981 ggaatcaaat tgcctcctta ttttgatttt gctcattttg atcagatggc tgatatcttg
    14041 aatcagtttc cattgaccta tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc
    14101 gatacagaac aagaagcagt agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga
    14161 gagtatatca agccgactgc tttggcgaat gttcgcgctt tttatacacg tttgaaacca
    14221 gaaatccaaa tcattggtac aggtgggatt cggacaggac aagatgcatt tgaacatctg
    14281 ctatgtggcg caagcatgtt acagattggt accgagcttc ataaagaagg gccggaaatt
    14341 ttttctagaa tcataaaaga attaacgcag attatgtcag aaaaaggata tacgtcaatt
    14401 gacgaattta aaggcaaatt acgcaccatt tcatagcaaa gagacgaagg tcatgattga
    14461 tcttcgtctt tttttataaa aaacactcgt ctgttcattc ctgtgcaagc gtactcattg
    14521 tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa ggaggataat atgaatgatg
    14581 caacagaatt agcaaaagag cttaaagaaa aagaatttag ttttcaagaa ctagttacgg
    14641 atatacagaa aaaaataaag aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac
    14701 cattcgagag taattcccac gagctattga ttccccagtc agaaaaaggg ccattggctg
    14761 gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta gctacttcag
    14821 gatctcgttt atttgaaaca caccgagctt ctcatacatc taattatgtg cggcaagcag
    14881 aagcgatagg tctagttcct tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata
    14941 ttacagaccc agtcatctat ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg
    15001 gcggatcaag tggaggagct gctgctgcag tttcatcagg aatcgttcct ttagctggtg
    15061 ctagtgatgg cggtggttct attagaatac ccgcttcgtt ttgcggattg attgggctga
    15121 aaccttcgcg agggacgatg ccagttggtc catatgcttg gagaggatgg caaggagctg
    15181 ctattgattt cggcttgaca gtttctatga gagacacgga ggctttattt tatgggatga
    15241 gaagtatcca ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa acacatccaa
    15301 gaaaaaaacg gttgaagata gctttttgtg ttgattcacc gatccactca cagattagtg
    15361 aagaggcagt acttgctgta aaaaaagctg tagatttcct tggaaaagaa gggcatgatt
    15421 tgacagagat tccttaccca ctagatggtc gtaaactgat cacgtcttat tatcagatga
    15481 atggcgcaga aacagcagca atgatccgct cgattgaaac aggcatccag cgagctgtca
    15541 gaaaagaaga acttgaaccc atttcttgga ctttgttcca atatggtcag aagctctctg
    15601 ccgctactta catcgattct ctgcatgtat gggatgaagc agcaattgtc atggaaaacc
    15661 tacatcaaga atacgatcta ttcctttcac caacgactgc atttaccgcc cctaaaatca
    15721 gcgaagattt gcaaagtgat gcgataagag cacagatgga acacatcagt gattgttctg
    15781 aagtagaagg tttagacgtt gtttcttcca tgtttgagga gagcttgaag atcacacctt
    15841 atacacagtt ggctaatctg actggacaac cagcaatcag cttgcctact catttgacaa
    15901 aagaaaacat gcctctagga atccaattta tggctgccag aggaagagaa gatttgctat
    15961 ttcaaatcgg ctatctattt gaagcaaatc atcagttcca tttaccaaat ccatggaaat
    16021 agcagaaaca aggagaagaa gaatgaagaa aaaaagattg tttgctctaa taactagtgc
    16081 cttttttatt ttaagcgctt gcggtacagc tgccgctaat caggaagata acagctggag
    16141 taatgtagaa aaaaagaaag aattagtagt agcgacttct ggcacactgt ttcccgcatc
    16201 ttattataat gatcaaaatg aattagttgg ttatgatgta gatttggcaa aagaggtggc
    16261 taaacggtta aatgtcaaaa tctcctttaa agaatacaac gtggatggac aaatcacttc
    16321 tgtttcaaaa agagaatcag attttgctgc gaacgatttt ggtatcacaa aagaaagaga
    16381 aaaaaaattc tccctttcag agactttcaa atattcattt gacagtatga tcgtacgaaa
    16441 aaaagatgat tcaggaatcc actcacttga agatcttaaa gggaaaaaag cagctggcga
    16501 acccaatact agctatatgc gtttagcgga aaaacacgga gctaaattag tcacatatga
    16561 caatgccacg aatgatcagt atttaagcga tgtagcaaat ggtcgaacag atgttattct
    16621 aaatgattat tatctgcaga aaatggccgt atctgcttta ccagatatcc ctgtaaaaat
    16681 actggagaac gtttatttca atgaaaacga aagtggtttt ttattgaaag aaggaaatga
    16741 tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg aaagaagatg ggacgacaaa
    16801 gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc
    16861 tgagtcggtg agtgagtgat gtactttcct ctaattgatt tttccctcat gtggacttca
    16921 cttcctttcg tactgcaagg cttggtatac acattaggga ttggcttcgt gtcgtttctt
    16981 ttaggaaatc tggtagggtt gcttttgaca gttttaggtc ttttggattg gctgcctctg
    17041 aatgtattta ttcgttttta tttatcgttc tttagaggca ttcccgcact agtcctgctg
    17101 tttctgttat attttgggtt accttatcaa ttaagtgcct taacggcttc agtcatctgc
    17161 tttacgatta ccagcagtgc atttattggt gaaatctatc gaggatcact agcaggcgtt
    17221 tcttcaggtc agtgggacgc tggtttagct tctggttttt ctttctttgg ggtattgaaa
    17281 tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg
    17341 gatctagtga aaggtacttc gttagcagca acaatcacag tgccagaaat atttcaaaaa
    17401 gcaaagatca taggcggtcg gacattcgat tatttttctc tctatatttt agttgcactg
    17461 atttattggg tcatttgtat cgggatcgaa aaagtgcaac agtatttaga aaaacgttct
    17521 atgcatcagt caatctgaaa aatagaaatg gtgctagcgt ggctggcacc atttctattt
    17581 tttgtttctt gtatattctg cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga
    17641 aaatatgttc ggaggtagaa aacatgaaga ccccgctaac catcgaaata gaatcctcgt
    17701 tataccaata ttgcatcgag caaggcggct tggttgtaga agaagtcacg atgcctgatg
    17761 accaaggaat tgtagatacg cttagctgct tgacaaaatt tgatggaagc cgagaatggc
    17821 gatgctatga gctgaaagtg acaaaagcag attttcgctc tacagccaaa ctgtcatttg
    17881 ttggacatta taattatttt gttttaccaa gtaaactata tgaagaagta aaaactgaaa
    17941 tccctacagg aataggtgtt ttagtgtatc gtccgtacac ggttgaagag gagatgcctg
    18001 cctctggtac atttctgatt gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag
    18061 aagctttgac aaaccgcttc atggcttctt tattcaggga agtaagaaaa gcaaagcaaa
    18121 tggagcgagg cgtaaaatat tttccaacag atcaattgta caaagaattg aaaagacgtt
    18181 cagaaaatca agatccattt tctaacgaac gatattatga acggttttta gaggaaatag
    18241 ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc gcttcagcat gattatgaat
    18301 atttgaggca acaacagcag atccgtcggt tgcctacaga gccattggag tgaaaaatat
    18361 gtactatcct tatttaagag gaaaacaatt tgacctatta gcattaaaag aagcactgtc
    18421 gcgaggattg ctctccaata aaatccaacc agtgatcgaa ccagtacgtg attccgctac
    18481 gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat ccaatcgctg tgatccaaaa
    18541 tccccaagta ggacaattca aattattcga tcagcatgtg cacagttgga cagtcaaaga
    18601 aaatagctct gtcgtccctg cacagatctt aacgccagaa aatctcagtg aagtactgga
    18661 ttcacctcca gcgtttcttg ttttcgatgg acagcactac ccaaaagata cagaggtgtg
    18721 gaaacagttg gcaggagttg attcgaaatt tttgatacca gatactagtc gctttcgcat
    18781 ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa agcatgttga
    18841 aagttatgca gataaaacag atgacttttt tagtgatgac tatctttttt tccacgcaga
    18901 cggatatatt ggattttctg atttcacgat cgaaggtagc cgttatttcg ataaagggtt
    18961 ccctagccga gcgcttgcta tccatctaac gtatattgat gcttacggca atatacgcgt
    19021 gaaacatttt gtctctgaca gtaacgactc tgctaaagac caagcattga agtttcttga
    19081 agcaggagat aaaatgaaaa aatggatcat gcgtcaccat catcaactct tgattacttc
    19141 aggaatgatt gaattattgg ctctttacca gcaacaaaaa ttccctggac tgggagtgtt
    19201 gaaaaagtgg tcacttatgc atcatttgga acttattagt cacctgctag atcatccaaa
    19261 agattggctt aaatattatt caaaagttca ggaactatat gaagtgatcc aatgattaga
    19321 taatgtaaat gaaaaaggaa tagaaaagag gaaactggat gtatattcgg caaacaagga
    19381 aagaagaagt agaagaagtg gtagaaatca ttaatgccgc agcggatttc attgcttcgc
    19441 aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt ttgaacgata
    19501 ttgaaagaaa agaaagctat gtattaatag tggaagatca aattgctgga acagctgcat
    19561 tagtctcaag cgtggatccc gtttacacaa aaatagatgg tagttggaaa gaatcaactg
    19621 gtccttattt atcgattcat cgtgtcgctt tagcagaaag tactcgagga aaaaaactag
    19681 caaaaccgtt cttagaacat ttgttgacga ttgcagccag caaaaatatc caagatatcc
    19741 gtatcgatac ttatccagct aatgaaccga tggaaaagac catctattct gctggattca
    19801 cttatcaagg gatgattgaa tttccttttg aacacggcga aagaaaagca tatcaaataa
    19861 tcatcaattg actataaaat catctgagca aagataaaga cattaacact actgaaaaaa
    19921 acaatcatat gatagccaag aggttgtaac agaagtggcc agcttcaaaa aacaagaaag
    19981 aatttccgaa aatgcttctc aaatttttgt ggaattttag cgtattttac gaaatagctg
    20041 ctttctgaaa ctcccttcat cgagaggtcg tgacatagct tttgtcacga cctctcggct
    20101 tatcctacga gtattattcg atacatttat atcaataatc ctttttttat tttatctgca
    20161 gcttcttgga acgctccatc gtttgacacg actaaaagtg aggggatagc tttcaaacgt
    20221 ttcaactgtg ctaaatcttc tacataaacc gccagtctgg ctgaaatctg ttcagatgaa
    20281 tctccacgtt ttttcaggcg cgattgcaat gtttctttag agatcgtaat aaagatagga
    20341 cggactaccg tcccgaaata ctcatataaa tgccaaaaac catttgctgt caaaacggcg
    20401 taacaatttc ctactgtcag tttcttttta atttctgctt tactggaacc atatttatgt
    20461 tgagcatatt ggtcccactc tgcaaactca tttcccttga tcatctgatt gaatgtattg
    20521 tcagaaacaa aataataatc ctcttgatct ttttccccag gtcttggtga tcgattcgtg
    20581 taagtaatga tcttttgttc cggctgaaaa atagcttctg cgagtgaagt ttttccagaa
    20641 ccagaaggac cgataaatac atagcaataa ttcattgtca tttctccttt gatgattgaa
    20701 caagtgaaaa agctttggaa aatatttcca aagcttttaa aagtaacttt gcttttttgt
    20761 atattatacc agcaagccag tcacaatagc acttaaaaag ctgactaaag tcgctgttag
    20821 caaaattttc aatccgtgtt ttgcgacaag atttcctttt tcttcattta atcccttcat
    20881 cgcaccagaa atgatcccga tagaagagaa gttggcaaat gaaacgagga agacagatat
    20941 aattgctgtt gtacgctcag taaaatggaa attaccattt gctaattctg tcatagcaac
    21001 aaattcattt gtaaccagtt tagtcgccat gatactaccg gcatccactg cttctttcca
    21061 tgggattccg ctgatgaagg caagaggggc aaagatgtaa cctaggatct cttggaaagt
    21121 gatgccgaat atcccgtgga agatagcatt gatcatggca atcaaagcaa caaaaccgat
    21181 aagcatagca gcaaccgtga tcgccacatg gaaaccatca agaatgtatt ctcccaacat
    21241 ttggaaaaat gtctgttctt tgttttcttc aatgatcagc tcatcttctt tttcttctaa
    21301 tacataggga tttacaattg aagccagaat ataaccgcca aataagttca aaacaagggc
    21361 agtgatcaca tatttagaat cgatcagtgc catataagat ccgacgatcg acatagaaac
    21421 tgtagacata gccgatacac tcatggtgaa caaacgtttc tcagatagga agggtaattc
    21481 cttttttatg gaaataaaaa cttccgattg tcctaaaata gcagaagcta ctccattata
    21541 agattccaat ttgcccatcc cgttgatttt acttaatcct agaccaacag ctttcataaa
    21601 taatggaaga atcttgatat agcgtagaat accgataata gcagaaataa atacaattgg
    21661 catcaatacg gaaagaaaga aaggtgcact tcctttattt gctacaccgg caaaaacaaa
    21721 gtcaacacct tgcccagcaa aagcaagaag atgatcaaac atttttgcca atacagaaac
    21781 aaccgctgta ccaaaagttg tacgtaataa tacgaaacca agaacaagct gtaaaacgaa
    21841 catcacaatt agtggtttca atttgattgc ttttttgtta ctgctgatca aaaaagcaag
    21901 ccctaatgtg acaaataaac caataatacc taataaataa ctcaaataat cactcctatt
    21961 ccaaattccg ttttatttta cctgttttgc aaataaaata ctaatcttta gtgaaatttt
    22021 cattactttt ttagagtgaa acgcattcat tttccattag aaaataaagc gaacaaaata
    22081 aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac tatcgattaa
    22141 gctaatagtt tcgttttcaa catttataag taaacttttt ctattggtaa tatagatttt
    22201 cggaagggta gacaggtgcg caggtaatat cgattcctag ttttttcaat gtttgttcat
    22261 catttttatt gaccatcaca gtagagtggg cttgtgctcc ctcaagttca gctagtttat
    22321 cataagcaac ttgagcgatt ggattcgtta ctgcactgat tgataaagcg atcaagactt
    22381 cattgatgct tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat ccttgtattg
    22441 tttcaagaac taatggtgac aataaaggaa tgtcatctgc gatatgggct aaatgtttta
    22501 ctgcatttaa gataactgcc gctgacgcat ccatcaatga acttgtacgc ccggtaatga
    22561 tgacttcatc tggtaattcg attgcaatca cagcagaagg atcttttgta ggactttgcg
    22621 cttgaatctt atctgcatag tcatgggcag ggactaccac gtttcggtct tcaggacgta
    22681 aaccgacttc ttccataatc agtttgattc gattaactgc ttcttcgtcg atcaatcctt
    22741 ttttgaagtc gcattctgtt tggaaataac gacggatgat ttcttgttta gaggcttctt
    22801 ggacaacggt atcatccacg atcccaaatc ctacacgatt cacacccatg tcagtaggag
    22861 attgataaac agattcttta ccagtaattt tttcaataat acgtttgatt acaggaaatg
    22921 tctcaacatc ccgattatag ttcacagcta ctttattgta ggcatcaaag tggaaggaat
    22981 cgatcatatt gacatctttt agatcgactg tggctgcttc ataagcaatg ttcagcggat
    23041 gtttcaacgg aacattccat actgggaaag tttcgaactt ggagtaacca gctgcatgtc
    23101 ctttttggct ttcatgatac agttggttca aggaagtagc gagctttcca cttcctgggc
    23161 caggagctgt cactacaacg attggtttag ttgtttcaat atactcattt tttccaaaac
    23221 catcttctga gacgattttt tctacgttga ctggatagtc ttcgatttct gagtgtttat
    23281 agaccttgat gtttcgtctt tctagtttat tgataaagac tttcgtggca ggctgtccac
    23341 tatagcgagt gataacgaca ctgttgatta ataagccata accacgtaat tcatcgatta
    23401 accgcaagat atccatatca taagtaatcc cataatcacc acggatctta ttacgttcaa
    23461 tatctccagc atagacgcaa atcagtattt cagcttgatc tttcaatttt tgcagcaatt
    23521 tgattttcgc atcttcttca aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta
    23581 gcttcccacc aaattctaaa tataatttgt catagtggtt cactcgttcc aaaatatacg
    23641 ctgactgctc ttcaatatat ttttctgaat caaaaccaat ttttttcact aggttccctc
    23701 cttaagtact aaagcatttt ttcatttttt tgaggattcg tcaactggaa gagcgaaatt
    23761 cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct agatagttat tcattggtat
    23821 gatagttttt gataaggaag gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat
    23881 aaacactata tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg
    23941 gaacaattga cgtttactta tctaaacgaa gaaacgcagc taactcgtga tgagcatcag
    24001 cgcattcatt tcacaacaag agatctgcct agtaaataaa aaataagata aaggtcatcg
    24061 gattcgattc tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa
    24121 ttttggtgca gcctgtttta tggagcattg cccgctcttt tttataacaa ttttttacag
    24181 aaaatggtac tattgaagta ttaataagtg aaaaacagat gagtaagagg aggcaagtag
    24241 tatgaaagag ccagatacaa atgcaaatta tgagccagac gaactagagg agacgaatgc
    24301 ggatcaggat acaggacaag aatcgtcttc tttttacgat atcaaagaaa aagcaagcat
    24361 gaatctttcc caattattag aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga
    24421 tatccctgag atctaccgtt tatacaatgg ccgattgaat gaagaattaa aaagaagctc
    24481 aaatgaaaat cacgagatcg atcaattgct gatgcgtaag atccatgatc gttttttaca
    24541 ggaatttcct tttatggaac aagtagaaaa catctcgcca acgatgagct attacaaaat
    24601 cggcacttat taccatgatc ggcccacggt cggtattgat gcaagtctgc cagaaatttt
    24661 tgttttgcct aagatcgatg aagagtggga gaaatattcc caaccggagt caacggacta
    24721 tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact gctcagacag aaattgaacg
    24781 tttagatgaa caaataaaag aaatcaatcg acaaatgact gatttgaacg acaataaagg
    24841 attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga agaagcaagt
    24901 actatctaat gaaaaattag gctggcttcc ttatattgag acgcctgaaa caatccagca
    24961 gcaaaaagaa gcattgaagc aagaagcaag agcagaccag cttcgtgcag ccatcgtaga
    25021 aaaagaacaa aggcaaatca gacgctattt cggtagcaaa gagggattcg gtcaagcaat
    25081 ccatgaattc ttgatgaatt atcttggcaa tgagaatcct aataatcaat ctaatgaagg
    25141 aggttccgag tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa gaacaaacag
    25201 aagatcatga agaaacgaac acttcattgg aagaacaaac aagtaatcct ctggatgaca
    25261 cagaagaaat ccctatgcta gaggaaacaa aaatacacga aaaagaaaaa gtaactccga
    25321 cgaatacgaa tactgcagaa aacacccatg cttacgaaat aactgaaaat cccgggtatt
    25381 tagaaggaaa agagcaagat catcaagata tcgtcagttt gattgaaaca gcggaagcag
    25441 aattagcgga tttacgtatg agaaaagatt tgatcgaatc agattttcca caattattag
    25501 aatcatatga tcgttttata ttgaatgaag aaccaatctc cacgatcgga aagacaagcc
    25561 tgatcgaata cttcacgtat gaattactga aaccgttgaa tgatattggt ctggaacggt
    25621 caaattcagc ttatgacaca tggaaaacaa gacatttttc gatcaattat acgttgaatg
    25681 gacaaaacga attagtcttt tcctttgtat taccgaccat cgatcaacga taccaagtgg
    25741 aatcgatgag tctcctaaac gtatcaccag agacaatgga aatcaatgtc caagatgacc
    25801 gggtgctttc tttgatccga cactggtcag tggatcgagt attctctgca gggcagatca
    25861 cgatattcaa tcacaaattg aatcaattat tagcccatgc aagaacactt ggttttaccg
    25921 ttaatcagac attactagac aatacgaagc cacttcgttt gaatcttcag agcgaatttg
    25981 aattgacgaa tgaagtattg gatgatattt ttatcgtggc aatgaaaaat ccatcctacg
    26041 atatggaaaa gatcaacgaa actacttatc aagtacgatt agataaagga cagtctttaa
    26101 cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga gactacccac
    26161 gatctgtcat tgatttcttt atcaattacg aattcttggt cccattaatg gtgagaaagt
    26221 cctgattgga taagttggat taccagatgt cattcagata attctgttcc aaaggacttg
    26281 cggcagttat ccagaataat tgtcacaagt cttttctgta tgtactaaat ggacgtgaat
    26341 aacttgctga tgaaggaggg atttcattag gaatgtccac aaataaaata gcacagctta
    26401 gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt tcaatttatt ccgaatttcc
    26461 agccaatgac tgctattttt ttatttatag tactttactt aagcttaaaa gatgcgttaa
    26521 ttgtcatgag tctttctatt gcgatcagca gtttttattt tggtgttgga ccatgggtca
    26581 tcggccaatg gatcagctat actgttgttc tgagtttgtt ttcaatcgtc tgcttgagaa
    26641 aaacagtgca aaggaatcta tggttaaagg gagtctgttt ttttctggcg ggcatggttt
    26701 acggaatagg tatgaccgtt tttgatacgc tgttatatcg tttgcctcaa ccttggatct
    26761 attaccttca aggggtgtcg tttgatttga tgcacagtat aggcaatgtt gtgttctttc
    26821 ttgtttttct gccagtcgtc aaacgttttt acaatcattc aggaggaaaa aatgaaaaaa
    26881 ataatttata gtttgcttgc aatgagtgtc agtatactca ttttgacaag ttgcagtaca
    26941 acctatacac aatcggcttc caaccaaaag gaggaagtaa aaacatcgca aaaagcaaca
    27001 atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag aagtggtctt caaaaaaggc
    27061 gatgatttac tgagtatttt gaaacgaaat tttgaagtca aagaagataa cggcttcatc
    27121 acttcgcttg aagggcactc acaggatgag aaaaatcaac tctattggat gtttactgtg
    27181 gacgggaaaa gtgctactac tggtgccaaa gagatcaaat tgaatgatgg acaatcggtc
    27241 attttcaatt tgtctaaact ataagtttca taaaaaactg ttaaagggcg aatattgcca
    27301 taattcagtg agttcagttg atgaaagaag gaaaataaca tggaaaattt taaaaaaagc
    27361 ggaatgtttt ccgtaattgc tgctctaggg gctaatattc tagttgctat cagcaaattt
    27421 gtcggctatg caatcagtgg cagtgccgcc atgctaaacg aaagcatcca cagtatcgtt
    27481 gattgcagca atcagatttt gctgttattc ggtgataaac aagccaataa aggacagagc
    27541 gagttgcatc aatttggtga aggacgagca aaatactttt tcagtacaat cgtcgcaatg
    27601 atgcttttct tcggcggtgg ggcacttggg gtgatggaag cttttgaaaa gttgacgcat
    27661 ccatcccatg aagtgggtaa tccgatcatt gttatagcaa tactgttatt tggtttggta
    27721 atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca aagaattgaa tactgaaaaa
    27781 ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg aaatactgat tatttttacg
    27841 gaagacttgt gtgctgtaat cggtttggtc ttggctttag caggaacaat attgacttat
    27901 gcaaccggga ttgctgcatt tgatgcgatc agcggattgc tgatcggttt cttgctgatg
    27961 ggagccgcca ttttcctagc aaaagaattt tatagtctga tcatcggtga aagcgtgaca
    28021 gcaagcgatt tatcaaaaat caagttagct tttgaacgtc ctgagatcag tcggctgatc
    28081 gatataaaaa cagttcattt aagtccaacg gagattttag ttgcagcaaa agcagatatc
    28141 gttggtaaca aagagaatga agcttactcg gtcatcaatg atattgaaaa aaatatgcgt
    28201 cagtctcttc ctgacaagaa gatgtatata tacattgaaa cagataaata tgacccaaat
    28261 tattctcgta aataaaacgt ttattcatga attgaagcaa aaatgagaaa aatataatct
    28321 tgtttttaaa taaaacggct acaatttagc gaatttatgt tacaatgagt ccgctgttta
    28381 tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga aaagtgcgcc atggcttcaa
    28441 gctttgcatg cagtgatgcc actttgtata agctatatac cagtaggatt agcttgcgga
    28501 gtattgttgc aaaaagttgg atttgatcct ctcttatcag gactgttatc gattctggtc
    28561 ttttccggtg gcgcgcagtt tttagttgca tctatgctta cgacgcaagc atcatttgca
    28621 acgacattat tgatggtttt ctttttagaa ttacgctata cattattagg agcaagtctt
    28681 gccggtttta tgaaaaaaga aaagcggacg tttttggctg tcttttctca atcactgaat
    28741 gatgaaaact atgcggtgaa ctacttaaag ttttcaactg acccttcctg gaataaaaga
    28801 aaggctttat atgtcaattg gttctcaatg actgcatggg ccggtagcaa tatgatcggt
    28861 aattttttcg gctcggtgat tcgagtagat gctgaccttg tccattttgc tttgacggcc
    28921 atgttcattt tcatgtttat catgcagatg aaaagcgcac tactgatctt tacaggttta
    28981 ttctcaggcg tattaggggt cgtattcatg gtattattcc aaaatacttt cggattgatt
    29041 gcagcaacag tcattgcttc atttgccgga tttatgctgg agaaggcatt caaaaaagaa
    29101 aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg agccgctctt caacttcccg
    29161 gaacaggagg ccaatcacca tgactaaact atatttgcta cttttagttg cttgtctgtt
    29221 catcgtggca tatatcccgc gcttgttccc gatgctttat tttactcatc gaaaagtgcc
    29281 atcctggttc agtgactgga tgaaatatgt accggtggca ttatttgcgg cgttagcttt
    29341 taaagatgtt ttcattactc atgaacatct ggatattgct tggaatatca aaatagctgc
    29401 aatgattcta gtagcaggcg tggcttacaa aacacgttcg atggcgttgt cagttttgac
    29461 aggacttgcg tctgtctttt tgctgtctat gctttaaact gttttatttt attaaaaaca
    29521 gagattgcga cataagttgt tcaaactgat ctatccgaac tgcatttgca acagtcaatt
    29581 ctggatgcaa gtttattcgt agggataaaa aagattagtg ttggcttatg atgcaagctc
    29641 ttttttattt taggaattag agagggattt gtccttctat gattttttgc tataataggg
    29701 aagatgttta ttttatgaaa gaatagttga gggaatttaa atgaataaaa aattgatgca
    29761 aggaacattt tggctaacct ttgctaatct tctatgtaaa gttttagggg tagtctattt
    29821 gattccctgg ctaagtatga tggggaacaa tcaagatggt atgcttgcta cgacattgta
    29881 taatgttggc tatcttcctt atggattgtt cctgatgctt ggtacagtcg gatttccaaa
    29941 tgcgattgca aaaaaagtag ctgtagccac aaaagaagga gataacgctg cttgtcggct
    30001 gatttttaga agtacgatca atatcatgtt tgtcatcggt atcgcctctg ctgcactgat
    30061 gtatttattt gctcctttac tagccggaat cagtccgatt gctaacgtta ataatgggat
    30121 actagctatc agaagtttat gcccatcttt gattgctatc ccaattctta gtgcaatgag
    30181 aggatatttc caaggaaaaa atgatctgcg gccttatgga acttctttga ttatcgaaca
    30241 ggctgtgcga gtgatcgtta tcttggctgg tacttattac ttgcgcgtat tgactgacgg
    30301 aaccatatta gaagctgtat tgatcagtac agtcgcttct ttcttcggtg ggttagctgc
    30361 gattatccat atgtatatcg tcggacgcag aaaagacttc ttcgaactta aagatttttt
    30421 gatttccagc cgttatttcg aaagagaaaa ccgatctgcc tctgtctcta tcattcggga
    30481 aacactaccg tttatttttg tcggctcagt cattactatc gttcaaatga tcgaccaagt
    30541 aacgatgaaa ccactgcttc actttttccg tccagagatc gcagatcagc aactggaatt
    30601 cttattcagc cgtgcttctg tcaatccaaa taaattgaca ttgatcctga tctccatggt
    30661 aggaacagta gctataagca gcttgccaat attaagcacg atgaagaaaa aagatcggat
    30721 acaaatcgaa aaaacagtag gagatagttt ttcgattgcg ttattgattc ttcttccttc
    30781 attgactgga atgtctttat tagctggacc actatacaca ttgttttttg gctatgatcc
    30841 tgaaagtgtc ggatatttcc aaatggcttt gctagcttct ttgtttttct ctttattcac
    30901 gatcctatct acgatgcttc aatctttgaa tcaccatcgt tttgcgatcc gattaactgt
    30961 agaagcaatt atcctgaaag tcatcttcca agtaatcgga ttaggattag ttggcggata
    31021 cgggatgagc ttatcaaatg gcttagcatt cggtattgtc tttgttcgcg gctatcactt
    31081 tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc agcaattttt tcttaaaaac
    31141 tttccgctcc accctgatta tgcttgcagt atgttttgcc gttttctttt tgctgaatca
    31201 gcgattgtca atggaatcaa aaagctatgc aatgatttat tgtgtggctg taggaagtat
    31261 tggcggctta gtatttttat tttctcaatt cggaaaaaat gggtttcgaa tgttgaaaaa
    31321 tgttaaacat catagcagaa gcaaagaata ggttaactaa tcgttttttg ctgtcacttg
    31381 aaaaacggca actcgtatga ttacgcttgt cgtttttcta ttttccaatt ttagtttcat
    31441 atctaatctt acttatgata tagttataag gtctggaaaa attctttgaa acgaggaaat
    31501 taaacacatg aaacttgcag tagttacaga tagttcggca tatctgccgg aacgtatcaa
    31561 gaaccatccc gacttattcg ttattccaat ccctttgatt atggatggga aaatttataa
    31621 cgaaggaatc gatatcgaag cggatgaata ttatggactt ttaaaaaaca gtaaagattt
    31681 tcctactact tcacaacctg tcgtagggga agttttgggt ttatatgagg aattgaaaga
    31741 gaaagggtat gacacgattt taagtatcca tctttcttca gggatttctg gctttgtcaa
    31801 cacattattt gcgatgaaag atgacatcag cggagtaacg gtcattcctt atgactctaa
    31861 gatcactagt atgccgatgg gacacatggt agaagctgcc ttagacttaa atgatgaagg
    31921 tcagtccctt gatgaaatac ttgcacatat cgatcgaatc cgagataata cctatgctta
    31981 tctgattgta gatgatttga acaatttagt acgaggagga cgtttgacaa atggtgcagc
    32041 gctgattggc agcttattaa aaatcaaacc gatcctgaca ttttctgaag ggaaaattgt
    32101 tttattcgaa aaaatccgtt caagtaaaaa agcatttgct cgagctgaag aaattattgg
    32161 tagaagaaat aacgaaatac aaccgccagt caaactttac gtgatacatg caaataattt
    32221 agaagtagcg attgcggaga aagagaaatt gcagaaaaaa tatccaaatg cagaaatcga
    32281 aattggtcat ttcggaccag tcattggtac acatttaggt gaaaaagcca tcggcattgc
    32341 catttcagcg caataattcc ttttattcaa aaaacatacc aaaaatat
//
LOCUS       DAYLZO010000015        32319 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.015, whole genome shotgun sequence.
ACCESSION   DAYLZO010000015 DAYLZO010000000
VERSION     DAYLZO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.015"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(22..216)
                     /gene="ssrS"
                     /locus_tag="ACGVJG_000696"
     ncRNA           complement(22..216)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGVJG_000696"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(288..1574)
                     /locus_tag="ACGVJG_000697"
     CDS             complement(288..1574)
                     /locus_tag="ACGVJG_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFJ6556675.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            1712..2383
                     /locus_tag="ACGVJG_000698"
     CDS             1712..2383
                     /locus_tag="ACGVJG_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFJ6556676.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            2349..2837
                     /locus_tag="ACGVJG_000699"
     CDS             2349..2837
                     /locus_tag="ACGVJG_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFJ6556677.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            2850..3797
                     /gene="prmA"
                     /locus_tag="ACGVJG_000700"
     CDS             2850..3797
                     /gene="prmA"
                     /locus_tag="ACGVJG_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFJ6556678.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            3798..4562
                     /locus_tag="ACGVJG_000701"
     CDS             3798..4562
                     /locus_tag="ACGVJG_000701"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFJ6556679.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            4795..7008
                     /locus_tag="ACGVJG_000702"
     CDS             4795..7008
                     /locus_tag="ACGVJG_000702"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFJ6556680.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            7019..7465
                     /gene="dtd"
                     /locus_tag="ACGVJG_000703"
     CDS             7019..7465
                     /gene="dtd"
                     /locus_tag="ACGVJG_000703"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFJ6556681.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            7636..8895
                     /locus_tag="ACGVJG_000704"
     CDS             7636..8895
                     /locus_tag="ACGVJG_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFJ6556682.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(8984..9931)
                     /locus_tag="ACGVJG_000705"
     CDS             complement(8984..9931)
                     /locus_tag="ACGVJG_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFJ6556683.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            10325..11617
                     /gene="hisS"
                     /locus_tag="ACGVJG_000706"
     CDS             10325..11617
                     /gene="hisS"
                     /locus_tag="ACGVJG_000706"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFJ6556684.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            11632..13401
                     /gene="aspS"
                     /locus_tag="ACGVJG_000707"
     CDS             11632..13401
                     /gene="aspS"
                     /locus_tag="ACGVJG_000707"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFJ6556685.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            13510..14385
                     /locus_tag="ACGVJG_000708"
     CDS             13510..14385
                     /locus_tag="ACGVJG_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFJ6556686.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            14651..16534
                     /locus_tag="ACGVJG_000709"
     CDS             14651..16534
                     /locus_tag="ACGVJG_000709"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFJ6556687.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(16609..16893)
                     /locus_tag="ACGVJG_000710"
     CDS             complement(16609..16893)
                     /locus_tag="ACGVJG_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFJ6556688.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(16883..17617)
                     /locus_tag="ACGVJG_000711"
     CDS             complement(16883..17617)
                     /locus_tag="ACGVJG_000711"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFJ6556689.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            17746..18369
                     /locus_tag="ACGVJG_000712"
     CDS             17746..18369
                     /locus_tag="ACGVJG_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFJ6556690.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            18561..19682
                     /locus_tag="ACGVJG_000713"
     CDS             18561..19682
                     /locus_tag="ACGVJG_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFJ6556691.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            19689..22817
                     /locus_tag="ACGVJG_000714"
     CDS             19689..22817
                     /locus_tag="ACGVJG_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6556692.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            22836..26030
                     /locus_tag="ACGVJG_000715"
     CDS             22836..26030
                     /locus_tag="ACGVJG_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFJ6556693.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(26176..26883)
                     /locus_tag="ACGVJG_000716"
     CDS             complement(26176..26883)
                     /locus_tag="ACGVJG_000716"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFJ6556694.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            27648..28046
                     /gene="spxA"
                     /locus_tag="ACGVJG_000717"
     CDS             27648..28046
                     /gene="spxA"
                     /locus_tag="ACGVJG_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFJ6556695.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            28378..29031
                     /locus_tag="ACGVJG_000718"
     CDS             28378..29031
                     /locus_tag="ACGVJG_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFJ6556696.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            29257..30255
                     /locus_tag="ACGVJG_000719"
     CDS             29257..30255
                     /locus_tag="ACGVJG_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFJ6556697.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            30431..32239
                     /gene="pepF"
                     /locus_tag="ACGVJG_000720"
     CDS             30431..32239
                     /gene="pepF"
                     /locus_tag="ACGVJG_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFJ6556698.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        10843 a         5619 c         6868 g         8989 t
ORIGIN      
        1 tgctttattc tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc tgtagtattg
       61 atcccgcgaa ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc aaatgataag
      121 gcatgttacc agcagtgaca caagacgggt cccaagatat caataaaaat gttcggtcaa
      181 cacacaaaga ggacaattcg aacatcacag atttcccacc tctatagtag catatgaatt
      241 taaaaagagc aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta gttttttgga
      301 ggatgctttt tccactcttt tatacggtaa tactgttgac ccagagcctg ttcatatttc
      361 cctgtatctt ttggctgata atactgagca ttcttcagtt tatctggtaa atattgctga
      421 ttgacccaag cctgatcaaa atgatgagga tactgataac ccacgccacg gttgagtgat
      481 ttcgcacctt tgtaatggct atctctcaaa tgatcaggaa catctccagc tttgccctct
      541 cgaatatcag caagtgctgc atctaatgcc atgtaggcag aattggattt tggcgacaag
      601 caaagatcga cgacgacgtc tgctagtggg atacgtgcct caggcaatcc caatttttca
      661 gcagctaata cagcgttcac tgtacgagcc gcagcagcag gattacctaa acctatatct
      721 tcataaccga taaccatcag acgccggcaa atacttgcca gatcacctgc ctcaactaaa
      781 cgggcgagat aatgaagtgc agcatccaca tcacttccac gaatcgattt ttgaaaagcc
      841 gaaataacgt cataatgagc atctccattt ttgtcatggg taagagcttt tctttggaca
      901 cattcttcaa tgatcgacag tgtaagatga atccttcctt ctttatctga aggagtcgat
      961 agtgtagcca gttccagccc attcaatgca ctccgcaaat cgccgttcgt tgcacgcgaa
     1021 aggtgaagaa gagcatcttc gtctaactgg attgcttgtt gtccaaggcc gcgttcttta
     1081 tcccttaaag catgttcgac tgccaactgg atgtcttgtt ctgttaatgg tttgacttca
     1141 aagatctgtg tacggctgcg gatcgctgga ttgatggtaa tataaggatt ctcagtagtc
     1201 gctccgatca agatgattcg gccactttct aaatgaggaa gcagaaaatc ctgttttgtt
     1261 ttatctaatc ggtgtacttc atccaataag aggatcactg taccactcat ttttgcttct
     1321 tccgctacaa cttgtagatc cttttttgaa tctgtagcgg cgtttagcat gcgaaatgca
     1381 taattagtag agccggcgat cgcacttgcg atactcgttt ttccagtacc aggaggtcca
     1441 tatagaatca tggaagagag catgcgagca tcgaccatgc ggcgtatgat ttttccttcc
     1501 ccgactagat gctgctgacc aacgacttca tcgatcgttc gaggtctcat gcgataagcc
     1561 aatggctgtt gcataggatt ttcctccttc ttgtttttac gtcccttaat tatatcatat
     1621 ttagcaagat gcctagaaag aacagccttc tgttgttttg ttttcatagc ttatccaact
     1681 aatattgtgt atgataaagt aggtgagaaa aatgacggat aaacaaaaga tagatgaatt
     1741 tttttggaat aaaacgacgc cagaagtggc acgggattta ttggggatgt atttggagca
     1801 tgacacacca gaaggaagat tagctggata tattgtagac gcggaagcat acctaggtcc
     1861 agaagacgag gccgcccata gctatggttt aagaagaaca ccgcgtgtgc gggcaatgta
     1921 tgaaaaacca ggtacgatct acctttacac gatgcatact catcggattt tgaacatcat
     1981 tactcagcca gaagggattc ctcaaggagt aatgatacgt gcaatcgaac ccgccgcaat
     2041 gattgatcaa atgagtaaaa atcgtggcgg aaaaactggt ccagatatca gcaacggtcc
     2101 gggaaaatta gtggaagcat tagcaattcc tcaagaactt tatggtcagt ctatagcaga
     2161 tagttcgtta cgcttagttt ttgagaaaaa aaagacacca aaaaagatca atgctttgcc
     2221 tagaatcggt atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt ttgttgtgtc
     2281 gggaaatcct tatatctctt tacaaagaaa aaaccaaatc gagaaagatt ggggttggag
     2341 gaaagaaaat gaaaaagaag gatctattaa catatttaga tgaaataatt gaaaaacaag
     2401 tgacagacta tgatgtagct ttagattggg atacaaaaaa tcatacgatc gagatcgttg
     2461 ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga tgatgcagaa gggatcgttt
     2521 cgcaagaacc aattatcgaa tttgaagacg gcgtacttct ttttaatcct cagaaatctg
     2581 tttttgatga acgcaattat ctagcagtaa tcccttatga aggaaaaaaa gggttggcaa
     2641 aatcagtagc tgattcttta gtggaatatc taaatgaagt attagctcaa gggcaaagcg
     2701 atttgcttga ttttttggat gaagacgatg acgaagctgt attcgagctt cactgggata
     2761 atcagcaact agcagaatta gtagaagtaa aacagcaaaa tgaagcagat acttatttac
     2821 cgtacccaag ttattaagga gtgacagata tgaaatggaa tgaagtaaaa gtagaaactg
     2881 aaagtgaagc agtcgaagca gttgctaata ttttgatgga ggcaggggcg agtggtgtcg
     2941 cgattgaaga ttctttagat gttgaacatt ttcagtcgga tcaatatggt gagattttag
     3001 ataaaaatga ttttacatcg ctgaaagaag gcgcactagt catggcttat tttccggaaa
     3061 cgactttctt accggaaatc ttgcctttta tcaaagatcg tatcacacaa ttacctgagt
     3121 ttggtctagc tattggaaaa aataaagtaa cagttagtga agtagaggaa agcaactggg
     3181 caacagcctg gaagaaatac tatcatccgg tgcgtattac gcgaatgctg acggttgcgc
     3241 caagctggga aaactaccag acagaagatc ctctagaaaa aattatcaca ctagatcctg
     3301 gtatggcatt tggcacaggc actcatccca cgacttcctt gacattgaca gcactagaaa
     3361 cagtcttgcg tggtggtgaa acattgctag atgtgggaac tgggtcgggc gtgctaagta
     3421 ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt tgatttagac gaagtagccg
     3481 ttcgttcggc gaaagaaaat atggatatga atgaaatagc caaagatgtc catgtatcag
     3541 ctaacgatct tttgacagga atcgagattg aaagtgatgt aatcgttgcg aacattttag
     3601 cagatatcat cttattgatg atcccagatg cttggcgttt actgaagcaa acaggcacgc
     3661 tgatcgtttc aggaatcatt gaagagaaga agcaaatggt tctggatgcc atgttagaac
     3721 aagggtttat cgtggatcaa attttccagc aaaaagactg gtatgcaatt attttgaaaa
     3781 aaccagaggt ggaatagatg cagcggtatt ttttaaaaga atcatatgaa tcaaaagact
     3841 attttgtttt agaaggggaa aactatcacc acattgtacg ggttatgagg atggaacccg
     3901 aggatgaagt ttttttggca ttcaatgatc aaatagccat tgttgcagaa atcacggaga
     3961 ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa aaggaactgc
     4021 caagttcgat taccatagcg agtggctatc ctaaagggga aaagctggat tggatcgttc
     4081 agaaatccac tgaattagga gcgcaagctt ttcttggttt cccggcatca gcctctgtcg
     4141 ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag attgctcaag
     4201 aggcagcgga acaatcccat cggcagatcg tgccatcagt agagttattg gaaacgaaac
     4261 aagatttatt tgagcggcta gatgattatg acgtggtctt ggttgcttat gaggaatcag
     4321 caaagcaagg cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa ccagatagtc
     4381 gaatcttagc tgtctttgga ccagaaggtg gattatcccg cgatgaagtc gattattttg
     4441 tagaacacaa agggatactt tgtggattag gaccaagaat ccttcgtaca gaaacagcac
     4501 ctctttattt attaagtgct gctagctatc aatgggaatt aatgaatcct tctttcagct
     4561 aacgttcttc attaataaaa gagtatcgac agaaagaatt gaaaaaatag tcaacaattt
     4621 gtataataga aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa aagcttttaa
     4681 aaagcgaaca tttccgtaaa aaaacgtata actgcacgga ttttgggaca attatcaaac
     4741 agatatttgt cccgattccc gttttaccga aaaaagaaag ggggatcgat tcctgtgaca
     4801 aaagaagaaa ttttaacggg acccggtgtc ataaagctcg ttagtcaata catgggtccc
     4861 gaacatgtag catttgttga gaaagcatgc gaatacgcga ctgctgcaca tgacggacag
     4921 tttagaaaat caggcgaacc ctacattatt catcctatcc aagtcgcagg tatattagca
     4981 gatttaaaaa tggatcccca tacagtggct acaggcttct tacatgatgt tgttgaagat
     5041 acagaaatca ctttagaaga tctgagagaa gaatttggcg atgacgttgc tatgttagtg
     5101 gacggcgtaa ccaaattagg gaaaataaaa tataaatccc acgaagagca gctagcagaa
     5161 aaccaccgaa agatgctgct agcaatggct caagatttac gagttatcat ggtcaaatta
     5221 gctgatagac tacataacat gcgtacgttg aagcacctgc gagaagataa gcaaagaagg
     5281 atcgctcagg aaactttaga aatctatgcg cctcttgcac atcgtctagg gatcagccgg
     5341 atcaaatggg aattagaaga tacagcactt cgttatctaa atccaaaaca gtattaccgt
     5401 atcgtccact tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag tggtactgtt
     5461 gaagatattc gaatagcgac agaagagctg gggatttttg cagaaatcta tggacggcca
     5521 aaacacattt attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt caacgaaatt
     5581 tatgacttgc tagctattcg agtgatcgta gattcaatca aggattgtta tgctgtatta
     5641 ggagcaatcc acacaaaatg gaaaccaatg cccggcagat ttaaggatta tattgccatg
     5701 ccaaaagcta atatgtacca atctttgcat actaccgtga tcggtccggc aggaaatccg
     5761 gtagaaattc aaatccgaac acaggaaatg catgaaatcg ctgaattcgg ggttgctgca
     5821 cactgggcct ataaagaagg aaaaaatgaa aaagtagaac cagatggtat gacgaaacaa
     5881 ttaagctggt tccatgagat actcgaactt caagacgaaa gctatgatgc ttctgaattt
     5941 atggaaggcg taaaaggaga tatctttagt gataaagtct acgtcttcac accaaaagga
     6001 gacgttactg agttaccaaa aggatccgga ccattggact ttgcatacag tatccataca
     6061 gatatcggta acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca gcttgattac
     6121 aaattgaaaa acggagatat cattgagatc atgacttctc caaattcatt tggcccaagt
     6181 cgcgactggt tgaaattagt tgctactagc aaagcaagaa ataagatcaa acgtttcttc
     6241 aaagcccaag atcgagaaga aaatgtgatc aaaggccacg aatccgtggt caaatgtatt
     6301 acagatctag gatttacgcc taaagatatt ttgacgaaga acaaactgca agaagcactc
     6361 gatcgtttta attatcaaac agaagatgat ctctatgcag ctgtagggta tggagaagtt
     6421 agccccttga cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca aaaaatcgag
     6481 cagcaaaagc aagaagcaga agaaatcatg aatcagccga aaaaagaacc tgacaaaatg
     6541 aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag aaaacttact aattcgtatc
     6601 agtcgctgct gtaatccgat tcctggtgat gatatcgttg gttatatcac taaaggcaga
     6661 gggatatcca ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc aaatgtagca
     6721 gaacgtttga ttgaagtcga atgggaagat acatcgaata cacgaaaaga gtatgatgca
     6781 gatttggaaa tttacggcta taatcgttca ggcttattga atgatgtact tcaaacagtc
     6841 aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga cgaataaaga taaaatggcg
     6901 acgatccatt tgacggttgg tatccagaat ttatcccacc taaagagtat cgtggataaa
     6961 atcaaagcag tacctgatgt ctacagtgta cgccggacga atggatagga ggacagatat
     7021 gagagcagtt gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc aagaaattgg
     7081 aaaaattggc tacggcctac ttgttttatt aggcattcat gaaaccgata cgcaaaaaga
     7141 tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg aacaaggaaa
     7201 aatgaatatt agtgtagaag atgtgggagg acaaatttta agtatctctc aattcacact
     7261 gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac gaccagaaac
     7321 agctattcct ttatacgaat cattcaatga aggaatcaga agtcgcggca tcacagtcgc
     7381 taccggacaa ttcggagcag atatgtcggt ttctctcgtc aatgatggac ctgtaacgat
     7441 cattattgat tctaaaaaca aataaagtaa tctggttttc actagtaaaa actatgaaac
     7501 acgtcgctta agttagactg ataaactcta acttaagtgg cttttttgtc tgcaaaaagc
     7561 gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt tattttttat
     7621 aggaggttgc tgaagatgaa agaagtacca gtagtagata tcaaaatcac agatttaaaa
     7681 aaattgtatc aaaaaggata caacatgttg gaggagttac gccatgaagc ggacgctccc
     7741 gtcgtcaaag caaagatttt caataaagaa gctatcacga tctacggatc atcagcagca
     7801 aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa attagtattg
     7861 aagacacttt ttggtcaagg tggagtacag actttggatg gagctgctca tcaccatcgg
     7921 aaaaatattt ttatggatct aatgacaccg gaaagaatgg aagattatca tcgtatctta
     7981 gataagaatc tgacacaagc acttgaagca cagcatggtc aatttgaact atttgattta
     8041 agtaagatgg ttttctttac atctatttgc gagtgggcag gtatcaattt atcagcgatt
     8101 tctaaagatg aggtcgaaaa attagcagaa tatcaaatct ctatgatcag cggaacgttt
     8161 acttctccga ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga aaaatgggcg
     8221 caaggcttga ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa tgtggcatta
     8281 tatgcttttg ccaatgccac agatttagat ggtcagcttt tacctctgga agtggcagct
     8341 gttgaattat tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc ggcattgatg
     8401 ggacatgcac ttttcagtag accagatttg tatcagcaat taaaaaatga cttttcgacg
     8461 ttacaagatc cgtttattca agaaatgaga cgctattatc ctttcttccc aatgctacct
     8521 gcgatttcgc tgaaagaagt agaagtagat ggttatcgta taccagaagg cagttgggtt
     8581 attttagatt tatatggaac agaccatgat gaacgaacag ttgaagcgcc agattccttt
     8641 atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga atatgagatg
     8701 attgcacaag gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga atggatcact
     8761 cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt ctctgtacct
     8821 gaacaagatt ggacgattcc attcaatcag tttccgactt atccgaatag ccgagcgcta
     8881 ctttacaaga attagattag cataaactta tcgaaaaaaa ggaagatcaa acagtgctga
     8941 tcttcctttt ttcatattac tataaattga taaggtcaat tgatcaaaat ccacgaaatg
     9001 cttcatcaat ggtttgataa tcttcattgg ataaatgcaa atctaaagct tggacattgt
     9061 cagatacttg ctctggacgc ttagctccag gtataactac agacactcgt ggattcttca
     9121 tgtaccaagc taaaacaagt tgcgcaggag tggcttggta tcttttagca atggggcgaa
     9181 gtacatcgac agcggttaaa atttctctga aacgttctcc ttggaaatct ggatttcttt
     9241 ttcgaggatc tccttcacca aattgttttt cctcaccaag ttcatatttt cctgtcagca
     9301 aaccggaagc aagaggaaaa tagggaacaa aactgatttt gtttttttca agataaggga
     9361 ataattcttt ttcagcctgc cgatggatca aactgtattt atcttctact acatcgacat
     9421 atccatctgc attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca ccgactgctc
     9481 ggatttttcc tgcttctttt aattcgtgca atgtagcaac tgattcattt tttggtgtgc
     9541 tttcatccgg aaagtggata taaaaaatgt cgatgtaatc agtttgcaga cgttttagtg
     9601 catcttccac cgattgtttg agaaactcag gagaattatc aaatgttcgc cctttatttg
     9661 gtacatgagc agcttttgta gcgatgacga cacgtgagcg atcatattct ttttccttca
     9721 gtacctcgcc aatcagttct tcggaacggc cgtttccgta agcataagcg gtatcgatca
     9781 aattgatccc gctattcaaa gctgtacgaa ctacttgttt tcctgtctca tcttccaaac
     9841 cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa tccaagctgt tctgcgtaga
     9901 cttgtgaatg tccgattctt atttctgcca tagctgattc ctcctttttg tctagtctaa
     9961 aacaaaatac caaaaagctg aactaaaacg tttttgtgag gggaaatagt cagatatctc
    10021 ttgactttga gactggtttt tagtaggtta taagtaatat cgaatgtcaa tgaaagagcg
    10081 agtaagtttt tgaaacttta caagagagga tcatcgaggc tgaaaatgat ctaaagtttc
    10141 attagcctaa agacactctg gagatggacg agtgaaaagc cgtcccgcgt acgagcgtta
    10201 ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga atttcattca
    10261 cccttgttca tgatgaacaa gggtgatttt tttatttaca tagctaagaa agggagagaa
    10321 aactatgagt ttccaaagac caaaaggaac aaatgatatt ttgccaggcg aatcagaaaa
    10381 atggcagttt gtcgaagaaa cagcacgttt attattcaga gattatcagt ataatgaaat
    10441 gcgtacaccg atttttgaac actatgaagt gatatcccga agtgtagggg atacaacgga
    10501 tatcgtttca aaagagatgt atgattttta tgacaaaggg gaacgccatg tgacgcttcg
    10561 accagaaggt acagcaccaa tcgttcgttc ttttgttgag cataaattgt tcggcccaga
    10621 gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg tttcgctacg aacgccctca
    10681 aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa gcatttggat cagaaaaccc
    10741 tgcaactgat gtggagagca tggtgatggc acttgatttc ttcaaacagt tggggatcac
    10801 gcaaatccgg ctagtcatca attcattagg tgataaagaa acgcgtcaag cctatcgtca
    10861 agcattgatc gactatcttt taccttttga atcagagctg agtgaagact ctaaacgccg
    10921 ccttcatgaa aatccattac gcgtattgga cagtaaagac aaacgagatc aaaaatttgt
    10981 ggcagaagct ccatctatct tagattatct aagcgagcct gctcaaaaac attttgatac
    11041 cgttgtttcc atgctggatg cattggctgt tccttatgaa atcgacagta atatggttcg
    11101 cggactagat tattacacac atacgatttt tgaaatcatg agtgacgcac caaaaatggg
    11161 ggcacaagca acgatttgcg caggcggacg ttatgacaat ctagtggaag aactaggtgg
    11221 tccttcaaca cctggcttcg gatttgcaat gggtatcgag cgattactct tgactatgga
    11281 ggcagaggaa gtagttatcc ctgcatttaa tgagttggat gcttacgtgg tcgcactcgg
    11341 agacgaaaca aatatcgaag cattgaaagt ggtccaagcg attcgaaact ttggtttttc
    11401 agctgaccgc gacttcatga accgtaaagc aaaagcgcaa tttaagacag cagataaagc
    11461 gaatgccaaa ttagtattgg tgatcggtga agatgagctt gctagtcaaa cgattaatgt
    11521 caaagcaatg gcaagccgaa aagaaaaaag ttttccatta gaagagatat atgaacattt
    11581 tgatgaagtc tatgatgaaa tgactttatt cgagtaggag gaaaaaaata aatggcacaa
    11641 agaacgatct attgcggatt agtttccgaa caacaattag aacaaacagt tacattaaaa
    11701 ggatgggtac aaaaaagacg agaccttggg ggagtaatct ttatcgacct acgtgatcgt
    11761 gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg ggaaatcgcg
    11821 gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg aagttagaag acgcggagaa
    11881 aatgcagtaa atcctaaaat ggccactggt gaattcgaag tgattgcaag tgaaatcgtg
    11941 attttgaatc aagcaaaaac cccgccattt caaattgaag atgatcaagc aatcagtgac
    12001 gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac taaaaacatc
    12061 aaattaagaa atgcgacaac aaaagcgatt cgtcattatt tagatgataa tgactttctc
    12121 gatatcgaaa caccttacct agggaaatct acaccagaag gcgcaagaga ctacctagtt
    12181 ccttcacgtg tccatgctgg acatttctat gcgttgccgc aatctcctca attgtttaaa
    12241 caattgctga tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg tttccgcgat
    12301 gaagatttac gtggagaccg tcagcctgaa tttacgcaag tagatattga aacaacgttc
    12361 ttaacagcag aagaaatcca aacttacaca gagggattga ttgcgaaggt aatgaaagaa
    12421 gttcgcggta tcgaagtcac attgccattt cctcgtatga cctatgatga agcaatggct
    12481 cgctacggca gcgacaaacc ggatacacgt tttgatatgg aattgatcga tctaagcgat
    12541 acagtcaaag aagtagaatt caaagtcttc caaatggctc ttgaaaacgg cggtgtcgtg
    12601 aaagcattga atgctaaagg cgctgcagat cgctactcac gaaaagacat ggatcagtta
    12661 ggtcaatatg taggacaatt cggagctaag gggcttgcat ggctgaaagt agaagaagac
    12721 ggattgaaag gtccaattgc taaattcatg ggagaagcaa cagaagcaat cattaaagca
    12781 acagatgcaa aaccaggtga cttgctgatg tttggcgcag ataaaagtga aatcgtcgct
    12841 gctgcattag gtgctatccg gacacgttta ggaaaagaat taggactaat tgatgaatcg
    12901 aaattcaatt tcttatgggt aactgattgg ccacaatttg aattttcaga agaagaaggt
    12961 cgatatgtct ctgctcacca tccattcacg atgccaaaag aagaagatat cccgttattg
    13021 gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa cggttatgaa
    13081 ctaggcggag gttctatccg tattcatact cgtgagcttc aagaaaaagt actagaaact
    13141 ctaggcttta caaaagaaca aatggaagaa caatttggtt tcttgttaac agctttagat
    13201 tacggattcc ctccacatgg cggtattgca ttaggattgg accgttttgc aatgttatta
    13261 gccggagaag ataatattcg tgaagtcatt gcctttccta aaaacggaaa agcagcagat
    13321 gtgatgagtg atgcaccaag tatggtttcg cctttacaat tgttcgaatt gaatattgat
    13381 gtaacgaata tagaagaata gccgataaaa aggtaaaaag gatttgtcga atacgcttct
    13441 tttgacaacc ttgatttaat tgaaaaacac tttataatga agccataaca tataaagcgg
    13501 ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc atgattacac gaccaagctt
    13561 tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt ttatcagcca
    13621 ggacatattt attcaagtgg tattactggt ctagctcaga ttttgacgac tttgtcaaaa
    13681 tcactgatag gattcgaagt accggtatca acaactttgt atttactgaa tatacctttg
    13741 tttgtattgg cgtggttaaa aatcgggaag aagtttacaa ttttcacttt tatcacagtc
    13801 actttgacct ccctttgtat ccatatttta ccggaaacag tcctatctac cgatccaatc
    13861 atctgtgcgt tgtttggggg ggctgtgatg ggactaggta ttgggctagc gttgagaaac
    13921 ggcctatcat cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa aacaggaaag
    13981 tcgattggtt ctatttcgat ctacttcaat gcattgatcg tttttgttgc cggaattcta
    14041 tttggctggc aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg aaaagtaact
    14101 gatgtcgttt atactaaaca aaagaagatg caggtaatga tcgtaacgaa acatccagaa
    14161 aaagtagtaa atgaaatcca aaactgtatg cgtagaggga tcacgatcat caatgaagca
    14221 gaaggtgctt ataagcatga taagcagaca gtattgatga cgatcgttac acgatacgaa
    14281 ctgcctctac ttagagaagc aatgaaagct tctgatccac aagcttttgt aagtattgca
    14341 gaaaacgtcc agatacttgg tcgtttctat gaggatggca tttagatcga gtttgacaaa
    14401 aattcatgaa atactgctgt agacagacgt tcgtttgtct gcagtttttt tctaccatag
    14461 tagaatcagc aaagaaaaac tgctttttta tttgaaattt tcttaaagtt gaaaaaaagc
    14521 atgtttattt ttttaaattt atgggatgat agaaaaaaat agtagagaaa ggacacgatt
    14581 ctacttgcta ttttcactta atacccgtat attacgaatg aaaaaagttt atgaaaagtt
    14641 ggaatattga atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca ctgggtttga
    14701 tgggctccgt tcattagctg tggtgggtgt tatcttctat caccttctgc caactagtat
    14761 gaaaggcggc tatttgggcg ttccgatttt tttcgtcgta tctggttatt tgatcacgga
    14821 tctgctgcgg caggaatggg aacaaaatgg aaaaatcaat atttggcaat tttatgtcag
    14881 aagaatgaag agactctatc cggcacttgt ttttatgctt gtgacagctt cggcttatat
    14941 cactttgttt cagcgagggc tgttaaacaa tcttcgagga gttgtggtca gcagcttact
    15001 gtatgtcaac aactggtggc aaatcaataa cggtttatct tatttcgatc gatttgctaa
    15061 tgagtcaccg tttacgcata tctggtcttt agcagtagaa ggtcaaaact atttggtttg
    15121 gccgatcttg tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga tcttttatac
    15181 tgtgttaggt acatccttcc tctccgccat cttgatgatg atcttgtata caccaggagg
    15241 cgatccaacc cgtgtttatt acggtaccga cactcgtctg ttttctatat ggttgggaag
    15301 tgcgcttgcg tttgtttggc caagtacgcg tttaaagaaa aacatcccga aacaagcgaa
    15361 aaatattctg aatatcgctg gaggaatctc gcttttggca ctgattcttt gcttcttctt
    15421 tttagatgat cattacaatt tcatctatta cggaggaatg ctcctagtga gtctgttatg
    15481 cgtagtgcta gttgcagtga cagcacaccc cggagcaagt ttgaatcgat ggctgacaaa
    15541 tccagtattt acttggattg gcaaacgaag ctatggcatt tatctgtatc agtttccagt
    15601 tatgattttt tatgaagcaa aggtgactga tattgccgat catgttttgt tgcatacttt
    15661 agtggaaatc acattgattc taatcatcag tgaactttct tatcgatatg tggagcgtcc
    15721 gctagcacgt tttgattatg gtcgtacatt ccaagtgatg aaaggatggt tcagcaaacc
    15781 gatcatcagt aagagcaaac catgggttgt tccaggcaca ttacttgtag caattgcact
    15841 cgttggtttc gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac aactgcaggc
    15901 aaaaatcgct gaaagtaaaa aattagcaga agaaaccaga aacaattcag aaaacaccac
    15961 tagttcggtt gaccaagcga tccttgataa gtacgagttg tccaaggcac aagggaataa
    16021 ggcacaaaaa ttagaactaa cggcatttgg tgattcagtt atgttggatg ctgcaacgga
    16081 tctacaagaa ctttatccaa aagtagtggt agatggagat gtcggacgac aattatatgc
    16141 cagtgtaccg tacattgaaa aattaaagga acaagatttg ttgaaagaca ctgttctgat
    16201 cggactagga acgaatggct cattctcgga agctcagttt gatagtgtga tggatgcaat
    16261 cggtgatcga aaagtatact gggtcaatgt ccgagtaccg acgaagcgct ggcaaaatga
    16321 cgtgaatgcg atgcttgaac agatgacgaa aaaatacgac aatatgacgc tgattgattg
    16381 gtacagctac agtaatgacc atagtgactg gttttatgat gatcaagtcc atccaaatcc
    16441 tgaagggatg acacattaca ttcacttagt cagcgaagcc ttgctaggag gaagttcaag
    16501 tacgaacagc agtgagacaa acgtgacaga atgatagcta caaaaaactt caggcaaaaa
    16561 catttgtttt tgcctgaagt ttttttatat ttaccgtgtt tttaacattc atatagattc
    16621 aggaagcaaa acggttgagt gtgtctgtaa ataacgttct ttttgttttc gggtgagttt
    16681 tttgaatgca gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt ctgcatggat
    16741 catcttaaca ggtcttcttt tttgaagacg agtatattta gcaccagtac cattgttatg
    16801 ttcctctaat cgtctagaaa gatcggttgt atatccacca taaaatgtct gatctttaca
    16861 gaataatacg taaaaataat gttcatttgc catagagcat ctcacgaatc tctgcagtgt
    16921 attctccatc catattgtaa gtgatcaatg gtggagcgac tttgaatccg tctaatttgc
    16981 cttctttgat tccttcaact aacaaaatat ttgcctcttt tccaggtttt gggtaaacaa
    17041 attggatcct tttaggtgca attcgatagt cttccatgac ctgaatgatt tctaagaacc
    17101 gatcaggacg atggaccata gtaaagcgtc catttgtttt tagtagctta cttgaaatat
    17161 cgatcacttc ttctaacgtc gtatgaatct catgacgggc gattgccagg tgtttattag
    17221 ggttcgtttt atttgttggc agccctttga aataaggtgg attacagacg actaagtcac
    17281 aagaattatg tcgaacgaca gataagctgt ctttcagatc gatggtgtgg acagtcattt
    17341 gttcatttaa atggttaagc gcgatactac gtcttgccat gtctgctaga cgaggttgaa
    17401 gttcgatagc gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca actgcgccat
    17461 ttcctgcaca aaggtcaaca atcttccctt ttttaggtac acgggaaaag tgagcaagca
    17521 gcacagcatc taaagaaaaa gaaaacactt tggaactttg gataatcttg atatcttctg
    17581 cgtataattg gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa aatgaaaaaa
    17641 atagtcgtct gtattatact atttttatta aaaagaacca aggtggcttg tcaaacctct
    17701 attttttatg catactatga ccataagaag ggaaaggaag agactatgtt ttatcgattt
    17761 atgcgcgggg tagttcgagt actattagcc ttagttaatg gaaatgcccg ctatgagaat
    17821 aaagaagttt taccgcaaga tgagaattac atactagttg ctccccatcg tacttggtgg
    17881 gacccattat atctggcggt agctgcagca cccaaggaat tcagctttat ggcaaaagaa
    17941 gaactgttca aaaatccagt tctgcgattt atattaactc gatccaatgc ttttccagtt
    18001 aaaagagaaa atccaggacc aagcgtcatt aaaacacctg taaagatttt gaaatcgaca
    18061 aatttgagtt tgatcatgtt tcccagtggg acaagacatg ctacagaact taaaggcgga
    18121 atggcattga tttccagaat gtctaaagcg aaaatcgtgc cagctgtata tcaaggtccg
    18181 ttgacacttg gggatctgtt caagcgtcag agagtgacag tgcgtttcgg cgaaccaatc
    18241 gacttatcag atatcaagaa aacggataaa gaaggtatgg cggagatcga acgacgaaca
    18301 caagcagctt ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata tgaagtcaaa
    18361 aaaaaataaa atgataagtt tatgcgtggg cggtcttttg aatctactgt cctgttcagt
    18421 tcagactttt tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt ttgcgttcca
    18481 atatgtaaaa tagaagacag gtgtcatgta atggatcaaa gtgttataat aattagaagg
    18541 acattttttg aggtgattga atgcgttttt tacatacagc cgattggcat atcggaaaaa
    18601 aactacaagg ttatgacttg attgaagacc agcgtcatgt actatcttct atactagata
    18661 tcgcgaaaga agaaaaagta gaggcaatcg tgattgccgg agacttatat gataggagtg
    18721 tcccagctgt tgatgccgta gaattataca atgaattgat ggtcgattgg aatttaaagg
    18781 aaaagatccc gatttttgct atatcaggaa atcacgatag cagtacgaga ctttcagcgg
    18841 gtacgccatg gttcagttat tcgaactatt ttttgcgaac acagctagca gaagcatttg
    18901 aaccagtcga gtttaaaaac gtacaatttt tctttcttcc ttactttgaa ccaattgcag
    18961 cgagattata ttttgaagat gacaatatcc gaacgatcaa gcaagcggta gccaaggtga
    19021 tagataaaat gcaggagcta tttgattcag aaaaaaaaca agtattgatt agccattttt
    19081 ttgtaatggg aagtgtaaaa tctgattcgg aaacgaaaat cgaagtaggc gggttggatt
    19141 cgatccctgg tgaactactt acaccgtttg attatgtagc actaggacat ttgcataatc
    19201 aagctgcttt gagacaggat aatgctcgct atagcggttc cccattgaaa ttctctttat
    19261 ctgagatcaa ccaaaaaaaa ggcgtatgga tcgttgacct gacaacaggc ctggagttga
    19321 attttaggga gatcgaaccc ctgcatgata ttgaagaaat aacagcgagt ttcaaagaat
    19381 tgctggatcc tgccttttat cagtccgtca atcgggagaa ttacatctat atctatctca
    19441 ctgatagaac gatcatcccg aatatgatga accagcttcg gcaaatatat cctcgtattt
    19501 tggggattga acgggcaaac ggtcgagcag aacaaacaaa gcgcaaacga aaagtactag
    19561 aaatgaaaga tcctcaaaaa ttagcagaag cattttttag tgaaatgacg gaagagcagc
    19621 taactgcaaa ccaagaaaaa tggctcggag aaacgttgca agaaatcatg gaaatggaat
    19681 gatcaagaat gaaaccgaaa aaattacaac ttaaaaattt tggtcctttt atcaatgaga
    19741 ccgtagattt ttcacgtttg acagaagcac cgcttttttt gatcagcggg aagacaggag
    19801 ctggcaaaac gacgattttt gatgggatca ccttcgcact ttttggtgaa acttccggaa
    19861 gacttcgctc tggaaaagag atgcgttctt tgtttgctac accagacgaa gaaacaagcg
    19921 tgactttttc ttttgaacat cagcagatga gctatgaaat cgaacgaaaa ccagaacaag
    19981 ttttagcaaa acgaaaagga aacggaacaa aaaaacaggc agccaaagta acgttgacca
    20041 tttttgatgg gaaaggaaaa gaaataaaac aaatcacaaa acgtacagaa gcagatcagt
    20101 tgatcagaga actgatgaat ctcgatgcga aacagttttc tcaaatcgtt ttactacccc
    20161 aaggagaatt ccgtaatttt ttggtttcat caagcagtga taaagagact gttttacgtc
    20221 atttattcgg tactcaattt ttccaacagt tcaatgagcg attaaaagaa aaagcaaaac
    20281 aacagcaaca aaaattggat catttagagc aagagctgca tcttctgcaa aaaaggtttg
    20341 ttttagtcaa agaagaggaa ccagcagcag ggtttgaagc cgttttagcc caatggacaa
    20401 accatcaggc aatactcgaa aaacaaatca cggaagaaaa agaagctcta acggaacttc
    20461 aggaaaaaca aaagcaacta gaaaattcct actatcgttt tgaaagcttg aaaaaaagtt
    20521 atgaagaaaa aacgatatta ataaacaagc aaaaggaatt agctgctcaa aaagacgaaa
    20581 tagaggaaaa gaaacgatgg attgggtatt atgaatttac gcaacgatta gtcgaaccaa
    20641 tccagcgcct acaagaaata aaagttgaaa aaatagaact gtcacaaaaa agtagtgagc
    20701 aaacaaagaa actagcgatg actacgcaga attatgaaga gtggcaaaaa aatgaagcaa
    20761 accgcgtacg ccgacaaaca aaaatcaaag aagctgctca aaaactgcaa aacaaacaga
    20821 tgatggtgcc aattgtcgaa tccttacgta gtaaaaaaca agacgcagaa caattaacaa
    20881 tttcattgga taaagaagaa ttgaatgccg agaagctgta tcaaaatctc gctatctatc
    20941 aggaaagaac tcgaattctt caagaaaatt tactggatca ggaaaaactg caagaagatc
    21001 gtcttgcttt tggacagtta aaatatctag atgaaaagca gcaagaaata gaaaaacaaa
    21061 aagcaaaaga cactgggtcg ctcgcacaga taagagaaga actcaaaaaa gcaactgaag
    21121 aaataacaaa aaatagtcag ctattgaaag aacagacaga acaagcagaa cagctgaaaa
    21181 gccaatgggc aaagcagcaa attgctcgtc ttcagctttt tttgcgtcca ggggagccat
    21241 gtcttgtttg tggatcaacg gaacacccca tacaacaatt aaaacacgaa cagccaaata
    21301 aggaagatat cttaaaagta gaacaacaac tggcagaagc tgagtctgct gtagaaagaa
    21361 ctcagcaaat cattgccaaa agtgaagcac atgaaaataa tcttcaacaa caattatcag
    21421 atttagaaca aaaaatcatg gcaacgaata tacaattggc tgaacaagaa gaacacttgc
    21481 aacaggcttt gaaaaaggca tttcctgaga taacagaaca aaaaacaaaa acaactcttg
    21541 tagtaatcga acaatcttta gatagacgaa aaagcaagat ggccaatgca agaatagagc
    21601 tggaagaaat aagacaacaa gcagaaaagc tcgaacaaca attagctgtc tacaaacagc
    21661 agcagacaga aaaaaataat cagctccagc aactgcaagg agaaatcaaa agtttagaac
    21721 aacaattgca agatacggat agtaactgtt tagaagagga gatacgacga cttgaaaaac
    21781 agttagaaga agactcgaat caactagctg ttgagcagaa agcaggagtg caattgttgc
    21841 aagatcttca tacgcttcgg acacagcttg atcttcaaca tgaacaaata gaaaaaatag
    21901 atgaaaaaat agaaaaggaa caagaaagaa tagctaaaaa gttagaaagc cagtcgtatt
    21961 tttcaacaat tgaagaaatc tgtgcatttg cacaacagca agcacaatat aaagaaaacc
    22021 aacgtacagt tacttcttat catgaagagc aattagtgac agctgaccgg ctgcgtcaat
    22081 tagaaaatat ccagctaact gagacattcc cagatacaga agagatcagc caacagttag
    22141 aagaaataag acgtatgatc gaaataaagc aagaaaagat atacggttta caagaacaaa
    22201 aaattagtaa tcaaaaaatc tacgatgaat gtctttcgat ttatcacaca agccagacga
    22261 aattagatga gcttacgcaa ttacagcagc tttcgcaaac attcaacgga gaaaatccta
    22321 agaagactag tttggaacgg tatgtgcttc aggtttatct tcaagaagtg ttacaagtag
    22381 caaatgatca tctccaacgt ttaacgaaaa atcgttatca atttgagcta gcggatacca
    22441 ttggcagtta tcgagggaaa accggattag aaatcaatat ctatgatgat caggcaggta
    22501 tgtctcgaag tgcgcatacc ctgtctggcg gtgagagttt tattgcagca ctttctttag
    22561 cattagctct tgccgatgtg atccagacac aagctggtgg tgtagcaatc gatgcattgt
    22621 ttattgatga aggatttggg tcattagatg aagaagcttt ggaaatggca atggaagctt
    22681 tagaaacgat cgaaagtgaa ggaagaatga tcggcatcat tagtcatgta agagaattga
    22741 aagaacgagt attacaacaa ataagaattg atacagaagg tagcggacaa agcaaaatac
    22801 gctatcaact tggataaaaa gggagattgg taactatgaa atggagtatt ccagaaaaag
    22861 tcattgcacg tggacgtcaa tacttgcaag aaggccgagt gttatcggtt gtccctgacc
    22921 aagaagaaaa cgtgtggcat gcagaagtct tgggcagtga attgtatatg gttgatttgg
    22981 atggtacagc aaaagagata gattactgcc aatgtcccta ttgggaggaa cataaatatt
    23041 gtaagcatac agttgctgtg gaactttatt tacggtcgaa aaatctatct cgtatcatga
    23101 aaaaagacca gccaatgcta gaaccggtaa aagcaacaag tgagggagaa atcattgcaa
    23161 aaggtttttc tcgtctaaaa acacaaaaca atcctcaagc ggtgcaacct ttgattattg
    23221 aataccaagt agaaacgatt gagactaatc agtatcaccc agagctttct attttggcac
    23281 tttacttgcg tatcggttca ttggcatcac cgaaaaaaac atatatcgta aaaaacatct
    23341 atgatttttt acaagcttac acagaaaaag aaacttacac tgttaataaa cagtaccagt
    23401 ttcgtctaga taaacgggcg tttcaaaaag aagatcaaaa aatactcacg catcttgcag
    23461 gaagtgctca gacgcatcaa ttattaggta cgaacggctt acaagtcaaa ggaaaactag
    23521 ataaaagata tctgcttctc ccagtagaac aagcccgctt cctgttagag cagatgaatc
    23581 agactacacg tttgcatctg cagataaatg aacaaaacga tcaaggaata caattttccg
    23641 atcaaaacaa acctttatca ttcaaagtgg aaaaatttgg agaagattat ttgctgcaag
    23701 cattgaatga ttttgatttt ttctttatgc attatcagtg gggagttatc caagggacat
    23761 tttattctct gacgaaatat caacaaacgg tttatcaaac ttggaaacaa ttgattcgcc
    23821 gattggagaa accagaagta gtgttcaaga aaaaagaatt accgtctttg tttaaagaga
    23881 ttatccctct gctgtcagag atcggcgacg tagctattgc ccctgcagta gaagaggaag
    23941 tagcagatat ccccgtcgaa tttattttct ttttccgaaa agcaaaagga aaaatccaag
    24001 ctcgagtcga tttcgtctat ggcgaagtca tctattcgac tgacccgaag catgaggtct
    24061 cgacagaaca ggcttttcag gtgttacgtg acacagtaga agagaaacga gtacttgatc
    24121 tattcagaat gtatcgctac cagaaaaatg aaactggtta tgaacgtcca ttgccaagcg
    24181 gggaagaact atatgccttt ttccgcacag aactatctgt cttccgtcag tttggtgaag
    24241 tcagacttgg aagaaaattg cgagagttgt atttagatgc gcataaacat caaccgaaaa
    24301 tcgaagtaac cgagaccggt tcatggctcg atattcgatt cgatgtgaca gggatagagg
    24361 agcaagaaat cgatcatgtc ctgcaaagct tgctgagaaa tgatgctttt tatacattag
    24421 aaaatggtca ggttctttct tttgattcag aagaatttca acaaacaagc caagtgttgc
    24481 aacaattacg ggaatcgata cgtacagaag aaggaacgat ccatgtacct aaaaatcaag
    24541 gattgatcat tcaaaatcaa ttggaaaaaa gcaatgctac ttttagtgaa tcgttccaaa
    24601 cgatggtaca agatctcatt catccagaaa gataccaggc acagcttccc aaaggattga
    24661 atgcaacgat gagagattat caaaagcaag gttttcgatg gttgaagatg ttaggtcatt
    24721 atcagtttgg cggtatttta gccgatgaaa tgggacttgg gaaaacacta cagacaattg
    24781 cctttttact ttcggaaaaa gaagaacgga aaagcttctc tgctttgatc gtagcgcctg
    24841 cgagcttaat ttacaactgg caggcagagg tgagaaaatt cgccccttct ttaagtatcc
    24901 aagtaatcaa tgggaataaa aaagaacgtg aagaattact ggctaaagac acagatatcc
    24961 gagtgacttc ttatgccagc ttaagacagg atctagctaa ttatcagtcg caaaaaattg
    25021 attatttgat tcttgatgag gcacaaatgg tcaaaaatag cagtacgaaa actgcacaag
    25081 cattgagaga attagctgta cctcagcggt ttgcactgag tgggacacca atcgagaata
    25141 atttagaaga attgtggtca ttgtttgcaa cgattatgcc aggatttttc ccgactaaaa
    25201 caaaattcag ggaacttgcc gcagaagaaa ttgcgcaaat gattcgaccg tttattttac
    25261 ggcgagataa acaaactgtg ttgaaagact tgccggaaaa aacagagatg aatctatatt
    25321 ctgcacttac agaagaacaa aaaactgttt atttagctta cttgcgtcaa atgcgtgaag
    25381 aaatcacatc aatggattct gaagcattca agaaaaaccg gatcgggata cttgctggtc
    25441 tgacaagact gcgacaaatt tgctgcgatc ctcgtctgtt tattgaagat taccaaggcg
    25501 gttccggcaa gctcgaacaa gtaaaagatc tactggtagc tgccaaagag aacaaacgaa
    25561 gagttttgct gttttctcaa tttacaggga tgctgactat cttgcaagaa gagttagccg
    25621 agctagggat ctcgacattt tatttaagag gcagtacgaa accacaagat cggttaagta
    25681 tggtagacgc atttaatgct ggcgaaaaag atgtttttct gatttcctta aaggctgggg
    25741 gaactggatt gaatctcacg ggagcagata cggtcatctt gtatgacctt tggtggaatc
    25801 ctgccattga agaacaagct gctggacgtg cacatcggat cggacaaaaa aatgtggtag
    25861 aagtgtggcg tatgatctct gaaggaacga ttgaggaacg catggattca ctccagcaag
    25921 aaaaacgcga actgttccaa aaagtgatcc aaggtaatga agaacagttg acgaaactga
    25981 ctgaagaaga catccgcatg attttaagtg taggagaaat cgaggagtaa gagactcgaa
    26041 aaagcgttca gtaatgggta agaaagcctt tctaaagaag cgattgttta tttgatttaa
    26101 aaaatagcaa ggaggctggg attccatttt atcccagctt ccttgctatc atttttttat
    26161 ttctattttt aaaggttata caaagattgt cttcattgtc tctaacagct gatcaaaaga
    26221 agaaatctcg atatcgcaaa ttggagtctg ttcaattttg cgttctcgat ggttgaacca
    26281 aagcgcttgc cagttagcac tttttgcacc tagaacatca ttgtcgtaat tatcgccaac
    26341 gtataaggtc tcttctggaa gcatatgaaa ttctttctca gctagctgaa aaatctcttt
    26401 ttctggtttt tggaaacctg ttgcttgaga aatgagcata ttgcgggaag gaatccaatt
    26461 gttcaattga agttgcttca gtttttttgt ctgatggtca gtcggcccat ttgtgataat
    26521 tcctaatggg acattctttt ccttcaagaa atcgagggtt tttttgacct cctcatgcaa
    26581 gcagatatta tcaagttctt cctcgtaaat tttttgaaat aagaggccat cttcttccgt
    26641 aatatgaggg tagtctaaat cttttagtga ttggctgatc cgatgtgctc gcatatattc
    26701 tagtgtccaa tctcctgcca ttacttttgg gaagttttca tcactatgat ggcggaaccg
    26761 gatatatagt ggatgcatat ctgcatctga aacaagcgga acgactcttt tgaccgcgtt
    26821 tcgaaatggc tgctgctgat catacatcgt atcatcaaca tcaaacacaa ctgatttaat
    26881 caaaatttta cacgcctttc ggttttcatt gattttttca caaatccttg actattgtaa
    26941 cagaaaaaat tgtctaaaaa agctgataaa taaaggtttt tcttactata tttctatttc
    27001 cttgacagaa taaggttttt atatttcagt ttttttcact aaaaatcgtg aaacacgtca
    27061 ataagtttga tgaacaaatt aatcattatg gtagaagtcg attgatttat tttaatgcaa
    27121 tctctgtgga attttcgaaa gatttatttt tttaatacag tctcaatata actattcaaa
    27181 cataaaataa cgttatttga tttatttgtt tatagtaaat tttatttagt aatcattgct
    27241 ttaaaatact acgcttatat aacagagaaa ataagcgtta taataaagaa cagtattatt
    27301 taaaatgaca gaaaacatta ctattaaaga aataaaaaac ataaaaaatc ttaagaaatc
    27361 ggtttcattt agcttttgag ataaaataat agtacagttt tatacgctgt ttcatatgtt
    27421 tagaaaattt gaattagatt tgaaaactct aatttttaaa tgataaaaaa agcgagaatg
    27481 ctggttttac tgtatattaa ccttagataa ctttcatttt tttaaaaaaa ttatgaaaaa
    27541 aaggtagcta tttaaaagtt gattatgtat aatagaaaca gatgggagat tattatgatc
    27601 ttctgttaca aaaaagtgat tcttatcacg aaaaggagtg gtgcacgttg ttaacattat
    27661 atacttcccc aagttgtact tcctgcagaa aggctcgtgc gtggttgcaa gaacatcaga
    27721 ttccatttgt tgaacgtaat attttttcag aaccattgaa cagttctgaa ttaaaagcga
    27781 ttttacaaat gacagaagac ggaacagaag aaatcatctc gacacgttcc aaagtattcc
    27841 aaaaactgaa catggattta gatgatctgc cgttacaaga attattggaa cttgtgcaaa
    27901 acaatcctgg attgcttcgc cgtccaatca tgattgatga taaacgtctg caagttggtt
    27961 ttaatgaaga tgaaattcgt cggttcctac cgcgcgatgt acgacaatta gaattacgtc
    28021 aagcacaatt gatggctgga ttatagaagg ataatacgaa acttggaaaa gactattaca
    28081 aagatagttt ttccaagttt ttttgttttt acaaaaagta ttcttgatta gatgtataaa
    28141 aaggtagtag aagatgatga ttgcacgaaa aatagagagg gaaaattagc tgaaaagtcg
    28201 gctatatagt attcgtctgc caaaaatgtt atagttaaat aagatagctt gaaaggttga
    28261 aaaatccgct ggttttatta aattcgctta acagcgagat tttttttctt tacatttaag
    28321 ctgatttcgc tacaatgaat ctatattaaa cattgtggca aagtgaggtg tagcaaaatg
    28381 gaaatggaac atattaatga aaatacgatc cgtgtactaa ttggtcatga agatttagag
    28441 gaacggggcg tttcattttt ggatttactt ggcaatcaca aagaaatcga aaatttcttt
    28501 tatagtattt tggaagaagt tgatatcgaa gaggagttcc gaggaagcga agctgtcact
    28561 ttccaagttt tgccaaaagg agacggatta gaactgttta tcagtaaaaa tcttccggat
    28621 gaagaaattt ctcaatcaga agattctact gttgattcag aagatgtgag tgactttctt
    28681 cgtcagcaga ttattggcag cgatccggag gaagaattat ctggaagcgc cctagaagaa
    28741 caggaaagac aagcaacttt tgcttttgaa gattttgaac aagtgatcca acttgcaaca
    28801 gatgtcgaac tagagtctgc ttggactgat ttgtatttat tcgaagagcg ctattaccta
    28861 gtcgttcact tctggatgga aaatctcaac caagcggatg tagagaatca gattgctcga
    28921 attttggagt ttgctgaaaa gagcgatcgt acaccagaaa cattgaatga atatggacaa
    28981 tgtctgatgg cgcgtaatgc tatcgagcga acgaaatttt attttgtata aatcagcatt
    29041 atagtaagag actgtgacaa aaattgtgtc gcagtctttt tttcgtataa atgtgttcaa
    29101 aagcttgcat ctgcggtaac aagcccaacc aaacgaaaaa tattgggaac tatttttgtt
    29161 tagctgttct tattacttga tgcagaatag ctttttcacg ccctcttttt tcgtgtaaat
    29221 aaattcttag acgtaaataa gttcagaggg agaaaaatgc tgcttgcaat gaatgaatat
    29281 aataagtgct gttttgctaa acatgcagaa aaaggaaaga aatattattg tcgtggctgt
    29341 cgaaaaagag tgattttgaa aaagggaaag aaaaaatgtg ctcattttgc ccatcaaaaa
    29401 tcagataact gttcggtctt tagtgaaaga gaatcaaaag agcacttaca gttaaaggaa
    29461 tgcttcatgg attggctggg acagtcagta gaaccagtat ttttggaagc ctatttgccg
    29521 aggttgcggc aaaggccgga tattttgttg gcgaatctag caatagaaat ccaatgttct
    29581 agactgtctc accagagatt tattgaaaga acccaaaatt atttgaataa cagttaccaa
    29641 gtatggtgga tacttggtaa ttcttttctt ggacaatcgc aattctcatt gatagaaaaa
    29701 agttgctgct actataatag aaaaagagga gtgcattgct ggaaagctga tctcaagaaa
    29761 caaaagcttt atttgtatca tcatattaca gagactgtat cagggcatat ttccttttgc
    29821 agtagctgct ggactttttc tagtagggac cttaaagaaa tattcactag taacgaaatt
    29881 aaaatgaacc aaatgaaaaa aatagaaaaa gtgagtgaag atgggaagaa atggctgaca
    29941 cgccaactta ttcacaagca aaagaataca gtatccatac aagaacaatg ctacttgcgt
    30001 cataaacatc ttttgcatct atcatcgtgg atctatcaaa aaagtcggtt tttcttttat
    30061 ttacaagaac aggtttttct ttatcgaatg ttgtacgaag aagccttgga aaaccagaaa
    30121 gttcctgatt tcaactcatg gctttgccaa ataaaagaac ataaaaggga atggcttttt
    30181 cctatgattg atgaagagat ggtctatcag cagtttttta acgaatgcat acacttgtct
    30241 tctttgaaaa aatagtaagt ataaaaatag gctaggaatc ggttttctat gtaaaacaag
    30301 atttagttat gctaaaattg atggaagcag acacatgaat gagtagaaaa gttagatgct
    30361 gtgacatgtg gatgttagac tatgatctat tttgatagat agagctaagg gaagaaaagg
    30421 agtggatttc atggaagtaa agcagttgcc aaaacgagaa gaattgcctg aaaatttaac
    30481 ttgggacttg accaagatct tttcaagcga ccaagagttt gatgagaaat atttggaatt
    30541 atcagaagag ttaaaacaat ctgaaaaaca caaaggaaca cttgatcaag gcgcttctca
    30601 atttttaaat gcgattgaat tcgtattgag ggtttatcgc caaactgaag tcatttatgt
    30661 atatgcgcac cttaaaaacg atcaagacac tggaaataca gattaccaag cgctttatgc
    30721 aagagcaagc agtctgtttt cgaaagttag tgaagccgtt tcctggtttg aaccagaaat
    30781 attgcaattg tcagatgacc agatttggca atatttcaaa gaagaaccaa aattggaagt
    30841 ctatcgccat tatatccagc aaatagtaga taatcgagcc catgtcttat ctgctgagca
    30901 ggaatctctt cttgctggag caggtgaaat ctttgatgct tcaagtgata catttgctgt
    30961 tttgaataat gcagatctag tttttccaac gattgaagga gaaaatggtg aaatagtcca
    31021 attatctcat ggcgtgtatg gtcagttgct agaaagcacg gatcgaaggg tgcgcgaagc
    31081 agcatttaag gggttgtaca gtgtttacga acaatttaga aatacatttg cttctacttt
    31141 aggcacacat ataaaaggac ataattttaa agcgaaagtc cgtaattaca gctctgccag
    31201 agaagcgtct ttgagcaata atcatattcc tgaaagtgta tacgatactt tggtagacgt
    31261 ggtaaacaag catttgcctt tgttacatcg atacatggaa ttacggaaac gtttattaga
    31321 agtggaaaaa ctgcacatgt atgatcttta tacaccggtc ttaggggaag ctccaattac
    31381 ctttacgtac gaagaagcaa aagaaaaagc tttagaagca ctgaaaccaa tgggtgaaga
    31441 atacatggcc atcgtagaaa aagcattctc tgaacgttgg atcgatgttg tcgaaaataa
    31501 agggaaacga agcggtgctt attcttcggg aagctatgac acaaatccat atattttatt
    31561 gaattggcat gatacgctgg atcagctatt tacgcttgtc cacgaaatgg gacatagtgt
    31621 tcatagttat ttcactcgtt cgaaccagcc ttatgtgtac ggcgactact ccatcttttt
    31681 agcagaaatt gcttcgacaa cgaatgaaaa tatcctaacg gagtatttat tggaaacaga
    31741 aaaagaccct cgtgtacggg cttatgtact caaccattac ttggatgggt ttaaaggaac
    31801 agttttccgc cagacacaat tcgctgaatt cgagcatttc atgcatacgg aagatgaaaa
    31861 aggtgtgcca ttgactagtg aatacctaag tgatagttat ggtaaattga atgcaaaata
    31921 ttatggtcca gcagtcgaag aagacccaga aattaaattc gaatggtcac ggataccgca
    31981 tttttattat aattactatg ttttccaata ctcgactggc ttttctgctg cttcagcact
    32041 ggcgaagaaa atactaaacc aggaaccaga ggcactagag aactacttgg cttacctaaa
    32101 agcaggtaac agcgattatc ctgtggaagt aatgaaaaaa gcaggagttg atatgacaca
    32161 ggctgcatat attgaagatg caatgtcgat gtttgaacaa cgtctaaatg aattagaaga
    32221 attgatcgat cgtttataag gctttctatt tttgattttc tcgaatagaa acgaaaaaaa
    32281 tacgtcggct ctcttgtaag ggagtcgacg tattttttt
//
LOCUS       DAYLZO010000016        32223 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.016, whole genome shotgun sequence.
ACCESSION   DAYLZO010000016 DAYLZO010000000
VERSION     DAYLZO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.016"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..1663)
                     /gene="ptsP"
                     /locus_tag="ACGVJG_000721"
     CDS             complement(<1..1663)
                     /gene="ptsP"
                     /locus_tag="ACGVJG_000721"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFJ6556699.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELAD"
     gene            complement(1663..1929)
                     /locus_tag="ACGVJG_000722"
     CDS             complement(1663..1929)
                     /locus_tag="ACGVJG_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFJ6556700.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            2408..4642
                     /locus_tag="ACGVJG_000723"
     CDS             2408..4642
                     /locus_tag="ACGVJG_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6556701.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            4790..5110
                     /locus_tag="ACGVJG_000724"
     CDS             4790..5110
                     /locus_tag="ACGVJG_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFJ6556702.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5315..5539)
                     /locus_tag="ACGVJG_000725"
     CDS             complement(5315..5539)
                     /locus_tag="ACGVJG_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFJ6556703.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(5737..7317)
                     /locus_tag="ACGVJG_000726"
     CDS             complement(5737..7317)
                     /locus_tag="ACGVJG_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFJ6556704.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(7718..9094)
                     /locus_tag="ACGVJG_000727"
     CDS             complement(7718..9094)
                     /locus_tag="ACGVJG_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFJ6556705.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(9222..10310)
                     /locus_tag="ACGVJG_000728"
     CDS             complement(9222..10310)
                     /locus_tag="ACGVJG_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFJ6556706.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            10482..10916
                     /locus_tag="ACGVJG_000729"
     CDS             10482..10916
                     /locus_tag="ACGVJG_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6556707.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(10994..11527)
                     /locus_tag="ACGVJG_000730"
     CDS             complement(10994..11527)
                     /locus_tag="ACGVJG_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFJ6556708.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(11621..12799)
                     /gene="mutY"
                     /locus_tag="ACGVJG_000731"
     CDS             complement(11621..12799)
                     /gene="mutY"
                     /locus_tag="ACGVJG_000731"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFJ6556709.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(12792..13589)
                     /gene="recX"
                     /locus_tag="ACGVJG_000732"
     CDS             complement(12792..13589)
                     /gene="recX"
                     /locus_tag="ACGVJG_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFJ6556710.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            13696..15078
                     /gene="rlmD"
                     /locus_tag="ACGVJG_000733"
     CDS             13696..15078
                     /gene="rlmD"
                     /locus_tag="ACGVJG_000733"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFJ6556711.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(15252..15830)
                     /locus_tag="ACGVJG_000734"
     CDS             complement(15252..15830)
                     /locus_tag="ACGVJG_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFJ6556712.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            16152..16622
                     /locus_tag="ACGVJG_000735"
     CDS             16152..16622
                     /locus_tag="ACGVJG_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFJ6556713.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(16730..17956)
                     /locus_tag="ACGVJG_000736"
     CDS             complement(16730..17956)
                     /locus_tag="ACGVJG_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFJ6556714.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(18710..18910)
                     /locus_tag="ACGVJG_000737"
     CDS             complement(18710..18910)
                     /locus_tag="ACGVJG_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFJ6556715.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(19044..19652)
                     /locus_tag="ACGVJG_000738"
     CDS             complement(19044..19652)
                     /locus_tag="ACGVJG_000738"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFJ6556716.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(19737..20207)
                     /locus_tag="ACGVJG_000739"
     CDS             complement(19737..20207)
                     /locus_tag="ACGVJG_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFJ6556717.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(20255..21169)
                     /locus_tag="ACGVJG_000740"
     CDS             complement(20255..21169)
                     /locus_tag="ACGVJG_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFJ6556718.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            21315..22118
                     /locus_tag="ACGVJG_000741"
     CDS             21315..22118
                     /locus_tag="ACGVJG_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFJ6556719.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            22296..23243
                     /locus_tag="ACGVJG_000742"
     CDS             22296..23243
                     /locus_tag="ACGVJG_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFJ6556720.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(23311..23604)
                     /locus_tag="ACGVJG_000743"
     CDS             complement(23311..23604)
                     /locus_tag="ACGVJG_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFJ6556721.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(23632..24297)
                     /locus_tag="ACGVJG_000744"
     CDS             complement(23632..24297)
                     /locus_tag="ACGVJG_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFJ6556722.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(24437..25069)
                     /locus_tag="ACGVJG_000745"
     CDS             complement(24437..25069)
                     /locus_tag="ACGVJG_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFJ6556723.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(25076..26143)
                     /gene="liaS"
                     /locus_tag="ACGVJG_000746"
     CDS             complement(25076..26143)
                     /gene="liaS"
                     /locus_tag="ACGVJG_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFJ6556724.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(26140..26871)
                     /gene="liaF"
                     /locus_tag="ACGVJG_000747"
     CDS             complement(26140..26871)
                     /gene="liaF"
                     /locus_tag="ACGVJG_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFJ6556725.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(27040..27519)
                     /gene="greA"
                     /locus_tag="ACGVJG_000748"
     CDS             complement(27040..27519)
                     /gene="greA"
                     /locus_tag="ACGVJG_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFJ6556726.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(27655..28830)
                     /gene="mltG"
                     /locus_tag="ACGVJG_000749"
     CDS             complement(27655..28830)
                     /gene="mltG"
                     /locus_tag="ACGVJG_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFJ6556727.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            29034..30203
                     /locus_tag="ACGVJG_000750"
     CDS             29034..30203
                     /locus_tag="ACGVJG_000750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFJ6556728.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(30470..32104)
                     /locus_tag="ACGVJG_000751"
     CDS             complement(30470..32104)
                     /locus_tag="ACGVJG_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFJ6556729.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         9896 a         6365 c         5268 g        10694 t
ORIGIN      
        1 gatcagcaag ttcagccatt ttcgctgtgt ccaagcgttt cattaaacta cgcgttttaa
       61 gaatagatgt cgcactcatt gagaactcat ctagacccat acctactagc aatggaacag
      121 ctgtttgatc tccagccatt tctccacaca tacctgccca ttttccttca gcgtgtgctg
      181 catcaattac atttttgatc aaacgtaaaa ttgatgggtt atatggttgg tacaagtaag
      241 aaacgcgttc gttcatacgg tctgctgcca ttgtgtattg aatcaaatcg ttcgtaccta
      301 cagagaagaa atctacttct tttgcaaact tgtctgctaa gactgctgct gctggaatct
      361 cgatcatgat gccgacttgg atcgtatcag atacttcctt gccttcactt atcaattttt
      421 gtttttcttc ttcaaagatc gcttttgctg cgcggaattc tttgagtgta gctaccatag
      481 ggaacatgat acgtaaatta ccatgaacag atgcacgaag caatgcacgc atttgtgtac
      541 ggaacatttc gtcgccttgt tcagataaac tgatacgcaa tgcacgatat cctaagaatg
      601 gattcatttc atgaggtaat tgcagatatg gtagttcttt atctccaccg atgtccattg
      661 tacgaacaac tacaggtttt ccttccatac cttctaatac agctttgtaa gcttcatatt
      721 ggtcatcttc tgttgggaag tctggagaat ccatatacag gaattcagta cggtacagac
      781 caactgcttc tccaccatta ttatggacac caactagatc ttttggtgtt ccgatgtttg
      841 cagctaattc aaaatgcttg ccgtcagctg tgacagtttc tgcatttttt aatttttccc
      901 attctgcttt ttgtgctgcg taatctgcac ctgctttttc aaattcagct ttttgttcat
      961 ctgtaggatc gatgattaca tcgccttcga tcccgttgac agccaaaata tcgccttctt
     1021 tgactttagc agtaatttct tttgtcccaa cgattgctgg aatttctaaa gaacgtgcca
     1081 tgatagcaga atgtgaagta cgtccaccaa tgtctgttac aaacgctttt acaaaattgc
     1141 gatctaattg tgctgtatca cttggagtca agtcatgtgc aacaacgacc acttcttcgt
     1201 tgatcattga tggatttggt aaagtaacgc ctaataagtg cgctaaaata cgtttagcaa
     1261 cgtcacgaat atctgcagca cgctcttgca tataagcatt atcttccatt gcttcgaaca
     1321 taccgatata catatccgtt acttctttta gcgctgattc agcatttacg ctgttgtctt
     1381 taatattttg cttgatttga cctaccattt ctggatctga aagaaccatc aaatgggcat
     1441 caaatacttg cgcttctgct tcacctaagc tttgcgctgc tttttcacga atttgctgca
     1501 attcttcagt agatttagct aatgcagcat ccaaacgagc ttcttcagcc gcagtatctt
     1561 ctactgttgc cttactgaat gttaaatccg gttgaactag caggtaagct tttgcaacgg
     1621 ctacaccgtc actagcggca atccctttta gcatttcaac cattattcag ataagccttc
     1681 tttcttcatt gtttcaacga ttgctgccat tgcatcagct tcgtcagcac cttctgcagt
     1741 aatagtaaca tcagaacctt gacctacacc taaagacata acgcccatga ttgatttcaa
     1801 gtttactgat ttacctttat actctaagtt tacatcagag ttaaatttgc ttgctgtttg
     1861 tactaatagt gtagctggac gagcgtggat cccagtttct gctactacgt gaaattcttt
     1921 cttttccata ttaatcggtc tcctttaagt gagtgttgtt tttatgtgag ggttttcttt
     1981 tttagtaatt cagagaatcc taacaaatgc caccctttca tctgtaaaga ttaacatttt
     2041 ttttcgttag atacaacagt tttcattcaa tttcagataa aagtattcaa attattccgg
     2101 attctttttc atccttttct tcacatttta taacaaacgt gtttatttcc tttttcttgt
     2161 cgctttctct tctcctgacg tttatcattc tttcctttga ccctctttgt tttcatatga
     2221 aacgaattac ttgcttccct ttttattttt agtataataa gagtcaaagg tcaggaatgg
     2281 tcaatagaga tttcaccttt tctacctcat aaatctctta aaaaactagg ggttattctt
     2341 ttacctcatg aaaaacgaga tgtaaaatat ggatgtaccc atttttgtga aaggacgtga
     2401 atgattcatg ctttgtcaaa actgcggcaa gaatgaggca acgattcact tgtatgccac
     2461 tgtcaatggt cagcggacac aactcgatta ctgtcagagc tgttatcaaa aaatcaaaaa
     2521 tcaacaaaat ggaggtctga ctaatatggc ccaaaatgat ccatttggat ttggcagttt
     2581 ggacgatctt ttccgttcca tgtctcgcca aatgcaacag caaggaaatt atgagcaaac
     2641 accaccaaca caatttggcg gaaacaacaa cttcaatggt ggtcagccac ctcaaggcaa
     2701 tacagaaggt ttattgggag aatacggaat caatatcaca gaacaagcac gacaaggtga
     2761 tatcgatcct gtgatcggac gcgatgacga aatcaaacga gtcattgaaa ttttaaatcg
     2821 ccgaaccaag aacaatcctg tcttgattgg agaaccaggt gttggtaaaa cagcagttgt
     2881 tgaaggttta gcacaaaaaa tcgtagatgg tgatgtaccg caaaaattaa tggacaaaga
     2941 agtcattcgt ttagatgtcg tatctttagt ccaaggtact ggtatacgag gacaatttga
     3001 agaacgtatg caaaaattga ttgaagagat ccgtcaagca gaaaatgtga tcctattcat
     3061 agatgaagtt cacgaaatcg tcggtgctgg ttctgctggt gatggaaata tggatgctgg
     3121 taatatttta aaaccagctt tagctcgagg agaattgcaa atggttggtg ctactacctt
     3181 aaatgagtac cgcatcatcg aaaaagacgc tgctttagaa cgtcgtatgc aacctgtacg
     3241 ggtagatgaa ccaactgtcg aagaaacgat cagtattttg aaaggtttgc aaaaacgtta
     3301 tgaagactat catcatgtaa aatatactga tgaggccatc gaagcagccg caactttatc
     3361 caatcgttac gtccaagatc gcttcttgcc ggataaagcc atcgatttgc tagatgaaac
     3421 aggttctaag aaaaacttga cgatccaaat cgttgatcct aaaacaatcg agaaaaaact
     3481 gcaagaagca gaagaacaaa aagttttagc ttctcgagaa gaagatttcg aaaaagcagc
     3541 ttattatcgc gatcagatca ataaacttca aaaaatgaaa gaacgtcagc tcactgaaga
     3601 ggaaacacct gtgattaccg aaaaagatat ggaaaaaatc gttgaacaac ggacaggtat
     3661 tccagtgggt gaattaaaag aaaaagaaca gacacaactg aaaaacttag cagatgactt
     3721 gaaagctcat gtcatcggac aagataacgc tgtagatcga gtagcgaaag cgattcgccg
     3781 aaaccgtgtt ggattaaaca aacagaatcg tccaatcggc tccttcttat ttgttggacc
     3841 aactggtgta ggtaaaacag agcttgcgaa acaacttgcc tacgaactat tcggttcaca
     3901 agattcaatg attcgtttcg atatgtctga atatatggaa aaacatagtg tctctaaatt
     3961 gattggttct cctccaggtt atgtcggata cgaagaagct ggtcaattaa cagaaaaagt
     4021 acggagaaat ccttacagtc ttgtcttgtt agatgaagta gaaaaagctc atccagatgt
     4081 tttgcatatg ttcttgcaaa tcttagatga tggtcgattg acagatgcac aaggaagaac
     4141 ggtcagcttt aaagatacga tcatcattat gacaagtaat gccggaactg gtaaagtaga
     4201 agcaaacgtt ggttttggtg cggcacgcga aggtgttact cgttctgtat tgaaccaatt
     4261 aaacaactac tttacaccag aatttctgaa ccgttttgac gggatcatcg aattcagtgc
     4321 attatcaaaa gaaaacttga tgacaatcgt cacactaatg ctagacgatg tcaaccaaat
     4381 gctggcagca cagcaacttc acatcgaagt tcctacgaat gtcaaagaaa aattagttga
     4441 cttaggctat gatccatcta tgggtgctcg tcctcttcga cgcacgatcc aagaacaaat
     4501 cgaagatggt atcgctgaat tttatcttga tcacccttct atccatgagt tgaaagcaaa
     4561 actggataaa gatggaaaaa tcatcgttac ttctaaacca gaacgtttgg ctaaagaatc
     4621 agccgaagaa acggctgaat aaatccagtt ttatagcgtg agacggtatt tctttgagaa
     4681 atgccgtctc attctttttt cagaaaagtc aaaatactgt tcattttata aaaaagaaat
     4741 tataataaga gaaacaagca attaaaaata cataaaagag gtgagcgcca tgaaattggt
     4801 aaatgtaaca aacagccatt cccgtcttgt attaaaccag cttgaaaaca ctgatgccca
     4861 tctagtcaag gtctacacag ccggaaatac cactattgtc tacactgaag cccctgaaca
     4921 taatgaaatt ctgctgatca atgacaaacg taaaatccag ccgaaagaaa tcgaagatgc
     4981 taaaaattat ttcttagata aaatcaaaga cgccgtttac cacgaggatg aaatcaaagt
     5041 aatcgaattg gcaggccttg ttgaaatctc gattccaaaa catgaattag gcgctacatt
     5101 agccgtgtaa acaaattttg aacataataa ttaaagagcg tgggacaaaa gtaaagaaca
     5161 cttttgtctc acgctctaaa cacgtataaa cggcgaaagc agaagcaact ccttcggaaa
     5221 taagctgaaa ttcacgaaaa ttgcttttca aatttttgtg aattccagct tatttcttgg
     5281 agttaaacgc ttctgtctca gcctcttttt ctttctagct ttttgatttt tctcttttct
     5341 tttgtacttt tctcttttca ttttcagttc tacgcgtgtc tttattttta actttttcct
     5401 ctttatggat gattgatcgt actttttgtg caatttgctc gtttttgtcc cagtctttta
     5461 catctaataa tcgacataat cgtaacatct cttctttttt ctgttttttt ctttcacgtc
     5521 tttgccattc tgtttccatg tgtcacactc tcctttcttt aatcatatgt acgcaaaata
     5581 atttttatat ttttttgatt agacatttct atgattttaa tatagtaaaa agagccgtca
     5641 cggaaaatcc gtcacggctc taaacaaaat atgagaggct gtttttgttt ggctattctt
     5701 attacttgat gcagggcagc ttgtgtcaag acttctttac atcaaacttt ttaattttac
     5761 attgggatat ttatccgcaa accagcgttc tgcaaattgg ttctcgaata agaacaacgg
     5821 ttggtcgaaa cggtcacgag ccaagatatt gcgacttgaa ctcatcttct catccaaatc
     5881 ttctggatcg atccagcgag cgattttgtg tcccattgga gacatgatta cttcggcgtt
     5941 atactcattt aacatacgat attggaatac ttcgaattgt agctgaccaa cagctccaat
     6001 gatatattct tccgttagat atgttttata aagctgaatc gctccttctt gaactaattg
     6061 atagattcct ttgtggaagg atttttgttt catgacattt tttgcagtca ctttcataaa
     6121 cagctctgga gtaaatgaag ggagttcttc ataagcaact tttagttttc cttcgaacaa
     6181 tgtatcccca atctgataat tgcctgtatc atatacaccg atgatatcgc ctgctactgc
     6241 tttttcgacg ttttctctcg aatctgccat aaattgtgtg acattgctta atttcagttt
     6301 tttatttgta cgttccaacc agacatccat tcctcgttcg aatgttccag aacagattcg
     6361 gacaaaagcg attcggtcac gatgggctgg attcatattt gcttggatct tgaatacgaa
     6421 acctgaaaat tcttcctcat aaggacttac catctgatca tcttgtgttt tgtgtccata
     6481 aggagctggt gccaattcaa cgaaagtctc taaaaaagtc tgtacaccga agttagtcaa
     6541 tgcagaacca aaaaatactg gtgtctgatc gccacgagca attttttctt gattaaatgc
     6601 atctccagcc tctttgatta gttctacttc ttctaagact tgttcatata aggaattttc
     6661 agttagagga tgattttcgg gaagcagtcc atcttcatcc aattctacta atcgttcatc
     6721 ctgataattt tccggacgat aaaactctac acgattatgg tagatatcat aaagaccttc
     6781 tagcccttta cccataccga ttggccaatt catcggataa gattcgatat ctaatagctc
     6841 ttctaattct tctaacaatt ccaatggttc acgaccatca cgatctagtt tgttgataaa
     6901 agtaaatatc gggatgccac gtttttttac aacttggaat aatttctttg tctgtgcttc
     6961 gatccctttt gcactatcga ttaccatgac cgcactatcc acggccatca aagtacgata
     7021 agtatcttca gaaaaatctt catgtcctgg tgtatccaag atatttactc gtttcccttg
     7081 ataatcaaac tgcataacag aactagtaac agaaatccca cgttgtttct cgatttccat
     7141 ccagtctgat ttggcaaaat tacctgtttt tttgcctttt acggtcccgg cttgacggat
     7201 agccccacca aacaatagta attgttctgt aatcgttgtt ttcccagcat ccggatggga
     7261 aatgatcgca aaagttctgc gattgtccac ttgttgcttt aattttggat tattcataac
     7321 ctaactccat ttcttccatt caattattta tattttctct aggcacagat tgtccgatct
     7381 cttaaagaaa gacaatttca tatagggctg tgacaaaagc cgtgtagcag tctctttttc
     7441 cgagtaaacg gcgttctaga gacagcctat cagcaataag cccacccaag ccaaaaatac
     7501 gaaaagctgt ttttgcttgg ctgctcttat tacttaatgc aggatagctt tttcacaacc
     7561 tttgtcaact actacaactg aatcagtata gcatggattc agcgaatgaa aaagccacca
     7621 aaccataaga gtcaaatatt tttttgtcag ctgactaaaa gaaaagacgt gttgcttgtt
     7681 tattcaagca acacgtcttt cataaccaag tttccaatta tcttcttggc tcgtcatctt
     7741 cgaattcttt tctgaagtat cttcgttctt cttcaggttc cgcttctgtt tcttccggat
     7801 catgaaaaat gacacggatt ttaaggattc gagaaccttc catttcttca gaaaccagcg
     7861 taatattatc gacatcaaac gataactttt cgccttcatc cggaatcatg cctaatgctg
     7921 taatcaaata accagccatc gtatctacat cactcatatg cagatcactg tcaaaagctt
     7981 cattgaactc atcaatcagc atacgtcctt ggatgatata ttcgtgttca tcgatttttt
     8041 cataaagatt ctcaacttca tctgattcat catcgatttc tccgacgatt tcttctaaaa
     8101 ggtcttctag tgttgctaac ccaacgacgc cgccatattc atctaataaa atagccatct
     8161 gattctgcgt ctttttcatt tcatataaca gatcatcaat aaagaccgtt tcaggaacga
     8221 ataaaggctc ctgcatgatt ttcttgatat ctaaattatc aaagccgaat ttatgggcag
     8281 cttttagtaa attctttgta tgaaggattc cgactacttt atctttgtct tcgttataaa
     8341 cagggattct tgaatagttt tcggataaaa cttcattcac attttcttcc acagtgtcgt
     8401 tgatatcaat cataaatgca tccgttcgtg ggaccatgac ttctcgggca actttcgtat
     8461 caagagaaaa aaccccttgg agcatttcta attcttcatt attcaatacg ccttcgtttt
     8521 caagcatata acgcatttca tctctggtca tttttgaatc ttcatcatca aaagtcatcg
     8581 gcgtgatacg cgccaataaa cttgtggaag cagaaagcag ccagacgaag ggcttagcta
     8641 ctacaccaat caagcgtatc agccctgaag tgaactttgc gacttcttct gatttattta
     8701 aagcaatacg tttagggtac aattctccaa aaacaatcga tacataggtc aaaatagcca
     8761 gcacaatgat atttgcaata ttttttgccg ctgtgccgcc acctaatacc ggagctaacc
     8821 gttccgataa agtattagct agtgaagcac cagacaaaat gttgaccaaa gtgattccta
     8881 cttggattgt cgataggaaa ttatttggat tctgcaatat ttttaaaagc ttttgagatt
     8941 tcacatctcc ttcatcagct ttttgctcga ttcgattctt attgatcgaa acaacagcaa
     9001 tttcggaagc tgctaaaaaa gcattgatca atgtcaaaac gatcaataat aaaatctgcg
     9061 ctaacagcga ctgactctca gggtcagcat tcataatcgc attctctcct tataaaatta
     9121 atttttagtt tacgatagaa aaaaacgtgg cactatagcc acgttaaaat ggtatcacaa
     9181 agcaattttc tttgcaataa aaacaagtgt actaaaacta cctacgaggt cgtagcaatt
     9241 ttcttcttgt tttcattttg cttgtcttct ttgacgattt tgattatata ggaaatgatc
     9301 gtcttgcaga ttgcgtaaaa aggaacacct aagaagatac caagcaatcc ggcaatattt
     9361 ccagctacta gaagaacgat aatgatcgtt aatggatgga ttttcagtga tttcccaatt
     9421 acattaggat aaatgatatt cccatccaac tgttggacga ttagcacaac cacacaacaa
     9481 agtaatgctt ttaccggatc attgaagact gttactagaa atgcaggtgc tagtcctaga
     9541 taaggtccta aatatggaat taggtttgtc aatcccgcaa tgaccccaaa taagaaagca
     9601 taatccacac caattaccat atatcctaaa aatgtgaagg taccaacgaa taaacattcg
     9661 attgcttgtc cgctaatgta attagccaat gttttattca gttgccccaa taactccaca
     9721 atttggtttc ttcttttttc tgggaaaaac tgttggatgt ttggcactag tttttctccg
     9781 tctttcaaca tatagaacaa gatgaatggt gctgtaatga ttactacggt tgccgaagcg
     9841 atcgttccaa cgatcgaacc taaactattg gatactccac taaggaattg ctgaatgatt
     9901 gtcccgtaag aaatgtctaa ttgctcaaaa tatttattga tatctacttc cttgaacaaa
     9961 ggcaattcgg ctacctcttt taaccagctt tctacctgtt tgatgaaatc aggcatatta
    10021 gatgccaatg aggaaatttg ctgtactaga cttgggatga cactaagtaa aatccaaata
    10081 attgctgcaa ctaaaaggag cagcacaatt gcgacagccc atattctttt gatttttgct
    10141 ttcatcaaca gcttgacaat aggattcaat aaatagtaaa gaaagcctgc aatcagtaca
    10201 ggtgcaaata acgtggaaaa aaatgtcccg atcggttcga aaataaagtt tatttctgat
    10261 gaaatcatga tcaatgcagc tatgatcaat aattcaagcg accaaaacat tagtttagac
    10321 tgtctaagtt tttcaaacaa tagatttcct cctttctttc ttactagtat atattatatt
    10381 cgttaaaaat tcttttattc tttttttatt tttgaaaact tgccatactt tttatttgtt
    10441 ttattttcca gtatactata cgaaaggaag gtggctagac catgaaagtt gtacaaacaa
    10501 aagatacaat gagcaatatt tacttagatg ctgtacggat caggcatcaa gtttttgtag
    10561 tcgaacaggg tgtgcctttg tctcgagaaa tagacaaaga tgaagcccat tgtatccact
    10621 tcgtcctgta ctcagataaa aaagaaccgc aaggaactgt acgtcttttg cctttagaaa
    10681 acggaaaaat gaaattacag cggatggcga ttctttctga atacagacac caagggttag
    10741 gaaaaatatt gattgaagaa gcagaaaact ttgctaagaa tcaaggctat aatacgattt
    10801 tattaggggc acagtccaca gctgaaactt tttacgaaaa actaggatat acagcttacg
    10861 gcgatccttt tgaagatgct ggcatgcctc atatttatat gaaaaagact ttataaaaat
    10921 ttatttcgaa aaaagcaaaa aacagcaagc ttttgagaaa aatctctcac tggcttactg
    10981 ttcttttttg tgtttactta cgcgacaatt gcttgtaacg gttataccaa atatcaatat
    11041 agccttccga gaacgggcca tctccattat tgatccagtc aaccagaatc ttcacatttt
    11101 cttttaggat gaagtcaata tcgttcgggt aattcatcat tttgctgtgc atctcatatt
    11161 catcgacatc caatagtcgt ttctcgccgt caggaaatac cttgatatcc aagtcatagt
    11221 cgatatattt caaggcctca tcgtccagca aaaaaggtga agctagatta caataatagg
    11281 aaaccccctt ttctctgatc attgctatta cattgaacca gtatttttta tgaaaataaa
    11341 caatcgctgg ttcgcgcgtt acccatcggc gtccatcaga ttcagtgact aatgtgtgat
    11401 cattcacgcc gatcatagag tgttcgcttg tttttaatac catggtatct cgccacgttc
    11461 gatgcaattt gccgtcgtgc ttgtaacttt ggatcgtcac aaactctcct tcttttggaa
    11521 ctcccatagc aacccttcct taagcagcat tttgtgcttt gtgtttgggt gttcattaca
    11581 ccgacatcat aattattata tcaataaaaa tgagctttgc ctagaaagga ttcctattgt
    11641 ttcgattttt ctctaactga tctaccagtt tcatttggac cttaggaaaa acgaccgaat
    11701 caaatgcatc aggcttcaac cacttcgaag ttttgttctc ggtaaattct tgttctgctc
    11761 cctcggttgc ccgtccgtaa aaaaggagaa catgccattt cagatgacta aaaatatgag
    11821 taacttctcc taaatgtcgt gtctgccaaa caaagagttc ctgcttttca aatgggagat
    11881 tttgctcttc ttctgctact aggtcatcaa acagatcgat ttctggcttt gtttcccatt
    11941 cttttttcaa tcgctcatac tcctcttgtg tcacttccat cattggaaat gtccacatat
    12001 tcgccagcaa tttttggcta tctctttctt caaaataata cgctccagaa tgattttgaa
    12061 gagctgcact gatataataa acgtcttttg gctttgcttt ttttgttttt acaggaaaac
    12121 tcgtttggat tcctcgtttg ttagctaaac aaaaggcttg gatcggacaa gtctcacact
    12181 ttggagaagt tggtgtacag atagctgaac ctagatccat cattgcttgg ttgaattcac
    12241 ctggatgctt ttcatcaatg attttccgca tcgcttcatc aaagattttc ctgctagaag
    12301 cttttgcgat gtctgcttca atgcaaaaca atctgctgac tactcgcatc acgttgccgt
    12361 caactgcagg ttctggaagt ccaaatgcga tacttgcaat cgcgcctgtc gtatatggtc
    12421 ctattccctt caatgagcta atttcttcag gcgtttgcgg catttcccca tcaaattctg
    12481 acatgatctg tttagcagca gcttggatgt tccgggctct tgagtaataa ccaaggccct
    12541 cccatgcttt taaaagcttt tcttctggag catttgctag ctcttcaatt gtaggaaacc
    12601 attccatgaa acgataaaaa taatcgatca ctgtgtctac tctggtttgc tgaagcatga
    12661 tctcagagat ccagatacga taaggatcac gattatagcg ccaaggcagg tttctttttt
    12721 cttgttcata ccattgaatg aactgatctt gaaattcttt tgtttcttta tctgtccatt
    12781 tttcccattc tttattcact ttcctcttcc ttttccgtaa tgaatcgctg gatcatatcc
    12841 aaatcaaatc ctttttgata taagctttgc tttactttta gatttctttt tttaggttca
    12901 aaccttaaat ttcttcgcca tagtttatct ccttgtttga ctaaataatc atattctatt
    12961 tcttgatctg cttcttgcgg aagatcagct atcgcttgtt ggatcacttc ttgagaaaag
    13021 cctttcgtca tcaatgtttg ttggattttt ctcaataatt ctttttggct ttttccgtga
    13081 ttttttccaa acgctttttc ggctgtctga tgtgcatttt gaacttggtc ttcaaatgta
    13141 tattggctga gcgcttgttc aatgatttca ggtttcaaac ctttttgctg catctgctgc
    13201 gctaatttct ttggtccttt gtcacttaag cgcatattcg tgcgcacata actttcccca
    13261 tagaccaaat catcgacaag gtccaattct tttaagcggg ctacgatttt atttcgatct
    13321 tcttgaagga tttccttttc ttttaagtat gttcggattt ctttttccga tcgcaactga
    13381 taacttaagt aattcattgc taggtgaaaa ccgaaatcag ctccgctact cttcttgatc
    13441 tcttggatag tttcttcgtc taattcccgt ccttttaata atcgatagcg tactaataca
    13501 tcttcagata cttgtaaagc ttctccttca gataaatcta cgcgatagaa agggcctttg
    13561 cctttactga ttctcgtaac ggttaacata aacatcctcc tcgaacatat attctctatc
    13621 ataccacaga aaatccccgt aatcaggtat tatatgttag aataaaccac agaaaaaaga
    13681 aaaagggaga tacttatggc agaaacaatt gtcaaaactg gacaaacggt tcctttgaaa
    13741 atcaagcgtt taggcataaa tggtgaagga atcggttatt tcaaacggtt gatcatattt
    13801 gttccatatg cactaccaaa agaagaagta ctagtgaaaa tcacgaaagc aactcctcgc
    13861 tatgcagaag gagaattaat aaaagtcaaa aaaacaagca aagatcgcgt agtggcacca
    13921 tgtcctgttt actatgaatg cggtggctgt caattacaac acttagctta tcaagctcaa
    13981 ttggatttta aaaaggatct gcttttgcaa gcattggaaa aattcaaacc agcaggcttt
    14041 cgatcttatc aacttttgcc gacgatcggg atggatgagc cttggcatta tagaaacaaa
    14101 gcacaatttc agttacgaaa aaataagcag actcaaaaaa ttgaagctgg tttatacgaa
    14161 gcaaactctc atcagttagt ccctataaca gactgcttgg ttcaagaacc tcgaacacaa
    14221 aaagtaatga atactgttgt tcaactattg aataaatttg atgcacccat ctatgatgaa
    14281 cggacaaaca gtggaatctt ccgcacaatc atggttagaa tcggtgttaa aactggggag
    14341 ttacaagttg tttttattac tcgcagtaaa aaattcccgc aaaaaaacgc aatgatccgt
    14401 gagatcaatg aacgactgcc cgaagttgtt tcaatcatgc aaaatgtcca ggcacaaaaa
    14461 acgtctctcg tgatggggga tgaaacgatc cacttatggg gaaaagaagc cattgaagaa
    14521 caaatcaatg aagtgacttt cgatctttct cctcgtgcct tctttcaact caatcctcag
    14581 cagaccgaat tgctttatgg cgaaggaatc aaagcattag atgtccagcc taatgaaacg
    14641 atcgtggatg cttattgcgg agttgggacg atcggcttaa gtgtagcaaa acaagccaaa
    14701 gaagtacggg gaatggatac cgttcctcaa gcaatcgctg atgcaaaaaa aaatgcggaa
    14761 agattaggat atcacaacac tcgatatgaa gtcgggacag cagagcaagt tctgcctaaa
    14821 tggttgaatg aaggttttca gcctgatggt atcatcgtag atccaccacg tacgggtttg
    14881 gataacgcat tgatccaatc attattgaaa taccctccaa aaaaacttgt ttatatctcc
    14941 tgtaatgttt ctacacttgc cagagatctg gttaatttaa gtaaaaagtt caagattgaa
    15001 tatttacagt ccgtcgacat gtttcctcag actgcacgtt gtgaagttgt cgtgaagatg
    15061 acgagactcg ctgactaaat gaaagaggtt gtgaaaaagt ctgccctgta tcaagtaata
    15121 agaacagcca aacaaaaata gctcttcatg ttttatgaat actgtacttg tgaccgaaga
    15181 gttaacagga ggacactatt tatttaaaaa aagggttgtg acatagtttt tgtcacagcc
    15241 ctttttgatt attattggat attcacgtct atcacttgat aaaatgcatt tgtcgtatcg
    15301 ctgatattcc atactgctaa aatcacatta tatccttttc gatcctgcgg aagtgtgatc
    15361 gtttggttga ctgtttttgg tggaacagtt gctttgtcgt ctatttttgt gatcaattca
    15421 aaatcactaa atttcaaagg ttgattagga ttccaagttg gtttggtaat gtagtaatcc
    15481 caagtacttg tccgatgttg tgctgttaaa ttccaagtaa tatttaatgt tcctggatta
    15541 accatgtttt tatgccatct gttagatgtc tgttcatcta aaggctcaaa gccgctgact
    15601 cctgcactag caatttttcc atcaatgaat gtatttttgg tagcttcgat actttgcggt
    15661 tcccattgtg ctcttccgac attttgattt aagtttcctg ctgctgatgt tcctaggtat
    15721 gcacggcttc ctggagaagt aatataacca tgcgcaaaac ctggaatgct aaaaccgaac
    15781 aaccctaaac taactaaccc aataaacaat aacgtactaa tctttttcat gtagaaacct
    15841 ccttcatttg ataaccacat tgtaaacgct ttattgtatt aacacaaaca ttatgctttt
    15901 ataattttat gttgaagtaa aaaaggtgaa aaaagtaaga tgcagattat aacagaagta
    15961 gacgagaaaa gtacaaatag aaaacatgag aatcaaatca tatgatcttt ttcaaaaaca
    16021 tcattcatat aaacaaatgt tcaacttttt aataacaaca attatcaatt tttttcttac
    16081 ttcacaaact aaatacaggt cttgctgtga gccttcatgg taaactaata ttatagaaag
    16141 gagttagtaa aatgaagatg ataaaagata ttcgtttttg ggtttgtgtt ataggaattg
    16201 tcatattagg ctttttatca gggttgctta gcggaaatcc cggaaaatat tactattctt
    16261 tgcaacttcc accatttgca ccacctagct ggatatttgg gccgatgtgg accttactgt
    16321 atatattgat gggtatttct ctttatttgt tacttaatca caacaacaaa aaacagcgaa
    16381 acaatcttgt cggtcttttt gtgattcagt tcatttttaa ttttatatgg tctgctctat
    16441 tttttaattt aagaaatatt ttcattgcag caatcgatat tacactttta gttattttct
    16501 tgtcagtgct tatgtatcag ttgtggctgc atcatcgcct ggcaatgtgg ctcatgattc
    16561 cttattattt atgggtacta ttcgcaactc tattgaatta ttcaatttat tttttaaact
    16621 aagcgctaaa aagagacgtc ctgtaattta acacatagaa aaagtgccac ctaagttaga
    16681 ttttttgtct aatttaggtg gcactcttta ttaaccaaac tatttttttc tagtacccac
    16741 ttgttgttcc atactcatta ttctggtcat aaccataacc attttgttcc tgtccagcgt
    16801 aaggatcata actttgagta tcttgacttg gtgtatagta ctgacttgta tcttctgttt
    16861 gctgataata ggtattcgga tcttggtact gatcatatgt gttttctgaa taggaagaat
    16921 aatcggtgta cccttcacta ttcgatccat caccgtttgt cgaatcgcca tcttccgaat
    16981 catcaaagaa catcattgaa gcattctcat ttttcaaatt aggtaatgta tcgcttgttt
    17041 ttatatttaa ttgtttcttc agttcatttt ggatcgaaag aagatcattt tttcctaaaa
    17101 tttgatagta gatatcattg atcatttgtc cttcgccttg caactggtct tgtttgattg
    17161 tttcaaacgc cggggtgtag ttcgtcgcta catcaagcat gtcatcccaa tctaagtccg
    17221 tcgtcacatt gttctcaact gcttttaaaa tttttctata attgcttacg ccatctaaac
    17281 ttaaaacttt attgacgatt ttcgtcacta cttcccgctg acgacgttgt cgaccgatat
    17341 cgccttctgg atcttcgtgt cgcattcgag aataggctaa acctttttct ccattcaatg
    17401 tttgttcacc tactggtaca tggacaccat ccaaagtaaa gtcaatttta ttattcactt
    17461 tgatgccacc gaccgcattg atcaaatctt ccatgccatc caaattgatc gttacataat
    17521 gatcaatagg aatatcgagc agttgctcaa ttgaatccat ggccatttcg acaccaccaa
    17581 atgcgtaggc atgatttaat ttatctaccg ttccataacc tacgatattc gtataaatat
    17641 ctcggtccaa actgatgatc gttgattttt tttgtttcgg attaactgtt actaccatca
    17701 tcgtatcaga acgtccttgt tccgttcttc ctaatccacc ggtatctaaa cctaatagca
    17761 aaaccgaaaa aggttctttg tcatcaatgc ttactctctc agaacgtttg gaggatattc
    17821 gattagagtt tttagaaatt ttgtttactg tttggctcgc ttctcccata aatctgattc
    17881 cgtatgctga taatccggca atcagcaaaa cgaatacccc taacaaggtt aatagaactt
    17941 tttgccatcg cttcataata cacctctgtt tcacattttc taaaatattg tctcatataa
    18001 ctgttcaatc agcaaattca ctttttaatc ataagaaaac tttaaaaaac atttttgtgt
    18061 aacgattatt attttttaaa atttctcaaa attcttaact atcataccat accgtgaaaa
    18121 agatcgaaag accgaataca cgcagttcgc aatcgtttta tcttttttta tgtatttttt
    18181 ccagaaaaag cgaacaacaa ggtattttcc aaataatcgt gcagtaaaat gatcgcacat
    18241 acctttccca tctttttaac agtctttgcc taccgttcag attatcattc gatttcacga
    18301 caatttcttc ctatgactgg tccttttgtt aaacccatct tctattcgtt ttatttccag
    18361 ctcactgcat ggctcttaaa atgactcgaa ctagtgattg cattaccatc atcttttaat
    18421 ggatagatat gctcctcttc aaagtaaaac gattttcttt taaagtaata gaattgacat
    18481 ggtaatgagt atgtatacta gtggtttcac aaatggtctt ttattcaaca tccaaaaact
    18541 gaaatttctt taaaatccat tactaacgaa gaaaatgttc cttgcttgat aattagttag
    18601 gacatgtcaa gtgaacaaat gcatttactt agccaaaaaa tctaaaaact tcataaatag
    18661 acaaaaagac acgactctct aagaagtcgt gtctttattc aacaaattat tataatttag
    18721 tcacttcagc agcttgaggt ccgcgtgcac cttcaacaat cgtgaattct acttcttgac
    18781 cttcttctag gctcttgaag ccgtctcctt gaattgctga gaaatgaaca aatacgtcat
    18841 ctccgccgtc tactgtaata aacccaaatc ctttttcatt gttgaaccat ttaaccgttc
    18901 cgtgttccat aaatgtatat cctccaaaaa agttcttgca agtaaccttg caagaacatt
    18961 atacccaatt tagaaaacgc tagcaagctt tcgctctttt attttccaaa ttatttttaa
    19021 gactgatatg acggaggaaa tggttatttt gctgcttcga aacgtttgtt tacttcatcc
    19081 caattaataa cattccagaa tgctgcaata taatctggtc ggacattttt atatttcaaa
    19141 taataagcat gttcccaaac gtccaaacct agaattggtg tctttccttc catcaatgga
    19201 gaatcttgat ttgcagtaga ggtaatagct aattttccat tttccattac aagccaagcc
    19261 catccagaac caaatcgtcc agcggctgct tttttgaatt cttctttaaa agaagaaaaa
    19321 tcaccaaaag cttcattaat cgcttctttt atttctcctg taggttcgcc accagcattt
    19381 ggtgccatga tttcccagaa aaatgaatgg ttagcatgtc cgccaccatt gttacgtaca
    19441 gctgtcttga tatctgttgg aatagcgtcc atatcagata ataattcttc tattgttttt
    19501 tcgcctaatt ctgggtattt ctcaattgct gaatttaaat tcgtcacata agtattgtga
    19561 tgcttatcat gatgcagatg catcgtttct tcgtcaatat aaggttccaa tgcgtcatat
    19621 gcataaggta gatctggtaa tgtgtaagtc ataaaaaaat tcctcctcaa tgttttctta
    19681 ataatgtcta cattacaaag agtaacaaag agaaggaaat tcttcaattg ttatgcctag
    19741 tcgtcttttt cactttgacc tacttcttct tcaaaaacct cagtttcttt aaaaataaac
    19801 aacttgctga aaatatagtt cgcaaggata acaataattt gagtgatgat tttcgccaca
    19861 tactcattgc tgttcataac attgatcaac aataccattg cccccatgtc caatccgtaa
    19921 gaaaggatac ggtagaagaa gaacttaccg cattcaataa gtgcatgtgt aaaattcggt
    19981 gattttgaat ggaataccca aagtttgttc gttacaaaag caaacaatac agaacaaaac
    20041 cacgaaatag aattggataa ggccatactt aaatcaaaat attggcgtgc gagagcaaaa
    20101 ctaacaaaat ttacaatcgt agccaaacca ccaaaaaaga gatagactag tatctcccaa
    20161 tatccttttg cttctaaata tccttttaac tgtcgatact gcttcatgag ccaacctacc
    20221 ttattaaaaa ttcttattta tcatactcta tatttcaata actttcatct ttatttaaaa
    20281 aaattaattt tttttgatcc tttgcctttg tctctgcttg gatcgtcaca tgtgtaatac
    20341 catattttaa ttttaataaa gaaattatac gaggatatat cttttcaatc tcgcttagta
    20401 gctgatcttc aaaatcaata tgtccgtcga aaataattat cttttcgcta tactgccaaa
    20461 tatggacatg atgaacatcc tttaccccat cgattgcttc aatttcttgt tccatttttt
    20521 catagtcgat agcaggtgca ctttgtatca aaatagcggc agcttttttg atcaccatta
    20581 tcgcttctat caatatatat atcgaaatga tcagtgtaat aactggatcg atccagtaga
    20641 tatcgaaaaa acggatcaaa attgctccga taatcacact taccgaagac agtgtatcac
    20701 ttaacaaatg caaataacta gattttatat tcaaactttc atctgcttct tgtcgtaata
    20761 ataatactga gaacaaattc gcgagcaatc caataactgc tactgttagc atcaaatgac
    20821 tatttatctt ttctggtgaa gtgaaccgct tgaatgcttc aacggttaaa aacaaggata
    20881 atatgatcaa tatgaatgag ttgatgaaag cactcaatat ttctaaacgc tggtagccat
    20941 acgtacgttt tttatttgct tcttttcggg ccattttttg tgccccgtaa gaaaaaataa
    21001 ttgccaatgt atcactaagg ttatggaaag catctgaaac aagagacaaa ctccctgaca
    21061 taatccctcc aataaattct gataaagtga tcaccgcatt caaaaccact actccaaata
    21121 gtttttttga tgatctctgt ggctgtgtag gcactattcc ttcctccatt ttatttatat
    21181 tggatagtct ttagacaaga ataacatttt tttgatattt caccaatcat tcagctgttt
    21241 ccttaaagca cttgtatttc acttgatact tcgctagaat tgcaagtgat gacactacta
    21301 gatgaggagt acttatgaca acaaaacaat taattatcag ttcctttata cgattcaaag
    21361 acctaaagga ggcgagaaat gtcccatttt ggaaagccat tgtctatctt ttagttttga
    21421 gtataattat ggctttgcca atctcttttc aagtttttca agtcttaaac aacattcaat
    21481 tggatgggca aaagattgct actcgcattc ctgattttac aatccaaaac gggcagcttc
    21541 aaaccgaaga aaaagaagga ttcatttacc aaacaaactc cattatcttc acatttgatc
    21601 cagagggaaa acgaacagaa caggacatct ctactgattt gatggggaat tttgttagtg
    21661 tcgggatgtt aaaaaacaaa cttatcatag ctcttcctaa tacaggcacg acttctgctt
    21721 tgttgaacaa taaccaatta gaacttccct acaccaatga atctttaaaa aacttaacag
    21781 gtaagcagct ccgttctttt ttaagtgaag catctatacc actttggata aaagtcctga
    21841 catttatatt ttctatttat cctagttttc tgaatctgct gatcactttg ctatttacca
    21901 acattgctgc ttttctctat gcacgctttc gactaacaaa agctactttt cttgattgtt
    21961 taaaaacatt gatctattca atttcattgc ctactataat tgcaacaatc ttgatggtct
    22021 ttcttccttc ttttgacact agcgccttca ttgcttttgc aggaatcttt atttttgctc
    22081 aagctgtcaa aggatgggca aaaatatcga tcaattaaga aataaagaca actcgttatt
    22141 tctacttcaa tagacccttt tagaggttgt gacatttttt tgtcacagcc ccttttttat
    22201 ttgaaaaata tttctttctc aaaaaacaag caaggcgctt caaagttctt cttgtatgcg
    22261 ctacacttag agtaaagaaa ggcgggaaat taactatgtc agaaagaatc atttatgcag
    22321 atcgtccatt tcgtgatgaa tttatcgaag aaatccatac tatagcgcct ggttatatct
    22381 ttaaaacaga attacaacca gaggatctac aaaatgtaga gatcagtttg ggctggaact
    22441 cagactacca agaaaaacta ttagcctctt ctaccttgaa atgggtccaa tctatctcag
    22501 ctggtgttga tcagctaccc cttgagactt tttcaaagaa acatatttta ctttcaaatg
    22561 gtagtggcat ccacagtgaa tcgattacgg agcatgtctt aggaatcatc cttgggtatt
    22621 ctcgtggtct ttttcaagca caaaaggcac agagtgacat gaaatggtta ggttcatcgg
    22681 tccattacca atctatccaa gggaaaaaaa tactaatcat tggaactggc catattggaa
    22741 aaatgattgc aaaaaaatgc aaagcattag acatgaactg ctatggaatc aatacatccg
    22801 gtcatccagc agaaggaatg gaaaagactt tttctttaaa tcaactaaag gaagttcttc
    22861 ctgaagtaga tttcgtagtc aatatcttac ctctaacgga ggaaacaact gggctctttg
    22921 atcagcatat gtttgaacac tttgatccaa aatctgtatt catcaatgtt ggccgtggtg
    22981 cttctgttaa gacacaagat ctggtgcagg ctttaaataa ccagcaactt tcctttgcag
    23041 cattagacgt ttttgaggaa gagcctcttc cacaagatca tcctctttgg aaaatggaca
    23101 atgtactgat tacttcccac attgcaggtt tgacacctga ttttcaaaag aaattaatgg
    23161 ctattttttt aaccaatctg aaaagctact ttgccaccca tgagcttcaa accaatcaag
    23221 taaaattgtc tgcaggatat taatagctga aaacaatatt ttcaaaagaa aaaagagaag
    23281 gtgacaaaaa ttgtgtcacc ctctcttttt ttattgtttt ttaaagtgat aggttggctc
    23341 ctccaggttg ggatcataat caacaagtaa atcatatcct ttaaagaacc attcatctgc
    23401 ttcctcgata aaaaaaagtg tcccatcaat ttccactttc tttacaggat gttctggctg
    23461 ttcaactgac attccaatcg aaaaaccatc atgaacctct gttttgccgt agacttttcc
    23521 aaaaaaacgt attccgtaag tttctggcag atcaatttct gagtcaaacc acttttgtgc
    23581 ttttggcgtt accgttaatt tcatcccaac aacctacttt ctcttttctt tctattgatt
    23641 catcttatcg tctagaatat ccacatcgcc tgtctctcca ataaccagta gattatcatt
    23701 ttcacgaaca tattcatctg ctgcaggaga agcgataacc gtttgatcta ctcttcgtat
    23761 ggcgactact gttaagtcaa aacgttgcct gaaatttaat tcagctaatg ttttgttgaa
    23821 aaatttagga ttggagactc ggatttctgc taaagaatat ttatctgaca actctaggta
    23881 atctaaaata ttctttgaca ctaaattatg cgcaatcctt attcccatgt ctcgctcggg
    23941 atggacgacg cgatcagctc cgatcttttc aagtactctg gcatgatact cattttgggc
    24001 ttttgctaaa atatttggta cgcccatttc ctttaccatc agagtaacta agatactggc
    24061 ttgaatatct tctccaatag cgacaattac atgatcgaaa tttcttatgc ctaatgatcg
    24121 aagtgtcatt tcatcctgtg catttgccac aacagcatga gtggctatat tcatatattc
    24181 attgacccga tcttcattac tatcaatagc cagtacttct tgaccagctt cgatcagtgt
    24241 tcggcagata cttccgccaa atcgtccaag acctacaatt gcaaaatttt gtttcaccgt
    24301 tttcctcctt tagtatcttc ttttctcagt ataacaaagc ctttacaaaa acaaaagcaa
    24361 gcatcattat tgtaaaaaag ccaagaaaaa taatagttat tttctcagct tttgattatc
    24421 ctctttatca tcgtttttat ttcaccaaac catgtttgaa tgcatagata gcggcttgtg
    24481 tacggtcttc cacttctagc ttagccaata tattggaaac atgtgttttt actgttttca
    24541 gtgtaataaa caattcgtcc gctatttcct gattactttt tccttcagaa atcagcatta
    24601 aaatttcatt ttcccgattc gttagttctt catgtaatac aggctcattt cgccgactca
    24661 ttttttccat catttttgtt gtcacttctg gttctaatac tcgctcgcca cgttgcgttg
    24721 cccttatagc atcagcaatt tcatgtgcgg tagaggtctt caacaaatac cctgctgcac
    24781 ctgcttcaat ggcaggataa actttttcgt catcaataaa actggttacg ataatgattt
    24841 tagcttctgg ccaatctttt aagattgctt ttgttgattc gataccatcc atttcttcca
    24901 tgactaagtc cattaaaatg acgtccggtc gaagtgtcat cgccttctca tagccttgtc
    24961 gtccattttc agcttctcca attacttcaa tgtcttcttg aatagataaa taaaaagaga
    25021 cgcctaagcg caccatttca tggtcatcta ctaataaaac ttttatcatc cttatctatg
    25081 cctccttcat caaagggacc ttgatttcta cacttgtacc ttgaccttta aaactaatga
    25141 tttttactgt tccaccaata ccagctactc gttctttgat attgttcaat ccataacttc
    25201 ccgttttcgt ttcattcata ttaaaccctg ttccgtcgtc aataatacgt aacaaaagat
    25261 tattgtctat tttgtgcaaa tatacctcta attcattggc tttagcatgt cttaatgtat
    25321 ttgataacaa ttcttgaacg attcggaaca agtgatcctc aatggaacta gatagtttta
    25381 catcttctac atcccatttc agcgaaatct ggattttatt ttgcaattcc ttcaataact
    25441 gctcgatacc ttgctttaaa cttttctctt ctaaattgac tggacgtaga tgaagaagca
    25501 gcgcacgcat ctcagactgg gaagcgttga tgatctctgc taccattttc aactgcttgc
    25561 gaaacatttc tggtgtctcg cttttttctg cttgctccgt caatgccgac atcatcatca
    25621 ttgctgcaaa caattgttgc gaaactgaat catgcagctc acgggctaat cgatgtcttt
    25681 ctaattctaa aatttcttct ttggtttgtc catctacata ttggggacgg ctcgttacaa
    25741 tttgtaattc actagatact tcaatcattt tttcttttat cttagtgatt tccttatcaa
    25801 tatctttgat gtataaatcg tccgatgcac ttgggatcgg aagaagcaac aatttggatt
    25861 cataattgcc agaagaaagt gcacgcagct tttcttcgat tttcccgtat tttgtctttt
    25921 gaataagtga taataataga aagacaatta atcctacacc taaggaaatg gctaacaaat
    25981 ggaagataag cggaacatat aataaacgtg cacgcagtaa ttctccccac caatgacttt
    26041 gattgctggc ataaaaataa gtgaaaagtg aaaatagaat aataaggaaa gccgcaatcc
    26101 ccgaataaac agctagcatc gctctggata tttttcccat catattcgta tcacctcgac
    26161 atctccaatc aaggtattag taataatctt caatctacga gggttttcat cataatcact
    26221 gctgtagatt ttgatttgtt catttttcaa agaaaattgc tcttcttcaa acaagacatt
    26281 tcctactaat gtcgcatgct ctaatcggat ggctacgccc aaaggtacta agatccgtgt
    26341 tctgcctatc ccttttctta caatcacaat attatcatct tttggcaaaa gcgtattgcc
    26401 caaatctatg atagtatcac cagaaatgac ggcgatgttg atatcgtccc attcatagac
    26461 gtcgtttcca atacgttggt taccgaataa ttgctgtctt ttccgctcat tattatgtgt
    26521 ttttaattgc tctgtttgga tcatcatgat ctgtttcttt cgccaaaaag catttttggt
    26581 taaatctatc cctgaaatct ctacaccttt cagaccaata aataaaacgg caaatacagc
    26641 catcatccat aaagcaggac tgttgactaa gctaaaaaaa atgaccaaac ttcctaatat
    26701 caattgaaaa ttttgaaatt tagtacgagg atgtttgcgc atagctagat atatattgaa
    26761 aataccgaat atcactaaaa gcaaaagtcc agtattgttt acgatttgcc aaacagcaaa
    26821 taacagcagt agtgcttcaa ctaccacaaa aaaacgccaa gaactattca tcgctctttc
    26881 tccctcctga cacattctca tttcatcata cctatttttt taactgttgg ctataggact
    26941 ttagatgtaa gatcgtttgt tgaaatctaa aatacacaaa gagaaaaaga tctagagtgt
    27001 atcatcagta agatgaactc tctagatctt tcagtcagac tacgcttgtt ctactttgac
    27061 gattttaact aacatatcgc cacctggagt agcaatagtt acttcatcat ttttacgttt
    27121 gccaatcaat gcttgcgcaa taggagaatc attggaaatt ttgccagaaa acggatctgc
    27181 ttcagcgctt ccgacaatcg tgtactcttc ttcttctcca tctggtagtt cgatgaatgt
    27241 aactgtttta ccaattgata cctcatctgc atctacaccg tcgttatcga tgatttgagc
    27301 aaaacgaatc atattttcaa ttgttgtgat tcttccttca acaaatgctt gttcatcttt
    27361 cgctgattca tattcagagt tttcggaaag atctccgaaa ccgcgggcaa ttttgattcg
    27421 ttcaatgatt tctccgcgtt tgactgtttt tagttcttct aattcttttt ctaatttttc
    27481 tttaccttct agagtcattg gataaacttt ctctaccatg tgtaacgtct ccttttttcg
    27541 ataaaatacc gcaaaaggta atcttttgag gaatatagtc caaaaaagat taccacgctt
    27601 ttcttcaaat gtaaatgttt ttctaaaaat gatcaatgtc tagagcctgt ttttctaaga
    27661 attatttaca tatttttcca ctaattccat atgttcttca taagttttag cgaaataaac
    27721 atttccagtg ctcgtatctg ctacgaaata ataatagtca ttttctgctg gttctaatac
    27781 tgccttgatt gcctcttcgc ttggattgtc aaaaggtcca ggtccatagc cggtattggt
    27841 atacaaatta tatggtgaat ctacagctgt atcttcatac gtcactaatt ctttgtgttc
    27901 acctaatgcg tataaaacag agatatctga ctgaagtggc atatttgctt tgattcggtt
    27961 aaagaagact tgtgcaatgt ttttacgatc attttctttt acgccttctt tttcaactag
    28021 agaagctaga gttaaaactt cttgtacagt caaatttttc tgcttgatag ctgaataata
    28081 gttttgcatg accgtattcg tcttgttcac catttcaatc actagatcct taagcgaagt
    28141 actcgtgtaa taatcatatg tggctgggaa taagtaacct tccaaacggt attttgtgtt
    28201 ctcagcttta gatgcacttt ccaacagttc tgggaatttt tggtgcaact cgttaaagaa
    28261 agtctcatca ttcactaaat ctaaaaattc tttcttgtct ttaccagtta ccttcgctac
    28321 ccgttcagca atctgatcga tatcgtaccc ttctggaatg gcaattttcc catctgcaac
    28381 ttttgttggc tcagaagtac cgccttcttg caactgtttg ccaatctcgt caagagtcat
    28441 attaggcgct aactgataat agccagcttg aaagccagtt aaatttttaa attttgtata
    28501 ataattgaaa acaatcccgc ttttaataat attgtccttc tccaaaatct caccaatctg
    28561 cttgttagaa gagccactcg ggatctcgac ttggactaat tggtcatctg atttatctaa
    28621 aggcttcaac ccgctatcta cataacgata cacggtaaat ccaagaaatc cgccaataat
    28681 caataatgta agagcaatca ctaaaatgat cttcctcaca atcttatctt cttttttacg
    28741 cagatttctc cgcttgtcga ttatatctgc tgtagctgcc ttactggctt cttttttatc
    28801 tttacttggg tgttgtttgt ttcctgacaa aacaactcct ccttactatc aagttcgtaa
    28861 ctttattcat tatacatgat atttgaattg gcgtgaatga ttttatttaa atattctatc
    28921 aaaaatgtaa gtagtaaatc cattactata agattaaaat aaatgttatc aagatacaag
    28981 ctattttagc tttattcttt ctgtaaaaaa gaaaggagac taaataaact attatgaaaa
    29041 aaggaaaaca tgctaaaaaa agaaaatatt catttttaat gatcgttgtc gtgttgttta
    29101 gccttagtgc gttctctaca ggaactattc ttggatggca attttacaat aatgaacact
    29161 ctcagatagt tgcccatgct caatcagctc aaaaaaagtt gatactgact accaatgaac
    29221 aacagctcgt tgaaaaaatc gatcaactat taagaaaaaa tgattatatt ggctcaattt
    29281 acgtaaagaa aaataatcgc atcatcatgg aaaaaggcta tggctatgcg aataaactca
    29341 cgcaacaacc aaatgatcct tctctttatt atcagattgg ttcggttcaa aaagcattga
    29401 ctactctttt gattttaaaa caagtcgagc gtgggcacct ttctttagat actaagctgg
    29461 ctcaattcta tccacaaatt gctggcagcc agcaaattac tatccgtgac ttgctgtata
    29521 tgcgctcagg cttgcaccga actgcttctc caaccatacc gatgactgat gaagaagtcg
    29581 ttcaatttgc gattcagcac ttaaaattta cagattatca gacctctcat tatgattcat
    29641 tgaactttac catattgact ggaattttga tcaaattaac aaatcaaccg tatgaaaaat
    29701 tattgaccga tgatatcatc gaacctttag gattacagca tacaaaattt tatgaccaag
    29761 tcaaaaattc atccaatcat gcactatcct atcaaatgtc taaagaggat gattacttta
    29821 aaccactgac agaatctgaa acagagatcc ataacgaatt agggactggt aatatcagta
    29881 tgtccgtcta tgatttaaat acgctgttta cacaagtttt atctggaaaa ctagtttcca
    29941 aagacttact cttctcatta tggcgtgaaa atgcgaatgg acatccatat agtggtgggg
    30001 tatatagcgg agataattat atcctgtcgc aaggaaatat cgactctttt gatacatctg
    30061 ctatatttag caaagattca caggatgcta tcatcatgga aagcaatata aaagctgaca
    30121 aaactatcaa actgcctact gctgatctaa gaaaccaaat ctatgacctg atggaaggaa
    30181 ctactaagtt ggcaaatagc tgatatgatg aaaagatagc aaatactact tctttgaata
    30241 gcatttgctt aaaaaaagta ctcgactaaa cgtaggaaag agtgtgggac aaatctaaaa
    30301 atcaattttt gctacagact ttaattccaa ataaacagaa cagaaagagg tgttcttctt
    30361 tctgttctgt ccaaattatt ctaagatttc tctttaccaa cattatttga caaagggcct
    30421 attaaatcta aaatagtatc tttagattgc taaaatatgt gctagcaaat cattcatggc
    30481 ttgttcttat ttttaatttt atgcaataaa aaagaagaac aattagcgtg attatagaaa
    30541 taaataatga gattttagaa aaaaatgtgt atgaatacct gattgttact ttatttattc
    30601 cactttcact ttttactgta acaaaaggac ctccttgttt tagtactgta gcattttttg
    30661 aattaacagt aaccgttaat cctttatacc ggaatacagg taaaactatt gttttgcctt
    30721 ttgaagattt aaacgtaaat gtaatttcat ctttatttgg agaaaagcta ctataaataa
    30781 cttgaccatc ttctgtaact tgatggtttt tcaaaagcaa ttgatctttt ttatagccta
    30841 taggtacata atcaaaattc tcaaattctt caagtttatt ttccatttgt tcattagaat
    30901 aagttccttg aaaattcatt tctttcacca tgttaactac tgatgaataa tttaaaattg
    30961 aaatagctaa tattaataat agtataaaat ttcttctcct tgtataagaa aatttttcca
    31021 actgtggaga aataaataga gatactgaaa aagatatgaa caatgttgaa aatatcataa
    31081 aacgccaagg agcttgaata atatttaaag gagtattttg taaggcaaac catggaaata
    31141 tttttgtaca aagtagaata gatattatac ccattatata agtgatttta gataagattc
    31201 ctaatttttt aaagttaaat gttagtataa ttaaacaaat aagcaaagtt aagccaatac
    31261 caaatgatag taattctgaa tctatacttc caatcaaaaa ctctttaccc agataaattg
    31321 ctcttttttg taatatatcc acataaggtc tattcagatc ttgatacaat gtctgctcaa
    31381 tcataggacc tataaacgct agtgaaagaa gtaaagtaac tatagcagct tttattaatt
    31441 taataattaa gaagatttct attttctttt tccgataaaa ttgaataatt aaagcaatta
    31501 taatcattac catagtcata gctacagaaa gaacatgagt gtaaattaat aaagtcatgc
    31561 taatcgccaa cataacccaa gattcttttt ttgagtcgta aagctttata aacccgagta
    31621 aaataagagg aagtattgac attgcgagta attcccccat tgcccctcga agaaatattt
    31681 ctacactcct aaaagaagat gttgtatata taatagcgaa aaaggagcta gtaacatgtt
    31741 ttttggtaat ttcatacatt acataatgac atataaatag agtaatgagc gtaacaatat
    31801 aaagaaaaag gttatagcct attgctaaat tagtaaactt gatcaataaa aataaaggat
    31861 atagagttaa ccatgggtaa aaattattta cttgcatgcc atatgatcta aacgtttcaa
    31921 aattaatagg ggagctaaaa atagattcta aacttaacgc acgattaatg tgaaatgccg
    31981 tatcataaaa gccaaatatt tccatgtttt taaatgctgg aaataaatat aataaactaa
    32041 caaatgcata aactataaat atttttacag aaaaaatttt tttcaaattc atataccacc
    32101 tcatgttttt ttatattaca tatagtatca tttatttttt ttaaacacta gattagtcac
    32161 tatataaaat gtgattaatt ttattattaa taaaatattt tttatattac aaaagtagtg
    32221 ttt
//
LOCUS       DAYLZO010000017        30048 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.017, whole genome shotgun sequence.
ACCESSION   DAYLZO010000017 DAYLZO010000000
VERSION     DAYLZO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.017"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..794)
                     /locus_tag="ACGVJG_000752"
     CDS             complement(<1..794)
                     /locus_tag="ACGVJG_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFJ6556730.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFIT"
     gene            complement(904..1473)
                     /locus_tag="ACGVJG_000753"
     CDS             complement(904..1473)
                     /locus_tag="ACGVJG_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFJ6556731.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(1491..2906)
                     /locus_tag="ACGVJG_000754"
     CDS             complement(1491..2906)
                     /locus_tag="ACGVJG_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556732.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(2917..3585)
                     /locus_tag="ACGVJG_000755"
     CDS             complement(2917..3585)
                     /locus_tag="ACGVJG_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFJ6556733.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(3715..4299)
                     /locus_tag="ACGVJG_000756"
     CDS             complement(3715..4299)
                     /locus_tag="ACGVJG_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6556734.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(4630..4863)
                     /locus_tag="ACGVJG_000757"
     CDS             complement(4630..4863)
                     /locus_tag="ACGVJG_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFJ6556735.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(4878..5828)
                     /locus_tag="ACGVJG_000758"
     CDS             complement(4878..5828)
                     /locus_tag="ACGVJG_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFJ6556736.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(5825..6667)
                     /locus_tag="ACGVJG_000759"
     CDS             complement(5825..6667)
                     /locus_tag="ACGVJG_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFJ6556737.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(6669..7388)
                     /locus_tag="ACGVJG_000760"
     CDS             complement(6669..7388)
                     /locus_tag="ACGVJG_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556738.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            7936..8238
                     /locus_tag="ACGVJG_000761"
     CDS             7936..8238
                     /locus_tag="ACGVJG_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556739.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            8310..9536
                     /locus_tag="ACGVJG_000762"
     CDS             8310..9536
                     /locus_tag="ACGVJG_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6556740.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(9624..10016)
                     /gene="rpsI"
                     /locus_tag="ACGVJG_000763"
     CDS             complement(9624..10016)
                     /gene="rpsI"
                     /locus_tag="ACGVJG_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFJ6556741.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10032..10460)
                     /gene="rplM"
                     /locus_tag="ACGVJG_000764"
     CDS             complement(10032..10460)
                     /gene="rplM"
                     /locus_tag="ACGVJG_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFJ6556742.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(10994..11461)
                     /locus_tag="ACGVJG_000765"
     CDS             complement(10994..11461)
                     /locus_tag="ACGVJG_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFJ6556743.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(11581..13119)
                     /locus_tag="ACGVJG_000766"
     CDS             complement(11581..13119)
                     /locus_tag="ACGVJG_000766"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFJ6556744.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAKKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            13199..13885
                     /locus_tag="ACGVJG_000767"
     CDS             13199..13885
                     /locus_tag="ACGVJG_000767"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFJ6556745.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(14086..14613)
                     /gene="msrA"
                     /locus_tag="ACGVJG_000768"
     CDS             complement(14086..14613)
                     /gene="msrA"
                     /locus_tag="ACGVJG_000768"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFJ6556746.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14697..15374)
                     /gene="pgmB"
                     /locus_tag="ACGVJG_000769"
     CDS             complement(14697..15374)
                     /gene="pgmB"
                     /locus_tag="ACGVJG_000769"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFJ6556747.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(15371..16501)
                     /locus_tag="ACGVJG_000770"
     CDS             complement(15371..16501)
                     /locus_tag="ACGVJG_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFJ6556748.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(16501..18684)
                     /locus_tag="ACGVJG_000771"
     CDS             complement(16501..18684)
                     /locus_tag="ACGVJG_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFJ6556749.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(18681..19706)
                     /locus_tag="ACGVJG_000772"
     CDS             complement(18681..19706)
                     /locus_tag="ACGVJG_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFJ6556750.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19751..20578)
                     /locus_tag="ACGVJG_000773"
     CDS             complement(19751..20578)
                     /locus_tag="ACGVJG_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFJ6556751.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(20575..21642)
                     /locus_tag="ACGVJG_000774"
     CDS             complement(20575..21642)
                     /locus_tag="ACGVJG_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFJ6556752.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(21668..22504)
                     /locus_tag="ACGVJG_000775"
     CDS             complement(21668..22504)
                     /locus_tag="ACGVJG_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6556753.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(22501..23370)
                     /locus_tag="ACGVJG_000776"
     CDS             complement(22501..23370)
                     /locus_tag="ACGVJG_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6556754.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(23440..24723)
                     /locus_tag="ACGVJG_000777"
     CDS             complement(23440..24723)
                     /locus_tag="ACGVJG_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6556755.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24737..26422)
                     /locus_tag="ACGVJG_000778"
     CDS             complement(24737..26422)
                     /locus_tag="ACGVJG_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFJ6556756.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            26697..27722
                     /locus_tag="ACGVJG_000779"
     CDS             26697..27722
                     /locus_tag="ACGVJG_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6556757.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(28317..>30048)
                     /locus_tag="ACGVJG_000780"
     CDS             complement(28317..>30048)
                     /locus_tag="ACGVJG_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFJ6556758.1"
                     /translation="RMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVETITK
                     QFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISNFTQ
                     LAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSGYLT
                     RRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPETNE
                     LIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVEVGE
                     AVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVITEVT
                     GDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSIDPK
                     QLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTDILP
                     GTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRVLTD
                     AAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEAQMA
                     AEDAMNNQTK"
BASE COUNT         9034 a         6081 c         5025 g         9908 t
ORIGIN      
        1 gttataaaaa aggttatagg aactatcaaa atgcaaacta tgccaaaaaa tagccattga
       61 aaaagaacaa aaggtgtttt cccactcaaa gaatacttat atgcaaactt agcataatcg
      121 ttaattgctt tattcaaggc tttaaatgat gttactttag caccaaatat ctttataact
      181 tgaattccgc ggatatattc taccgtttca gagcttaaca catctaacga ttcttgatat
      241 ttttgaataa aatttgtatt ctctgtcatt ttctttaaca agaatacacc aattactaac
      301 atggcgatta gaaccaatcc aattttaaag ttaatgaaaa agcctaaggc aactgataaa
      361 acaggtgtta ctatggcttg agcactatct ggaatcaagt gagcaattgc catatgtgtt
      421 ttagccgcgt tgtcatcaat tgtctttcga atatatcccg aagaatttaa atcaaaaaaa
      481 cgaaaacttg cttctgttaa accgctaatt cccttttttc tcaaattagt ctctaagcga
      541 aacccaagga tatgagaaaa taagccagaa aaaatataaa ataaagctcc taaagttaac
      601 caagcaacag tttgaaccgc ataactctta gctaaatatt catctcctac aataattaga
      661 gactgaagaa atttgtaaat actgtaatat ccatagactg ttaatacgac agaaaaatca
      721 gaaaataaag tggctaacca tccgtagtat ttcattttag ggacataact aaataatttt
      781 ttgtatactt tcattttttc ctcccccgca ttcaatttct tctcttattc gtttacaact
      841 gttaatgctt tccgtaatag cgtaaattag agatgtacaa acagataaaa ggtaaactac
      901 gttctatttt ttgtattttt ttaagacttt tcttccaatc aatacaccaa tcatagataa
      961 acttgctaca actaacactg aaatgattcc ccatatacct accgcatagt ggatcattgc
     1021 atccattgtt gcagcagatt gtcctttatc tatccaatct tgacgaaaac gatctgtaaa
     1081 gaaccatata ggaatccact gtccggcagc actgcctaat tgcattagag cccaagacgc
     1141 aacttgagga attgctcgat catatccaaa tacagataga ataatatcag ctaagatcgc
     1201 catactaatc gaactgataa accaaggtaa gtacgccccg cccattaacg taaaaaataa
     1261 caataaaaca attgtgtaaa tagtaaaaat tccttttcta gggtagttat agctaatata
     1321 aacaaatatg attccagata ataatccaaa aatcccgggg gatatgctgt gaccaaacgc
     1381 cccaccaata ctactaacca ttattgaaaa aaagtaaatt actagggaaa taactgtagc
     1441 taaaccaata aaaatataat cttttgtttt catatatatt ctcctcaatt ctatttcgtt
     1501 atgtcattaa aaatctgttc tatcaaagtt gaatcttcta ctctaagttt tatatctttt
     1561 tttattccac cattttttaa ataaataatt ctattgcatg ttttataaag aaattccgga
     1621 tcatgagtag ctataactac tgcacccttt tcttttaagt ctttaataag gtcagcaatc
     1681 ctttccatat taatataatc caacccactt gtaggttcat caaatagaaa taaattagtt
     1741 tttgacaatg aagctataga aaccaataat ctttgttttt ctccaccgga taaatcaaac
     1801 ggtaattgat ctttcatttt ataaagttcc atttttttta aaattttttc ttgctgatct
     1861 ttactaacta tttcattccc cagggctaac tcgctcctca ctgattctgt gaaaaattgg
     1921 taatccattt cttgcattac taaaaacggc gatgacaaat tttttttatt ttcgattatc
     1981 tttcctttgg tagggcgaat aatcttcatg agtattctta gtaaggttgt tttccccaca
     2041 ccgtttttcc ctaccaaacc aataacatct ccaactttga catcaaaatt tatgtctttt
     2101 agaatatttt cataagtaat ttcttcaact tttaatacat ctgcacttac cttttcgata
     2161 tagggaactt caatcgaact attaaaaatg tcaaatttcc ttaatgcgtt attgttttca
     2221 gtttttaaca actgaatagt tgtagttttt acagttctat ttttaacaaa tagaacttga
     2281 tcaattaatt ctttcaaata gtaaaaacga tgttcagcta taacaatagt aaatccttta
     2341 ttttttaaat cacaaagtaa gtcagcaagt ttcttagtcg ctttgaagtc caaatttgaa
     2401 gacggttcat ccaatataat taatttagag tttagcgata aactacatcc aatagaaatt
     2461 ttctgccgtt caccacttga tagcgaaaaa atgtttttat ctaatatatt tgaaagatct
     2521 aataattcag ctaattcatt gattctttta tccatatgct cttttgtaaa tccaaagttt
     2581 tccatcgaaa atactaactc actcgttgta tttgttgtaa agaattgtcc tctaggatct
     2641 tgacttacgt agccaatatt ttttgatata ctttcttgtg ttaaggattg tagcgagtta
     2701 ttttcaaata aaatttcccc aaccaatttt ccgtcataaa gtcttggaat aagtccattc
     2761 attaacttta atagtgtgga tttcccactc ccagaagaac cagtaatcaa tgtaagactg
     2821 ccttttttta ttgaaaaatt aaggtcaaca agcgcttgtt ccttaacatc cttatatgaa
     2881 aaagaaacat tgttaaattc aaacaattct ttcacctcaa atcaaaatac ttaagaaaaa
     2941 aatagtaacg gaaaacagga tgatatagtc taataagctc atgtttgttt tatagtaaga
     3001 acttcttttc acaggagatt ctatcccctt tgttattgca ttagcggtca tttcttcagc
     3061 aatcatagtt gcacgtataa gaacgggaac taacagaaat tccaaatatt gcagaggctg
     3121 aaaaaaagac aagcgaatgt ttctcatttt taaagattct ttaatcattc ttatttcaga
     3181 acgataagtc ggaaagaatc tgatcactac tgtaatactt aacattattt ttttcggtaa
     3241 atgtattttt tctaaagagg ataataattc attgctagta tagacactaa ccaacaacca
     3301 tgagatcatt acgaccggca acattcgaaa tacaatatat aaggatgtcg caaaaaatat
     3361 agtaaaaaac tctatcttga tagcatcaat aattttcatt actaaaaaca gaactgaata
     3421 aatcaagatg aaataaatac atgtctttat tttacgaaac atcaatacta aaactgtagc
     3481 aataatgaaa caccccactt gaaatatatt tccctttcct aaaaaaataa tgaccggaaa
     3541 aaaaatattt aaagtaatca aggttaacgg attgattttt gacactcttt cacctccaaa
     3601 aaataagatc gcactgttat cttaagctga taaaaatgca ccgttattca gataacagtg
     3661 cgatcttatc aaaaagcata actgagaaca attctcaatg caagcttttt ttaattaact
     3721 cagtaattca atccacccag gaacagaaaa ttgatacaat cttctggtta atagcatagt
     3781 cttttctttt gaatagctat gcttcaatat atcatagaat gtagcgtaga atgagtaaat
     3841 gtagaagtgg atttcttctt cattaaagac taaattttga ttgatcattc cgatagtctt
     3901 taacttatct agacaacaga ctgtcttttt cgtcacaaat gatgtaattt tttcaacaat
     3961 atcttgatat ttggttccag ctgattggaa taaaatcaaa tcgaatatac gtttattctc
     4021 aaaaatataa ttaataaatg gatctaattc attgaagttc tctctccaac ttttctctat
     4081 atctatattt tctaagttta agaggtattt ggtaaactct tcattataca tattaataag
     4141 cgcattaata tgcggctgaa taatttcttc aaataacgct tccttagaag taaaatgttt
     4201 ataaaaagca cccgtagtta cgcctgcggt caggcacagt tgccttatct tagtttgctg
     4261 aaatccttct ttttcaaaat ggcttagtcc actttctatt aacttttggt gagttataat
     4321 ataatctctg gtttctatgg gtacttcctc ctaataagat gaattaactt tattatctta
     4381 cacaggactt gaagtttcaa tcgttaacgt ttattactga actcattcct tagagctaaa
     4441 ctaatgagaa agagcagaat aactgataca aaaagcaatc ctacaagaat taatagactc
     4501 atctaaatca ttcacctttt ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag
     4561 ctattaggat aagtcctatc ataatcactc caatagccat tttcttatcc tccctaaaaa
     4621 taaagtcttt tacttaaaac cttttttgta atcgtcaata aattttttcc ctttatcagt
     4681 taattgaaat cctatatcta aatagtcctc atatctaagt ttagccagca tctcacttac
     4741 agatgctgga ctaacactta gaacttttgc taaatcatta ttttttaatt tcttttttct
     4801 ttttgataat tcctgaattg ctagtaaata aattagtata gtagtcgttt taatttgtac
     4861 catacctacc ctccaaatta tttcgaaagg ccttcagcaa ttttatccag gttccatttc
     4921 atcatcgcat agtaactatc accatcttgt cctttttctg caattgaatc agtaaaaatc
     4981 gttgaataga taggaatatt ggtatctttt gatactgttt tcattggtct atcatccaca
     5041 ctgctttcta cgaataagga gggaactttt gttgtgcgta atttttcaac taagtgtttt
     5101 atttgatctg gtgttccttc ttcttcagtg ttgatttccc aaatgtaagc agaaggcaca
     5161 ttatacgctt tcgagaaata tttaaagcat ccttcacttg taacaatcat ttttttatca
     5221 ttcggaattg aagcaaattt agatttagct tctttgtcta gagaatccaa tttttcaata
     5281 tacttatcta gattttcttt atagaatttt ttattatcag gatctttttc cgctaactgt
     5341 ttttcaatat ttttagcgta aataataccg ttttctaaat ttaaccaagc atgaggatct
     5401 tccttccctt tctcactctg accctctaag taaataacat ctatgccatc acttgctgca
     5461 aaatagtctt tgttttcctc tttgttcgca tttttaacta attttgtaaa ccaagcattt
     5521 cctccagttt ccaagttaac accgttataa aaaatcaaat ctgcttttga agttttttga
     5581 acatcttcag gcaaaggttc atattcgtgg ggatctttcc caataggtac gatactgtgt
     5641 aaatcaattt tatcttttgc tatattttcg gtaatatctg ctaaaatcga attagtaact
     5701 actactttta atttttcgtt cgaagccact ttatctttgt ctttactatt tattgaacat
     5761 gccccgaata taaaaagagc aatcaaaatc cccagacttc ccagtactgc tttccatttt
     5821 ctcatcataa cgatctctcc ttctttctta aaaatttttg ttttggtgag attagaaaac
     5881 taattacaaa taagaatgcc gctgttaaaa caatacttga tcctgctgct atgttaaaac
     5941 tataaccaat aaacaatcca agcaatgatg aacttgctcc gactgttgca gaaataaata
     6001 tcatagtttt caagctattt gcatataaat atgctgtagc tgctggtgta attaacattg
     6061 caacaattaa tatagttccc acactttgca tagctgttac tgctactaaa gttaatagaa
     6121 ccatcaatag ataatgataa aaactgacat tcatccctat tgcttttgcc attaatggat
     6181 caaaagaagt gattaataat tctttaaaaa atagaactac tataaccaac actaaaaccg
     6241 aaacgccaat tgtaatccac ttatcaatat cttgtacagc taaaatattt ccaaataaaa
     6301 tatgaaataa atccgttgag ctatttgcaa ctcctataag gataactcct agtgctaaaa
     6361 aagaactgaa agtaatacca attgctgtat ctccttttat tacactattg ctttttataa
     6421 aagtaataat gatagaagct aatagaccaa atacaatcgc accaataaaa aaattaatac
     6481 ctaaaatata agaaattgct actcctggca aaacagcatg agaaatagca tcacccataa
     6541 gagacattcc tcttaaaata ataaaacaac ctactgttcc agaaacaatc cctataacta
     6601 cagatgtaat tagagcattc tgtaaaaagt gaaagtcata cagaccatta ataaaatttt
     6661 gaagcatatt attccccctc tccaataaat atagtatctc cataggcttt ttttaaattt
     6721 tcttcattaa atacagattc tacacttccg tgagcaatta gatttcgatt caaaagaata
     6781 atattatcaa aatacttctt taccttgctt aaatcatgat gcacaattag cactgttttt
     6841 ccctgatgtt tcaatttttt taacgtatcc ataattattc gttcactaac taagtctatc
     6901 cctacaaatg gttcatctaa aaatatgaaa tccgcatttt gagcaagaca tcttgctaaa
     6961 agtacacgtt gaaattgtcc tccagataat tctcctattt gatgattaga atattttccc
     7021 atatcgacct tttccaaagc ctttgaaact ctttgccatt ctgcattttt aattctttga
     7081 aaaactttca ttccggcata tgttcccata gatacacatt cttttacttt tattggaaaa
     7141 gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct tcaccagttt tttattgata
     7201 tcttcattat taatggagac ctctccctga tgagggataa tatttaacat tgctttaagc
     7261 aatgtggatt ttccagcacc atttggacca ataatacctg ttaaagcacc ttcttgtatt
     7321 tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac aaacattaag ctttttcact
     7381 tctaacataa ctttctcctt ctaaatgata atgattatca cgatcatatg gtatacaaaa
     7441 attatatttt agtcaaccct aaattttata gtttagattt ttttctgttg ctgaagatta
     7501 gaatgacaaa actatttgaa actacgcgta tagtaagtag ctttcaaaat atataaaaaa
     7561 ggagcgttct caaatgagaa catgctccta aaactacttt attggcgtat ggaaaaatta
     7621 tacttgaagt ggatagcggg ttgaataagt tgataagcat caaaacattt ataaaaatta
     7681 ttcaagacta tgtctgttcc atatacaaat atgaaaatca gtaaactcca tttaactttt
     7741 ttcgtagtgt gtctttttat acatgtttat cgttattaat agtgaatagg aaaaaaacta
     7801 tttttgatca ttgacaatta aatacaattt ttgaataacg atccctgcta ttcgagctag
     7861 aaactcaagt acgccatgac ctgattagcg acaaatactt agagatttct ttttgttctg
     7921 ggaaaacaaa atgccatgac aatagcagca aataatgata cttccgtaag gctcgccgta
     7981 aactggggag aggtgaaatt cctatgtgct tacccaaagc aattcaaaaa tttcataact
     8041 actattttag gagatgattt tatagaatcc agagtctacc tgaataaaaa acaaacctgt
     8101 gcatttctag gaatcgataa taatactcta gataagtgga ttcgtatata taatttccca
     8161 gtaataagag ttggtggtgt agttcgcttt gacgtagaag atattaaaga ctttatgaat
     8221 acatttaaaa taacttgaaa ttttcttctc gccgcatata ctaaaattag tgaaatatac
     8281 tgccttactt tttttaagga gtgaggattt tgcctacaaa gctttcaaat gggaaatata
     8341 aaactaatct gagatatcca aaaaggttta aagaaataac aggaattgca tcagaaaagt
     8401 atcaaaaaac atttccaaat aggcaattgg cgattaaagc cgaaaatgat atgaagaaga
     8461 aaatagaaaa agttttgaga gaggagaatg caaactcatt agaattaaaa ggaaaaataa
     8521 cgtttaaaaa attttacgaa tctaaatggc ttcctagata tgaattaggt caaacaatta
     8581 gatcaaacag gcctcctagt gatataacca ttagtaatac aaaagatata tttcggttgc
     8641 atattttacc tatgtttggt gaatacgcaa tgaattatct aaatatcaat actgagataa
     8701 tatcagatga attaaccaaa aaatcgaaag aatatgcaaa tataaaaatc ataaaaggat
     8761 atgttcgttc catgttcgat attgcagaaa tactaaatta tatcgaattt aatcgaacta
     8821 ctaaaattat tcaaagtatc actgcaccta aaaaaaatgc tttagaagaa aaaagaatac
     8881 aagaaggtaa gcaagcacta tcatcaaaag aacttactaa ttggatagaa gcagtaaatg
     8941 atgattttaa taatcattta cttacttttc atgattacac actttttatg attaccctgt
     9001 atcttggtga tagaaaaagt gagacttacg ctttgcaatg gaaatatatt gattttgaaa
     9061 aacaaacggt ccgtcttaaa catactttag ataaatatca aagaaaaaag tttacaaaag
     9121 gtcgaaaaga taccgtcatt caagtaccag aagttgtaat gacattgcta agtgaatgga
     9181 aatcggtaca agctgatcaa ttgttgaaat taaaaattaa gcaaacgtta gatcagtatc
     9241 tctttactta tactaaacca tctggagaag taaattgtcc tgttcatgcc gattacctaa
     9301 actatcgaat caattcaatt aaaagaaggc atcctgactt agttcattta tctccacaca
     9361 aactccgtca tacttatgca actatagcta gacaaggtgg agcagatatg aatcaaattt
     9421 caaatgcttt aacacactca gatatttcta caacaaagat atatgtgaac acaccagata
     9481 tagttgataa ggctgttttt gaagcttttc agagaggatt gaataagtgt gattgatatg
     9541 tgttcttaaa tgtgttctta aaaacaaaag aagcactttc caaataattg aaaagtgcca
     9601 gaaacgttga tatataaagg atattaacgt tttgagaatt gtgaagcttt acgtgctttc
     9661 ttaagacctg gttttttacg ttcgaccata cgtgcgtcac gagtaagtaa gccagcacgt
     9721 ttaagagcta aacggaagtc aggatcaact tctagcaatg cacgagcgat accatgacgg
     9781 atagctcctg attgtccagc gtatcctccg ccgtttacgt taacaaatac gtcatatgcg
     9841 ccttttgttt cagtaacagc gaaaggttga ttgattactt cacgcaagtc agcatgtgga
     9901 atgtattctt caacatcttt tttatttaca gtaattttgc cagttcctgg tactagacgt
     9961 acgcgagcaa ctgcgttttt acgacggcct gtgccgatat attgaacttg tgccaatgaa
    10021 attccctcct attagattaa gtttgtgatg tctaaaactt ctggttgttg tgctgcatgt
    10081 ggatgttctg ctccaccgta tacgttcaat tttgtaaatt gtttgcggcc taaagtgttt
    10141 ttaggaagca taccttttac agaagtttcg atcaaacgac gagaattttt agcacgcaat
    10201 tcgccagcag tgattgattt caatcctcct gggtagttgc tgtgacggta ataaattttg
    10261 tcagtcgctt ttttacctgt taatttcact ttgtctgcat tgatgacgat gacgaaatcg
    10321 ccagtatcca aatgaggtgt gaaagttggt ttatttttac cacgtagtac agatgcaaca
    10381 actgttgaaa gacgtcccaa tggaacatca gttgcgtcta ctacatacca tttacgttct
    10441 acttcgcctg gtttggccat atatgttgta cgcacgttat tttcctccaa tttaagttct
    10501 gatgtttgac atacacttcg agtttccggg gctctttgtg gggcaaacaa taccatttaa
    10561 catattaccc gctaatctag tcaatgtcaa cgtttttctg cttgattgaa aaatttatat
    10621 gaaactttac ttcttttctt tcttttatat aaaagggtcg tttcctgtca aaatgaaaaa
    10681 caaaaagagg ctgtgacaaa agtcttgtca caacccctta ttttgaataa acggtgttct
    10741 cctgccaact cctcggtcac aagtcacaat atccataaaa cataagaagc tgttttttaa
    10801 aattgtttct tgtgactcgg agctgaacgg caggcttaca acctcttggt aaacggtgtt
    10861 caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgac
    10921 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt tcggtcaagt
    10981 tcaacaacag tttttacgcg tcgatctctg gcgctttgcc caaatgagct tggatcatcc
    11041 agatacgttt ttctgtggct gttttgaatt cgataaagat atcttgtgtt ggatcgtcgc
    11101 cttcttctcc agaaacttcg attccttttt ggtaaagatc tgctagataa cgatagcctg
    11161 caacaagtgt ttccaaacgt tctggaatag tacgatccca gcggccaatt tcatcaggga
    11221 tttttgtgtt atctgcaaat tcacgcaatg tagaataagg tgaaccgtct aatgtgatca
    11281 agcgttcgga tacttcatct aactgagcgt tcaaatcatc catgaactca tccatcattg
    11341 ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg tgtttggtga acaacaacag
    11401 acatttggct tagatctgca actaattgat ttaatacttc tttggtttgt tggaatttca
    11461 tctttcttgc ctcctaacat tttcttacac ttaaaataat agtagacttt caatgaaaag
    11521 tacaacaaaa tcatttacct gagaacgatt ttcagttcgc ttttttatcc gtaaagccaa
    11581 tcatttttca gaatcagcta attcttctcg cagttgagca aattgctgat aagtctgtgc
    11641 taaatgcttt tgtgtcgaaa caaataatgg atagattttc tgatattttt tatgatcagt
    11701 ttcttgtact ccaacttgct ctttcaattc tatcaattcc tgtgcttcac tgatactttg
    11761 gatctttccg atgcttctcc agcctaaaat cacagctcct aatgcggatg cttctgtgac
    11821 agtagggaaa acaagcgggt aacccagtac atcagccatc atctgacgaa aaatcggaga
    11881 ttgagcaaac ccgcctgtag cacggatctc tgtgagctga ttttcctggc aaactccttt
    11941 taaaatccga taaaggttga gacagatccc ttctagaatc gccttcatca tttctttttc
    12001 tgtatgttgc cgctgtaaac cgataaaact gccaaacgca tctgcgtccc aaagcggtgc
    12061 tcgctcacca agtaaatagg gatgaaaaat cagaccattg gcacccgctg gcacaaaagc
    12121 gatagccgaa agcagtgcat catatggatt ttctcctttt tctttagcag attgaacgac
    12181 ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca gcaccattgc tagtcgctcc
    12241 tcccactacc caatgtttct gatccagaac ataacaaaac gtctccattt ctggatggaa
    12301 ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt cctacggtca aagcggcgat
    12361 cgtatcacca aaagcaccta accctagatt agaaagcggc ccgtctgccc ctcccaaaac
    12421 aaatgatata tcatttggca aacctagttc tgctgctgtt tttgatggta gacctgttac
    12481 ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt cgaatgttca agtagtcaag
    12541 cacttcttct gcccattgga attcatggat atcaaaatat ccagttcctg aagctgttcc
    12601 gtagtcgctg atcagttcgc caaacaatct ataaaagata tagtctttga taccgacaaa
    12661 ataagcagtt tttttgaata attcaggttg gctgttttgc agccacttga ttttgttcaa
    12721 tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa aattctttgc caagcacggt
    12781 atatttcagt ttttttgctt cgtccgctgc tcggttatct gcccaagtga tcattcgaga
    12841 taacggtgtt ttctcttcat ccaacaatat caggccatgc atcgcagagg agaaggagac
    12901 aaattgaatg attgtgtctg aaggcaactg tttaattact tcttttataa cggtgcagac
    12961 accttgaaag atcaattcct gatcttgttc tgccatccct tgtgtttctt ggatcaaagg
    13021 gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct gtgaaagcaa ctgctttggt
    13081 ttgtgtggtt ccgatatcta atccgattga aacgttcatt ttttcacctc aaattttatg
    13141 tatgtacaaa aatttagttt aagcttgcgt atcttggttt tgaaaagggg gagatggcgt
    13201 gttactttat tttatcattg gttgctgtat cggatcattt ttatgtttaa ccgcccagcg
    13261 gattccgtta ggtcactcta ttatttatcc tagatctcat tgtgtcaaat gctgtcgttc
    13321 gctttcttgg tatgaattga ttccgattct ctcgatcatc gtccaacgat ttcgctgccg
    13381 ctattgcagg tgtcggttgc ccttctacta tttgttggca gaagccttat gtggtggtct
    13441 ttttgcctgg ttctttactt tttcttccgc aagaaatttt tcgacgttca tatggatgct
    13501 ttcggctttg ctattttcgt tgatggattt attttacttc atggtccatt ctcacacgct
    13561 attctgttct tgggcaattt tatggatttt ttggttgcag gccggcgtct tccaatggca
    13621 aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt cgctatggtc aatcctatct
    13681 cggagctggc gatactctgt tgatgctttc ttggagtggt ggcttgtctc tagaagagtt
    13741 gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc gttttttctg tttactttat
    13801 ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct tttttgagca gtgcgttgct
    13861 gattgtcctt catcttaggt attgaaaaaa ggttgtgaca aacgacttgt cacaacctct
    13921 gatatatagt gttctcttac caaatccttg tgacttggag ctaggcgaca ggttcacaac
    13981 ctctccacta aatggtgttc aatcagctga ccctcagtat tagctcaaaa tgctggtttt
    14041 acaacctctg atatacggtg ttccataagt agctttttcg gcttcttatt tcttgacgcc
    14101 ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct cgataaagat tgtaatggag
    14161 cttgtttttc ttgtaataat cctgatggta atcttctgca ggataaaatt cgcttcttgg
    14221 ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg tcttgcaatg cttgttttga
    14281 acgctctgca atatttcgtt cttcttcatc attatagtag atcactggac gataagaatc
    14341 accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg gtttgctgcc aatagatttc
    14401 caccaactga tcataagaaa taacttctgg atcatattcg atctctactg cttctgtatg
    14461 ccctgttgtg cctgttgtta cttgttcata agttggattt ggtacatgcc cgccagtata
    14521 accggatgtc acagaaatga tccctggctg ctcttcaaat ggtttgacca tacaccaaaa
    14581 gcatccgcct gcgaaaactg ctttttttgt catataaaat cccccttttt tctcatcata
    14641 ccttgatttc caaaaggagg ataacgtttt gcttgtgaca gaaaaaacgg aaactattat
    14701 atacgtgata cggcttctga taaatgccaa aacgaacgta cttgatcaaa aacggttaaa
    14761 tcaggaatca catattcagc agaaggaaga tttatgtttt cacctattcc caatacatgc
    14821 atgttaccag ccaaagctgc ttgacaacca gcttctgaat cttcaatgac caagcatgca
    14881 tttggcggta cattcaattg ctgtgctcct ttgagaaaaa cttctggatc aggttttgct
    14941 tttgatactt gcgtgccgtc aataaggaca tcaaaataac ttgtcaaatt cgttttttct
    15001 aaaatgaggc gtgcattttt gcttgctgat cccaatccga tcttgatgtt tgtttttttc
    15061 aagtactcaa gaacagctat tgcaccaggc aaaacggatg tttcatccat tttttgaatc
    15121 gcttgaagat atagattgtt tttttgctca gcaaaggctg atttctcttt ttctgagaat
    15181 ttctgttcca tttttccaaa tgacagcaat cgctctagtg actcggttct ggatattcct
    15241 ttcagttgct cgttttcttt ctcagtgaat ggaatggaca gctggttagc taaaaccagc
    15301 cacgcttcgt aatgataacg agctgtgtct acaagaacgc catctaaatc gaatagtgct
    15361 gctttgattt tcatcgatta cctccttgaa aatgaacgac ttgaagtaaa gatgcgatgt
    15421 ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa agcttcttgt aaaggcacag
    15481 ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc tgctgctttt tcgcttcctt
    15541 taccaacact tccgcaaata gctatcacag gaacgccctc tggtactctt ctagcaatcc
    15601 caactggcgc ttttcctttt agactttgtc catctagacg tccttcgcct acgattacga
    15661 ggtctgcttg ctgtacccgt ttatcgaact ccagtaaatc caatacgtag tcgatgccag
    15721 agactatttt ccctgaagca aatgctaaca gtcctgcacc cattccacca ccagcactac
    15781 tgcccgatgc ctcgattact tgaggggcaa atctcatata aaaatcatgc atggcttggt
    15841 cagccactgc taattcattt tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc
    15901 cttcttttcc gcataaaaga ttagtaacat cagtaatgac agtgattttg gctttaatca
    15961 gctcaatagg cacgttcgtt ttatcaactg tagcaatttt acctagattt tctcccagtg
    16021 cttctacttt ttgattatct tgatcgtaaa aagcataacc tagaccttct gccatgccga
    16081 tgccgccatc gttggtacta cttccgccaa cgccgatgat gatgtgtcga acaccttgtt
    16141 gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga taacctaagt ggctgttgtt
    16201 ggtttagtgg gacatctttc aatccgacga tttctgccat ttcaaaaatc gcagtttgac
    16261 cgtctgaagc gtactggatt tctttgtttt tgccaaatgc attagtaacg actgctgttt
    16321 gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt tccttctcct ccatcgccta
    16381 taggtagcag atcatattca gcctgaacaa aaacttcttg aaaacctgat tgaatggctt
    16441 gtgctgcctc taaggctgtc agactttctt tgaaagaatc tggcgcaatc agaatacgca
    16501 ttattttccc ctccgttcca agatcagttc tttttccagc tggtattggt ctccattgac
    16561 aaaaataggt aatgtttctt tcgtctcttt ttttattact atttgttttt ttgtcaaaac
    16621 aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa attttttccc atgctgaagg
    16681 aagtcttgga gaaatagaaa gagttccatt cgttactgta atacctgaaa aaccaaatat
    16741 aactgctagc catagtgctc ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg
    16801 atctggtcct aaatcaatca ggcacgcttc ttggaataaa tgatacgccc attccatttc
    16861 ttggatacgg caagcctcta tggcgtgaat cgctttactc aacgatgagt cgtggattgt
    16921 tttgttttca tagtaatgca aattcttaga aacgattggt tgagaaaaat actcaggtaa
    16981 taagtataac agcatgacaa gatctgcttg cttgctgact tggagttcat ttacttcgct
    17041 acgggcataa tcaagtaaaa tagactggct gccaggacgt tctttgtaag tggtcaaatc
    17101 aataactggc ttactgagat acgtgtcatc ttgcggaatc agctgttctt tgttaggcac
    17161 tggcaaatat aacttatcta aaaaattttg acattctttc aaaaaaagcg gatcgccagc
    17221 ttgatattta acagctaagg ccacattata atgcgccaga tagttcgtat atgcattatt
    17281 gtcgatatat tctgtgtatt catctggacc aatgactctg tgtatctcta aatgatcatc
    17341 gacttttaca gctctgctca gccaaaaagc tgctgtttca tgaatgagcg tttttccctt
    17401 tgtttctaaa aacgattgat cctctgttgc ttggacatac tcttgaacgg cataagcaag
    17461 gtctgcactg atatgatgtt ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt
    17521 gatattcaat gcagcaaatt tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg
    17581 gaaaagtgca ccttgatagt gataatctct tgctttttgc cgtgcgcctt ctaatcgatc
    17641 ataacggtac agcagcaaat tctttgctac ttcaggaaac tgatataaaa agaaaggcag
    17701 gataaaaatt tccgtatccc agaaaacatg ccctttataa ccttcgccag ttagtccctt
    17761 cgcccctaca ctaacacttg gatcattgcg gggagtcata atctgtaaat ggtaagcagc
    17821 gaagtccatc gctaactgat cgaaagaaga atcgccttgg atcgttatcc gatgctgatg
    17881 ccaaaaatct gtccaataag acatcgactg ctctaaaatt tctgtataac taacgctttt
    17941 ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc tgaagagagg tagagagcac
    18001 gatgatcttc tccagcgtaa aggacgtatt ttttctgatc ttttctgaaa aggaagcggt
    18061 gatccggcga ttttttgctt ggaatacacc tgctttattg aatttagctg caatcccaat
    18121 atcaatttta ctttctgttg tctggtagga tgcactgatc aaatcgtcac caaatactcg
    18181 cagttctttt tcaaccaact gctgtgtacc aaaattggtt tgctgtccat cgatacccgt
    18241 tttcacatct acttggcagt cttcggtcaa cggcttgatg accactttca gggctgcctg
    18301 atggagatta ttttgatcag caacacgaca agtaatgact tccacttttt ttccttgagc
    18361 agtttcaatg agcgcatagc gtttaagctc tcccatttct aaatctagtg cccgctcgta
    18421 tgcaagaact ttcatcgtct caagagaaaa atgttcaccg tcgattttta tttcccatct
    18481 gatgacatct gggagattca ctaactcggc tgattctgct cctaaaggtc ggttatatac
    18541 accagctgca aacattcccc ttacttgatt ccgatagtct tcctcctggc ttcctctgat
    18601 accaaaaaat ccatttccaa tggtcatctg actcgcatac ttattgatat actctaaatc
    18661 aaactgtttt tcttctactt tcataaggta ataacttctc ctgtttctgc cgaacgatat
    18721 aaattttcca ccagattctg gatgacatat ccttgccagc catctgcaat catctgagtc
    18781 tggtcaccga gcgcccgatt cacaaaatga gccatactta gttcatgtgg gtcattaact
    18841 ggcgtggtct tttgtaagga aaccagcttt ccatctacat cttcgaatag ttcgcctgga
    18901 tacagattag cacctgcctg atcgccacaa aaagccacat tcagtttgtc ttctctgata
    18961 ttcaaagcaa acgaagtatt cagttggatg atcttgccgt tttctaattc cagtgtgcca
    19021 aaaattgcat cttctactgt gaatttgctt ggatcccatt tcccgaattt ccctgaattt
    19081 tttttcgtac caattttttg gaagctgtac gcccgaacgc tttttacttt aggaaatcca
    19141 gtaatataca tcgctgtatc aagcatgtga atgccataat cgatcaaagg cccgcctcct
    19201 tgaagttctt tgtcgataaa cgttccccag cctggaacac cagaccgacg aagcgcattc
    19261 gcttctacgt aataaatatc cccgagaaca tcaagatgat tcttgatcat tgttgcttcg
    19321 tcagaaaaac gatgctggaa tccataagcc aaaatctgcc cgtttttatc ggctgtctct
    19381 gccatctttc ttgcttgatc agctgtcata gctggtggtt tctcgcacat cactgaagca
    19441 ttagcttcga gggcagccaa tacatgttcg aagtgaaagc gattgggtgt acagacactc
    19501 acgatatcgg gagaaatctc attcagcatt tgtgtaactg atgtatacgc atgagaaaat
    19561 cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac agcggccaat
    19621 tctaagacat ctgatctttt ttgataataa gcagcatgta ctttttctgc tacttgtcca
    19681 gcgcctatga ttgctacttt cttcatttat ttctccctcc tttgcttatt tacttattgt
    19741 tattctttct ctattctttc tctaaatcat ttaaatgacg cgctgtttcc agacagtttt
    19801 tcaagtattg caatgattcc tgataaattt tcacttcatt ttctcctcgc aaacgacatt
    19861 catataccat ataaccttca tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat
    19921 caattgcgcc agatcctggt tggtagcgat gattatctgc taaatggata tgacccacat
    19981 actctttttg cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc atatgataaa
    20041 aatcagcgat gatctgcaca tgtttcaatt gattagagtc aatgtaatga cgcgcatccg
    20101 ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg ctccaaataa ataaccgtac
    20161 ctgtttcttc ggctacttta tctaaataac gaagtgattc gctgactgct ttatagtctc
    20221 cttctttgct tcggggtgaa accatcggcg gtaatcgata agtgaacatt ccccaagctg
    20281 ctgggataac gattcctttg cctccaacct cgcttagtgc acgcaaaatc tcagcaattt
    20341 gacgtacacc attaagacgc cgttcttcga taaagtcacc gatccaccca tcgtagccgt
    20401 tacaagctgt cactactggg atccctgttt ctttgattgc ctgtttgact gtgtcgatat
    20461 tttctattaa caacttaccg tcgatttcgt attcttcaaa tccacagtct ttgataaatc
    20521 taaatttttc taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg
    20581 ttctcctcct cggacttgaa cgcccatttt gatactttct tctgggtgct ggtctacata
    20641 acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact ggatcaatga tttcttcaca
    20701 atccaagtaa ccgttcatca ataattccca acatgtgtcc tcgattcgtt tacggttcca
    20761 tcttgggtaa tctgggtttg gttcactaga tgctcgagca aagacgattt ttgcattatt
    20821 gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa aggcaacata
    20881 agaaatgatc ccgccatagc cgatccctct taaagaagct tgtaatgcgg cagcactgcc
    20941 actcgtttca ataactgaat ctactccacg tttatctgtc aattccttta gacgaaaacc
    21001 gacatctgct ttcataggat ctagcgtgta atcggcaccg tatttttctg ctaattctct
    21061 acgctgcgct atgggatcga tcccaacgat ggtagtagca cctgcttttt tagctaattg
    21121 aatcgcaatt tgcccgatag cgcctaaacc gaagattgct acagcatcac ctgctcgaac
    21181 atgtgcttcc cggataccac ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt
    21241 ccattgatcc aaccgatcaa gtttacgtag tttataatta tcaacaccgt ttgtaatcag
    21301 cgtttcacga atgggtccat aagtgcagac taactctcct ttttgatact ctgtcaccaa
    21361 actgcccgtt tcttcgatcg taccgacaat catatttcct aattgaaatt cgccaaatac
    21421 cacaccgcgt tggcttcctt cttctcttgg caagaatagg cgccattctt ctgaaaattc
    21481 ttcatcgata aatggactta atcctctaaa gtcgacaatt tctgttcctg gttttggagt
    21541 agcgaaaatt actcggatac gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt
    21601 gaatccggct attcttggtt ctaatgcaac taattttttc atcttttctt tcctctttct
    21661 tttcttttta tccttttacg ccgccttcga ctaaatcacc tttaataaat cgttcagata
    21721 acgcatacat aatgatgact ggtaaagcgg tcaccaatga agcggccatc attcgtcccc
    21781 aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat tggtaaagta aatttttcgg
    21841 aggtcgacag gaaaatcgaa gcaaataaat aatcattcca cgaaaccata aagcaataaa
    21901 cgaagacaga aatgattcca gaaatagcca atggtataat gatccgaata atgatctgga
    21961 tccggctaag accatcgatc attgctgctt cttcaatatc agtcggaatc gtgtcaaaat
    22021 aactttttag catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg
    22081 ctgtattggt atcatacaaa cctagaccgg aaatgatttt aaataatgga acaacgatca
    22141 aaataccgga aaacatataa acggtgtaaa agctagcatt gattttcgag cgagctttga
    22201 agcgtagttt tgacaaagcg tatgccccgc tgatcccaat cactactgca gttacggagg
    22261 ctatcaagga gatcactaaa ctatttttga aatacatgat gaatggaaaa atctcagcat
    22321 tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg
    22381 caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat
    22441 tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc tttttatttt
    22501 tcatttcaag tccaccttct ttcgtgagag caagacgacc gctgcaataa tgatgaacaa
    22561 tagaatcgat atagctgctg cttttccaag atcattgaac gcaaaggctg tctgatacaa
    22621 ataaacgcct aagatattga ctttattcgt cagtagatag acatctgtaa acatatagaa
    22681 catccagata gatcgtaaaa caatcacagt tgctagtact ggtttgattg ccggcagtgt
    22741 cacgatcttg aacttctgcc aagcatttgc accgtccatt tcagctgctt cataaagcgt
    22801 tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat agtatctcca
    22861 gatagcaaac aaaacgacca taaagaagct agcaactggc tggtcgaacc acagcggtgc
    22921 ttcgttgaaa agctgcaacc tatctactaa cagatcattg acgattccat aactattatt
    22981 gaacatatat ttccaagcaa aaatcaaaga aatagatggc gtaacataac ttaataaaat
    23041 cagtgaacgc accgttttcc taaatctgaa cttgcggtta agcaaaatag ctacggctaa
    23101 tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga
    23161 acgataaaat tcactatcac ttaaaatttc aagataattg cttaatccga taaaaacggc
    23221 gtctgagtta ggattcagcg gcatatcgaa aaaactgatc tgaatattgg aaagcattgg
    23281 aatcagcact aaaacagcta ataaaagcat gcttggtgct aaaagtccca tggctaattt
    23341 taagtcagaa tgtttccttg gacgattcat tgaaacctcc ccctgataat ctgccaatag
    23401 ctatctactt ttttcgaaca ccattggcag aacttcttgt tatttcgtat ttttttgtgc
    23461 gtcagctaaa gcttgatcga catcttcgtt tccgacagtt acttggttca ctgcttttcc
    23521 gatcacacct gagcttgtga tatctcccat tttcgtgaaa ttcttttccc cgactagtcc
    23581 aaagacttgt acttcatcaa aagcactagc aatctcagat ggcagctcgc caaaagactt
    23641 gatgacttca ttttctttgt aaccatccag ttcgactact tgtttattca ctggttgagc
    23701 accaccagga gacatcaaga cccacttttc catgtttttc ggttgagata agaaagctgt
    23761 gaatgcttca gcagcctctt tttgtttctc gtctagacca gaagaaatcg tcaatccaga
    23821 tactgtgcca tagacagctt gatctttttg tgttgggata gcaaaaccga tattttctga
    23881 gttccccgct tcataaacag aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt
    23941 tccattcatg aaagcatctt tgatttctgt cacatcatta gagcctggca tcgtgtattg
    24001 ggataagttt tgataaaacg acaatgcttc tctcatcttt tccgtatcga tcgttacctc
    24061 gccttcatca tccaaaacat ttgctccatt tgacaaagca aattgagaga acgcttgctc
    24121 actcattgtt ccttcagcag taggcatagc aattccatat tgtttattct ctttatctgt
    24181 gaaatgttga gcaattttta aaatatcatc ccaattttct ggttctgaaa aaccagcttc
    24241 tgataatttt tctttgtcat accaaatacc ttgaacccag ccgctaattg gtgcagcaat
    24301 atagcttttt ccatcttctg aacgaacaag gtttttggct ccgtcataat aattatcttc
    24361 tccagcttcc tcgataacgt tttggactgc cgtctgatca atcagttcat ctttatccat
    24421 cactttagca aaatcctgac tgacttccat tacttctggt aatttaccgg aacgagctaa
    24481 cgtaacgatt tttgtattga acgctgattc ttctaccggc acttgcttga ttttgatgtc
    24541 tggattttct ttttcaaaat cagcaaccag ttcattgatc acacttaatc gttcctgttc
    24601 gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc tttgactttc
    24661 accacaagca gtaagaagaa atccagtagc tagcagacag gtagtaacaa taattttttt
    24721 catttttttc cacctcttat tttattattt tcagccacat atattcatac ggctggaata
    24781 tcaactttcg ttcttgactg caatggataa tttgtccaga aaacaggtct aggtaatctc
    24841 cttttggtaa aagataacct gctgattgat tggaaaaatt atggataact aagactttct
    24901 cttccttcgc ttcgcgaata aaactgataa aagaatctgc tgagtccagt aagtaaatca
    24961 aactattcgg atggaaggct ttttcttttt ttcgaataga tagcatctgg gtcaattcac
    25021 catatactgc tgttcttaat ggatctgtct gcaactcttt ctcaatatct gctaacaaaa
    25081 atttttgccg attgattttt cgattctctc ctgttttttc tacgcctttg taatcatttc
    25141 gacttcccag cacactttga atataaattg ccggcacgcc tggtaacgtt accaaaaatg
    25201 aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt atctgttgga gaacttaacg
    25261 catctagata cgtagcgttt atctcatatg gactggtcgt tccatctggg tttttcttgt
    25321 agtttactcg ggcaccctct ttcttcagct gttccactaa ttcaaggatc gttttttccg
    25381 gcagaatacc tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa
    25441 aagttgtttt tttcgatgcg aaatcgatgg tttttaacca ttctttcaac ttacttgtat
    25501 tacccgaacg aatcgcttga agggtcaaag gtggtagcga aaactgatag accatctgtg
    25561 cttcattctc gccattccca aaataactga tattatcttt gtgtggcaca tttgtctcag
    25621 tgatcaatac cgttcctgga gctgcttcat cagcgattgc acggaataat tggatgattt
    25681 tatgcgtttc tggcaggtgt atacaagaag ttcctagttc tttccataaa aatcccacag
    25741 cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa caaaagaata tcgatcattt
    25801 tcaataaaac ttctggattt ttaaaattca agtctacttg atcttcgcta aaagtcgtcc
    25861 atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg
    25921 gtctagtcac gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg
    25981 tatcattcaa gtttaaatat tcctgaaacc aagtactttc ggcagacata tgattgcaga
    26041 caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc tatatcttcc catgtgccca
    26101 aacggtcgtt gattgcctga taatttacaa cggagaaacc atcatcagat gtatatggga
    26161 aaaatggcaa gaaatgtaca actctaaatg tatctgccaa atacttttga taaaatttct
    26221 taaatgtacg caaagttggt tgctgctctt caaaaaattg atccccataa gtaatcaaga
    26281 aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc tgataaacac
    26341 tgactttttg ttcaatcata gaggcggttt tctcagacaa atctgagtcg cgataaatcg
    26401 cttgtaaaag ttttgtatac atcggattcc cctttctttt ttgggagcgt tcccaacgat
    26461 ttgatcatat tatggaaaac gcttactgtc aatagcattt ttttaaataa ttttgatata
    26521 cacaaaaata caaataagaa agcttatgta tcaataatta atagcttctt aaaaaaatat
    26581 ggtctctcat gatcatgcaa gctattccta aattttcaaa atcatagaca aaaaagaatg
    26641 tctattgtac tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga
    26701 aacctactat ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag
    26761 tattaaataa ccagccaaat atctctgata aagcaaaagc aagtgtactt caagcaatca
    26821 aagaattgaa ttatgaaccg agcaaaattg caagaggact gtcaggaaat ggttttgacg
    26881 cgattctagc attatctcat cgtacttctc atacgaccta tggcaatcct tttttttcag
    26941 atgttattca atccatttct gccgtttctg aacaaaataa cttcgacttg attctacaaa
    27001 cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga
    27061 tccgaggaat tattatttta agctctacga tggatgaaac acttcttttt gaattggatc
    27121 aatacaatat tcctattgtg gtaatcggaa aagtcggaaa cgactacacg aacatctact
    27181 cagtggacac ggataatttc caggatagtt acgatttggt ggaaacactg atcaagaatg
    27241 gacaccgtca tatcggctgt ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc
    27301 tcaatggtta ccgtcaatgc ttatttgatc accacctaga tatccgtaat gaatggatta
    27361 tagacggcgg ctttcgctta gaagatagca tggcagcaat gaatgattta atgaagctac
    27421 gagaaagacc aactgcgatt ttttctaccg atgcattaaa atcattgagt atttacaaag
    27481 tggcagaaca atctggaaag cagatacctg atgatctttc gatcgcagga ttcagtgatc
    27541 ccttattgtc ctccctattt tcgccacagt tgactcggat agacattccg acaaatcagt
    27601 taggaatcaa agccacagaa cttttattcc aactaatcca ccaccaatac cctgaagaaa
    27661 aaagaatcat tctttcaacc gagattactc tcacagattc gatccaacaa caagaaatgt
    27721 agaacagaat tataaaagaa cagaaacact gaggataagt cagttactct gttctttttt
    27781 caatcactca tccctcgtta ccccctcccg gtattttagc gaatttttct tattttccat
    27841 ctgttttaga tagctgtgga taactataat gtttgtgtta aactattttt tccacaggga
    27901 agaaaaaact atttttaacc acgttgtgta taagttttta tagttcgttt ttttaaacac
    27961 atttaccctt atttcaatcc atgttatttg gggatactta tctctaaaac ataaatagaa
    28021 taacagacat gtctactggt tttgttttat tcgaggaatt attcaataat agctagcaaa
    28081 atattaaaaa taaccgctct atcttcccat caatttattt tgcattcaaa aaatgatcta
    28141 tctttacttt tgttgattga cttctcttgg aagatgatcg ttccgatacc ttaattggat
    28201 acgaaccgat gttgttaaga tcctttccca cttttcttct agttagacaa attattttag
    28261 acaaaccaaa aaagaggttg tgacaataat ttgtcacaac ctctttttta aaatatctat
    28321 tttgtttgat tgttcattgc gtcttctgca gccatttgcg cttcaatatc tgaaatactg
    28381 tagacatttt cgcttgctac accgacttct tttggttcca tgtcacgata acgttgcata
    28441 ccagtaccag ctgggatgat cttacctata ataacatttt ctttcaaacc aagaagagga
    28501 tctttcttac cgcggatcgc agcatccgtc aagacacgag ttgtttcttg gaatgatgca
    28561 gcagataaga aactatttgt ttctaatgat gcttttgtga tacctagtaa gacaggtcga
    28621 gaagttgcag gaacgcctcc tgagactagc gtattgtagt tttgatcttt aaagtctgcg
    28681 atgtctaata atgtacctgg taagatatct gtatcacctg gatccattac acgaacttta
    28741 cgcagcattt gacgaaccat tacttcgata tgcttgtcgc cgatttctac cccttgcata
    28801 cggtaaacac gttgtacttc acgaagcaag tagttttcaa cagataatac atcgcgaact
    28861 tgcaacaatt gttttggatc gattgatcct tctgtcaatg gtgcaccacg gtggatcata
    28921 tcgccttctg ctactttcat acgtgctgta taaggaacag tatacgtacg tgtatctgtt
    28981 tttcctttga ttgttacttc tttggtacgt gttgcaggat cttctgagat atcgatgaca
    29041 tcccctgtta cttctgtgat cactgcttgc cctttcggat tacgtgcttc aaagatttct
    29101 tggacacgag gaagaccttg agtgatatcg tctccggcaa ccccacctgt atggaatgta
    29161 cgcatcgtca actgagtacc aggttcaccg attgattgag cggcgatcgt accaactgct
    29221 tctccgactt caacgtcaga gccagttgca aggttgcgtc cgtaacaatg tttgcagaca
    29281 ccatgttttg tattacatgt gaagacagaa cggattgtca ctgtttcgac acctgcatca
    29341 acgatttgtt ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat caattcattt
    29401 gtttcaggat gacgtacttc tttacgggtg taacgtccca ctaaacgatc ttctaatgat
    29461 tcgatgattt catttccctc gcggatggct tggatttcaa gacctcggtc agtaccgcaa
    29521 tcttcttcac ggatgattac atcttgggcc acgtcaacca gacgacgagt caagtaacct
    29581 gaatcggcag tcttcaaggc tgtatcggtc atccctttac gagccccgtg agttgagata
    29641 aacatttcca agacggatag tccttcacgg aagttcgaaa tgattggcaa ttccatgatt
    29701 cgaccattag gagcggccat caaaccacgc attccggcaa gctgtgtaaa gttggaaatg
    29761 ttaccacgag ctccagaatc agacatcatg aagattggat ttttcgcttc caaaccttcc
    29821 atcagttttt gttggatgct gtcttttgca gcattccaaa cagcaatgac acgttcataa
    29881 cgttcgtcat ctgtgatcag accacgacgg aattgttttg taatagtttc aacttgttta
    29941 tgggcatttt caatgatttc ttgtttttca ttcagtacac tgatgtcagc gatccctact
    30001 gtgataccgg catgtgtcga atgtttgtac cctaagtctt tcatacgg
//
LOCUS       DAYLZO010000018        29827 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.018, whole genome shotgun sequence.
ACCESSION   DAYLZO010000018 DAYLZO010000000
VERSION     DAYLZO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.018"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            155..1861
                     /gene="efbA"
                     /locus_tag="ACGVJG_000781"
     CDS             155..1861
                     /gene="efbA"
                     /locus_tag="ACGVJG_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFJ6556759.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="ACGVJG_000782"
     CDS             complement(2019..2921)
                     /locus_tag="ACGVJG_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFJ6556760.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3610)
                     /locus_tag="ACGVJG_000783"
     CDS             complement(2918..3610)
                     /locus_tag="ACGVJG_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFJ6556761.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="ACGVJG_000784"
     CDS             complement(3653..4294)
                     /locus_tag="ACGVJG_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFJ6556762.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4646)
                     /locus_tag="ACGVJG_000785"
     CDS             complement(4332..4646)
                     /locus_tag="ACGVJG_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556763.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4634..5263)
                     /gene="pyrE"
                     /locus_tag="ACGVJG_000786"
     CDS             complement(4634..5263)
                     /gene="pyrE"
                     /locus_tag="ACGVJG_000786"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFJ6556764.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="ACGVJG_000787"
     CDS             complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="ACGVJG_000787"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFJ6556765.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5983..6915)
                     /locus_tag="ACGVJG_000788"
     CDS             complement(5983..6915)
                     /locus_tag="ACGVJG_000788"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFJ6556766.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="ACGVJG_000789"
     CDS             complement(6912..7688)
                     /locus_tag="ACGVJG_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFJ6556767.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ACGVJG_000790"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ACGVJG_000790"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFJ6556768.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /locus_tag="ACGVJG_000791"
     CDS             complement(10932..12008)
                     /locus_tag="ACGVJG_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFJ6556769.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="ACGVJG_000792"
     CDS             complement(12013..13293)
                     /locus_tag="ACGVJG_000792"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFJ6556770.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="ACGVJG_000793"
     CDS             complement(13312..14238)
                     /locus_tag="ACGVJG_000793"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFJ6556771.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="ACGVJG_000794"
     CDS             complement(14251..15534)
                     /locus_tag="ACGVJG_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFJ6556772.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ACGVJG_000795"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ACGVJG_000795"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFJ6556773.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="ACGVJG_000796"
     CDS             complement(16322..17233)
                     /locus_tag="ACGVJG_000796"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFJ6556774.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /gene="lspA"
                     /locus_tag="ACGVJG_000797"
     CDS             complement(17230..17694)
                     /gene="lspA"
                     /locus_tag="ACGVJG_000797"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFJ6556775.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="ACGVJG_000798"
     CDS             complement(17699..18397)
                     /locus_tag="ACGVJG_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFJ6556776.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="ACGVJG_000799"
     CDS             complement(18384..18770)
                     /locus_tag="ACGVJG_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556777.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="ACGVJG_000800"
     CDS             complement(18917..20587)
                     /locus_tag="ACGVJG_000800"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFJ6556778.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20770..21003
                     /locus_tag="ACGVJG_000801"
     CDS             20770..21003
                     /locus_tag="ACGVJG_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFJ6556779.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21102..21533)
                     /locus_tag="ACGVJG_000802"
     CDS             complement(21102..21533)
                     /locus_tag="ACGVJG_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFJ6556780.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21612..22025
                     /locus_tag="ACGVJG_000803"
     CDS             21612..22025
                     /locus_tag="ACGVJG_000803"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFJ6556781.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22063..23211)
                     /locus_tag="ACGVJG_000804"
     CDS             complement(22063..23211)
                     /locus_tag="ACGVJG_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFJ6556782.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23333..23929)
                     /locus_tag="ACGVJG_000805"
     CDS             complement(23333..23929)
                     /locus_tag="ACGVJG_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFJ6556783.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="ACGVJG_000806"
     CDS             complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="ACGVJG_000806"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFJ6556784.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27159..27590
                     /locus_tag="ACGVJG_000807"
     CDS             27159..27590
                     /locus_tag="ACGVJG_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6556785.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27635..28984)
                     /locus_tag="ACGVJG_000808"
     CDS             complement(27635..28984)
                     /locus_tag="ACGVJG_000808"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFJ6556786.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29054..>29827)
                     /locus_tag="ACGVJG_000809"
     CDS             complement(29054..>29827)
                     /locus_tag="ACGVJG_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFJ6556787.1"
                     /translation="YFRLSFMKTMIASFCLSLFFELTQLSGLYFIYPRPYRLFDVNDL
                     VTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRLVAYLIDWTFLSLIN
                     LVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLGKKIVRIRLVEEDRE
                     RISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSAVFISLGVLLLQGIF
                     ILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
BASE COUNT         8435 a         6542 c         5255 g         9595 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttaaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg tttttaattt
    20701 atttgacgta cttataaata ctctttataa tgaaggctat gatcagactt tagaaaggtc
    20761 gtgagtgctt tgaccacagg aattgtaaaa tggtttgaca acaaaaaagg atacggcttt
    20821 atcagttatg atgacacaga agaaattttt gtacatttta ctgcgattga agaagaggga
    20881 ttcaaaactt tggaagaaaa tcaagtggtc gaatttgaga tcatcgaagg gaatcgagga
    20941 acacaagctg cccatgtcaa gaaaggcaac gttcagacta ccgatactag tatgcatgaa
    21001 tagataaaca aatagagtga gacgctaaac gggatgaacc gtacgtctca ctcttttctt
    21061 ttcctgtaaa aattcgtttt tttaggttct gatttttaaa attagctttt tctttttttc
    21121 gtataaggat aaggaattga ttctgatttg ggtccgtttt tttcaaaatg atcaaaaagg
    21181 actacttgat ctttttttcg catcatgaaa acagatgggg ctttctcatc ttgcaacatc
    21241 atttgcaccc cagtattagt tggcgtcaaa gatcgaatct gtccgacaga tatttctcgg
    21301 tctttccaaa aagcagcata acgtataatc acagagtttg attttaaaat aaaagaacgc
    21361 cgcaaaccta gaaatagaaa aacaagaaaa atacctaaaa caaaattgct tttccaataa
    21421 ggacctgtgt tttctaaaga aagaatcaga ctaaaaaaca gaatgatcaa ggtcaatgac
    21481 caatagatga tcgtatgtgc cagttcaggc tgccatcgaa tatttttttt cattctctaa
    21541 cctctcaccg attcagtaat gatataatct taccataaca ttagtttgaa caaaaaggag
    21601 aatctctttt tatgttaaaa gtatacattg atgcctctac aaaaggcaac cctggtccta
    21661 gtggaggtgg aatcttgatt gtctatcaag gtaaacaaga acagctatct atcccgcttt
    21721 ctatagggac aaaccatcaa gcagagttcg aagtcttttt aaaaacttta gaatttctaa
    21781 agaaaaacga cttacagaat gaaacgattt tctgtttttc agacagcaaa acactagttt
    21841 ctacggtcga caaaaacgca acgaagaacg agctcttcct tccctacctt caaagaatcc
    21901 aagaacttct atccgaattt tctttattga tcctgcaatg gttgcccgaa aacaaaaata
    21961 aaggagcaga taatcttgca cgtcaggctc tccataaaca attaaaagaa aaaaagactc
    22021 tttaaattaa aaactttaag gagtcttttt atagtttctc atttaatata aaagataatt
    22081 gtgatcaaac gtaatttcca attggtaatc tccgattttt tcttctattt gactacgtaa
    22141 gattctttct agttctgtat caacaaactg gaatttctca gggaccacta catcaaattg
    22201 cagcgttttt ccttgatgaa ttactctaaa atcatgaata ttaagccctg tacctgtttc
    22261 aagaagaatt tgctttaact gccgcaagat attatagtat ttttcgttat caacaggcat
    22321 tggatccaag tgacagacaa gttccacatc caaagacttt ttaaagtctt tttctatcag
    22381 atcaataatc ttatgagcca cattcaaatt cattgattca tctacttcga tgtggactga
    22441 tgcaaagcgt ttattgggtc catagttatg caccaataaa tcatgatagc caagtatatc
    22501 atcgcaagca tttaaatgct cttccatttc ttcaatctct gctggcgttg ggcgacttcc
    22561 taatagttcg tatacaaaat ttcgaagcat cgtcaatcca ctataaagga tatataatgc
    22621 taaagcaaaa cctatatatc catcaatacg ccatccagtt acccattcga ttgctgctga
    22681 aagcaataca gccagtgtag taaagacatc attataacta tctgttgctg tagctctcaa
    22741 tgtttcagaa tcaatggatt gcgcaataac ctgatacatt ttgccttgtg caaatttcaa
    22801 aaagatggaa atgattagga caaaaaagac gatgggtgtt aaagagacat tttctggatg
    22861 gatgattttt tcagcagaag actctaaaaa ttgaaatcct acataagtaa tgatgatcga
    22921 aacaaataac ccactgatat attcgaagcg ttcgtgtcca taaggatgtt cctgatctgc
    22981 tggctttgca gcaattttaa atccgatcaa agtcaatatg gatgaagcag tatctgatag
    23041 actgttcact gcatcagcca taatggagac acttcctgaa aaaagcccga tcattagctt
    23101 agagacaaac aagatcagat tcgtaaccaa tcctaagata cccgctagta ctcctttttt
    23161 tgtccgctgt tgcggtattt gtttcttttt ttcttcttgt ttcttagcca agttttttac
    23221 cccttctagt ttgcttgttg ctcaggtaaa aagatggaga ggacgattgt tttcctctcc
    23281 tctcttcctt tttctatcta tcgtgcgtcc tattactatt aaaagatggt cattattctg
    23341 gtttcaacgg tgttgctgca aataacgctt ttgctagtaa tggtgttatg attcccgcac
    23401 ctattgcttc tggtatccca ttgattgctg caacccctgc taaggtttta aataaaagag
    23461 cagagtctac accataggca tctgccgttg cacctgtata aaataatccc atgaatccta
    23521 acactaagac tgtattgacc aatgaaccca gtacccctgc aaaggctgag ctgatcgtta
    23581 ttgatttatt tttttgataa atccagtgga aaaccaagcc tgctactaag ccaacaagta
    23641 ctctgggaac taccgagata atcggattcg tgaacactaa tgtatcaaat ggcgttgttg
    23701 gcatggcata ggcacgaaca attgtagcga ttccccaaaa caagccaatc accatgccat
    23761 cttttgggcc taatactact gcagcaataa ttactgtgat atggataatt gttaaactaa
    23821 tgaatcctaa tggaataaac cccagccaag gaaccatcgc ttgtacgatg atgattgcta
    23881 acaagatcgc acgtaagact agtcgaaagg tcttatttct actattcatt ttttcacccc
    23941 tttttattta tactaatcgt aaaaatttct gtcactttcg tcaactaaag accatccgaa
    24001 tccgaccatt tgattagatg cgccatccaa ttgaaaaaag tatgagggca agacttgttt
    24061 gtcttcgccc ccataatcat gtttcgttca atgtttgaac ttttgttttt ctgtcaaacg
    24121 taaacgccct attgttgcgc aacccattct ttcgcagctt taagtgcatc tggaatacca
    24181 gcaggatttt tcccgccagc ttgtgccatg tctggacgtc caccaccgcc accgcctact
    24241 ttcggtgcga ttgctttaat caagtcgcca gcttttagtc cagcttcatt tgattctttc
    24301 gtcattgccg ctagcagact gacttttcct tcttgttcgt ttgctaaaac aagaatatct
    24361 gaaatatttt tttgtttcca ttggtcagct aattgacgca gctgattcat atctttcact
    24421 tttacttgtg ctgcaatgat tgtttttcca ttgatcgttt ctggatcttt gaatacatca
    24481 ccagcttgtt gattcgctaa cttgctagct aattgttcat tttccttttg tagctctctt
    24541 acttgctgtt gcaattgttc cgcttttgaa acaacttctt tcaattgagg tgatttaaca
    24601 attgttgcta cttgttttag ttgtttttct tcttcttcaa gtagttcgta cgcttcttta
    24661 cttgttacag cctcgattcg tcgcacgcct gcaccaattc ctgattcaga aacgattttg
    24721 aagattccga tgttttctgt atttgctaca tgtgtgcctc cgcacagttc gattgaccag
    24781 ccgccaatat ttactactcg aacgtcatgt ccgtattttt caccaaacaa agccattgca
    24841 cccatgtttt tggctgtatc gatatctgtt tcaattgttt caactgggat agcttcccag
    24901 attttttcgt tgacgattct ttccatttca gctaattcat ctgaagtgat ttgaccaaag
    24961 tgggtaaagt cgaatcgtaa atgtccagga gcgactaaag aaccagcttg atttgcatgc
    25021 tcgccaagta cttctttcaa tgccttgtgc agtagatggg ttgctgtatg gtttttgatg
    25081 atccgattgc gcatcgattg atcgatcaat agatgatagg tttgtccttc actcattggt
    25141 gcttttactt ctacggtgtg cataaattga ccattaggcg ctttttgaac atctgtaaca
    25201 actgctacga tttcgcctac ttcattcatg atccatcctt gatccgctac ttgcccaccc
    25261 atttctgcat agaaaggtgt ttgatcaaag atcatttgag cttcaccttc tgcgacttca
    25321 ttcaaaagag actcaccttt aacgatgacc aataattgac tatctgattc ttgttctgtg
    25381 tatccaatga atttactttc tactttgata tctgtcaata aagcagattg tacccccatt
    25441 gatttttcag tactgcgggc agagcgagca cgttcgcgtt gtgcatccat ttctttttca
    25501 aaaccagcat gatcaacttt cagtccttca tcttctgcca cctcttccgt taattctacc
    25561 gggaagccat atgtgtcgta tagtttgaag atgtcttttc ctgctagagt cgtcccgttt
    25621 tcgtttttta cttcttggat cacttgattt aagatttcca aaccttcatt gattgtttca
    25681 ttgaaacgtt cttcttctgt acggataact ttttcaataa attctttttg ttgaagtact
    25741 tcaggataat aacttaccat gatttcccca acaacaggga ctaaacgata taagaatgct
    25801 tgttcaatac ctaatttttt cccatgcatg acagcacgac gaagcaaacg gcgtaagaca
    25861 taccctctcc cttcattcga tggcaatgca ccgtcgccaa tagcaaatga aagggcgcga
    25921 atatgatctg cgattacttt gtaagaaata tctgttactg ggttttcacc atattttact
    25981 tgtttgctta gcttttctac ggcgtggata ataggcataa acaagtctgt ctcaaagttc
    26041 gttgacgcat cttgaacgat tgagaccatg cgttccaatc ccatccccgt atcaatgttt
    26101 ttatgaggaa gtggttcaaa tgtgttatca ggcttatggt tgaattctga aaatactaag
    26161 ttccagattt ctaaataacg ttcattttct cctcctggat agttttcagg atcgtcttct
    26221 gctaaatcgt tataagcttc tccacggtca tagaaaattt ccgtatccgg accacatggg
    26281 cctgcaccga tatcccagaa gttgtcttct acatcaataa tgtgatctag tggcaggcca
    26341 acttcttcat gccagatgcg ttttgcttct gtatcttttg gataaactgt tacataaagt
    26401 ttttctggat cgaatgccat ccattttgga tcagtcaaaa attcccatgc ccagtggatg
    26461 gcttcatttt tgaaatagtc accaatcgaa aaattaccca gcatctcaaa catcgtatgg
    26521 tgccgtgctg ttttaccgac attttcgata tcatttgttc gaatagattt ttgggcattt
    26581 gtgattcttg gattttctgg cacaactgaa ccatcaaaat attttttcaa tgttgcaaca
    26641 ccagagttga tccacaataa ggttggatcg ttcacgggga tcaaagacgc actaggttca
    26701 atcgtatgtc ctttagattg gaagaaatca agaaacattt gtcgaacttg tgcactactt
    26761 aattgtttca tgtcattacc tgctttctat aaattaaata caaaaaaaca ccatcacagc
    26821 caaggacgaa aactcgcggt accaccttga ttgcaatgat gtgtaatcat tacctcttaa
    26881 actcgataac gctgagctgc gtcggtattt ctaccaaaat agaaaaaggg agcaatttga
    26941 ttagtgacta ttcttttttc agccaagaaa gaattttctg tgagtcttaa aaaacaaatc
    27001 atatcctttt cgtacttaat tagtatacgc attctattga tcactgtcaa tatctcaatt
    27061 tttattttca tttccttttg gtcatactcc acgatttttg gtacgataag aataaaggaa
    27121 tcaatacatc tctgctattc atgacaagga ggaatatgat gaaaaaaata gttggagcgg
    27181 atgaatggat acgcgcagct agctgctttt tgagatatca agtttttgta ttagaacaag
    27241 ggattcctgc agaactagaa ttcgacggaa aagatatccc gcaacaaacc tattatctat
    27301 ttcttgatca aaaaagacca gtagctactt tgcgttatga aaagaaaaca cctacttgct
    27361 tcaagccgga acgattttgc gtagcaaaag aataccgagg aaaagggtta ggcggtcagt
    27421 tgcttgaagc tgctgaaaaa cgggcggttt cagaaggttg tcagactagt tatgtagtag
    27481 ctgaaatgac ggctgtagat ttttatgaaa aacaagggta tcatacttgc acagaacctt
    27541 ttattgaaga tgaagtactt tgtatcggaa tgaaaaaaag tctagcttaa aaacaagaga
    27601 ttgtgacacg ccttttgtca caacctcttg ttttttattt atgtgaattt ttcttatttt
    27661 tacgagcagt acggctttgc tgtctgcgtt cgattttgcg ttctttttta tttttttctg
    27721 caattgccca atcgattttt ttcttataac caggcttgat ctttttcttt ttcttcttca
    27781 caagaccaat cagcgtggga tctaatacat cttttgattt ttctcgtttg gcacgtctat
    27841 tacgatcgta tgttttgacg atttccccgt ttttgatttc ttttggttca aatgagacgc
    27901 ccaatttttc tatcgcatca atggcttgct catcatttgg cgaatataac gtaatggccg
    27961 ttccttttaa gccattacgt ccagtacgtc cgacacgatg gataaagaag tctagatctt
    28021 ctggaatttc tgcattaata acatgagaga caccttcgat atcgatccct ctagcagcta
    28081 aatcagtcgc tacaacatat tgatactcca gattctgtac ttgtcgcatc actcgttttc
    28141 tctctcttgg ggtgatgtct ccatgaatct ttgctacttt caagccttgg tttttcaagt
    28201 aatcggcaat ttcatccaca tgctgttttg tattggcaaa tacgattgct aaataaggat
    28261 gaccaattgt cagcagttga taaatcattt gattctcatc tttacctttt gttgaaatca
    28321 gccaattttc aatgtcttca gaaataactg cttttggttt gatttcttca atgattggat
    28381 tttccatgta ttttcgtaag aatggacgaa gtttttctgg aatagtggca gaaaaaacta
    28441 aaaactgcag cttttctggt aatcgacttg caatttggtc tacttcattt aaaaaaccca
    28501 tatccaatgt catatccgct tcatcgacaa caaatgcaaa tgcggtgtga atcttcagcg
    28561 cttgttcatt catcatgtct aatattctac caggcgtgcc tactacaacc tgtggctgtt
    28621 ggtgatgcag acgatttaat tgacgctgtt tgtctgtacc gccgacaaaa ttcgagacac
    28681 ggatctccgg tgtagaaaat ttagcgattt gtgtagctgc ttcatagatc tgtgcagcta
    28741 gttccctact tggagctgta ataacaactt gtacttcctc tttttctgca ttgatttttt
    28801 caaacaaagg caacaaaaac gtatgtgttt ttcctgtacc agtttgagat tgcccaatga
    28861 tattttttcc ttttgcaatt aaaggaatca aacgttcctg gacctctgtg ggctgtttga
    28921 agccctttgc ggttaacgct tcattgataa acggtttgaa gcggaatata tcaaaattat
    28981 tcattcgtgc acctcttctc tttaaattat cacgactttg gcattataac acagacaaag
    29041 cgtttttgct cagctaattt cgagaatcat catcctttgt ttggccaatc gtgctaacag
    29101 tatatgtgtg actagctttt tcataaaaca gaatccgttt ttttctcaaa aatgatagca
    29161 agatatttaa aataaaaata ccttgtagta acaagacacc gagagaaatg aatactgcac
    29221 tcatctgcaa caccgtattc gttgtcgaat tcatgatttg ctgaagcaac tgccccaacc
    29281 agcttaaacc ataaaaaaga aagtacagat acccgtagcg gatcatcaat gcttttaaag
    29341 aaattcgttc gcggtcttct tccaccaaac ggatacgcac aatttttttg ccaagtgtcc
    29401 atccattggt tataaacggt acaatcataa agtatacccc tacttctaat aaaagccacc
    29461 agatatgaga agtaactgcc cctgaatcga acgcaatttt aaagaaaatc gccgctacca
    29521 aattaataag agataaaaat gtccagtcga tcaaataagc gaccaagcgt cgagtcaatg
    29581 agacagtctt tcctttttca taagaaagtt catccattcg tcttcttgta ggaagcataa
    29641 atgtaaataa cggcgtaacg acaaaaccaa tgattccacc caatgtattt gtcactaaat
    29701 cattcacatc aaataagcgg tatggtcttg ggtagataaa ataaagtcca cttagctgcg
    29761 tcaattcaaa aaatagagaa aggcagaaac tagcaatcat cgttttcata aaagaaagcc
    29821 ggaaata
//
LOCUS       DAYLZO010000019        29099 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.019, whole genome shotgun sequence.
ACCESSION   DAYLZO010000019 DAYLZO010000000
VERSION     DAYLZO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.019"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..1031)
                     /locus_tag="ACGVJG_000810"
     CDS             complement(<1..1031)
                     /locus_tag="ACGVJG_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFJ6556788.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACGVJG_000811"
     CDS             complement(1113..1802)
                     /locus_tag="ACGVJG_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFJ6556789.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACGVJG_000812"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACGVJG_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFJ6556790.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACGVJG_000813"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACGVJG_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFJ6556791.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACGVJG_000814"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACGVJG_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFJ6556792.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACGVJG_000815"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACGVJG_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFJ6556793.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACGVJG_000816"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACGVJG_000816"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFJ6556794.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACGVJG_000817"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACGVJG_000817"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFJ6556795.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACGVJG_000818"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACGVJG_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACGVJG_000819"
     CDS             complement(7636..8523)
                     /locus_tag="ACGVJG_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFJ6556796.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACGVJG_000820"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACGVJG_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFJ6556797.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACGVJG_000821"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACGVJG_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFJ6556798.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACGVJG_000822"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACGVJG_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFJ6556799.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACGVJG_000823"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACGVJG_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFJ6556800.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACGVJG_000824"
     CDS             complement(10454..10957)
                     /locus_tag="ACGVJG_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFJ6556801.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACGVJG_000825"
     CDS             11554..12519
                     /locus_tag="ACGVJG_000825"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFJ6556802.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACGVJG_000826"
     CDS             complement(12618..13790)
                     /locus_tag="ACGVJG_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6556803.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACGVJG_000827"
     CDS             complement(13777..14922)
                     /locus_tag="ACGVJG_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6556804.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACGVJG_000828"
     CDS             complement(14936..15868)
                     /locus_tag="ACGVJG_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556805.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACGVJG_000829"
     CDS             15985..17064
                     /locus_tag="ACGVJG_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFJ6556806.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACGVJG_000830"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACGVJG_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACGVJG_000831"
     CDS             complement(17789..18016)
                     /locus_tag="ACGVJG_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFJ6556807.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACGVJG_000832"
     CDS             complement(18119..20707)
                     /locus_tag="ACGVJG_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFJ6556808.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACGVJG_000833"
     CDS             complement(20727..21386)
                     /locus_tag="ACGVJG_000833"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFJ6556809.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACGVJG_000834"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACGVJG_000834"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFJ6556810.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACGVJG_000835"
     CDS             complement(22159..23025)
                     /locus_tag="ACGVJG_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFJ6556811.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACGVJG_000836"
     CDS             23134..23775
                     /locus_tag="ACGVJG_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFJ6556812.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACGVJG_000837"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACGVJG_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFJ6556813.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACGVJG_000838"
     CDS             complement(25216..26115)
                     /locus_tag="ACGVJG_000838"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFJ6556814.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACGVJG_000839"
     CDS             complement(26117..26914)
                     /locus_tag="ACGVJG_000839"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFJ6556815.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACGVJG_000840"
     CDS             complement(26892..27596)
                     /locus_tag="ACGVJG_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFJ6556816.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACGVJG_000841"
     CDS             27727..28302
                     /locus_tag="ACGVJG_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFJ6556817.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACGVJG_000842"
     CDS             28420..29097
                     /locus_tag="ACGVJG_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFJ6556818.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8563 a         6167 c         5231 g         9138 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaa
//
LOCUS       DAYLZO010000020        28881 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.020, whole genome shotgun sequence.
ACCESSION   DAYLZO010000020 DAYLZO010000000
VERSION     DAYLZO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.020"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..578)
                     /locus_tag="ACGVJG_000843"
     CDS             complement(<1..578)
                     /locus_tag="ACGVJG_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFJ6556819.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLK"
     gene            complement(579..1466)
                     /gene="hslO"
                     /locus_tag="ACGVJG_000844"
     CDS             complement(579..1466)
                     /gene="hslO"
                     /locus_tag="ACGVJG_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFJ6556820.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(1683..3794)
                     /gene="ftsH"
                     /locus_tag="ACGVJG_000845"
     CDS             complement(1683..3794)
                     /gene="ftsH"
                     /locus_tag="ACGVJG_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFJ6556821.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(3884..4429)
                     /gene="hpt"
                     /locus_tag="ACGVJG_000846"
     CDS             complement(3884..4429)
                     /gene="hpt"
                     /locus_tag="ACGVJG_000846"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFJ6556822.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(4429..5874)
                     /gene="tilS"
                     /locus_tag="ACGVJG_000847"
     CDS             complement(4429..5874)
                     /gene="tilS"
                     /locus_tag="ACGVJG_000847"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFJ6556823.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(5994..6464)
                     /locus_tag="ACGVJG_000848"
     CDS             complement(5994..6464)
                     /locus_tag="ACGVJG_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFJ6556824.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(6510..6935)
                     /locus_tag="ACGVJG_000849"
     CDS             complement(6510..6935)
                     /locus_tag="ACGVJG_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFJ6556825.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(7002..7271)
                     /locus_tag="ACGVJG_000850"
     CDS             complement(7002..7271)
                     /locus_tag="ACGVJG_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFJ6556826.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(7282..8877)
                     /locus_tag="ACGVJG_000851"
     CDS             complement(7282..8877)
                     /locus_tag="ACGVJG_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFJ6556827.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(8892..12413)
                     /gene="mfd"
                     /locus_tag="ACGVJG_000852"
     CDS             complement(8892..12413)
                     /gene="mfd"
                     /locus_tag="ACGVJG_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFJ6556828.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(12430..12990)
                     /gene="pth"
                     /locus_tag="ACGVJG_000853"
     CDS             complement(12430..12990)
                     /gene="pth"
                     /locus_tag="ACGVJG_000853"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFJ6556829.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            13343..14317
                     /locus_tag="ACGVJG_000854"
     CDS             13343..14317
                     /locus_tag="ACGVJG_000854"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFJ6556830.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            14487..15887
                     /locus_tag="ACGVJG_000855"
     CDS             14487..15887
                     /locus_tag="ACGVJG_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFJ6556831.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(15965..16687)
                     /locus_tag="ACGVJG_000856"
     CDS             complement(15965..16687)
                     /locus_tag="ACGVJG_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6556832.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(16766..17272)
                     /locus_tag="ACGVJG_000857"
     CDS             complement(16766..17272)
                     /locus_tag="ACGVJG_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFJ6556833.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            17395..19194
                     /locus_tag="ACGVJG_000858"
     CDS             17395..19194
                     /locus_tag="ACGVJG_000858"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFJ6556834.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(19262..20836)
                     /locus_tag="ACGVJG_000859"
     CDS             complement(19262..20836)
                     /locus_tag="ACGVJG_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFJ6556835.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(21091..21600)
                     /gene="mreD"
                     /locus_tag="ACGVJG_000860"
     CDS             complement(21091..21600)
                     /gene="mreD"
                     /locus_tag="ACGVJG_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFJ6556836.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(21604..22458)
                     /gene="mreC"
                     /locus_tag="ACGVJG_000861"
     CDS             complement(21604..22458)
                     /gene="mreC"
                     /locus_tag="ACGVJG_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFJ6556837.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(22613..23251)
                     /locus_tag="ACGVJG_000862"
     CDS             complement(22613..23251)
                     /locus_tag="ACGVJG_000862"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFJ6556838.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(23252..23902)
                     /gene="rpe"
                     /locus_tag="ACGVJG_000863"
     CDS             complement(23252..23902)
                     /gene="rpe"
                     /locus_tag="ACGVJG_000863"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFJ6556839.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(23915..24814)
                     /gene="rsgA"
                     /locus_tag="ACGVJG_000864"
     CDS             complement(23915..24814)
                     /gene="rsgA"
                     /locus_tag="ACGVJG_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFJ6556840.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(24979..27048)
                     /gene="pknB"
                     /locus_tag="ACGVJG_000865"
     CDS             complement(24979..27048)
                     /gene="pknB"
                     /locus_tag="ACGVJG_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFJ6556841.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(27045..27785)
                     /locus_tag="ACGVJG_000866"
     CDS             complement(27045..27785)
                     /locus_tag="ACGVJG_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFJ6556842.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(27798..>28881)
                     /gene="rsmB"
                     /locus_tag="ACGVJG_000867"
     CDS             complement(27798..>28881)
                     /gene="rsmB"
                     /locus_tag="ACGVJG_000867"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFJ6556843.1"
                     /translation="QLEFLDKVPAHAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQG
                     APDLQGIADPIERLSVKISLPLWMTKRLVDQIGYEETEKLGLSLYQPSHASARVDTRR
                     LSRDQAITVLQEEEITAEKSQVSPYGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPS
                     MQIEPEHHVLDACAAPGGKTTHIASFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVS
                     SAVYAQKMDAREVSKQFEAEQFDRILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKI
                     QLEILESCAPTLKSSGILTYSTCTILKEENQDVVSAFLERHPDFERIDVLTNPMLQSS
                     IEEKMLTLYPHQFYTDGFFICCLRKK"
BASE COUNT         7931 a         6288 c         5308 g         9354 t
ORIGIN      
        1 tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt acgtccatgc
       61 atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac agcaaagacg
      121 tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac tgctgaagaa
      181 acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc tcctgcttct
      241 gctttgatta ctttatttac tggacagccc atattgatat ctattatatc ggcagctgtg
      301 ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc aaatatctgt
      361 aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt tttgtttctg
      421 aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc aaattctttg
      481 acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac gacacgatta
      541 gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc taaggcttct
      601 ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca gaaagaacaa
      661 accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc acctaacgcg
      721 ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac aggcattttt
      781 tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc tggtgtctgt
      841 ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc gatcttgctg
      901 attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc cgtcttgatg
      961 ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg ttctgagata
     1021 gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt ttgcccagaa
     1081 aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt tccaactgct
     1141 ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg atttttgatg
     1201 taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc tggaccatcg
     1261 ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag tagcagacta
     1321 ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg acgtctttgt
     1381 gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc gtcgtgagca
     1441 agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt tttaaactca
     1501 tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct tttaaaaata
     1561 attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata aagaaatctc
     1621 ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg acctatattt
     1681 ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc tgaatcatgt
     1741 tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg tttttcttcc
     1801 gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa agttttcgct
     1861 tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc gtgttcaaac
     1921 aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc agcaatcagt
     1981 ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc ttccatcaag
     2041 atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc ttttgtttga
     2101 ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg acctaaacga
     2161 tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg ctcaaagtca
     2221 ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg tccaccaagt
     2281 aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc ttcttttggt
     2341 aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt gtggacgacc
     2401 cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata agcaaccatt
     2461 tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat cacgcgatct
     2521 tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc agctactaac
     2581 gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt ctgttgagcc
     2641 acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac tcgcaagatt
     2701 gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa acggcctgga
     2761 cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac gattactcct
     2821 tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg ttcgcgttca
     2881 tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat ttcatcaatg
     2941 aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac acgacttgct
     3001 ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa cggtactcca
     3061 gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg tccttctaaa
     3121 agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc tttcagaaat
     3181 tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc agaaaaacgt
     3241 acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt catgacacgg
     3301 ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa aaagatgatg
     3361 attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct tgattcttct
     3421 tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct caatgttgta
     3481 tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga acctaagata
     3541 gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt gatcttatat
     3601 acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag ttgttctgtg
     3661 aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat gaaataaacg
     3721 accattacca tcgctaagat tacgaggaca taatacaagg catttttcat catgccgttg
     3781 ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt tttttatcaa
     3841 tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt gattgataaa
     3901 cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca taatccaagc
     3961 cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct tcgatattta
     4021 ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt gctttgcggt
     4081 atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata tcttcaacga
     4141 tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt acttcccctg
     4201 acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc aaatacgtat
     4261 cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca cctactacta
     4321 ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg caacgaactt
     4381 gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc aggttctcct
     4441 tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat cagtttcttc
     4501 acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc ttttggcatc
     4561 tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga taaaatatct
     4621 agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt ttcttttttc
     4681 gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag accatgaatc
     4741 tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt ctgtttcatt
     4801 caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct ggctagttgc
     4861 ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta tccaattatt
     4921 gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa tcgactccat
     4981 ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa aggagtaaaa
     5041 aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa atcgaaattg
     5101 ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt gaatgacttg
     5161 gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct gtgtattttc
     5221 ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat ctaacaattg
     5281 gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat ataggtcttc
     5341 tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt gtacttcttt
     5401 gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca ccgtctcgat
     5461 ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt ccatcacgcg
     5521 agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt tggctggatc
     5581 ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc gaagataggc
     5641 agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac ctaaagaaaa
     5701 accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat caacaccacc
     5761 tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat tcccgtgccg
     5821 aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt tcatcctttc
     5881 cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac aattaacctg
     5941 ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc tttttagctg
     6001 cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga agataagcgg
     6061 tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt ttgagctgaa
     6121 ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc acggggatct
     6181 ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg ttcctgcgct
     6241 ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt aactgttact
     6301 tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga cacttcgcta
     6361 atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa atttgtgatt
     6421 cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa aatgttcctc
     6481 ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact agttgattga
     6541 tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac tttttcaccg
     6601 tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc atctttcagc
     6661 aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat ttcagtattt
     6721 gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa aatctgcaca
     6781 cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct gcgtcttcga
     6841 aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc atattccgta
     6901 tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa tctttttgat
     6961 tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc tagtgtttct
     7021 gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt ggttgattca
     7081 tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt caaaatatcg
     7141 cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat tcttccttta
     7201 tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt taaaaattta
     7261 tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca ggattttctt
     7321 accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa tcactgcata
     7381 aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag cagtggaacg
     7441 atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca gtactactgt
     7501 catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc aaaaagcatt
     7561 tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc ctaaaagggt
     7621 agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag tcaatccttt
     7681 taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct tgctttgagc
     7741 aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa ataatacaag
     7801 cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga gcagcaaggc
     7861 taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga ttttggctgt
     7921 ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag gcaggaatgt
     7981 cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac ctcgatcata
     8041 gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca aagcattttt
     8101 tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa tcgaaaccag
     8161 tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga acaatggaaa
     8221 ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa ggatcccata
     8281 tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt ggtacaccgt
     8341 ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac gaacgaactg
     8401 ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga aattccctcg
     8461 ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga tcagcggttt
     8521 caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga caaacaacca
     8581 caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt gctttcgctc
     8641 gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac cggataaagc
     8701 taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa aaccttcgtc
     8761 gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg ctatgaaaga
     8821 agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat gatccaaatc
     8881 attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac aaacttttgg
     8941 atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg tatcaccatc
     9001 tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc ttcgaataac
     9061 tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat atgcgcctcc
     9121 tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg cccaattgtc
     9181 aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc atcttctagt
     9241 tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc gattttctgc
     9301 cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc gatctcaaca
     9361 gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc gcttagcatt
     9421 tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc acctaagaga
     9481 ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga tcccagttct
     9541 gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat cttctgctgc
     9601 tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc tcgcaattgg
     9661 tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt tgcgttcggg
     9721 atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg accttcaata
     9781 aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc ataaccgatc
     9841 cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt ttctacccga
     9901 ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc ttcgcgaata
     9961 gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc aggcggtgtt
    10021 tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct cggaatcggt
    10081 gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg ttccttatgt
    10141 tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct aaattcaata
    10201 tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt acgcagttgt
    10261 tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc gatatttacg
    10321 gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa gatcgttgtt
    10381 ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc tcgaagcgct
    10441 acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg tttttctttt
    10501 tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc agagtagggg
    10561 aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata tcctttttct
    10621 gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat ttttgaagag
    10681 acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg aggcgctttg
    10741 gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat aaacagtttg
    10801 tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac ttctagcgtt
    10861 tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc tcctgctttt
    10921 aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct cgtttgtttt
    10981 ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc ttgaggaagt
    11041 tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat caatgcattt
    11101 ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc tttgcgtatt
    11161 ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca acggtccatt
    11221 tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata ttgaaaatta
    11281 tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa aaaggttgtg
    11341 gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg ctcagaaaac
    11401 acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc gatttcccga
    11461 tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga agggaaataa
    11521 tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc ttcttctgtc
    11581 gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa aaactcgcgg
    11641 tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat ttgctctatt
    11701 ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac ccagatttct
    11761 tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga atctatctct
    11821 acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat atcgataatg
    11881 cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac atagcccatc
    11941 aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg ccaattcaat
    12001 tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc gactggcaac
    12061 aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct ctcgcctcgc
    12121 gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg aaataaatgg
    12181 atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc ataatacaaa
    12241 tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc aaatagaagt
    12301 gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc ttctaaattg
    12361 ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc cattcctttt
    12421 cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt ctatgagata
    12481 ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat tttctttgct
    12541 gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc cgattcccac
    12601 cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga taccgttatg
    12661 cccgccggca cttccctttt gacggagccg gatttttccc actgccagat ccaaatcatc
    12721 gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg ggccgactgc
    12781 acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt ctccattatg
    12841 aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt gatgctcgcg
    12901 tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt atttggtacc
    12961 tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc taaaaagcag
    13021 cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc ttttttacac
    13081 ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa tcgttaataa
    13141 atgataaaga aattaaaaat ttcttcaaag tttaatgaaa taattgatat ataaaaatag
    13201 tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg ttatatttta
    13261 tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg aaaaaacaaa
    13321 tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc atcaaaaagt
    13381 tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag ttactcaaaa
    13441 tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag gagacgcaat
    13501 tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg ctacttacgc
    13561 agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa aaccaggtga
    13621 aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg tcacacaaat
    13681 cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag atattttgac
    13741 ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt caggtacctc
    13801 tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg atgcacgtaa
    13861 cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt ggtcacatgc
    13921 aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa tcgatgaaga
    13981 agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta tcgagaaaaa
    14041 aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg ccatcttaga
    14101 tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg gattgaacga
    14161 catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag taatcgaaat
    14221 tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat taaaagaaat
    14281 tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga cggaaaacat
    14341 ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa ttttttcata
    14401 aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc gttccaccct
    14461 aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa aaatcgaaac
    14521 atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt tatacctatc
    14581 gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga ataaatgtca
    14641 gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct gataaaaaac
    14701 tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag ctcggctaca
    14761 aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga tggacttggg
    14821 gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt caaaaccacc
    14881 aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg aacaaagacc
    14941 ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt atcaagcctg
    15001 aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa agtgcggtaa
    15061 actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa gtaacatctg
    15121 aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat gtaaaagcta
    15181 cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg agttggctga
    15241 cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat gtaacagatt
    15301 taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca aaaagaggag
    15361 aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt gagacagaag
    15421 cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt gtgcaaggtg
    15481 gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat ctggagaacc
    15541 agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc gtttccggaa
    15601 agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa gaagatgcaa
    15661 ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac tactggatgc
    15721 cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa tacggtggag
    15781 acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt gtaatgaagg
    15841 aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa aaataatata
    15901 taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc taacaatgtg
    15961 ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct tctagcttat
    16021 ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc gtttgtggaa
    16081 tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct cgtacagcag
    16141 caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc agatcttctg
    16201 cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac ccatcgcgtt
    16261 caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt tctcccagtg
    16321 atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta cggttatctg
    16381 tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac gtgatttcat
    16441 cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc acattgtcgt
    16501 taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga ttcatagatg
    16561 gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc tgaaagactt
    16621 tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat ttactatgtc
    16681 ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta gtactatttc
    16741 acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca aaaagcacac
    16801 ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg aacataggtg
    16861 ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct gttgctgaaa
    16921 gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca cctaaaaaca
    16981 agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa aagaactcta
    17041 aaaatttggc gtagcccaga tggttgatac tcaccatact tccaccataa ttaaaacgta
    17101 tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat cgcgaaactt
    17161 gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg actatcagtg
    17221 cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc ataagtcacc
    17281 tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata gagacttctt
    17341 ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt tttcatgagt
    17401 tattcattaa attggaatct agattctatt ttttccggag gcagtcattc tgatgctttg
    17461 aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg cgtaacaaaa
    17521 tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt gcaagaaacg
    17581 attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc tgccaatgtc
    17641 aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc tcgtctgcaa
    17701 tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga ttggaatcag
    17761 atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat tcgtcgtgat
    17821 ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc tttagatggt
    17881 ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat tcccttcgaa
    17941 caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat gatgggcgat
    18001 ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg gaaagaaaag
    18061 gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc tgactatgaa
    18121 cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt gaagaaagaa
    18181 acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt taattacttg
    18241 actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca agatgcacct
    18301 attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc agcatttatt
    18361 atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc tgcttttgaa
    18421 aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta ttgtactgag
    18481 ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt taatgaggtt
    18541 gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg ggatttacct
    18601 tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt cgcagaactg
    18661 atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat caatttgcta
    18721 gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg attcattttt
    18781 gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa gatcacagag
    18841 atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta ccatccatat
    18901 ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa cttcccatac
    18961 acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa gggagcaaat
    19021 tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac ggaagatctt
    19081 gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc aggtatcgac
    19141 atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat ctgatttcta
    19201 ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg attaatcatt
    19261 cttacatgct gacagcaaag tcaggggcaa accattgaac tgaaccaact tttacgcttt
    19321 cgcctggttg aggagcatgg atatattgtc ctccgcctaa agcaatcgct acgtggtaag
    19381 ttccgccttg tgacccccag aataacaagt ctccagcttt tgcttgagaa actgaaattt
    19441 ttgttccagc tgattcttga ggaactgtcc aaccaccgat gtcacgacca gttacttgca
    19501 tgtaaacata gcgtgtgaac cctgagcagt caaagccact tggatcttta ccgccccaaa
    19561 catatggagt accaatatat ttgtaagctt ctgctacgat tgctgcacca ttcacacttc
    19621 cagaaggagc tggagcaggt gttacaggtt ttggttgttc aggtgttggt gttggcgttg
    19681 gagccggagt actacttcct gtgccgtttc ctgtatcaac actttgatct gttgatggtg
    19741 ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga ttcaggcact gttgttgatt
    19801 cttctgttgc tgaagattca ggcactgttg ttgattcttc tgttgctgaa gattcaggcg
    19861 ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgattcttct gttgaagatt
    19921 caggtgttgt tgattcttct gttgctgaag attgtgtagc tgatgatgat tcttcagtca
    19981 ttgaagattg tgtagctgat gattcttctg ttgctgagct ttgtgcagta gatgaagctt
    20041 gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc tttttcagct ttttcttgtg
    20101 cagcttgttg acgagcttgt tctgctagac gttgttgttc acgaatacga gcttgctctg
    20161 cttcagcttc tgctttttga cggttcaagt cagctttctt gtcttcagcg gttgcttgtt
    20221 cagcagctaa tgaagttttc agtacgttca aatcagcttg tttagataaa agatcgcctt
    20281 tttgagattc caaagcagct tggttttcag ctaattcttt tagtttagca tcgttttcag
    20341 cttttttgtc ttctacagct tttttatctt gtttttgttg ttccatcaag tcgttgtttg
    20401 cttttaccat cgttgtcatt gcttgaacgc gtccaatagc atctgctaat gaatcagcat
    20461 tcaatacagc atcgatgtag tttgagcttg tgttgcttac ttgtgcttcg cgtgcttgtt
    20521 tttggattgt atcttcacgt ttttcaatac gttcttgtaa atctgcaatg tcttttacta
    20581 attgtgcaga ttcttgacgc aatgtatctt gttttgctaa taggtcttgt gcttgtgtgt
    20641 tgatttcaga aacttggctt tctaaggcat cgatttgtga ttgtgcatct gcttgttgat
    20701 ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg agaatcaaaa tcgtccgccg
    20761 cggcagcgat cggtgatgct acggcagtca aggtcattga acataccatt actgctgata
    20821 ttaaactctt tttcactctt cattcctccg actggcttaa tttaataaat atctaaatat
    20881 atttaagtct ttcttaacga tataaaaatt gtaccatagc agtgtgtcaa cgatataata
    20941 gttatgttac aaaaatgttt caaaacaact tttaaaaatg acaaaactgt tcgtttcaga
    21001 aaatgatcat gaaaaagccg cgttaagtct agcatttctt aagataacaa cgattctttt
    21061 tcgctttcga cctaaaaata aaaaaagaaa ttattcatca ctgaataatt tcttaaaggg
    21121 aaaaatgaat aatacaaata taatcatgtt caacagcagt gtaggtccta aaaaccttgt
    21181 aataaaatac gtgttattta ctactgctaa tttaaaaatc aactggacca ccatcgtaaa
    21241 caattcataa cccgtgacaa agatgatcat actgaaaaat tcagtaaaga tgttgacatg
    21301 gataacgtct ttcattacat acatcaacca tacaattaaa gggagagcta ctgcatagat
    21361 accgataacg ccaatatagt aagcgtcata gatagccccg agaacaatcg tggtaatcag
    21421 taaataacgt ttttcaaacg ccatactgca acataataat gccaatatca gaaagtgggc
    21481 atttgacata taggtgcctt tcgaccactc ccctagcatt cttgttaaat gaccatctat
    21541 caacatcaaa aagaacaaaa cgattggcag atagtacttc attgtttctt tcttcaacat
    21601 ggactaatcc tccgctagcc gttggacaat cgtcacaact gacacgtcat acatttctgc
    21661 ataaggtttc acataaactt cccgatccag cccataacta tctggtttcg ttttaataac
    21721 cgtaccgatc ggcaagttag ctggagaatt ccctccaaga ccggatgtct ggacaacatc
    21781 cccttctttg atatccgtat caccagttaa ttgggtcacc actaaggcat ggagcttttc
    21841 atcatagttt ttaagcaaac caaacgcttc gccattagcc gaagataccc gtactggaaa
    21901 atgattggag ctttcattag aggatgacag taattcgatt ttagacgaag ccgtattgac
    21961 ctcgatcact cggccaatca atcctttttg cgacatgact gccatatttg cttcgatacc
    22021 gtctttagaa cccttgtcta cgatcagcat gtcctgccaa ctgtcaggcg aacgagtaat
    22081 gacatttgcc gttacttttt cgtaactagt cagcgtctga ttcaactcaa gttctttttt
    22141 caacgcttcg atttctctct cgtaattctt gttttgctgt gaaacgtcat catagctatc
    22201 gattttttct tttagccgct cattttcggc ataagttgaa aaaagattat ggacagaatc
    22261 tactccattt cgtacccacc gtactggggc agagattgtt ttatcaacca ttgcaacact
    22321 gtcattaacg atggattgga agaaattggg ctttccttca ttcgctcgct gcgcagcggt
    22381 cacacttagt atcgtcacca cgatgataac aataaccaag gtaataataa tatttttatt
    22441 aggattgaac ttcttcacaa agacacctcg aattaattag actcttgtgt tcaattttat
    22501 cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta agcttttttt tagcaaataa
    22561 tagattctgt catttttagc ctgaaagaca aattttttta tttgtttatg gatcaatctt
    22621 tgctttgaat aactgcaatc ataccatctg aaaaagaaaa ttctgcggta tctgaaagaa
    22681 attcgttact tgcataagat gtcggatatt ctacttcttt attttccaat acatacttgc
    22741 tgccttttaa tgtaagattc gagacgggag tcaagcagca ataagctaaa tacttcatat
    22801 ctggtatctg tcttactata tgctctccag gcaaataata agacaaataa ttttgacaat
    22861 ctctgataat aagctgatgg gcaaagggtt tgaagcgagg ttccatacct aaccagagat
    22921 tggctaaaag atgatccaat cttccgcccg tagctccaat caaagtgact tccgcttgcg
    22981 ggtaacgttc aaatgttttt aagataccta actgtgtatc tgtatcgtct ttttctgcag
    23041 gggaacgggt atactctttg gcttttgcaa atacttcctt ctgctcttcg ttcgacaatg
    23101 aatcaaaatc acccactgcc agatctactg ggtagcctgc ttgaagcagg tacagcgcac
    23161 cacggtctat cccgacatag gcatcgtatt tggacagcag agactgcggc cagttttgcg
    23221 gattacctcc agcagctaat aaaattttca tttagtctaa tgcctctttt aatcgttcga
    23281 tttgtccgac tggattttcg gctccataga tatatgatcc tgcaacgaag acatccgccc
    23341 ctgcatcttt acagattttt gctgtttctg gaacgattcc gccatctact tcgattgcat
    23401 aagtccaatt gttctctcta cgtagatcag cgagttccgt aattttttct actgtttctt
    23461 caataaagct ttgtccgccg aacccaggat tgacagtcat gactagtact tgatcagcca
    23521 aaggcaaaac atgtttgatc atggagactg gtgttcctgg attgataact actccagctt
    23581 taactcctaa gctttttgtc atttgaagtg cccgatgaat atgaggagtt gcttcgacgt
    23641 ggacagtaat gatatctgct ccagcttttg caaatgctgg aatgtagttt tctggatcaa
    23701 cgatcatcag atggacgtct aacggtaatt tagtaacagg acggattgcc tgaacaatat
    23761 ttggccctaa tgtgatattt gggacaaatc ggccatccat tacatctacg tggatgtaat
    23821 ctgcgcctcc tttttccact aactcaatgt ctctttgtaa attggcaaaa tctgcactca
    23881 aaatggatgg tgctatcttc attcttgttt cctcctattt tcgtttacct tctttttttg
    23941 catacattgg ttttcttttt tcgatttctt gtaagaattg caaataattg tcgtaacgtg
    24001 tctgtgcgat accacctgtt tctactctat gttttacttc gcagccaggt tctttagcat
    24061 gcatgcactc tctaaatttg caatggacag aagcttctac aaattcagga aattgtctcg
    24121 gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct aaatcctggt gtatctgcta
    24181 ctaacccgcc atagatagga atcagttcca catgacgtgt tgtatgccgt cctcttccaa
    24241 gtgcatcaga aatctctcct gttgctaaat tcagttccgg tgatatttga ttcagtagcg
    24301 tcgattttcc tgctccagac tgacccataa agaccgatag ccgttctggg aagtaacgga
    24361 tcagttctgt cgtatcaccc gtatgattgg gtacaatcac cggatagcct attttagtat
    24421 aggtttgctt gatctcttcg attttttctg ggtcgtctga caagtcggtc tttgtcaaaa
    24481 aaataatcgg ttcgatcgac tcgtattcca aggtaactag aaagcggtca agtagattat
    24541 aagaaaatgc aggctctacg atacttgtga caatgattcc aagatcaaca tttgtgactg
    24601 gtgggcgaac taattgattt tttctcggca agatttccag aagatagccg tctgtttgat
    24661 tttcactctc aaagatcacc tgatctccga caagcggcgt gattttccga ttccggaaat
    24721 ttcctcttcc tcttgtttga taggtctctc cgtttgcgta tacatagtaa aaaccgctga
    24781 ttgcttttct gatttgtcct ttgataagtg ccacctccgt ttttgtttat tttaccatac
    24841 gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa gtatatggct tgaaaattga
    24901 tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat caatgtcgca ttcccttctg
    24961 atatatttta ttcagcactt attttgaact ttctgaggtg ctagctgatg aacttgtgga
    25021 gctggaagat tcttcttttg aagagttgct tgtgctactg gagctacttg agctgctaga
    25081 actgtctgaa gagtcttttt cagaactaga agacgctgga tctggtcctt tagagtaaat
    25141 tacgttcaca accattcctt tggtaattgt cgttcctgcc cctggtactg tacgaatcac
    25201 tttatcttta tccaccgtgt ttgaatattc ggaagtctca tgcccgatat attctgcacc
    25261 gacactagct aaataacttt gtgcttcgct ttttgtatag ccggagatat cattcaattt
    25321 aggctctgtt ccttggctta cgacaaaatt gatgtaagtt tcttctgcta caacttcacc
    25381 cgaggctgga tcctgactga tgatcgaacc tgatggtaca gtgtcgctat agtcataagt
    25441 agcactaata ttattgatat tgaatccttg tgtaacaagc caattacgaa cagtttcgta
    25501 gttttctcct acatgactac tcaaagtcac tgtttctggt cctttgctga ctgtgaaagt
    25561 aatcgtgtct gttttaggat cgacttctgt atcttctgat ggagattgcg aaatgatcat
    25621 tccgctttct acctctgaac tgaattcctc ttctttttga atcaagtcag cagaaaaccc
    25681 taattctttc aaacttgctt cggcatcttc ataactttta ccactgtaat catctaattt
    25741 gatttttttc gtgccgctac taatatacag agtgacttcg cgtttttgct tgactgtcgt
    25801 tcccgctgcc gggtctgtct tcactacatt accttcttcg attttgtcat ctgcaatttc
    25861 ttctgttttt gttgctactt ctaatttcgc ttttgcgagt gtttctctag cagaagcttc
    25921 agaaagatca gaaacatcag gaacaacaac ttctcctcgc ccgccagaag caaagaatac
    25981 agctgttccg attcctaaaa cagcaagaat agctaatagg atcagccacc attttttctg
    26041 tcctttcttt ttaggatcag gcagaacttc ttcctcctct ggtaaggttt cgttttctgg
    26101 cttgtctttt ggcagaggca tagatttgaa tgaatcaggc attggtgcat cttccgtaag
    26161 gggtgtgata acttttgttt cattatccat cacatgaggc tgccattttt cttcgtttgc
    26221 acgagctggt gataatactg tagaaagatc atcagacatt tcctcggctg acttataacg
    26281 gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt tccagtgctt gtggcacatt
    26341 tggatcaagt gcttttatag atggaagatc atcttgaaaa tgttttaaag caattgttac
    26401 agcagattca ccatcaaacg gtacgctacc cgtcaacatc tcataaagga tgatccctaa
    26461 agcataaata tctgactgct tcgttgccat acttcctctg gcctgctcag gggacagata
    26521 atgaacagat cctagcatcg tattcgtctg cgtgatcgat gtttcagata aagcgatagc
    26581 aatcccgaaa tccgtgattt ttacaacacc ttcattatcg atcagtacgt tttggggttt
    26641 caaatctcgg tggatgattt gatggttatg agctaaagaa atagcagaaa ggatctgctg
    26701 cataatattt actgctgttt cataaggaac aggataatgt gtttggatat atcgtttcaa
    26761 gtccattcct ttgacgtatt ccatcaccag atactgcatg ttgtcttctt caccaacatc
    26821 gtaaacacta acaatgtttg gatgaacgag ttctgttgct gctaaagctt ctctctgaaa
    26881 tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt aagactttaa ctgctacatc
    26941 acgatccaag attaaatcgt gcgccaaaaa tacatttgcc ataccaccgc taccgatatt
    27001 tccagtgatt tgataacggc catttaattt tttgcccaac tcgatcatac actgtcctcc
    27061 ttatattcga taagaagaac ggtgatatta tccgctccgc ctgcttcatt ggcacgatca
    27121 atcagctcag ttactttatg agacaatggg ccttcttgat tgatgatcgt tcctatttct
    27181 tcttcagaca gcatgttcgt caagccatct gaacataaca gcagtcgatc ttttagttgc
    27241 caaagatgag tagaaacatc tacttcgact gttccaggca tgccgacaga acgtgttaaa
    27301 atatttttcc ttggatggtt cactgccatt tcttcgctga tttctcctga tttgactagt
    27361 tcattgacta aagaatgatc ctccgtcaat tgcatgattt tatcttcacg aatcaaataa
    27421 gctctgctat ccccaacatt ggcaatcgtg aaccagtcat ctgacagagc ggccgctacg
    27481 attgttgttc ccataccgtt atattccggc tgttcttgtc cacgctgata aatcagcata
    27541 ttttcttctt gaatcgattg aatgaaccat tgggcgattt tttcatcgtc aacagcttct
    27601 tgtttttccc aagcttcgcc tagattcgtt accgccatct gactggcaat gtctccggca
    27661 cgatgaccac ccatcccgtc tgctaataca gcaagcttga gacctgaacg gctggtaaat
    27721 acactggcat agtcttgatt tgtgttacgt cgtcttccta catctgattg atattcaatc
    27781 tgcatttttt cacctcatta ctttttgcgc aaacaacaaa tgaaaaatcc atctgtgtaa
    27841 aactgatgcg gatacaaagt caacattttc tcttcgattg atgattgtag cataggattg
    27901 gtcaaaacat caatcctctc aaaatcaggg tgtctttcca agaaagcaga aacaacgtct
    27961 tggttttctt cttttaagat cgtacaagtg ctataagtca gaataccgga agactttaaa
    28021 gttggtgcgc agctttctaa aatttccaat tggatttttg gtaaattcgc taattcatcc
    28081 ggctgttttt tataacgaat atctggtttt cgccgaagca gcccaagacc tgaacaaggc
    28141 gcgtctacta aaatccgatc aaactgctct gcttcgaact gtttactgac ttctctagca
    28201 tccatctttt gagcgtagac agcagaagag acacctagac gtgctgcatt atcttcaatg
    28261 agtttgactt tgtgttcgtg tacatctaaa gcgaccactt ctccaccgca tgacggatct
    28321 aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg cacaggcatc taagacatga
    28381 tgttcaggct cgatttgcat agacggcgct acaagcatcg aactttcatc ttggatcgtc
    28441 atgacgccat cgtggaacaa tgagctagaa gctaaatgcc cttttttcgc tactataccg
    28501 tatggactga cttggctttt ttcagctgtg atctcttctt cttgcaaaac agtgatggcc
    28561 tgatctctgg aaagtcggcg tgtatctact cgggcactag catggctagg ctgatatagc
    28621 gataaaccga gcttttcagt ttcttcgtat ccgatttgat ccaccagtcg tttcgtcatc
    28681 catagaggca ggctgatttt gacagaaagt ctttcgatag gatcagcaat gccttgcaga
    28741 tctggtgctc cttgacgctg ataattcctc aagacaccat ttacaaattt ccctgtccca
    28801 ggatttcctt ttacttttgc gatttcgacc gcctcgttta aaatagcatg tgcagggact
    28861 ttatctaaaa attctaattg g
//
LOCUS       DAYLZO010000021        27497 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.021, whole genome shotgun sequence.
ACCESSION   DAYLZO010000021 DAYLZO010000000
VERSION     DAYLZO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.021"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(716..3865)
                     /locus_tag="ACGVJG_000868"
     CDS             complement(716..3865)
                     /locus_tag="ACGVJG_000868"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFJ6556844.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3879..5123)
                     /locus_tag="ACGVJG_000869"
     CDS             complement(3879..5123)
                     /locus_tag="ACGVJG_000869"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFJ6556845.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(5116..6708)
                     /locus_tag="ACGVJG_000870"
     CDS             complement(5116..6708)
                     /locus_tag="ACGVJG_000870"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFJ6556846.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(7218..7943)
                     /locus_tag="ACGVJG_000871"
     CDS             complement(7218..7943)
                     /locus_tag="ACGVJG_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556847.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(8097..8423)
                     /locus_tag="ACGVJG_000872"
     CDS             complement(8097..8423)
                     /locus_tag="ACGVJG_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556848.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8460..8825)
                     /locus_tag="ACGVJG_000873"
     CDS             complement(8460..8825)
                     /locus_tag="ACGVJG_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFJ6556849.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8862..9077)
                     /locus_tag="ACGVJG_000874"
     CDS             complement(8862..9077)
                     /locus_tag="ACGVJG_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6556850.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            9206..9541
                     /locus_tag="ACGVJG_000875"
     CDS             9206..9541
                     /locus_tag="ACGVJG_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6556851.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9617..11614)
                     /locus_tag="ACGVJG_000876"
     CDS             complement(9617..11614)
                     /locus_tag="ACGVJG_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556852.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(11712..11987)
                     /locus_tag="ACGVJG_000877"
     CDS             complement(11712..11987)
                     /locus_tag="ACGVJG_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556853.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            12219..12953
                     /locus_tag="ACGVJG_000878"
     CDS             12219..12953
                     /locus_tag="ACGVJG_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6556854.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12992..14134
                     /locus_tag="ACGVJG_000879"
     CDS             12992..14134
                     /locus_tag="ACGVJG_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6556855.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(14762..15247)
                     /locus_tag="ACGVJG_000880"
     CDS             complement(14762..15247)
                     /locus_tag="ACGVJG_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFJ6556856.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(15249..16685)
                     /locus_tag="ACGVJG_000881"
     CDS             complement(15249..16685)
                     /locus_tag="ACGVJG_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFJ6556857.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(16718..17569)
                     /locus_tag="ACGVJG_000882"
     CDS             complement(16718..17569)
                     /locus_tag="ACGVJG_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFJ6556858.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(17725..17970)
                     /locus_tag="ACGVJG_000883"
     CDS             complement(17725..17970)
                     /locus_tag="ACGVJG_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6556859.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            18075..18848
                     /locus_tag="ACGVJG_000884"
     CDS             18075..18848
                     /locus_tag="ACGVJG_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556860.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(18901..19215)
                     /locus_tag="ACGVJG_000885"
     CDS             complement(18901..19215)
                     /locus_tag="ACGVJG_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556861.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(20734..20952)
                     /locus_tag="ACGVJG_000886"
     CDS             complement(20734..20952)
                     /locus_tag="ACGVJG_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556862.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(20967..23594)
                     /locus_tag="ACGVJG_000887"
     CDS             complement(20967..23594)
                     /locus_tag="ACGVJG_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFJ6556863.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            complement(23581..23799)
                     /locus_tag="ACGVJG_000888"
     CDS             complement(23581..23799)
                     /locus_tag="ACGVJG_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556864.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            complement(23796..24671)
                     /locus_tag="ACGVJG_000889"
     CDS             complement(23796..24671)
                     /locus_tag="ACGVJG_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556865.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            complement(24936..25235)
                     /locus_tag="ACGVJG_000890"
     CDS             complement(24936..25235)
                     /locus_tag="ACGVJG_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556866.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            25437..26234
                     /locus_tag="ACGVJG_000891"
     CDS             25437..26234
                     /locus_tag="ACGVJG_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6556867.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            26285..27427
                     /locus_tag="ACGVJG_000892"
     CDS             26285..27427
                     /locus_tag="ACGVJG_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6556868.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYLQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         8667 a         5154 c         3887 g         9789 t
ORIGIN      
        1 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
       61 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
      121 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
      181 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatatacagt
      241 cccataggta aagtgccaca ggactgtata tttttttatt tgagattacc tgaaatgatt
      301 ccatttactt tttatctcat tttttcctac tagttagtat caaatactct cctattttct
      361 caataaacta ttttgggtta catggtatct gaaacagcta aaaccttgtc attacagcaa
      421 aaaatcactt tatctccgtt tcatttcaga tacatttcac ttaagcggat tccaaaaaaa
      481 caaaagaaaa aggaacccct agttagccta tgctttttat tggttcctct caaaacacgg
      541 tcatataaca acggggtggg gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag
      601 ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa gacatctatc cctattttct
      661 acacttccac tttactacca tctttgaatc gtacaatttt cttaactctt ctcgtttacc
      721 tcaattcatt ttcaagtgga atcagctcat catctaacat tttacgccat gctcgcttca
      781 tttcttgtgg gtatttaaat ggcttcgctt ctggatggtt ggctttatat ccttcgtaat
      841 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
      901 gattagaagc atgtaattct ttctcagaaa caaaccactc attggaaaag acatgaatca
      961 aatcatcact cgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat
     1021 ctaaagtccc cacatcaaac gcatttaaaa tttgtcggta aacttctttg actccttctg
     1081 ttttattcaa tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgccg
     1141 cataagttcc tagtaattga tcaatataag tagaatcaat atcatacaac ttcgtggtat
     1201 tttcactata aaattcgatc gtggaaaaat cttctccctc tttttctggt tcttcctcta
     1261 ttaatgaacc cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat
     1321 tgttcaaggt tgaagatttc accatgtcat cattgtaatc gtcataagtc actaacgatt
     1381 cataagcacg attcaattct tggaatgctt tgacgtactc gatacggatt tcaagtggcg
     1441 tatgttcatc aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga
     1501 atcttttttt ggattcctga taacttggat aaatcaaacc cgattcttct tttgcttctg
     1561 aatacagttt tgtcgcatcc tcgacattct tctccattgt ggctggcttt cggaaagtaa
     1621 caattagtcc ttttgttttt tctggatacg tacggttcgt acgtgaaaaa gcttggatca
     1681 atcctgcata ctctagatta cgatccacaa atagggtttg aatcgtaggt gcgtcaaaac
     1741 ctgtcaatag acgatccact acgatcacta aatcaatttg tttcccaaat tgtttgaatt
     1801 ccgctctttt tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta
     1861 atgaccagga agtatcataa tactgattat attcctcaat aattttttgc atttcttctt
     1921 gttggacact tgaatctcgg ctgttttctt ctaatgaata ggtaatagcg attcgtggaa
     1981 aatcaggatc ctccatcgtt cttccttcac gaatgggctg attaggaaac tcttttgtta
     2041 accagtcagg ttctttcgtc atttctttga tcgctcgata atactttttg gccatgtcaa
     2101 tggaactcgt ggttaagatc gcagacttcg ttggacggcc atttcgaaaa tcaaattttg
     2161 tataggcatt gtctggacgg aacattttat gaatgacttt ttggatatgt tcatctgctt
     2221 caaaaacagt agggtccaaa taactttctt ttttgacagg ctccatttga tcaatcatac
     2281 gattgatctg ttccggtgaa tactctgcat atgtttctac ttcttttaat ttccgataga
     2341 ttttattctc taatgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaaa
     2401 ccgagccatc ttctaatgca ttcttgatgg tataggtatg caagacctct ccatactgat
     2461 catgggtcgt acgtgccaat tgccctttgg cttgtttccg attctcaggg aaaatcggtg
     2521 tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt
     2581 cagcacttaa cgctcgatga cactcatcta ctacaaacac gatatgttgc cccattaatt
     2641 tttggaagcg attggttcct tttttctctt cttgtttttt tgcatatttc aacgccgcat
     2701 ctagcttttg acgggttgta ataatcacaa catttgcatt ggcatctgcc aacagagttt
     2761 cacttaactc tttggcactt cctgttccca cgatcaagct attcgctttg gcgttcccgg
     2821 aagaaatccc agtattaaat tccgaagcaa atttcgtaaa ctctgtggtg gtttgattat
     2881 ctaaatcttt gcggtccact aacatgatcg tccgatcgat ccccgatttt cgtgctaaaa
     2941 gcttagtaga tacaaaacta gtcaacgttt ttcctgaacc agtcgcatgc cacacatatc
     3001 ctgattgatg tttatttgca gcaataaaca aggcttgaat cgcatgtact tgataaggat
     3061 gtaacaccat gaggatttta ttgtcttgat cttcactgac aatcgtataa tccgcaatca
     3121 agcgatgagc atccgggata tttagcactt gtttgcaaaa ctcatataag ttttctacct
     3181 ttttattatc tttcgttcgc caactaaata aaaacttagg atgcaaatcc tttggtaagg
     3241 catttgcaaa ataccgagtg gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa
     3301 agatattgtt ccgaaacata ccttcttctg catatttttt gatttggtta aacgcctgat
     3361 aaaaactatc ttttgcggtt acttgtttca gctcgatttg gacaatcggt aatccattga
     3421 tcagtaaggt gacgtcaaaa cgtcgatccc gtgcttctat gttacttcca cgttttgcaa
     3481 tttgatggac cacttcatag gtagagattc ctccaccaat gtcttgatta gaataaagaa
     3541 tgagcgagac agaacccagt tgcggatctt cccgttcaat cgtgatccga gccattccat
     3601 tttcaccttt taaccatttc gcagcatcaa agggggtttt cgttcgtagt aacaattctg
     3661 ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc attcagttct gcttgattat
     3721 ttgagatgat tttttggcgt aaattgaccc aaagatcttc ttctgatttt agatcgggac
     3781 gatacgtcca ctgattatgc ccttccccca atacacggat caactgttct tcgacttccg
     3841 cttcctcacg gtgactaatt ttcatttatc ttccctccct aaacaaacat cttttgtaag
     3901 aagccttttt tcgtttcctt cagcaaatct aacttacgtt gatgaagagc gatcgtgtcg
     3961 tcgagttgtt tgaagaaagc gccgattttt tgttgttcct ctttattcct agtaaaagga
     4021 aacttgatat tagcgaaatt ctcatagaat aagtattctc gtacgcttcc ttcagtgttg
     4081 cgcttgacct ctttgataaa tgttggtgac ttgataaact gaagtatgaa ctcattatct
     4141 aagtcctcgc tcattttcac aactacataa agagagctaa caatgaccgt cataccaaga
     4201 ttgttaaatg caattgatcc tacgtttact cttgctggat tataggcgaa ttcatttggt
     4261 tcaacgaatt tataatttgt tttttctaaa tcatccaacc tactaccatc aaactgttct
     4321 gtttgagaaa ttaaacctgc tttattactt accgaatagg cagggaagtc taaatctttc
     4381 gtgttctttt ttccagtctt cttactaaac tctttgaact tacgctgttc ccaatcttcc
     4441 gtaaatccag gaaaccgaat ttctggcact tttgctccgt ttttagggaa cattttttgt
     4501 aagaatcctt ttttcgtttc ctttaataaa tctagcttac gttgttgaag agcgatagtg
     4561 tcgtccagtt gtttgaagaa gttcccaatt ttcttttgtt cttctaatat tggaaattgt
     4621 actatccatt ttttcacttc tgacattggt acgagatttg taattgctga acctggatta
     4681 gctcctgtga gttttctttg cataaaatct gcacgaataa attgaataaa atattctgaa
     4741 tctgtgattt ctttatttat tctaagtcta atcaaagcct gattgaatac acccggtttt
     4801 atttgctttg gtactcttga aattctccca attgttccag cgccactcat gataaaatcg
     4861 cctggttcta atttaaattt gattaattca ttatattttt tttcagtaat tttatatctt
     4921 gtttcgtaat tatcataaat agcattttgt tgttcataga caacaaaatt gctattagat
     4981 acaaagaatt ctttctttaa ggcactccca aatggtcccc tccttactcc atctttttta
     5041 tcaagtaagc attcaaactt acgctgttcc caagcttccg taaatcctgg gaaacgaatt
     5101 tctggttgta tatcattact catgtttgaa cacctccagc gcaccttgta accacgcttc
     5161 attttcaggt gttgtttgca gggaagagat agcttctaaa agttcttttt ctagcacttg
     5221 ctgttcttgt cgtatcttct tgatgtcatt tccgacatga accatatcaa ttggttcttc
     5281 ttcttcaaac gtatctacat agcgtggaat atttaagtta tagtcatttt ctttgatttc
     5341 atcaaagttt gccaaatgag cgtatttctc gacatctttt ctttcagcat aggtttcgag
     5401 gattttttga atgttttctt ccgataattt attctgattt tttcctttga caaattctcg
     5461 actagcgtca atgaataata cgtctcgagt ttggcggttc tttttcaata caatcaccgt
     5521 cgttggaatc gatgtcccga agaacaagtt cgcaggcata ccgatcacag cataaatact
     5581 gccgtcttct agtaattttt gacgaatgac cccttctgca gcgccacgga ataaaactcc
     5641 gtgtggcaag acgatggcca tagtccctgt ttccttcaaa tgatagaaac catgaagaag
     5701 aaacgcaaaa tctgctttgg attttggcgc taattttcca taacgattaa accgagaatc
     5761 gtctaagaag gtcgtatctg ctgaccaatt agccgaataa ggtgggttca ttactactgc
     5821 atcaaatgtg taaggttcat ctgttggcca atccttattc aacgtatccc cattgcggag
     5881 attcatttct tcggcatcta ccccatgtaa aatcaagttc atttttgcta gattgtatgt
     5941 cgttgtattt aattcttgcc catgatattt aacgttatct gggtgagtta agtagttacg
     6001 cacatttaac atcaatgaac ctgaacccat ggttggatca aaaacactaa agaatcgtcg
     6061 ttctttttga tccaacgtca cgatttgtgc catcatatca gaaaccatat gaggcgtgta
     6121 aaattctccg gctttcttcc ctgcttctga agcaaattga ctgatcagaa attcataagc
     6181 atccccgatc acgtccccat cgtgacctaa aacttccacg tcattcaatt tcttaatgac
     6241 ttcggtaatc gtcacatttc gttgttgctc atctgttcct agtttttttg attggagatc
     6301 cacatcatca aataacccat taaattgatt gtaggtagat gccaattgca caaaggcttt
     6361 gtttagatca ttcaattgga acgttgcttg ttttgcttga tccgctaaga cgttgaaaag
     6421 atactctggc gaaatcgcat accccaaaat atcaacgatt gttgcaatca gatcttcttt
     6481 tgattcttca tctgaaagta attcgcgata caacatcgtt tgtttgctta ccgtatcata
     6541 ttcctctaag gattcatctg ctaaaagaac cacttgttcc aacaaacggt cggataaata
     6601 tttatagaaa atcagtccca ataaataatt tttatattct gacgcatcca ttttgctacg
     6661 gagattatct gccgcactaa ataatttttg atttaattct gcacccaaaa agatacactc
     6721 ctatctctta tttaaagttg ctatataccc tcttatttta gcatactccc ttttagaaaa
     6781 agcaaaaaaa aaaagaaaga aacttattct tgctaataaa ctacgcgaaa ggagtctaac
     6841 gttgtttgat ataacttaga ctgatcgtta acttattttt tatatttcta catagttatc
     6901 tttagaacca tttcagatac atatgaaaca attacatatg attttttcat aaaaaaacag
     6961 tactgtctag tagtataaaa tatacaaatt tcttaaaaat tttttcacta aaatcactac
     7021 ctgaaacagt ctaaaacact tatatatcaa cgtttaatga aactatctcc gtttcatttc
     7081 agatacaaat aaatatagtc gggctccaaa tccgaaaaaa aataccccca gtagcttatc
     7141 ctaagctact ggagatgtat aagtacggtc atataacaac ggggtagggt aaataaatga
     7201 aaaagatgga agaatcatta ttcaataact tttttaaata cttcgttgta atcagttacc
     7261 tgtccgttct taaataccac agaataatct tttttcccat tttcaagtaa ccgaagtgga
     7321 atatctttac caactttctc tgtcactgca ttactgatac ctgttagatt agctaataaa
     7381 tctgataaag attgagcatc tgtagaaatc tctgtatcag tatttatcgt gattccttta
     7441 tcttcattta caagtttcca agtaggtggt atagctgtgt atgtttctac aaatgaatta
     7501 agtttttcat taagtgactt ctcttttata tttccatcta aaaactctgt atatatggct
     7561 tgagctatac taaaacaaag ctggccttgt tctaataatt ttacatcatt agttagtaaa
     7621 gctgtgttca cgttatcaat cgcttcaaga agagtatctt tggtgtccct aacagcttta
     7681 ggtgtgcctt ctggaatttc cctacttgat acctcgtcca ttttatcttc cataaacccc
     7741 ttaaaatcat atctttctcc caaatccata gaagtttttg agaaagcttt catttctgag
     7801 tctatttctt caataatatt taacatgtct gatacatatt gtttaggctg attcgttttt
     7861 aatctatatg tggtaaatat ctcaattcct ccaattatca ataaaattgc caaacaaatc
     7921 cataataatt ttttcttttt catttttcct cctatacact gctgttataa cagtgactaa
     7981 tgtaaagatt ttcccagcaa tcatcagact attatttaca gggaatcaaa aaaactcctt
     8041 agtaagctta cacctttagg agttcctgtt tgaacggtat gcataataca tttaaatcaa
     8101 tttaacatta acacctcagt atatggtatc tctacatata cacggtcttt ttttggatag
     8161 tactcgacat ttatattgat agcacttcca ttgtctaacg tagctttagc ccggtacgtg
     8221 ttatcaccat tatcatgagt gatcgtcaca tcatcacatg tccgatttaa gttatactgt
     8281 tcttgtaaaa tctgagttac caatggtaca gatgacttct ctagtccaga tgaacccgtc
     8341 attcttgaaa aaattatacc tataatagcc aatacaacga tacctataat gacaaacttc
     8401 ttattcatat ttactttatt caacttttta tctcctatct ccaaaatgat tttctctcat
     8461 cagtaactca ttgacatccc caatagtaca ccaaagatgg ctccagctac ccctgctatc
     8521 atcaaaatcg tacccatttg ttcatctttc tctcgatata agtaacccat aataacagca
     8581 acagcaccaa ataccacagg aataaatgct aatgatacta ccatagaaat ccaaccaata
     8641 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
     8701 gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtaat
     8761 gctccgcaaa actggcaata cttactttca tttgttattt ctttaccaca ttttctacaa
     8821 tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gctaactgtt tactttatta
     8881 aatacatcag aaaacagctc atttattggt acacttaata tctcagaaag cattttcatt
     8941 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
     9001 ttcatttttt tggctaactc agtttgggaa agtccttttt cttttctgta tcggctgaga
     9061 ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat
     9121 ttcaactcat aaaatatata aatacttcaa atgtcattac aaatacaaca acttgttgta
     9181 ctctactgag aaggggggtg tattaatgaa acagaagcaa tatgcaaagg tcattagaga
     9241 aaagcgaaaa gcactcgggt ggacacaaaa agaattagct aaaaaaatct tctctacaca
     9301 acaggcagta gcaagatggg aaaattctgt aacagaacct aacctcgata gcttaacagc
     9361 tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg acatagatta
     9421 tgaggaagag tttttggctc tctatcgctc tcttagtact gaagatgcaa ttcgaacaat
     9481 tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat tgaaagattg
     9541 aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat aagattaaga
     9601 gctatggata acctgtctag tttaatataa ttgggtctct tctattcctt actctccgtc
     9661 catcatctct tgttacactt gtaaattgat aaataggaac taattcaact cctgataata
     9721 tcaattcttt tattacttca tctatccctg cccctttcgc tagatatact aaaaaactac
     9781 tgcttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca agagtgaatc
     9841 cttgtttaac aaagtagcct ctgttttcta ttaaagcatt tttcacgctg ttatccctga
     9901 catttcgtag gcgttcacaa acagtgttaa taagttcttt ttctgtcatt agatatgtcc
     9961 cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat tgtttttcct
    10021 tgctaagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt tcgttaaaac
    10081 atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaaatttt tccagaagtg
    10141 gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg tctaatcgat
    10201 agacattgct tctcttatgt ttaaagtttc ctaacgcttt ctcttccttt aacctattta
    10261 ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc aatcctataa
    10321 aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta ccatagtaag
    10381 aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt tccttactat
    10441 catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt tcctctagat
    10501 aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca aaaagtactt
    10561 ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc tctaatagcc
    10621 aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact ccaataaaat
    10681 tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca gttttactgg
    10741 taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag tttttacttg
    10801 acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca ttaaaagaca
    10861 tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct ttgtcgtcat
    10921 tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc cctgaatcac
    10981 tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta atatctaaca
    11041 tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa aacattctat
    11101 tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat tcgtaaacag
    11161 caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc ttgtattttt
    11221 ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta tgaactaaat
    11281 tgatagataa aaccttttct atgcttaagt atctactatc atctttactc aaatctacta
    11341 ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact ctggtttcca
    11401 caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat gattgtgatg
    11461 aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct aaacacctat
    11521 ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt tctttatcta
    11581 cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta taaaattctg
    11641 ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat gttttagtct
    11701 tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga ttttttaaat
    11761 ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata ttttctaatc
    11821 gttcctactg atacacctag atattcactt gcctgctgat agtccatata ctctagagtg
    11881 actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc tttcacttgt
    11941 tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt tccccctcct
    12001 ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc tcaaaataaa
    12061 agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca tcgacataca
    12121 cataatacct cgataaaaaa ggggtgtcaa tagttttgta aaatttattt acaacacccc
    12181 ttttttgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt ccaccaataa
    12241 attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga aagagtaaaa
    12301 aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt gacaaaaaga
    12361 ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta tcccgaattg
    12421 ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa gaatatattt
    12481 tgggaattat tctttataga gatagcattg tactagatgg tataacattt ccagataaaa
    12541 acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta gatacatggc
    12601 tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac aagacttgta
    12661 ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa gaattattca
    12721 tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc agtatagaag
    12781 aactactaga aagaatggta gatgaatggc tacctattca attgaaagac atgtcttatc
    12841 ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa acgatttcat
    12901 caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc tagtggtaag
    12961 tactccttgt aaactaagag gagatgattt catggcaaaa atatcaaagt acaaaaagaa
    13021 aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac gaactggtga
    13081 aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa aagattatga
    13141 cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata tgacatttga
    13201 agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt caacaatcgc
    13261 aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc ttaaactatc
    13321 gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag
    13381 ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg caatgcaata
    13441 tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt atgagaaaaa
    13501 tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta aagattttgg
    13561 taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga gaaagagtga
    13621 agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac atgtcaataa
    13681 aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa caagagcttc
    13741 tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact ggaaattaca
    13801 gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc aacatgtttt
    13861 tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt taagatatat
    13921 tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata cacatgctag
    13981 tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa
    14041 tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta agaaaacagg
    14101 tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat ctggtcattt
    14161 ctagtataca ggcaaaacaa aaaagctaga acccttatat atcaagggtt ctagctccta
    14221 taacaggttt acccaacact gccttccaat gactcttttg ggaatcagag gaaagaatca
    14281 ggctttatag agatatttca tctaacaaat tactttagac tgacagtata tatcagcagg
    14341 tactgtcatc gggaacgtag ccaatatcgt atccaaaaca gcaggtagag aagctatttg
    14401 aggattcaag ttattctatt tcaaccatga agttaagttc attaaaaata aagatagttg
    14461 gtatttcaat aatttttgta tagagttaat cactttattg atgctaattc atcttgattg
    14521 gtattataat cgtattcaag ttgcgtggcg tgcttttgct agaaaatata taactgttct
    14581 ttataaagaa atataaggat taaacatttt ttatcataga gttttactta tctatagaat
    14641 tatgtctgtt cctaattcag tttgatgaat taactgataa aaaaatatct actatttaaa
    14701 gatacaggga taagtaagta aaaaaataac atgtcattag atagtagaca gttccaactc
    14761 tttatttacc ttttttgtat gctgcactgt caatccaagt tcctaaattg ttgtatccat
    14821 catcacttat ataatcatat tggccatcaa taatttcgtc aaaccacaaa tattctccat
    14881 ctggtttttg cccaattatt ttaattactg attgactagt agttgtcatc tggttaaaat
    14941 cttttatttt acctatatta acggccaaaa tagcatttcc tcgcttataa ctctcctgga
    15001 tttcatattt aacatattct cgatcaagag tttctttacc tatcagcacc acagtcactg
    15061 atgttccaat taattgatta tctatccagt tttttatagc aatatctcca ttacgtttaa
    15121 ctttctcaaa ttcagcctta tctacaaaac cagcttcctt tgttcctcgc aaagtccagc
    15181 tgtttctaac taccattgct cttgttacat ctgcataatg gaaactaaaa aaaacccttc
    15241 tcaccatatt aatcctcctg aatactattt attaaactga caatttgttc aactttttta
    15301 tctacttctc cttcagtatc taatttttct agcgcttgaa atttactttt taaggaatca
    15361 tcaaaataat cactgaaatt ctcttgaatc ttatcaaaca aaaccttact tgcacctttg
    15421 gtaaatccaa tcggaactac tatattttca ttttgaacag ataattcaaa ttcttcaatc
    15481 attccatctg ctgtaactat ttcacctgat tcaagtttgt ttccaaaaac aaaaatacta
    15541 atcccagact ctgcaataat gttttctcga tatcttctcc actcatgagg catatcaagg
    15601 ggaagtggtc ttaaagaaag tacgtcttgt atgttatttt ttcttaaatt tcttctctgc
    15661 tttagaactc ctgaaacaat actattacca attccaagtc cgtaacctga tactattcta
    15721 taaccggatt cagaaagcct atatgctaat ttctcgacaa attttaatcc tttcgattca
    15781 gatgtaaatg gagtataatc aacagcactt cccgaaataa aaatagtttt tctcaaatac
    15841 ttttttctaa tacttttcaa aatatcagta atctcagaat aatcatctac ttcaacaacg
    15901 tttatcgaaa atctggacaa atcattgatt tgtagtcttc tttttgtcag ttcataatta
    15961 tatctctctt gatcaaaaaa atcctcagaa tcgacaaaat tttcctttaa tatttttctc
    16021 attatacaat aatgaggtct ttggttttct ctcaaaaccc atcttatatc tgataaaatt
    16081 ctttctaaat tagggtctgt aaaactaaat cctaaaaaga gaaaagtttt tgttaacaaa
    16141 tctcctttta aagtttcttt aaataacgcg tacgaatcat catcatattt ttcataatca
    16201 tctcttgtta ttactgtcga actgacatca tctatatcac catgcatttt ataaacaatt
    16261 gtatccacat ttggattagt tgttgctaac tgagtataat ttttcttaac ctgagggttt
    16321 ttataagctt ctcggattga tttttctatt agatgatcat aattagttgt ccaataagtt
    16381 tcaataggta aacttgctag tattcgatga ttttcagata tttctaattc ttcaccaaaa
    16441 ttatccataa gtatctgatt caaaattcct cttgatccac cattttcatt tttataatat
    16501 tgagccagag taactaagtc tgtttctttt ttagaatcca acccgatttc tgttgcagga
    16561 tcattaagta gttccggcca acttacatat ccttgtgaca tcgataatcc tgcacctgca
    16621 aatattgctg catttccttt ttcaatagct tcagagaacc tctttattaa aactttctta
    16681 tccattctac ccggttcctt tcgattcagt aaattgatta gagattagtg aaaatggtgt
    16741 ttttatataa aatatctaat gtattacttc ctttagtgca ttccacagca tctaaagccc
    16801 attttttcaa atcagctttt cgtaaataag ttaattcact taaatttaaa gaatatttta
    16861 tttgcagatt ttcttgcgaa gaatagtgct ttataatgct tctaacatcc tcggtgctca
    16921 tttgaggatt actttccaat cgtggattct ttttttccac tattgaagct gtcgtcagtt
    16981 cgctatatag ccatggcgaa tacgtaaatt tctgttcatt agtgatttca ttgctcaaac
    17041 taattgaatt aggtgtatta agaaaaaaca gacattctgt actatcaatc atattattta
    17101 aagctgtaga aagcatcata tgaacatgac tagttgttac atttctttta tcataatcat
    17161 atgtttttgt ttgttcctta tagcaatatt tttggtctat tttttttaat aaatcagaac
    17221 tgtatcccca aaccgaagaa tcaataaatg cagtaagacc aaaattttca aatagccatg
    17281 atgcaaaacg tatagctaat ttttcatcag catgagagtg agatataaaa atatctgctt
    17341 ttatagttgg aaaccaatct ttaataattt cgtctgcatc tagaattttg ctgtccttag
    17401 aataataatc aactaaatca ttttcacttt caaatagatt ttctattctc ttgttaatag
    17461 atttttggtt gtgatctatt tgatttacta catgtgtata tgattgcgag cttgaggaga
    17521 atgcatttaa gtcagcattt gtaaaatcca attcatatgc cttatacaag gatatccctc
    17581 cattcttgta ttttgatcca caatttatta ggcaacataa cagtataccc tatcgttaca
    17641 tatcccattc attattatac cagtatttct tcgtgacaca taagggtgta acataataat
    17701 ttcaaacgaa atgactacaa gtagtcattg aagcaaatct tcataagaca cctgtagaat
    17761 atctttgatg gctttcaatt gatccaactt aatgtgttgg cggccacctt caatcttaac
    17821 caacgtttcc ctagttatag caattttacg aagctgtagg tctctaatca gctcggtttg
    17881 accaattcct ttttctaatc ggactcgtct aatattgcta ccgattacat tttctttatc
    17941 aataattttt tcctctttca agaatagcac accttttgga ctaaaattag tcctttttct
    18001 tattaatctt aaagattcgg tatgctacaa tgggactagt attagtccat attcaatttt
    18061 aaggaggaat ttttttgaag aaatttgcaa tcattctaac ttgcgcttta ctcatcggaa
    18121 ctataagcgg gtgtaccact tctgacacaa gttctacaag tacggaaact agtacaacaa
    18181 cctcagaaaa gaattaccca atacattttt ttccggttcc agatggattt gaagcttatt
    18241 atcatgataa aaaactagat gttcctttat cagaaactga atctactgaa aagattttgc
    18301 caattgaaat tgaaagaaat caagacgata taaaactaaa agctgatctt cccttactga
    18361 acagcacaca aattgataga catgaaaata ttaaagttaa tattgaaaaa ggagcgttag
    18421 cacaacttta tactgaaaaa gttgaacaat tttttgaggc tggtgccaat aaagactttt
    18481 caaaaataag cgattattcg gataactttt ttaatgagac ccaagatgaa atcacgaact
    18541 caacttttat ggctgaagat atgtacaata tgaagttgaa aaaaataaag tcgtatgatt
    18601 atccattaaa tctaacatta aataaaggga tactctcttt tgtattagca ggttacgttg
    18661 actatgaata tacagcagat tatttggaga cgccccttca acacgataat aacgatatta
    18721 attttagatt tatctacaat tcaaaaagta aaacatggtt agttgatcaa ttgagcacta
    18781 taaatacggg atttcatgaa ttacctaagc ctgacaatga aactgctttg gtcattaaaa
    18841 cattctaaaa aagaaacgtt gaaggaataa ttattctttc aacgtttctt ttattgtttt
    18901 tcagtttacg gtcttacctg agcgaataat ctgatttcta aatgattgga aaacttcttg
    18961 tggttgataa taatctgtat ctatttcaaa tgtttctgag aataattcat tgaatttttc
    19021 tactgaaatg atgattttac cattctcctc tcgactgttt tctattattt ctgcctctaa
    19081 ttcaaataaa tctagtgacg aactaaaatc ataaatcctg tcatattgaa caaaaaaatc
    19141 aagaaaaagt tgctcaaact cttccttatt tatcactagt gtttcaagcg gaacaacttt
    19201 attttcatca gccattcttc caatttcctc actttcattt tcaattgtcc attgttgcgg
    19261 acgtatcaga ttttctctat catttttcag tgtatagatc acttgtaata cacaacaatg
    19321 ctttgtaaac ttattttttt agttagtcga atctttttaa atgtgcgttt cataccatgc
    19381 gcttgatata ttttctccta ttttttaaat cgtttatcac aacttttatc gttttttctt
    19441 ctgaaaaaaa tcttgtttac atcgttacat agtttccaaa aagatacatt actagtagaa
    19501 acaacaagat taattttact tcccactgaa atgggaagta tattttaaat attttttctc
    19561 cttcaactta cactataaag tatcttaatc acacgatatg ctagccacta aattcatcat
    19621 atttgattcc ttttttaaga taatgtagca tctttttatc caataacgca attgttttta
    19681 aggataaatt tcttaagatg gtacatttca aaatggcatt tgacatatga aacaaaaaag
    19741 aaagaaaaga gcataaggga aaactgatag caccttccta caggaaggtg gctaagttgg
    19801 ctgtgccaac tggttttctt tcaaaatcat ttcataattt ttgatatcat aaaaaaatcc
    19861 attttggacc tattttcggt cataatttag tacctacttt tggtcatagt ttcgtccgta
    19921 gttttggtca tagtgactaa ggcttttctg ttcacaaaat tggtttaaat ttgctttctt
    19981 taacacttca tttatatcat agcaattata atgtttacac aacatcattc ctaattttaa
    20041 cggttgttcc tctttatatt ttttaagttc tacatttttt tgcgtttaat tacttttctc
    20101 ttttctttat attccgctcc aactttctct ttaatagtga taatagaatt acatattttt
    20161 gttaaacttc cttaaaaaaa ttggatctcg attactcaaa tttgcttgca ggcttttcta
    20221 attgctttgt tttttcttcc taattatttg attttatttc cattttcttc tagtgaaaat
    20281 ttattatttt taataatcgt acgcgtagaa ctgatttcat tttttgtatc tgtttgatta
    20341 tctaacgttt gaaattcaaa gttttgattg gctttatcaa tatcttttta tactacttta
    20401 tttgcaagat atattttctt gcttaatttg gtcatagttt cattcgtcac ttcggtgata
    20461 tttttttaat ttgcaaataa aaagagttaa accgatatac attaggttca actcttttgc
    20521 aggtttcatg ggtgtactat cacttttaat ctgatagatg taaacctctc tttatctttt
    20581 tgttacttca attttcaaca atagctcctt tataggttgt caatattgct ctacgtattc
    20641 tggcttttta acccattatt tctctaaagt ggaagagaat aatcgcttaa tccccactaa
    20701 ttccttgtcg taaattcatg tcaaatacaa tttttaaaag ccatttaatt catggattat
    20761 ctcttcactt ttttctagat aacaatattt caagttgttt tttatctcat gtttcatctt
    20821 taattcttct tgaatcatca gttcaatata attagtgacc gtgatttttt catttgttgc
    20881 gttctttact gattcacata attcgtcaag ttctaagaaa tcatgcgtac taatctttag
    20941 aattaatttc atgacaattc ccccttttat ttttttaata gattatcgaa aagcggatta
    21001 ggatcattaa gtgtatctct gaataactcg ttgtcttcat aggtaaatct tcgtcttggt
    21061 tttgatccct tatcattttc acggatttca ctatcaataa tcactgtttc atgatttgaa
    21121 tcaaattttt tcgctgactt tctgtcttca acgatattga ttttttgtgt tttttctttc
    21181 atttctccac tatctttttg aacgttctcc cgaacatcta ctgttttgaa tccttgacta
    21241 cttgtagaac tttgtacatt ctctcttaca tccattgatt taaacccttg actacttgta
    21301 gaattttgta cattctctct tacatccatt gatttaaacc cttgactact tgtagaattt
    21361 tgtacatttt ctcgaacatc aattgtctta gtaccttgac catttgaaga gctttgtaaa
    21421 ttctctctta catcaacatt ccgtccgaca tgaccattca atgaaccttg aatattttct
    21481 cggacatcta ctgatcggcc accttgacta cttgtagaat tttgtagatt ttctttgacc
    21541 aatttttcta atccggtatt tgagttttta gcttcttgga ttctttcttt ggtcaacgct
    21601 tctttttcgt ccgtattagc tcctgtacgc ccttctacac gtttccttaa catttcctca
    21661 cgattcattg cttcaggcgt gtatttagct tcttgtcgct tctgattaat taattcttca
    21721 gcagacttag cttgaccgtg catagcattt ttacgttctt caatatcttg ttgaactttt
    21781 tgctgaattg cacttacagg attattattc gattcagcat tatttttcat tgcttcacgc
    21841 atacgttcag aactagacat aattctatcg gcattttcaa ccttagcacc atcttttcta
    21901 tccgaaagac gtgttggcat ggaatcagca tattttccag tttcatcttc tttattttta
    21961 gctaacatag gtttgctatt tttattaata gcggacactg taccatcatc taaaccttct
    22021 ttgaacttat tcttggtatc attagccata tcacgcatac tcttaaatgg ttttattgct
    22081 ctttcactag ccattgtagc tccatctgaa gctaaacctg ataatccacg ttcatgggca
    22141 tatcccaaag ctcttccagt tttgccagct ttttttgata gagtttcgcc gaaattcttt
    22201 tctggttttc gatcttctcc ttctccactg tctgatccat ttccatttcc gcttccttta
    22261 gcacgattaa atccagttga tccttttcta aacagcattg cgcccatacc aaaagcagaa
    22321 gcaagttgcc catagccttc tttgagtcca atatcaacac caaagtatct catgactgtt
    22381 ccactgcctt ttatcagcac aaacacagca cagatataag caattgtttt tataaaaata
    22441 tttgtggaga taccttgtcc agcatttaaa aaattgataa acatagcaaa catagatagt
    22501 ccaaaccctt gaaatccaac tgttaagtaa cattgtaaaa tatcggataa aacaactttt
    22561 gaacgttgtc ctgtttcaat atctgtcgca aaaacaagaa cacctaacac acgtttaaat
    22621 gcaagttcta atattgctgt aatgatcaca aacgcgctga acacataggc gattgccaac
    22681 gctgtcaacc caatcgaaat cccccaaaga tttgcttgat atcgataata acctgatttc
    22741 aagctatcag aaaatgttga taagaagtta tcagaaaact ttgtggcaac aaattcatta
    22801 ttagaatttt caactaattc atagcgaaga ttatccgcag ctggattttc tttttccaat
    22861 ttatcaatca ctttatccgt caacacttgt gttaaatctg tctgttgaaa attttttcgt
    22921 gttaacttat ttcgatcctc cgttttatca atgcttgagt attgatctgt tttattaata
    22981 tatgccagat ctgttactcc ttgttttatc aatgaccaag cgactccgtc atctgaattt
    23041 gtaatcgtag tagcatcatt gtaaaatatt ttggcaaatt gaataccgct agaaatcatt
    23101 gttggcatta aaagaattaa cgctacagac atcactatat tcattccaac agatcttaaa
    23161 tctattgttc cttttccagt aatcatcttg tagccaacaa acatcaatga aaggatcatt
    23221 aatccaacga caattgtatt tatgactgat tgataaacat tatttagccc tgtactttca
    23281 atgaaattga acaaatccaa cacatctgga atcatatttt gaatcgaatc agtcacagta
    23341 aacatgcctt ttaccacagc ccatccaagc caaatcccga actgactaag gaaatctcca
    23401 ttgtattttt ttaggtaagg tctataaaga gaattattca aaaactctgt ttgacttttt
    23461 gtgtcatcaa accaattgaa aatgctagct tcaacaatat tacttgtaaa aacaacgcta
    23521 atcattaaaa caagtacact gagtgctaac cagtttattt ttctactaac gattttttct
    23581 ttaattgctt tcatagtttt ctccttcagc ccattttctt aggctctcag agagctgctg
    23641 agccattttt tgattaatta tgatatgagt tacacaattg tcattttcag ctcttaaatc
    23701 gagaactact gtgttgaagc agatactctc gattaattcg agagtatctg tattttgatc
    23761 aagacaccac atttgatcta ttaagcgatt tttaatcatg attcttctcc tttcaaccaa
    23821 ttatgcatag taatttcagg taactctttg atttcgaaag cttttttgaa ctgatctata
    23881 ggctctacat tttctaaaaa ttgactttta tatcttttct gaagtccatt caaaaaatat
    23941 gttacataat cgtataaccc ttcttcagtt tttctttgaa aaaagcatgc ttcatgttgt
    24001 tttataatac actcagcaat aagatactga gtattttcag ataaatattc aatgattttt
    24061 ctgatatctt ctgtatctac attagcgttt ctcaaatagt gatacacttg aaataatttt
    24121 cgaccatctt cgttatttgc gtcataaaga acactaatct tactgtaaat cctctttata
    24181 ttaatatcct cttcttccat aacgagagta gtctttgata tgttctgtga agtagtcttt
    24241 gtatacgtat atacaaattt gtacatacct ctaccttttt ttaaagagac atatctcttt
    24301 ttttcgatac ccctctgacg aattttcggc atatactcct ttttttcgca atccaaagta
    24361 ggaaaagtaa tttctatcaa tttatgtaca tttaaaacaa tgtacataac atttgataat
    24421 gtaactgctc ttttctcata ataaatattt ctaaaaaccc ttttgataat cccctttttc
    24481 tctaagtaca caattgcatc ttttatagct cgttctgata atcctgtttt tttgcttaat
    24541 tgcttatatg atttttgcaa aatatcttca agaaactttt tttgataacc aagtagctga
    24601 ccatcatcgt taaaaatttt tttaggtcta taccaatagg taatttctgc taagataact
    24661 attgcattca aatagggctt ctcaacacct tttttcgttt tttttcttaa ttgatcgtac
    24721 catgtgtgtg gattacattt ccgtcaaaat ttatttctct aagtttgtca actacaacat
    24781 ttccagaaga aaacaccttt tcaccttctt ttttgatata atagctataa ataaagtttt
    24841 ctcagcactt tattattgag tgttccccca gtctgcaaac ttttgggaac actttttttc
    24901 tttttttaaa gcattttttt ataatttcta cttcatcatc ttccaatatt ttcccctctt
    24961 ttcattcgag caaacataaa ctcattaact gcctttttcg aataaaattt tcttgcccct
    25021 aattcaacta cgggcaatcc ctcatttctc acagcatttt ttaaaaagtt ttcactcaca
    25081 tttaagtatt ccgccatttc tttttgattg agatattccc ttgtcatccc cgcattttct
    25141 tgagcctttt caatagcttt tgtagcaatt tgataaaact gctttagaaa catttcctcc
    25201 tgttcttctg aaatcattaa ttttattgct tccacaaaaa cctctccttt ttaattaatt
    25261 attctataac tcctctacct ttataagtac aaataaatac aaattgcgta aaaaaatgta
    25321 tttatttgta tttataagaa taaagtatca tactccagat aaaaagtaaa tacttttaaa
    25381 tacttttatt ttataaatat gatatactat ctttaaaaat aaaaaggaga aaaaagatga
    25441 agccaaatcc ttcagaagca ggaaaacgaa tcaaacaatt acgcctttca tgtggtttta
    25501 ctatggaaga acttggaagg aaaatagata attcaccacg tgcgaccata tccaactggg
    25561 aacgtgggac caatttacca aatccacaaa agctgaaatt attatcaact atcactaatt
    25621 caacaattga ctggattaaa tggggaactt tagaagaata tataacttca taccttatcg
    25681 atatagggta tgagctatat attaaggact tcccagaaat acctcacaaa gtctttaaag
    25741 acattcaaga aagatatagt gatacgtttt cgctagataa agactatgag ttattgaatc
    25801 ctataataaa aaatatattt acaaaatatt actcaaaaga ttttgaaatt tatctaaatg
    25861 agatacttgc taataatatc aatcctctga ttgaagagta cgctaaccaa agacactcta
    25921 tctctaaaga gctatttttc aaaagagcca acagtaaact tcaagacaag ataagaaatg
    25981 gtgatttcaa atacggtgaa aaagaaagaa tgattgattc tttaaaacag ctattagacg
    26041 aaatggactt agcctataaa gatatttcaa agtattcatc tgtcgaggat ttcttttata
    26101 aaattactaa aaaccaattt gaaacagaaa aatttatttc agatcttagt aaaaaataca
    26161 atttcccata tgagaaaaat agtataatcg caaaattttt aactgataat cacgataaat
    26221 ttaaaaataa ttgattcttc aaataacttc tctacctgca ataatgaagt aaggagagaa
    26281 tcaaatggca acatttttta gttataaatt acaaaatggc gaaaaatatt ggggatttga
    26341 aacttattta gggaaagatc ccaagactgg aaaagatata cgtacaaagc gtagaagatt
    26401 caaatcaaaa gctgaggcgc aaacagcact taatagacta cttgtagact ttaaagaaaa
    26461 acaagagttt aaaatcaatg ttacaacttt tcaagagttg tatgaattat ggattgataa
    26521 ctacagaaca agagtaaaac cgtcttctgt tgccgttgct caacgttttt gcaaaaacca
    26581 tatattacct gcttttgggg aattacgatt ggataaaata agtgttagct attgccaaaa
    26641 acaggtgaat gaatggcaca aaacattaaa acaatatggt ttcttgagaa aaacaactgc
    26701 tcaaatattt aaatttggag tagcaatgga agtctgtaag gataatccta tgtctaaaac
    26761 attgcttcca agaaaaattg aagaagaaca acccttgaag ttttacacga aagatcaatt
    26821 acaactgttt ttacagaata ctaaagaaaa taattcagtt aaactgtaca cttttttccg
    26881 tttattagct tatacaggaa tgcgtaaaag cgaagcattg gcgcttcaat gggaagatat
    26941 agactatttc aataaaacaa taacaattgg taaaactatc gctcaagacg agtttaatca
    27001 agtagttcta caagtaccta aaactaaaaa ttcatcaaga acgattcagc ttgatgattt
    27061 tacacttaaa caactgcgta tttggcaaca agaacaaatg aaaataatga tcttatatgg
    27121 ctataacacg aattcaccaa aacagtttct tttcactaca aacacaaaca agttgtacta
    27181 cctccaagta gtaaacgatt ggttagactg gatttacaaa aagacaccga tggagcctca
    27241 aattactcca catggattta ggcataccca ctgtagctta ttgtttgaaa gtggtgcgtc
    27301 gataaaagaa gtacaggaac gattaggtca taaagatatt aaaactacca tgaatatcta
    27361 tgctcatgtc acaccacaat ctgttaagaa aactggtgac cgatttgcaa aatttatggg
    27421 cgaataattc caaacgtagc caaaaacgta gccaaccatt aaaatacaaa taaaaaaacg
    27481 ctcgaaacgc tttaata
//
LOCUS       DAYLZO010000022        26904 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.022, whole genome shotgun sequence.
ACCESSION   DAYLZO010000022 DAYLZO010000000
VERSION     DAYLZO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.022"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..308)
                     /locus_tag="ACGVJG_000893"
     CDS             complement(<1..308)
                     /locus_tag="ACGVJG_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFJ6556869.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     "
     gene            456..2009
                     /locus_tag="ACGVJG_000894"
     CDS             456..2009
                     /locus_tag="ACGVJG_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556870.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            complement(2117..3199)
                     /gene="uxuA"
                     /locus_tag="ACGVJG_000895"
     CDS             complement(2117..3199)
                     /gene="uxuA"
                     /locus_tag="ACGVJG_000895"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFJ6556871.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3190..4836)
                     /locus_tag="ACGVJG_000896"
     CDS             complement(3190..4836)
                     /locus_tag="ACGVJG_000896"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFJ6556872.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5020..5682
                     /locus_tag="ACGVJG_000897"
     CDS             5020..5682
                     /locus_tag="ACGVJG_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6556873.1"
                     /translation="MKSSSQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5826..6389
                     /gene="ahpC"
                     /locus_tag="ACGVJG_000898"
     CDS             5826..6389
                     /gene="ahpC"
                     /locus_tag="ACGVJG_000898"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFJ6556874.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            6399..7928
                     /gene="ahpF"
                     /locus_tag="ACGVJG_000899"
     CDS             6399..7928
                     /gene="ahpF"
                     /locus_tag="ACGVJG_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFJ6556875.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            8085..8435
                     /locus_tag="ACGVJG_000900"
     CDS             8085..8435
                     /locus_tag="ACGVJG_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFJ6556876.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            8534..9064
                     /locus_tag="ACGVJG_000901"
     CDS             8534..9064
                     /locus_tag="ACGVJG_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFJ6556877.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            9218..10369
                     /locus_tag="ACGVJG_000902"
     CDS             9218..10369
                     /locus_tag="ACGVJG_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFJ6556878.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(10478..12517)
                     /locus_tag="ACGVJG_000903"
     CDS             complement(10478..12517)
                     /locus_tag="ACGVJG_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFJ6556879.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(12575..13330)
                     /locus_tag="ACGVJG_000904"
     CDS             complement(12575..13330)
                     /locus_tag="ACGVJG_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFJ6556880.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            complement(13422..14528)
                     /locus_tag="ACGVJG_000905"
     CDS             complement(13422..14528)
                     /locus_tag="ACGVJG_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFJ6556881.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     regulatory      complement(14702..14868)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(15093..15299)
                     /locus_tag="ACGVJG_000906"
     CDS             complement(15093..15299)
                     /locus_tag="ACGVJG_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556882.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(15315..15929)
                     /locus_tag="ACGVJG_000907"
     CDS             complement(15315..15929)
                     /locus_tag="ACGVJG_000907"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFJ6556883.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(16076..16759)
                     /locus_tag="ACGVJG_000908"
     CDS             complement(16076..16759)
                     /locus_tag="ACGVJG_000908"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFJ6556884.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(16868..17326)
                     /locus_tag="ACGVJG_000909"
     CDS             complement(16868..17326)
                     /locus_tag="ACGVJG_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFJ6556885.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(17410..19173)
                     /locus_tag="ACGVJG_000910"
     CDS             complement(17410..19173)
                     /locus_tag="ACGVJG_000910"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFJ6556886.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(19394..20854)
                     /locus_tag="ACGVJG_000911"
     CDS             complement(19394..20854)
                     /locus_tag="ACGVJG_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556887.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(21062..21988)
                     /gene="glsA"
                     /locus_tag="ACGVJG_000912"
     CDS             complement(21062..21988)
                     /gene="glsA"
                     /locus_tag="ACGVJG_000912"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFJ6556888.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(22011..23438)
                     /locus_tag="ACGVJG_000913"
     CDS             complement(22011..23438)
                     /locus_tag="ACGVJG_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFJ6556889.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(23469..24683)
                     /locus_tag="ACGVJG_000914"
     CDS             complement(23469..24683)
                     /locus_tag="ACGVJG_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFJ6556890.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(24769..24996)
                     /locus_tag="ACGVJG_000915"
     CDS             complement(24769..24996)
                     /locus_tag="ACGVJG_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556891.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(25024..25266)
                     /locus_tag="ACGVJG_000916"
     CDS             complement(25024..25266)
                     /locus_tag="ACGVJG_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFJ6556892.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(25298..25855)
                     /locus_tag="ACGVJG_000917"
     CDS             complement(25298..25855)
                     /locus_tag="ACGVJG_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFJ6556893.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(25868..26056)
                     /locus_tag="ACGVJG_000918"
     CDS             complement(25868..26056)
                     /locus_tag="ACGVJG_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFJ6556894.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(26069..26635)
                     /gene="amaP"
                     /locus_tag="ACGVJG_000919"
     CDS             complement(26069..26635)
                     /gene="amaP"
                     /locus_tag="ACGVJG_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFJ6556895.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         8001 a         5267 c         4664 g         8972 t
ORIGIN      
        1 ttctcgtcat agccaatcga caaagcggga tcaacattct caaatgtaac agctgattgt
       61 agtataggtg ctaagtctcc tgaatagtca atttttgctt tgatggtcgc tgtttgtcct
      121 tttcttaatt ttgcgactcc tgaattgaag gatgaaaatt catcctgaaa ggtaattcga
      181 ttgacgttgg cccacttggt ttctgtccat ttggctgttt ctatctcctc tgtgtggact
      241 gtctgattta tagtaagcga taagcttaag gctgcaaatg ttgatattaa gatggttgaa
      301 tatctcataa ctctctcact ccttgatttt tttgtatgca agaaataaga tagtagagat
      361 tctacctata tgtttactgt ttttttgtct aaatttatga aaaaaacagt aatattttca
      421 agttttaaca atttaagtaa aaaggagcct ctttcatgca aactcaagaa gcgattaaaa
      481 cgtttctctt gaagaaaaaa agcaatacaa aattagatat ttttcttttc ctatctgaac
      541 atcgtttctt tattactaca caatatttag cagatcattt ccatatgtct gaatcaaatt
      601 ttttactata catcaaagaa ttagaacaag actttgagcg tctcaatcta acagaactgc
      661 atattgataa gcaaaaacct tttttaaagt tgaattttga aggaattgat ccggcttact
      721 gctattatcg tctatttgga agatactgca acgaatcagt cagttatcag atcttgactt
      781 ctttgttcag ctgtcagaca aattcgatta tcagtttaag tcagcaaaca aactatagtg
      841 cctcttattt gtatacgaaa atgaaaaaga ttaatgcatt tttagcattg tacggtttgt
      901 ctatctcctt ttctaataat ggcagaaagt ctttttctgg aaaagaggta caaattcaat
      961 atgcagcatt agatatttat tggaacatct tttcaagtac tgctacagaa ttttatgatg
     1021 aagatccgca agtagtggct tttatgatga atactttttt taaaaaagaa gtacttgacc
     1081 gactaaatag cggtatgata gataaattat attttctatt aaaaatatgt aaagagaatt
     1141 ttcctgcaac ttctgtgaaa gacgtacaaa aagaattcac agagtatgaa tatattgact
     1201 tttttattga tccctctgta gatattttgc gacctaatct gccaatttgt cgcgaacaac
     1261 gcatcttagt gaatatttta gctcgacttt ctatcacgaa aatcgaatcg gaagaaacaa
     1321 gcttggagca atacaaactt ttaaaaaaag caaacgttcc tcatgttctt tattcaagag
     1381 aaatggtgga gtccttttgt actgtatttg atttgtttat acctgaaaaa gaaaagattc
     1441 tttactcatt ggcattcgca cgaaataaac tttataatgg atttcttaac ttcaatcaac
     1501 caaatacgcc ccttcctact ttcatgcttt atcaagaaca tagcgtgttg aaagaagtcc
     1561 aaaccgccat tgaacaattt tatatggatt tcagagaaca aaatcaacac ttgttaccag
     1621 ttattttaga aaaggaaaat gtgcaatgga tggttgaaga cctagtacat ttgtatgatc
     1681 gctacaaaaa acagccgcga attgtgattg gtgtgaacta tacgcgtgat ttttatatca
     1741 gtaaagatct gatcgttaag atagaacaaa ttttttcaaa tgaatcgatc aaaatacaaa
     1801 atgattttat ggagaactgc aacatcgtta tcagcgactg ccctttgaaa caattgcctt
     1861 ctcatatcaa aaaaatctat attttaaaag gaatcatcac accagaagac tggaaaaaag
     1921 tgattatgag gatctcagaa tatatctttg aattaaaaga agaagcactc atggatcaat
     1981 cgaaaaaaga aaatcaagtt acttcctaga ccaaataaga tacataaatc cgaatgctta
     2041 ttaacaacct ccggatttat gtatcaagaa tttgaacatc cttcttattt acataataaa
     2101 attacagttt ttttcactaa ttatctttac ttttgtttcc tttttcgatt gcttcccata
     2161 atccatttag ataaacagca cctaatgctc gatcatataa gccgtatcct ggtcttcctg
     2221 tctcattcca gatcattctt ccatgatcag gacggatgta ttcgttaaat ccgttgtcat
     2281 acattgtttt tagtatgcgg tacatgtcta atgaaccgta ttcggattta tgcggtgcct
     2341 catagaaatc tcgattagct aaaaatttaa tatttcgaac atgtgcaaat gggattcgat
     2401 ctctttgaca aaattctgct aaaatcccat atacatcatt ttctggatct tctgcaatac
     2461 tccctgtaca aagagtgatt ccgttataag ggctgtctac tatgcggcac aaccaatcta
     2521 aatcttctcg attctttacg attcgaggta atccaaaaat cgagtatggc ggatcatctg
     2581 gatgtacagc cattttgacc cctacctctt cacatgttgg gatgattgat tggagaaaat
     2641 aggcaaaatt ttctcgaagt ttttcttcgt ctactgatgc ataggcttca agcaaggatt
     2701 tgatatgagc taagcgctca ggttcccatc ccggcaattc aaattcattg ctggaatgtt
     2761 cgatgcgcgc gacgatttct cttggatcat ctggaatatc atcagatata aaagctaacg
     2821 tggatgagcc gtcttctaag cggtagtcaa ggtcagattt cacccagtca aatacaggca
     2881 taaaattata gcagatgact tctattccat attctgccaa gtttcggatt gtctgcttat
     2941 agttttcgat atactgctca cgggaaggta atccaatttt aatggcatca tgcacattga
     3001 cgctttcaat tacttttagt gttaacccac gagactcaat ttcttctttt agttctttga
     3061 tcctctccct gggccatacc tctcctactg gtatatccat caatgtgcct acaatccctt
     3121 tgaccgcagg aatctgtctg atttcatcta aggtaatctt ttcttcctgt gatccgtacc
     3181 atctaaaagt catttccatg ataaatttcc tccatgttct tgtaagtaat cctgtaatgt
     3241 ttttcgtacg gcattcggtc ctgctaccat cttgatgaac atttcttcta tttttctgcc
     3301 aagctctgct tggtaaagat ctacaccaaa aatttcttta tttgacaaaa taggagccat
     3361 tttctcatga atatgtgatg tatcttgcgt acccagttga atatcggaaa gcttatgctg
     3421 aaagtctgtc aatagtgggt ctggactcgg gatgaatggt tttccttgat catcaatgcc
     3481 aataaggtat cgaagccaag cagcgagagt caatggtatg aatacaagtg tctgcgggtc
     3541 tttaacttct atttccatat attttttaat cgtctcacca taacgaatcg ccaccttttg
     3601 agaggtatcc gtggcgattc gctgaggcat atcaggaata tttggattag aaaagcgcac
     3661 agtaattact tcttccagaa agatctttgg atcaatgata cctggagact ccacaactgg
     3721 gaggccttca ttgtacccga tttcagaaac tagcagcgat aaatctggat ctttcatctc
     3781 ctcagctatg ctcctatacc ccagtaaaca acccataata gctaaagacg tatgcaaagg
     3841 attaaggcaa gtcgtcacct tcatcgtatc tgctttatct accgttgcac gatcagtcaa
     3901 gaatacccca gctttttcga atttcggacg tccattggga aaatgatctt ccatgattaa
     3961 ataatggatg tcctctgtat tgacaaatgg agcaatatta tttccttttg cggtttggaa
     4021 aatcgacata tttgaaaagc cctcttttgc caacattttg ctaactgaca aggaaggatt
     4081 cggggtgata cgatcaatca tcgtccatgg aaacgcaaca atttcttcat tcgataagta
     4141 ttggataaat gtattttgta cataaccttc cttttgccat tcttttgcga tcgttagcaa
     4201 agaagtacgt aattgactac cgttttgtga aaaattatct gtagagacaa gtgcaatcgg
     4261 ccaagctcca gataaaaaac gtttataaag tagcgcagca agaatactca ttgtatgtga
     4321 tggcttcttt ggaccatttt ttatatcgac tgcaatcggt cctatatatt tattatttgt
     4381 atctttcaat cgataacctt tttcagtaat agcaaaagta actaattgta aactaggatt
     4441 ttcaaagtgt cgaatcatct tatcaaagtg ttcagaacaa tctggatgac aatacagact
     4501 gtcagctgtc gcagctaata tacgtttgac gaactctcca ctgcttggca tgaccacttc
     4561 aagaatcaaa tcatcaaaat cttggtaagc cctttcaatt atttcttcat cgaatgtttc
     4621 acaaacaatg acaccagtgg acaattcgcc tagatccgct aaacgttgag cgatttctgc
     4681 atgaaatcct cgataaagat ttccgccacc gaaatggatc cagacaggat ttttttgtcc
     4741 attttctctt aatgatgcac ggtcaaaatg aggtatttct acttcttttt gagataattc
     4801 agggataaag ttcctccagt tgtttaaatc aatcattttt cccctcctaa actaacatat
     4861 tagtttttgt attcaagtga attttataca ataaaaatcg ctatgtcaac caaaatttta
     4921 ctatttatac aaatttacta gttaatgtga cataccagat tgtttttcag acctcttctg
     4981 ttatactacg ttcatagaaa tgattttgga ggaataaaga tgaaaagcag ctcgcaatca
     5041 caagcttatc aagcgattcg taaacaaatt atctatgctg acttagaacc tggaaggaaa
     5101 atatctgaaa aggcactgga ggaacatctt gctatcggcc ggactcccat tcgcgaagca
     5161 ttgattcaac tacgtcagca agaactcgtg tatacgatac ctcaaagtgg tacgtatgtt
     5221 tccaaaatta atcttgtttc tgcagaaaat gctcgctttg tccgagaaca gttggaagag
     5281 caaatcatga ttgaatgttg ttccaaattg gacacacaaa cacgaatagt gctagaaacg
     5341 attttagaag aacaagaaaa agcagtcaaa ataaatgacg aacgcgcttt tttccgtgca
     5401 gataatttat ttcatgaagc ttgtttcgaa attgcaggaa gaaaagaagt ttggaactgg
     5461 ttagatgacc ataataccca tttagaacgt ttccgctggt tacgagtgac cattcagggg
     5521 cttccatggg aaacgatcat ggaacaacat tatgatttac tcaaggcttt agttgataaa
     5581 aaaccagaag aagctcgatt tcaaacggga ttgcacttac acatgatgct gaaagaacag
     5641 gaaactgtta ctcaacgtta tcccgagtat ttccagcagt agcattttct tgcgacctct
     5701 tctattttta taattaacct tcgtttttga gtaatcttta ttaaataaca aaatatccat
     5761 tgactttgca actccaacta aattacaata attataaata aataattatt attggaggtt
     5821 ttactatgtc attgattggg aaaaaaatcg aagaatttaa tgcacatgct tatcatcaag
     5881 gagaattttt agaagtcagt gaaaaagatc ttatggggaa ctggagtatt ctttgcttct
     5941 atccagctga ttttactttt gtttgtccta ctgaattaga agacttacaa gaacaataca
     6001 gtacattgaa atctttaggt gttgaagtct tttcttgctc taccgatacc cactttactc
     6061 acaaagcatg gcatgataca tcagatgcta tcggtaaaat cgaatacgtg atgatcggtg
     6121 atccgtctca tcagatttct cgtatttttg atgttttgga tgaagagcaa ggattagctc
     6181 agcgaggaac atttattatt gatccagacg gaatcgttca agctatggaa atcaatgcag
     6241 atggaatcgg tcgtgacgct agtgcactga ttgataagat tcgcgctgct caatatgtac
     6301 gtacccaccc aggtgaagtt tgtccggcaa aatggaaaga aagtggcgaa acattgaaac
     6361 caagttttga tcttgttgga aagatttagg tggtggatat gttagacaaa gaaactaaac
     6421 aaaatctcga acaatattta gcactgattg aatctcctat tgttttttct gttagtttgg
     6481 ataatagtga aaatagtcaa aaactagctg aatttacgaa ggaaatagca gaaatgtctc
     6541 ctaaaatctc ttgggaaaaa agagattcga tgcgcacacc tagtttttct atcaatccag
     6601 ttggcaaaga aagtggaatc gtttttgctg gtatccccct cggtcatgaa ttttcctctt
     6661 ttttactggc tatgttacaa attagtggac gggcaccgaa aatcagtgaa tctttgagtc
     6721 ttaaaatcaa aaaaataaca caaacattgc attttgacag ttatgttagc ttgacttgtc
     6781 ataattgccc cgacgttgta caggcattga atatattagc tgtattaaat ccaaatatta
     6841 ctcatacgat gattgaaggt gggatgttcc aaaaagaaat cgatgataaa aaaatcatgg
     6901 cagtgccaac tgtgttttta aatggagaag aatttagtag cggacgcatg acgatcgaac
     6961 aaattgttga aaaaatcact ggccctttga ttgaagaaga gttgtttgaa aaagaacctt
     7021 atgatgtatt agtaattggc ggtggaccag cagctagtag tgcagctatc tatgcagcac
     7081 gtaaaggaat ccgtacagga cttgtcgctg attctttcgg cggacaagtc gttgaaacac
     7141 tagggattga aaatatgatt ggtacctctt acacagaagg tccaaaattg atgcagcaag
     7201 tagaaagtca tgtgcgtagt taccctatcg acattatgat gaatcaacag gccgtttcct
     7261 taaataaaga gcaacatttt attaatattg gcttagctaa cggtatatca cttaaagcaa
     7321 aaaccgccat catcgcgaca ggtgcgcact ggcgttctat caatgtacca ggcgaaaaag
     7381 agtttaaaaa taaaggaatt gcttattgcc cacactgtga cggcccttta tttaaaggaa
     7441 aagagattgc tgttatcggt ggtggtaatt ctggtgttga agcagcaatc gatttggctg
     7501 ggttagcaaa acatgtatat gttctagaat tcttaccaga attaaaagca gatcaaatct
     7561 tacaggataa actagtctct cttgcgaatg tcactgttat caaacatgca gcgacgaaag
     7621 aaatcagcgg tacgaatcag gtagaatctc tctcctatat cgatcgccgt acgatggaga
     7681 tgcatacact agctgtttca ggagtattca ttctagtcgg cttactccct aatacggatt
     7741 ggctagatgg aacgatcgaa atgaatccaa gaaatgaaat agtgaccgag aaaaatggcg
     7801 caacgaatat ccccggcgtt ttcgcagctg gtgattgtac agatagtcca tacaaacaaa
     7861 tcattgtttc aatgggttca ggagctactg cagctttagg agcattcgat tatctgatgc
     7921 gcacttgagc tatgaagatt gtaaaaagtc agagagcgga aaaaaggatg gtgacaagaa
     7981 ttttgtcaca atcctttttg tttttttgaa taatcgcttt ttcttctctt tttcttatat
     8041 actataagta gtaaataatt tgaaaaaaag aaaggtgact gattatggcg caaaatcgta
     8101 tttttacgac aagttttgca agcgtttatc ctatgtatgt agcaaaagcg gagagaaaag
     8161 atcgtataaa agaggaagta gatgaaatca tttgttggct gacgggttat tcccaaacag
     8221 aactagaata tcaactacaa aaacaagtga ctttcgaaca attttttgcg gaagcccctg
     8281 tccccaatcc ttcacgttcc ttgattacag gtgttgtttg tggtgtacga gtagaagaca
     8341 tcgaggataa cttgatgaaa gaaattcgtt atttagataa aatgatcgat gagctagcaa
     8401 aaggcaaaaa aatggaaaag atcctcagaa gttgattttt ccagagatat tcttctcctt
     8461 cgctcatttt gtaacattta atttggtaaa atgactagtt cacgttaaaa taagtcaaag
     8521 gaggttttct tatatgtttg agacgattaa acaaaatatt caaaaatacg ccttattaag
     8581 agctgctgtt tatattattt ctgggattgc gattgtgcta aacccttctg ctgtttttca
     8641 ttttatcggt tatctgatca ccgcctatct tgttttgctt gggttgttga atctttttga
     8701 ggcttaccgt tttaaaagga aaacaggttc ttggagtatc ggtctgttca gtggtctttt
     8761 tttcctgatc ttagctctga tcgtctatgc ttttgcacct gcaatcgttt ctatcctgcc
     8821 gattcttctt ggattagctg ttatcatcaa cagcttgttc cagttgttct tcagcatgaa
     8881 tacaaaagct aaaagttgga gtgtttatag tagtattctg ttgttgattg gcggattcgt
     8941 cttattgttc aatccattca aaagtttgat gcttctattc caaatattcg gctttatttt
     9001 gattttcatg ggcatccagg agattatcaa tttcatcaga ataagaaaaa tgactatacg
     9061 ttaatcattt ttagttatta aaatagaccg tataaaaacc aacaatccaa aaaaatcttt
     9121 tggattgttg gtttttttgt gagtatattt tcctattttt tcaataatgt atgttatgct
     9181 aagttcgggc tatattttat acaaaggaat gatgagaatg gaatttattg gtattttatg
     9241 cctgatattg atttcaacaa cgattggctc acatatctcg cggagattag gtatcccagc
     9301 agtgattgga caattacttg ttggtgtttt gctaggccaa gctggattga attgggtcaa
     9361 acctgatatt ctagtccatg acttttcgga gattggtgtg atcttattaa tgtttttagc
     9421 tggtctggaa agtgatttat ctcttttgaa aaaatatttc cgacctggta tgttcgttgc
     9481 tttgcttggg atcatcttcc cggttgtttt tggctggttg acaggcaaag cattccaagt
     9541 ttcgggaaat gaagcgattt tctttgggat tattcttgcg gcaacttctg tcagtatttc
     9601 agtagaagta ttaaaagaac taaacgtagt gaatacaaaa gaaggatcaa caattttggg
     9661 tgcttctgtg gtagatgaca ttttagtcgt tttagttcta agtttcagcc tatcttttct
     9721 aacgggggaa gcttctagca atgcttcttt accgcttgtt ttgattgaac agctaattta
     9781 ttttgttttt attttcttat tagtcaaatg gattgcgcct ttcctacttt catttgcaga
     9841 aaaagtgtac gcaaattctt ctattatcat cgtatcgcta gttatttgtt taggaatgtc
     9901 ttacgtagcg gatcttgttg ggcttagctc tgtcattggt gctttttttg ctgggatagc
     9961 tgtcagtcag acaaaggtta aagaagaagt ataccacaat gtagaggctt taggatatgc
    10021 agtatttata ccagtatttt ttgtcagtgt aggattagaa gttgattttt ctgactttag
    10081 tgaacaacta ctctttattt ctatattgac gcttgtagcg attttgacca aattgcttgg
    10141 aggttatttt ggtgctcaga ttgccggttt ttctcaacat agttcattga tggtcggtgc
    10201 tggtatgatt tcgcgtggag aaatggcttt gatcatttta caaattggac agcaaagtca
    10261 attgatcgaa ccacagtact attcaccttt agttatcgtg attttattaa gtacacttat
    10321 ttctccactt gttctcaaat attttacaaa aaaagtttat gaagtgtgag taaatcaaaa
    10381 agaagttgtg aaagagacat cgtgaaaacc gtttatttga aaaaaggact gtgacgataa
    10441 cgccacagtc cttttactgt acttaaaaag caatttttta ttgataattt tggttgaggt
    10501 attgcaatag ttccgatgct cgtttggttg ctgaatcatc atcttcttta ttttccaaca
    10561 tgctgaccat catataacct tggttatcaa gattgaaagc aaacaagaaa ctgttctctt
    10621 gcccctttac atcctgtttt tctttgattt ccgctgtacc agttttcgct gccaacggaa
    10681 tccctaaagc agaaagagaa tgtgctgtac catttacatc ttgcacaact tctctcagat
    10741 ctggcacgat cgtttgtact gctgtttcgc tgatgacatt cttcttatct tttgtctctt
    10801 tatctgcaat caatttagga tagacaagtg tgccattgtt ggtaaaaaca gaatacattg
    10861 ctgcttgctg gataggatta attagaagtt cgccctgtcc atatccagta tcagctagca
    10921 agatatctga gttaaagcta tcttcattag aaatttgtgc tggattcata ctaattggca
    10981 aatcaaggtc ttcaccaaaa atgaatttat ctaagcctgt acgaaatttt ttctcaccca
    11041 ttttcaacgt ttcttgtgcc gcatatatat tatcggaata gatcaaagca gtttttaagt
    11101 ctacttgtga tacatcacta ctgacacgcg ttgtttgaaa agatccccaa gaactgtctt
    11161 tttgccattt aagcccgttg atcgtcaaca cttcatttgg atcgatagtg ccgttgtcga
    11221 gaccgattgc tgctgtaatt attttaaacg tagatccagg agcataacct gtcgcaaatc
    11281 ggctgatgaa tggatgttca ggattttctt cataagcttt gtaatcttct tgtgagatcc
    11341 cgtttgtcat tttgtttgga tcatagcttg gagagctagc aagcgcaaga agatcaccgg
    11401 tttttggcgt tgtcgcaaca gttgatccag cttttcctcc tagactgtca aaagctgttt
    11461 tttgtgcctt cgcatcgatt gtcaatttga tatctttgcc attttgaact tcatgttcga
    11521 tcagaacttt tttctctaca ccgtctgcat ctgtaatgct taatttccca cctgttgtcc
    11581 cacgaagatc cttatcaaaa gccatctcca aacctgagcg accgattttt ccattactgc
    11641 ttaattctgg attcttatca atatcttctg ctgtgatatc tcccacgtaa ccaatcagct
    11701 gagcagctgc ttcacccaaa ggataatatc tgccgtctac ttcttgaatg gtagctccag
    11761 ctggaagttc tggcgttgct ccatcaatga ttttcaatgg gacaaagtaa tcgggttgta
    11821 cccagctttg tgaaatcgcc tgattgatag catcttctgt taagtcgaag gaggaagcaa
    11881 tcgctttgat attggctgtt ttttcgcctc catctccaag tttacttgga acgactccta
    11941 attgttttag tttgcctgtt gttgctaatg gttcaccatt tcgatctata atattccctc
    12001 ttgctgcttc ttgtgtggtc aggcttactt tgtcatttcc ttccatttct ggaaaaacca
    12061 agttgggttg ccagttgatc gtggtttgac catcatttct gtctaatgtt cctttataag
    12121 aaagatcttt caattcacct aatgaggtat tcatctttgc tttatatgaa aaactgtaag
    12181 tagaatcgtc ttttttatct acttttagat tcgatatctg aagtcctttg acatcagcag
    12241 caccgtatat attctggtat ttatctaaga tctctttttc agataatgca cttttatttg
    12301 ccgccttttt tgatgtcatt tctgcagctt tgttataatc tcccttgttc aaagcttgga
    12361 caaattgctc aactgtcttt tctccagctt ctactgcttg ggtttcttga tagtgctggt
    12421 agtaaaaata accgccaccc gcagctgcta ttaagatcac tgcgccggca atataagcgc
    12481 ctgttcgatt tttgccgtgc ttgtcacttc ttttcatttt ttcctccttc tcttgcttgt
    12541 cattccttta tttcactatt tatacctgtt tattctaaca tatgtctttg aggaaatcca
    12601 taccacaatc cagaagcaca ctttctggaa ggttcgtttg gatactacca agtaatttcc
    12661 caaccgcttt gggtagcttg aatattgaag ttacgtcttt cagctgctga gagatagctt
    12721 tgatcacttg ctgctgcctt ctgatcctcc cgaagtctcc ttctttgtct tcccgaaatc
    12781 gagcatactg gagtaaagta ttaccatcca tcacttgctc tcctttgttt atatatacgc
    12841 catctaaatc tagagatttt tctgcatcga tcttgactcc accaggaaac agttcattaa
    12901 cgcaatcgat aaaatcttga aatgtgatac ttgcataata gggagcttca aatttgaaat
    12961 tttcctttaa tgtttgattt aataaatcaa ctcccccata agagtaagct gcatttatct
    13021 tattcattcc gtaacctgga atatcgacat atgaatctct catgatagag atcagttttg
    13081 cttgttgtgt tttctggttg tattgtgcaa ccagaagtga atccgagcga ccgttttcgt
    13141 ctttcgaacg agcatctgaa ccaatcaaag ccacatttag tgtggtaggg atacttttcg
    13201 gctctacctt tttcacagtt gaactctgtt ttgcagacga aaatccatgg ataaactcaa
    13261 tcgaaaaata gccaactact aatgtaagca gtaacaaaat agataatatg attttttgag
    13321 atactttcat tgcgaattcc tctttctaaa agaacctctc gcttgaaaaa taaaagattg
    13381 tactttatga taattcaaac tgagagtttc tcagtttctc tttattgttt ttcaggtcga
    13441 tagagcgcca actcttcggc ctgtatcttt gaagaaatat tcaacgtgat tccgattcct
    13501 aaagaaagga tcagataact tgttcctccg tagctaacaa atggcaatgg cacaccagtc
    13561 attggcatca gccccgcgat acttcccacg ttcattatcg tctgaacgaa tagcaaagtt
    13621 ccgaatccta aaagaaacaa tccagcttgc tgatttttac agcgactgct taaacaaaag
    13681 attctcatgc ataatgagaa caataagaaa agcacacaca atgcaccgat tagcccaagc
    13741 tcttctgtaa taatcgaaaa aataaaatct gtttctgtct ctggaagata cccttttttt
    13801 gtgatactat ttcctaatcc ccgcccaaat atgccaccat tatatagtgc ataaaacgaa
    13861 tgggtcattt gatagcccgc accatgacta tcgataaaag aatctcttaa agtcgcgatc
    13921 ctttcataag catgattaaa catctgtgga agccaacctt tatttcctaa atataatacc
    13981 ccgcctgctg ctccaatcag caatgcagaa aaagtaacga ttagatagat ccctttttta
    14041 aatggaaccg ccgctgccca aaatatgacc cacgcaatcg aaaggatcat caatgctcca
    14101 gcaatctttg gctgaaataa aatcagaaaa gttataccta ctgtaatcag gaatggtttt
    14161 tttagatctt ttgggctact atttccgtca cgaaaaaacc agcttaaata aaaaatcaaa
    14221 aaaagatttg ccagttcaga aggctggaat tgaatgccaa acagagagat ccaacgttgt
    14281 gcgccgttga cagtgacacc gaatatccct attcttacta atactaaaaa gaaaatcgat
    14341 aaggctaaac cgtatcctgc tattttaggg tgaagtaaat agtgtagttt gactgaacga
    14401 gctaagaaga tcacgctcca actcaaaaat atgaaaatta gttgacgcaa taatagactt
    14461 tttgtatttt catcggcttg caataaacga taagaactgg cactgtatac ttctaaaaga
    14521 ccgatcatgg acaaagtcaa ataaggaccc aaaatccacc aatcgatctt ttttcaccgc
    14581 atctcctcct cacacagcaa tccacttgtc aggtgaggag cgagtttgtc tcatcaaata
    14641 agtcagtcac aaaaatataa agaaaaggtc agaaaccttt tcgaacaaga attctgacct
    14701 agcatcatct catcgtccga gttttagcac tatacaacgt aaagaaaaca ttcttagcta
    14761 ttggaaaaag ccttaatcca acagtttctg ttttacccat tggcgacttt cgacgtttct
    14821 gggcaacagc ctatgtttgt ataggagcct cacctaacaa ggttattact atattctatt
    14881 tacgagttca tgatagactt ttcctaatga ttaggcaatg gaatttgaaa tgaaatagtg
    14941 aaatttaaca atctttaata ttttttagaa aaaaataaca aataagttga aagaatcccg
    15001 ttgcaaattc tttcagttgc accaggattc tttgataatt ttttagtttt atgtgttttt
    15061 ttatatgttt tttcactttt cggaataaat atttatttgt taaagtaatt aaacaatccg
    15121 ctaatcgttt gatctggcat ttctatttcc ttaccatcta aagtaacagt agtaacgaca
    15181 aaatggtctt gcaatggcgg aacttctaag tactgccctt gcagaaaatc attcactgtt
    15241 tcatattctt ttttcatttc ttttgaatct gtcgatggtt tatagcttat ttttaacata
    15301 actagtgctc ctttttattc gggacgtgca atcgtgaaaa tttctcctat tgctgcataa
    15361 tcatttcctg ccaaacggct tacagcattc aattttcttg gatcgatcct tcctgtctta
    15421 tcatcataga ctgtttcatt taaatgatat ttctgtactt ctagcaaaaa taaatcagct
    15481 gttactttat ttttattccg tatctcgaca tgttcatata aggatgtttc aaaacgaact
    15541 tttgcttctg tcaatgctgg tactgaaatg attactgaat cagttaaagt caagtcagtc
    15601 aggtacagtt cgctttcctc attaggcaga ttggctgctg ttttatttac ttcttctacg
    15661 ttgtcctcat caaggatatg gataacagct tctcttgtct caagaatatt ctttgctgtg
    15721 tcttttactg catcttgttt tctctggatc gccaaggata agatgggtcg attcgaactg
    15781 acgatattga agtaactgaa gggtgcgata ttcactatat cttttgctga tttagtcgtt
    15841 actactgcta ctggtctagg gatgactgaa ccaatcagca acttgtaatt ttcacgttct
    15901 gtcaattctg aaggactccg ttcaaacatg gtttcgcctt ctttctataa aattcgttaa
    15961 aaagcagatc ttgaagacaa aatgaaaagt aatgcttttt tcatcttctt agaatctgct
    16021 ttaaactatc tatcaatttt tcttttttgt caagaatctg tgccgtgcgt gttttttagt
    16081 tatttgcttt acttgcggct tcgacatatc ttttagtgat caattgcgga cgggtgataa
    16141 tagatccaac tacgactgaa aaagcaccta attcatatgc tttctctact tgttcaggag
    16201 agtcagtatg tccttccata attactggga tatcgacttg ttctatgatt tgattcaata
    16261 aatcaaaatc aggtgtctgc gtctttcgtg aatattctgt atagcctgct aaagtcgtag
    16321 acaaaagatc aaaaccatct tcttgcgctt gaagcgcttc ctcaaaagtc gaaatatctg
    16381 ccatagctaa ccgaccgtgt tcgtgaatca tttggacaag ttcagcagtt gttttttctg
    16441 tcggacgttt acgagaagtt gcatccagtg caatgatttc tgcttgcgtc tccaaaagtt
    16501 cttccacttc tttttctgtc gcagtaataa acacttctga cccatcgtaa tctcttttga
    16561 taatccctat taccggtaaa tcaacgattc gcttgatttc attgatatct tctgtcgtat
    16621 ttgctcgaat accaactgct ccgccttctt ttgcagccaa agccattctc cccataataa
    16681 aagaactgtg tagcggctcg ttttccaaag cttgacaaga aacgacgact ccgcctttga
    16741 tttgttccag tgtatccaca atatctcctc cagtaatgtt atataaaaat acagcttcat
    16801 ataaaaatgt aacccttttc tattcaggag acaataatga gctatttctt tcagcagacc
    16861 tttcttacta aaagaaaatt acttcaaatt cttcgtatac gaccagcttc tcttctgtaa
    16921 attctatgtt caacgcttct ttataagcat gtttaaaaaa ctcagcatgg actttccgcc
    16981 aataagctaa tgtccgatcc ccttctcctt ctttccaagc atgctctttc gtgacttcac
    17041 taaaaggcaa gatggtcaca tttatatttt tgatgataca aactggttca ttttttccat
    17101 tcaggaccac gtctaattga ccaacttttg gtaaaggctc attctcttgt ttatacagat
    17161 cgtaaccact tgatgtacca gtctttatct tttttacaac tagttcgctc aactcgtctg
    17221 ccattttttc tgtatcccca aatgaaaaga aacctaattt ttctgattga taactaggat
    17281 atttttcttg gaactttttg acatattctt ctacttgctg atacataaca tccacccctt
    17341 tgctttttat aatgaaaaaa ccatccagat actagtataa acaattatct ggatggtgag
    17401 cataatgaat tattgaatat tcaagacacg cagactatct tcaacgattt gttttggtgt
    17461 taaatgataa cgttcataaa gctgccctac aggaacgcta tccgtgaatt ctttctctgc
    17521 accaaaattt aatacgttca ttgaagaatc accataataa cgactgatat tttcgccaaa
    17581 tcctcctgac aaactaccat cttctaatgt gatgactaat tcgtgtgttt cttttaattt
    17641 atctaatgtt tctacgtcat tttctgtgat cgttcctgga ttgatcaacg ttgcttcgat
    17701 tccttttttt ctaaaagctt ttactgtttc ttttcctaat tcaagaaaac cgcctaaagc
    17761 taaaattgct acttttcctc cattatttac tgtttgatag gctggaatac tataatcatt
    17821 tgcgatagct gtaccatgtt ctacaccatg tgtaggaatt cggattgcta caggctgcgc
    17881 tgtttgttcg aacgcccaat ctagcataga gatcatatcc tcttttgttg ctggagcaag
    17941 atatttgatg ttcgggatac ttgataagta aggaatgtca aatgttcctt ggtgtgttcg
    18001 gtcactagct gagatcgttc cgccaccgac taaaataatc actggattct cgttgatcgc
    18061 taaatcatgt gataattgat catatgcccg ttgcaagaaa gtacttgatt ggaagacgac
    18121 tggacgtact ccctgagaag ccaaagcaga agcgaaagtg atagagtgct gttcagcgat
    18181 ccccacgtca aaataatgat ctggatgttt ttcttggaat gttttcagtc caaaggcgcc
    18241 tggtatcgct gcattgactg cggcaatagg agctgtactt gtttgcattt tcttatccaa
    18301 ataatccatg atgatcatat taaatgtttc tccagaagtt acttgctttg cctccccggt
    18361 ggacagatca aatggtacat gccaatgata tttcatttta ttatcgattg ccggctgata
    18421 accgcgacct ttttccgtat ggacgtgaag tacaatcgga tgatcaatat cttttacttc
    18481 ttcaaataaa cggatcaccg tttccagatc gtttccatta tccacgtaac gataatcaaa
    18541 tcccattgct ttaaataggt tattgctgct tttaccgttg gtttgacgaa gctcagctaa
    18601 gtttttatat aaactgccat gattctcatc aatagacatc tcattatcgt ttactaatac
    18661 gattagattg gattgtaaaa caccaccatt gttcaatcct tcaaaagcta agccaccact
    18721 tagggagccg tcaccgataa atgctaccac atttcctgat tctttcttca gatctctcgc
    18781 tttagctaaa cctgttgcta agctgataga tgtagatgtg tgccccactg taaaatagtc
    18841 atgtttactt tcatgttggt tggaatatcc gctcacttca cggtaatgtc ctttttcaaa
    18901 accatttttt cgaccagtta aaagcttatg accataagac tgatgggaaa catcccaaac
    18961 gattttatcc tttggtgaat caaatacata atgaaacgca atcgttaatt ctactacacc
    19021 tagattcgga ccaacgtgcc cacctacatc gctgtccttt tgcaagattt tttggcgcat
    19081 ctcttctgcc aactgtttca tttcctcaac agataatttt ttgacatctg cggggctatt
    19141 gattgcatcc aatacatgta atactgtact cataatacat tctctcctcc gtactatctt
    19201 cttttaatga aaatagattt gttacacttt atctattcta ctacttagag ttgactctaa
    19261 gtcaatagca ttttaaaaaa atttcagaga aagcttgtta tataagtaga tcttcgcacg
    19321 aatcaggact caaataaaaa ccttctacag acaattaaaa agctttgtag aaggtctcct
    19381 aattaaggtc tcatcaagaa atatgttttt ttaataggtg aagttcctga gcaatatgat
    19441 acaaatggat gtttgcacct tttaatgaga ttggatagat ctttgtcttt tcagtttcta
    19501 atccattttg atagctagca gcatcgcaca aaaccactct ttttttcgca catagacatt
    19561 catcaggcaa ttcttctaat gaacgaaata aaataggatt tatttcagcg atgccttctt
    19621 ccgcatacaa atgaaattgg gttcgataaa aaaattgttt gactgaactt tctgaaacaa
    19681 caaaaatttc tatttcctgt ctccccgctt gttcactgtt gatcaaagaa agacttttga
    19741 cgaattttcg aatgaaattt tcattccatc ttagttggat ttgatttttc tttttccact
    19801 cttccaatat ttctacaatt tttccgtaca gaagttgttc tttttctgat agaagataga
    19861 tcctttccgg taaaaatact tgtccatctg cccacaacgt acggacaaat tgtaaaaagg
    19921 attgcttgaa aataaaattt tctgcatcgt cgatttcagc gatactaata atctgtaaaa
    19981 tcaattcatt cagtgatagg tcattgagaa caaaattttt ataaacaact tcgaaatctt
    20041 ttcttaacag ctccatgttc tcattggtat aatttctact gttgaacaaa gaaaaaatat
    20101 aatagacatc ttcttcttgt aagtctagaa ttcctttttg ctcttccaat ccttgacaga
    20161 ctaattgaaa taacggcttc gtttctgcgc ttttcttatc ttcttctttc agtccaaggg
    20221 gaaatgcctt tcttccgatt gcgatctcta ttcctcgtat gataaaggac ttttcatctt
    20281 ccgaatatct tcttttaaaa aaatgagtct ctacataatc aatcaactcg ttacaagctt
    20341 cttctacata cggattttta ttttcatagc cttcccaatt gacataacgt actaaagcta
    20401 gcaagatgtt ccgcgagtca aattctggga taatgatttc tttatttttc aaataatcat
    20461 tttctttaaa aaaatccaat acgatttttt tgattgtata tactttgctc aaagaaataa
    20521 attcaagatc agccagttct gtactcgtga atataccatt aaaataataa cttaaaaact
    20581 ttaaaaaata agattgaccg tacagaaact gtgtgagctc agaaagagca ataccatgtt
    20641 tgaatacgag tttatactta tgcttctctc ttacttgttc ttttatacaa tattctagac
    20701 actcggctag actttctaga tcgtgaagga tcgttggtgt agtcaccctt agagaaatag
    20761 ctgtttgttc gatgtctatt tcttggaatt caaataattg ttcacacaga tacacttttc
    20821 taaatatatt tcgctcgatg taattttcca gcattcctga tacacgcctc cagtttttct
    20881 tatgatttta tttaatcata tctgtaaatc gtatttttgt ttccttttta tttagaaaag
    20941 ccctttcaaa aaccaataaa aaaagaaact gggacaaaag tcaaacaagc tcacaatttc
    21001 agcctcattg taggtagctt tgagccgttt ggacttttac cccagcctct ttcaagaatg
    21061 gttattcaaa gatgtcagcg tctagttctt tgactacatc tttcaacaat tggataccag
    21121 ctgcgctgtt accaaatgga tctagtgccg ggctgaagac acctatgccg taacgatttg
    21181 gcacagctgc catcaacccg ccgcctacac cgctttttgc aggtacacca acatggacgg
    21241 aaaactctcc tgattcatca tacaaaccag cagttgtcat aattgatttg acgattgtag
    21301 cactttcttc agtaatgatt tgtttgccat tccaaggagc aattccttta tttgccagca
    21361 cagcagctaa ttttgctatg cctgtagcgg tgacattgag ggagcattgt ctgaaataag
    21421 catcgagtat ctccggtacg tctccttcga tcatttgatt acctttcata tagtaagcaa
    21481 gagagcgatt gatatcacct gttcttgatt cagaatgata gatttcttca ttcaatgtga
    21541 tattaggatc atcacagatc tctttcataa atgacaagat tcgctcaaat ttttcatctg
    21601 catctttacc ggcaatcaac gaggttgttg caatcgctcc tgcattaaca aaaggattca
    21661 ttggatgttt gcgatgattg atttccatat tcatgataga attgaaggca aaaccggttg
    21721 gttccgtgtt gattgtttga aagacttttt ctattccgtt gtctttgatt gctaataaca
    21781 atacgggaac ttttgacatg ctttcgatgg caaagcgcac atcggcgtct cctccctcaa
    21841 tcagttgttt tgtatccaaa tcataaatcg ccatacctaa ttgtttggga tccacttcag
    21901 ctaatgctgg aatgtaatcc gccacttgtc ctgtttgata atagtggaaa ttttttccaa
    21961 tcaattgttc taattctttt acttccattt gaataaccag ctttcatttt ttatttttct
    22021 gcatgctgga atttatgacg gcatgcgtag atcgtaaatg gaataactaa gacgataaga
    22081 taagaaacaa tcagaatcgt ttcataaatc ccatgctgtc cagctgcgat actagcaggt
    22141 ggaacaaatg agatacaaaa agcaaaaagt gaagtcaaaa gacctaaaca agcaacgatt
    22201 acttttacaa cttttccgcc tggaacctga taggctcttt caagactgtc atctttaaaa
    22261 accagcttca aatagcccgc gaaaaacata aagtacaaca tcaggtagat tacaacagtc
    22321 aatgacatcg ctgcaagaaa tgaaagatta ttccccccgc cgcttaatgt aaagacggct
    22381 gcccagattg ttgcaacgat cccatttgcg atcatcaaat gagcaggaac accgtgtgtg
    22441 ttttcttttg caaaatattc aggaagtaat ccttcttctg ctgctacgtg aagtgctttt
    22501 gaaggagagg ttacccaaga agcaatttct ccgatgacac ctaaacaaac aagaatcgca
    22561 agaattttcg caatccatgc aagatttgga tcaatgtgtc ttaataggaa ttctaacgat
    22621 tgaatcaccc ctgtattaag agacaaacca tgttgaggta ctgtagtagc gacggtcaat
    22681 ccgccaaaag tatctaaaat gattgccaga ataaccaata caaacattgc taatggataa
    22741 tcctttttag ggttttccat ttcatttgca tgactggcag atgtttctac gcccatatat
    22801 gctaaaataa aggaaacaaa aacaaccagc gtaggcaatt tcgaaaaatc aggaatcaat
    22861 gttttcatag acagtatcgt ttgtagtgga gcgcctgaag cgacataatg gatccctaag
    22921 ccaaacaaga ctaatgctgg caacaagata ccagctacaa aaccgatccg agcaatcaat
    22981 gaagtgtatt tggttccttt taactgtgag agtaaaatag cccagaaaat aatcaatagg
    23041 attgccaatt tcagatattt attgttttgt atagcaccaa tacccgttgc ataggataat
    23101 gcaccagtta agaaatacag catggtcaag tagccaactg ttatctcaaa ccattggaaa
    23161 aagattgcgg caaaaccgaa tcgttttccc aatgtgcgac tgacccaagt atagacaccg
    23221 cctttttccc atcctttcac cgttgccatc tctgctgcgc acaatgccac agggataaac
    23281 catagcaaac cgcctaataa caagaaaaat actaagctga agcctgaggt cgcgaatgtg
    23341 ggatattcat ataatgaaac gaccattgag atggtcatag ccaaaaagcc aaagaacgtt
    23401 aatttttttg caggaatttt cgttttgctt tgttgcatga atcttcctct tttctatttg
    23461 aatttgtttc aattgagttt gttgccaaag aagaaaaagt ccaatccttc ttcttgttct
    23521 tcttttgtag tagtgatcgt tgtaaagtgc atcgccgatt ttaaaatcac tgttgtcagc
    23581 acataggcat acacggcagt aatgatgaca ccaatcactt gaatccctaa gaagtaccag
    23641 ctgcttggat tcgttagtaa atggctgtta gatgcaaaga tccctatcag aatcgtacct
    23701 gtgaatccgc ccatgccatg aacaccccac acatcaagtg catcatccca ttgtttcttt
    23761 ttacgaaaat caacagcagc atgacagacg atacccgcaa tgatcccaat cagcatggca
    23821 ctttttgctt ctacataacc ggcacaaggc gtaatggctg ccaatccagc aacggatcct
    23881 gttagtacgt cgacaaaatc aaagccattt ccttttactt ttgcaatgat cagccaagta
    23941 atcatcgcaa atgcaagagc gatgaatgtt gaaccaaatg cattgactgc ttgttcatct
    24001 gctcgaagtg caccaccgga attaaaaccg aaccagccaa accaaagcag tcctgtaccg
    24061 atagcagctg ccataagact gctatgctga cttctttctc ctaaaggaat ctttctttga
    24121 ccaagtgtga ggatacatgc taaagagccg aatccagctg tcgtgtggat gactgctcca
    24181 ccagcgaaat ctctaaatcc catctgatcc aaaaatccgc caccccatat ccagtggcaa
    24241 acagggaaat aaatcagcag attccaaaaa ataacaagta taatatagcc ttttaaattc
    24301 aatctccctg caaaagctcc tgtcatcaaa ggaacggtaa tcacgataaa catcagttgg
    24361 taaagaaaga acattaaaaa tggaatcgtt gcaccatgaa acatgttggg gaagcttgta
    24421 acatgtctca tcaaaaagaa atctgccggg tttccaataa caccgccgat atcccgtcca
    24481 aaggccaatc cgaatccacc aaatacccac ataatcgttg ttatcccaac ggagagaaaa
    24541 gattgagaca tgatcgttag tatattattc ttccgtgcta atccgccgta aaaaaaagct
    24601 agaccaggag tcatcaggca aaccattgct gtacataaaa tcatgaatgc tgtatctcct
    24661 gtattgatag gtaatgtatt catttttttc gcctcctttc tacgattacg agtataaaag
    24721 aaggtgtagt tgtttttttc ttctgttttt ttgaattaat acttgcattt ataaaagaca
    24781 gacaattaat aaaagcttga tgtgttgtgc tctttttcgt ttttcacaca aataaagaca
    24841 aaaaataaac ggtcttcttt ttatagaaaa ccgtttattt tcgtcttttt ttatttttaa
    24901 agatattttt tcataaggaa taaagaatac ttcatataaa atatataaaa aagaaccgca
    24961 gcgagtgtcg ctacggttct caaatttgtt cttcatttca acataagttt ttcttcaatt
    25021 tgcttataat ttacgtgcaa agaatgaaac gactaatact aaaatgattg caccgatcaa
    25081 tgatggaatg atagccattc ctgcaattgt tggtccccaa gttcctagta aagcttgtcc
    25141 aattgctgaa cctactaaac cagcaatgat gtttgcgatg atccccattg atgatccttt
    25201 attcgtgatt gctcctgcaa ttgctccaat gataccgccg acgattaatg cccaaataaa
    25261 tcccatttta aattcctcct aactgacttc ttacttctta ttgtacacgt ggttcgttat
    25321 tttctttgac tttttgtgca cctttgccta cagcagattt tgctttatct gtttggcgtg
    25381 aagcaaattg tcctgtggat tttgctgctt ctgttacttt gtcttgtact gtttcttgat
    25441 ctttttggta ttcttcttgt gttttgatgt ctgcaacatt cacattcact tcgataacat
    25501 ctaaatgtgt catattgttt acttcagtac tgatcaattc tttgatttgg ttgtaaatat
    25561 cttcgatgtc tttaccgtat tctacgatca catctagatc cacagctact tgtttcttac
    25621 ctacttctgt attgatacct gctgttacgt tatctgtgtt gactaatttt tcagccatat
    25681 ttgagaaaaa tccgccatct aatgtcaata gaccatctac atctttcaat gcataaccga
    25741 tgattttttg gatgactttg tcttcataag tcaattcacc attcatcatg cggttttctc
    25801 ctttttcatt tgccaaaact gatccatttg ttgtattcat gtttttgttt tccatctttg
    25861 tttcctccta tgatttaaaa aattgatcaa ctattcctga ctgtttcaaa taaaaaccga
    25921 ttcctgcgcc aattgctgtc atgattatga tcaacaatgt cttgaaaaat ccaatcgtta
    25981 accataaaac agctagcagt agtccaagac ccccaaagat aatagggagc ttgtatgctg
    26041 aaaatagttc tttcattatt tggccctcct attccactcg agagtgcttg tagtctcttg
    26101 tatcttcttg ttcttcataa ccgctgtatg tgactgccac tttgaccgtt tcttttgttc
    26161 cgagtacttt agttacttct tcttcgatat cttgcatcaa tacacctgtt ttccctacta
    26221 atgaagaagt tcgtttcaag tctccgttga catggatatg gattcgattt ttcgttgaac
    26281 gtatccgtac tttaggtgaa tgaatgaact cttcttcgtg taaatgggaa cgtaccaacc
    26341 cttcgatcgc ttttttgtcg attgttagtt tgccatcttc ccgtttcata acgaaagttc
    26401 ctcttgactt tggatagaaa agaacgacta gcaagatgat aatagcaaga acggcaaata
    26461 ctacagctgc ccagaaaata tattgttgca tgtagtaatt cgttaaagta aagaagcgaa
    26521 atcgttccaa ttgttcaggc ataatatacg gcgcattgat caacgcaatc gctagaaata
    26581 ctgaaagtag cagtaaactc atgataacac cgattatttt taacccctta ttcattttcc
    26641 acctcctata aacttatttt taccctgtaa ataactctac cgacaatcac agtatagata
    26701 gcgaacagtt agcatagcat cagtaaataa agcgctacca aacattgctg gatcataacg
    26761 gcgttttatt gccagtctga tactgaaata acaattgctt acttattctc tatttacttt
    26821 tgattgctgg ctatacttaa aggatattat tcaagattaa aaattgcaaa gattatgctc
    26881 agttattatt acaagattct attt
//
LOCUS       DAYLZO010000023        26660 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.023, whole genome shotgun sequence.
ACCESSION   DAYLZO010000023 DAYLZO010000000
VERSION     DAYLZO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.023"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..294)
                     /locus_tag="ACGVJG_000920"
     CDS             complement(<1..294)
                     /locus_tag="ACGVJG_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HFJ6556896.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAI"
     gene            complement(631..810)
                     /locus_tag="ACGVJG_000921"
     CDS             complement(631..810)
                     /locus_tag="ACGVJG_000921"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFJ6556897.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(857..2680)
                     /locus_tag="ACGVJG_000922"
     CDS             complement(857..2680)
                     /locus_tag="ACGVJG_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFJ6556898.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            2958..5237
                     /gene="helD"
                     /locus_tag="ACGVJG_000923"
     CDS             2958..5237
                     /gene="helD"
                     /locus_tag="ACGVJG_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFJ6556899.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(5330..5977)
                     /locus_tag="ACGVJG_000924"
     CDS             complement(5330..5977)
                     /locus_tag="ACGVJG_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556900.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(6156..6875)
                     /locus_tag="ACGVJG_000925"
     CDS             complement(6156..6875)
                     /locus_tag="ACGVJG_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6556901.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(7006..8157)
                     /locus_tag="ACGVJG_000926"
     CDS             complement(7006..8157)
                     /locus_tag="ACGVJG_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFJ6556902.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(8374..9114)
                     /locus_tag="ACGVJG_000927"
     CDS             complement(8374..9114)
                     /locus_tag="ACGVJG_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFJ6556903.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(9118..9441)
                     /gene="rsfS"
                     /locus_tag="ACGVJG_000928"
     CDS             complement(9118..9441)
                     /gene="rsfS"
                     /locus_tag="ACGVJG_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFJ6556904.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(9498..10091)
                     /gene="yqeK"
                     /locus_tag="ACGVJG_000929"
     CDS             complement(9498..10091)
                     /gene="yqeK"
                     /locus_tag="ACGVJG_000929"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFJ6556905.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(10075..10725)
                     /locus_tag="ACGVJG_000930"
     CDS             complement(10075..10725)
                     /locus_tag="ACGVJG_000930"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFJ6556906.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(10735..11046)
                     /gene="yhbY"
                     /locus_tag="ACGVJG_000931"
     CDS             complement(10735..11046)
                     /gene="yhbY"
                     /locus_tag="ACGVJG_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFJ6556907.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(11062..12171)
                     /gene="yqeH"
                     /locus_tag="ACGVJG_000932"
     CDS             complement(11062..12171)
                     /gene="yqeH"
                     /locus_tag="ACGVJG_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFJ6556908.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(12168..12695)
                     /locus_tag="ACGVJG_000933"
     CDS             complement(12168..12695)
                     /locus_tag="ACGVJG_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFJ6556909.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(12875..13660)
                     /locus_tag="ACGVJG_000934"
     CDS             complement(12875..13660)
                     /locus_tag="ACGVJG_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFJ6556910.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(13653..14522)
                     /gene="accD"
                     /locus_tag="ACGVJG_000935"
     CDS             complement(13653..14522)
                     /gene="accD"
                     /locus_tag="ACGVJG_000935"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFJ6556911.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(14523..15902)
                     /locus_tag="ACGVJG_000936"
     CDS             complement(14523..15902)
                     /locus_tag="ACGVJG_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFJ6556912.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(15910..16329)
                     /gene="fabZ"
                     /locus_tag="ACGVJG_000937"
     CDS             complement(15910..16329)
                     /gene="fabZ"
                     /locus_tag="ACGVJG_000937"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFJ6556913.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(16354..16830)
                     /gene="accB"
                     /locus_tag="ACGVJG_000938"
     CDS             complement(16354..16830)
                     /gene="accB"
                     /locus_tag="ACGVJG_000938"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFJ6556914.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(16833..18068)
                     /gene="fabF"
                     /locus_tag="ACGVJG_000939"
     CDS             complement(16833..18068)
                     /gene="fabF"
                     /locus_tag="ACGVJG_000939"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFJ6556915.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(18087..18824)
                     /gene="fabG"
                     /locus_tag="ACGVJG_000940"
     CDS             complement(18087..18824)
                     /gene="fabG"
                     /locus_tag="ACGVJG_000940"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFJ6556916.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(18836..19753)
                     /gene="fabD"
                     /locus_tag="ACGVJG_000941"
     CDS             complement(18836..19753)
                     /gene="fabD"
                     /locus_tag="ACGVJG_000941"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFJ6556917.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(19860..20084)
                     /locus_tag="ACGVJG_000942"
     CDS             complement(19860..20084)
                     /locus_tag="ACGVJG_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFJ6556918.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(20137..21099)
                     /locus_tag="ACGVJG_000943"
     CDS             complement(20137..21099)
                     /locus_tag="ACGVJG_000943"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFJ6556919.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(21109..21549)
                     /locus_tag="ACGVJG_000944"
     CDS             complement(21109..21549)
                     /locus_tag="ACGVJG_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFJ6556920.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            21855..22820
                     /locus_tag="ACGVJG_000945"
     CDS             21855..22820
                     /locus_tag="ACGVJG_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFJ6556921.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(22877..23107)
                     /locus_tag="ACGVJG_000946"
     CDS             complement(22877..23107)
                     /locus_tag="ACGVJG_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFJ6556922.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(23193..23678)
                     /locus_tag="ACGVJG_000947"
     CDS             complement(23193..23678)
                     /locus_tag="ACGVJG_000947"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFJ6556923.1"
                     /translation="MVGGEEAVYDQVLPLFKEFGTTFTLHGSAGKGQHTKMANQIMIA
                     GTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNYSPRILKEDYTPGFFVKHF
                     IKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDGTQALIKLWWPEGKIPEKK
                     S"
     gene            complement(23661..24079)
                     /locus_tag="ACGVJG_000948"
                     /pseudo
     CDS             complement(23661..24079)
                     /locus_tag="ACGVJG_000948"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(24168..25391)
                     /locus_tag="ACGVJG_000949"
     CDS             complement(24168..25391)
                     /locus_tag="ACGVJG_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFJ6556924.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(25408..26454)
                     /locus_tag="ACGVJG_000950"
     CDS             complement(25408..26454)
                     /locus_tag="ACGVJG_000950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFJ6556925.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         7408 a         5854 c         4591 g         8807 t
ORIGIN      
        1 gatagctggt ccaacgccaa agtggatatt tggatagagg cgacgcatgg cttgggccat
       61 caaatgagct gctgaatgac ggactaagcc aagtgcatct tcatggtctg gtgtgatgat
      121 ctcaatactt gcatcttcct cgattggacg agtcaaatca acgagttttc cgttcacttt
      181 accagctaaa gctttttttg ctaagctttt tgaaatgctt tcagcaattg ccaatgttgt
      241 tgttccagac tcaaattctt tgactgcacc atctggaaaa gtaattttta tcataatagt
      301 ttcctccttg aaatgttttg attgatcaga tcatcaagct gaaaaacaaa aaaatcctag
      361 catctcttga aaagagatac taggacgaaa agttcgtggt tccacctaat gttcaaacgc
      421 aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt gcttcaacaa gggtttcgaa
      481 agtggtcatc ctttggtttt ctcctggaaa agtttcagcc tagcttttcc tctcttatcg
      541 aagactctca aattcagttg gctttctcat caatcaatta tgtgtttatt taaccactaa
      601 taaatagaaa aatcaagtgg gaaactaaac ttattttttc cattccccat ttacaccgta
      661 tgttcctttt ttcatctcat tgatgatgac atggatgtgt tcttttggtg cctgcgtgtt
      721 tttatgaaca gcttccgtga tatctttcat catattttct aattgttccg gactgcgacc
      781 ttctactaat tcgacatgta cgaatggcat aatgctctct cctctttctg atcgttcttt
      841 tttcaactcg attctatcat actatgtttg tcttgggtac tgaatcagga aaaatggctg
      901 gatctttttt tgctctcaag gcactcaaaa tagagaccgt atccacgact tcttgcaaaa
      961 cagctccgaa taatgcgggg attagtcccg tacttgcaat taacatcaaa aaaatacaaa
     1021 taaagatgcc aatcagtaca gactgtttcg ctattctcat cgtatctctt gaaatcacta
     1081 ctgcttttgc tacacgcaag agatcatctt tcaagatgac cgcatctgca ctttcactag
     1141 cagctgtaga accatgagcc cccattgcaa taccgatatc tgcagcagca agtgctggtg
     1201 cgtcattcac gccgtctcct accatcgcaa caggtctttg atctttaggg atactttcta
     1261 aaatggtcag tttatcttga ggcaagctac gggcatgtac agaatctaat tggacttttt
     1321 ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat catcatcaaa tttttcacat
     1381 caagttttct tagctccttg attgtctttg ctgcttctgg gcgaatctga tccgaaaata
     1441 ggatcgttcc tgcaaaagat cgatcaattg ttacatagat tgctgtattg gacgtttcta
     1501 tttcttcagc tgaaacaaaa gaggcacgtc caatctttac ttcatgatta ttgactactg
     1561 cttggattcc ttgaccggtc acttctttca gttgactaac aggatacagt tggctgtttt
     1621 tcacgttgtt cactaacgaa cgagctaaga tatggctaga ttcctgttct gcactagctg
     1681 catacagaag tagctcttct ttttttattc cattgactgg ctgaatcgcc tctacttcca
     1741 atatcccttt ggtaagcgtt cctgttttat caaaagcaat cgtttttgta cgtgcgagct
     1801 tttcaatcgt tgttccagtt tttacaacga tcccgttttt acttgaacgg ctcattcctg
     1861 caactaaagc aatgggcgca gctagaatca gtggacaagg tgatgccact accagtacct
     1921 cagcaaatcg taaagggtct tttgagaacc accaagaaat accaccgatc aaataagcaa
     1981 tcaacgtgaa agggactgca taacgatcag ctaaacggac aaatcgggca ggcttttctt
     2041 ttgattcttc gactagttta ataatcattt gatactggct gtctgcggca cgtttttcag
     2101 cgatcatttg gatcgatccg tctccattta aagaccctga cataatctga tctccaggtt
     2161 tcttggtttc cgggcgggac tcacctgtca aagaagattc atcgaccgaa gattctcctt
     2221 tggtcactcg accatctgcg ggtacaagtt cgccaggctt gactagtaac tggtcatcaa
     2281 tatttatttc atcgattact atatcctgca attttccttc tttgaatcga tgggcaattc
     2341 ttggggtgtt atctaacaaa gactgcagtt ctttacccgc tcgtttattc gcgtaatcct
     2401 ctagactatc tccgcccgta agcatgatca atatcatcaa actagcccag tattctttga
     2461 cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc tacgccatat cttcctgaac
     2521 gtaacgtctt gatcatttcc aaaaacatca agaaggcgag aatccctcca gtaaatgcga
     2581 caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc acttaataaa gctatcccac
     2641 caataagcaa agtaagtaaa aattttttga aatgtgtcat tgttttttct cctcttttat
     2701 gtgacttaga gaggttagta ataaatttcc agctgcctct ttcatcttaa gcatagtcct
     2761 tgactagcac tttttaaaac aataggcctt aattgaaaac aactatcact atcagcgttt
     2821 ccaaaattca tcagttctac aaacaaaaat attcagcaag gattactttt tcgattcatc
     2881 agttaaaaag tacctttttt cgaaaaacta tgctatactt ttaattcgtt atttacggcg
     2941 gaaggagcaa acatgcgatg aatgagaaac aactagaaca aacgcacgtg gatgaaacga
     3001 tcgaactaat ccagatcgaa caaaatatgc tggatacaaa acaacaagaa ttgactcagg
     3061 aaatgaatca ttcgacaaaa gacacagcta atcacaagat tcgagctggt tccaatgaag
     3121 ctttttatga atctgctgtt gaatatcgcc aacatgagca ggaattgctt ttgaaatacc
     3181 atactcttga agcacagcaa aaacgcctgc aaacgttgtc tgtcatgaaa gatagtcctt
     3241 attttgcccg tatcgacttt aaagaagacc gtgaaacaga aacattatat ctaggtatcg
     3301 cttctttaag agatctggca gaagaaacaa tcgttattga ctggcgcgca ccaattgcca
     3361 atctttatta tgaaggtgag attggtcccg cattttatga aactgaaaca gatagaatag
     3421 atgtcgatct gcagttgaaa cgacaattta aaatccaaga aggacgtatt ttatctatgg
     3481 tagatacgtc ggaagtaatc aatgatgact ttttgctaga gatattggat gaatcatcaa
     3541 gcaaccatat gaaaaacatc gtctctacga tacaaaaagc acaaaatgca atcattcggg
     3601 acacaacaag taaagtaatg ctgattgaag gaatcgccgg aagtggaaag acatccgctt
     3661 tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa gtggctcgag gctgaaaacg
     3721 ttcttctatt ttcacccaac catttgtttt ctgattacat ctcgactgta ttgccttcat
     3781 taggtgaaag cggcgtgcct acacagacat tcaaaaatta tttggtgcag ctgctgccag
     3841 agttttcttt actagatgaa cagcaacaag aaagtggttt tttgctaggg gaaaaagatc
     3901 cgatacaagt gatgaaaagc ggattgacct tagtcgatca gattgatcgc tatatccaag
     3961 gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat aaatggccgt acgatcctat
     4021 caaaggaatc gattcgccaa tggtataaag aaacaaatga acaattacct ttacatcata
     4081 gactttctct attacaaacg aagctcctga aaaaattagg cggtctgcaa aaagacgaaa
     4141 cacgtcagca atgggtcaaa gatctggcgg aagaacagct ccaagaacta tacgcaacag
     4201 atccaaatct tgaatataca gaacaaaagg agcgttcatt acgtaaaaaa ctggccaacc
     4261 aaatcgtacg taaaagattc cgtcgaatcc atcgagggat catgaattat caatttgtca
     4321 atataacgaa acaatatctc cactttctac aaactgtgcc actttctctt ctaacacgac
     4381 ataaaatcag cgagaccagg tggcaggaac atttgacaac tgttcgtaca cacttgcgtc
     4441 aaagagaatt gcgtcaagag gatgctgtac tattcttttt actgatgcgc cgaacatatc
     4501 cagtgacggt tacacacaaa gctcgctata tctttattga tgaaatgcag gatttcgcac
     4561 catcacaagt ggcattgctg caagccttgt tcccaaaagc aaatttgact ttctgtggag
     4621 atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg atcattggaa gaccttttcg
     4681 tggaccgtga agtcactcgt ttccaattaa cgacaagcta tcgctccaca aaagaaatca
     4741 ctgattttgc taatcatttt attgtgaaag aacatcaagt agaaacaaca gcaagaaaag
     4801 gagaccttcc tttgctcatc caaggaacaa cagatgatat caaattgaat tggttagcag
     4861 ataccattcg aacgactcag ggaaaagccc ggtattggcg tacagcaatc atagctaaaa
     4921 cacaagaaga atgcgaagct ctttatgctc aactgccaga cgagttgaaa caacaagtcc
     4981 agctgatttt aaatgagagt gactttatga agcgttccat tattttactt ccagcctttt
     5041 tagcaaaagg tctggagttt gaccgagttt tcttgtggga tgtagatgca gaacattttt
     5101 cttctcagca agacaaattg atcctttaca ccatgtgtac acgtgcgatg catgaattga
     5161 ccctttttac ctccaataat gaaagtcctt tgttaaacaa tatggatatt tcatcttatg
     5221 agaaaaaagt actgtaagta aaaagacaac tgctatcatt aatgttagca gttgtctttt
     5281 tatttacaat aataattgca gaaaaagtaa ttcaatcatt taaggtcatt tattctatat
     5341 ataaatccaa atctgtttca ggatatttta atttgacaag cgaaataatc attttcagtt
     5401 cttccatgtt ttctggatat tctgggtcaa ataccttgct atgacggcca atcccgccat
     5461 tgtcgatcaa tcggtaaatt cgattccatt caatgaattg gtcaattgat tggaaattgc
     5521 tgattggata gccgccacaa tccaaaatct ctgatacacg aaatgctagg tatgcactat
     5581 cgatcacttt attttttttc gtgtctatct tctcatttct ttcgataaga attttttcag
     5641 gacgatgttt caagctccac tctaatagta gaaagaaagc ctccatccga tactcttctt
     5701 caaataaccg aaaggctgaa tcagaacgac gaaaaagcag cggaatcaga taattttgtc
     5761 tataaatcag cattggtgcg cggtccacca tacactctaa ataacggttc agataccctg
     5821 taccgacata gtttcctaat cgatagcctt caacgccgaa tcttgcgtat ttttgcgcta
     5881 attttgaaca atcagttacg ttcacagcta actcttctac tttgcggtaa ccgcttaaga
     5941 tacgttcaaa aggttctaaa gtgatttttg cttccatatt cttccccgtc catcttccac
     6001 atacttcata tcatttcaca tgatacttat gctctcatca taccacaggt tgaattagaa
     6061 aagaaaataa atcacacaaa aaagcaagac aatctgttag tgagattgtc ttgcttttcg
     6121 aaaaagactg aatacttttc gttaaaactg gttgcttagt cttctaatgc cacattatgg
     6181 aacacatgct gtacatcttc tagatcttcc agtgcatcaa tcattttttc aaatgtttct
     6241 tgatcttctg gagatagtgt tacctcgttt tgagggatca tttggatctc agcgattgaa
     6301 aaatcagtga ttcctttttc ttttaatgct tcttgtatac tatggaaatc cgctacttca
     6361 ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc gaacatcgat gtctttttcc
     6421 attaggtatt caaggatctc gtcagcgtct tctcctgcaa aagcaaagat tgctgtattg
     6481 tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc cgccattttt cccaaatgct
     6541 gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca atgtatcaac aatcaccatt
     6601 gagccgcttg gtccaaatcc ttcatagcgc aattcagagt atgtttcatc tcctgcacct
     6661 tttgcttttt cgattgctcg gtcaataata tgttttggta cattataagt tttagcgcga
     6721 tcaatgacga aacgtagttt ttggtttgca tgcggatcag gatcacctga ttttgctgcc
     6781 gcgtagattt caatcccaaa ttttgcataa atccggctat tattagcatc tttagcagtt
     6841 ttctttgcta cgatatttgc ccatttacgt cccatatttt cacttcgctt tctgatttga
     6901 tagtttcata acgcaaaaac gtcctgttcc attatacttg tgaatcacga ttctgtgaag
     6961 ctttttttct ttcttccttc attcttctca tttttagtca attatctata ctcggattgg
     7021 gatttgcccg aaattctgtt ccggaatatt gcctcccaat tgatagattt gatcacttct
     7081 aactaacagt tccgcttctt gtgcctcttt ttttccgatt cgggaataaa tagaaatctc
     7141 ttttttttct tttaagaatc ttcttccagc atcggtaaat ccaagtagtc gaagataatt
     7201 tttattttgt tggacaggaa tctcttgctt tttgatattc agcaagacat aacaagataa
     7261 tcgttggatc cgtgtccatg tatatctttt tgtttttacc tttgacacaa aatcctcaaa
     7321 actatcagca gttgcagctg ctgttttcat gcgcgattct attccttcgg tcatctgata
     7381 gatatctgtc agttccagca aagtagaaga taacaaccga taacgcaaat acggccaata
     7441 agattcccaa gtctgcagcg aacaagttga aagatcttct tctgtctgag gcggcacaag
     7501 tccagctatt ctttttcctt cttctaacgc atggcgaatg gctgttgcac tggctatcct
     7561 ctgatctgtg atgatttttt catgatagcc tgcactttta cgtcggatcg gatgtaattc
     7621 catagggtgg acatacttgg cattttcctt tgcataactt aatcccaaga tatgatttgg
     7681 cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga aaaacctcag tcattttttg
     7741 cggataacta tacgtttgat cagacagttc ttgaaatgcc tcttctatcc tatcttgatt
     7801 ctcttggaca aagcgtccga aactgctgta atcaaacgaa gtctcttctt ctgttccaaa
     7861 acacaaactg tcacagtcca gtgtttgcaa gatttttact gcacctttag caaaatagtc
     7921 agctgactga accgcccatt cgacgggaag ctccactact aaatccacac cattttgtaa
     7981 agcttcattc gcgcgatgcc atttatcgat gatagccggt tcgcctcttt gcaaaaaatt
     8041 cccgctcatt acagcaatga cgacctctgc tcctgttatt tttcgtgcca tttccacatg
     8101 atagcgatgt ccattgtgaa aaggattgta ctctacgata atcccgcagc ttttcatgcg
     8161 catcctcctt catagttatc gataaaaaag aaatagagac aagtaaaaaa aggcaaaaat
     8221 cggaaaaaat acccgaacta tataaactat tctcgcaaac acaatacttc tgcgacgaaa
     8281 agctgcactg caagagcaat gcctatatcg ttcggatata taatttatgt tgaagattta
     8341 ttcttttctt gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta caaagaacca
     8401 gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt cgactttcga
     8461 aaagccgctg ttttccagca ttcttaaata actttccaat ggataggtac gctcttgatg
     8521 tagttcatct tcacgaataa atcgaccaga cccttcttgt tcttcaacga aaaaggtaag
     8581 aaaatgttcg atgctgtgtt tctcgcttcc tggataacta tcccacaaaa aagcaaaatt
     8641 atccccttga taatgatagc tatattctgg aaaaacctca tctacttgat aggtggagtg
     8701 tacgtcaaat aaaaatcggc cattttcttc taacgcttga tagacttcat caaacacttg
     8761 ctgtacctct tgtctatttt tcatatagca cagcgaatca gaaaaacagg taattgcctg
     8821 atattgtccg atttctgata aatcgagcat attgccttcc acaaattgga tcgatgcgtc
     8881 ttcttcatat gctcgctggc tggcaatcat caacatctct tctgaaaggt ctaaccctgt
     8941 cactttatat ccatcttttg caaaggcaac cgctaatgct ccagtaccgc atgccagttc
     9001 caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa aaagctaacc attgctgata
     9061 aagtgtttcg tccatcactt catcgtaaat gaaagcaaat gtttcataag ccatatgtta
     9121 atccaaccat tcactaagat cgactagcgg agcatctgac catagttttt ccagattata
     9181 gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca tctccaagat cgatcaatac
     9241 ccattttccg ccttcttttc cttcaatacg ttttacttca tatttgtttt cttcttcttt
     9301 atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg aacagatcat
     9361 gaaataatct gccaacaagg aaacatttcg aacgtctaaa gcgataatgt cttctgcccg
     9421 ttttgaatct gctgcttcaa tcgcaatttt taattgttct ttactatcta tgatgatttc
     9481 ctcctaatat gagcagctta tttttttgct acccagtaat tatatgtttc gattgttttt
     9541 ggataaatct gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt ttcaaatgct
     9601 acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc aggaaaatct
     9661 cgacctggtt caatatagtc tgctacataa atgattttgt caaggagact catcttagca
     9721 gccccagtcg tatgcacccg gattgcatga agaatctctg catcggtgat tcctaattcc
     9781 cgctctacaa acgaagcgcc aacaagacca tgccagattg cattattata attcaataac
     9841 tccggatcaa aaccgtctct tacgattacc attttgaact cctcatctgg tcgttctttt
     9901 gcataatcat gtgtcaatgc agcaatacta gctttttcag gagaagcacc gtattttttg
     9961 gcaagagcca cggctgtttc ttctactcca agcacatgct tgaagcgttg ttcgctcatt
    10021 ctcatctgta ttttttgcat caggttttct ctagaataag cagtatattc attactatag
    10081 tccatattca tacaaccctt cattttgaat ataatcaatt actttatctg gaacaaggta
    10141 gcggatagaa cagccttctt tgatcttttg acgtattttc gtggaactga tgtcgatctc
    10201 aggtacatct acccaaatta caggatatgg agtatccgtt gtgtatcctg gtcgacgtat
    10261 acccacaaag ttgaccatag aagtcagctc atcgatttta taccattttg gcaaatattc
    10321 taccatatcc ccaccgataa taaagtaata atctgtatcc ggattgtttt gcgtcaattc
    10381 cttcatagta tcataggtgt aacttttccc accacgagct aattcaatcg tttcaatctg
    10441 caaaaaagga ttgtcttcca ccgctaattc caacatgttg agtcgatgtt ttgcatcaat
    10501 cgtctgtttc tcatcaacat gaggtggctg ataagaaggc atcaaaaata cgcggtcaag
    10561 acctaaattc cggccagctt gttcagccat cactaaatgt gcaaggtgaa cgggattgaa
    10621 tgttccgccc aagatcccta cttgcttttt cttctctaag aattgaggca tttcttcagg
    10681 aaataattgt gaacgaacaa atgtcttagc ttgcgtattc atcatggtca ctccttatat
    10741 tgcttttact tcttttgata gcctttgata cttttctttt gtggaaggtt tgtataatac
    10801 aataacacgt ccaatgattt ggacgatttc gcaatctatt tctttttcta atacttgagc
    10861 tgcttcttct gcaatctcat ctgtattttg aagtaacgat actttgatca gttctctttt
    10921 ttctaaagct tcttcaattt gaacgatcat tgctgaattg atcccacctt ttccgatttg
    10981 aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt ttcctcttaa
    11041 actcataatt ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat ctacaccctt
    11101 tggcgcccag cccgcaatta ctactggcgc tgttacagtg atccagccta gaccggcaaa
    11161 aacgatgtct gttttttctt tcacagaaaa ctcaaaacga acaagctccg gaaatgcctc
    11221 gacttcatca ggacgaggag gctgtaataa tccacctaca tgtttttgat aaaattcgtc
    11281 tgctttttcc agtttcgttc gatgaatatt tacatcattt gacacataag caataaatga
    11341 agcacgattg ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa gtgtttgccc
    11401 ttcattcagc tgataaactt taggtttgat ttcttttaac ggtgcaatga tccgcaaatc
    11461 ttttttacct aagtagtgcg ccatttgatg gcgatggata attcccggtg tatcaatcaa
    11521 aaaatgcccg tcatctaaag gaatctcgat tttatccaag gtcgttcctg gaaactgcga
    11581 agttgtgatt acatcttgta ctcccgctgt atttttgatg atttgattga taagcgtcga
    11641 ttttccgaca tttgttactc cgacgatata gacatcccgt ccatcgcggt attcttcgat
    11701 tttttctaat agttccgtca tctcatgaga tttcttagca ctagttaata atacatctac
    11761 tggacgaatt cctgcttcgt aagcacgttc cttcatccat tgtgttaatt tagttttttt
    11821 caatgattta ggtaatatat ccactttgtt cccaactaaa agtactggat tgtcaccgac
    11881 aaaacggtgt agtcctggga ttagtgaacc attgaagtca aaaatatcca ccacattgac
    11941 gatcaatgca tctttttgcc ctaattcatt cagtaagcgc aaaaagtcgt catccgtcaa
    12001 tgagacatct tgggtctcat tataatggcg taatctgaaa caacgctgac aatagacttc
    12061 cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt caccgggttt
    12121 gtttgtttgt ataacggcac cacagccgat acattgtaat tcttctgtca ttcgattcca
    12181 cctcgccagc ccatatcagg gtgttttgct aatagatagg ccataattct tttttcccaa
    12241 aaacgattaa attgtgtctt ccaactatct gttgcgataa tgggttttac aaggatgcta
    12301 cgtatccctg cacgattggc tccacgtata tcagtcataa tctgatcgcc taccattact
    12361 atctcatcat ctgctaaatc caattgtttc tttgctatgt tgatccctct tgctaaaggt
    12421 ttcattgctc gtgcaacata caaaagatca aatttttcaa tcgctcgcgc gacacgtttt
    12481 gaattattgt ttgaaacaac aacaacaggg attcctgcat ttttcatttc taaaatccaa
    12541 gtaagcaact cttctgttcc atctggatta ttccatgcaa tcaacgtatt gtctaaatcg
    12601 gtcaatactg ctttgatgcc tagattcttt aattgggcag gtgtaatctg atagatggct
    12661 tctaccatcc atgttggttt gtaatttgaa aacatgctgt ctcctcatct gcaaaaaatg
    12721 aaggagcaca taaagtactc ctcaaccccg tattatactg catatcagca aaaaaaacta
    12781 cctctatact cgttcgtctt tcagaaaaag catttcttta caaaatgacc aaaaataaag
    12841 attgatcaca gaaaaattta gaaacaaaaa acaactaata ttggcggaac ctcttgtatc
    12901 gtttttccag taatttatgc tgatctaatc gaacaagttc cgtcaatctt tcaatcaacg
    12961 cttttttcat aatgtgtgcg actttatatc gtggcaactg ctcgccgttg aacgtttcag
    13021 gaataactcg atcaacgatt cctaactcgc gtagttctgg cgcagtaatt ttcatcaact
    13081 cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat ccttctggag
    13141 aaagcacagc ataaatcgtt tcttccagca tccagacttc atccgctaaa cctagagcaa
    13201 gggcaccgcc acttccacct tcaccaatga tgatggcgat gatcggtacc tttaaatcag
    13261 acatttcgag cagatttctg gcaatagcct ctccttcgcc gcgctcttct gctcctacac
    13321 cgcaatatgc acctgctgta ttgatcaagg taatcaccgg tctattgaac ttttccgctt
    13381 gtttcatcag tcgtaaagct tttcgatagc cttcaggatg gggagaacca aaatttctgc
    13441 ggatattttc ctgcagatcc cgtccttttt gaataccgat cactgttacc ggtttgccat
    13501 ctaatcgagc gattccacca acaatcgctt cgtcatctgc aaataaacga tcaccatggc
    13561 attcctcgaa agaatcgaag atctcttcaa aaaaatccaa gcttgtccat cgttctttag
    13621 ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac gatctatcca tcctttctgt
    13681 gtatgcaatt caagaagcgt atggattttc tgtttcaatt ccatgcgggg aacaatctga
    13741 tcgacaaaac catgagataa taagaattca gccttttgga aatcttctgg aagttcctgc
    13801 ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg gggttctgct
    13861 agaataatat cgccttccat tgcaaaactg gcagttactc ctccggtcgt cgggtccgtt
    13921 aaaacagtta gataaaaaag accttcattg ctatgccgtt tgacagcagc agagattttt
    13981 gccatctgca ttagagaaaa gattccttct tgcatccgtg cgccacctga agctgtgaac
    14041 aagacgactg gaagactttc ctttgttgct cgttcgaata atctggtgat cttttctccg
    14101 acaatcgttc ccatacttcc catgataaaa ttaggatcca tcactccaat aatgaaggga
    14161 atcccctgaa tggttgcttt accagtgagg actgcttcat gaagtcctgt tttatcttgc
    14221 atttttttaa ttttttccgt atattcggga aatgctagcg gatctttggt tacaagatcc
    14281 gtatcccatt cttcaaaact tttttcatca ataatcaaag ctagacgctg ccatgcaccg
    14341 attcgaaaag cgtatccgca atgagggcat actttttctt caccgatgtc tttcgtataa
    14401 aggatatgtt tgcaatttgg acactttgcc cacatgttat caggaacaga tggcgagtta
    14461 gcaggtttgc tctctctatt aggattgatc cgaatatatt tctttttctt aaacagagcc
    14521 atctatgcgc ctcctatctc cgtttctggt gtccaattcg gtaaaaattc ttcttgtaaa
    14581 aatgctgtgc tgtaatcacc tgctatgact gcaggatgac taatcaaatc catctgaaat
    14641 tctgcgttcg tgatgattcc ttctgtgata aattcactta aggctcgctg catcttcatt
    14701 aatgcttcaa aacgcgtatt tccatggacg attatttttg caatcattga atcataatat
    14761 ggcgggatcg tatatcccgg gtaaactgca ctgtctactc gcagtcccat cccacctgct
    14821 ggaagcagta agttttggat cttccctgga gaaggagcaa aatggaaagc aggattttct
    14881 gcattaatcc ggcattcgat tgcgtgtcct tggaatacta cttcttcttg acacacattc
    14941 aaaggctcac cggctgctat ctccacctgt ttttttacta aatcgattcc tgtgaccatt
    15001 tctgttactg gatgttccac ttggattcga gtattcattt ccatgaaata aaaatcgcct
    15061 tcttcatcca tcagaaactc gatcgttccg gcattttcat aattgactgc tttagctgct
    15121 cgaacagcag cgtctcctag tgcttctctt tttgtttgtg agatcactac agaaggagat
    15181 tcttccagta ctttttgatt gttgcgttgt aaagaacaat ctcgctcgcc taaatgaatg
    15241 acatgaccaa atcgatcacc taagatctga acttctatat ggcgagcagg ataaatgatt
    15301 ttttccaaat acatatcatc atttccaaat gccgctgctg cttcttgctg agctgaagaa
    15361 aaatgctgtg ggagctcttc ttttgattga actttccgaa tgcctttccc gccaccacct
    15421 gcagctgctt tcagcatcac tggataacca attctgtctg ctatttccaa tgcttcttct
    15481 actgtagata atacaccagt acttcctgga atgactggga catttgcttc ttgcatcaat
    15541 tgacgcgcat tgatcttatt tcccattgca tcgatcgttg cacttttagg accaataaat
    15601 gtgatattgc actcttcaca catggaggca aatcggctat tttcagataa aaaaccaaat
    15661 cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc ctgaacattc
    15721 agatatgaat caacggcttt tgccgggcca atgcagattg cttcatctgc taactgtgta
    15781 tgaagtgctt cggcatctgc ttctgaatat atcgctacgg tttggatccc tagttcgcgg
    15841 catgcacgaa tgatacgtac agcgatttca ccgcggttag ctatcaatac tttagaaaac
    15901 atatttcacc tatccaatca taaaggttaa ttccgcttcg gctacttttt taccgtctac
    15961 tttagcgatt cctttgccta ttccggctga agctttcact ttcaatatct caacttcaag
    16021 gtataaggta tctcccggtg taactttctt acggaatttt gctttgtcca atccgccgaa
    16081 ataagccgtc tttcccttga attgttcaag agaaagtaat gcaactgctc ctgcttgagc
    16141 cattgcttca acaatcaata cccctggcat aacaggttct tctggaaaat gtccttgaaa
    16201 aaagggctca ttgatcgtta catttttctt agcaacgatc cgttctcctt caaccatttc
    16261 ttccactcga tcaatcaaaa gcattggata acgatgtgga atgatttctt ttatttcttg
    16321 tatgttcatt gttttccctc tttcctgctt tgcttatttt tgctgaatcc taaagagtgg
    16381 ctgtccatat tcgacaactg actcgttttc tacaaggatt tctgtgatca ctccgtcggc
    16441 ctcactagtt atctcattca tcagtttcat cgcttcaata atgcagataa cttctccttt
    16501 ggcaacttga tcacctactt ttttgaaagc tggtttgtcg ggcgctggtt gcagataaac
    16561 gattcccacg attggcgaaa caacctcgat tccatcaaat actgccgatt ctttttcttt
    16621 cattatttct ggagcagttg ttgcttgttc aggtatgaca ttgactggct ccgaaactgg
    16681 caacgtttcg tttgtttgaa ccatattcat gcctcgactt gttttatttt tattcatata
    16741 caattcaaat tgaccttcac gcaaatcgaa ttccgttagc gaggattgat caaattgact
    16801 gactaattct ctgatttcat tgatatccaa aattattcct cccagcgttt catgcacaga
    16861 accgcattat gtccgccaaa ccctaatgaa ttactcattg cataagtaaa acttgcaggt
    16921 ttgctttcgt ttttgacaac tgtcaagtca atctcaggat cttgtttttc tacattgatc
    16981 gtaggtggga taaattgatg ttctaatgcc agcaaagttg ccaaggcttc gatcccgccg
    17041 gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt atctctgaaa
    17101 ttttcaccta atgcgatttg gattgctttt gcttctgcct ggtcattggc tggtgtactt
    17161 gttccgtgag cattgatata tccgacttgt tccggctgta caccagcttc ttccatcgca
    17221 agcttcattg ctttagatgc accagaacca tctggagtag gagctgtcat atggtaagcg
    17281 tcacagtttg caccgtatcc aacgatttct ccatagattt tggcaccgcg agcttgtgca
    17341 tgttctaaag attcaagaac tagaacgcct gctccttcac ccatcacaaa accattccga
    17401 ttttcgtcaa acggaatcga accacgatta ggatcagttg attctgagag tgctgttaaa
    17461 gctgcaaatc cagcaatccc catctcacaa atcgttgctt ctgttcctcc agcaagaatg
    17521 acatcagaat agccatgttt gatgttacgg aatgcttccc caattgaatt cgttccgcta
    17581 gcacaagctg ttacgatagc tgtacaaata ccttttgcac ctacacgcaa agcaatattt
    17641 ccagctgcca tgttggcaat cgccattggt acgaacattg gagaaactcg ttttgcacct
    17701 ttttcatgta gacgaataac ttggttttcg attgttggaa gaccaccgat cccagatccg
    17761 atgatgacgc caaaccggtc ttgattcatt ttttctttat ctaaaccgct catctccatt
    17821 gcttctaaag cagcgtagat tccatagatc gaaaacatat ccattcgttt gctatctttt
    17881 ttaataaaat atttatcgta agggaaatct ttgacttcac ccgctaaggt aatccctgtt
    17941 tctgaagcat caaatttagt gatcggtgcg atgccattcg tcccctcttt taagctattc
    18001 aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc catagccagt aattaccact
    18061 cgattcattt gtttgctctc cttttcttat ccgtgcatga ccaatccgcc atcgacattg
    18121 atgacttgtc cggtgatata tggattttgt gctaagaaaa ctgctgtttg tgcaatatct
    18181 tctacttgtc cgaaacgctg catcggaatt tgcttttccg cttgttcctt gactttgtct
    18241 gctaaaactt ccgtcatatc agttgtgatg aatcctggcg caatggcatt acatgtaatg
    18301 cctctagttg cagcttctcg tgccacagat tttgttaatc ctactacgcc agctttacta
    18361 gctgcataat ttgcttgacc aatatttcca atcaacccag atacactaga gagattaatg
    18421 atggcccctt ctcgctgctt catcattttt ttcaatacgt gctgcgtcat attgaaggtt
    18481 ccaatcaaat tgatatccag acattttttg aaatcttctg catccattcg catcacaagt
    18541 ttgtcatttg tgattccggc attgtttact aacacatgga tcgatccaag cttttcttct
    18601 gcttctttga tcatttgtcc cgctttctca taatcagaga tgtcaccgga aactcctacg
    18661 cattttacac cgaatgcttc gatttcttct atcttttctt ttggaatctc tccgcgacca
    18721 ttcaaaatga tatttgctcc agcttttgca aaagctaagg ccatcgcttt gccgattcct
    18781 cttgtactac ctgtgataaa tacatttttt ccttttacat ccataacgtt cctccttata
    18841 gtattgctat tgtttcatca aatgtctgct tattctcaac acgcagaact ggtattgttt
    18901 tatcgatttt tttcataaat ccgcttaata cttttccggg gccgacttcg atgacttgtt
    18961 cgatgccgat tgtttttaac ttatggatac tctcatagaa acgaacaggt tgcatgactt
    19021 gctgttcaag tagtcccgcg attgtttctt tcttcattat ttccgttgtt gtgttgctta
    19081 tgataggaaa agaaggttca gagaattgaa tctgacttag gtccttcgcc agtttttttg
    19141 ctgccggttc taaaatagct gtatgaaaag gcccgctgac atttagaggg atcattcttt
    19201 tgaacccttt ttcttttaat agtaaaacag cttcgtctac tgctttttct tcgccaccaa
    19261 tcacgatctg ctgaggtgta ttataatttg caggagatac gatgccgtac ttgctagctt
    19321 catgacagct ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc attttcccgc
    19381 ttccagctgg ggcagcttct gtcatataag cgcctctttt tgctactaac gcaactgctt
    19441 cagtgaagga tagcgcatta ctagctacta atgccgaata ttctcctaaa ctcagtccta
    19501 aagctgcatc tgggatgatt ccatgctcct tcaataagcg ataatacgcg atgctgactg
    19561 ttagaatagc tggctgtgta tattgcgtct gatccaaacg ttcattttct gtaaagcaaa
    19621 gttcagccat atcgtagccg agaatctcac ttgcttcatc aaatgtctgc tttacaacgg
    19681 cttcttcata taaatctttt cccatacctt gatattgtgc accttggcca ctaaataaga
    19741 atgctgtttt catgtttttc tccaatatat ccagaatata tgtttgattc ttcctattca
    19801 aaaaggccga gacattccta tttcaggaaa tgtcccagcc cccaacctaa ttttaatgat
    19861 tatgcagttt gtttttctac gtaagtcact aaatcttgaa ccgttttgat tccgtcttct
    19921 gtttcgattt tcacgtcaaa ttcatcttca atttcattga tgatttggaa taaatccaag
    19981 ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc agtttctttt
    20041 cctaattctt ctgcgatgat ttcttgaatt ttttcgaata ccataataat atattcctcc
    20101 gatattgttt gctttttatt tttataattt aacagtctac aactccatga gaatacttcc
    20161 ccatgttaaa cctccgccat agcctgtaaa tattactttc tgctgttttc ctaaggtgat
    20221 ctttccacta gaaactgctt catccaataa gatagggatg cttgcagcag atgtattccc
    20281 atatttatcc atattggtca ggaacttttc ttgcggcatc tttacttttc gggcaatttt
    20341 ctcaataatt ctgaggtttg cctgatgcaa aagcagataa tcaactggtt tgtctttcat
    20401 gacttcttgg atattgtttg caacgtctcg tacggcaaaa tcaaaaatat ctctgccagt
    20461 catttgcaaa taatacgaac cttctgaatc ttctgaatag aacggatttt tattttgctg
    20521 gtagccagcg gtcaatgagc tacttctggt accgtcagct gctaattttt ctttcagcaa
    20581 ttttttctgt ggattcgctt ctaagagaat cccacctgct ccatcgccaa acaacacagc
    20641 agtcgtacga tcagaccaat cgattagctt agatagtgtt tcaccaccaa tcaccagtcc
    20701 cttagttgag ccgcagcgga tcattttttc agccatgctt aacgcataaa caaatcctga
    20761 gcacgcagca ctgatatcaa aagcgatagc atgagttgct cccaatttcc cttgtacttg
    20821 tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt gctactaaaa taaaatccaa
    20881 atctttaggg tttagatttt tctgttttat caatttttcc gccactaaaa tgcataaatc
    20941 agaagtctcc tgatttgtta cacatcttcg ttcacgaata cccgtccttg acgaaatcca
    21001 ttcatcagag gtatccatca tcttggaaag atcattatta gtaataatct tttcaggaag
    21061 gacgctggct gttgcagtaa tcattccaaa ttgttccatt aaatatcctc acttatagtc
    21121 ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt cctccgtagc
    21181 cattccgtct aagatgttct tgaccatttc acgatgaaaa tgctgatgga cccgaaatag
    21241 taactttcct tttttcgtta gtcccaactt tacaacgcgg cggtcatctt cactgcggat
    21301 acgttcgaca tagccttttt ttactaactt gttaatagca gtggtcaagg tcccaacagt
    21361 aattgatagt tctttggcta cttctgaagt tgtttttttc ttgtacatcc cgattgcctc
    21421 gatcgtgtgc atctctgtga ttgacagatc tttgaattga gattttttca gctcagactc
    21481 ttcgattgtc aaaatatcat tgaagacact aacaagataa tcatttactg tttttaaatc
    21541 tggttccatg ttccaccgtc cactaattag tttgattatc aaatcttttg atgttcaaac
    21601 tatatcttaa cttttttcaa aatgcaacct taaaagatca tttttttact atatgagctt
    21661 tttttaagat tttactcatt taaaacaact gataaatcag catttataat ttcttttatt
    21721 agaaaaatca tctatctcac atattttgat tatcaaagta ttttctgtga acagaactat
    21781 agaattccct aaaacttgtc cattatttca aaatttgtta tagtcttttt attcaaagcg
    21841 taaaaggaga tcctatgtta aaagaaaaaa tgaaaactct ccccatgctg ccgggtgtct
    21901 atcttatgaa agaccgagat ggtaaggtca tctatgttgg taaagcaaaa cggctcaaaa
    21961 atagagtaag cagctatttt catcagaata aacagcattc aaaaaaagta ttacgtatga
    22021 ttcatcatat aacagatttt gatttcgtcg tagtagacac ggaattagat gctttattgc
    22081 tcgagtgtca attgatccaa cattaccgtc ctttttataa ccgacaaatg aattatttca
    22141 gtaattataa ttatgtacat attaccaata agggatttgt attaacagat actcctacgg
    22201 ctcgaacata cgggcctttt cgtctctata aaaaaatgcc gtctattttg cgtataatgg
    22261 aagaaactta tcaaatgcct tggttgtctg aaattagttt gttagctttg cgagtccagc
    22321 ttcctgacct acaggaaatg acttttgaac agaaaaaaaa ggaactccaa gggctgtttc
    22381 aaggccgaaa taaaaagcta ctgacttact tgaaaaaaag acaacaacat ttcatctatc
    22441 agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat cgaattagtt acttatttta
    22501 ttagacggat ccaggaacaa aagcagtttt taagaacacc atcgctcacc ttcagcatgc
    22561 cattagcagc agatgagagt cagaaaaagc actacctcgt ttgttatggt caacttgcag
    22621 aaacaatgat tacctctggt gacgtttctc ctgacttcta ttatgaaaag aaagaagctc
    22681 atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga tcctgtacaa atcttgatca
    22741 gttaccgcaa aaaattagaa aaagaacagt ttgaaattga actgttaaac aaaaaagaag
    22801 ctgaaaagca actaaattag ttgcttctcg gcttctttta ggcatcttca acttcaaatg
    22861 tcaattatcc taaaatttat tggattaagt catagatttc catcgctacg atatcgatat
    22921 tatcaaattc gtattgctga tgagtatctg tttcagttaa ttcaaatgta tcggtcgctt
    22981 tatcataagt tactgtacac ttctctacgc cttctttttc aaaacggcga acttgcagtt
    23041 cattatctgc tgactcgacc atagcctcca gtcgttttat gattgcgact aattgtgaat
    23101 gtgtcatcgg aggtcctcct tttcttattt ctgttatatt gtatcaaaag catagcctac
    23161 ttgcattgat tttctctaaa aaaactcgtt ttttatgatt ttttttctgg tatttttcct
    23221 tcaggccacc aaagtttgat caaagcttgg gttccgtcat tctcaaaacc tttatctgcc
    23281 aactcttcat atagtttcaa tgcttgggta gttgctggca atacaagatc cattttttct
    23341 gcttcttcta atgcaatctt taaatctttg ataaaatgtt tgacaaaaaa accaggtgtg
    23401 taatcttctt ttaaaatacg aggagaataa ttgcttaaag accagttagc tgcacttcca
    23461 cctgaaagcg tctcaatcac tttctttaaa tcaagtccgt ttttttgtgc atagacaagc
    23521 atttccgtca ttcctgtcat cgtacctgca atcatgatct gattagccat ttttgtatgc
    23581 tgacctttcc cagcagaacc atgcaatgta aatgtcgtac caaattcttt gaataaaggc
    23641 agtacttgat cataaacggc ttcttctcca ccgaccatga tcgtcagtgt accattcttt
    23701 gctcctaagt caccaccgga taccggtgca tctaacgcat ctgcgccttt ttcttttgct
    23761 gtttttgcaa ttttttctgc caggctggtg tacttgtagt caaatcaact aaaatatgtc
    23821 ctgagatatc tgcagaaaaa atccccgact gtccatagta gatttcttct acatctttgg
    23881 ggtaacctac cattgtaaag agaatattac tttgttctgc taccgctttt ggtgtatctg
    23941 cccagatagc tccttctgtt actaaaggat cagcttttgc ttttgtccga ttatagacag
    24001 ctacttcgtg tcctgcttct agtagatggc gtgcgacagc acttcccatg acgcctgttc
    24061 cgataaaacc aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc
    24121 tagtatacgc tagaaacaag gattttttca taaaaaatgt ttgaatttta tcttttaaat
    24181 gaatttaatt ttattttgat tcgttcaatc gagtcttctt cttcttttct tcgcatctct
    24241 tcttcatgat aaacaaccat gtcttgataa aattcgatca tagcttcgcc gaaatgtgta
    24301 gatgaaatat cataaagttt agcttctaaa acttttggat ctttaggaac ttcactggaa
    24361 atataatcaa taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa
    24421 ggatgatcga acaatcggtt caaataagca tttccttcca cgacacaagg tgttccggct
    24481 gccatcgctt ctgtataagt aagcccttgt gtttctgaag tagaagcact gacaaaataa
    24541 tctgccgctt tataataaag tgctacatca ttgttgtcca cttctcctgt gaactgcaca
    24601 aattcttcta actgcagttc tgagatcaat tcctctaact cttctacata aggtcctttt
    24661 cctacgataa ctaatctggc gttaggataa gcggagatga cctgaggaaa cccattaatc
    24721 aaggcttgga tatttttctc ataagaaatc ctgcttaaag aaagaatcat caaatggttt
    24781 tcctgaagac ctagtgactc ccttaatttg ctgatatcct gctgagtgat atcagaacgt
    24841 ttgaattttt caatgtcgat accagtagga atcacgcgaa gcggtgctat tacaccatag
    24901 ctgcgtaatg tatcaataac acgttcactt ggacaaacta cacctgtcgt atgatttgca
    24961 aataatctag acaagtattt tacatgggat tgcctgatca cttttccttt accgatataa
    25021 tgaaggtagt cttcatacat cgtatggtaa gtatggatca gaggtatttt caacttctta
    25081 gctaccatct tcccaagtat ccctgctccg aattctgtat gggtatgaat aagatctagt
    25141 tctagttctt tggcaaccaa ataagcatac cacatgcctc taacaacaac tcttcgatcc
    25201 ttgaaagaaa taaatggtac acttggcatt cgaatcacat ctttttccaa cttatccgca
    25261 ttaggatcgg tcgttgtaaa aatatagact tcatgcccat gtctttctaa ttcttgtttt
    25321 aatgttttga tcgatgtagc aactccgctg acttgcggaa agtaggtatc ggtaaaaaag
    25381 ccgattttca tgtttttcgc ctccaaacta gtccttgacc tgatttactt tttttaattt
    25441 gctagagact ttttcattca gtacactttc gtatacattt ttaagttctt caccgatttt
    25501 attgatactt cgctcttttg ctgtttgata accggcttgt gtcgtactcg gtagttcttg
    25561 attggtaatc tgttggatca gtcggataaa ttcttcgttt gtcttcccca tatagcagtt
    25621 cttgctatct tctaaccagc cattatacac tggaatatct cgaacaagta cgttttgttt
    25681 gcttgctaaa gcttctaaaa caacgatccc ttctgtctct tcataagaag ggaaaaagaa
    25741 tagatctgca cctgaatacg caccttctat gatctctcca cgaatgtaac caggaaacag
    25801 gacattttca ggatgatctt cttttacaat tttccggatg ttccgaggaa tcgagtacat
    25861 gggaacatga ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa
    25921 atcaatgatt cctttacgtt cgaaaaacaa accgacacaa atgattactt tttgatccgg
    25981 agacagtttg aaatattctc tgaacttttg ttctttcatt ggatcagggt gatatttctc
    26041 taagtcgatc ccattggaaa tagcttgaat cggcacacgg ataccatagc tttccaacaa
    26101 ctgttttgaa taaggtgttg gcgtgatcag atagtccgct ttttggtaca atcctaccag
    26161 atattttttt acgataggag aaagctggtt cgatctgata aaggaatttc ggaaatcttc
    26221 ctctgtagaa tgcgcatgat agacaacttt ttttccttct cttctggctt tattgaccat
    26281 attatgacta ttgattccgt atgtattgat gtgcaggatg tcataatctt ctttttcatc
    26341 tagtgtataa gagataccga cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc
    26401 gattccagat tttgcaagca tcttttcact ttcaaaatat aataatacct tcaccttacg
    26461 tcccccttca cttatatagt ataacatagc ctacaaactg tcactcaaac ttaagcccta
    26521 tttttgtcta ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta
    26581 gctcttttac aagccacacc agcttttgat acgttgttta ttttacatat tcatgaacaa
    26641 gttcaacgac ttcttccatc
//
LOCUS       DAYLZO010000024        26093 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.024, whole genome shotgun sequence.
ACCESSION   DAYLZO010000024 DAYLZO010000000
VERSION     DAYLZO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.024"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(46..1185)
                     /locus_tag="ACGVJG_000951"
     CDS             complement(46..1185)
                     /locus_tag="ACGVJG_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFJ6556926.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1584..2882)
                     /gene="eno"
                     /locus_tag="ACGVJG_000952"
     CDS             complement(1584..2882)
                     /gene="eno"
                     /locus_tag="ACGVJG_000952"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFJ6556927.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2998..3753)
                     /gene="tpiA"
                     /locus_tag="ACGVJG_000953"
     CDS             complement(2998..3753)
                     /gene="tpiA"
                     /locus_tag="ACGVJG_000953"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFJ6556928.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3857..5047)
                     /gene="pgk"
                     /locus_tag="ACGVJG_000954"
     CDS             complement(3857..5047)
                     /gene="pgk"
                     /locus_tag="ACGVJG_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFJ6556929.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5157..6158)
                     /gene="gap"
                     /locus_tag="ACGVJG_000955"
     CDS             complement(5157..6158)
                     /gene="gap"
                     /locus_tag="ACGVJG_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFJ6556930.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6199..7290)
                     /locus_tag="ACGVJG_000956"
     CDS             complement(6199..7290)
                     /locus_tag="ACGVJG_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFJ6556931.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7439..8773)
                     /gene="rpoN"
                     /locus_tag="ACGVJG_000957"
     CDS             complement(7439..8773)
                     /gene="rpoN"
                     /locus_tag="ACGVJG_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFJ6556932.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8833..8904
                     /locus_tag="ACGVJG_000958"
     tRNA            8833..8904
                     /locus_tag="ACGVJG_000958"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8866..8868,aa:Arg,seq:ccg)
     gene            complement(9096..9980)
                     /locus_tag="ACGVJG_000959"
     CDS             complement(9096..9980)
                     /locus_tag="ACGVJG_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFJ6556933.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(10067..10966)
                     /locus_tag="ACGVJG_000960"
     CDS             complement(10067..10966)
                     /locus_tag="ACGVJG_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFJ6556934.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(10983..11489)
                     /locus_tag="ACGVJG_000961"
     CDS             complement(10983..11489)
                     /locus_tag="ACGVJG_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFJ6556935.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(11678..12298)
                     /gene="rpoE"
                     /locus_tag="ACGVJG_000962"
     CDS             complement(11678..12298)
                     /gene="rpoE"
                     /locus_tag="ACGVJG_000962"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFJ6556936.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(12374..12811)
                     /locus_tag="ACGVJG_000963"
     CDS             complement(12374..12811)
                     /locus_tag="ACGVJG_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFJ6556937.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            12974..13804
                     /locus_tag="ACGVJG_000964"
     CDS             12974..13804
                     /locus_tag="ACGVJG_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFJ6556938.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            13788..15155
                     /locus_tag="ACGVJG_000965"
     CDS             13788..15155
                     /locus_tag="ACGVJG_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFJ6556939.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            15174..15983
                     /gene="yidA"
                     /locus_tag="ACGVJG_000966"
     CDS             15174..15983
                     /gene="yidA"
                     /locus_tag="ACGVJG_000966"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFJ6556940.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16026..16469)
                     /locus_tag="ACGVJG_000967"
     CDS             complement(16026..16469)
                     /locus_tag="ACGVJG_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFJ6556941.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            16603..16974
                     /locus_tag="ACGVJG_000968"
     CDS             16603..16974
                     /locus_tag="ACGVJG_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFJ6556942.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(17143..17925)
                     /locus_tag="ACGVJG_000969"
     CDS             complement(17143..17925)
                     /locus_tag="ACGVJG_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFJ6556943.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(17922..18893)
                     /locus_tag="ACGVJG_000970"
     CDS             complement(17922..18893)
                     /locus_tag="ACGVJG_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFJ6556944.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            18985..20379
                     /locus_tag="ACGVJG_000971"
     CDS             18985..20379
                     /locus_tag="ACGVJG_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFJ6556945.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            complement(20571..21926)
                     /locus_tag="ACGVJG_000972"
     CDS             complement(20571..21926)
                     /locus_tag="ACGVJG_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFJ6556946.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(21956..22591)
                     /locus_tag="ACGVJG_000973"
     CDS             complement(21956..22591)
                     /locus_tag="ACGVJG_000973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFJ6556947.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            complement(22598..23974)
                     /locus_tag="ACGVJG_000974"
     CDS             complement(22598..23974)
                     /locus_tag="ACGVJG_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFJ6556948.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            complement(23967..25748)
                     /locus_tag="ACGVJG_000975"
     CDS             complement(23967..25748)
                     /locus_tag="ACGVJG_000975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFJ6556949.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            complement(25770..26081)
                     /locus_tag="ACGVJG_000976"
     CDS             complement(25770..26081)
                     /locus_tag="ACGVJG_000976"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFJ6556950.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
BASE COUNT         7309 a         5560 c         4639 g         8585 t
ORIGIN      
        1 aaataatgct aatctggctc tgttttttca tttattaaaa aaatcttacg ctaagtagga
       61 taaaataatt ccttgatatt tttcgatcat atccaaataa ttatcgattt caacatactc
      121 atctacttga tgaggcaaag taacgacacc aggcccaaag acgacaaagt cgaaggaatg
      181 gttagctttc gtaaattcgg ctgcatccgt tgttgctgcc gctccaacta aaggtaatgg
      241 ctggctgaat tgttgctgaa tgcaatgaat caaaggagaa tctggatcag ctttgacagg
      301 tattttgttg taatcgatca tcaattccaa atgatagtct gtctcttgat tcaattcatt
      361 gacgattgat tgcagcaatg caatgatttt atcatttggg tattctggaa tcgatcgaat
      421 gttcccttgg agctgcgcat ggcttgggat gctgttgact tgattcccgc cactgatcaa
      481 tgtaacatta tggattgttt ttcctaggac aggattctcg attgtttcag cgaggtgatt
      541 catttcagca tttgctttcg taatgaattc atttaggtga ttgattgcat tataaccttg
      601 gtctggcata gagctatgcg cttcttttcc gtgcgaggtg actgtatagt tgattgatcc
      661 catgtgcgta tacatcaaac tgtaattggt tggctctccg atgatcaagg catctaagtc
      721 atctacatag cctgctttcg tgagctgttc cccacctagt tcacccactt cttctccgac
      781 agtggctaat aatttgactg ttccgttaaa tggttttcct gactctttca gttcgatcat
      841 ggcaatcacc attgctgcca agccactctt catatctgta gcaccacgtc cgtacaaacg
      901 gttcccatgg atttctgctg caaagggtgc ataggtccaa gacgattcat caccagccgc
      961 cacgacatcc atatgcccag ataggcctaa cacttttcct gattggccct tttggtaacg
     1021 agcaatcaaa ttatctctac catctctgta agaaacaatc tctcctgtta tgtcatgctt
     1081 tgccaaaagc ttattcaaat aggctgctac ttcaccttcg tttccattca ctgatttaat
     1141 tcggatgatc tcttgtaaaa tcgcgatttt ttcttctttt ttcatctaat cctctccctt
     1201 gccatttctt tttattgtat ttgagtgtgc tcgaattttc aaataggagc aaccaaagta
     1261 aaaatgaaga aagagagcca ctttatgaaa aatatgctgc tacttccctc ctattttatt
     1321 tatacgagag gacttgctca tagattccta ttctactgtt ctatccttaa aagctcttat
     1381 atgatgctag ttattgttag ctttcctttt tcaaggatga tcttttcttg taccattctt
     1441 tgtaaacatc atatacagaa gctagttgca aaaaaactct ctacctgctg atatttcagc
     1501 aagtagagag ttcgattact taactaagtg atcatactcg actatctttc aatagtcgat
     1561 catgtatctt cattagaaaa gaattatttg ttttttaagt tgtagaaaga tttcaaacct
     1621 ttgtattctg caacttcacc aagttggtct tcgatacgta gtaattggtt gtatttagca
     1681 atacggtcag tacgtgatag agaaccagtt ttgatttggc cagcgtttgt tgcaacagcg
     1741 atatcagaga ttgttgaatc ttctgtttca ccagaacggt gagatacaac tgctgtgtag
     1801 ccagcttctt tagccatttc gatagcttcg aaagtttctg ttaatgtacc gatttggttc
     1861 actttgataa ggattgagtt agcaattcct ttttcgatac cttcagataa tttttgagtg
     1921 tttgttacga acaagtcgtc accaactaat tgaactttgt cgcctaatac gtcagttaat
     1981 ttcttgaatc cgtcccagtc gttttcgtct aatccgtctt cgattgagat gattggatat
     2041 ttagaaacta attcttcgta gaatttgatc atttcgtcag ttgttttttc gccttcgcct
     2101 gaatcagcta gtacgtaaac acctttttct ttgtcgtaga attctgaaga agcagcatcc
     2161 atagcaagaa caacgtcttt accaggtaca tagccagctt tttcgattgc ttcgatgatt
     2221 acttcaaaac cttcttcgtt tgaaccaagg tttggagcaa atccaccttc gtcaccaaca
     2281 gaagttgcta atccgcgagc tttcaagatt gaagctaatg cgtggaatac ttcagcaccc
     2341 atacgtagag cttctttgaa tgttggagcg ccaacaggca tgatcatgaa ttcttggaag
     2401 tcgattgagt tatcagcatg tgatccgccg ttgatgatgt tcatcattgg tgttggcaat
     2461 acttttgtgt tgaatccgcc taggtagtgg tataaaggta cttctaggta gtcagcagct
     2521 gcacgtgcaa cagcgataga aacacctaaa atagcgttcg cacctaattt acctttgtta
     2581 ggagttccgt ctaaagcgat catagctttg tcgatagcca tttggtcacg aacatcgtag
     2641 ccaatgattg cttcagcaat gatgttgttt acgttgtcga cagctttagt tacaccttta
     2701 ccgccataac gagctttgtc tccgtcacgt aattcaactg cttcgtattc accagttgaa
     2761 gcacctgaag gaaccattcc gcggccaaaa gctccgcttt ctgtgtatac ttctacttcg
     2821 attgttgggt taccacgtga gtctaagatc tcgcgagcgt aaacatcagt aataattgac
     2881 atgttttgtc tctcctttga gtttgttttt tcctaaggga tttccctcat atacgatttt
     2941 agttaatttt gctcattagt gcaaacattt cttgcatttt tcgcaaattt tcaacaatta
     3001 ttttacagcg tctaacaatg acaagaatga atctgcttgc aagcttgcgc caccgactaa
     3061 agcaccgtca acattttctt ttgccatgta ttcagcaata ttttctggtt ttacagaacc
     3121 gccgtattgg atacgaactg cttctgatac ttcttttcca taaagttttt caactgtttg
     3181 acgtacaact ccacaaattt cgtcagcgat ttgtgcgtca gctgatttac cagtaccgat
     3241 tgcccagatt ggttcataag caataaccat gtttgacacc tgttcagctg aaagatcagc
     3301 taaaccagct gtaatttgtc cttcgatcca ttcagcagtt ttgcctgctt cataggtttc
     3361 taaagattcg ccacagcata ggattggtgt cataccattt gcaaagatag ctttcgcttt
     3421 tttattgatg tcttcgtctg tttcatggaa atattcacga cgttctgaat gtccgatgat
     3481 cacataatga acacctagat cagcaagagc tgctggtgaa gtttcaccag tgaacgcacc
     3541 tgagttttcc cagtagcagt tttgtgctga gatttttagt tctgtacctt cagctgatgt
     3601 cattaattct tgtaagaata atgcaggaga tccgatgact gaatcaactt tgtcatttga
     3661 agggattttg ttttttactg cttcagcaaa ttcttttgct tctgatgcag ttttgttcat
     3721 tttccagtta cctgcgataa taggtttacg cattgaggaa cacactcctt aatttattga
     3781 aaaatggcta gggagtcatg aaaactccct tcaccttttg ttcgattact cgtcagaatt
     3841 gagcaaactt ggtcgtttat ttatcgttga ttgctgctag tcctggtaat tctttacctt
     3901 caagcaattc caagcttgcg ccgccacctg ttgagatgtg tgtgaatttg tcagcaaatc
     3961 ctaattgttc tgctgctgcg gctgagtctc cgccaccgat gattgttgtt gcgccttcta
     4021 agttagcgat tgcttcacaa acaccgattg tacctttagc aaagttactc atttcaaata
     4081 cacccatagg tccgttccaa acaactgttt ttgcaccttg taatgtatca gcaaataatt
     4141 ttactgatgc tgggccgatg tctaaagcca tcaaaccatc aggaatgtct ccttcgatta
     4201 cttgtgtttc aacatcattt gaaaattctg gtgcacagac attgtcgatt ggcaagacaa
     4261 gtttgtcgcc tgctttttcg attaattctt ttgctagttc cactttatca gcttctacta
     4321 gagagttacc gatcttgatt ccttttgctt cgtagaatgt gtaagtcatt ccgccaccga
     4381 tcaagatttt atctgcttta ggaatcaagt tttcgattac gccgatttta tcagaaactt
     4441 ttgcgccacc aaggattgca atcattggtc gttttggttc ttctactgct tcaccgatga
     4501 acttgatttc tttttccatc aagaatccag caactgttgg gataccagtt gaagcgatac
     4561 ctacgttaga agcatgtgca cggtgagctg taccgaaagc atcgttaaca aatacatcgc
     4621 ctaatgaagc ccagtattta cctagttctg tatcattttt gctttctttt ttaccgtcaa
     4681 tatcttcaaa tcgtgtattt tcgaatacga caacgtcgcc gtcattcatg ttgcggattg
     4741 cttcttctaa ttgttctccg cgagtttcag gtacgaatgt tacttcttta cctaataatt
     4801 cgcctagacg ttttgcaact ggagctaaag attttccttc tttatcttct tctgttttta
     4861 cacgtccaag gtgagagaaa aggattgctt ttccgccttg ttctagaacg tagttgattg
     4921 ttggcaatgc tgctttgata cgagtgtcgt cagtgatcac gccgtctttc aaaggaacgt
     4981 tgaagtcaac acggacaaga acttttttgt cttttaaatc gatatctttc actgttttct
     5041 tagccatttt atttcctcct gaataaactt attatcaaaa aaagcggaag aagcgcgatt
     5101 gcttccccgc ttttaaatgt acatgtctat aatttacact tgtacgtttg tctatcttat
     5161 aagttagcga agtattctaa agtacgtact aattgtgcag tgtatgacat ttcgttgtcg
     5221 taccaagcaa cagttttaac taattgtttg tcgccaactg tcattacttt agtttgtgta
     5281 gcatcaaata atgaaccgta agtcatacca acgatatctg aagaaacgat ttcgtctgtg
     5341 ttgtatccgt aagattcgtt tgaagcttct ttcattgctg cattgatttc gtcaacagtt
     5401 acttcttttt caagaactgt tactaattct gttaatgaac ctgttggtac aggaacacgt
     5461 tgagcagctc cgtctaattt accgttcaat tcaggaatta ctaaaccgat agctttagca
     5521 gcacctgttg agttaggaac gatgtttgca gcagcagcgc gtgcacggcg gaagtcacct
     5581 ttaggatgag gtccgtctag agtcatttgg tcacctgtgt aagcgtggat agttgtcatt
     5641 aatccttcaa caacaccaaa tttgtcgttc aatgttttag ccataggagc taaacagtta
     5701 gtagtacatg aagcacctga gataactgtt tctttaccag ttaatgtttc gtggtttgtg
     5761 ttataaacga ttgttggaac atcgtttccg ccaggagcag agataacaac gcgtttagca
     5821 ccagctgtta aatgtttttc agctgctgat ttagaagtga agaaaccagt acattctaga
     5881 acgatatcta cgcctagttc gccccatggt aattcttctg ggttgcggtt agctaatact
     5941 ttaacttctt ttccgtttac gttgaatgaa ccttcatgaa cttcaactgt tccgttgaaa
     6001 cgtccttgag ttgtgtcgta ttttaacaag tgtgccaaca ttttagcatc tgtcaagtcg
     6061 ttgattgcta ctacttcgat tccatctaca tcttggatac gacggaatgc tagacgtcca
     6121 atacgtccaa aaccattaat acctacttta actgtcattt aagatttcct ccttatgaaa
     6181 atcaaaaaaa tatttatttt aaagggttac cccttttaaa atctcatttg cagcagcttc
     6241 atcagtgatg agccacgttt gttttggtgc atttttcata taagctcgaa ttgctttagc
     6301 ttttgatttt ccgcccgcga ttgccatgat gatcgggact ttttccaaat cttgcaattc
     6361 tatgccgatt ctagggacct tataaacgac ttcacctttt tcattgaaaa agtagccaaa
     6421 agattccgca acagcatttg cttgtttcag catgactaat tctttttcag tcatcttacg
     6481 acgtgctgcc atgtgtaatg cacgcccaat actatgaata acacaattcg cttcactaat
     6541 caagtttaag acttcttgga tagatggctc attgagtaaa gaattgtaag ttgccagact
     6601 taattgttcc ggaacataaa gtgcacggtg gttaccgttt gctttagcag ccattcttgc
     6661 actgacagaa tttgcctgta cagttatcgc ttcaccaatc ccgcctcttg cagggacaaa
     6721 caggttatgc cgtttcttat tttccagatt agacaattgt tcggctacca cagccatagt
     6781 agttcctccc atcacagcga tgatattctc gccttctgga agtagtcggt ctagggcttc
     6841 gctcagcgct tcgccgaatg catcagccac tttggtctgc tcttcgctgt ttcctgaaac
     6901 aacgatacag cgttggttac caaaatattc tgccaactgt tgttcggttt gatgcgtacc
     6961 taaaagctga tccatgaagt tttcaaggct ttgataaact tcttcgccct tagttgtcag
     7021 cgtcataccg ctttttgagg tgtcgatcaa attcagattt ttcaaaagat ctgtttctgt
     7081 tcgcaatact cgttctgtga tccccatact ttccgataaa cttcgacgtc caatcggctg
     7141 catccaatag atatttcgta aaattttata gcgttcttgc aacacttcga taatatctgg
     7201 tgcaacagat tcaatcattt ttatttcatc cagcatagcg atctccttag ttggacactt
     7261 tacgtccaat gtagacttaa aacgacccat atacgataaa aaaagaaatt aactattcga
     7321 atttcacatc aataaaggga aatctcaacc tcacgactgt aagtctaaca gttttgctct
     7381 cgtttttcaa ctaaaaagac tagaaaaagt gaaatacttt ttctagtctt ttgactgatt
     7441 agttatcata acgttttctt tttgatgaac tcgggattcc taataaatca cgatattttg
     7501 ctactgttcg tcttgagatc gctatctcat cttttttcag caattcaacc aatttctggt
     7561 cagacaacgg cttgtttttg tcttcttgat cgactaattc ctgcagcttt ttcttcgctg
     7621 tatctgctga aaggtcttct gtctgttctg tactattcgt aggaattcgt gtagtgaaaa
     7681 atttttttaa ttcaaataca ccgaagtctg tttccaaata tttcccgttg actgcacgac
     7741 tgaccgtgga ttcatgcaca tctatctcag ttgcaacttc ctttaacgta agaggcttga
     7801 ttggacgttc cttgtcgata aagaaagctt tttgccggga aacaatgact tgagcaaccc
     7861 gcaaaattgt atccccacgt tgtaggatcg ttttcttcaa ccattcgaat tcctgtttcc
     7921 gttcttttaa ataattttca gtttctttat ctgcttgttg tgacatttgt ttgaagtaac
     7981 tctcttgaaa gcgtatattt ggctgatttt ttcggtttga gataacttgt acatttttat
     8041 cgtcgtccac taatacccgg acatcaggaa taatgtacaa ttcgctgcta cggtcgaaaa
     8101 tccggccagg gcttggactc aacgtttgta aatagtcgaa tatctgctga acagcatgta
     8161 gatcaacaga aaaacgttgt gcgatttctt tccattttcg ctccaccagt tcctcaaaac
     8221 attcttccaa cacgatataa gctaattccg gcgcataatc atctcgttct gtttgaagca
     8281 tgagacattc ttgcaaacta cgtgcaccta ctccagctgg ttctaattgc tgaatcagag
     8341 tcaacgcatc gagcatttgg ataggcgtag ctccaatctc ttttccggct tcttctaaac
     8401 taatcgtcaa aaatccgttc aaatcgatgt attctacaag ataaagaacg attttcctca
     8461 aaaaagtatc tcggtaattc aaatggactt gttcgatcaa tgaatcaaat aaagaccctt
     8521 ttgtatcagg aatctgattt aggtaatttg tctcttctcc ttcataagaa gatgaagaag
     8581 cttttatgta atctggctgc cattccggct caacaacttc caccaatgga ttttccattg
     8641 cttgattctc aacgaatgcc aacagctcct cactgttata ttgtaaaact tggatggact
     8701 gttgcaactg ttgcgtcatg gctagtttct gtgtctgctg ctgtttttgt gaaaaatttt
     8761 gctcaaattt catttttttg tccaatcccc ttgtaatttt ttatctgttt agttacaatt
     8821 atcccagtgc ttgcgtcctt agtgtagtgg atatcacgta agattccggt tcttgagacg
     8881 ggggttcgat tccctcagga cgcgttctac tgctatttta gcataattta cgaatagttg
     8941 cttctttatt tttaagtgaa aactgtccgc caaagaaaat tggtggacag ttttttgttg
     9001 tacctgtata gatattgtta agcctttgca agtaaggtag ttatgtgttc aactacttat
     9061 taaaatgtgt tatttattaa agatacttgt ctcacttact gaactggccg atgatcgttt
     9121 ctgccataat agttctcgac agccgttgga tgccctaata aatccccaac tgtcgtgatg
     9181 cgataccctt gctctttcag ataatcgatc acttgaggaa cggcacgaat cgtttcagga
     9241 tagatatcat ggaataaaat aattgcccca tcgtatactg ttgcttggat cttctgcaaa
     9301 atcaaatcag gatttcttgt cttccaatct tctgaatcaa cagaccattc gatgatcggt
     9361 cgctggatga tcgttgctcc tacattcgtg acagaaccat agggcggacg aacaaaagtg
     9421 ggggttttgc cagtgacttt tttgatctca gtgtctgctt tcgtcacttc ctccgtcatc
     9481 gactgggcag aaagagtggc cagattctga tgatcgtatg tgtgattgcc aatctcgtgc
     9541 cctgcttcag ctgtctgctt cacgatttca ggatgctgcg ccgtattttc tcctaagacg
     9601 aaaaaagtgg catgcacgtt tttttctttt aagattcgca gaagctgcgg tgttgtggtc
     9661 gctcctggtc catcatcaaa gctgattgcg acgcttttga cagccggatc tcggctcgtt
     9721 tttgtactgt cttgtgttga ttgtgatgac tgactttgta ccttttccgc tgattcttga
     9781 atggcttctt tgacgttagc tgtttcagtt gattggcttt taatggttga ttgttctgat
     9841 tgtttgaaag attgtgacga atgctccgaa gagtgtgttt gtccttttac ttcggtgatc
     9901 aaggaggcca ccgaagtaat ttcgaaaaac aaaagcacgg ataaagcaat aatactgata
     9961 actaaataat ttcttttcac tatatctctt ctttctcttt ttctcttctt attatttact
    10021 tgtacttact tgaaaattga aactgctagt cgcaactact tctcgtctaa tgatccaaat
    10081 aatcttccga tgccaaaatc gtttttgtca cggacttagg aatgacaaat tctggtgtac
    10141 ccatataacc aggagcaatt tcgtattgag aaaaagtcaa aactaattga tgctctttgt
    10201 tcaaataaaa acttcgtgta ggcgtaacaa gtgttggttt ttctatcgtc ccttcttgaa
    10261 tatcttgctc agtccaatag tacttagatg gatcttcctt gacttgttcg gccatctgcc
    10321 gtttgacttc ttttgtcaaa acagatagat aggtgtcatc tttgaataaa agtggaagtg
    10381 agagctggac actgtttttc ttgtcgattg tgtcgtatct tttttcaaca tggctatctg
    10441 ctgctgtttg ggtgatctgc ctttcaatca cgagaaaccg gttatcgtct acgattttct
    10501 gataatctcc caaaacttgg taatgatccg tttcaaaatt tccatattct ttttcagctt
    10561 tttcaaaatc tgcttgtcct tcgcgaaagt atttttgatt taatccatga attgttttat
    10621 tttctttctc attttgtgta ttgatcttcg gtacctgaat atcgattttt tcagatcgat
    10681 cagtaaagcg ttcgcctaag atcaattcaa tgactgatcc taagatgggt aaatcttctg
    10741 caaagcttcg aacttgacta ttaaaaagaa ggctacttgt tgtaatcatt acgatagcca
    10801 aagatactcc gaacccttgg agacgtctct ttctccgctt tgcttttata aatgattgct
    10861 cttcgataat aaaacgctgg cggatctcct ctttaagccc tttgggaatt gcttgttctt
    10921 tataagtttt ctttagtttt tttaaatttt ctttttggga atacatggca ctcttctctc
    10981 cttcattcta ggatttgacg taaagcattc aatgagcggt acattttcgt tttaactgtg
    11041 ttttcattca tatccaagat agtcgcaatt tctttcaaac gaaatccttc aaaaaagtag
    11101 aggatcagaa tctctttttc cggactgtct attttatcta aaatcatatg gagctctgca
    11161 acggaagttt ctaaaggtaa ctgttggggg atatcttgaa tttcaaataa gtccacaggc
    11221 gtcctttttt gtctgcgcca actatctaat gccgtgcgga tcaatatccg ataaaaccat
    11281 gcactaaaat gctcgatctc tttttctttt tcaaaatagt tcagggcttt atgaaaacta
    11341 tcttgcatca catctaaggc ctcttcttta tcatgtacgt aattcatcgt gatacggaac
    11401 attttttgat aatcagtttc gatgagtcca ttcagcaggt gacggtattt ttcgatctgc
    11461 cgttttttct tatatccagt aaaaatcatt ctccactcct ttctgcctca cttaatatga
    11521 cgtacaaggt gcgctaaaag tttcaatttt tttatttttt atgtatttac ttgggatttt
    11581 tatatacaaa aaagaaggtg tgacaaaaat cgtgtcacac ctccttattc tgataggctg
    11641 atcgtctctg tcacaacctc tcgtaagaaa ataatgttta ttctttttca gagtcagaaa
    11701 attcttcctc ttcataatct tcatcatcat cgtcttcgtc tatgatactt aaatcttctt
    11761 cgatatttcc tggcaagtct tcttcatcat ctgtcgggtc atctgctccg atttcttgca
    11821 agtcagaatt ataagcagcg atttcttcat cttcatcatc atcgtcatca aacagatcat
    11881 cgtcatcttc ttctgtcaga tctgtatctt ctgggtcatc gtcattgtaa tcgatcgcgt
    11941 cttcatcatc attgataaag gcgttgactt tcttgcgttt acggcgacgt ggagtatctt
    12001 catcctcttc gtccattcca tggtttactt cttcatcgat cgaatcgata gcataccatg
    12061 aacgcaagcc ccaacgatta tcccctaatg agataaagct gccgtcaata ttcaagtcag
    12121 tataaaattg tgcgagtgaa tcacgaattt cactgtctga tttcccaaga tagttttgga
    12181 tctggttagc taaatcagaa aaatccatga catcgccacg ttgttctaag atcgcgtgtg
    12241 ctacttcgat cattgataat tcgcttttgt ttgcaccttc aaatacacta atttccaatc
    12301 aggacacgtc ctttcacagt ctactcctta tcatacaaaa tcagacacaa aaaatcaaac
    12361 ttttctttta tatttacgct gtaaaatcca aggtcaaata atattcgccg attttttccg
    12421 gtcccatgaa cagatcataa tcgattcgaa ccttccctgc tgtgggacgg tctttcaaac
    12481 taacatgcag ttctttcgta aaagtagcaa tctggatcat accgtatggt gttcgatagg
    12541 atgtttctaa ccgttcttta tagccaaatt tcaaacgcat ccgcatttct cctgcacgga
    12601 tcagctgtac atgaccatct ggaactaatt tgatcgttac aggaatctca tcgcccgtct
    12661 cctcctgaat ctctttataa cgtatgtaca gcgtatcgcc aattgtcacc atttgccctt
    12721 tcaaatcaaa ataaaaatct tgcacttccc cttgctgttt tacttttgta cgcaatttga
    12781 ttgaaatcgg tgttccttgt gataattcca ttattcattc tcctttactt ctcatagcta
    12841 cattgttcct agtttctttt gttttgtcaa ccaacttgtc catttttaca gaaaaccttt
    12901 tttccaaaac aactctttga ttttagagta taatattgta gaaaatatga atttatacaa
    12961 aggagccggt tgaatgaacc ctgcgattcg actagatcag cacatttatc aaaaaaacga
    13021 cttttcccct tttgcattaa cagatatatt aacggaatat tctaaagaac accagcaaat
    13081 ctttttacat ttctggcaat atgactgcac agtcatctta ggtatgaagg atgttcgaac
    13141 tccttttcta agtgacggga tccaaacact gaaagatgca ggttacgctc cagtgatcag
    13201 aaattcaggt ggattaggga tcgtatcaga tgaaggaatt ttgaatattt ctttgatttt
    13261 cccccaaagt gacacaaaaa aaataaccat agatgacgct tacgagcaaa tgctcctatt
    13321 gactaaatac gcttttccaa aggcaccgat acaagcattc gaaatcaccc attcttattg
    13381 tcctggaact tatgatttaa gcatccatgg caaaaaattt gccggtatcg ctcagcgtcg
    13441 gatcaaaaat gggatttctg ttatgatgta cttaagtgtc aacggtaatc aggaaaaacg
    13501 cgggcaagtc atgaagcgat tttatcaatc tgctttaaaa gaagactttg gtacaaatgg
    13561 ctatcctgca gtggatcctg ctacaatggc aacacttgaa gaactgcttg accaacccct
    13621 ttttgtaaaa gaagtcaaag aagcatttgc aaaagctttt gaaaagctgt attctgcaag
    13681 tgatttacag gattctaccg actggctaaa aaaagaaatc cagctggagc aatggaacaa
    13741 ccaaatccag agaatggaac aacgtaataa cataaaggag ctacattatg acaacccctt
    13801 ataaatatca aatgctgcct atggaaaaag tttttcgtga tccagtccat aactatatcc
    13861 atgtccagca tcaagtaatc ttagatttga tcaattcgaa agaagtccaa cgtctacgac
    13921 gcattaaaca attaggcact tcttctttca cttttcacgg agcagaacat agccgtttta
    13981 ctcattcatt aggcgtttat gagatttcaa gacgaatctg cgatatcttt tctcgaaatt
    14041 tttcaaaaga aaaaataggc aacggaggct gggatgatca ggaacggctg gtgactttat
    14101 gtgcggcact gttgcatgat gtcggtcatg gtccttactc tcatacattt gaacatatct
    14161 tccatacgaa tcatgaagcc atcactgtag agatcatcac ttctccagaa acagaagtgt
    14221 atcagattct taaccgagta gaagaaggtt ttcctgaaaa ggtagctagt gtgatccaaa
    14281 aaacttaccc caacccacaa gttgtccaaa tgatttctag tcagatcgat gcagatcgaa
    14341 tggactattt gcttcgggat gcttatttca ctggaacaga atatggaaca tttgatctta
    14401 cccgaatatt acgtgtgatt cgtccttatg aaggcgggat cgctttttcc atgagtggca
    14461 tgcatgcagt ggaagattat atcgttagcc gatatcaaat gtatgtgcaa gtgtacttcc
    14521 atcctgtttc ccgtggtatg gaagtaattt tggagcatct gcttcatcgc gcacacgagt
    14581 tgtatcaaga agatgccagc agttttgaac tccattctca attacttctg ccatttcttg
    14641 acggaagctt tactttagaa gaatacctca aacttgatga tggggtcttg ggcacttact
    14701 ttactcaatg gtctgaatct gctgatccta ttttaaatga tttagctaaa cgttttttga
    14761 atcgtaaacc actgaaatct gctacttttt ctggcaaccg ggattcaaaa ttagtccaag
    14821 aactcacttt gttagtagaa aaggtcggat ataatcctgt ttattacact gctgtgaatt
    14881 ctagctacga tctaccttat gatttttatc gcccagaaca aggcagacat cgcacacaaa
    14941 tcgagatttt gcgaaatgac ggaactttga tagaattatc acaggttagt cagcttgttg
    15001 ctgcattagc gggacaagaa caaggagatg agcgattttt ctttccaaaa gaaatggttg
    15061 atccgtcctt gcgtgatcat tatgatttat ttgacgaaac ttatcaagaa tttgccagcc
    15121 atatccgtaa tggcgcattg attgaaataa attaaaatat attggaggaa attatgtcta
    15181 tcaaactagt cgctatcgac atcgatggta ctttattaaa tgagaaaaga gaagtaacaa
    15241 aagaagtaaa agaagcgatt gccgctgctg ttgcaaaagg tgtttcgatc gttttatgca
    15301 ctggccgtcc cttaccaggt gtccaggaac agttaaacga gttgaacctt tttcaagata
    15361 atgattacgt tattacttat aacggggcac tcgtacaaca aacgaaatca ggtaaaatca
    15421 tcgcccgtca cggactgaca catgaagatt tcctagagat cgaagtgatg gcacgccgtg
    15481 tcggcagtca tctccattcg atcgatgaca aagcgatcta cacgcctaat cgagatatca
    15541 gcgcttatac gatccacgaa gcaagtttag tcaagatgcc gctaaaatat cgaacacctg
    15601 aagaaatgac accagacatg aacatcgtca aaatgatgat gatcgacgaa ccagaaattt
    15661 tagatgcagc aattgcgcgt ttaccgcaaa ctttccgtga taaatacaca acggtcaaaa
    15721 gtgcgcccta ttacttcgaa gtactaaata aagaagcaag taaaggtgcg gcagttgcga
    15781 atctcgcaca gcaccttggt attgatcaag atgaaatcat ggctatcgga gataacgaga
    15841 acgacttatc catgattgaa tatgctggtc taggagtagc aatgggaaat gccgtttctc
    15901 ttgtcaaaga agcagcaaat gtcatcactt catcaaacga cgagcacggt gtagctgaag
    15961 caatcaaaaa atatgtactg taataaataa aaaaactgag agaaagctga tctactcgct
    16021 ttctttcagt ttttattttt ttcgatccat ctctcgactg cccatcggtg gctaccacgt
    16081 ttcaatttca caagcgcttc ttgagggctc acccaccaaa ttttattggt cttttcagtt
    16141 ggttctccta tacgcttcca agaatcagca acaaagaaat atcctggatt ataataataa
    16201 gttgcacgat aattcgagta aaaatactca tcagcttctc ccagataaga agaaatttct
    16261 gccacaattc ccatttcttc tatcagctca cggacaatcg cttcttcttt cgtctcttct
    16321 ccttcaattt ccccgccagg aagaaaaaaa gcaccatttg gagcctcgat tactgcaatc
    16381 tgctgatctc tttctatgac aatatatgct gcataacggg tttgataatc agcaaattgc
    16441 tttttttcac caaaaattgg tttatccatg aatagtttcc accttaacag ttttattttc
    16501 tatccatttt acatgagaaa tttctcatga acaagcacaa atcaactatt tccttacttc
    16561 tttttgttat gattgagtaa aaggaaaagg agagatgaat ctatgaaaat ggctcacaca
    16621 tgtgttcgag tgaaagatct tgaagcatca atcgaatttt atcaaaaggc atttggtttt
    16681 gaagaaagcc gccgccgtga ttttccagaa aacaaattta cacttgttta tttgacactt
    16741 cctggggatg actatgaatt agagttgact tacaactatg atcatggtgc gtatgatcta
    16801 ggagacggct atggtcatat cgctatagca gctgatgatc tagaaaaatt acacgagcaa
    16861 cataaagctg ctggtcttga gattactgac ttaaaaggat tacctggcac tgcgccttct
    16921 tactactttg tcattgaccc tgatggttat aaaatcgaag tcatccgagg atagttagta
    16981 tggggcattt tactcttgat tgaataaaaa acaaacacca ataattaaaa tgagacgcaa
    17041 acttttcatt aaattgaaaa agtttgcgtc tttttttaac aaccattgta gctataaggt
    17101 tgccactctc caccaattta tttttaattc atttgaaaga aatcaaataa acgaggtacc
    17161 tttagaaaac aaaactgctt ttcctcctat tatcacacga tcttctttta attcacattc
    17221 tagatccccg ccccttgatg acatttgata agctgacaat tttgttttat tcattctttc
    17281 tgcccaataa ggaataagat tagaatgtgc tgatccacat actggatctt cattgattgt
    17341 taattttgga aagaaggttc tagaaacaaa gtctttttgc acactttctg ctgtcacgat
    17401 gactcctatt cctaattcaa aatttttgat agctgtaaaa tctggctgca atttagccac
    17461 cgttttttca tcttctaata cgaaaaataa atcacgtgct aagtaggctt ctttaatctt
    17521 agccccaatc gcttcttcat attcagacaa tatcggcact tttttcggca taatactagg
    17581 aaaatccatg taataataat ctttaattct tgtcacaaat agattaccgc tttgagtaga
    17641 aaatttaatc gtttcatcag gtattttgta atagttaaat aaaacaaatg ctgttgctaa
    17701 tgtcgcgtga ccacaaagat cgatttctcg atctggcgta aaccatctaa gctcaaattc
    17761 ttggtttttc ttcactgtaa atgctgtttc agataaattg ttttcaatgg caattttttg
    17821 catcgtacct tctggcagcc attcttctag cacataaacc gctgctggat ttcctttgaa
    17881 cacctcatct gcaaatgcat caactacgta ataatctaag ttcattttaa agcccctttc
    17941 tctaatttgt tataaatata ttctgcacaa caaagatctt caatagctag accaactgcg
    18001 tcgaaaatcg tcggcctcga ttctccttta taaaatgttt ttttctcaga aactaattcg
    18061 cctaatgtac caacaataca agattcctct aaatctcctt ctgccatggc tataagcagg
    18121 tccccgcttt ctgttaaggc agctgagtaa tcatctacat agatttgaga agtttccatc
    18181 aacgatgtac ttatctctct tgtatcagga gtaaatgtgc ccacagcatt gatatgtgcg
    18241 tcttccctta cccactcttt ttgaagaaat gcctctttac tagaggtcaa cgtacaaatg
    18301 atttccgctg gacgtactgc atcttctact gatattgcat ttataaacgt tattttagga
    18361 tactgttttt tagctttagc gataaaagta tttcgtcttt cttctaaaat atcgtaaaca
    18421 aacactgtag tgatgggacg aatggtagaa atcgcagtca aatgcgacaa tgcttgttga
    18481 ccagctccaa ttaaagcaag tgtttctgca tgcggatttg ctaagtaatc tgttgccagc
    18541 gcagatacag cagctgttcg aatccaggtg actgcatttg cgtttatcaa tgctttgggt
    18601 agtccgtttt gagcatcaaa caagaaaatc tctcctaaat gagaaggtaa atgatactga
    18661 tgattatccg gaaaagcggt aatgactttt gtgccaaaat atctgttctc ccccaaataa
    18721 gcaggcatca tcgcaaacat atttttgcct tttccgtcag gtaaaatttc caccaatcga
    18781 ggattttggc tgattttacc actttcataa tcaaaaaaac attttttcat tacttgaatc
    18841 gcttcttcaa aactaaaaga agctactact tcctcaaaat taataatttt catagacaat
    18901 tccctcctca caaaagatta acttctaaaa tcaatacaat caatggtaaa attgtatggt
    18961 atacaatttt aaggagtgga gaaaatgcct attaattctt atgacaatta cccgttaact
    19021 tggcgaccag acaaacaaaa attgaagcgt ccttattatc gatcgttgat tcaacaatta
    19081 gaacaagata ttttaaagaa taaattacag aaaaacactc agttaccctc ccaaagagag
    19141 ctagctgatt atttagattt aaattttact accgttggca aggcttataa atatggaatt
    19201 gagaaaggtt tgctgtatac gaatattgga agtggcacat ttatttctcc caatgtcttt
    19261 gagtccatca ccatctctac caatgaagta accgaacata tcattgattt tggtctggtt
    19321 agttcttttg aagaatgtaa taaattcgtt gtcccgttta ttcaatcaac tagtaataac
    19381 ccaaatcttg ttggtttgct taattaccaa gatcccaaag gcacaaattt tcaattgact
    19441 actgcagcaa attggttaaa aactcaagga gtcattccgt ccttagaatc tctatctatt
    19501 gtttccggtg ttcaaaatgg attggctatc acactttctg cacttttctc tccaggtgat
    19561 agaatagcaa cagaccgata tacatacgct aatttcattg aattggctca gttatatcat
    19621 ttagagatcg ttccaattga ttttgacgag caaggaatgc gacctgatat attgattcag
    19681 gaatgtagaa aaaagaagat tcatggtgtc tttcttatgc cttcttgtaa taatcctata
    19741 gggtttcaaa tctctcacaa aagacgagag caattagccg aagtgattga aaaagaaggt
    19801 ctatgggtta ttgaagatga tatccattca tttttaacaa cttactatca acatacgatc
    19861 attcctcctt tccaaaattt attacctaac caaacaatct acttagctgg aatgacaaaa
    19921 tttatttgtt ctggattgcg agttgcttat cttgtagttc cagtacatgt ccaaagcaag
    19981 atagaacaag caatttttaa tataaatgtg aaaacttctg gattagatgt ggaagtcatc
    20041 actcaaatcc tccattcaaa tgaagcaaat aggatcttag aaactaaatt aagtcttaca
    20101 aaaagagcca atcagctttt tgattctctt ttcaaagtag aaaaaccaac gaatcgtgtc
    20161 ccttactatc gaattcttcc agttgatcct gagattgacc ctaagataat tgagaatgac
    20221 tttcttgcta acggtatccg actttatcat tccagacgct ttactacacg tacgcaacca
    20281 gatgcattcg ttcgaatttc cctatcctct aataaccttg aaattttagc ttctggacta
    20341 agtatcgtaa aagaaaaaat aaaaaaatat gaattataac ctattgataa ataaaggaat
    20401 caaatcttat tgattccttt caataaaaaa gaggcactac ccgaggttca acaacgggta
    20461 gtgcctcaaa aaataaacga acattttgca cttaaagtat ccaagaagca agaaaaatta
    20521 ttttattttc ttattttact aaatcactag gtttctgtca gaagatgaaa ttatccaatc
    20581 atgacacttt cttctggata tttgtatttt gattgtgttc tttgtgcttt tgtcatcaga
    20641 gagaagacca cagtcaatac accaactcgt ccaatgtaca taagcgctat aatgatcaat
    20701 ttccctattg cagtcaagtc tggcgtcaat cccatcgtaa gacctactgt accaaacgca
    20761 gagaagacct caaaagcaat atattcgatg ccagaagttt taggtatcgt ttcagtcacc
    20821 gataaaatca tgatggcgac tacacacaac gtcaacgtaa caaaaaataa agtaagtgcc
    20881 cgcaacactg cgccttgtcg gatggtacgg ccaaatgctt ccgctctcgt acgtcctttc
    20941 aacatcgaac gcatctggat cagcaacaca cctaacgtag ttgttttcaa tccgccggcc
    21001 gtcgacccag atgttccgcc gatgtacatc aaaaacatcg ttaaaatcag acctgcatgg
    21061 ctcatatgga ggtaatcaac tgaataatat ccagccgttc taggtgtcac actcataaaa
    21121 aaggtgtttg ccaatcgttg aaacatattc ccttccacta gcgttgcccc attgcgctct
    21181 gtgaaaaaga acacaacgaa tccgcctaat aaaagggtac ctgtaacggt taatgcgatt
    21241 tttgaatgaa tcgtcatctt ctttttctga tgatattcca gtaaatcacg ccatacgatg
    21301 aagcctaacc caccagaaat gatcaaagca gataccacca tgatcaaata aacattttgc
    21361 tggtgattgg ctaaactgtc tcctaacaaa tcaaaccctg cattacaaaa actagagatt
    21421 gcatggaaca aactgtacca gatacctttc aaccagccaa attcaggcac aaatacaaaa
    21481 gataaagcaa ccgtaccaat caattggatc acaaaggcca attttaggat gtaaagcatg
    21541 agcttcatca cacctgatac ttcttctaga ttcagcgctt cttttaatat aatacgtgtg
    21601 ctgagactga ctttcttctt tatcaatgca aagcatagaa tcggaaccat cataaaacca
    21661 agaccgccga tctcaatcaa aagcatgatc aaaaattgac cagcactgtt ccaatgctct
    21721 gctgtattca atgttgtcaa tcccgttaca cagacagcag aagttgcagt gaaaagcgca
    21781 tcgataaaat ttgtcccatg cccactatta ctaaaaaatg gtagggtaag taggccgccg
    21841 ccaactaaaa tcaggagaaa aaatcctaat gcgagaagct gaacagccgg caaacgtttc
    21901 tttatttgtt tgctttgttt aatcatcgtt ttcctctttt aaaaaaagat accacttagt
    21961 cagtggtacc catatttttt actttgatca accttgttac ttctgcgcgc tcgttttctt
    22021 ccagcttcat acgaatataa tagatcgttt cttctaattg agggatcgtc atatattcta
    22081 atgcattgac tcgccgacgt gtcttttcga tttcactcgc catcagttga caagtttttt
    22141 cgatttctgt cagttccaaa agttttggaa gcagggtagt aaaccgatcg attgaccgat
    22201 ccagttcggc actggaattc aaatacccgt attccaaagg tgtgtctgtc agctgttcat
    22261 catattcaaa attcatcaca ggtactttta cactcatgat atttttgtct acgatagaca
    22321 aatcaacgtt ttccgccggc aaaacaaata attcttcgat gaaaggttcc gtgatcgaag
    22381 catttgccaa tacaaaatca gacattgctt ctgtcataga ctcttccatt tcttgacgca
    22441 gctggttgtt ctttcgaatc aacaggatga attgacgcat cagttcatct tgcttatctt
    22501 ttaataattt atgccctctt gtggcagttg tcaactgttt tttcaatcga gtcaattcca
    22561 tgcgggtcgg attcacattc aatcgtgcca tcagaactca cttcccttct ggcagatatt
    22621 tgtccagcat gtcgtctttg attcgtttta attcggttct tggcaacatc gatagcagtt
    22681 cccaccctag atcaagtgtt tcagtaatcg aacggttcgt gtagaatcct tgattgacat
    22741 attctttttc aaaccgctca gcaaacttcg catagatctt gtctacatca gataaagcag
    22801 attcacctaa aactacggct aattcttttg cctgttttcc ttgtgcatag gctgcaaaaa
    22861 gctgattcat cgtagctgca tggtcttctc ttgtctttcc gggtccagtt cccttatctt
    22921 ttaaccgcga aagagacggg agtacatcaa ttggtggctg aatcccactc ttgtaaagct
    22981 ctctagtcaa gatgatctgt ccttctgtaa tataaccagt caagtcaggg attggatgag
    23041 ttttatcgtc ttctggcatt gttagaatcg gaatttgagt aaccgatcct tttagtccgc
    23101 ggattcttcc tgctcgttcg aataaagtag ctaagttcgt gtaaagatac ccaggatatc
    23161 ctcttcttcc tggaacttct cgccgtgctg cagaaatctc tcgcaatgcc tctgcatagt
    23221 tggtcatgtc tgtcatgata accaaaacat gcattccctt ttcataagca agatattctg
    23281 cagcagtcaa agccatacga ggagtagcga tacgttcgat tgcagggtcg tttgctaaat
    23341 tcataaacat gacagaacgg tcgattgcgc ccgtttgacg aaaatcttcc atgaaaaatt
    23401 cagcttcttc aaaagtgatt ccaatagcag caaagactac ggcgaaatca tctgatgaat
    23461 cgagtaccgt tgcttgtcgc gcgatctgag cagctaattc tttatgagga agaccagagc
    23521 cagaaaatac tggtaatttt tgcccacgaa ctaatgtatt caaatgatcg attgcagaga
    23581 tacctgtttg gataaactca tctggataat cccgagctac tggattgatt acttctccgt
    23641 taatatccaa atgcttttca ggcaggatct ctggtccgtt atctttcggt cgtcccaaac
    23701 catcgaatac tcgaccgatc atgtcttccg atacacctag ttctaaagga tggcctaaaa
    23761 atcgtacaga agagtcacgt aaattgatac cacttgttcc ttcgaatatt tggaccattg
    23821 ctttatcttc ttggacttcc aatacttgtc cacgacgaat ctcgccattt tgcatacgga
    23881 cttcgattaa ttcttcatat ttgacaccag caactttttc aaccgccatc aatggaccga
    23941 cgacttcacc gatcgtacga tattctttaa tcattgctca tgcctccttc tgaaacgatc
    24001 gtttgcatcg tttgcttgat ttcaccaata atttgatcta gtttttctaa ttctgtttcc
    24061 gggatatatt tgcttcgtcc gatcctttca cgaatcgata cggtaccgtc catgatctca
    24121 cttaaataag cacctaacga caacgcacgg cgtccttctt caccaaaggt caagatcatc
    24181 ttcaacattt tgaattgttt ctctcttgaa gtgaatgtat cgacatcatc aaaagcattt
    24241 tgctgcaaat aatcttcacg aatggatttt gccacttcta atgtcagacg gtcattatct
    24301 gaaagggaat caatccctac caaacgcacg atttcattca gctgctgttc ttcctgcaaa
    24361 atacgcatgc cctcggtgac catggatgcc cagtcagctt gaaggatatg atccatatac
    24421 ttccctactt ctgttgaata aagagaatag ctttgtatcc aatcgatcga aggaaaatgt
    24481 cttttttggg ctaaactgga atcaagaccc cagaatactt ttacgacccg caatgtattt
    24541 tgtgtgactg gttccgaaat atcccctcca gacggggata cagcactgat tgcggttata
    24601 cttccttcgc gaccttctga tcctaatgca atgactcttc cagatcgttc atagtattcc
    24661 gcaagccgtg aaccaagata agctggataa ccttcatcac caggcatttc ttccaaacgt
    24721 ccgctcatct cacgaagtgc ttctgcccat cgtgaagtgg agtctgccat gatagcaacg
    24781 tcataaccca tgtcacggaa atattccgca attgtgattc ccgtatagat agaagcttct
    24841 cgagctgcta ctggcatgtt tgatgtatta gcaatcaata ccgtacgttc catcaacgat
    24901 tctcctgtat tagggtcaat caattcaggg aattcattta aaacatccgt catctcattt
    24961 ccacgctcgc cacatcccac atagacaacc atgtccacat cggcccattt tgcaatttga
    25021 tgctgaacga ctgttttccc tgcaccaaaa ggaccaggta cagctgctgc tcctcctttt
    25081 gtcactggga agaatgtatc aatcactcgc tgtccagtga tcatgggtgc atccgggttt
    25141 aatttttctt tgattggccg actacgtctt actggccatt tttgaagcat cgtcaaattt
    25201 ttgtcaccat tttcggtttc aatgacacat actgtttcat caatagtgaa tgaacctttt
    25261 tcgattgttt tgatcgttcc ttttatccca tttggcacca tgattttatg ttttataact
    25321 tttgtttctt ctacgatccc gataacatct ccagcactca ccgtttctcc ggcttctact
    25381 gttggttcga accaccattt tttctcatgg tccaatgcgg gcaattgaac tccgcgccct
    25441 aagaaattac tttgtgtgat ttccataaag gtatctaatg gccgttggat tccgtcaaac
    25501 atttgtgaaa tgatccctgg tcctaattca acagataaag cttcaccagt agagcgaacc
    25561 ggttcgcctg gaccaattcc agaagtctct tcatatactt gaatagacgc cacatcgcca
    25621 cgcatctcga taatttctcc gataactcct agatctccca ctagacacat gtcttggata
    25681 ctggcatcag acatgttctc agccattaca agaggtccgg agacttttat gatttttcca
    25741 atttgcaaaa tttctatctc cttttcatac tataagatat tttgacctac tgctttttct
    25801 acgttttttt gaatttcttc cagccctatc cctaatgatc cttgatgatt gggaatcaat
    25861 acgatagcag gtatcatttg ttccttgtaa cgttcgatcg tttctgggct caatgctgca
    25921 cattgctctg tgacataaat cacgccatac tcattctttg ccatttcttc aattgcctga
    25981 cgaacggttt gcttggttgt accataacga acatcaaagc caaacaattt gaatggcaaa
    26041 acagaatctt tgtcgccaac tacaccgatc ttatgcgtca tagacttgtc tca
//
LOCUS       DAYLZO010000025        25790 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.025, whole genome shotgun sequence.
ACCESSION   DAYLZO010000025 DAYLZO010000000
VERSION     DAYLZO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.025"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            369..1559
                     /locus_tag="ACGVJG_000977"
     CDS             369..1559
                     /locus_tag="ACGVJG_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFJ6556951.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1693..3882
                     /locus_tag="ACGVJG_000978"
     CDS             1693..3882
                     /locus_tag="ACGVJG_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6556952.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3879..5672
                     /locus_tag="ACGVJG_000979"
     CDS             3879..5672
                     /locus_tag="ACGVJG_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFJ6556953.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            5872..6186
                     /locus_tag="ACGVJG_000980"
     CDS             5872..6186
                     /locus_tag="ACGVJG_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFJ6556954.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            6207..6584
                     /locus_tag="ACGVJG_000981"
     CDS             6207..6584
                     /locus_tag="ACGVJG_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFJ6556955.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            6594..7367
                     /locus_tag="ACGVJG_000982"
     CDS             6594..7367
                     /locus_tag="ACGVJG_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556956.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            7389..8792
                     /locus_tag="ACGVJG_000983"
     CDS             7389..8792
                     /locus_tag="ACGVJG_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFJ6556957.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            8974..10158
                     /gene="mobT"
                     /locus_tag="ACGVJG_000984"
     CDS             8974..10158
                     /gene="mobT"
                     /locus_tag="ACGVJG_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFJ6556958.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            10155..10445
                     /locus_tag="ACGVJG_000985"
     CDS             10155..10445
                     /locus_tag="ACGVJG_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556959.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            10442..10663
                     /locus_tag="ACGVJG_000986"
     CDS             10442..10663
                     /locus_tag="ACGVJG_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6556960.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            10705..11484
                     /locus_tag="ACGVJG_000987"
     CDS             10705..11484
                     /locus_tag="ACGVJG_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFJ6556961.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            11545..12045
                     /locus_tag="ACGVJG_000988"
     CDS             11545..12045
                     /locus_tag="ACGVJG_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFJ6556962.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            12101..12739
                     /locus_tag="ACGVJG_000989"
     CDS             12101..12739
                     /locus_tag="ACGVJG_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFJ6556963.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            12811..13206
                     /locus_tag="ACGVJG_000990"
     CDS             12811..13206
                     /locus_tag="ACGVJG_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFJ6556964.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            13190..15643
                     /locus_tag="ACGVJG_000991"
     CDS             13190..15643
                     /locus_tag="ACGVJG_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6556965.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            15640..17667
                     /locus_tag="ACGVJG_000992"
     CDS             15640..17667
                     /locus_tag="ACGVJG_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFJ6556966.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            17664..18686
                     /locus_tag="ACGVJG_000993"
     CDS             17664..18686
                     /locus_tag="ACGVJG_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFJ6556967.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            18703..19632
                     /locus_tag="ACGVJG_000994"
     CDS             18703..19632
                     /locus_tag="ACGVJG_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFJ6556968.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            19946..19993
                     /locus_tag="ACGVJG_000995"
                     /pseudo
     CDS             19946..19993
                     /locus_tag="ACGVJG_000995"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20009..21928
                     /gene="tet(M)"
                     /locus_tag="ACGVJG_000996"
     CDS             20009..21928
                     /gene="tet(M)"
                     /locus_tag="ACGVJG_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFJ6556969.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            22026..22214
                     /locus_tag="ACGVJG_000997"
     CDS             22026..22214
                     /locus_tag="ACGVJG_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFJ6556970.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(22272..22625)
                     /locus_tag="ACGVJG_000998"
     CDS             complement(22272..22625)
                     /locus_tag="ACGVJG_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556971.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            23156..23578
                     /locus_tag="ACGVJG_000999"
     CDS             23156..23578
                     /locus_tag="ACGVJG_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFJ6556972.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            23575..23805
                     /locus_tag="ACGVJG_001000"
     CDS             23575..23805
                     /locus_tag="ACGVJG_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6556973.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            24303..24503
                     /locus_tag="ACGVJG_001001"
     CDS             24303..24503
                     /locus_tag="ACGVJG_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFJ6556974.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            24530..25723
                     /locus_tag="ACGVJG_001002"
     CDS             24530..25723
                     /locus_tag="ACGVJG_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6556975.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         8919 a         4184 c         4936 g         7751 t
ORIGIN      
        1 gttttcaacg taaagtgtat aaaacagaat gcgatactat caagggagtt aaaacaatat
       61 acagaacagt aaaacactat aaataaaaat aaaagtgcaa taaaagtgca atttttgcac
      121 ctaatcatac tctgtttata tatgaaaact agattctgaa tccaatattt ggttaacgag
      181 aggcgttcga atttgaacgt cttttatata ttactttcaa ataaatatga ggtagaatac
      241 tatctatttt ctttttaacc caattgttat aatggagagt gtaaaatatt ttgtgtaaat
      301 agaaaaaagg aagtcccttc tgtagaatag agttaccaca acacattcac agaagagagg
      361 acttccctat gaacgatttt actacagaaa ttctaaagac tctagcgaac aaaggcgatt
      421 tgaatgaatt attccgtgtc catttggaga aagcagtcaa tacgcttctc aaaacggagt
      481 taacggcttt cctagattac gaaaagtatg atcgcattgg ttttaacacg ggtaattctc
      541 gtaacggctc ctatgaccgt acggtcaaga ccgagtatgg ggaacttcat ctccagattc
      601 cgcgcgaccg caatggtgag ttcaagcaac agactgttcc tgcttataga cggacgaatg
      661 acacgttaga ggagaccgtc attcacctct tccgaaaagg tattaccatg tcggaaatcg
      721 cagacttgat tgagaaaatg tatgggcatt actacacgcc ccaaaccatg tccaatataa
      781 caaaatcatt tacagaagag gtaacggcgt ttaatgggcg ggagcttcat gaccgttatg
      841 ctgctattta tatggacgca acgtatattc cgttaaagcg gaaaaccgtc gccaaggaag
      901 ctattcatat cgcagttggc attcgcccgg acggatcaaa ggaagtattg agctatgcga
      961 ttgcaccgac tgaatccatc acgatttggg aggaaatttt attggacctt caagagcgcg
     1021 gtttgaaaaa tgtcctcctg ttcatcacgg atggcttaaa ggggatggta ggagcgatta
     1081 gtcggttcta tcccaaagct cgttttcaac attgttgtgt acacgtttcc cgtaatatca
     1141 gtcataaagt gcgtgtcgat gatcgtaagg aagtctgtga tgattttaaa atggtgtatc
     1201 aagcctcatc taaagaggtg gcgttggaag cacgtggtac ttttgcggaa aaatggaaaa
     1261 ccagctatcc aaaagtggtt gaatcgattc tttcgaacga tcacttgctc actttctatg
     1321 atttcccctt ggccatacgc aagagtattt attctacgaa cttgattgaa tcctttaata
     1381 aacaaatcaa gaaatacagc caccgcaagg aacagttcca aaatgaagag tcgatggaac
     1441 gtttcttagt ctcgtctttt gatacttaca accaaaaatt cctaggtcgc agtcataaag
     1501 gtttccaaca ggccgaaggc gagctcgaac aaatgctaag ccaactgatt gagaattaga
     1561 aacaatctgc agtagggaag aatcatttac acaaaattat tgacgctccc ttataatgag
     1621 aacttttact aacataattt atgataaact tactatataa tcatattaat taaatagtta
     1681 ggagatctgc ttatgtttat atctactctt caaattcgaa attttagaaa ttatgaatcc
     1741 gaaatctttc gattcgataa tgagacaaat actattatag gtgagaatga ttcagggaaa
     1801 accaatgctc ttactgcatt gagaatctta ttagatgata gttattacta tagtagtaaa
     1861 acactaaaag aatcagattt ttttcatgga attcaaaatg ggtggcaagg gcattggatt
     1921 attatctctg cgacatttga aggaatcagc gaagaagaat ttgacaatga gatttgtgct
     1981 tccatttctt taaattctga aagtcaaaca atcttagaag aattgatttc aaatgcagat
     2041 aaaggtgtag gtagtatttc tctatttatt agacctaaca aagctattag aaagcaatta
     2101 tttgatattt ccactgaatt agactctcat caatttaacg aatttagaaa ttctattcgt
     2161 ctatccgact atgaatttta ctacacatct aaatcaaatc taaatttctg tatcaatgaa
     2221 aattatgata aattggtagg aagactcaat gacaatatag ctagtaatcc tgaagaagac
     2281 gatgaagctc tgttgggctc aagaattgac atgtcagata ttttcaagca tatttcggtt
     2341 gtttatgttg acgctcttcg agatgttctt agagaaatga aaaacaatcg aaacccagtc
     2401 aaaagaatca tggaaacaat tgaatcaaaa atatcatcag ataatgttga gtcattaaaa
     2461 acaattatac agcaattaaa tgagactatt accagtgttc ctgaaattag gaaaattggc
     2521 gaaaatatta atcggcaact aaatagcata attggttcag tgtattctcc taatcttctt
     2581 ctttcctcaa ctatgtcaga tgatatgggt tctttagcaa aatttctttc aatgaagcca
     2641 gaacaaaata tcgacttaga cttactaggg ttaggacatc taaatatgat ttatttagct
     2701 ttgaaaatag tcgaatttga agcgtgtcgt tcaagagaac tacttaatat tatgttgatt
     2761 gaagaaccag aagctcatat tcatcatcat attcaaaaaa ctctgttcga aggattaaat
     2821 cttcaaaaaa actatactca gattctcatg accacacatt ctgtacattt agctgaagtg
     2881 tctgaaatat ctcggatgaa tgtattaaaa tcgtttaatg gcaaatctat cgttatgaat
     2941 ccagcaaata atctgaactc atttgctaaa gaaaagttaa aaaagaacaa tctaaatcta
     3001 ataaacgcag tagagagata tttagatgtt aaaagaaatg gattgctttt ttctaaaggt
     3061 gttatcttag ttgaaggaga tgctgaagaa atacttattc ctcaaatagt aaaaaaatta
     3121 tttggtataa acttagacga acttggaata ggtttgatta atgttggtag tacttcgttt
     3181 gaatatatcg ctagcttgtt tgacgatgat agaatacaga gaaagtgtgc tattgtaact
     3241 gatttagatg aacaggcaat cccatcagaa catcgattgt ataaaagcac tgcacaagag
     3301 aaaggtcaag caagaaaaga aaaattatcc tcccttttta atgaaaacaa ttgggttaat
     3361 tgtttttatg ctaatacaac atttgaaatt gagtttttag atgtgaataa agaaaatatt
     3421 ctaacttatg tcagtccaat tatagatgac gcattcaaag ataaaaaaac aataactgct
     3481 tataaagaag aaatacaaag tgaacaatat ttagaaagaa atttagcgat gttaaaacta
     3541 gccaatcatg ttggtaaggg gtggtttgca attgagttag gtgaaaaaat tgacttttta
     3601 atcaaaattc ctaagtatat tcttaatgct attgcttttg catgtcagga aatagtagat
     3661 ctctcaattt ataaaaaaat tctactccat acaatcgact tgtacgagga aactacagaa
     3721 ttgtcagctt tatcagctag tttaaaaaca agtgatacag ctgatttgaa tatagcaata
     3781 atttctttat tagagtactt tggtaaagat gaggatgttg tgtatctaat caatcagata
     3841 gaaaattaca caaaaatact ttatgctgag gtttcaaaat gacaaatatc ttcgaaaaaa
     3901 tcgtaaatga tttaaattta gaacaaaaag atgttgtgga aagtgaagat aacctttatg
     3961 taaatgcatg tcctgggagt gggaaaacaa gggtacttac aaggaaaatt gcttaccaag
     4021 caattaaaca cgatggatca ctaaaaagaa taattgctat cacatacact aatagagctg
     4081 ctgaagaaat taaggaacgc ctaagtttac ttagtataga tgatgatatt aatatttggg
     4141 tggggacaat acatcagttt tgtcttgaat ttattattta tccttttaaa atgaatctct
     4201 caagagtatc aaaaggattt acaattattg atgattatac tcaaagagct tacgtcagcc
     4261 aaattaataa tatgctgaat ttaaatctca aaacgtatga actgaacaaa attgatttgt
     4321 cactttctca tgattttaaa attaatgaag ttcagtatac tcaagtttct gaaatgtatc
     4381 acactatgtt ggaaaaaaat aaagaaattg attttgatat gattttacta gtagcgtata
     4441 aaattttaac tgtaaacagt aacgttgcac gtaatattgc tagtaatata agggcgattt
     4501 atgtggatga atttcaagat actagagaat tacaatacaa tgtaatagct aaattacttc
     4561 aaaacaaccc tcaaattcag tcaatgtttg ttggagatat tgaccaagca atttatggat
     4621 cattagatgg tatagctaaa tcagttgaag aactagaaat gctaactagt cacacttttc
     4681 aatcaaagac gctacatggc tgttacaggt ctaatcagag attagtagac ttctactcta
     4741 attttcagag ttgtccttac gagattgaaa gcagagggaa taataaaaac gaccgtggtt
     4801 ttataagtta tgattgcaaa gttactaagg aggatttacc caacataata aaaaatataa
     4861 taaaggaaaa gattgaaagc ggcattcatg aaaaagatat ttgtgtactt gctcctcaac
     4921 actatcctct gttttcactg ggtgagtatt taaagaaaga attaccgtat tgcaattttg
     4981 atgctataaa tatatcacca attaaggtaa ataatcatag tttatttttc aagttagcaa
     5041 ttttgtactt tactgaatca ggtgaaaagg ttcgaagaag aaagctaatt gcaaacgata
     5101 ttttaattat attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag
     5161 atttattaca gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg
     5221 atttatacaa atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt
     5281 acccgtctat ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa
     5341 ataattcatt atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta
     5401 tcacacttac tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta
     5461 atcttctaaa cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa
     5521 ttagttcagc gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc
     5581 ttttctcaga ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat
     5641 tagataaaat agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata
     5701 tagtaggaat cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag
     5761 tgaacttatg aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat
     5821 cacacaaata agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt
     5881 aaatatgtca ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg
     5941 aacattgaac aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta
     6001 tactcaacga ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag
     6061 aaacattttg aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt
     6121 tataaaattg gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa
     6181 gcataagaaa ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag
     6241 accagacatt tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg
     6301 aagatggtac ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac
     6361 aaggacgcat gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca
     6421 acgcagaagt ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag
     6481 gggcagacgt tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac
     6541 ctcatcagaa atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa
     6601 acacaccatt gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat
     6661 accattattc aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag
     6721 aaaaattatt aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca
     6781 aactctttcc cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt
     6841 ttcttccaga aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta
     6901 agtggttgaa agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg
     6961 gaaaatgtgg ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa
     7021 gtgtctatcg tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat
     7081 tagaggaaaa tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt
     7141 gcgaatacaa agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga
     7201 caaaagaagc gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag
     7261 aaaaaaattt acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac
     7321 caacttttga taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga
     7381 ggatttttat gccattgctt catgacagag gagagcgaat caaagccaac gataagcact
     7441 tggtctatca ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt
     7501 ttcattggag agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct
     7561 atcaacttca tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac
     7621 ttatcagctg gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc
     7681 aaaagttagc tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag
     7741 agggattctt taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta
     7801 aaatatatta tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga
     7861 aatatcaaga tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat
     7921 tagtggaaaa aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg
     7981 gcaatcgaat tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat
     8041 cggtctattg ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg
     8101 gaaaatccta ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt
     8161 atattcttga ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct
     8221 attaccgtaa agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc
     8281 gcagtgaaac catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg
     8341 gtttgtctgc caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc
     8401 gtgaaagtac caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag
     8461 gcttcttttt aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc
     8521 gggacaactt taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga
     8581 tgttcggaag cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg
     8641 tggatacagg aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg
     8701 atttcttagg agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg
     8761 aagtgaaagg tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct
     8821 ttagccccct ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca
     8881 accaatgttg atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat
     8941 agtttacatc ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag
     9001 gaattgaaag aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct
     9061 catattacca gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag
     9121 gtgaaagaag atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg
     9181 ttattcgact atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag
     9241 gttttacgtt tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc
     9301 gaacactatc gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt
     9361 ttattagaac tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat
     9421 cggagctggt atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg
     9481 gatttagcta tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt
     9541 aaacaggaag aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg
     9601 gttcaccgtg atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt
     9661 gaagtctatt tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc
     9721 ttagaagacg cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc
     9781 caagccatga aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc
     9841 aatcgctata tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact
     9901 aatgaacgtt gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag
     9961 ccagagcctt attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca
    10021 ttaaaaatcg caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt
    10081 caggaagcca aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg
    10141 gaggatttga ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg
    10201 gcattgagtt tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg
    10261 gaaaacggca agctggtgat accatctata cctatgattt ttacaaggtg agattttata
    10321 ataattatac caatcgagta accgtctatg gagagcattt aacccctttt caattattaa
    10381 ggcgtgtaaa aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca
    10441 catgaatttt gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt
    10501 gttagcgatt gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat
    10561 tggcttcttg attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa
    10621 agatgtgtta ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat
    10681 aaaataggaa gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa
    10741 tttaaatctc ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga
    10801 ggtaacgaga ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat
    10861 tttacttatt ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata
    10921 agagttcctt ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca
    10981 gcaagctcca tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca
    11041 cttccagaga attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca
    11101 caagctattg aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac
    11161 agagttcaca ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca
    11221 gttcctagag acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa
    11281 gaaattcaac ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga
    11341 atgattagct ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat
    11401 attattggaa agagagaagc aaaactaata ataggtattt caagtacagt tacagattat
    11461 attagcggat accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt
    11521 tttataaaaa ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg
    11581 tataatgaag gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg
    11641 gcagaaagaa tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt
    11701 ccttttgatg tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt
    11761 caagaaatcg aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt
    11821 agtagcttgg aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat
    11881 tcgatggaag atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct
    11941 tccaatttac aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt
    12001 aattacctag ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg
    12061 ttataattaa attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa
    12121 tcttaaacta ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt
    12181 tcgttacaaa cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt
    12241 tccagaatat gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct
    12301 agctgtctat gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt
    12361 tggtatcgaa tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc
    12421 tcgatttatt ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg
    12481 tttctcatta agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag
    12541 aagaaatcct ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa
    12601 aacgagattg tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt
    12661 cagatattca atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac
    12721 aaaaaataaa attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt
    12781 tttctatttt tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt
    12841 tggtcagtag aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc
    12901 tttacgcaaa tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta
    12961 ccaccacttt ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc
    13021 actgggttca tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct
    13081 atcattacct atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa
    13141 aaagaaacaa tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc
    13201 gattaaatat atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta
    13261 tgaattaatt ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga
    13321 taatttctgt caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc
    13381 aaccgaatca agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt
    13441 aaaagagata gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg
    13501 tgacaatcaa gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga
    13561 agtatccgta aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg
    13621 tgtgaatcac aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt
    13681 ttctaagatg gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt
    13741 ggagaaagat gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt
    13801 tgatgactac gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa
    13861 atacgatatt ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat
    13921 tgaacaggaa gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga
    13981 attggatttc cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt
    14041 tgataccagt atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa
    14101 taaaaagaaa gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag
    14161 tactaatgtg gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac
    14221 caaagaatct atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga
    14281 gctgaaacaa cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt
    14341 acgacctttt ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata
    14401 tatcaatgac tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc
    14461 tacacaaatg attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag
    14521 aaatatttat ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc
    14581 attagcgtcc gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct
    14641 ggtctattat tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg
    14701 tggtgaatgg aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac
    14761 gagtgagaac gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga
    14821 ttcagaaagc cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag
    14881 cgagaagttt cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg
    14941 gttgttattg gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc
    15001 tgaccatatc gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag
    15061 tgtcacccaa tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt
    15121 cttacccgat tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc
    15181 aatgctgatt gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt
    15241 taaaattgtt gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct
    15301 atctatgcga ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca
    15361 aaacgcggac gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt
    15421 tcgttccact gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga
    15481 agatgaaaac aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga
    15541 catctatggt cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc
    15601 ctttgatacc agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa
    15661 tttcttcact atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc
    15721 acgctgggca atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat
    15781 gaatactcca agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg
    15841 ggctggcttc catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat
    15901 gccattacta attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg
    15961 gtgaaagaag cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac
    16021 atgcagacga ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt
    16081 ttcctccttc tttttattct cattgttgga atctatgttg cctatacagg attggttaag
    16141 cgagaaacga cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg
    16201 gcttccttta ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat
    16261 gtcagtaccg ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa
    16321 gggaaagaca gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca
    16381 tggctattgc ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa
    16441 agtttattgt ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt
    16501 gaagaaattg aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta
    16561 ggaacagtct tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta
    16621 acaggaatca tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc
    16681 agtttcctat taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag
    16741 ttatttaatg tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt
    16801 atttccacta tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta
    16861 caaattgtga cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact
    16921 ttacaaagca acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg
    16981 atgaatcgac aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca
    17041 gggtcaacga agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat
    17101 actcgtcaaa atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag
    17161 caacaaggcg gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa
    17221 aatcgtctag tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat
    17281 gtcaaatatg cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa
    17341 caatctatgg cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa
    17401 aaacgaaaga acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga
    17461 aaagaaaacc catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa
    17521 accgtcacgc ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa
    17581 aaagagcaaa aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga
    17641 acggataagc gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct
    17701 tctctctctt cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca
    17761 gtggcgaaaa caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag
    17821 tgttggcaca ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg
    17881 tttcttatat cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc
    17941 aaagttccga atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac
    18001 aaggggttaa atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag
    18061 attctgttat ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg
    18121 ggaaaaaata tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa
    18181 aagcagacta tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg
    18241 gaaatcagtt ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat
    18301 ttgatggtga aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct
    18361 atgtctttgg aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg
    18421 tgtatgacaa agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc
    18481 aagaaatctc tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata
    18541 atgcaggcac atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg
    18601 caggcgaccc aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg
    18661 gggctagacg tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag
    18721 cgaaaagaaa agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt
    18781 ccgatgatta aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt
    18841 ggtagtgtcg gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt
    18901 gaaacagaaa tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa
    18961 tcctttgcga aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat
    19021 gaaaaattga cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt
    19081 aaggatattc ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg
    19141 aatgagaaca cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac
    19201 aaggaaacta tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg
    19261 gttattatca aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa
    19321 cagctagaaa gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta
    19381 gaaacgttct tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt
    19441 aatcatgtgt tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc
    19501 tttaccagaa aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa
    19561 acaaaggcaa cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag
    19621 attgtaaaat aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt
    19681 tgataaaaaa ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact
    19741 atttaaaagg agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata
    19801 gcgggaacaa ataattagat gtcctttttt aggagggctt agttttttgt acccagttta
    19861 agaatacctt tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc
    19921 tatggttatg cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct
    19981 tttgggtttt tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca
    20041 tgttgatgca ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac
    20101 agaattagga agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca
    20161 gagaggaatt acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa
    20221 catcatagac acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt
    20281 tttagatggg gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat
    20341 attatttcat gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga
    20401 ccaaaatgga attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga
    20461 aattgtaatc aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga
    20521 atctgaacaa tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc
    20581 cggtaaatca ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg
    20641 ttctctgttc cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat
    20701 agaagttatt actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg
    20761 aaatgttttc aaaattgaat atacaaaaaa aagacaacgt cttgcatata tacgccttta
    20821 tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt
    20881 tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg
    20941 agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa
    21001 actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga
    21061 accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag
    21121 tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt
    21181 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
    21241 agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac
    21301 cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc
    21361 gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca
    21421 atcatttcaa aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg
    21481 ttggaatgtg acggattgta aaatctgttt taagtacggt ttatactata gccctgttag
    21541 tactccagca gattttcgga tgcttactcc tattgtactg gagcaagcct ttagaaaagc
    21601 tggaacagaa ttgttagagc catatcttag ttttaaagtt tatgcaccac aggaatatct
    21661 ttcacgggca tataacgatg ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa
    21721 aaataatgag gtcattatta ttggagaaat tcctgctcga tgtattcaag attatcgcaa
    21781 tgatttaact ttttttacaa atgggcttag tgtttgttta gcagagctaa aaggatatca
    21841 ggttaccact ggcgaacctg tttgccagac ccgtcgtcta aatagtcgga tagataaagt
    21901 aagatatatg ttcaataaaa taacttagtg cgttttatgt tgttatataa atatggtttc
    21961 ttattaaata agatgaaata ttctttaata tagatttgaa ttaaagtgga aaggaggaga
    22021 ttgttattat aaactacaag tggatattgt gtcctatttg tggaaataaa acaagactac
    22081 gaatacgagt ggatactata cttaaaaatt tccctttata ctgccccaaa tgtaagaacg
    22141 aaactttaat taatgttcaa aaaatgaata taataacaat caaagagcca gacgccaaga
    22201 cgcagagccg ataatttgag aaatgaaact ctcatcttat cggctctttt tgtttatctg
    22261 aattttactg actagccttc aatatttctt gcttcattga taccttttgc tagtgcttcc
    22321 attaaggata gttctttgtc tgtaaagcta tccatgtatt tttctacctg taaccttcga
    22381 gtgcttttaa tcaaattatc tgtaggtaag aaaaattcat caatagatac atggagtaat
    22441 gatacaagat catacagcac ttgtgtactt gggtgttgtc ctttgttttc tatatttgtt
    22501 aaataacgtg ggtcaatttc aatcattgtt cccacttgtt cacgagtgag accccgtttc
    22561 attcgagcct ctttaatcgc taaaccaacg gctctaaaat cgtatttatc ttctttttta
    22621 cgcatactaa atcacctcta tacattttac tgttcctata taaccataaa caggtataga
    22681 aaaacatgtt atatagttta tgagttccta tagaacactg tggaatttat taatgcgatg
    22741 aatccaattt caaatagaaa ggtcgctgat tttatttacc tatacaactt tataaaattt
    22801 agtcatactc ctagaaagta gataaaattg gcgacttaat ggagggaaaa gtcgccatga
    22861 gaaagataat ctgtcatcta gttctcttaa ttaagttctt atcaaaaaaa cttatccacc
    22921 tgtactggat agatatgacc atcaatgtgg ttttgcagat tacatagata gaaaaatcaa
    22981 agcatatgaa cagagaaaac aatctgttta tgtgcttttt tggtcactta ataaatatct
    23041 tttaggattt ctcttcattt atgcaacaaa tccccctttc gtgtcggggt ttagtatgaa
    23101 gggagataaa gaagcacatg ccactccctt tcacgacaga aagggggtga aaagcatgaa
    23161 accatcttct tttcaagaaa ccatagaaaa tcagtttgac tatatctgca aacaagttat
    23221 tgaagatgaa cgaaaagatt acttcaaaca tctagacaga ctatcagcaa aagaagtctc
    23281 gttttctgat atagataact accttgtcaa ctctttttct actgttgata cgtacgtcac
    23341 tgatttccaa ctatttaaat tatttggatt aagaatcaat gttgaaagtg atttattaag
    23401 tgaagccctg cgtcatcttt cagagaaaaa acggaacatt attttacttc attactttat
    23461 ggatatgagt gatgtggaga ttgcggagtt actaaatttg aatcgttcga ctgtttatcg
    23521 tcatcgaata agtggactag ccatgattaa agaattcatg aaggagtgta aagaatgaaa
    23581 acagcttatc caagggttcc cttccccctc attgtaaagg cgacagatgg cgacgtggaa
    23641 gcaattaatc agattgttaa acattatcga gggtatacct caaaacgttc attacgtcgc
    23701 atgacagacg aatatggaaa ttctcatatg gttattgatg aaaccctacg tggcagaatg
    23761 gaaacgaaac tcatctctaa gattttatcg ttcgaaatta aatagtccca ttttttcgtg
    23821 gaagcgtgtc gaatcgtatc cacgcttccc gatttgggag gtttgttatt ccatcaaagc
    23881 atattgtgtg ttcagtaggt tttgataggc taacaagcca aacgttcttt gacaaccaaa
    23941 taaaggcaat cgaatacgtt tcggtaagaa aagagctgat gaactaacac gccatgacct
    24001 acaaaattag tgagcgattc atgttagtaa ttcgaaagta atctttagca ggattgctgc
    24061 catgactttc ttatcgtgat aatgatactc ccgtatagcc atagtccgag cgttagaagc
    24121 gtcacaggca atgggtacgg ctacatgaga accatgcaga agtgaaattc ctgtgagctt
    24181 tgctagagct gttcgattgc taagatattc ttctcttgtc agtgtattat tataaaacta
    24241 tttgatataa ttataggtga atattggagt tttcctagcc tataattggg aaaggagtaa
    24301 taatgataac ggtcacaaaa aaggatttag ttgagctagg atatggaact tctttttcag
    24361 cagacatcat aaaaaaagca aaagaattga tgattacaaa agggcatatc tactatcaat
    24421 ctcgaaagct agatagagtc cctcgtgaag ctgttgaaga aattctagga attcggtttt
    24481 caaatgaaaa caggtctgag tagtttttgc acagtaagga gtaaaaacta tggttaaaga
    24541 cccaataaaa aaagcaaaga atggaaccta ttattttcgt gcaaatctag gttatgattc
    24601 taaagggaaa aaaattcaaa aatatcgtag tggattcact actaaaaaag aagcaagaga
    24661 agaatattcg aaactgctat tgatgaaacc agaagaatta agtgagaata aggataaaat
    24721 gacgtttaaa cattatatct ttgaaatttt ccttccatgg tataagacac aggtaaagct
    24781 cagaacatat gaaaatcgct taccaactat taagaagcat ttctcttatt ttgataaaat
    24841 ggctgtttcc gatattgagc ctattgatgt gcaaaattgg caactaaaat tatcaaagaa
    24901 gtgtaagtct tcttatgtaa gagctgttca aggactattt tcagttgcta tggatcgagc
    24961 aattgtcctt gggataacca ccacaaatcc atctaagatt attggcaatg tgaaaaagca
    25021 aaagtctaaa attgaatttt ggacaaaaga agaatttgaa aaagtaatct ctctaattta
    25081 caaagaagat tactatcaac actttttatt tatctctctg tggtttttat ttatgactgg
    25141 aatgcgaatt ggtgaagcta ctgccattca atgggaagat attgattttg actcaggagt
    25201 actaaccatc aacaaaacac tctattacaa gaatcaatcg aattatagat ttgttgaacc
    25261 aaaaacaaag gcgagcgttc gtcatattgt attagataaa tgcacattaa cttatttatc
    25321 cgaatggaag gaagtacaac aaaatctaat tcagacagac tttgtaatga gctataacgg
    25381 tattcctacc caaaaacata cactatcata tgctattgaa cgctatgcta agctggctgg
    25441 tatccatcga atcagaatcc atgggttacg gcattctcat gcttccttgt taatcagtat
    25501 gggagaaaat ccacttatca ttaaagatcg tctaggacac gaagatattg agacgacttt
    25561 aggaacgtat ggacatcttt atcctaacag taatttcgag gtagctaaca aattaaatgg
    25621 agccatttcc tttaaagaag caaaacaaaa ttatgatact tcaccaaaga atcaatttac
    25681 tgtagactac ttgcgaaaca aagaaatgaa aaaagtgcaa taaaagtgca gtaaaaagat
    25741 aagaagagcc agaaaccctt ggtataagag gatttctagc tctcctacaa
//
LOCUS       DAYLZO010000026        25646 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.026, whole genome shotgun sequence.
ACCESSION   DAYLZO010000026 DAYLZO010000000
VERSION     DAYLZO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.026"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            133..3660
                     /locus_tag="ACGVJG_001003"
     CDS             133..3660
                     /locus_tag="ACGVJG_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFJ6556976.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACGVJG_001004"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACGVJG_001004"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFJ6556977.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACGVJG_001005"
     CDS             7448..7789
                     /locus_tag="ACGVJG_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFJ6556978.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACGVJG_001006"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACGVJG_001006"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFJ6556979.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACGVJG_001007"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACGVJG_001007"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFJ6556980.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACGVJG_001008"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACGVJG_001008"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFJ6556981.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACGVJG_001009"
     CDS             13000..13737
                     /locus_tag="ACGVJG_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFJ6556982.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACGVJG_001010"
     CDS             13751..14566
                     /locus_tag="ACGVJG_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFJ6556983.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACGVJG_001011"
     CDS             14579..15226
                     /locus_tag="ACGVJG_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFJ6556984.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACGVJG_001012"
     CDS             15242..15898
                     /locus_tag="ACGVJG_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFJ6556985.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACGVJG_001013"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACGVJG_001013"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFJ6556986.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACGVJG_001014"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACGVJG_001014"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFJ6556987.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKEQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACGVJG_001015"
     CDS             18240..18758
                     /locus_tag="ACGVJG_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFJ6556988.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACGVJG_001016"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACGVJG_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFJ6556989.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACGVJG_001017"
     CDS             complement(19645..20565)
                     /locus_tag="ACGVJG_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFJ6556990.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACGVJG_001018"
     CDS             20691..21743
                     /locus_tag="ACGVJG_001018"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFJ6556991.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACGVJG_001019"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACGVJG_001019"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFJ6556992.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACGVJG_001020"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACGVJG_001020"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFJ6556993.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACGVJG_001021"
     CDS             complement(23383..24144)
                     /locus_tag="ACGVJG_001021"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFJ6556994.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACGVJG_001022"
     CDS             24333..25427
                     /locus_tag="ACGVJG_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFJ6556995.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>25646)
                     /locus_tag="ACGVJG_001023"
     CDS             complement(25513..>25646)
                     /locus_tag="ACGVJG_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFJ6556996.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8492 a         4442 c         5409 g         7303 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaaggaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcactt
//
LOCUS       DAYLZO010000027        25309 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.027, whole genome shotgun sequence.
ACCESSION   DAYLZO010000027 DAYLZO010000000
VERSION     DAYLZO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.027"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            221..3610
                     /locus_tag="ACGVJG_001024"
     CDS             221..3610
                     /locus_tag="ACGVJG_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFJ6556997.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3613..5034
                     /locus_tag="ACGVJG_001025"
     CDS             3613..5034
                     /locus_tag="ACGVJG_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFJ6556998.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            5031..6908
                     /locus_tag="ACGVJG_001026"
     CDS             5031..6908
                     /locus_tag="ACGVJG_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFJ6556999.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            7000..7842
                     /locus_tag="ACGVJG_001027"
     CDS             7000..7842
                     /locus_tag="ACGVJG_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFJ6557000.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7994..8869)
                     /locus_tag="ACGVJG_001028"
     CDS             complement(7994..8869)
                     /locus_tag="ACGVJG_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFJ6557001.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            9076..10107
                     /locus_tag="ACGVJG_001029"
     CDS             9076..10107
                     /locus_tag="ACGVJG_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFJ6557002.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10196..10501
                     /locus_tag="ACGVJG_001030"
     CDS             10196..10501
                     /locus_tag="ACGVJG_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFJ6557003.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10680..12695
                     /locus_tag="ACGVJG_001031"
     CDS             10680..12695
                     /locus_tag="ACGVJG_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFJ6557004.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12697..12981
                     /locus_tag="ACGVJG_001032"
     CDS             12697..12981
                     /locus_tag="ACGVJG_001032"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFJ6557005.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            13007..14380
                     /locus_tag="ACGVJG_001033"
     CDS             13007..14380
                     /locus_tag="ACGVJG_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFJ6557006.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14463..16478)
                     /locus_tag="ACGVJG_001034"
     CDS             complement(14463..16478)
                     /locus_tag="ACGVJG_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFJ6557007.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16500..17714)
                     /locus_tag="ACGVJG_001035"
     CDS             complement(16500..17714)
                     /locus_tag="ACGVJG_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFJ6557008.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17935..18879
                     /locus_tag="ACGVJG_001036"
     CDS             17935..18879
                     /locus_tag="ACGVJG_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFJ6557009.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19175..20119
                     /locus_tag="ACGVJG_001037"
     CDS             19175..20119
                     /locus_tag="ACGVJG_001037"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFJ6557010.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(20786..21184)
                     /locus_tag="ACGVJG_001038"
     CDS             complement(20786..21184)
                     /locus_tag="ACGVJG_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFJ6557011.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(21181..21417)
                     /locus_tag="ACGVJG_001039"
     CDS             complement(21181..21417)
                     /locus_tag="ACGVJG_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFJ6557012.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(21577..21861)
                     /locus_tag="ACGVJG_001040"
     CDS             complement(21577..21861)
                     /locus_tag="ACGVJG_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557013.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            complement(22032..24260)
                     /gene="topB"
                     /locus_tag="ACGVJG_001041"
     CDS             complement(22032..24260)
                     /gene="topB"
                     /locus_tag="ACGVJG_001041"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFJ6557014.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            complement(24434..24844)
                     /locus_tag="ACGVJG_001042"
     CDS             complement(24434..24844)
                     /locus_tag="ACGVJG_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFJ6557015.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            complement(24961..>25309)
                     /locus_tag="ACGVJG_001043"
     CDS             complement(24961..>25309)
                     /locus_tag="ACGVJG_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFJ6557016.1"
                     /translation="LLQYARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYA
                     AGKALGYTSTDVTAWFLTKNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDN
                     QMNQSAITDFLNK"
BASE COUNT         8321 a         4729 c         4663 g         7596 t
ORIGIN      
        1 tttttctccc tcaacccttt tctttcaatg aaacgctcat taatcgaagg aatcaacttc
       61 ataaatgaaa ccaattggta tgaaaattgg tgtgattccc tttagcgccc ttcgtataat
      121 aattatcata ttaaaataaa taaaattatg taacgcctgt ttattttaat aattttcact
      181 attcacgtca aaacagaaaa aaagaaaaag gaggggaaaa atgacgacca cagggaagaa
      241 actgaaagtt attttcatgc tgataatatt gagtttatca aactttgtgc cattatctgc
      301 aatagcagac actacagatg atccaacagt tttagaaaca atttcagctg aagtcatttc
      361 ggatcagtct ggaaaaaaag cactgaacat caagctaaat gcgaataaca ccagtgctga
      421 aaagatagaa aaagaaattg gtctagtcga aaattactta agtgatgtgg aaagaaaaga
      481 aggagatggc tatgcttatc aggtaaatag cgggaaaatt acgttggaaa tctcatcaaa
      541 cactaaacaa actatcgatc tgagttttcc aatcgatcca gcactttacc acagccaggc
      601 aaacaagctg atcgtcgata ataaagaata tgacattatt gatgagacag aaaataagaa
      661 agatacagat gtgtcagtac caaagccaga cgaaatagaa gaagaatcat caaaagaaaa
      721 cgaaaattct gtcagcccat ttacattgcc tacattatcc ttgccagctg tgagtgtgcc
      781 atctaatcaa acgattccta cagaatatac aacagatgat cagggcactt atcctaaagc
      841 cagttggcaa cctacaggaa atacaaatgt tcttgatcat caaggcaata aaaacggaac
      901 aaatcaatgg gatggtataa attcttggaa tggagatcct aatgatcgga cccattcgta
      961 tatcgaatat ggaggaaccg gtaatcaagc agactatgcg atacgaaagt atgcaaagga
     1021 aacaagtaca cccggattgt ttgatgttta tttgaatgct cgtggaaatg tacaaaaaga
     1081 tatcacgcct cttgatctcg tattggtcgt agactggtca ggaagtatga acgacaataa
     1141 tcggatcggt gaagtaaaga ttggtgtcga tcgttttgtc gatactttag cagatagcgg
     1201 tatcacagac aaaatcaata tgggatatgt cggctactca agcgaaggat atagctacag
     1261 taacggtgca gtacagatgg gttcatttga ttcagtgaaa aatcaagtaa aatccattac
     1321 accttcacgg acaaatggtg gtacttttac acaaaaagca ctaagagatg caggaagcat
     1381 gctatccgtt ccaaatggac ataaaaaagt gatcgttttg ctgacggatg gtgtaccaac
     1441 attttcctat aaagtacagc gggtacacgc acaatcaagc agcaattatt acggaactca
     1501 gttttctaat acgcaagatc ggccgggaaa tacttctcta atctcaagaa tctatgatgc
     1561 acctgaccaa aacaatctat ccagaagaat cgacagtacg tttatcgcaa ccatcggaga
     1621 agcgatggca ctcaaagaac gaggaatcga aatacatggt cttggcatcc aacttcaaag
     1681 cgatccggca gctggtctct caaaagcaga agtagagtct cgtatgcgac aaatggtttc
     1741 atcagatgaa aaaggcgatc tttactatga atcagctgat catgcaacag atatctctga
     1801 atacctagcc aaaaaagctg tacagatctc agcaactgta agcaatggac aaataaatga
     1861 tccaatcgca gaaccattca tttatcagcc tggtacactt tcagtcaaga gtgtggggac
     1921 aagtcctaca acggtcactc catctatttc catagaagga aataccatca agagcaatca
     1981 gatctattta ggaaaagacc aagaaatcca aatccattac caagtgagaa tccaaacaga
     2041 aaatgaggac ttccatccaa atttctggta tcaaatgaac ggcaggacaa ctttccagcc
     2101 aaacattgat accaatgaat tagctgaatt cggtatacca tctgctaaag ctcccggagt
     2161 cagtcttcac atcaaaaagt tatgggaaga atttgacaac aatctagctg atcgtccaga
     2221 tcaagttact tttgagattc aacgggaaca tacgacaaat gctgcagctt ggaaaaacgg
     2281 atatattcga atcattaaac cagctaaaga tacaacaaat acgtgggaac gtgcagacat
     2341 tgacaaatta tctgcaaata gcggagaaag ttatcaagag atattatcac tacctcaata
     2401 caataatcaa ggtcaagcat tcagttacca aacaatcaaa gaattacctg taccaggata
     2461 cgattctcaa caaatagatg caatgacatg gaaaaatact aaacaattca caccgttaaa
     2521 cttgaaaata acgaaaaatt cctctacagg tgaaaaggat cttattggcg ctgttttcaa
     2581 attaacagga gattctattg atactttact aacagatcat ggcgacggaa cctattctct
     2641 tccagaaaat gtcaaattgc aaaaagaaat gacctatacg ctgacagaaa caaaagctcc
     2701 agaagggcat ggattaagca aaaagactac ttgggaaatc aagatcgctt ctgatggtac
     2761 ggtaaccatt gatggaaaaa cagtcactac ttccgatgat acgatccagt tgactattga
     2821 aaatcctttt gttgaagttc ctgtagcagt acgtaagtat gcgatgcaag ggacggacaa
     2881 agagataaat cttaaaggag cagcattttc cctacagaaa aaagaagcaa atggtactta
     2941 tcagccaatt gacagccaaa caacgaatga aaaaggtctt gccagttttg attcactcac
     3001 acctggtaaa tatcgagtcg ttgaaacagc tggtcctgcc ggatatgata cttcgccggg
     3061 aaattatgaa ttccaaatcg ataaatatgg aaaaatcatt tacacgggaa aaaataccga
     3121 gatgacaaat aatgtatgga cgctcactca tcaaaatcga ctaaaagcgt ttgatctaac
     3181 ggtacacaaa aaagaagaca acggacagac attaaaagga gcaaaattca gactgcaggg
     3241 accagaaatg gacttagaat cgccaaaaga tggacaagaa acagatacct ttctattcga
     3301 aaatttaaaa cctggaactt atacgctgac cgaaactttt acaccagaag gataccaagg
     3361 tctaaaagag ccagttacta tagttataca cgaagatggg tcaattcaag tggatggaca
     3421 agatcatgaa tctgttctgt caccaggagc caaaaacaac cagatttctt tagacatcac
     3481 gaatcaggca aaagtaccat tacctgaaac gggaggaatt ggccgtttag gaatctatct
     3541 agtagggatg attggttgtg cgttttctat ttggtatctt tttttgaaaa aagaaagagg
     3601 gggcagctaa atatgaaaaa acttggttgg cttagtatgt gtctcttctt gttactattt
     3661 aaaccagctt ttactcaggt agcaacagaa acagaaacag aaatggttca gattacttta
     3721 cacaaattgc ttttcccaaa cgggcaactg ccgaaaaatc atccaaatga cggacaagaa
     3781 aaagctttat tacaaacgta tcgaggatta aatggtgtca cattccaagt ttatgatgtc
     3841 acagattctt tttaccatct acgggaaaag ggcaaaacgg tagaagaagc acaagcagag
     3901 atcgcaaaaa acggtgcgtc ttccggtatg tttaccgcag aagcaacaac tacaactctt
     3961 aacaacgaag atggtatcgc ttctttttct ctggccgcta aagatcaaga aaaaagagat
     4021 aaagcgtatc ttttcattga atccaaagta ccagaagtcg tcaaagaaaa ggcagagaat
     4081 atggtagttg ttcttcctgt acatggacaa aacaatcaaa aactttcaac tatccatttg
     4141 tatcctaaaa atgaagaaaa cgactaccct gatccacctt ttgagaaggt attagaagag
     4201 cctagaaatg attttacgat tggtgaaaaa atcacttatt ccttgcatac gacaattcct
     4261 gtaaatatcc ttgactatca aaagttcgaa ttgtcagata gtgcggatga agcattaacg
     4321 tttttaccta atagtttaac gatttcatcg aatggagaaa agctgacaga aggctttgtc
     4381 atacacaaga aacctcacgg atttgatgtt ttattttcga tcccttcgtt ggaaaaatat
     4441 gctggaaaaa aactgaccat ttcttatcag atgcagctaa gcagtacagc acaggcgaac
     4501 aaggaaatca acaacaacgg aacactggat tttggttttg gtgtcagtac aaagaaagtc
     4561 tctgtatata cagggagtaa gcaatttgtc aaaatcgaga caaataaacc agataaacga
     4621 ttagctggcg cagtattcct tattaaaaac aaagcaggaa attacctcca gcaaacagcc
     4681 aacggataca agtggacaaa gaacgaatca gatgcgcttc acctgatttc cgataaaaat
     4741 ggcgcttttt caatttccgg gttgaaaaca ggaagttatc gattaaaaga gatcgaagca
     4801 ccttctggtt atattttaag tgaaacagaa attccgttta ccatttcaac ttttctttct
     4861 gaggataaag aggcggacag tatattgaaa gtagtcaata aaaaagaaaa tagccgtcca
     4921 tttcttccaa aaacaaacga aacgaaaaat acacttttag gcgttgttgg tatggtattc
     4981 gcaagctttg caatctggtt gtttatcaaa aaaagaacag gagtgaaaaa atgaaaaatc
     5041 ataaaaaaat aaacgttatg ttaggagtcc ttttccttat tttaccatta ctcacaaaca
     5101 gcttcggcgc aaaaaaagtg tttgcagagg agacagcagc tcaagtcatc cttcataaaa
     5161 agaaaatgac tgatttaccc gatcctttaa tccaaaacag cgggaaagaa atgagcgaat
     5221 tcgatcaata ccaaggatta gccgatattt cattttcagt ttataacgtc actcaagaat
     5281 tttatgcgca acgagataaa ggagcgtccg tggatgcagc aaaacaagca gtccagtctt
     5341 tgactcctgg tacaccagtt gcttcaggaa cgacagatgc tgatggaaat gtcactttat
     5401 ctttacctaa aaaacaaaat gggaaagatg cagtctacac gatcaaagaa gaaccaaaag
     5461 acggagtgtc agctgccgca aacatggttt tagctttccc tgtatatgag atgatcaaac
     5521 aagcagatgg ctcttataaa tacgggacag aagaactaga tactatccat ctctacccta
     5581 aaaatacagt cggtaatgat ggaacgttga aagttacaaa aatcggtact gccgaaaacg
     5641 aagcactaaa tggagcagaa tttattattt ctaaagaaga aggaacacca agcgtcaaaa
     5701 aatacatcca aagtgtcaca gatggattgt acacttggac aactgatcaa accaaagcca
     5761 aacatttcat tactggtcat tcttatgaca tcggcaacaa tgactttgcc gaggcatcta
     5821 ttgaaaaagg ccagttgatc gttaatcatt tagaagttgg aaaatataat ttagaagaag
     5881 taaaagctcc tgataatgcg gaaatgattg aaaagcaaac aatcacgcct tttgagatcc
     5941 tggcaaatag ccaaacacca gtagaaaaga ccatcaaaaa tgatacgtct aaagttgata
     6001 aaacaacacc tcaattgaat ggaaaagatg tcgcaatcgg tgaaaaaatt caatatgaga
     6061 tttctgtcaa tatcccatta ggtatcgctg ataaagaagg aacgcaaaac aagtacacaa
     6121 cattcaaact tatcgatact catgacgctg ctttaacatt tgataatgat tcttcaggaa
     6181 cgtatgctta tgccttatat gatggaaata aagaaatcga cccagtaaat tattctgtca
     6241 ctgagcaaac agacggattc acggtttcag ttgatccgaa ttatattcct tcattaactc
     6301 ctggcggtac attgaaattc gtttactata tgcatttgaa cgaaaaagca gatccaacca
     6361 aaggattttc taaccaagca aatgtcgata acgggcatac aaatgatcaa acaccaccgt
     6421 cagtcgatgt cgttactggg ggcaaacgat ttgttaaagt agatggtgac gttacatcag
     6481 accaaacact tgctggagca gaattcgtcg ttcgtgatca agatagtgac acagcgaaat
     6541 atttatcgat cgacccatcc acaaaagccg tcagctgggt atcggcgaaa gaatcagcaa
     6601 cggtttttac aaccacaagt aacggtttaa tcgatgtgac aggtctaaaa tatggcacgt
     6661 actatctgga agaaacgaaa gcgccagaaa aatatgttcc attaacaaac cgtgtagcat
     6721 ttactatcga tgaacaatct tatgtaacag caggacagtt gatttctcct gaaaaaatac
     6781 caaataaaca caaaggtaca cttccttcaa caggcggtaa gggaatctat gtgtatatcg
     6841 gtgcaggagt agtccttcta ctgattgctg gactgtactt tgctagacgc aagcacagtc
     6901 agatttagtc tttttgcaag aattggaaaa aggagcagcg gattacgcaa atgctccttt
     6961 ttcttttatt atcaaacagt catcacaagg agaacacaaa tgaaaccaaa gaaaaggaaa
     7021 cttctagatc tgctgatgct tttcttgctt ctctcaggaa tagggatact agcttatcca
     7081 tttgttagcg acgcgctgaa taattatctg gatcagcaga tcatctctca ttatcagcaa
     7141 caagcggtca aagaaaatga agaagtcatg gctaaaatcc aagaaaacat gacaaagaaa
     7201 aaccagcaat tagcaaaaga aggtggaaat ccaggtgcag atccctttac taagaaaaaa
     7261 gaacctgcga aaaaggatag aacctatttt gagaaacata cgatcggtat tttgacgatc
     7321 cctgagatca atgtccattt gccaattttt gatgaaacga atactgtgct gctagaaaaa
     7381 ggtgcctcat tattggaagg aacgtcttat cctacggggg gagaaaatac acatgccgtt
     7441 atttccagcc atcgaggact gcctcaagca aagttgttca cagacttacc gaaactgact
     7501 ttaggtgatt ctttctatat cgaaatcaat ggacggacac tcgcttatca agtcgatcag
     7561 atcaagacgg tagaaccgac tgatacaaaa gatctgagaa tcgaaaaagg gcaggatttc
     7621 gtgacactcc taacttgtac accttatatg gtcaacagtc atcgcttact tgtcagaggg
     7681 caccgggttc cttatcatgc aaaagaagtt caaaaagaag tacagaaagt atccgagaac
     7741 aaacgatttt tcctgtatgg attgttagct gcatgtatac tgattttcct ccttcttata
     7801 tatctttttc gtcaatcgtt caaaataaga aacagaagtt agctattcct catttctata
     7861 atatatctat tcgaatcgtt ccgtaaacaa acaggctgag gtttgaaaaa gaaaacttca
     7921 gcctgttttt atgatgatat caaagagagc ctatcaaaca ctatctttct tattactaaa
     7981 tgaggatctt ttcttaagtt ttccgatatt ctttaagaag gcctttttgc atcaattctt
     8041 gtttgttttt caagttgcgg atattctctt caaaattttg agaatatatc actgagaata
     8101 acgtatttaa tacgtaatcg aaggcaattt gagaggagaa agtcgctact ttagcaaaat
     8161 catattcctc ctgcgatata acaagacaca tatcagcctg tttggccatt tctgaatgct
     8221 gatttccggt aatcagcaag gtaggggcct ctacatgctt caaataggtc attattcgtt
     8281 cataatgatg gatgcgaccg ctgtagctaa taaaaatggc gcaatcctcg ctagtcaaat
     8341 tggctgcatt ccaagaagaa tcactgtatt cttcagccat gatcaaaaac ttattcaact
     8401 tgaccatttt attctggaat ttccttgccg tgatttgaga atcacctttt gcaaaaagaa
     8461 agattcgctg tgccttgttc aatactttac cgatttgatc taaatcttca tgttcaatct
     8521 gcaattgtgc tttttttatt gactggatcg ataaatccgc cattttcttt gcaatttgct
     8581 gagtgctatc ttcacttgaa aaaggaaaat taggatcaat cgtttgattg aactgagcaa
     8641 tcaattgatg aacttcccgc cccagctcga aacggaattc tttaaatccg ctgaatccca
     8701 tttttttgca taaacgaatg atagctgaat gggaagtata cgtagctttt gctaattctt
     8761 caatcgtcat gtagactgca gcttcgatat tcctacggat gtaatcgaca atccgtttct
     8821 ctgtcgtggt aaacaactct tgttttgtta gtttttcttc aataagcatg atctgacctc
     8881 caaattctat cttactgata aacacttggt tccgctacta tctttttgat ttttatcctt
     8941 actttgaaca ttaatttcac caaaccgaat gataacaagt gtttttctga acttttttac
     9001 cagaatagct attataaata ctttctgcgc ttttctagga tatactgaaa acatcaataa
     9061 aaggaggcac cactcatgct tacaatcgct tatcttggca atggaaagag tacaaatcgc
     9121 tatcatttgc ctttttcaag aaaacttacg aacaaaatca atatcaaaaa aattttttcc
     9181 ccatctgaac cagtgtggaa aacattcgaa gacatcgaat atactgatca gctgtctgat
     9241 ctttgggatg atcctgagat ccaattagtt gtcatcacta cacctagtca atttcattat
     9301 gaatatggaa aaatggcact cgaacatggt aagaatgtac tagtagagaa accttttgca
     9361 gaaacgtcaa aggaagcaga agagttattt gcattagcaa aagaaaaagg tctatttatc
     9421 caatgttacc aaaacagacg gttcgattct gacttcttga cagtccaaaa agtaatcgaa
     9481 agcggaaaat taggggatat tttagaagta gaaatgcatt atgattattt cagaccggaa
     9541 gtaccagaaa atacgcatga attttctact gcgaacagct atttatatgg acatgcttgt
     9601 catacagtag atcaagttct ttcttatttc ggcgaaccag atgatatcag ctatgatgta
     9661 cgtcaacttt tagggaaagg acgaatgaat gattacttcg atctggattt tttctatgga
     9721 aatatgaaaa tttcgataaa atccagttat ttccgcatca aagaacgtcc aagtttcgtc
     9781 gtttacggaa aaaaagggat gttcgtgaaa caaacaaaag atcgacaaga agaacatctg
     9841 aaaatgtttt acatgcctga tcatgaagac tttggtatcg atttgcccga acattatggt
     9901 gtattgacct atatagatga acaagggagt tatcatgaag aaaaagtaat ttccgaggtt
     9961 ggtgattaca gccgtgtcta cgaagggatt tacgagacat tagttaatgg cgcagaaaaa
    10021 gtcatcaaag atgaagaaac aatcttacaa atgaaaattt tggaagaagg aatcaaaagc
    10081 attcaagaat cagaaggaaa aatatgacat atttgtaaga atcgggtttg ttttttgacg
    10141 cataaactgt cttattgtac gatgaaaaag tatagaacta ctaggaggaa ccaacatgac
    10201 aaattatgac ggtgaaaaac acaaacgatt aagaggttca atggccccta aaacagaaga
    10261 agaagtggct ttagcaggaa ctaattctgg tgaaacagaa gacaaagaag aatcattagc
    10321 tggcacaaac tctggtgaaa cagaaaataa agaaacagct ttatctggtt caaattctgt
    10381 agaagaggaa acaaagccta atgagcccaa tcactttgaa aaagatatcg aaaaagcggt
    10441 tgaagatgtc aaagaaaaat ttgaagaact gacctcaaaa aataaacatg atcatcattg
    10501 atcggacgaa atcaattcat aaaagcagga gacatttggt cacctgcttt ttttctgtat
    10561 ccattcgata gaaatcgttc ggtacagtcg taaaaaaaat ccaactgtaa gttgtaccaa
    10621 acacccctcg atttaaaagc gctaccatac tagaatataa gtaggaggtg aacagcgctg
    10681 tgaactacaa aattagttat ctcctgaaaa caccaagaga aatcagtcta gaaaaagcag
    10741 cagagatcat cgaagtaaat acaagcgagg cgatcaaaca attaactcct tttgccgaat
    10801 atgtggatga tacccgtatc cgtataaaga aattccgagc aatcgactgg gcagaaaaat
    10861 ttttggatat tactcgattt gaagctgtgt ataccgaaca agagcgaaga gatcttcttt
    10921 atttgatggt ttattcgact gcaacgtctg tttctgtttt tcattttcaa gattttcttc
    10981 aggtttctaa agggacagtc ctgtcagata tcaaaaagat aagaaatgaa ttagaagtgg
    11041 aagattgtca gctgtcgtat actaggaaaa ctggttttag gctgctaggg tcagaagcaa
    11101 tattaagaag agaagcaaaa aatcgcactg ctcgtttgat gcagtcactt gctggaagat
    11161 ttgggctcac gtatttaacc gcaacccttt ccattgatta ttacgccaaa gttagagatt
    11221 tgctagataa aacgatttca ctatcagatc ttcgattcgc accaaatcga atggatgaat
    11281 tagcttatta tttgagtttg acaaccattc gaattggaaa aacatccatg gaactggtaa
    11341 aagatgctga actgttggct tctcttgcag tcactgacat tagtcgacaa gtattgactg
    11401 gccttggcta ttccgaacat caaaattcgg agattatatt tttctctctt tgcttagcat
    11461 cgatcgttga aggagatgtc caggagcctg cacttgattt tttgctggaa tgttcttcag
    11521 aagtcatcca tcgcatggaa ttcctgatgg ccgtagaatt tgcttcgttt cgtgaattat
    11581 tgatgaatgt cttttatcac ttggtccctg cctattttcg tatttcctat tctttttatt
    11641 tacctaatgt gatgatcgat caaattaagc atcaatatgc ttccatctac gaaatgacaa
    11701 gaaaagcatt acttccgtta gaaagacgaa tagggaagag catccctgaa gaagagattg
    11761 gttttttcac tatactattt ggcggagaga tcagaaaagc ggacgcagaa gaaagaaata
    11821 gaaaaataag agccgtgatc gtctgtccaa gtggcattag ctcttcattg atcttaaaaa
    11881 gtgaacttca gcaattattc ccgatgatct tttttacaga gactaattca gctgaccgct
    11941 tacacgaggt tgacgaaact tcttacgata ttattttttc tactgtacct ctcacactta
    12001 ctgataagaa aaagtctctc tatgttctgc atccaatcat gactgacttg gaaaaaaatc
    12061 agctgatcaa tcaagtacaa agagattggc tgataccagg attttcaatg ccttctgccc
    12121 aagaattgtt gtcggcgcta ctgccttata tcgaattaaa agaaggcgta gacgaagaaa
    12181 agctctatca agtattaaat cgtaaaatga acaaactttt agaaaagaag gaggatcaac
    12241 gtcctatgct atctgaatta ttaacagaag aaatgatcca attatcggat caaccaaaaa
    12301 actggcaaga agcaatcaca tttgccgcac agccgctatt atcagaaaat aaaatagaag
    12361 aaagttatat caaagctatg atcgagcgcg tggagcagta tggtgcgttt atccatattg
    12421 gcgatcacat cgcattacct catgctcgtc cagaagatgg cgtaaacgaa gtagggatgt
    12481 ctttgctgaa acttcaacaa cctgtggatc tgcttgataa tccagcccat cccattagtt
    12541 tgttcatctg tttagctgct atcgataatg aggcacatct tcgggctttg gcaaatttaa
    12601 caaagctact ttctaataaa caaaatcttc aagatttatt acacgcatca acaaaagaag
    12661 aaatattagc gatcataaga aaaggagacg aatgatatga aaatttttgc agtatgtcaa
    12721 agtggattag gaacaagttt tatggtagag atgaacatca aacaagcact tgcagatctt
    12781 ggagcagatg tggacgagtt tgaagtaagt catacggatg ttggaagtgt atctagcgat
    12841 atggctgatt atttcttcat tgagcattct ttgaaagata cgctaacttc tatacctgat
    12901 gaaaagctcg tacctttgca atcgatcatt gacagcgaag aagtaaaaga aaaagtaacg
    12961 aagatcttgt caaaagaata aaaatgattc aagggaggaa ctatcaatgg ataaattgtt
    13021 gaatttgttt gtgtcaatcg tcagtcagcc ctccatcctc gtttctttga ttgcactgct
    13081 tggtctcgtt cttcagaaga agaaattttc agaagtgatc caaggaacta tcaaaacatt
    13141 tgtcggattc ctagtactga tcggcggtgc tggtttaatc agtaattcac taaccccttt
    13201 tgcttcgatg ttccaaacag ctctgcatac tcaaggagta gttcctagca atgaagcagt
    13261 agtggcgatt gctttgcaac aatacggtac acctactgct ttgatcatgc tagtagggat
    13321 gatcgtgaat attttacttg ctagattcac aagatataaa ttcatttttc ttacaggtca
    13381 agcaatgctt tatgtttctt gtctaacagc agtcatttta gtaagcgcgg ggtttactgc
    13441 aaatttgtgg atgatcttat taggcggggt cttcgaagga acactattaa ctattacgcc
    13501 tgctctttgt caaccgttta tgaagaacat cactggaaac aatcaagttg cgttagggca
    13561 taccggaaat atcggttatg cttctagtgg atggatcgga aagttttttg gaaacaaaga
    13621 ccgttctaca gaggacataa agattcctaa aagtttcagc tttttgcgag attctactgt
    13681 atcgatcatg ctcttgatgt cgatcgtata tgtcgcactt gctttgttag ctggtcctgg
    13741 atttgttgaa agagaactaa gcaatggaga caacgctgta atctttgcac tgatccaagc
    13801 tggaacattc acagctgggt ttgttatcgt ccttcaaggg gttcgaatgg tgttggcaga
    13861 aatcgttcca gcattccaag ggattgctaa aaaactcgta cccgattcta aaccagcatt
    13921 agatgttcct atcgtttttc catatgcacc taatgctgta ttaattggct tttttgttag
    13981 cttctttgta ggaacactat ccatgtttgc tatgataggc ttgcaaacta cagttatcat
    14041 tcctggggta gtaggacatt tcttctgcgg agctgcttct ggtgtcttcg gcaactcgac
    14101 tggcggtaga agaggggcgg tattaggttc tgctttcaat agcttattga ttagttggct
    14161 gccattattt atccttccag tattaggaga tcttaaaatc gcttcttcta catttgccga
    14221 tactgattat ctagtaccag gacttgtact tgggaaacta ggagaatacg gtcaaatagc
    14281 cttgacagtt ggaatcatcg ctttcttttt acttgtcgta ttatctagct ttctttttgc
    14341 cggaaaaaga acaaacattg tttctaaaga acagaattaa gcaaacttca aaataaaaat
    14401 tgcttggaca atatcagaag aagatttaaa gtcttctgat atgtcgcaag cagttttttg
    14461 ttttatgcat ttatttttga atagcttgtt aagacaagtg gctgttttgc tagaagagga
    14521 taacttttac cattcatttt cactgttccg cctgttctat cagaagtaat cacgatctta
    14581 tcttttgttt tttcaagcga aatctttcca tctttgatag gtagcgtaca attcagtgtt
    14641 tcaaatgaat ctagattagg ttgtatcata aattcttcat aacctggctt tgttggatac
    14701 aaaccgataa aatagcgtcc taaaagatag attgggcttg ctccccatgc atggcataag
    14761 ctttttccat aaggatctcc atacatactg tacttttcat ttcctgtttg agtagggtcg
    14821 tattcttccc agaaagttac cgcatcatga gataacatcc ctccccaata atcaagaatc
    14881 gtttggtaga cgatattcgt ctgtccgagt ttgcataaag catcttgttc atagaatttg
    14941 aaatatggag tagtaatctg tgtgatagtg tcattcaata agacattctt taagatcgat
    15001 tgctgttggt ctttgttagc aaaatcgaat aaaacagcga aaatattcgc atgacgcgtc
    15061 acatttcttc tcccagattc atagcaatcg ataaaagcgc caagttcttc atcccaaaag
    15121 tagtcaaata ggttttgttt caatagttga tatttttctt gatagagatg agtatgataa
    15181 ttttcgttct ctccatatgt ttcagctaag atatctccgc aagtgatcat ggaatgatag
    15241 gctttcagta ataagacttg ttctgcagca agcgttcctt ctttatccat ttcagaccaa
    15301 tcaataaata tccaatcttt tgctctccca taaataaacc ctaattcatt cgtttgctcc
    15361 atcaagtatc tcattaatga ttgcatctta gggtagacga tgcgtaaaaa ttcatagtct
    15421 ttcgtcatca tatagtggtt ttcgatacca atcaaccaca agatcgaata gtcaacgata
    15481 gtattcatat gctgcttgat ttcattttgt cctcttaatg ctaatatcgt tcgtttattg
    15541 atttcctcgt caaaaaaaag atattgattg atcatgaatg attgatacgc atctccagac
    15601 caaatccagc gatctcgttt gataccgtcg ataaaaaaca aatcactaca taacttgaag
    15661 gcttcttctg atactgacca gatctgattc agtaaagaag aatcgcttga aaaagcagct
    15721 tgtttcgaaa gagataaaaa ttcatgaaaa acttgaagtt gaaccattcc agtagaaacc
    15781 tctggtataa acaaatagcg aaaagcgcgt ttacgagctt tcatttttct tgtcattcct
    15841 atttgcttat agtagcaata atcgatatcc atagcttcta catcggactc tccataacat
    15901 agagtgatag attcgatatg gtcatccaga gatattagtt ctacacagcc attcactgct
    15961 cgcccaaaat cgaataagat tcctccgcca atctccgttt cagtgactgg atggacgcat
    16021 tctttttgat aatagatttg atttggatct tttgcgatat cgacataaag cggactccag
    16081 ccagctggat attcttcaat aaaattactt gctgtccagc caatatctga ttttacttcg
    16141 tctccctcga taaacatcgc tggtagccct gatgtatgtc ctacaaagat acgaattttt
    16201 gctttaccag ctggacaagt cagccatttt ccacatggat gtttttgtcc attaatttct
    16261 acatacccaa ctccttggat cgtcacttta aacatggaag gttgatcaag aaagtaatat
    16321 ctcttaaact tgacgttccg atggcaatca tccatgtacc agtacgctgg ccagtcaaaa
    16381 cctcgttctt cccgttggaa attttgtagc agtccatggc ggatctcaaa gtctccagga
    16441 taccaaagcc atctatcttt atgtttttca tttgtcattt tacacgacct ccatcaaatt
    16501 tattttgttg tcaattgttc ttttatttct tcatctactc ttatcactgt ctttctattc
    16561 gtcctgtgta ctactagaag gctaataagc gcgctaatca acagcaagat actgccattg
    16621 acgaaaacag caagtgtttt actcccaaga gaagcaaaag agagaaagag caaaccatat
    16681 ggtgcaatga aagtacctaa attagcgcca accaataaga ttgaagtatt gaaactctcc
    16741 gcgtacttgg gcgcttggat acttacttgg ttgaataagt aaggaatgaa taacgaaaaa
    16801 gaaattcctg ttaggatcgc accaaaccca atcacataca ggtttatcac agtggcaatc
    16861 aatacaaagc cgattcccat cagtagtaaa gaaagtgtaa gcatatagcg atgtgctttt
    16921 gtctgaagcc agccaaaaca aactccggca gccatacttg ccaattgcat gattgttaag
    16981 atattcgctg cattagtttc gtttcctaga gaataagtcg tcaggactgt ggctaattta
    17041 atcgatacag cattatatac cacgatgacc agcaagagaa aaataccata actaatcatt
    17101 ttaggattcg ttttgattac ttgtcttgtt ggggctgtgc catgcgattg aagttcagtt
    17161 gtttcaggtg taggaacaaa caaaacaaac aagacaagaa taggtaagct gatggcgtag
    17221 actaaaaagg tatattgcca accgaactgt actagctgtc ctgcaacaaa tgtcattaaa
    17281 gctgcaccaa atccttgaaa tgcactttga aaaccaatca tagaggctcg ttcatctccg
    17341 tgataaaagg cagcaatcat gctgactgcc aaagaattga tcaatccaaa gccaatacct
    17401 agtccagctc ttgaagctaa gacaatactg taactttgtg agaagagggg gaggatacca
    17461 caaatcgatg caatcactaa tccgatgatc acagtttgtt tttgtccgat tttcctagca
    17521 ataaaactac taatcaaaac aaaaatagtg accataaaag agggagcagt cgttaataat
    17581 tcaacagcag ataaagaacg cgtggcataa gtagctgaca tactgggaat tgcagacgtg
    17641 actgcaccag cagaagtcaa aataagagag actgacagca gagaaaattt catcatcttt
    17701 ttattcataa gcatgttcct ttcatttttg taagcgctta tcaaaaatat agtatgaagt
    17761 ggttattctc tcaatagcgg aatcacagga agttcatgtc ccaaccatag aaagtcttta
    17821 tgaaaccaaa aatagctttg catataatag cagtacagcg tgaactaata agcgaaagaa
    17881 accgcttaca aaaaggaggg agtcaattgg aactagctga attagacatt ttttttgcgt
    17941 aacgaagaat caatcgaaac tctgcagcgt gaacaaggaa tgaatatcaa tgatttaaac
    18001 ttagcttacc atcttcagac accgccaaaa atccaccgag gggatctatt tcaacaggga
    18061 aatattcata tcagtaaaca ccgtcgatat gcggaagttc ccgagcatac acatgatttt
    18121 gttgaaatca attatatgta tagcggtaca tgtttacaaa ttattaatgg agaaaggata
    18181 gagatggaag aaagtgacgt ggtgatcatt gataaagaag ctactcaaag aatcgaatac
    18241 acagggcaag aggatatttt aatcaacatc ttattaaaag aagaaacctt atctacagag
    18301 atattgaatc atttggcaag cacaaataat ctactttctt cttttttgat aaatgcatca
    18361 aaagaaaaag gggaacatac acaattcatt cattttcgcg gcggaaaaac aagtcgtttg
    18421 acgcatttga ttgaaaatat gctgttgatt tattttgata catacagtca taagatccgt
    18481 tcgttaaatc tctatacatc actattactg atcgaattga ctcatttatt agaacaacaa
    18541 agcttttcat cagcagatgg atgcgcagaa aatgagatgc ttagagtatt agattatatc
    18601 gataagcatt ttacttcttg tacgttaaaa gaaacagcag ctcattttgg ctacaatccg
    18661 gtctatctta gtaataaatt gaaaatgtat tttgatgctt cctttcaaga actggttcta
    18721 aagaaaaaat tcgactttgc gttggaactg attcaagaaa cgagtttttc gttggaagaa
    18781 atcgctaatc gtatcggata taaacaaacg acttctttat tcaaattatt caagaagtat
    18841 tcaacatgta ctcccaatga atggagaaat aaacattaag aagaaaatct ttcactaaag
    18901 catatgaagg aaaattgttt taggtgcgat tgctgtctta ttatatcgtt gcagcgaatt
    18961 tttttggatt tattttcagt tctaaacttt tggttgtatt atcaggggat ataggtgtaa
    19021 acatttgtca tttatctgtt atgcgtattc aggaattcgt ttccatcaaa aattataata
    19081 acaactatta taattaacat gttgataaaa tgaataaaca agactatact tttcatgaaa
    19141 gtaacaaata gttatgaaaa ggagctgttt attcatgaaa aaaacaagtc gtaaagtagt
    19201 aatcgtcggt acaggttttg ttggaacaag tatcgcttat gcaatgatca accaaggaat
    19261 ctctaatgaa ttagtattga tcgatgtgaa tcaagaaaaa gcagaagggg aagcattgga
    19321 ccttttagat ggtatggctt ggggcgatga aaatgtggct gtatggtcag gtggctatga
    19381 agaatgtaaa gatgctgata tcgttgttat cacagctggt atcaaccaaa aacctggtca
    19441 atctcgttta gatttagtta aaacaaatgc gtcaatcatg cgccaaatcg taaaagaaat
    19501 catgggatca ggattcgacg gtattatcgt tgttgcttca aacccagtag atattttgac
    19561 atatatcgct tggaacgaat ctggtctgcc aacatcacgt gttatcggta ctggaacaac
    19621 attggataca actcgtttcc gtaaagaaat cgcattgaaa ttaaaagttg acccacgcag
    19681 cgtccacggc tatatcttag gtgaacatgg tgattcagaa gttgctgcat ggtcacatac
    19741 aactgtcggt ggtaaaccag tgtttgaaat tgtagaaaaa gaccatcgta ttgcccaaga
    19801 tgaattagat gtgattgctg ataaagtccg taatgctgct tatgagatca ttgatcgtaa
    19861 aaaagcaact tactatggta ttggtatgag tactgcacgt atcgtaaaag ctatcttgaa
    19921 taacgaacaa gctgtattgc ctgtatctgc ttatttaact ggtgaatacg acgaaaaaga
    19981 tatctttaca ggtgtgccat caatcgttga cgaaaatggt gttcgtgaag ttgttgaatt
    20041 atcaatcaac gaagaagaaa aagcaatgtt cagcaaatca acaagtgctt taagagaagt
    20101 attgaacact gtattataag aaacaatgaa tatgaactaa aaatcctaaa actgcttaac
    20161 agctgaaata gctgttaagc agttttttat tgatatctaa taggtaagca aataaataga
    20221 atggttaaaa ttaatcggtc cgaaaatgta tccgtacata tgaacatatg aaatttaatc
    20281 tgttgattac aataagcgaa tcgttatcaa tgtttatctg tggttaatta aaaaatcctt
    20341 ccgcaacggc aaatacaatg attcatatct aattcgttca tatttgatta actgaataaa
    20401 gaaactggtt tttatcattc aattaaacga caaaaaaagt aagaaaaact gcaaaatttt
    20461 tttataattt tgcataagca gattttgatg gaaaaatgac gatttttgat aaaaaaccgt
    20521 tcgagagagg tttatcattt tttaaatcta ctttgtataa tatatattat gtaaactaaa
    20581 atatataaaa aatcagccca ctagttctaa accactattt cctactattc actcttttgt
    20641 attcctttta aatcaattat ttcaaaatta atccatattt tccatatttt tatgaaaaaa
    20701 ctattttgta tgattaaaca taaaaaaatc agcatattcc ttaatgacat gctgatctaa
    20761 ttgagaaata aagtttttaa ttcgttcatt ttattgcggt ttgtcgtaaa taactggtta
    20821 tcttggactt tagtgaatcg aaatccaatg aactagtggt aatcattatg ataaaatcaa
    20881 cagcttcttg acgagaaaaa gcaactggac aattattcaa ttgaagaaac aacaacatgg
    20941 caacaagagc agttcgttta ttgccattat gaaaaggatg ttttttagct aaattaattg
    21001 ctaaaatcgc cgctttttca tagatttcag gatagagttc ttgattgaat actgcttgtg
    21061 aaggctgttt gacggccata tctaatgcat ttgcatcttt tacaccgatc atttcgcctg
    21121 gcgagtgacg taaaataact ttcgcgttga ttttgataat ttctttagtc gaaagatacc
    21181 tcataaatca accaaatctt ttaacgcgtc ttcatattga ttcatgatcg aatcaactaa
    21241 tgaatcaata tcaggttctt cttgtgcttt gatcattcgt acagaaccat cagattgaaa
    21301 aacataactg attgcgtcac ctgtctgcac acccatgaat tcttttactt ctggtgggat
    21361 agttgttaca tcgctattgc cagattttct aattttatac tttttcattt ttttcatatt
    21421 tcgccaccct ctataccatt ctcattctat agtaagtaca ttatatccaa gtatatactt
    21481 ttaatcaatg tatatacttt tattttgctt tttcttacga aaaaatgaca aagaaaaaag
    21541 tttgtgacat gacttttgtc acaaactctc gtattcttaa ccaaaatatt gatgaaatac
    21601 ttgtgtaata actggtagaa aataaataat gatcatagcc gctaccacca ccaacaattg
    21661 ttttcgctgt ttctttcgct ttagcatgct tgtcgtttta tgaaaaaaat aagtagcgct
    21721 tttagctcct gcagtcaaag tcgataaaat caacgttggt gaatatgctg tttgattgtg
    21781 cgtcaatgac aacaacttcg tcccgtaatc aggatacttt agaaatgctt ggtacacagc
    21841 cacaatcgtg attgctccca taagataatt taaaaatgaa tgcacttttt tcaaataaaa
    21901 aaactccttg ctatctatat aaaatcagcc taccccaacg tttttgcttg attcactgat
    21961 attttttagt tttattggta tttatattct cacttttcgg aataaatatt ccgctatttc
    22021 attgttttca tttactctac ttcgttttca ttattttttc ttgatgtttc tttgtttcca
    22081 tcaactgctt ttgttccact gttttcgatc catggaaaag aaaaaccgat tgtcatatct
    22141 tcttttaata ctaaaactgc atcgaatttc ccttttttac cagtaaaacc tttgacaact
    22201 ttacttgtct ttccttttga aagcaaatct ctgagattcg ttttagtcag cttcttttta
    22261 gctactgttg gccacaattt gaagtcgcat tccggattca agcatgtagc gattttagga
    22321 taaagcatga ctattccctt tttacatttt ggacatcttc ctaattgatc gtttttcttc
    22381 tccatcgctt ttatctgacg tgtcccgcta atctcatctg aaaagtctac cttgtcgatt
    22441 tgattaggaa cttctgccaa taaatgtagg atgaattttt tgatattctc taaaaaaaca
    22501 gatggtgtcc cctcttgttt accaattttt ctcaagtacc cttcccagcg cgcagtcatt
    22561 tctgcgctcg tcagtaatgg ttcattggct actgccttac aaagagtgat tccttttggc
    22621 gtaacagtta tttcgttttt attgacttcg atgtaatgct tctgcttaag attttcaatg
    22681 atattggctc gtgtagcttc tgtccctatt ccttctactt ctttgagaat ttcttgcgct
    22741 tcatcattat ccagcgtttt tcccgcagtt ttcattgctg tgatcaacgt tccttcggta
    22801 tagggttttg gaggttgtgt ttctttctcc atacttttca gttctacttc tgcttcttca
    22861 ttttctacga taggaggaag agtctgagct tctttctttt ttgacttgat gcgtaagatt
    22921 tcctgccaac cttgctgtaa tgggatcttt ccgatgcttt ttagctttaa ttgctgtacg
    22981 cccgtcatta cggtcgtctc ttcgtagata taatctgcag caaacatagc cacagtagtg
    23041 cgcaaaactt gcaaatagat tgcttgctgt aatttggaaa gcttcgcaaa gcgtgcagga
    23101 gtcggaatcg tctttgtcgg aataattgca tgatgttctt gaactttttt cgcatcgaca
    23161 tatcgtttct ttgctttgat ctcagttgtc tgtacttctt ctattcctaa aaatttttta
    23221 taattttgca gatgtttaac cagatactga tgctcatttt ctgtaatgta aaaggaatcc
    23281 gttcttggat agcttagaaa cttgctttcg tacaatcctt gagcagcatc aagtgtagct
    23341 ttggcacttg ctttcattag ctgattcatc tttgtctgca aactagatag cgaaaataaa
    23401 cgtggactat gcaacctctt ttcctttttc gtaacttcaa gaatccgtcc gttttgctta
    23461 cctaaatgcg ctccaagttt ttctatttcc ttcgttaact cttcttgggt tttaaatgct
    23521 ttttttggta cgatttttcc agaaaactgc cctttgtttg tcgtaattaa accttctcct
    23581 tcaaagtacg tttctttttt gaagtgtttg atttcttctt gcagctgata aatcatatag
    23641 agtgtcggtg tttgtacacg accaagagaa aaactgccat caatcccttt tcgttgaagt
    23701 agcaagctat ataatggact tccattcatt ccaatcagcc aatcagcgtt ctgccttgct
    23761 tgcgcttctt tatattttgg aaaatagttc tttccaggtt ggagattctt aaaaccttca
    23821 tagattgctt ctttttcaag agaattgatc cataaacgta aatagttctt ttccttagaa
    23881 aatgcgtttg cttgtcgaat aatcgaccac gcgatggctt caccttctcg atcaccatct
    23941 gttgcaataa tgatggtatc ggctgactgc aattctcgtt ttacgatcga aaattgtgca
    24001 cgtttgtcca ttggaacttc gtaacggaag gtttctggga aaataggcaa attttctaaa
    24061 gaccattttg cccaacgctc ttcataagct cctggagcaa ccatatctac caaatgtcca
    24121 aaaccataag tgatggttac ttcaccagca aacaatggat cgctgatttc ataataccct
    24181 tcatgccgct tttgctgttg gaaggtttcg gcataggctt ttgcttgtga aggtttttca
    24241 gctagtatca ccgtcttcat cttctgacct ccgttactta attttttgcg gacttctcta
    24301 ttatggaaca tattttcgca tctggcaata aaaaaaagat accgtatagt cgaaatagac
    24361 acaaaaacat caagcaaaca aacgatgatc cgtttgctgc ttgatgtttc cagaaaatat
    24421 tcatctttag ctgtcactct tggttaatct gccagttgat gccaaatcga tcagttacca
    24481 tcccatacaa ctgagcccac ggaacagcat ccagtggcat ctcaatctta ccaccctcag
    24541 ctaagctttc aaataggcgc ttcgtttctt cttcatttct tgtctggatg actaaagaaa
    24601 cattatttcc cacaactaat tcaggaccaa ttccttctgg cgtatctgca aacataacta
    24661 atgtatcata aatgttcagc cgtgcattcg tgattagatt ttttgcttct tctggcaagt
    24721 tcatttgttc tattccaggc atttcaccgt atgtggtgat acctgatgct tcagtctgaa
    24781 aaactttttc ataaaaagcg atggcttcac gcgactcatt tttgaagttc agatagatat
    24841 ccaatgtaaa cagctcctct cttggttaaa tctaccataa gaaagcgttt tttacgagtg
    24901 aatcgtttga ttgattcatg acaaaagcct tgcgcagctt cttcttgatc ttttttatga
    24961 tcatttattc aaaaaatctg taatagcaga ttgattcatt tgattatcaa tttcaagaat
    25021 actgccagca taatcacttt gcccataatt ccacgaaccg tctactggca cacttattcg
    25081 atcaatgcca ccagctcctc gtgcaatcga aaagaggttt ttcgtcaaga accaagcagt
    25141 gacatctgtg gatgtatatc ctaaagcttt accagctgca taaggtgcac ggatcagatt
    25201 caatggattc ttcaattggc tgaaaatagc gttcattact tgctgttggc gtctgacacg
    25261 gccaaagtct ccttgttcat ccattcggaa acgtgcgtac tgcaaaaga
//
LOCUS       DAYLZO010000028        25269 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.028, whole genome shotgun sequence.
ACCESSION   DAYLZO010000028 DAYLZO010000000
VERSION     DAYLZO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.028"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..1047)
                     /locus_tag="ACGVJG_001044"
     CDS             complement(<1..1047)
                     /locus_tag="ACGVJG_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFJ6557017.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTN"
     gene            complement(1293..2414)
                     /gene="mnmA"
                     /locus_tag="ACGVJG_001045"
     CDS             complement(1293..2414)
                     /gene="mnmA"
                     /locus_tag="ACGVJG_001045"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFJ6557018.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            2818..3417
                     /locus_tag="ACGVJG_001046"
     CDS             2818..3417
                     /locus_tag="ACGVJG_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFJ6557019.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(3555..4841)
                     /gene="efmA"
                     /locus_tag="ACGVJG_001047"
     CDS             complement(3555..4841)
                     /gene="efmA"
                     /locus_tag="ACGVJG_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFJ6557020.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(5112..5456)
                     /locus_tag="ACGVJG_001048"
     CDS             complement(5112..5456)
                     /locus_tag="ACGVJG_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFJ6557021.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(5593..6738)
                     /locus_tag="ACGVJG_001049"
     CDS             complement(5593..6738)
                     /locus_tag="ACGVJG_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFJ6557022.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(6918..7892)
                     /locus_tag="ACGVJG_001050"
     CDS             complement(6918..7892)
                     /locus_tag="ACGVJG_001050"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFJ6557023.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(7972..8847)
                     /locus_tag="ACGVJG_001051"
     CDS             complement(7972..8847)
                     /locus_tag="ACGVJG_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFJ6557024.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            8977..10884
                     /locus_tag="ACGVJG_001052"
     CDS             8977..10884
                     /locus_tag="ACGVJG_001052"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFJ6557025.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(11425..14703)
                     /locus_tag="ACGVJG_001053"
     CDS             complement(11425..14703)
                     /locus_tag="ACGVJG_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFJ6557026.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(14974..15519)
                     /locus_tag="ACGVJG_001054"
     CDS             complement(14974..15519)
                     /locus_tag="ACGVJG_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFJ6557027.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(15531..16223)
                     /locus_tag="ACGVJG_001055"
     CDS             complement(15531..16223)
                     /locus_tag="ACGVJG_001055"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFJ6557028.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(16242..16526)
                     /gene="macP"
                     /locus_tag="ACGVJG_001056"
     CDS             complement(16242..16526)
                     /gene="macP"
                     /locus_tag="ACGVJG_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFJ6557029.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(16539..17102)
                     /locus_tag="ACGVJG_001057"
     CDS             complement(16539..17102)
                     /locus_tag="ACGVJG_001057"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFJ6557030.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            17330..17992
                     /locus_tag="ACGVJG_001058"
     CDS             17330..17992
                     /locus_tag="ACGVJG_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFJ6557031.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            18116..19312
                     /locus_tag="ACGVJG_001059"
     CDS             18116..19312
                     /locus_tag="ACGVJG_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFJ6557032.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(19413..20789)
                     /locus_tag="ACGVJG_001060"
     CDS             complement(19413..20789)
                     /locus_tag="ACGVJG_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFJ6557033.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(20826..21692)
                     /locus_tag="ACGVJG_001061"
     CDS             complement(20826..21692)
                     /locus_tag="ACGVJG_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFJ6557034.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(21781..22806)
                     /locus_tag="ACGVJG_001062"
     CDS             complement(21781..22806)
                     /locus_tag="ACGVJG_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFJ6557035.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(22824..23498)
                     /locus_tag="ACGVJG_001063"
     CDS             complement(22824..23498)
                     /locus_tag="ACGVJG_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFJ6557036.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(23682..24575)
                     /locus_tag="ACGVJG_001064"
     CDS             complement(23682..24575)
                     /locus_tag="ACGVJG_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFJ6557037.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(24649..>25269)
                     /locus_tag="ACGVJG_001065"
     CDS             complement(24649..>25269)
                     /locus_tag="ACGVJG_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFJ6557038.1"
                     /translation="ELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEKVTTGGRKFEKY
                     DASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWATSGQATEFVADG
                     NGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEEIQIVGVENPGP
                     ERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         7478 a         5465 c         4444 g         7882 t
ORIGIN      
        1 attagtcaag attgcagtaa caactaacga ataagttggg gcattttttt tattcgtttt
       61 tccccaacct ttcggcaaca gaccgtctcg agccatcaac atcgttgttt ctgctgggag
      121 catcgtccaa gacaaccaag aaccaatgat cgaaataatc aagccgatat tgatcagcgc
      181 tgctccccaa tttcccacga ttccttttaa aatatttgcc atagcaggtt gtgaaatagt
      241 tgctaactct tcttgagata agacgccgta tggcaagatc gaagcaagga tatagataaa
      301 caataaacaa agcagaccaa tgatcgatgc ttgctgggct tctgaacgtt tcttcgcgcg
      361 gttggctaaa acacttgcgc cttcgatccc tacaaagacc cacatcataa ccatcaaaca
      421 acctttaatc tgcaaccaga tcgttccttg ctgccctgtt ccattcacta gattatcaga
      481 tacgttaccc caaaaatcag cagtgaatat attcacttta aatgcgataa atacaaatat
      541 taaaaatagg aataatggca ctaatttaca gatcgtcacg attgtattga tgacgctcgc
      601 tccttcaact ccattgttta ctaataaagt cagaccccaa ataaaaatac ttgcaaataa
      661 aatagaagga acattttgtc cacctttgaa tatcgggaaa aattctccca tggcgctcat
      721 cagcatcgtt gcaaaagcaa catttcccag ccacgcagac aaccagtaac cccaaccact
      781 gatgaagcca cctaatttac caaatgaagc ttccgcgtaa ccgaaaatac caccatctag
      841 atctggacgc ttttctccta aatgattcag cgataatacc aaagctaaaa taccaatccc
      901 aacgattatc catgaaagga tcgctggccc tggagctgca gaactagcca gatcacttgt
      961 gatcccaaac acaccgcttc cgattgacga actgacaacc agagcaacca gtccccattt
     1021 gctaataccc gtcgatttct tttccatttc catcaccctc acttataaaa ttgaacaaat
     1081 cacttgcaaa agattccctt ctattttgca actgctcttg cttaacaaaa agcaacttta
     1141 ttcatattat tggatattat gaacttttta cacaagtatt tcaagtataa aatgaataaa
     1201 aattaatttt attgcaaaaa aacagctatg ctatccaaaa gaaaaggtgt ttcttttgtt
     1261 agccatagct gttttgattc atttaatatc gcttaaacgt attgtaaaac ttttgtttct
     1321 tgatatgcgt ggtcgatcaa accaccaccc agacattcca ttccatcata aaatacaact
     1381 gcttgtccag gagtgattgc acgtacaggt tcgtcgaata cgacttctgc acgattatca
     1441 tccaataagc gaactgtcac aggaacgtct tgctgacgat aacggaattt cgctgtacac
     1501 ttgaattctt ttggcattgg ttcattcgtc gtaaagtgga tttcacttgc atccaagctt
     1561 gtggcataca atgcaggatg atggaaccct tgccccacat ataatgtgtt tgtcgctaga
     1621 tcttttccta caacaaacca gggttcttgc gaatctcctc cgccaccgat ccctagtcct
     1681 tgacgttgac caatcgtata atacatcaag ccagcatgtt gtcctttgac ttcaccatca
     1741 agagtgacca tatttccttt ttttgctggc agatagttgc tcaaaaattg tttgaagttc
     1801 ttttcaccaa taaagcagat tcccgttgaa tcttttttct tagcagtcgc aaggcctgcg
     1861 cgttcagcaa ttgcccgtac ttctgatttt tccatgccgc ctagagggaa catcgttttt
     1921 gaaagctgtt cttgtgataa ttggctaagg aagtaagttt gatctttatt attgtctact
     1981 ccacgaagca tgtggaccac gccgttttca tcgcgagtga cttgcgcata atgtccagta
     2041 gctacataat cggcgccaag atccattgca taatccaaga atgctttgaa tttgatttct
     2101 ttgttgcaca tcacgtctgg gttaggtgta cgtcctgcgc ggtattcggc taggaaatat
     2161 tcaaatacac gatcccaata ttctttttca aaattcacag agtaataagg aatcccgatt
     2221 tgagcagcta ctttcgctac atccttataa tcttccgtgg ccgtgcatac accgttttcg
     2281 tccgtgtcat cccaattttt catgaatatt ccgatcacgt cgtatccttg ttcctttaac
     2341 aaaagtgctg ttacagatga gtcgacaccg ccgctcatcc ctacgacaac gcgtgttttg
     2401 ctgttgtctg tcatgatatc accatcattt caattagatt ctgaaacatc tacgagttga
     2461 aggtcgcaac ttttcagtat tttacattat acgtcatatt tctgaaaatt tcatccctta
     2521 attatcaagt cgtaaaaata ctgttctact ggacctattc agtacacaaa catgatagtc
     2581 aaccaaaaaa tcactcatct tttgagcatt cgatataaaa aattggcata ctcataaaaa
     2641 gggccagcct ttcggcaata aggaataatt ttataaattg tgaaaaacta tttttcaaaa
     2701 ttgcttctta tcgcttaaag ctgcacgtct catttacaac cttttctttt tagatgttat
     2761 gagttgctca gataagtgat tttgtttact ctaagaaaaa agggaggcaa aagatacatg
     2821 aattacttta tcagtgatat gcactttttc catgaacgct tattaggaaa aaacgacttt
     2881 gctccgcggc tctttgcaaa tgtggatgaa atgaaccaac agttgatcac tagctggaat
     2941 gcagtaataa aagaaaccga ccatgtttat catctaggcg accttgctat gcatccgcaa
     3001 tatgaaaaag caaatgcaga aattttggaa ctggtcactc aactgcaagg aacaatccac
     3061 ttgatcaaag ggaaccatga cagtcgcgct tttttccgtt atttagaaaa aaatgatcct
     3121 aggcttactg acggaagtcc caaattttat ttttatgatg taggtgccat cgttaaattc
     3181 gaccatcaac aatattactt gactcattat ccgatgttgc tcgggcctaa cgacactatc
     3241 cgcaatcttc atggacatat ccatcattac agcgtaccta tcgagaacga tgtcaatgta
     3301 ggcgtagacg caccggaaag agaactctta aaggaaaaac taccatttgg cactccgtta
     3361 tctgagcaac aaatcaaaga aatctatgaa gcaaagagga agtacgatag gtcgtgaaaa
     3421 aactgcccta tatcaagtat taaacaaata aagataatta ctcatatttt tcatttgagt
     3481 aggcttattg ccacaggaac attaggatca gaaatggagt tgtaacaaga cttctgtcac
     3541 aactccattc catactattc gtctttttgc tcatggtgtt cttttaccag ttttttctgt
     3601 aattctttat cataatttcg ggcagctgac gttaaaaata aaacgattcc gcataataaa
     3661 atcccgattc cggaaaacaa gaacattttt tctaccccta aacgatcagc taatggtcct
     3721 gcaaataata atccaattgg acctgccagg ttcatcaaag agttgaagtt ccctaagaca
     3781 cgacctaaaa cattcgattc ataactctgc tggatcattg ccatcagcag tgtattgaaa
     3841 tatggcgttg cacaacctgc tcctgcattc aagataagaa agtacaagaa cccttgacta
     3901 tcgtttggaa ccaatccgct tgcaccgatc gtcaggccca taatgatgta ggctataata
     3961 ataggtttca ttcggtcttt ccatgttcct ataaaactga tcagtgcacc gcctaagagc
     4021 attccgactg cgtagactac ttccactgct cccgcctgtc caacggaacc accaaagtaa
     4081 tctagcgtca tcaatggata cagactgatt gctggcatga acaacagcat cacaaatgca
     4141 ccattgatcg ttatgtacca taacccttta ttatccatca attgctgcag cccaaatttt
     4201 gcatctttaa aaatagtaag cggaacttcc aatatttcag gagaaacttt tgggatcttc
     4261 acaaaaatca ataagccgac accaaaaact gcccccaata catctaataa aataaggtaa
     4321 ttgacaggaa cgactgaaaa caaagccgca cctagagctg gcgcaatgat gaaattcgct
     4381 gattggacca ttcctaactg tccattcgtc cttgttatgt gagaagatgg aacgattgtc
     4441 ggcataatcg actggatcgt tggcatttgg aatgtttgtg cgaccgaacg cataaacaaa
     4501 gacacaaaaa ctagccaaat cgggaaggat tctgaaatcg ttccgactac tgctaaaata
     4561 agcgcgaaaa tcgctacaat aatatccgtg acaatcaaaa gagctttttt atcccagcga
     4621 tccactagcg gtcctacaaa tgggcttagt aaaaccatgg gaatcattcc tagtaaagta
     4681 gcaaaactca aaattgtcgc cgaaccggtt tctcttgtca aataccagat gatcgcatat
     4741 tggacgacca tactagtgat tcctgacaaa aactgtcccg ataaaaagaa caaataattt
     4801 ctcttccaat tcgttaaaac gaccgactgt tcattttcca taaacatccc tcttttcttt
     4861 ttcattttgt atagcgtttg cacaacaatc attatacggg attctttttt caaaaaatac
     4921 ctttttccct aaataacaat cgtttcaatt taaatttctt tacttagaaa caataaatca
     4981 gcaaaatatt aaaaataaca acatgtgtta aatttaaaaa taaaaaagac aactaatttg
     5041 cgtcgtcctt acaaagttag tcatctaaca ataggccgtt tcaaaactag ttgtcttttt
     5101 atatccacag atcattcgac tttttcgaat aacccacggc taatcatccc atccatcaga
     5161 aaataaaatt tatcttggct cattttctgt tttggatctt tgaaaatatt gactgctttc
     5221 aatccatttg ctgtagtgat cgacattttc aatgtttgaa tatcttttga tacagtgatt
     5281 tttaatttga atccttcttt gctttgcggc gtggcatcta atggtcggat caattgataa
     5341 ttgcctccgt ttgtttctgt aaatccatta tcgcgcaatg tatatctttt tgcatcagga
     5401 tgcaaacgat agctaattgg tgcgcccagt accgttgctg ttgtttcaaa tgccatgatt
     5461 ttcacctcga taaaattagt ttctctttat tataaaagtt tatcctgatt tttactaggt
     5521 ttttcagtta agttccctaa aaaatgaagg cgttctctca gacatctgtt catataagaa
     5581 tgacttcact agttattttt tgacagcacg aactaattga tcaaggaaat actggatgtc
     5641 gtcttcggta tttcccaaac caaagctgat acggatggat tcttttagta tcggtgtatc
     5701 ttctccatac attgcttcta atacgtgtga aggctcgata tttcctgctg tacaagctga
     5761 accagtagaa atagcgaccc cattaagatc aagcttcatc agtagcaaat catttgcttc
     5821 gccaattaag cgaagattca acacatgagg gagtttattc gttaaatcac catttacttg
     5881 ataagggatt tcgtttgctt ctaatgtatc aagaatcaat tgctggaatc ctttatattt
     5941 gttgttacgt gcttcacgtt cttcaggtga cagcagttca actgcttttg ccatacccca
     6001 gatccctgct aaattttctg tacctgcacg ccgcttttct tcctgctccc cgcctaaaag
     6061 caatgcaggg atatttactc cgtctcgttt atataagaaa ccgacgcctt ttggcccatt
     6121 gattttatga gcagagatac ttaataaatc gattcctaat tcttctggat ggatcgtttg
     6181 gtttccgtac gcttgtaccg catctgtatg gaaataagct ggatgatccg ctaaaagttc
     6241 accgatttct ttgattggca tcaaatttcc gatttcattg ttcccgtaca tcacagagac
     6301 caagatcgta tcttctcgta acgctttttt gacctcttcg atcgacagat tccctttttc
     6361 atctacaggc agatatgtaa cgtcaaaccc ctttgtctct aaatatttca tggtattaag
     6421 aacagcagga tgttcgatag ctgtagtgat tagatgtctg ccttcttttt gacgcgaaaa
     6481 cgctgtttct aagatagctg tattatcccc ttctgttccc ccactgttaa agatgatctc
     6541 gtgaggtttt gcttgcagac tgtctgcaat gatttgtctt gctgtttcca atttttcatg
     6601 cgcgtggcga ccaaatccat gtatgcttga aggatttcca aacgtgtctt gcatgattgc
     6661 cgtcatcgct tcaattactt gtggatgcat gggggtagtt gccgcatgat ccagataaat
     6721 agttttcact tcattcatcc cttccaaatt attgctaggc acattctagc ataaaatagc
     6781 gaacaaacca aagaagagct catttttgtc tagaggacgt ttcatgggca tagaaaaaag
     6841 gagctgtgac aaaagtcttg tcgcagctcc ttgaagctgc tcacttctgt cacaacttct
     6901 ttccctcttt tttttgttta ttcgaattct ttttcttcca gacggaataa cggactcatc
     6961 ggtgtatttt catggatgcg tttgaccgct tcacccatta attctgaaca tgtaacgatt
     7021 gttaagtttt ctggatgacg attttcttct gtgaaaacag aatcagtgat acaaatatct
     7081 ttgattggtg cattgtctaa aatacttttc gcatgatcgg acaataatcc atgtgacgca
     7141 caggcatata cttcttttgc gccgttttct tttaatacat ttgctgctcg tgcaagcgtt
     7201 gctcctgtgt tcaagatatc atctaccatg atacatgtct ttccttgaac atcaccgatc
     7261 acataaccac cctcagttga agcatcatct gcatgatcta cgatggctaa tgtgctgttc
     7321 aaatattctg aaagactacg tgcacgctga acaccgctgt tttttggcga aacaactacg
     7381 atgtcatctc ccgtcagacc taattgacgg taataatgag caaacaacgg catcgtaaat
     7441 aaattatcta caggaatatc aaagaatcct tgtacttgaa ccgtgtgcag atctaaagtc
     7501 agtactcgag ttgcccctgc ttcttcgatc aagttagcga tcaatttcgc agtgatcggt
     7561 tcatgaggtt tcgccgtgcg gtcttgccta gcatacccat agtaaggcaa tacgacattg
     7621 atcgttttcg cacttgctcg cttcattgca tcgatcatga tcagtagttc catatagtaa
     7681 tcattgacag gtgcattcgt tgattgaacg atatacacat gatcccctcg aatactttct
     7741 tcgatattga tggaaatctc gccatcacta aattgcttca cgacgctttt acctaattct
     7801 gttccgaaca ctttcgcgat tttttcagct aatggacggt tgccattcaa actaaaaatc
     7861 ttcaacgtgt catcctgata cttattactc atgtagtcct ccccaaaact tctctattct
     7921 tcgatagttt aacatatttt tccaagattt tctgttaatt tcagcaaatt cttaaggaag
     7981 tgaaactgtt ccgttactcc aactggcagg aatgacttta tgataatcgc cccagttgtc
     8041 gtgtactttg taataacgtt ctcctgtttc agtttcaaaa taagcgtacg ctgtcaccca
     8101 atgattgcca tatgtactgc caagtacttt caaaatacca atcatcacag gtttgccttc
     8161 acgtatacgt tttgtagctc gctgccatga acctagaaga gttgctcgtg cacgctcatg
     8221 attgccattc ttctttaaaa aactagaaat ccctgtacta acctgaaaag gaatcgtagg
     8281 aagtcctagt ggttggatct ggctacgcaa tgccttcgtc aaaaccaacg aatcagccgt
     8341 atttttcttg cgaatctcat ttggaagcat ccacccctta cgatagtctt gataataggc
     8401 aaggagaact gctgccgcgt aagtaccaca aagatagcct gagggggtag cccatttctt
     8461 atacctcaaa aaacgttctt ctggcaatcc cacccagcaa tttgcgagtt cctttcctga
     8521 ccttttttct tcgatcaaaa cgggaatatc ttcttttctg ttgatccatc tagataactc
     8581 gattggctct tcattgatca gcttccattt tttacaagaa agaccctctt gataagtata
     8641 ttttgctgga gaatattcta ccggatgtcc tgattgatcg ataatcacat aactttgatc
     8701 ttcaaaaacc agagctaaaa aaagcaattt actttttctt gactgcagct gctgagtgct
     8761 ggctaatttt ttaatttttt tatctacttt gaaatataaa ggtttatcca ctacttgatt
     8821 cagttgggat cgcctccttt ttttcattat aagtgaagga ttgaaaaaaa caaatcagtt
     8881 tgattgttcg aattttcaat tttttatgtt ctatttgtta attccttcat tttgtaagat
     8941 aatgggcaca cgaaacaaaa aaggagcgat cgatgaatga acaaagaacg attgaaacaa
     9001 gatttctatg aagcagtcaa tgaagaatgg caaaaaacag caaaaatccc tgcagataaa
     9061 ccagctacag gcggtttcca agatttagtt gacgggatcg accaactttt gatgggagaa
     9121 acagacgatc tgcttgccca tccagaaaaa gtgtcttcag aaaaaatgaa acatttcctt
     9181 gcatattatc aactggcaaa cgattatgac cgaagaaatg agttgcatgc tcaaccgctt
     9241 caacctataa tcgaaaaagt tgaatcattg cagtctttcg acgaattgaa taagcaattt
     9301 ccagaatggc tgttggatac attaccattg ccatttaatc tggatgtaga tgcagatatg
     9361 aagaatgcgc aaacgaacgc cttctttgct tatccgcctt ctttattcct tccagataag
     9421 acgtattatg catcagacca tcccaacggt gcacaactaa taggcgtttt ctttgatatg
     9481 gctgttcagt tattagagct tgccggcaaa gaaaaaagtc aggcagaagc aatcacggaa
     9541 caagcagtgc aatttgataa attgattgct cctcatgtaa aaagtgcaga agaaaacgca
     9601 gattacagca aaatgtataa tccgcaatct tttgatacat ttattaaata cacagataaa
     9661 ctggacttga aaaaacttgt cacgggtttg attgggacaa ctccagataa agtgatcgta
     9721 actgatccag tgtacttcga tcatttgaat gacatcctga cagaagctca ttttccgtta
     9781 ttcaaaagct ggatgatcat taaaacagta cgttctctta gtggttatct atcagaagaa
     9841 ttccgccaag tcagcggaat ctattctcgc gtattgtctg gaacagacga agcaatgcct
     9901 cagaaaaaag cagcgtatta ccttgcctct ggacaattcg atcaagtagt tggcgattac
     9961 tacggaaaaa aatattttgg cgaagctgct aaaaaagacg tagaacaaat ggtgaaaaaa
    10021 atgatcgccg tttataaaaa acgtctagaa gaaaatgact ggcttgggga tgcaacaaaa
    10081 gaaaaagccg tcataaaatt aaataactta ggtatccaag taggctaccc cgataagatc
    10141 ccagcattat tcgaccagtt caaaacagta cctgcagatc agggtggtac tttgctttca
    10201 aatactcttg ttttcagcaa actcacatta aaagatcgtt tcagcaaatg gaacaaacca
    10261 gtggatcgtt cagaatggga aatgagtgct gacacggtca atgcttatta tcatccattt
    10321 cggaatgtga tcgtctttcc ggcagcgatc cttcaagcac cattttacag tctagaacaa
    10381 tcaagctctg ctaattatgg tggaatcgga gctgttatcg ctcatgagat ttcccatgca
    10441 tttgataata acggcgcttt atttgacgaa tatggcaact tgaacaactg gtggacagaa
    10501 gaagatctta cacaatttca aaacaaagca caagcaatga tcgaagaatt cgatggtctg
    10561 ccttttgctg acggaaaagt aaacggaaaa ttaaccgttt cggaaaatat tgctgatgca
    10621 ggcggtctaa gctgtgcatt ggaagcagca aaaacggaat cagatctttc tatcgaggaa
    10681 ttctttgtca attgggcaac gatttggcgt acaaaagcaa aaaaagaata ccaacaactt
    10741 ttactgcaaa tcgatgtcca tgcccctgct aaactccgag caaacgtgca accaaaaaac
    10801 cttgacgaat tctatgaaac atttggtatc acggaacaag acgctatgta tctagcgcca
    10861 gaaaaaagag tgcgaatctg gtaagaggtt gtgccagaag tggtcagctt caagaaataa
    10921 gaaggaattt gggaaaatcg cttctcaaat ttttgtgaca tttcggctta tttttcgaag
    10981 aagttacttc tggaatacca tgtattagag gttgtgaaaa agctgcttag tttcaagtag
    11041 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttgac ttactaccga
    11101 gaaactggct tttgaacacc gtttatcgag aggttgtgaa atcagcgtcc tgacctgagt
    11161 attagagaaa aaaacggaaa aatagttcct catattttgc cgtttgttga gctaataccg
    11221 agggtcagct gattgaacac cgtttagtgg agagattgtg aaaaagctgt cctgcatcaa
    11281 gtaataagaa cagccaatca aaagcagcaa ggagaacctc atctataaaa agatagaagg
    11341 aagtgagaca tctattggtc tcatttcctt ctatttttac tttcctcagt caattgtttt
    11401 acatcataaa aggagaaatt ccacttattt ttttcgctgt ttcatgacat aaacaactac
    11461 accgattaac aaaattcctc cagctaatgc tagcaacggg ttattaaatt ctcccgtttt
    11521 tgggagtcct tgagtgccat ttttttcact ttcttttgct gtcctatccg gatgagaagg
    11581 ttttccattt ggttttccat ttggtgattc atttcctgga ttttctcttt ctggtttgtc
    11641 atttcccggg ggcacatcgg cagtttctcg cacagtgatc gctttttgct gggtaacaga
    11701 ttctgtatga gcagaccatt tttccccatc aaatatttcc gaacgataag tgacttctag
    11761 cgtatattct ccttttgaag gtgctgaaaa aatagcggta tgattagaaa attgagcact
    11821 tgactgtgtt tcaacgattt ggatcttttc tgggaaataa cgcatatctt caaagacagg
    11881 cgttccttga tttggtgtga aagttgacac aaaaactgta aaaggcttca gttcaatgat
    11941 atttccttct gcattctccg ccacaccatt tgcttcttgt ataataatcg gattattttg
    12001 ttcatttggt tttgtctgat ttggttcttg tacgattgca tactctggat attccgaaga
    12061 cggtgaggtt tctccgtcaa aaatagcacc tacagataat ggaagaccat ccactctttc
    12121 cttttctgtt tgacgattcc ccggtttaat ctgcatcagt ccatgaacat ggatacttcc
    12181 attttcatag cgcgtaacag gctgctgacc ttctttcgaa catgtattta gagactcgat
    12241 ccaatctttt gatatggtat gtccaagagt attttctgat tgtttctctc gttcattcca
    12301 atagtagttc aacgagtggt agatttgatc ttcgttttga ttcaatgggc ggatctgctc
    12361 tctcatctca agattctctg ttcggaaaga taagatacca gaagcatggt agtctgtgtt
    12421 tcctgctgaa gaggtataaa gagccacatt atatgaaccg ttattaaaac ttgtactgct
    12481 attgacgata atatctgttc cgctattgga atcgattcct ttcgcaagat tttcgaaaga
    12541 caaactattg atcaattcat gagggcccgt taaagaagaa ccgcccattt tgaatccatt
    12601 accgttacct tctccctcaa ttcctggtac ccagccgttt cgataagcaa ctgagttttt
    12661 gatgattact tttccgatcg aacctgtttc atttttagca aaaaggtccc aaccgtcatc
    12721 tgcattatga tacgcgatac aaccgtcaaa tacatttcct tctcctattg tcagtttggc
    12781 cgcaaatcca tcggcatctt caaatcctgg atcggcattt tcaaaagatg tacagttctt
    12841 gactaagtta tgcgcgggcc atgcttcgaa aggatcattt gagctaccgc tgatttggat
    12901 tcctgtattt ccattccgat aagcatgaat ctcttcgatc aaattatgat ttccagaaat
    12961 ctgtaatcct ttttgcatag ctgcactatt tgtaatgtca aaccctcgta aaccccagta
    13021 gtgactgaac agtgtcatcc catttccagt tcctttaaaa tcaagtacag gacgcttgtt
    13081 ttctggattt ttctctgcaa tcaactgaat cgggttttct cttgtcccgt taacgccttt
    13141 tggaatcgtc aatcctcttt cgaaggaata agttccaggc gccatgacga ttgtctgccc
    13201 agcatatgcg tatctcaatg cttgtgtcag agataatgga ttttgtctgc ttccgtttcc
    13261 tgcatctgac ccttctggtg ttacataaac tgtcgaatat ttaggtttac ggtagtccac
    13321 tgtatgaaga atctcttttg tttcataact agtcatctct acgtattctt ctggcggata
    13381 gtttttatct ggcgtaaaac tgaaagtaaa ttcgttcgtt ccttccatca gttttgtctg
    13441 ataatcaaat tcccgattcg cagtgatagc caagtttttt gcgttcttaa gcacctctcc
    13501 tgttgcttta tttttaattg tcaataatcc gtcagcattt gctctaaagg acatagtatg
    13561 atcttggttt cctgaatgat tactagaggt cacagtgtaa atcggatcaa tcaagcgagt
    13621 aggtctttct tgcccaggag gatctgtcgc aggatctgag taagcaactt cgattccttc
    13681 taccttgatc gtcatattcc ttgctgcagc aaatcctgca taaataaccg attcatctga
    13741 agcgtataat ttttcccagt catacatgat gtcagaagcc atttcttctc ctgtttcagg
    13801 atcgtaataa tatgcctcta aaccagtatt cgtttttttc atctctacgt ccagtttagt
    13861 aatcgagttc gtggccggaa acagattccc atgaccgttt ccgaatatat tataagaacc
    13921 tgtttctttt tccaagaacc ctgctgaagt ctctaaaggt gttgtttcca ctgagacact
    13981 cccaggagct ggcgcagctt gcggatcttc agatgatatt cctgtgattg cccgtgtccc
    14041 caatccaagt cgcatcgtat atttgctgcc gctcgtgttg accacttctt gtctatccga
    14101 gtcccagaca tattcgatcc gactgcctag tatggcaaat gaatttgaat aaaatgcctg
    14161 cccaaacaaa tgattttttg gtactgcgtc tcttaccatt aaagcaaatc cttcttgggc
    14221 attcgtatag gtccaagatt ctatttcgat cgtcgcccgc atcctaaagt tgatgtcttt
    14281 ggggattgct tgataataat aggtcattcc atctgatccg cttgactgga attttccgcc
    14341 gttattcgtg gaagagagcc gcacgccgtc ttctaatggt tcaatggcat acctactagc
    14401 acttgtgctt tctccgaaaa caaccccttt ccactcttca tttgcttctt cagattgctt
    14461 ttttgtcttt tcattttctg attctttcat cataggatca tttccttctt ccgtatccat
    14521 atcttcacta gttcttggta tttcctccat tgttgacatc ccttcaatgt tcacgtgtgc
    14581 attttcttcg ttcacagact gttgttcgtt agcagcaact tcaacgacag aaattaaaac
    14641 cggggtactg agcataagga gattcatcat tagcgcactg tattttatac gtttttttaa
    14701 caagtgtatt cctcccgtaa caaattagaa agcgtttact taaagtatta cctaatttat
    14761 ttttactgtc aataaaaaac aaaattataa taagtgtatt ttataaaaag aaaaaatata
    14821 acttaagttg ttgataataa agagatttat taacggaaaa agaataagtt tttttaaaat
    14881 aacgtataaa aaagagcata aaacaaagtc gtttgtttta tgctcttctt ttattatttt
    14941 gtgcttttta ttttatcaaa tcactttgtt tttttataaa atttctgcgc ctagtgtgga
    15001 ctcaaaatgt ctcaatgccc attcatgtcc aacgggatcg aaacttgcca cggaatcttt
    15061 gaaaatatgg agtttgtatc ctagattata cgcatcgact gctgtatgca acacacagat
    15121 atctgtacat acgccggtca aataaatatc tgtgatctgg cgttctcgca aacggatgtc
    15181 tagatctgtt ccactaaaag cggaataatg ccgtttgtct atccaataaa cattttcctc
    15241 ttgctcatag tcttggtata aactttttaa agagccgtac aattcccgtc cacccgttcc
    15301 tatcacatta tgcggaggaa ataagcggtt ctccggatga aaggaatctt ttggatcatg
    15361 cgcatcaatg gcaaaaacga caaaatcctt ttgatcaata aacttctttg tatgagcagt
    15421 cagctttttt tcaattactt gtcctgctgt cccagtagtc agctttccat cctctgcaac
    15481 aaaatcatat gtgtagtcaa tcgagatcaa tgcgttcatc ttagtcctcc ttatacgaga
    15541 tgtttgacaa attcgatcac catttctgct gatcgtttgc ctgcgtcttc gataaattcg
    15601 tcaaagcttt gtgtcgccga gtgatctgct gtgtcgctca ttgcacgtac gatcaaaaat
    15661 gggatattga actgacgagc tgtttgtcca acagcggctc cttccatttc gcaagctaat
    15721 gcttcaggga aattcgttaa gatttctttg attttatctg gcgagtcgac aaatgtatca
    15781 cctgtaacga tcaatccttc ttttgcgttc aaattcgttg cttttgctgc tttaaccatt
    15841 tcactgcgca aatatgtgct tgcttcataa tatagaggca ttcctggtaa ttgtcctggg
    15901 ttgtagccaa atcctgttgc atctgcatca aaataagcta ctttgtctga tataacgatg
    15961 tcacctactt gtaagccttc accgattccg ccagctgaac ctgtattgat caccatgttc
    16021 acgccgtatt gttggatcaa cagacttgtg gtaatagatg ctaagacttt tcctataccg
    16081 gaacgtacga cgatcacttc gtggttacct aatgaacctg aaataaataa agcgcctgcg
    16141 cgttcccatg ataatggttc gcttaggttc tctctcaaaa tttttacttc ttcttccatt
    16201 gcgccgataa taccgatttt catcttttta attccttctt tctatataaa tgcgattgct
    16261 aaaaaaacca cacataacat taggataaca ataataagag atttcatcaa aaaagagttc
    16321 catttggtcg tcttttcccg ttcgctgatc cttgttttcg tgattggttt attctttttg
    16381 ctttctttac gataaaagct ggcaatcttt ttttcttctt gctgataagc tgtttctgct
    16441 tttcgctgtt tttttaatga ctcgcttgct tgtgcttgtt gtcgcttgcg aagttcgctt
    16501 cgcgtaataa gcggtccttt ggccataagc cgatccctct actttccttt ggtcaatagt
    16561 tcccagtatt gaatcgcata aatcgtcttt gcatcacaaa tcaactgatc tttcatcgct
    16621 tgttgcgcct cttcaagagt tagatgatac aactccagta cttcgtcttc atcttgtgct
    16681 aaaggattct cgaccttctc taccccttgt gcatgataaa tatgaaggac ttcatttgca
    16741 aaacctggtg aaagatacat cgaagtaaga tggctcagcg acttcgcacg atagccagtt
    16801 tcttcttcta attctctagc tgctgtcatt tcgggatttt gtccttctcc tggatcgatt
    16861 tttccagctg gaatctccaa aatcactttt tctaaaggtt ttcgaaattg tttgacaaga
    16921 agcagacgat cttgttcgtc aaaagcaatg atcccgacac cgccaggatg aaataccagt
    16981 tctctcttcg aagttcctcc atctggcaaa cgaacttcgt ctacagcaac atcaataatt
    17041 tttccgctat agatttcttt gcgactgatt gttttttctt caaattgctc attgttcagc
    17101 atatcttctc ctgctttctt tgtaatcata actgcttttt tttcattttt caatttttct
    17161 caaaaactcc ttattagttt accatagata aaccttagat tttctctaaa tatagaaaat
    17221 ccttctttag tccgatactc tttctagtag tccgtatgct aaaataagta tgtaatcgaa
    17281 taacgaaaag aattcaatac tcaacgataa atgacatgga gggtttatta tgataaaacg
    17341 aaattggccg tggcttctag cattggtctt gctatttctc tttgtccgaa acaatagttt
    17401 ttcttttgtt ctaggacttt tattgatagg tgtagtttta gttttgtggg gactctttgg
    17461 ttcgcgcagg aaaaagggaa aacaagaaga aattcctgca ctgactgacg aacttgaaac
    17521 tcactatgaa aaaagcggta tgacagcgaa tgaaatttca ttttttcgac aaacaatgaa
    17581 tcaaacaaaa aatgagatca atcaattgca aatgaatatg cagcagacag ccaaacttaa
    17641 agcaatcgat ctccgccatg atccagtcaa agcagcgaaa gctttattca aagaattggt
    17701 gaaagaacca aaccgactgc atgaagcaag tcagtttctt tatacgcatc tgccgaatct
    17761 agttgatttg acaaataagt ataatgagat caatgaccat gagatcaaaa acaaacagac
    17821 ctatgaaaaa ctagaagaaa gcgcgcagat catcgaccag ctgtcagctt taatcgcaca
    17881 agattatcaa aaattcgttg ctgaggatct agatgatatc gacgtagaga tatctgttgc
    17941 gaaacagagc ttaaagcgtg ataataaata tgaaccagaa caaaaggatt aactcttttg
    18001 ttcataaaag aagactcgga acattcagat gatgtctggc tgtttcgagt actttttcta
    18061 aagtagagac attcatgtaa gaatcgagaa cagattcaac aaggaggaat tttttatgga
    18121 tcataaaccg actgaaacaa aagatgttac accagttaac gatacacttg aagatttatt
    18181 gaacaacccg ttttcaactc caacagatac gctgaccact tcacaacaag cggagattga
    18241 tcagctccaa gagcaacaga cagctgctcg tttaattgac aaactaccgg cggaacgcca
    18301 agaacaagct aaacagctag cagcaaaaat cgatgctaat gatgcacagt ctgtcatcag
    18361 ctacggatcg gctgcccaag caaaattaag tgaattctca caatcgatgt tgaatcatgt
    18421 gcaagcacaa gatattggtc ctgtaggaga ttccttaact gaactgatgt atcgtctcca
    18481 agaagccaat cctgacgaac tgcgtgccgg agaagggaac atcttccagc gtgtcttcgg
    18541 gaaggtcaaa caatcgatct atgaagtgac agcaaaatat caaaagattg gtgcacaaat
    18601 tgacaagatt tcagtgaaat tagataaaga aaaagacggt ctgctaaaag ataatctgat
    18661 gttagaacag ctttaccaga aaaacaaaga ctactttgat gcactgaata tttatatcgc
    18721 tgctggagaa ttgaaaatgg aagaacttca aacaacgatc attccagaag cgatgaaaag
    18781 agcagaagaa actggcgatc agatggatgt tcagattgcc aatgattaca cgcaattcct
    18841 agatcgtctg gataaacgta cgcatgatct tcgcttagct agacagatca cgatccagca
    18901 agcaccacaa atccgtttga tccaaaacac aaatcaggcc ttggctgaaa agatccaagc
    18961 atctatcgct acagccattc cgctgtggaa aaaccaagtc gtgatcgcat tgaccttgct
    19021 tcgtcaaaaa gatgcagtaa ctgctcaacg ccaagtctct gaaacaacaa acgacttatt
    19081 gaagaaaaat tcagaaatgc tgaaaatctc agccatcgaa acagcgaaag aaaatgaacg
    19141 tggaatcgtg gatatcgaga cccttcaaac cactcaaaat gatttgatcg aaacaatcca
    19201 agagacattg cgtatccaaa aagaaggaaa agaaaaacgt cgtcatgcag aagtagagct
    19261 tggacacatg gaagaagatc tgaaacaaaa actgttggat ttaacccaat agatcacctt
    19321 tcgcttgtta ctaaaacaaa cgcccaacaa cagtttatta caactgttga tgggcgtttt
    19381 tcttatcttt cttctaactt ctccattgga gtctatttct cattcatctt gctgtgtcca
    19441 tagccatagc caaaataaat gatcagtcca ataaccatta cgataccgaa cacgatccat
    19501 gtcacagttt gcagacgaag catcaataca atacaagtca agatggagac gattggcaat
    19561 agcggaacga atggcacttt gaattctcca gcttttggtt tgccaaacat ttttctcaac
    19621 ttcagtaagc ctagactaac aagcatcaaa taggcgagcg ccatgatatt cgttagttcc
    19681 gctaatacat tcaatgggaa aaatccggca aagaatacag ctgccacacc agcggcataa
    19741 gtcgcttttt tcggtgtacg atgcttttga tcgatttctg tcataaactt aggaagtaag
    19801 ccatccttgc tgacagcaaa cagtaaacga gcaagtgaat acatcatcga gatcgttacc
    19861 gtcagtagcg ttaaaattgc ccctacagcg atcaccgcgc ccactgttcc atgtcctaca
    19921 tacctcattg caaaagccac tgggtcattg actcctagtt cagtatacgg aacgattcct
    19981 gtcaagatca gcgtcacgat gatgtaaagg acagtagcaa tcagtatcga accaatgatt
    20041 cctcttggca catctttttg cgggtttttg acttcctcgg ctgccattgc aacggcatca
    20101 aatcccaaga aagcaaaaaa cacaagagct gcaccatcta gcacaccgga aaaaccaaaa
    20161 ggcatgaatg gctgccagtt atcaggttta acgaaaaata ttcctacaat gataaacaac
    20221 gcgatgatac caaatttgac atataccatg gcattattca aacgtaatgc tttcttggct
    20281 tcaagcgaca cgatgtaagt gacgaatatc actacgagca ccgcaatcaa gtcaatgtat
    20341 gtaccatttt ccggattgta cccgccagtt aatgcttgtg gaagttcggc ccctaaactt
    20401 ttcaaaaaac cttgcatata gccagaccat ccggaagcaa cagaggaaac cgctagtaaa
    20461 aattccccga tcaacaacca ccctgtcagc caggcagcaa attcaccaaa tacaacatac
    20521 agataggcat atggcccacc aatcactggc actcgcgaag caaattctgc gaaacttagt
    20581 ccagacaaaa tgacaacgat tgcagctaag acaaatgaaa gtgacagtgc tggaccagca
    20641 ttttgattgg ccgcaacccc tgtcaccaca aatattcccg taccgatgat cgcaccaatt
    20701 ccaagcatga tcaaatcaga cgtttttagt tcttttttca tctcactcgt atgattgaga
    20761 ttgatctctt tcttacgaaa caaatccatt gaagttcctc cccattttag gttggaaaac
    20821 agcgattaaa ctgttttcat ataccctaaa aggataccac ctaaaatgac taaaaggcaa
    20881 ccaacaataa cataaatgag ctcttttttc gtttttcttt cgccaaggat gaaaatccca
    20941 cctaaagtag agataatgat ccccatttgt gaaagtgaga agccaacagc taaaccaagt
    21001 gatttgactg ttagaagcat gcagatgttt cctaagcccc ataataatcc acagatcatg
    21061 tttttccaca caaactgatc gactttaact ttacggaaag caaagaagct tgcgcctaag
    21121 atcattccga tactttgagg taagataatt gacatcgcat ccaaattcgc ccagttgacg
    21181 atgatggtat atacaccata accaatcgta gagaagatca atgctctaaa acccttacca
    21241 aaatcaagca ttttattatc tgtatctatt tttccttcgt catcttgtcg ggcagtcaaa
    21301 tacgcaccgc ttacaagaag agctaaagca ataataccgt atacataatc tttcgtttgt
    21361 gtccattcat ggaagaaaac ggctcccgca atcgtattca aaattaattg aaaaccagta
    21421 gacaatggta gccctacaga aacacccatg tacttcattc catggaactg tccattttgt
    21481 ccaacactcc aagctaagcc ggataaaaat ccgatcaaga aaatccaagg ggtgattaca
    21541 ggactaacaa cgaaaaagac aactaaagaa aacagaaatg caccgatcgt cattcctagt
    21601 gtttgttgat tggcatcgcc accaatttta ccacttacta aaccaatact accccatgcc
    21661 aacataggga ctaatgctat taaaatttcc ataaccaatc tccttttatt tcacatgata
    21721 agaaacttat cagcgaaaac gttaacaaca caagcacttt ttttcgtttc taacgatttt
    21781 ctaacgtttt tcattataaa aatttaagat tgggaaaaat aaagtcattg ttgtcccttt
    21841 tccttcagtt gattcagccg tcaaatggat acctaatttt tcggccagat tttttgctaa
    21901 ataaagtcct aatcctgttg aatgtgtttt gctaagtcga ccatttctcc cagtaaatcc
    21961 cttatcaaag atccgtttca agtcttccgc tgggataccg atccctgaat ctttaactga
    22021 aaggctgact ccttgcgctt ccttttctat gctcacaatg atgtttccgt gatccggcgt
    22081 atatttgatg gcattactaa gcaattggtt gaagataaaa gcgatccatt tagcatcggt
    22141 caaaacagta tgatcctcac caacgatctc gtagcgaata tttttttgga taaaataatt
    22201 tgcctgactg cgtatcacag gctgaatgat tccttttagt ccatattctt ggatgaggta
    22261 gtcccgcgag aaactgtcga gtcttgcata atacaaaact tgttctacgt attcatcgat
    22321 ttttgataat tcattttcta gtaatgtata cttttgatcg tcaatatcga actcgatcga
    22381 acgaactagc aatttagaag cggaaagtgg cactttgatt tcatggaccc atgagtcaat
    22441 ataatctttt tggtcttgtt gattactgat tgctgattgc attacttctt ggtgttcacg
    22501 gatcagctga ttgatatatt cttctaccag tttttcttct tctgtcctcc caccactcaa
    22561 ataattttgc aaaggggaag ttttttgtat gtcggctaat tttccccacc atcttctctt
    22621 gctcaagtaa ccacccagca gataaaaaat cagaaagacg ccctcaatca aagctaaata
    22681 agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc cacatcacaa agatcgtcaa
    22741 tgcaataaaa aacagccatc ccaagatcaa tgtccactga tctttcaaat atttaaaaaa
    22801 attcatctta accgctcatc ctttcaggga acgatatatc cctgtcctac ttttgtttcg
    22861 atatatcctt ggatacctgc ctgttcgatt tttcggcgta accgattgat gttgactgtc
    22921 aatgtattgt catctacaaa tcgttcgtca tcccacagtg ctcgcagcaa tttttcccga
    22981 cttaagatct ttccattgtg cttcatcaaa ataaataata gacgatattc atttttgctc
    23041 aaatcaatga tctctccgtt gatttccata cttccgttgt caacattcaa cgtaatgcca
    23101 ttgtggctca tcatttcact agataattct gtgtagttgt aagaacggcg aagtaatgca
    23161 ttgatttttg caatcagtac atcgatttgg aaatgttttg tcacaaaatc atctgccccc
    23221 atgttcatag ccatgatcat atccatattc gtattcctgc tggagataaa aatgatcggt
    23281 actttcgata cttcacgtat tttttgacac caataatagc catccaaaac gggaagatta
    23341 atatctagta atacaagctg tggttcttct ctttcaaagt ctgagaacac ttgattgaaa
    23401 tcggtcgttc caaaggtttc aaattgccat ttctcaagtt cctccatgat caattgtcga
    23461 atgatctctt cgtcttcgac taccatgatt tttgccataa aaaatccctc catctttctt
    23521 cttgctacta caatatcaaa gaagcccttg tgaaacaagt tggcttacct ggaaaatgtc
    23581 attttagcaa acgttaaaaa aaactggaaa tatgcgattt aatcgcgttt gtctcttcca
    23641 agcagcttaa atttattatg ttattcttaa taattggtct attatctgtt atttttcttt
    23701 cgttgttttt gtcttttaaa ttttcgataa caccaataac cgaaaagcgt tagcactagc
    23761 aatataccta gaaggagtat aagtaatcga taaagatggt atttttttgt ctgttctatc
    23821 tttgaagaga cttcttctgt aaagggaact cgtcttcctg tcactagtaa tcggtgagtg
    23881 ttcacggtgt aaggtgtaca agtgataagt gtgactagat cctgcttttc tctaattgct
    23941 aatgtatttg tttttgttgg aaggacaaca attttctcaa cgacttcata agcgagcatc
    24001 cgaccataca cttcaagata gaatcggtca tcttgctcca tttcttcaag atccgtaaag
    24061 agttttttct cagcaatacc gctatgggcg gtaatcacgg aatgtgtact ctctcctcca
    24121 gttggaaaag aggtgccttg caggactgtc gctcctctat ctaacaaaag atcattcgtt
    24181 tcatcaaata aaggcaagct aacattgatt ttcggaatat agatggctcc gatcatatgt
    24241 tgttcgtaat attcttttcc tggattttgg acatttctaa ccgcttgctc aaaaggatct
    24301 ttgacttgtc ccattcctgg gattactgat gtatgagcaa gcgtctgatt tttcttttca
    24361 tgcactgcta attgttgctc atgtttttct tctttatcgc gttcaagctg cttttgataa
    24421 ttctcgatcc ttctttgatc caaataattg ctgagagcat ctgctaaaaa tggatatgta
    24481 aaaactgcag ctccggcaag aaacatcact gccataaata attgtaataa aagactgatt
    24541 tgggtttgag gtctattacc tattttcttt ttcattttct tcaccttctt atttaaaaaa
    24601 actgcagcaa cctttataaa accgattgct gcagcttatt tttgttgctt atacttcaga
    24661 cttcatttta gatttacggt accagctgta cgctccaatc atcaaactta ttccgattag
    24721 taagaatgcc aagagcccgt tcccaccagt agcaggaagt gacccgcgtt tcatattcgg
    24781 tactctttct ggccctggat tttctactcc tacgatctga atttcttcat tatagcttcc
    24841 ataagagatg ataaattcag taaaggctgc ctcacctgtt ggaagtacat aaccttctgg
    24901 cgccatggtc tctttcattt gataggttcc ataatctaac ccttgaacct caaagtatcc
    24961 atttccatct gcgacaaatt ctgttgcttg tccgcttgtt gcccactcaa ttctatctgc
    25021 tcctgattca taaacagcta aaggcgactc ccctttataa aatattgcgt attcagaacg
    25081 gtcttcattc cacaattcga atcttgcatc tttaagcaat tcactgcttg atgcatcata
    25141 tttttcaaac tttcttccac ctgttgtgac tttctctggc ggctctactc cttcttcata
    25201 atcaaattca tcatttcgtc cgatttcaat aaccgctgtg ttgttatgga aatcatccgc
    25261 ttctagttc
//
LOCUS       DAYLZO010000029        24313 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.029, whole genome shotgun sequence.
ACCESSION   DAYLZO010000029 DAYLZO010000000
VERSION     DAYLZO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.029"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(557..1138)
                     /locus_tag="ACGVJG_001066"
     CDS             complement(557..1138)
                     /locus_tag="ACGVJG_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6557039.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(1321..1947)
                     /locus_tag="ACGVJG_001067"
     CDS             complement(1321..1947)
                     /locus_tag="ACGVJG_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFJ6557040.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1969..3300)
                     /locus_tag="ACGVJG_001068"
     CDS             complement(1969..3300)
                     /locus_tag="ACGVJG_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFJ6557041.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3455..3856)
                     /locus_tag="ACGVJG_001069"
     CDS             complement(3455..3856)
                     /locus_tag="ACGVJG_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFJ6557042.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3942..4148)
                     /locus_tag="ACGVJG_001070"
     CDS             complement(3942..4148)
                     /locus_tag="ACGVJG_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557043.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(4243..5745)
                     /locus_tag="ACGVJG_001071"
     CDS             complement(4243..5745)
                     /locus_tag="ACGVJG_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFJ6557044.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5763..6218)
                     /gene="argR"
                     /locus_tag="ACGVJG_001072"
     CDS             complement(5763..6218)
                     /gene="argR"
                     /locus_tag="ACGVJG_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFJ6557045.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(6316..6933)
                     /locus_tag="ACGVJG_001073"
     CDS             complement(6316..6933)
                     /locus_tag="ACGVJG_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFJ6557046.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            7154..8527
                     /locus_tag="ACGVJG_001074"
     CDS             7154..8527
                     /locus_tag="ACGVJG_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFJ6557047.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8602..9714
                     /locus_tag="ACGVJG_001075"
     CDS             8602..9714
                     /locus_tag="ACGVJG_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFJ6557048.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9786..11423)
                     /locus_tag="ACGVJG_001076"
     CDS             complement(9786..11423)
                     /locus_tag="ACGVJG_001076"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFJ6557049.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11591..12199)
                     /locus_tag="ACGVJG_001077"
     CDS             complement(11591..12199)
                     /locus_tag="ACGVJG_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFJ6557050.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12583..13734)
                     /gene="queG"
                     /locus_tag="ACGVJG_001078"
     CDS             complement(12583..13734)
                     /gene="queG"
                     /locus_tag="ACGVJG_001078"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFJ6557051.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13753..14505)
                     /locus_tag="ACGVJG_001079"
     CDS             complement(13753..14505)
                     /locus_tag="ACGVJG_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557052.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14646..15596)
                     /locus_tag="ACGVJG_001080"
     CDS             complement(14646..15596)
                     /locus_tag="ACGVJG_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFJ6557053.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15597..16436)
                     /locus_tag="ACGVJG_001081"
     CDS             complement(15597..16436)
                     /locus_tag="ACGVJG_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFJ6557054.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(16433..17179)
                     /locus_tag="ACGVJG_001082"
     CDS             complement(16433..17179)
                     /locus_tag="ACGVJG_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557055.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            17417..18787
                     /locus_tag="ACGVJG_001083"
     CDS             17417..18787
                     /locus_tag="ACGVJG_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFJ6557056.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18842..19738)
                     /locus_tag="ACGVJG_001084"
     CDS             complement(18842..19738)
                     /locus_tag="ACGVJG_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFJ6557057.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19852..20022)
                     /locus_tag="ACGVJG_001085"
     CDS             complement(19852..20022)
                     /locus_tag="ACGVJG_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557058.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(20019..20696)
                     /locus_tag="ACGVJG_001086"
     CDS             complement(20019..20696)
                     /locus_tag="ACGVJG_001086"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFJ6557059.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20787..21692)
                     /locus_tag="ACGVJG_001087"
     CDS             complement(20787..21692)
                     /locus_tag="ACGVJG_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFJ6557060.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21867..23321
                     /locus_tag="ACGVJG_001088"
     CDS             21867..23321
                     /locus_tag="ACGVJG_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFJ6557061.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            23337..>24313
                     /locus_tag="ACGVJG_001089"
     CDS             23337..>24313
                     /locus_tag="ACGVJG_001089"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFJ6557062.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKA"
BASE COUNT         6571 a         5049 c         4380 g         8313 t
ORIGIN      
        1 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
       61 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
      121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
      181 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
      241 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
      301 acctctccac taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
      361 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
      421 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
      481 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
      541 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
      601 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
      661 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt
      721 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
      781 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
      841 aaccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
      901 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
      961 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
     1021 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
     1081 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
     1141 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
     1201 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
     1261 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
     1321 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
     1381 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
     1441 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
     1501 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
     1561 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
     1621 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
     1681 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
     1741 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
     1801 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
     1861 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
     1921 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
     1981 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
     2041 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
     2101 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
     2161 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
     2221 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
     2281 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
     2341 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
     2401 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
     2461 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
     2521 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
     2581 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
     2641 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
     2701 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
     2761 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
     2821 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
     2881 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
     2941 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
     3001 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
     3061 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
     3121 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
     3181 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
     3241 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
     3301 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
     3361 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
     3421 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
     3481 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
     3541 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
     3601 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
     3661 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
     3721 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
     3781 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
     3841 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
     3901 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
     3961 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
     4021 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
     4081 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
     4141 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
     4201 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
     4261 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
     4321 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
     4381 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
     4441 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
     4501 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
     4561 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
     4621 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
     4681 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
     4741 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
     4801 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
     4861 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
     4921 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
     4981 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
     5041 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
     5101 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
     5161 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
     5221 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
     5281 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
     5341 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
     5401 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
     5461 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
     5521 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
     5581 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
     5641 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
     5701 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
     5761 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
     5821 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
     5881 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
     5941 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
     6001 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
     6061 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
     6121 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
     6181 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
     6241 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
     6301 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
     6361 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
     6421 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
     6481 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
     6541 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
     6601 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
     6661 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
     6721 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
     6781 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
     6841 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
     6901 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
     6961 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
     7021 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
     7081 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
     7141 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
     7201 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
     7261 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
     7321 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
     7381 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
     7441 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
     7501 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
     7561 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
     7621 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgtt
     7681 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
     7741 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
     7801 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
     7861 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
     7921 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
     7981 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
     8041 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
     8101 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
     8161 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
     8221 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
     8281 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
     8341 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
     8401 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
     8461 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
     8521 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
     8581 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
     8641 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
     8701 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
     8761 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
     8821 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
     8881 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
     8941 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
     9001 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
     9061 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
     9121 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
     9181 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
     9241 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
     9301 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
     9361 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
     9421 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
     9481 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
     9541 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
     9601 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
     9661 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
     9721 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
     9781 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
     9841 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
     9901 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
     9961 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    10021 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    10081 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    10141 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    10201 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    10261 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    10321 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    10381 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    10441 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    10501 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    10561 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    10621 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    10681 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    10741 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    10801 agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    10861 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    10921 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    10981 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    11041 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    11101 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    11161 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    11221 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    11281 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    11341 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    11401 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    11461 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    11521 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    11581 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    11641 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    11701 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    11761 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    11821 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    11881 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    11941 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    12001 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    12061 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    12121 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    12181 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc
    12241 tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa
    12301 aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat
    12361 aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa
    12421 aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg
    12481 atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca
    12541 acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg
    12601 caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa
    12661 tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc
    12721 ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg
    12781 caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt
    12841 cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat
    12901 cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca tctcttcatg
    12961 aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata
    13021 aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg
    13081 ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca
    13141 agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa
    13201 tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa
    13261 caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag
    13321 ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc
    13381 tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc
    13441 gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg
    13501 gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat
    13561 ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc
    13621 tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat
    13681 tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt
    13741 cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac
    13801 aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa
    13861 tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt
    13921 caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa
    13981 ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt
    14041 gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat aaaacatatt
    14101 cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat
    14161 tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc
    14221 gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc
    14281 gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag
    14341 ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc
    14401 ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa
    14461 gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt
    14521 ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca
    14581 taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc
    14641 acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc
    14701 atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt
    14761 ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca
    14821 ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc
    14881 atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa
    14941 tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc
    15001 tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca
    15061 tatgcttctg catttttttt atagagtgtc gcattttctg catctttttc agagaatata
    15121 cgtgcgattt cctccacata ttttattccg ttagataggt ctagccaagc atggggatct
    15181 gcttttgtat gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca
    15241 aaataatctt ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta
    15301 cctgtttcaa gattcaaacc gttgtataaa ataacatctg catcgcttgc ctttttgatg
    15361 tcttctggta gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata
    15421 tctatttgat ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg
    15481 actgacaact tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg
    15541 gctaatacca agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat
    15601 ctgcttcctt tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa
    15661 aatgcggtac atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa
    15721 aacacaccga caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt
    15781 aaatggttcg ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag
    15841 attgttccta ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga
    15901 taatggatca gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg
    15961 atcttcaatt ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct
    16021 gtcagcaaca aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa
    16081 tctgtcgcac tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag
    16141 acaattccaa tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact
    16201 gctgctgtta gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa
    16261 gaaatggcta cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct
    16321 cgtaaaataa taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc
    16381 aaagcgtatt gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt
    16441 tctctcctcc ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg
    16501 atgtaaagac ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct
    16561 catcgaaata ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg
    16621 aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta
    16681 cgaatggttc atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa
    16741 ctctctgaag ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc
    16801 ctactttttt caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa
    16861 ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa
    16921 gatccaattc acttcgctgt tccacgtagg ctattttttt acggactgag tttaccgact
    16981 gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa
    17041 atgttgattt tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg
    17101 tcaagttgat agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta
    17161 caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa
    17221 atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga
    17281 attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta
    17341 acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa
    17401 cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat
    17461 ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac
    17521 catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta
    17581 tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc
    17641 aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat
    17701 ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta
    17761 ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct
    17821 ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa
    17881 agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg
    17941 taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg
    18001 aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga
    18061 actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt
    18121 ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag
    18181 tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa
    18241 agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag cggcattatc
    18301 aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg
    18361 aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt
    18421 agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat
    18481 caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt
    18541 tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat
    18601 gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt
    18661 tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat
    18721 tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg
    18781 aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt
    18841 atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat
    18901 tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt
    18961 ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag
    19021 aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg
    19081 ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg
    19141 cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata
    19201 attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg
    19261 cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag
    19321 caactgctga taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt
    19381 caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa
    19441 gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag
    19501 ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg
    19561 tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta
    19621 tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt
    19681 tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt
    19741 ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag
    19801 caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt
    19861 gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat
    19921 ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc
    19981 gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt
    20041 tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg
    20101 atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga
    20161 ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga
    20221 taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg
    20281 aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat
    20341 tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt
    20401 gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta
    20461 tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg
    20521 atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga
    20581 taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat
    20641 tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct
    20701 tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc
    20761 gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc
    20821 aactgttttt tcaaggtatc caaagcaata ttcttaaaat tttcgttttt catacgttta
    20881 cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga
    20941 ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga
    21001 aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt acccatatga
    21061 tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca
    21121 acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag
    21181 gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa
    21241 aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc
    21301 atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg
    21361 tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc
    21421 cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga
    21481 atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct
    21541 ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga
    21601 aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga
    21661 tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata
    21721 atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt
    21781 taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata
    21841 tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg
    21901 ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat tttatggtat
    21961 tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta
    22021 cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg
    22081 gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg
    22141 gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta
    22201 ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt
    22261 tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa
    22321 aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta
    22381 ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata
    22441 ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta
    22501 ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg
    22561 aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc
    22621 ttgcccttgc ttatatcgct tatgcgattg ccaatgcagc aacaactgcc gtattattct
    22681 acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg
    22741 ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc
    22801 gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg
    22861 caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat
    22921 tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa
    22981 atggtatccg taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg
    23041 gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg
    23101 ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg
    23161 ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag
    23221 agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca gctgatacaa
    23281 tggaagatga acttcacgat gcagtagatc cttcccatta agaaaggatc ttgaaaatga
    23341 agattacaga aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca
    23401 agctagcact ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc
    23461 gagaattagt tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg
    23521 gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa
    23581 aaacttacca attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg
    23641 gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca
    23701 tttttactac tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa
    23761 tccgctatac tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc
    23821 aagatacttt attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc
    23881 agtcaatcga tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag
    23941 atagtattcc tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac
    24001 ctaaaaaatt agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa
    24061 tcgatcatcc atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta
    24121 gccccgataa aagaatccaa gtagatattg ctacagatgc ttctagtgtg gttattttca
    24181 ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg
    24241 gcatcacatt tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga
    24301 ttcatttaaa agc
//
LOCUS       DAYLZO010000030        24057 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.030, whole genome shotgun sequence.
ACCESSION   DAYLZO010000030 DAYLZO010000000
VERSION     DAYLZO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.030"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..2431
                     /gene="ileS"
                     /locus_tag="ACGVJG_001090"
     CDS             <1..2431
                     /gene="ileS"
                     /locus_tag="ACGVJG_001090"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFJ6557063.1"
                     /translation="CREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYEAAQVRVF
                     GKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGKDILDEDT
                     AFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIGWENVTVL
                     KEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYIAGSKYKL
                     SVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSYPHDWRTK
                     KPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWVISRQRAW
                     GVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTHPGSPNGE
                     FTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSITTSVAING
                     VAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVDYEADVRV
                     SMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVRLNQVIKE
                     IREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCMQTVFYQT
                     AVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTWTAFMDFR
                     DNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSPDYFEVMA
                     ADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCAKIVEDFY
                     PEAVAEGFEEK"
     gene            complement(2480..4006)
                     /gene="zwf"
                     /locus_tag="ACGVJG_001091"
     CDS             complement(2480..4006)
                     /gene="zwf"
                     /locus_tag="ACGVJG_001091"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFJ6557064.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(4021..4668)
                     /locus_tag="ACGVJG_001092"
     CDS             complement(4021..4668)
                     /locus_tag="ACGVJG_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFJ6557065.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(4852..5181)
                     /locus_tag="ACGVJG_001093"
     CDS             complement(4852..5181)
                     /locus_tag="ACGVJG_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFJ6557066.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            5358..6086
                     /gene="sfsA"
                     /locus_tag="ACGVJG_001094"
     CDS             5358..6086
                     /gene="sfsA"
                     /locus_tag="ACGVJG_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFJ6557067.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            6102..7115
                     /locus_tag="ACGVJG_001095"
     CDS             6102..7115
                     /locus_tag="ACGVJG_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFJ6557068.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            7115..8392
                     /locus_tag="ACGVJG_001096"
     CDS             7115..8392
                     /locus_tag="ACGVJG_001096"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFJ6557069.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            8455..11157
                     /locus_tag="ACGVJG_001097"
     CDS             8455..11157
                     /locus_tag="ACGVJG_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFJ6557070.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            11309..11626
                     /locus_tag="ACGVJG_001098"
     CDS             11309..11626
                     /locus_tag="ACGVJG_001098"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFJ6557071.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            11656..11976
                     /locus_tag="ACGVJG_001099"
     CDS             11656..11976
                     /locus_tag="ACGVJG_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFJ6557072.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            12084..13544
                     /locus_tag="ACGVJG_001100"
     CDS             12084..13544
                     /locus_tag="ACGVJG_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFJ6557073.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            13612..13833
                     /locus_tag="ACGVJG_001101"
     CDS             13612..13833
                     /locus_tag="ACGVJG_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557074.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            13864..14046
                     /locus_tag="ACGVJG_001102"
     CDS             13864..14046
                     /locus_tag="ACGVJG_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFJ6557075.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            14046..14459
                     /locus_tag="ACGVJG_001103"
     CDS             14046..14459
                     /locus_tag="ACGVJG_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFJ6557076.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            14582..15763
                     /locus_tag="ACGVJG_001104"
     CDS             14582..15763
                     /locus_tag="ACGVJG_001104"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6557077.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(15834..15998)
                     /locus_tag="ACGVJG_001105"
     CDS             complement(15834..15998)
                     /locus_tag="ACGVJG_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557078.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            16152..16225
                     /locus_tag="ACGVJG_001106"
     tRNA            16152..16225
                     /locus_tag="ACGVJG_001106"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16186..16188,aa:Arg,seq:cct)
     gene            complement(16294..17433)
                     /locus_tag="ACGVJG_001107"
     CDS             complement(16294..17433)
                     /locus_tag="ACGVJG_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6557079.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(17547..18131)
                     /locus_tag="ACGVJG_001108"
     CDS             complement(17547..18131)
                     /locus_tag="ACGVJG_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFJ6557080.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(18214..18636)
                     /locus_tag="ACGVJG_001109"
     CDS             complement(18214..18636)
                     /locus_tag="ACGVJG_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFJ6557081.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADKIAIMILLNIFIENELTDE
                     SQFKLENFMEFYAINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(18654..18974)
                     /locus_tag="ACGVJG_001110"
     CDS             complement(18654..18974)
                     /locus_tag="ACGVJG_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6557082.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            19274..19414
                     /locus_tag="ACGVJG_001111"
     CDS             19274..19414
                     /locus_tag="ACGVJG_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557083.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            19712..19969
                     /locus_tag="ACGVJG_001112"
     CDS             19712..19969
                     /locus_tag="ACGVJG_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFJ6557084.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(19940..20248)
                     /locus_tag="ACGVJG_001113"
     CDS             complement(19940..20248)
                     /locus_tag="ACGVJG_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557085.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            20326..21072
                     /locus_tag="ACGVJG_001114"
     CDS             20326..21072
                     /locus_tag="ACGVJG_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFJ6557086.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            21087..21290
                     /locus_tag="ACGVJG_001115"
     CDS             21087..21290
                     /locus_tag="ACGVJG_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557087.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            21306..21644
                     /locus_tag="ACGVJG_001116"
     CDS             21306..21644
                     /locus_tag="ACGVJG_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFJ6557088.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            21631..21801
                     /locus_tag="ACGVJG_001117"
     CDS             21631..21801
                     /locus_tag="ACGVJG_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557089.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYKTKKPVGRD"
     gene            complement(21899..22564)
                     /locus_tag="ACGVJG_001118"
     CDS             complement(21899..22564)
                     /locus_tag="ACGVJG_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557090.1"
                     /translation="MSLIDFIKQFDWNNIVKTIKDFILPITSLVFSTFAFYISYTDRK
                     NKKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSI
                     SGIPDHLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITI
                     PPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLD
                     EFD"
     gene            22619..23317
                     /locus_tag="ACGVJG_001119"
     CDS             22619..23317
                     /locus_tag="ACGVJG_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFJ6557091.1"
                     /translation="MQELVILKNKEAVTTSLQVADSFEKEHKNVLRDIEKLKEDVLNF
                     EQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNIKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            23495..23836
                     /locus_tag="ACGVJG_001120"
     CDS             23495..23836
                     /locus_tag="ACGVJG_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557092.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            23829..>24057
                     /locus_tag="ACGVJG_001121"
     CDS             23829..>24057
                     /locus_tag="ACGVJG_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFJ6557093.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFL"
BASE COUNT         7932 a         4080 c         4728 g         7317 t
ORIGIN      
        1 atgtcgtgaa tatgcacttt cccaagtaga taagcaacga gcagatttca aacgcctagg
       61 agttgcagga gattgggaac atccttatgt tacattagat ccttcttatg aagctgcaca
      121 agtacgcgta tttggtaaaa tggctgaaaa aggctatatt tacaaaggat tgaaaccaat
      181 ttactggtct ccgtcaagtg agtcttcttt agcggaagct gaaattgaat ataaagatgt
      241 aaaatctcca tctatttttg tagctttcaa tgtcaaagac ggcaaagata ttcttgatga
      301 agatacagct tttgtcattt ggacgacaac accatggaca ttgccagcaa accaaggtat
      361 tgctgtaaat ccaaaattta cgtatgtatt ggtggaagcc gatggccgta agtttgtcgt
      421 agcgaaagat ttgctagaaa cagtccaatc agaaatcggt tgggaaaatg taacagttct
      481 aaaagaattt gctggacaag aaatggaata tatgactgca agtcatccat tctatgaacg
      541 tgaatcactt gtgattttag gcgatcatgt tactttagac gctggtactg gattagtcca
      601 tactgctcca ggacatgggg aagatgacta tatcgctggt agcaaatata aactatctgt
      661 agtgtcacct gtagactcaa aaggattgtt tacagaagaa gcacctggat ttgaaggtat
      721 cttttatgac aaagcaaatc ctatgatcac tgatctatta aaagaaaaag gagctttgct
      781 gaagctagat ttctttactc atagttatcc tcacgattgg cggacgaaaa agcctgtgat
      841 ctatcgggca actccacaat ggtttgcatc tatcgataaa ttccgtcaga atattttgga
      901 tgaagtagaa aaagtagagt gggttatccc atggggtaaa acacgtttgt ataatatgat
      961 ccgtgaccgc ggcgattggg taatttcccg tcagcgtgca tggggagtac cacttccgat
     1021 tttctatgct gaaaatggag aagcaatcat cacaccagaa acaatcgaac atgtggcaaa
     1081 attatttgct gaacatggat caattatctg gtttgaaaaa gaagcgaaag aacttcttcc
     1141 tgaaggattt acccacccag gttctcctaa tggcgaattt acaaaagaaa aagatatcat
     1201 ggatgtctgg tttgattctg gttcttctca tgaagctgtt ttacgtcagc gaccagaatt
     1261 atcattccca gcagatatgt atttagaagg atcagaccaa tatcgtggct ggttcaactc
     1321 aagtatcacg actagtgtgg ctatcaatgg tgtagcacca tacaaatcag ttatttctca
     1381 aggatttgct ttagatggtg aaggccgtaa gatgagtaaa tctttaggaa acatcattgc
     1441 accagaaaaa gtgatcaaac aatttggtgc agatatcctt cgcttatggg tatctagtgt
     1501 agattatgaa gcagatgttc gtgtttctat ggatatctta agtcaagtgg cagaagtata
     1561 tcgtaaaatc agaaatacga tgcgtttctt attagcgaat acagaagatt tcgatcctaa
     1621 aaaagatact gtttcgtaca atgatttacg ttctgtagac aaatatatga ccgttcgatt
     1681 gaaccaagta atcaaagaaa tccgagaaga agggtacgac aaatataact tcatgcatat
     1741 ttaccgtaca gttatgaact tcttgacggt tgatctatcg tcattctacc tggattttgc
     1801 taaagatgta gtttatattg aagcagaaga tgattatcaa cgtcgttgca tgcaaactgt
     1861 tttctatcaa acagctgtag ctttgacaaa attattgact cctatcattc cgcatacagc
     1921 agaagaaatc tggtcattcc tgaaagaaga agaggagtac gttcagttaa gcgagtttcc
     1981 tggttatgaa gagtttgcaa accaagaaga attgatggat acctggactg ctttcatgga
     2041 cttccgtgat aatgtcttaa aagcattgga agaagcaaga aacagcaaat tgattggtaa
     2101 atctttggaa gcaaaactaa ctgtttatcc aaaacaacaa gttcgtgaaa tgttaaaagc
     2161 tttggatgca gatattgcac aattattgat cgtttcacca gactactttg aagttatggc
     2221 agcagatgaa caagtgccag ataacgctat ggtatttgat gatgttgcta ttacagtgga
     2281 aaaagcagac ggggaaactt gcgatcgctg tcgccaagtt cgtaaagatg taggagtaga
     2341 tgaaaaactt cctcatctat gcggtcgttg tgcaaaaatc gttgaagact tctatcctga
     2401 agctgtagct gaaggattcg aagaaaagta aaagggaaaa gggggtgtga catgttcttt
     2461 gtcacacccc cttttgttat caatcgttta attttccacg ttcacgatac cagttatctg
     2521 gttgccagat ccattcgaat ccgtcttttt caagtagttc gaaagctgct ttaggtccca
     2581 ttgatccagc tttgtagttg ggaaaatccg gttgtgtttg gtcccaagct tttcgaatga
     2641 catccacgat atgccaagat tgagcgactt cttcccaatg agagaagttc gttccatcac
     2701 cattcaacgc atctaatagt aatttttcgt aagcttctgg gctattttca accatttctg
     2761 cactatttct aaattccaat ttgattggtt ccgtttcaaa tccttgccct acttctttcc
     2821 cattcaagga taaagaaaat ccttcagttg gctgaatata gatcgttaga acattaggtg
     2881 gaagtgtcgt atcgtcacat ggttcatcga cagatgtttt gaatacatta acaggaactt
     2941 gtttgaatac gatgttgatc cgagtccctt tttcagtcaa acgtttgcct gtacgtacat
     3001 agaaaggaac acctgaccaa cggaagttat cgatagtgaa tttgcctgcg acgaatgttt
     3061 cagtctctga ttgagcatcc actttgtctt cttcgcgata gcccttgaat tgtacaccat
     3121 ccaattcacc ttgagcatat tgcccgcgaa cgaagttttc taacacttct tcttcgttat
     3181 aaatgcggat agctttcaat gctttgatct tttctgtacg gatttctttt tcggaaaatg
     3241 ctactggcgg ttccattgca agtaatgcta caacctgtaa aatatgattt tgcaccatgt
     3301 ctttaagtgc gccactatga tcgtagtagc ccccgcgatc ttctacaccg aggttttcgc
     3361 caaaagtaat ttggatattg tcgatatagc gattgttcca ttgagcctca aaaatattat
     3421 tggcaaaacg gatagctgag atgttttgga tcatttcttt tcctaaataa tgatcaatcc
     3481 tgaaaatatc ttgttctggg aatacttctc tgatttgctg gttcaattca aaagcagact
     3541 ggtaatcaga accgaacggt ttttctatga tcaaacgatt gaagccattt tttgtcataa
     3601 tattttgtga tttcaaatga gaaacgatcg ttccaaaaaa ttgaggtgcc atagccaaat
     3661 aatagacacg gttaccgttt aattgatagt gtccatctaa tcggtcagac aattctttta
     3721 atgtctgata atgcgttgaa tcatttacat catgggattg atagtaaaaa tggcttgcaa
     3781 aggtagttgc ttcttcatct gaaggattca atgcacggat tgtttctttg acaacttcac
     3841 ggtagtgctc atcgctccag ggacggcgag cggttccgat tacagcaaaa tgctcgccta
     3901 aatttccttt tctgtaaagg cggaacaaag acgggtacaa tttacgctgg gctaggtcac
     3961 ctgtaccgcc gaatatcgta aacaatacat ttttttcatt agacatgtgg atacactcct
     4021 ttatatttta tcgacaaaaa tcgtgctggc cgccttaaag ctgacgggga ttttctttct
     4081 atcattttgg atagtgatcg gtccttcata agctgcgatg tcaacgatct catattcttc
     4141 gttgatattc aggtcaatag tgactaagta atccaacaat tctttttcat caagcactcg
     4201 tgcgatacgc acttttgtac cgacaggata ggactccaat gtttgtcggc gttcttcatg
     4261 gacaacttcc tcttttttag gaatcgatcc tccatgagga caatgttcag gatgattcaa
     4321 atagttttct aaatgatctg caagatgttc cgaagtcaca tgttccaata cttcagcatc
     4381 atcatggact tcattccagc tatagtctaa atgttcaact aaaaaaactt cccataagcg
     4441 atgtttgcga atcagactgc tagctttttg cagtccgctt gcagtcaatt gtacaccttg
     4501 ataaggggaa tgttctacaa gctcttcttt cacaagttta gaaatcattt cactgacaga
     4561 tgcagctgag acatctagac tagatacgat ttgtttattg ttgactttcg ttgtatctcc
     4621 acctagttct aaaatgattt tcaaataatc ttctcgattt ggtgtcatat tcgatcatcc
     4681 tttcatggta aaaaaccatc attagtaagt gtaacaaaaa aaacgttaat tgactatttt
     4741 tctaaataaa aaacaaagga tttttagacg tttttacaac aaaaaacgaa agatgataga
     4801 gaagactcat tctttcgttt ataaaggata tatatataca ttgggaggga tctatttttc
     4861 agataacaac ttatatgaag tgtctgcaga ttcgtcataa agagtttgtt cgggataaga
     4921 aaagataaga taaaaaccat actgatcacg tacgactaaa cgatattcat ctatttctac
     4981 cacagatgtg ccaagagact gaccgttgat caaaaacgtg taatcagatg taatctgtaa
     5041 ttgttgtttt tgctggctat cactaaagaa ccatgtacct tgatactttg agagtccttc
     5101 tgacttttcc gcttgttttt tctttttcaa gtaaataaaa cttgagagaa cggtaatgag
     5161 tgaaccaaga aaatagatca ttttttttat caccaaatca ctcctttatg agaaaataat
     5221 cgcatagaca tattctttct tctttatttc attgtaccga ataaaagcta aaatttctct
     5281 aagactttcg actggttttt ttactgtttt tctacaaatt atgataggat acatagtagg
     5341 aagaaaggtg acataatttg aagtatcagc aagtggaaat agcttttttt attgaccgcc
     5401 cgaatcggtt tatagcacat tgtatgaaaa aagacggaga aataattatt gcgcatgtca
     5461 aaaatacagg tagaggaaaa gaggtcctga taccaggcgc tgaagtggct attgtatttg
     5521 ctcctggacc taaacggaaa accgcctatg atctgattgc tgtaaaaaag aagggtgact
     5581 ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat tgatggtatt ttagacggca
     5641 cgatcctttt accaggatta actggtccat ttacggtata caagcgtgaa gtaacttatc
     5701 ttcactccaa atttgatatt tatttagaga cggtggtcgg acaaaaagca tttgttgaag
     5761 tcaaaggtat gacacttgaa aataaagcga ttggtgcttt tcctgatgcg cccacactca
     5821 gaggattgaa acatatagga gaattaacgt atgccgcaca agatggttat gccgcttatg
     5881 tcttgtttat tgctcagttc gaacatcttc atctggcaac gatccatgaa gaaatgcaac
     5941 cagcactagc tgatatggtt cgacatgcgc agcaaagcgg agttcaaatc ctcgcctata
     6001 attgtcaagt atcggcagat gaggtaactg tcaagcaggc cattccattt gatgtagata
     6061 tcccttttga agatcctata caatagaaag aaggtaaaat aatgatcaac ttaggtatta
     6121 tcggaacaaa ttggattact catcaatttg tccaagcagc tttaagcaca aaaaaatatg
     6181 aattgacagc ggtatattct agacatttgg aaaaagcaca ggaatttggt tctcattatg
     6241 aaggggatat cgaattcgca acagatttga agactttttt caatattact catatggaca
     6301 ccatttatat tgcttctccg aatagtcttc actttagtca agctaagcaa gcaattttgg
     6361 caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac gccaaaagaa atggatgaaa
     6421 tcatccattt ggcaaatgaa cacaatgtgt atttttttga agcagcaaga aatatccatg
     6481 aatcaggttt taaaaagatt gccgattact taccattaaa agacgagata atcggtgcaa
     6541 atttcagcta tatgaaatat tcttcgcgct atgaccaagt attagatgga gaagaaccga
     6601 atatcttctc gactcatttc tcaggtggtg cattgatgga tttaggtgtt taccttgtat
     6661 atgcagctgt tgcttggttt ggaatgccaa aagaagtcca ttatttccca agaaagattt
     6721 ctactggtgt agatggtctt ggatggggga tcttacgtta tgagaatttt gatgtatcga
     6781 tccagccagg taaaatcggt gattcttatt taccgtcaga gatttatttt gactcaggaa
     6841 cgctagtgat gaatggagtc aatgcaattg aacaagcaca atttcatgat cgcgcgcata
     6901 aagaaatcat tgatttggcg gtaacagctg cagaaaatcc aatggcagat gaagcagagg
     6961 attttgccaa tgtcatggct catccgaaag atccagcttg gggattgttg tatgaagagt
     7021 ggatcgagct agctagaaat gtgaaccaag tgatttatga tttaagaaaa aatggcggca
     7081 tcaaattcga tgcagataat gaggaagatt tttaatgaac gaatttgtac aagtatttcc
     7141 cgaaaattgg cagaaaaaat atgaggataa agggtttatc tcaccttctc ttattcaacg
     7201 agcagttttt cagccactaa gtaataaaca aagtatagta gcggtttctc ctacaggttc
     7261 aggtaaaaca ctggcttatc tttggcctct actactaaac gtggaaccag gtgaagcaag
     7321 tgcgttggtc atttttgctt cttctcaaga actagctatt caagtagcag atgttgcacg
     7381 tgaatgggga aaagacaaag agttgaaagt ccaatctttg gtgggcggag ccaacgtcaa
     7441 acgtcagatt gagggtttga aggaaaaacc agaaatcttg attggtacac caggaagaat
     7501 attggaattg atgaaagcaa aaaaaataaa agcacatcaa gtgaaaacaa tggtatttga
     7561 tgaagcagac cagctatttg acgctgggaa cagccaaatc attgatcaaa ttcttcatca
     7621 agctccaaca gagtatcagc tagctttttt ttcagcgaca gcagatcgtt cccttgaaag
     7681 cattgaaaaa atcactggga aaacgtttga gacaattgac gtgacagcag aggacgattc
     7741 tcgaaaagga ctgagacatt actttttacg tgtaccgata cgaaagaaag atgactactt
     7801 gagacgtctc acgcatatag aagattttca tggtctgatt ttctttaatc aactgactga
     7861 attaggcgta atggaagaaa aattgctgta tagaggtgta ccggtaggaa gtctggcctc
     7921 tgatcagaac aagctactcc gaaaagcagc gctagaacaa ttcaaaaaga aaatggtcag
     7981 agcacttttg accacagatg tggcggctag aggtttagat attacaggtc ttccttatgt
     8041 agtgaatgca gaagttccgt taagtgaaga agcttatttg catcgttcgg gaagaactgg
     8101 tcgaatgggt aatgaaggaa tagtaattac actcgtacaa gataataatc taagagattt
     8161 aaaacggctt ttacgtccat caaatataac acttgaagag atttttttat acggaggtaa
     8221 gctgcaaact gagcagccaa ccaaagaaga tgcagctcct gcagtaaata aaaagtatgc
     8281 gaaaactttt tctggaaagg agcagaaccc agaatataac aaagaaaaaa taagaaaaaa
     8341 agttaaaaat aaatcaaaaa aagagagaaa taaaggagca aggcgcaaat aagcgccttt
     8401 ttatgctatg attatgaaaa cgggaacaaa attggttgcc agaggagggg ttatgtgaaa
     8461 gaacgtcaag aagagatctt gcagctattg acagaaaacc aacaaggatt gacagcaagt
     8521 gatgtagctg agcgcttaac tatcgatcga agcaatgcaa gccgatattt aagtgaacta
     8581 tacaaggccc atcatattgt aaaaaccgct ggccgaccag tcgtttactc tttgccaacg
     8641 gaaaagtcaa aatctgatga agtgcatgtc gactcttcta cgcaagtgac ttttgaaaca
     8701 ctcgttggag aaaatgattc tttaaaagtc agcatacagc aggcaaaagc tgcgattctt
     8761 tatccaccgc gggggttgca tacgattatt tttggagaaa ctggtacagg gaagtcgatg
     8821 tttgctgaat gtatgtatca ttttgcaatc gattcagaga tgctttctgc agatgcgcca
     8881 tttgtttctt tcaattgtgc ggattatgct caaaatccgc aactgctctt tggacacatc
     8941 tttgggatca aaaaaggggc ttacacagga gcagctcaag atagtcccgg tctaatagcc
     9001 aaagcagacg gcggtatatt gtttttagat gagatccacc gtttaccgcc ggaagggcag
     9061 gaaatgctgt ttactttcat tgataaaggt atttaccgtc cgcttggaga aagtgcacaa
     9121 gtacatgaag catctgttca gatcattggg gcgacgactg aatcttcaga aagtttctta
     9181 tctacgttca atcgacggat tcctatggca atcaccttgc cgaatcttgc ttcacgttca
     9241 ctggatgagc gttatgaaat catctctctt tttatcaaac aagaagcgaa tcgtttgaat
     9301 caacgtatcg atatcgaaaa agaagcgatt cttgccttca tgctttatga tgcggaagga
     9361 aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg caaaatcttt tttacattac
     9421 cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag aactttccct tcaagtacaa
     9481 aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg accgattcat ggacagcaaa
     9541 agtcagtatt tgacatttga accaggattt gctgatgttg tctggtcaca agatccagaa
     9601 cgaaacatgc aggtctataa cgatattgag gaaaaagtca cttctctttc cgaaaccggt
     9661 gtggagaaca ttgatctaga gaccctcatt tcaaaagatg tggatgctta tttccagaca
     9721 tatgtcaaag aattgactaa aagttcgatt cccaaagatc ttttgccaga tgaaatctgg
     9781 cggttaacca atcaattgta tgatgtggct gaaaaacggt tagaccgtat ttataatgaa
     9841 aaagctcgtt ttgcttttgc tttgcatctg caaagtacat tggatcgtgt gagagaaggt
     9901 catatgatcg tccacccaga tttgaataac gtacggaaaa attttaagag ggaattccag
     9961 gtagcaatcg atctttcaac gatcattgaa gaagaagaac aagttgagat tccttttgat
    10021 gaaatcggct ttattagtat gtttctttct atcaatctag gagaaagtga gcctgttaaa
    10081 gaaaacaaag tagatgttct catattgatg cacggcagac agactgcttc aagcatgttg
    10141 gagatggcac aagaactatt aggaacaaaa gtagggaaag cttttaatat gccactggtt
    10201 atggaagttc aaacaatgta tgaagaagtg ttacaatatg tcaaaaacta tcaagagcat
    10261 ttgacgaatg gcctactctt gttaacagat atgggatcat taaacacctt cagtgatttg
    10321 atttatgaag agactggcat tcggacgaaa gctatcacga tgacaagtac catgattgtg
    10381 atagaagcga tacggatggc aagtgttgga agaagcttag aagatattta tcaaaatatc
    10441 cagctttcct ttgaatcgat cgtacgtgat cagttcaaag cggaaaagtc tgaaacttta
    10501 cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg aaggagtagc tgccaaatta
    10561 tatcagcgta ttatgccagt gattaatcaa aataaggtag aactgatcca gatgcagttt
    10621 ttagaacgtg aatcattcaa aaaacacatc gatgccttat tggaagaata tgagatcaaa
    10681 gcaattgcgg gaacagttga tgttgattat caaaatatcc catttttttc agcctatgac
    10741 gtttttgatg atgaaaaact aaatatttta aagcgtatag ctgatgatga gatcccgaca
    10801 gctaaaattg tcgattcact tgctggatca ttaagcgatg tcttttctat tgatgaactg
    10861 atccgttcat tgcaaaagac agttcagcag atccagaccg atttgtcgat tatcgtggaa
    10921 cctggagtag atactggaat cgttatccat ttagccttca tgttagacgg attgatcaaa
    10981 caagaaagcc atcggcaatt tgagaaactg tctattttct caaaaattta tcgtttggaa
    11041 atggatatcg ttcgggcaaa cttgatggca ctagaacgaa aatatcaggt aaaaattcca
    11101 gaagatgata tcgcatttct tacacaaatg tttcttgaaa ataaaataaa aaaatgaaca
    11161 cactaatcat actgtttaca cactaaacat ctgtgtgcgc actataaaaa agagctaaaa
    11221 agcatgaaaa cgctatttca gctctttttt ttattggcat gcttcttgcg ttatataaaa
    11281 gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa acaatcatgt tagtttgttc
    11341 agcagggatg agtacgagtt tattggtcac aaaaatgcaa aaagcagcag aagaaaaagg
    11401 gatggaagcc gatatttttg cggtctctgc ctcagatgca gataataatt tagaatctaa
    11461 gaatgtcgat gtattattat taggtcctca agtacgtttc atgaaagctc aatttgaaca
    11521 aaaacttgcg cctaaaggta ttccattaga tgtcatcaat atgcaagact atggcatgat
    11581 gaacggagaa aaagttcttg cacaagcaga aaatctaatg aaataaatca agtatgcagt
    11641 tgggggtata acaacgtgga agatcaacaa aatctagaag ccatcatggg gttaatcatg
    11701 tttggcggta atgcaaagag cgatgcaatg gaagcgattg cggctgctaa aatcggtgat
    11761 tttgagttag cggataggaa aatcgctgat gcagaagaat cacttataca agcgcatcat
    11821 gcacaaacag acatgttgac acaagaagca caaggaaatc atatgcaagt gacattattg
    11881 actgttcaca gtcaagatca cttaatgaca tctattgcgt ttactgattt agcaaaagaa
    11941 atcatcgaac tttatcgtcg cgtatacagc gaataaatac aacttctttt ttgaacggaa
    12001 gaagaaaaat ttttgaacgg attttttatt ttgttataag gagaactctc tttatataaa
    12061 aaacttatag gaggaagtaa aaaatgaacg gactatcaag ttggatggag aaatacatcc
    12121 tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt tgctttgcgt gatgctttta
    12181 tcggcatact gcccgcaaca atggcgggat cagtcgctgt aatgatcaat gcaattattc
    12241 gtgatttacc gcaacagttt aacagtacct atgctgacag tttagcagca gacaaaggaa
    12301 tctttgccgt attgaatgtc atcatcggaa tcaatggttt tgtctggaac ggtacactag
    12361 caatcgccgg attgatcttt gccttttctt gggggtataa tcttgcccga gcttatggag
    12421 taaatgatct agctgggggg atcgttggtt tagctacctt gattcaaggt atttcctttg
    12481 gtccagcatt atcagcagca ttagatgtga atgttccttc aaatgtcgca gatgcaatca
    12541 atagttctga gattggtgca acgattgcag atggcacact gtcagttgga ttatggggtt
    12601 ggctaaaact agatcatttg aatggaaacg cttattttac agttatgatc atgggagcta
    12661 tttcagtcat tattttttgt aaattgatgc ttgccaacat tacgatcaaa atgcctgatt
    12721 cagtaccacc agcagtatct aaagcatttg cagccatttt accagcgacg atcgctttat
    12781 acgtcattgc tattatcaat tatatcgtag gtcgtgtagc tgatgggcaa ctgatcattg
    12841 atttggtgca aaaatatatt gcagaaccat tcctaggatt atctcaaggg ttgggagcag
    12901 tattgatcgt cacagtattt gttcaagtat tctggttctt tggtattcat ggatcaaatg
    12961 tattggcacc agtgctagag ggaatctggg gacaagcaca actaatcaat attgatattt
    13021 tccaaaaagg ttataacgga aaaactggta caccagccgt gttagatgca atcgaagatg
    13081 gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt tgcatggttt ggcggatcag
    13141 gcggaacgat cgtcttactg atagctatct tgattttctc taaacgtgca gattatctaa
    13201 cagtagctaa attatccatt gggcctggta tcttcaatat caacgaacca gtgatgtttg
    13261 gtctgccaat cgtattgaat gcaatcatgt ttatcccatt cttgattgct ccagtagtag
    13321 caacagcaat tggatggaca gctacttatc ttggcctagt cgctccggta tcgcagcaag
    13381 tcacatgggt agtaccgccg ttcttattgt cattcttagc aacaggagcg gattggcggg
    13441 caccgatcgt aacattagta tgtatggtcg tgactttctt gatttgggca ccatttgtca
    13501 tctctgcaaa caaaatggac cctgccgcag gcgaacaaca ataagatgaa cgtgaggagc
    13561 gggacgcttt aagtttcgtt cctcataatg aattttcgag gaggaactga aatgattaca
    13621 attgccagtg caatgcaaca agatgaaatc aaagaattgt tagaaggata caatgaagaa
    13681 ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc tgttctttga aacaagtgca
    13741 gaagatccgg aagttgcggc taaaaaagca aaggaactaa tcaaagcaca gccttgggga
    13801 acagtccttt attttcaatc aacagctgtt tagaaaatgg tttaaaaaga taggatggtt
    13861 gagatgataa aaaacggttt atttttatgt agtattggtt ctttgatcgt gttgtatagt
    13921 ttgaatccac attcgcaaag gtatgatttg tatagtatgg tcacaggtct ttttctaaca
    13981 ggattaggaa tctatttctt catgaagggg aaaaaaagag aagaagcaaa ggagaaaaat
    14041 aactaatgga aatcacgaaa aaattaaata ttccagcatc gtttttttac gataaagtta
    14101 tggactctgt ccttttcgat gtacgtaaag cgaccggcaa atcagtaact agaaaacagc
    14161 tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa ccgggctcgc ataaaaatcg
    14221 aagaagtcat tgaaaaccag tcttataagt ttcgtacatc gacaacgaag aatgaattcg
    14281 tcgttcatta tcaaatcaaa ccattggacg attcgacctg tgaggttcat tacacagaaa
    14341 aaatggagtc ttatggcatg cttcaaaagt tgaatgatat gcttttagga acaatcttga
    14401 tgtattttaa acggagacgt ttcaaaaaaa tgctagatat gatcgaagca acgtattaaa
    14461 tcaatgaaac atggtactat ttagttattg aaaaatgaag taagctagaa ggaatttttc
    14521 ataggaagat agatctttcc tgcgaatcct cactagcttt tttgtaggaa ggggcagcaa
    14581 aatgacaaac aaagtaagag agctaggtgt atcttcgatt gaggaaatgt ttgatttagc
    14641 cacttatgca tttaatgggg cagatacacc tgaaaggcga gaacgttttc gtacacttgc
    14701 ggagaattct tggaattacg gattttttga tgaggaggat cggctaacaa gtcaagtcat
    14761 ggcgacccct tttccagtga atttttacgg acaagagtac ctaatggccg gcattggcta
    14821 tgttgcttct tatccagaag caaggggaca aggcgggatc aatcagatca tggaaaagat
    14881 ccttgaagat tgccgtgatc gtaaagtaag cttatcttat ctggcgccat tctcctatcc
    14941 cttctatcgg cgctatggct atgaacaatc atttgataaa atcagttatc aactaaacag
    15001 tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata aagcgtatgg attttgaaga
    15061 tgcaaaagct gcaatcagaa gaatttatga acagatgccg gaaaaccaac gagctggtct
    15121 gaagcgcgaa gactggtggt atacgtataa attcaagcaa aaaggcaatt accagtttgc
    15181 tttgcatttt gatgataatg aggaagcaac gggatatatc gtttatcagc ttgcaacgtc
    15241 ttcctttatt attgcggagt ggggattttt ggatcatgat gctttttgca gccttgttcg
    15301 cttcgtaagt tctcacaatg gtgcttttga aacatttgaa tattcatgcg gctacggcgg
    15361 taatgatcaa aattatctat tagaaaatcc atttgcttct gtaatgatca caccttatat
    15421 gatggcgagg atcgtagata tttctttgtt tttggaaaat tatccattta aaaagagaca
    15481 agctgcattt gccctacaaa tagaagaaga tcattatgcg aaatggaatg aaggaatcgt
    15541 ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa gtagaacaaa caaaactccc
    15601 aatcgttcga ggaagtatcc aacatatcac gcagctatta ttagggtatc gtaaagtgga
    15661 agagttggta ttccaagaaa gaatacaggt gacaaaagaa tatgaagaaa cgttaagctc
    15721 aatcttgcca cagcaacagc caattcttaa tgactatttt tgataaaaaa cgggtgatga
    15781 caatattttt gtcaccaccc ttttgttttt catttggcat ttgaaggaaa atgttaaagt
    15841 aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt ttgttttagg gttttctttt
    15901 ttgtggtctt ggattgcttc tttcgtaatc ttttcccgat tttttttaga tgaatctaag
    15961 cgttctgcgt atactttttc ttttggctta tcattcattg gaaaggacct ccctacatta
    16021 tttcattcta cctctagttt atcatctgtt aaggactagt ggaaacgttt tgcctaactt
    16081 ataagctgat ttttttagca gataagcttg caagtaaaaa acaaacaagc tacaattgat
    16141 ttattgaaat tgttcctgta gctcagtagg atagagcaat cgcctcctaa gcgataggtc
    16201 gctggttcaa ttccagtcag gaacgtataa ataacccctg tatcctttgc ggtacagggg
    16261 tttgttttgt atttgtgcca aaaatgtgcc aaactagtta tcatttttat ataaactatc
    16321 gaacacagta ttgatttttt ctttatcctg atcttcaagc tctcgaacaa tatgaagata
    16381 agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc taagtatatt
    16441 tactccttga taaagtaaaa tagatgcatg tgtatgcctt agtccatgca aagtaaattg
    16501 cttttcaatc cctaattctt ttaatttttt gcgcaaatat ttgctgacgg cgtttgatga
    16561 aactaatcca ttttttaagt tgaaaaaaac aaaattatga gggtttttga tttcaaaatt
    16621 ctcgaataat tctttttgat tgattttaaa cttttttaag agatcaatcg tatgtttatc
    16681 aatggaaatt ttcctatttg aagtttcgtt ttttgttttt ccccattcat ttaatttata
    16741 attccatgtt ttattaatag tgatgatttg atcttcgaaa tcgatatctt cccatgttaa
    16801 tcctagaagt tcagcaaatc gcattccagt ggcaccagca actaacacga gcataggaga
    16861 agagtactgc gcagaaaggt tttcttctac gagtttcatt aaagatttaa attgatcata
    16921 atctaaatat ttatcttctt ttggcttcaa tgaatctttt cctttgatta ctgcttttct
    16981 agttggatca aacggaatta atccttcttc tacggcatct ttcaacgatg ctctaatatg
    17041 gttattaaac ttaataacgg tggattttac atggtctttc gcatacttat ttaaaaagcg
    17101 ttgatatcca actctatcta aatcagaaat caataccgct ggcatatatt tctttatgtt
    17161 cattaacgta tcttcgtatt ttctataagt gataggagat actgttcctt ctttataaag
    17221 ttgcatccaa tcctcaaagt aatctgataa taatagattt ttgcgatccc ctttaaggcc
    17281 tttgttcaag ttatattcta attcgttggc ggcatctttt gcttcgcctt ttgtcttaaa
    17341 tcctgatttt cttatcttcg tgtattttcc gtcatctttt ttgtatgaaa tttcgtattg
    17401 ccaactattt ccacgtttga ctaatcttgc cataattgaa tctactctct ttctctgata
    17461 caataggcac tataaagaag cctactgtat aggtttgttt ttcttagaac acgcttgctt
    17521 tggacggtgg gcgtgttttt tatttattat acagttgctt taaaaatagc tgctttttca
    17581 aatgatacta aaggagagaa gtggatttct atttctccct tttggtttaa cgtgaaatga
    17641 gcagttacat ccatttgttt tcctggagca acagatccca ttgtattttc gttggcatat
    17701 gtctctgatt ttttgtcatc tggtccatat acttccacat ctgtacctac agggatatct
    17761 gaatcgccat cattttttac ggtataagta atttttacta cttgtgcagg ttggttttct
    17821 tcaaattgat ttctttcatc agttagttct acactattta gtgtatattc agcatcacca
    17881 acttttacag tgtcaccaat cttatagaag gtatcgcttt tttcttcttt agaagatgaa
    17941 gcagaggttg aagatttagt tactttttcg ccaccattat cacttgcttt attactatct
    18001 gatccaccat ttaaagcaga accaataatt ataattaaaa ttactgctaa tacccaaaac
    18061 catacttttt tgtaaaaagg tttactaact ttatacattt ttccgtcttg acccataact
    18121 ttttttgcca tttaaatatt cctcacttct tgttataata tatttgcgat ctcagaaatg
    18181 aggtatgagt ccgtgttgca gcacgggctt tttttactgt gcataagagt attttttctt
    18241 gaaatatgac tggcaaacat taaaacattc tgttcttaac ttattattga tagcatagaa
    18301 ttccataaaa ttttccaatt tgaactgaga ttcatctgtt aattcattct caataaagat
    18361 attaagtaga atcataattg caattttatc agcttcagtt tcgaattttg aatgaaaagt
    18421 tgtagagtta tcgtacagta ctgaatattc aaaatgtgaa gcaatgaaat gaccgagctc
    18481 gtgggctaaa tgaaaagctt cagaactatc ttcgtgtagt ttttcgttta aaaatactat
    18541 tcttggtttt ggataataaa aacctggttc ttccatttcc atatagatta actttaaatt
    18601 atactcactc agcatttctt tcaactttaa atacatacaa cccatcactc caactattca
    18661 ttttcctcta aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct
    18721 tttccatcaa aagaaaaaac agtatcgtct tctgataaat ccacatgttt aggggtttct
    18781 ctttcttctc tacctagaag gtagtctaca gagactccaa agtaatcagc taaccgagct
    18841 aactcaatag aattaggagt ttgtcttttc caacttccta aatatccatt cgaatacccg
    18901 aaattcattt cgagctgtct aatggataat cctttttctt ttgctaactc ctttattatt
    18961 tcgtaagtat tcattgataa atcaaccttt ctgaatgctt acaaaaaaag tttagataaa
    19021 taatgtaaaa atagttgact tatatacatt attaatctat actatgtttt gtaaacaagt
    19081 ttaacaacta aaaagacaac aaaaaatact attgattaat aaatgccaac cgccaagaaa
    19141 gctttttaaa tcaaagttta gatgtcttat ttaactatgc atttagtata gattattaat
    19201 ctaataaagt caacgcagtt ttttaaaaag ttgttaaatt tgtttacgaa tataaaagaa
    19261 aggagagaag aatatggaaa aaacagtctc ggcaaaaatc gaagatttga aattagacat
    19321 actgaaacaa gcaaaagtgg cgatggaaca cgcggtagat aaagaagact ctgccatggt
    19381 tgcagccata gcagagattt tagctcacgt ttagtcatcg tttttacggt cttctggaag
    19441 ctgaccatag ataacagcat aatgaatatc taaataagct tgaacaattt cttttggcga
    19501 aattgattcg cctttagtag atacttgtga ttcgtgattt agccatgcaa caactaaatc
    19561 cgcagcgatt tcaaacggcg ttacactttg tgtcagttgc cataaagaat ttcacaatat
    19621 gtatggtcgt ggtaataata ccaaagagca atttgaggaa tttatggaaa ttaaaaaata
    19681 caaaaaatca tttattaaag gaggctaaac aatgaacaag gaaatgagcc aaacaatcaa
    19741 ggtacaaaaa atgatagatg atctaacaca tggaattgat agccaagccg ataaaatcat
    19801 caaggaattg caaggacaaa aagttaagga tgcaaaaatg cttctcaaga ctatcaactt
    19861 tgaaatgaat ccaacaaaaa gaaagctcgc cgatgtgttg gaagaaaagt tggcttccgc
    19921 tatcaacgag caagaattac tatttgaaac cgatactttt aacagctgat gttttataga
    19981 tgacagtttc atccgaatct atatcatcgc cgagagaaaa gaatgttgct tttgacaaaa
    20041 agccgataag acctacaagc ggtcttgatg ttgcaagagt agatacccca atatcacctt
    20101 gcaaggtatt cacgtaaatt tgagaagaat agaacattcc atctttttct tcgatatttg
    20161 acgcatcatc gtatccaacg accaaagtat cggcaaatat tttgattttt tctccatcaa
    20221 gcatagtaat tacaaattct ggtttcataa attttcacct ccttaacaat tatttcagcc
    20281 tgtcacactg ataaggaaat tataccaaag aaaggaatga gaaatatgaa tacaccacaa
    20341 atttttaatt tcgagcaaaa cgaagttcga actattttag taaatgatga accatatttt
    20401 gtaggaaaag atgtagcaga cgttctagga tattcgaatc cgcaaaaagc tattcgagat
    20461 catgtggact tggaggataa gacgcagaac gattcgttca ccgtcaatgg aacagcagtt
    20521 gttttgatta acgagtcagg cctttacagt ttaatcttaa aatcaaaact tccctctgcc
    20581 aaaaaattta aacgttgggt aacgagtgaa gtccttccaa caattagaaa aacaggtagc
    20641 tattcaaacg tacctcaaag ttttgcacaa gcattgcgtt tagcagcaga tttagaagaa
    20701 aagaaccaat tactcgaaca acaaattgcc gagtacgaac caaagattag ctacttagat
    20761 acgattcttt catcgacaga tacggtagct acttctcaaa ttgcagctga ttacggaatg
    20821 tcggcaattg ctctaaacaa attgctaaac gagttaggtg ttcaacataa agttagcgga
    20881 caatggatac tttaccgaaa acatatgaac caaggataca caaaatcgca cacaagtgag
    20941 attccgaaag ccgatggcgg cactaaagtt gtaatgaata ccaaatggac acagaaaggg
    21001 cgagtgttta tttacaactt attaatcgca gagggctatt accctcaaat ggatttagag
    21061 gaaattggtt agaaaggagt tttagtatga ctgacattgc agaaatcact caacgagata
    21121 gagaaaaaat caaagaatat gtcgaaagtt cgaagttctt aacttacacc atgcttgctg
    21181 aaagatttgg aattagcaaa agctacttat ctttaatttt aaacggtaaa aagacttctg
    21241 cagaagcaaa tagaatcata gattcgatta ttactatgta cgaattgtaa agggaggaac
    21301 agctaatgca atatctagaa gcgaaaatcc cagttccaga aggctatgta attatctccc
    21361 aagtggatta tgaggagtta aaaaaagctg atgatactgg tagatggatg acgttgccag
    21421 aagtgctaga acggattaac agaaaatatg attggtttac ttctagagtt ttaaagaacc
    21481 caagatatag aaatattatc gatatcgaaa aaaataaaaa tggatttgtc tattatccag
    21541 ttgaaggaag agacacatat ctatttttaa gaagtaaaac acttgaattt ttagaaacaa
    21601 atttttcgga aatcttaagg aggcaagcgg atgggaaaat ttaatagagc gctagtattc
    21661 agcgcaccgc taatcatcta cgctttagga ctttggggaa gcagacaagc gttgatagga
    21721 acgattgttt acatggtttg gatttttatg gggctggatg aagctgagta caaaacaaaa
    21781 aagccagtcg ggagggactg actaatgaaa aaaagtttaa tgactataaa cgaaaaacaa
    21841 ttgaaagaaa attttaatga tctcatcaaa gaatttgtaa aagaaactgg agaatttcct
    21901 aatcaaattc atctagttgc ggaggggcat agccagtatc aagctgtgaa atttgagatg
    21961 aagaaacaaa tctattgaat ttagcagtac ctctacttgt aagaacattt attgtaatgt
    22021 ttttttcgac taaagattca tcgtatctaa aaaccaacgt agtaattttt gactcatacg
    22081 gtggaatggt aataggtaat gacaacggag gaaattttga tagatcagca ccttttttta
    22141 aagatttacc agaatacgca cgtataccat gtggaagttc gtcatacatt gattttaccg
    22201 ttacagaata attatcacca atcattgtaa aagcatttaa atgatcagga attccagaaa
    22261 tcgaaaactc aataattgta acaggtaaag aactgttatt tgttaacaat acagaatcta
    22321 taatcctgaa tttattttga tggtaaacat ctggtttgga atcactttct ctatctatca
    22381 accattcttc gcattcagcg aagaaatcca attttaaatt aaattttttg ttttttctat
    22441 ctgtatagga gatataaaaa gcaaatgttg aaaatactag agacgtaata ggcaaaataa
    22501 aatctttaat ggttttaaca atgttattcc agtcaaattg tttaataaag tcaataagac
    22561 tcaatttata ccaccaccat tttttatcta cattatatca aagaggagag aagaaataat
    22621 gcaagaatta gtaattttga aaaataaaga agctgtgact acgagtttgc aagtcgcaga
    22681 tagttttgaa aaagaacaca aaaatgtttt gagagatatt gaaaagttaa aagaagatgt
    22741 gctcaatttt gagcagatgt ttgtggaagg taatgaacca gattcatacg gcagaaatcg
    22801 acgagttttc ttcattagta gagatggttt tttcttgttg gctatgggtt ttacaggaaa
    22861 gaaagctatt tacttcaaac aaaaatacat tgaagcattc aacgaaatgg aagatgttat
    22921 tcgtaagaat actgttcctc aaacaattga agatatgatg atctaccaac tagaagaaat
    22981 gaaagatgtt aaaaaagatg tttccatgct taaagatact atgcgaatta gtgggcaaca
    23041 agagtttgaa attaaacaaa aaggaaatat aaaagttatg gaagttctag ggggtaaaga
    23101 aagccgagct tatgaagaaa tcagcaaaaa agtattctca aaattttggt ctgaatttaa
    23161 acgtaccttt tcaatcccaa gatatggcga gttacctcgt aagagattcg atgatgctgt
    23221 ttcatttatt gaaatgtggt taccagaaac tgcgatccgc atggaaatcg atcaactgaa
    23281 cagacaacaa agacttttcg gtgatgaaaa tgaatagagc tgaagcgcta agaatcggga
    23341 cggtaattgc taatcgctgg tggagacaca ataaaccaat catcctaagc caacaacata
    23401 ttgacaagca aaaagcatgg caacaaataa aaaagtgact ccgccggcaa gcaaagagtc
    23461 acaaagaaaa cacatcataa ggagatttta gcatatggaa aaagaacttt ccactctaga
    23521 tcaatatttg actgatccta gttggggcaa atcgaatatc aaggaaacaa ataatcgaaa
    23581 aatcagacga aatcttttga cggatgaaga actagcatgt gatcaagacg atttgggaaa
    23641 ttttgtgagt atttgggatc atgtctatct tatccattta tcgaggaagt ccaaaaaacc
    23701 tgaatatatc tatgtcatcg aagatggctt gattgatgcg ctagaagagt atgacagaga
    23761 taacttgatt gatatctctt attacggatc aggtaagaaa tacattgctg aaatggaggc
    23821 agaatttgat gagtgaaaac aaagggacaa cgacttttga aaaacttttt agtcgcaagt
    23881 taaataaaat actcaagaaa aaaggaaatt ttgattattt atcttgggct cacgcatggg
    23941 agattatgaa aaagaatgat ccacaggcaa cggtaactat taatgagtat aaacactaca
    24001 gagttgtttc tggaactcat caagactttc ttgttgagga atataaacct tttctta
//
LOCUS       DAYLZO010000031        23483 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.031, whole genome shotgun sequence.
ACCESSION   DAYLZO010000031 DAYLZO010000000
VERSION     DAYLZO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.031"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..370)
                     /locus_tag="ACGVJG_001122"
     CDS             complement(<1..370)
                     /locus_tag="ACGVJG_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFJ6557094.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFL"
     gene            complement(375..1022)
                     /locus_tag="ACGVJG_001123"
     CDS             complement(375..1022)
                     /locus_tag="ACGVJG_001123"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFJ6557095.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1107..1739)
                     /locus_tag="ACGVJG_001124"
     CDS             complement(1107..1739)
                     /locus_tag="ACGVJG_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFJ6557096.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(1755..2306)
                     /gene="coaC"
                     /locus_tag="ACGVJG_001125"
     CDS             complement(1755..2306)
                     /gene="coaC"
                     /locus_tag="ACGVJG_001125"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFJ6557097.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2303..3040)
                     /locus_tag="ACGVJG_001126"
     CDS             complement(2303..3040)
                     /locus_tag="ACGVJG_001126"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFJ6557098.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3037..4026)
                     /locus_tag="ACGVJG_001127"
     CDS             complement(3037..4026)
                     /locus_tag="ACGVJG_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFJ6557099.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4284..4826
                     /locus_tag="ACGVJG_001128"
     CDS             4284..4826
                     /locus_tag="ACGVJG_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFJ6557100.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            4843..5928
                     /locus_tag="ACGVJG_001129"
     CDS             4843..5928
                     /locus_tag="ACGVJG_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557101.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            5940..6746
                     /locus_tag="ACGVJG_001130"
     CDS             5940..6746
                     /locus_tag="ACGVJG_001130"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6557102.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            6747..7574
                     /locus_tag="ACGVJG_001131"
     CDS             6747..7574
                     /locus_tag="ACGVJG_001131"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6557103.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            7571..8644
                     /locus_tag="ACGVJG_001132"
     CDS             7571..8644
                     /locus_tag="ACGVJG_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6557104.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9072..11768
                     /locus_tag="ACGVJG_001133"
     CDS             9072..11768
                     /locus_tag="ACGVJG_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFJ6557105.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            11833..12333
                     /locus_tag="ACGVJG_001134"
     CDS             11833..12333
                     /locus_tag="ACGVJG_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFJ6557106.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            12345..12839
                     /locus_tag="ACGVJG_001135"
     CDS             12345..12839
                     /locus_tag="ACGVJG_001135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFJ6557107.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            12860..13669
                     /locus_tag="ACGVJG_001136"
     CDS             12860..13669
                     /locus_tag="ACGVJG_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFJ6557108.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            13666..14487
                     /locus_tag="ACGVJG_001137"
     CDS             13666..14487
                     /locus_tag="ACGVJG_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFJ6557109.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVMGILLSLLGIA"
     gene            14538..16637
                     /locus_tag="ACGVJG_001138"
     CDS             14538..16637
                     /locus_tag="ACGVJG_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFJ6557110.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            16634..17752
                     /locus_tag="ACGVJG_001139"
     CDS             16634..17752
                     /locus_tag="ACGVJG_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFJ6557111.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            17791..18669
                     /locus_tag="ACGVJG_001140"
     CDS             17791..18669
                     /locus_tag="ACGVJG_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFJ6557112.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            18815..19012
                     /locus_tag="ACGVJG_001141"
     CDS             18815..19012
                     /locus_tag="ACGVJG_001141"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFJ6557113.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            18996..19226
                     /locus_tag="ACGVJG_001142"
     CDS             18996..19226
                     /locus_tag="ACGVJG_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557114.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            19471..19662
                     /locus_tag="ACGVJG_001143"
     CDS             19471..19662
                     /locus_tag="ACGVJG_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557115.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(20036..20848)
                     /locus_tag="ACGVJG_001144"
     CDS             complement(20036..20848)
                     /locus_tag="ACGVJG_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557116.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            21011..21118
                     /locus_tag="ACGVJG_001145"
     CDS             21011..21118
                     /locus_tag="ACGVJG_001145"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557117.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            21176..22243
                     /locus_tag="ACGVJG_001146"
     CDS             21176..22243
                     /locus_tag="ACGVJG_001146"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFJ6557118.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            22385..22588
                     /locus_tag="ACGVJG_001147"
     CDS             22385..22588
                     /locus_tag="ACGVJG_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFJ6557119.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            22648..>23483
                     /locus_tag="ACGVJG_001148"
     CDS             22648..>23483
                     /locus_tag="ACGVJG_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6557120.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKL"
BASE COUNT         7313 a         3962 c         5020 g         7188 t
ORIGIN      
        1 tcaaaaaaac ttctgagatt ccagcagcag aaacatagtc tagcgtatca gggagaagga
       61 ttgcacgatc ggcaggcatc acagcatatt cggcataacc acctagattg actaatccgt
      121 atacttttgt cccaggagta aaagtggaga tatcactttt attctcaact acctctccag
      181 ctacttcgac tcccaaaata gggtagggag gtttcaaaga gactgattct cttttcataa
      241 tatctgttcg attgacagct gctgtatgga ctttgatcag taattcgcct tgttttggtt
      301 taggaacagg cacttcaatt tgttctaata catctacaga accaggatgc ttgattgaaa
      361 tggcacgcat ggatctattc ctcctttgcc cattcttgga atgctgtatt caactctttg
      421 ctaccttcag aaagtactag agtcgtacga gaaatatccg tttcttccac tagagcggca
      481 agtacagcag caactgtaga gcgagtgatc gtttttgatg ccttctcatt aggcttagtt
      541 aattgaatgg aagtaacttc ttcatcgtta gtcaatgtga ctggttgaag aatgacaaaa
      601 tctaagttag ttcgtttcag ccattcatct gcataatgtt tagtaatata ataatctgtc
      661 atggactcag gccattttgc tggttcatct gcataaacag cactgaccat cagatagcga
      721 gagacatgcg cattttcagc tgcgatcact gttttgattg cgccatccaa gtctacttgc
      781 agaagattct ttccttgaga gccggcagca aaaatcaata catcgatgcc tttgaatgct
      841 tcggtcattt tttccacgga ccaagttaaa tcaaaagaga catacgaaac attctctttt
      901 tttactaatg tttgggaagc aacatctctc acccctgcaa aaatttcatg ggaacttgga
      961 gctaaatctt ttatcaggtg tcggccaatt tgtccgtttg cacctacaac aaaaattttc
     1021 atttggatca tcctttcgac gtcaaatctt tccattcaat agtaacacca aaagagaaaa
     1081 aaatcagaga tcggctacga tgactatcag ttagtaagag aagggtgctt gaagtatctt
     1141 aacaagacat tgcatactgc tgcagtcgca agacctgcga cgattccttc aggtatacca
     1201 cttgtaaaaa taaccgctag aatagctgaa taaacagcat ttaaatttgt tccgatgact
     1261 ttggcataat cttgctggaa taaaaagtag atcatattca tcactaaaat cgtattggtc
     1321 aatgaaccgg aaagtccagc taagaataaa gaaacggaat ctgctttttg tttcattagt
     1381 ttgcgcagac ctttataaac gaaataagga accaccccaa tcaagatgcg aggaataaac
     1441 gcgatcagca aggctttcca gcttccatgt tctgttccga tgactgggat aaacggagaa
     1501 aagacaaaag acaaaggtgt ttggataact gtgctacgga tcatgctgat cagtccaaat
     1561 gtaccgccaa gaaaagcacc tagtcttggt cctaaaacga tcgaagcaat gattacagga
     1621 atatgcatgg ttgttgcatt gattggaccg atagggataa aacctaaagg cgtaacagct
     1681 aataaaatca aaatagctaa aaacatcgca gttaatgtaa agttttttgt atttttcata
     1741 agtactcctt ttttctaagc atttctttca ttaagagtca gcatgacctg ttcgatgatc
     1801 gtctgaatat cagctaaggc accttttcca aaatctccac aagctaaaag agattctctt
     1861 ggttcaattt cctgatagcc gacagatctc aatgtttcca gattacgctg agtgatcggg
     1921 ttttgataca tattcgtgtt catggcaggg gcaatcaatt ttggtgttcc cactggaagc
     1981 gccattgcga ctgttgaaag aagatcgtct gcaatgccgt ttgctagctt gccgataatg
     2041 tttgctgtag caggcgcgat gagaaacaag tcagcttgtt ttgctagttc gatatgattg
     2101 attacctgcg gatttgattc ttgcatgacg tctgtatgga ctgcgcgctt cgacagagat
     2161 tgcaaggtta atggcgtgat aaaatgtgtg ctgcttttag acataatcac atctacctgg
     2221 gcacctaatt tgacgaattg acttgtaaga tcagcgcttt tgtaagcaga aatacttcca
     2281 gtaacgccta gtaagatctt tttcattttg attcctccag tctaattttc tcagtaatca
     2341 atgcagcaat ctcagattta ctttgagctt cttccactgt tccatcttta gataagagat
     2401 agccgtggtg tgtctcgcca tggatttccg tcaaatcatt tgcgaaaata aaatcagttt
     2461 tgttatttat cagactattt tttgcgactg ccagaagaga ttcctttggt acatctacta
     2521 atagtttgaa accaatgatt tttgcttttg gctgccattc acgtagataa gaaatgattt
     2581 ttggtgtttt cttcaaaacg agcacaagat gctcggtgtt agaagagatc tttgaaacag
     2641 tggtatctgt ctgttcattt tccgcaaacc aattgtccag ttcttcttgc gttgaagaag
     2701 tgggtaggtt ttttgccagc tgttcttctg aaaaactaaa agcaggagta aaatcactga
     2761 ctgccatact gtggataatc gcatcgtagt gttctttttt cgttagttct tctaattgta
     2821 aaaataagtc cagtgcagat tcgatcgtat aaatcgttat attggagtgt cttttcggtt
     2881 tcagtgcttg tttcgtagtc acgtaatcga ttgtggtatc cggattggca gctaaataat
     2941 cagctaatgc ttgccctaat cgtcctgtcg aatgatttgt aatagcacgt acttgatcga
     3001 ttttttcagt cgttcctcca gctgtgatta gaatcttcat ttatggtcac ctacatttac
     3061 tagtgttctt ccttggtgtg ttccagcaag tagtttagat gctgtttcag gcaattgatc
     3121 cagagtaatc tcttggacga ccagttcatc tgcaatctgc aaatccgcca tctgttgcca
     3181 taaagaaaga cgttgatcta ttggtacatt gacagaatca attccaatca actggatgtt
     3241 tcgcagaata aaaggcaaga cagtcgcgtt tagtttgatt cccccagcat tgccacataa
     3301 agctactgca ccgttataag agatcaatgg aagaagactg cttagctgct cgccacctac
     3361 tgtgtcgatg acatagtcga tttgctgttt tcctaaagct tttgttgtct ctggcagaaa
     3421 ttcatcaggt cgtacgactt gcaaagcacc tagacttttt agccatgtga cagactcttc
     3481 ttttctggaa aaggcaatga ttgactggaa tcctagttta tgtaacaggg ctatcgctgt
     3541 actgcctaca ccgccagaag caccagtaac gacaactttt ttatcggagg tcataccttg
     3601 acggattaaa gcgttcacac acaacatagc agtaaaccct gctgtaccaa ggatcatcgc
     3661 ttgacgcagc gtcatatttt ttggtagagg aactaaccag tctcctggta ctttttgata
     3721 ttggctataa ccgcctggat gggtgacgcc aagcccaaaa ccagtagcaa gtacaagatc
     3781 ttcttttgaa aaacgtggat tgttactttg gacgacagtt ccggataaat caatccctgg
     3841 agtcatcgga tatgttcgta tcacctgacc agactcgctc attgccaatg catctttata
     3901 attgacatca gaataggcaa tttttaccaa gacttcattt tcagccaact cagtaaatgg
     3961 cagatcactg attgctgtat ggaaaacagg ttcctttatt acctgaaaag atcgaaaagc
     4021 gttcattcgt gagacactcc ttttaaaatt ttcttttatc ataccataaa gcctactctt
     4081 ttattcttgt tcttccgtaa atttagacaa aaattaggtg aaaaataaag aatcaaacaa
     4141 ctagaattta ttccaaaaga taaagaaata ttcagcaaaa aaaattttac aaagggttgc
     4201 ctttttcaat aaatttcgta tactaatacg agtggtagta aacaggaagt ttactattag
     4261 aaaacttcga ttcggaggaa attatggaaa ttggtgagaa gttaagaaat ttgcgcatcc
     4321 aaaagaattt gacacaagaa gaactaggcg aacgcacaga tttgtcaaaa gggtatattt
     4381 cccagttgga acgagattta agttcaccat caatggaaac attcttttct attcttgaag
     4441 tcttaggtgt gactccagaa gaattttttc atcaagaagc agcgaatctg caagtggttt
     4501 attcaaaaga agatcatacg gtttatttcg atgaagaaaa cgggtatgaa ttggaatggc
     4561 tcgtggctga ttcgaatgaa aaagaaatgg agcctgtctt gctgactttt gaaggtgcag
     4621 gagaatataa gacgtttgaa ccttcattat ctgaaacgtt tatttttgta ttagaagggg
     4681 aattagagtt aagcttagga gagacggtct atgtggcaaa agcaggacag tcgatctatt
     4741 atcaagcgac aaaacaacat caattaagga atcatctcaa aaaacgaaca aaagtattga
     4801 ttgttgcaac gcaatcatat ttgtaatata gggggtaaaa acgtggggaa accaatcatt
     4861 tcatttgatc aagtagtgaa acgctatgat gatgaaacag tattaaaaaa agtaagtttt
     4921 gagatcgaac aaggaaaatt ttatacactg cttggtcctt ccggctgtgg taaaacaacg
     4981 atcctacgta ttatagctgg attcaccgaa gctacagaag gggacatcta ttttgaaggg
     5041 aaacggttga atgatttgcc agctaataag cgacaagtaa atacagtctt ccaagattat
     5101 gcactttttc ctcatatgaa tgtttttgat aacgttgctt ttggattgaa gatcaagaaa
     5161 atgtccaaat cagatatcga aaagaaggta aaagatgctt tgcgtatggt ccagcttcct
     5221 ggatacgaaa aacgggagat tagtgaaatg tctggtggtc aaagacagcg tgtagcgatt
     5281 gcacgagcaa ttgtaaatga accgaaagta ttgctattag atgaaccgtt atcggcgctt
     5341 gatttgaaac tacgtacaga tatgcagtat gaactgcggg atctgcaaca acgattagga
     5401 atcacgttta tttttgttac tcatgaccaa gaagaagcgt tggcaatgag tgatgagatt
     5461 tttgtcatga ataaagggaa aatcgtccaa agtggtacac ccgtagatat ttatgatgag
     5521 ccaatcaatc atttcgttgc tgattttgtt ggggagagca atatcgtgga cggtgtaatg
     5581 atcgaagata atctagttga gtttgttggt aagcaatttg aatgtgtcga tggcgggatg
     5641 cgtctaaatg aaccagttga agttgtttta cgcccagagg atttgacgat cacgacacca
     5701 gataaaggaa aactagtcgt aacggtcgat actcagctgt ttcgtggtgt ccattatgaa
     5761 atcatctgtt atgatgaaca gcaaaatgaa tggatggtcc attcaacgaa aaaagctaaa
     5821 gaaggaagca aagtaggatt ggcctttgag ccagaagata tccatgtcat gcgtttcaac
     5881 gagtctgaag aagaattcga tgcacgtctt gacagctacg aagaataggg gggacaagaa
     5941 tgaaaacaat gcgcaggatc tattccgtcc cttatatttt gtggctcggt ttattcgtga
     6001 tcgcgcctgt attaatgatc atttatcaat ctttttttga tatgaatggg caattcacgc
     6061 tgaataatta tctatcttat tttacgtcgg ggacatcgct ttctatgacg atcaattctg
     6121 tatggtatgc atttttgatc acgtttttca cgctgatcat cagttacccg actgcttatt
     6181 ttttaacaaa actgaagcac aagcaattat ggctgatgct gattattttg cctacatggg
     6241 tgaatctgct gcttaaagct tacgcattta ttgggatttt cagtgtgcac ggcagcgtga
     6301 atcatttctt tgattttatc gggatcgggc cgaaacagat attattcaca gacttcagtt
     6361 ttctgttcgt ggcaacgtat atcgagattc catttatgat tatgccaatt ttcaatgcat
     6421 tggaagagat gaatccttct ttgatcagtg ctagtagaga tttaggcgcg aatagctttg
     6481 agaccttccg acgagtgatt tttccgttat cattaaatgg tgtgaaaagt ggtgtccaag
     6541 cagtatttat tccttctctt tctttattca tgctgactcg tctgattggc ggaaatcggg
     6601 tgatcacttt aggaacggct atcgaacagc atttcttggt tacacaaaac tgggggatgg
     6661 gttctacaat cggtgtgatt ttaatcattg ctatgttcat cgtgatgctc ctgacaggcg
     6721 agaagaaaaa aggaggtcgc tcataaatga aaaaatttaa atggtcgtat ctttatttag
     6781 ggatcgtctt tttattctta tatgcaccga tcttctatct gattttttat tccttcaatg
     6841 aaggaggaac gatgaacagc tttacaggat ttacttggga aaattatgaa gctgtttttg
     6901 aagatacacg tttgatcatt atcgtattga atacgttcat gctggctttc ttatctgcat
     6961 tgatcgccac gatcatcggg acatttgggg ctatgggaat ctattacacg aaaaaacgac
     7021 gtgcacggac agcgttgttg agcatgaata acatcttatt agtctctcct gatgttatta
     7081 tcggtgctag tttcttgatt ttctttacac tactaggatt cggattaggt tttcagagtg
     7141 tattgctttc ccatattgct ttcagcattc caatcgtcgt cttgatggtc ttgccaaaat
     7201 tgcaggaaat gaacgattca atggtcgatg cggctagaga ccttggtgct aataatttcc
     7261 aagtagtcaa aaatatcatt ttgccttatc tttctccagg aatcatagct ggatatttta
     7321 tggcatttac ctattctctt gatgattttg cggttacatt tttcgtcaca ggaaatggct
     7381 ttacgacgct ctcagtagag atctattctc gtgcaagaca aggaatcagt ttagaaatca
     7441 atgcgctaag tgcgttagtt ttcttgttct ccatgatatt ggtcattgga tactacttta
     7501 ttagtaaaga aaaccgtcca aaacggctga agaaaaacag aagagtacgc gagggggtgg
     7561 cacggctgcg atgaaaaaat tacaatctct gtttttaggt attctcatca ttattgcggt
     7621 gctgctgctt ggtgttcgtc aacttgaaaa aacaagtggc atgagtggag caaaggtact
     7681 taccatctat aactggggag attatatcga tcctagtttg atcacgaaat ttgaaaaaga
     7741 atatggatac aaagtgaatt atgaaacatt tgattctaat gaagcgatgt tcacaaaaat
     7801 ccagcaaggt gggacaaact acgacatcgc tataccaagt gaatatatga tccaaaaaat
     7861 gatcaaagaa aaactagtac ttccattaga tcattcgaag atcaaaggat taaataatat
     7921 tgatccacga tttttagact tggattttga tccagataat cagtattcga ttccatattt
     7981 ttggggaact ttagggatcg tatataacga taaattcttt gatgcaaatc aaattaagca
     8041 ttgggatgat ttgtggaagc cggaattaaa agacagtttg atgctgattg acggtgcacg
     8101 agaagttatg gggctttctt tgaatagttt aggctattct ttgaatagta agaatatggc
     8161 acagctgaca gaagcagcaa ataaattgaa tcatttaaca ccaaatgtca aagcaatcgt
     8221 ggcagatgag atcaaaatgt atatgatcaa tgaagaagcc tccgtggcag tgacattttc
     8281 tggcgaagct gccgatatga tgtgggaaaa tgaacattta cattatgtga tcccaccaga
     8341 aggatccaat ctttggtttg ataatatcgt tatgccaaaa accgtgaaaa ataaagaagg
     8401 tgcctatgat ttcatcaact ttatgctaga accggaaaat gcggcacaaa atgcagagta
     8461 tattggttat tctacaccaa atgaagaagc aaaagcgtta ttgccaaaag acatttcgtc
     8521 ggacgaacaa ttctatccat ctgatgacac gatctcccat cttgaagtct acaaagatct
     8581 aggatcgaaa tacctaggca tctataatga cctattctta gaattcaaga tgtataggag
     8641 ataatggcaa aactcagtaa gaatggggac ttatcaacgt tctaaagagc taaaaatcgt
     8701 cctttttcta tctgagtttt ccaatcttct accaaaagcg gatgaaacgc cgcttttttt
     8761 gtgttatttt attttttcta ccacgttttt ctaccagaga aaagtggaca aaaccattgt
     8821 aaaatagggc ccgagacggg ctagtaaatc taagattgaa aaagttacag aaggataaag
     8881 taattggaaa ctatgcaaat atacttaacc cttctgtaat ctattaataa aactaatata
     8941 tgaatccagt tcaatatttg tagctatgaa aaaacataaa aatcatatta gataaaaact
     9001 aaattggtag ataaattatt ttgtcagata tctgaggcaa atattcgact atttgtgttt
     9061 caaagtaaaa attggaatac aaatatagaa tagacatatt gcagccgata gtttattatg
     9121 cttttagaca cattaaactg gaggaaaaaa tgaaaaagag cgctaaagat cgagtttttg
     9181 atgagctgat acgactaaca gggaatatgt cttctgtcac tgccgagaaa atttcgaaga
     9241 atctaggtat ctctcgtcag aatgccagcc attatttgac gagattagtg gaagataaaa
     9301 aggtcgagaa attaccggga aagcctgttt tgtggaagcc tttagatgaa tatgcggttg
     9361 tagataatac ggagcaaata aacgaagcct ttcattctgt agttggtcat gacggaagtc
     9421 tgagagaggt cattcaaaaa tgtatagccg ctgtcaaata tccgcctaac ggactcagtg
     9481 tattaataaa tggagccacg ggtgttggaa aaagtttttt agccacaaaa atttttgaat
     9541 acgctatcca tgagcaaatt attgaaaaag atgcaccatt tgctatctta aactgtgccg
     9601 attatgcgga caatcccgaa ctgctttcgg caacactgtt tggatacaag aagggggcct
     9661 ttacgggggc tgaaaaggat accgatggtt tattggcaac ggccaacaac ggctatcttt
     9721 ttctcgatga ggtccatcgc ctatcgaaag aaaatcaaga aaagctcttt ctatttattg
     9781 atactggaaa ctatcgtcca gtcggtgaaa atgtaaattg gcattctgct aaggttcgtt
     9841 ttattttcgc cacgacggaa aaaggtgaaa attatttatt agacaccttt gatcggcgta
     9901 ttcaaattag tgttatgttg ccaacttttg aagatcgccc cattcgagaa cgattagagc
     9961 tgattcaatt attttttcaa aatgaagcgg atgttttaca aaaagatatt attgtttcaa
    10021 gagaagcctt gtctttaatg ttatctcatc cctttagtgg gaatattggt aagctgaaga
    10081 atatcattaa aatcagttgt gcggatgtgt atagtcgaac acaagaagaa ccattaacga
    10141 ttgggaaaaa tgagatcttg attcagttaa atatgctgga atccgaagta gaaagcttgc
    10201 cgattgctag tttattgata aagaaaaatc aaaagataaa aacggatcga atcaaaccgt
    10261 ttgattatga ttcgattttg gaaaaaatta ctcagctgat aaatgaaggt aatttcaatg
    10321 aaaatttttc ttcgataaag aatgaagtac agcatttggt gcatcacatt gataaggatt
    10381 cttttttatc aatgagtaga aaattgatga ttcagatgtt tagaaaggtc tgggtatccg
    10441 ttttaggaaa aacgtacgga ttgacgacga gtggacctgt tgcagaaatt attagcagat
    10501 cctatgtcat ttccccaagc tataagacag acttaaccgg gataaatcgt acattagctg
    10561 caaatctacc tcggacgact tacttatgta atcagttctt ggagcattta ccaccgctct
    10621 cagaaaagga cgagaacttt ctaagactgc ttattgtcgt gactttatct gattatgtag
    10681 atgaatccat cgagctgaag ggcctgttgc ttgctcacgg agatagtacg gctagcagca
    10741 ttcaagcagt agtgaatcaa ttatgcggca attatgtttt tgaagcgttg gatatgccag
    10801 tagagagtaa tgtgtcagag attgtcgaat cgacaaggaa tttcgtgaaa cggcaagtga
    10861 atacagatag tctagtgctg atcgtagata tgggctcttt aaatcaaatt tacacacaga
    10921 tcaagaacga tttaaaaggg gagttacttc ttctgaataa tttgacgact tccattgcac
    10981 ttgatattgg cttgaagatg acgatgaatg cgccatttaa acagattgcg gaggatgcag
    11041 cagacaagta ttcaatcaat gttcaatact ttgaaggctt ttctcaaagt agcaacatta
    11101 tcatttcctg tatgtcgggt ttggggattt cggacaaatt gaaagatgtt tttcgtcatc
    11161 atttatcgga tgaatccatc gaagttttca cgaaggacta cagagaacta cgtgaattaa
    11221 tcgaccaaaa tgatgaaact tattttgaca agacaaaatt ggtcatcacg acttcggatc
    11281 ttccacgttc cttttcaatt cctaatatta atgtttatga catcttagac ggtgatggag
    11341 cagccaattt ggagcatatt ttgagtcctg aaatgaagcc ggcagaattt aaccgcttga
    11401 ttcaggagct agtaaaattc ttttcgttgg aaggaattgc ggaccgtttg aactttctga
    11461 acccaacgat cgtgatgagt gaagtggaaa cagtcatttc taaatatgaa aattactatc
    11521 atttcacttt aaatggccgt gtaaaactaa atctctatat gcatacggca ttgatgatgg
    11581 aacgtttatt cttggctcgg acagaaaaat ctgaagaacc gaattatgat ttgaaaccag
    11641 atgaagatga attttattat gtttctagaa gtatttttca atcaatggag atgaaatata
    11701 attttaaagt caatgcttat gagctttcgc tgctctatga attattagga ccgtatattc
    11761 aaaaataaag tgtctaaaaa agtgtctaat ctgttttttt taggttagac acttttttta
    11821 atgtgaatta acgtgattaa accaatgttt ttaaaagttg gcacaggaat tgcttatata
    11881 aaagtgaaca gaaagaaagg aattaaccat atgaagaaaa ttctaatagc gactcacggt
    11941 cacttagctt caggctttct tagcagtatt caattactga cggggaaggt tcaagagatt
    12001 acggtgatta atgcttatgt ggatgagtgt gattttgaac aggagttgaa tgtatttgta
    12061 aaccaggtga acgagcagga tcagattttt gtttttaccg atttatttgg cggaagtgtc
    12121 aatcagaaga ttaccaaaac ctttttggag aaagagattg ctgtaactat tttgtctggc
    12181 tttaatctgc caattctgct cgaaatagta ttggcaacca aggaattaac aaaggaggat
    12241 attcaggaac tggtaaataa atgccaacag gaattaaaga tgaccattct tgatgaggta
    12301 catgtagtaa atgaagagga agattttttt taggaggaag aaaaatgatt aaagcagtgc
    12361 gaattgatga acgattaatt catggacaag tagcgatgac ttggacaaag agtctaaatt
    12421 taaccgggtt agtagtagca agcgatgagg cggctaccaa cgatattcaa aagatgacat
    12481 tgaagatggc tgcaccatcg aatgtcaaag tcattattaa aacagttgat gacgcgattc
    12541 atttgttaaa agatccgcgg gcgaaggaca tgcgcttgat ggttttagtc cctaatgtca
    12601 aagatgcggt aaaagttgcg aaagctttcc caacggaaat cgaattaatg aacgtgggga
    12661 atgctgggaa aatgacaccg ccatcggaag ataagaaaac cttatcaaag gaagtgatgt
    12721 taacgacgtc agaaattcaa gacttaaaag aattagttga actttatcca gataccgttt
    12781 ttcagcctac accggcgatg gaaaaacgtt cagctatctc tattttgagt gatttttaga
    12841 aaaaggaagg agaaaaatta tgtatgatgc atttgtagtc gctttggccg tttttattgg
    12901 agttgctgga catgaaatgt tcgggatgtc catgctgagt cgtccgattg ttgttgctcc
    12961 gttagcagga gcgttgatgg gggatttgca gacggggttg attgtcgggg cgtcactaga
    13021 agccatcttt atgggtgtcg tcaatatcgg aatctctagt acggcggagc cttctttggc
    13081 tgctgggctg gctacagcgt tcgcaatcaa aatgggagga aacgtggatg cgattattcc
    13141 cattgtcttt ccactagcag tattaggatt acagttaatg aatatgattt tttcttttgt
    13201 atgtggaccg atggctgcta aatttttcaa atttgcaaaa tctggtgatc aaaaaggaat
    13261 catcaatctg cacttcttta tgtggtttgt ccactatgcg ctttatgctc ttattccatt
    13321 ctttgccgtt ttatttggag cagatgttgt caaaagcgtc ttggataata ttccagaggt
    13381 catcatgaac ggattaacag tcgctggaaa tctgctgccg gcagttggga tggcgatgct
    13441 gttacggatg ctttggagta aaaatattgc tgtgtggttt ttctttgggg ccgttgttat
    13501 ggcgtatctc gagttaccat tgattgcaat tgccgcagtt ggaacgatta ttgcaattgt
    13561 tatagcccaa agagatctac aactaaaaaa tatatctgtt catgcagttc aaagcgatgc
    13621 tgctgtaaat gcaacgcttg accaagaaga ggaggatttc tttcaatgaa attagctgat
    13681 ggaatttcta aagatgagaa aaaattggtt cggaagatgt tttggcgctc gatgactatg
    13741 tatgcttcgg tgaatccggt gacgatgggc ggcggtggtt tcgcctattc tatgatgccc
    13801 ttcattaatc atttctacaa aagtgaagaa gatcgagctg cggcgctgga acgtcataca
    13861 cagtattttt cgacgaccat tccttgcgcg tcctttgtca tggggattgc ggcatcgatg
    13921 gagaaggaaa attctgaaaa agctggtttt gacgtggaaa gtatcaacgc gatcaaaacg
    13981 agtttgatgg gaccattggc tggaattggc gattcattgt tctggggagt ttggcgtgtc
    14041 gttaccgcgg gactagcgat caacttagcc aatgccggaa atattttggc accaattatc
    14101 tttttagtta tgttcaatat acctaattac ttgatgcgat actatggcgg cgttttaggc
    14161 tattcattag gctctaaata tattgaaaag ctgtatgcga acgggttaat tgaaattctg
    14221 acaaaagctg ctagtatctt gggtctgatc atggtaggtg caatgacgat cagtatggtc
    14281 aaattcaata ccgttttgag cttttcaatg aagggcgaag aactaatgaa tgtccaagaa
    14341 atcctcgata gtatttttaa aggaattgta ccgttattgg taacattgag ctgtttcaaa
    14401 ttactagata aaaaagtcgg cgtcatcaaa attatttttg gaatggtcgt catgggaatt
    14461 ttattgtcat tacttggaat cgcatagtag gaagctgcag ctactaggct gcagtttttt
    14521 tagcaaggag ggaaaagatg actattaatg aagtaattta ttcacccaaa cacccatttt
    14581 ttaatgctga gttaaacgac ggaaatgtga ctacttcaaa cgaatacagc tactatcgga
    14641 atattcctga tgtaaaggga ttgaccttcg atgtaagtaa tcaaaagaca ttagcaaacc
    14701 ttgatgtttg cgggacgatt caaacgttga ccttttatcg agaaaattta ctaactgagg
    14761 aaaaaccagg tgtttgggtc aataaacagc tctctcaaac caattcgtta tccttaaagg
    14821 ttgaagtaaa cgggattgtc taccatttgt cagaatcgga tcatcgtatt gaagtcgatg
    14881 tgattcaaga ctgtttgcct agatatattc atcattattc gacgtttaag gtcattgtag
    14941 tatcctttgc tcccatactg gctaataagc gtttaagtat gcttgttcaa caggtttaca
    15001 ttgtgaatga aacacacgaa agccttcaag tttcagtgaa agatgtgcct tcgtatcaag
    15061 agaagtattc ggatcagcaa aatgtgttga tttctcaaaa aggaaataag gacacgatcg
    15121 cacaacagga agtggcaagc ttcgccattg cttttgtgga cccaaatgct tttgaagaag
    15181 taatgacttt tcaggaatct gatatcgaaa aatggctgaa ggaaacattg aactattttg
    15241 ctcagctatt tgggcagctg tcaatgccaa acgatagagt tgtccatctc tatcgtcgtg
    15301 cactttatca aagcttttct agtttcggta tgaatcgaca gggacaggtc gtcggcagca
    15361 attggggcag ctatcctgct actaatcgaa tttggaataa ggatatgtat tacgcatcat
    15421 tgccattcac catgtttgaa ccggagttat gtcaaaaaac ggttctatgg tttgatcaat
    15481 atggcgttaa gtttcctggt tcaaaattta caggaggtat caatcattct ctgagtaatt
    15541 cgttatccag tgcgttactg gctagtcttt attttgaaca tactggggat acaaaattct
    15601 ttgagcggtg tccgcaggtt ctactgaatg cttcaagaat tattgaggac atattagctc
    15661 aacgtcggtc aggggaacca atgctttttc catctgtttg gttatcagac gcctttgcac
    15721 ttggaaagta tcataccggc agcaatcttt gtctttggaa ggcctgtatt ggacttgcag
    15781 aaatttttga agccctagaa caactgagtc ttagcaagcg atacaaaaac attgcctgta
    15841 aactaaagga atcaatttgt aaacaaatga ctatcgatgg tccttttggt gagcaatttt
    15901 tggagggaat cggtgatgag gagaaaacaa cctattcagt gaagcactat caaaaaccga
    15961 ttcttgagca agggttaatc tttttaagtg atgtgattgt ggatggaaaa attaatttat
    16021 tgatgcatga tggagaagaa tcagacacta cgttgatgcc tttttatcaa tttttagata
    16081 ataaagatca tttatatcag aacaccatgg ccttttctgc tagttctttc aatccaacct
    16141 atagcgagga aatcaaaggc atcacttggg gattagaatc cggcgccact tttcctggct
    16201 ttatcactgt tttaatgagc gagctgcaca accctcaagc tttcgtaacc cgattagaag
    16261 aactaatttg tttggcagat ttagacggtt cgtggtggtg gtggccgtat cgcttagagg
    16321 ctcaacaagg agaagtcgtg cgagactttg gctgcggcaa atgcggctgg gcatcgggtc
    16381 tttttgtcag cctaatgatc agtcaatatt tcggattgaa attcaaaaaa ggagtattgc
    16441 aaattgatcc tgttgataat ttaacgtttt cttggaagaa tttgaagttc ggtcaagcct
    16501 tcatttcgat tgaatgtacg caatccgagt tgagtatttc aaatttgtca gaaaagccat
    16561 tgaaaataag tgatacgaaa aaaattcttc acgtagaaaa gggaaaaacg attcacattc
    16621 aaaggagaaa aagatgagta tactaaaaaa tattacccaa tttaaaaaaa tccagagtcg
    16681 agcaatcact cccgaaaatc caacaggcga gaaaggtaaa gctgcgatgt tgccaagcga
    16741 gctaggacca agccgcaaag gtcgtggcgc aattccgctg acttctggtg aagaaactgt
    16801 gattgcggat attactggaa cgggaataat tcgtcatatg tggatgacga ttcgtgatcg
    16861 aacagaattt ggcagttttg ttttacgtga tttaatctta agaatttatt gggatgggtc
    16921 ggatagccct gctgttgaaa gtcctttagg ggatttcttt tgtaatgggt ttggagagcg
    16981 ttgcgaggtg aactcgttgc cgatagtggt taatcctaca gggggcttta attcctattt
    17041 cgaaatgcct ttccaaacat ccgctaaaat tacgatcgag aatttacatc cgaaggatat
    17101 gtcctcattt ttttatacga taaattacgc tttggtggac gaattggaag aaaatagcct
    17161 atattttcac gcaaattggc atcgggaacg cattaccaaa ttacaggagg attataagtt
    17221 aattgatcga ttaaagggtc aaggctatta cgtgggtact taccttgcgc ttacctgcct
    17281 agaacgttat tggtggggcg aaggcgagtt taaattctac attgatgggg atagtgagta
    17341 tccgaccgta acctcaacag gttcagaaga ttattttggc ggggcgtggg cctttcatga
    17401 gtacgatgag ttcggacgtc cacatgcgaa aaactattca acagcctttt tagggtatcc
    17461 ttaccaatca aatagagatg ccacaagaga tcgttttgaa acgggaaaat tgaatgccgt
    17521 tcatgccttt ggtgacgacg caatgccacg acatggtctt tatcgttggc atcttcaaga
    17581 ccccatctgt tttaccgaag atttatcggt cactttacaa caaatcggca acgatgatgt
    17641 tcgcttattt gagcggcaag acgatgttgc tagtgtcgct tactggtatt caacagaagc
    17701 ttcaggaaat actaaacgtc atttcactga tcgagaacgc ttgccgcgtt aatgaagtgt
    17761 ttttcacata ttcgaaggga ggaacctatg atgatatatg gggcaattga agctggaggg
    17821 actaaatttg tctgtgcagt cggtactgag gaattaacga ttttggaaag agtcagtttt
    17881 cccacaacaa ctccagttga aacaatgaag caggtcattg atttttttaa accttttaag
    17941 gaacaattgg caggcatcgg ggtcggctgc tttggtccaa ttgatattca atgcaaatca
    18001 acgagtttcg gtcatatcac atcaacacca aaacttgctt ggcaaaattt caattttgta
    18061 ggaacgctaa agcaatattt caatattccg attgaatgga caacagatgt caatgcggct
    18121 tgctatggtg aatatgttgc tggttctggc aaagggttag caggtgttgc atacttcact
    18181 gtgggaacag gtatcggagg tggtgccttg ttcaatggga ctttcgtgga agggttcagt
    18241 catccggaaa tggggcatat gttgatcaag cgacatccaa aggacaattt ttcaggaaac
    18301 tgtcccttcc accaagattg tttagagggg atggcagcag gaccagcgat tgaaaaacgg
    18361 ttgggtgtaa aaggccagaa tttattggct gacgattcgt tttggcagat tgaagccttt
    18421 taccttgccc aatgtgcata caatacaaca ctgatgtttt cgccagatag aattattttt
    18481 ggcggtggtg taatgaaaca agaacacatg aagaaaaaag ttcaggataa atttgttgag
    18541 cttataaatg gatatgttga aataccacca attgatagct acattattac gcccgaatta
    18601 ggtgataatg ccggaataat cggcggatta gcattggcca gaaaagcggt aagaaataag
    18661 caaccataat taatgaatgt ttcattgata ctcatgtcaa aagtatttaa ccgagtaatc
    18721 aaatgatcaa atgaagttgg ataaagcgaa aatgaattca aataaaaagg ctctaagtca
    18781 aatcgtgttg ttttcattaa atcactgaaa ggaaatgctt ctgcgtttcc ttttttcttt
    18841 ttgttgtgcg ctgtatacct tgaggatatt cactggtggc gtacacttaa atagtaatat
    18901 attgtgttac tttatgtctg tggtaatagt tatagttgct taccaatgta tgttcatttt
    18961 tcgaaatgtt gacttcataa tccttggctt aatgtttgaa tttttttggt gatagatttt
    19021 ttcagccgta taaaataaat cgtaattcta ttatagagag ttgtgattat aaaaagatag
    19081 caagaaaaaa aaagaaagtt tctatacatt ttaaatattt tattcctatt tctattgcta
    19141 aaaaagtaga aattctatat ttacctttaa ctctcgcttc tcttgcaaat gtagttagca
    19201 ttttaatcga taataaactc aggtagttat attaccttga aatgattgat aattttgtca
    19261 cttctatttt ttttgcaata aattttttat tttagtgaaa aataagaaac tatttctttg
    19321 aaggcgataa gttagaaaaa tattattgta gtcttttctt tattaggaat catcactgtt
    19381 gtatcttatt accttttctg tttttcttat tagagaaaaa taatttgaga ggtagatagt
    19441 ttttattgga ggaagtaatt atgtatattt ttgaaacaaa aatttttcat tgcaataatt
    19501 gcaacaaaga aatttcatcg ggggaagagg tttggacaaa aatggaaatt tccgccaaaa
    19561 gtaactgctc ctcaactgaa atcaattaaa gaactagaat ttgagaatgc actggtaatt
    19621 tgtatgacat gtactgatga agtatttcga aaacattttt aaaggttgag atgtatatag
    19681 atttaaccaa tcatttatct ctctcggaaa aggaatatta actaaccagc gggttatcaa
    19741 aaataggtag gaaaactgta cttaatggct tatttcgcgc tgattatgag aatatcagtc
    19801 tgtttgatta atttttaggg ttcaaaatac agagacaaac tccaggtcgg gtttcgccac
    19861 aagcgaatta cccgatctgg agttttgtct attgcaggaa ggcgtagccg gactgcagga
    19921 ataaagcccc cggttctaac agggggctaa caaagaacag gaaactgtga caactaccca
    19981 ttggggagtg tcctagcagt attttctttg tcacgtgctt atcaattttg tcacctcatt
    20041 ttgatgatgg atacgctgca tttaacaggc gttctttctc gacttccatc acgaatggta
    20101 gataaagata ttgctgttct agtagatatt gattgtcctc tgtgtactga taagaagcgg
    20161 tttcttctga atagaccaac ggggcaattc gcacatcgct ggtcaattga ttaattgagt
    20221 atagaaggat ggctttctca cctagttcct tgggtaaatg tttcccaaaa taggaatagg
    20281 atttttgatt tgctaaatag acgagtatgc gatccacact aaagttggag aaggtaaagg
    20341 gcatcatgtg cagaatatga ttttgattat catgttgcaa atagagctgg gcttccttac
    20401 caaaattagt tgaaccaaga taaagctgct ttttttcatc gatacattgg aaagtaaact
    20461 ggtcttgttc agttggtgga atgagatttg taataaaaaa ttcaatcatt cgatccattt
    20521 ctttttcact aacaaggtca gcgtgtttga tttcgtctac tatgagacct tcaatcttag
    20581 gatcaagctt acctgccttt ttttggagca tttctaaaat gtattgattc tcttgatcac
    20641 catacaaaag atattcattg gtgacatgac ctatctctgc gatttggtga agggtttcgg
    20701 tttttggaag gttgatccca cgttcccagt tattgactgt acttcgcggt aaatcaccaa
    20761 ttagtttgcc aaatttctcc atactcaact ttaattcttg gcgaatctgc cgaattcgat
    20821 tgccgactgc ttgcttgtct atctccatga aaactcctcc tcaggataag ttcttaaatt
    20881 tagtatacgt gtgtacttaa aaaaagtcaa aacatattga cttaaaataa atacaggcgt
    20941 acaataaaaa tgtacttaaa ataagttcaa taggttcttt gacaagtaaa tagcagaagg
    21001 aggtttgtcg atgaaccaag ttaatcaaca tgatctcggt gaagccattc gagtttctcg
    21061 agaagaaagg gggtggacac aacgatattt agcagaaaaa gtggggattt cgagatagtt
    21121 attatcgaaa gtagaaaaag gaacgagaca attaagtgaa gaaaaattga atcttgtgtt
    21181 ggatagtttg caagaagagg tcgttccagt taaccgtgtt ttgattgatt atctaaccat
    21241 tcactttttc tcgaatcaac atttaaagct aattgaagag attattgaaa tgcccattga
    21301 acgttttgag gaactggatt atgcgccaaa gggctatatt ggccaatacg tgtggaatca
    21361 agtaatcacg attcggtatt ccattgacga tgcggttaaa ggaactgtga tggagttttc
    21421 aggtcaaggc tgtaaacact tagcaagatg gctaaaaaca cgaaaaacaa attggcaaca
    21481 gttttttcgt aaggtattag attatcaggg gaactttaca cggattgatt ttactttaga
    21541 tgattttgtg gggagtctca gcattccaga acttaagcat aaggtaatac taggccatgt
    21601 gtggaccact tttcaagtga gtgaatctca tggcggtacg gatatcataa acaatgaatc
    21661 aaatggtgag accttgtatt taggctcaaa gaagagtcag tgtcgttttt gcttttatca
    21721 gaaggaccat gaacaacgga agcgacgagg aattccttta gaagaggcaa aagtaaagaa
    21781 tcgctttgaa ctacggtatc gaaaagagaa agcgcaaagc ttggcaaaga ttatttcaaa
    21841 aacccatgat ttaaccaaac tgttctttga gttacttaat ggagcaattt gtttttatga
    21901 ccgtgatcca aatgatctag gtgcaaaagt tgatgccaaa tgggcagcct ttattagcaa
    21961 ttatggcgca atcaccattt ctttagatac aattcctcaa agttttgaga aaactatgag
    22021 ttggttaatt catggtgttt caccatcatt agtttttatt gcacaggtcg atcaaacgtt
    22081 tcattcttcc ttgttgcctt tgattattga acaaggagaa atgaatccaa gacagatcaa
    22141 acttctccaa aatatgaagg cagacccgga gtattatcag gaggaggttg agtattatga
    22201 acgaaaatta caaagtatat caaatgagaa aacaagctac taaaaaagca aagtactgct
    22261 tacacaacaa gttgtgcaac gtactccgcc tcgacaagac gagtataaca caaccatcga
    22321 atgagaaaca atagattata aagaggatta ttttaaaagg agtaaagaat caggaggtga
    22381 gagaatggaa acaaaatgtg atttcatggt aaatcgtgca attttgatag agatgggctt
    22441 taaacctagt caagctgcac gaatgattaa agagagcaag gcatatcttg caaggattga
    22501 aggtattgat ttttataata atcgccaagt tggtgtggtg ccatcccgag taattgaaca
    22561 tctttttcac attcaagttg cggaataaac agtacaagcg cggtactatt aaagtagctt
    22621 ttctcaagta cagggaggaa tggaacgatg ccaaaaatta aaaatattta ccaagataaa
    22681 aagacaaaaa aatggttctt ccgagcgtat ctaggtatag atgaaaatgg aagaaaggtt
    22741 cagaagacta aacgaggtta tgcttcacaa aaagatgcca aaattgctta tgacaagtac
    22801 atggaaacac atgattttaa caagacaatt cctaatcaac tgtgttctac caatcaaatg
    22861 actttcgaag agttttacaa ggataggttt gtaaaatggt acgagagaca ggtcaaaagt
    22921 caaacatatg agaatgcgca attcattttc gaaaagcgta tgcagttttt ctatcatttt
    22981 cgtgttcgag atattacaag tcacgatatt gaggattggt tatttgaact aagccagaca
    23041 gagtcacgta attcacggaa aaaagttaat ggcgaaacgt tatcaaaatc ttatatcaat
    23101 cggattcgag ggcacctaaa aattgtattg gatcgggctg ttaaagaggg ggtgattgct
    23161 aaaaatcccg ttgaagatgt ttcttcgctt ccttctgaga ataagaaagt tgagttttgg
    23221 gaacacagtg agtttcaaaa agtgatgaat gaattgaagg atgataagat tcaaacccac
    23281 catcggaaga ttgtgtatga aatgctcttt tatactggca ttcggattgg agaacttgaa
    23341 gctttgtctt ggtcgaatgt agattttgag aaaaacacga taacgattga gaagacattg
    23401 atttataata caaaagatga ttggtacttt tcgactccca aaactaaatg tgcttatcga
    23461 acgattgggt tggggaagaa gct
//
LOCUS       DAYLZO010000032        23297 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.032, whole genome shotgun sequence.
ACCESSION   DAYLZO010000032 DAYLZO010000000
VERSION     DAYLZO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.032"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..506)
                     /locus_tag="ACGVJG_001149"
     CDS             complement(<1..506)
                     /locus_tag="ACGVJG_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFJ6557121.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="ACGVJG_001150"
     CDS             779..3250
                     /locus_tag="ACGVJG_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFJ6557122.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="ACGVJG_001151"
     CDS             complement(3353..5089)
                     /locus_tag="ACGVJG_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6557123.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="ACGVJG_001152"
     CDS             complement(5086..5790)
                     /locus_tag="ACGVJG_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFJ6557124.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="ACGVJG_001153"
     CDS             5921..6424
                     /locus_tag="ACGVJG_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFJ6557125.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="ACGVJG_001154"
     CDS             6384..6722
                     /locus_tag="ACGVJG_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFJ6557126.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="ACGVJG_001155"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="ACGVJG_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFJ6557127.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="ACGVJG_001156"
     CDS             8273..8851
                     /locus_tag="ACGVJG_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFJ6557128.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="ACGVJG_001157"
     CDS             8855..9376
                     /locus_tag="ACGVJG_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFJ6557129.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="ACGVJG_001158"
     CDS             complement(9455..10009)
                     /locus_tag="ACGVJG_001158"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFJ6557130.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="ACGVJG_001159"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="ACGVJG_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFJ6557131.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="ACGVJG_001160"
     CDS             10549..10800
                     /locus_tag="ACGVJG_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFJ6557132.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="ACGVJG_001161"
     CDS             10925..11716
                     /locus_tag="ACGVJG_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557133.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="ACGVJG_001162"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="ACGVJG_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFJ6557134.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="ACGVJG_001163"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="ACGVJG_001163"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFJ6557135.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="ACGVJG_001164"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="ACGVJG_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFJ6557136.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13773..14909)
                     /locus_tag="ACGVJG_001165"
     CDS             complement(13773..14909)
                     /locus_tag="ACGVJG_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6557137.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPNLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKMPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPKGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15026..15718)
                     /locus_tag="ACGVJG_001166"
     CDS             complement(15026..15718)
                     /locus_tag="ACGVJG_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557138.1"
                     /translation="MKKVILGLVFGAFFLTGCSQSNVVDNTGKTNEKDSSVTLSESTT
                     QSSKNSQEVDGPLLKVGQWTKEKRDTSGKIELIGTASPQSDIPLGDIGIYISNIKLLK
                     YSNYADPSAAGYYDANNHKDSEGNFYGLQIAFKVKNNSEVDYGYNGLAHAILDNGQQI
                     DFSSDDLMHTMTTNSFFKKTESKEFYSLAYLDPSKVNDITKVTIKTGVLYNPSDYSTV
                     AESAESTFNINR"
     gene            complement(15856..16509)
                     /locus_tag="ACGVJG_001167"
     CDS             complement(15856..16509)
                     /locus_tag="ACGVJG_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557139.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKVSKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     GQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(16559..16987)
                     /locus_tag="ACGVJG_001168"
     CDS             complement(16559..16987)
                     /locus_tag="ACGVJG_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFJ6557140.1"
                     /translation="MEMDTINLVEELKRKYRSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIDELKNLYGLPESAYRFLI"
     gene            complement(16992..17366)
                     /locus_tag="ACGVJG_001169"
     CDS             complement(16992..17366)
                     /locus_tag="ACGVJG_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6557141.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            17651..17836
                     /locus_tag="ACGVJG_001170"
     CDS             17651..17836
                     /locus_tag="ACGVJG_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557142.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            17849..18097
                     /locus_tag="ACGVJG_001171"
     CDS             17849..18097
                     /locus_tag="ACGVJG_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557143.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKITPKEFIEKHLVDL"
     gene            18128..18307
                     /locus_tag="ACGVJG_001172"
     CDS             18128..18307
                     /locus_tag="ACGVJG_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557144.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(18350..18820)
                     /locus_tag="ACGVJG_001173"
     CDS             complement(18350..18820)
                     /locus_tag="ACGVJG_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557145.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            18907..19605
                     /locus_tag="ACGVJG_001174"
     CDS             18907..19605
                     /locus_tag="ACGVJG_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFJ6557146.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            19783..20124
                     /locus_tag="ACGVJG_001175"
     CDS             19783..20124
                     /locus_tag="ACGVJG_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557147.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            20117..20788
                     /locus_tag="ACGVJG_001176"
     CDS             20117..20788
                     /locus_tag="ACGVJG_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFJ6557148.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            20794..21480
                     /locus_tag="ACGVJG_001177"
     CDS             20794..21480
                     /locus_tag="ACGVJG_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFJ6557149.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVTNFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            21483..21710
                     /locus_tag="ACGVJG_001178"
     CDS             21483..21710
                     /locus_tag="ACGVJG_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557150.1"
                     /translation="MINLNAVAFEQTWRTKYKKMSPRDKLFLEIMTFAFIGTQAEQSD
                     ISVEKIKTNRLVNGITETCYQYTIIVVDEEE"
     gene            21713..22540
                     /locus_tag="ACGVJG_001179"
     CDS             21713..22540
                     /locus_tag="ACGVJG_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HFJ6557151.1"
                     /translation="MAERRMFAKTIIDSDAFLDMPLSTQALYFHLSMRADDDGFINNP
                     KKIQRMVGCGDDDLKLLMAKRFILVFESGVIVIKHWKIHNYIRNDRYKPTLYQDEKAL
                     LADKDNKAYTFAEELSKHDEKLGIPDDNQAVYQMDTQVRLGKDRLGKDSKEIKDLTPS
                     KKSKAKPIRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESSGKTYKNYL
                     ATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            22552..22821
                     /locus_tag="ACGVJG_001180"
     CDS             22552..22821
                     /locus_tag="ACGVJG_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557152.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            22826..22990
                     /locus_tag="ACGVJG_001181"
     CDS             22826..22990
                     /locus_tag="ACGVJG_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557153.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            22983..>23297
                     /locus_tag="ACGVJG_001182"
     CDS             22983..>23297
                     /locus_tag="ACGVJG_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557154.1"
                     /translation="MDKKAAMKRIIELTHSESWQEDEEVVAEVQRLGKIANGRIRKKP
                     DKRKQRKRKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYKYL
                     EEE"
BASE COUNT         7705 a         4055 c         4646 g         6891 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa
    13681 gggttctttt tttattatct gtttttttat attaacgatt tctatttggt acatttttga
    13741 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13801 ttttctttta ctccttttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13861 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    13921 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    13981 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14041 agaaattgac ccttcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14101 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14161 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14221 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggtctcat tccagtcaaa
    14281 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atctactagt
    14341 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14401 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14461 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
    14521 tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat accagaaacg
    14581 atgtaagtct caggcatctt cttctttata gtgtttaaga tattattcgt aggataatat
    14641 gtagactcct taacttgcct tttgtaaata actaaccatt cttcgacaag ctcatgaaat
    14701 gttagattag gcttctggag tgttttttca tttagtttta tatcaatttt tttatttaac
    14761 tccagtaatg ccactttctg agtttctcga gacttatttt tataagtaat gctcactttt
    14821 tttctttttc tagtttttgg atcaatatat cgttcattat atttatatac tttttttccg
    14881 tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt gctataatag
    14941 gaatggataa gtaagccgat tatagcaggt ttatttttca tcacgtccac aaactttggc
    15001 gagggagggg gcgtgttttt gtattttaac gatttatatt gaatgttgat tcagcacttt
    15061 cagcaactgt actataatca gacggattat ataacactcc ggtttttatc gttacttttg
    15121 taatatcgtt aactttagat ggatctaagt aagctaaact ataaaattct ttactttcag
    15181 tttttttgaa aaacgagttt gttgtcattg tatgcataag atcgtctgaa ctgaaatcaa
    15241 tttgttggcc gttatcaagt atggcgtgtg caagcccatt ataaccataa tctacttcag
    15301 aattgttttt aactttgaat gcaatttgta aaccataaaa atttccttca ctatctttgt
    15361 gattatttgc atcgtagtaa ccagcagcgc tagggtcagc ataattggaa tacttgagta
    15421 gtttgatatt agatatataa atgccgatat caccaagagg aatatctgat tgtgggcttg
    15481 ccgttccaat taattcaatt ttgccacttg tatctctttt ttctttggtc cattgaccaa
    15541 ctttgaggag ggggccatca acctcctgtg aatttttaga agactgtgtt gtactttctg
    15601 ataacgtaac tgaagagtct ttttcgtttg ttttgccagt attatctaca acattagatt
    15661 ggctacatcc ggtaagaaaa aaagcaccaa atactaaccc taaaatcact tttttcattc
    15721 tttattctcc gtttctatga tattattttt atgtaggatc ttagaaacga gatcttagtc
    15781 cgtgttgcag cacgggcttt tttctttata acttttttag agattatagg caaaatagta
    15841 ggacacaaaa atatattatt gtattccgta tttagaaaat cctaattgaa cttcaccaga
    15901 agttttttgc tgtgttgtac gcaatgcttc ttcttcagac attccattct gtactttcca
    15961 tgcaacaggc gacatcccgt atttgttaac aaaatcagta agtgataaag tgtcagcgtc
    16021 ttgctgaccg cttgtttgtt ggtcttctgg attttgttga gatgctgctt gttgttcttt
    16081 ctgatcttga ctgataatat tgccagcatc atctgtagtc aatccatttt cataaagggc
    16141 cacgccgaaa gcttcccact ctttgttgga ccaatttgca cgatcagctg gagttgactg
    16201 taaagtgcgt tgtttcatct gttcatatgt ttcttcttga ggtgcggttt ggattgtatc
    16261 ctgactggag cttataactg ttgggctagg ttccgccgta gcttggttag agcttgtaac
    16321 tgttgaacta gaatctgtct tagatgtaga tttgctagta gaggaactgg tttcagttgt
    16381 ttcttttgtt ttacttactt ttgtttcttg gttagaagtg gcatctgttg attcagcttt
    16441 tttattattt gaacaagctg aaagtagcag agcagtactt aacaacaaca taacgctaac
    16501 ttttttcatt ttataattcc tctttctcgt tgtaatatgt gtgctaacac gggctttttt
    16561 atataagaaa acgataagcg ctttctggaa gtccgtaaag attctttaat tcatcgattt
    16621 ttttgggata ttgatcatta tcttctttat aaagagaaac aatgagatta gcagcaaagc
    16681 aattagcttc gctttcagat ttgcttctag atgttcttgt tgatacatag taactggata
    16741 agccgcgatg aaaaatagcg tgacctaatt cgtgagcgca aatgtagaat ctttcctcag
    16801 agtctcgcag ttcatcattt aagaagatta ttgcacgacc tctaatttct tgaaactgtc
    16861 ctttgggatt ttcgataaaa ggaacgtatt gaattttaat gcccatcttt tcacaaatat
    16921 aaaaaggatt agcagaccgg tatttccgct tcaactcctc gactaaatta atcgtatcca
    16981 tctccataag ctcacatctt tttgcctttt tctttgtctt ctttcacaat atcccagaag
    17041 gttccaataa ggatatcttt tacgcgttgg acctgttcag gcgtcagtgt ttctccgcca
    17101 taagacatat tgacatttga atctagtaat ttatcaagtt caactacttc atcttttgtt
    17161 gcccagtcag gaacttggtt tctaccgagt aaataatcag tagttacatt aaaataatct
    17221 gctacaagtt gtagtctttt agtactagga gttctatttt tccattgata aatcgtgttt
    17281 ttaggtatat ttaattcttc ttccaattga gttatactta tgcctctttt atgagctaat
    17341 tcctttattc tatctagtaa attcattttc gcacctcaca agctacgaaa acactaataa
    17401 aaaaattagt taaaggtgtt gacaactaat aaaattgtta gtatactgtt ttcgtaagct
    17461 aaaatattag ctaaaaagac ttgataacta ataaacactt cacggtcggc aaactctgaa
    17521 atgtaaatta ctaggcattt ctgtgtctta tttagctata tctatatact aataaaaata
    17581 ttagttattg tcaacgaata ttagctaatt ttttagctta cagactattt tttagaaagg
    17641 agctattttt atgtctgaga atttagactt aaaaattcga gcggagatga gaaaaagaag
    17701 aatgactttc aaagaactag ctgcgcttgt tggtatttca ggagcttatt tatcagatat
    17761 tctaaacggc aatcgtgatg gaaagaaagc acaacagcat atcgaaacag tgaaaaaaat
    17821 attggacatc cgataggggg ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa
    17881 aaaatacaat tattctccat ctacttatca acgtcgaatg tcggaactaa aaaatacagc
    17941 aattttctca gcggcgtatg aacgggtcac aggacaagaa gtttggatca atacagaatt
    18001 atacgataaa tttttgtctt tcaaatccta taacaggtta cgcacaagaa agataacacc
    18061 taaagaattt atcgagaagc atttagttga tttataaaaa acagaacatt ttgagaggtg
    18121 aaggttaatg ggcaaattca acagagcgct agtgttcagc gcaccgctaa tcatctacgc
    18181 tttaggcctc tggggaagca ggcaagcgtt gataggaacg attgtttaca tggtttggat
    18241 ttttatgggg cttgatgaag ctgaagctga gtacagagcg aaaaagccaa ccgagggggc
    18301 tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact cagtttaatt
    18361 ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt ttgtggattt
    18421 gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta ttaaaattaa
    18481 ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga atatttagcg
    18541 gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca attaatttct
    18601 taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct aaattaacta
    18661 acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc atatctgcag
    18721 cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt atttttatcc
    18781 ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa attgttaagt
    18841 gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa aaggagagaa
    18901 gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac gagcttacaa
    18961 gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga tgcactaaaa
    19021 aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga ttcatatggc
    19081 agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc tatggggttt
    19141 acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa cgaaatggaa
    19201 gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat ctatcaatta
    19261 gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat gcgaattagc
    19321 ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg agttctaggg
    19381 ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa attttggtct
    19441 gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa gagattcgat
    19501 gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat ggaaattgat
    19561 caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg aagcgctaag
    19621 aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca tcctaagcca
    19681 acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc gccggcaagc
    19741 aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa agaactttcc
    19801 actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga ggaaacaagt
    19861 ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga tcaagatgat
    19921 ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc gaagcattcg
    19981 aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct agaagagtac
    20041 gacagagata acttgattga tatctcttat tacggaccag gtaagaaata cattgctgaa
    20101 atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa aactttttag
    20161 tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat cttgggctca
    20221 cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta atgagtacaa
    20281 acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat ataaaccttt
    20341 tcttatggat gaaactggga cttatgtatc tgtctcagta acggttaaag gacacacgga
    20401 aaccgaatta tttcctgttt tagattatcg aaaccaacca gttgttaaac caaatgcaat
    20461 gcaaatcaat aactcattga agcgatgctt tgtgaaagca ttggctctac acggactggg
    20521 attatatgta tttcaagggg aagatattcc aacaccacct agaatcgata caaagaaatt
    20581 aaacatgcta gagacgattc tagaagcttt caatgagcag atgggtaaag atatgaccaa
    20641 aaccttaatc gaatatgtta atgagcagac agataagtta gggctcttag ctgataacgt
    20701 tgaaactatt gaacagttaa gctatgagca atgtgccttg atggagcgag caatagcagc
    20761 taagagaaaa gaattagata agaagtgata tgagtgttta aaccattaat cgattcatat
    20821 tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa tgaggaagtg
    20881 aacattgatc gactaaagac gatgtatgac ggctacgatg gtgatcgagt tattgaaatt
    20941 cgttttattg atcctagacg tttcacagta cagcaacgaa acttcatcta tgcactcata
    21001 ggcgatattt tcatcgatac aggcatgcca acggacttct ggaaggaatt cttttacttc
    21061 cgtttcgaag gtgtcacagg gcgcgaaata agcctcaaag atgaatcgaa tacgactgtg
    21121 agtgatgcca atatcttagc aaatatcatc ttagatttta tctttgaaca tcatattcct
    21181 ttcaaagaag gctatgagat tttaccagcg aatcaagaat attacttcta caaatgcatc
    21241 acaaaaagag tctgctgtat ctgtggcaaa acaggagctg atatcgatca ctttgacaaa
    21301 gcgctaggaa gacgaaagcg caaagaagtt gatcattcag agtacacatt tgcagcactc
    21361 tgtagaattc atcacacgga gaaacacaaa ataggtgtga ccaatttcaa aaataagtat
    21421 caaatcaaag ggattaagtt aaaccaggag acaatcaaga aattaagaat cgggggctaa
    21481 tgatgattaa tttaaacgca gtagcgtttg aacaaacatg gcgcactaag tataaaaaaa
    21541 tgagtccaag agacaaattg ttcttagaaa tcatgacatt tgctttcatt ggtacacaag
    21601 ctgaacaaag cgatattagt gttgaaaaaa ttaagacgaa tagattagtt aatggaatta
    21661 cagagacttg ttaccagtac acgattatcg tcgtggatga ggaggaataa ttttggcaga
    21721 aagaagaatg ttcgcaaaga caattattga ttctgatgca ttcctagata tgcctttatc
    21781 gacacaggca ttatatttcc atctatcaat gcgcgcagat gatgatggat ttattaataa
    21841 tcccaaaaaa atacaacgta tggttggttg tggtgatgat gatttaaaac tgcttatggc
    21901 aaaaagattc atcttagttt tcgagagcgg tgtgatcgtc attaagcatt ggaaaataca
    21961 caactatatt cgtaatgatc gttacaaacc cacgttatat caggatgaaa aagctttact
    22021 agcagacaaa gataacaaag cttatacatt tgcagaagag ctttctaaac atgatgaaaa
    22081 acttggtata ccagatgaca accaagcggt ataccaaatg gatacacagg ttaggttagg
    22141 taaggatagg ttaggtaagg atagtaaaga gataaaagat ttaacgcctt cgaaaaaatc
    22201 gaaggctaag cccatccgtc ataaatacgg agagtataaa aatgttcttt tgtcagatga
    22261 gcagatggag aaactcaaaa cagaattccc taatgattac caagagcgaa tcgaacgact
    22321 gtcagagtat tgtgaatcat ctggtaagac ttataaaaac tatttggcaa ctattcgaag
    22381 ttgggcaagg aaagaaaaaa gtgaacctaa gaacgcaagc agtggataca agcgcacagg
    22441 aagacgagag aagcttcctg aatgggcaat cgaccaagaa gcctatctca agaaaaaagc
    22501 gctagaacga gctaatagac aatcaaaagc accattttaa gaggtggaaa attgaaaatc
    22561 gattatctag aactaattaa tgaaatagca aagtataaaa ctggtgagga aatagaaatc
    22621 ctgagagacg tatatgatca actcgaagaa gctggaatcg aaggaattaa taatgatcgt
    22681 tcgagttgga gtaaactcag atactatttc gcactctata tcgatacaac acaattaaga
    22741 aatttagctt atacaaaatt actatttgtt gattgcgtta aaggattgca aaaacatctt
    22801 agtgaacttg agcaggtgta atcagatgga tctaaaaaca tttacagcac agatcgaact
    22861 aatgcatcaa gaagctttaa gacaaagcgc ctcgtacgaa gacaagtggc tcaacacgtt
    22921 ccatggtgga cgtgagagcg cacttgatca agtactcaaa ttattgaaag gggaacgtcg
    22981 ggatggataa gaaagcggca atgaaacgaa tcattgaact gacgcattct gaaagttggc
    23041 aggaagatga agaagtggtt gctgaagttc agcgactagg aaagatcgct aatggacgta
    23101 tacgaaaaaa gccagacaaa agaaaacagc gcaaaaggaa aatcgtcgtt gttttacacg
    23161 aaggcaaaat tttgatgcaa ggaacagcta gtgagctgtc tgcagaaact ggatatacgc
    23221 gtgggactat tcgaacgtac gcttggcgaa atcacaccga tcgaaaaggg cacgaatata
    23281 agtatttgga ggaagaa
//
LOCUS       DAYLZO010000033        23193 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.033, whole genome shotgun sequence.
ACCESSION   DAYLZO010000033 DAYLZO010000000
VERSION     DAYLZO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.033"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..416)
                     /locus_tag="ACGVJG_001183"
     CDS             complement(<1..416)
                     /locus_tag="ACGVJG_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFJ6557155.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKR"
     gene            646..1548
                     /locus_tag="ACGVJG_001184"
     CDS             646..1548
                     /locus_tag="ACGVJG_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFJ6557156.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(1589..3475)
                     /locus_tag="ACGVJG_001185"
     CDS             complement(1589..3475)
                     /locus_tag="ACGVJG_001185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFJ6557157.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(3572..4423)
                     /gene="licT"
                     /locus_tag="ACGVJG_001186"
     CDS             complement(3572..4423)
                     /gene="licT"
                     /locus_tag="ACGVJG_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFJ6557158.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(4881..6665)
                     /locus_tag="ACGVJG_001187"
     CDS             complement(4881..6665)
                     /locus_tag="ACGVJG_001187"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557159.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(6665..8416)
                     /locus_tag="ACGVJG_001188"
     CDS             complement(6665..8416)
                     /locus_tag="ACGVJG_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFJ6557160.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(8558..8782)
                     /locus_tag="ACGVJG_001189"
     CDS             complement(8558..8782)
                     /locus_tag="ACGVJG_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFJ6557161.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            9033..10619
                     /locus_tag="ACGVJG_001190"
     CDS             9033..10619
                     /locus_tag="ACGVJG_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFJ6557162.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            complement(11066..12283)
                     /locus_tag="ACGVJG_001191"
     CDS             complement(11066..12283)
                     /locus_tag="ACGVJG_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFJ6557163.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(12540..14315)
                     /locus_tag="ACGVJG_001192"
     CDS             complement(12540..14315)
                     /locus_tag="ACGVJG_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFJ6557164.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(14528..15430)
                     /locus_tag="ACGVJG_001193"
     CDS             complement(14528..15430)
                     /locus_tag="ACGVJG_001193"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFJ6557165.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            15709..18054
                     /locus_tag="ACGVJG_001194"
     CDS             15709..18054
                     /locus_tag="ACGVJG_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFJ6557166.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(18390..19391)
                     /gene="ccpA"
                     /locus_tag="ACGVJG_001195"
     CDS             complement(18390..19391)
                     /gene="ccpA"
                     /locus_tag="ACGVJG_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFJ6557167.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            19601..20707
                     /locus_tag="ACGVJG_001196"
     CDS             19601..20707
                     /locus_tag="ACGVJG_001196"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFJ6557168.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILSGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            complement(20775..21377)
                     /locus_tag="ACGVJG_001197"
     CDS             complement(20775..21377)
                     /locus_tag="ACGVJG_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFJ6557169.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(21380..21820)
                     /locus_tag="ACGVJG_001198"
     CDS             complement(21380..21820)
                     /locus_tag="ACGVJG_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFJ6557170.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(21945..22883)
                     /gene="galU"
                     /locus_tag="ACGVJG_001199"
     CDS             complement(21945..22883)
                     /gene="galU"
                     /locus_tag="ACGVJG_001199"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFJ6557171.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(22898..>23193)
                     /locus_tag="ACGVJG_001200"
     CDS             complement(22898..>23193)
                     /locus_tag="ACGVJG_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFJ6557172.1"
                     /translation="GDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLANMGMVVEGVNTT
                     QAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKENEFSLTIF"
BASE COUNT         6780 a         4791 c         4245 g         7377 t
ORIGIN      
        1 aaacgcttca tgtcttcaac gattgccaaa accactggaa cggtacgaaa agctttgaat
       61 atcccgccag caccatttgt ttcttgccca atcatcccat aataactagg gatccgctcg
      121 tctttgattc gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa atccgcatct
      181 ttcaatgctt cttcccgatc cagagttgca tagattttga cttcatacgg agaagcatcc
      241 cacatacgct gagacatctg actgacgatc tcaagctttt cttttccttc ttctacatct
      301 acgagccaga tttctcgtat cggtaattcc tcatatcttt tgataaatcc ttccatcaac
      361 tcgggggtgt aactgctgcc tccaccaatc gtagcgattt tgattccttt tgccatgttc
      421 tcactccttt actttttaca cttttattat cttttttgta aacaaaacaa acaatagaaa
      481 ttcgtttctt tccagtaaca tttacaaaag ttagtgtttt ctactggatt tttgtaaata
      541 tcttgtcaaa aaatgtcttt attaagctat ataacataaa tccgtttaca aatcattttc
      601 aaaaaagtta taatttacgt aaaaaaacaa gaagaaggta tctacatgtt attacgtgaa
      661 aaaatcaaag aaatctcctt ttctccttca gaaaatgaag tgattcagtt tctcttaagc
      721 gattctccag cacttggcga aatgaccatc caagagattg ctgagcgatg ttatgtccac
      781 ccgtccacac tcatccgtgt agcaaaaaaa atgggttttg atggctgggt cagttttcgt
      841 aatgcttttt tagctgaaac agcctatctt gaagggaatt ttcaagacgt agatgcgaat
      901 cttccttttt ccaccacaga cggtattatg acgatcgcaa ataaaatggc ttctttagaa
      961 cagatgacga ttcaagatac actttctctc attcatcatg accaactgca aaaagccaaa
     1021 caactaatgc ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa tactttgatt
     1081 tctcaagatt tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat tgtcaccact
     1141 tttggtgaat cgttctatga agcgtacaat tgcacgagtg atatgtgcgg gattctgatt
     1201 tcttatacgg gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa acaaaatcat
     1261 gttccaattt tagctttgac cagtatcggt gattcaacgc tatcttcaat gagtacagcc
     1321 gctttgcgac ttacgacacg cgaacgactg tattcgaaaa ttggtaattt tacgatcaat
     1381 acatcgattt gttatcttct agacgtgtta tatagttgtg tttttgcaga aaattatcaa
     1441 cagaatttag tccatctaat ttctgtagga caaaaatttg ataaacgccc tatcagctca
     1501 gccatcatga aagagccttt caattttgaa aataaagaag tggactaaaa agaataatta
     1561 agcctagtcc attctttcac ctaaccgttc aggtcagtgc ggtgataagt gcatcgtctg
     1621 gcccgaccgt ctccccttga ttttccaaaa tatccaaata atccgtgtaa ttggtgacga
     1681 tcacaggtgt ttctaaactg tatccttcct cttcgatttt ctttttgtcg aagcggagca
     1741 acagatcgcc ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa tatttccctt
     1801 ccaactgaac agtatccaat ccgacatgaa tcagcaattc cagacctgct tcagaaacca
     1861 ttccgattgc atgtttcgtc gggaacaggg tcatgactgt tccgtcaaaa ggggcacgta
     1921 cttctccttt ttcagggtag atcaagattc ctcgacctag tgttccttgt gcaaaagctg
     1981 catcctttgc tgcattcagt ggagcgattt gtccttgtat cggtgttttt acaatttctt
     2041 tacctgatct tttctgtcta tcatctgttt cacctgcttc ctccttccaa aagaaaaaag
     2101 tcagcgcaaa ggataccacc atcgcaatgg cgattccaat caatacatta atcacatccg
     2161 ttaaatcacc atctggtcta atatagttgg gaattgtaaa gatgcctaat ccacctggat
     2221 tccaagaagt taaattcatt aatccactat acgcgccccc taacgcacca ccgacacagg
     2281 taaaaataaa tattttttta cgtggcaatg taaacccata aatagctggc tctgtaatcc
     2341 ccacaatacc agaaacaatg gctggtattg cgattgcttt gtcttttggg tttctaagct
     2401 taaagtagag tgcagtaagt gctgcagttt gtccaaaaga cactactgca acaggtgtca
     2461 tgatattttg ccaaccattc acagaaaatt gcatcaatcc cattggtacg attgcccaat
     2521 ggagaccaaa catcactaga atttgccaga agaagcctac gattgctcca taaataactg
     2581 gcgaaagtgc taaaagtgtt tcaaatccag ccattaaaat atcagttgcg aatgtgaatg
     2641 ctggtccaac gagtaaaaaa cctaagggca ttgtaataag aacggtgaag aatggaacga
     2701 agaaattcgc gatcatctca ggtactaact ttttaattgg tttctgaatt tggctagcta
     2761 acaacacaat aaagataatg ggaatgacag aagaagcgta gttacgcgca atccagggaa
     2821 tgccgaatac tgtttgataa ataggtgctt caaaaatagt gccagaaaat atggtagtca
     2881 gtggtgcttc tgctgcacca ctcaaagctt ccagcgacaa gctaggattc attaatgctg
     2941 ccccaagcaa catccctacg aattcattta ctttaaattt acgggcagct gttaaaccga
     3001 taataacagg cataaattgg aatacagaat caccgatatt atcaaacata acaaatgtgc
     3061 cagaaccgcg gtcaaatgcg cctaagaaga caagtaatgc tgtcagtcca cgaatcatcc
     3121 caccagcagc tagaatagca aggaatggct ggaaacagcc cgatattcca tcaataagca
     3181 tatcaaacgg tctcattttt tctgttgaaa cagcctcatc aaaagtaagg tttcctgctt
     3241 ttataacctc ttcaaacacg actggcacat gattcccgat gacaacttgg tactgtcctc
     3301 ccgctcgcat taccgtcaca acaccgtcca tatttttcaa gatctcatca tttgcttttt
     3361 cttcgttttt taatttgaaa cgaagccttg tgatacaatg tgtcaaacca ttgatatttt
     3421 ccttcccgcc gacattttct acaattttgc tcgctaattc ttgatatttt cccatttcta
     3481 ttccctccaa tttattagtg gaaggtttag ccaactgaat gtcactaccc ttatttcatg
     3541 cgttcttgtt aatttatcct ccttttttta tttatttaca acgattctca tgcactaaac
     3601 gcgcaatatg gatcgttaaa tacatttttt cgtccgtgga catctggtag ttgaatatct
     3661 cggctaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat tttttttgaa
     3721 taacctggta caattcctca gaaacttctc ccacttctga tttttctcca tctaggacgc
     3781 gtgtcagaaa aaaacgtagg tgtgtgacga atcgaaaaaa atggactgaa tcttcatcaa
     3841 atgtaatctt atagtaatag cggacgatat ttagaatatc ctgtattgtt gtagtaaaca
     3901 ggtaggtatt gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc aatgcgataa
     3961 atccggcttc atcttctgat aacttgatgt ggtatttatc cgagattttc ttcactgttt
     4021 ttttcccgat ggcatactct ttaggaaata gttgtttgat atcatacagc aatacatttg
     4081 gtacaaatat tcccattttg tgacgttcga tagccgtata caaatgatcc gtcaatgcaa
     4141 tatacaccga atcattcaac tggacttgta gagcctcttt tgtatattca atgatttcac
     4201 aagaaatttc taagtactca agaggaatcg cttgtgccaa ttcttggaac tgttgtgaaa
     4261 gtccgtcatt tgtcaactga aattttttgt cgatgcatga gtcaaaaatc gggtcaccca
     4321 ctttcttttt gaatgccaaa ccttttccca tcacgatcat ttcctgattt ctttcatccc
     4381 tggttattac gacgttgtta ttcagtactt tctcaatcag catgttttac tcctttccat
     4441 gatatatagg gctgtatcca aaacaaaaag aaaaacccaa aaagccgtcc atggaaccat
     4501 gggagactca tcaggtttag cttgcatcag gcaatcacta tccaagttgt atcgttttct
     4561 ttatattatc gaaagcaagc acttttgtca acgctttctt agcggctatt gggcacttct
     4621 gactagctgt cgtgattttt tttaaatctg gtaattacga cactaacgct cacagatgaa
     4681 gaatctggga agctctgttt tattttcact ctagcgctta ggtcaaaatg ctactatgct
     4741 actgtcccaa cttctaatat acggtgttcc agtaccagct tatcggtgat aagccaaatt
     4801 tatgcaaaaa tctggaaagc cttttttaca atttttttct tatcacctaa agctgaacgg
     4861 agccctgtca caaccacttt ttacacacca ctgctttgca gtttgtacat atctgcatac
     4921 aaaccatttt gcgctagaag tgtttcatga ttcccacgtt ctactatatg acctttgtct
     4981 aaaactaaaa tcaattctgc atcccgaatc gtggacagcc gatgggcaat cgcaatcgtt
     5041 gtccgtcctt gtcgcatttt tgtcaatcct tcttggatca atccttctgt ttcagtatcg
     5101 atatttgctg tcgcttcatc caatactagt attttaggat ctgtcacgat tgttcgcgca
     5161 aaagtgatca gctgtcgttg tccgctagaa tagctggcac cgtgttcgat cacttttgag
     5221 tgatatcctt ttggtaattc atcgatgaac ttgtttgctt gaacaaactc agctgcatgg
     5281 cgaatctgtt cgtctgtgat agttggattc atcatccgga tatttcctgc gatatctcca
     5341 taaaacaaga aggcatcttg caagaccaat cccattttct tacgtaactc ttccattgga
     5401 tattctcgaa tgtcatgatc atcaatcaag atctggcctt cataaaattc gtaaaaccgc
     5461 atcaatacgt tgatgatcga gctttttccg cttccggtgt gccccactaa tgcaacggtc
     5521 tctcccggat tagcgacaaa agagatattg ttcaatacat tgtgctctcc atcataagaa
     5581 aagcttacat tgcgaaactc gatcttacct gaagtaatcg cttgatctgc tccaggattt
     5641 tgttgcggtg catattcttc tgtatccatg attttcaaaa tacgactacc ggcaaccatt
     5701 ccatctgtaa aggtgctcaa aaagtccatc atttgagaca ttggattaaa aaatccctgg
     5761 acataggtcg taaatgcata gatcagtcca acttctacgg gagaatgcag cgcatcgatc
     5821 ccaaacaaag tcaacgccaa cgcaatcgcc aatgcatata gaaaattgat gatcggactt
     5881 aacagaatcg aatttgtttt gatcatcgca tagcgcgtgt ctaaatactc gttgttcact
     5941 tgttcaaatt ctttagctaa acgagcttct tggcgaaact gctggataat acgcatgccg
     6001 gaaatcgatt cattcaattt cgtgttcagc tgacttaatt tttcccgcat attccggtaa
     6061 atacgagaac tgaatttctg gtaataccag atgacgattc ctaaaatcgg caagaacacc
     6121 aaagtccaca aggcaatttc tccattgatt tgaaacatgg caatgaagga agaaacgact
     6181 gcaaagatcc cagtcaatac catcaaaaag acataccaaa attcaaatag cgtttctgtg
     6241 tcatttgtca ctcgagaaac agtcgatcct gccggtgtct gatcaaaata acgcatacct
     6301 agtgtatgca gcttttcaaa taatcttacc cgaacatact gataggtctt caatgaagcc
     6361 atggaataca aataccattg gaaaaaccaa atgatacttt tgactatgac tccaaacaag
     6421 tacataccag caaaaaacaa aataacttgt gtcgtagccg actgtttagc cagatgatca
     6481 tccataaaag tttggatgat ccttggcaat aggacattga tcacagacag ggcaaacgca
     6541 aataagatag caacgatgaa cgttcttcta aaaggtttgg cgaacttcat cagtcgtttg
     6601 atgatcttct tctgttcttt aagtggaatc gattttgtcc attccgattg ataattttca
     6661 tccatcaggc ctcgcctcct tctatcttcg cttctaattt ctgtttctcc cacattcgtt
     6721 cataccagcc gcctaatgca agcagctcat gatgggttcc tcgttcaatg attttccctt
     6781 gatcgacaac aatgatttct tttgcatgca tcacgctgct taaacggtga gctgcaatga
     6841 tggttgtttt ctcttttctt atttctttta aattatgcaa gatagcttct tctgttttgg
     6901 catctactgc agaaagtgca tcatctaaga tcagcaattc aggatttttg atcaatgctc
     6961 gagcaatcga aatcctttgt ttctgtccgc cagataaaga gactccacgt tctccaacta
     7021 aggtatcata accttttggt aattccttga tttcattgtt gataaatgct aaagaagccg
     7081 catgctctac ttcttcttct gaaagttttg gattagaaaa acggatatta tctcgaatgg
     7141 tcatcgaaaa caagaagtga tcttgaggca catagccgat tgatcctaat aaagcattca
     7201 atgaatattc tttgatcgaa catccgccaa attgaatttt tccttgataa tcatcgtatt
     7261 cacgcatcag taatttgatg attgtcgttt ttcccgcacc agttttccca acaataccta
     7321 atgtttctcc ttcttggatc gcaaatttga tttgttctaa tgctggtgat tcttctttcg
     7381 gatagttaaa agaggaaata gctacattca gctcgccttt tgccggagtc tgcactgcac
     7441 ccagttgttc cacgatatgc gtcttttcat gcaaaagttc attgacgcga tcataagaag
     7501 catttcctct ttccaatacg ttgaacaatc gaccaatcgc aaacatcggc caaacaagca
     7561 taccgatata gctaataaat gagaccatct gcccgattga gatcgtgcct tccaagacat
     7621 actttccacc taaaataatt gttgcaacat aagacagacc gataatcaag gtaatgaatg
     7681 gatcaaataa ggagtctaaa aaattgactc gtttgttttt aataattgca tcttcaatct
     7741 tttcttcaaa atccgcgaga tcttcttttt cttgtccaaa tgttttaatg actttgatcc
     7801 cagtaatact ttcttgtgtc ttatcattga tactagaaaa agcttcttgc gaatcacgaa
     7861 aagcatcgtg gagttttgac cccagcactc gtgaagtaac ggctagtaaa ggcagaggaa
     7921 tcagcgcaat cagtgttaag cgccaatcaa taaacaaaat catcgcaatg atcgttgttc
     7981 cgcccgtgat cactgaatct gcaaaggtca agataccagc tcctgctaca ttttggattg
     8041 cattcaaatc attcgttgca tgagccatca aatctccagt ccgatatttt tggtaaaaga
     8101 gattgtccat ttctgtaaaa tgtttgaata gctgctgccg aagttctttc tccaaccgag
     8161 ctgcacttcc ccaaatattc gtccgccaga tataacgaaa aagatattgt aagagagctg
     8221 caaggatcaa taataagatc caccaggaaa tgatctttat tgataaattt ttatcggcga
     8281 tctcatcaat gatgatccca atcacttttg gaggagctaa ctgtaccaag gccactgcga
     8341 atagggagaa aatcccaatt aaataatgtt tcttttcctg tcgaaaaaac caacctaatt
     8401 ttctaaaaat cgacatttta ctcctccttt gtctttatca agagcatagg attgtctgtg
     8461 cttgtctttt ttgctgaata gtctgaatag tctaacaaat tacgaaataa aaaacggact
     8521 gaaaaaatca gcccgcaaaa agtctttggg tcagaaccta ttttttccct tggttcttca
     8581 tttgctgcat catctggcga attttcttct cagatggttt ttgccccatt gacatcatca
     8641 ttgatcgcaa catgtcctca ttaacgggag gatttttttc taaatagtct ttcatatatt
     8701 tacgcgctag gaaaaagcca cctactgcgc cgattagcaa agcaataata gcaataagta
     8761 cgacaatacc tgttgatacc atataaatct ctcctttcta tttccgaatt gctctcaatg
     8821 atttatttta ctaaagttta ttgaaaaaag aaaggcgtat tctttagttt catgcaaaaa
     8881 atagtggttt ttttagagaa gaacaaaatt tctttcatta accagcaaga cacgccacga
     8941 aaaaaaatta ggcataccaa atctttttct ttaatttttt ctttctgagg tgtagaatgt
     9001 aatcgtagat aaataggaaa ggtggttttt tcttgaataa tcaatccaac aaacaatttc
     9061 ataagcatcg acttattgaa catacaaatg gtttatcttt agctgaagta aatggaacag
     9121 tggatgtacc caagggaaaa agttttttta aaacacttct tgcttactct ggaccaggtg
     9181 cattagtagc agttggttat atggatccag gtaactggtc cacttcgatc actggtggac
     9241 aaaatttcca atatttattg atgtcagtta ttttgatgtc tagtctgatt gctatgcttt
     9301 tacaatatat ggcagcaaaa ttaggaatcg tgacccaaat ggatttggcc caagcaatcc
     9361 gagcacggac aagtaagaca ttgggtgtca ttttatggat cttgactgaa ttagccatta
     9421 tggcaacaga catcgcagaa gtcattggtg cagcaatcgc cctctacctg ctttttgata
     9481 ttccattggt aattgccgta tttatcactg tgttagatgt tttcgtcctt ttattgctga
     9541 caaaagtcgg gtttcgtaaa atcgaagctt tggttgtctg cttgattttc gtcattttat
     9601 tcgttttcgt ctatcaagtt ggcttgtcta accctgattg gcacagtttg tttgccggat
     9661 ttatccctaa cagtcacaca ttctcagact caccggtcat cggcggcgaa actccgctga
     9721 ctggagcttt agggattatt ggtgcaacag tcatgccgca taacttatat cttcattcag
     9781 cagtttctca aactcggaag atcgatcacc aagacgaaga agatgtagca caagcggtac
     9841 gcttttctac ttgggattct aatatccagt tgtcaatggc ttttctagtc aatgcactgc
     9901 tattgatcat gggtgtggct gtattcaaaa ccggttctgt ccaagatcct tccttttttg
     9961 gtctgtatga agctttatct gatccatcag ctatgagcaa tggtatcttg attgctgtag
    10021 caaaatctgg tatcttgtct actttatttg ctgtagcact tcttgcttct ggccagaact
    10081 ctactattac tggtacgttg accggtcaag tcatcatgga aggcttcatc catatgcgta
    10141 tcccaatttg gctaagaaga ctagtcacac gattgctttc tgtgattcct gtattgattt
    10201 gtgttttgtt tacaagtaca aaaggaacga tcgaagaaca taccgctttg aataacttga
    10261 tgaatgaatc ccaagttttt ctggcttttg cattgccatt ctcgatgatt cctttactga
    10321 tgatgaccaa tagccaagcg gagatgggga gccgattcaa aaattcattt gttgtaaaac
    10381 tacttggctg gttctctgtg attagtctga cttacttgaa tttacgagga ttaccaggtc
    10441 aaatcgaaag tttcttcggc gagcaagcca cctcagctca actagcattg gctgatcaaa
    10501 tcgcttatgt aatcattgtt cttgtccttc tcctgttggc atggacgatt ggtgatttat
    10561 ataaaggaaa caaaaaatat gaacagcgtt tgcaggaaac gatagaagaa atcgagtaga
    10621 aactgattct tttacacaaa ggaggttgtg tcagaagtgg gcagcttcaa gaaataagaa
    10681 ggaatttaag aaaattgctt ttcaaatttt tgtgaaattt cggcttattt ccgaagaagt
    10741 tacttctgga acaccgtgta tcagaggttg tgaaaaagct gccttgcatc aagtaataag
    10801 aacagccaag taaaaacagc tttttatgtt tttaacttgg ttggacttat taccgcagat
    10861 gcaagctttt gagcaccgtt tattcggaaa aaggaactgc gacaagactt ttgtcacagt
    10921 tccctttcct acgattagtc aagtaaaagg tgtaagaaaa atagctcata aacgaaagcg
    10981 acaaaatcaa ggcacaccaa ataaagcccc gttcgtaaaa aattttcgat ctgggcaaag
    11041 aagtggtata ctgtaagtgg tttagttaat ggtccactga gaccgcgtac gcatagtcgt
    11101 actctgtatg tgccctaacc atagcgtgca tacatttcag aagcttgttc atacaagcaa
    11161 ctgtggcaac cttatctttc ttagggatag gttgcttttt taatttgtag taataatcga
    11221 caatatgatt aggagctgcg cgttgctggc gaatcatatt tcgaatggtg aagaaaataa
    11281 ttttccgtgc tttgggatta ccacgcttat tgatgcggtc ttggccagta tacttacctg
    11341 aatgataccg tcggatgtcg attccgacaa aggcattcaa ttgtttatgg gttttaaatc
    11401 gtgtgatatc tcctaattca ccgagcaata aagcggctgt gagtgcgcca atcccaggga
    11461 acgtttggta caacaaaaag caaggagagc cttcggcttt tttgatcatt tgtgtcgcca
    11521 gtgcttcttt ttgttcgagt aaatcttgga gaagttcagc gtaatagatc gttttttgac
    11581 agtggatact gtctttttca acagctggat aggcacactg tgcatagtgg atgatttgat
    11641 ctgccttttg ctcggcacga ttcgcggaga tttttttccg tgtttcgttg atcagtttat
    11701 tttttatttt tgtccgagta gatgccaata caaaatctgg atggggaaaa agacggatca
    11761 atatcaatgc gtaaggggtc agcctgttta aaaagaactg ttcaagctct ggaaaagtca
    11821 actgaaggca gttgtgtaaa tccatgcgca gacgagtgat tttcttctca atttcttgat
    11881 aaaaacgggc taaatcgcgc atctctaagt aaaaattgct ttgtacttct tttggctgac
    11941 gttgattttt aagatgagtc tgtgcgagcc gatgggcatc cgatttatct gttttccaac
    12001 tgcataaggt gttttcttcc atctgttttt tcgcttctaa cgggtttaac aaacagtaag
    12061 aaaagtgatg atcttgaaag aacttttcga ctggtctgga ataaatcccc gttgcttcaa
    12121 aaacaatcaa tggacgttca ggcaagtgct ggatctcttc gagtaatgca tgaaatcctc
    12181 tttgagtgtg ttcgatctcg tcttcaaata agcaggtgtc gtctttgtat aggacactgt
    12241 agctgtgtcc cattgaaaca tctaatgcaa gaatgtaagg catttgtttt cctccttttt
    12301 aagttttgaa agaactttac tgcttcattt cgattccaat ttcctataca cgggctcatg
    12361 gccccaacat acgtaaacct gattctaaaa tgacagtaaa gaagttcggt ttttgatagg
    12421 gactcatagt cctcaaggct ttgttcgaac tttctttcac tcttcactat aatctaaaaa
    12481 tagaaaatag aggagaacat cattcgtcaa tgatgttctc ctctaatctt agtatgtttt
    12541 tatttggaag ataaagcttt tttcagattc aatttactga aaactagcat gcttgttact
    12601 acgatgaatt gcgttaatgg cacgcctgcg ccagtgtaga cacttaaaaa ataatccttt
    12661 ttttctggcg cataagaaca aaaccacgag acgccgaaaa gaaaaaccaa ttgtgtcagt
    12721 attaatccgg tgactacaat caaagaggaa ctataatagg cacgatcagc ttttgcaaat
    12781 gcggttccta gtatcataag taatatccct gctattgaag caatcagcac gggaatggcc
    12841 agaacttggt aaatttttat catgacattc gcgacttttg ctgtttttgt caactggaaa
    12901 tcaggtgttc tcggatcact gctatcgatc caattatcca ttagtggaag attcaataat
    12961 tcttgggcct gttcgacttg ttcttgcgta ccgaaactat aattcccccc ttgatggaaa
    13021 tctttgtatc gccatgcata ggcatatcct gatttcagaa aagcaaatac gattggcaga
    13081 tcattcatcg tctttccatc ccctgatccc atcagataga attctttttt ctttttgata
    13141 gttccctttt gataggcttc ttctaattct tgattgactt ttttccaaaa atcattggtt
    13201 tcttttgcat tgttttggta ataaccagat tcgcttacca catctcgcaa tgcccaaaaa
    13261 atgatatctc ctggtaattc ttttactttt ccaccttggg tccacgcatg agtcgtatac
    13321 aattcgtcaa tacgagtaga aacagcatga aatgtaggag atgcgtctaa tgctttattt
    13381 aatgcagcat gcgagaccca tatctcactg ttttctttat ttaagttcgt ttcgtcatct
    13441 attcggatca gctgtttcat caaacgtcca aattcgccac tcgaacggtc attgatggcg
    13501 gcaatactat atgttgtctc gtttttatat tcgatccact gatagacgat ggagaaaccg
    13561 actagaggga gtaaaaacaa tgaaagtttc agccattctt gtttcgaaag gagaaatggt
    13621 cgttctttta acttctttgc ttctttgaaa gaaaagtctt gtcctagcaa gatctggacg
    13681 aaactgatgc ctaacgcgac tacagtaaaa ggaagaagcc agatggaatc ttcgcgaatg
    13741 aaccaaaaga aaggaaaaga aacagctagc cctaaaaacc acggcagcat ttttttgctg
    13801 gaactatatt tacggtaata aatagctagt aaacaagcga taatgatctc aacagccgga
    13861 atcacgaggg cattacgata cgttcgagtg gaatattcat gtgtaaaact aatcggagaa
    13921 aacaaaaaat aaaaatagaa acttaaaaga agatacttgt tcttcacgat cggtttaaac
    13981 gcgagtgcaa ccaatatact ggcaaaaata ctgaacatgc cgaacaaaac tgcataagaa
    14041 agatgcagtt tatgtgcaag tactaaaaat aagctgtagg agattccttt ggaaagtgtt
    14101 gtagtcgtat actcccctag ccatttaccg tccaaaatat tttgagcata gcgaatcagc
    14161 agctggtcat cataggttgc cgacatgtct gttccataga ttgctttact atataaatac
    14221 aaacgaataa ttgtaaataa tagggctatg ccaagaataa agtatgtatg tttctttttt
    14281 agttggttga tcaagggttt taaagtgttc agcatctttt cgtccttcca atcacgtttt
    14341 gccacttgct tgtgaaactt ttcgtttttt tacttttcta ccagtatacc ctgtttgtct
    14401 aaatttaaaa agagggggga gaagatgcat gatgaaaaag actgcaaaaa gcactccttt
    14461 gtttcttaaa caaaaaagga caaggcaatg tgcctggtcc ttttttgctg ctgtcagata
    14521 gaaaatctta gagtatcact tcttctgcta ataactcagg aaacacttct ggatcgaata
    14581 tttgtgcttt gaacatggcg atttcttttc catatggtgc gtaagctttc tttttatctt
    14641 tgccgacata gggagtttcc aagattttcg aaatattgtc aaacattgga tcatggacga
    14701 tcttattcag cgtgtcgaat ccgattgttc caaaaccaat attggcgtgc cggtctttat
    14761 gcgaaccttg cggattttta gaatcattga tatggagtac tttaagccga tctaacccga
    14821 tgatcttatc aaactcttcc attacacctg aaaagtcttc tttaatatta taaccggcat
    14881 cattcgtatg acaggtgtca aaagtaacgg ataatttttc attcaatttt actccatcga
    14941 tgatttgcgc taattcctca aatgttcgac cgatttccgt acctttacct gccattgtct
    15001 ccaaagcgat ttgtgccgtc tgttcttttg tcaaaacttc attcaagcct tcaataattc
    15061 tagcaattcc ggcttctgtt cctgccccta catgagcacc tgggtgaagt gtaatttgag
    15121 tagcgcctaa agcttccgca cgaacaatct cttctctcag aaattggacg gcaaaaggaa
    15181 acatttctgg tttgatcgta ttccccaaat taatgatata aggggcatga acgacgatat
    15241 ttgttagacc gttttctgcc atgtacgctt ttccggcatc aatattcatc tcttcgatgg
    15301 cttttcttct agtgttttga ggggcgcctg tatagatcat aaaagtcgaa gatccataac
    15361 tggctgcttc ttctgctgct cctaacagca ttttctttcc gctcatgctg acgtgtgagc
    15421 ctaataacat ggatggattc ctccttgaaa ggcaattgtt actaacaatt cctatatatt
    15481 ggtaagatga tatacaaaga aaatgattga tttttctttg ccctgttctc catagtaact
    15541 gaaattttct ctaaagaaaa gcttaagaac tcttttataa aaaagatcat tttctcacgt
    15601 tttaactctt ttaaaaaaga aattcctgcc aaaaacagcc ttgtctgttt ttttgttcta
    15661 ttatttgcta taattagcga aataaaacca ttagttgagg tgaccctttt gccacgcaaa
    15721 aatactcgaa aaaaacagaa tagaagaaaa aaagaaaaat ggtttgttcc taagattatt
    15781 tttagagttt ttcaatcatt gactgttttt attactgttc ttcttttgat gttcgcagct
    15841 ctaggtattg gaataggcgc tggctatttt gcctatctcg tcgaagacac acaattgccg
    15901 actaaaaaag cactgcagac agaactagga aacattacag aaacgtcaaa gattgtttat
    15961 gcagataata cagagatatc aaaaatccag acagatctga tgcgcacgac tatttcatct
    16021 gataaaattt cacctttatt gaaaactgca attatcagca cagaggatga atacttcgat
    16081 aaacaccaag gttacgttcc taaagctgtt ttacgagcat tagtatctga agctactggt
    16141 attggctctt ctggtggatc aactttgaca cagcaattag tcaaacagca gatccttaca
    16201 gatgaaacaa cattcaaacg taaagcaaac gagatcattc tttctgcaca agtagaaaaa
    16261 tacttttcaa aagatgagat cattgctacg tatttgaatg tctcaccatt tggtcgtaat
    16321 aataagggac aaaacattgc tggagtccag gaagctgcaa gaggaatctt tggtgtagat
    16381 gcaaaagatg tgacgctgcc gcaagctgct tatatcgctg gattacccca aagcccgatc
    16441 acgtatagtc catacacgaa cacaggtgcg ctaaaagaag atctttctgc cggccttgcc
    16501 agaaaagatt ttgttttatt cagtatgtat cgtgaaggcc aaattacgaa agaacaatac
    16561 gaagaagcaa aagcctatga ccttacaaaa gactttctac cacaacaaat cgcagaacag
    16621 actgacagag aattcttgta ttatactgtg atgaatgaag caacaagaat cattgcacaa
    16681 caattggctg aaaaagacaa cgctgatatg tctgattcat cggtcagtga cgcttattat
    16741 caaaaggcac agcaaacgat ccaaaataaa ggatatacaa tccattcgac aatcgataaa
    16801 gatgtctatg ctgcattgca aaatggcgta gaaaattatg gatacatgtt agatgatggt
    16861 gctggcagtc aagtagaaac cggaaatgta ttgatggata acagaactgg ccgcatctac
    16921 ggattcgttg gcggacggaa ttacagtcag aatcaaaata accatgcctt cgatacagaa
    16981 cgtcaagctg gctcatcaat caaaccagtt cttgtttatg gacctgcaat cgacatgggg
    17041 cttatcggat cagaatcccg tgtatcagat tatgcaacaa cttggcaaga aggagaaaat
    17101 gctggcgaga aaatcgtcaa tgctactaac gaaggatcga acaccttcca aaccgtccgt
    17161 gagtcactag aatggtccaa caatatcccg gcttatcact tatatcaaga tgtgttgaat
    17221 tccggtggat cgaaacaata tgcttacgag cactacttgg caaagatgaa ctacccggca
    17281 aatgacaatt ggggcgttga atctgctcct ttaggtacag tagatgtcac gacgcttcaa
    17341 cagaccaatg gatttcaggc tttagcaaac ggcggcgtct atgaagaagg ctatatcatc
    17401 gattccatca cagataacgc aggaaatgta atttataaac acgaaagcaa ctctgtccgg
    17461 atctattcgg aagcaacggc atcgatcatg aatgacatga tgcgcagtgt gatcaatgca
    17521 aaaatcacta caccattcaa agatgccatc tcaagcttaa acggaaatct aggaaaagca
    17581 gattgggtcg gaaaaactgg ttctaccaat gaataccgag attcatggct aattgtctct
    17641 actccgtcta ttaccatcag tagctgggca gggcacgatg acaatactgg aatggacagc
    17701 aaagcaagga tacgttcggc taattatctt gccaatctga tcaatcaagc ttaccaagca
    17761 aaacctgaca tttttggcac tgatgagaaa ttcgaattat catctgatgt gatgaagaaa
    17821 aaagtatctg gatttactgg tcaaacacct ggaaaagtta ctgtcaataa aaaatccatc
    17881 caaacaccag gaaaaacagt tgaatcgcta tgggcgaaaa atggaccgaa gaaaagcgcc
    17941 tttaaatttg gcgttggcgg taccgatgaa aactatacag attactggaa caccgcttca
    18001 gcttatgcaa gagcgaatca agcaaccaaa gaatcagatg agaaagatga ttgatttaat
    18061 agaatacaca gtaataaggg actgtgacaa tagtattgtc acagtccctt atttttttga
    18121 tggtggtgaa cataatgcag catctataca cggattgttt tatatacgat aaatgtaatt
    18181 tcaaccactt tctctctttt ttaagacaaa aaaaaaggat taaacaaaat tttgtcataa
    18241 ccccttttcc cgaataaacg gtgttctcct gccaactccg aatcacgagt cacaatattc
    18301 atcaaacatg aaatgctgtt ttttgaaatt gctccttgta acgcagagtt aaacggcagg
    18361 ctcacaacct ctgaatggtt gtccttttat tattttgtag atcctttttc ttcgattcca
    18421 taaggcaaga taatcgtttt ttcttcgact tcttctttat tcatcatttt cgtcaatagg
    18481 cgcatagata ccgcccctaa atcgtataat ggttgtgtga tacttgttaa acgaggacga
    18541 gctacttctg tcagtagtga attgttactt gtgatgattt cgaattcttc cggtactttc
    18601 acgccacggt ctaataaacc atccaaaata ccgatggcta attcatcatc tgaaacatag
    18661 acagctgttg caccgctatt atggatccga tcgactagag atagaccatc tttgaagttg
    18721 tattgtgctt caaagatcaa tccttcgcta tatggtaggt tattttcgct taatgcttct
    18781 ttatagcctt tcagacgatt ttgtccattg ataggatcga tcaacgcacc actgacaaaa
    18841 gcgatttttt tattgccgct ttttgccaat ttgtttactg catcttttgt tgctgataca
    18901 taatcaatgt ttacgctacc tacttgttca tctggatcaa tagagccagc taatacaaca
    18961 ggagtttttg aacgtgaaaa ttctccacgg atttcgtctg tgatatggtg acccatgaag
    19021 atcacgccgt ccacttgctt agccagtaag ttattcaata cattgacttc tttttggtcg
    19081 ttaccatctg aatttgctaa gataatattg tatttgtaca ttgttgccac atcatcgata
    19141 ccacgtgcta aagaagcaaa gaacatattg ctgacatcag ggatgattac cccaactgtt
    19201 gttgttttct tacttgccaa gccacgcgca actgcgtttg gacgataatc caagcgatcg
    19261 ataacttcca agactttttt tcttgttgct ggtttgacgt ttggattgcc atttacgaca
    19321 cgtgaaacag tagccatcga aacatttgcc tctctcgcta catcatagat agtgattgtt
    19381 tgcttttcca tttgtgttct cctattcata ttttatatgt tttctgaaaa tacgtcttac
    19441 atttctaaaa tagaatatca aataatctca agaattgcaa ccgttttatc tctgttttca
    19501 tgaaaatttt tacttttttc agtttttaga acgttggtct tttaataaat cagtggtaca
    19561 attattgaat acaaaattaa tttagaaagt aggttgccaa atgaataacg taaaaatcaa
    19621 tgaactacgt caatggatgg cacaacacca agtcgaattg gcttatatca gcgaccctgg
    19681 ccacatcgct tacttttccg gatacgaaag tgatcctcat gagcgtgtac tagctctttt
    19741 tatccctctt gaacaagatc ctttcctttt cactcctgct ttggaagttg aagatgctca
    19801 aaacagtagt tgggcgtatg atgtccgtgg ctatttagac agcgaaaacc cttgggagat
    19861 catcggaaaa gaacttcgtg ctcgctatgg tcagcctggc aatgtaggga ttgaaaagaa
    19921 cgccttgact gtcgagcgct tcgatgctat ctctgatatc ttgtcgggaa cggattctta
    19981 cactgatcta gcttccatca tccaacgtct acaactgatc aaaactctag aggaaaaaga
    20041 caaactgatc gaagctggta aatgggccga tgttgcattt gagattggct ttaaagcagt
    20101 gaaagaaggc gtgactgaac aagcgatcat tgcagaaatc gaataccaat tgaaaaaaca
    20161 aggtgtttca caaatgtctt tcgacacatt agtccttgca ggagcaaatg ctgcaagccc
    20221 tcatggtaca ccaggaaaca cgaagatttc accgaatgaa cttgtgttat tcgatcttgg
    20281 cgttgtttgg aatggctatt gcagtgatgc tactcgtaca attgcttatc aaaaaccaac
    20341 cgacttccaa gaaaaaatat acaacatcac tcttgaagca cagcttgccg cacaagaagc
    20401 cgtccgtcca ggtgtgacag caggtgaact tgatcaaatc gcacgaaacg tgatcaacag
    20461 ctatggctac ggcgaatatt tcaatcaccg tttaggtcac gggatcggaa caaccgtcca
    20521 cgaattccct tcccttgtag aaggaaatga cttagtaatc gaagaaggga tgtgcttctc
    20581 tcttgaacca ggaatctata ttcctgaaaa agtaggggta cgtatcgaag actgtgtata
    20641 tgtgacaagc gatggctgtg tgccattcac gactacacca aaagaattac tagttttaga
    20701 aaaataagag taaaaaaggt gagagctagt ccgctcccac ctttttattc tataaatttg
    20761 tgattttagt gattttagtg atttgtagat gaatctgtca ttgtatcttt tatttctttt
    20821 ttagcttctt tcgcatcttc ttttgccatt ttcatcattt cttttgtgtc atctgcaact
    20881 ggttttgctt ctgacattcc ttcattgaca gtatctcctg ctttaccagc agcgtctttt
    20941 acatcaatca tgatatcttc tgaagtatct ttagcaatac ttcctaattc atctgcaggt
    21001 tttttcatat cttctgccgt ttttttgaaa cgattagata agtcagctga ttgttcttta
    21061 agatttaaca agatatcgtc agattcactt cctgcttcat ctgctgattc acttactttt
    21121 tgtttgacac tacctgctag atcacctgct tgttgagaaa gatcttgcgc tttttctttt
    21181 gcaatacctg ttaactcagt cgatttttct ttcgccatat ctgtgtaatc tgaagccata
    21241 tccatcaaat catccgcttg ggcagctaat tcatcccgaa gttctttacc agattttggt
    21301 gctaacaata acgcggcgat tgctgcggct gttcctccga ctactgcacc taataaaaat
    21361 ccaccttttt tagccatttt tattttcctc ctgtcgtttc agtatgtttt ttgtttttaa
    21421 aaaatttcat cgctgtccca ccgacttttc cggcaaccgt cgctttagct gtcgttttac
    21481 cgattccgcc gactttcgtc gcgatgtttt tactagaatg attaagttct gatacacttt
    21541 cacttaaatc agcaactgct gtgaacaatg gatcgatcgt tgcgactttt ccattgatat
    21601 ccaagagcag ttcattactt ttgaccagca agccttctac ttgtctagac aaaagatcta
    21661 cgtcttttgt gacgatttcg atcgtcgtat ttgcttctgc tacagtctga tctacttttt
    21721 ctaaagtttt agaaacttgc aacaacacac gaacgataaa aacgacaagt atcgcaaagg
    21781 cgattgctgc gatcaatcct gcaatttctc ctcctgacat gatcaaactc ccttcttgtc
    21841 tctcttttct ataaggatag cattttcgtg ttttttaaac aaataaaaac gagcttcaac
    21901 tactttttat tcaattagca gctgaagctc gttagaaaaa agggttattt tttcttttct
    21961 tctttgattt ctgttattcc ttttggatta tgatcgatct tgcttccttc tgcttctaat
    22021 tttttaccta aagacaggat atactcacgt aaaggtgcac ctacttctgg atgtgttaga
    22081 ccatattcaa tacttgtttt cataaaacca aatttgtcac cgacatcgta acgtttacct
    22141 gtgaatttac gggcaaatac gcgttgtgtt ttattcaatg tatcgattgc gtcagttagt
    22201 tggatttcat ttccggctcc tggttgttgc tcggacaata catcaaaaat ttctggagtc
    22261 aataagtaac gtccaataat agctaaatca cttggtgctt tgcttggatc tggtttttca
    22321 acaaagtttt tgacattgta taacccatct tccaatactt ctcctggatt gataattccg
    22381 tattttgaag tttcatcatg aggaacttgc ataacggcga tcgtagaagc atgagtacgt
    22441 tcgtaatcat cgattaattg ctgtgtcaat ggtacttcat cttccatgat atcatcgcct
    22501 agcataacga caaacggttc atttccaata aaggctcttg cttgaagaac ggcatgtcct
    22561 aaaccctttg gatgagattg acggatgaaa tgcaagttga catctgtcgt ttcttctact
    22621 aatttcaata attcggtctt tcctttttca cttaggtttg tttctaattc aatatttgaa
    22681 tcaaagtgat cttcgattgg tcgtttagct tttcctgtaa cgatcaagat gtcttcgatt
    22741 cctgatttca acgcttcttc aacgatgaac tggattgttg gtttatcaac aattggcaac
    22801 atttcctttg ccatagcttt tgttgctggt aaaaatcttg ttcctaatcc tgccgctgga
    22861 ataactgctt ttttgacttt catatacatt cgctccttca aaaaattgtt aatgagaatt
    22921 cattttcttt tttcccatca cgtaacatga tttcttttgc ggctgttttg atttcttttt
    22981 gttcatacag cacttcatag attgtttgag tgatgggcat ttcgatttcc attatttggg
    23041 ctagttccat cgctgcttga gtagtattca caccttcaac gaccattccc atattggcta
    23101 gcacctcttc taggggctgc ccttgtccta acaagttgcc tgcgcgccag ttcctagaat
    23161 gaacacttgt gcaagtaacg actagatcgc cta
//
LOCUS       DAYLZO010000034        23148 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.034, whole genome shotgun sequence.
ACCESSION   DAYLZO010000034 DAYLZO010000000
VERSION     DAYLZO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.034"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(48..1514)
                     /locus_tag="ACGVJG_001201"
     CDS             complement(48..1514)
                     /locus_tag="ACGVJG_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFJ6557173.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="ACGVJG_001202"
     CDS             1901..2497
                     /locus_tag="ACGVJG_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFJ6557174.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="ACGVJG_001203"
     CDS             complement(2555..3037)
                     /locus_tag="ACGVJG_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557175.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="ACGVJG_001204"
     CDS             complement(3143..3340)
                     /locus_tag="ACGVJG_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557176.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="ACGVJG_001205"
     CDS             3635..4393
                     /locus_tag="ACGVJG_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFJ6557177.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="ACGVJG_001206"
     CDS             4453..4881
                     /locus_tag="ACGVJG_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557178.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="ACGVJG_001207"
     tRNA            4927..4999
                     /locus_tag="ACGVJG_001207"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="ACGVJG_001208"
     CDS             complement(5149..5520)
                     /locus_tag="ACGVJG_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFJ6557179.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="ACGVJG_001209"
     CDS             5735..5920
                     /locus_tag="ACGVJG_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557180.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="ACGVJG_001210"
     CDS             complement(6003..6191)
                     /locus_tag="ACGVJG_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557181.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="ACGVJG_001211"
     CDS             complement(6215..6478)
                     /locus_tag="ACGVJG_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557182.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6636..6893
                     /locus_tag="ACGVJG_001212"
     CDS             6636..6893
                     /locus_tag="ACGVJG_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFJ6557183.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="ACGVJG_001213"
     CDS             complement(6964..7155)
                     /locus_tag="ACGVJG_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557184.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7296..7616)
                     /locus_tag="ACGVJG_001214"
     CDS             complement(7296..7616)
                     /locus_tag="ACGVJG_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557185.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7639..8073)
                     /locus_tag="ACGVJG_001215"
     CDS             complement(7639..8073)
                     /locus_tag="ACGVJG_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFJ6557186.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8408..9046
                     /locus_tag="ACGVJG_001216"
     CDS             8408..9046
                     /locus_tag="ACGVJG_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFJ6557187.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9370..9453)
                     /locus_tag="ACGVJG_001217"
     tRNA            complement(9370..9453)
                     /locus_tag="ACGVJG_001217"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9417..9419),aa:Leu,seq:cag)
     gene            9981..10481
                     /locus_tag="ACGVJG_001218"
     CDS             9981..10481
                     /locus_tag="ACGVJG_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6557188.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10671..11015)
                     /locus_tag="ACGVJG_001219"
     CDS             complement(10671..11015)
                     /locus_tag="ACGVJG_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFJ6557189.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11169..12089)
                     /locus_tag="ACGVJG_001220"
     CDS             complement(11169..12089)
                     /locus_tag="ACGVJG_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFJ6557190.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12276..12767
                     /locus_tag="ACGVJG_001221"
     CDS             12276..12767
                     /locus_tag="ACGVJG_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFJ6557191.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12791..15166)
                     /locus_tag="ACGVJG_001222"
     CDS             complement(12791..15166)
                     /locus_tag="ACGVJG_001222"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFJ6557192.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15492..16118
                     /locus_tag="ACGVJG_001223"
     CDS             15492..16118
                     /locus_tag="ACGVJG_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFJ6557193.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16141..16845
                     /locus_tag="ACGVJG_001224"
     CDS             16141..16845
                     /locus_tag="ACGVJG_001224"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFJ6557194.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17037..17870
                     /locus_tag="ACGVJG_001225"
     CDS             17037..17870
                     /locus_tag="ACGVJG_001225"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFJ6557195.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            18067..19995
                     /locus_tag="ACGVJG_001226"
     CDS             18067..19995
                     /locus_tag="ACGVJG_001226"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFJ6557196.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            20133..20588
                     /locus_tag="ACGVJG_001227"
     CDS             20133..20588
                     /locus_tag="ACGVJG_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFJ6557197.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            20668..20796
                     /locus_tag="ACGVJG_001228"
     CDS             20668..20796
                     /locus_tag="ACGVJG_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557198.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            21058..21327
                     /locus_tag="ACGVJG_001229"
     CDS             21058..21327
                     /locus_tag="ACGVJG_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557199.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            21727..21981
                     /gene="rpsT"
                     /locus_tag="ACGVJG_001230"
     CDS             21727..21981
                     /gene="rpsT"
                     /locus_tag="ACGVJG_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFJ6557200.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(22070..23116)
                     /gene="holA"
                     /locus_tag="ACGVJG_001231"
     CDS             complement(22070..23116)
                     /gene="holA"
                     /locus_tag="ACGVJG_001231"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFJ6557201.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
BASE COUNT         7193 a         4445 c         3947 g         7563 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgagg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac tcctcgaact
     7261 aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat ttcattctgc
     7321 tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata atcatagctg
     7381 tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc gttagctgtg
     7441 acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac accttttttt
     7501 cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc gtcttcaata
     7561 attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg aaccattttc
     7621 tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat gtgatacgac
     7681 ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg tcgctcccat
     7741 aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat ttcttggatc
     7801 tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat cattgatttt
     7861 tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg aaaaggcata
     7921 ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt gggcttcatt
     7981 gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg atccgtcaac
     8041 tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt tcttttattg
     8101 taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat gatgaacaaa
     8161 agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct gttctaatcc
     8221 cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga acatcattct
     8281 gttacaaaaa acgtggattt gtcataaatt gtcatgcttc ttttacctat gaaatatttt
     8341 cacgtgttaa agtattcctc gttgaacaca acaaataaaa ctttttaacg aataaaggag
     8401 atttttcatg acatcactta aaactttact ttttggaaca actttggctg ccggcgctgc
     8461 attcttcatg ggaacgactg ctcatgcgga cgaagcttat accgttcaat caggtgatac
     8521 tctatcaacg atttctcaaa aatacgttgg tgataactcc ttgatcaatg ccattgcaga
     8581 atcaaattca atttctgata ttaacctgat ctactcagga caacaattaa caatcccaac
     8641 agaaggctct gcacaagcag ctgctgaacc tcaagcagcc gttcaagaag caccagtcca
     8701 agctgaacct gttcaagctg aacaacctgt tgtacaagaa acggttcaaa cggaaacaca
     8761 agcagcgcct gttgctgaaa cacaacctgc tcctgctgta acagaaactg ctgctactcc
     8821 agcaagtaca agctctgcaa aagaatggat cgcacaaaaa gaatcaagcg gttcttacac
     8881 agcaacgaac ggacgttaca tcggccgtta ccaattagat tcttcttact taaatggtga
     8941 ctactctgct gcaaaccaag aaagagtagc agaacaatat gttacttcac gttatggttc
     9001 ttgggaagcg gcgaaagcat tctgggaagc aaacggttgg tactaagatt cattatttga
     9061 acgtttaaac acattcaaaa tatagtaaga ggtcatgcga tttattcttt cgcatgacct
     9121 ctttttgagt ttaagtttag tctttattcc ttaactatcg tttgccagta tttcttgtat
     9181 cagaatctat aaatctacta tttgaatcaa tctattaggt tttaaactgt cccaccttca
     9241 cacaaatttt ttttgattta tttgaatctg gttccgttgt ttaaaatttt tcctatattc
     9301 ttttagataa tatgttattt tctttctcat cgctccaggt gtaccttctc tttataaaaa
     9361 aacaaattat gcggctaaga agactcgaac ttccacgagg tcaccctcac tagcgcctga
     9421 agctagcgcg tctgccaatt ccgccatagc cgcatcacat cttttagttt aggattacat
     9481 gtgttttttg tcaagaacat atttttgttc tttttcattt ctgtacgccc tcccttatgc
     9541 catctacaca aataattcat ccgagcaact ccatcatcta ttgagggttg gtgaaaaaat
     9601 ctgttttatc catatgatcg atttcaatgt taaacgaacg actctgggat tagtaactat
     9661 tttatttttt tcttcatttc ttttttcctc tttttctcga ttcagctatc ttctttcatt
     9721 tggatattga ttatttcttc tgttgattac tatccttctg gaattctaga cgctgaagta
     9781 attcacgttt tctttcttct agagatcata agtaacgaat gaacgaactt caaataattt
     9841 ttttattgta attcatattt gtattgttcg atgattctta atacgatatg acacaataaa
     9901 taaaaatagg catggctatt tacaacaata gtgctatagt agaaaacaaa tgaataaggt
     9961 tacttaaaga ggtgaatacg atgaaactga tcaaactatg ggaagcagat ttaaagaaag
    10021 cttatcagct acaaactagc tttggaccaa atgagagtgg atttatcaac aatgcttatg
    10081 gctatacatt tgctgaattt ttagattatg taaaaaaatg cgaaaaaacg tctttaggaa
    10141 tcgatcttcc tgagagttac gttcccgcaa ccgtctttat tttagaaagt gatgaacatg
    10201 agtatgtagg aatttttaat ttgcgccata aactgaacga aacattaaga aacggcgctg
    10261 gccatattgg ttacggcatc tcccctaatt atcgtaaaaa aggatatgcc acacaaggat
    10321 tagcattagt actacaagag gcaaaaaaaa tcggcattga tgaggcactt ttgtctgtaa
    10381 ataaaaaaaa tcttggcagt cttaaagcac aacaaaaaaa tggggctgcg atttatcgtg
    10441 aagatgagaa agaatactat acgaaaatca aactgatgta aaaaagaagg attccaaaag
    10501 gaaagggatt tgaatgtcct ttatcaatag aacacattta tttaattaac ttataatata
    10561 gcagacaaaa ttttataaac aaagaaacct tttatctctt ttcttctaga aaggataaaa
    10621 ggcttttttg tttttcctat ttgcttctca tagctagtca gccaagattt ctatcgtatc
    10681 cccaacgcaa tccgtgcata acggctcatc ttatctgtcg tccatgctgg ataccagact
    10741 agtttgactt ctgctttcgt tacttctggc acttctttta atgcgtcatg gatagattct
    10801 gttaaaatat ctgctaatgg acagcccata gttgttagag tcatcttaat gactgtttct
    10861 ccattttcag gattgaattc tatatcataa atcaagccta aattgacgat gtcgattcct
    10921 aattcaggat caatcaccat ttccaaggca gcaagaatac gttctttgat tagttctatt
    10981 tcttgtccag accgttcttg gtttgcttcg ctcatcgtac gacactcctt aacacgattt
    11041 ttatttatca tactcttttt ttagctgctt gtaaatgaga atttagaagt aagattctcg
    11101 tttaaattta taaaaatgtt cttattagat gtaatagatt aaatttattc taaaataaac
    11161 ctgattcctc attttttttg tgaacgattt tttatttctg gcaatacgcc agttgttcga
    11221 tacatttttt gttcgttttc atttgcaaac cggccaaata ctgaatgagt tggtaaaata
    11281 ttgataaatg catcgtcgtc gatttcatga acgatctgat caagatcata catttcgtag
    11341 cgggtgatga ccatcatgat catgttccct tgtttttttg aatagccacc gacagacggc
    11401 agtaatgtca ttccacgtac catccgtttt gctagttctt ctgttacctc ttctggcttg
    11461 acggtaacga tcattgctgt tactttttga tgactcgtgt ggatggcatc aattacttga
    11521 gtcattgcat aaatggaaat aatcgtatac aaggcacttt cccagttaaa gaagaagcct
    11581 gcgatgacta caatgatacc atttagaatc agcatgtagt ttccgactgt ttttccagtt
    11641 gtttgtgaaa gtacaagaga gatgatatcc atcccaccag ttgtaaagcc catttttaaa
    11701 gataatccta cgccaagacc taataaaaca ccgcctacta gggcattcat caaaatatta
    11761 tcagtgatct gtccgactgg aagcagcatc gtcatcaccg atacacatac aacattcaca
    11821 aaactaagga tcgttgctcg ttttcctact ttgagaaacc ctaatacaaa cacagggata
    11881 ttcaaaagaa aaataaatgt tcctgtgtct atcttgatat gaaaccattc atataaaagc
    11941 gaggaaatga tttgagcgat cccattcatc cctgcagaaa agacattggc tggaatcaaa
    12001 aaataattta aagcgaccgc agcggtcaat ccggttatca aaataaccac tattttcttt
    12061 aatctttcat ttttatgata cgcagtcata attttactca caagcacctc tcccctactt
    12121 tggtattcca aatgtaagaa taacagttta tctattaaaa taaaatactt cgaactcaaa
    12181 tgagaagaat tttagcaaac aggtaagaat ctgccctaat cctatgataa gatagatgta
    12241 tggtaaatat ttatatgtat atcaggaggg ttcctatggc aaaagatgct agcttcgaca
    12301 ttgtatcaga agtcaataca gaagaagtaa aaaatgcgat tcagcaaaca acaaaagaat
    12361 tgtccaatcg ctttgacttc aaggattcta cagtagaaat caaattagag aacagcaatc
    12421 tagttgtatt gagcgatgat gatttcaaaa tcgaacagat caaagatgta ttatttggta
    12481 agctgatcaa aagaaatgtg ccaatcaaaa atatccattt ttccgaatca gaacacgctc
    12541 taggtggcaa agctcgacaa actgccgatt tggtcaacgg aatcgatcgt gaaaacgcga
    12601 agaaaatcac tacagctatc aaaaatgaaa aactgaaagt aaaagcacaa atccaagaag
    12661 atcagattag agtgacaggt aagaaccgtg atgatctaca agctgtgatt gctttattac
    12721 gtaaacttga cttaccaatc gagttgcaat tcactaacta tcgttaatca aaaaacgagg
    12781 ctgacgtctg tcattcctcg tttttttgat ttactgcttc ttttgataac caaaaacgct
    12841 gtattccttc ttccaattcc ccaacacttc gtacttctct tgctgtttgc caatgttgtg
    12901 ggaataaacg tcgataagaa aaactagaaa atcgttgatc tgccaataca acgattcctt
    12961 gatccgccat gccacgaatc actcgaccac cagcttgcaa gactttgttc attccaggaa
    13021 gctgataagc gtaagcaaat ccaaggtgtt ccttctcatc ataatatgat ttgatcagtt
    13081 cctgctcatg attcatctgc ggcaaaccta cacctacgat catactgccg atcaatcggt
    13141 cccctctcaa atcgattcct tctgaaaaaa tcccgccaag tacgcaaaat ccaatcaatg
    13201 tttcagcggg atttgtttga aattttgtta aaaatcgttc gcgttcttcc tcattcatgt
    13261 ccgtttcttg gattagcaca ttgctgtcgg gatattgctg tctgaatgct tccaccgcaa
    13321 gatctaaata ttgataagaa ggaaagaaaa cgaaataatt cccgaccttt ccatcagcaa
    13381 aagcatgaat cgtctctact aattttgcca gactgtgttc cctttttcga taagtcgtct
    13441 ctaggtaatt tgcgatgaaa acttgctggt ttccttctgg gaaaggactc ggcagtttgt
    13501 aagccagact tttttgtccc cccagtgttt cctgataata agagagtggt gaaaaactag
    13561 cagaaaaaag aatactgctt tttcctttat ccaacgactg ctcaagaaac aagcttggat
    13621 caatacaaaa ctcttttact atcagatcgc gatacgtttt ttcgactgtt gtttcgtagt
    13681 gatcatcata aaattcactg accttcaaaa aatgaagcaa atcaaaataa taaggaagca
    13741 attgttcttg tgatgggtgc tcaggaaatt ctgccagcca ttctttaatt ttttcgctta
    13801 attgataacc tgcctttact aaagattcag ccggtgcttt ttgatgatga taatcccaat
    13861 gatcttcttt tgctatttct cggatcgaat caaattcttt taatagtttg ttgaaacggc
    13921 gatgaagttt tttgaattcc tttggtatag cttccttact tcgtttcgtt ttttcataag
    13981 atagttctgc cgaatacatc tctcgggaac gattcaccaa attatgtgct tcgtcaatca
    14041 aaaacagata gtcctcgttt ttttcatctt caaaaaaacg tcttaaataa acagttggat
    14101 caaataaata attgtaatcc ccaatgatta cgtcacacca gatactgaca tctaatgata
    14161 gttcgaaagg acaaagtgta tgctttcttg cataagtttc gatgacttca cgagtgatct
    14221 gattctcatg attcaaaaga tcccataaac cttcattaat ccgattgtaa taaccattgg
    14281 catatgggca ctgatctgga ttacaggtcg tctcatctaa aaagcagatc ttgtctttcg
    14341 ccgtcaaagt gacacttttt acttcagctc ctgtatcttt taatgctgtc atagcatctt
    14401 cagctacttg tctagttatt gttttagccg ttagataaaa gatcttttct ccctcttctt
    14461 cccctacagc tttcaaagcg gggaataacg tagagattgt ttttcctgtt ccggtaggtg
    14521 cttctacaaa caatttctgt ttcgtacgga tcgttttata agcagctacc gctagttcac
    14581 gctgaccttt cctatactca tcatggggga atttcaaagc catcaaagag gcattgcgta
    14641 cagtgcgcca gttttcttga aaaacaagcc attctttata atcttcagtc agctttttaa
    14701 aaaaagtttc taactgctct cttgtctgat gttcaacctt tctcgtgatc acttcttctg
    14761 ccgtctggaa gtaagtcaat tgcagattta tttcttgtaa atcatgttct tgactataga
    14821 tataggcata cacccttgct tgatgaaaga aaagttccac ttgttctggt tctaaatctt
    14881 caaatcgtgg ttcggaggtt ttgatttcat ctatataata aataccgtct cttttgaaaa
    14941 taccgtctgc acggccttca acgatcaggt aatattcatc taattgaact gttgttttta
    15001 aaaagacttc tttttcatag ttttctcccg catctttttg gagcttgcgg tggattctcg
    15061 ctccttctaa tgcagtatgg ttactttttt tacgattgtc gatacttcca cgtcgcaaaa
    15121 taaattctac taagtgccgc acagatattt ttgactgttt gatcatttcc accacctctt
    15181 tctctctaag attacctgaa aaaacctagt ttagcaaaaa gagctctctt tccaaacata
    15241 aaaaaacacc ctgctgatta ggcagggata taggatatag aatttggatt tttggatgaa
    15301 tctaatgtaa caaggacata ttaaaatgct atgaaaactt actttccttc tctttagaat
    15361 atccaaatac ttttcatata gtctgataat tcagataagt aaagagaaat aagtacctgt
    15421 ttttgtttcc tagcaatgaa tgcgttatac taatggatga aagaagtttt ggctatcaag
    15481 gaggaatgca catgcatatc gaaagattaa aaacaggaat gattgaagaa aactgttatt
    15541 tggtctacaa cgaagaagca cttttgatca ttgaccctgg agaagatgcg gaacgaatca
    15601 aacaagaaat caaaaaaaca aaccaacagc cggtagcgat tttgcttacc catactcact
    15661 acgatcatat cggtgcagtg gaacctttac gcgcgtatta tcaaatccca gtttatgtca
    15721 gcccactaga gcaaaaatgg ctaggtgatc cgattttgaa tctttctgga ctcggtagac
    15781 atgatgatat tgctgatgtt attgtctcgc ctgcagaata tgaatttgaa atgaaaactt
    15841 atactttagg aaacatgtct tttcgcgtcg tccctacccc agggcattct ataggaagcg
    15901 tcagctttat ttttgatgat tttgtcgttt ctggcgacgc cctgtttaga ggaagtatcg
    15961 gtagaaccga tctgtataca gggaatttag aacaattact tcatagtatt cggacccagc
    16021 tgtttgtatt acctgatgaa tttgcagtct atccaggaca tggcgatgca acgacgattg
    16081 aacaggaaaa aaggacaaat ccttttttca atcgttaaaa cgacttagga ggagaataaa
    16141 atgacagaaa ctacacttta tatcgtacga cacggaaaaa cgatgttcaa tacaattgaa
    16201 cgagtacaag gctggtgtga tacacccctc acaaagcaag gacaagaagg cattcattat
    16261 ttaggtcgcg ggttaaaaaa tgtcgacttt tcatttgctt attctagtga cagtggtcgt
    16321 gcaatcgaaa ctgcccgtat tattctagga gaacattcta agggaaaaga aattccttat
    16381 tttattgaaa aaaggattag agaatggtgt ttcgggtctc ttgaaggtgg atatgacatg
    16441 gaaatgtggg gtgtgatccc tcgtgtattg aattttaaaa catatgatga aatgtttacc
    16501 acagaagttt cttttgaaca aattgctaat gctgtataca acttagatac cgctgggtgg
    16561 gcggaaactt atgaaacatt gcgagaccgt gtatggacag gatttgaaga tatcgcccat
    16621 cactgcgaaa aaaatggtgg tggaaatgtt ttagtcgtat ctcatggttt gacgatcgcc
    16681 tttttgctta atctgatcga tcctaaacag cctgttcgtg ctgggctttt aaatggcagc
    16741 gtaacaaagg tcacttatgc aaacggtcga ttttctatcc acggcatcaa tgatacgtct
    16801 tatatcgata acgggaaaac acatagtcgc gactcgtttc tataatgggg atattttaat
    16861 ttttaccaga gaatcataaa aggcaaagtc tgaacgaggg ctttgtcttc ttttttctct
    16921 tcgtgtactt atctgcttta tttctcaagt ttttcttaat attaaaaaac agattgggtt
    16981 tctcaaaaaa taacggtata ctataagatg tatcttaatt gggaaggaag gaatttatgt
    17041 tttttgcaaa tattatcaaa gcagtgattc tcggtattat tgaaggaata acggaatggc
    17101 tgcccatcag tagtactggt catttgatcc tagcagatga attcatcaaa ctggatgcca
    17161 gttcacaatt tatgtcgatg ttcaatgtcg ttatccaatt aggggcgatc ttggctgtcg
    17221 tcgttcttta ttttcacaag ttaaacccat tttctccaag taaatcaacg gtagaaaaaa
    17281 aagatacgtg gacactttgg tttaaagtga tcgttgcttg cataccagcc atcatcattg
    17341 gtttgccttt ggatgattgg ttagaagcaa atttccacaa gttcttacca gttgccattg
    17401 ttttgatcgt ttacggtatt gcctttatta ttgtcgaaaa acgcaacaaa aacaaaacac
    17461 ctcgttggac ttctctgaat gactttacgt accaagcagc tcttcttgtt gggttattcc
    17521 aagtcttggc acttgttccc ggaacatccc gttctggcgc aacgatccta ggatctatct
    17581 tgattggtgc ttcgcgtttt gtcgctacag agttttcatt tttcttaggg attcctgtca
    17641 tgtttggcgc aagcggcttg aagatcgtca aatatttagc tgaagggaat tcattcaaaa
    17701 tggaagaaac tgttgttctt cttgtaggaa cattcgtatc ttttgtggtt tccgtcttag
    17761 ctatcaaatt cttactaagc tatttgaaga aaaacgactt taccgctttt ggctggtatc
    17821 gtattatcct tggtatcatt ttgattggtt attggtttat tgcaatgtag aaaaaaccag
    17881 aacacttctc ttatttgaga aatgttctgg ttttttattt tctttaataa taatcattat
    17941 aaataataaa aacaacatca agcttaatca ccaaatgata accattatcg ttctcattta
    18001 gctgttatta ttatcaatta catttttatt atgctatcct tgttttgaca aggaggtcta
    18061 ataacgatga ctaatcagaa aaaagctttt gcagcaacaa tcggctgcct tttatttatg
    18121 atcttcggtt ttatcttcga ccgattctct cttaccatca ccccactttt ttatatcagt
    18181 gccattatca tcggtggata taaacaaaca tcggaaggct tgaaagaatt gtggaatgat
    18241 cggacactga atgtggattt attgatggct ttagctgcta ttggtgcctg tttgatcggt
    18301 aattactttg aaggagcaat gttgacattc atcttttgtt taagcggtgc attagaggaa
    18361 tacacaacta ataaaagtca aaaagaaatt actgctttga tgaatcttca accacaaaaa
    18421 gcccaattat taaaagaaaa tggacaaatg caagaagtag atgtttctca actaaaggtg
    18481 gataacttgg tatttgtcgc taaaggagca gctgttccga tagatggctt tttagaaagc
    18541 acacaggcaa cgatcgatga gtctgcatta agcggtgaat ctgtgcctgt agaaaaatat
    18601 ttaggcgatg agatgtatgc aggaacgctg aatcaaggaa atcccatcac gattcgagta
    18661 acaaaaacga gttccgaaac tgtctttgct aagattatcc aattagtaga agaagcgcag
    18721 aatacgccaa ctcaaacagc ttcatttatc gaacgaatcg aaaataatta tgtaaaattg
    18781 attttgcttg ctgttcctct aatgatcctt ctcccccact ttttcctagg ttggagttgg
    18841 gatgaaagct tttatcgagg aatggtatta ttagttgtgg cttctccttg tgcgcttgtc
    18901 gcatctgcta caccagcttc tctagctgct ctttcaaatt ctgcaaaaaa tgggatttta
    18961 atcaaaggcg gtatccattt agaaaagctt tctgaattaa aagcgattgc ctttgataaa
    19021 acaggaacgc tcactaaagg aaaaccagtt gtaactgaca gctttttctt tgatgaaaag
    19081 gatttggcac aacaacttct tgttgctatg gaacaaaaaa cgacgcatcc acttgctcat
    19141 gcgattttgc aatatttcga ttgcacaata cctgaagaaa tccaatactt accaatagaa
    19201 gaaattactg gttttggatt acaaactacc tatttaggtg cgcaatggaa agttggcaag
    19261 cacgcatatg atcctgagac gatgataatc agcaaagaga ttgcggaaat gatcgaacgt
    19321 ttggaaaatc aaggcaaaac cgtgatttat ctttctaaag atcagcagtt aattgctgtt
    19381 ttaggtctgc tagatattcc aaaagcaaat acccaacaag taatttccta ctttaaatcc
    19441 caaaacatcc atacaagtat gataaccggc gaccattctg gcacagcaaa ggccattgct
    19501 gaacaagtag ggattgaaca ttactatgca agctgtactc cggaagaaaa aacacaacta
    19561 gtcaaaaaag agaatgaact ttaccaagta aatgcgatgg ttggtgatgg agtcaatgat
    19621 gcgcctgctt tagcagccgc ttctttaggt gtcgctatgg ggcaagggac agatgccgct
    19681 atggatatcg cagacatcgt cttaatgaaa agcgacttaa ataaatttgt ctacagtcat
    19741 cggctatctt taaaaatgcg gcggatcatc aaacaaaata ttattttttc gattcttgtt
    19801 attctcttgt tgattgcttc taacttcctg caatttttaa atctacctct tggagttgta
    19861 ggacacgaag gaagcacaat attggttatt ctgaatggct tgcgtttact aagaccttta
    19921 cctgatcttt ctgattcttc aaaaaaatgt aagtcttgtc cactatacaa agcgcggctg
    19981 tcttctgtca aataagcaaa gtgttttttt atgattttgt ttattttatg aaaaaaacag
    20041 cttatgaata ggtcttgaag gatgaattcc tacttttgta actttctaaa aacatgttat
    20101 gattgtttcg aagaaagtga ggaatatatt tcatggaaag ttggcttttt ttaggaatta
    20161 ttttacttgt agcaatttta gctaaaaatc aaagtctgat gatagcggca gcttttgttt
    20221 tgattatgaa gctcatacca gcctcccgtt cctttttccc ttggcttcaa ggcaaaggaa
    20281 tcaatattgg ggtgacgatc atcacagcgg ctattctaat ccctatcgca acaggagaaa
    20341 tcggtctacg tgatctatta gaagccttta aatcccctat tggctgggta gctgtttttt
    20401 gcggtgggct agtggctgtc ttatcttcca agggtgtagg attgattgct tctgatccac
    20461 aaatcactgt tgcacttgtt ttcggcacga ttttaggtgt tgtcttctta agaggaatcg
    20521 ccgccggacc ggtcattgct tctggtatca cttactgtat cttacaagta tttgctttag
    20581 gaaaatagaa caaattttgt ctattttagg agattcttaa cacccccaat ttactgatcc
    20641 gtgatatact gacaacgagg tgatacaatg ttcgaaaaat taaagtattt cataggaaca
    20701 atcggctggt ttcttctagc ggtacttttg attctgggct tagcttatct attatttcct
    20761 gatttgatac aaaatgtgtt tgatactgct ttttgattga cctattaaaa cgatataggg
    20821 gatgctcctg cgatgcaggg gcttttttta atcaatgttt tgtaataatt taggccgtgc
    20881 caatgagact tcctatagaa ttcgatgctc ttttttcttt ttggctcttc tgctcttttt
    20941 tgttatacta gattacagtg gacaaaagaa agaatgatcg catccacttc aacgaatatc
    21001 aaaatggaag aattagtgcc tgttttcagg ttactttgaa aagaaaggaa gacgcttgtg
    21061 gaaacttttg aaggtctttt aattgatacg tacgttttac aagaacagcc aatgatgctg
    21121 actgggatca acttgcagct tgaggaccgt gtgatcaatg gcctgcttaa agatcgctat
    21181 ttggcaaaat atatcttacg tttgccccta aataaatatg tgctggaagt aagtggcaaa
    21241 tggaaccgtc ggaaccagtt ggtcataaaa aaaatgttga taaaaaatat cgatgaatac
    21301 attaaaataa tcggtacacc agaatagcct ttactgatca aaggatactg gttataaaat
    21361 aactgcagtt tcttaataga aaaacaaaga acaacaggga atcgtatccc ttttcaaaaa
    21421 aaactttgca aacggttctc tttgttatat aataacttag taccaaacat tcccattcaa
    21481 actttgttta aatagataaa taaaaatcta gtttggtttg acgctattgc ctcccacaat
    21541 agcgtctttt tgattttttt cagcttgtac tatccgtttt tactgaaaaa aaatcctaat
    21601 aaaagactgt taaggaaaaa aacttgtcat gttcatattg caaagcacgc tgcctttagg
    21661 tataataatg aatgttgaat tatgcggtaa cacgcgtaag aacttaagtt atcggaggtg
    21721 aaactaatgc caaacattga atctgcaatc aaacgtgttc gtacaagcga aaacgctaat
    21781 gtgaaaaatt catctcaaac aagcgctatg cgtactgcaa tcaaaaaatt tgaagatgct
    21841 gtagcttcag gtgctgacaa cgtagatgcg ttatataaag aagctgttaa agctattgat
    21901 atggctgaat caaaaggtct aatccacaaa aacaaagcta accgcgacaa atctcgttta
    21961 agcaaaaaaa tcgctaaata aaagaatcgg cttcggccga tttttttatt tgtcaaaaaa
    22021 aggacaaaaa caaacgacct tcccgcatgc tttgccctcg tctttcttct tatcgatttc
    22081 ttaacaactg ccctgtgcgc aaaacgaata gttgaaaact taattctttt tcgatttgtc
    22141 ccgttttgat ttgataatcc atctcaacta attgatcaaa caactgtacc agcaaatgtt
    22201 catcgaattt ttttacttcc tgcatggcaa gttttacacg atatggatgg attttcaatg
    22261 tttcagcaat atttgcctgc tgataaccaa tctttacaag aaattttgtc tgtaaaaata
    22321 aacgtaattg agaaagcaaa atcgcattaa ttttgattgt ttcttcccct tgcataacta
    22381 aatcttcgta taaacgcaaa gcttgttccg tcttcccttt taaaatatat tgggtcatgt
    22441 caaaaatatt atgttctaac gttttaggaa ctaattcttc tacttctctt accgtgattt
    22501 gctgttgatt ttgtccataa aggaccaatt tctgtaactc tctcatcatt ttggataaat
    22561 ctaagtcggt caacctcaag aataaatcaa ttgcacttcg ttccattttg attgaggaat
    22621 tctctaaggt attcatcaga tattgacgta cttctttttc attcaactgt tggacattga
    22681 tgataattgc tgtttttttc aattgtttcg ttactttttt ccgttcatcc aacttttcat
    22741 atggcgcaaa gaatactaat acagtcgatt ctaatggttc tttcaaataa gccgtcaata
    22801 aatcagtatt ttgttcgatt ccattgttta ctttttcacc tgttaaaaaa taaggattct
    22861 ctacgaatac taaccgttga tctccgaaaa acggcaacgt ctctgcttca gctactacct
    22921 catctaaact tgcattttcc atgtcaaaac ttaaaaaatt taagtcatct tcgccctcta
    22981 attggatttt tttgacaatt tctgtccgaa cttgatcttg gagaaacttt tctgttccta
    23041 aaatcaagta acaagatgca agtttttctt tacgtatttt ttgtaactct gcttgtatac
    23101 tcaaattact caccactttc aaaaattatc ctaccatgag cagatgag
//
LOCUS       DAYLZO010000035        22885 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.035, whole genome shotgun sequence.
ACCESSION   DAYLZO010000035 DAYLZO010000000
VERSION     DAYLZO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.035"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(22..1125)
                     /locus_tag="ACGVJG_001232"
     CDS             complement(22..1125)
                     /locus_tag="ACGVJG_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFJ6557202.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1347..1817)
                     /locus_tag="ACGVJG_001233"
     CDS             complement(1347..1817)
                     /locus_tag="ACGVJG_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFJ6557203.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2150..3790
                     /locus_tag="ACGVJG_001234"
     CDS             2150..3790
                     /locus_tag="ACGVJG_001234"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFJ6557204.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3944..5266
                     /locus_tag="ACGVJG_001235"
     CDS             3944..5266
                     /locus_tag="ACGVJG_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFJ6557205.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5328..5759)
                     /locus_tag="ACGVJG_001236"
     CDS             complement(5328..5759)
                     /locus_tag="ACGVJG_001236"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6557206.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5823..6659)
                     /locus_tag="ACGVJG_001237"
     CDS             complement(5823..6659)
                     /locus_tag="ACGVJG_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFJ6557207.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(7401..8027)
                     /locus_tag="ACGVJG_001238"
     CDS             complement(7401..8027)
                     /locus_tag="ACGVJG_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557208.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8322..8657)
                     /locus_tag="ACGVJG_001239"
     CDS             complement(8322..8657)
                     /locus_tag="ACGVJG_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFJ6557209.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(8644..8928)
                     /locus_tag="ACGVJG_001240"
     CDS             complement(8644..8928)
                     /locus_tag="ACGVJG_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFJ6557210.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(8984..10507)
                     /locus_tag="ACGVJG_001241"
     CDS             complement(8984..10507)
                     /locus_tag="ACGVJG_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFJ6557211.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10500..11675)
                     /locus_tag="ACGVJG_001242"
     CDS             complement(10500..11675)
                     /locus_tag="ACGVJG_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFJ6557212.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(11679..11864)
                     /locus_tag="ACGVJG_001243"
     CDS             complement(11679..11864)
                     /locus_tag="ACGVJG_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557213.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(11830..13524)
                     /locus_tag="ACGVJG_001244"
     CDS             complement(11830..13524)
                     /locus_tag="ACGVJG_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFJ6557214.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(13521..13994)
                     /locus_tag="ACGVJG_001245"
     CDS             complement(13521..13994)
                     /locus_tag="ACGVJG_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFJ6557215.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14063..14218)
                     /locus_tag="ACGVJG_001246"
     CDS             complement(14063..14218)
                     /locus_tag="ACGVJG_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFJ6557216.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14352..14732)
                     /locus_tag="ACGVJG_001247"
     CDS             complement(14352..14732)
                     /locus_tag="ACGVJG_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFJ6557217.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(14736..14951)
                     /locus_tag="ACGVJG_001248"
     CDS             complement(14736..14951)
                     /locus_tag="ACGVJG_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557218.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(14954..15361)
                     /locus_tag="ACGVJG_001249"
     CDS             complement(14954..15361)
                     /locus_tag="ACGVJG_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFJ6557219.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(15627..17102)
                     /locus_tag="ACGVJG_001250"
     CDS             complement(15627..17102)
                     /locus_tag="ACGVJG_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFJ6557220.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17092..17940)
                     /locus_tag="ACGVJG_001251"
     CDS             complement(17092..17940)
                     /locus_tag="ACGVJG_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFJ6557221.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(17977..18339)
                     /locus_tag="ACGVJG_001252"
     CDS             complement(17977..18339)
                     /locus_tag="ACGVJG_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557222.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18340..18498)
                     /locus_tag="ACGVJG_001253"
     CDS             complement(18340..18498)
                     /locus_tag="ACGVJG_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557223.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(18639..19263)
                     /locus_tag="ACGVJG_001254"
                     /pseudo
     CDS             complement(18639..19263)
                     /locus_tag="ACGVJG_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19260..19571)
                     /locus_tag="ACGVJG_001255"
     CDS             complement(19260..19571)
                     /locus_tag="ACGVJG_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557224.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(19615..19908)
                     /locus_tag="ACGVJG_001256"
     CDS             complement(19615..19908)
                     /locus_tag="ACGVJG_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557225.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20102..20749
                     /locus_tag="ACGVJG_001257"
     CDS             20102..20749
                     /locus_tag="ACGVJG_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6557226.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            20810..21955
                     /locus_tag="ACGVJG_001258"
     CDS             20810..21955
                     /locus_tag="ACGVJG_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6557227.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22036..22341)
                     /locus_tag="ACGVJG_001259"
     CDS             complement(22036..22341)
                     /locus_tag="ACGVJG_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFJ6557228.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22366..22797)
                     /gene="ruvX"
                     /locus_tag="ACGVJG_001260"
     CDS             complement(22366..22797)
                     /gene="ruvX"
                     /locus_tag="ACGVJG_001260"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFJ6557229.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(22794..>22885)
                     /locus_tag="ACGVJG_001261"
     CDS             complement(22794..>22885)
                     /locus_tag="ACGVJG_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFJ6557230.1"
                     /translation="ARNLIRRHERDEIMEVIVKDYLSNHGVNL"
BASE COUNT         6626 a         4730 c         3806 g         7723 t
ORIGIN      
        1 atttttttcg tcatttgttt cttaaataaa acgattctca cgcaatttta tttttttgat
       61 atcttctact gtttgtgttc cagctaattg catgaccatt tccaattctg ttttaaagaa
      121 atcaaacact tgatggactc ctgtacttcc tcctaaagac aatccataaa taacgggtct
      181 gccaatagca acaagatctg cacctgatgc gattgctttg aaaacatgct gtccgcgtcg
      241 tacaccgctg tcaaacacga ttggtacacg tttatctact gcttctgcca catattgcaa
      301 cgaatcaaac gctgctggac caccatctag ttgacggcca ccatgattag atacccagat
      361 tccgccagca ccagattcta atgaacgata gacatcttct tctgactgga cacctttcac
      421 atagactggc aaatcagaat gagcagcaat aaattctaca tcttttggac taagcttctg
      481 tttggaagat ttgtacacag catccatcgt ttggcctact cctgattgat aagcctgaac
      541 gatcggcatt ggcagtggga aggtaaaccc atttctgcgg tctgtttcac ggtttccgcc
      601 aactgttgca tccgctgtca atacgattgc ttttgctcca ttacgcttgg ccacttcaag
      661 aatatccaaa ttgatcccat catctttgct catataaaat tgaaaccatt gcggtgcttt
      721 ctccccgcct gcttctctta tctcttctaa tgtacaagaa gcgtaagaac ttgctgtata
      781 gatcgtccca aaatctgcta caccttttgc agaagctttc tccgccttca catgagctaa
      841 gccatgagca gcaactggtg ccatgatgat tggtgcagtc agcgtttctt tgtcaaaatg
      901 tgtagttgta tcgggtaatt ctacatcttt taatacatga ggaataatca gctgatgatt
      961 gaatgcccgt tcattttctt gataagtaaa taagtcgcca gcaccactgc tgatgtaacc
     1021 ataaccacca gctggaatca cttgtgaagc ggctatttct aaatcttcca tattgataaa
     1081 atccactatt ccttcatttg tacctgcttg atacgttttt tccatttcaa tgccttccct
     1141 ctataaaaaa ttgaatgaat aatatgatag cgtttacaca ctttatatta ctcttccgta
     1201 ttgatacagg caaataatct gtttacattt cagtcattcc agttgttact actctgaaag
     1261 aaatgtttag aacacaaata gataaaaaac gatgggactt tgacaaaagt cgtgtcaaag
     1321 tcccttttac gaaatactgt ggatgcctat tgaaataagt gcgggttgtt tctttgtatt
     1381 tcaatcgttt cttgccattc ttcgtttaat tttggattac cgttttcttc cttgaacgca
     1441 taaggtccca atccaaaaag ctcagttagt atttgattca cgattccata tagctcggtg
     1501 tgatcggaca catgattatt gaagatcatc tcattatcac cacgtttgaa tctcacattc
     1561 gggctctcat gatgaacctc tatgcagttt tccccttttt ttaactgacg gatttctgaa
     1621 aacatctcat aaatatcttc tccaataaaa ccttcagcaa gcgccagact gtccacaaga
     1681 ccgcctactg tcaactcaga gattatcgct ttgttggcac caagctttaa tgtatagctt
     1741 gttgcttcta ctacgccgca tgcattgcaa ccaccttctg atagttcgct ttttctgatc
     1801 aattgaactt ctttcatttt ctgttcctcc tcttagtttt ctacttatgt attttttatc
     1861 acctgacgta agtgtatttt atcgtcacga tcacgtgatt tattcttcag agattctatt
     1921 tatctatcta acatatcctt tattcttcta cttttctttc tttagcatat aagagatacg
     1981 catagtaatc aagcaaggat tgttgatata aaaagaacaa ataccgctat tttgatttct
     2041 tttgactcta ttactcatat aaataaaatt tattatacct gctctttttg actatatcct
     2101 catctgactc ctatacttcg tttgtattaa aacttaggag gaatcattca tgcttacagg
     2161 gctaaactta ttaaataatc cttttctaaa taaaggaaca gcttttacaa aagaagaacg
     2221 ggcaaaatac ggaatcactg gtatgcttcc aagtactgtc caaacattgg aacaacaatc
     2281 tgtccaagct tacggtcaat acttaagcaa acagaccgat ttggaaaaac gtatcttctt
     2341 aatgaatcta ttcaatacga accggacatt gttttataaa ttgatgggcg aacatttagt
     2401 tgaattcatg cctgtcgttt atgatcctgt cgttgcggat tcaatcgaac aatacaacga
     2461 aattttcttg aaaccacaag atgctgcatt tctttcaatc gacgaaccag aaaatatcaa
     2521 agcaagtttg aaaaacgcag cagatggacg cgacattcgt ttgatcgttg tgacagatgc
     2581 agaaggaatc ttaggtatgg gtgactgggg tgtgaacggt gttgacatcg ctatcggtaa
     2641 attgatggtc tacactgctg cggctggtat caatcctgca caagtcttgc ctgtatcaat
     2701 cgacgctggt actaataacg aagaattatt gaataatcca ttatacttag ggaatcgtca
     2761 tgctcgtgta gaaggcgaaa cttactacga attcattgat caattcgttc aatcagctac
     2821 tgaattattc ccagaattgt tattacattg ggaagatttt ggacgaggaa atgctgcaac
     2881 gatcttagaa aaatacgaag ataaaatcac tactttcaat gatgatatcc aaggcacagg
     2941 aatcgttgta ctagctggtg tattaggtgg attgaatata tcaggtgagc aattgaaaga
     3001 tcaaacgatc ttgacatttg gtgctggtac tgctggtgta ggtatcgcaa acatcctttt
     3061 agatgaaatg atccgtcaag gtgtacctga agaagaagca cgccaacatt tctatcaagt
     3121 agataaacag ggtttattgt ttgaagatac aaaaggattg actcctggtc aaatcccatt
     3181 cgctcgtaaa cgctcagaat tcactaatgc tgatgaattg acaaacttag aagcagttgt
     3241 aaaagcaatc catccaacga tcatgatcgg tacttctacc caacctggag cattcactga
     3301 agaaatcgtg aaagaaatgg cggctcatac cccacgtcca atcatcatgc ctttgtctaa
     3361 tccaacaaaa ttggcagaag caaaagcaaa agatttgatt gaatggacgg acggaaaagc
     3421 tttagttgga acaggtatcc ctgctgctga tgtggaatat aacggtgtaa cttaccaaat
     3481 cggtcaagca aataatgcgt tgatgtaccc aggtttaggg cttggtttga ttgcttcaac
     3541 agctacacga gtaaatgctg aaatcatttc tcaagctagt cgagcattag gcggaatcgt
     3601 tgatgtaaca aaaccaggag cagctatctt gcctccagta gcaaaaatca ctgaattctc
     3661 tcaaacgatt gctgaaactg tcgcaaaatc agttgttgca caaaaattga atcgtgaaga
     3721 aatcacggat atcaaagaag ctgttgaatc tgctaaatgg gttcctgaat ataaatcttt
     3781 agaagattaa gattgttata gtaaaagaac atacagaaac tttgtatgac gtctaaaaag
     3841 aaaaaagcga ctgcggcaaa acagcagcca gccaactact gctcttttgc cgcttctctt
     3901 tggctttttt catacgatac agaataagaa aggatctgta aaaatggcac aaaaaacaac
     3961 aaccgcaact gcagttgagg aaaagaaaaa agaagggttt tggtcaacaa aaattaccgg
     4021 cgtaagtttg cccctttatt tgatcatggt ccttgtcctg atcattgcaa tggctttagg
     4081 aaaattaccg acgaatatga ttggcccaat cgctgttttg gtcattttcg gtaatttatt
     4141 tcattttatc ggaacaaaaa tccctatcgt caaaagttat ttaggtggtg gctccgtttt
     4201 tgcgatcttt gcctctgccg cgattgctac atttgggatc ttacctgagt atgtagtaaa
     4261 atccacagaa aactttgtca gcaatatggg ctttttagac ttttatattg ctgccttgat
     4321 tactggtagc atccttggta tgaaccgtaa tctattgatg aaagcttctg ttcgttttat
     4381 tcccgttgca cttatttcaa tggtcgtctc cttttttgcc gtaggactcg taggtatgct
     4441 tatcggaaat ggttttgcaa attccgtttt atatatctca ttgcccgcaa tggctggtgg
     4501 tgttggagcc ggagctgtac ctttatcaac gatttatgca aatgttttag gtacggacgc
     4561 ttcttctatc atttcccgcc tgattcctgc gacagcaatg acaaatgtcc tagctatcat
     4621 tggtgcagcg ttagttgctc gtgcgggaca atcaatgcca aaattcaacg gtaatggtaa
     4681 attaatggaa aaaggcgacc ttggcgatgg aaaacctaga aatgtaaaac cttccattcc
     4741 acaattaggt gtcggtttga tggtatcatt gactttcttc atcttaggac aaatctgtaa
     4801 ctattttgtt cctaaagtcc atgcttacgc attcatgatc attatcgttg ttatctgtaa
     4861 aatcgcaaac ctattaccag aatattacga agatgcagct atcatgttca acaacttgat
     4921 tgtcaaaaac ttgacagcag ctgttcttgc cggtatcggt attgcgttat tgaatctaaa
     4981 tgtattggct tctgctttga catggcaatt tgttgtctta tgtttgacaa gtgttatcgc
     5041 gatttcagta gtttcaggat ttgtcggtcg tttgtttggt ttatatccaa tcgaatcaac
     5101 tattactgct ggcttatgca acaactcgat gggtggtaca ggaaatgtag ctgtcttatc
     5161 tgcagctaat cggatggaac ttattgcttt tgctcaaatg ggcaatcggc ttggtggtgc
     5221 catcgttttg atcatctcag gtttcttgat gcaattactt tcttagtcaa aaaaaatacc
     5281 tgatcatcaa gtatcggctt gatgaccagg tattttttga taaaaattta caggattttc
     5341 aaaaatcttt tttgcatttt atcggaatga taaccgatgc gttcataaaa tcggtgtgct
     5401 tctactctgt attctgcaga attaagtctt attgctgata ttcctcgtct tttcgcttct
     5461 tctacgccag tcatcaaggc ctttccgatt cctctttttt cagcatgttt agacacagca
     5521 agtgccataa tattgaacat cggatcgctg aaaatcgatt cataggactc cgcttgtaca
     5581 tagccagctg cttttttaga agtaggatct tcatagacca agaaaaaatt tttttctggc
     5641 tccttcaaca gtttcttcat ttgtctcacc gtaacggtta atggtacatc atatcctaat
     5701 tgttccgcat tgatttctcg tagtgcttct gcatctccta aacgcatttc ccgcaacatc
     5761 caaatcacca gccaattttt atttttttaa atgaattttt ttatcacagt atactccttt
     5821 tttcatttag aacaatatgt ttcagtgatt ttgacttgcg ttagtctaga ttctcctaat
     5881 gtttgtaaga tgacaaaggc attgtaacaa ttgaccaaaa gaaccacgag gacaattact
     5941 tgaaaaataa aggtcacttt ttttggagac aaaaccctgc taaatcttgc tccccacaaa
     6001 cctccaataa cagcagcggg gataacataa aacaacatag ataaatcaaa gcgcataaag
     6061 ctacttgtta aagcaatcgt taccaatttt gccagttgag aaaagaaaat cgtagataga
     6121 gaatatagtg ttgcttcttt tattggcaat gcaaacatca gcatcaacaa agaaacattg
     6181 actggtccac caccaatacc taaaaaacta gctaaaaagc ccagaacccc cccacatata
     6241 aggtaccaag tccatgacgt caaatgaaat tttggctgat gatgcttggt gtagaagaac
     6301 gcaaaaacca aggtcaatac cgtcaaaaaa atttggatca gctgcacatg cttctcgttt
     6361 tcaaataact gtaaaaaaac ttcgaataca atgttgccga ctactcctcc aacaacagct
     6421 ccactagata cccaaccgac aatctgccaa ttcagtgatt tcccgcttga tacttgtcgc
     6481 acagtcgaaa caatggacat cgtgaatacc gcaactgttg agtaaaaaga aatccctgca
     6541 acagaatggg cgccgataag atcaagaatc ggtttgatca acacgcctcc acccatcccg
     6601 gaaatagcgc caactgtgtt cgccagaacg atcaccacaa aataaatcag accaatcatc
     6661 ttgtatttcc tttctcttgt ttatatcttt tagcgattgt tactcttttc ttttattata
     6721 cagtaaaaga aacaaacgag gcttcgaaag cttttctact ttttaaggaa atttagtgct
     6781 aaacgaaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa
     6841 aatagctcct cataattttc gtttggttga acttattacc gcagatgcaa gcttttgaac
     6901 accgtttaag aaagaggtcg tgagcctgcc gttcagctcc gagtcacaag gaacaatttc
     6961 aaaaaacagc ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag ttggcaggag
     7021 aacaccgttt aaggaagaga ctgtgaagtc aacgttttga cctgattatt agagaaacaa
     7081 atggcaaaat agttcttcat atttcgccgt ttttcgagcg aatgccgagg ggcagctgat
     7141 tgaacatcgt ttatttggaa aaagggattg tgacaagacg tttgtcacaa tccctttttt
     7201 tatatcttat taattcattc ttttgttcta ttcttcttct tccgccatga atgagaaacc
     7261 catatcaaag aactataaag gttatcaaaa cagcgtttat gactaaatga attagagtta
     7321 tctatcacta tttttcaagc gatttaaacc aaatggcata caatatggca tacaaaaact
     7381 tcaataagcc gtgggggttt ttaaatttct tcttgttctt taaaatcaat gagtggagct
     7441 acataatttg aatccacttt ttcaaacgtc actttttgca tcgacgaatg ttttctttcc
     7501 caagataacg taaatagatt tgactctgga cttataaagt ctacttcacc ttctagtatt
     7561 actttcttat gcaaatattc ctctaataaa tatagacttg ctaaagcata caacacattt
     7621 tccaaatttg cttccttata atttgaagaa cgaccatgtt ttactctgtt atatattttc
     7681 caccaatctg gtgtatcttc aggccaccca tctccaaaag gttttagtga agtcaatgtt
     7741 aaagaattca taaccctaac accattatcc aaacacatta attcgctaga tttaataaca
     7801 gctttaaaat cagttatgct tttattgcta ctgctatcaa atccgcaaag ttctttgaaa
     7861 ataatctcac actccgaacc aatcgctagt agtagttttt gatattcaat agagaatgtt
     7921 ttgaaattat tctcatcgag catcacaaaa tctgtggtat taataaactc attttctaaa
     7981 tgtagataat acttccaata aaaatttgta aactctgctt tgtccattaa atcacctctt
     8041 ttgaattata acaaaaaaaa cacgtccaca acagacgtgt tctttgattt ctacacaatc
     8101 agcttgccaa aaccatccaa tcagtcctag catgcctgta ttaaaattga acgttcatca
     8161 tctatcatct ttcttagtcc agcatgtttg caaccacacc tacaattttc agtccaacaa
     8221 tagatttcga ctagctaagt cttgcaaaat tcaggcagct aacccttcat ttactacctt
     8281 aattatacta aatacccaca acgtttacaa gtgtaatcat tctaagaaat ctgtttagct
     8341 atgatcgttg tataatcttt aaacgccgtt ccttcattga ttgaaataat tttataactc
     8401 ttgtttttcc aagctacttt catatcgttt gttagttcat aatcttgatc gtatcgaata
     8461 ataaacgtca acgttccttc aagaatggta cctacactag caagcacgtc ttttagcatt
     8521 tgtgaacgca cattagccca acattcaaat acagtttctt cactatccac catctgccca
     8581 tcttcatctt ttacccgttt tgtcttttta aacttgactc tctcagtcaa ttcatttaca
     8641 ttcttaatga ttggcataat aagcacctct catttgttgt actaatgctt gtaccgttac
     8701 tggtatccgt tcgctgctgg cttcttgacg gttcaaatac caatgttgag taagtaatga
     8761 caccgccaaa tcaaattgct gataattatc catatcttta tccgttgcgc tactatcaat
     8821 agcacttttt atatattcgc ctgctgtatc aatcaattgt tgaataaagt catcatcaat
     8881 agtgtgatcg atccgcatac ttttttttat gctatccaaa attaccattt tatctctcct
     8941 aatgaaaaaa aggcagtggg ataaaccgcc gcccaatatt tttctatact tttggtgtta
     9001 caactggcgt aaactcaata tacactgaag cattttgatc aattttctta taatcgttgc
     9061 gaacgatcac tgcaagtcct tgtgagtagt aatcgaattt ttcccattga gtcgttactt
     9121 gatttctacg agcaacaaag atagattgtg caatatcccc cataatcata gggaatgttc
     9181 cagctttagg attggcaaac aatgtatctg caatcaatac tactggcata ccgaataatg
     9241 atttaccact aggtgctgtc acatcgggtt gtaaaatgta acgtccatct gaatctttca
     9301 atgtatctag gtggtaatat ccgctttggt ttagaattac cattttattt aatgctgggt
     9361 ctaatgccac attgtatagt tgtttcaaat cgtccaacgt agcagccgtt ttcttagtga
     9421 atgtttttaa taaatccatg atatgcttat tgtcagtgtt ttctaccaat ttcgctaact
     9481 gatctttgac ctcttgtaca atattcactg ctgaatcttc cacaacctca tttgataagg
     9541 caatcttacc agcacgagtt tctactttat aatcaactga agtaaacatt tcagcgtcaa
     9601 tatcaccaat ttcagcaagt tcagcttttg ttgctaacac tgcttgttgg ttagttgcta
     9661 ctggatattt accttgtcca tttgatactg tttttactgt tgcatattga gctaagttat
     9721 aatttgaacg tttcaaatca aagacttcac cgattacttc ttcgggaaca actgccgctg
     9781 catttacagt agtgactcca tcacgcactt cgccttgtga acggatatat tcttcatacc
     9841 cacgcaattc tgttttttcg ttatcgatta aagttttttc catgttatta ctctcctttt
     9901 gtgctttaga agtggattct aagctattgt ttgtttgttt tgcttggttg ttactcataa
     9961 acgattcgta agaacgctta tttacttgga catttgatga atcataagcg ggaacggtca
    10021 cgacactaat ttcattaagt gctttgattt tatctattga tcgttcaatt gtgccatctt
    10081 cttttttgtc gaatgaatct aatccaagca cgaacccaaa ggacatagaa tcaacaaccc
    10141 cttttgagat attctcatac acatcatttg aataggtcgt gtcggttagc tctgcttcaa
    10201 aatgtaatcc tgtatcatcg acattcaatt tcaacgtacc tgctttgaca ctagctagtg
    10261 gctttgaata atcgtgtcca tataataaga acacatttga taaatccacc tcttttaatg
    10321 cttcgggtgt aatcacttct acaaagccgc ctaaatcttt tgacggttgc ccgaatttca
    10381 acgcatagcc tgaaatagtt ttgccgtctg ttggttgttt ttcattttct ttctctactt
    10441 tgggagaatc ggctgttagc tcagcttctt ctgtcagccg tttttccttt tcttcttcat
    10501 tattcaactg gtgtaactcc ttcctgttct tttaattgat atttctccaa cgtatctaaa
    10561 tacgtaaagt tcaaacttgc tagtaaacga tcgccaccat ccatgggggg tagccccatt
    10621 ttcgaccgcc cctcattaat cgtgagaagt gaccctttga cctgatccaa tacatttttt
    10681 acttttgttt caggatcagt ctctagtaac tgatcggaat taaagcggat attggtttca
    10741 agtttcgtat ctaattcact cacaaacaca ttaaagtaat gaatcagcgt actttggata
    10801 tactggagat tactttgaac ggtgctagaa tgttcatttt caacgcctaa acgctcaatc
    10861 ggtacaccaa acgccttggc aatttgtttt gtgttccaat cattggaatt gataagcttc
    10921 aaaacatcgg tgtttatttc taatgttttg tagtccattg tttcatcaag aatgatcgtt
    10981 ctaagagcat tatctccact acttgaaccg ttggcttctt caaacttttc acgaattgcg
    11041 ttctttgctg atccgtctaa atctgactta tgaaccttca aaatacctga cccactgaca
    11101 ccacgagtaa agaaattgtg caatgtgcgg ttgccagctt gttgaatttt cagttcatca
    11161 cgtaacgcat aaagtggtgg taatcctgtt aaaccatctt gggtgaaata tttgaagtgc
    11221 aaaatatcgc tagacttcac acgtcttttt ttatcaccaa tctgataaga caacgtacca
    11281 ttatctaatt gagtaaccgt tacacttgag ttaggtaata agtgaatttc ttctaccttt
    11341 tcaccgttgc gtttaatctc tgcaaagcta ttaccgttta gcaacatatt gaccgccaaa
    11401 gcaaacttta aatgccaacc atccatttct gaattaggtt tctcgtttag taacttcacc
    11461 agttcattat cagcttgtgg catattgttt ttgaccaatt gaatcggact tgaagcaata
    11521 tcactagcaa taattcgaac ggctgcaaac acatcactat ttcttaatgc acgaacgctt
    11581 gtaaagctgg tggtgtaatc atccgattgg attgatacca catgttgtag gaaaggatct
    11641 cccgttgtat tttgtctcgc tttaaaaaat gccatctttt aacctccctt ctgttctgta
    11701 tttagcatga ccgctacccc aataagcact atgcccagcg ctaacaagcc atagtagaca
    11761 tttgttagta aggtgatagc aaccaacaca cacaccaatc caagaagtaa aagaacggta
    11821 tgaatgtaat tagaaagaaa attcttcact tgtataaaat tcattgtctg ctttcgctcc
    11881 ttccgttgtg gtgaagtaat ccattgcaaa cacataggca ttgattaatg cagctattgg
    11941 atcaattttg ttactgttct ttgatttatt aatctgaatg ccgttgttat ccgtttttaa
    12001 aatagcgttg ttcactgcat gggtgagaat cgtattttta ttgtgaataa cgttgccttc
    12061 ataaacttgt tctctaaaag ttcgggtcgg aacgtttaga gtaatcgttc cttgtcttac
    12121 ttctaacatt gggtagttgg ctttttcagc tttactgata agcgaattag cgttatacgg
    12181 gtcataacaa atccccatac attccaaatc attttcttga atcaaatctt gaataaactg
    12241 aaatacttgg tcatagtcca cgattccact ttctaattga gtaatggaac actcaccagc
    12301 tttttctagt tctcgataag gcaaaccgtc gcgcttttct ttgtcttgaa gcccatattt
    12361 ggtggctaca aagctatgtg aatcacaata aagttcgccg ttatctagcg gtacgatcca
    12421 tgaaacgcta gttaaatcat ctgttttaga taaatccact ccgatataaa cgggcttgcc
    12481 tgtaatatct tgtggttcta cttcggttgc ctgccagtct tcagcaatca tatagctgtc
    12541 ctcactcgct tgtctccata aattgaagtt tttcaccaat acagaattaa ggttattttg
    12601 tttcaaagca aggttcacat catcttgaat ggttggaatc attacttttt tgatttcttc
    12661 actttcaaaa atcggattgg ctttgatcca tttctcttga tcgtgaattt cttcgggatc
    12721 gtccaattcc catatcgcta taaagtacct gtcggcttgt tcttcgcctt ttagcacacg
    12781 atctaacaat aagtattcct cgtacatagg aacgtttaaa tcaagcccag cggtactaat
    12841 aacacacaat aagccgttct tttgttgggt cattcctgac ttaatgacgt tgtacgtttt
    12901 acgagttttt gcttcgtgcc actcgtcaag aattgcggtt gttggtgcaa acccatctag
    12961 cgtgctggtt tcactagcca gtgccatagc gaacgaatta gagggcaagt gctgaatctt
    13021 agagttcata attttcagtt gtggtcttaa aaacttacta gacttgacca cgtttctaag
    13081 tgaattagac agcatatcat agcccaattt agcttgtttc aaagcgttac ttacaaacaa
    13141 tacttgcctt gcttctgctg gttcttgttc cataatcaac gcattggcag ccatgccact
    13201 tgcgatatac gttttcccat tcttacggct catactggta aacgctcgat tgaatcgcct
    13261 gtaattccct tctttagtac gccagccata aagactgcct agaatgaatt tttgaaatcc
    13321 taacatcttg attgctttac cgtccgtaga tggtagcaac tcaatgaatt taatcgcttt
    13381 ggtggcttgt tcttcatcga acacataagg gaagctatct gacttagaac gctccaaatc
    13441 gcttatatga cgctcacacg cttggataat tttcttgggt gcaatcagct tacctgatag
    13501 gactttctca atgtaatcat tcatgaattc atcaactctt tgaacgggtc ttcgggttca
    13561 tcatttttaa ccttgttcaa agctagtttt gatcgtgatt ctaaggtcat acctagtgag
    13621 gtggcttgtt ttttcaaatc actcatagct tgtgattgaa cccgaaccgc tggattggct
    13681 ttctttactc cgttttcgca tgtgataaac gtacctgatt ttcttatctc ggcttcggct
    13741 gttttcattc gagcaaaggc taaacaatag gctaccaatg aaccataatc tgcttcactc
    13801 aatggataat ctttttttaa tgttggtacg atacgattcc attcacttct agcgctcgct
    13861 gccatccagt cagggggcgt gatatttatt aatggttgct gcttgaacaa ttcttctcta
    13921 gcgtccacac ggattgcttt ttcttcactt gatatattgc cttttgtatc gtgtaatatt
    13981 ttagggtgtc ccatgttgtc ctcctttcag acaaaagaaa aaggaacgta agcaattaag
    14041 ctcacattcc ttcgtttttc gatcagaatt tttctttatc taatttatct atataaatga
    14101 tttttccgtt ctgcatttga atggtcactt gaccaaaatg aggtaactca atggtgcgct
    14161 gtttccctgt tttatcagta ataacaattg tatttttcaa taaatctcct actttcatta
    14221 tatacttttt cacttgtgat tacaagtgta atcgttattt gtaattaccc ttatgccttt
    14281 tcacaacggt tatttgtacc aaaatgagta ggcatcgatt gtcagtaagt ttcacactat
    14341 gcccccttaa ctcaaagggt actcttttct cgtctttgtc tttcattggc tgtcttggaa
    14401 ttatggtgtg catggcataa agaacgaaaa ttagtttcat ctaaccgtct attccaatca
    14461 tcttttagtt caattttgtg atctacaaca tcagctttcc ttatcaagcc ttcttctagg
    14521 caatcctcac atactggatg atttaaccga tacaagtatg aagcctttcg ccatgttcta
    14581 ctttgataga attgaaaata tttaccttcg ctttgctttc tttcttcacg cttaattgcc
    14641 gtttgctttg ctttatgctt cccacaatac ttctgcctat aatcaatcaa tgttttacat
    14701 ccagcatgat tacattgttt tttaactgcc atagattacc aaccaaagcg atctcgttcg
    14761 tcagaatcat cttgttccca ttttgatttg tattcaatat aaggcgaaac tatcttcaag
    14821 cacgttccgt ctttaaaatt aactttagtg attttatctt cctcaaaact aataaagtct
    14881 acatcttctt gagtgtttga atactcaatg tgtttctcac catcgaagta tctaatactt
    14941 tccatatgca taattattct acctccaata aattagttat caattcatca tctgttagta
    15001 gcaaagccaa gaacttatca tggatgttta aaaacaaatt atcctcattc gatgtaagct
    15061 ctcgttgtct cgctcgatca tataggttaa gcagacaatt aaattcatcc attagctcac
    15121 ctgtatcatc atagttgcca ctatctacca atgatcccac tactagcgat tgacaccacg
    15181 ctactgactc ctgcagccaa tcaggttctt cttccattat ctttaatact tctggatcaa
    15241 atgtggaata atcttctgtt accaatccca cactaaatgc cagcgtgttc attagaaacc
    15301 gatccatttc atctgtatat tgaaagttcg gtgctttgtt tgttgaaata atattgagca
    15361 tttactttcc tccttgattc gtaacacgtg gtaacaccta cgtaacaggt acccgttacc
    15421 actcaatccc aacggggaca agggttctag cgttttggta acaggtaacg ggtaaaatcc
    15481 agtaacatta ttccccagca tattagctaa ctatttcaaa agttttaagg aaataaggtg
    15541 ttaccccgtt accaaattgc tgtatccctt ggggcagtaa gggtaaacta gtaacgggct
    15601 aggtgttact gacccgttac tacccgttac tttttgtact ttccagctcg atttttattt
    15661 tcataggcat ttgacttatt gtaaaatcct cgtcgtttat ttctttttgg cagttgcttc
    15721 cgttcccaac caggaatact gctaataatc aaggcaatct ttttagtttc tgcatctgat
    15781 cgaccattca ataaatcctt tgcttgttta tcaaaaacag cttccaaaat atcagcggtg
    15841 agtacgctat caactaaata aaactcttgt tgcatagtag ctccattata gaaatgctgt
    15901 aaatttttct cattacattt actgtcagga tagttctgat aataagaacg tctcgcccac
    15961 gctggagctt cttcccattt atacgggatt tccatttcca aatatttagt gattgcttct
    16021 ttaattggat tctccatctt ggcatcctct tgatagtctt ttgcaatttc taagacttct
    16081 ttatcttcat cgggactaaa atagattcgt tgtcctttat caaaaagcac ctttgcttca
    16141 gctaacactt gaagaaaata ttcatccttt gttttaaacg gattcatctt tggcttattc
    16201 ttacaaggca aaggataaaa acggcggttt cctgtatcat ctttcaaata ctgcccatca
    16261 ttcgacgttc caataaatac acattgcctt gcccaagcta tgacattccg accataagga
    16321 ggtctaattt tatcgatctt ggcactgatg aagtttttta tctgttcaat tttagttttg
    16381 ttcattgaag ataattctcc aagttcaata attacattgc cttgaagctg catataatca
    16441 tctttctttt cacctagcga atctaatgaa tcggtgaaaa aatcatccgt gtaaagtaat
    16501 gctggaacgg tactcttacc aatcccttgc ggtccatcca aaactggaac gatctcaaac
    16561 tttattcctg gtttatagat acgtgcgact gatccgacta tccaacgttt tgttacttct
    16621 cgtgtgtaaa ggttgtcgtc tacccctaaa aggtcaataa acagtgtttc cagtctaggc
    16681 tgttgatccc atttaacttt ttcgatacgt tcttttactg ggttgaaaac attctttcta
    16741 gccacattta ctactgcttt gtgtattttg ttgtcatcaa aaactacatt gtagttttct
    16801 tcaatatatg ccgttaaggc atcctcaaaa ctgtcttccc attctccagc ggtacgattg
    16861 atccaaggag aatcttttaa taggtgaatg tatccgctaa attcgttata agcaattgtt
    16921 ccagctaaat tgctatcatt ggttaaaatc aattctgcat tcacaacact gttactttta
    16981 atctgatcgt ctttattgca tcttaatcgt gacatccagg ttttcaattc aatgatattg
    17041 tcttcacgct cgctggcttc gggaaagtct aaaatactat ctaatttatg cttatgagcc
    17101 atgagctttc cccctcaaat cttttttaat catcgatcga ataatttgat tcacttcttt
    17161 atcttttagc ggtggttcat tgtacgcatt tgctgtataa atcaattctg ctaaagtttg
    17221 attatctaca ccgtgccata gtaaccaacc gcataactta gttaaatgat cgtttctact
    17281 accttcgcca gcgccttgaa caatcatatc taattttttt gctgtccctg tttttgctac
    17341 gggtcggtat tgagtcttta tttcttgcgg tttttttagc agtaggttca ctagccaatc
    17401 gggagctggt gcaatattat ttagagatac gttttcaata ggttcatatg ctctcccaac
    17461 acttcttgaa ataatcatag ttggcgctat tggaactcct ttagtgataa tatcaatccc
    17521 gcttaaactt ttacctttat catcacgccc gaagatatca gcaaatgcac ctgtttcttg
    17581 ttggataatt acaccatcag gaattttaaa gaagaaatgg attcctttac ctggtgtgat
    17641 ttccgtataa gtagaaggaa atggatcata cattgaagca agttttttat aattctcaac
    17701 gccgtttcgt gtttcagaat gttgatctag gtcaactacc accatttgat gatcttcaag
    17761 cattaaccca atatttctag ttgcatcttc cgtccaccat tcattaattt gattagaatc
    17821 gttagttgca tcattccaac cactagagcc ttttattgga attttagttc caggagataa
    17881 gggataaatt gaccatcccg acttttgtaa ttccgttgct gcttgataat tatttgccaa
    17941 taaacctctc ctcctttttc aaaatactgg tataaatcat tcagacacct ccatgagctt
    18001 caactgctca agttcgtatt tcaacccatc ccgaacaaat tcagcacctt ttaattgatt
    18061 ctgatacttt acccatgtat taaatgtttt cacccttgct tcttgcatat cagtaatgtt
    18121 taaccatgcg gttaaaatct gattttttag ctcgttgatt tctcgacaaa ttattttgtg
    18181 ttctgcatgt tttttgagta catacaaaac tgttagtttt tcattgaatg aatttccaaa
    18241 gtaggttttc ccgatattat tttctagttc agcgatccct tgattaattt cctctaattc
    18301 aaggctatcg agttggttta atagttttgt tttgtacatc tacttatgcc ctccagttct
    18361 taattgatag tcgtactcgt cataagcagt gaagagatat aacgctaatc ctgcaaagat
    18421 agctattttt gcaattaatg gtaataagcc gaaaattccg ccgattaaaa tgcttcctat
    18481 aattaagttt cgtttcataa tggttccctc cgatttttta atatggtata atcggaacaa
    18541 agaatatcgg gttttatccc aattaagttg cctttatcct gtccgccaag actgttatgg
    18601 ataaggcttt ttctttgttc tcgatcatgt taatgctcct aagttgtctg caattcagca
    18661 gaacgattat aaattttctc actcactgaa aactgcaacc catatttttc tttaacagtc
    18721 attaattcta ctgtctcatc taaaataggt tcacgatcaa ccaacatttc aggagtcata
    18781 tctttctttt ttaccatttt ggaatgacca taaatagttg atacagcttt attggctatc
    18841 acattcgctt taatgtagtc tactggtttt ggttcagtaa tagcgtggct taatttagtc
    18901 attgcttctt tttggtgttc tttgtctagc atgcggaata cttgaaagcc ttcaagtcct
    18961 gtggactggc gtagttcttt gatgatgtcg aaaatccagt cttgaaatgc ctcggcttct
    19021 tttttacggc ttttgaatgc catgcggtaa atattttttt tcgttgatga tgtttacatc
    19081 ttgtgttctg cccaatgaat cgatgacctt actagtagta accccatctt taggcaaaga
    19141 gtgaatggct ctagtaacct gtttaagacc taacgcttcc gcaacatctt tcgccactgc
    19201 ccaccattca tcattaatat cgacaaatct tataatgtgt ccgttccaaa tttcagtttt
    19261 cattcaacgt cctccagttc aagccctagc aagctcgcta acaactcaac ttgctgatac
    19321 aaaatttctt gatactcttc aatagaaagt cgttctttaa actctgcttg tactaaatcg
    19381 ttcaagccac gcaatactcc gcttaccgtt ggttcttgtg gtagcaattt ttgatagtcc
    19441 atttgttcgt acaaacattc aaacgtttct tctagcgctt tgccttccaa gtttgctaat
    19501 tctgccgctg tgtcttctgt aagaattgtt gataatactt tttcgattgc tttattttgt
    19561 gtcgattcca ttattatttc ctcctattaa tccctttgcc cccacggctt gcgtttattt
    19621 ttggtattct tccataaagc gatcacattc atcttgtgaa attcttacga ctccatcaac
    19681 tataatcaac tttaaaccgt taggaacata tttctgcatc atagtattgt atgaaacgtt
    19741 catatactta gcagcttgtg ccttgttcat atattttgga aactggtact ttttttctaa
    19801 taatgaaagt aacgttcctt gagtttgctc tacttgttga acaactcgat cgtcaataat
    19861 tttaaaagct ggatcatcaa gcgtgagttt gtcaattttt aattccatat ttatcccccc
    19921 tcgttagcat acatttctat acaaagattt taaaaaaatg caagaggctc ttgaatacat
    19981 ttctatacct cttataaact caatatacca tcataccttt tcatttgcaa gaattttaac
    20041 atactttttt gaacttttat gtttcacaca tatttaaatc gtgatatagt agagaaaata
    20101 tatgaatggt ggtgaaaatg tggctagaaa aaaatacccc gaagcagtgg agacaggaca
    20161 aagaattaga ctggctagaa ttgaaaaggg actctcaatg gaacaacttg gaggtaaatt
    20221 atccccaccg gccagtaaag gtgccgttag taattgggaa aatggctata atcttcccaa
    20281 taacaccagg cttaaacaac tttcaaaggt attagatgta tccactacct atctattaaa
    20341 cggatcttat acattaagag atatgcatat gatgcccgaa attaagcgaa atgtgcttga
    20401 aaagcaattg aaaaaaacta ttgaaaaaaa taactctata attgattcta atctttctaa
    20461 tgctatcaat aacatcgaag aagataacct gactgtctca cagaagattt atttaacaaa
    20521 cgcaataaaa tatcttaaaa caggatctcg aattgaattt cctgacggga gtaccccaat
    20581 tgcttatctc gcagctcttc taaatacgct tttacgatat gactcattaa ttaatgattc
    20641 agaaaaaaat acagatgaga aaaaagagta tcaagaattt tatgaaaatg atcttctaaa
    20701 agagttcaca gctcttgtta ctgagctaaa taaacactat tcaaaataat aataaactgc
    20761 ctcctccaac cgcccccacg gcttgctaca ctatggaggg aaaactatta tggcaacatt
    20821 caagcaatat gaaactaaaa aaggtaaatt ttggctctat gaagcctatc ttggaatgaa
    20881 caaaatgact ggcaaacctg ataaggttcg tagacgaggt tacaaaacaa aaaaagaagc
    20941 ccagcttgcc ttgtcacgct tgcaagtgga ctacgataaa aacggtatta aaaaagcgaa
    21001 caacgaaacg tttaaagaag tttatgactt gtggctagaa acatataaaa ccactgtccg
    21061 agaagccacc ttcatgaaaa ctgaaatcaa gtttagaaag tggattcttc caaaatatgg
    21121 aaatcttcgt gtgaatgaag tcacggtaaa aaaagcccaa caaattgtaa atagctgggc
    21181 tgaaactact gaccaataca aggtacttca ttcaacttct aagcgtattt ttagctacgc
    21241 tatcaatctt gggattattg agactaatcc actagacaac gtattaatgc ctaaaagaac
    21301 caccactaag cataaagaaa aggtcaaagt ttacagtaaa gaacaactga tcaccttgtt
    21361 tgattaccta aacagcaaac ctgaaactta tcgaaacgac tttgataaaa cgttactacg
    21421 ctttctcttt tatagcgggg ttaggatcag tgaagccctt gcattgaatt ggtcagacat
    21481 tgattttcaa gaaaatacgg tcaccatcaa taaaacacta agccaatcca aaaatggcta
    21541 taaaatcagt gatcctaaaa ctgattgtag tgctggggtt cttccactag atgaaagaac
    21601 aatttctata ttaaaaaaat ggcagattaa tcagcggaaa tatatgctga cacttggtgt
    21661 aactgatccg actatgattt tttgtggtat ctataaagaa ctaatcacac accacgctat
    21721 ttatgcaaga ttgattacta tttgtgaaaa agctatggtt ccatttctcg gtgtgcatgt
    21781 agcaagacac actcatgctt caatgctctt ggacgctgga gctactatgg tagaagttca
    21841 aactagatta cggcatacaa aaattagtca gacgatcgat tgttacggtc acttggcaaa
    21901 agaaacgaaa gaaaagactg ttgaaaaatt agtacaacac ttaaatattg actagatggc
    21961 atacaatatg gcatacagta aaaaataaaa aaaggagaaa ccctatcata tcaaggtttc
    22021 tcctagcttt atagtctatt cttcttcttc cgccatgaat gtattgaata cttcttcaat
    22081 catgtcccat tctgcgtctg tttcgatttg ttctaactcg ccttctgttc catcttcgtt
    22141 ttcgatgtat gcataagctt gtaattctac atcttcatct tctgaaacac cagcaggata
    22201 taaaagaaca tagttacgtc caaattcttc ttgtccgtca actgttagta ggatttcata
    22261 taatgtttca tttccttggt catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg
    22321 ggaatggtta tgttctgtca taagattacc tcttttcttt tattattaaa ataattgctt
    22381 acctgagccg tctaaataat tttgtaagat catcacagct gctaatttat cgatgacttt
    22441 ttttcgtttg gctcttgaag tatttgcctg ctctactagc atacgttctg cctgaacagt
    22501 ggttaatcgt tcatcttgat aatcaactgg caaggagaat agttctttga tcttttctcc
    22561 gtatgacatg gaagcttctg cccttggtcc gatggaattg ttcatatttt taggtaatcc
    22621 aactacaaac cggtctactt catattgtgc tactaattcc ttcaaccgat caaagccaaa
    22681 ctcttcttct gcttcattga tccgaatgat ctctactcct tgagctgtcc agccaaaggg
    22741 gtcactgata gcaacaccta ctgtcttaga accgacatcc aagcccatta ctctcataaa
    22801 ttcactccat gatttgagag gtagtccttg acaattactt ccatgatctc atcgcgttcg
    22861 tgacggcgaa tcaaatttcg agcat
//
LOCUS       DAYLZO010000036        22771 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.036, whole genome shotgun sequence.
ACCESSION   DAYLZO010000036 DAYLZO010000000
VERSION     DAYLZO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.036"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..132)
                     /locus_tag="ACGVJG_001262"
     CDS             complement(<1..132)
                     /locus_tag="ACGVJG_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFJ6557231.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     "
     gene            complement(387..1112)
                     /locus_tag="ACGVJG_001263"
     CDS             complement(387..1112)
                     /locus_tag="ACGVJG_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFJ6557232.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(1473..1543)
                     /locus_tag="ACGVJG_001264"
     tRNA            complement(1473..1543)
                     /locus_tag="ACGVJG_001264"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1509..1511),aa:Gly,seq:ccc)
     gene            1660..2412
                     /locus_tag="ACGVJG_001265"
     CDS             1660..2412
                     /locus_tag="ACGVJG_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFJ6557233.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            2428..2907
                     /locus_tag="ACGVJG_001266"
     CDS             2428..2907
                     /locus_tag="ACGVJG_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFJ6557234.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3158..4738)
                     /locus_tag="ACGVJG_001267"
     CDS             complement(3158..4738)
                     /locus_tag="ACGVJG_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFJ6557235.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(4731..5474)
                     /locus_tag="ACGVJG_001268"
     CDS             complement(4731..5474)
                     /locus_tag="ACGVJG_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557236.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(5650..6426)
                     /locus_tag="ACGVJG_001269"
     CDS             complement(5650..6426)
                     /locus_tag="ACGVJG_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFJ6557237.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(6674..7510)
                     /locus_tag="ACGVJG_001270"
     CDS             complement(6674..7510)
                     /locus_tag="ACGVJG_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFJ6557238.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(7521..8450)
                     /locus_tag="ACGVJG_001271"
     CDS             complement(7521..8450)
                     /locus_tag="ACGVJG_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFJ6557239.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            8642..8995
                     /locus_tag="ACGVJG_001272"
     CDS             8642..8995
                     /locus_tag="ACGVJG_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFJ6557240.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9069..9725)
                     /locus_tag="ACGVJG_001273"
     CDS             complement(9069..9725)
                     /locus_tag="ACGVJG_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFJ6557241.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            9951..11885
                     /locus_tag="ACGVJG_001274"
     CDS             9951..11885
                     /locus_tag="ACGVJG_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFJ6557242.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(11971..13194)
                     /locus_tag="ACGVJG_001275"
     CDS             complement(11971..13194)
                     /locus_tag="ACGVJG_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFJ6557243.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(13188..15059)
                     /locus_tag="ACGVJG_001276"
     CDS             complement(13188..15059)
                     /locus_tag="ACGVJG_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFJ6557244.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(15080..16198)
                     /locus_tag="ACGVJG_001277"
     CDS             complement(15080..16198)
                     /locus_tag="ACGVJG_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFJ6557245.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(16281..17114)
                     /locus_tag="ACGVJG_001278"
     CDS             complement(16281..17114)
                     /locus_tag="ACGVJG_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6557246.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(17107..18024)
                     /locus_tag="ACGVJG_001279"
     CDS             complement(17107..18024)
                     /locus_tag="ACGVJG_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6557247.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(18047..18226)
                     /locus_tag="ACGVJG_001280"
     CDS             complement(18047..18226)
                     /locus_tag="ACGVJG_001280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557248.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(18251..19375)
                     /locus_tag="ACGVJG_001281"
     CDS             complement(18251..19375)
                     /locus_tag="ACGVJG_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFJ6557249.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            19558..20466
                     /locus_tag="ACGVJG_001282"
     CDS             19558..20466
                     /locus_tag="ACGVJG_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6557250.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            20607..22220
                     /locus_tag="ACGVJG_001283"
     CDS             20607..22220
                     /locus_tag="ACGVJG_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFJ6557251.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7154 a         4421 c         3799 g         7397 t
ORIGIN      
        1 gccgacaatc tgattgattg gattgtagcc tttttcttcc aaagcccgat aaacaatcgc
       61 taatgtttcg ctcacttctt ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc
      121 tgtaaaaccc atgattctac acctctttcc cgcaaggtaa ttccagctta ccttctacaa
      181 ccttatttta ctcgaagaaa gggaaaacta caaacaaata ttccaaactt ccgaataaat
      241 taagaaacac tatttgtatg tttttttcct tttttctgta gctttatcaa aagtactctt
      301 ttcataaagt atacgctata tttctttgaa tgtatgtttt tacacaattt tttcataata
      361 ttcataaaaa attcgaatag ctgctactaa ttttttgttt ttgttgcttg tcctgtctca
      421 taattgatcg tgtagccatc ttgaacattg tagataaaga cattgaattc aatttttttg
      481 tcttcaatgc tttctgcttc caattgcact cccctggcta ccagttcatt cccctcaaaa
      541 tgaggagtga cacggtagcg tacatgatga tttgtttctt tgatatatgc cgcaatatca
      601 ttttcgtgag caagcatgta tggagcattc aacgaacgtg tccctgtcat caagttttta
      661 atgttgttat tttgaccagt cagctggaaa ccaattaaat ggctgcgatt atataaccat
      721 tgcccgtcac ttaatttctt ttgtttccaa cctgtcgggt caatatacaa aggctctctc
      781 ttttcagtag ggaacaaatc ttttccaagc atagcattgg cttgtcctac tctgttcaat
      841 ttatcaatat ttgaatagct ttcccatgtt cctttggaaa ggctaagatc ctcttttgta
      901 aattccggct gattcccatt gatttctatt tcttgatgtt ttccatcata ttctggcaca
      961 tccgctactt gagaagcttg tggtgtggaa tcaaaaatag ttgtaatctg ctgaaagata
     1021 tctgaatctt tttgtgtggt ggaaacaccg aatgcccctg caatcacagc agcgacgatc
     1081 cccacgatcg ccttggttgt ttttttgctc atttctttac tcctttacta acagctccct
     1141 aaatcttaac atatgttccc ctataaataa atactagaaa gaaatagaaa cagcaaaaaa
     1201 taaaacctgt tttaagatgg cttaaaaata caaaaaagag acggattcct cgtctctaat
     1261 cagtcaccac tctatctgta aggtatttat tattattcaa aaattttctt catactcaag
     1321 acaatacact gatttcacaa ccgcttggta aacggtgcgc aaaagcttgc atctgcggta
     1381 ataagtccat ccaagttaaa aacataagaa gttgtttttg cttggctgtt cttattactt
     1441 gttgcagagc agctttttca caacctattt taagcgggtg acgggaatcg aacccgcgac
     1501 atcagcttgg gaagctggga ttttaccact aaactacacc cgcaatacaa aaacagtata
     1561 cgcctctttt attccttcct caagtttttt tattctttac gcttctggta tgttttgtag
     1621 taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa agaattagat
     1681 ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa tgcggagatg
     1741 tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt ccgtttggtc
     1801 agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac catccgaatg
     1861 gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa tgatctttca
     1921 cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact gcaagcaatc
     1981 gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc agtgatcagt
     2041 ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac tttagtcatt
     2101 ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat caaattttta
     2161 tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt acgttttggc
     2221 ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt tcctggagtt
     2281 caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg agtttctcgc
     2341 ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt tattttccag
     2401 cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt ttcttttagt
     2461 tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc attgatttgt
     2521 tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca aataggcgga
     2581 tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag cgatcttttc
     2641 tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt tcctctagtc
     2701 cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta tcgagaagcg
     2761 gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg tcttgtatta
     2821 tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc aatcatgatg
     2881 aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt tgtgaaagag
     2941 acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt aaccgcagga
     3001 gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt ctcacatttt
     3061 tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat ctgtaaaaaa
     3121 ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt ttccaaaacg
     3181 ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg atcacaggaa
     3241 tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg ataagaataa
     3301 tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct gaacctctgt
     3361 taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct ctagcgaaat
     3421 aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta gggataaaaa
     3481 acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt aaaaaactct
     3541 gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag attggcaaag
     3601 aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata aaagaagtct
     3661 gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg attaacttat
     3721 tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata aaaatgattg
     3781 gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt actaattgag
     3841 cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt ctttttattt
     3901 tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc agctgtttta
     3961 cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat aaaccttgtt
     4021 gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc ggatgccgat
     4081 ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt ccagcaaatg
     4141 cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt tttttaaaca
     4201 ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga ataatcaaaa
     4261 atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga tcagctcgcc
     4321 atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg gtcaaagcaa
     4381 ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc aaaaatactt
     4441 gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga gttaaaccta
     4501 acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca aagttcatag
     4561 caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga ttttgagaca
     4621 aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta ttcgtgagct
     4681 gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc ttatacatac
     4741 ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc tagcgtttca
     4801 tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc acctttcttc
     4861 aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt agaaaaaatg
     4921 actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa tgctgcttga
     4981 ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg cagcaacgtg
     5041 ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt ttcattttga
     5101 tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt tttttgtgtg
     5161 tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa ttgtaaaaaa
     5221 atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg attttccgtc
     5281 aatttcattc catctactaa aatcgttccc gatgatggtt caataatact aactaaactt
     5341 ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat ttccccttgt
     5401 cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt acttacattg
     5461 atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg tacccttcgt
     5521 tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa cgactaggct
     5581 gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag atatgagtct
     5641 ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct ttagtttatc
     5701 aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta tttcgtttac
     5761 tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat ctttacataa
     5821 atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga gaatctcttc
     5881 atctttcaat actaggccta ttttattttt ataacctggc atgctcatta gataaaggat
     5941 ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc accttttttt
     6001 cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt ggatgctttt
     6061 tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa tctttcgatt
     6121 aataacagtt attgctttat tcggatgaga cgttagataa acaattttac acttttcatt
     6181 cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca aatcgatgtc
     6241 taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa caaattgtat
     6301 aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc tggattcttc
     6361 caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa atatacgcgt
     6421 taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat acaacttttc
     6481 aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac acctttgaaa
     6541 ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa atgaatacat
     6601 catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag ctatgtttat
     6661 ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca gagactttct
     6721 cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa tagtctgatt
     6781 ggatggttcc ttctatttta taccattcat catttttaac cgttactcct tccggcatat
     6841 gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg aatcggaaca
     6901 agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca tagctgattt
     6961 ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct aaatagtttt
     7021 catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca ttcatttggt
     7081 ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat tgtgtctgca
     7141 tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg gcttctacga
     7201 tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc aaagctagca
     7261 gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga tcgtgctcgt
     7321 gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact aaagccaaaa
     7381 gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg atatattgat
     7441 cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat aaaatcaaaa
     7501 accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct aagggcaatc
     7561 agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat cagtagattt
     7621 ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt gccgaacaga
     7681 cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat cgtaaaggcc
     7741 aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag cataattcta
     7801 gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa ataacgacct
     7861 gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg gatcagctga
     7921 ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg gtgattttct
     7981 gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata ggcaagccag
     8041 acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc catctgccaa
     8101 gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat cggagcagtc
     8161 agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg atgaacgatc
     8221 ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc agtaaaaata
     8281 gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa aaatacttgt
     8341 aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat aacgatcgat
     8401 aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat gagtttcctc
     8461 ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa aaaacaagtt
     8521 gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt gactttttct
     8581 gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag ggagaataaa
     8641 aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt catccgttgc
     8701 cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc cacgactaga
     8761 ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg aaacaccaga
     8821 agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc atcaaggaaa
     8881 agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc tactagatga
     8941 actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa aataatcgtt
     9001 gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg ttcaatcgct
     9061 ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg caagaaatag
     9121 atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc tgacggagca
     9181 ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt taactgattg
     9241 actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat ttgttggatc
     9301 ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac gatgcgccca
     9361 acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact ttgatggaat
     9421 ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac atttgtcaat
     9481 tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc ataaccatac
     9541 ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc actatctact
     9601 tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt atttaaaatc
     9661 cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat aatatgatct
     9721 ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca tcatagcagt
     9781 atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt ctttattcat
     9841 ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac tactaattga
     9901 aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc atgatattat
     9961 tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa aacatacatt
    10021 tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct ggaaaatcga
    10081 ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc gctaaaaata
    10141 aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat gaaacagttt
    10201 ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg atgcgcgagc
    10261 tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct tatttaaaag
    10321 aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat gaaaatgaaa
    10381 ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag aaaatcgata
    10441 ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg caacaccctg
    10501 acattttgat tctagatgaa ccaacgaacc atttagatat cgagacactt tcctggcttg
    10561 agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac cgttattttt
    10621 tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc cattacaaag
    10681 gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa tggaaggctt
    10741 atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg aaccttgttc
    10801 gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa atggaccggc
    10861 tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata gaaaagcctt
    10921 ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat gtcaccttat
    10981 ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta ggtccgaatg
    11041 gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt atccgtggaa
    11101 aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa gctgatctgc
    11161 atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact ccagaaaaag
    11221 agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa aaaccaatcc
    11281 ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg atgaacaaag
    11341 aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac aaagaagtat
    11401 tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat gaccgatatt
    11461 tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc aaagtctatc
    11521 taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc gccgaattgt
    11581 tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat tattaccaaa
    11641 gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag gttgaagaag
    11701 aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc gcgccagaaa
    11761 acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct gctagacaag
    11821 atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa gaatatgaaa
    11881 attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct ttttttattt
    11941 tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat tactagtcat
    12001 tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat ttaccccatt
    12061 atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt ccgttgtagc
    12121 atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata agttcttttc
    12181 tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca tgatcaccat
    12241 actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc aataacaaat
    12301 ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta aatcaaaata
    12361 tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc atgcttggcc
    12421 tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc ttggttttcc
    12481 atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta ctaaataatc
    12541 tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc cagtttcttc
    12601 actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc taccttgttg
    12661 tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc aaaaacgctg
    12721 aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc ccgttatttt
    12781 ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat tagtgataat
    12841 ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt gttgaaataa
    12901 aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc cttctgtcca
    12961 accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg ggaattgata
    13021 ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac gctcgatctc
    13081 attattccat tctttttcca ttaattcttt ataacattca tttatccatt tttctggatg
    13141 tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta gcataatttt
    13201 agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc aaaatcaatt
    13261 ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc aaccactcct
    13321 gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat aaaagtgttt
    13381 tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt tcgtttaata
    13441 gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct atagttttcc
    13501 ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc aaaccctgtc
    13561 tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg ccagccttca
    13621 aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt agaggcaagt
    13681 acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg tccataggtt
    13741 ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg aatccctagc
    13801 attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga aaaaccaaat
    13861 tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa atttggccct
    13921 aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag caaattggct
    13981 tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc cgtccaatac
    14041 tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa tggagataac
    14101 gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat attcaggata
    14161 cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt gactaacccc
    14221 ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga ccaaaagctt
    14281 gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc atcttccgca
    14341 aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat ctggctaatg
    14401 ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc aaaaggatta
    14461 taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag ataacaattt
    14521 tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc aaatttttgg
    14581 ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca ttcatattca
    14641 aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa ttttttccat
    14701 aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac catacgctgg
    14761 tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc agcttttatt
    14821 cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg ccccgctccc
    14881 cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc taccccttga
    14941 tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg ttgatttatc
    15001 tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg atgtttcatt
    15061 gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat cgaagtaagc
    15121 cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt tctctatggt
    15181 atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt gcatagtgct
    15241 tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat cgtacagagg
    15301 aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca ctggtatcaa
    15361 tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt tcgattttct
    15421 gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt tttgtgtaac
    15481 gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa caagaattct
    15541 cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca tcaaattctg
    15601 cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta tctgcatgct
    15661 gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa tacagtaatg
    15721 gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc catgctacta
    15781 aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt aaccctagtt
    15841 gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt aagaataaaa
    15901 aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat aattgttctt
    15961 gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc tgccataaaa
    16021 taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt acgtctgtat
    16081 attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat tccgcatcta
    16141 tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt tcattcatgg
    16201 atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac gaactagatg
    16261 gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc cttaaattta
    16321 gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa aattagcata
    16381 attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt gataattaag
    16441 ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata ttgattccaa
    16501 cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat cggtacagcg
    16561 attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc catcagtgct
    16621 gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact ggcaatcgaa
    16681 gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat gtaaaataaa
    16741 ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt gaacaacagt
    16801 ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga actggctgta
    16861 gctaaaactg tactgattac agcaataaca atcgaattcc aaataatcaa gggataatta
    16921 tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt gaaattgatt
    16981 ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata taaatatggc
    17041 atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct gctttgtatc
    17101 ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata atataacaga
    17161 tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca tttcaaaact
    17221 tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat tggaagtccc
    17281 aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta aaactgtggc
    17341 cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc gaaatttcga
    17401 tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa gaccagctaa
    17461 aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca ttgatattcg
    17521 tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat tcaaaaatcc
    17581 attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag ccaccgttga
    17641 tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta actgtgctga
    17701 attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg atcctgccat
    17761 gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga tagctagata
    17821 attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa aagcaaacga
    17881 gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg tgaccaatcc
    17941 ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct ttattgtctt
    18001 gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa tcaccagtta
    18061 attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa ttttcaaaat
    18121 cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca tctgaataag
    18181 tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt tcatagtatt
    18241 tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag gccaatgagt
    18301 tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct gtcagcataa
    18361 atgtaagaaa ttgttgtgcc acttgtggat tttttgcatc tttagggaca taaaaacttg
    18421 atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc agtggagcca
    18481 tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca taccagccgc
    18541 cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca ccgtagaaat
    18601 gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc gcttctttag
    18661 tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg gctagcatgt
    18721 tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta ccagtttttt
    18781 cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct tcaaaatcta
    18841 cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta aaatcactag
    18901 cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat ttttgatcat
    18961 aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa aaagcatctt
    19021 gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata tccggcatat
    19081 cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc gaagctacat
    19141 tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt gcagcatttt
    19201 caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg gcttttttta
    19261 tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat gcacttaagc
    19321 tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt ttcatgtcta
    19381 tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata ttaacggaat
    19441 tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct tttcattttt
    19501 gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga aaggaaagtg
    19561 gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact aacctattcc
    19621 atagataccc atcttcattt atctgctgct ccctattttc gactactaat aaatgaaagc
    19681 gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc ttccctttta
    19741 ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac acaaactttt
    19801 atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg acaatctttt
    19861 tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca gttcaaaatt
    19921 gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa ttcttttttc
    19981 atactatgcc aacagatgag cattcatttg ctttccttat tagttactta ttgtctgcaa
    20041 aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt attgacaata
    20101 ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga tgaattagtt
    20161 gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct tgaacaatta
    20221 gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa agtgaatgga
    20281 aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg tctcgtgcta
    20341 gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc gatccatcat
    20401 ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta tctatcctta
    20461 aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt tctgtctatt
    20521 caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata acatagtacg
    20581 atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt gtagctggcg
    20641 gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc attatctatg
    20701 aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta ggaggtgtga
    20761 tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca aaaaacaaca
    20821 tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag cgattaacag
    20881 ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg atcatctctt
    20941 ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa aacggctttg
    21001 tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa aaaaatccac
    21061 aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag aatttcaagc
    21121 atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc ccttatgatc
    21181 cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc gttcatttag
    21241 aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca gatggctccg
    21301 tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat gaagtggtga
    21361 aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac cagttacaaa
    21421 ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt gtagtgactg
    21481 gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat atgctggcag
    21541 atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt gtcgcaaaaa
    21601 tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa gcatcaggca
    21661 taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc tatccagaag
    21721 ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt ttaggtggac
    21781 aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta gcaatcaccg
    21841 gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca ccgtattcta
    21901 ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg acgaatatcg
    21961 tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg ttctttttag
    22021 atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa aatattccaa
    22081 tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt tacattactt
    22141 tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg ggtatcaaag
    22201 cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct aggagttatt
    22261 tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat gtatatagaa
    22321 tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg ttatagttta
    22381 agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat aaaaaaacat
    22441 gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac tacgcctaat
    22501 attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc cctttcgaat
    22561 attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa tcgactggct
    22621 tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg taatcatatt
    22681 tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt gtgtattaaa
    22741 taatttaatt tttttgttcc tatttattta a
//
LOCUS       DAYLZO010000037        22524 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.038, whole genome shotgun sequence.
ACCESSION   DAYLZO010000037 DAYLZO010000000
VERSION     DAYLZO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.038"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            142..315
                     /locus_tag="ACGVJG_001284"
     CDS             142..315
                     /locus_tag="ACGVJG_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557252.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            955..1428
                     /locus_tag="ACGVJG_001285"
     CDS             955..1428
                     /locus_tag="ACGVJG_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFJ6557253.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1437..1664
                     /locus_tag="ACGVJG_001286"
     CDS             1437..1664
                     /locus_tag="ACGVJG_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557254.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2300..2671
                     /locus_tag="ACGVJG_001287"
     CDS             2300..2671
                     /locus_tag="ACGVJG_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557255.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2927..3169)
                     /locus_tag="ACGVJG_001288"
     CDS             complement(2927..3169)
                     /locus_tag="ACGVJG_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFJ6557256.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3201..4103)
                     /locus_tag="ACGVJG_001289"
     CDS             complement(3201..4103)
                     /locus_tag="ACGVJG_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFJ6557257.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4116..4304)
                     /locus_tag="ACGVJG_001290"
     CDS             complement(4116..4304)
                     /locus_tag="ACGVJG_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFJ6557258.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4318..4881)
                     /gene="amaP"
                     /locus_tag="ACGVJG_001291"
     CDS             complement(4318..4881)
                     /gene="amaP"
                     /locus_tag="ACGVJG_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFJ6557259.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4919..5812)
                     /locus_tag="ACGVJG_001292"
     CDS             complement(4919..5812)
                     /locus_tag="ACGVJG_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFJ6557260.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5890..6813)
                     /locus_tag="ACGVJG_001293"
     CDS             complement(5890..6813)
                     /locus_tag="ACGVJG_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFJ6557261.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6862..7212)
                     /locus_tag="ACGVJG_001294"
     CDS             complement(6862..7212)
                     /locus_tag="ACGVJG_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFJ6557262.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7245..8147)
                     /locus_tag="ACGVJG_001295"
     CDS             complement(7245..8147)
                     /locus_tag="ACGVJG_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFJ6557263.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8140..8997)
                     /locus_tag="ACGVJG_001296"
     CDS             complement(8140..8997)
                     /locus_tag="ACGVJG_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFJ6557264.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9331..10140
                     /locus_tag="ACGVJG_001297"
     CDS             9331..10140
                     /locus_tag="ACGVJG_001297"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFJ6557265.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10180..10677
                     /locus_tag="ACGVJG_001298"
     CDS             10180..10677
                     /locus_tag="ACGVJG_001298"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFJ6557266.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11062..11135)
                     /locus_tag="ACGVJG_001299"
     tRNA            complement(11062..11135)
                     /locus_tag="ACGVJG_001299"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11099..11101),aa:Ile,seq:cat)
     gene            11322..11645
                     /locus_tag="ACGVJG_001300"
     CDS             11322..11645
                     /locus_tag="ACGVJG_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557267.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11809..12063
                     /locus_tag="ACGVJG_001301"
     CDS             11809..12063
                     /locus_tag="ACGVJG_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557268.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12133..12378
                     /locus_tag="ACGVJG_001302"
     CDS             12133..12378
                     /locus_tag="ACGVJG_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6557269.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12454..12819
                     /locus_tag="ACGVJG_001303"
     CDS             12454..12819
                     /locus_tag="ACGVJG_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFJ6557270.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12880..13443)
                     /locus_tag="ACGVJG_001304"
     CDS             complement(12880..13443)
                     /locus_tag="ACGVJG_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557271.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(14011..14211)
                     /locus_tag="ACGVJG_001305"
     CDS             complement(14011..14211)
                     /locus_tag="ACGVJG_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFJ6557272.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            14607..14756
                     /locus_tag="ACGVJG_001306"
     CDS             14607..14756
                     /locus_tag="ACGVJG_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557273.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            complement(14963..15676)
                     /locus_tag="ACGVJG_001307"
     CDS             complement(14963..15676)
                     /locus_tag="ACGVJG_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFJ6557274.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(15669..16754)
                     /gene="uxuA"
                     /locus_tag="ACGVJG_001308"
     CDS             complement(15669..16754)
                     /gene="uxuA"
                     /locus_tag="ACGVJG_001308"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFJ6557275.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(16771..17214)
                     /locus_tag="ACGVJG_001309"
     CDS             complement(16771..17214)
                     /locus_tag="ACGVJG_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFJ6557276.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(17248..17601)
                     /locus_tag="ACGVJG_001310"
     CDS             complement(17248..17601)
                     /locus_tag="ACGVJG_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557277.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(17713..18114)
                     /locus_tag="ACGVJG_001311"
     CDS             complement(17713..18114)
                     /locus_tag="ACGVJG_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557278.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(18151..18660)
                     /locus_tag="ACGVJG_001312"
     CDS             complement(18151..18660)
                     /locus_tag="ACGVJG_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFJ6557279.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(18682..19539)
                     /locus_tag="ACGVJG_001313"
     CDS             complement(18682..19539)
                     /locus_tag="ACGVJG_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFJ6557280.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(19557..20390)
                     /locus_tag="ACGVJG_001314"
     CDS             complement(19557..20390)
                     /locus_tag="ACGVJG_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFJ6557281.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIVGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(20404..21201)
                     /locus_tag="ACGVJG_001315"
     CDS             complement(20404..21201)
                     /locus_tag="ACGVJG_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFJ6557282.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(21234..21518)
                     /locus_tag="ACGVJG_001316"
     CDS             complement(21234..21518)
                     /locus_tag="ACGVJG_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFJ6557283.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(21515..22516)
                     /locus_tag="ACGVJG_001317"
     CDS             complement(21515..22516)
                     /locus_tag="ACGVJG_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFJ6557284.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
BASE COUNT         6546 a         4466 c         3659 g         7853 t
ORIGIN      
        1 taaatttttt cggagaatta aaaaatacaa taaataactt tagttacttt aatgcgttct
       61 tactttattt ttatattatc atttttctta ttcacttctt tggtttatac tattcttata
      121 aataaaaacg aggtatgtcc tatgatgaaa agattgtact attcattgat cataacgatt
      181 ggctatttga ttgtatcaaa tctaggaaat atggtatttg gtatcagtaa agaattcagt
      241 tggactacta cattatggga atctctattc tttttcatct tcgtattttt gttacaaaat
      301 tatcgaaaaa aataaacatg tgccatctat ctgttggcac atgttttagg atttatggta
      361 ctttttattc ggcaatccat tttatttttc tacttcttct taatacctct aacacttttg
      421 atttataaac taatccaatg catctacttg cgcttattaa gctatttcag ctagatagta
      481 ccaccccatg cgtcaatgct tgattttcct tttgtataac attgtataag caataaagga
      541 caaataagtg gtgattacac caaaccattg aataagaaaa attctgatac gtaaataaac
      601 atgaacttga aaaaattaat gaatcggtta gctactacgt agttgtcttt tttctcatac
      661 agttctgtta acttacactt ctcttcgcta gataataaaa aatcctccaa ctgtattgtc
      721 caatataatc ccaggcatct aaactagcat tatagaagag acacacatag aatctaaaat
      781 aactggtttt aaatgtttct tacaatcaaa aaaagctgtt aaatgcactc cacaccttca
      841 tactctatca ttttttataa acgatatatt tttattgttt ttcgataaaa aatagttgtt
      901 ttacaataat tattctgcta tattttagct atcaaagata aaagagggat aaaaatgaaa
      961 tttaaaacat tgctattaaa atttgtcgtt ttaggtataa gtgccgtatt tattttgttc
     1021 ggcgtacttt tttatactca gatcgctaca agcgaaaaag gattcaattt tgattggcag
     1081 accacattat ttgtttctgt catttacttt gcccttctcc tagggatcgt tattgcttat
     1141 ttcttattga aactattgaa aaaaatcgaa gataaccaag ctttttccac tcaaagctta
     1201 aacatactaa aaaaaatcga acgaaccatt ttgtttattg gtttcgcctt ttttggctta
     1261 cttcctaaat ttatgaaagt agcggattta gacgatgctc caggtttgat gttggtagga
     1321 atcgctgtcg tattcattcc ttttgcagta tacacattaa caagcgtgct ggaaaaactg
     1381 cttgaacatg cgattttact taagacagat catgatttga cagtgtgagg tgcgacatgg
     1441 caattatcgt taatttagat gtagtattag ctcaaaaaaa aatgagtgtg actgaattga
     1501 gtgagaaagt gggcattacg atggcaaaca tctctattct aaaaaatgga aaagcaaaag
     1561 ccatccgttt ttccactctc gaaaaaatct gccaagtttt ggagtgtcaa ccaggtgatt
     1621 tgttgaccta taaaaatgac tcgaatacta taccagaaga ataaaataaa gtagaacaaa
     1681 cagtaaataa aaaataatct gaatgataga tgtgtgagac ttttagaagt tatttacgtc
     1741 tattattcag attttttatt tcgattgaat accgttttat ctataaataa gatatgtact
     1801 gactcataca aagggggcct tttcttctcc atgtactatc acttttcctg atacaactgg
     1861 tttcctgtta cgttgtagtt ggatctgctt ttagtataat actttattca ttccaccaga
     1921 ctgatagcag tttgagcagt ctaaataatc atgtagtatt acaggccatt ctgtttcata
     1981 ccttgaagtg cttaaaactt cagctaatgg cccattgtgt taactcttac tccattcttt
     2041 tttagtaagc tcccatatat actgtaaagc cttctatata ttatgtcatg ttgcttttta
     2101 acatttacta tgtagttttg ataaaatgat ttgaataaca gtgatggaca attctttttt
     2161 atttttaaca ttattttgat aataatgatt atttatttaa aaatacatta gataacattt
     2221 gttacgacac gaaaaagctt cacaaacaaa tatcgttatt ttaaaatgag ggcgaactca
     2281 cacaattagg aggaatagta tgccatttat tgtttacttt tttctttcat tactcgtgat
     2341 ctatattcca ttaccaacat ttttcctgct cgtcaatcgt ctagcagagc aacatgatac
     2401 gattgctgct cttacgaaac tttgtttact tctatttgtt tgtctagatt actttacgag
     2461 ctattattgg ctaaaacaga caagagtaaa aaagagatat tatttcttcc ttgtcttttt
     2521 caatctcttt gagtttctga tccattgctt tttacttcat cagtatcata cgatccattt
     2581 gtggatcatc tgggttttca aagtggtcgt attattttgt atgttcacct ttcctatctc
     2641 aagaaaattt agggaggcat tgttttatta aagaagcttc tctgtatcaa taaagacaca
     2701 tttaaggaaa aacttttaaa aatctaattg ggaatttgcc cgcataaata agaggctgtg
     2761 acaaaagtct tgtcacagcc tcttattccg tataaacagt gttcaaaagc ttgcatcttc
     2821 gataatatgc ccaaccaaat gaaaaatatg agaagctatt tttgtttggc tgttcttatt
     2881 acttgatgaa gaacagcttt ttcacgacct ctttttaaaa gtctttttac agttttcgag
     2941 caaagaatga tacgattagt actaaaatga tggcaccaat caatgatggc acgattgcca
     3001 ttcctgcaat acttggtccc caagaaccta ataatgcttg accaagtgct gatccaacta
     3061 aacctgctac gacatttgca ataatcccca tcgaagaacc tttattcgtg attgctcctg
     3121 caattgctcc gataattcct ccgacgataa gtgaccaaat aaatcccatg tttcgttcct
     3181 cctaatgttt tacttcctta ttactgaacc cgtggttctg tattttcttt tactttttgt
     3241 gcgccgcttc cagctgctga ttttacttta tctgtttgat tcgaagcaaa ttcaccggtt
     3301 tttttcgctg cgtcagttac tttgtcttgc accgtttctt gatctttttc aaattcttct
     3361 tttgttttga tgtccactac gttagcgttg acttcgatca cttctaaatg tgtcatattt
     3421 tttacttctt tgccgattac ttcttgcatt tcttcaaaga ttttttcgat atctttgcca
     3481 tattccacaa tgacatccat atcaactgct acttgttttt taccgacttc tgtttcgatc
     3541 ccagccgtta cattatctgt attcacaagt tttcctgcca cattagagaa gaaaccaccg
     3601 tttgcagata ataaaccgtc tacttgttca attgcgattc cgatgatttt ttgcacaact
     3661 ttatcttcat aattcagctc accgcgcatt gaatcttcct gttcagaatc atgctttcct
     3721 tggttatttg tatgatcaga accaagtttt ccttgtccgt ttgtgtggtc agaactaagt
     3781 ttttcttgtg ctttcgcatg gtcagaagga tgtttttgct cttccacagg tttagaagga
     3841 tcgattgcat catgttgttc tggaacttta gattctggag ttcctggatg atgaggtgca
     3901 tgcttttctg catcatgttt cttctcttct actccttgtt gaacgttagg gactactgga
     3961 tctggatgtg cagatactgg tgctgttttg ttcttttcat cgttcacacc atttgattgg
     4021 tttggtgtcg ttgggtctgg atgtgcagac actggttttt ggttgttcat tttattgtta
     4081 ttatttttgt tattgttatt catggatgtt tcctcctaag atttaaaata gtgatctaag
     4141 attttcgttt gtttcagata aaaaccgata gcgacgccga tcgctgccat gatcaagatc
     4201 aataaggtct tgaaaaagcc cacggtgatc cataaaacgg ctaatatcaa tccaagtacc
     4261 ccaaagatga tagggagctt gtatgtcgaa aatagttctt tcatttttgg tcccccccta
     4321 ttctactcgt ttgcggtcat tgcttcgttt agctggttct tcaaagcctg tgtatttcac
     4381 cgatactttc actggctctt ttgttcccaa tatgtgttgt acttcatttt cgatttcttt
     4441 catcaatgtt cctgttttac caatgagtgc tgaagttcgt ttcaactccc cttttacatt
     4501 gacggcgatc ttgtttttcg ttgctttgac atttactttt ggtgaatgga tgaattcatc
     4561 ttcatgcaag tgagaacgaa ccattccttc aattgctttt ttatccaacg tcaactcccc
     4621 atcaccatgt ttcagtagaa agtttccgat tgtttttgga taaaacagga aaacaagtaa
     4681 cgtgataatt gtcaaaatag caaatgcaac ggcaagccaa aatacatatt gttgcacgta
     4741 tgaattagtg atggaaaaga atcgaaactt ctctagctga aaaggcaatg gataggttgc
     4801 acttaatagt atcgcaaaca aaagcacaga tagcaaaagt aatgttccga tcactccgat
     4861 cgtttttaca cctttattca ttttcgcacc tcctcatagg ggtgaaacga tcaacttctt
     4921 ataagttgat cgcttctcca ccagttactc cgtaaatttg ggctgtaaca taacttgctt
     4981 tattcgatgc taagaaaaca taaactggtg ctaattctcc tggctgacct gcacgttcta
     5041 gtaaactttg ctgaccaaat tcagggatcg aatcagaagg ttgtccttcg tctagttgta
     5101 atggtgtcca gattggacca ggtgctacac catttacacg gattccttta ggtgcaagct
     5161 gttttgctaa accgatcgta aagttagcga ttgaagcttt tgttgctgcg taatctaata
     5221 aatgttcgct tggattgaat gcttgaacag aagttgttgt tacgattgct cctcctgctg
     5281 ggatatgcgg aacagcttct ttcactaaag caaacatagc gatgatgttt atcataaatg
     5341 tgtctttgac ttgctcaagt ggtaactcag caatagactg acaagaaatt tgctgtcctg
     5401 cgtttagtac catcgtatct agcccgccaa gttcactcac ggctttatct atgatttctt
     5461 taggtgcttg ttcgtcacga agatcaaaag gtagtaagac aatcttacgt cctgcatctt
     5521 tcacgtattt tgctacttcc tctgcatctt tttcttctcc tgggaagaat tgaagagcga
     5581 tatctgcccc ttctctagca aatgcaatcg ctgctgcacg accgatccct gaatctccac
     5641 cagtgattag tacacggcgg ttttctagtt gattattgcc ttcataggat tcttctccac
     5701 aatcaggaac aggaatcata ttctgctgta aagctggtgt atcctgatct tgtttaggaa
     5761 atttctccgt atggtacaag tttcgcgggt cgattaattt tggttctgtc attgctgtat
     5821 tcctcctagt ttttaaattg tattgccaaa acaaatttct ttgtttgata cctgttttaa
     5881 atagattctt taatttgtat aatctttccg gtataaatgg taacctaaaa ctactgctgc
     5941 tatcaaagag ccaaagacta cgagtataaa gcctagagca gaacttttac ccggtaatcc
     6001 tcctgtattc attccccaaa tccctgcgaa caacgccgga acggaaatga ttaatgcagc
     6061 tgaatctaga tatttcatga tatgatttag attattgtcc atcatcccgt tgaatagatc
     6121 accaatactc tctaataaat ctcgatagat ctcgatcatt ttttctgttt gtcgctgtct
     6181 caaatggaca tcatagagta gctgttcatc gtctaaacgt ttgatgaaag tttcagaatt
     6241 ccataattga tccattgttt cccgttgatc tcttaaggta tgatcgatat atacgatgtc
     6301 tctttcaaga tctgcctgtt tatacagttc ttcattttcc gttgttttac gagctgcttt
     6361 atccaaagcg tcaattcgct ttttcatctc tcttagttct ttcacgtaat gggtgtaaat
     6421 atctaataat gtatacgtga tgatcttttc aaacgagttt aaatgatttc catgctttgt
     6481 cagcagccga tccacaaatt cggtttttct atcgatacaa gtaatcaaga gattatcgga
     6541 aagtacaaaa gatattggcg ttaatttttt ttcgatacga ttatctgact gatctagatt
     6601 aatcactacc aaagaaaatg gatgctctag tttcgtctca taaaggcgct ccatccgaga
     6661 aacttcttcc ggatattcgg ttcctacgaa aatatcatca ggaagctcga attcttcgat
     6721 tgcttgctca atctcttcgg gctttgcact atcaagtacg agccaattat attttttttc
     6781 atctgtttct attacattct tttcttcatc cacaaagtag tatctcaatg tatacctcct
     6841 ttttggactt tctcgaaact attagctgaa gaagcagttc tttaatttga tattataatc
     6901 gagtactgcg atttcatttt ctaatgaaaa gctgtcaagc tgtttgttga agtccatgtt
     6961 caatcgttta ccgaaataat tttgctctaa tttagatgta tagatgtcca ataatggatg
     7021 ctcgatttta gtcgcatatt ctccaaattc cgaaacaaaa ggaatgattt gaaaaaagct
     7081 gccttttgca aaagcacctt tgtcccttag aaatggatcg gaatcattga taagcgcgag
     7141 tgcgattttt gtattttccc caaccttttc aattaacgtc tctactgcat ttttagacat
     7201 tgtaatttcc attcctactc cccctcttca gttgtggtct tctattattt aacaactaca
     7261 tctgtttttg gttgaacttt tgcatatttt ggatatttag ggaatttagc tacacaacgg
     7321 aaagccgtca tccctgcata tttttcgtaa tatttcttcg ttggcccttc aaaatccttg
     7381 ctccacggtt tcacatgtcc tgaaaaatgg ataatgtaag gattgttgcg agtttcttcg
     7441 tattcacgtt cacctgctgc tgttggatgt ttttttgctt ttgccatgat atagccttgt
     7501 gcattccaac gcggatgaag tggtaaccaa cggtcatgta agatagcatt taatgcatct
     7561 tggtcatgga atcgtaattt ttctggatta tgttcgatga aatctaatac tttttgcgta
     7621 atattttcat ctaaccattt tttcacattg atcagcatca aacctgagtt gaaataacgc
     7681 attgatttag ctgaaatttt cattttttcc agtcgttgat ggaacccagc gtcttcaaca
     7741 gctgctacga ttgaatcacc gaagtcaaga cgccataatt tagagatatc ttgtaaagcg
     7801 atcatgtcgc aatccatata taagatacgt tcaacttctg tgccgcggaa caattccgga
     7861 atcgcaatac gataataagc tgtttctgga atacgatcgc tgattaccac atttgtaaag
     7921 aattctttat tgattttcaa gaattccaca tctgagttca ttcgtgcgtt tttcactgag
     7981 aagcgtaaaa gctgtttact ttcaaaatcg atatcatcgt caatcacata gaaccgaaca
     8041 aatttagacg ggttgcaatt atctaatact gagacaaata atgcagctaa atgtggtacg
     8101 aattttgtgt tacaacttgc gactactgct atttcttttt tattcattga tctcgcctac
     8161 tttcgttaga gtactgtgtg ctaatttttt taagtataaa ttggtatacg gatgatcttt
     8221 caacgtattc caaggtttat catgtcctgt aaagtgaacg atcgaaggtt tttcatttcc
     8281 ttctttatac tgacgttcat atttttcatt tggtgctgga tgacgttcaa aaatcagcga
     8341 agtctgcatg ttccattttg gatgaagttg ttcccaatct tcataaagta cagcattcaa
     8401 agcatcttgg tcgtgataga tgattcgatc gccattttca ctgaggtaat ggatagtttt
     8461 ttctgtaatt tcttttttat tccattgatc aatgtcaatc accatcactc ctgaattgaa
     8521 gtacaagtca tcggaatcga ttcctaatct ttccaaagct ttcacttggc ctggatcgat
     8581 aatagcgccg attgtttttc catttaacgg ctcatcatat agttttacga tatcgtctag
     8641 tactaacaca tctgaatcta agtaaaggac tttcttataa tcctctttag ctaataagtt
     8701 tggtaaagaa attcttaagt aagcggtagt tgtgatatgg tcactgacta ggaagtcttc
     8761 gtagatatct ttttccactg tcaggaattg gatacttgca ttacttgaat atttatttac
     8821 cgtttcttcc aatccttctt tactatattc tgatagacca tcgtcaatca cataaaattt
     8881 tatgcgtctt gttttattgc aattttctaa tgcagttgcg atcatcacgc tcaagtaagg
     8941 agcataattt tcgtctgaag cagttaccac aggaactacg ccgtatctct tctccatcaa
     9001 aatcactcct ttttctaatc tttttctaac ctgttctaca tctagaatat tcctcatttg
     9061 gttttaaagc aaaatatatg ctttgttttc attgaatatt ttaaaatttt tttatcacaa
     9121 aacgcatcgg taagcgctaa ctattcgttt ttcatggctt tttattcaaa ggtattgacc
     9181 aaaaactact atatatagcc acctttttct taagaatgtt ttttgaaaaa attcataatg
     9241 acaaagcaag ttagtttcat ttttttaaat tctcatttaa tcttaaggta acaggaaaat
     9301 agatatgtaa aaagaaagta ggaagataaa atgaaactag cagcgattga tttagacggt
     9361 accttgcttg atcgaaatgg aaagatacca gaagaaaata tccaagcact tcggaaattt
     9421 gatgaaaatg gcggaatcgt aaccgtcgct accggaagga acagcatttc tgctaaagat
     9481 attttttccg aattaggaat cggtggctat ttgatttctt ccaatggttc actgattgct
     9541 gaaatgaagg atggaaaaat ggcgaaagtt ctcagacgtt caaaaataga tagcgataca
     9601 ctgagaaaag ccttcttcta tgctcaagaa gcgaaaatat ccatcattgc tagccgagaa
     9661 acccaagatg atcagctgac attcgatgaa tcaaatctag tcaaagatga cccttattat
     9721 gaacatttca atttacagaa ccattctttt gatcaaattt taaatatgtt ggaagatcct
     9781 tctcttagct atcttaaatt agcattaaca gataagagtg aagaaaaact acagaagcta
     9841 caaagagatc tgcaagcaga tggtatccaa tcagtattct ctgatccgca tttcttagaa
     9901 atcacgccta caggtatcac taaagctcat tcacttgttt ttctggcgga ttaccttgga
     9961 atcgaatcga gtgacatcat ggcttttggc gatcaagaaa acgacattgc tatgttgaat
    10021 tacagcgggt tgagtattgc gatggggaat gcacagcgac aagtaaaaga aattgcaaac
    10081 caagtaacac tgacgaatga agaagctggt gttgcttata ttttaaaaaa cttctattaa
    10141 aaagaataaa atgacgatat gaatgtagga ggaaagaaaa tgttagttat tattgatatg
    10201 caaaatcata ttcttgatcc aaacagcgaa ttttatattc cagagtcaga tcaattggca
    10261 gaacgtattg ctcaaagatt agctaaagca cgccaaaata atgaatatat tctcttcaca
    10321 cgggatatac cagttgaact gaaggacaaa gaagaagaac gagaagattt gcaattgatc
    10381 cctcttcttt ctccacaaga aaatgaacga gtgattaaaa agtattactt tacgattcca
    10441 cctgaaactt tagctgaaat ccaggaaact ttatttgaaa gcaaagaaga acaaaaagag
    10501 atcgaagtag tcgggattga aacaaacttg tgtgtactct ctaatctgat aggcctgcag
    10561 agtgcttttc ctgaagcaga cttttttgtt gatccaatgt tagtctccag tagaaaacac
    10621 ggagaatccg ctttagagct gttgaaagat ttcaacgtct ctatcgtcga atcataaaaa
    10681 tatctatctt catttacgat atttaacaca caataaaaaa agcacgcaga actgttttag
    10741 tcctacgtgc tttttctatc aaagtagatt ggctttttta tgacacagag tgaaacggca
    10801 ggctcacgac ctctttctta aatggtgttc aatcagctga ccctcggtat tagcttaaca
    10861 aacggcaaaa tatgaggaac tatttttccg tttgttaagc taatactcag gtcaaaacgc
    10921 tgatttcaca acctcttcct taaacggtat tcaaaagctt gcatctgcag taataagaac
    10981 agccaagtaa aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg
    11041 cagctttttc acaacctctt atgtaccttg taggactcga acctacgacc ggacggttat
    11101 gagccgtctg ctctaaccaa ctgagctaaa ggtacatcat aaataaaatt tatttttgtt
    11161 acttttcaac tatagcacga atgacaaact ctcacaagtt tcaaaatata agtaaaataa
    11221 tattgttatt cttatttctt ttacttgtgt cataactatt ttgttacttt aatttttatt
    11281 tgcttatttt agaaaaaatg ttattctttt atttattctc aatgttagga gtgattattt
    11341 tgccatttat tgcgatttta ttcgatttag ttgcggcagc agcttatttt ctccaattaa
    11401 attatcaaac ctcaggaatt ctgtttattg gaatgatttt tcaaggtgtg attacccttt
    11461 tgcttcttgt catgatgctc agctataaag gaaaaagata tgcccgtatt caaacaaaag
    11521 tatttgttaa atatgtttcc attcgctatg ggatcatcat tctttccttt ttagtgaatg
    11581 cgatcgtcct ctttttatat gtattgaatt acttagatat caatccattg attttttctc
    11641 gatagataag acacattcag cggaacagtt cctgcttatc agcagaaaac tgttctgctt
    11701 tttcattttt cattgatcta ttcttctttt attttcctag catttcattt gtgatgtatg
    11761 caataacatg ttatctttta ttatatgaat cgttgaggag tccgttacat gattacgttg
    11821 gtgagagttt tgttttgggt gcctgcggtc gcactggtcg caagtattgt ttatcttatg
    11881 aattggaaca aagaacggtt ttatttagct attttgactc ttccggctat ctactttatg
    11941 tggaaagttt ttaattacaa ctactttgaa cccgatagtg tttttataga agaattatca
    12001 ggactggttt tgtctttatt gatcgtcatt ctttatttga tacgtttaaa taaaaaacat
    12061 tgagtactat tgattgcatg tgtagctttc tatagtcatt ataactattc tactataatg
    12121 aaaggtgatt aaatgagtat cgaagaagct attttactgg atactttacg ttgtcgtctg
    12181 aatttcttaa gaactggccg atctttatct caagtagctg tttctagcgc gttaagtatt
    12241 cctttattca aatatcgtaa gtacgaagaa ggagaagaaa ttccaagcat cattgaatta
    12301 atctatatcg ctgactttta cgatatttcc gttgattacc taattggtcg ctcagaaaca
    12361 gcaagcaaac tgaagtgatt ttttccagcc ttggtgctgg ttttttcatt taacgtctaa
    12421 ggttaacaat ctatatataa aggagacatt tctatgcgat tatggcatga aaaattgatt
    12481 tcaaagcttc ctcgtcaaca gcttttagga caacatcggg aatgctgtgc attaagagga
    12541 aatggctggg gaaagaaaca tgctactgtg gattatgttt ttcactattc tccttataga
    12601 ttattccaat atcatcagct tgtcatggat gaaatggaaa aacgtggtta cagcccagca
    12661 ccagagtgga aaaatcctat gtatcgtggc aaatcatgcg aagcattttt tgatttacct
    12721 actgtagagc gcaacgtgcc gatttatccg gaacacgatg aagtttatct agcagaatgt
    12781 ttagcaaatt tggagcaaaa aggaatttat cttacctaat ataactagaa aagccttcta
    12841 tctcctctta cttcattaaa aagctttaac catcttagat caagctaaaa tgtcgtaagt
    12901 tttagggtta tcttcatcct cacgtgcaaa actaaaagcg tactctagtt ttggttcatc
    12961 gccaacttcc ctagacataa atgaacatct cctgacgtgg tttaaaagta ccgcatcttc
    13021 gacgtctgta tacgatgcac agcacagatt gcttcttttg atctttcttt tgatttttct
    13081 gttgcttctt ctacaccttc ttcgattact acatcgatgc ttacaattgc ttctgcctaa
    13141 tattcccatg catcgtcgat ttaagcctct gtagtcactg tgatcgtttc cacattttaa
    13201 ctcctctgtc gtggcttcag ttgattttgt gatcagttca gttgctaagc cagagtcaga
    13261 acaacctgcc attgctagcg ttaatattga tagtaaaaat agttttttta tcataaggtt
    13321 ccccccctga aaatagacta aaataataac gctttctttt tttgttttca gacgaaaatt
    13381 aataatttct aaatattctt taaaaaagct tataactatt atgccttttt taatatataa
    13441 cacagttatt tttaaaaata agaatgctaa atcactctac aataacggcg aaagcacaaa
    13501 aaataaaaaa attattacgt tattaaaaaa acagatgaaa gatcaggttt tattagatct
    13561 cattctttca tcacttgaag ggatttaatg aactactttt tcctctttgt cgtttttata
    13621 tattcgtatt ccttcaaaca gtaggatagc ctctttcaat gcttgatatt tgaactgtcc
    13681 gttacttaaa aatttagatg tgccgccctc ttcctagaac atattctttt ttactttttc
    13741 attaattgac agaccaatcg aacgaaactg taaatttcga taggataatg attggtcaaa
    13801 ctcattcatt ttcaagacat ctcgattcat attcaagata ggaatctttt tatcaacatc
    13861 actttattcg tattttcctc taccataaaa gcttcacgct ccacaaaatt gttttataca
    13921 ctactattca gcccattcaa cgaccaatga attttttcgt acaaaaaaac cgtcttttct
    13981 ttcgaaaaga cggttaaaag tcaattttta ttaagcttta acaatgtttg ttgcttgagg
    14041 gccacgttga ccatcttcaa catcgtaaga cactgcttgg ccttcttcta aagttttgaa
    14101 gccgtcacct tggatagctg aaaaatgtgc aaatacatca tttccatctt caccagtgat
    14161 aaagccaaaa cctttgtctg cgttgaacca ttttactgta ccgttattca taaataaatt
    14221 cctcctgata cgtatcaatg tacgtttttt cgttgcaata aatatttgat aggcaaaagg
    14281 agttttatct atgtagatat atcgctcaaa ttacgtttca aaatagatta ctcatttaat
    14341 atatcatgct ttttctaata acacaaggat aatgcaatga aatataaaat tcattgtctt
    14401 ctattgtctt cgaatcgaag aaaaagttga ataaccgcga ttgattcact ttttgttttc
    14461 ttgtttattt gcttttatct gcaacttttg actttaaaat ttcttaacta tgaaaacctt
    14521 tcaattggtg gtatacttat aaatggaatt tcgttatcaa cgcgttcgtt gcctttcagc
    14581 aagtatttaa agaaaggaga atggctatga cattctataa caaagatata caatatcgag
    14641 taaccttgga tacagatttg aatctatttg tcgtttttga caaagaaaat accaatcatg
    14701 tagcgacagg tgttacgatc gaacaagcag tgcaagaatt aaaaaaatct gcataaggag
    14761 tggaaaccct catatggacg attctcgttc ttctctttaa acgcatctac tggtagataa
    14821 cagtagatgc gttttgtgtt tgttctaaat tgttcactac aaataaggta gatacttcaa
    14881 aataaaaaat tacttctttt tcatctgttc cctcagtcgt gcaggacaat aaataaagag
    14941 gactagaaaa tagtcctctt tattagagat acaacccttt actttctaaa aatcgttttg
    15001 cttgatgcag gtcataaaat tcatagtctg ctttattgga ttcatataaa cttacatcta
    15061 ctttcaactt atgcttccca ttatgatcgg gcaaccctat caaatgatct ttgatgagaa
    15121 aagacaaaat gcccgctcgc cttgctgctt cgatccctgc aatcgagtct tcaaatacaa
    15181 gtgcttcact tggaggaata ttcatttttg aacaagcttt caaaaagatc tctggttccg
    15241 gctttccttt ttttacatca tcccctgaaa tgatttgatc aaacaaggca tatagatttt
    15301 ccatttttaa atacatttct acttttaccc gtgtattaga cgaagccacg attgtatgga
    15361 ttcctctttc ttttgcaaaa gataaaattt cttctgctcc ttttttcgta tatactctct
    15421 tatcttcttt cacgattttt tctttatatt gattgattgc ttctcgccat ttgatatatg
    15481 gaacatcttc tccgtacaaa atgcccattt gttctcttat cgccatttct gtttggccag
    15541 ttagtaaata atagacttcc ttacgcattt cataatcata cttttgtgct gattttacat
    15601 aagcacgata agctaaacgt ccagtttcta ataaaaggcc atccatatca aagatgaaga
    15661 gcttgatttt attcataatg ctctcccagt ggtttttcct tacttatcgc ctcatataga
    15721 ccttgcatgt aagtgagtcc cattgcacga tcatacaggc catatccagg catagccact
    15781 tcaccccaca cggttctacc atgatcaggg cgtataggac cgtcgaaccc ttcttgcacc
    15841 aacgctttca taatatgata catatcaagg gagccttctg tagagggatg tgccgtttct
    15901 ttgaactttc tttctcctaa aaactccacg tttctgaagt ggacaaaatg gatgcgcgaa
    15961 cctacctcat ggatgatttc aactaaatcg ttttccggat ttgcccctaa agatcctgtg
    16021 cacagcgtta gtccattata tggagaatct actgcattta gaattttctt caagtcgcca
    16081 agatttttcg tcactctagg aaaaccaaaa agttcccatg gcggatcatc tggatggata
    16141 gccattttta catctgcttt ttcgcacact ggaatcactt tttctaaaaa ataagtcaga
    16201 ttttcaaata attcttcttc ggtcattcct tcatacagtt tttccagctg agcgaatttt
    16261 ttcaaccgtt cttcttccca acccggcaat ttgaatccct ttgactggct gtgtatcaat
    16321 tgatacattt ctcctggctc catctgatcg acgattttct gatcgtataa aagagaatag
    16381 cttccatcgt tgttttcatt aaacaaatca gttttcgccc atccaaaaat tggtttgaag
    16441 ctataacaaa tcaatcgaac cccacatcga gacaagtttt tgattgttcg tatatagtta
    16501 tcaatatagt aatctcgttc atctgtccct gccttgattg catcgtggat cgctacactt
    16561 tcaattccaa gcagttccag tccttggctc tcaacagatg ctttcaactc ctcaatttct
    16621 gaaatctccc aaacatctcc aggcatttta gtcagcaaag ttccaattat cccatttatt
    16681 cccggtattt ggcgaacata ttttaatggg atcgaatccc ctttttcacc ataccatcta
    16741 aatccccact tcatcttttc agccttcctt ctaaaaatcg tcctcatcta attttttttc
    16801 ttccgctgaa gcatgccaaa cagttaaatt atcttttcct tgtttgacaa tttgttccac
    16861 cgcttcttct atcggtgtgt gaagcatctg atgattgatt gcttctaaca acattggcag
    16921 attcacgccc cctatgacat aagtggctgc tttcttttct tcagtcagtt cattgactgc
    16981 caagagcgct tgattgtacg gacttgcgct aattagatca acaaatacta gcatgggttc
    17041 tgctgtgttg atcatagtta atttttcttt gaccttctct ccgaatgttt ggatgtcctc
    17101 cccagactct aagcacattg tttcaatatg gctcgatgtc ccgacaatta cttccgccgc
    17161 atctttcagt ccttcgctca acttaccatg tgtaacgatc atgatttcca acattagtct
    17221 tctcactcct tgttttgcac tttttactta tcgaaaaaga gcaataatct gattggcatt
    17281 ttgccaatta aatgttttcc ctcgtaaatc tgttttggtc aaatagaaga actgcgcttg
    17341 cttttttctt gcactaaaaa tataattgac gtttttttct ttcaacttat caacttgttt
    17401 cagccactgt tctttatttg aaaaataatt tcccgaaaac tgtagcggtg tatctgcccc
    17461 aacagagtct agataaaaaa gaatagcctc ttttttcata cccgatctca cgctgtctaa
    17521 gcccttttct ccataacagc cttcatcaac aaacgcatac gctacactcg gatcatcaat
    17581 cgtacggatc atttctagca tcgttaatat ggatgaagta tttcgaataa aagtttgtct
    17641 gctaggccat ccgcgagctg cttttcccaa taagaaaaag tagctcccaa atgctagtac
    17701 agtgatacct gtttatacgg atagcagatc gaatacgtga tgaggatttt tgttatatat
    17761 catcattccc caccaaccta aactgaacaa caaaatgaag gacaataata tagagtagat
    17821 tgtttttttc ctttggtctt tccaatcaaa catgaaataa cttcctaatg catgaacggg
    17881 tgtatcgtaa taagtacaga taactttctc tgctttttcg atatctccaa cataaatatt
    17941 ttttgatcgg taggctagcg tatcgtaagc aatgactgtc acatctgttc tcatactgta
    18001 aatatctgat actaatgctg aaagaaaacg ctgtttactc ttttttgtac ggcgcttacg
    18061 gtaaatatag cgataacgaa attcccaatc acgaaacagc tcgttcttct tcattttttc
    18121 gccctcttta tcttttttct aagcatttgt ttatgacaaa tcaaacttgt tcaatacatc
    18181 ttttaatcct gttttaggtg cagatggagt ggcttgaaaa taaatattct ttacgccata
    18241 ttgttcattc atttctttca atttttcagc atctgattta tttaaataaa cagatttcgt
    18301 cactaaaaga tctgtatcag gatttttttg agcaatccct ccaatattca attcttccat
    18361 ggggattccg tgttttacta agtcgtaaat cgtttggact gtttttgcga tcaaaataca
    18421 attgtattct ttaaattgat attcatccca atagaattta gctttctctg gtgtaatgac
    18481 aattgttttt tgtcctgtac ggcttcctgc atttttgtaa agatctctca taaatttatc
    18541 ttttgctaca acttcgtcta caacaaaaat ggaattcact ccactttttt gagataacgt
    18601 taccattact tgcccatgga tcaaacgctc atccacacgc gctaaattga ctacacccat
    18661 cttattcacg ttcccttctt tttataaaat accgattcct gctagcacaa ttaatattcc
    18721 agtcagccat aataatgctt gtgtgacttt caatcctttt ttcgtataga acaagtacac
    18781 acccattacg acagctaaag gtaatatccc tggcacgatt tgatcaagaa tttcctggat
    18841 aacaaactca cgtccggata tcgcaaattt caatgaagat tctactttta catagtttcc
    18901 cgcaagcacc cccatcataa acaaccctaa aactgatagc atttcaatcg ctgtctgaac
    18961 accagtgttt tgcattaggc ctgtagcatt acggcccata gtgaaagctt gtcttgcgaa
    19021 aaatacccct agtaaaacct gatacaaccc aaagcagata aaaggaaaaa gtgcgcctaa
    19081 aggacttccc tgttgtgccc aaggtaccgc gattgcgata aaaatatatt ggacggttcc
    19141 tgaatcgatt gcatctccga ttcctgctag tgcccccatc aatcccgctt tcgtgttata
    19201 catcaagtca tcctgcatca agatctcttt ttcttcatac tcttcttctg ctctttgctg
    19261 ttccatagaa gacattaggc cagtaatcac accgccaccc cagcttaatt gagtgttgaa
    19321 gaacagtaac tgccgtttat atgctgcttt taaagagtct tcatctttat aaagtttttt
    19381 caagattggc atcattccgt atagtagtga tggcgccaag taacgttcaa aagagtgagg
    19441 aatttcgttt gcaaaatgcc atctcagata ggcttttgtt acatcttttt tcgtgatttc
    19501 ttcaggagca cctgtttgat tatccattat ttcagccatt tcccattcct cctaaattaa
    19561 aatccgtcat catagtcatc ttcgtcatca aaagatgctg aattgttatt gcttgtggca
    19621 ggctgtactt gtttcccttt ttgcgactgg acaaagatca aagcgataat aatcccaaaa
    19681 atggcataag taaccatcgt aatatctaaa gatttcaaaa caacactcat aaaataagca
    19741 aggaagaaaa agaccagata atcttttcga ccgataacgt atacagttgt cgcgataccg
    19801 attgccgcta accctccacc tactacttca agaatgtgga taacgactgt tccctctaaa
    19861 gctttgatta catcagcgat caatggcgcc cctaaatata aagcaatgaa aacaagcgga
    19921 acgaataata aaaatgaggc aaccgttgga taaagaataa ttgatcgggc catagctttt
    19981 gtattcaaat cttctacagc tttgtctgta tatttaccta aaaaagtatt gatgaaaaaa
    20041 cgaaactgat acaagtaact tccgagcaat ccgactggca ccgcaacggc aatcgctgct
    20101 tctggcgaca gatcccctaa taatgcgact ggtactgcga tagctgctgc aatagatggt
    20161 tcagctggca ttgatcctcc tggagaaaaa acccccatat aaatcatttg caaagcggct
    20221 gtaacaatca tcgcttgtgc aacttccccc acgatcaacc cgacgactaa tccagtcatc
    20281 aaaggagaaa aacgtaaggt caacgtggct cctccaccta accagcggga cataaccgcg
    20341 gctacccata aagcaatcaa caaactttgt actacactta atgtttccat gtgtcctcct
    20401 ccttcagatc tttctgatat aaccttccag ttccatcatt gactgtatca gtgatttctg
    20461 tgttacctct ataggaatca aatgcacttt ctctttcgaa tcaataaatt cagccatttt
    20521 atctattatc tgtttatctt tcggaaataa tcccatttgt tctagagaaa ccggcagttc
    20581 taattcccga tacaaaggat ataactcgtc tattttttcc cagcgacctt ctagtgccag
    20641 ttgatagaaa atcccatacg ctactttttc tccatgtaga aaacgatggc tttccggaat
    20701 atatttactg atagcgtcat gcatagcatg tgctgccgca tttcttgcgt atttatctcc
    20761 taatcctcca actaaaccag caacagcaaa gataatctct gaaagctgtt tgaactccga
    20821 tgtcagtttt ctttgtgcca tatcttggat tgctttagca gaatgattca aaatctcttc
    20881 tttgcagatc ttcgcagtat cttgtgctag cttcaaaaat gattcatctt gcaaaaaatc
    20941 ttgttctaag atgagctcag attcatacca ttttgctagt gtatctgcta acccggcaat
    21001 gaaatatgga acaggggaat caatcactaa ttggggatct gttataaaaa aagcagcttg
    21061 tctgtaaaag tgttctgttt tcccttctgc ttgtccattc tccttataca tgacagcaag
    21121 aggtgtccat ggagcacaat tactagctaa agtagggatg actccgaaat cgattcttgc
    21181 aagattggca ctataaccca ctagatcggt cagttttcct ccgccaacgc caactacgaa
    21241 atcgatttca tttttttgat gattgtagct attcgtctgg cttcagcata actacattct
    21301 ccagaataac gatgataaaa aaattgaatc gtttcgtcat tcaaaaatac taatttaggt
    21361 agcgcctttt cccatgatac cgttccgtgg gtgaccagta ctcttttcgc gccgtatgat
    21421 ttcaagagat cagaaacttt tgaaatacca ccttcttcat ataaataaaa ttgagggcct
    21481 gttttcactt ttaaaccgtt caccattttc ttcatcatcc ttttatatag ttgctctttg
    21541 tctggacaga acgttcagga agaaggtgct gactgatctg ttcgacatct ctgacacatt
    21601 tttcacccat tgcttctaaa cattcccttg tgtaggccga tatatgtggc gtaatcacta
    21661 cttgttcaaa cgctaaatac ggatgatctt gacgtcccgg ttcttcttct aaaacatctg
    21721 tggcatatcc tgcgatcttt ccgcttttca gaccattaat catcgcttct tcatcaacta
    21781 gagcgcctct tgcactatta gatagataaa cattttcttt catctgatcg acttctttat
    21841 cagaaatcat gtgatagttc tcttctgtca aattcgcaca taaacaaatg atatctgatt
    21901 gttccagtaa ttcttgaagt tctacttttt ttgcaccaaa gctttcgatg tataaatctg
    21961 atttgtaagg atcataagct agaacatcac agcgaaaacc gttacgcaaa atttcgatta
    22021 ctccgcttcc ggtatttcct gcaccaatca caccaactgt tttgttaaac agcgtcctac
    22081 cgacgaactc cgcccgatct tcccatcgat cgtccttgac tcttgcttgt gcttgtgtcg
    22141 tgcgtcgaag catggacaac aaattagtta tattattttc tgctacagca tttcgttcaa
    22201 ttaaagcagg tatgatagat acgattgtct ggtgttcttt tgctgctttt aaatcaatat
    22261 tgttgtaacc aataccatgt cgggaaatca atagcagttc atctttgttt tcaaaaaaat
    22321 ctcgtcgaaa aaatggcgta acacttgcaa taatgatatt gaatccttta agtaactccg
    22381 ctagttcttt tcctggaatc tcttgatcaa cagtaaagaa atgaacgtct ccaactgact
    22441 ttaatctttc caagtggtgt ggaaagactc ttccgaaact gctggaatta acgattgcta
    22501 tatgataatc tggcatttta ctca
//
LOCUS       DAYLZO010000038        22389 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.039, whole genome shotgun sequence.
ACCESSION   DAYLZO010000038 DAYLZO010000000
VERSION     DAYLZO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.039"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            122..556
                     /locus_tag="ACGVJG_001318"
     CDS             122..556
                     /locus_tag="ACGVJG_001318"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFJ6557285.1"
                     /translation="MYQQFEHYLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFN
                     APMMTIVAETAKKERYSPDFMATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGD
                     TVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKLEMGKKIV"
     gene            complement(1010..2701)
                     /gene="argS"
                     /locus_tag="ACGVJG_001319"
     CDS             complement(1010..2701)
                     /gene="argS"
                     /locus_tag="ACGVJG_001319"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFJ6557286.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(3121..4068)
                     /gene="arcC"
                     /locus_tag="ACGVJG_001320"
     CDS             complement(3121..4068)
                     /gene="arcC"
                     /locus_tag="ACGVJG_001320"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFJ6557287.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(4183..5202)
                     /gene="argF"
                     /locus_tag="ACGVJG_001321"
     CDS             complement(4183..5202)
                     /gene="argF"
                     /locus_tag="ACGVJG_001321"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFJ6557288.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(5293..6522)
                     /gene="arcA"
                     /locus_tag="ACGVJG_001322"
     CDS             complement(5293..6522)
                     /gene="arcA"
                     /locus_tag="ACGVJG_001322"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFJ6557289.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            6983..7684
                     /locus_tag="ACGVJG_001323"
     CDS             6983..7684
                     /locus_tag="ACGVJG_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFJ6557290.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(8293..9309)
                     /gene="tsaD"
                     /locus_tag="ACGVJG_001324"
     CDS             complement(8293..9309)
                     /gene="tsaD"
                     /locus_tag="ACGVJG_001324"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFJ6557291.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(9306..9770)
                     /gene="rimI"
                     /locus_tag="ACGVJG_001325"
     CDS             complement(9306..9770)
                     /gene="rimI"
                     /locus_tag="ACGVJG_001325"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFJ6557292.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(9777..10319)
                     /gene="rimI"
                     /locus_tag="ACGVJG_001326"
     CDS             complement(9777..10319)
                     /gene="rimI"
                     /locus_tag="ACGVJG_001326"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFJ6557293.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(10303..11025)
                     /gene="tsaB"
                     /locus_tag="ACGVJG_001327"
     CDS             complement(10303..11025)
                     /gene="tsaB"
                     /locus_tag="ACGVJG_001327"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFJ6557294.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(11216..12196)
                     /locus_tag="ACGVJG_001328"
     CDS             complement(11216..12196)
                     /locus_tag="ACGVJG_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6557295.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(12220..13704)
                     /gene="galT"
                     /locus_tag="ACGVJG_001329"
     CDS             complement(12220..13704)
                     /gene="galT"
                     /locus_tag="ACGVJG_001329"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFJ6557296.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(13716..14705)
                     /gene="galE"
                     /locus_tag="ACGVJG_001330"
     CDS             complement(13716..14705)
                     /gene="galE"
                     /locus_tag="ACGVJG_001330"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFJ6557297.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            14953..15120
                     /locus_tag="ACGVJG_001331"
     CDS             14953..15120
                     /locus_tag="ACGVJG_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFJ6557298.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(15182..16993)
                     /locus_tag="ACGVJG_001332"
     CDS             complement(15182..16993)
                     /locus_tag="ACGVJG_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFJ6557299.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(16990..17355)
                     /locus_tag="ACGVJG_001333"
     CDS             complement(16990..17355)
                     /locus_tag="ACGVJG_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFJ6557300.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(17518..17913)
                     /locus_tag="ACGVJG_001334"
     CDS             complement(17518..17913)
                     /locus_tag="ACGVJG_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFJ6557301.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(17931..18893)
                     /locus_tag="ACGVJG_001335"
     CDS             complement(17931..18893)
                     /locus_tag="ACGVJG_001335"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFJ6557302.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(18893..19105)
                     /locus_tag="ACGVJG_001336"
     CDS             complement(18893..19105)
                     /locus_tag="ACGVJG_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFJ6557303.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(19126..19824)
                     /locus_tag="ACGVJG_001337"
     CDS             complement(19126..19824)
                     /locus_tag="ACGVJG_001337"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFJ6557304.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(19844..20386)
                     /locus_tag="ACGVJG_001338"
     CDS             complement(19844..20386)
                     /locus_tag="ACGVJG_001338"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFJ6557305.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(20518..21522)
                     /locus_tag="ACGVJG_001339"
     CDS             complement(20518..21522)
                     /locus_tag="ACGVJG_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFJ6557306.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(21519..22388)
                     /locus_tag="ACGVJG_001340"
     CDS             complement(21519..22388)
                     /locus_tag="ACGVJG_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFJ6557307.1"
                     /translation="MFDFDRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTR
                     NPLADSGLLGINAGASLAMALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKM
                     GMNPLQLILAGVAISSFFTAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLY
                     ILAVLVSFSLGKAISLIHLSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSF
                     IGLLVPPVVRGMVGTDYRYVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIG
                     VPFLLIQVRREQR"
BASE COUNT         6541 a         4805 c         3870 g         7173 t
ORIGIN      
        1 ccgctaaccg atgggaaaat taaaatggga tcatatccaa cagcttcttt ccctcccttc
       61 tgtcaaaaaa caatggatag ctatttatgg acaactacct caggaaaaag aggtggagga
      121 attgtatcaa caatttgaac attatctgtt taaagatttg gtcaagcatt ctaccttgaa
      181 acccgacaca ttggaaacga tcgaacggtt gagaaacgct gggatcaata ttggttccac
      241 gaatggattt aatgctccta tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta
      301 ttcaccagac ttcatggcaa cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta
      361 tatgatccat aaaaacatgc agtacttcaa aaacaagtcg gttgatgaag taatcaaagt
      421 cggagatacg gtctcagata tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt
      481 aattgaaggt agttcgctaa tggggatttc ttatgatgag taccaaaaat tagagatggg
      541 gaaaaagatc gtttaaaaca gtctgttgct cttagattcc aacaagccgg cgctgatatt
      601 tgtgtaaaca atttaaaaga gttaacagaa tacattttgt acgctgacat gaatccagaa
      661 tgacttttat acaaaataag aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca
      721 gccaagcaaa aatagctcct catatttttc gtttggttag gcttattacc gcagatgcaa
      781 gcttttgaac accgtttatt cgaaaaaaaa aagggagctg tgacacgact tttgtcacag
      841 tccccttatt tgcaaacaaa cagtgtttaa gagacaacct ctccactaaa cggtgttcat
      901 tcagctgact ctcggtattt gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt
      961 tgtttctcta atactcggat cagaacgctg atttcacatt ctctttttct tacatcttat
     1021 ctggtgcatg aagtcctagc aaacgaaggt cttctttcaa taatacagtc actgcataaa
     1081 ctagagctaa gcgtgcttct ttttgttcat catcagctaa aatcttcgta tgagcatagt
     1141 atttgttgaa tgcttgtgct aatttgattg catgtttcgc aatcactgac ggttcgtatt
     1201 tttccccagc agacaagacc gtttcagggt atttttgaac taatttaacc acttcccagc
     1261 tggtatcgtc attcaatgca tagtctgctg tttctgaagg gacaaatcct gctttttcca
     1321 acaggctgac tgcacgagca tgtgtatatt gcacataagg tccagtttct ccttcaaatc
     1381 gaacaacttc ttccaggtta aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga
     1441 aaatgactgc cccaacccca acttgtttag ctacagcatc tttgttttcc aaatcagggt
     1501 ttttctcgct gatttgttct ttagcagatt ggattgcttc atttaatact tcttctaata
     1561 agacgatctt tcctttacga gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg
     1621 gaatatggtg catgtcgtca gaccagtcaa agcccatttc cttcaagaca gctttcaact
     1681 gtttaaaatg atagctttgt tcattcccta ctacatatag agattgtttg aagtcgtaag
     1741 tacgtttacg gtacaatgca gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg
     1801 acttcttaat caacgctgga ttcaggtcat aagcagatag gtcaacgatc tctgcacctt
     1861 tatcctcgtt cagcaaatgt ttttcttcta aaagtttgac gatttcatcc atcttgtcat
     1921 tgtagaaagc ttcaccgttc aacgaatcga aacgtacttc cagcagatca tagattttat
     1981 tgaattcttt catcgattca tcacggaacc attgccataa ttgaatagcc tctttatcgc
     2041 cttcttccag acgtttgaac caagcacgag cttcttcatt taattctggt tctgtttcag
     2101 ctacttcatg gaattggacg tataaacgaa gcaattcatt gattggttct gctttaacag
     2161 cttcttctgt accccatttt ttgtaagcga cgatcagttt tccaaactgt gttccccagt
     2221 cacctaaatg gttgatgcgg attggctgat aaccgatttt ttccataatg aaaccgatcg
     2281 aattaccgat aactgtcgaa cgcaaatgtc ccattgagat tggtttggca atattaggag
     2341 aagacatgtc gattggtact gttccttgat tcccgatgtt gctatcccca taatgttctt
     2401 tttctttcac tactgtttgc aaaacttttt tgctgataag ttctttattc atgaaaaagt
     2461 tcaagtaagg tccgacaact tcgatttttt caaagttggc agaatcgatt ttttctgcta
     2521 aatctgcagc gatttgctgt ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa
     2581 atgcaacatc tccgtgttca gctgattttg gattttctag taattgctcg acttgttcca
     2641 gtgttaaatc atctttgaca gcgtcataga cagcttttgc gacaagatct ttattattca
     2701 tctttattcc tcctaaagtg ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc
     2761 atacttagta tgccatcact agagacgaga tttcccgcgg taccactcca attgtcctaa
     2821 aaaaggacca gctccatctg atatcggtag acgaccgtct gtttaaaaca gaattctcca
     2881 agtgcgcttc gttgtatctt cttcattcgg cttccaccac ttccgaattc gctagccaag
     2941 aatgatacaa gtactctctt gttcaacaaa tcttagtctt attaaacttt cttgaattat
     3001 atcagaaaaa aaaacgaaag gctagtcgtt tcataaaaaa acaatccaga tggcctaaag
     3061 gagaagaaat accacctgga tagctataaa tcatttcgta aatacgaaag acttaacaaa
     3121 ttaatcagct acgataatcg ttccttcttc agaagctaaa agattctcga tattttctaa
     3181 agaagtgatg attgccttag cgtttggttt tgcttcaaca aatgcaatag ctgcttctac
     3241 tttcggaagc atgcttcctg gagcgaattg cttttcatca atgtattgtt tcatttctga
     3301 aacagtaaca gtttctaatt tcttttgatc tggtttttgg taattgacat acacattgtc
     3361 tactcctgtc aatacgatca gcaaatccgc atcgatgatt tcggctaatt tttctgaagc
     3421 aaaatcttta tcgattacgg cttctcgtcc ttctaatcca gtagctgttt ctacaacagg
     3481 aatcccaccg ccaccaacag agactgtgat aactccttga tcgaccagcg tttcgatgac
     3541 acgtgcttct ttaatcgaga tgggtttagg ggaagcgact acttttctcc atccacgtcc
     3601 agcatcttct ttgaatgtca cagtggagtc agcattcatt tgttctttgg cttcttcttc
     3661 cgtgtagaat ggaccgactg gtttacttgg atttttgaat gaaggatcat tttcatccac
     3721 aataacttga gtgactaaag aaaccacatc tttatcgatc cctttttcct tcagtacttc
     3781 gcccatggcg ttttgtaacc agtaaccgat agatccttct gtcattgcaa cacatgtatc
     3841 taaaggcatt gccggtgttt tctcagaatc agcagcttgc tgctgaatca ataggttacc
     3901 gacttgcgga ccatttccgt gggagatgat caattcatct ccttgttcga taaatttcac
     3961 taaatacttt gctgtttcca ttaacgcttc ttgttgggct tttgcacttg catccgtaga
     4021 caagatggca ttgcccccta aagcgacaac tacttttcgt ttggccattt cattcgtcct
     4081 ttcttcagtt ctaccgaatt ttctagataa caaaacgagc cagcgttttg tgattttccc
     4141 tcaatgctga ctcgtttttt cgtgagatga atgtggttat gcttaaacgc gagggataaa
     4201 caagttacct aatgttgctg ccatgatcgc tttgattgag tgcatacgat tttctgcttg
     4261 gtcaaattga cgagcatatt tgctgcggaa gacttcatct gttacttcca tttcagtgat
     4321 gccaaagcgt tctttcattt gttcaccgta aacagtgttt gtatcatgga aagctggcaa
     4381 gcagtgcaag aagatcaaac gatctgtgtt gtgtgttttt tctaccattt ccatattgat
     4441 ttggtaaggt ttcaacaatt tgatacgttc ttcgaattta tcttcttcac ccattgatac
     4501 ccatacatca gaataaagga cgtctgctcc gtctacacct ttatcaacat cgtcggtaat
     4561 catcaattgt gcaccggatt tttcagcaaa gccttctgcc attttgacga tttcttcttc
     4621 aggttgtaat tcttttggtg caacgatacg catattcgct cctaaaattg cacctgtgac
     4681 taataaagag ttagccatat tgtttcgacc atctccgcag taagcaacag tgataccttc
     4741 tactgttcca aagttttctt ggatagttaa aaagtcagcg atcatttgtg ttggatgcca
     4801 ttcatcagtc aagccattcc aaactggaac accagagaat tctgctagtt cctctaccat
     4861 tttttgactg aatccacgga attcgattcc gtcaaacata cgtcctagta ctttagctgt
     4921 atcttctgtg gattcttttt tacctaattg gatatcattt gcacctagat attcaggatg
     4981 tgcacctaga tcgatcgctg cagtagtaaa tgcagaacga gtacgtgtag atgttttttc
     5041 aaataaaagc gcaatatttt ttccttctag atagtgatgt gggataccac gttttttcaa
     5101 gtctttcaag tgttctgaaa aatcgatcaa atattgtaat tcttcttttg taaagtcttt
     5161 ttctgctaat aagcttcttc cttggaatac tgattctttc atattaaaca ttctccttta
     5221 gattgttttt atttaagatt aaagaggaaa tcccgacggc agctgacagt aagactgccg
     5281 tcaggaaaca aattacttca agtcttcacg gacaagtggt tggctcatgc agcgtggacc
     5341 accacgacct cgtgacaatt cacttgaatt gatttctaac actttgatgc catagctacg
     5401 aagcaattcg ttcgatacat aatttcggtt ataagtgact acaacacctg gagcgattgc
     5461 caatgtgttt gatccatcat tccattgttc tcgaggtgca acgatctcat ctccattacc
     5521 agttgggatc agtacaagat catctaatcc aagagcagct ttcatcgttt cgtgtaagtt
     5581 atctgaatga gtaatcttga tatcatcacc atctggttcg attgtaaaga catccatctt
     5641 accgtcttta ctttgaattg ctggatggat agtgaattta tcgtgatcta ccattgtaaa
     5701 tacggtatcc aaatgcatca ttgcacggtt gttcggaatt ttgattgcca acactttttc
     5761 aaatcctgaa ttttttgcga ataggtggcg tgctaatttt tctaaagctt tcgcattagt
     5821 tcgttgagaa atcccaacag ctactacttt atcgcttaag atcaattcgt ctccgccttc
     5881 gatatgatct ggattttcac gatccaacca tacttcgatt cctttatttg caaatcttgg
     5941 atgatgtttc aaaatatatt ctgtaaacat cgattcacga cgacgtgctt caaaagtcat
     6001 cttattgact gtcatcccat ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt
     6061 tggcattggg tccatgtaaa atggatagtc atcgtctgct gacagatcat atagactgct
     6121 agaacgtact tcgatatctt ttgtacgaac cccagccatg attttatcta ccatttcttg
     6181 cgtttccatt gatagaagga actcatgaag accctctctt acagcattcg atgcaatatg
     6241 agattcatcc aacattttat tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc
     6301 tgccgctaat ttttccaagt aaagagtctc gacgccttca ttttgcaatg tcttcgcaaa
     6361 gttgtcatgt tcttcttgag caatcggaag atatggaata tcatcgaaca acagacgatc
     6421 cataatatct ggtgtcaaat tttccacttc ttgtccaggc cgtttcagta aaactgtctt
     6481 taatttccct atttcagaga aaacgtgaat aggtttatcc atgttgtgtt gcctccttct
     6541 ttttgattac atgtatagga tatcctcctt ttgtaatcac tttcataatt ttgtctcatt
     6601 caaaatgaga cattcctcac attaatattc gataattaaa taattaaacg tttttctatt
     6661 tttaagcgta atttattcat tttttattct gtatacactt tcaaaaaaat atttattcat
     6721 tgaaaaaatt tttttgaata aattaaaaaa ataaattata ttatcgttgt tagcgtttac
     6781 tatatgtatt attgttgttt tttatacaca aatcattcgc ttcaaaacaa attattcaat
     6841 accattgtat attttttaca taataacaaa ttatatttgt aaagtattaa aacttgtttc
     6901 taatgttggt atgttaaaac tttacaaatt ctttaaaaaa agtgacaata aatatagtga
     6961 acaataagga gaggactttt taatgcgtgt taataaaatc gattttcact tttcaaaact
     7021 cagatatcaa cctgattttg aacatttctc ggatgaagag ttccaacaga tcaaggaaca
     7081 tactgttctt cgttcttata aaaaaggaca gatccttttc gacgatggtg atcccagaca
     7141 acggttgtat atcttgacca aaggactcgt tcgtttagaa cgttatgatg aaagtgcaac
     7201 ttattactac tatgactatg tgaatacaaa taaccttttt cctatgtcag gaatgtttgg
     7261 agaagagacc tacacttata cagctcaagc gatgactgat attgaaacat tctaccttcc
     7321 tgtttcctac tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct atatgataca
     7381 aaaaatttct aaagtagttg aaatacatga attgcgtatc caaatcggat tgacctctag
     7441 cgcctttgac cgggtaaaac aaaatttatt cattttgaaa gaagaattgg gcgtaccaac
     7501 aaaagatggt catatcacca ttccttatcc gatcacctta aaagaattag cagtgaatag
     7561 cggcacgaca cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt ccgggctgtt
     7621 agattatgac aaaaagcgtt ttactttctg taagacagct actactccag ctatgagaga
     7681 ataacgcgaa caaaaataag cagtgaccct gataaatatc agggtcactg cttattttcc
     7741 tccctaaaac ttctgaaaga tccggcagtt ttagatcact ggacaagact caaagatttt
     7801 ctatgtgtgt ttcagttatg taatattcac tgtttgcaaa aaatactgtt agattaacta
     7861 gatgaagcgt cttgtcctca tccgatctag attattcgat attcctcgac aataaacgat
     7921 tactcagtga gggcaatcag aggaacggat aatgataaat aagctgtaaa aaatagcaaa
     7981 ataacaataa tgattaaaag tatgtaaaaa atagggcttt gacaaaaacg tgtcacagcc
     8041 ctatttcgat taaacggtgt gtacctgtca actcttcggt cacaagtcat aatgttcata
     8101 aacatgagga gctgtttttt gaaattgttc cttgtgaatc agagttaaac gacaggctta
     8161 cgaactcttg attaaacggc gttcaaaagc ttgcatacgc ggtaataaga acagccaggt
     8221 caaaaacatg agaagccgtt tttcctgggc tgttcttatt acttgatgca gggcagcttt
     8281 ttcacaacct ctttattcag ataaaaccaa gcttggatca gcattcaact gatagctagc
     8341 aaaatttctt ttttgcattt ccacatgtgc tgctgctcca atcattgcag cattatctcc
     8401 acaaagtcga agagggggaa tcactagttc tacctcaggg agtttagctg ccaaagcatc
     8461 ctgcaatcct tcacgcaacc cttgattggc cgctacacct ccagctataa tcagttgctt
     8521 gactggataa ttttgacaag ctcgaagtgt tttagtaatc aatacatcga tcacacttgc
     8581 ttgaaagcta gctgctaaat catttttgtc tagatcttct ccacgctgtt gtgcattatg
     8641 caccaaattg ataaatgcac tttttaatcc gctaaaactg aaatcataat tatcttcatg
     8701 gatcatcgct cgcggaaaat ggtaattatc ttttccctga tgagctagct gatcgatttc
     8761 ttttccgctt gggtaagaaa gacctaatac tcttccaact ttgtcataag cttctcctgc
     8821 tgcgtcatct cttgtctccc caatgatttc atatgatcca tcttcttgca tatagactaa
     8881 ttctgtatgt cctccgctaa caagaagtgc catcaatgga aactggaatg gtttaaccag
     8941 acgggctgca tagatatgcc ctgccatatg gtttacagga ataagcggca attggtgtgc
     9001 ccaagcaaaa gccttagctg cagaaatacc aatcagcaac gagccgacaa gtcctggtcc
     9061 ataagtgaca gcgactgccg ataaatcttc tgccgacaca ccagcttcta ccagtgcatc
     9121 ttccaagcat aaggtgattt gttctacgtg atggcggcta gctacttcag gtactacgcc
     9181 accaaaacgt ttatgactgt ttatttgaga agcaacgatg ttagataaga tctctgttcc
     9241 atttctaacg accgctacac tcgtttcatc acaacttgat tcaatcgcca gaatcaattc
     9301 ttcattcatt tcatcgtctc cgtttttaac tttctgctca taatgatcgc atcttccgtc
     9361 ggattgcgat aatatgattt ccgtttacct aatatacgaa acttttctga ttcataaagt
     9421 tttcttgctg cttcattaga tagacgtact tctaaaaaaa catgcagcga atcttttatt
     9481 tgttccgtaa tcagtaattg taacaaatgc cgtgcatgcc cttttccttg ttctgaaact
     9541 gcaacagcaa tatttgtgat ctcgacttca tctattactt tcgagtatcc aataaagcca
     9601 cagatctggt tatcttcagc taaaagcaga taatcagagt gaggctgttg gatgtctgcc
     9661 aaaaactgca ttttcgtcca aggtgaacca tgtggataag cattttcgct aatcatccat
     9721 acgcattctg ccaagtacgc ttcatcaaaa tctttttttc tgcaaatcaa ctaatctcac
     9781 tcattttcta aaagttttat catatccaac gcatcttctt ttgtttcagt gtaatatctt
     9841 tttttgatcg ttgttgcttg aaaccccaat ttccgataaa gtctttgtgc atcttgatta
     9901 ctcatacgca cttctaatga catcgtctca cagcgattca ttatagcaaa gttctcgatt
     9961 tcgtcaataa gcagcgaacc gattcgttta cgctgatatg ccggtcgaac ggcaatattc
    10021 gtgatatggc aatctaaacc gaacgctctt gaaccgataa aaccaatcat tcttccatta
    10081 tcaaagatac ctagatatag atgaggaagc ggtgacttca attctgatat aaaggcactt
    10141 tttgtccatg gcaattcgcc attatagact tctcgttcga catccagcag ctctttgata
    10201 tcttctgtag tcaattctct tagtacgtaa cgacgatcga tttgttcgac tgttttttct
    10261 gtataaatca ttttatcttg aaaaaagctt ctggcatacg ctctaaattt tttcaacata
    10321 actttcgtct ccaggtgtgt gcgtctccaa ccatttttct tctgcttcta ctcgttttaa
    10381 atagcgagga agaaaagctt gtttctctgc aactggttca actaaagcac ctaattgagc
    10441 cacagttaca ccacttggaa tatcccatgc tggaactgta ttaatcatcg cactaggcaa
    10501 tatttccttt atttcagaga agaatttgtg acaatcactc ccgacgaaat aaatagcttc
    10561 attcagcttt tgtagttgtg tgaacaaatc agaagcagaa atatgtacat cttcaagtac
    10621 agtttctaat ctattttctt tccagcggta tgccccagcg tatacatttt ttcttcgagc
    10681 atcaaacatg ggcacaataa tattatccac accgatgcaa ttagccgcga tggttttcag
    10741 actagatact cctactagct ctttatctaa tgtataagca atcgtttttg ctgtcgttac
    10801 acctatacga agccctgtat aagaaccggg accatcagaa accgcgattc ggtcaatgtc
    10861 ttttggactt atttgaagat ctgcaaacaa ttggtcgatt gcaggcatca gtgttacact
    10921 gtggttgcgt ttgatatttg tttgtatttg tccaagaatt tgttcgtctt ctaccacacc
    10981 gatcgccaaa gtttggtttg cagtatctat tcctaatgta atcatatatt tgatcctttc
    11041 tgctatgaca ttctcgcctt ccattgtagc atactttttt attctgagga tgaaaatacc
    11101 aaaggcaaaa ctagtacaat aatacatgat tatctgctgg cgggcaaatt ttgccccttc
    11161 ctgaaacaca aaaaagctgc ggtatcactc cattttccca cagcttttcc atcttttatt
    11221 tcgttgattc tcgaataatc aactctgttt ccacaataat ctttcttgaa accggtgctt
    11281 tggaattact taattgaaga atcgtctcca ctgctagctc tcccatccat tcggtataaa
    11341 cttttacagt agaaagcgag ggcgttacat atttcgctac actgacatca ttgaagccca
    11401 tcacagaaat atcttctggt accactaaac ctttttcttg aatcgctttc agtgcgccaa
    11461 tcgctaatgc atcattagct gcaaaaagcg catcaggaac atgcggattc gtttttaaat
    11521 attgcttcac tgcttgatat cctgactcga cagaaaaatc tgcttcgata attggacctt
    11581 cgtcgtagcc tttttctgcc aaaacttgtt gaaatgccat aattctggga tcttttagcc
    11641 gctgatggtt tttcttcgta tattctgttc cagcaagcat agcgatatgt tgcgctttat
    11701 gggagacaaa gtgttcaatg actttttgaa cacttgactt aaagtcgaca actagagagt
    11761 cgattccttg agaagtgccg tcagaatcta ccaaaagcaa ggcattttgg gatcctttca
    11821 tttctaataa ctgagcttcg tcgaattttc ctagtgcgat cgttccatct gccggaagat
    11881 cactaatgga atcccacgtt tctttcaata aagtaacgtt tgcttcttct gccttttttt
    11941 cgattcctag gcgaatagat aaataataaa gatcttcaag ctcttccgta tcgtcgtacc
    12001 actggatcag gcgaagcgtt tgcttagtat ttgctgcatt tttacgatgt ttcgtataat
    12061 ttaagcgttc tgctgcttca aaaatttttt gctttgtggc taagccaacg gaaagatcag
    12121 gatcatagtt gagaactctt gaaacagtag caggagaaac tcctgccaac tgcgcaatgt
    12181 ctttaattgt tgccatttgt ctctcctcct tttttacgtt tataaaatcg cagtaaatcg
    12241 ttcaaacgct gcctgtcctt tttcatctcg cttgaatact ccagcgtctt ccagtacacg
    12301 tgcaaaaacc tgacctacag atttttgtac gacatcggtg accgtatctt tcgtcaaatc
    12361 aggatgttct ttttttaatt gttctgccca cggacgatga tattcagcca tctggttttc
    12421 ttctcctaat aagaaatgct ctacttctac tagttctggt ttcaaacgag gtggtaaaac
    12481 agcaagcccc attacttcga tcaacccaat gttctccttt ttgatatgtt ggacatcttt
    12541 atgtggatga aaaatcccat caggatgttc ttctgaaaca ttgttgtctc gaaggacaag
    12601 gtccaactca aagtaattgt cccttcttcg tgcaattggc gtaatcgtat ggtgtggtgt
    12661 accatctgct gaaaaagctc tgatagatac tgtttcatcc gaatatgctt tccatttttc
    12721 caatactgag ttagctgctt caataagcaa gtttttctct gttgattgca aacggatcac
    12781 agacatcggc catttcacga ctcctgcgat gacttgcgga taatcttcta gtttgataag
    12841 tttttctatc ggtgcttttg ccataggaaa ttcatgacgt cctgcctgat aatgatcatg
    12901 agaaagaatc gatcctccaa caattggtaa gtcagcattc gaaccaacaa agtaatgagg
    12961 caagacttca gtaatccgaa gcagtctagc aaatgtttcc tttgtgattt tcatcgggcg
    13021 gtgttgttct gataaaatga tcgaatgttc attgtaataa gcataagggg aatattggaa
    13081 tccccagctt tcaccgtcta gattcattcg aataatccgg tgattggtgc gggctggata
    13141 gttcaagcga cctctatatc cttcgttttc catgcaaagc atgcaggtag gatagtctac
    13201 ttttaccgcc tcgcgttctg ctgcgatttg ttttggatct ttttctggct tagataaatt
    13261 gattgtgatt tccagttcac catactccga ctctgccgga aatttgatgt tttttgcgat
    13321 ggctcttgtt ttgatgtagt catttttctt acttaaatta aagaaatact cagttgcttc
    13381 ctctgggctt ttcgaatagt gctgtgcaaa gaatgcattg acgactgaag gtggcggtgt
    13441 gagaaaatcc attaattgcg cttcaaaaat ctcttgttct gctggcaatt ctgaaagaac
    13501 accattttct cttgccttag cgacaagctg atccaataag tctacagatt cttccttaac
    13561 tggtcttact tcaatgcttt ctagagcatc ttctcctacc atccctaaaa gacgattctg
    13621 taaatataag cggtccattt ccatccatcc gccagcttcg atcgcaagtg tggcaaaatc
    13681 acaaatcgtt tgactgattg acatatctca ttcccctatt cttcataacc atttggatgg
    13741 ctgacatgcc aatcccatgc tgttttgata atcgctttga tatctgtgta ttcaggtttc
    13801 cagcccagaa tctctctcgc tttttcactt gaagcgacta atcggctagg atctccagct
    13861 cttcttggtg ctatttctgc tggaatttct tttcctgtca cttctctagc tgcttctagc
    13921 atttccttga ctgagtatcc tttgctgctg cctaaattga agaagttgct ttcatttcct
    13981 tctttcaagt attccaaagc taaaatatgt gcagcaatca aatcttctac ttgaacataa
    14041 tctctgatac atgttccatc tggtgtatca taatcgtctc cataaacagc caaggctttt
    14101 ctttgaccaa gagctacttg taaaatgatt gggacaagat gcgtttctgg tgtatgatct
    14161 tctccgatag atgcatctgc ttttgctccg gctacattga agtagcgcaa agcgacatag
    14221 cgcatgccat atgcttggtc acaccacttc atcatttttt ccatcatcaa tttactttca
    14281 ccatacggat ttttaggatt agctggtgta tcttctgtga ttggagactc tttcggctct
    14341 ccataagttg ctgcagtaga agagaatacg attttattca cgttgaactc gtgcatgact
    14401 tcaagtaaga tttgcatacc gtatacgttg ttgttgaaat acatcaatgg tttttcgaca
    14461 gattctccta ctaaagagct tgcagcaaaa tggattactc cttcaatcgg ttccttttca
    14521 aatacggaac gtagaaattc tttatcccgg atgtctcctt cataaaaatg agcatctgga
    14581 tgtactgctt gtttgtgacc tgtgagcaaa ttatctacca cagctacttg ataccccttt
    14641 tgaactagct ggtctacagc gtgcgaaccg atataaccgg ctccgcctag tactaaaatt
    14701 gtcataaaaa acactccttt gattagtaaa attgtgtttg ttgattctat ataatttaaa
    14761 aaacgaagtt tacttttatg gtgaaaacaa acaaagtaaa atttcgtaaa ttcatatggt
    14821 aaatttataa taaaacattt agtaaacatt ttcaagggtt ttttgcaaag gtttacaaac
    14881 aaacgtaaaa ctagcctttt tttctctttt gttccataat agatataaca aataataaat
    14941 gaggtggatt caatgaaaaa atttgtatct ggaatattag taggaagtct ggcaacagcc
    15001 gctgccgttg ctggtttggt tgcttctgtg aaaaaaacag tgatcgatcc aattgacgaa
    15061 aaagaagcaa tgatcgaaga aaaccgtaaa aaagcaatgc gtaaacgcgt ttctcgttaa
    15121 taattattta aaaatacgca tgaagacttt ctaaaaaaca gataagtcat catgcgtatt
    15181 tttatttgtc agtcaatata agttgtgcta ttttatctgc ctgtctcaga ttccatggat
    15241 ctgtataagg tctagctact gctgcagtta gccaatccac accttcaaca ggtattccat
    15301 agcaataaaa gtcagactgg atttcccctg aagcatcaat cacgcgattc gtacctcgct
    15361 caacatctat agcttctgaa tgataacttc ctgtattatc tgaaaatgaa aaagggcgaa
    15421 caagtttgtt ttccttcaaa ttttttatga caggattact tgaacgagaa aagcttgttt
    15481 gtggcaatcg agcttcaatc aaatagcgac tgctgatttc tttatcttgt agagaaataa
    15541 catagcggtt gtgcttttgc tgaatatcaa atggcggttc gatcaaatga aaaatacccg
    15601 catctatcag tgctgctaat tctttcgtcc gaatgattgg cggaccgatc gttaaaaagc
    15661 tatttagatg agtaaaccat ccccatagta tttctttgta ttccttagca gaaaaaacgt
    15721 gccattcaat agcctgccgg attggatcac gccagtcttt taatgcatca aatgttgaag
    15781 caaatgctcc tgtgcaattg cctttttccg cttcttttat ctgcccttca ataaatattc
    15841 ggctagcttc tgaaaagggc tcattctgat aggcaagagg agtttctaaa tacgaccaat
    15901 cccatctata agggattagt gctggatgct ttccgagaca ctcctctgaa gagagggaga
    15961 gaaaatcatg ctcaaaattt ttctttggaa tcggcaagtg atattcttcg atgattttct
    16021 tgtaataaaa caactcaacg tcttttttca gatagtcaaa aaatgtttcg ccagtcaaaa
    16081 atcccgactg atgccattgc tccaggtttt cttttgttag taaccgtggc catgccattg
    16141 ctccgccttg cttttgattt cttcctcgag ggtaataagg gagccccctt cttgatccac
    16201 tgtatacaat cggttccttg cctgatggat gatagaccag ttttcccttt tcgttctcga
    16261 agcgccctcc tcttgatact gtaaacaatc cgacataatc aaaaaaactt aagcctagac
    16321 ctcttaaaaa gacattttct ttaggcggta tcttcgaaag atctacatct gcaggattag
    16381 aaggaagctg atacagtaaa acatttttct ttgcgtagtc tacccacact ctttcttctt
    16441 ctgtctcatc attttcccag tgaccgcttg ctaaaacaag ctgatcgata gaatagcttt
    16501 gctgatctgt ctgtaaaaga taaccatctt ttcttttgct tacttttttt actaaagaat
    16561 aaacaaactg aatagaagct ggaaattctt cttgcaactt tgagaaaaac cagcgttgat
    16621 agagtccgta taaacatcga gtagattgct gatctttttc cagacgattg atttcttcta
    16681 taattagttg cttatttttg gttaccgtct gttcgatata atctgtagca ggtcctttac
    16741 tccattgaaa caagttgggt ccctgtgtta ccaatcctcc actggacaat gtttcatctg
    16801 taaaaagcgt cacttgttga gaaacagaat tcattaataa ctccggtggc tgattcaacc
    16861 gccaaactcg acctccagga ccattgggat cgaaaagaaa aagttcaaga gaatgtctgt
    16921 cctgattatt atggagaatc ctttctgcta cacttaatcc tctaggtccg gcaccaatga
    16981 tccctatttt catttgcatg cctccatttc gataaatttt ttcaataaaa cagcatggtt
    17041 ctgatgttca ttttttgcac cataaacaaa agtaatgact ggatgttctt tcataagttg
    17101 actgagttta aaaatagctt gcttcttttc ttcttcgaaa atcaattctt gctggtattt
    17161 ctttgcaaat gaagcaaatc gttctggtac atggtcaaaa gttttgcgtg cttcattact
    17221 aggtgcaatt tctttcaacc acagatctaa ctgcgctttt tcttttgaaa ttcctcgtgg
    17281 ccagaatcta tccacgagta ttctatagcc atcctctttt tcagcatcaa ggtaaacacg
    17341 ttttaatttg atcatagttg tttccttctt tctacctttt cgcttagctt atatatgtag
    17401 ataatagcac aaaataaaaa aaatgaacgg cacaactatc cattgcgccg ttcatgtgat
    17461 cttggtcaaa atctaatgat tgattcggtt tttaaagctg tcatacaatc atctgtttta
    17521 tactttttta cttttgattt ttccagacca tccgtcatat ccacccttaa gtaaataaat
    17581 ctcttgataa cctgctttac gtaatttatt tgctgtacgg atgctcaaac ttttcttttg
    17641 atcgtaaata tagacaggct ggtcttttcg aatagatcct aatgaatctt tcaaaacagt
    17701 gtaagggatg tttcttgcac ctaaaatatg cccagcatca aacacatctt tttcgcgaac
    17761 atcgattact tgtgcttttc tcattccttc tcgaaattct tcttctgtca aagtcgttgc
    17821 tgaacgtttg gccattactc gtagatagac ttcccaaagg atgattgcaa gcacaatcaa
    17881 tagcaaaatg atattaatta cccataaaac catgtaatgc ctccgttttt ttaagcaaat
    17941 ttcaaggcta atgaggcagc accaatgaca ccagcttcat taccaagctc tgctaatttg
    18001 atttttgttg attctcttac ttgcgggaaa gtaaattctt taaaatattt ctctacacgg
    18061 ctacgtaaaa attctcctgc tgccgatact cctccaccta acacgatact tgacggattt
    18121 aatgtattcc ccaagtttcc gcaagctaaa cctaagtaga agcatacgcg atcaacaacc
    18181 atcaatgcaa acggatcgtc ttcttgagcc atttcaaaga tatctttact tgttacttct
    18241 tctccgttgt ctagcattgc tttcagtttt gaatcccctg cgtattcttc tgccaagaaa
    18301 cgacctaggc gtaccacacc tgtagcgcta gatacagttt ctaaacatcc gcgtttaccg
    18361 caagtacatt cgaacccttc aggatccaca gtgatatgtc cgatttctcc tccagctccg
    18421 ctcacgccgt gaatcaaatt tccgccagca acgatacctc cgccaacacc tgttcctaga
    18481 gtaacgaata caacgtctgg atcgttttct ccagcacctt tccatcgttc acctaaagca
    18541 gcaacattcg cgtcattatc gatagcgaat ttgattcctg ttcctgcctc gatcagtttt
    18601 ttcacaaatt gaacttctgt ccagtttaag ttatacgcac caataaccgt tccagcttcg
    18661 ctgtccacgc ttcctggggt acccatgccg attccgataa agtcttcggc tttcatatta
    18721 tataaattca aacgatgatt gattgattca atgatttcag gaacgatatg cttaccatca
    18781 tccaaaatat tggtatcgat gctccatttt tgctgaatct ctccttctgg tgttaaaatt
    18841 gcaaattttg ctgttgttcc acctaaatca atcccaataa tcttcttgtc cattacaaat
    18901 ttcctttctt tcggctttct tcaattcggt gctctctttt taatatgcgc cacgctgtca
    18961 tgtatgtatc atgatcaatc aaacggctat catacagatt acgcaattcg atagtcatta
    19021 attcaatgtc atagatgcgt gcacccacat agataaagat gccaaatttt ttgaacagct
    19081 gctgtacatc atataagctc tccatcttta agcacttcct tcatattata caagtaaacc
    19141 atattttttt aaacccagta aaatacatat tacaaaaaga aatacaaaaa ctagtgtaga
    19201 aagaatcctt tggtgtatcg aatagcttgt ctgttgtttc ggtagaccca aaacattccc
    19261 taacactaat ccgccaatta gcccaccgac atgtccccaa acatctacag aggtatccag
    19321 aagaccaaaa acaaatgtca acacaataaa taaaagaaaa tgtctgacta gctgctggat
    19381 cgctgggtta tgtttaaaat gaaaccctaa gataaacaac gcaccgaaca aaccgaatat
    19441 cgaagtactt gcaccaccag acagaacatt cgcttcgtta aaagcaaagc ttgctgtatt
    19501 tcccattatc ccgctgaaca tataaatcaa gaaaaaacgc caatggccat agattgcctc
    19561 gatttgctgc cccataaaat acagtatcag tgagttcatt acgaaatgca tcagtccaaa
    19621 atggatgaag attggtgtca ctaaacgcca ccattcattg aagtgaacaa gataaggacc
    19681 atacattccg gcataataag ggatctcaag tcgcggaaat aattccatca tgaggaagac
    19741 cgctgtctgt atccctaaaa aagcataggt ccataacggt cgtcttaacc atgcacgcaa
    19801 ttttatttgc tgttgataat tcatgaaaac ctcccatttc attttagtca ataaacaatt
    19861 gttggatctt ctgatcaaag ctctccggtt gccattgttc attcaattgt tcacgaaaaa
    19921 caaggctaca tgttttttta tgaaaattat ttaaataccg gtcgtaataa ccaccgccaa
    19981 aaccgattcg ataaccggct ctattgtaga taatacctgg tacaatgagg agatcgatca
    20041 tctccggcaa atagagcttc tctgattgtg gctcaagaac accaaaactc gaacgtacaa
    20101 gaacggactg ttcatccatt tcataaaacg ccatctgacg tttcggcaaa gttttaggta
    20161 caactatttt cttttgttct ttttctgctc gttggataac cggctgtgta tccagttcaa
    20221 attcattcga taaagtcgtc cctatcactt ttgctgactt ccagtactcg ctggcgaata
    20281 gctgtttata gattcgctgt tctttttcct ttttcttgcc acttttggcc agatttttca
    20341 gcttttcgat tcctttccgg cgtaatctta ctttcaattg gtccaaaaac tcacccttcc
    20401 ttctctcata aaatggtata ggaaaaaaag aaaaatagca atcgaacttt caaataaatg
    20461 cataaataga ataaaaagaa gacctagaaa tgattggcct tcctttttta atgaatttca
    20521 actgttcagc gcgtgttttt gtctgataaa aagtgcatac agaaaatatg gcgcaccaaa
    20581 aagggcaatc aaaattcctg taggtacttc cccacttggt agaatcactc tagcaaccat
    20641 atctgcacta agcagaaaaa tggaaccgaa caaaccgctc agaacaaagg tccagttatt
    20701 ttctttacgt acgactagct tcgcagcatg aggagctagt agtcctacaa aagataaact
    20761 acctgtcacc gctacactac cgcttgccaa agcaacagct aacaacaaaa ggattttttg
    20821 acttttagcc acgttaaggc ctaatccttg cgcgcgttct tgaccaaatg atagcaatac
    20881 aatcctctgt gcataaagtc ctcctagcgg aattaagata accgcccatg gaagcaatgc
    20941 ccaaacataa ggccagctcg caccccagat tgatcccgct aaccatgcat tcacaaatcc
    21001 ataattttcc ttagataatt ggatcgtgca tagcaatgtt aatgcagaaa ggccggcatt
    21061 aacggcaata ccggacaata agattcgatt catacccaaa aagccatttc gatcataggc
    21121 cgcaagatag acacagcaag cagctaaaac accacctaga aaggctacaa ctagcaatag
    21181 aaacggcagc gaatagtgcg aaaagaaacc taaataaagc atcactaaaa atcctgctcc
    21241 ggcattaatg cccaatatac tagaatctgc caattcattt cttgttactc cttgcaagat
    21301 ataaccagaa acgccaagac aagctcctgc taataaactg atcagtaatc gaggaactcg
    21361 aaattctcta acaatcagcg ccaaagaaga tgattctttc cctatgaacg tggcaaaaag
    21421 ctgatccagg taaataaaag gcgtaccaat cattaaacta cctatagaca ggatgacaag
    21481 tacaaaaagc aagaaataga acgtccaata tatttttttc accgttgttc cctccttact
    21541 tgtatcagta aaaacggaac gccaatcaat gcaatgacta atccaaaagg tgtttcaaaa
    21601 gggggattga tcatcctcgc tgtaaaatcc gccaacataa ccaatccacc gccaactaaa
    21661 aaagagacag gtagaacata acgatagtcc gttccaacca ttccgcgaac aactggcgga
    21721 accaacagcc caataaatga aacaggtccg actaacgaaa cggcaatggc tgctaacact
    21781 gcaactagac agaaaacaat caaccgcgtt cgtttaggat gacttccaag agctaatgca
    21841 tgtgtttcac tcaaatgaat caaagatatt gcttttccta atgaaaaaga aaccaaaaca
    21901 gcaagtatat ataaaggaaa caatactgct aattgtttcc atgtgatatt ggcagctccg
    21961 cctacaaacc aaaaagcaag atcctgctgc agattaaaga attgggtaag tgcttggctg
    22021 atagcagtaa aaaaagaact aatagctacg ccagctaaaa tcagctgtaa aggattcatg
    22081 cccattttcg aaaactgcaa aaaaccaaat acgagcaaag ttacagcaaa agaacctgcc
    22141 aatgacatta gaaacacaga aaaatcagaa gctttaggcc aaaaagcaaa actaagggcc
    22201 attgccaacg aagcacccgc gttgattcct agtaagcctg aatctgctaa aggatttctt
    22261 gtcactcctt gcatcaatgc accgctcaat gcaaaacaac tccctacaag aaatgctccc
    22321 gccacacgag gcaaacgaat atttcgtatg atttggtgac tttgttttga tctatcaaaa
    22381 tcaaacaac
//
LOCUS       DAYLZO010000039        21500 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.040, whole genome shotgun sequence.
ACCESSION   DAYLZO010000039 DAYLZO010000000
VERSION     DAYLZO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.040"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(29..1261)
                     /locus_tag="ACGVJG_001341"
     CDS             complement(29..1261)
                     /locus_tag="ACGVJG_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFJ6557308.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACGVJG_001342"
     CDS             1516..2085
                     /locus_tag="ACGVJG_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6557309.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACGVJG_001343"
     CDS             complement(2263..2703)
                     /locus_tag="ACGVJG_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFJ6557310.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACGVJG_001344"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACGVJG_001344"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFJ6557311.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="ACGVJG_001345"
     CDS             3657..4580
                     /locus_tag="ACGVJG_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFJ6557312.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="ACGVJG_001346"
     CDS             complement(4805..5044)
                     /locus_tag="ACGVJG_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557313.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="ACGVJG_001347"
     CDS             complement(5049..5468)
                     /locus_tag="ACGVJG_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFJ6557314.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACGVJG_001348"
                     /gene_synonym="mefH"
     CDS             complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACGVJG_001348"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFJ6557315.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="ACGVJG_001349"
     CDS             complement(7040..7675)
                     /locus_tag="ACGVJG_001349"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6557316.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7789..7881)
                     /locus_tag="ACGVJG_001350"
                     /pseudo
     CDS             complement(7789..7881)
                     /locus_tag="ACGVJG_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8017..8652)
                     /locus_tag="ACGVJG_001351"
     CDS             complement(8017..8652)
                     /locus_tag="ACGVJG_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6557317.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="ACGVJG_001352"
     CDS             9164..10306
                     /locus_tag="ACGVJG_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFJ6557318.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="ACGVJG_001353"
     CDS             10299..10505
                     /locus_tag="ACGVJG_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFJ6557319.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="ACGVJG_001354"
     CDS             10584..11777
                     /locus_tag="ACGVJG_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6557320.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="ACGVJG_001355"
     CDS             11982..13121
                     /locus_tag="ACGVJG_001355"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFJ6557321.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="ACGVJG_001356"
     CDS             13114..13914
                     /locus_tag="ACGVJG_001356"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFJ6557322.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="ACGVJG_001357"
     CDS             13914..14741
                     /locus_tag="ACGVJG_001357"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFJ6557323.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14746..15453
                     /locus_tag="ACGVJG_001358"
     CDS             14746..15453
                     /locus_tag="ACGVJG_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFJ6557324.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="ACGVJG_001359"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="ACGVJG_001359"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFJ6557325.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="ACGVJG_001360"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="ACGVJG_001360"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFJ6557326.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="ACGVJG_001361"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="ACGVJG_001361"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFJ6557327.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="ACGVJG_001362"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="ACGVJG_001362"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFJ6557328.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="ACGVJG_001363"
     CDS             19038..21071
                     /locus_tag="ACGVJG_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557329.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..>21500
                     /locus_tag="ACGVJG_001364"
     CDS             21115..>21500
                     /locus_tag="ACGVJG_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFJ6557330.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFS"
BASE COUNT         6824 a         3838 c         4237 g         6601 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc
//
LOCUS       DAYLZO010000040        21370 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.041, whole genome shotgun sequence.
ACCESSION   DAYLZO010000040 DAYLZO010000000
VERSION     DAYLZO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.041"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(36..437)
                     /gene="ftsL"
                     /locus_tag="ACGVJG_001365"
     CDS             complement(36..437)
                     /gene="ftsL"
                     /locus_tag="ACGVJG_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFJ6557331.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(442..1401)
                     /gene="rsmH"
                     /locus_tag="ACGVJG_001366"
     CDS             complement(442..1401)
                     /gene="rsmH"
                     /locus_tag="ACGVJG_001366"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFJ6557332.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(1422..1853)
                     /gene="mraZ"
                     /locus_tag="ACGVJG_001367"
     CDS             complement(1422..1853)
                     /gene="mraZ"
                     /locus_tag="ACGVJG_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFJ6557333.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(2023..2391)
                     /locus_tag="ACGVJG_001368"
     CDS             complement(2023..2391)
                     /locus_tag="ACGVJG_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFJ6557334.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            2562..3518
                     /locus_tag="ACGVJG_001369"
     CDS             2562..3518
                     /locus_tag="ACGVJG_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFJ6557335.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            3591..3707
                     /locus_tag="ACGVJG_001370"
     CDS             3591..3707
                     /locus_tag="ACGVJG_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFJ6557336.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(3762..5444)
                     /gene="recN"
                     /locus_tag="ACGVJG_001371"
     CDS             complement(3762..5444)
                     /gene="recN"
                     /locus_tag="ACGVJG_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFJ6557337.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(5462..5911)
                     /locus_tag="ACGVJG_001372"
     CDS             complement(5462..5911)
                     /locus_tag="ACGVJG_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFJ6557338.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(6018..6836)
                     /locus_tag="ACGVJG_001373"
     CDS             complement(6018..6836)
                     /locus_tag="ACGVJG_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFJ6557339.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(6845..7735)
                     /locus_tag="ACGVJG_001374"
     CDS             complement(6845..7735)
                     /locus_tag="ACGVJG_001374"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFJ6557340.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(7737..7961)
                     /locus_tag="ACGVJG_001375"
     CDS             complement(7737..7961)
                     /locus_tag="ACGVJG_001375"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFJ6557341.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(7966..9303)
                     /gene="xseA"
                     /locus_tag="ACGVJG_001376"
     CDS             complement(7966..9303)
                     /gene="xseA"
                     /locus_tag="ACGVJG_001376"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFJ6557342.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(9304..10158)
                     /locus_tag="ACGVJG_001377"
     CDS             complement(9304..10158)
                     /locus_tag="ACGVJG_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFJ6557343.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(10255..10704)
                     /gene="nusB"
                     /locus_tag="ACGVJG_001378"
     CDS             complement(10255..10704)
                     /gene="nusB"
                     /locus_tag="ACGVJG_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFJ6557344.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(10697..11122)
                     /locus_tag="ACGVJG_001379"
     CDS             complement(10697..11122)
                     /locus_tag="ACGVJG_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFJ6557345.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(11368..12432)
                     /locus_tag="ACGVJG_001380"
     CDS             complement(11368..12432)
                     /locus_tag="ACGVJG_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFJ6557346.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(12604..12849)
                     /locus_tag="ACGVJG_001381"
     CDS             complement(12604..12849)
                     /locus_tag="ACGVJG_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFJ6557347.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(12988..13362)
                     /locus_tag="ACGVJG_001382"
     CDS             complement(12988..13362)
                     /locus_tag="ACGVJG_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFJ6557348.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(13445..14359)
                     /locus_tag="ACGVJG_001383"
     CDS             complement(13445..14359)
                     /locus_tag="ACGVJG_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFJ6557349.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(14380..15183)
                     /locus_tag="ACGVJG_001384"
     CDS             complement(14380..15183)
                     /locus_tag="ACGVJG_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFJ6557350.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(15216..16205)
                     /locus_tag="ACGVJG_001385"
     CDS             complement(15216..16205)
                     /locus_tag="ACGVJG_001385"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFJ6557351.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSIGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(16308..16799)
                     /locus_tag="ACGVJG_001386"
     CDS             complement(16308..16799)
                     /locus_tag="ACGVJG_001386"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFJ6557352.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(16990..19797)
                     /locus_tag="ACGVJG_001387"
     CDS             complement(16990..19797)
                     /locus_tag="ACGVJG_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFJ6557353.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            20443..21237
                     /locus_tag="ACGVJG_001388"
     CDS             20443..21237
                     /locus_tag="ACGVJG_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HFJ6557354.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT         6208 a         4604 c         3523 g         7035 t
ORIGIN      
        1 tccataaacc gccgaatttt atttttaaga ctcatttact tcactttcct taaattgtcg
       61 tcattaatag acaatccttt ttcttctgcg atttgtttaa tccgttcagt acgagaaagt
      121 tcgttttttt cttgttctaa actcgttttt tcttgctttt tatcattgat ttctgtttgt
      181 attgacgtga tttctttttc agctccgctg atatttgtgc gcagcataac tgttaaaaca
      241 cataaagcaa caatagcaat cgccacgctt gccacagcaa ttttttccaa agcagaaatc
      301 tgctttaatc gttttgccgg tgattgtgga agaatgattt cttgattcga agaagaatca
      361 ggcatttgtt gttccggctg atccaccaat tcaggtaaat catattcgta atcttgcatt
      421 ttctttaatt ctgccatcat tctactcctc tttcattctt attttttctg caatacgtaa
      481 ctttgcactg cgtgatcgat tgttttctgt tagctcttcc tctgaaggaa ggattggttt
      541 acgagtaatg actttcagtt ctggttggaa ttcgtcaggt atgacaggaa gtcccggtgg
      601 tagatcttgt accgtactat attctttaaa catacttttg acaattctgt cttccaatga
      661 atggaatgtg attacgctga ttcttccatt gactttcaat aaccggattg cctgttctaa
      721 agaggcctct tcggcaccga gttcgtcatt tactgcaatc cgcacagctt gaaaaattct
      781 ttttgcagga tgcccgcctt ttcttcttgc gggagctggg atgacttgtt taatgatttc
      841 gacaagctca ccagttgttt ggattggttt tgtttttcgt actcgttcga tttcacgagc
      901 aatttgtttg gaaaatttct cctcgccata gcggaagaaa atctttacta attcatggta
      961 ggaatattca ttaattacgt gataagcgga taaaggagct gactgatcca tgcgcatatc
     1021 caacggtgca tcttgatgat aactaaatcc acgttctgct tcatctagct gaggagagga
     1081 aacccctaga tcatacaaaa tgccgtctac cttatctaca tgctcactca agcgttcttc
     1141 taattctctg aagttcgctt tgataaatgt gaccatacct tgttcgacat aagaagctaa
     1201 acgcagcttg gcgtgatcga ttgctttttg atcttggtca aacgcataca gatgcccttt
     1261 agaacttagt tggcttaata aatattcact atgtccggca ccgccgagtg tacagtctac
     1321 ataaacgcca tccggtttga tgttcaatcc atctaccgtt tctttcaaca tgactgtata
     1381 gtgttgaaat gtttcagtca ttagtcagcc tcttcctatt cttatagtcc gaaatcaatc
     1441 atactttcag caatttcgtc aaagttctct tctgctgctg cagaaaaatc ttgccatcgt
     1501 gtctcatccc aaatttcaat tcggttagat acaccgatga ctacgcaacc ttttgtcaaa
     1561 gctgcataat ttcttaacgt gctgggtata ttgattcgtc cttgtttatc aagttcgcat
     1621 tcagtggcag ccgaatagaa gaatcgcaca aaagtacgtg cgtctttttt agccaacggc
     1681 atttcgttca gtttagcttc cagattttcc cattcgctca tcggatagcc gaaaagacag
     1741 ccatccaatc cgcgtgtcag aacaaacttt tcgccaagtt cttcacgaag ttttgagggc
     1801 acgattaaac gtccttttgc atcaatatta tgttggaatt cacccatcaa cattgaaagc
     1861 cccccattcc tccactaact aaaattagtc taccacaatc ccccactttc taccaccttt
     1921 tctgggaaat ccgggaacaa ctttttttat tcataaacat ttttttaaaa atcaagctgc
     1981 aaaaagcctt ttaaaagact ttttacagct tttctatttt tttcataggt aagaaagtat
     2041 gttcacaatg atcaagacag cgtacaagac gattgtcaac agaaaaacca aacgccaagt
     2101 catcttgaaa taacgcggat aaataatttc ttcataataa tatgcttgga aaacagcaat
     2161 acaaattccc attagcaaaa cagaaataaa gaaaaacggt aagatagaaa ttgtccctgt
     2221 atttctagac aatgcatgca taccgcccca caaaaaagga atcgccaaat caggggcttt
     2281 tattttaaaa cgacgattta acgaaaataa ttttacaaga aattggcaac caaacaaaac
     2341 gattaccggg taaatatacc aaaacaagat aagtggtgtg aatgtgccca tgaacgtaaa
     2401 ctcctttttt atcgttgctt caagtatact gtatatactt tacacaactg tttcatttta
     2461 tttaaattta tgcagttata ttctatatct tacaaacaca ttccatgtat tccaattccc
     2521 tatccgttat aataaaagaa acaggcaaca ggaggaaaat catgatcaaa tattttacat
     2581 tagataatga gaaactgcgt aataccggaa aggaaactga ccaaacgatc tggtattgtg
     2641 tagaacgtcc taatcaagaa gagattcatc agctgacaca tcaatttgaa attccgtcag
     2701 attacatcac ttccatattg gacgatgcag aaaattcccg tacagaagaa ttcaatcaat
     2761 caaagttgac aaagccagcg ttattattgt tacaatatcc ttttgctaaa acaagtccta
     2821 gcggttattt tcaagtggat acatacccgc tctctattat tttgacgccc aaaaagaagc
     2881 ttatcacaat tacgaatcat gaaccactgt ttttaaagaa agtctttagc cagacctttc
     2941 caaaaagtga tatttcgcct aatctcaata tcttctttca attcatttgg caggtcacac
     3001 tctcttacaa cagttattta gctacgcttc aatctcaatg cgatactttg gaagccaaac
     3061 ttcaagtatc cactgaaaac aaacaacttt atcaaatcat ggatatccaa aaaagcctcg
     3121 tttatttcag agaagcgact tctgctaatt tagatgcatt gaaagaacta gcgcaaaata
     3181 aacttttcca agaaaatcat gctttgaaaa atcatttacg tgatgtattg gtggaaacag
     3241 agcaggcaat gactacttct cgcattcaat taaaactagt agaacaaatg aatcagactt
     3301 tttcagcaat cgtatcgaat aatctgaata atattatgaa aattttaact tctttaacga
     3361 ttattttaac tataccgaca atcgtcggca gtatctatgg aatgaacatt aagttaccta
     3421 ttgctgaaag ggatgatgcc tttatatggc tagttctatt aacattattt atttgtatcg
     3481 taactaccta ttatttaaaa aaagggaagt ttttataaaa aagccctctt gtggaaatgg
     3541 aaaaaagatt gtacacttga atcaactaat taatgacgag gttgaaaaca atgcgtgaaa
     3601 aaaaacaaac aagtactttt gctaaagtaa caaaatttgt catttggaca atgctgattt
     3661 taacgattgg atctgttgta ttgactgctg taatgagtgt catgtaaaaa taaaatatag
     3721 ctatcagctg ctgaaaagga cctgaaccac tgcgaggtgg ctcaggtcct ttttgcgagt
     3781 ttcaatagtt cttttgcatg ttcgagtgtg agttttgtaa tctcacttcc agccagcata
     3841 cgtgcaattt cttcttctct ttcttttgtt ttcaactctg aaacagaagt ttgcgtacgt
     3901 ccatcaatca ctgctttcac aatataatat tgatagtcgg cgactgcagc aacttgaggc
     3961 agatgagtga tgcacaaaac ttgagagtat ttcgatattt gagaaatttt atctgcgatg
     4021 gcctgtgcaa ctcttccgct cacacctgta tccacttcat caaaaataat acttgtcaca
     4081 ccttgctcag aagaaaaaat agttttcaaa gcaagcaaca tccgagacag ttcgccacca
     4141 gaagcgactt tcaccaaagg cttcagtggc tctcctggat tggtcgtaat gtaaaattcg
     4201 atttggtcaa atccatcatg attcaattgt ctattttctt cttttaagaa acgtacctca
     4261 aactcagtgt tttccatata cagacttttc aattcatgca gaatggattt ttccaattct
     4321 tttgcgattt ttttacgcgc ttcatgaaga tctgcggctt gttgctgaag tagaagctct
     4381 ttttgctcta gttcctcttt cattttgtcc aattgacctt ctgaaaaatc agaagaagct
     4441 agctcttcgg tgatttcgtc atagtaagcc aatattgact caatggattc gccgtattta
     4501 cgtttcaact gtcggattaa ttctaaacgt tgcgtaactt cttctaaacg actctcatct
     4561 aactccaata aatcaatctg acgactcata tcgccgattg catcttgcag caaatagtaa
     4621 gcactttgga tattttcata aattgcttca tactccacat ctagatgggc gatcccttgc
     4681 agttctgaaa cggccaagcc aacaccatct acactgtttt gttcattatt cgtcagtgct
     4741 ccgtaacctg cagctaatcc atcagcaatt ttttgataat tgctaagttt ttctctttct
     4801 tcttttaatt tctcttcttc tccgacttgc aaatcagcag cagctatttc ttcttgctgg
     4861 aaatggagca tatctattct ctgaacatag gatttttcat tttgctgcac ttttcgtacc
     4921 cttttttcca gctcacgata agagatatat tcttgctgat aactcttttt cttctgttgg
     4981 aaagctacat ctccaaaacg atccaataaa gcaagatgtg attcaggctg gagcagttcc
     5041 tgatgttcgt tttgtccttg gatatctact aaataactgc cgattcttct cagattagct
     5101 aatgtgataa tatgaccatt cactcgacaa acattttttc cagttaagga catatcccgt
     5161 cgaacaatca gattgtcctc atctgtttcg atccctaatt caaccatcag ttcagagaat
     5221 ccttcttgtt ttggtagttc aaacaaccct tcaagcacac atttttctgc tccttgacgt
     5281 atataatcgc ttgaacctct tcctcctgct aacagaccta aagcatcgat aataatggat
     5341 ttacccgcac cagtctctcc ggtcagcgcg gtcattcctt cgtgaaaaga aagccgcaat
     5401 tccggaataa tcgcaaagtt agtaatactg atttcttgta gcatcacatt cgctcctcaa
     5461 cttaaaggta atgcaggaag tctttcttca ataaaactgc ctggtcttca gttcgtgtaa
     5521 tcatcaaaac attatcgtca tcattgataa tcgaaaataa ttctttcttg taacgtttat
     5581 caatcaaatt tgctgctgcc gaagcatttc ccggcaatgt tttcaaaata acaaattttt
     5641 ccatttggtc aactgccacg aaagcgtctt ttaatagttt ttccaattta gtatttacat
     5701 cttcgtttgt ttcagctggt atgctataac ggtagccgcc atctgaagcc ggtaccttga
     5761 tcagtttcag ttcctttata tctctggaaa tcgtagcttg agtaacagat attcctttgc
     5821 ttttcaggat ttcaacaaat tcttcctgct ttcggatatc ttgctcactc aacaatcgag
     5881 taatcaaacg atgccgatct ttttttctca ttacgtcttc gctccttagc atattcttct
     5941 taatcaatga tagcttattt ccttggtata ataaagaaga atccgcatac ttctcacttg
     6001 attttcacag ttacaattta tttatggtca aactgttcgt gtgcatttga aaccacttct
     6061 gccatattaa tagctgaatt gattgttccg ctttcgggta ctttcctcaa gtgagccaag
     6121 aactcgatat ttccttctcc ccccgtgatc ggagaataac ttaattccag cacatcatat
     6181 ccaccttgtg ccgcaaatga caaaatgtct tctaaaactt tttgatgggt ctgagggtca
     6241 cgaacaattc cgtttttccc tacagcttct ctgcctgctt cgaattgggg tttgattaaa
     6301 gcaatcactt ctccctcttc ttttaaaata gcatgtaatg gaggcagcat cagttttagt
     6361 gaaataaaag aaacatcgat aacagcaact tctggaattc ctaaggtaaa gtcttcgggt
     6421 tttgcataac gaaaattcac tctttccatt acgacgactc taggatcttg tcttattttc
     6481 caagctagct gattgtagcc aacgtctaaa gcataactca tttttgcgcc attttgcaaa
     6541 gctgcatcag taaatccgcc agtggatgca ccaatatcta acaatgtttt tccttcaaca
     6601 gatagatcaa atgctttgag tgctttttct aattttaatc cccctcgaga aacataagga
     6661 agttttttac ctttgactct taattcacta ctcactggaa ttttttctcc cggtttatca
     6721 aaccgctcgt ttttttcgtt gtaaatcaaa ccagccatca ctgaacgttt ggcttgttct
     6781 cttgtttcaa ataatccttg tttgaccgct aatacatcta cgcgttcttt ttccattctt
     6841 tccctcattt ctccacatca aattgcttga tcatctggcg catcagcccg ccatcaaaag
     6901 aaaaaacgtc ctcttctaat ttgtctatga tcttgttggc ggataacagc tcaatctcca
     6961 acgcctcacg tgtcttttct aggcctaata aacgcgggta agtatttttt tccatacgtt
     7021 catctttacc agctgttttc cctaatgttt tcgttgtacc aatcacatcg agtagatcat
     7081 ctcttatctg aaaagctaaa cctaaatgtt cggctaatcg ttgaagtagc aataagattt
     7141 cttcaggttg cttcgctaat atgccaccag ccagcaaggc ataacgaatc aaatgccctg
     7201 tctttctttc atgaataaat gctaattctt ctaaagaaag ctttttcgat tcgccctcga
     7261 tatctgcagc ttgcccagca accatacctt gtgatcccgc acaaacagct aattgctgaa
     7321 gcagcagaag cttaggagaa ttacctaagt gtgccatact gattagctgg aatgctcctg
     7381 taagcaaacc atctccggcc aatatagcta atgcttcgcc gaatacttta tgattggtag
     7441 gttttcctct acgaagatca tcattgtcca tcgcaggcaa atcatcgtgg atcaaggaat
     7501 acgtatgcac catttctaaa gcagctgcta cttgatagac gggaacatca ataggctcat
     7561 caaaggaagc aacggccgtt aaaagtaata agggacgtat tcttttcccg cctgctttca
     7621 ctgaataact catcgcatca tagagtcctc tatccgttgt atgctcatct aaaaaagaca
     7681 agatttcttt ttctacagca ggtagatgaa tagctgaaaa gtcagttaat ctcatctcag
     7741 ctttcctcgt ttccgtcaaa atcaacttct tcatttgatt ctgtcatcat tttcgtcaag
     7801 gtctcttctg ctttagctaa tgtatcttgg caatgtttgc ttaattccat tcctcgttta
     7861 aacgaatcta gagctgattc taatggaacg tctccttgtt ctaactgcat gacaattttt
     7921 tctaattctt gcagtgactc ttcaaaagtt ggattttcca tatttttatt ctcctttgtc
     7981 tatctcttgt acttgtcctt taacgattcc atcttgataa tggatcgtca gcaaatcccc
     8041 ttgatgaatc tcggtgacac tttttactac ttgatcattt aacgtcgtat agctgtagcc
     8101 tcttcccatg attttcaatg gactcaacaa atcaagagat tgaacagctt gagcaaaacg
     8161 ctgctgctgt tgtttcatat attgttggat tttctcttct aagcgttttt ctagataagc
     8221 agtttcttgg cgagctgctt taactcgata aagcggagcc acttgttcca attggtggct
     8281 taatgcgatc gtttgttttt ctttcgcatg ataaatttgc tgtgtagctt gatataatcg
     8341 ctgtttcaac tggtctaatt tgatggtctg accttcatac aatctttcag gctgccgaaa
     8401 tacataggaa cgtcgagcac gctcgaaccg ttcttgtttg cgctggatct ggcgtacaaa
     8461 cgcctgctgc aatctagctt gccgttcttg aatgcgcatc aactcttctg acaaaacagg
     8521 aacagctaat tctgcagctg ctgttggagt ggcagcccga acatctgcta ctaaatcagc
     8581 gatcgtggta tctgtttcat gtcctacaga tgaaatcacc ggcgtattag cttgataaat
     8641 cgcccgagca acccgctctt cattaaacgg ccataaatcc tcaatcgaac cacccccacg
     8701 accgatgatc attgtatcga aattgcccat ctcttctact ctctggatat tcctgacaac
     8761 attgtccgct gcttggtttc cttgtacaac tgttgggaac aaaaccaatt ggacaattgg
     8821 atatcttctt ttcaccgtcg tgatgatatc gcggataacc gctccgcttg gactggtaag
     8881 gaccgcgatt cgctttggga aacgtggcag cattttttta ggcccttgaa aaaggccttc
     8941 cttcatcagc ttttcttttc gttcttccaa ttcttgatat aaagcaccta ttccatcagg
     9001 ctccatgtgc tctacataga tttgataaga acctcccgct tcatataatg aaatacgccc
     9061 aacgacaagg actttcattc cttcttttgg ctggaatcgt aatttttgga atgctccttt
     9121 gaacataata gcagatattt ttgcatggtc atcttttaag ctgaaatatt gatgggcatt
     9181 ggcacgtaaa cggaaattcg atatttcgcc tgttaaatat acgcgttcta aataaggatc
     9241 ggcatcaaat tttcttttca aatacttcgt taatgtagtg acagttaaat attcttgtgc
     9301 catctattca cctgctccaa aagcttcggc agattttacg gtttgataca tcagcatcgt
     9361 gatagtcatt ggccctacac cctttggcac aggagtgatc cagcctgcaa taggctccac
     9421 ttcatcaaat ttcacatcac cgatcagctt accgttagca tcacgattca tccctacatc
     9481 tatcactaca gcacctggtt ttacgaattc ttttgtgaca aaatgtccac gtcctattgc
     9541 tacgactaaa atatctgctt cttgtgccag ttcagccaga ttttcggttt tagaatgagc
     9601 aacagtaaca gttgcatctg ccatcaagag tagctgagcc atcggtttac cgacgatatt
     9661 gcttcgtccg ataactactg ctcgtttacc cgccggatcg atagaataag cttcaaacat
     9721 tttcatgatg ccgtatggag tacaaggaat cttgtccggt ttacccgcaa ggagtctgcc
     9781 taaattcatc ggatgaaaac cgtctacgtc tttttcaggg cggatagcta acaggatctt
     9841 ttcttcatct atatgctttg gcaatggtaa ttggaccaat ataccatgaa aactaggatc
     9901 ttgattgtat ttttcaattt ctgcaagtag ttcgtcttca gaaatcgtct caggataacg
     9961 ctctaccaat gaatggattc cgatttcttt tgctacacgt tctttatttc ttacatagat
    10021 ttggctggct ggattttccc ctaccaataa aacgactaat ccgggatgga taccattatc
    10081 tttcaatttt tggatttttt cagcaatctc tgcctgcatt ttttcagcta attcttttcc
    10141 gttaatcaat tctgccatct aaccactccc agatattttt tgtgttttca ttctaccata
    10201 gaattttccc gatttataac aggtttatga tgattcatag aaaaaagcta ggacttacgc
    10261 cccagcttct aattctttca atacattgga taaaacaccg ttgacgaatt tacgtgaacg
    10321 gtcatcacta aatgtttttg ccaactcgat tgcttcattt aaagcaacag ccgctggtac
    10381 atctgtcaca taagtcattt caaaaatcgc gatacgcaag atcaccaaat ccatttttga
    10441 gatccgattg atgtgccaat tgttttttaa atgtttttca atcactttat ccaattcatc
    10501 tttttttgca cagacaccgc caactagcgt atcaagataa acaggaacaa actctgattc
    10561 atcttcgttg atcatgtcgc ggtggtctaa ttcgattgcg ttgaaaatcg catcttcttt
    10621 tgttaggtct gcattgaagt ctaatggaaa taaagcctgc aatgccattt cccgaatctc
    10681 gtgtcttgat aattctttat tcattctctt cctcttcgtc ttcaaaaagt tcttcgattg
    10741 ttggttgttc taatttttca ggaacaacag ctactacatg gatattgact tcagataaat
    10801 cgatatccgt catgaaaagg acttgctggc tgacccgttc ctgcatttcc attgctactt
    10861 taggaactga aacaccatat tctagataac agtaaagatc tactttcaag ccatcttcct
    10921 cgtttgctaa gtaaacacct tttccgtgag ctgcacgtcc taataattca gtgacattgc
    10981 tggcaaaagt tccgcgcatt ccgtatacgc cttctacttt agaagctgca atcccgataa
    11041 ttacttcgat gacttcagga gcgatcacga tctctcctaa atcttggttt gcgtttaaaa
    11101 tcaaattttt ttcatctgcc atttatttat tcctcccttt gcctaagcac gagatacatc
    11161 cgaattctct atgatgcggc atagtgatca cgtggtaaga aaactgccgc cgatcagctg
    11221 caaactgcta tgaattggag aaaacaccca actttattat ctcttacagc ttaaaagttc
    11281 ttctatagtg tatcattaaa tcgagtgatt ttctataaca aagtctgtga acactgattt
    11341 ttacgcttac ttcttctatt gatgaaacta taagatgatc aattcttttg gtgaacgagt
    11401 caacacacga tttccatctt ctgtgatcaa caagtcatcc tcaatacgta ctccaccgat
    11461 tcctggtaag tagatccctg gttcatcagt gatcacattt cctggtacaa attgcttttc
    11521 tgcacgagaa gaaacgtttg gtccttcgtg gatctctaaa ccgatcccat gtcctgtaga
    11581 atgtccaaat gcttccccgt aaccatgttt tgaaatataa tctctagcaa ctgcatctaa
    11641 ttggacacca gtcattcctg gttttgcagc atcaataact gctaattgag catctaagac
    11701 tatttgataa atttctttta atttttcccc aggatcacca atagcgaatg tacgtgtcat
    11761 atcggaaacg tacccttcgt aatagcaacc aaagtccaat gtgatcaagt caccttgttc
    11821 gatgattttt ttgcttgcta caccatgcgg catagccgaa cgcaatccgc tagcaacgat
    11881 cgtatcaaaa gaaacacctg aagcgccaag agaacgcata taaaagtcta gttgatttgc
    11941 gacttcaatc tcagtcatgc ccggttggat cattttcagg atgtgatcat atgccatatc
    12001 cgcaatatga catgcttttt cgataatagc gatttcttct tcatctttca cttctctgag
    12061 ttcttcgatc atctgtgaaa caggtactaa ttccgcatcg atgatttctt ctaaaaccga
    12121 atattctaaa aatgagactg ttgtttcctc aaaagctaat gagtgcagtt cttctttgcg
    12181 tactaagttt gctacctcat caaaaatagg tccgacattt ttaataattt caaacccttg
    12241 agcctgcgct gccgcctgct cggtataacg aaaatcagta ataaaaaatg ctttttccaa
    12301 agtaataaca gctaatcctg tcgtacctgt aaagttcgtt aagtaacgaa gattgtacgg
    12361 actggtaacc aaaaatgaat ccaaattttc ttcttgcatt tttttgcgca atttttctac
    12421 acgtagcatc ataatgaaac tccctctttc tttgcacgct tttttccatt atagcaaact
    12481 tttcttcgta cacgacagtt aaataaaaat ttccggaaaa cttcttttta ttttaaataa
    12541 ataaagccaa aaaggagtca cagtctcgct gcaactcctt ttagtttact attttgtaaa
    12601 aacttatgat cgttttccaa agaaaaatga gacaactgct accaaaatca ctgcgcctaa
    12661 aatagatgga ataagtgcca tacctgcagc ttttggtccc cattcgccga aaatcgcttg
    12721 tccaatactt gaaccgacta gtccagcaat gatattcgtt acacagccca tcgaatttcc
    12781 tttactggtg atcgcacctg caattgctcc gatcacggct ccaacaatca atgtccataa
    12841 ccaactcata atataacctc cttctttgct aatcaatttt agcatatggg atttaaaggg
    12901 gacaaataat tggttttgct atttcatatt ttcttctgca aagtaaaaaa ataaagaaaa
    12961 cctcctctta gaagttttct ttatttttta cttgcgttta aacatactac gaataccacg
    13021 tttcactaca tcaaagaaac tcaatgattg taccattcta tcttcatcta catattctct
    13081 gatcgcacga agcacttttt taggttcttt ggaagaaaat gtgtatgtac catttttttt
    13141 cgtttgaatg gcatatcgag gaatccattt tccctttagc atgactgatg ctagtacata
    13201 atccacttct tcccatggaa tttggatata tttgcgtcta tctcgcgcat ggtaaaactc
    13261 aaaaccatta tcacctatca tgatttgccc gtaatctgtc agacccgtaa acgaagtagc
    13321 atcaattacc aagtctactt ttgtattgat tgattgaacc attttctccc tccagatatt
    13381 tgtttatgaa cagtatacta tataaaaaaa gctcgggaca acgcccgagc tatattttta
    13441 tggattataa aagtccgatg acgtgtccaa caacaccgac tacgaataaa ccaaggatga
    13501 taacgattgg agaaaccttt ttcttaagca accacataca taacaatgtt aatcctaatg
    13561 cagctaaacc aggaatcaat tggtctaagt tgtcttgtaa tgttgttacc ttttcaggag
    13621 aaagggctaa gccattatta acttgttcaa atgcactctt gattccttca ccacctgaag
    13681 gcaatttatc ccattcgatg taagcacctt tatctaattt tacagttgat acaactggta
    13741 agaatttgat tgatacccat ctttctacaa gggcagctaa tacgaacatc cctaaaattg
    13801 aagcaccttt tgttacgtct tgtaacaatc cgccagaaag gtcatcagta attttagaac
    13861 cagctttgta gccaaattct tgtgtatacc acatgaatgc ccaacgaata atgttccaac
    13921 ctaagaagaa gatgattggt ccaaggatat ttcctcccat agctaatgaa gcacctagtg
    13981 cacctagcat tggacgtact gtaaaccaga acactgggtc accaacacca gctaaaggac
    14041 ccatcatacc tactttaacc ccttgaatcg ctacatcatc aacaggcgcg ccattcgcac
    14101 gttcttcttc cagagccaat gtaaccccta aaattggaga agcaatgtat gggtgtgtat
    14161 taaagaattc caagtgacgt tttaatgctg cagaacgttc ttcttttgtt ttgtataatt
    14221 ttttgattgc tggaatcata gaaaatgccc agccaccgtt ttgcatacgt tcgtagttcc
    14281 atgaaccttg aatgaaggta gagcgccacg caacagctaa acgatctttt tttgataatt
    14341 tgattttttc ttctgccatt ttattttcct ccttcaagat taatagtcat tcaaaatgtc
    14401 gcctaaaggg tcaccagagt tgcttccgcc tccattattt gatgaaccac ccattttaga
    14461 aaggttcaag taaataagag ctaatgcaac acctaaagca cctagagcaa tcaatgttaa
    14521 ttgagaaatt gcagctacaa cgaaaccaat aacgaagaat ggccatactt cttttgtagc
    14581 catcatgttg attaccaaag cgtaacctac agctacgacc attcccccac cgatagccat
    14641 accatctgtt aaccatgctg gcattgattc aaggaatgat tgcacagttt gtgctgggat
    14701 aaatagcaac gctgctgctg ggaccgcgat acgaacccct tgtaaacata cagcaatgat
    14761 gtgccacatt tcaaccttac ggatgtttcc ttcttcagca gccgcatcca tcaaatgaac
    14821 gattggaaca gcgattgtac gtacgatcat tgttaagaac aaccctgcaa cagccaaagg
    14881 aacggcaatc gcgatcgcag aaggaacacc tttaacacct tgtccaccta aaactaaaat
    14941 aattgctgat gctactgatg ctaatgcagc atctggtgct acagcggcac cgatatttgc
    15001 ccaacctaaa gcgatcatct gaagcgtacc gccaagtacg ataccagctt cgaggttacc
    15061 ggtaactaaa ccgatcaatg tacatgccac tagtggttga tgaaattgaa attcatcaag
    15121 aataccttcc ataccagcta gaaaggcaac taatacgact aaaattattg aaataataga
    15181 catgataagc ctcctataaa ttttaatatt atttattacg cttttgctaa ttcagctttt
    15241 gcttttttca aaatatcatc catgttatcg cgtgaatcat taggtacttt acgtacatcg
    15301 aatttcacac ctttttgttc aagcttctca aacgcttcaa cgtcttcttg tcccatagac
    15361 aatactttac ttacaacgac tttaccgatc gaatgcgcca ttgacccaac atttaattct
    15421 ttgatgtcta cgccaccttc aacagctgtc aatacgtctt caggattttc aaataaaagc
    15481 aatgctttgg tatttccaaa acgagggtct tttgctactt cgatcatttt gctaatagga
    15541 ataacatttg cttttacacc tggaggagca gcttgttcga ttagtctctt acgcagatca
    15601 tcctttgata ctgcatctga aacaacgata atacggtttg gttgcactgc ttttgtccaa
    15661 gctgtcgcaa cttgtccatg taacagacgt gagtcaacac gtgctaatac atatttgatt
    15721 tttccgtctc cgaccacagt tccttctgga agtgctcctt taggttgtgc gtcttcaact
    15781 gctgctttcg gtgcttctgc cggttctaat tcttccggtt ttacttttac accgtctttt
    15841 gctgtttcaa tgatgtgtgc tgcaatttca tgagcagatt ccattgaaaa gcgtgatgcg
    15901 taggcttcaa tcaacattgg taaattcaaa ccgctgacga ttgcccattt atctttgtgc
    15961 tcttcgaata atgtgtttga ttgattgaac ggtgttccgc cccataaatc aactaagaat
    16021 aacacttcgt cttgattatc aaaagaagca accgcttctt ccaatttggc tctcaagtca
    16081 tctggacctt cacttggttt caaaatcacg gctttgacgt tttcttgttc gccaaagatc
    16141 attgaaccgg attgtaagat tccttcagca aattgtccat gacttgctag gataatccct
    16201 accatattta tacctcctat ttacttttgt tgtaacaaat tcaaaaatat taataattgc
    16261 taaccattac aacagaatta tttgaccttt tccaacaacc attcttgcta cacctttttg
    16321 attttcgcta gtagttccat caaaaggatt ttcctgtcag caggtacttt tcgaatctct
    16381 aactccactt cttgactgtt caagtaacga aaggaatcaa tatcttgttg atttactgaa
    16441 acaaaattgg taatcattga tttgccttca gtaaaactca taccgccgat attcaacgac
    16501 ttcaactgta caccgcagcg gacaaccgct tcaacatctt ggggattggt gaataaaagc
    16561 atcacttttg ctcgtgataa taatccacta tgatagattt gaatcatttt ctgcacggtt
    16621 attacatttg ctgtgatccc cggcggtgca gcttctttta gtagaaactt tctcaattta
    16681 tcttgagcga ctacatcgct gatcacaatg atacgttcaa cacctgtttg cttcgaccaa
    16741 actgttgcga cttgcccgtg gattaaacga tcatctatac gtgccaatcg aatatccatc
    16801 tgcaaaactc cttcttcctc actacttcac agaaacccct atcatttctg tgtgaacaac
    16861 cacctcctag cacacactac tataagcaaa aagcgtgcca acttttttac gatacactaa
    16921 ttgaatttaa aggattctta accaatacac aaaaacgata cactataaaa gtgtatcgtt
    16981 tttgtgtatt catggcacag tgtattcttt tttatctaac tgattatgga ctagacgtag
    17041 cagataatac atttcttcta tcggcacttt tacttttatc tgatcttcca acagaacaac
    17101 tgaggctgcc aattggctat aaagaggatc attcgttgta tttgtcaatt cgttttcttc
    17161 cacagtcaat ggttcattcc gaataatacg ttcgatcatt cctgctgtgt gcatcactag
    17221 gttgattttg aatccatatt cttcatcgcc gattcccgtc gtctgagaga tttgtgtaga
    17281 aaattgccac attggatcga ttaacttact accattaata aagatgaagt tatcagagat
    17341 aaactcaaca cacgtatttt tagctgactc ttcatctagg gagatgaatt cagtttcctc
    17401 caattctgat tccatcagca actgatccaa aatgacttcg atattttgat caataaaacg
    17461 ttctaaagga ataaatgggg cttggatatt caaatcagta atcccagtag tagctatgat
    17521 ctgatattct ttttggataa ctggcagttc ttctttcact tccacaatcg acaaagctag
    17581 aacttctaat tctgtttctt gacgcttgtt taccgctcgt tcgatcaatt ctttgattct
    17641 ttgtgctgta ccttcgccag atgcgcagat agccaaaaca gcttttttct tatgaatagg
    17701 ttttgcttcc tgaacagatt ctacagaaga tatttctgcg taacctcgaa aatttttcaa
    17761 tgattcatgc aacatatcta gatctgtccc taatactgag acctttcgta cagtttccaa
    17821 aactagagaa gttgttacca tatcaacagt acgtactgga attccagtgt ctctttgaat
    17881 ttgcgaatta aacgacgcaa gggaccccat atctactaag agtatgaccc cgcttccttc
    17941 atctacttct tgtacattgc gttcaattcg gtccaatgct gttttcggat ccatatccaa
    18001 aggcatgtct actgctcgta cgttctctgc atctaacaac tgttggacaa cttggaccat
    18061 actgcttgct gtgctgtttc cgtgagctgc aacgactaca ccgattctgc cgcttgcttg
    18121 atttgcccgt aacgagacga gtagaacggt caagtaatag tcttcacttt ctggaatctc
    18181 cacttgaaaa tacgtttcga tataactacg cacttctttc gcaacagcat attcatctgg
    18241 aaaatcaaaa gccatctcac ggatgttatc attcgtatgc cgttcttctc ctaagttgat
    18301 ttttttcaaa aaagaactga tatgcaagct catagcataa ataaagtttt gctgaaactc
    18361 atatggtaaa gatcttttga ccattacata aatctgattg gtcacttcta tgattttcgg
    18421 atctacaaat tctgctaatt tgctgtctgc attaaaagaa aaaccatggt ctttatagaa
    18481 agacttcaga tggatgttga tatctgtaga aatgaaatga ttgattgctt cttgatccaa
    18541 accatccgac ttcagtaaag ctgctttatc tccgatgata tcatatagat tataaggtaa
    18601 ctcataggag tccgattgga ttttcatgat cgtatcattt ggtgctacag ttatctttgg
    18661 ctccaatatt ttagaaagct ctgccattgc actgcggttg cttgctaatt gaatcaatcc
    18721 gctacgaatc ccttctgtca aatcatcgat tgtgatcgag atttcgggtg attgcatatg
    18781 atttagaaat cctcgtgcac atactaactg gatatttgac ttcaattgtc ctatatttcc
    18841 ataggtcaca ctgccgatca atgcttttgc tacatcttct gtcaaagaaa tctttcgctg
    18901 cgtgcggttt gcttcgtggg caatcatgat ttttactaaa tcgactttct catttgcagg
    18961 acgtttttca aaagccggaa gtttgatatt gattgggata cgccgaacaa aagtttctaa
    19021 tagagaagaa cctgggtctt ctgtggtcgc acctacgatc cgtacatttg cttcatggga
    19081 tttcgttgtt tccccaagac gactgtaagt tccatgatcc ataaagtaaa agatcatttc
    19141 ttgaccttca ggcggcaacc ggtgaatctc atccaaaaac agcataccgc catccgcttg
    19201 atcgataatc cccatttttt cttcatctgc gcctgtaaaa gcaccttttg cgtacccaaa
    19261 taaatgactc atcaaaagct cagggttatt ggcataatca gcacaattga aaacaatcag
    19321 ttccgtttcc tcatctatta catgatgcgc ttttgcaaaa tgatacatcg cgtgtgcaaa
    19381 ataagtcttt cctgatccgg taggtcctgt aatcaaacaa tttaatcctc ttggcggata
    19441 caaaatcgca gcttttgcct gttccactga atttttcatg ctgccgttag ctccaatgat
    19501 ttttgcaaag atatccttag tatctatttg gagtgtattt ttttcttcag agaccggcgt
    19561 ttctttgtaa ctgactacat gttttgtaac tggctttacg tgcgtaacga gtgtttgata
    19621 tatgtaacgt actggtcgtc cgtctgtctt tatcagtttt ccttcgcgca ctagctgatt
    19681 cagatcttta ctgctatttg ttcgctgaat ccctaacact tcagcaattt cctgtgttgt
    19741 tactccttga tcatattctg cctttgttaa atgagcggtt ttttctttga catacatata
    19801 aattcggtca atgcgttttg ccaatggtat cgctcctttt ctatagacta ttgtacttag
    19861 gcttcaaaac ataatactat gtatttattt ctgtttcgtc aactatttac ctgattttgt
    19921 tttgttgata acacgcggat agatagaaat gttcaattta aaagaacgaa aaatcccgat
    19981 acgattttta taatggaaac aagaaaaaga acactgttta ccatataatt atttcactga
    20041 tagcaaacaa tatagcttcc atcaattgaa aacgatagtt atcaaaacat cctgattcca
    20101 aattggcaaa aaaatattaa gtgtaaaaag acaaagtaaa cttcgtagcc tccactacaa
    20161 cttttatttt tataaacaaa aaaggctgtg acaaaagtct tgtcacaacc tctttaatca
    20221 taagattcaa ttaagcttct tgagctactg gataaacaga cacttcaatt atactgtttc
    20281 ctgataagct ctcaattctt ctgaacccat gatgttagtg cttttcttct attattaaat
    20341 atcttggaat tttctattct ttctaaaaag tatggtcaaa gtatggtcac tattgataga
    20401 atgttaatag tagtagaaag ctgatatcta ggaggaacta atgtgagtaa acaatgtgca
    20461 aagtgcggag gtaaaattgg cttaacttca tataaaatca aagataaaca aattgtttgc
    20521 ggcaattgta tgaaaaaagc tggttacggt atgactactc cttataaaat aataagatct
    20581 ttgaatttag aagatttaga taaggatcca tcagaaaaaa gaaaattaaa catatctgat
    20641 catactgcac aaaacaatca tgatataggt aatgatagaa tgagaaccac tgaagagatg
    20701 gtagaatttt gtcttaaata cggctacggt aaaggaatga ctaaaaaatg gacaactcat
    20761 cattttaact taatatcaga tcaacttaac agtgacgaat acgtaatctt ctgctttgta
    20821 ggattgcata attatatctc atcaactaag catgacaata attatgcata cgcattaact
    20881 aataaacgtt taattgtagc tcaacaaaaa atgataggca ataatgtaca aagtattata
    20941 ttagacaatt taaataatat tagtaaaaaa agaggagtgc tgttgggtac attaactatt
    21001 gatactcttg gtaaagtatt gaatgttgca gttgataaag atactgttga taggataagt
    21061 gattctttaa acgaaattat ttataattta aaaagaagaa atatttctgc tacaaattcc
    21121 aatacatcaa caatttcctc agcttcagaa ataagaaaat ataaagaact attggatgat
    21181 ggaatcatta cagaagaaga atttaataaa aagaaacaag aattattaga tttataatat
    21241 ctatcaaaga agatgggcat ttaatttagc tcatcttttt ctatttacaa tacaaacatt
    21301 tgttcgtata ctctttttga ggtgaacttt atatgttaat ggaaggaaaa acacaattat
    21361 ggtttaaatt
//
LOCUS       DAYLZO010000041        21086 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.042, whole genome shotgun sequence.
ACCESSION   DAYLZO010000041 DAYLZO010000000
VERSION     DAYLZO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.042"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            6..305
                     /locus_tag="ACGVJG_001389"
     CDS             6..305
                     /locus_tag="ACGVJG_001389"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFJ6557355.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACGVJG_001390"
     CDS             378..1739
                     /locus_tag="ACGVJG_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFJ6557356.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACGVJG_001391"
     CDS             1761..2021
                     /locus_tag="ACGVJG_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557357.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACGVJG_001392"
     CDS             2035..2787
                     /locus_tag="ACGVJG_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFJ6557358.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACGVJG_001393"
     CDS             complement(2881..3486)
                     /locus_tag="ACGVJG_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFJ6557359.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACGVJG_001394"
     CDS             complement(3539..4513)
                     /locus_tag="ACGVJG_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFJ6557360.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACGVJG_001395"
     CDS             complement(4891..5610)
                     /locus_tag="ACGVJG_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6557361.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACGVJG_001396"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACGVJG_001396"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFJ6557362.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACGVJG_001397"
     CDS             7161..8558
                     /locus_tag="ACGVJG_001397"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFJ6557363.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACGVJG_001398"
     CDS             8639..9079
                     /locus_tag="ACGVJG_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFJ6557364.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACGVJG_001399"
     CDS             9227..9553
                     /locus_tag="ACGVJG_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFJ6557365.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACGVJG_001400"
     CDS             9554..10303
                     /locus_tag="ACGVJG_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFJ6557366.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACGVJG_001401"
     CDS             complement(10383..10838)
                     /locus_tag="ACGVJG_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFJ6557367.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACGVJG_001402"
     CDS             complement(11265..11540)
                     /locus_tag="ACGVJG_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557368.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACGVJG_001403"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACGVJG_001403"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFJ6557369.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACGVJG_001404"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACGVJG_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFJ6557370.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACGVJG_001405"
     CDS             14770..17436
                     /locus_tag="ACGVJG_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFJ6557371.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACGVJG_001406"
     CDS             17800..18552
                     /locus_tag="ACGVJG_001406"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFJ6557372.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACGVJG_001407"
     CDS             complement(18574..19728)
                     /locus_tag="ACGVJG_001407"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFJ6557373.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..>21086
                     /locus_tag="ACGVJG_001408"
     CDS             19913..>21086
                     /locus_tag="ACGVJG_001408"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFJ6557374.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQT"
BASE COUNT         6742 a         3691 c         4361 g         6292 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacac
//
LOCUS       DAYLZO010000042        20942 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.043, whole genome shotgun sequence.
ACCESSION   DAYLZO010000042 DAYLZO010000000
VERSION     DAYLZO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.043"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            168..362
                     /locus_tag="ACGVJG_001409"
     CDS             168..362
                     /locus_tag="ACGVJG_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557375.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACGVJG_001410"
     CDS             631..930
                     /locus_tag="ACGVJG_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557376.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACGVJG_001411"
     CDS             complement(1027..1461)
                     /locus_tag="ACGVJG_001411"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6557377.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACGVJG_001412"
     CDS             complement(1693..2403)
                     /locus_tag="ACGVJG_001412"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFJ6557378.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACGVJG_001413"
     CDS             complement(2393..3253)
                     /locus_tag="ACGVJG_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFJ6557379.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACGVJG_001414"
     CDS             complement(3258..3896)
                     /locus_tag="ACGVJG_001414"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFJ6557380.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACGVJG_001415"
     CDS             complement(3911..4210)
                     /locus_tag="ACGVJG_001415"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFJ6557381.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACGVJG_001416"
     CDS             complement(4253..5737)
                     /locus_tag="ACGVJG_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFJ6557382.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACGVJG_001417"
     CDS             complement(5757..6218)
                     /locus_tag="ACGVJG_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFJ6557383.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACGVJG_001418"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACGVJG_001418"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFJ6557384.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACGVJG_001419"
     CDS             7521..8291
                     /locus_tag="ACGVJG_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFJ6557385.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACGVJG_001420"
     CDS             complement(8564..9391)
                     /locus_tag="ACGVJG_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557386.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="ACGVJG_001421"
     CDS             9959..11194
                     /locus_tag="ACGVJG_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFJ6557387.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="ACGVJG_001422"
     CDS             complement(12193..12453)
                     /locus_tag="ACGVJG_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFJ6557388.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="ACGVJG_001423"
     CDS             complement(12480..13781)
                     /locus_tag="ACGVJG_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFJ6557389.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="ACGVJG_001424"
     CDS             complement(14073..14531)
                     /locus_tag="ACGVJG_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFJ6557390.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="ACGVJG_001425"
     CDS             complement(14544..15875)
                     /locus_tag="ACGVJG_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFJ6557391.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="ACGVJG_001426"
     CDS             complement(15886..17328)
                     /locus_tag="ACGVJG_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFJ6557392.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="ACGVJG_001427"
     CDS             complement(17315..18079)
                     /locus_tag="ACGVJG_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFJ6557393.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="ACGVJG_001428"
     CDS             complement(18183..19058)
                     /locus_tag="ACGVJG_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFJ6557394.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="ACGVJG_001429"
     tRNA            complement(19313..19397)
                     /locus_tag="ACGVJG_001429"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="ACGVJG_001430"
     tRNA            complement(19415..19486)
                     /locus_tag="ACGVJG_001430"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="ACGVJG_001431"
     tRNA            complement(19496..19568)
                     /locus_tag="ACGVJG_001431"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="ACGVJG_001432"
     tRNA            complement(19630..19714)
                     /locus_tag="ACGVJG_001432"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="ACGVJG_001433"
     tRNA            complement(19735..19805)
                     /locus_tag="ACGVJG_001433"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="ACGVJG_001434"
     tRNA            complement(19851..19922)
                     /locus_tag="ACGVJG_001434"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="ACGVJG_001435"
     tRNA            complement(19934..20006)
                     /locus_tag="ACGVJG_001435"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="ACGVJG_001436"
     tRNA            complement(20009..20082)
                     /locus_tag="ACGVJG_001436"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="ACGVJG_001437"
     tRNA            complement(20089..20169)
                     /locus_tag="ACGVJG_001437"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="ACGVJG_001438"
     tRNA            complement(20171..20246)
                     /locus_tag="ACGVJG_001438"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="ACGVJG_001439"
     tRNA            complement(20282..20354)
                     /locus_tag="ACGVJG_001439"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="ACGVJG_001440"
     tRNA            complement(20364..20436)
                     /locus_tag="ACGVJG_001440"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="ACGVJG_001441"
     tRNA            complement(20452..20523)
                     /locus_tag="ACGVJG_001441"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="ACGVJG_001442"
     tRNA            complement(20538..20627)
                     /locus_tag="ACGVJG_001442"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
     gene            complement(20667..20740)
                     /locus_tag="ACGVJG_001443"
     tRNA            complement(20667..20740)
                     /locus_tag="ACGVJG_001443"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20704..20706),aa:Asn,seq:gtt)
     gene            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="ACGVJG_001444"
     rRNA            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="ACGVJG_001444"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6603 a         4390 c         3331 g         6618 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
    20701 attaacagtc atgcgctact accaactgag ctatgccgga ataacatcag cgtggcgacg
    20761 tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct taacttctgt
    20821 gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg gtgttatctt
    20881 ttattgagta aattttgttc actcaaaact ggatttgaag tcacataagt ttttttccga
    20941 gt
//
LOCUS       DAYLZO010000043        20826 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.044, whole genome shotgun sequence.
ACCESSION   DAYLZO010000043 DAYLZO010000000
VERSION     DAYLZO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.044"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            29..1297
                     /gene="dltD"
                     /locus_tag="ACGVJG_001445"
     CDS             29..1297
                     /gene="dltD"
                     /locus_tag="ACGVJG_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFJ6557395.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            1438..2520
                     /locus_tag="ACGVJG_001446"
     CDS             1438..2520
                     /locus_tag="ACGVJG_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFJ6557396.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            2520..3323
                     /locus_tag="ACGVJG_001447"
     CDS             2520..3323
                     /locus_tag="ACGVJG_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFJ6557397.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            3393..4133
                     /locus_tag="ACGVJG_001448"
     CDS             3393..4133
                     /locus_tag="ACGVJG_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFJ6557398.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            4144..5148
                     /locus_tag="ACGVJG_001449"
     CDS             4144..5148
                     /locus_tag="ACGVJG_001449"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFJ6557399.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(5193..7037)
                     /locus_tag="ACGVJG_001450"
     CDS             complement(5193..7037)
                     /locus_tag="ACGVJG_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557400.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            7249..7767
                     /locus_tag="ACGVJG_001451"
     CDS             7249..7767
                     /locus_tag="ACGVJG_001451"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFJ6557401.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            7895..8578
                     /locus_tag="ACGVJG_001452"
     CDS             7895..8578
                     /locus_tag="ACGVJG_001452"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFJ6557402.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            8887..8960
                     /locus_tag="ACGVJG_001453"
     tRNA            8887..8960
                     /locus_tag="ACGVJG_001453"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8921..8923,aa:Arg,seq:tct)
     gene            9119..9478
                     /locus_tag="ACGVJG_001454"
     CDS             9119..9478
                     /locus_tag="ACGVJG_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFJ6557403.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            9601..11232
                     /locus_tag="ACGVJG_001455"
     CDS             9601..11232
                     /locus_tag="ACGVJG_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFJ6557404.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(11294..11935)
                     /locus_tag="ACGVJG_001456"
     CDS             complement(11294..11935)
                     /locus_tag="ACGVJG_001456"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFJ6557405.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    12121..12197
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            12217..12525
                     /gene="rplU"
                     /locus_tag="ACGVJG_001457"
     CDS             12217..12525
                     /gene="rplU"
                     /locus_tag="ACGVJG_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFJ6557406.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            12552..12896
                     /locus_tag="ACGVJG_001458"
     CDS             12552..12896
                     /locus_tag="ACGVJG_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFJ6557407.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            12909..13202
                     /gene="rpmA"
                     /locus_tag="ACGVJG_001459"
     CDS             12909..13202
                     /gene="rpmA"
                     /locus_tag="ACGVJG_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFJ6557408.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(13295..14443)
                     /locus_tag="ACGVJG_001460"
     CDS             complement(13295..14443)
                     /locus_tag="ACGVJG_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6557409.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(14455..14766)
                     /locus_tag="ACGVJG_001461"
     CDS             complement(14455..14766)
                     /locus_tag="ACGVJG_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557410.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(14819..15214)
                     /locus_tag="ACGVJG_001462"
     CDS             complement(14819..15214)
                     /locus_tag="ACGVJG_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFJ6557411.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(15250..15594)
                     /locus_tag="ACGVJG_001463"
     CDS             complement(15250..15594)
                     /locus_tag="ACGVJG_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6557412.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            15895..16116
                     /locus_tag="ACGVJG_001464"
     CDS             15895..16116
                     /locus_tag="ACGVJG_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557413.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            16243..17019
                     /locus_tag="ACGVJG_001465"
     CDS             16243..17019
                     /locus_tag="ACGVJG_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFJ6557414.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            17022..17210
                     /locus_tag="ACGVJG_001466"
     CDS             17022..17210
                     /locus_tag="ACGVJG_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFJ6557415.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            17212..17496
                     /locus_tag="ACGVJG_001467"
     CDS             17212..17496
                     /locus_tag="ACGVJG_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557416.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            17517..17705
                     /locus_tag="ACGVJG_001468"
     CDS             17517..17705
                     /locus_tag="ACGVJG_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557417.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            17844..17972
                     /locus_tag="ACGVJG_001469"
     CDS             17844..17972
                     /locus_tag="ACGVJG_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557418.1"
                     /translation="MAREEALKIGKVIADNWWANSRSIILSKQHIEKKKAWQQIKK"
     gene            18031..18270
                     /locus_tag="ACGVJG_001470"
     CDS             18031..18270
                     /locus_tag="ACGVJG_001470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557419.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            18276..18962
                     /locus_tag="ACGVJG_001471"
     CDS             18276..18962
                     /locus_tag="ACGVJG_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFJ6557420.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            18965..19795
                     /locus_tag="ACGVJG_001472"
     CDS             18965..19795
                     /locus_tag="ACGVJG_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFJ6557421.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            19812..20663
                     /locus_tag="ACGVJG_001473"
     CDS             19812..20663
                     /locus_tag="ACGVJG_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6557422.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            20660..>20826
                     /locus_tag="ACGVJG_001474"
     CDS             20660..>20826
                     /locus_tag="ACGVJG_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFJ6557423.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDG"
BASE COUNT         6908 a         3413 c         4106 g         6399 t
ORIGIN      
        1 aacaagcaac tgcattgaag ggttgagtat gaaaaagaaa ctttttgcta ttttcggccc
       61 ggttgtttta gctgttggct tactgtttgt gttttttacc tcctcctatc aagtagatat
      121 ctctaatcca gaaacgatta gacgtgcctc ttcttccatg tcagtgaacg tgttgaaagg
      181 agatgcgata aaaaattatg cattatctga aaaacaatat attccttttt ttggctcaag
      241 tgaattaagc agaatcagtc catttcatcc gtctgttctg gcagaaaaat accagcgaaa
      301 ctatcgtcca tttttgctgg gagcacccgg gacacaatcg ttaagccaat atatgatgat
      361 gcgttctgca ggagatgcta tgaaaaacaa gaaagtcgtc tttattattt ctcctcagtg
      421 gttcgttaaa aatggtgtaa aaactgatta ctttaatacg tattattctg aacttcaaac
      481 atacgactgg ttgttttcaa tgaagaaagt aacgcctgct gatcgctatt tagctagaag
      541 attattaact ttctcaaaag taaaagagaa tgatacgttg acggcgattc tgcaaacgat
      601 caaaaaaggg aagctacctt tacctgaatc tttgaatcag ctgcgcagcc aatggaacat
      661 gctgaagaga gaggatgaag tattcagcaa tattgggttg aaagatcgac agcaaaaaat
      721 cgatcatgag tcaaaacgat taccaaaaca ataccaagaa acagaattat ccattttagc
      781 aaatcaaatt ggagaacgag aaacgacgaa taatccattc gggttgaaaa acgattttta
      841 tacgcatcgt atccgagccc acgagccaga gctcaaacag tcacaaaaaa attgggatta
      901 tcgtttttcg ccagaatttt ctgattttca gctggtcttg gatcaattag caaaaaacca
      961 taacgaagta ttgttcatca tcccaccggt taacgagaaa tggagcgatt ataccggact
     1021 atctcaagaa atgttacaag gatttgccaa aaagatcaaa tttcaattaa atagccaagg
     1081 attcaatcga atagctgatt ttgtcaatca agcgggtacc aactatttta tggaagacac
     1141 gatccatcta ggctggaaag gctggttggc tgcagatcaa cagatcagac cgtttttaga
     1201 agaaaatcat attactgcta gtaagtatca tcttgacgat gcttttttta gcaaatcttg
     1261 gcaacatcaa ataccagata aactacaatt aaaataaaga aaacaggcga gtgttgctcg
     1321 cttgttttct ttatttttga atattctgaa taaaagataa aaataccaag tttgcctgaa
     1381 caaaaatcat aagcaatggt ataatcacaa tatgaatata gaaatggggg aaaacatttg
     1441 gatacaaaag aagaacagct acttattgat ttaacttctg caaaaggagt acctggaaat
     1501 gaagaagagg taagagaagt attcagagaa tatgccaaac cattcgctga tgatattttt
     1561 tatgacggtt taggcagtgt gattgccaaa catggtgctg gcggaggccc caaggtcttt
     1621 atttctggac atatggacga agttggtttt atggtaacaa aaataaccga aaaaggattc
     1681 ttggaattcc aaactcttgg aggttggtgg gggcaagtca tgcttgctca gcaagttgaa
     1741 atcaaaacac gtgaagggaa aatcgtccac ggtgtgattg gttccaaacc accacatgtt
     1801 ttaacacctc aagtaagaaa taaaccttat gaaataaagg atatgttcat tgatattggc
     1861 gcatcaagcc aagaagaagc caaagagtgg ggcatacgtc ctggagatat ggtcactcca
     1921 tatattgaat acaaacgaat gaatggcagt aaatatttac tggcaaaagc atgggataat
     1981 cgtatcggga cagctgtttc tctgcgagta ttagagaatc tatctaaaga agcacaccca
     2041 aatgttttat tcgcaggaag cgatgtgcaa gaagaagtag gcttgcgagg tgctcgaaca
     2101 agtacacacc ttgtaaatcc cgatattgct tttgctttag atactggtac agcaggagac
     2161 actccaggaa tgacaccaaa agaagcagat tcgattttag gaaaaggccc acagattttg
     2221 atttttgatg catctatgat cccgcataaa aaattattga attttgttat tgatgtagca
     2281 gaggaaatgg atattccatt ccaatataca gtgatttctg gcggaggaac tgatgcagga
     2341 caaatgcatt tgacaagaga tggtgttccg tctgtagcca ttaccgttcc tgttcgttat
     2401 ctgcattccc atacatcagt gattcatgaa gatgattatt tgaatacagt gaaactagtc
     2461 acagaattag tgaaacggct tgatagtgaa acagttgaaa aaattcggag ttatgaataa
     2521 tgaataatag aatacctatt tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc
     2581 aatggggaat caatacttgg cttgttttct tatctataat atttacgagc gtcatccaga
     2641 acttttttga cggtttcagt ccttttccgg aagaaagcat gcagcagcac ttattgagtt
     2701 ttctagtcga taatttactt ttattcgcat tcaactatgc attgttttac attgcgctag
     2761 tagttgtacg gggtggcaga ccaaaaagta accttttatt ttcagttttc caaagaaagt
     2821 attttggttc gatacttggc atcaatttga tcaattcagt catcaactgg ctactcaaca
     2881 gtttaatctt tctgcccatg tttttaatca gcggagtgag tacttatatg cagctgcttt
     2941 tcagttcaaa tgcggtacct acgggtgtca tgccagaaga ttttcttgat atcagctttg
     3001 ttttatcgat gatagggatg atttttgttt cagcactcat catgagtatt gttggcggtt
     3061 tattccaatt tgctgcatgg acaaagttcg actatcctga tttgtcggtt attcaatgtt
     3121 tgaaatatgg ctggttttta ttgaaagata gaattggtac gtatattttg ttgcagctgt
     3181 cttttatcgg ctggtatttg ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca
     3241 ttgtttatgt gaatgtgtca gttgcagaat tttatgaaca agcacggatt gaaaaaggag
     3301 gtccagacgc ctattttact tagcatgtaa gcagtttatt catcaaaaaa ataacttttg
     3361 tggtaggata aatctttgag ggggaatgta cgatgaaatt aactgtttta ggatgcttag
     3421 gagcgtatcc ttataaaaat caagggacaa caggttattt gcttcaatct gacgggttca
     3481 atttgttgat cgatgcaggt agtgctacgc tgatcaaatt acaagaacac cttgatccac
     3541 ttgatctaga tgctgtgatc cttagccatt atcatcatga tcatattgct gaccttggtg
     3601 tattacagta ttactggcaa cttcatccta agagaaatcc ggagacattg ctttcaattt
     3661 atggacacac aaaagattcc attcactttg aagaattgac gttggaaaat gtctcaaaag
     3721 gacatcctta ttttgaagct gaagaattaa aaattggtcc ttttctgatt acttttatgg
     3781 aaacaatcca tccagtagta tgttacgcca tgcgtatcgt agaagaaaca acggggaaag
     3841 tattcgtatt tacaggtgat tcaggttatt tggaaagttt cgctgatttt gcgaaagatg
     3901 ctgatttgtt tctagcagat acctatcttt ttgaaggaaa tgaacatcat catgcccatt
     3961 tcacttcaaa agaagctgga gaacttgcga aatcagctaa cgtaaagaaa cttgtactta
     4021 cacacctacc tcagtttggt gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag
     4081 gcaaagggat tgatatagaa cttgctgcag ttgacaaagt gtttaacatt taggaggaag
     4141 aacatgattt ttgtaccaaa tgaaaaccac gacccaagaa tcaatttagc aattgaacaa
     4201 tatttgctgc aagaaatgcc tttagatgaa cctattttgc tcttttatat caatgaacct
     4261 tcaatcatca tcggcagaaa ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa
     4321 gaaaatggca ttcatgttgt ccgtcgctta agtggaggcg gagctgtcta ccatgatttt
     4381 ggaaacctga actttagttt tatcatgcct gatgatgggg attcattccg tgactttgcg
     4441 aaagtaacac agccaatcat ccaagcactt catgaattgg gtgttgctgg ggcggaattg
     4501 aaaggccgaa atgatctagt cattgatggc atgaaatttt ctggcaacgc gatgtatgct
     4561 accaacggtc ggatgtttgc tcatggaaca attatgttcg actctgatat caatgaagta
     4621 gtcaatgcat taaaagtaaa aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt
     4681 tctcgtgtaa cgaatatcaa accatttctt ccagaagaaa agcaaaaaat gacaacagaa
     4741 gaatttcgtc aagaaatctt gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa
     4801 acgtacgaat tgactgatga agattggaaa aaaatcaatc gaatctctga ggaatattat
     4861 cgaaattggg attggaatta cgggaagtct ccagatttca atttctctcg ccaaaaacgt
     4921 ttttctatcg gttcgattga agtccatttg aatgtctcag aaggaatgat caaagaagca
     4981 aaaatctttg gcgatttctt cggactagga gatatctctg acgtggaaaa aaaattggtt
     5041 ggacagaaat atgataaagc atctctatca aaaatagtca gccaaattga tgtgaagaaa
     5101 tattttggtg cgatagaacc agaagaattg cttaccttac tttattgaaa ataaaaaata
     5161 gcggaatcca catatatgga ttccgctatt ttttatacag gattcgggta atttcgagag
     5221 agttgaacag tgatccgatc aatgaaagta tctttttcct gcgttagtgt tcctgtgtaa
     5281 cgatccgcta cgtctttcat catcattaag tcaaaacgtt cctgatcatc taattcttct
     5341 gtgatgcctc gactaaatag gtttgtatcg taatgtgcac tatgttcgca acaaatgacg
     5401 aaatgttcct cttgttcgtg tatcgacaaa taaatttttc gatacagtgg attttcgatg
     5461 gcatgagttt ctctaaagga atgctctaaa gcgtggatca aaagttgagc cagatcgtca
     5521 tcgttgatag gaagttcttc gggaagatct gcttggaagg ttatctgaat attccgtttg
     5581 gcagctaact tctggtaacg cgctaagata agatttgtca cctggtggtt gcaaaaagaa
     5641 cacagcattt cgaaattttt tgcttcttct aatatctttt ccacatagtt tttagctttt
     5701 gtttggtccg tatgcattaa atcttgcaga ttctccatct cttggacaaa ttccaggcga
     5761 agtgtattta cttcttggat atgctgtcga agctgctcat aatgttgctc caacaaatca
     5821 tttttgattt ttaagaaatc tacttgtctc cgatctttgt cggtatttaa gatgtgatcg
     5881 attagattgt agctgataat aacgattaga atgataatat ataaaatcgt agaccaattg
     5941 atcgttgttt gatagatacc taatgccgtt tggatgtaaa tcaagcaatg gataatggct
     6001 accaagacga tccaaggtgt acaagctaca taaaaacggt tattcttata tgcttcgcca
     6061 atgcatgcaa gtgaagtgac taaagtgctt ataacactag caatattagc aatcggcatt
     6121 aattcaggaa gttctgcagg agtaaaagtt gcataaagca gaacgccgat aaaaatcaaa
     6181 agttggaacg tacaaaaaat tccgtaccat tttttatagt aaaacattcg gctccaatag
     6241 tagcagatga tgaaagctga cgttaagata ataaataaat gagataaaaa agaatgaacg
     6301 aatggttcaa aaagaatacc acttaaaata tcttctgata cagcttcaaa tcctaatgcc
     6361 aaagcaaagc aacttaagag aataagtgag tattcaagac gtttttgttt atacatcaag
     6421 tagatcgtat agaaaaaggt ccctatagcg ataaaaacag cgattatcat cgtcaatact
     6481 tgcggcactg attgtgaata aataaaacta atcaatggtc cagttgttcc tagataaact
     6541 ttcggtggca gtccagcata gttctgataa ggagatgaga ctgtaagggt caattttttt
     6601 ccgatataat ttgctggtaa atctatcaca gccaatgaga gtccgggatt gctttcttcc
     6661 tgataatctt tccgttggta taaaatcttt tcgcctagat atacagtaat ccattgatgg
     6721 tttcctttga ttagtaaatt tgcttcgtcc aacggttttt caagtgtcgt ttccataatg
     6781 aatgtttcgt ctttatccac ggtaggaatc gtacgagtgt acttgctgtc aaacttaaaa
     6841 tcaggatcat cttttgtata gaaaacccac gaatctttta tttctttcat ttgattgctg
     6901 tccgcaggag aaaaaataaa ataaaagaaa taagcaattc ctgttaaaaa taacaatgat
     6961 aatataatga ttatgctcca ctttatacgt aaataaaaag attctttttt attatatatg
     7021 ttattaaaaa aagacataaa ggtattcctt tctaaaactt gtaatgttag ggtaaacgtt
     7081 ttttaaacta acgtcaatta tttataataa aaaatgagat aaagatgagg aaatagaatt
     7141 agaaacatat aattattaaa gggaaacatt tttacagcta taatttgaaa aaaatttatc
     7201 gttattatat gattaagaag gattgaataa agaaagaagt tgaaaacgat acctgattac
     7261 aaaaaaataa cttttataag ttctataacg ttgattatat ttttaattct tattcttctc
     7321 gtcgtcttgg ttccccgaat ttttggtttg actccttata ttattgagga caaagaaatg
     7381 agtccatatt atgcacaagg aaatctgata tatgtacgac cgaaaaatac aaatgagatt
     7441 ttagtcggag atattattac atattatgaa aattcaggaa aaaaaatctc gacgcgtcgc
     7501 gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa aagctgatac aaagacttat
     7561 attgagcctg gagtagtaaa aaagagaaat attatcggta gtcccatctt tcagattcca
     7621 tatattggac tagtcctttc aaaaacggca tttgcatgga ttcaggggtt attctttacg
     7681 attgcttttt gcctaacagg aatcacagca tggaatacct ttgtagagtt gaaaaagaag
     7741 cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat tccagagatc
     7801 caacgactct tctgaacatg aaacacgaga aaacactcat gcagcttgta gatttagtca
     7861 gttgttcgta taatgtgtga agaggaggag tagtatgcgc aatgaaatga tgcatcgtcc
     7921 tgtagtcaaa gaagaactat tggattttat gcgtacccag caaaaaaaat taccgggtga
     7981 gctaggcaaa ctggaagaag aagctcatgt agcagaagta ccagttattc cccatgaaac
     8041 agttgtattt ttgaaatttt tacttggaca gcttcagcct gaacgaatct tggagatcgg
     8101 agcagctatc ggtttttcat caagtgtcat ggccacgaca atcggagaaa acgctcatgt
     8161 tacgacaatt gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact atgaacgtct
     8221 aggactgaca gataaagtga ctttgcttga aggtcaagca gcagatatct tgccggagtt
     8281 gtctggtccg tatgacttta tttttatgga tagtgcaaaa tcaaaatata ttgaattttt
     8341 acctgaatgc ttacgtttgt taagaaaagg tggcgttttg atggtagatg acatcttcca
     8401 aggaggaacg atcttgctcc cagatgaaga gattccccgt ggcaaacgag ctatccatcg
     8461 taaattaaat gaatttcttc gtgttgtgat ggatcatccc gatttgacat cgacaatctt
     8521 gccattaggt gatggtgtga tcttaatgac aaaggaatca gaaacaatcg agttgtgata
     8581 catgattatg gcagcctgta gataatcgat tgaatcgagg gctcaattcg aacttgatgt
     8641 aaaagtttct atatttgtaa gtcaattaaa ggatacagga aatctaaata aacaggagta
     8701 gcatgaagtc aatgtagctc ctgttttcaa ttgataacct tagaacagct gaaggagaat
     8761 gtctcgtttt aaaagattga tttagtagtt gggccaaaat aattcattat ttgctaatta
     8821 aataactatt tcgtattgac agaagcaatt taattatcta taattattga tgttccgctg
     8881 attcttgtca tagtagctca gctggataga gcatccgcct tctaagcgga cggtcggggg
     8941 ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta acggtttttc
     9001 tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa aaacatcctt
     9061 atcgagtaca attataagga gcaaaaaaat aaaactcatc agaaaagagg aagaagatat
     9121 gacgatacta tctagaattt tagtgactct agttgcaatt gaattttttt atatcatgta
     9181 tatcgaaacg gtgagaactg attctgacac gacaagtagg gtttttaaaa tgagtaaaga
     9241 agagttaagc agaaaatctg ttcaaacatt gtttaagaac caaggagtgt acaatggact
     9301 attaggagtg ggactacttt atggagctta tctttcttcc gcatctaaag aaatcacaag
     9361 tatgctatta atatctattt tttttgttgc attatatggc agtttgacta gcgatcgttg
     9421 gattttactc aaacaaggtg ggctagctct gttggcattg ctttctattt tcttataaat
     9481 atagaaaaaa taagaattta tctaacagga aactttgtga aaaatattac tttatctcat
     9541 gaaatatgat attctaatga atggtatttt agaaaatttc atgaaaaagg agttagatag
     9601 gtgttcaaaa attatgtttc ctcactaaaa aaagaatttt caggttatga tctttcaaga
     9661 tttcagaaag atcttttggc tgggatcaca gtagctgctg tagcattgcc tttagccttg
     9721 gcttttgggg tatcaagcgg agctgatgcg gccgcaggtt tgattacggc tatcattgcc
     9781 ggtatcgtta ttggcggttt atcaggcggt ttttatcaaa tatcaggtcc gacaggtgcg
     9841 atggcagcta tcttgatgtc tattgcggca acaaagggaa tgcaaggtgt attgcttgct
     9901 acttttttag cgggtctatt tttacttgca gcaggacttt tccgattagg aacactgact
     9961 tcttttatcc cagcacccgt aattacgggg tttacttcag gtattgctat cattattgca
    10021 ttaggtcaga tcgataattt atttggtgtt cactcggaag gtacaaacgt tattgaaaaa
    10081 ctaagtagtt atcaaacgat aggatttccg ataaacacag cctctttgat catgggaggg
    10141 ctagtcattt tagggatgtt tatctatcca aaaaaatggg cgaaacgaat gcctagttcg
    10201 ctgctggcta tcattttggc aacagcagtt atgctattgt tccatttacc agtagcaact
    10261 gtgggtaaga ttccacaaac attgatcagt agtaatcgtt tgaatatggg agattttagc
    10321 ttttctgcct tgcaacaagt ggcagttcca gctattagca ttgctttatt aggaatgatt
    10381 gaaagcttgc tatgtggtgc ttcggctggc cgaatggcaa ataagccctt agatagcaat
    10441 caagaattaa tggcgcaagg aattggaaat ctgttgcttc ctttctttgg cgggattcct
    10501 gctactgcgg ccatcgcccg tacaagcgtg gccatcaaat ctggtgcaca aactagatta
    10561 gctggtatga tacatgcagt tgtcttactg ctctccatgc ttgtttttgc gccagtgatg
    10621 tcacagattc caatgccagc acttgcaggt gttttgattg tgacggcttg gcgtatgaat
    10681 gaatgggaaa cgatcaagga gctatttact aataaatatt ggtcagcagt tctattgttt
    10741 atccttacga tgatttgtac agttatcttt gatttaagta ttgctatcgt gatcggggtg
    10801 atcggcggct gtgtattttt tgttgtcaaa agcgctgcta tcacgatttc attagaaacg
    10861 atcgattggc aaagaatggg gctgccagaa acggaaaagc tgaataactg gacagtagtg
    10921 tatattagtg gtccactgtt ttttatgtct tcagaaaaac tcaaaaataa attggaagaa
    10981 ctaaatcatc acgatggtgt cattttctcc atgcgaggcg taccaagtat tgatttgacg
    11041 gcacgtgcag tttttgatga atttcaagaa aaagcagagc aaagaggaca aacgattatc
    11101 tatacttcct tgcagccaga agtagaaaaa caactttctc acttgtggga acatacattc
    11161 acgcatcaac atcaaacagt tgctcatgcg ttgacagctt tacatgcgaa atatactgca
    11221 attagccatt aaaacaatga aaaagaacag acaagactgt aaactgaaga ttttatggtc
    11281 ttgtctgttc tttttaatat tgctgtaaaa gtgaagctag tacttgccag tcttgatgga
    11341 taacaggtac aagggagcga ttgtagaaaa tagctgctgg atgaaaaagt ggaagaattc
    11401 gatagttttt ttctgaccat tcgtaggcat ccttttgttc tgttaattgt aagacaggcc
    11461 ctgtatagag ttgaccatga tttttggtaa ccgtatattc tttgccaagt aaacgttgaa
    11521 gaccaatgtt tccaagtgtc gcaattaatt ttggctggac cttcatcagt tcgtaatcca
    11581 atattggtgc atgcgcaaat acttcggaac gagtaggtgt ccgatttgga taaacagttt
    11641 ctgactgttg tgtccgttta ttgatccgat gagtcacgcg atacgggcgg ctccttacag
    11701 cactggtgat atacacctct tctcgtgtta agccagttga agaaagtgcc tgcatcaatt
    11761 cttttccagc ttgaccgcta aaaggaacat gcgtatcaat ctctgttttc cctggggctt
    11821 ctcccacgag catcagttta ggatgaagag gtccttgccc agcgataaat ccttctacag
    11881 gacggccgtt cattcttaaa gaagcttctt gtatcagttc ttttgggtat tccatttatg
    11941 ctcctccttt ttcttttatt ataagaataa aagaaacaca atgcgactat tttatttggt
    12001 agaactgtga ggatagaaaa gcagaagtat tgcttgaatt tttgttttac tttttgattt
    12061 taaatttagt tgactaacgg ttgctgttct ggtaagctag tactgttgta attgtggact
    12121 ccacagctac aaccgctcag aacaagtaat aagcacagca tatgctgtca cttcggatgg
    12181 cgagtctaag tatatcagaa ggaggtgctt tatagcatgt acgcaattat taaaactggt
    12241 ggtaaacaag taaaagttga agtgggacaa gcaatctatg ttgaaaaact tgacgttgaa
    12301 gcaggagaaa aagttgtttt tgacgaagtt atcttagtag gcggagaatc tacaaaagta
    12361 ggcgctccaa ctgttgcagg tgcaactgtt gaaggtactg ttgaaaaaca cggcaaacaa
    12421 aagaaagtcg ttactttcaa atacaaacct aaaaaacatt cacaccgtaa acaaggtcat
    12481 cgtcaaccat atacaaaagt ggttatcgac gcgatcaacg cttaatagcg tttgagaaag
    12541 aaggcctata tatgatcaaa ggaacattta agcgaaatga cgcaggtcag atcgtttcat
    12601 tcactttgac cgggcatgct gatgctggtc cttatggaag tgatatcgtt tgtgcgggtg
    12661 tttctgcgtt agcgatcagc actgtcaacg ggattgcctc attagccggt tttgagccga
    12721 ttgttgaaat gaatgaagaa gaaggcggtt atctttatac cgaagtcact tcaggaatga
    12781 cacaagaaca aaacaatatt gctgagattc tattagaaaa tcttttatta ggattacaat
    12841 cgatagaagc tgaaaactca gaatatatcc aaattaaaac aattaatgat aaataggagg
    12901 tgcagaccat gttgttatct atgaatctac aattatttgc ccacaaaaaa ggtggcggtt
    12961 ctacatctaa cggccgtgac tcagaatcaa aacgtctagg tgctaaacgt gctgatggac
    13021 aaacagttac aggtggatca attctttacc gtcaacgcgg tacaaaaatc tatccaggtg
    13081 taaacgtggg tatcggtgga gacgacactt tatttgcaaa agttgatggc gtagtacgtt
    13141 tcgaacgtaa aggccgtaac aaaaaacaag tgtctgttta tccagtagct caagaagctt
    13201 aataaataaa ctgctctatc tcttgtgtag caagggatgg agcttttttg taaattatgg
    13261 gatttttttt gaccatactt ttgaccatac tttcttagaa gttaatataa tttgttagtt
    13321 tttgtgttgt ttcttctttt ttgctgttag taatgtgtgt atatgtgtcc atagtagttt
    13381 ttatatttgc gtggcccatt ctttcttgga cttctttgtg atctgcgcct gcctcatata
    13441 gcattgatgc atgtgtatgt ctaaaaccat gcagaccgat ataaggtaag ccggcacgct
    13501 cacgatatat attgtatctt tctgccactg actgattaat tgtgaaatta ttttcgtcat
    13561 tggtaaacaa ataattagct ttagtaaatc ctaattgaaa taagtacttt ctttgttcga
    13621 gcttccaaaa ttttagttgc tttatagttt tttcatctaa ataaattatt cgatttgatt
    13681 ttttggtttt aggagtttca gatatataat atttatctga acgagctaca gtttttttaa
    13741 tggccatttc atttgtttta aaattaatat tgtcccaagt gagtgctaat atttcgccga
    13801 ttctacatcc gctgaatgct aatagtctaa agagcgtata gtctctgttt ttaaaatatg
    13861 gattctgttc ttgagatact tcatttagaa ataattccaa ctgttctttt gtatataatt
    13921 ttaatttctt ttctgtttta atttgtggct ttggaataag taaatttaat gttggattat
    13981 cagatgttaa accaatattt atagcatact taaaaacttt attcatatag tttatgaaaa
    14041 gagggtactg ctttgaagtt cctttttcac tccaatcatt tactaccttt tgacaatatg
    14101 ccgtattaat tttagaaagc ttaatatttc caaatttggg atatatatgt ttattaaaga
    14161 ttattttagt ccttgaatat gaactttctt ttacagtttt tttgtagctt tctagccata
    14221 attctgctgc ttctttgaaa gtagtatttg tacttgtggg cattcctgtt ttttgtaatt
    14281 ctagttctaa cctagaaagt gctgtttttg cttctttttg tgttctaaat cctcgtttag
    14341 tagtatattt tttctttcca gttagcggat caataccgag ataagtttta aagtaccatg
    14401 ctttttcgcc atttttcttt ttatattgtt ttatcattgc catcaatatt acccctattc
    14461 aaattctaat tctgccatgc tatcattgaa aaattcgatc agtccttgtt cctctataaa
    14521 ttcaagccat atttccttcc cgttaattaa tggaactcct ttttctttac gaattgtttg
    14581 atacaagtta gtcacgactt gtttccacat ttctaataca gctttatcta ttttcttatc
    14641 atcccagcgt tttttttggg gtctattttt gtttttttca atttgataat tagtagcttt
    14701 gatgataaat gtttcgtatc cggttgtgct tttttctaac cattctttga attctgtata
    14761 tatcattttt gttcaccttt ctatttttga acgtatgttc ttttttgctt aaaaaatttt
    14821 agagagctgc ataacaatag atgcgagctt ttaataattc tttcacaagt ggttcgaagc
    14881 gaacgcttaa attgttatct tctataaatt tcatgtaatt gatattgtgt ggttctaatt
    14941 cagactttgc aatatatcta tctaataatt tttcaatcat gaagcgatca gcctcatatt
    15001 ccatttttga atggagagag aatgctaagt tatataaaaa ataattatca tgatgtttag
    15061 aagcgtgtcc taattcatgt aataaggttc tttgtcttct atacttagat aaattagctt
    15121 ttattactat ggtattcata caagcaacat aatggccatc ggcatcaagg ttttcacgct
    15181 cttctacctt aacgccgagt tcattaatta tcatttcaat ttgactgtcc aaatgactca
    15241 cctacttatt tagttatctt ttttctctaa atatgcttca atcatagctg atagtatttc
    15301 cctatcatta tcggtgagcg gttttccgtc gctactcata acagatgcta aagcttcttc
    15361 cacggttagt tttctttcct ctggtgcaac tcccgcgtta ggatcatctg tgcgtcctag
    15421 caaataatct gttgatacat ggaagtagtc agcaactttt tgcaaagttt ctattttagg
    15481 actttgtgtc ttccatttat aaatagagtt ttttccaaat cctattctat tttctaattc
    15541 agaaatagaa atacctcttt ttttacagag ctctttaacc ctttcgaaaa gcatgtcaaa
    15601 tcaaccttcc tagagcgtac gataataatt tatacaaaaa gatagaaagg tagttgacaa
    15661 atctatccaa ttgtcttata tttaactcgt aagctaattt aataagctaa caagcaacaa
    15721 gaaatacacc taataaaata aacgaattcg aggtcggcaa actaagaatg ttaattttta
    15781 gggtttttct aggcttattt aactgtatat aaatataaga cagttggata gaaatgtcaa
    15841 taaaaattag ctaattttat tagcttacat atttaaagaa aaggaggaaa catcatgaaa
    15901 caaaataaaa aaactccttc cgcaaaagga gctttaataa ctttaaattt taaagaccaa
    15961 aagtttagtg ctgttgttga tgaaattcat ccacaagagc atcaaaaaaa ttcttttgca
    16021 actgacgaga aaaagactca gaaacgctct gagaaagttc ttcgaataat tcaatttctg
    16081 ttggaaaaga ttcgtcattt tggaagtcta ttttgaattc tttattaaca gtcttgaata
    16141 cggtttgttt gaataaggcg tcgacatcca tattaaattc ttccatacta ttttcacctc
    16201 gctttctatt caaaattata tcaaagaaag gaatgagcaa aaatgaacac accgcaaatt
    16261 tttaactttg aacaacatga agtaagaaca gtaacaatac atgatgaacc attttttgtt
    16321 ggaaaagatg tggctaaagt tctagggtac caaaatggta gtagagatat taacagacac
    16381 gtcgatgtag aagatcggca aaactaccaa aacggtactt ttgaatcgcc tagagggtta
    16441 actattatta acgaatcagg tttgtactcc ttaattttag gaagcaaaca accaaatgct
    16501 aagaaattta aacgttgggt gactagcgaa gtgttgccag caattagaaa acatggaggt
    16561 tatctaactc cagaaaaagt agaagaagct ttgcttaatc cagatacaat catccaatta
    16621 gcaactaagt tgaaagaaga acgtactgga agactaatcg cagaacaaaa aattgcagaa
    16681 tacgaaccca aaatttcgta tttggatagt atcttatctt ctacagattc agtaactatt
    16741 agtcaaatcg cggctgatta tgggatgtct ccacaacaga tgaacaaact acttcataag
    16801 ctaggtattc agaaaaaagt aggcaaccaa tggttattat gcaaaaaaca catgagacag
    16861 ggttacacaa aatctcatac aactgagatt ccgaaatctg atggcggaac aaaagttgtg
    16921 atgaatacaa aatggactca aaaagggcgt ctgttcattt atgagtcgct gaaaaaagaa
    16981 ggatatattc cagaaatcga tttattagag gaggaatgac gatgtcacaa gaatttattt
    17041 tgaaagtcag aattcaatta gcaaaatacg gaaagtctca gaactggtta gctgatacta
    17101 tcggaatttc tagaccatat atgtcggaca ttatgaatgg gcgtagaaaa ccagataaac
    17161 aaatcaagcc aattgaggca gcactagcag agttagagaa ggagaaataa catgcaaata
    17221 acaattccag ataatttagt agtttccgaa ttaactacac agattacgaa tgctgttctt
    17281 aattcattgg acgaacgatt acaccttatg aacaaatcag tagagcttcc tccatatcca
    17341 aacaaatcag aggtaaaaaa agttttaggc attggtgatg acaaattaac acattggata
    17401 agcctaggct taaaaacaca gcagtggagc aagttagaca tcagaattga acgatcggaa
    17461 ctccaaagat ttttgaaaga aaactttgag ttctaaaggc aaaggagaat gattttatgt
    17521 cctacacatt gcaacaagaa catcaaattc taggtctgat taaacagcgt agaaaacaat
    17581 tacaagatga tcgtgcagcg cttagaaaat ccgatgagct atcagataga caagctgaac
    17641 taattgcttc tgaacttgag gatttgagaa tgctagaaat aaaaaatagg gagattagat
    17701 tatgaagaag acagacacac tttttatagg attcattttg gggttattag tgattgtagc
    17761 gcaccaaagt attatcggag gaagcttgtt cgcagcattg atggttttaa tcaatctgct
    17821 tgactcaaaa gaaaggagca actatggcac gagaagaagc gctaaaaatc ggtaaagtga
    17881 ttgctgataa ttggtgggca aatagccgtt ctattatttt aagcaagcaa catatcgaaa
    17941 agaaaaaagc atggcaacaa ataaaaaagt gactccgccg accaaagcaa tgagtcactc
    18001 aaaaaatcat aactaaggag attttaacat atggaaaatg aactttccac tctagatcaa
    18061 tatttggccg atcctgattg gggcaaatcg aatttacaac tggttgctaa caagtacgaa
    18121 aaatcaattg atgaagcaat tgttgcattg actgatgcag cttccataac cgctgatgac
    18181 agtaaatgga ccaagagaga cttgggcatt ctaaaacgag gcgttaattg gcttgaagat
    18241 cagtacagag aagaaacaaa agagaagtga tatgagtgtt taaaccacta atagattcat
    18301 actcggcggt actaaagaaa tttaaaggta acgacattgg tgcaacaatc aacgaagaag
    18361 taaatatcga acggctgaaa acgatgtatg acggatatga tggcgatcga gtcattgaaa
    18421 ttcgctttat tgatccacga cggttcacag tacagcaaag aaacttcatc tatgcgctca
    18481 taggcgatat tttcatcgat acaggcatgc caacggactt ctggaaggaa ttcttctact
    18541 tccgttttga aggtgtcaca gggcgcgaaa taagcctcaa agacgaatcg aatacaaccg
    18601 tgagtgatgc caatatctta gcgaatatca tcctagattt catctttgaa catcatattc
    18661 ctttcaaaga aggatatgag attttaccag cgaatcaaga gtattacttc tacaaatgca
    18721 ttacaaaaag agtctgctgc atctgtggca aaacaggagc tgatatcgat cactttgaca
    18781 aagcgctggg aagacgaaag cgcaaagaag ttgatcattc agagtacaca tttgcagcac
    18841 tctgcagaat ccatcacaca gagaaacaca aaataggtgt gatcaatttc aaaaataaat
    18901 atcaaatcaa agggatcaag ttaaaccagg aaacaattaa aaagttaagg ataggaggat
    18961 aaatttggct gagataagtt ggatcaaact taaaactact atgtttgacg atgaaaaaat
    19021 acgattaatc caagctgttc ctgagtcgga tgccatcatc gttatatgga ttcgattact
    19081 agttttagca ggaaagacta acgacgatgg tctgatatat atccagagga acatgcctta
    19141 taccgaagaa atgcttgcta cattgtttgg caaaaacgta aatacggttc gcttagcgct
    19201 aactacattg gcaaatttca acatgattga tctaagcagt gatggactaa ttgccatcag
    19261 taattgggaa aaacatcaaa atatcgaggg tatggataaa gtaaggctaa aaaatgctga
    19321 aagaaaccgt aaatacaggg aaagaaagag acaggaacgt ctcaaattgg aaaatgacgt
    19381 tagcgtgacg tcacgtgacg gtacagataa agatatagaa gaagataaag atatagataa
    19441 agaagaaaag aaaggtaagt attctgacga acacttacgc cttgctaaaa agttgcaaag
    19501 taatttaact gaagattttc caaaagaaat gaacaaagta gatatcgaaa aatgggcaga
    19561 cacaatcagg ttgatggaag aaagagataa agcatctata gaagcgattg agtatgtgat
    19621 caattggcta cctacaaatg aattttggtt tggaaatatt agaagtgcta agaaattgag
    19681 agaaaaattt gagaagctca aattcgaaat caaagcagac aagaagaatc ataaaaagca
    19741 aagtcaaaaa ctacagtaca gcaatcctag tgaatatgac gacttgccaa tttaaaaagg
    19801 agatgcatca catggaaagc ctagcaaatg ctatggagaa actaataaga agagtattag
    19861 tgcaaagcgg aaaatgtcca gaatgtagcg aacctttgta tagttggcga gctaaaaata
    19921 aggatggttc agaacgttgt aaaccaacat gcatgagttg tggttataaa gcgttacgtg
    19981 tgaaagagga tatacagacc gaacggatat ataacgatag cttaaaagca cgagcgttga
    20041 gtttttttca aaatggttcg gtattaacag ataaaacttt gtttaaatgc aaaatggaaa
    20101 attatcacgt agtggatcaa gaaacaaaaa tcgctttaga aaaagcaaaa agctatacga
    20161 atgaggttct gctgaaccat cctgcacatt tcattctatc agggaaatca ggaagcggaa
    20221 aaagccactt atcaatggcc acagcttggg aaatacttga gcgctcaaat tatgacaaga
    20281 aaatactttt tataagctat caagaattat tagagcaaat aaagttttct tataacaata
    20341 ctgaactgag aaaagaaatt gaaggatcgc ttatagccga tattaaaaca actgatttgg
    20401 tggtttttga cgatattgga gctgaattag gtagtggggt atcaaatagt aggcagttta
    20461 caaacaacac gttaaacacg ctcttagaag ccagacagaa caaggcaacg atcatcacaa
    20521 caaacttatc tggtcctgaa ctaagagaag cctacggtga aagaattgtt tctaggatat
    20581 ttaagaattc agaaggttat gcgctgaaat tccaacaaac agcagacaag cgcataaaac
    20641 cagtgaaagg tagtatcgca tgaataaata ccgtaataaa aaaactgttc atcgaggtat
    20701 caagtttgat tctatcgcag aagcagagta ttacgatcta gccttgtggc aagctgaagc
    20761 aaatggctgg aaagtaaaac ttcaggaaag atttgagctg atgccgaaat ttgaactaga
    20821 cggaaa
//
LOCUS       DAYLZO010000044        20617 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.045, whole genome shotgun sequence.
ACCESSION   DAYLZO010000044 DAYLZO010000000
VERSION     DAYLZO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.045"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(9..482)
                     /locus_tag="ACGVJG_001475"
     CDS             complement(9..482)
                     /locus_tag="ACGVJG_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFJ6557424.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(587..1450)
                     /locus_tag="ACGVJG_001476"
     CDS             complement(587..1450)
                     /locus_tag="ACGVJG_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFJ6557425.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1646..3745)
                     /locus_tag="ACGVJG_001477"
     CDS             complement(1646..3745)
                     /locus_tag="ACGVJG_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFJ6557426.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3965..5074)
                     /gene="rpoD"
                     /locus_tag="ACGVJG_001478"
     CDS             complement(3965..5074)
                     /gene="rpoD"
                     /locus_tag="ACGVJG_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFJ6557427.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5098..6939)
                     /gene="dnaG"
                     /locus_tag="ACGVJG_001479"
     CDS             complement(5098..6939)
                     /gene="dnaG"
                     /locus_tag="ACGVJG_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFJ6557428.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7121..9436)
                     /locus_tag="ACGVJG_001480"
     CDS             complement(7121..9436)
                     /locus_tag="ACGVJG_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFJ6557429.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9621..10481)
                     /locus_tag="ACGVJG_001481"
     CDS             complement(9621..10481)
                     /locus_tag="ACGVJG_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFJ6557430.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10540..11172)
                     /locus_tag="ACGVJG_001482"
                     /pseudo
     CDS             complement(10540..11172)
                     /locus_tag="ACGVJG_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            11339..12100
                     /locus_tag="ACGVJG_001483"
     CDS             11339..12100
                     /locus_tag="ACGVJG_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFJ6557431.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12126..14717
                     /gene="mprF"
                     /locus_tag="ACGVJG_001484"
     CDS             12126..14717
                     /gene="mprF"
                     /locus_tag="ACGVJG_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFJ6557432.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14905..16092)
                     /gene="tuf"
                     /locus_tag="ACGVJG_001485"
     CDS             complement(14905..16092)
                     /gene="tuf"
                     /locus_tag="ACGVJG_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFJ6557433.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16460..16816
                     /locus_tag="ACGVJG_001486"
     CDS             16460..16816
                     /locus_tag="ACGVJG_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFJ6557434.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17057..18061)
                     /locus_tag="ACGVJG_001487"
     CDS             complement(17057..18061)
                     /locus_tag="ACGVJG_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFJ6557435.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18227..19078
                     /locus_tag="ACGVJG_001488"
     CDS             18227..19078
                     /locus_tag="ACGVJG_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFJ6557436.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19095..19340
                     /locus_tag="ACGVJG_001489"
     CDS             19095..19340
                     /locus_tag="ACGVJG_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557437.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            19527..20231
                     /locus_tag="ACGVJG_001490"
     CDS             19527..20231
                     /locus_tag="ACGVJG_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557438.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         5841 a         4223 c         3603 g         6950 t
ORIGIN      
        1 ctagacactc agacggactg gtttcttttt tctttttcat agcattcctg acagattcca
       61 tggaacgtta gccgatgatc ttttactaaa aaatgataac gttcttctac tacttgctcc
      121 acttcaagta aaagatcctc ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa
      181 tgatgatgga aatgtttggc gccttctttt ctcaaatcat aacgggctaa tccatcatta
      241 aaacttactt tatcgacaat tttcaattct gtcaagattt caagggttcg ataaacagta
      301 gcaagtccaa tttccggact cttttttttc actaaaaaga agatttcttc tgcagataaa
      361 tgctctttct cattttccaa aagcacaagc agagtagctt ctctttgcgg tgtcagctta
      421 aaccccgctt catgtagctg ctgttttgtt tttttcatcg ccgaaatagc attcatgacc
      481 atttactcaa ccccaacaat ttataataat tacaaataaa aaaagatata acactatgtt
      541 aattaaaatt attataagct agttaagaaa aacatgcaag ggattctcaa ttagattttt
      601 tgactagttt tgtcacaccg ttttcttgtg taatcaatcc ttgtttcaaa agattaccta
      661 atgcgcgctt aaatgctcct ttgctgatac caaaagcttt catgatttct tctggatccg
      721 atttatctgt aaagttgatt tgctgatctg cagaacgttc taaaatggtt aaaatcattt
      781 gtgcatcatc tgatatagct tcatatgctc ttggcttcaa tgaaagattc agtacaccgt
      841 ctggtcggac accaatcact cgacctttta tctgctcgcc tagacgaggt tcattatatc
      901 gttcagaagg atggataaac ccaagataga aatcttccgt taaaacgtac gtacctgtta
      961 atttcaagcg gtatgctgtt cctatcacat ctttgttttt catttcttct gttccatatt
     1021 tgcttaaaga tttgaatatt ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat
     1081 caactctaag ggaaatcatc aagcgatccc cttttttagg ccatagctct gtcattgtcg
     1141 gcaattcatc aagagatacg acaaagtctt tatctggaag accgatatcg acaaacgcac
     1201 ctaaatcttt tcgtgtagca gtaacgattc caaaagcgta atgaccgata cggcttttag
     1261 gaatttgtgt agtgatccga ttttcatgtt tttgattttg gtaaccgaat ccttcaactg
     1321 cttcaccgat tttgtgttca ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt
     1381 ggacaaagta gtttttttca ttttcatcaa taataatacc tgtaaatata gttgctaata
     1441 aatcgttcat ttttttctcc ctctatagtg aaaggttttc ttaatacatt tctttgctat
     1501 cataacataa tgttatctac ttgaatagct gagccttggt ttggaaaggt gaaaaaagga
     1561 gtatgacaac agttgtgtca tactcccctt tttcgaataa acggtgttaa acggcaggct
     1621 cacagcctct ttcatttgcc taaaattaat tgctcccatt tcctccgaac catccagttt
     1681 tacagttgat ggttacgatg taaaatggca aagcactttc tccaatgcct ttcatcaatg
     1741 cagattttgt tgaagtggca tatagattat aatagccttc cccattttcg attctggctt
     1801 tttctaatct gtaacctcct tcaacgatat atccacgttg gatacatgta gcaatcactt
     1861 tgctttggac acctggtgcc cacgtccatg ctggattaga tgcatcggca atatcacttg
     1921 ctctggcaga tttgattggc tgattcgctg agtttgaaga ggaaggggtc gaggatgccg
     1981 cttcttgctg tgctgcttgt gcgaccgcag cttgttgagc ggcttgagct gcagctgctg
     2041 cttgagcggc ttgttttgct tgttcttcag ctgctttttt cgcttcttct gcttttttct
     2101 tttcctcttc ttcttttttc tctttatcag tgagcatctt atctacttgt ttcaattgtt
     2161 cattcaactg agcagataga tctgtatttt tcactttttc tactgcttct tttgccgtct
     2221 tgtattggtc gcgtgttgct gaatccacta cttttccgtc tttaaagact atagctgttt
     2281 tttctttagc tgtttgcaat tcagcatatt gatttttagc ttctgtttgt gcttgttgaa
     2341 tcaattttcc aaaatctgtg ttatccgttt cgattgcttg gatctcttta tctgctttca
     2401 acttgatgtc atctgctaga tgatcatcga cgatggcagg tgctatgaac cactcattca
     2461 ctttttggat atctcggatc ttagattgga tatcttggta agttgtttct aactctgaat
     2521 atcctttttc attcttaaga ctggataaat cattttttag ttttgataaa tcttgatgaa
     2581 tcatatctgc acgaataaat tgatggtgat catctgtata aaaggcagca agtttctcat
     2641 gaagttcttc catttgttgc tgtctttttg cttctgctgc tacttcggct tctacttttt
     2701 gatgatggtc ataataagcc cagcttcctc caccgagtag aagtactgct gctaaaacga
     2761 gataaggtac tttttttgat tttttctttt gcgctttatc tggatctgtt ttttctaagt
     2821 ttgattcttg atgtgttttt tcagtatctg gcacgattgt tattgatttc gcttctgctt
     2881 caaacagctt aggctggttc tcttcttttt ctatttttgc ttcaagatca gtaccctctt
     2941 tgcttacaga ttgattttca gcatcggatt cactatttat ttcatcgggc tctgttgact
     3001 ttttgtctgt gttttcgagt atttcttttt tctctgcttc cggcagttta ttcgtttttt
     3061 ccttttctaa ttgtttttca gtaaatactt cagttttttc tgtgatatcc gtttttacgt
     3121 cttttatttt tactgtttct ttacctgtgg cgttttctct atcagagtct ttttcagttt
     3181 cttgttcctt ttctggctct actttgtttt cctgtgcaat tttttcactg atcattttga
     3241 gcagctcttt cgcgtgctct tcttttgtat cttcacgatg tgctttgata taagcggata
     3301 gtatcgaatt ttcttcctca ttatcgacga tttctttcag ttcagctact cttctttttt
     3361 gttttgattc aagcgtttct tcttgattct ttcctgattc ttccgaagtc tcatgaacag
     3421 ttgctttctt ctgttcattt ttctctatat tagttacttg atcgtgagca ggtggttctt
     3481 cgtcttctgg ttctgctttt tcgaataatt ccattacaga ttctagaggt aaatctttca
     3541 attccgacca ttcgattgga tcgtttaatt ctgtatcagg aaagactgca tcttcagttg
     3601 atttatcctg catagctgca tctgtatttg tttcttcaat tgattctttt atttgctgat
     3661 cttctgcagt tatttgatga ccgcagtctg gacagatagt attctgctcc atttttttcg
     3721 atccgcaatt tggacatttt ttcatcactt agcgatcggc tcctttacta ggtttacgct
     3781 accatttaat ctatcatatt ctatacgtca cgccaatagc ttatttttag gatttactta
     3841 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
     3901 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
     3961 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
     4021 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
     4081 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
     4141 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
     4201 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
     4261 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
     4321 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
     4381 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
     4441 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
     4501 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
     4561 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
     4621 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
     4681 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
     4741 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
     4801 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     4861 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     4921 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     4981 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     5041 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     5101 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     5161 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     5221 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     5281 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     5341 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     5401 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     5461 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     5521 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     5581 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     5641 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     5701 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     5761 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     5821 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     5881 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     5941 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     6001 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     6061 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     6121 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     6181 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     6241 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     6301 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     6361 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     6421 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     6481 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     6541 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     6601 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     6661 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     6721 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     6781 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     6841 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     6901 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     6961 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     7021 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     7081 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     7141 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     7201 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     7261 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     7321 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     7381 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     7441 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     7501 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     7561 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     7621 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     7681 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     7741 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     7801 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     7861 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     7921 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     7981 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     8041 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     8101 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     8161 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     8221 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     8281 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     8341 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     8401 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     8461 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     8521 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     8581 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     8641 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     8701 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     8761 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     8821 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     8881 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     8941 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     9001 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     9061 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     9121 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     9181 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     9241 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     9301 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     9361 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     9421 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     9481 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     9541 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     9601 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     9661 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     9721 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     9781 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     9841 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     9901 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     9961 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
    10021 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
    10081 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
    10141 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
    10201 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
    10261 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
    10321 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
    10381 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
    10441 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgt actccttctt
    10501 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
    10561 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
    10621 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
    10681 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
    10741 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
    10801 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
    10861 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
    10921 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
    10981 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
    11041 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
    11101 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
    11161 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
    11221 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
    11281 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
    11341 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
    11401 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
    11461 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
    11521 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
    11581 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
    11641 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
    11701 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
    11761 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
    11821 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
    11881 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
    11941 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
    12001 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
    12061 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
    12121 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
    12181 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
    12241 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
    12301 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
    12361 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
    12421 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
    12481 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
    12541 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
    12601 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
    12661 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
    12721 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
    12781 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
    12841 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
    12901 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
    12961 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
    13021 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
    13081 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
    13141 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
    13201 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
    13261 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
    13321 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
    13381 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
    13441 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
    13501 tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat tttatttcct
    13561 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
    13621 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
    13681 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
    13741 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
    13801 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
    13861 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    13921 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    13981 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    14041 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    14101 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    14161 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    14221 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    14281 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    14341 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    14401 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    14461 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    14521 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    14581 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    14641 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    14701 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    14761 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    14821 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    14881 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    14941 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    15001 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    15061 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    15121 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    15181 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    15241 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    15301 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    15361 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    15421 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    15481 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    15541 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    15601 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    15661 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    15721 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    15781 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    15841 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    15901 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    15961 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    16021 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    16081 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    16141 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    16201 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    16261 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    16321 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    16381 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    16441 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    16501 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    16561 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    16621 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    16681 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    16741 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    16801 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaaagag gctgggacag
    16861 aagcgtttaa ctccaagaaa taagaaggag ttgcttctgc ttccgccgtt tatacgtgtt
    16921 tagagcgtga gacaaaagtg ttctttactt ttgtctcacg ctcttttttt gttatattcg
    16981 tcttttccga tccttctctt gacacaattc atttaactga tcactggtac ttgaacacct
    17041 aatattctag tcatttttat aaacgattca atacttcatc cacatttttg atcatcacgg
    17101 atatcgtacc atccggatta ttcacaaatt caatcaaatc tgggttgcga tatacatcta
    17161 ctggtacgat cagttcgatc ccgtttgata gcttgaattt ttgttttcca aattttttct
    17221 cagaaatttc ttgtacttct tttacaggag gtgcttctgg gacaaatcct tgctcttgta
    17281 cttcttcttt aaaagccatt tttgccgtga tgttttcctt gaagactttt tccgcaatca
    17341 tctcatgatc caaccgacct ttttcttcta tcgtatcata gacagcttct ttcaaatcag
    17401 ccatgatatc aaattcttct gtctcaaatt tttttccaag atgcttggct actttttgga
    17461 tcacttttac attttcttct aaagaaggtg caggaacact ttcgatcact cttccagaaa
    17521 aataaagctc ttttttgcca gaaaactcat attttttttc aattaattcg taggtccaat
    17581 cagtcaaatt cacagcgaat gcttcatctg ctttttgtgt tttcgcccct agaattgctc
    17641 gattcaaaat caagcggttg tcgatccctg cttcgtctgc ttcaacaaag tgagtgtaag
    17701 cctcacgata attgactttc aataaagata agtacatcac tgtatctaat tcatacaaga
    17761 caacaaatac atctgcacta ggtgcttctt cactttcttg gtagatatcg aaccaccgct
    17821 gaacgattgc ttcacttgct tccacaaaat tttcttgagc gattttaagt gattcaacga
    17881 tcatgctttc ttctgctagt tgtccagttt ttgtttgagc agaagagatt ttttggatct
    17941 tcttttgaag aaaatcacga atatattctt ttgtcagatc cagctctttt tgtgaaaaga
    18001 ctggagaacc agtttctctg tcaataatat gcagaattgc tttttttaaa tagatatcca
    18061 ttttctcatt cccttctgtc ttagtttacg gaaaaaaaat caaaaatacc agaatcaaaa
    18121 tcattttctt ttaaacaaaa atttcaagag tagaaacaaa atggattctt tttttcgaaa
    18181 aatgcgctac acttatagtt gaacgaataa aaaaggagct gaaagtatga attcattaac
    18241 aagcacttat cgtctatcaa atggttacga aatcccggta gtaggattcg gcacatggca
    18301 aacacctgat ggggatgtcg ctgtttcctc tgttaaagaa gcgctggctg caggttatcg
    18361 ccatatcgat actgcccaag gatacaagaa tgaagaaagc gtcggccaag cgattaagga
    18421 aagtggaatt cctagagaag agatattctt gacaacaaaa ttatggaatg caaaccacag
    18481 ctatgaactt gtgatgtctt cttttgaaga atcattgaag aagctccaaa cagattattt
    18541 agatctgttt ttgatccact ggccaaaccc tgttgctttt cgtgataatt gggaacaagc
    18601 aaatgcagat acatggcgag catttgaaga actgtatgaa gcaggtaaaa tcaaagcaat
    18661 cggtgtgagc aatttcttac cccaccatct agatacacta gcaaaaacag caaaaattat
    18721 gccgatggtc aatcaagttt tccttgcacc aggcgaatta cagacaactg tagttgagta
    18781 tgcaaaaaaa catgatatga tcctcgaagc atatagtcca ttaggcactg gtaaaatctt
    18841 cgatgtgcct gaaatgaaac aaatcgctga agcacatgac aagacgattg cacaagttgc
    18901 tttgcgttgg tcgctgcaac acgaattcct tcctcttcct aagtcagtga caccaagcag
    18961 aatcaaagaa aatactgaat tatttgattt tgaattaaca gaagaagaaa tgaaacaaat
    19021 cgatcagtta gatggtgtag tcggcaaagc aaaagaccca gatactaccc aattttaagt
    19081 cgaaaggtga ttttatggat ccaatcgaac gcttgaattc tctctcggaa gaagttaccc
    19141 agacttttca ttccgatttt gtctttttga tcgatgcaga gaaaatccaa cattttcctg
    19201 caagaaactg gacacatgat caaatcatcg aagaactgaa aaaacggttc gatcattcgc
    19261 taatggtcaa accctggcac gaacatgaag tcatttattc acctgaacta cctgtttttg
    19321 ccttgatacc gaaaaaatag aaagaatgga acggattatg tatatataca taatccgttc
    19381 cattttttat ttttctttca aaaaacggga aaatacgcaa atttttctgt tgtaatttgc
    19441 gtatacgcaa gtttttttgc attactcctt ttcatttggc tgtttttttc ttataatcga
    19501 aagggtatga gcgaaggaga gaacatatga agaaaaaaca gaaacaaaaa ttattaaatc
    19561 aattccgtcc ttcattggat ggtgtaagag gacaattatt ccgttctcta gaagaagaga
    19621 gcttgcgacg ttatggtttg ccgttacaag tcatgcttga tcccaaaaac cgtcaaatct
    19681 taacgatcgg attagctggg aaaacaaatg aagatacacg gatcaacgtg ccaatcgatg
    19741 acaacttcaa tactgtatta aagagaattc gttcaggaga aaacgggatt ttcgaacgat
    19801 tccgtgacaa tcttcttatt gaaatcgtgt cttactggaa tgatcaacga ttgaaaaacg
    19861 atgaaccaac tgcgcaagct gttgcgacac cagttacaga agaaacagtc agtgtgccag
    19921 aagaaaaaac ggaaaaacca aaagcggaca caactgaaaa tacacgctca actgatacga
    19981 atgccgcttt aagttttgct gatttttcaa aagccgttgc tgagtatcct aaattttaca
    20041 cagaacaaaa tgaacatgaa gtagttatta atgaaaaaaa tgctgatgaa cctcgtaaat
    20101 tagcaactat ttctatgact actgaaaatg agttcgttat cgaaaaagca ctcgaacgga
    20161 aatacaaagt caaattgact ttgattcctt tgatcgaaca atttgcagcg acagcgatta
    20221 ctgatcgata aaaattctat ttgtcataaa aggctgggat ttaaaatccc agccttttta
    20281 gtcatataat aatttctaca gcaagtaaaa agaaattcct tctaacatca ggctcaaata
    20341 tcagcttgac ctgtccaaca aatcacctat gatcctacaa tgttaggcga cttctcttct
    20401 acctgaagat atcttggagt tgcctgattt cttaagatgc ctaaccttaa ctataaaaaa
    20461 ttctaataat aaacatattt tctgatagag aaaaaagcgg ttctataata ttttgtgttg
    20521 gtggaaaatc gaaagcgatt ttctactaac acttttttct tcgtttcaac tcaaaaagag
    20581 actcactttt ggtaaaattt agtcgaccaa aacgtca
//
LOCUS       DAYLZO010000045        20549 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.046, whole genome shotgun sequence.
ACCESSION   DAYLZO010000045 DAYLZO010000000
VERSION     DAYLZO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.046"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..332
                     /locus_tag="ACGVJG_001491"
     CDS             <1..332
                     /locus_tag="ACGVJG_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFJ6557439.1"
                     /translation="AYSLCTAAILICYLLVGIYQVQFSLKEGIKKQVVIGGIAVVFEL
                     MGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKVMIGLICVAALLSIGLL
                     AVGWIKI"
     gene            346..1464
                     /locus_tag="ACGVJG_001492"
     CDS             346..1464
                     /locus_tag="ACGVJG_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFJ6557440.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(1524..2816)
                     /locus_tag="ACGVJG_001493"
     CDS             complement(1524..2816)
                     /locus_tag="ACGVJG_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFJ6557441.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            2966..3526
                     /locus_tag="ACGVJG_001494"
     CDS             2966..3526
                     /locus_tag="ACGVJG_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6557442.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3655..4032
                     /locus_tag="ACGVJG_001495"
     CDS             3655..4032
                     /locus_tag="ACGVJG_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFJ6557443.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            3965..6037
                     /locus_tag="ACGVJG_001496"
     CDS             3965..6037
                     /locus_tag="ACGVJG_001496"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFJ6557444.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(6248..7195)
                     /locus_tag="ACGVJG_001497"
     CDS             complement(6248..7195)
                     /locus_tag="ACGVJG_001497"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFJ6557445.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            7441..7905
                     /locus_tag="ACGVJG_001498"
     CDS             7441..7905
                     /locus_tag="ACGVJG_001498"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFJ6557446.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(7967..9010)
                     /locus_tag="ACGVJG_001499"
     CDS             complement(7967..9010)
                     /locus_tag="ACGVJG_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFJ6557447.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(9387..10352)
                     /locus_tag="ACGVJG_001500"
     CDS             complement(9387..10352)
                     /locus_tag="ACGVJG_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFJ6557448.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            10586..11431
                     /locus_tag="ACGVJG_001501"
     CDS             10586..11431
                     /locus_tag="ACGVJG_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFJ6557449.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            11622..12809
                     /locus_tag="ACGVJG_001502"
     CDS             11622..12809
                     /locus_tag="ACGVJG_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFJ6557450.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            12815..13171
                     /locus_tag="ACGVJG_001503"
     CDS             12815..13171
                     /locus_tag="ACGVJG_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFJ6557451.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            13173..13511
                     /locus_tag="ACGVJG_001504"
     CDS             13173..13511
                     /locus_tag="ACGVJG_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFJ6557452.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    13587..13843
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13906..14940
                     /locus_tag="ACGVJG_001505"
     CDS             13906..14940
                     /locus_tag="ACGVJG_001505"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557453.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            14933..15619
                     /locus_tag="ACGVJG_001506"
     CDS             14933..15619
                     /locus_tag="ACGVJG_001506"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFJ6557454.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            15643..16491
                     /locus_tag="ACGVJG_001507"
     CDS             15643..16491
                     /locus_tag="ACGVJG_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFJ6557455.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            16688..17461
                     /gene="sufC"
                     /locus_tag="ACGVJG_001508"
     CDS             16688..17461
                     /gene="sufC"
                     /locus_tag="ACGVJG_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFJ6557456.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            17474..18760
                     /gene="sufD"
                     /locus_tag="ACGVJG_001509"
     CDS             17474..18760
                     /gene="sufD"
                     /locus_tag="ACGVJG_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFJ6557457.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            18757..19992
                     /locus_tag="ACGVJG_001510"
     CDS             18757..19992
                     /locus_tag="ACGVJG_001510"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFJ6557458.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            19979..20449
                     /gene="sufU"
                     /locus_tag="ACGVJG_001511"
     CDS             19979..20449
                     /gene="sufU"
                     /locus_tag="ACGVJG_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFJ6557459.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
BASE COUNT         6879 a         3839 c         4394 g         5437 t
ORIGIN      
        1 ttgcctattc tttatgtaca gcggcaattt tgatttgtta tctacttgtc gggatctatc
       61 aagtccagtt ttctttaaaa gaaggaatca aaaaacaagt agtgatcggt gggattgctg
      121 tagtttttga attgatgggc atcattttag caggattttc ttatgtcctt ttatgtagta
      181 ttgcctatct tcctggtttt tgcttctact ggatcgcttg tcgtgaaacc aaacatcaaa
      241 ttacgggaaa agaaaaagta atgattgggc tgatttgtgt ggccgcactg ctttcaatcg
      301 ggttgcttgc tgtaggctgg atcaagatat aagggggaaa gaaacatgaa aatcgcaaat
      361 ttcggtgtag aagaatggct aaataaatgg gaaaagaaag cggtatatga tattgcacaa
      421 agctcgatcg aagcattgac attagacgaa ctcgttggct tagatgggac tagcgtaagt
      481 aaatttttcg aaaaacgcaa aaacgaacca ttggactatg gctggatcga aggatcagat
      541 gttttcaaac aagaagtcgc ttctctttat caaaccgtcg atccggaaaa catcttacaa
      601 acgaatggag cgactggtgc taatctttta gctctatacg cgttagtgga agcaggagac
      661 catgttgttt cgatgcttcc tacttatcag caattatatg acatccccaa atctttaggt
      721 gccgctgttg actttgttca tttgaaggaa gaagaagatt ggcagtttga tttagaacag
      781 ctagaagcct tagtcaaacc tgaaacaaaa atcatctgtt tgaatagtgc aaacaatcct
      841 acaggcacat tgctagatcg gaaaacttta gaaaaaatcg cagaaattgc tcgtacagta
      901 gatgcatatg tattgataga tgaagtatat gcgcctttaa cggataaagg tgaattcttg
      961 tcgatcgtag atgtctatga aaaaggtata gcaaccaatt ccctatccaa aacctactcc
     1021 attccaggta ttcgtattgg ctggacagct actggacctg agttggcaga agtttttcga
     1081 aagtatcgag actacacgat gatttgcggc ggagtcctgt cagatgatct tgctgtacat
     1141 gcattgaaga ataaagaaaa aatattggcg cgaaacaaaa agatcattac agagaacctt
     1201 gtgattttaa aacaatgggt agccaatgaa ccgaaagttg aacttgtcgc tcccaattac
     1261 gtctctactt ctttcattaa attagccatc gaagaagatg accagacatt ttgtatcaat
     1321 ttattagaag aaacaggagt actattagta ccaggaagtg cctttgattt accgaagcat
     1381 gcccgactag gttactgttg taaaaaagaa accttggaaa agggattatt cctcttatct
     1441 gagttcttga aaaaacaggc ctaaacaaaa acactgcaag gacagtctgt gacaactgtt
     1501 caagcagtgt ttttcattta cgcttattgt ctatgagaag gagagtgccc tgaagctatg
     1561 gagactcctg aagctttttc ttctgcagtt tcttgctgat ggatctcttg tgtattgtgt
     1621 cgtacaagca agaagttgac caaaacaaga agagaagtcg aaagaaagac gccgcgataa
     1681 ccaaatgcac tggaaacact ggagccgatc ataggcccta ctacgttacc agttgcttgg
     1741 aaagattggt tataactgaa gatccgtcca gcagcttgat gaggcgaata ttttgtgatc
     1801 aatgcttgga cagcagggag cagacaagca tcagagatac caatcaaaaa acgtaaggca
     1861 gctaattgcc aaacattcgt gacgaatgcc attgggatat aaaccagaat cgcaaaaccc
     1921 aaaccaatcg caagtattct ttcactccca atcttgtcac ctaagcgacc aaatcttgga
     1981 gcggctatca aagtggcaat cccaggtatc gaagcaatga ttccgctgat caacgtaaca
     2041 ttcccatgtc catgcagcaa ttggcgaatg tacagactga tgattggact aatggaattg
     2101 ttggatgctt ggatgatcat cgttgtgacg aacataccga tgacgacgtg tggatatttc
     2161 aagtctttga aaatctgttt ggcagaaacc atgttttctt ttttcactgg gacaaatttt
     2221 tcatggacga aaaacagact caacaagaag acgaaaaata agatgatccc tgtaatgaaa
     2281 aaggtagggc gatagccaaa cgctgaagca gagatcccgc caagaagcgg tccaagtaat
     2341 gttcctgtaa cagaaccagt tgctaaggtt ccaagaactt gaccactttt ttcttttggc
     2401 gtcccagttg cgacaagggc agtggcatta ctgatataac cagaaaaaat cccttgaagc
     2461 agacgcaatg cgactaattg atacacacta gtcacgcaac ccatcaaaga aatgacgatt
     2521 gccattccta atgaagcacg caacagcatc aattttcttc ctttttggtc ggctaatttt
     2581 ccccaccaag gagagataag cgtagtcacc aagaaagtag atgaaaaagt caatccgctc
     2641 caaaagttca attgtgacgt actataatta cctaacgtat ctatgtaaag tgacataaaa
     2701 ggcatgacta aactaaatcc gataccagcc ataaatgttc caaaccataa gacaaacaag
     2761 ttttgttccc aagtttctct tttcctaaat aatttttcct ttattcttgc agccaattag
     2821 actccttctt tctgaaaatt ttcatctaca gctctttatt ataaactaca atatgtagtt
     2881 tatcaatgaa agtctctttt ttctctttct ttttcgtggt aaactgaaag agtgaaggat
     2941 ttattttatg agaggaaaca tggaaatgaa aaaaagaaat ctgtctcaag agaaaatcat
     3001 cgactgcttt cgagaactag cagaagagat ggatgttcag caaataacct ttcagcattt
     3061 agccaaacgc ttggatatca aggctccctc gctgtataac catttcaaaa atattcgtga
     3121 tgtcaaaaca gcgctaacag cgaaacttct gcaggaactt aatgaccagc tgcgacgggc
     3181 tcttgtggga aaaagtggtc aggaagcact gcagatctat gcagaaactt atcgatcctt
     3241 tgctttttca aatcaagcag tgtatgagtt actgatcagt gtaccgcata ccaatgaagc
     3301 tgttttatta gatggaatcc atgaaacgaa tcagatcatt ttgcagatat ttgaggcttt
     3361 tcaattaacg aaagaagaaa aagttcatcg aagcagggaa cttcgtagta tgatccacgg
     3421 ttatctttcc ttacgttttt taggatactt tacaaaagaa ccaacaatcg atccagaaga
     3481 cagctatttt tggatgatca ataactttat cgctactttg ccttaaacaa tcagattcat
     3541 ttgtaacaat tttaagatgt tcattaaatg acccttttat aagagaaaat tcttgcaact
     3601 ttcttgaaaa gtgactaaga tgagtctaga aaagctgaaa gaaggagcac gagtatgtat
     3661 caagtaatta ccatgttcgg ggacaatgag ccttggtggt tttttgaaga ctgggaagaa
     3721 gatattgaag aagaagaaac attcgagtct ttcgaagaag cacgacaagc gtacgaaaaa
     3781 caatggaacg aaatccatca aaattatgaa tatatcaatg caaaaagtaa ctttctcagc
     3841 gccttttgga atgacggaga tgagcgctgg tgtgaagagt gtgacgatga tttacagcaa
     3901 tacaagggac ttgcgttgtt gaaaaatcat cagcccatca cagtagaaag cagaaaggaa
     3961 ttttatgaaa caactaattc tagcggaaaa accaagcgtt gcgaaagacc taagcaaggt
     4021 gcttggtgct aatcaaaagc acaaaaatta ttatgaagga cccaaggtga tcgttacttg
     4081 ggcccttggt catttattag gattgaaaat gccggaagat ctgaataagg aatggcagag
     4141 ctggcaaatg gacacgttgc caatgatccc taaaaaacta ggaatcaaac cacttcctaa
     4201 aacaggacat caattaaaag caatcaagca gctggctcag cgaaaagatg tctcagaagc
     4261 agtcattgcg acagatgccg gaagagaagg ggaacttgtt gctcgctgga tcttagagta
     4321 tgtgcatttc aataaaccag tgaagcggtt atggatctct tctcaaacag ataaagcaat
     4381 caaaactgga ttcaagcaac taagacctgc gaaagaatat gatgcattgt atgaatctgc
     4441 tcttgcccgg gcaaaagcgg actggctggt aggattgaat gtgaccagag ccttaactgt
     4501 gaagtacgcg gataatttat cagcaggccg tgtgcagaca ccgacactag cattagtgag
     4561 agaccaagaa aagaaaatcg agcagttccg tccacaaacg tatttcacga tcacgttgca
     4621 agctggaaaa gaacaagcca aaatgcttca aaaaaatcaa tttgctttaa aaacacaaga
     4681 agaagcacaa aaacttgttc aattgatcag tacaagcaaa ggaactgtca cagaagtcca
     4741 agaaaaatta aaaacggaat ctgcgccatt gccttatgat ttaacagaga tccaaagaga
     4801 agccaatcaa cgctatggct attctgcaaa gaaaacatta agtcttgtac aaagtttgta
     4861 tgagacccat aagatcgtta cttatccgcg tacagacagc aaatatctga caaatgacat
     4921 gaaaagtacg atgaaagaac gattgcaagc agtcagcgac tttgctcctg aggtcaagca
     4981 gtatttgaaa aacggagcca tcgtccaaca gacaaaagta ttccaagaca gtaaagtgac
     5041 cgatcaccat gcacttttgc caactgaaaa cagagcacgt tatgaaaaat taagtaatga
     5101 agagcgaaaa atctatcaaa tgattgtctc tcgtttttta ggactgtttg ctcaacccca
     5161 taaagtcagc caaacaaaag ttacggttga atttggtaaa gaacgattca tttttcggca
     5221 aaatcgtgtc gtccaagcag gatggaaagg cgaaactgaa actgagacgg aaacagtcaa
     5281 atgggaaaaa ggaatgagaa tcaatcccga tttcgccatc aaaaaagaac tatctgcacc
     5341 gcctaaacca ttgtcagaag caagcttgct tggtcaaatg gaaaaataca gtctgggaac
     5401 cccagctacg cgtgcagaga ttatcgagaa actaatcaaa tctgaactga tggaacgtac
     5461 ccctcaagga ctgcaagtat ctcctaaagg taagcaatta ttagtattgg taaatccctc
     5521 tttagtaaca ccagatttga cagaaagctg ggaaaaagac cttgaagcta ttgctgcagg
     5581 aaagaaacaa gcaggaacct tcttgaatgg aatcgaaaaa gaaacaaaac gcttggtgaa
     5641 tgagatcaaa tcaagcaaac aagaatatca agatttctcg attacccaaa agaaatgccc
     5701 agaatgtggg gccaatctgc gcgagaaaaa cacacgtgac ggcaaaatct atgtctgcac
     5761 caattcagat tgcaactacc gcagaagaaa agatccaaaa gtctctaatc atcgatgccc
     5821 gcaatgtcac cggaaaatgg aaatcatcga aggtaaaaac ggtgcttatt tccgttgtaa
     5881 atacgatgga acgacagaaa aattgcttga taaaaaagag cagaagaaga aaatgaccaa
     5941 acatgaagaa cgacgcttgc tgaaaaaata tgcacaagca gaagaaccag aagaaagccc
     6001 attagcagca gcactaaaag cagcaatgaa gaagtaataa gatatgaaag aggttgtgat
     6061 agaagtggtc agcttcaaga aataagaagg aatttacgaa aattgcttct caaatttttg
     6121 tgaaatttcg gcttatttcc gaagaagcta cttctggaac accatttatt cggaaaaaag
     6181 gactgtgaca agacttttgt cacagtcctt tttcatttaa aaaaatgttt acttcatttg
     6241 acgtatatta ttggtgaggc ttcaattcat ttggcttcac tttttctgtt tctcttatga
     6301 tataaacagg acgctgtttc gtttctaaga agattttccc aatatatttc ccgatgattc
     6361 caagacataa caattggatt ccaccaatga acaggaagat cgagaccatg gaaggccatc
     6421 ctgatgtagg atcaccaaac aacaatgccc gcaaaataat gaacaaaatt gcaataccag
     6481 aaccgacaca agacaaagcg ccgataaatg aagcaatcgt taacggtgca tccgagaagt
     6541 tcacgatacc atcaatcgag tagttgaata atttccagaa ggaccaagat gtttcgccag
     6601 ccacacgttc gcggttttca taagaaatat attctgtttt aaatccgacc cagctaaata
     6661 atccttttga aaatcggttg tattctgtta attctaggac ggcatccacc atttgacgag
     6721 tcatcaagcg gaaatcacga gcgccatcga ccatttctgt atcgctgatt ttattgatca
     6781 actggtagaa ttttttcgca aagaaactgc gtatcgcagg ctcgccttca cgagtcgaac
     6841 gccgggtgcc gacacaatcg atatcagaag tagtgatccg gttgatcatc tcaggaagca
     6901 gttcgggcgg atcttgcaga tcaacatcca tcacggtcac taaatctcct gtagaagctt
     6961 gcaatccagc gtacagagcc gcttctttcc caaaatttct ggaaaatgag acaaagcgga
     7021 cataaggata agctgaggct agcttcttga ttgctgctaa cgtaccgtct tttgatccgt
     7081 cgtcaataaa caaatattcg aactcatggg cacctttgaa tttttcaacc tcatcaaaaa
     7141 aaagaggaac agttttttct tcattaaaac atgggacaac aattgatact ttcatcgttt
     7201 ctatcctttc aatcgtatgt ttatatatgg tttacattca aaatttaata aaccttaaat
     7261 aacaccttta aaatttgatt ttacgtaaaa gaacaggtat tctttttgta aactaacgta
     7321 tttagtttac catgtttcct cggtttatca aacttttaag aacaattttt attagttggc
     7381 aagtagtttt ggatagagaa aaaggaggaa gttccagtct attgactagg agcaaacaaa
     7441 atgaaaatac acacacctat cggcttttta tggttagatg cagatgaatc cggattgact
     7501 gctgtatctt ttcagccaat ttcagaaagc gaatcagcga atcagcgaat tttaacgcgc
     7561 acacaagaac agtttgaaga atattttgcg ggaaaaagaa aagaatttga tgttccttta
     7621 tctataaaga gaggtacgcc ttttcagcaa aatgtctgga aagctttgtc gaccattcct
     7681 tatggagaaa tccgaacgta taaagaagta gccgttgctg ttgatagtcc gaaagctgtg
     7741 cgagcgattg ggcaggcaaa tcgaaccaat ccattgccaa tcgttattcc ttgtcatcga
     7801 gtgatcgggc aaaatggaaa actgacagga tatatgggaa acgcaataga tggggttgaa
     7861 atcaaacgcc aattgttgtt gttagaagga tatctgcaaa attaaaaaca gttgtgattc
     7921 ggacattgtt ccatgcgaga atcacaactg ttttttcatg ttcattctaa actttgatca
     7981 gccaaataaa tagtaaagcc atcactgttt ggagaagccc gacagctaat ccataagata
     8041 agaagcgtgt accttctttc atgaattttg caaagtctaa acgcagacca attgccgcta
     8101 aagcagtgat ctcaaaccag ctgctgataa aatgtgccga ttggtcaata actacaggta
     8161 aagaaaacaa gctgttgacc aaacaaaaga tcacaaaccc taatacatac caaggaagcg
     8221 gtaattttcg ttttttctgc gtatcagttg gcgtttcaaa cagagctggt tgtttcttgt
     8281 gcatgaattt ttcaaacaga tagacaacaa caacgagcat cataatacgt agaatcttaa
     8341 atagcatggc gaattcgaca acagaattgt caagtaagct tgccccagcg actacttgac
     8401 caacagattg gagtgtacca cccaaaagtg cacttttttc aatgagattt ccaccaaaca
     8461 actgtaatcc aagaaaaggg agagtcaaca tcatcacagt acccagtaga ttgactagtg
     8521 tgatgatctg tcctttttct tcctctttcg catggatgga aggggcgatt gaagcaatag
     8581 cagaagaacc acaaacagcg tttcctccag ccatcatcaa agaagaagta tcgctgaatt
     8641 gcagtttttt accaatcaag taagcaccga tgatagtgac tgacatttgc agtaacacaa
     8701 aaacaattcc ttttacacca atttgagcaa tcgtttgagc tgtgacagtt gcacctagta
     8761 aaaaaacaga gaattctaat agtttgcttt cagcaaattt tgtgccgatt gctagaacag
     8821 gctgtttaaa aaaagtattt cctaataaaa tacccaataa aatagcaata gtcgctccac
     8881 ctaactgtgg cagataaata gcaagaagcc tgctgaaggc agcgatggca aaagaaagca
     8941 gtaaaccggg taagatttcg cggaccgctg aatcctttac agattttatt ggatgtgaca
     9001 atgatttcat ttgatcggtt cctttcgtca ataaatacta aaagcatact cgaacatagt
     9061 agcactaaac cagtattttg acaaaagaac aagaggtcgt gacaaaagtc ttgtacgacc
     9121 tcttgtttcg aataaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca
     9181 caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct
     9241 tttacgacct cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
     9301 gtacaaaaac atgaacagct gtttttgttt ggccgttctt attacttgat gcagaacagc
     9361 tttttcacaa cctattgaat cgattattaa gcgatttttt tagaaacaaa ttttccatgt
     9421 ttgctatgtt ctttaattaa gaaacgtacg taatgagcaa gaacgtagaa gacaactaca
     9481 cttgcaaaca gacattccgc aacttttaag aaatccaata cgctactata gatacctaaa
     9541 ctgttgaagt aatgaacagt agtaaatata gctgttgcag agataacaag cgggaatgta
     9601 aaggctgcat agcttggata aaaacgaagc ttgaacaaac gtggaagcat agacagcaca
     9661 acgaaataca atacttgaga taaagcgaac aatgctacga caagataaaa attaggttca
     9721 gcaaagtttt taagataacc tgttaggcaa agagaaccag gtgctgcaac gatcgtaatc
     9781 agcggacgtg tcggatgagc catatttctt acgaaaaaga cacgtttgat gatgatcggc
     9841 aataagaacg catagcagat caatgaaatc caaaagaaaa tttttccaat aagtgggaag
     9901 aagttcccgg cagttacagt gatgacccca aatccaacgt aaatgataaa ccagcttgga
     9961 taaattgctt caattgaaag atcagctttg ataacatggt gataattgaa ataaaagaca
    10021 aggatcactt ggaagataac agcgcacagc caaatgaatt tgacaattgg tgcaatactt
    10081 tcatatgaaa caaaataagt acaaataacc atgagtgaca tcgtaaaagt tggtgaaacg
    10141 gaggcgatga ttggatcatg caacgtttgt ttcgtgtgtt cgaataagat aaacaattta
    10201 acaaaaatga atatcataag aatcatagag ataccgccag ttagattgcc taaagcaaca
    10261 tggtgatagt ctttaaacag atttccaagg gaagctaacc caaggatcaa cccgcagatc
    10321 gggatcggaa taattttcaa aaagttcttc atatataata ttctcctttt tatttaatag
    10381 attttagtac caagatgaac gaatacattg taaaatgaat aggaatgtat gattacgagc
    10441 aagcagacag ctgattgtct tcatctaagt tactagttgg atcagcaact gttttgttgt
    10501 acatttctat tatagatggt tttttgtata tttaaatcaa atagttttta tagtaattat
    10561 cataaaaagt tatggaagtg gtgccatgtt taagttatta acgacttttc gagcagtata
    10621 tgaaacaaga aatttttcca gagcagcaga agtactgttt ttatctcaac cagcagtctc
    10681 gaatcaaatc aaacagttgg agcgggaatt gaatatcgat ctttttcaac gtaacggaag
    10741 aaaagagatg cttccaacta agcaagccga cttattatat gaacggacac tggttttgtt
    10801 agaagaatgg gaagaaactc agcaaaaatt gctcaatgaa caaaatgaag aagaagtatg
    10861 tcggatcgtt gcttcccaca cctttgctgt ctatcttttg cctcagttga tgaaacatct
    10921 gatcaaaagg tttcctaaag tgcattttga gatcgaaatc acgaattctc atgaatcatt
    10981 agaacaagta gcgaaacatg aatccgattt tggttttatc gaaaagccat tagagacttc
    11041 cggcgtacaa agatactctt tgatggaaga ccagctagtc atcgctgggg acccaaaaag
    11101 caaattgtgg cttgtaagag aagcaaactc tggtgttttt cattacacgc agcgttattt
    11161 tgaagagatg aatatccaag ataagaagat gatcgtgaaa agtaacgatg taattgttgc
    11221 gttgctgaag gaaggaatcg gtcgctcgat tttgtctaag cgctctgttc cagaaaatgt
    11281 gccaatgact ccgttgaatt tccatcgata tttttatttc atccaacgaa ccaatctggt
    11341 ttctgatcag ctgctagaga ttgccgaaga gatccttcga tacaatgaaa gcacagaaac
    11401 ttctcacagc gaggagaatc tcagctggta gataattgca tgtatcaaaa aaagttctat
    11461 gatacactaa agataagcag taggcttttg tactgcattt gctcaagcaa ctgcacaaac
    11521 ggaaagttaa tttttcccaa atgtctatgg aaagactttc cattatctaa ctggaccgct
    11581 ataataatca gcagtatggt ttttaagaaa agggatgaaa catggaaaaa aaattaaaag
    11641 cttcgaacga taatcgtatt gattatggcg tgattttacc agtctttctg ttatgtttga
    11701 ttggtctgct ctcattatat gtagcactta gccatgatcc gaacaatcca gcagtcttga
    11761 aaggcgtagg gatgcaagtt gtctggtatt tagttggagc ggcagcaatc gttgtcatca
    11821 tgcatttgaa ttccaaatgg atctggaaac tgactcctta cctttatggt ttggggttag
    11881 tggtaatggg actcttgctc aaattctatg atcagaattt agctgcttca acagggtcta
    11941 aaaactggtt tcgattcggt gcgtttactt tccagccagc agagttgatg aagattgcgt
    12001 acatcttgat gatggcactg atcgtaacta aacataatac gcaagtaaaa gaacggacga
    12061 tgaaatcgga cttttggctg attgggaagc tcttgatcgt gacaataccg gttttagcat
    12121 tgatcatggc ccaagatgac tttggaacga tgcttgtctt tcttgcgatc tttggcggga
    12181 ttttcctgat gtctggaatc tcttggcgca tcatcgcacc tgtagtaatc ttggcagtag
    12241 tcgttggagc aggaacgatc ttcttggtta cgactgaagg cggaagagac ttgctttata
    12301 aagttggatt caaatcttat cagtttgcac gtattgattc atggctggac cctttccatg
    12361 atacgtcagg aatgagttat cagccggccc aaggactttt ggcaattggt accggtggct
    12421 tgtttggtaa aggattcaac gtaagcaata tctatgtgcc tgttcgtgag tccgatatga
    12481 tttttaccgt tatcggtgaa aacttcggct ttatcggtgg tgcattcgtc attttcttgt
    12541 attttatttt aatctatcgt atgattcggg tgtgtttcga tacgaataat gaattttacg
    12601 cctatatcgc aagcggtttg atcatgatgc tgctgttcca tgtctttgag aatattggag
    12661 caaatatcgg attacttcct ttaacaggga tcccattgcc tttcattagt cagggaggat
    12721 cctcgatttt agggaacatg atcggtatcg ggttgatatt atcgatgaga taccaaaatg
    12781 aagcacctgc aagatcttca aggaggtaag aaaattgtac accttttact ggtacccgaa
    12841 atgttcaaca tgcaaaaaag ccaaagcgtg gttagaccag catggcgtta gttatcaaac
    12901 cgttgatatg atcaaagaag taccgaaggc ctcaacgatt gagacttggc tagaaacgag
    12961 tcaagtacca atccgtcgcc tgttcaacac aagcgggatg aaatatcggg aacttggact
    13021 gaaagatcaa gtaggcgagt ttacggcttc aaaagcgagc caagtcttat cgacagatgg
    13081 tatgctgatc aagcgaccac tgatcttacg agaggaccag cttgttgcaa ttggattcaa
    13141 tgaaaaaatt tatgaaggag aactgatata aaatggcagg caagcatttg aagaaaaagg
    13201 acaacctttg gatcttattt aatggaaaag aatactgtgt tggtttgaca aatgaagcgc
    13261 aagaagaact tggagacatc acgtttgcca atctgccaaa agcaggacaa tcgctaaaaa
    13321 aagatgaacc tctgatcgaa gttgaagcgg aaaaagcggt caatgaattt gccagcccgc
    13381 taacgggtac agtttcttct gttaatgata aaatcggtga agatatcaat atcttgaatg
    13441 attcagatga aatgaatgcg tggatcttaa gtttcaaaga tgttgatcca aaagaatttg
    13501 acgaattata aaatcagaaa atcacttgaa aaaagttgac actgattctt ccagttgcta
    13561 taatcataag caatcctata tgaaacgaga aagctttgaa aagaagagta catgtcacgg
    13621 acgtttgaag agagccctgt ttgctgaaaa tgggtaacga aaatgagatg gaagatggtc
    13681 ttagagcaga gtaagcgagc gtaagcaaac ttactacggg agtgcccgtt acagcgcgac
    13741 agtatacgga aagaagctaa gtccaagtac tggaagaggc tgttattgcg aagtaacggt
    13801 aaatcaaggt ggtaacacgg aagttcaagc tttcgtcctt ttgcttaagg caagagggcg
    13861 aaggcttttt tgtttgaaaa attataagag aaagatgtga atggcatgtc attaatccaa
    13921 ctaacgaatg tcaaaaagac attcaatggg aaaaacggaa acatccaagc cgtcaatgac
    13981 gtttcactaa acgttgaaaa aggggatatt tatggaatcg tcggctattc tggcgcagga
    14041 aaaagtacat tagtccgtct cctgaatgga cttgagcttc ccacgagcgg cgaagtgatc
    14101 gtaaacgatc aagatattac aaaattaaaa aacaaagaat tacgtacatt ccgaaagaaa
    14161 atcggtatga tcttccagca tttcaacctg ttatggtcaa gaactgtact tgaaaatatc
    14221 cagctgccgc tggaattagc tggcgtacca aagagcaaac gtaaagaacg ggcagaagaa
    14281 ctgttgcgtt tagtcgggtt agaaggacgc ggacaagcat atccttcaca actttccggc
    14341 ggacagaaac aacgcgtcgg tatcgcacga gcattggcaa atgatcctga gatcctgctt
    14401 tgtgatgagg ctactagcgc attggacccg caaacaacag aagaagtatt agacttattg
    14461 ttagcgatca ataagaaatt gaatttgacc atcgtcttga tcacacatga aatgaatgtg
    14521 atccgcaaaa tctgcaacaa agtcgctgtg atggagttgg ggaaagtcgt agaagaagga
    14581 gacgtattga ctgtcttcag acaaccgaaa aaagaagtaa ccaaacgctt tgtacaacaa
    14641 gacatcgaac cagaagctga ttcgacagaa ctattagctg aactcatcaa agaaaatcca
    14701 agcggccgtt tggcaacttt gacctttagc gaaagaaatg ccaacgagcc agtcatttcc
    14761 caagcgattc gacgctataa catcgatgtc aacgttgtgt atggaaagat caaacaagtg
    14821 aaagaaggtt cctttggttc attgactgtc ttgatgactg gtgaaaccaa agaattagat
    14881 caagcagaag tgttcatcaa agaacaagga gtaggaattg aggtgatcca tcgtggataa
    14941 aagcttttca gaaacatatc tagattttaa acaaatcaac ccggaaacga tgaatcaggc
    15001 tatccttgat acactatata tgacattaat ctcaatggtc ttagtgacga tcatcggatt
    15061 agttttggga ttgatccttt actctattgg acgcaaaaac aaaccgtatg ctcgaatcat
    15121 ttatggaatc gtatcgatca tcagtaatat tttccggtca acgccattta tgatcctgat
    15181 ggttttgatc attccattta ctaaagcaat cgtaggcact atgctaggtg cgcaagcagc
    15241 cattccagct cttgttttat ccgcagcacc attttatgcc cgtctagtag agattgcttt
    15301 tcgtgaagtc agccctggtg tactggaagc agcagatgca atgggagcaa gttactggga
    15361 aatcatctgg aaggtcttga tacctgaaag tattcccgca ttgatttctg gattaacagt
    15421 tacaacgatc tcgatgatcg gatttactgc gatggccggc gcgatcggcg caggtggact
    15481 tggaggtctt gcttggcagg aaggctacca gcgtggtaat ctaacagtga cattcgttgc
    15541 gactttaatt attttactaa tcgtatttat cgttcaagga atcggtgatt tcttaactaa
    15601 gaaaacagac aaaagataat aggatatagg gggatgtagg atatgaaaaa gaaaattttt
    15661 ggatttgcag cagcgctttt gctgactgta ggattagcag catgcggtaa caacagcgac
    15721 agcaaagatt cagcaaataa agcagacgac acgacattga aagtcggcgc ttcaccgaca
    15781 ccgcacgcag agattttgga acatgtgaaa ccattattga aagatgaagg aatcgatctt
    15841 gaaatcgtca aattcgatga ctatgtatta ccgaaccaat ctttggaaga aggcgacatt
    15901 gatgccaatt acttccagca tattccttat ttgaacaaag aaatcaaaga aaaaggatat
    15961 gactttgtca atgccggtgc tgtccacatc gaaccaatgg gcttgtactc taaaaaagtc
    16021 aaagatgttt ccgaattaaa agatggtgca acaatcatta cttcaaattc tgaatcagac
    16081 tggggacgca tcatcacaat tctacaagat gctggcttag tcaaagtcaa agatggagta
    16141 gatctggaaa cagcaacatt cgatgatatt gaagaaaacc ctaagaactt gaagttcaac
    16201 catacgatcg acccatcact tttagcttct acgtaccaaa atgatgaagg tgacctagtt
    16261 gcaatcaacg caaacttcgc ttacggcgca gggttgaatc cattagaaga tgccgtattg
    16321 cttgaaaagg acaactctcc ttacgtgaat attattgcga ctcgtaaagg tgatgaagac
    16381 agtgacaaaa tcaaaaaact gattgaagtg cttcaccgca aagaagtcca agaatggatc
    16441 acagacaaat ggggcggctc tgttaaacca gtaaaggctg atgcaaaata aggtatggaa
    16501 caaaaaagat gagaaataag tctgtaatgg cttatttctc atctttttta tacctattct
    16561 aatttagaaa tcaaaattgt ttaataataa ataaaagata tttgatttta gtaaggaagg
    16621 tcattgcgct tcattctcaa ttagtctaaa atgagtgaga acgaaagaga atttgggggt
    16681 ataatgcatg tctgtcttag aaataaaaaa tctacatgtg tcgatcgagg aaaaagaaat
    16741 ccttaaaggc gtaaacctaa caatgaaaac cggagaaatc catgccatca tgggacctaa
    16801 cggcacagga aaatctacct tatctgccgc tatcatgggc aatccaaact atgaagtaac
    16861 agaaggcgaa attttgtttg acgggaaaaa tgttttagac ttggaagtag atgaacgtgc
    16921 gcgacttggc ttatttttag ctatgcaata tccaagtgag ataccaggta tcacgaatgc
    16981 ggaattcatg cgtgcagcga tcaatgcgaa acgtgatgaa aacgacaaaa tttctgttat
    17041 ggcatttttg aaaaagttag atgaaaaaat ggctttacta aatatgccag aagaaatggc
    17101 tgaacgttat ctgaacgaag gtttttctgg cggagaaaag aaacggaatg agatcctgca
    17161 attgttgatg ttagaaccta catttgcaat tttagacgaa atcgattcag gtctggatat
    17221 cgatgctttg aaagtcgttg ctaaaggagt aaatgaaatg cgcggagaca atttcggtgc
    17281 attgatcatc acccactatc aacgattatt gaactatatc acacctgatg tcgttcacat
    17341 catgatggaa ggccgagtag tcatgacagg gaatgctgac ctagctaaac gtctggaagc
    17401 agaaggctac gctggtatca gtaaagaatt aggaatcgac tataaagaag aagaagcgta
    17461 agggggagca agaatgaaag aaaaaaactg gctcgattat cttgacgcgg taaatgattt
    17521 ttctttaagt aaaggcgaac ctgattggat gagaaccttc cgtcaagacg cactagcgaa
    17581 agcagatgaa ctgccattgc cgcatatcga tcgggtgaaa ttccatcgct ggtcgttatt
    17641 tgatgtgaaa gaaacacaaa ccatcagtga aacaggcacg attccggcat ttgatgcaat
    17701 gaaagacaat cctgtgcttg tccaacaagg ttcatggacg atttttgaac aactgcctgt
    17761 ggaattagca gaaaaaggag tcatttttac cgacttgttc actgcaatga tcgaatatcc
    17821 agaactagtc caagaatatt atatgaaaaa agcagtaaac atgaacgaag accaattgac
    17881 tgcactccat gtagctttta tgaacagcgg tattttcctg tatgtgccta aaaatgtcgt
    17941 gatcgatgaa ccactcgagt ccttattcat tcaagatggc gcatccgatg agcacttctt
    18001 caagcatgtg ttgatcgtgg cagatgaaca tagtgaattt tcttatctgg aacgtttcca
    18061 gacaaccaaa gaacaagtag caaaatcatc aggtaatatc atcgtggaag tcatcgctaa
    18121 agctggttca aaaatcaaat attcagccgt agatcaatta ggagaaaata ttacttctta
    18181 tatgaatcgc cgtgggcaca ttttacgaga tgcttctgtc gactgggcaa ttggtgtcat
    18241 gaatgacggt catgtgatcg ctgactttga ctcagattta gcaggagaag gtgcacacgc
    18301 agaagtcaaa atcgtggcaa tcagctcggg cagacaaatc caaggaatcg atacacgagt
    18361 aaccaataaa gcccctcata cgatcgggca tattttgcaa cacggagtga tccgagaaaa
    18421 aggaacattg acctttaatg gtatcggcca catcttgaaa ggggcaaaag gtgcagatgc
    18481 ccaacaagaa agtcgtgtct tgatgctttc tgataaagca cgtggtgatg cgaatcctat
    18541 tcttttgatc gatgaaaacg aagtaactgc tggacatgct gccagtgtag gacgagttga
    18601 tccagaagaa atgtactatc tgatgagtag aggattgcat aaagaagaag cagagcgctt
    18661 agtgattcga ggattcctcg gttctgtctt aacagcaatc cctgtagaac aagttcggaa
    18721 agaattagtc gaagtaatcg aagggaagtt gaatggatga tcgatgctaa aaagctacgc
    18781 aatgattttc ctatcttatt tcaagacgtc aatgatgaac ctttagttta tctggataat
    18841 gcagcgacga cccaaaagcc aaaacaagta ctccaggcat tagcgtacta ttacgaacac
    18901 gataacgcca atgttcatcg cggtgtccat acgttagcag aacgcgcgac ccatgattat
    18961 gaacaagcaa gagaaaaagt acgggcgttc atccatgcaa aagaaacagc agaagtgctg
    19021 tttactcgtg gtacgacgac aagcttgaat tgggtagcca gaaccttcgg cgaccgctat
    19081 atcaaagcag gagacgagat agtgctctct tacatggaac atcattctaa tgttatcccg
    19141 tggcagcagt tggcaaaacg aacaggtgct gtcttgaaat atatcgaact aacagaagaa
    19201 ggcttcctgg atatggagga tgcagcgaaa aagattacgg acaaaaccaa aatcgtctcc
    19261 attgctcatg tatccaatgt cttaggtgtc ttcaatcctg ttcaagaatt agctgaactt
    19321 gtccatgaaa acggcggggt catcgtagta gatggtgctc aatctgcccc gcacatggcc
    19381 attgatgtcc aagcactaga ctgtgatttc tttgctttca gcggacacaa aatgtgcgga
    19441 ccaacaggta ttggtgtttt atatggtaaa cgaaaatggt tagaagaaat ggaacctgtt
    19501 gaatttggtg gcgaaatgat cgattttgtc gaattatacg acagtacatg gaaagaacta
    19561 ccttggaaat tcgaagcagg gacaccaaat atcgccggcg ctatcgcatt aggccatgca
    19621 atcgattatt tgcaaaaact cggtatggac aatatccatc gatatgaaga agaactagcc
    19681 gcttatgtgt tgccaaaatt acaagcaatt gatggattga ctgtttatgg tccacaagac
    19741 ccgaattttc ggacaggagt cgttgcattc aatttggatg ggttacatcc ccatgatgtc
    19801 gcgacagctt tagacatgga aggggtagcc gtcagagcag gtcatcattg tgcgcaaccg
    19861 ctattgaagt accttgaagt atcagcgact gctcgcgcga gcttttattt ctacaacaca
    19921 acgcaagacg ccgaccgttt agttgaagca atcatggcaa caaaggagtt tttccaacat
    19981 gggactatct aaattagaca atttataccg tcaagtgatc ttggatcatt cttcccatcc
    20041 gcatcatcgt ggaagcttga ctgactcgac aagcaagatc gaattgaaca accccacatg
    20101 tggagatgtg atcgaattgc aagtcgatgt aaaagacgga atcatcaaaa atatcgcctt
    20161 tgacggcagt ggctgctcca tcagtacggc aagtgcctcg atgatgacag acgccgtcat
    20221 cggaaaatca atagaagaag cagaacattt aactgtagac tttctacagc ttgtccaagg
    20281 aaaagaagtc aaagatcccg accaattagg cgatgctgct atgcttggtg gtgtagctaa
    20341 atttcccgct cggatcaaat gtgcgacact tgcctggaaa gcactaggaa aagcaatgga
    20401 aaacgatggt accgcagctt tccatcatta ccacaaggaa gaggagtgag aacatgggcg
    20461 taccagaatt agaagaatat aaattcgggt ttcacgatga tgtcgaaccg gttttcagta
    20521 caggagaagg cttgacagaa gaagtgatc
//
LOCUS       DAYLZO010000046        20480 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.047, whole genome shotgun sequence.
ACCESSION   DAYLZO010000046 DAYLZO010000000
VERSION     DAYLZO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.047"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..1835)
                     /locus_tag="ACGVJG_001512"
     CDS             complement(<1..1835)
                     /locus_tag="ACGVJG_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFJ6557460.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACGVJG_001513"
     CDS             complement(1832..2536)
                     /locus_tag="ACGVJG_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFJ6557461.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACGVJG_001514"
     CDS             complement(2548..4857)
                     /locus_tag="ACGVJG_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFJ6557462.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACGVJG_001515"
     CDS             complement(5052..5516)
                     /locus_tag="ACGVJG_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557463.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACGVJG_001516"
     CDS             complement(5516..6142)
                     /locus_tag="ACGVJG_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFJ6557464.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACGVJG_001517"
     CDS             complement(6149..6514)
                     /locus_tag="ACGVJG_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFJ6557465.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACGVJG_001518"
     CDS             complement(6504..6842)
                     /locus_tag="ACGVJG_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557466.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACGVJG_001519"
     CDS             complement(6832..7158)
                     /locus_tag="ACGVJG_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFJ6557467.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACGVJG_001520"
     CDS             complement(7136..7417)
                     /locus_tag="ACGVJG_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFJ6557468.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACGVJG_001521"
     CDS             complement(7419..8783)
                     /locus_tag="ACGVJG_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFJ6557469.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACGVJG_001522"
     CDS             complement(8796..9365)
                     /locus_tag="ACGVJG_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFJ6557470.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACGVJG_001523"
     CDS             complement(9337..10524)
                     /locus_tag="ACGVJG_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFJ6557471.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACGVJG_001524"
     CDS             complement(10588..12315)
                     /locus_tag="ACGVJG_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFJ6557472.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESSFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACGVJG_001525"
     CDS             complement(12312..12764)
                     /locus_tag="ACGVJG_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFJ6557473.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACGVJG_001526"
     CDS             complement(12876..13256)
                     /locus_tag="ACGVJG_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFJ6557474.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACGVJG_001527"
     CDS             complement(13253..13639)
                     /locus_tag="ACGVJG_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFJ6557475.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACGVJG_001528"
     CDS             complement(13641..13919)
                     /locus_tag="ACGVJG_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557476.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACGVJG_001529"
     CDS             13967..14695
                     /locus_tag="ACGVJG_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFJ6557477.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACGVJG_001530"
     CDS             complement(14841..15317)
                     /locus_tag="ACGVJG_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFJ6557478.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACGVJG_001531"
     CDS             complement(15277..15633)
                     /locus_tag="ACGVJG_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557479.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACGVJG_001532"
     CDS             complement(15611..15796)
                     /locus_tag="ACGVJG_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557480.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACGVJG_001533"
     CDS             complement(15797..16069)
                     /locus_tag="ACGVJG_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFJ6557481.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACGVJG_001534"
     CDS             complement(16066..16266)
                     /locus_tag="ACGVJG_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557482.1"
                     /translation="MKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACGVJG_001535"
     CDS             16394..16606
                     /locus_tag="ACGVJG_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557483.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACGVJG_001536"
     CDS             complement(16639..16833)
                     /locus_tag="ACGVJG_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557484.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16841..17029)
                     /locus_tag="ACGVJG_001537"
     CDS             complement(16841..17029)
                     /locus_tag="ACGVJG_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557485.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(17026..17166)
                     /locus_tag="ACGVJG_001538"
     CDS             complement(17026..17166)
                     /locus_tag="ACGVJG_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557486.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17656..18021)
                     /locus_tag="ACGVJG_001539"
     CDS             complement(17656..18021)
                     /locus_tag="ACGVJG_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557487.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18061..18363)
                     /locus_tag="ACGVJG_001540"
     CDS             complement(18061..18363)
                     /locus_tag="ACGVJG_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFJ6557488.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18363..18662)
                     /locus_tag="ACGVJG_001541"
     CDS             complement(18363..18662)
                     /locus_tag="ACGVJG_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557489.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18655..18819)
                     /locus_tag="ACGVJG_001542"
     CDS             complement(18655..18819)
                     /locus_tag="ACGVJG_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557490.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18825..19094)
                     /locus_tag="ACGVJG_001543"
     CDS             complement(18825..19094)
                     /locus_tag="ACGVJG_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557491.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19106..19855)
                     /locus_tag="ACGVJG_001544"
     CDS             complement(19106..19855)
                     /locus_tag="ACGVJG_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557492.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19858..>20480)
                     /locus_tag="ACGVJG_001545"
     CDS             complement(19858..>20480)
                     /locus_tag="ACGVJG_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFJ6557493.1"
                     /translation="ATINEEVNIERLKTMYDGYDGDRIIEVRFIDPRRFTVQQRNFIY
                     ALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLKDESNTTVSDANVLANIILDFIF
                     EHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHS
                     EYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQKTIKKLNIGG"
BASE COUNT         5678 a         4332 c         3194 g         7276 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgacgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattatcttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa tttttttctg
    16861 ctagatggag caaaagcaat ccgacatcaa gaatcttttg acccattatt tcagctactt
    16921 tgctcggctt aactccttga gaaaagagca agcgtgcttg ctctatctct tcgtgagtcc
    16981 aaataaattt ggcttcctca aggataatta ctggatcagt tgctctcatt gttcctctgg
    17041 aaaaatgatg tcattcttat gcttggacca ggtatctgca tatggaacaa agtattgtct
    17101 agctaattcg atttgatcaa ttaaagcagt ttctgataat tcatggtctg cagcaatttc
    17161 attcatacta tcgccttcat ctaaacagat aagaccgttg acgttatcga tttttacatt
    17221 tgtgactgct tgactttctc cacgctcata atattctaca gttgatactg atacatcagc
    17281 ttcggagttt aattgcagaa tctgttactg ccgggataat ttaatattct tttcataatt
    17341 cacctttttt cctactgaca tcgaatattt ttcatgttat gattagccgt gaaaggagag
    17401 ctatattatg tgtttacaca tgatgtcctt agctcattcg gttctttgga agcttttttg
    17461 gaaataactc tcaggttctt gacgcaaatc tcatttattc tttgaaacaa aaaacttcca
    17521 ttttccgaga gctttggatc acttcaaaag agatttgcat ttatatttct gtgaccttga
    17581 gtcgcgtaaa gaaccttaca gcaatggcgg caattccact ttcaggaaac cgcctttttt
    17641 cattgatatt ggtggttagc ggactaccca ttttctttcg gcagtgccaa accacaaatg
    17701 atctgtgttg atgtttaggt agttgtatac cagataagct aaatccaaag tgcctgcatc
    17761 ttcaagacga caatattcca aaaattctcc catagctgga cataaaaatc ctggagctat
    17821 catatcccaa cagttaatag tgcaaagaaa ttcgtttagc ttctgaacgt tttctttggt
    17881 ttcacgaatt acaacatact ccaattcttc tccgctggca gagcattcca aaacttcaaa
    17941 ttgattaaga aatggtagaa catatgcttc aaacatcaac attccatctt tctgtttttg
    18001 cacttgtttt gcatcaatca tgtctatcct cctattcagt tatttcttcc attaatggaa
    18061 ttatttcagg tcactggact taacgaaaac accattgacc atctttccag tccgaccttt
    18121 gatttcgtca taagcaaaat ccaagcactc gtataaatcc atatcatttt gcattgccaa
    18181 gatgatcaag gttactacta catcgccaat tccgtctctt aaaccatgtt catcttttct
    18241 agctagagaa gcggcaactt ccccgatttc ttcgaccgtt tttaacattt gcttgctgaa
    18301 atccgcttga tccaatccct tatcttttgc ccactgctct actttcgcga ttagttcgtc
    18361 cattatttct cctccacata cctaaactgt cttccttttg aatcaatcca taagctccta
    18421 gctctatccc aaataatatt tttgctcaga ccagtaattt cagataactg tttagcggta
    18481 cccgttacta gaattcgatc accatgccag attgcaatct ttctcggcgt tctccgtttg
    18541 ggcttttcag tccacattga tttaccgagc ttttggactt ctgcaactat ttttttatct
    18601 tcctgccaat cttctgaata agtcaattcg ataattcgct gcattgccgc tttcttatcc
    18661 atcccgacat tctcctttca gtaatttgag tacttgatca agtgcgctct cacgtccgcc
    18721 atgaaatgtg ttgagccact tgtcttcgta cgacacactt tgtcttaaag cttcttgatg
    18781 cattaattcg atctgtgctg taaatgtctt taggtccatc ttgtttacac ctgctcaagt
    18841 tcattaagat gtttttgcaa gcctttaaca caatcaatga atagtaattt ggtatatgct
    18901 aaatttctta attgcgttcc atcgatatag agtgcgaaat agtatctgag tttactccaa
    18961 ctcgaacgat cattcttaat tccttcaatt ccagcttctt cgagttgatc atatacgtct
    19021 ctcaggacgt ctaattcctc gcccttttta taattcgcta tttcattaat tagttctaga
    19081 taatcgatct tcatttttcc acctcttaga atggtgcttt tgattgtcta ttagctcgtt
    19141 ctagcgcttt tttcttgaga taggcttctt ggtcgattgc ccattcagga agcttctctc
    19201 gtcttcctgt gcgcttgtat ccactgcttg cgttcttagg ttcacttttt tctttccttg
    19261 cccaacttcg aatagttgcc aaatagtttt tataagtctt accagatgat tcacaatact
    19321 ctgacagtcg ttcgattcgc tcttggtaat cattagggaa ttctgttttg agtttctcca
    19381 tctgctcatc tgacaaaaga acatttttat actctccgta tttatgacgg acgggcttag
    19441 ccttcgattt tttcgaaggc ggtaactctc ttatatattc ttttgtatta ttaaatgtat
    19501 tattaataga tgtattatta tctttgactt tttcgtcaat aggggtattg cgtttttcgt
    19561 caatagggta ttgattaatt cgtaggtacc tattgattat ttgattggta ccctctttgt
    19621 aaatgatttc ccgattcaag tatccaaact taatcaaatc acttacccat cgcgatatgg
    19681 tctctttatt cacaccatat aaatctgcaa agtactcatt gcctgcccaa caaaagcctc
    19741 tttcattaca caaggccgtt atctctccgt ataacaactt agtatttggt ttaagtcttt
    19801 tgtcgtacct tacgttggct ggtataatcg cataataact tcgatgttct gtcattttta
    19861 ccctccaata tttaactttt tgattgtttt ctggtttaat ttgatccctt tgatttgata
    19921 tttatttttg aaattaatca cacctatttt gtgcttctct gtgtgatgga ttctgcagag
    19981 tgctgcaaat gtgtactctg aatgatcaac ttctttgcgc tttcgtcttc ctagcgcttt
    20041 gtcaaagtga tcgatgtcag ctcctgtttt gccacagatg cagcagactc tttttgtgat
    20101 gcatttgtag aagtaatatt cttgattcgc tggtaaaatc tcatagcctt ctttgaaagg
    20161 aatatgatgt tcaaagatga aatctaagat gatatttgct aagacattag catcactcac
    20221 agttgtattc gattcgtctt tgaggcttat tttgcgccct gtgacgcctt caaaacgaaa
    20281 gtagaagaat tccttccaga agtccgttgg catgcctgta tcgatgaaaa tatcgcctat
    20341 gagcgcatag atgaagtttc gttgctgtac agtgaaacgt ctaggatcaa taaatcgaac
    20401 ttcaataatc cgatcgccat catatccgtc gtacatcgtc ttcaaacgtt cgatgttcac
    20461 ttcttcatta atagttgcac
//
LOCUS       DAYLZO010000047        20367 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.048, whole genome shotgun sequence.
ACCESSION   DAYLZO010000047 DAYLZO010000000
VERSION     DAYLZO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.048"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1350
                     /locus_tag="ACGVJG_001546"
     CDS             <1..1350
                     /locus_tag="ACGVJG_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFJ6557494.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2815
                     /locus_tag="ACGVJG_001547"
     CDS             1484..2815
                     /locus_tag="ACGVJG_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFJ6557495.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFIFSFVKLS"
     gene            complement(2802..3761)
                     /locus_tag="ACGVJG_001548"
     CDS             complement(2802..3761)
                     /locus_tag="ACGVJG_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFJ6557496.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            4005..5408
                     /locus_tag="ACGVJG_001549"
     CDS             4005..5408
                     /locus_tag="ACGVJG_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFJ6557497.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            5757..6389
                     /locus_tag="ACGVJG_001550"
     CDS             5757..6389
                     /locus_tag="ACGVJG_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFJ6557498.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            6977..8416
                     /locus_tag="ACGVJG_001551"
     CDS             6977..8416
                     /locus_tag="ACGVJG_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFJ6557499.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            8711..8929
                     /locus_tag="ACGVJG_001552"
                     /pseudo
     CDS             8711..8929
                     /locus_tag="ACGVJG_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            9178..9393
                     /locus_tag="ACGVJG_001553"
     CDS             9178..9393
                     /locus_tag="ACGVJG_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557500.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(9610..9866)
                     /locus_tag="ACGVJG_001554"
                     /pseudo
     CDS             complement(9610..9866)
                     /locus_tag="ACGVJG_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10053..10964)
                     /locus_tag="ACGVJG_001555"
     CDS             complement(10053..10964)
                     /locus_tag="ACGVJG_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFJ6557501.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(10983..11987)
                     /locus_tag="ACGVJG_001556"
     CDS             complement(10983..11987)
                     /locus_tag="ACGVJG_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFJ6557502.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(11984..14107)
                     /locus_tag="ACGVJG_001557"
     CDS             complement(11984..14107)
                     /locus_tag="ACGVJG_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFJ6557503.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(14112..15053)
                     /locus_tag="ACGVJG_001558"
                     /pseudo
     CDS             complement(14112..15053)
                     /locus_tag="ACGVJG_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(15166..16992)
                     /gene="ltrA"
                     /locus_tag="ACGVJG_001559"
     CDS             complement(15166..16992)
                     /gene="ltrA"
                     /locus_tag="ACGVJG_001559"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFJ6557504.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            complement(17737..19239)
                     /locus_tag="ACGVJG_001560"
                     /pseudo
     CDS             complement(17737..19239)
                     /locus_tag="ACGVJG_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(19226..19615)
                     /locus_tag="ACGVJG_001561"
     CDS             complement(19226..19615)
                     /locus_tag="ACGVJG_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFJ6557505.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(19675..20178)
                     /locus_tag="ACGVJG_001562"
     CDS             complement(19675..20178)
                     /locus_tag="ACGVJG_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFJ6557506.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
BASE COUNT         6315 a         3887 c         3722 g         6443 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttttcgttt gtcaaattaa gttagacaaa
     2821 tcatcttttg tgacgtaact caaaaatact tccattggtg tccgatagtg taatgatttt
     2881 ctaggaatat gatttcgttt cgatgccacc gattggataa aggtttcaga tacaccatta
     2941 aaatccatag ctttatacaa accatcttta cgtaataagc catttgaatg ttcattcaat
     3001 cctcgttgag aaggtgttcc tggatccgca aaataaattg aaatatcgtt ggtattacta
     3061 atctttttcc aatttgagaa ttcttttcca caatcaaatg tgatggattt aaataagttt
     3121 tttgggacac aacgaaacca ttgattcagc ctattttcaa tgtctttagc ctgtctgcct
     3181 tcgggtttca aggtaatgat gactttagat agccgttcaa ctaatgtgat gaccgcgctt
     3241 ttatgttttc cacccacgat agtgtcacct tccaggtgac caaattcatg atcaaactgg
     3301 gtatagtctg tctttctgtc tcggattgac cggcgaaacc cttgttttcc tcttttttct
     3361 ttatgaccgt ttggttttct ttttcccttc attggtaaat cgtgagtgtc aaaggtttta
     3421 tctttaaaca ttcgatacag tgtgcgtacc gaacaagcaa taggaaactc tgctcgacca
     3481 ataaggacat caggagccca cccttgaaca accttttttt gaatgtattc ctgttgctca
     3541 tcaggtaaaa caatcggatg tcttccgcag ttacgtttgt ttttcttata ttgtttatag
     3601 tagctcattg cgcttccacc agatttaaga aaacgataga ccttataaat agtttgtcga
     3661 gaacgttgta acagattggc tacttttgaa acaggttgtg attgattgaa ataagcttct
     3721 atcattacga gttcagttgg tgtaagatgg ttataggtca tttgtactca ctccttataa
     3781 ttctttcgtc ggaactattt tgagtgtagc acaaatgact ttttttagtt gtctagctta
     3841 attttacaat cggcgtttta aaggcagtaa caggattaaa cattgaaaca taatcgttac
     3901 taaatgaaat caagaaatat actgtattac tcaattcatt gaaaatattt atcataattt
     3961 ttaatagaaa acactattaa agagaaaaaa gagaggtaaa tcaaatgaaa aaatctttaa
     4021 aacttgcatc agcagttata ttaatgtccc aactccttat agcaacacct ttccatgtgt
     4081 tggcaagcga aaattcaaca tacaaaattg caaaagaaag aaccgagacg actgaaatga
     4141 gtaattccga tgtaacagca gagagtagtg aacttttgac aacttcatca agtgaaagta
     4201 gtacgacttc aaatgccgag tcttcaacaa cttcatcaag tgaaagtagt acgacttcaa
     4261 atgccgagtc ttcaacaact tcgtcaagtg aaagtagtac gatttcaagt accgaatctt
     4321 caacaacttc aagtagtaca agtgaaataa cttcttcgaa agtgacaaaa ccaaaccata
     4381 tagaatcttc aaaaaaggaa gcgccaaaaa cggaaggaca aacaaaaact ttttcgacac
     4441 cacagcaaat agaatcaaca gaatctttcc aatcacaacc aatactatct ggtaatgaag
     4501 aaattcattt tgacaaaaat gaatctgtag aaaattttat tatgaaaatt ggtgagtctg
     4561 cgagaaaaat tggtcagaaa tatgatttat atgcttctgt tatgattgca caagcgatct
     4621 tagaatctgc cagtggtcaa agccagttag ctcaggcacc gaattacaat ttatttggga
     4681 taaaaggaac ttataatgga aactttgtca ttatggtaac aaacgaagat ttgggtaatg
     4741 gaacgcttta tacgacccaa tcaaaattta gagtgtatga aaactatgag gaaagctttg
     4801 aagattatgc caaattatta actaagggaa ttagtggaaa taaagatttt tatgcaggag
     4861 cattaaaagc taatagtaaa acatatcgag aagcaactaa attccttaca ggacggtatg
     4921 caacagatac acaatactat ttgaagctaa atgaattaat taaaacctac gatttaacca
     4981 actatgataa agaagttaaa ggtccacaaa tgagtaagac tggataccaa gtgcctattc
     5041 ataattattc aataaccagt ttgtttggaa atcgtggaaa tgaatttcac cgaggacttg
     5101 atatggcagc tacacagggt gagccgattc atgcagttaa agctggtaag gttgttaaag
     5161 cagagtttca ctactcttgg ggaaattacg tggtgataca acacgaagat ggatcaaccg
     5221 cgttatatgc tcaccagcaa caatacctag taaaaactgg agataatgtt ttgcaaggac
     5281 aaataattgg ctatgtagga agtactggga atagtactgg cccacacctt catttggaaa
     5341 tttgcaaaga tagtagtctt tctcagagca tgttagttga tccgaaaacc ttattattcg
     5401 gagactaaaa aaggtaatca atctgacaaa gtaacaagaa aatggttgga ttcttagacc
     5461 tttgatcagg atattgagac tggaattgat atctcgatta cgatgggttt gacaaataaa
     5521 acaagtccat tatcgaatat cgagtgtatt attgccgtct ttatgctcat attttgagca
     5581 ggtttcacta gatgaaaaca atttatctat cttgaggatt tcgtgtacgt atcgttcagt
     5641 cttgtattgt aaattagtca taaaatcaga ctaagaaaaa ttggaaatat ttttttctga
     5701 tttattaaaa gttaattgaa attatatggt acttctttct tgggagggta ggtgcagtga
     5761 aggaaagata tggtcacctt actgttgttg aggagattac tcataatgga tataaaaaat
     5821 atcgttgcct atgtgactgt gggaatgaaa cgattgtata tcgatcaaac ttactaacag
     5881 gtagaaccaa aagttgtgga tgtggaaatt ataaaaatag gcataaatat aaaaatttga
     5941 caaataaaac ttttggtaag ttagttgcaa tcaagccaac taaactgcga agtgaaggaa
     6001 ctgttgtttg ggaatgtcaa tgtctgtgtg ggagaaaaaa gttagtatcg gcaaagcgac
     6061 ttctaaaagg tgatgtaaaa agctgtggat gtttgaaatc aaaaaagcat cacattgaaa
     6121 ataaaaaatt cggattactt actgcaaagt atcctttgga caataactgt tcatggaaaa
     6181 caaaatgggt ttgtcattgt gagtgtggca gtatgtgtga gataagttat agtaacttat
     6241 tttttggaca tactcaaagt tgtggatgtt taaaaaggca agaaacaagg acatgtgttg
     6301 aagggacagt aatagagtgt ctaacgtcaa aattaccgaa aaataataca agtggtgtta
     6361 agggagttta tcgttctaaa gagaaataga ttgcgtatat aactttaaaa aaaaagaggt
     6421 actatttagg atcgtatgat tctattaaga gtgcagctca agctagaaaa gatgctgaaa
     6481 ataaattgtt tcaacctttg attgacaaat atcgaggtaa tcattaatta ttgtggataa
     6541 taattaacag tgatatataa acatctataa tatattattc atactattga tatttagtat
     6601 aatcctatgg aattgaatat catagcaatg tatttgaaaa gcaaattgga aaacaaatag
     6661 gtgcaacacc ataaatatga aaaaattggg aaaaagtaga atggaaattt gcttacgtct
     6721 cacgagatag ctcacactat taatctgaag aacaatgaag tcgactttta gaaattgcta
     6781 gaagaaagta aagcgaatta tcactagtta ctatagagta agttcaaata aagcatctcc
     6841 aacttacatg gttacatgag gatatcaaat cgaaattact caagcttcct atggctagag
     6901 tcgcgagaag ttgttgggca gtagacatag gttatattgt tcttagctac acgatcatta
     6961 gaaaggaaat cctattatgt atagttatca agaagatgca aaagtccaaa aaaatcgctg
     7021 gtggattttg attgctatat ctatgtttac cttcatgtcc acattggatg gcagtattgt
     7081 caatattgca ctacctacca tctcagatga tatgggcata ccaatgaatc aagcggaatg
     7141 ggtagtgtcc gtttatctga tgattgtctg ttcttgtttg ttgctgtttg gtaaaattgg
     7201 cgatagccgt gggaaaatca aagtatttcg gattggtacg ctgatctttg tcttgggatc
     7261 gctgttgtgt gggttcaatc attcgctagg tattttattg ttcgcacggg tggtacaagc
     7321 cattggtgct agtatgacta tggctacaaa tgccggaatc atcacggaag tctttcccat
     7381 acatgaacgt ggtcgggcat tgggagcaat aggtgcgttt gtttcattag gctcgattgc
     7441 tggaccaggt cttggcggct tgattttgtc ccaattttct tgggcgtaca ttttttggat
     7501 caatgtaccg gtgggaatcg taacgatgtt gattgctgaa aaattcttac caaaagatat
     7561 caccatgagc ggccatacaa tcgatggagg gggctttgct gccattgcgg ctacgattat
     7621 cagctttttt ggagcaatct ttttggggca agaaagtggc tttttccatc cagtccccct
     7681 tgtactgttc gtaatttcag tgattgcctt tgtcagcttt ttaaaagttg aaaagaaagc
     7741 agcctctcca ttgatttctt tcggtatttt taagaacaag atttttacca tgagcttgat
     7801 tacagccttg ttgatttttt cttccaactt ctttgtcaat gtagtgattc ctttttatct
     7861 gcaaaatgct cgcgggttga gtcctagcta tgctgggctg ttgatgatgg tctttccttt
     7921 gatgatggtc gtcggggcgc ctttaagcgg ctacctgacc gaccgggttg gcccggagat
     7981 tttggtatta gtaggcttgg gcttattgtc ggtaacccag gtcatgtata tgtttttgtc
     8041 agtggactct cctttgtggc tatacattgt cgctactgcc atcatgggtg ccggcaatgc
     8101 cttgttccag tcccccaata atacgatggt tatgagctcg gtcactatcg aaaacttggg
     8161 actggcaggc tcgatgaatt cctttgcccg aaatttgggg atggtcagcg ggatttcgct
     8221 ttctacgacg gtcttatatc aagcgatgtc tgctaagatg ggccagcaag taactaacta
     8281 tatcgcaaat cggccggatg tctttttata tggaatgaag attacctttt taggcagctt
     8341 tgttttgtgt tttgttgcct ttttcctgac tatctggcgt atcaaaaaac aaggaggtat
     8401 tcagaaaaat gtctgaaaaa accactgttg aagaattttt ggaggcctgc gtatgggtga
     8461 gtgggatttc catggatgat ggaagatgaa gtgaacttct aaaagccgaa aaactcaagt
     8521 attatcttca aaatactagt gctcaatgag atgcttgatt tgatcacaag aagactgcga
     8581 aagccgctgg caaccgcaag ctaggtatcc caccgatgga ctacgtttta gaggctactg
     8641 gttatgaata cggagccaac gctctcatct gcataagaat acatcaccca gattatgtaa
     8701 agattttcga tacttctgtc aaaaaatcat taagatagat aaatgatttc cgtctagtca
     8761 aattggttca gaatgtagac acacagatgg taagaagtca ctcacaattc gagaatggac
     8821 ttgtcctatt tgccgtatac atcataatcg aaacgtcaac gatagtatca atattttgac
     8881 caagggctat gaatacaagc attgccttag aaaacatcaa gaaccttagg aacaacgggg
     8941 atagcttggt taataagaga taccgctgtt taggcttgca agtatactct tttatcaaga
     9001 attccgtggc tttagtcatg agaggcttaa tactatgaaa attttcttta gcatttttga
     9061 tttaggttcc ttttctaatt ataataagat gaccactaca gtaacctact gaaatatata
     9121 aacagccact gtagtaccat tttcaaagta gtctatgaga ggagaatctt cattcttatg
     9181 aacgctggaa ttgcggatat gaatttaata aaaaaaaccc ttaatgattt tacttccaat
     9241 tcaatatcaa aaggtactgg tatcaattta agcacaataa aaaagctgaa aagtggtgaa
     9301 agatcagtgg aaaagctgaa tcttttagat gctataaaaa taacggaatt tgctatgaaa
     9361 aatggaaaag ctgagattga aatatggaga taattatcgt ctagtgaatt acttgccact
     9421 tagctgaatt taacgatcct tggtgcttga agggggagat tcttgggaat agagcatact
     9481 tgcaagccta tgtctttact aacaacgata tctcttatta accaagctat ccccgtagtt
     9541 ctaaggcttt tacttttatc taagcaataa tcgtattcaa aggaaatttt tgacccttag
     9601 aaagtctcat tttatcagac gtgagcttag gcattgctga attttgttaa ttcggtttta
     9661 ggttgtgctt tgagcataat atgaaagtat tctttatcac gatttcattc atctaaggta
     9721 atgtgatgtg attctgaaat tctttcaaag gcagtttttg cataagctga cattacatca
     9781 tcaatgacat gtttacaatg tttcactact aaaataaggg ggtaaccaag aagaaaaact
     9841 gaatgattat ttgtatctaa ttccatggta tatcaaccat ttcctatata gactgattat
     9901 gacatggatt aaaataaaaa ggcactatgt cttaattgtc ggttttcgag ttaccgaatg
     9961 gcattcatca cccacctata gaggatgggc gacttctgcc tgaaataagt tacactactt
    10021 ataacttcta ataaaattag attgagtcat tattatttaa catcaacgat tttccagttt
    10081 ccattttttt gtaatactaa tttgtactga aagtagctag tggtttttga tacattatca
    10141 aggtatttta tctttgtact cactagaaaa ttttttccgt tttgtagata aactggattc
    10201 tctaactccg aaaactttaa gtcaatattg attggtcgca tcgcaccatt ttcaacataa
    10261 tactccaatt ctttttcaga agctgatgga taaagtttaa aaaatgtttc taaaaaatcg
    10321 gtgatttctt ccgttgtttt ggcatcaatt tctgaatcgt tttctacttg ttttgcttca
    10381 tattttgcct tgtctggctt tccaatcatt gttggcaaac tagttactaa atgattcctg
    10441 ttttcatctt caaagattgt cactcgatat gcagaagaaa ttgattgcgt cttttttccc
    10501 tctgtaatat gttgctccac taaaaatgaa acgacaaatt cttccgagtg tttttcaaca
    10561 tctagaattt ttacagattg aacttcggaa ctcgtgggaa tatctgctcg aaccatatct
    10621 tggattaaag cgagaccgtt ttctgtaagg tactgttcga gattactcat tctcttttca
    10681 attgcttctt tgttattttt ccatgaaaaa tattctttcg caaaatcctc tgtaaaactt
    10741 tcaacgccag aagtattcac taccttctct tttataacca ctttttcatg agttgtatgt
    10801 tgatcaatgg cggtgaaatt tttataaacg ccaaaagcaa gactacaaga caataaaagc
    10861 caaagggcaa gcaccatttt tcgatgctta cccacggata caactcgttc ttttttcttt
    10921 tttgggctct tttccttttg attccgttca atttttatct tcattctttt tcctcttttc
    10981 tcttatttgt atcggccagc tccgataaga tgctgccgcc aatagttttc cgttagattc
    11041 gtccacccca ctggatctcc tgcatgaaac atgcgattct cccctgcgta aatgcccaca
    11101 tgggtcacat aagtaccggc atcataggtt gaatgaaaga aaactaaatc acctggtttg
    11161 gcatctttta gatcaatatg agtcatggca tcatattgat cctgagccac ccgaggaagt
    11221 ttcaggtcaa ctttggcata acaccattgg gtcaagccac tacaatcaaa ccccgtttct
    11281 ggacttgagc caccaaacac ataggccgtt ccttcatact taaatgcctc atcaaaaatt
    11341 tcttggacta ttttgtcatc aaactgagtt gtagttaggt attgcttcac taataaacag
    11401 taaaacatat tgccataatt ataccgccac ccaccatttt ccttaatcgc gattgggttg
    11461 gggtaatcga ctttttgacc acctgatttt tcctttgaaa aagattcagc taaagcaaaa
    11521 ctatgcttct tcccgttttt cccgacatga tctagatacc cgccaccata gttataggcc
    11581 tgaataatac tatcttgatc acaccctagt tctttggaac gcttaaccaa agacgcaaaa
    11641 tagagacagc cttgtttgat ggatgcctcg gtacttaatg aattaggtgg taatccggct
    11701 gactcagaac tttgcataac gtcttccatt gtgccaccag attccacttg aataatcgcc
    11761 aaaagaattg gaatatactc tgttatatcc gattctttgg catacttttc caccattgca
    11821 cggtgtttca atacttcttc tgagacacta gttccgccag gagaagtgcc agaactacca
    11881 tttccctcac tgtcttcacc aaacaacaaa cccacacaca tcaaaatacc cataaaagag
    11941 atggtcagaa aagttaatga aataacaccc actttcttca agttcatttt ttctcacctg
    12001 accttgattt tggacgatgg ttcattcttt tttgtacttt ttgattggtc tgctttggca
    12061 gagtggacct ctgtttcact tgttggaaag aatttttctt acttgctgat tggcttttcc
    12121 cttttgaaat gatttcttga ctagttagtt ttcgtttgat ttcgggattc agatttgctc
    12181 tcacaatccg ctttggtgct tcttttttca gcatcatatc tttctgaggt tgagctgctt
    12241 tatctattgg aatattcgcc cttctttttc cctgacgatt tgttgcttca ctcagtatct
    12301 tgcgtttttc gtccattttc gcatttcttc gttgtcgttg tttttctcgc aaatccactc
    12361 gatccgcttt ttcttggaac agtccgcgct tgaattcttc tggtgctttt ttcgactttt
    12421 cgattccttt atggagattg tatttcaaat tcgttggcgt attccttacc tgatccttta
    12481 cttgtttagt cttatctttc accaaagctt gggtatccaa aactttgccg atttttctcc
    12541 cagccaaatt cattcgggat tgtttcttat tatttgaaac tggattctcc ttagttaagt
    12601 cagatagtat ttcgtgatcg ttcggtaatc gtgaattctt tcgtagagaa gaaccggatg
    12661 gcttaaattt ggattttgat tgttcctttg ccaacttatt tccgactgct gcaccaccac
    12721 caaaagccaa agctcgtccc acatttcgat tgagttgccg caattttcga ttcattaaca
    12781 tttgcggttt tcgcatgacg cgtcttccca ttgactggct atcattactc tggaggttga
    12841 acatactcat aatgtcgccc aatttgaaat agataccggc aaaggtcaca atttgcagaa
    12901 aagcaaccat gaaaaatgga taggttgccg aaatattgaa aaacatcgtg gagatgctaa
    12961 atgcagtcgt aatcaccaaa gtaacacccg ctcgcatcat aatcgtattg aataaacgaa
    13021 taatcgcctt ctttcctaaa ctttcataag tcggcaacat ggacaataag aaactaattg
    13081 gcagaaacat cgcaaagata atgaagagaa tttgcgagaa caacataatc cctgttagaa
    13141 gaaaaacaaa gaaggaaatg ccaatattga acaataccaa aaagaccacg gtccctaaac
    13201 ggttcatggt cttggtaata ctgagattcg cattgtcatt gtcttcaatc tctgccttga
    13261 ccgcttcttc ccgatctttg cctttgtttt catctggact cacagataga atcttgttga
    13321 cccggtcttc accaatttct tctatgttgg aatcatcaaa ttgcagtagt aaccaaggtt
    13381 gttggacttg aatcgaaaac aaactatccc gaatcaaatc cacactatct tttccttggc
    13441 tttctgaatt gggtacaacg atctcggttc caagggtaag ggccgcttca ctaacatctg
    13501 agctaaagtc attgattttt gtgatatagg taggcgcata agcaatgaac gaacccgata
    13561 gtagaaaaat catcacaaaa ttaatcacgg ctcgaactgc tttcgtggtt tctcgtttta
    13621 ataatccggt atacgccaca tacaccccca gagctaaaat catcaataat agaaacccaa
    13681 aataaaatcc ggaggtctgg agtccatttt cagtaatacc agccaatgtt tggatattct
    13741 ttccaatact ttcagccgta tccgaaatga aatctaattt gtaagcctct tgaaccacat
    13801 aacccgtggc gttcgacaaa tacaaactca ccgtccaaat gaaattagta atggcataaa
    13861 gaccatacat cacgcttttc cctaagccat caccccaatt ccaaggcaac caatcccaag
    13921 agctatccac aaagaaatct agttgatagt tgttcagtga atatttggaa tatagatttc
    13981 ctgcttcaac tgtatcatca actagccctg tcgcctcggc taccgtacca actaaactca
    14041 atagaactat cagagtgagt atgctaataa aaacgattcc cacaatccga aaaagtttct
    14101 ttttcatccc ctcactccat tctctcttga acaggtggac gggtatcaaa ggcatggaag
    14161 agttcttcaa agactggatg tacctgaacc acgcctaccc gaccatacaa atcctgcatc
    14221 aagcattggc cattttccaa atctcgtagg cgtttttgat tgctttcatc ttctcgatcc
    14281 aagccaaaga attccaaggt tttcttgatt tcatgaatat cggtactgcg aaacgcaaac
    14341 ttcaacccga tattattctt caagcgttca tccagtaaat catcactatt ttgcgtgaca
    14401 aagtaaaccc cggcattcat agcccgccca gcacgaacga gcttattcga taatgttttc
    14461 ccttgcgcta cttgaagaaa actccacgcc tcatctaagt ctacaatttt gaagatttct
    14521 cgattactat ggataaagtc caaggcaaaa gtggaaatga cgattagcat ggaaacacta
    14581 agcaattcca tggtggtata ttcttcaaaa gtggtttctg catctggtaa aaccaaatcc
    14641 gctacttgaa tgatatttaa ctgcctatct aaactaatcg actgggtaat cgaaccatct
    14701 gagaaaagca aatgggcaaa atcataatcg gtaaaggact cgatgtgatc cgcgatattt
    14761 tccgcaactg gtgagccttc ttttcgaagc tcattaatta cttgtaataa tccccgttga
    14821 ttgctttggg taaccgtccg aatggcctta cgcaacactg gaaacttctc cccatctcga
    14881 ctagaaattc ctgttaaaaa ggtcagaata tcaattgcta gactttcaga atccttcttt
    14941 ttctccataa tcacataagg atcaagtaat ccttgatttt ttggcttact agttaagttg
    15001 agaatattga tttcatccgc aatttctggc aaggtttctt tccagccacc ccgttatagc
    15061 gataggtaac tttttgatgt tatctccaag ttttcccccg cttcacaccg tacatgaaac
    15121 tttcgcttca tacggcgttc catcatatcc aaatagattt ataagttatt tcccagttat
    15181 tgtctggtat ccgtaattcg tccgtattct gttcagtttt ttcttctgct gactattaag
    15241 aacataacct tttaacagtg attttgcttc gtctgctgat tctgttagga ttgcctttgc
    15301 aatgggaatt tccacaaatg ccagattaga atatctgtca aggttatctc ctttgtctga
    15361 tttgtgaata catatcagag tggacggagt tcctcgacga ttcattacat agcagttgcc
    15421 ttgttgtgcg atatatgccg aaatacgatt gtcgttaaac tctacgctat cggcagggtt
    15481 ttcattttcc aacaacgctc ggatttcttc ttttgttaca actacaacgt cctcatgcac
    15541 cttgtttctc ccttgctttg taaaattaca aatatcttgc gagaaattca taggattttt
    15601 gtgttgtact ccttgaatcg gcaatagcgg attatccgca atcatcacta tttttgtttt
    15661 tggtctgatt ccttttgtcc tgctctgaaa ttctgaactt gtactttcaa atggaactga
    15721 ttttgcaatg ttccttaaac ggattcgtat ggtcggtaag agagcgtaac ttacttctgt
    15781 caggttgtta tatacgttag ttgctatgca gtagtaattc tgcataccga taactgtcag
    15841 attatatctg ctgatattga agccagtcgt atgtctggct atatctttta cctttaactt
    15901 tagatttgtt ttcgctttct ttaatgcttt ttggttcata tctgttttgg caacataacc
    15961 gtgtttcgtt cttccttttg gaatgacttt gattttgaac cccagaaaat ctgatgaatt
    16021 tttcttcaga tttactacct ttgatttctc tggactgatt tctaatttca gccttttatt
    16081 aagaaaatct acggtactgt ggtaaaatct ttgtgcttct ccataggttg aacacatgat
    16141 tttaaaatca tcagcgtaac gtacaataaa accgcttttc agtttggtat atttctttgc
    16201 gtattgaagc cacccatttt tactgcggtt cttcggctgg aatgtttccc actgactgct
    16261 tacccaccag tctaattcat taaggactat gagggagagc agtggactta acagaccgcc
    16321 ttgtggcgtt cctttttctg ggacaccctc gccgtcaatt tcggctttca gcattttgct
    16381 gataatgcaa atcaatcttt tatcccgaat accaagtgtc cagatttgtt ttaatagttt
    16441 tccatgattg acattatcga aaaatccttt aatgtctacg tcaacacagt aatgatattt
    16501 tgataaattt atcagtgtcg ttactctgct gactgcatgg tgggcacttc gattcgcacg
    16561 gaatccatag gaatgattat ggaactttgg ttcgcatatc ggctctagga cttgcaggat
    16621 acattgttgt accagcctgt cccagataca cggaatcccc aatggacgca ttttatcgct
    16681 cccagctttt ggaatgaaaa cacgtctgac tgatttgggg cggtagttgc taaggctttc
    16741 ccgaacggta gcaacaatct cagcattgct gagttgtttt atatcgtcta tcgtcattcc
    16801 atccgttcca gccgttttac tgccactgtt tgatttcaga ttacggtaag ctaaacaaat
    16861 attttgttcc gaaccaataa tttcaagtaa cttataaaag ttattaccgt tcttgctctg
    16921 tgcgtataac tcgtcataaa tatgttgcat atcataatac tcgttatagc ggagcttgtt
    16981 tttctttagc atggtctgta ccctccttgt ttgcaggagc aagtacatct cttagtctta
    17041 ctcgaaccca taccattttt cttagtaatt ctattcttca tatgactagt ggctatccct
    17101 ccaccgcttg ttatcacggt ttcataggta ctgtgccacc actctcactg acattaacac
    17161 accttatatc attgctaatc tcacaataac tttctgatgt gaacacttca cagacagtaa
    17221 aatctccatg ttgtcagctt tcaacgttcc aaacacacta tctgtacata tatccttagg
    17281 tttcctctat aagcctgcta ccttgataac gcctttaacg ttatatggat tttcataaat
    17341 gcggagtctt actcacccac agtagcgaca ctgtttgtgt cactggacat ttctatccag
    17401 ccccgatata gacctgtaca ttcaaggatt cgtcagtaga cctctcatct acattctaac
    17461 catagatatt ttatagactt ccggcatata ggacacaacg cccacctctg ggaatctttc
    17521 gtcagcatta tctgttacgg tgtctgtctg ccgacttcac cgagcttctg acagagcctt
    17581 gtttactcaa gtttctgcca gtcagagtat taaagtagcc tttcagggcg ttaccccgtc
    17641 attcgactat tcgtgtgttt agttctccaa cacacattaa atattgattt aatgaagtgc
    17701 tgtgcctact cttttcaagt aggaacgttt cgcaccccct tttggatcga caatcacagc
    17761 ttgtccacca aaaaggacgg cgtaataaac taagagattg ttactaaagg actttccgcc
    17821 accgagtgaa ccgaggaatg ccgctgctag cgcattggta actgaatcct tgaccccttg
    17881 tgaggccaat gccggtttca ggtaaacatt gcgccccgta tctacgttat aaccaaagta
    17941 aatgccttcc aactccccta acatctgagt ggcgccaaaa ccaagtccgg cgagaaaatc
    18001 agacgtaaca tactggatat aatcattcat gtatcgctta ctagaaggta agaattcttc
    18061 atgtagcccc atcatatcgc caaaaggccg gactagtttt acattggtat catcataaaa
    18121 atctagcact tcatcacacc ggcgctttag ttcatctact gattcggcac ttactcgaac
    18181 cacatagctc agtttgtaca tggattcttt ggactgatcc aaggtagttt ccaattcatc
    18241 tacggaatcc aatgcatcta agacattgga atttgtttca ttgtcagatt gataggcatg
    18301 gttgtccaag tcttttaatt cctttttctt attgcgaacg gtagcaagtg cctttttgtt
    18361 cgtcacaatc tccacattca tactagtgtc aattgggaat gtaaattgtt gttgctggta
    18421 atagaaaagt tctgaggagg gaaactccat ttcccccaca attgtattaa ttgttaagta
    18481 cgctacatat gattcatgat tctcatgttc catgcataag taccggggct tttcttcaat
    18541 caaacaacga ctgggacgta ataagtcgta acgtttcacc aaagtttccg acttcagctt
    18601 tttctttggt agctgaaatt catattcttc aaatggtgta cccttttcgc catagatatg
    18661 ttcaatcaaa tacatcagat cactcggtgt gactcgtctg actttaaatc ttcgagccaa
    18721 tttactttcc attagttttt caagcttggt atatcggcga atttcttcat tggataagga
    18781 aacaaaatcg cccatcaaat gatgattcac tccatagaca aattcttgaa agccggagaa
    18841 gaaagatttc ttgagattct ttagattgac ctcttcatct gtcgcaatca gtttgaagcc
    18901 aataaaaaag cgataatcaa tctgactatc gccaatcatg gatactaatg cctctgtctg
    18961 taaatcaatt cgttgtttcg ctacttctgt taatcgcccg gtaatttcct tttttgatcg
    19021 ttcttgcgtg gcacgaatac tactttctgt ggcaatttgt aaggcatgaa tcttgccttc
    19081 acgattttgc gcaatcaatt gacgaaagtt atcgtggact tgatatttct gttcaggtga
    19141 caaaaaggag taattgtaag gtattaactc atagtaggca aaacattctc cctcttgatt
    19201 aaagacaaga ttattttcaa tatacttaat cgggtacata gttcacactc cttaccagag
    19261 taatgggttc gtcccatcgt ttcttctgtt ctttgacttt ctttcctgcg aaagtgacct
    19321 taaatcgtaa gtgatagcta ataaaagatc gtaaaaatcc caatggtttc ttaccgtcaa
    19381 acgttttttg cgacatgaac caagtaactg ccaccggtat acctaagtat tttaaaaagg
    19441 ccccctctat catagatagt ggaggcacat gggcaaatac gatgacaaac agcaaggata
    19501 aaacaaacca agccatctgg ttaaaagtca caggaaaagg cagttgaaag tcattaattg
    19561 cataaatgac cttttcaact gcccaaatac tggtgtagct ttttattttc ttcatcaaat
    19621 cattccttta aaaagaagga tagtttagaa atcaactacc cttcaataat tttgttaata
    19681 tggatactca aaaacacctc gactcgttac caaataattg cctccaatct ccaagtctct
    19741 accacacgca tcaaaatcaa tatacgtctt caagtgttct ggtacttctc caaaatcacc
    19801 agcttctaca atcaagaaat atgctacatc actcatatca tcgcaatctg gataatagat
    19861 aatatcatcc acatgctcta ccatctcctc aaaagaattg aagaaagcat gttgaatctc
    19921 agaagcaact tcaccaactg gcgttccttc aagttcctcc gctaagccac acaaccgatt
    19981 aatttcctca attggcgtat actcatcaat ctcaaaggga agttcataat catgaattgc
    20041 atattcctcg tattcgtcat ttaacccaat tcgttctttc acttcatcaa aatcgacagg
    20101 tggggtaaac cacgccccca ccaattcacc ttcgttatat ttcccgaggt tcgcaatata
    20161 aacacgcatc tgttccatcg tgcttccccc ttacgcaccg acaattcggt taaacaaact
    20221 gagtaagaca tccttcaccc cagaagcatt aaagaccaat ccgaccgcaa tcaaagcaat
    20281 caccaaaaag ccaatcagtt tggaaaactc ccgcttaaat cctaaaaaca aaccaatgac
    20341 cacaattgcc agtaagacta aggattg
//
LOCUS       DAYLZO010000048        20002 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.049, whole genome shotgun sequence.
ACCESSION   DAYLZO010000048 DAYLZO010000000
VERSION     DAYLZO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.049"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..646)
                     /locus_tag="ACGVJG_001563"
     CDS             complement(<1..646)
                     /locus_tag="ACGVJG_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFJ6557507.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFL"
     gene            895..1416
                     /locus_tag="ACGVJG_001564"
     CDS             895..1416
                     /locus_tag="ACGVJG_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFJ6557508.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(1602..3332)
                     /gene="spxB"
                     /locus_tag="ACGVJG_001565"
     CDS             complement(1602..3332)
                     /gene="spxB"
                     /locus_tag="ACGVJG_001565"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFJ6557509.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            3497..4315
                     /locus_tag="ACGVJG_001566"
     CDS             3497..4315
                     /locus_tag="ACGVJG_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFJ6557510.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(4418..4615)
                     /locus_tag="ACGVJG_001567"
     CDS             complement(4418..4615)
                     /locus_tag="ACGVJG_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557511.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            4784..5362
                     /locus_tag="ACGVJG_001568"
     CDS             4784..5362
                     /locus_tag="ACGVJG_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFJ6557512.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            5457..5594
                     /locus_tag="ACGVJG_001569"
     CDS             5457..5594
                     /locus_tag="ACGVJG_001569"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557513.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(6407..6910)
                     /locus_tag="ACGVJG_001570"
     CDS             complement(6407..6910)
                     /locus_tag="ACGVJG_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFJ6557514.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            7269..8294
                     /locus_tag="ACGVJG_001571"
     CDS             7269..8294
                     /locus_tag="ACGVJG_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFJ6557515.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            8617..9495
                     /locus_tag="ACGVJG_001572"
     CDS             8617..9495
                     /locus_tag="ACGVJG_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFJ6557516.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            9971..10087
                     /locus_tag="ACGVJG_001573"
     CDS             9971..10087
                     /locus_tag="ACGVJG_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFJ6557517.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            11015..11353
                     /locus_tag="ACGVJG_001574"
     CDS             11015..11353
                     /locus_tag="ACGVJG_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557518.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            11447..11677
                     /locus_tag="ACGVJG_001575"
     CDS             11447..11677
                     /locus_tag="ACGVJG_001575"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFJ6557519.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            12077..12448
                     /locus_tag="ACGVJG_001576"
     CDS             12077..12448
                     /locus_tag="ACGVJG_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFJ6557520.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(12722..13414)
                     /locus_tag="ACGVJG_001577"
     CDS             complement(12722..13414)
                     /locus_tag="ACGVJG_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFJ6557521.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(13417..14202)
                     /locus_tag="ACGVJG_001578"
     CDS             complement(13417..14202)
                     /locus_tag="ACGVJG_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFJ6557522.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            14439..15839
                     /gene="lacG"
                     /locus_tag="ACGVJG_001579"
     CDS             14439..15839
                     /gene="lacG"
                     /locus_tag="ACGVJG_001579"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFJ6557523.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            15864..16181
                     /locus_tag="ACGVJG_001580"
     CDS             15864..16181
                     /locus_tag="ACGVJG_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFJ6557524.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            16194..17867
                     /locus_tag="ACGVJG_001581"
     CDS             16194..17867
                     /locus_tag="ACGVJG_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFJ6557525.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            17881..19512
                     /locus_tag="ACGVJG_001582"
     CDS             17881..19512
                     /locus_tag="ACGVJG_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFJ6557526.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT         6236 a         3333 c         4052 g         6381 t
ORIGIN      
        1 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
       61 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
      121 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
      181 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
      241 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
      301 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
      361 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
      421 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
      481 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
      541 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
      601 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
      661 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
      721 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
      781 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
      841 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
      901 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
      961 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
     1021 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
     1081 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
     1141 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
     1201 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
     1261 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
     1321 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
     1381 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
     1441 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
     1501 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
     1561 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
     1621 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
     1681 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
     1741 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
     1801 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
     1861 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
     1921 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
     1981 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
     2041 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
     2101 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
     2161 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
     2221 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
     2281 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
     2341 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
     2401 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
     2461 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
     2521 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
     2581 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
     2641 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
     2701 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
     2761 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
     2821 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
     2881 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
     2941 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
     3001 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
     3061 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
     3121 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
     3181 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
     3241 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
     3301 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
     3361 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
     3421 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
     3481 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
     3541 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
     3601 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
     3661 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
     3721 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
     3781 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
     3841 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
     3901 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
     3961 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
     4021 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
     4081 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
     4141 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
     4201 ggaagaattg atccctgaag cacagcagac attgtccagc aaacatcatt ttgcagtttt
     4261 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
     4321 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
     4381 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
     4441 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
     4501 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
     4561 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
     4621 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
     4681 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
     4741 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
     4801 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
     4861 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
     4921 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
     4981 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
     5041 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
     5101 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
     5161 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
     5221 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
     5281 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
     5341 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
     5401 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
     5461 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
     5521 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
     5581 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
     5641 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
     5701 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
     5761 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
     5821 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
     5881 aaaataacat atctcatttt acacttttgt tattttatac ttaaaaaata acgacacaga
     5941 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
     6001 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
     6061 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
     6121 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
     6181 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
     6241 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
     6301 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
     6361 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
     6421 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
     6481 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
     6541 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
     6601 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
     6661 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
     6721 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
     6781 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
     6841 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
     6901 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
     6961 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
     7021 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
     7081 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
     7141 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
     7201 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
     7261 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
     7321 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
     7381 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
     7441 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
     7501 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
     7561 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
     7621 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
     7681 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
     7741 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
     7801 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
     7861 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
     7921 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
     7981 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
     8041 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
     8101 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
     8161 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
     8221 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
     8281 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
     8341 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
     8401 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt
     8461 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
     8521 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
     8581 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
     8641 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
     8701 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
     8761 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
     8821 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
     8881 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
     8941 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
     9001 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
     9061 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
     9121 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
     9181 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
     9241 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
     9301 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
     9361 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
     9421 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
     9481 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
     9541 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
     9601 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
     9661 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
     9721 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
     9781 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
     9841 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
     9901 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
     9961 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
    10021 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
    10081 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
    10141 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
    10201 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
    10261 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
    10321 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
    10381 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
    10441 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
    10501 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
    10561 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
    10621 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
    10681 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
    10741 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
    10801 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
    10861 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
    10921 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
    10981 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
    11041 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
    11101 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
    11161 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
    11221 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
    11281 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
    11341 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
    11401 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
    11461 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
    11521 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
    11581 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
    11641 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
    11701 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa
    11761 tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct
    11821 acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta
    11881 gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa
    11941 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
    12001 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
    12061 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
    12121 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
    12181 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
    12241 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
    12301 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
    12361 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
    12421 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
    12481 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
    12541 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
    12601 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
    12661 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
    12721 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
    12781 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
    12841 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
    12901 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
    12961 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
    13021 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
    13081 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
    13141 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
    13201 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
    13261 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
    13321 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
    13381 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
    13441 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
    13501 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
    13561 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
    13621 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
    13681 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
    13741 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
    13801 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
    13861 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
    13921 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
    13981 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
    14041 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
    14101 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
    14161 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
    14221 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
    14281 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
    14341 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
    14401 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
    14461 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
    14521 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
    14581 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
    14641 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
    14701 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
    14761 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
    14821 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
    14881 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
    14941 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
    15001 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
    15061 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
    15121 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
    15181 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
    15241 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
    15301 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
    15361 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
    15421 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
    15481 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
    15541 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
    15601 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
    15661 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
    15721 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
    15781 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
    15841 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
    15901 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
    15961 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
    16021 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
    16081 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
    16141 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
    16201 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
    16261 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
    16321 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
    16381 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
    16441 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
    16501 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
    16561 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
    16621 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
    16681 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
    16741 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
    16801 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
    16861 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
    16921 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
    16981 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
    17041 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
    17101 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
    17161 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
    17221 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
    17281 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
    17341 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
    17401 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
    17461 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
    17521 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
    17581 ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
    17641 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
    17701 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
    17761 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
    17821 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
    17881 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
    17941 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
    18001 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
    18061 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
    18121 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
    18181 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
    18241 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
    18301 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
    18361 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
    18421 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
    18481 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
    18541 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
    18601 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
    18661 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
    18721 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
    18781 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
    18841 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
    18901 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
    18961 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
    19021 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
    19081 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
    19141 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
    19201 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
    19261 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
    19321 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
    19381 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
    19441 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
    19501 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
    19561 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
    19621 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
    19681 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
    19741 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
    19801 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
    19861 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
    19921 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
    19981 ccctccatat tatgttggct ct
//
LOCUS       DAYLZO010000049        19980 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.050, whole genome shotgun sequence.
ACCESSION   DAYLZO010000049 DAYLZO010000000
VERSION     DAYLZO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.050"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..182)
                     /locus_tag="ACGVJG_001583"
     CDS             complement(<1..182)
                     /locus_tag="ACGVJG_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFJ6557527.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGI"
     gene            609..1388
                     /locus_tag="ACGVJG_001584"
     CDS             609..1388
                     /locus_tag="ACGVJG_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557528.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1434..2378
                     /locus_tag="ACGVJG_001585"
     CDS             1434..2378
                     /locus_tag="ACGVJG_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFJ6557529.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2394..3173
                     /locus_tag="ACGVJG_001586"
     CDS             2394..3173
                     /locus_tag="ACGVJG_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFJ6557530.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            3185..3883
                     /locus_tag="ACGVJG_001587"
     CDS             3185..3883
                     /locus_tag="ACGVJG_001587"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFJ6557531.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3911..4675
                     /locus_tag="ACGVJG_001588"
     CDS             3911..4675
                     /locus_tag="ACGVJG_001588"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFJ6557532.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4719..6170
                     /locus_tag="ACGVJG_001589"
     CDS             4719..6170
                     /locus_tag="ACGVJG_001589"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFJ6557533.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            6202..7338
                     /locus_tag="ACGVJG_001590"
     CDS             6202..7338
                     /locus_tag="ACGVJG_001590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFJ6557534.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7368..8444
                     /locus_tag="ACGVJG_001591"
     CDS             7368..8444
                     /locus_tag="ACGVJG_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFJ6557535.1"
                     /translation="MHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKSDYDEEIISLG
                     GKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKAAKKAGIPIRI
                     AHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKFTLIPNAVDAK
                     KFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFHAIKNNSVLLI
                     IGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEA
                     QFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKAD
                     YNIQTAYKILESKYYELFELIQRG"
     gene            8450..9472
                     /locus_tag="ACGVJG_001592"
     CDS             8450..9472
                     /locus_tag="ACGVJG_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFJ6557536.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9494..10549
                     /locus_tag="ACGVJG_001593"
     CDS             9494..10549
                     /locus_tag="ACGVJG_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFJ6557537.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            11167..12402
                     /locus_tag="ACGVJG_001594"
     CDS             11167..12402
                     /locus_tag="ACGVJG_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557538.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            12486..13574
                     /locus_tag="ACGVJG_001595"
     CDS             12486..13574
                     /locus_tag="ACGVJG_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFJ6557539.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            13571..14992
                     /locus_tag="ACGVJG_001596"
     CDS             13571..14992
                     /locus_tag="ACGVJG_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFJ6557540.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            15028..15744
                     /locus_tag="ACGVJG_001597"
     CDS             15028..15744
                     /locus_tag="ACGVJG_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFJ6557541.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            15763..17346
                     /locus_tag="ACGVJG_001598"
     CDS             15763..17346
                     /locus_tag="ACGVJG_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFJ6557542.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            17475..18059
                     /locus_tag="ACGVJG_001599"
                     /pseudo
     CDS             17475..18059
                     /locus_tag="ACGVJG_001599"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            18005..18523
                     /locus_tag="ACGVJG_001600"
     CDS             18005..18523
                     /locus_tag="ACGVJG_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557543.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            18531..19571
                     /locus_tag="ACGVJG_001601"
     CDS             18531..19571
                     /locus_tag="ACGVJG_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFJ6557544.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            19820..>19980
                     /locus_tag="ACGVJG_001602"
     CDS             19820..>19980
                     /locus_tag="ACGVJG_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFJ6557545.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         7267 a         2533 c         3889 g         6291 t
ORIGIN      
        1 attatgcccc atataacaca agcaataatc actgtatcgc gttgcttcag ttgatcagta
       61 tttcttcgat ttttaatctt atctggtaca caattgtggt atatgttaga aacagttctc
      121 ataatttcct tgaatgttat aaaagtatct gtataatgtt cagtatattt tgatgaacgc
      181 atatgtagtc ctcatttctt gggagttttg agactactat atgcgttttt ttgttgattt
      241 accaacatgt ttagacattt tcctctagga aaaaattaac tagcaccacg ggtataacta
      301 ttattaattc aatgttaatt tttttgttat tctatttttt gtgtaacttt taaaaacacc
      361 tatgaaaatt atggaaataa aaatgaaatc gattaatttt tatcttatta atgttttatt
      421 tacttttaaa tcgtaaattg ccctagtcaa gaaaacagtt tgtggtatga ttatataggg
      481 aaaacttgtt tacaaatatt tctgtgaata tattagttta attatgtttt tttaaataaa
      541 aagctgtcat ttttatgtaa gtgaattata tcttaattta tctatcatca atacttagga
      601 gtgaatctat ggaaaagcca ttcgtcaagt cattgactgc tgtggcggta ggtgcaggtg
      661 ctattgcgat ctgtttgttt ggataccata tcaataatca gcggcaacat cagcaacgga
      721 tcaattatgc agaatctgcc atcaccaatc aaaaagacac cttgacttct ttatcaaaag
      781 aagtagacaa gctgtatagt acaaaagaaa aaatttttct aaaccctgaa ataacagaag
      841 aaacaatcac aagtttgtca aataagctat cctctgtcaa actttcagca gatgattacg
      901 acatcaaaga atcagaattg cctaaagaag ctgttgcaat ccaagatgag aagaaagcaa
      961 tcgtgacaca gttagatagt gcagaatcaa aactgaagat ccagacagct gtcaacgatt
     1021 tatttactaa aaatgtatca aactggcagc aagctgtaga cgatgtcatc atcaaagaaa
     1081 aggtgacaag tgcagatgtc gctcgtgtac gtgaaaatat gagctttttt aaagatagtc
     1141 cttggaaaac ggttgttatg caatatctag gattcgctga tattcagatc gcgcaagtca
     1201 cgcaactaga tcagttattc gatacgatgt taaaggatgg tcaagtgact gccaccgcta
     1261 cttacgatca atatttgacg gtgctcagtc aaatagagca gatccggaat gaaaaaataa
     1321 gcgcagcata cgcaacaaaa gcacaaactg ttgctgagca aatgggctat agcaatataa
     1381 gctattgaca gttaaaacgt tgattcaagc aaggttagca aacggaggaa actatggcaa
     1441 gacgaagaag aaaatcaaga gggaaaaaaa tatttctgat tattttagca gttcttatcg
     1501 tattagtatt agcaattgta ggagtaggag caaaattata tctggatctc tcacattcta
     1561 ttgagaaaac ttacgaatca gtggaacgaa cccaaggaga acgaacgaaa ccagttgatc
     1621 tggacaaaca agaatctttc tctgttctat tgatggggat tgatactgga gatttaggtc
     1681 gggtcgatca aggaagatca gatacgatga tggttgccac agtcagtcct gaagacaaac
     1741 agacaacgat cgtcagcatt cctcgtgata cgtatgtcga catcgtaggt cgtgacacaa
     1801 aagacaagat caaccacgct tacgcattcg gcggagctgc catggcgatg gataccgtac
     1861 aaaaatatgt agacatccca atcgatcact atgtctctat caatatgaaa gggctaaagg
     1921 aactcgttga tgcagttggt ggtatcgaag tcaataatga cctcactttc tcacaagatg
     1981 gctatgactt tccaatcggt aaaattacat tgaacgggga tcaggcatta tcttattctc
     2041 gtatgcgtta tgaagaccca aatggtgatt acggtagaca agggcgccaa agaaagatcg
     2101 tagaaggcgt agcgaaaaaa gtattgagtc taaacggtgt cagcaactat caatccgttt
     2161 tgaatgctct agagtcgaat atgaaaacag acatgaactt tgatgaaatg aaaaaaattg
     2221 ctttagatta tcgggaggca tttggtacga ttaagcaaga tcagctgcaa ggagaaggct
     2281 tcatgcaaga taatatctcc tatcaaaaag tcagtgatca agaacttgat cgtgtgaaaa
     2341 aagaattaaa agatcaaatg aatctagaga ataaatagga aagtggagaa aaaatggaag
     2401 agacaatcag cttgaaagaa atttttgaaa cattacgcaa acacttggta ataatcatca
     2461 tctcaatgtt tgctggcttg gccatctcag gaatcgtaac atttttcatc ttgacaccaa
     2521 aatacagttc acaagcacaa ttgatcgtga cactgccaca atcagacact gcaaatgtca
     2581 atgatgtcaa tacgaacctg caaatgatca atacgtataa agatatgatc gtcagtgatt
     2641 tagtgctcaa tgaagtcaaa gatcgtttag aaacagaaga caatgtgaaa atgactgctg
     2701 aacaaatcaa agatgccatc tcagttaatc aggcagaaaa ttcacaaatg ttctcgattc
     2761 aggcaataag tacgaatgca gtggctgctc aacagatcgc taacacaact tcacaagttt
     2821 tccaagagaa cgcaaaagat gtgatgaatg ttgataaaat ctcgatcatt tcaggtgcag
     2881 ttgccaatac gacacctgtt tcgccaaata ataaattgaa tttaatgact ggcttaatgt
     2941 taggaatgat ggtaggtgta ggtctggcat tcttattgga attactcgat cggacagtga
     3001 aagatgaaaa atatgtgaca gatacattag gattcccaat cttaggaaca gttccagaaa
     3061 tgtcagcgaa agaattgaat gctggtatcc gtaaaaaagg accagctcag acacaaccaa
     3121 aagctacaaa cgataaaaca gcgccatcac gcagaagccg ctcaagagtc taggaggaaa
     3181 gaaaatggca cgaacacaga aacaaaaagc agcccaaact catgcagtca gtttggtcac
     3241 actcaccaat ccctcttcac caatcgctga acaatatcgt acgattcgga cgaatatcca
     3301 gtttgcctcc gcagctggtc agcaaatcaa aacgattgtt gtgacttcat caggacctgg
     3361 agaagggaaa tcgaccactg cagcaaacat tgccgttgtt tttgccaaat ctggtcaacg
     3421 ggtgctgcta gtcgatgcag acttacgtaa gcccgtcatc tataaaacgt tcaagttgaa
     3481 caacgcaagc ggattaagta ctgttcttag ttcatcagga agtgtagcag atgagatcca
     3541 acgaacacca gtcgacaatt tgtcgattct gccaagtgga cctaaaccac caaatccatc
     3601 agaactacta agttcgccaa gaatggatca gattttagca gaagcaagac aactatttga
     3661 tgtagtgatc ttcgacatgc cgccagtcgt tgccgtaaca gatgcacaga tcatgtcttc
     3721 aaaaacggat ggcaccttat tggtcgtacg ggaaaatacg tcacgaaaag aatcattgac
     3781 taaagcaaaa gaactgttag atatggtaca agcacgagtc ttaggtgtcg tatacaacgg
     3841 agcagaacac agcaaagatg ctggttatta ctacggaaat taaggaataa gaagagagga
     3901 gacaagagaa atgatcgatc tacattgcca tatcctacca ggaatcgatg acggggcaga
     3961 aaacttagaa gcaagtattg caatggctga aaaagcgatt cagcaaggca ttacgcatat
     4021 tttatgcaca ccacaccaca ataatggaaa atacagcaat gaaaaatcac aagtcatttc
     4081 tctagtcgct tcgttacaag ccgaattaga gaaaagacag ttgcctttga cgttattgga
     4141 aggacaagaa gtacgtatta ccggaacgct catcgaagac atccaccgag atgaaatttt
     4201 atttacagat ttagatgata catatcttct gatcgaattt ccgacactgg aagtgccgtt
     4261 atatgcagaa cggctttttt ttgagttgtt agaactagga aagataccag tgatcgtcca
     4321 tccagaacgc aacgcgcatt ttcgagaaga cccaaatcat ttgcttccgt tcctagatat
     4381 gggatgtttg gcccaactta ctgcgccaag ttatgttggt gtatttggca aagatatcca
     4441 gaaaacagcc aaaacaatgg tcaaacataa tctcgtgcag atggctgctt ctgatgcaca
     4501 cggggtctct aagcgcacct tttacttaaa agagtgctat gagcagattg ccaaggagtt
     4561 tggtaaggaa aaagtagtac agatgaaaca agtcgcaaag gatctgatca acggggatga
     4621 gatcaattat ccaatctatg aagaggtaaa aaagaagaag tttggtttat tttagttctt
     4681 tattcgtaga taaaaaggaa atgaggagag agtgagagat gtatagaaaa aactcaagtg
     4741 gttggtctaa acatattgat tttattttat tagacttatt ttgtgtccag cttgcttttt
     4801 atatatcata tgtaatgcga cagggtgact ggaatccgta tgtgattcct atttatcgcc
     4861 acatggcaat ttttatagaa cttgagaatc ttacgattat atttttattt gaggcatata
     4921 aaaatgtttt aaaaagagga tattataaag aggctgtagc ttctataaag caatctttta
     4981 tgctgttact tgtgtgcagt ttatacctta ttacaatgca ggatggaaat gattattcca
     5041 gagtttcttt atttataatg ggggtggtgt atggattatt aacatatata attcgtatac
     5101 tttggaaagg gttgttgcat caccgcatga aaaacggtga agatgtatcg ttagttattg
     5161 taacatctca aaagattgca aaaaatgtag ttcgtaatct taaagagaag aattatggca
     5221 tgtataaact tagtggtgtg attgtagtag acagagatat gtatggaact accatcggag
     5281 gtgtcccagt agttgcaaat gctgataatg cagcggaata tcttttacag aattgggtag
     5341 atgaagtatt catcaatgtt gacgaaagtg ttccatatcc caaagaatta atcgccagat
     5401 gttcggaaat gggacttgct gtacatatga atcttacaaa agtgtctgat agtacacgtg
     5461 gaaaacaatt tattggccga gttggtgatt atactgttat tacatctagt attaatgtta
     5521 tgacgatgag gcaagcattt ataaagagaa cgattgacat tcttgctgga attattggat
     5581 gtattgcaac aggtgttatt tttatcttcg tagcaccagt tatttacatc aaatcaccag
     5641 ggccgatttt ctttgcacaa gaacgtattg gtcaaaatgg taagacattt aagatgtata
     5701 agttccgtag tatgtatatg gatgcagaag aacgcaaagc agagttgatg aaagaaaata
     5761 agatgagtaa taatctcatg tttaagatgg attttgaccc aagaatcatt ggcaataaaa
     5821 tacttcctga tggaacaaag aagacaggaa ttggacaatt tattcgatct acatcattgg
     5881 atgagtttcc acaattttat aatgtcctaa tgggaaaaat gagcctggtg gggtttagac
     5941 cttgcttgaa gtcagaatat gagaagtatg attttcatca tcgtgcgaga attgcgatga
     6001 aacctgggat tactggaatg tggcaggtga gtggtagaag tgatatcaca gacttcgaag
     6061 aggttgtaaa acttgatgca gaatatatca ggaattggag tatgggatta gattttagaa
     6121 ttttgtttag gacagtcaaa actgtgcttc atagaaacgg ttccatgtaa gataaagata
     6181 ctatagaagg agtaaaacgt gatggcaaga aaaaaattaa aaatctgtat gctaggtcat
     6241 aaaagaattc catcaagaga aggtggaatt gagatagtgg tagaagaatt ggcaacgcga
     6301 atggttgcac ttgggcatga agtgacttgc tataatcgaa gtggtcatca tgttagcgga
     6361 aaggaatttg accagcataa aaataaagaa tataaaggtg taaaattaaa gactattttt
     6421 acattaaata taaaaggaat tgcagcaatg agttcttcag tatttggtgg aattagagca
     6481 gcattcggaa aatatgatat tgttcatttt catgcagaag gaccatgtgc tatgttgtgg
     6541 ttgccaaaac tttttgggaa aagatgtgtt gcaacaattc atggtctaga tcatcaaaga
     6601 gaaaaatgga acaagcttgc tagtacatac atcatgttag gggaaaagtg tgctgtaaaa
     6661 tttgcagatg aaataattgt acttagtgaa agcgtccaaa attattttga agatatatat
     6721 ggaagaaaaa caaggtttat accaaatggc gttaaaaaaa ttgaaataaa atcagcagga
     6781 ttgattacag aaaagtatgg attaacaaaa gattcttata ttttgttttt ggggcgattg
     6841 gttcccgaaa aaggaattcg gtatctaatt gaggcattta aagatgttca gacagataaa
     6901 aaacttataa tagccggagg aagctctgat acagatgaat ttgcaaatga actaaaggaa
     6961 ttagcaaaag gtgatgagcg aattatattt actggatttg tacaaggaca ggaattagaa
     7021 gaattatata gtaatgcgta tatctataca cttccaagtg atttagaagg aatgccatta
     7081 agtttgttag aagcaatgag ttatgggaac tgttgtatag tttcaaatat ctcagagtgt
     7141 acggaagtag tagaagacaa ggctatgatt tttaagaaat cggatgtaag tgatttaaag
     7201 ataagattag aagaggcatg caatcaatcg gaaatggtta aagttttgaa gaatcaagct
     7261 actgagttta tttgtagtaa atataattgg gataaaatcg tacaagaaac attaaatttg
     7321 tataggggaa atatataatg attcgaatat tacagtgtgt gaatgatatg catcgtgcag
     7381 ggttggagac gatgctaatg aactattata ggaatataga tcgaactaaa attcagtttg
     7441 attttttgac acatcgtcca tataaaagtg attatgatga ggaaataata agtcttggag
     7501 gaaaggtcta ttatgcaccg agactttatc cacaaaatta tcctaaatat tttaaatgga
     7561 tggaacaatt ttttaaggat catccagaat atgagattgt tcattctcat attgatgcga
     7621 tgagttattt acctttaaaa gctgcaaaaa aagctgggat tccgatacgg atagcacata
     7681 gccataatac atcgatagat agagatttta aatatctttt aaagcaatat tatcgatgta
     7741 agttaacgtc tgttttgaca catgaatttt catgtggaaa agtagcaggt gattttttgt
     7801 ttcgaaatga taaattcact ttgattccaa atgcagtgga tgctaaaaaa ttttattatg
     7861 atgaaagtat tagaaatgat gtacgaatga aattaggaat ttcaagtcag acgtttgtaa
     7921 tgggacatat tggaagaatt tcatattcta aaaatcatcg ttttttgatt gaaattttta
     7981 aagaatttca cgctattaag aataattccg tattattaat tattggaact ggtgatatgg
     8041 aagaagaaat aaaaaattat gcaaagaaaa gtggcataga tgatgatatt aaatttttag
     8101 gaaatagaaa tgatatagaa aaattttatc aagcatttga cgtattaatg ttgccatcat
     8161 tatttgaagg ggttccgtta gtgggtattg aagctcaatt tgcagattta ccgtgtttct
     8221 tttcggagaa agttcctaca gaagttgctt tcagtgataa gaccaatttc ataagtttaa
     8281 atcaatcggc aaaagaatgg gcgagagaga ttgcaaatgt agatatctct catcgagatt
     8341 cagaacgttc gattttaata aaggcagatt ataatattca aacagcatac aaaatcttag
     8401 aatcaaaata ttatgagtta tttgaattaa tacaaagagg ttaaagaata tgacacagaa
     8461 acattcaagt ataaaaagaa ttatacatcg catatccgaa gaattgaatg caacaagacg
     8521 aattgcaaag ataacatctt ggaaggaagc atttgccacg ttacaagcta aaattgatat
     8581 tcaagtcatg aatcgtaatg gatataatga aagtaaaaaa cagaaggaac atttattaaa
     8641 aaaacatgag gtaatgctga agtattatga aaagactttt tcagattttt tgaaaacata
     8701 tgattatagt caaaatcaaa taattaatca atcaaaatca ccatattcta actgcatttg
     8761 ggtttgttgg tggcagggat tggatcaatc gccggaaatt gtaaaagctt gtgtaaaatc
     8821 aataaaacga aatgcaggga aacatactgt tataatttta acagaagata attatagaca
     8881 atatgttgat ataccaaaat gggtggaaga aaagaaaaac aaaggaatca ttacgagaac
     8941 aaattattct gatttattaa gattatcttt gctagcaaaa catggaggtt tgtggttaga
     9001 cgccacattt tattgtacaa aaccaatacc agatgagtat tttgatatcg atatttggtc
     9061 tattaggagg cctgattatg ctcacgcatc cgtagcatca ggatattttg cgggttattc
     9121 attagcatgt aatcaagagt ctagatggat gtttgctata attagagact tttttttaaa
     9181 ttattggaaa aataatgata tgatggtgga ttatttgatg atagattata tgattgtttt
     9241 agcacagaga ataaatcatg aaatagcaca gacatttgat aaaataccta gtaacaatcc
     9301 attatgtgac gaattgatta aaaatttaaa taatatatat gatgaaaaag tatggaactt
     9361 attaaagaaa gatactttac tgtttaagtt gacatggaaa caatctttta aaaagcaaaa
     9421 agatgataaa gttacttttt atgggatgtt ggtagaaaac aaattattat aaatattaga
     9481 ggtataaaaa gttatgaaac acaaggtaag tgtgatagta ccaatttaca atgttgaaaa
     9541 atatttaaga aaatgtgtgg atagtttatt gagtcagaca ttaaaagaga tagaaatcgt
     9601 attagttgat gatggttcgc cagatgcttc gggggaaata gcagatgagt atcaaaaaaa
     9661 atattctaat gtaaaaacaa tacatagaga aaatgggggg ttaggaccgg caagaaatac
     9721 aggaattgag aatgcgacag gagaatatgt ggcttttctt gattcagatg attgggtaga
     9781 aagtgatatg tatgaaaaat tatacttagt tgcgtccaaa gaaaatgctg atatagtagt
     9841 tagtggacat tgtgattttg cgaatgacaa agctatggtg aaaaagcaac atcctttgag
     9901 tgggaatgta tatgatacgc ctaatagtat taaaaatgta cgaaaaaatt tatttggaca
     9961 tagtccaaaa gatcaagaag tagaggcctt cccgatgtca gtatgtatgt ctttgtataa
    10021 taaaaatctg ctagataaat atagactaag atttgaaaaa attttatctg aagatacaat
    10081 ttttaacttg aatgcatatg atgtcgcaaa taaaattgct tttacagaat atacagatta
    10141 ttgttataga aaagaagaac aagagtctat tacaaaaagt ttatctccaa ataagcagac
    10201 ggaatttaaa gaatttttaa taactctaaa agctatagca tcgaaagaag atttagaatg
    10261 tcaaatcaga gcaaaacgta tggcaattga ttattgtagg ctttatgtgg gcttagttga
    10321 tgatgcaaat ttgggaatta aagagaaaag aaagtatata aaaaaattta ttgaagatga
    10381 gggattaatt caatgttgga agggatatcc tacaaatacg ctaccatttc aacaaaaatt
    10441 ttttcataaa atgcttgaat gtaagtgtta tacaatattg ttgttgttaa gtagaattag
    10501 aaagataatt aaaaatatga ccagaaaaaa attgtttata atattataaa ctaaaagtat
    10561 atcaaataat agaaattaac aagttacaaa aaaatattat aaagaataat attttctaaa
    10621 aagtaaacta tagtaatgtg gattatcacc ttccaacagt atataaaatg agcgtaaaaa
    10681 cggctatttt tgccttaaca acgatttttt tgtatacaaa tacgatatgc tcattaatta
    10741 ttggaatttg tagtgctgtt taaattaatg aaggtgtaat tatatgagtg atcattttga
    10801 aaaagtttgt tgagaagatg tgcttctagt catgtgatgg gagaaaataa tttaactgtt
    10861 agagtgaata gttaaattat tctggttata tgaaaaaagt agtttctaaa ataatcttgc
    10921 agggattaac ttaaagaaat tgagagaata gtttctttga aatttctata tacaatgaga
    10981 agctgaaatt tacttttatt tgatgactgg aaatagaaat taaatatatt gtattgttac
    11041 attaaagaaa acttgtatgg aaaaatcagt acaataccat tatttaatgt tcttcactta
    11101 aagcgcaaaa aatatcagtt tattaaactc gtatatagaa caaatatatg tagacggggc
    11161 gaaacgatga gtgtaaaaaa aatgctattt atggtaagta caataattgt attaacatcc
    11221 cgattttggg gaattaatac tagtttatct atgcgatatt ttattatggc aatatgggat
    11281 ttatatttta tgttaagcta tttttcttat cgtgagaaaa catataaaaa atacgttatc
    11341 aagaataact tttggttgac tataatgccg tttgtgatat tttctatcta tactctaata
    11401 atatggggaa ttggttcaaa tgcagaattt gaaaatttta taaagttatg ttccacgttg
    11461 ctttatttaa ttcttgcata tggttacgca tgtacagcat tttacttatt taaggcagaa
    11521 ggaattgatt ttattttttg gtcaggtgtt atttcatata tgagtggttc agttttttat
    11581 ttaattgtat cttatggttt aaatggttta atttcttata taaaaagtct tataacaggt
    11641 gaaacaaata ctgcaaattt tgctatggag gtacatgatt taacttttgc gatgggggtt
    11701 tttttccttt attacgtatt ttttgaaaat aaaaatacaa aaaaacataa gataaaaatt
    11761 atcgtatctt taattttaat tttcatggga ttgaagagaa ttgaactgtt agctttaagt
    11821 ggtgcaattc tagtgtatta tgttttatta aagtggggaa aaaagattca aatccgtgca
    11881 ttagtgtgta ctatttgtgc aactgttatt tcgttagtgt ttatttacat tatagataca
    11941 ggaattttgg cattgcttat gtcaaatttg ggcattacag atgacggtgc aaggctttca
    12001 ttttatctat attcatctaa attttatgaa cttggtttag gatatgtagg aacaggatgg
    12061 acatggtttt caaaatactt ttttaatctt tatttatcgc actttaggtt aaatggtgga
    12121 agaattgcag caagcctaca cagtgatata ttaactattt atatagaagt tggatgtata
    12181 gtttttatta tatggatgtt ttggttattt tttgtgaaac cgataagact aactaaaaaa
    12241 tttggattga aaacagggga gtgtgtatta ttattaacta tatatatgtt tatattatat
    12301 ttgacggata atacgctcac ttatccagac actcaaatga tttttctctt agtacctatg
    12361 atagtttcgt gggttgataa tcaacaacga atatcggaat agatagctaa agtatatttt
    12421 aattatgaat ttataattag ttgtaaaagt taatatttaa cggacaggaa gtagggtgaa
    12481 aaaatttgaa aaaaatttta gtgttagcaa atacatatta tcaaataata gtagcaatac
    12541 aaatgaaaaa tacaattttt tccgatgagt atgttgaaat tattatttct gatcattcta
    12601 aaaacactaa agaaattgcg aaaaaaatag aagaaaaaaa agtgtttgat ggctgtcatt
    12661 atttagaaag caaatctttt gatgataata ttgggtattt tcaaaagatt aaggactgtc
    12721 ttcagatttc ttgtggtaaa tcaaatcgat atgcctatta tttgaaaaac tgtaaaagtg
    12781 aatattttga tgaatttatt tgtttttcaa tgggagaggt ttggttggaa ggaatatatg
    12841 cttgcttata tcgaaataat aaaaatgtac aggtatcaat tttagaagaa ggtattttaa
    12901 gctatggagt tgacattatt ccaacagcaa gaacaaaagc tatcgcagta ctcaattact
    12961 taaaaaaaag gactccacta tataacaaaa gctataattt ttattgtttt tttcctaata
    13021 tatatacagg aagagagaat acaatacaaa ttccattagt tacagctggg tcaagaaccg
    13081 cattgcaact ttcggatatt tttaacccta tgatagatat ttataaagaa aaaaaatata
    13141 tattttttac gtctgtttat gattttgagg gcggagaacc tgtcggagaa tatgaattag
    13201 tatgcaaaat agcagactta gtaggaaaag ataatctaat tgttaagacg catcctagag
    13261 atgtaagaac aatttataaa gataatggat ttatcgagga taaaaattca tcaatacctt
    13321 gggaggcgat tcaattgtca ggcgatttta acgataaaat atttatgaca attaattcag
    13381 gaagtgttat ttcaggtagt acaatgtctg caaaacctgt acgcacgtat tatatgtata
    13441 aattgtgtaa catagaagga aatccgtctt gcaaaaagaa tgcgtatgat attgagcgat
    13501 tgcttaagca aaaaacgatg aaaaacattt taaagaatgt gaaaatagca gaaagcttag
    13561 atgatatact atgagcaaaa ctaaatattt aattaaaaat atgggaatat taacaataag
    13621 taatttttct tcaaaaattt tagttttttt gttagtccct ttatatacaa gtgtattatc
    13681 cacaacagaa tatggcacat atgatttgac aatttcaaca gccacattat tgtttccaat
    13741 tttaacctta aacattgtag atgctgtgat gcgcttttta atggataaag ataaaaataa
    13801 aaaaaatata gcatctatag cgattaaagt tatattttta agcgtgatag tctttggatc
    13861 cgtaatgtat ttgttaaata ttacaaaatt atggcaagat gtttatggat tagaaaaata
    13921 tatttttttg tattatatat cctatgtatg gaatcaattt ctaatacaat ttgctaaagg
    13981 gttagaaaaa gttaaagcta tgggcatagc aggtattatc agcacaattg taacaataat
    14041 tgccaatatt ttcttcttgc ttattctgaa atggggatta attggttttt tcattgcaaa
    14101 tattttatca cagattattt cagctactta tctgtcagta ttagtgagaa tatgggaata
    14161 tatcgatttt gaggctaaag atcctcagct aaagagagaa atgctaaatt actcgattcc
    14221 attaattgca actgttgttg gatggtggat taatagtaca gctgataggt atgtggtttc
    14281 ctttatagta ggtgtctcgg cgaatggctt attgtctgtg gcatataaaa tacctcaaat
    14341 aattaatact ttgcagggaa tatttataca ggcctggcaa atttctgcaa ttaaagaata
    14401 tggagataat gagactgcaa aattttatgg aaatacattt gttatgatta atttgttaat
    14461 gtgcataacc tgttcaagtc ttatcatatt aactagaccg ttttcacata ttctttatgc
    14521 gaaagacttt tatgaagcgt ggaaatatgt tccattttta ttaatagcta atgttttaaa
    14581 ttgtgcatca ggtctgttag gacctatctt atcagcaaaa aaagattcta aatccatgat
    14641 gtggtctgca atcatagggg cattagttaa tgtgattatg agtgtagtcc ttgtttataa
    14701 gataggggtt caaggtgcca caatagccac tgttatatgt tcatatgtaa tatatgttgt
    14761 tcgtaaacga gctgtgggaa aagatatttt tataaagcat tatcatattg ttgtggaatc
    14821 ttggggatta ttgtttgttc aggcaatcat agaaatatat tttaaaaatt atgtaataga
    14881 aattataatt atgattattt taattattat aaatatcagt tatattaagc agttgtgtta
    14941 tagaggaaga aacattattt caatgattaa gagtcgagag cacaaacggt agacgatgtt
    15001 atttttataa aaagatataa tttattaatg gtcgataatt atgaaaggaa aaatattctt
    15061 aaaaaaaaga gtataggatg tcgtatgaaa atagtcgcta tagtaccaat gaagttaaat
    15121 aacaaaagat tgccacaaaa aaacacgaaa gcttttacca atggaaaacc attgtgttat
    15181 tatatacttt caacactttt aaaagtaaaa cagattgata aagtgtatgt ctattgtagt
    15241 aacccagata ttaaagaatt tataccagaa ggtgtaacat ttttaaagag atctagttcc
    15301 ttagatcagg atacaacgag tattacacag gtattagccg gtttcacaaa agaagtagag
    15361 gctgatgtat atgtaatgac acacacaaca gcaccattta ttagcgaaaa atctattgaa
    15421 aaaggactag atgccgtgct gacaggagaa tatgattctg catttgctgc aaaaaaattg
    15481 caagatttct tatggaaaga cggtgaacca tttaattatg aattggacaa tattcctaga
    15541 acacaggatt tgccgccttt atacgaagaa actagtggat tttatattta taatcgcaat
    15601 gtgatgaccg agttgcatag aagaattggg aaaaaacctt gcatagttga agtaggtgag
    15661 attgagtcag ttgatattga tgaagccgag gattttatga ttgctgatgc gatttataat
    15721 catcttattt tacaaggtag ttaagacagg gggaaggtta ggatgaacaa tgtacagatt
    15781 ttagattgca cacttagaga tgggggctat tgtaacaatt gggaatttgg atttgataat
    15841 acaaaaaaaa ttatttctgg acttatagaa tcaggaattg agattataga atgtgggttt
    15901 ctcacaaata aaacagtata caataaaaat gttacaaaat atacgacctt agaagaatta
    15961 gcacagataa ttcctgaaaa aagagaagga aaattatttg tagcaatgat gaactatggc
    16021 gaatataata tagaggatct tccagattat gatggttcat ctattgatgg aattaggatt
    16081 gcgtttcata aaaaaaattg caatgaagcg ttgaaattat gtcgtgagat aaagaaaaaa
    16141 ggctacttag tttttgttca agctatggta tctctcaatt atacagatga agagttttta
    16201 gcagtaataa aaaatgtgaa tcagtttaaa ccatacgctt tttatattgt tgatagtttt
    16261 ggaatgatga aaggaaaaga tttaactcgt cttttttaca tgattgagca taatctagaa
    16321 aaagatatac agataggttt tcactcacat aataacatgc agcttgcata ttctaatgca
    16381 caaaaactta taaccgtaca aacaaatcga agcctgatta ttgattcttc ggtgtacgga
    16441 atgggaagag gagcaggtaa tcttaacaca gaactatttg tagaatattt gaatgataat
    16501 gcaggaaaaa aatatcaatt aaaaccatta ttatatataa ttgatgaaat actaaatagt
    16561 ttctatcaga agagttattg gggatattca ttaccaaact atatttcagc agttcataat
    16621 gctcatccta attatgcaag ctatctgtca tataagaata ctttaacaat tgaagcaatg
    16681 aatgaaatat tcgaaatgat ggatgaagaa aaaaaggtat cttatgataa acaatatata
    16741 gaagatttat actacaaata tatggaaact gggaaaatac aggaggaata tatggaagaa
    16801 ctgagaaaat ttctctcaga caaaaaaatt ctactgattg caccaggaaa aagttctgta
    16861 gatgaggtag aaagaataaa agagtttgtt accaaagaag aagtcgtaat aattagtgta
    16921 aattttgaat attctcatat agatatagat tacgtatttt taagtaattt aagacgtttt
    16981 catgaattac cgaaagaaag cagaactaaa tgtattgtta catcaaatat acaagcatcg
    17041 gatgtatatt tacggacgag ttataaaaag ttgttatctg aaagggaagt tgtaagagac
    17101 aatgcaggat taatggcaat tagattttta gccgatatga atgtggatga aatattctta
    17161 gctggatttg atggatattc acataatgaa gatgagaatt atggtgagca atccatggaa
    17221 attataacaa aatttgcatt attagatgca atgaacgtgc aaatgagaga tgaattgtca
    17281 gaattagcta aaaaagtaaa ggtgacattt ttaacggatc cacgttgggt tcgaattgat
    17341 aagtagtagt aataatgttt ttaaaatttt gataaagaga tagtgagaga ataaaaatgg
    17401 gtaaagtctt aaataaaaaa gaagaaaaat atttaagggc cgatggggcg agatgaaact
    17461 ctaattttga acttttaaga atcatttcta tattgtgtat tgttgcttac catgctaatt
    17521 tgcgtttaaa ttataatgca gacggaatta tagtacatgg ttcattaact gctaaccaga
    17581 ttttcttgaa ttatattggt atttggggtc agctaggagt taattgtttt atatttattt
    17641 cggcatattt tttaagtgag aaaagaaaca ttcactttga aaatgttttg ggaattgtat
    17701 tagaatgtat tttttatagt gtattagttt atggaattct ttgtgcatgt ggaatagaaa
    17761 cattttcatt gaacagcttt gtaaaagaaa tattatcatt tgctctaggt agatattggt
    17821 ttattacatc ctatttagca ttgtatattc taagtccgta tttaaatatc atgcttaaca
    17881 atctggaaaa acttcagttg caaaaaataa gtatgttatt gtttataggt gtatgctgtt
    17941 atcgtatgtt atttaaaaca tctttgtttg gggattgtgc tgttttaatt gcactatatg
    18001 tcatatgtta tacactgaaa aaaacgataa taatattttt gagagacaca gaatcatagg
    18061 aacaattgta tgtataacag ttataccact tctcatatcc ataggaatac ttttcaaatt
    18121 tataccaaat gcaatggtta accagatggt tcatattggt tctccattga tggttatagc
    18181 aagtatattt ttattttatg tatttaaaaa tttttcattt tacagtaagt atataaataa
    18241 acttgcgaga accagtttag gggtatatat tttacatgaa aatcattatt tcattgatgg
    18301 tatgaagtct agaggcttaa tatgggatgg gatatttgga ttaggcaaaa ggtttttaga
    18361 tagggatttt tggatttggt ttataattgc agttgtagtt gtgtgcataa tttgcatggc
    18421 tatcgatgtt gtaagagaaa aatttgaaag acagattttt aaaattaaga gtgtaaagaa
    18481 cctgtgtaaa cttatgaatc atgttataaa tatataccca taattaataa atgaggagaa
    18541 aagaagtgaa agttatatca ttcggagaat tattattgag acttgcagca cccggttatt
    18601 caaagttatt tcaaagagat agtttggaga ctacattctg cggaggggaa gctaatgtag
    18661 cggtatcctt agcaatattt ggatatcaat cagaatttat aacaaagctt ccggattcag
    18721 atgttggata tgcggcagct aatgctttgc gttattttgg agtaaatgtt gataatatta
    18781 tatttggaga gggacgcatg ggactgtatt atttagaaaa aggagcagct cagagaccat
    18841 ctaaagtaat ttatgacaga aaattttctg caatttcgtt agctgatagt gatgaattta
    18901 agtggaagga gatcctaaac gatgcaaatt ggtttcattg gacagggata aatccagcat
    18961 tgtctgagaa tctctttgag acgtccttag atgcatgtca ggctgccaaa aaaagaggta
    19021 taacaatttc ttgtgattta aattatcgat ccaagctttg gacatcagag gaagcaaaag
    19081 aaaagatgga acagatacta ccattggttg atatttgtat ttgtaatgaa gaagatgcag
    19141 aaaaagtgct aggaattaaa gctgagcata caaatattaa tagtggagaa ttaaataaag
    19201 atggctatgt gcaggtggct agggagattc acaagaagtt tggatgtagg tatgtagcta
    19261 caactctgcg tagaagttat tcggcaagta gaaatggttg gagtgcaatg ttatacgaag
    19321 cagaaaaaga taaagcgtat ttttccacag aatacaacat acaaattgtt gatcgcgttg
    19381 gtggtggaga cagcttttct gctggtataa tatatgcgtt atgtgaaaat atgtccggac
    19441 aaaaggctat tgaatttgcg actgcatcta gttgtttaaa acatactata gaaggtgatt
    19501 ttaatcgaac tacaattgag gatgtatata atttaatgaa tagcggtgga aatggtcgcg
    19561 tgttgcgtta gtcaacatat aaagataaat cacagtaatt tttatggtga ttatctgtct
    19621 tagtagtgat tagaataacg tgaaaataaa ttgttgagcg tttatactgt tttgaaaaat
    19681 aaatttaaca tattgaaaaa tacaaatata tacgtatatt ataaagcgcc gattgtaaaa
    19741 ttaagctaga caactaaaac agtcatttgt gctacactca aaatagttcc gaccaaagaa
    19801 ttataaggag tgagtgcaaa taacctatac ctatcttaca acggatgaac ttgtaataat
    19861 agagtcttat ttgaaaatta accaatctgt tgctaaaact gcgcattgct tgaatcgttc
    19921 aagtcaaacg atccataaag tatatctgtt cttcaagcaa agaaaatcag ccttagaata
//
LOCUS       DAYLZO010000050        19878 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.051, whole genome shotgun sequence.
ACCESSION   DAYLZO010000050 DAYLZO010000000
VERSION     DAYLZO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.051"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..782)
                     /locus_tag="ACGVJG_001603"
     CDS             complement(<1..782)
                     /locus_tag="ACGVJG_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFJ6557546.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFR"
     gene            complement(896..2227)
                     /locus_tag="ACGVJG_001604"
     CDS             complement(896..2227)
                     /locus_tag="ACGVJG_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFJ6557547.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(2193..3671)
                     /locus_tag="ACGVJG_001605"
     CDS             complement(2193..3671)
                     /locus_tag="ACGVJG_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFJ6557548.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(3976..5811)
                     /gene="tdc"
                     /locus_tag="ACGVJG_001606"
     CDS             complement(3976..5811)
                     /gene="tdc"
                     /locus_tag="ACGVJG_001606"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFJ6557549.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            6281..7708
                     /locus_tag="ACGVJG_001607"
     CDS             6281..7708
                     /locus_tag="ACGVJG_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557550.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(7800..8165)
                     /locus_tag="ACGVJG_001608"
     CDS             complement(7800..8165)
                     /locus_tag="ACGVJG_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFJ6557551.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(8162..8527)
                     /locus_tag="ACGVJG_001609"
     CDS             complement(8162..8527)
                     /locus_tag="ACGVJG_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFJ6557552.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(8719..8967)
                     /locus_tag="ACGVJG_001610"
     CDS             complement(8719..8967)
                     /locus_tag="ACGVJG_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557553.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            8966..9094
                     /locus_tag="ACGVJG_001611"
     CDS             8966..9094
                     /locus_tag="ACGVJG_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFJ6557554.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            9151..10845
                     /locus_tag="ACGVJG_001612"
     CDS             9151..10845
                     /locus_tag="ACGVJG_001612"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFJ6557555.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            10945..11241
                     /locus_tag="ACGVJG_001613"
     CDS             10945..11241
                     /locus_tag="ACGVJG_001613"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557556.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            11619..12752
                     /locus_tag="ACGVJG_001614"
     CDS             11619..12752
                     /locus_tag="ACGVJG_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFJ6557557.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            12783..13103
                     /locus_tag="ACGVJG_001615"
     CDS             12783..13103
                     /locus_tag="ACGVJG_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFJ6557558.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(13132..13596)
                     /locus_tag="ACGVJG_001616"
     CDS             complement(13132..13596)
                     /locus_tag="ACGVJG_001616"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6557559.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(13727..14980)
                     /locus_tag="ACGVJG_001617"
     CDS             complement(13727..14980)
                     /locus_tag="ACGVJG_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFJ6557560.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(15009..15326)
                     /locus_tag="ACGVJG_001618"
     CDS             complement(15009..15326)
                     /locus_tag="ACGVJG_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFJ6557561.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(15352..15672)
                     /locus_tag="ACGVJG_001619"
     CDS             complement(15352..15672)
                     /locus_tag="ACGVJG_001619"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFJ6557562.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(16136..17551)
                     /locus_tag="ACGVJG_001620"
     CDS             complement(16136..17551)
                     /locus_tag="ACGVJG_001620"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFJ6557563.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(17568..18884)
                     /locus_tag="ACGVJG_001621"
     CDS             complement(17568..18884)
                     /locus_tag="ACGVJG_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFJ6557564.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(19034..19732)
                     /locus_tag="ACGVJG_001622"
     CDS             complement(19034..19732)
                     /locus_tag="ACGVJG_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFJ6557565.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         6304 a         4097 c         3359 g         6118 t
ORIGIN      
        1 cgaaatgtcg taaaaataac taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt
       61 ttgtttaatc ggttgatttt tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat
      121 gcttgattga ttttaccgat ttctgtcgtg tttcctgttt ctacaacgac accgactgca
      181 gaaccagatt gtacaagcgt tcctgaaaaa cccatatttt tctggtcgcc gatcgattct
      241 tcttctgtca aaacggcatg acttttttca gatgcttctg attctcccgt caaaatcgat
      301 tcttccacta ataaatcatg tgcttcgatt aatcggatat ctgctggaac gacatctccg
      361 gcacgtaaga caatgacatc tccttttacc agttctttcg cagggatttt ttcttgcatc
      421 ccgtttgata gtactatcgc ttcttgtccc atcagctgct tcaaaccatt caatgattct
      481 tccgcttttc tttcttgcca gtaactaacg agtccattga cgatcactac gaataaaata
      541 atacttcctt caatatattc actagaagca aatttcaaaa aagaggcaat gatcaacacg
      601 atcatcagca gatcagtaaa atgctttgct atttttttcc atcgttttgt ctgctgcctt
      661 gcttttattt cattatatcc ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag
      721 ccttctgttg atgtttgata gtttttcaat acatcttcta ccgtcaatct gtaccagttc
      781 atcctatccc ctcctgttta attgatactg ttattatacg cagggaagtt ttttcaggcg
      841 tatcgattat ttcagggggg tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac
      901 tgatatttct attgcttatt cttttatata caaaatttaa gaatacaaac cagataacca
      961 ataaaaaagg aataaagcgt gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa
     1021 aaaacaccaa caaagcaaga tctttatatc tttgtgtagt gttccgtttc ttttctttaa
     1081 taaattgaac atgtgcacac aagatcgaag accacacaaa tagaaaacaa ctcgttgtca
     1141 tgcctgccaa aagctgaaaa aacaatcgac tatcagctac aaaaaaatgt agacaaagac
     1201 cggcaaaaaa taacattcct gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa
     1261 gcgcacaaaa ccaatggggc aaactttttt cttttgctgt ttgatataaa atcctgctag
     1321 tactgtagac tgcactattt gctgaggaca aagaagcaac caaaatcatt agattgacaa
     1381 ttgctgctcc gccttggcaa tggatcactt gcagcatagt cactaaagga ctttctttta
     1441 aatctaaaca atgccatgga acaacaaata aaatcgacaa aatcgttcct atatagaaaa
     1501 agagaatacg ccaaggcaga cagttgattg cttttttcaa attcttttgc ggatcacttg
     1561 tctcgccaac ggttaagccg atcatttcaa ttccagcaaa actgaataaa accataggaa
     1621 atgagactac cattccagca aagccaaacg gtagtaattc atcgatctgt ccaaaatttt
     1681 ctttgattcc gatagactgg gtatgaaaat ctgagacact caatcctata cccaataaga
     1741 taaaaaggaa aatccccaag atcttgatga ttgagaaaaa agattccaat tcaccaaacc
     1801 acttgactgc aacaaggttg actgccatta agatcattag tgcgctacct ccgcttatca
     1861 aagctggtac atgtacaaac cagaaatgga tatacaatcc tattgctgtt aattccgcaa
     1921 gtccagctcc aagccagcaa agccaataag tccaattgac aaaaaatgcc cactttttgc
     1981 ctaaataata gtcaacgaaa tcactgatag agttaaatgt actatttgct aaaaataaat
     2041 cacccatggc ccgcatcaaa ccataaatag ccaaaccgca tatcaaataa acgagaataa
     2101 cagctggccc tgctgatgca attgcctttg ctgaacctaa aaataatcct gttccaattg
     2161 cgccacctaa tgcgattaac tggatatttc gattagttag agtccgtttc attgccggtt
     2221 tttccatcaa gttcaccttt ttctttttca agcagcatgg cgacttcctg ttcccaagaa
     2281 ggtttcttta ttttttcaat acagagtggt actacaaaca aaatgacact gatggctgtg
     2341 actgcaatta gattgatcca tgttccagct ttcatcgtca gtccgacaga aaatccgatt
     2401 gccgcaaata aaacaataac gacagtccaa actccaagat tgctcttttt ctcttttttt
     2461 ccaattctaa atggtcgatt caactttgca tgtgtataac gcaatctcaa aactgcgatc
     2521 cccatgatga catagacgat acagtagata acagtagttg aatttacgag catcaaaaat
     2581 gcttgattga cgccaggaat cagcaaataa aaaagtgcaa agattgagat aatgacagac
     2641 tgagtcaaaa taacggattt ggatacgtca aattgattcg tcttccaaaa attgaatttt
     2701 ggtggatacg caccttttct agcactttct gtcactgtct ttgctggtcc tgaggcccaa
     2761 gcagataatt ggactgctac accaatgaag acaagtaaac taaacagatt cacaataaga
     2821 tgtggcaaac caagaatttg gcaataaatc gaaatagatt gcgcgatatt attcaattcc
     2881 atttgtcctt ttggtacgac atttgctaca actaaagcat tcaaaatatt gacgaaaaat
     2941 ataaaaatta acgcggcaaa tactccgcga atatacgttt taactggatt ttccaatcga
     3001 gtgatataca cagaggacat ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa
     3061 acaaatgagg atgcagtcgt agtatctggt attaatttgt cccatgaaaa actgcctaat
     3121 gttccggttg gtacgatccc aactttgatc cacgcagcaa accctaataa gagcatcatc
     3181 gtcaaaggca cgtacaatcc taaccaaaca ccgacttttc cgccgatttt tgccatatca
     3241 aatttcaaat tcaaaaaagt gatgatccaa taaacgacta aaatgacaac taatgtaaat
     3301 ttgctgttca aaccaagcgg cacattatcg ataatattac caaacaacgg agcgagtgct
     3361 gaagcgacca tcaccatccc aggaaacatt tggacccata acagccatga gacaacgaac
     3421 ccccattttt ttcctagagc attcgatacc caaagttctg gaccaccttt ttggggaaac
     3481 attccagcaa attcaccaga gatcaatgag attggaaaag cgtacaataa tactgcaacc
     3541 agcatgtaaa aaatcatcgt ccagctcgtt gctgctacat ctggaatatt tcgtacactt
     3601 gctacaaggg cgcaggtcat tccgataaag gtcatggtcg tgatttgctt tgatgaacca
     3661 gtatctttca tctttattcc ttctttcaat agcgacatat gtttttgttt tcttttattt
     3721 ttgttcaaga acagaaatga caaggtttca tcgattcagt acaaaaccca gtatcaccga
     3781 aacatccatt gaacgatctt agtgaactgg gtttgataca tgaaacgaac gatccaactt
     3841 atagaacgtt ttataaaaaa gaatatcttg gcgaggaata tatgaaaaac agggggaaat
     3901 acaattgttt tgatgaagca aataattgtc tgttctccat atgtctcttg ttattctttc
     3961 acacaaaaat cagttctatt ggatgatttc gtttagtttc ttttccattg ctttggcgat
     4021 tttttttgta taaaaattga aaattcgatc atcattcaaa taaggagtca tgatagccgc
     4081 acgtagcaga gtgattttct gttctttttt ccattcttct ttcgttagac ccattctttc
     4141 aacaaaacga atcggagaat caccgtaatc ttcttgtgta aatgtagtat gagaagtaat
     4201 aaaccgttca ccataaacat cgccatccat ataagaagac cgatcgaaca ttttttcatt
     4261 taattcattg atagcgttca aatctgtaca gccttgttct ttgaataccc aattgaccat
     4321 attaaaatca ggatgattca atggatagac ttcgattgtt tttcctttaa cagtaaaagt
     4381 cagatgatcg agaaattctc tgaatctttg agctgcctcg attgaagccc caatcaattg
     4441 accatatccg gtcacattca atggcaatac acgatgtgct gtccagactg cggcggcggt
     4501 tgctccagct tttgatcctt ccaaaatata agctcctaac atatctggag cctttactga
     4561 tttttcaaac acatatggcg cgaaataaga aatgatattt ctcatatttt tatgtttgat
     4621 tacgattcct ccagcagcat aagggacata gcccatctta tgaggatcta tcgtgacaga
     4681 atccgcttct gaaatagctc tgaacccttc atatacctct ttgtcaatcg ttacgcagtg
     4741 gtggaaaaca tgatgttctt caaagaattc agctaaggaa gcatacggca caaattctcc
     4801 tgcttcgttt agaaacagtg aacgcgcata gccgccatat gcggcatcaa catgtaaata
     4861 gaaatagatc ccttcatctt ttaacctttc tcttaattgg acgattttat ctacactgtc
     4921 tacttgacct tcttctgtcg ttcctacgac agcaaccaca cctagaattg ggattttttg
     4981 tccagctaat tcacgaattg ttttttccaa agcattaata tccattcgat aatcctcttg
     5041 aactgggata gcaaccatct gatctaagcc gactccgcaa atatctaacg ccttcatcca
     5101 agaatagtgt tttgtttgag ggaccagcca tttccctaat ctttggatat tttttccgct
     5161 acgggaagaa gctgctttga cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc
     5221 caaaatttct tcaacagata aattcaaaag ttcccactca gacatttttt tcactttttc
     5281 aggcagcact tccttgacag ctaaaggaat cgatttgatg caacgggcgt accataatcc
     5341 ttcaagatta gcaatagaac catctgctgc aatgtgtccc catccgtctg tcatattgaa
     5401 taatgaagca aaatcctgtc ctacttctgc ttccatttgc gaagttgcca tagaagattc
     5461 caatgcaaca ttatttgggt tccacagcat cgcatagttg taagctaata aagcaggcat
     5521 caatgtttca gcattcattt gtccccagta acggcctgct gaatgccatg ggatcgatcc
     5581 tgcgcgtaat cgctgactga cttcatccaa gacctctatc atatggtcgc gggtggctag
     5641 ataatctgga gtgagcttat ctccttcgct aattgcaggc atatctgaag gaatataatt
     5701 ttctctccat ccaagatgtt catctaccat cttgttcaac aacattttat aaactggtcc
     5761 attttcagct ttgtcgccta taaagacggc cttgatatcc atatctttca tttttcttcc
     5821 ctccatattt tgttggtcaa tgtcttgcag atacatgtgt ttatctgtaa cacctcttca
     5881 ctatagcgcc aaaaatgaga attttttttt tataacatca aatccacgta gatttttaca
     5941 aacaaaaata aataaagtcg gatttttctg ataattcaac gcaaaaaagc gaacaatttt
     6001 tcaataaatc agtcttctaa aatagataac ctaaaaaaag cgaacaaaca aataaaatga
     6061 ctgtccttca ttcactaaac atatttttat ttttttatat tgttaaaaat tcgcaattta
     6121 ttttttaata gaattatctg ttttccaaat tttataacat aaaaaacctt ttttaacggc
     6181 atttatatgc tttttcagaa attaactgat gatttcaaaa agaaaacatg tcataatatt
     6241 tcccatattg tgcaaaaaaa tacaaggcgg aggctctact atgattgaag aatatattga
     6301 gaaaaatatt ttaagacagc tgtttttatg tgggcaattc tatgtaaata aagaagtcaa
     6361 tctagaaaag ctctccaatc tcctgcatgt ttgcaaaacc actttactca atgatatcaa
     6421 taacataaaa aaagaatttg aagaacagat cgcttataca catcgggaaa aggaccgtta
     6481 cactttatat ttttcagaac atatacctcg atgcaagatc atgcagcaat tgtctcaaaa
     6541 ttcgttgttt ttaaaaactt gtctgcttta tttggaagaa gatgaacctg attatcttca
     6601 attaactgaa tgtgaattca tctctgtcag caaagcttat tcattaaaaa aacaagtatt
     6661 agcttatttt aatgactgcg ggatcgaaat cgaccgctac tcccctcgat tcactgaaat
     6721 ggaaagacgt ctgctgcttc ttaatgtgtc gtatcgttta ggcggtttta attcttggga
     6781 gctacctgaa tcgttttttg aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag
     6841 tggcagattt tatgataaag aaaataaaga aatcttatcg attggttttg ctatcagttt
     6901 cctacgccag caagtgtgtg ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa
     6961 acgtcctatt tacaattatg tcgaatcagc atgggaaaac actgatttcc aaacctatta
     7021 taaaaaagaa gaattcgcct ttattttgac actattcaat ctttgtaatt atggtttcca
     7081 ttcttatcag ttgattgcgg aagactttca acagctacac caagtgttta ttgacaatac
     7141 tcccgaaata aaagaactag ttgctacctt tgaatctcac ttcaatcaag aattattcgg
     7201 aaaccaaccg ttcgaacgtg cgctgattca tctgatgcgt tcggcttggg ataactatca
     7261 attattcatg ccagaaaaat tttatctttt aaatgaggaa cagacaaatt tatataaaga
     7321 agtccagaca attttttcca gctggtccag ccagttacct tatgatctac ggttgaatcc
     7381 taattgcatg cgggcttttg tgatcgaatt gagcggaatt ctccgtctga caaaagaaca
     7441 tctgactatc tatatcgtca caaattcgga tgttcattat ctgatttatc gggaagcctt
     7501 ggaagctgta actactttcg attttcaagt tgcaccaaca atttattcaa gtatatctga
     7561 tataaagaag tatgcgcagc agtcctcaaa ccgggtatta tgtgaacgta cattatatac
     7621 accagatgcg gtccaatatg aaaatattat tcctatttcg atcaatacga tcgatcgagc
     7681 tatcatttca gctgtgcaaa acaaataagg ggctgtgaca aaaatcatgt cacagcctct
     7741 tatttgttga agatgcccat ttctgtaaca atctcttgat acacgtcagg ctcacaacct
     7801 cacttatttt ggacatctac gggtccgatc tctttattta aaaaataaga aatgatggtt
     7861 cggataacta ccaacgccgc tagttggaaa atatcctgca aggtaggttt gataatcgat
     7921 tcgatgatcc cagctgaaac aagaatttcc aaacttagaa gtatatagcc tcccaacatc
     7981 tttttaatag cattgttctc gctgactcgg tcgctttgca cttttgtatg gacaaataaa
     8041 tatttcaaaa aatccttgat tgatagacaa acaccccaaa ccaataaaca gatagcaaag
     8101 atatcaagtc ccagtacaag tttttcgaaa tatggaagaa gaaactgcat caactctgtc
     8161 gtcatcctac ctctcctctt ctttgttttt ctttgtcttc tatttcctga ttcaagaaat
     8221 aagaaatgac tgttcgaatg ataataataa gtgcaagctt caaaatatct gtccaagtcg
     8281 gcttgataac tgactcgatg atatcagcaa cgactagtac ttccaagctt aacaaaatat
     8341 aacttgctag cagttttctt gtatctgcat tacgagtatt acgttctgca tagtttttct
     8401 tgacatgaga ccaatcaaaa agtttgaata gaacaagaac gatcccgaca attaagatac
     8461 cgatagaaca aatatttaaa atagcaatga tccattctag aaattcaatt aaatttgagc
     8521 gaaacatata cgctacctac tttcttttaa tttgttccta ttcttttatt gtaaaagaaa
     8581 gttaccttgt tcgtctattt aaaagttgtt ctaatgataa cggttcgctt aaatttttct
     8641 ttttcactct cccatttttc tccctccatc cataaatcgt ggaagtatcc aaaacaacgt
     8701 agcaagttaa aataaggctt agtccgtcat cagcttttca aagaagcact gtctcggtgc
     8761 tttttttgta cccaaatttc taaaaataga accatacacc tcctcaaatc ttttcttctt
     8821 acctacccta tttttattta caggaaaatc agacacttct tcttttttgt caaaatttcc
     8881 tttttcttct cgtcctatca tttggcattc taataaaaac gaactatcct tggatataga
     8941 aagagaaaac aaggaggcgt ttttcatgag taaaaaatta atcaaagtcg gcatcggttt
     9001 aggtttgctt gcccttggcg ctgcatatct aggaaaaaag acaggactat tcgaagatga
     9061 cagtcattta tatgatgaat tcgaatcaat ctagcatcct atttagttga atcactacgg
     9121 acatacttgg aagacattgg aggaatcaca atgagaaaaa gtaaagcaat aatgattggt
     9181 gccggtattg ccaatatggc agcggctgtt tatttgattc aagaaggcaa atggaaaggg
     9241 gatcagatca ccttttattc attggatgat cacggttcaa atgatggtgc accgaccgac
     9301 actgtagtgg atgaatattg gaataaaaac catccattag aaaaccaaaa aggctatatt
     9361 gctcgaggcg gtcgcatgct taattaccgt acctatgtag atttgatgga tctgttaagt
     9421 cgaatacctt ctgttactga gccagggatg actgctgaag aagatacgag agattttgat
     9481 gcaaaacacc gaacctttga caaagcacgt ttgatggaag gtggcaaagg aatcattgat
     9541 ggcggaaaac tgggattcaa taataaagac cgtattcttc tgactaaact gattgctatg
     9601 cctgattcag aagaagaaaa actagacaat atcactatcg aagaatattt caaagatgat
     9661 ccacactttt ttgaaacaaa cttctggttt atgtgggaaa caacatttgc tttcagaaca
     9721 cgtagttctg ctcaagaatt gcgtcgctat atgcatcaaa tgatttatga attcactcaa
     9781 atcgaacatc tagtgggcgt caatcgaaca cgttacaatc aatacgaaag tattatgctt
     9841 cctttgatcg agtacttgaa agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact
     9901 gactgggaat tcaaagaaac accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt
     9961 accaatacac tgactcaaga agaagaatac gttgtggtcg atgaagatac agcagttatt
    10021 ttcactaatg ggtccattac agattcagct actttaggag attacgaaac accagctgta
    10081 gaaaatatgg attatggtgc agcttctagt ctctggaaaa aagccagtga acgtttctac
    10141 aacttgggaa atcctgacaa attctttgct gatcgagatg caagcgaatg ggtcagcttt
    10201 accctgacga cgaaaaacca tttgttgttg aatgaaatca cccgtatcac gactcaagaa
    10261 ccaggaaatg ccttgaattc atttatttca accacaccga tcacgccgtt aggccaaaaa
    10321 gatgtcaata tgtctatcgt cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat
    10381 gaaacagtta tttggggtta tttcctttat ccaagacgac gtggcgaatt tgtggataaa
    10441 cagtatatta aaatgaatgg aaaagaaatg ctagaagaac tgattggaca actttcaaaa
    10501 gtagatcctg gaccagtgaa tattcgtgaa aaagaaacag aaattttcga cagcgtaata
    10561 aataatatcc ctgtctatat gccttatgct tccgcgttgt tcaataatcg tgccaaaagc
    10621 gatcgaccaa aagtcattcc taaacattcg accaatctgg cctttacagg agaatttgtc
    10681 gagcagcctt atcagatgat ctttactgaa caaagtgctg tacgttctgg agaaatcgct
    10741 gctttccatt ttgcgggtat tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa
    10801 gatattccta ctctagctcg tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa
    10861 aaagctgccc tgcatcaagt aataagaaca accaggctaa aacagcttct tatgtttttg
    10921 atttggttgg acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttgt
    10981 gaaaaagctg ccctgcatca agtaataaga acacccaagc taaaactgct tcttatgttt
    11041 ttgacttggt tggacttatt accgcagatg caagcttttg aacaccattt accaagaggt
    11101 tatgaaaaag ctgccctgca tcaagtaata agagcagcca agctaaaaca gcttcttatg
    11161 tttttgactt ggttagactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga
    11221 ggttatgaaa aaggcgttta gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc
    11281 acgtttttgc ataaatttga cttaataccg agaaactgat cccttctgtc ataacctctt
    11341 tttatttatg tctgtttctt tttgaaaaca actatttttt caacgtttac tctcttgcca
    11401 atccaacatc atctaacgga tacaactatt tcaatgatta ccattacact aaaaaatcgg
    11461 aaacacacct ctccgaacac aaatacataa gactatagaa tgtttcattt aagaaaaaaa
    11521 gcgtttcatt atcaaaaagg taagatctcc tttaaaatga aattatctgt agtactaata
    11581 atgcagacaa ttttatgaat ctgaaaggag catctccaat gaaagcagtc acttggcaag
    11641 gaaaacagaa aatggaaatc agaaacgtag atgatccaaa aatattagaa ccaacagatg
    11701 caatcatccg tatcacagca acagccattt gcggctccga tctgcatctt taccatcatg
    11761 gtgaagcagt tttggaaaaa gggtatgttg taggacacga accaatggga atcgttgaag
    11821 aagtgggatc tcaagttcaa acattgaaaa aagggcaacg tgtggttatc ccttttaata
    11881 ttagttgtgg tcattgtcat ttctgcctga atcacatgga aagccaatgt gatgaatcaa
    11941 atccagatca gttatatggc gggcttttcg gatttggcaa gttgaacggt aatcattggg
    12001 gtggtcaagc ggaatatcta agagttcctt atgcagactc tacttcattt gttgtaccag
    12061 ataatgatct tcctgatgaa aaagttttat ttctatcaga tattttgccc acagcttatt
    12121 ggagcgttga aaacgcagga gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc
    12181 cagtcggtct attcgctcaa aaattcgctg ttatggctgg agcagagcgc gtaatcgcag
    12241 tagatccagt acagcaccgt ctagataaag cggctaatta caatggggtt gaaacttatt
    12301 tcttagaaga cacggcacaa gctggaacag aattgtatga actggctaaa ggtggcgcag
    12361 atgtcattat cgattgtgta ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa
    12421 atttagtaag cttacaaagc ggcacaatct ctccattaca gatggcatcg caagctgtca
    12481 aaaaatttgg aacagtccaa gtaactggtg tttatatgac tccagcttct tcttacccgc
    12541 tgcaagaatt ttttatgcgg gatatcgctg tcaagcatgg acaggcacca attatccatc
    12601 taatgccaaa aatttacgca atgattgcag aaggactatt cgatcctaca caaatcatta
    12661 ctcattcgct gccactatca gaagcagcac aagcatatga gatctttgac aaaaaagaag
    12721 ataaaaatat aaaagttgtt ttgaaacctt gaattcagta aaaaatacag gtgattttct
    12781 ttatgcccaa aatcaaacga aatacccatg agatcgaaat aggaaaagaa gaagatatcg
    12841 aaatacgagg acgtcgcttc cgcttctatt tccaaaatcg ttacacattg atctcactgg
    12901 ctgtcgattt attaaccggg attttctata tttttggaag ccttgcttcc ttaactaaaa
    12961 tccccgatcg atatggcacc tatttttatt tggctggtgc tattttcttg acgattcgtc
    13021 ctattctcag aatcgtaagg aacattttta tttacgacga aaaaaaagca atgaagagaa
    13081 caagcaaaaa aaaagaggca tgagaatttt tcaaatctca tgcctctttt ttcaccctat
    13141 cgtatcactt tgatacagac gagcggtcgt cccactttct tccttgacca ttgtcaaaaa
    13201 tgtccactcg tatcgttcat aaaacgtggt atgatctgta attaagtaaa gtttttcgta
    13261 cccaagttga tgcatttctt ttctgatttc cgctaatatc cgcttggcta tcccctgctt
    13321 acgcttttcc ggttcaacaa acagggcaca aagatttggt gtcagatcag ggcggttatg
    13381 gaagtcgttt tcaatgacgc ccgctcctcc gacgatggtt tctttttcat ctaagaccac
    13441 ataccattga ggaatacttt ccttattagc gattccagta cgaatacttt cctcatacac
    13501 aacagcagga actccccatt ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg
    13561 gtgttctctt aatttgatcc agttatatgt aaccaatgtt taaacctccg actaaaataa
    13621 aacgtttaca aaaattatac aagtctttat gaaaattagc aatgacaaca atagtgatgt
    13681 aaaaaaaaaa cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag
    13741 cttcttttct tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag
    13801 aaagaaccaa attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac
    13861 cgccaataat tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg
    13921 tagaagtcgc aaagtaagca acactaacta agaccattgg tgtcaaaata aatggaatga
    13981 acatgatcgg gttcaacacg ataggtaacc caaacatcgt tggttcatta atattgaaga
    14041 cacctggcgc aatcgacaag tttgttacaa ccatatatgg tttgttttta cgtcctacta
    14101 aataaatagc taacagtaaa cctaaagttg cacctgttcc tcctaagttg acaaagctgt
    14161 caaagaaagg tttgttcacg ataaacggtg cgctatgccc agctgcgatt gcattaacgt
    14221 ttgcttcaat ggctggcgca ttgattgttt gcatcaaagg atcgaccata ttcgctccgt
    14281 gtaaaccaaa gaaccataag aatggtgtga taaatgcaag cagtaaggca gatggataag
    14341 agtttgcaag tcccataaat ggttcttgga ccactgtata gaaagaacct acgatatcat
    14401 caacaccgaa tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata gtgatcattg
    14461 ctggtagtaa tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca ggcattttga
    14521 tgatcaattt ttcatttccg cttaaacgta caaagatttc tgttgaaata atcccaacaa
    14581 taatagcaat aaacaaaccg tttgagctca tacctaatgc tccgcctaaa gcgaagaaag
    14641 agcctaaaga aatcacacct gcggaaacac cgtcttttcg gtagccgtta gctaaatgat
    14701 aagcaagcaa gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc caagcagcag
    14761 ccccgaatcc tttccatgat tcaccaccaa aaattgagtt catcaattct tggaacccgg
    14821 gaatcggtaa gttattgatc attactgcta atgctcctaa gatcattaac ggcatactca
    14881 ccataaatga gtcccgaatc gcaactaaat gccgttgtgc accaattttt gatgctacag
    14941 gtataaaatg tttctccata aatgcaacaa atttatccat aaatcttccc tcttttcttt
    15001 actgtagatt acgcgttcaa tcgacgatac agatcaatga tctcttttgc taaatctgta
    15061 aaagctatag atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg
    15121 tgatttcctt gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa
    15181 gattcctctg catctttgat tttttcatct gctaaagtga aatcaccttt tttcgcagca
    15241 gcaatcgctt ccattgcatc gctttttgca tttccgccaa acatgatcag acccatgatg
    15301 gcttctagat ttttttgatc ttccatttat tgtatcctcc gataattttt attatttgtt
    15361 tattagtttt tctgcttggt cgagtacttt ttcaccattc atcatgccat agtcagccat
    15421 attgattaca tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt gattttgcat
    15481 aaagcgtact tgcggtccta aaagtaaaac gtctacattt ttagattcta gttgattatc
    15541 cgcatctgaa gcagatacag caaagatgtc tgcttccatt ccttttgctt ctgctgcctt
    15601 ttgcattttt gtgactagga gacttgtact cattccagct gaacatacaa gcatgattgt
    15661 tttcttttcc attttaaaca cgttcctttc tatacttaca cttgtatata agcaagtagc
    15721 gtgccaactt taaaagcgct ttaaaaagtt gatatcatca catttaaggt ctataccaag
    15781 ttgcatacac actaaaaaag aaagcgcaca ctaaacacac aaattaattt ttattacctt
    15841 ttttctttta acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa
    15901 taaacggtat tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag
    15961 atgttgtttt tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct
    16021 caataaacgg tgttcaatca gctgaccctg ggtattagct caaaacgctg gattcacaac
    16081 ctcttttatt tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt
    16141 tatagatttc gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag
    16201 cagatttttt caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc
    16261 gattcttaaa tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt
    16321 cgcaatttgc gccttcttct gttgctttga tcaagtaatg caaatgctgt ccgataaatt
    16381 cgatacggta atcatcttgc acgattcctt tctcatccat atactgttct tcatattctc
    16441 ttcccatacc gttttctgtt atcagccaag ggatattatg ataattttct tttagataca
    16501 tggctatatc atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct
    16561 tcccaggtaa atcaaagact tcataatatc gttcaggatg gaaaggtgtc tcttcattcc
    16621 attgataacg aggggcgcga acgcgtttag gataatattg attgattcca aggaaatcta
    16681 ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt
    16741 tttgacacaa ctccaggaac tcttctgtgt agtatccttt taccatcgga tcaaaaaata
    16801 ctcgattata aaacaaatca tacatataag cagctttttg gtcttctgga gaattggatc
    16861 gagcatagac catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc
    16921 ggtaaatctc tactgcttta gccgttgcta aaactttatg ataattccac atcatccact
    16981 tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa
    17041 cgattggttc gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa
    17101 tttgagcata cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt
    17161 ccaaaagaaa agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt
    17221 cgatcaaaga gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt
    17281 tctcataatc agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga
    17341 tggatttcat cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg
    17401 ggccataccc attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat
    17461 cctgtccttc tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa
    17521 gaataaaatt ttttggaata gtaattttca tgatttcctc ctgtttttta gatcccatca
    17581 ttttccacac gatttgctac ttttacaaat ggcagataga tcaaaataga aacaataata
    17641 caaacaagtt gcgtaataac tgcaccgata tttcctgctg ttgccagata agcattgacg
    17701 atcggcggca ttggccaagg gaccattaca actgctttcg ccgcaaatcc taaagcggtt
    17761 gcaaaataac caatcgttcc agtgataaga ggcgttaaga taaacggaat agctaaaatt
    17821 ggattcagca tgatcggcat accaaaaata actggttcat tgatgttgaa gatcccagga
    17881 gcaagggata acttggtgat ttgtttcata tcttcccgtt ttccaactaa caatactgca
    17941 atcagtaaac cgatcgtgac tccagacccg cccatactca tatacatatc ccaaaatggc
    18001 atagtaataa tgttcggtac ttcttttcca gcttcaaaag cttccgtatt tacagcaatt
    18061 gccgctaaaa gcaacggttc ccgtatcggc ttcaccattt gatttccatg gataccaatc
    18121 acccaaaaga cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca
    18181 ccttctaatg ggcgttgtac gacattgtaa atgatatcat aaaggtacat tcctgtaagt
    18241 tctttgatga taaatcccgc agccgcgata cccgatacag tcagaatcgt cgggaataaa
    18301 gcagaaaaac tagcagaaac attcgttggc acggtatcag gcattctcag ctgtagtttc
    18361 ttctgtttcc tcaaccagca ataaagttca atagaaagga tggagaccaa cattcccaaa
    18421 aataaccctt tcgtatctgt atattgttta gccaatacgt tttctactat aacagtctga
    18481 ttttcattca ctaattcata aacagttgga ttaacagata aataagacat cactgcgatg
    18541 ataccaggga atacaccatc cactttattc agcttggcta attcgatccc tatcagaaaa
    18601 acagcataga tggtcaagaa acttagtgtg acatagctga tggcagatgc gataggcttt
    18661 aattcttcta aaaaacgcaa gaaacttatc tgtgctaagc cattttccga gtcaaagacc
    18721 attgctgaaa acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat
    18781 gcgtttttta tcaccatgat ataccttaaa ttattgattc tcgttgccac gttccctaaa
    18841 acattggcta atttatccat aaaactcatt ccagatcccc tcattcctgc ttcttctcct
    18901 tgacgtcatc tctttttgtc tattagtcag cgcttacatt tcgatgaaca gaaactattt
    18961 actcctcttt tttaccaaat ataaaacaaa aattcatctg atgtatttat cataaaatac
    19021 atcagatgaa ctgtcaatta gtttcttttt ctcctattat tttctgtaat tctctccgat
    19081 ttattgccat atggacagtc attgcatcaa acgcagcaag tgcattccgc ctttcgatgg
    19141 cctgaacgat ctctgtatga atatcgatcg tctcgatttt cagatgctgg atatcataat
    19201 ttttattaaa caaagcaatt gattcattaa tgattgggat aatatgcagc aaggctacgt
    19261 ttccgctagc ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg
    19321 tgccttctcg aaaagaagtt tcaatctttt gcgttaattt tttcatttgg acgatttgat
    19381 tcttcgttgc atattccgct gccaaacctg ccatcttagg ctccaataaa tatctcagtt
    19441 caaataagtc tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat
    19501 tggcaatccc tcgcttatca ctgacaaatg tccccgctcc ttgccgaact tcaaggatat
    19561 tcctggaagc tagagcccgc acagcttctc gcaccgtgct tcgccccacc tctaaataat
    19621 cggacaattc atattcattc ggcagtttat ctcctacttg caactgcatt tcctttaaat
    19681 atctaaggat cttgtcagta gttacttcta ctagggtttt attttcttcc aaaccacata
    19741 cctccttttc atctgatgta ttttttcgta attattataa cacgtttgaa tcatctgatg
    19801 attttttgta tacaaaattg attaatccgt tcatagacat acaaacgaag aagatattta
    19861 ccataaaaaa gactagga
//
LOCUS       DAYLZO010000051        19843 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.052, whole genome shotgun sequence.
ACCESSION   DAYLZO010000051 DAYLZO010000000
VERSION     DAYLZO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.052"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1209
                     /gene="polA"
                     /locus_tag="ACGVJG_001623"
     CDS             <1..1209
                     /gene="polA"
                     /locus_tag="ACGVJG_001623"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFJ6557566.1"
                     /translation="RKMELPLSIILAEMEISGIKVDATRLQEMKGEFSARLREIEQKI
                     YEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVLEQLREQAPIVEDILT
                     YRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVDPNLQNIPIRLDEGRK
                     IRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEGQDIHASTAMRVFGIE
                     KAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQYIDTYFEKYPGVKEYM
                     ERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAINTPIQGSAADILKIAM
                     IELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEVMEQAVSLHVPLITDS
                     SWGKTWYEAK"
     gene            1254..2090
                     /gene="mutM"
                     /locus_tag="ACGVJG_001624"
     CDS             1254..2090
                     /gene="mutM"
                     /locus_tag="ACGVJG_001624"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFJ6557567.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            2054..2683
                     /gene="coaE"
                     /locus_tag="ACGVJG_001625"
     CDS             2054..2683
                     /gene="coaE"
                     /locus_tag="ACGVJG_001625"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFJ6557568.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            2735..3067
                     /locus_tag="ACGVJG_001626"
     CDS             2735..3067
                     /locus_tag="ACGVJG_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFJ6557569.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            3379..3870
                     /gene="nrdR"
                     /locus_tag="ACGVJG_001627"
     CDS             3379..3870
                     /gene="nrdR"
                     /locus_tag="ACGVJG_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFJ6557570.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            3893..5266
                     /locus_tag="ACGVJG_001628"
     CDS             3893..5266
                     /locus_tag="ACGVJG_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFJ6557571.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            5266..6192
                     /gene="dnaI"
                     /locus_tag="ACGVJG_001629"
     CDS             5266..6192
                     /gene="dnaI"
                     /locus_tag="ACGVJG_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFJ6557572.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            6321..6761
                     /locus_tag="ACGVJG_001630"
     CDS             6321..6761
                     /locus_tag="ACGVJG_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFJ6557573.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            6905..7606
                     /locus_tag="ACGVJG_001631"
     CDS             6905..7606
                     /locus_tag="ACGVJG_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFJ6557574.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            7603..8724
                     /locus_tag="ACGVJG_001632"
     CDS             7603..8724
                     /locus_tag="ACGVJG_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFJ6557575.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            8739..9971
                     /gene="pepT"
                     /locus_tag="ACGVJG_001633"
     CDS             8739..9971
                     /gene="pepT"
                     /locus_tag="ACGVJG_001633"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFJ6557576.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            10103..11011
                     /locus_tag="ACGVJG_001634"
     CDS             10103..11011
                     /locus_tag="ACGVJG_001634"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFJ6557577.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            11046..11318
                     /locus_tag="ACGVJG_001635"
     CDS             11046..11318
                     /locus_tag="ACGVJG_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFJ6557578.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            11329..12375
                     /locus_tag="ACGVJG_001636"
     CDS             11329..12375
                     /locus_tag="ACGVJG_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFJ6557579.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            12626..15235
                     /gene="clpB"
                     /locus_tag="ACGVJG_001637"
     CDS             12626..15235
                     /gene="clpB"
                     /locus_tag="ACGVJG_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFJ6557580.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(15386..16072)
                     /locus_tag="ACGVJG_001638"
     CDS             complement(15386..16072)
                     /locus_tag="ACGVJG_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557581.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(16050..16544)
                     /locus_tag="ACGVJG_001639"
     CDS             complement(16050..16544)
                     /locus_tag="ACGVJG_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFJ6557582.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(16564..17784)
                     /locus_tag="ACGVJG_001640"
     CDS             complement(16564..17784)
                     /locus_tag="ACGVJG_001640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6557583.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            17931..19766
                     /gene="lepA"
                     /locus_tag="ACGVJG_001641"
     CDS             17931..19766
                     /gene="lepA"
                     /locus_tag="ACGVJG_001641"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFJ6557584.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         6672 a         3593 c         4241 g         5337 t
ORIGIN      
        1 cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag cgggatcaaa
       61 gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt aagagagatc
      121 gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc aaagcaatta
      181 ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa aactggatat
      241 tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt agaggatatc
      301 ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact gctgaaagtg
      361 atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca aacaggacga
      421 ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga agggagaaag
      481 atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc tgactattca
      541 caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa agctgccttc
      601 ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat cgaaaaggca
      661 gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg tatcgtgtat
      721 ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc agcacagcaa
      781 tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga aagaatcgta
      841 cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg atatctgcca
      901 gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc tatcaataca
      961 ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt ggataaacga
     1021 ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga acttgtcttc
     1081 gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt tatggaacaa
     1141 gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac gtggtacgaa
     1201 gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa aaaatgccag
     1261 aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta ggaaaaacga
     1321 ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca cccatctttg
     1381 aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag tttttaattt
     1441 ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa tatcaattta
     1501 cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt gaggatggaa
     1561 gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc gaaaaaggag
     1621 caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca gatagcttct
     1681 tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag ccattgctgc
     1741 tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca ttatgggaag
     1801 cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta gaacaattac
     1861 gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc acgattcgca
     1921 gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat gtctatcagc
     1981 aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa gtggcacaaa
     2041 gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag gatttgtttt
     2101 aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct ttcagtctca
     2161 tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc ctggaacgct
     2221 tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg atgggcaact
     2281 agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc aaaaacttga
     2341 tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct ctctaagttc
     2401 caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt atgaccacta
     2461 tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc gtttgatgaa
     2521 acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac ttccaatcga
     2581 agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag aaaaacttgt
     2641 gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg tgtcaatttt
     2701 gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa atcaaaagga
     2761 taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag aaaatctaat
     2821 cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt accaagcaag
     2881 aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag atttgactca
     2941 agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa gtgaccacat
     3001 tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa tcgaagagaa
     3061 gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt gttcatctgc
     3121 ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt ctcagtaatc
     3181 aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa ttacgcatga
     3241 aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc gtttgtgatt
     3301 tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata taaaatgcga
     3361 tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc acgagtaatc
     3421 gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg tgagaattgt
     3481 tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt aatcaagaaa
     3541 aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat ccgttcagca
     3601 gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt cgaaaaccgc
     3661 gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata tgtgatggaa
     3721 gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta tcgccagttc
     3781 aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc aaaagaaaat
     3841 aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt ccttggaaaa
     3901 tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg ctccgctgag
     3961 agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg gagaagcctt
     4021 gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag gtcctgaagg
     4081 gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt atgatgcaag
     4141 aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata gtgatctggg
     4201 acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt tcaaagactc
     4261 tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga agcttaccca
     4321 attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa aaaaattttt
     4381 agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca tcgaatcgct
     4441 gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt cgacattcga
     4501 ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg atgatccaat
     4561 catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga cacttggcga
     4621 actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag aactgcaaaa
     4681 agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag ttgcggtttc
     4741 tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc ttcagctgat
     4801 aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt cagaaaaagg
     4861 cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc aatcaggtct
     4921 ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga ataatgctag
     4981 cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga aaatcgccac
     5041 agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac aatcaaaaca
     5101 aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa agcttcctga
     5161 ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag cagagattga
     5221 ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga agacgtaggc
     5281 aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc tgaaatgatc
     5341 gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca agaagaattg
     5401 acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca agaaaagcgt
     5461 aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa gctgacgttg
     5521 aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc acggcaaaga
     5581 caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat tcaatcggct
     5641 tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga agccctagat
     5701 tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta tctagtagga
     5761 agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt agcgattgct
     5821 ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa acaggcaatc
     5881 ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat tttgatgatc
     5941 gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt cggtgttatc
     6001 cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt tacaatggat
     6061 gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct aaaagcgaaa
     6121 cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg tcgcaaccga
     6181 cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta aaagcaggtc
     6241 agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag taatgcagaa
     6301 acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta gttggtctat
     6361 tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt gattcgattg
     6421 atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc atatttgtcg
     6481 gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag ttgaaactcg
     6541 tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc aatgacggag
     6601 tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg aaaatcggtg
     6661 acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc ccaccgcaag
     6721 ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca ttttttcaaa
     6781 aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga tatgggaatt
     6841 tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta gaaggagtga
     6901 agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact ttgtccctga
     6961 atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg cattgatatt
     7021 aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc
     7081 acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc ttgcaaatgg
     7141 tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg gtatgggagg
     7201 ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa gcggacaaga
     7261 acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc tgcaagaaaa
     7321 tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat
     7381 tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga tgttcgggcc
     7441 atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag agcttcaaca
     7501 aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac gtcaaaaaca
     7561 aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat cagcggacgc
     7621 actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga aggagaccca
     7681 gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat gacattagac
     7741 gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa
     7801 catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca agaaaaaatg
     7861 tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa catggatatt
     7921 atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt ggagagttat
     7981 ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc tgtggatcat
     8041 gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg tgattatacc
     8101 ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg tgcacaacca
     8161 gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga ggttattttg
     8221 ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc ttatgaggaa
     8281 cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta
     8341 ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa agaagcgttc
     8401 caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt
     8461 gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca aagagcagat
     8521 gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag cgctgggttg
     8581 attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt tatcgagaaa
     8641 tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc tgaaacaaat
     8701 acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat cttttgccac
     8761 gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa acgactccat
     8821 cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa gagttaggct
     8881 tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta ccaagcaatg
     8941 ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca gattttaatg
     9001 cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg atcgtactag
     9061 atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa aattatgaag
     9121 gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac aaatcaggga
     9181 ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc cctcacggta
     9241 ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat aaattcgatg
     9301 ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta ggtgagttgc
     9361 agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa aatgtacatc
     9421 cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat tttcataatc
     9481 agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc ttccatttga
     9541 tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc gatcatgatc
     9601 gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag ttgaatcagc
     9661 gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac atgaaagaaa
     9721 tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc gagcttggta
     9781 tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg
     9841 gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga ttcgaatttg
     9901 tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca gaattgaaca
     9961 caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca atgatggaat
    10021 gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc ctcttttttg
    10081 aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca aaagttcagg
    10141 gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc
    10201 agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa
    10261 gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg gcacgattca
    10321 tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat tacttccagg
    10381 tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag aagaagctgc
    10441 tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca atgatgaagt
    10501 cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga tcagtcaaga
    10561 agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt tattgctgaa
    10621 gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag gaaaatccca
    10681 actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg acgactcagc
    10741 gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata ttttccgatt
    10801 tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg gcattactta
    10861 tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga
    10921 tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag gattaacagt
    10981 ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa gttggaagaa
    11041 gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt gtgaattttt
    11101 acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc tggcgtgttc
    11161 ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga ttgatctctc
    11221 gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc gctgcaggtg
    11281 tacttgttga tttgattctc atttattttt tccattaagg agcaggagat gtcgtctaaa
    11341 ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt tgccattatt
    11401 tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc taccagtgtt
    11461 gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga agggcgctca
    11521 atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg gttcttgacc
    11581 tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct gatctcggag
    11641 atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt gacagcactc
    11701 catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt ggtcatccaa
    11761 ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc tggtatcctt
    11821 ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa tgttcaaata
    11881 ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa atgggtggat
    11941 acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat ccaagacagg
    12001 actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg gtatgtactt
    12061 ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac catttcgttt
    12121 atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat tggttccttt
    12181 gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc agtttcttcg
    12241 cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt tgtgtatgtg
    12301 attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa agagaataaa
    12361 gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa agaaaaacgt
    12421 tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac aatcaatttt
    12481 gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg tatattttga
    12541 ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata agtgatctta
    12601 cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga cgcttcaaga
    12661 agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata tcgacattgc
    12721 ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct atacggatgc
    12781 cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg agtatccagt
    12841 tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct ttcgattatt
    12901 gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa cggaagtcgt
    12961 tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga ataaaaacgg
    13021 attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag aaagagtgac
    13081 ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg atttagtaca
    13141 gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa tccgtgatgt
    13201 catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag aacccggtgt
    13261 agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag atgtgcctga
    13321 aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg ctggagcaaa
    13381 attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca aaaaaagtga
    13441 tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag gaaaaacaga
    13501 aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg aattacattt
    13561 gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca aagcgttgga
    13621 acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga tcagtattct
    13681 gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg acaacgcatt
    13741 agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac cggacaaagc
    13801 aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact ctatgccaac
    13861 ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag ctgcactgaa
    13921 aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag aattagctga
    13981 acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag aagaagtcaa
    14041 ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag aagatgcaga
    14101 aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac ctcaattaga
    14161 aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga tggttcaaga
    14221 gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta tccccgtaac
    14281 gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac ttcataaacg
    14341 agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc gttcacgggc
    14401 tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac caactggtgt
    14461 cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag aagatcatat
    14521 ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc tagtcggagc
    14581 tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg tcagacgaaa
    14641 tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg tcttcaatat
    14701 cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag tagtagattt
    14761 caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc tggaaggcgt
    14821 gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc tattacgtgg
    14881 aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta caccattgag
    14941 cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac aacgtctgga
    15001 acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg ctgaaaatgc
    15061 ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg aagtagaaac
    15121 accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag taacgattac
    15181 tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg tctaatcttc
    15241 cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc tccttttttc
    15301 aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt cttgaagctg
    15361 cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat ttataacttt
    15421 caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt aaggcagcac
    15481 ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt ccgacaaaca
    15541 gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg tcgcataaga
    15601 attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg aataagagga
    15661 acaaccaagg actggttttt gatatctcag catctttcat caagtaacca atcaatccat
    15721 agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc caaaaagaga
    15781 tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag aaaaaccagt
    15841 aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg acttgtggca
    15901 tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct tgagaagtcg
    15961 aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca ccaaaagcaa
    16021 gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc atctaaaaat
    16081 ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt caatttgatc
    16141 aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc ttgtagtgca
    16201 gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa gccgatcact
    16261 gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc gatcgtcagc
    16321 ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc agcaagagca
    16381 atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc ttgaggaaga
    16441 tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg gttatataac
    16501 aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac atcatccttt
    16561 ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct ctagtgactt
    16621 cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag cacggcgata
    16681 attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc ctataggttc
    16741 atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac cattattcca
    16801 aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa caaacggaaa
    16861 ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt cgatctgctc
    16921 tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa tatctccatg
    16981 tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat ttcgagcttc
    17041 ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc agtagaacag
    17101 aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct catcttcatt
    17161 ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac catggttttg
    17221 ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta atcgctcaac
    17281 cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa aggaaagttt
    17341 atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag gatacaaata
    17401 agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta gatcttgcat
    17461 caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac ccattttata
    17521 acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga tcagtttatt
    17581 ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg aacggataaa
    17641 agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt ggaacacata
    17701 ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta attcaagatt
    17761 ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga taacagtata
    17821 gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt aaattactgc
    17881 ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct atggatataa
    17941 agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct catatcgacc
    18001 atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg acgtctcgcg
    18061 aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga atcacgatta
    18121 aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat atctttcact
    18181 tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc cttgctgcct
    18241 gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa acattggcca
    18301 atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat aaaatcgacc
    18361 tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc ggaatcgatg
    18421 ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat attctcgagc
    18481 agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta aaagctttga
    18541 tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg atcacggacg
    18601 gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca tttgatgtta
    18661 cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg gtcggtgatg
    18721 tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg gatacagtaa
    18781 cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg aatccgatgg
    18841 tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg gaagctttag
    18901 aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct caagcattag
    18961 gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc caagaacgct
    19021 tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt taccatgtta
    19081 ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct gaacctgtaa
    19141 cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca aatgattttg
    19201 tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg atggattatt
    19261 tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt gtctttgact
    19321 ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat gaaatgtctg
    19381 gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa aaagtcgatg
    19441 cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca atcgttgaaa
    19501 aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca gctattggac
    19561 aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg gctaaatgtt
    19621 atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa gggaagaagc
    19681 ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc gttctgaaaa
    19741 tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt aaaaaataaa
    19801 ccatgtttac atggatttgc caccggtttg ccaccggtgg ttt
//
LOCUS       DAYLZO010000052        19685 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.053, whole genome shotgun sequence.
ACCESSION   DAYLZO010000052 DAYLZO010000000
VERSION     DAYLZO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.053"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(56..1087)
                     /gene="queA"
                     /locus_tag="ACGVJG_001642"
     CDS             complement(56..1087)
                     /gene="queA"
                     /locus_tag="ACGVJG_001642"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFJ6557585.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1280..2455
                     /locus_tag="ACGVJG_001643"
     CDS             1280..2455
                     /locus_tag="ACGVJG_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFJ6557586.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2459..3100
                     /locus_tag="ACGVJG_001644"
     CDS             2459..3100
                     /locus_tag="ACGVJG_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6557587.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3097..4014
                     /locus_tag="ACGVJG_001645"
     CDS             3097..4014
                     /locus_tag="ACGVJG_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFJ6557588.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4011..4682
                     /locus_tag="ACGVJG_001646"
     CDS             4011..4682
                     /locus_tag="ACGVJG_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6557589.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5223..5711)
                     /locus_tag="ACGVJG_001647"
     CDS             complement(5223..5711)
                     /locus_tag="ACGVJG_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFJ6557590.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5912..6178)
                     /locus_tag="ACGVJG_001648"
     CDS             complement(5912..6178)
                     /locus_tag="ACGVJG_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFJ6557591.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6178..6441)
                     /locus_tag="ACGVJG_001649"
     CDS             complement(6178..6441)
                     /locus_tag="ACGVJG_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFJ6557592.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6962..7102
                     /locus_tag="ACGVJG_001650"
                     /pseudo
     CDS             6962..7102
                     /locus_tag="ACGVJG_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7244..9067)
                     /locus_tag="ACGVJG_001651"
     CDS             complement(7244..9067)
                     /locus_tag="ACGVJG_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFJ6557593.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9665..12370)
                     /locus_tag="ACGVJG_001652"
     CDS             complement(9665..12370)
                     /locus_tag="ACGVJG_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFJ6557594.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12676..13623)
                     /locus_tag="ACGVJG_001653"
     CDS             complement(12676..13623)
                     /locus_tag="ACGVJG_001653"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFJ6557595.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13813..14082
                     /gene="rpsN"
                     /locus_tag="ACGVJG_001654"
     CDS             13813..14082
                     /gene="rpsN"
                     /locus_tag="ACGVJG_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFJ6557596.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14258..14620)
                     /locus_tag="ACGVJG_001655"
     CDS             complement(14258..14620)
                     /locus_tag="ACGVJG_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFJ6557597.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14631..15752)
                     /gene="alr"
                     /locus_tag="ACGVJG_001656"
     CDS             complement(14631..15752)
                     /gene="alr"
                     /locus_tag="ACGVJG_001656"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFJ6557598.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15768..16118)
                     /gene="acpS"
                     /locus_tag="ACGVJG_001657"
     CDS             complement(15768..16118)
                     /gene="acpS"
                     /locus_tag="ACGVJG_001657"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFJ6557599.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16248..17759)
                     /locus_tag="ACGVJG_001658"
     CDS             complement(16248..17759)
                     /locus_tag="ACGVJG_001658"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFJ6557600.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18081..19433)
                     /gene="murF"
                     /locus_tag="ACGVJG_001659"
     CDS             complement(18081..19433)
                     /gene="murF"
                     /locus_tag="ACGVJG_001659"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFJ6557601.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
BASE COUNT         5435 a         4180 c         3713 g         6357 t
ORIGIN      
        1 cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca taaccttatt
       61 tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc gcatgctggt
      121 aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact aaagtcgatt
      181 ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag ccaggcgtaa
      241 tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc gttccaatcg
      301 tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca tttttacgta
      361 cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta tgcatatggt
      421 gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca
      481 gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc gtaaaatgca
      541 atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt tgatagcgat
      601 ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc aatgattcaa
      661 gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg tgttccaatt
      721 cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc ccaactttgg
      781 cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc gttagaagaa
      841 ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca ggcagaactc
      901 gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt tcatggaaat
      961 gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa tgatcgcggt
     1021 cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa tcttctgttg
     1081 ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg tttttcgttc
     1141 tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata aaaaaactac
     1201 cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct tcattgaaaa
     1261 tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg
     1321 aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca tttgttttat
     1381 cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa tgactgatcc
     1441 aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc cagtggaatt
     1501 gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca tgacgatccg
     1561 tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac ggaataaaac
     1621 agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc gctacccaca
     1681 tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca
     1741 agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc gtgattcctt
     1801 gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca
     1861 tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt
     1921 gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga
     1981 gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag tcggcgaagt
     2041 tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag caatcaaact
     2101 gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg tattaaaagg
     2161 atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat
     2221 tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata ccttgcaacg
     2281 cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg
     2341 tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg gagaagatcc
     2401 cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg tctgacctat
     2461 gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg aacatcttta
     2521 tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg gtgtcttact
     2581 gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct tgcagacaat
     2641 cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta aattacctgc
     2701 gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt acattgggat
     2761 gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga ctgagttcca
     2821 atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg gtattcgttt
     2881 agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg ctggtggttt
     2941 aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt tggcaggtac
     3001 gatccctgta acaattttag cactattggc tgattggctt ctaggtctct tggaacaccg
     3061 gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact aaaaatgacc
     3121 atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct tgccagcaat
     3181 acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca gatcttagca
     3241 agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat catcaacaat
     3301 ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc aatttcagcg
     3361 gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga aaaagatccg
     3421 aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca aacgtggttc
     3481 ccgtcctatg gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa
     3541 taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt agctggtgta
     3601 gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga aacttatggc
     3661 ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc agtcgaagca
     3721 ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag ttatgatcta
     3781 gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc agttgtagac
     3841 aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt aggtcatacc
     3901 atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct agtggaacca
     3961 tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa gtgaagcaat
     4021 tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat ggctcctata
     4081 tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc tttgcagcag
     4141 ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca aactgggtga
     4201 ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc attttaatca
     4261 ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat tccctcttac
     4321 cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc cttgatgtcg
     4381 gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta ccactctctg
     4441 tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg ataaccgcga
     4501 tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga accaatgcaa
     4561 cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg gcgatcatca
     4621 cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt acatcacgct
     4681 gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa ggctttttta
     4741 gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg ggcaaagagt
     4801 tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc aacgggttca
     4861 tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc caatccattt
     4921 tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc tgcttgttct
     4981 ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc cttttccatc
     5041 agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt
     5101 aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg ctaaaaacat
     5161 atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc ggtacacgat
     5221 tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt caccagaaga
     5281 gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt ggcgtaaaag
     5341 atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga aagcgccacg
     5401 actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt ggatacccgc
     5461 tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc aaaagaccat
     5521 gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat tgcttttttc
     5581 aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc ctcactttgg
     5641 cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat tttactgatt
     5701 ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc cattcctaaa
     5761 ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa tcatgcaata
     5821 aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga gactctttgt
     5881 taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag gcagaataaa
     5941 tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc
     6001 tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat
     6061 atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa ttcgtttgct
     6121 tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag ttactcatca
     6181 aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc
     6241 tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca
     6301 gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa tctcgatttc
     6361 agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag cagtgctagg
     6421 attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta tagcgtactt
     6481 gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt ttcctctttt
     6541 ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca cttcttactc
     6601 ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca tctttagcaa
     6661 aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag gttcctcgtt
     6721 gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag atttgtaacg
     6781 gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg attgcctctt
     6841 tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg ttgggtttgt
     6901 gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac ttgacgaggc
     6961 tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg aagaagctga
     7021 aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc aagacatcaa
     7081 aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc tgttcccatc
     7141 tttaatggct tatagagggc acctttagct gagaataaga caaaacagac cctctagctt
     7201 caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc ttcaaatggt
     7261 agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt aaccgcaaac
     7321 ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga ggaatcaaca
     7381 cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca taacggataa
     7441 ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca tctgggaaat
     7501 gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct agcgaaacat
     7561 gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc
     7621 gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt agttttgcat
     7681 cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc ttataaaaaa
     7741 tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac cgatagacaa
     7801 acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca atcgatttgc
     7861 ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga gataaagcaa
     7921 atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag ccatggaaaa
     7981 taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa tagcctagtc
     8041 gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa
     8101 gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc gctgtagcaa
     8161 attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt tgagacaaaa
     8221 cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg atccccacga
     8281 agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt tttgctcttg
     8341 gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa gaggaagaac
     8401 cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc gctcctggaa
     8461 gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca acaacgatca
     8521 gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat gcagattccc
     8581 tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa atagcgactt
     8641 gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct gagacagata
     8701 ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag ccagcattct
     8761 ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa gcatggatga
     8821 tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg gcaatgggta
     8881 gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt tgttccgtac
     8941 catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca aatcgggtta
     9001 atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac ctttttaagt
     9061 aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag tcgaaaattt
     9121 atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa
     9181 ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa tacagcaaaa
     9241 atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat agaacttgac
     9301 ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag atgtaaggct
     9361 gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa cctcttgata
     9421 aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa aacataagaa
     9481 gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta caacctcttg
     9541 gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc aaaatatgag
     9601 gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt cacaacctct
     9661 ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc attttttctt
     9721 cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa ccgatcatga
     9781 tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc cgtaacaagt
     9841 ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc cctgagactt
     9901 ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata atagcgatcc
     9961 ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg aacgctaagg
    10021 cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact cctagtaaga
    10081 aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca
    10141 tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt
    10201 attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat agtgcagtat
    10261 aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg gtttgtagct
    10321 caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat aatttgatga
    10381 cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct ttttggatac
    10441 cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc acagctgttt
    10501 cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca ttttttagtg
    10561 ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga atgatcggta
    10621 attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta tccatatttc
    10681 cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt tctttttgat
    10741 atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg agaagcgaat
    10801 caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga
    10861 cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata ccagcaatga
    10921 tgccattgat attattaaca acggcatcaa tttgatctag caattcatca atagccttcg
    10981 tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga ttgcttagca
    11041 agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt ccagcacttg
    11101 tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt tgagatattc
    11161 cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca gtcagcttac
    11221 catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc
    11281 caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca tctttcagct
    11341 gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct aaatttttca
    11401 catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt gcttcatcga
    11461 ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct gctagttgct
    11521 taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt tttgcttgat
    11581 ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc tcatctactt
    11641 tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt tcaggtagtt
    11701 gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg
    11761 ttgataaaat catatcttta actttattga tacttactag gttcgtatcg atgtcgtaac
    11821 ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat tcatttgtga
    11881 tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc gcattgattt
    11941 tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta aaactcagca
    12001 gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg gaacggactc
    12061 cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta tttttatgaa
    12121 gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct gctggcttgt
    12181 ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc cataatgctt
    12241 tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc agaagtgttg
    12301 caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc gtatttttga
    12361 ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac catgtatttc
    12421 atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat cacggcaatt
    12481 gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct ttactttatc
    12541 atgaaataaa agggaaagat aattttatgc caaagttttt atctattata cgtatacgca
    12601 aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt
    12661 ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag gaggatgtcc
    12721 ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat tcattgaaat
    12781 aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg cttctgcatc
    12841 tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt gccgcaaatc
    12901 tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga tctggatgcc
    12961 ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa
    13021 ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta atttttttac
    13081 cttttcctca tttttactgg atgcactggt taagattcgg attccctttt catggagaag
    13141 ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag tatctgtcgg
    13201 ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg tgaccattcc
    13261 ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg atggcaacac
    13321 gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac ctacatgaac
    13381 agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac ctggtacata
    13441 agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa catcataagg
    13501 attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt ctctgggatg
    13561 agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg caaatcttcg
    13621 catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt tgacaaaaaa
    13681 aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg
    13741 tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa aaataattgg
    13801 aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg
    13861 attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg
    13921 cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga
    13981 cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg tgaattagca
    14041 cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc tggctgtaat
    14101 gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac gttcgcttct
    14161 tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca gctggtcctt
    14221 ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca ggagtcagtg
    14281 cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca
    14341 aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc tccaacagga
    14401 taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga gtcggaagct
    14461 taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta aaatggttac
    14521 ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat cccacgactg
    14581 gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc ttattcttga
    14641 tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat cgtacctaat
    14701 tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat aagcgtcact
    14761 tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat acatacacgc
    14821 ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat ctttcgtggc
    14881 cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg agtgatatat
    14941 gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat caattcagaa
    15001 accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc agagggatta
    15061 agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc atgccataaa
    15121 gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt tttcagcact
    15181 tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc tgctgtagaa
    15241 aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa tgcagctgcc
    15301 tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc tgcttttaga
    15361 tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc tgcttgcaac
    15421 cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt cagtgcgtcg
    15481 taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct caactcgact
    15541 ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc tgcctcagct
    15601 gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa ttcttttcct
    15661 tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt atgtatcact
    15721 gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta gtcttccaaa
    15781 ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac attgccttca
    15841 tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc gacatcttga
    15901 aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt gcaagcgtaa
    15961 cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag tttcatttca
    16021 tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg agtgatccta
    16081 ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct tttcaataaa
    16141 tttagaacac tctcattcta ccaaattata accattttgc cacaaataag tccacaaata
    16201 acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta atcgttattt
    16261 gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc tttttggctg
    16321 ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc gccgtcacga
    16381 cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt tttctgcatt
    16441 ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc ttttgctgtc
    16501 gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa tttatctgct
    16561 gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg ttcaactgca
    16621 gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt
    16681 tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt aacgaaagtc
    16741 acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac gtagctttct
    16801 ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc taatccacgt
    16861 gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga acgcaatacg
    16921 ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta accgcgtgct
    16981 tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac gattgttaat
    17041 tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta ttcttttgcg
    17101 cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat cttcacatga
    17161 tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat cgttgctgag
    17221 aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat atcttccaag
    17281 aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac ggttccaagt
    17341 ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac aacgatatgt
    17401 ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta aacggcttgt
    17461 acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg gatagctaat
    17521 tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc gattttttct
    17581 aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg tccaataacg
    17641 tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa
    17701 ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt aaatttcaaa
    17761 tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc cgtcgatttg
    17821 ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta tccattctta
    17881 gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa aagccaaacg
    17941 ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt ttctatggca
    18001 aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata gtttttcgtt
    18061 ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac ttcaaataag
    18121 cccatgctgt tgctgccttt tagcataata ctatctgttg cttcaagatt ttgtttgata
    18181 gcatcaatca atttattctt ttctttttcc aaatagaaca caggtagttg ggggtatttt
    18241 tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat catggcgaat
    18301 ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc tttccctaat
    18361 tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt tttaaaagta
    18421 tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa aatagctgca
    18481 ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg ggcgagccct
    18541 tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc aatcaaagca
    18601 ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc tactacaaaa
    18661 tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt tccaataccg
    18721 aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg
    18781 ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt ggctttagcg
    18841 attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt gattgctgca
    18901 gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc catgcccatt
    18961 tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata
    19021 ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa gacagacgcg
    19081 gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct
    19141 actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc tgctacagga
    19201 ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga agcagatgct
    19261 ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc aagaggaacg
    19321 aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat ctttgtcagt
    19381 gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt cattgagatc
    19441 tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg catgactctc
    19501 acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg ttacgcaatc
    19561 actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat tgacgctgat
    19621 ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat ttcaagccca
    19681 tattt
//
LOCUS       DAYLZO010000053        19508 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.054, whole genome shotgun sequence.
ACCESSION   DAYLZO010000053 DAYLZO010000000
VERSION     DAYLZO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.054"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACGVJG_001660"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACGVJG_001660"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFJ6557602.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACGVJG_001661"
     CDS             complement(1174..1869)
                     /locus_tag="ACGVJG_001661"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFJ6557603.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACGVJG_001662"
     CDS             complement(1882..2598)
                     /locus_tag="ACGVJG_001662"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFJ6557604.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="ACGVJG_001663"
     CDS             2928..3533
                     /locus_tag="ACGVJG_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFJ6557605.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="ACGVJG_001664"
     CDS             3858..4181
                     /locus_tag="ACGVJG_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFJ6557606.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="ACGVJG_001665"
     CDS             4363..5733
                     /locus_tag="ACGVJG_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFJ6557607.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="ACGVJG_001666"
     CDS             complement(5863..7164)
                     /locus_tag="ACGVJG_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFJ6557608.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="ACGVJG_001667"
     CDS             complement(7394..9385)
                     /locus_tag="ACGVJG_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFJ6557609.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="ACGVJG_001668"
     CDS             complement(9529..9795)
                     /locus_tag="ACGVJG_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFJ6557610.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="ACGVJG_001669"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="ACGVJG_001669"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFJ6557611.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="ACGVJG_001670"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="ACGVJG_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFJ6557612.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /gene="def"
                     /locus_tag="ACGVJG_001671"
     CDS             13139..13702
                     /gene="def"
                     /locus_tag="ACGVJG_001671"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFJ6557613.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="ACGVJG_001672"
     CDS             complement(13955..14476)
                     /locus_tag="ACGVJG_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFJ6557614.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="ACGVJG_001673"
     CDS             complement(14479..15321)
                     /locus_tag="ACGVJG_001673"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFJ6557615.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="ACGVJG_001674"
     CDS             complement(15314..16039)
                     /locus_tag="ACGVJG_001674"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFJ6557616.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="ACGVJG_001675"
     CDS             16215..16511
                     /locus_tag="ACGVJG_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557617.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16767..16941
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17079..18671
                     /locus_tag="ACGVJG_001676"
     CDS             17079..18671
                     /locus_tag="ACGVJG_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFJ6557618.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..19424
                     /locus_tag="ACGVJG_001677"
     CDS             18684..19424
                     /locus_tag="ACGVJG_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557619.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5581 a         4002 c         3702 g         6223 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    19441 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
    19501 tttctgaa
//
LOCUS       DAYLZO010000054        19422 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.055, whole genome shotgun sequence.
ACCESSION   DAYLZO010000054 DAYLZO010000000
VERSION     DAYLZO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.055"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1104
                     /locus_tag="ACGVJG_001678"
     CDS             <1..1104
                     /locus_tag="ACGVJG_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HFJ6557620.1"
                     /translation="DDVWFSGLIGLGISLLALVALYQYLAEDTTKLGSEYEKDRFDRL
                     LETYGGTATSHQLRLSGYSYFYYQEDDQDQVVFGYQIKGNKCFVLGDPVGNPKMMRAA
                     TNQFMKQADCLGYQLAFYKISESYVVMLHELGFHFTKVGEAGIVDLATEDLSRLSQQL
                     EYQRLMKEGYHFTWYDQLPEELHQAVNEVSQKWLQGAREKYFSVGRFDKSYVLSSGVG
                     IVRKDQKVVGFITAQPMTKKQAGYDLLRVLPKESEELADYLLANLFIVYEKMGYSEAN
                     LGLAPLANVGETDFSFFQEKVMHIVYNYGNFFYSFQSAYEKKLKYVDHWEGRYFAYRK
                     GSNFGFATMQLLSLIGKEKGNVPSLAEEVLTEL"
     gene            1258..2109
                     /gene="ispE"
                     /locus_tag="ACGVJG_001679"
     CDS             1258..2109
                     /gene="ispE"
                     /locus_tag="ACGVJG_001679"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFJ6557621.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            2354..3292
                     /locus_tag="ACGVJG_001680"
     CDS             2354..3292
                     /locus_tag="ACGVJG_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFJ6557622.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            3315..3995
                     /locus_tag="ACGVJG_001681"
     CDS             3315..3995
                     /locus_tag="ACGVJG_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557623.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            3968..4792
                     /locus_tag="ACGVJG_001682"
     CDS             3968..4792
                     /locus_tag="ACGVJG_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFJ6557624.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            4959..5777
                     /gene="purR"
                     /locus_tag="ACGVJG_001683"
     CDS             4959..5777
                     /gene="purR"
                     /locus_tag="ACGVJG_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFJ6557625.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            6280..7653
                     /gene="glmU"
                     /locus_tag="ACGVJG_001684"
     CDS             6280..7653
                     /gene="glmU"
                     /locus_tag="ACGVJG_001684"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFJ6557626.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            7747..8718
                     /locus_tag="ACGVJG_001685"
     CDS             7747..8718
                     /locus_tag="ACGVJG_001685"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFJ6557627.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      8724..8826
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8892..9461
                     /gene="thiT"
                     /locus_tag="ACGVJG_001686"
     CDS             8892..9461
                     /gene="thiT"
                     /locus_tag="ACGVJG_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFJ6557628.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(9542..10315)
                     /locus_tag="ACGVJG_001687"
     CDS             complement(9542..10315)
                     /locus_tag="ACGVJG_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557629.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            10582..12282
                     /locus_tag="ACGVJG_001688"
     CDS             10582..12282
                     /locus_tag="ACGVJG_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFJ6557630.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            12252..12470
                     /locus_tag="ACGVJG_001689"
     CDS             12252..12470
                     /locus_tag="ACGVJG_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFJ6557631.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            12490..13380
                     /locus_tag="ACGVJG_001690"
     CDS             12490..13380
                     /locus_tag="ACGVJG_001690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFJ6557632.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            13398..13682
                     /locus_tag="ACGVJG_001691"
     CDS             13398..13682
                     /locus_tag="ACGVJG_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557633.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            14169..14783
                     /gene="gmk"
                     /locus_tag="ACGVJG_001692"
     CDS             14169..14783
                     /gene="gmk"
                     /locus_tag="ACGVJG_001692"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFJ6557634.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            14788..15093
                     /gene="rpoZ"
                     /locus_tag="ACGVJG_001693"
     CDS             14788..15093
                     /gene="rpoZ"
                     /locus_tag="ACGVJG_001693"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFJ6557635.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            15346..17754
                     /gene="priA"
                     /locus_tag="ACGVJG_001694"
     CDS             15346..17754
                     /gene="priA"
                     /locus_tag="ACGVJG_001694"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFJ6557636.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            17769..18260
                     /gene="def"
                     /locus_tag="ACGVJG_001695"
     CDS             17769..18260
                     /gene="def"
                     /locus_tag="ACGVJG_001695"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFJ6557637.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            18253..19191
                     /gene="fmt"
                     /locus_tag="ACGVJG_001696"
     CDS             18253..19191
                     /gene="fmt"
                     /locus_tag="ACGVJG_001696"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFJ6557638.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            19191..>19422
                     /locus_tag="ACGVJG_001697"
     CDS             19191..>19422
                     /locus_tag="ACGVJG_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFJ6557639.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAK"
BASE COUNT         6484 a         3359 c         4216 g         5363 t
ORIGIN      
        1 gatgatgtgt ggttctcagg gcttattggt ttgggcatct cattgctagc tttagtggcg
       61 ctttatcaat atttagctga agatactacg aagcttggca gtgagtacga aaaagaccgt
      121 tttgaccgtt tattggaaac atacggaggc acagctacaa gccatcagct tcgcttgtca
      181 ggttacagtt atttttatta ccaagaagat gaccaagatc aagtcgtttt tggttatcag
      241 atcaaaggaa acaaatgctt tgttttagga gatccagttg gaaatcctaa aatgatgcgg
      301 gcagcaacga atcagtttat gaaacaagca gattgtttag gctatcagct tgcgttttat
      361 aagatatcag aaagctacgt agtgatgctt catgaattgg gttttcactt cacgaaagta
      421 ggtgaagcag ggattgttga tctagcaaca gaagacttgt ctcggctgag tcagcaactt
      481 gaatatcaac gtttgatgaa agaaggatac cattttactt ggtacgatca actgccagaa
      541 gaattacatc aagctgtaaa tgaagtttca caaaaatggc ttcaaggtgc acgagaaaaa
      601 tacttctctg tgggacgatt tgataaatca tatgtattat ctagcggtgt cggtattgtc
      661 cgtaaagatc aaaaagtcgt tggcttcatc accgctcagc cgatgacgaa gaagcaggcg
      721 ggatatgatt tgttgcgggt cttacctaaa gaatctgagg aattagctga ctatctgtta
      781 gccaatctgt ttattgtata cgagaaaatg ggctactcgg aagccaatct aggcttagct
      841 ccattagcta atgttggaga aacagatttt tctttctttc aagaaaaagt catgcatatc
      901 gtatataatt atggtaattt tttctattcc ttccagtctg cttatgaaaa gaaattaaaa
      961 tatgtcgatc attgggaagg acgttacttt gcttatagga aaggaagtaa tttcggattt
     1021 gctaccatgc agctactcag tctgatagga aaagaaaaag gaaatgttcc gagtttggca
     1081 gaagaagtgc tcacagaatt ataagaatca ctagaacttg tttggattat gaagagaaaa
     1141 ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact aggcagacat aagctatact
     1201 tttatttttc cctatgatag aatacttgcg acttagactt ttcgaatggg tgagaggatg
     1261 gaattgattg aaaaagcgcc agccaagata aatttaggac tggatgtatt atatagacgg
     1321 caagatgggt atcatgagtt ggaaatggtg atgtcgagcg tagacttggc agatcatcta
     1381 ttttttgaag aaataacaga agacaaaatc attgtagaaa caaataaagc atttttaccg
     1441 gtagatgaac ggaacaatgt ttatcaagct gctttattgc taaaaaataa atacaatatc
     1501 aaaaccggtg tcaagatctt tgttaagaag aatattccag tagcagctgg attaggcgga
     1561 ggaagtacag actgtgcagc tgctctaaga gggatcaacc aattatggaa tctgggctgt
     1621 tcgttagaag aactggcaga aatcggtttg gaagtcggta cagatgttcc ttactgtgtc
     1681 tacggaagta cggcatttgt cggtggcaaa ggggaagaaa tccaagtatt accttctatg
     1741 ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg tctctacacg tacagtgttt
     1801 agagaagtag atacgcaagc catccaacat caagacatcc acggacttgc tgaagccatc
     1861 cgagagcaag attatcagca gatgatcaaa aaaatgggaa acagtttaga agatataacg
     1921 ggaaagcggc atccagtcat ccatcaaatc aaagagcgta tgatcaagta cggagcagat
     1981 gctgcattga tgagtggaag cggaccaacc gtttttgcct tgtgtcaaca ttattcaaga
     2041 gcgcagagag tatataatgg gttaaaaggt ttttgtgagg aagtttattt agtacgaacc
     2101 ctgcaataaa atataagaag taaaataggt gatgaaaaac tgaggaatcc taaaatggta
     2161 ttcttcagtt ttttattgta gtgaaatggc tagatatgag cagcaactca tccattttac
     2221 gatttaatca agaatataca tgcttaaatc agaaattgat ggatttctat tgactgtgtt
     2281 tttttatttt gatacattag ttcacgttaa taaatcgtaa aattccgatt tgttcattga
     2341 aaggagcata taaatgaaaa aatatctttt gacagctacg gtaatgatag gtgctttact
     2401 gtttgcagca tgtggaaata cgaacaagga agcagacaaa aaagaggatt tgacgatcgt
     2461 tacgactttt tatccgatgt atgattttac aaaagagatc gtaggggatg aaggaaacgt
     2521 gaaacttttg atccctgctg gaacggagcc gcatgatttc gaaccaagtg caaaagaaag
     2581 agcagaaatt tccgatgcag atgttttcgt gtataatagt tcagacatgg aattttttgt
     2641 ggattcgtta aaggattcag tggatagcaa acagaccttg atgatcgaag cagctaaagg
     2701 aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa gaatcagaag aagggcatgg
     2761 tcattcacat gaatacgatc cacatgtttg gctcgatccg gtattagcta taaaagaagt
     2821 cagaacgatt gctgaagaat taggcgaaaa atatccagat aaaaaggaaa cttttacgaa
     2881 gaatgccgat gcttatatca aaaagcttga agcactggat caaaaatact ccgatgagct
     2941 aaaagatgca acaaaccgca catttgtcac tcagcatgct gcttttgctt atttagcgaa
     3001 tcaatacgac ttaaaacaag tagcgatttc gggcgtttca ccagatcagg aacccacacc
     3061 aagtcgctta gctgagctaa aagaatttgt gaagaaaaac aacatcaaag tgatttattt
     3121 tgaagaaaat gcctcttcga aggtagctga gacattgtcg aatgaaacag gagtgaagct
     3181 agaagtgttg aatcctttag aaagcttgac taatgaacaa atcaaagcag gcgaaaatta
     3241 tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa agtatcaatt aagttttaga
     3301 atggaggttg taaggtgcgt tatatcgaaa tatctgattt gaccttttac tatgatgatg
     3361 aacctgtact agaagatgtc tcttatcatg tagatgcggg tgaatttgtc attttaactg
     3421 gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc gttaggatta ctgaaacctt
     3481 caaaaggaat gataaagatt gctgagaaaa atcaagaagg aaaaaaatta agtatcggtt
     3541 atattcctca acaagttgca tctttcaatg caggttttcc tagtacggtg atcgaattag
     3601 tccgttcagg aagatatcca agaggccgct ggttcaagcg tctctctgaa aaagatcatc
     3661 aacatgtaca gcgtgcttta gaagctgtag ggatgtggga tatgcgaaat cggcgaatag
     3721 gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc gcgtattttt gctactgacc
     3781 ctgacttatt tgttcttgat gaaccaacaa caggtatgga tgaagaatcc agaaatgaat
     3841 tttatcgtct tctccgacat agtgcgcatg tccatgggaa atcagttcta atgatcaccc
     3901 atgatcatga agatatcaaa cagtatgcgg acagacagat ccgactggtg agaaaggagg
     3961 attctcaatg gcgttgcttt catatgaatt catgagaaga gcgtttcttg ccgctatctt
     4021 cattgccgga atcgccccga tgctaggtgt gtttttagtg atcagaagac aatcgctgat
     4081 ggctgatacg ctttctcatg tctctttagc aggggtagcg ttaggattct ttttgaacct
     4141 taatcctaca cttacgacca tgctagtcgt tgtattagca gctgtgatat tggagtatct
     4201 gcgaacgatc taccgaacct attcagaaat ctccattgcc attttaatgg ctggtggttt
     4261 ggcgctagcg ttagtattga tgaatttgag cggagggaat tcagcaacaa gtatccaatc
     4321 ctatctgttt ggttcgatcg tgaccattac atgggagcaa gtcatctttt taggaatctt
     4381 attcgttgcc atcggtatat tgttcttttt attcaaacgg ccgatgtatg ttctgacttt
     4441 tgatgaagat accgcccacg tggatgggct acccgttcat ttgatgtcga tgatgttcaa
     4501 tgtgattact ggtgtggcta ttgctgtgat gatcccgatt gccggtgcac ttttgatatc
     4561 cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta ggcaaaagct tcaatgtcgt
     4621 gattgctatc agtgtttgtg taggatttct agggatgctg tcaggactaa ctagctcgtt
     4681 ttatctagat acacctccag gagcaacaat cacattagtc tttattgctt tgttcctgat
     4741 tgtaaatatc atcaaacgaa tattggttgc cttcagaaga aaaaataact aaattataaa
     4801 gaaaagaagc cttataaaaa cgaaaaattt tttataggct tctttttatt ttaataaata
     4861 ccgaactatt ttcttatttt ttcataaaat gctcgaattt ttgaagaaaa acttatataa
     4921 tgtaaaacga taaaatttct taaaaggagt tggaataggt gaaagtacgt cgcagtgaac
     4981 gtctagttga catgacacag tatttattga atcacccgca tgaattgatt tccttaacaa
     5041 gctttgccga acgttatgaa tctgctaaat cttctatcag tgaagatttg ggaatcatca
     5101 agaaaacatt taaagaacga gggtatggaa ccttggaaac aatcccgggt gctgcaggtg
     5161 gtgtattgtt catccctgaa atttcatatg aagaggcgaa aaaatatatt gaatctctag
     5221 cagaacgtct atcagaacaa gaccgtctgc ttccaggagg atatgtttat ctctctgatt
     5281 tgttaggaga acctgaactg ctgcgtcaag ttggaagaat cattgcttct aaataccttg
     5341 gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg cgtacctatt gcccaagctg
     5401 tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg ggactcaaaa ataacagaag
     5461 gatcaacagt aagtgtcaac tatgtgtctg gatcttctga aagaattgaa aagatggaac
     5521 tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt agtggatgat ttcatgaaag
     5581 gcggcggaac agtcaacgga atgaagagct tgattgaaga attcgaatca gaacttgttg
     5641 gcatcactgt atttgccgaa tcaaaattca acggtcatcg cgccatcgat gattatacgt
     5701 cactgcttta tgtcaaagat gtagatactc aaacgaaaaa tattacggtt gtgccaggaa
     5761 attatttttc agaataagag gttgtgaaaa agccgcttag tttcgagtag taagaaaaat
     5821 tttgtgaaaa tggctttcca cattttttca taaatttggc ttaataccga gaaactggct
     5881 tttgaacacc gtttatctag aggttgtgaa atcagcgtcc tgtcctgagt attagctcaa
     5941 taaacggaaa aattgctcct catattttgc cgtttattga gctaataccg aaggtcagct
     6001 gattgaaaaa cgtttaatga agaggttgta agcctaccgt ttaactctaa gtcacaagaa
     6061 acgatttcaa aaaacagctt ttcatgtttt atgagtagtg tgacttgtga ccgaggagtt
     6121 ggcaggagag caccggttac caaatagata ggcccgttat ggtctatcta tttttttatc
     6181 cagaaaacgc tgggataaaa gaaaatcact tgttttgttg aaaaaatggg tatactggga
     6241 taaactgaaa tcagcaaact atatttagga gtgacgcagt tggacgcacg ttatgcaatt
     6301 attttggcgg cagggaaagg tacccgcatg aagtcaaaat tatataaagt tttacatccc
     6361 gtttctggta aacctatggt agaacatatc attaatcggg tcagtgaaac gaaaccagat
     6421 gaagtgatta cgatcgttgg acacggagca gaacaagtta aagcacaact aggagaacgc
     6481 agtaaatatg cgttacaagc agaacaattg ggaacaggac atgctgtttt gcaagctgct
     6541 tctttcttgg aaggaaaaaa aggaacaacg ttagttatca gtggcgatac accattattg
     6601 actactgaaa cgctgaacaa tctttttgaa tatcaccaag gaaaaaatgc aagtgcaacg
     6661 atcttgacgg cacaagcaga aaacccaaca ggatatggac gaatcatccg tgaccatatt
     6721 gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac cagaagaagc tcttgtacaa
     6781 gaaatcaata caggaactta ctgttttgac aacgaagctt tgttcgatgc gttaagcaaa
     6841 gttggaacaa acaatgctca aggtgaatac tatctaacag atatcatcga aatcttaaaa
     6901 gaagaaggcc atacagttgc tgcttatcaa acggatgatt ttgaagaatc aatgggagtc
     6961 aatgatcgga tcgcgttagc taaagcaaat gagatcatga gaaagcgaat taaccagatg
     7021 catatggtca atggcgtaag ctttgtcgat tcagcaacga catacatcga tgcaggcgta
     7081 gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa tccaaggaaa tacagtcatt
     7141 ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag acagtaggat tgaggatcac
     7201 gtcgtcattg aaaactcagt cattgaaagc agccatgtca agaaacatgc agatgtggga
     7261 ccttatgcac atttacgacc aaaagctgaa attggcgaaa acgtccatat cggaaacttt
     7321 gtggaagtga aaaatgctca aatcggcaaa ggaactaaag tcggtcactt gacttatgtt
     7381 ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg gtgttgtctt cgtcaactat
     7441 gatggaaaga acaaacatca tacgactgtc ggcgaccaca gctttattgg ttctagcacg
     7501 aatatcatcg gacctgtcga ggtagctaaa aacagttcga ttgctgccgg gtcaacaatt
     7561 acagacaata ttccagaata tgcgctggca attgcgagag caagacaagt caataaagaa
     7621 ggatatgcaa aaaaactccc ttatctaaac taagactttt gcaaaatcac ttgatttatt
     7681 taaaaaatat gactactatt tattaggtag aattctagag ttgtgaaaaa gaaagtggag
     7741 gtccccatgt caaaacatta ctttgaccca agattaaaaa tttttgcgtt aaattccaat
     7801 cgcccattag cagagaaaat tgcagcagca gtcggcgtcg agttaggaaa atcatctgtt
     7861 actcaattta gtgatggaga aattcaagta aacattgaag aaagtattcg tggatcgcat
     7921 gtctatgtta ttcaatcaac gagcagccca gtcaatgata acttgatgga gttgctgatc
     7981 atgattgacg cactgaaacg agcaagtgca aaaacaatca acgtagtcat gccttattat
     8041 ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac caatcacagc gaaattagta
     8101 gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga ctttggacct tcatgcagtc
     8161 caaatccaag gtttctttga tattccagtc gatcatttga tgggtgcgcc acttattgcc
     8221 aattatttct tagaaaaagg aatcaaaggc gatgatgttg tcgttgtttc accagaccat
     8281 ggtggtgtca cacgggcacg taaattagcg gaatttttga aatctccgat tgcgatcata
     8341 gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga atatcattgg acaagtcaaa
     8401 ggcaaaactt gtgtactgat cgatgatatg atcgatacag caggtacgat cacattggct
     8461 gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg cttcatgtac acatccagta
     8521 ttatccggcc ctgctttaca aagaatagaa gattcagcta tcgagcgtct agttgttaca
     8581 gactcgatct atcttccaga agatcgtaaa atcgacaaaa tcgatgaagt aagtgttggc
     8641 ggactaatgg gagatgcaat caaacgtatc catgaaaaca aaccagttag tccattgttt
     8701 gaaacaaaaa ataaataata aataaactca caaaggggag tccaattggg ctgagattga
     8761 atctatttct aaacccttcg tacctgtatc ggttatgcga gcgtaggaat tgtgaataaa
     8821 cagctgtttg actattttcg aaagctgctt cctcctttgt gactttttta ttcaaaggag
     8881 gaagtttttt tatgggaaaa aatagagttt gggtagaagg aacgattgtt gcagcgttag
     8941 cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag tttttctatt tcattagggc
     9001 agattccatt gactcttttt gcgttaagaa gagggacaaa ggcagggatg ctggcaggtt
     9061 tcatttgggg catcctacat tttccgttgg gacaagttta ttatttaagt gtcgttcaag
     9121 tgttgattga gtacccgatt gcctatactt ttgcaggcgc agcagggctc atggcttcaa
     9181 atgtccaaaa aggctatgta gacgataata cgaaacaaat tagaaaaagc atcgtgtttg
     9241 gtgcattgat tggcgcactt tccagatatt tttggcattt tattgcagga gtggttttct
     9301 ggggagcgta tgcactatgg gggatgaatc cttggctctt ttctttcgta atgaatggtg
     9361 ccagcgggtt gagtacagct attgtaacga tcatcgtttt attagggatc caacaaacga
     9421 ctccttcgat ttttgtgcca gataaacgac aggagctgta aataaaaaag aagcaagaaa
     9481 cttgagacag aatcatgtcc tagtttcttg tttccttttt attttttgat aagaaataga
     9541 tctacttcaa tggcagataa gaaagatcgg catcttctac caattgccag tgtcgatcgg
     9601 tgacagagta gattaaagta gcgatgattt cctcttctcg ataccgggtg ccttttaatt
     9661 tccaaatata atcttcaatc acatagattg ggatacgata tttaccatcg atcaaaccaa
     9721 cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt acgcatttct ttcaataatt
     9781 ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa agattttttt acattgatct
     9841 tatctgacaa gatcgaactt aatttactag caaaaatcct ataaaattca tctaaatagc
     9901 gataataaat ccgattcagc tgatcattaa gatcgaagta tacaaaatta ttctgtagtt
     9961 tataaaaaaa cggcgaatga agatgtgttt tcatatgacc aaaatataat aattctgaaa
    10021 tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt
    10081 catcgaatcc acgcctgtta cgagaagagg aaaagaaacg ctgaatattt tctgtaccac
    10141 ggattacttt cagccctgta tgggattcat attctccagc agaagcagat ggatcaagca
    10201 agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt
    10261 ttgttaaaac agcattactt gtggtatcaa tatgtacata aactgactca gtcatcatgt
    10321 tcctcctctt atttcctctt aatctctttt attttacaac atttgcatta aaaaagcttg
    10381 tttcttttat tttaaaattc cattacaaaa tcacgttttc attgaaaaca ctctcatttt
    10441 caattaattt ttgtgatttt tgtatttttt tatttaaagg aggttttatg aattgttttc
    10501 ttatggaatc tacaaagtcg aatacgagac tcatgacctt tttatgacgt actggtatac
    10561 ttgaagaaaa aggagagcgc gatggagata aaaaaagttt acttttcaca gaaaatgtcg
    10621 ctgtattcgc ctatccagca agcaaaacaa ccccttccat tttttcaaag tcatcaagaa
    10681 agtagagacg ctttttggcg ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa
    10741 aaatataccg tctattttac tattgctaaa agtagaggag atgttatttt taataatctg
    10801 ttgattgaag gtgaaacttt tgaatggaaa aaattttttc ggtatatgga agcttgcgct
    10861 cgttttttta ttaaagaaaa ctgttgtttt ttatttcaat ctcctctatc agaagaatgg
    10921 ttgcgtattt ttcataaaaa cggctatcaa ggaaccacac aattaaataa aaagctggtc
    10981 tatcatacag cacttgtttt aggtggagga ggagcgcacg gtgcttatca gattggtgtg
    11041 tggcaggcgc tgaaggaaca tgatatcaac tttgagatta tcacaggtac ctcggtaggt
    11101 gcgttgaacg gcgcattgat tttacaagga gatatgaaaa aagctgtgaa tttatggaaa
    11161 aagctgtcga caagacaagt gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt
    11221 gaacgcttct atcgagaaag acgccaaatg acgaaaacag cactaattga aggcggggtt
    11281 tcttctgctc cattggaaaa gatggtcaaa gacaatttgg atttgtcctt actcaaacat
    11341 aatcccaaaa tacgtttata tacggtgtct actaaactgc cagaactagc ggaagtagtg
    11401 actgatatcc aacagacaaa aacagaagaa ataccagatt ggatactcgc ctctgcttca
    11461 ttttatcccg ctatggctta tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat
    11521 cgcaacaaaa tacctattga tattgcgatt aacaaaggag caacagaggc atttgtcgta
    11581 gatgtgcaag ggccaggccc agcgaaaaaa attcgtatgc cagacatttt tatccattgg
    11641 aaatgtcaaa cattgtggac actaggcagc tttctgcttt ttgatagcca aaggaaccaa
    11701 ttaaatttac aattaggcta tctggaaatg aaaaaacgat taggctatta ctatgggaac
    11761 tggtatacat ttcattctgt taaacctgca agaacatact ggcgaggatt tttgtcttac
    11821 ttaacaaaag aaattcagtt agaattatct tttttcaaaa gcataaaatt ttggcaaaaa
    11881 ctgcgtaatt tatataagaa cagggtcgta ccagagacat gcggattggc gatgctagaa
    11941 cttttagcaa aaaaacattt tttactgccc aatgaaatct atcaagtaga catgatgatt
    12001 caaatgatct gccaacaaga tataaaaagt atgccggatg atttattggc acaaattggt
    12061 caattaagta ctgaagaatg gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag
    12121 cgcacgaaaa ctgtttattt tgattatctg ctacaaggaa aacgcaagga tcgtttgcag
    12181 catgaattgc ttaagcaacc agttgacaca ttactgatcc tatatttata ttatctcaag
    12241 gaggaacaac catggcacaa gaattttcat atgaaatcgt agaagaaatc gctatattgt
    12301 cagaaaacaa caaggggtgg cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc
    12361 caaaattcga tcttcgagat tgggcgccag accatgaaaa aatggggaaa ggcctgacat
    12421 tgacgaacga agagtttgaa caactgcaaa aagcaataga aaatatgtaa tgaggaagga
    12481 ccctgtcgaa tgaaaagtat gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat
    12541 cagttagata ttgaaataaa aagtgtcaac cagcgttttc tagatgttca aatacgaagt
    12601 cctaaaatcc tgaactttgt tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc
    12661 cgcggtcgaa tcgaagtatt tgtgaatcta gcttatattg gcaaaaacga aaaacagatc
    12721 gtcatcaact gggaattgat tgattcactt atcactcaat tgacagacgg aattcaacaa
    12781 cgatacggta gtcaaaatca agtgtcaatt agtcggatgg cggaacgctt cgtgctggac
    12841 gaaaacttcg taacggtaga agaaaaaccg gaagagagca tggaagaatt ggcacagtta
    12901 gctctagata cattgaaaag cgcacttgat tctattgagg cgagtcgaga aaaagaaggt
    12961 caagctttag ctttagtgtt gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa
    13021 ctcgatagtt ttgtagaaat ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg
    13081 gaggaatttc ttggagcgaa agtcgatgag caacggttgt tgacggagct agccatcttg
    13141 ttggaacgcg gagatattca cgaggaactg gatcgtctgg cgatccatgt acaaaaacta
    13201 gatgaactta tcgaagcaca atgtcctgta ggtcgggaat tagatttcct gatccaagaa
    13261 atgaatcggg aaatcaacac gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa
    13321 gtcgtccagt ctaagacgat cttagaaaaa atcagagaac aagtacaaaa tattgaatga
    13381 cgaaagaggt agagataatg aaatattatg acgtaacgtt ccacgaatta agtggaaaaa
    13441 ctgtggttaa acgggatatc ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct
    13501 gtgcagacta taatgagaac gaactgttta ttttgatcaa cgacggtgct tacgtgacga
    13561 tgaatcggaa atttattgtt cgaattgaca cagaagaggt agaagatccg acagaaaaag
    13621 cacgttctcg aaaagatgag atcatgggtg tagtgaatac tttgtccaat atgggctttt
    13681 agcaaggatg ctgaacaaag aaatacaata aataaacaga attggatttc ttcgcttagc
    13741 ttctttgctc taggaataca tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt
    13801 attttgaata agtgatgttc aaaaaccatt ttctcggtat taagccaaat ttatgcaaaa
    13861 atgtgagaaa ctattttcac aaaatttttc ttcatactcg aaactaatcg gcttcttcac
    13921 gacctcttat acacggcgtt caaaagcttg catctgcgat aataacccca accaaacgaa
    13981 aaacatgaga agctgtttat gtttggctgt tcttattact tgatgcagga cagctttttt
    14041 acgacctctt ggtacagggt agctttttta ccaatttcta gctataatta aagataacct
    14101 tgaaagttgc agaaaaaaag tgcaaaataa acgataagta caaaaagact agttgaaagg
    14161 acgtcgtcat gtcagagcgt ggattattaa tcgtgttgtc tggtccatcc ggtgtaggaa
    14221 aaggcacagt acgaaaagca atttttgaga gtgaggataa tgattttcag tattcgatct
    14281 caatgactac ccgtaaaatg cgtgaagggg aggtcgaagg cgttgattat ttcttccgaa
    14341 gcaaagaaga gtttgaatca atgatcgaag ctggagagat gctggaatac gcagaatatg
    14401 tcgggaatta ttatggaact cctttgtcat atgtcaacaa gacattggat gaaggaaaag
    14461 atgttttctt ggagattgaa gtccaaggag caaaacaagt caaagaaaaa gtgcccgatg
    14521 gtgtattcat ctttttgact ccgccagatt tagcggaatt aaaatcacgg atcgtaggcc
    14581 gaggaacaga tgcagatgaa gtaatcgaaa aacgaatgaa agttgcaaaa gaagaaatcg
    14641 aaatgatggc tctttacgac tatgctgttg taaatgatga agtgccgcta gcagttgaac
    14701 gaatcaaaaa cattattgcc agtgaacatt ttcgagtaga tcgcgtaatc ggtaaatata
    14761 ttaaaatgtt aaaggagatg tagtcttatg atgttaaaac cttcaattga ctcactatta
    14821 gatcgtgtaa attcaaaata ttctctagtg attttagcaa gtaaacgcgc gcatgaattg
    14881 gatgcaagtg cacagcctac tctagattct tttgattcag taaaaagcgt cggtcaagct
    14941 ttagaagaaa tcgatgcagg aaatgtagtc aatgatccac atcctgaatt aaaacgtgaa
    15001 cgtttgaaga tggaagaaga ggaacgtcaa gcacaaaaag agcgtgaaca acacgaattg
    15061 gaatcacgta tccgtgaaga gcaaaaaatg taataaagaa aaatagagcg aacatctaaa
    15121 aagaaaagca aacccagctg tttccttttc aaacattcgc cacttattgt ctggtagaaa
    15181 aggctgtgtc tgaacttttt gacacagttc ttttttttct tatctgctgt taattttttg
    15241 ttaggatttg gcagaagata agacaaatcc atcgatctct agtaaaatag gcaaggaact
    15301 ttttgtcgct tgataagaaa agacaaaaaa aggaggaaac gaaccatgac attaacagct
    15361 gaagtcattg tggacgtacc tactatgcag accgatcagc cattcactta tttggttccc
    15421 tctgaaatgg aaacagcttt gcaagtcggg atgcgagtag aagtcccttt tggtaatggc
    15481 aatcgtcatg tccaaggatt tgttatggct atccaaaaat cagaagaatc agctaatcca
    15541 tctttgaagg cagttattcg tttgctagac cttgctccgg tggtcaatga agaattactt
    15601 tcattagctg attatatgaa aaaaattact tacgcattca aaatcacttg tctacaaacg
    15661 atgcttccga gtgtgatgaa agcagaatat gataaattga tctatccttt ggcagacaca
    15721 ccagaagttc aggtattgtt tgatcagcga gaagtgattt cctggaagga agcagaagaa
    15781 gaaggcagcc tttctcaatt gattcgctgg cgccaagagc aattagtaga tatcaaatat
    15841 gaagtccata cacgaaataa agtaaaaacg attcgtttgg tccgttcttt gttaactgaa
    15901 aagcaaatcg aagaagaatg ggcaaaatta cggcaaaatg ccaagaaaca aaaagaactt
    15961 cttttgtgtt tatctgaact gagccaagaa gaaccaattg cttatttcaa aaataaagga
    16021 atcagtactg ctgtcttgaa tcaaggaaaa gaaaagggct ggctggagtt tatagaatca
    16081 gaacgttatc gcgaccctta taaagaccga gtatttgacc aaacgacggc attggaactg
    16141 aatgcagaac aaaaaaatgc tgtcgaacaa atcatcacag caggtcagca gcaaaaagat
    16201 caggtctttt tgctggaggg aatcactggc agcgggaaaa cagaagtata tcttcaggca
    16261 atcgctgatg ttttgtcaga aaataaaacg gctattatgt tagtgcctga aatcgcattg
    16321 actccgcaaa tggtcgagcg attcaaagga cgcttcggag aatcagttgc ggtcttacat
    16381 agcggacttt cccaaggtga aaaatatgat gaatggcgca agattgagcg ggaagaagcg
    16441 caagtagtag taggcgcacg atctgctatt tttgccccgc tgaaaaatat cgggctgatc
    16501 attatcgatg aagaacatga atcaagttac aaacaagatg agacaccgcg ttatcatgcg
    16561 agagatctag caatttggcg cagtaaatat catcattgcc caatagttct aggaagcgcg
    16621 acgccctctt tagaatcaag agcacgagct caaaaaggcg tttatcagtt attgcagtta
    16681 aaccaccgag ccaaagcagc tgcacagctt ccagctattg agatcgtaga tatgcgtgaa
    16741 gagtttcaga atcatcggac ctctactttt tctgctaatt tgcaggaaaa aatacaaaat
    16801 cgattagaca aaaaagaaca aactgtccta ctgctcaatc gtcgaggata ttcgtctttt
    16861 gtgatgtgta gggactgtgg atttgttctt ccatgtccta attgcgatat ctctcttaca
    16921 ctgcatatgg atacacgttc aatgcgctgc cattattgcg gacatgaaga acctattccc
    16981 aatcgctgtc ctaattgtgg cggcaataaa atccgctact atggaacagg tactcaaaaa
    17041 gtagaagagg aactccgaga actttatccg caagctagga ttttgcgtat ggatgtagat
    17101 accactcgaa gaaaaggggc acacgaacag attttacaaa aattcggtgc aaaagaagca
    17161 gatatattat tagggacaca aatgattgct aaaggtttgg actttccaga agtgacatta
    17221 gttggggtgt tgaatgctga tacctccctg aatttaccgg attttcgttc tagtgagcat
    17281 acattccaat tgcttaccca agtttcagga cgagcagggc gagcggaaaa agcaggcgaa
    17341 gtcgtgatcc aaacattcaa tcctcaacat tacgccatag aattggctaa aaaacaaaat
    17401 tatgagcagt tttatcagca ggaaatgcat gtacggcatc gtggaggcta tcctccttat
    17461 tactttactg tgaaaataac cgccagccat cctgaagaac aagtagcggc aaagaaaatc
    17521 tttcagattg ccaatcagct aaaagaagtg ttaagccctc aaagcttgct attagggcca
    17581 acgcccagca tgattttgag agtgaaaaac cgatattatt atcaactaat catcaaatat
    17641 aaacatgaac cgaatcttcc tcatgttttg gatgaaattt tgaatggaag ccagaaagaa
    17701 cagcgacaag gtctttttgt ggcaattgac aacgaaccat taaactttat ttaagaaagg
    17761 gccaaattat gcgctatcct atattgatcc accctaatga taaattaaaa agaactgctc
    17821 agccaatcga tgtaatcacg gacgagacaa tcgctttatt agacaattta tatgagacaa
    17881 tgattgcgaa tgacggaata gggatcgctg ctcctcaagt aggacaaaac aagagaattg
    17941 ctgtgatcga agtagatgaa ggcgagaaat ttgaactgat taacccagaa atcatcgaag
    18001 caaaaggtga aagccttgac gtagaaggtt gtctcagtat tccgcatgtc tatggaacgg
    18061 tcaagcgagc agatgaagtg accgtgcgtt attatgatcg agacggagaa gagatagaag
    18121 tgacggcttt tggctattta gcccgtgcct tccagcatga aatcgatcac ttagacggta
    18181 tcttgttcat tgagaaaatg atacagcaga tcccggaaga agaacttgaa gaatatatgg
    18241 aggaacatct tgatgactaa gctagtattt atgggcacac ctgctttttc tgtgccgatt
    18301 cttgaagggc tgatagaatc aggttacgag atccaggcag tggtaacaca gcccgaccgt
    18361 ccagttgggc ggaaacgagt aattacgccg acaccggtaa aaaaagcggc tctgaagcac
    18421 ggtatccgtg tgctgcagcc agagaaaatc tccggttcgc cagaaatgga agaaatcatt
    18481 gaattggtgc ctgatgtgat cgtcactgcg gcatttggcc aattcttgcc tgaaaagtta
    18541 ttgcaagtac caaaattagg ggcaatcaat gtccatgctt ctttacttcc taaatatcgg
    18601 ggaggtgcgc ctgttcatta tgccatcatg aatggggaga aagaaactgg tgtaacgatc
    18661 atggaaatga tcaaaaaaat ggatgcaggc ggaatctatg cgcaagaaag cataccaatc
    18721 actaaacaag atgatgtagg gacgatgttt gaaaagctta gccttttagg aagaaagcta
    18781 ttgctagaaa cattgcccaa tattttggat ggacaaaaac cagtacctca agacgagtca
    18841 gaagcaactt tttcgccaaa catcacacgg gaacaagaag caatcgattg gcataaaact
    18901 gcagaagaaa ttgacaacca agtacgcgga atgcgtcctt ggccaattgc attcacgacc
    18961 tatgagggca tacgctggaa attattatcg gttgaagtat tggcagaaac tacagacaaa
    19021 gcacctggta cgatcatccg gaaagacaaa aaaaatctat ggattgcttg cggtaaacag
    19081 accgtcttag ccatcaatga attacagcca gctggtaaag gaaaacaggc cgtacatgat
    19141 ttccttaacg gaagcggtca gcaagttcag attggtcagc aggtgaaata atgaaaaaaa
    19201 tccctaaaaa aatgaaaaaa tcagtacgat atatggcatt actgacatta gagcgagtga
    19261 acaagggtgg cgcctactcg aatcttttgt tgaatgagat gatcaaacaa agcgagctaa
    19321 atcaaaaaga tgtgggttta tttacagaat tgatctatgg tacgattagc catcagcgac
    19381 tattggaatt ttacgttgca ccattgatcg ctaaagcaaa aa
//
LOCUS       DAYLZO010000055        18974 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.056, whole genome shotgun sequence.
ACCESSION   DAYLZO010000055 DAYLZO010000000
VERSION     DAYLZO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.056"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(59..1018)
                     /locus_tag="ACGVJG_001698"
     CDS             complement(59..1018)
                     /locus_tag="ACGVJG_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFJ6557640.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(1011..1961)
                     /locus_tag="ACGVJG_001699"
     CDS             complement(1011..1961)
                     /locus_tag="ACGVJG_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFJ6557641.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            2111..2563
                     /locus_tag="ACGVJG_001700"
     CDS             2111..2563
                     /locus_tag="ACGVJG_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFJ6557642.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(2652..4919)
                     /gene="gshAB"
                     /locus_tag="ACGVJG_001701"
     CDS             complement(2652..4919)
                     /gene="gshAB"
                     /locus_tag="ACGVJG_001701"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFJ6557643.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(5106..6104)
                     /gene="ftsY"
                     /locus_tag="ACGVJG_001702"
     CDS             complement(5106..6104)
                     /gene="ftsY"
                     /locus_tag="ACGVJG_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFJ6557644.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(6115..6924)
                     /locus_tag="ACGVJG_001703"
     CDS             complement(6115..6924)
                     /locus_tag="ACGVJG_001703"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFJ6557645.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(6933..10514)
                     /gene="smc"
                     /locus_tag="ACGVJG_001704"
     CDS             complement(6933..10514)
                     /gene="smc"
                     /locus_tag="ACGVJG_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFJ6557646.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(10534..11220)
                     /gene="rnc"
                     /locus_tag="ACGVJG_001705"
     CDS             complement(10534..11220)
                     /gene="rnc"
                     /locus_tag="ACGVJG_001705"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFJ6557647.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(11568..13349)
                     /locus_tag="ACGVJG_001706"
     CDS             complement(11568..13349)
                     /locus_tag="ACGVJG_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFJ6557648.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(13375..14289)
                     /locus_tag="ACGVJG_001707"
     CDS             complement(13375..14289)
                     /locus_tag="ACGVJG_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6557649.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(14316..15278)
                     /gene="opp4B"
                     /locus_tag="ACGVJG_001708"
     CDS             complement(14316..15278)
                     /gene="opp4B"
                     /locus_tag="ACGVJG_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFJ6557650.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(15278..16222)
                     /locus_tag="ACGVJG_001709"
     CDS             complement(15278..16222)
                     /locus_tag="ACGVJG_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557651.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(16223..17227)
                     /locus_tag="ACGVJG_001710"
     CDS             complement(16223..17227)
                     /locus_tag="ACGVJG_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557652.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(17495..17737)
                     /gene="acpP"
                     /locus_tag="ACGVJG_001711"
     CDS             complement(17495..17737)
                     /gene="acpP"
                     /locus_tag="ACGVJG_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFJ6557653.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(17859..18860)
                     /gene="plsX"
                     /locus_tag="ACGVJG_001712"
     CDS             complement(17859..18860)
                     /gene="plsX"
                     /locus_tag="ACGVJG_001712"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFJ6557654.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
BASE COUNT         5202 a         4142 c         3365 g         6265 t
ORIGIN      
        1 tgtacatctt taaccactga ttttccatgg tacagtttat tgaccttttg taaatcaatc
       61 ataggatact cttcctttcg cgaatcaatt ttgtgacaaa ataaattcct ccgacaaatt
      121 caacgacgac actcactgtt gtattccagc cgaaaatctg ctctactgcg agctgtcctc
      181 ctgtcagaaa cagcatagct accagaaagc ttccgataaa tagctgacca tgacgataag
      241 tagaaaagac acgataactg atatttgcga caataaaccc taaaaaagta actggaccca
      301 caagagccgt tgatgtccct gtcaaaagac tgacacaaca taaaatcaaa aattcaaaac
      361 gatctgtgtc tatcccgagg tttactgctt gttcatttcc gagatggaaa acgtctagca
      421 catgtcgata tcgaaataac aggtaccagc agccgataat gatcaaagaa gcaggcaaaa
      481 gatactgact ctggatattg ccaaagcttg caaataatct gccttgaagc aaatcatatt
      541 cattcggatc catgatgact tgtaaaaagg tactgacact accaaaaagc gtacctaaaa
      601 tcatccctga catcagtaat aaaaaaagat tcgctctcat ttttttcaaa agaaagaaaa
      661 ataacaatag actcagcccg accatcagcc caacgttcaa taagaaaaaa actgtacctg
      721 tctgctgaag catcgtcact cctccgataa agaagaaaag gaccgtttgg acaaggacat
      781 ataacgactc aagtcccaat actgaaggag tcaaaaactg gttatgcgta attgtctgaa
      841 agctgattgt ggcactgctg acagctagcg agacaaggat aaatgcaaaa attttcttac
      901 tcctcaatgc acatgcaaaa tgccagtttc catatgtatg gatcgtcaag tacattcctg
      961 taacaaataa aacaagaaca agcataccaa taaaaataga tggctttttt ttaagcatat
     1021 cgtcgtcccc ttatcagaag ataaaggaag atagccccac cgatgattcc aacgatcaca
     1081 ctgactggta tttcataagg aaaaatcaac acgcgagcga caagatcgca aaatactaag
     1141 aacaaactac ctgctaaagc agttacgcca attgttttgt gcacttgatc tccatatatt
     1201 ctagagacaa gattcggaat gatcactccg ataaaaggaa tgcttcctac catgatcaac
     1261 atgactgcac tagccaatga tactaagcca atgcctaaaa actgcatctt ctggtaatct
     1321 attcccagat ttactgcaaa cgattcgccc agaccaatga tcgtaaatcg gtaagcaaat
     1381 agccaaacca agagcaacaa tggaacaaca gcatacaata actcataatt tcctcgtatc
     1441 acagtcgcaa aattcccttg aagccatgaa gtcatatttt gtaccaactg gaattgataa
     1501 gcaaaaaaag tagcaactga accaatgata ttccctaaca tgactccaat caacggaagc
     1561 atcaattgat ttttctggag gaacaatcgc gtaagggata aaaaaagcaa tgttcctgca
     1621 aaagcaaagc aaaatgctgt caaggatcgg acaatcaagg atgcaccagg taggaaaatc
     1681 atagccacta aaatacctaa acgcgcacta tccatcgtgc ccgcagtcgt tggtgaaaca
     1741 aatttatttt gggtcaaatg ttgcatgatc aacccacaaa tactactggt cgctcccaca
     1801 aggatcaaac aaatcgttcg tggcaaacga gtcactagca agatttgtaa gctttgccca
     1861 tctaactgga gcagttctga caattgtaca ttcgatactc cgatgaacaa tgaaatacta
     1921 cttaaaataa ctaataatgc tactagtcgt ccgtagcgca tccaatccac ctttctaatt
     1981 gagaatgatt ttcattctta ttttaagtga atcgttgaac aaaagcaacc gctttttcat
     2041 atgttttagg tattattggg aatatgctat aatcgtaaga aagacaaaga gaagaaggag
     2101 ggtatttttt atggcacaag atgatgtaaa aagccatttg gataagggac tttatggtac
     2161 acctttagtg aaccctgaag aacagcataa atatttaggg acattcaggg aacgctgcta
     2221 tcttagtatg actgttgctg aaatgatcca accaaaaaac aaagaaaact ttttaaaaga
     2281 actcaaaaaa catccagatg cttccatact cttgaatggg gctctacccc tttctgtcca
     2341 aaaccaatat atccagctag caacgaaaaa caatagccgt ttcactgtag tcaacgatta
     2401 cgtcactgca aatcctgagg aattcggatt gctcttaaca gcaaaagaag cagtaaatga
     2461 acctgtgatt gatatcgaaa aaaaataccc agaagaaaac tcttcgccag ctgaaaaaga
     2521 aaaaccaaaa aagaaaaaaa gcttctggga tcgtttattt taaaacgaaa aaacacataa
     2581 atttttacat gcaaaaaaag cttttgatca gattgacatc aaataggaca atctgtcaaa
     2641 agctttttct tttaaaatac ttcaggatat aacaatttca atacattcat cgtcaatctt
     2701 ctgccttttc ctgcaaaagg ataaacatgc atatgcatgg ctggattgaa atttgcttcg
     2761 atgatcccat atgctttttt atcagttgta ggatcgattt ctttatctgg tataatcaaa
     2821 tcgataccac taatcttagc tcctagtgct tctaccgcag aaacggcgat ttttttgtaa
     2881 gcttcagaaa attcatcagt catatcgatc gaatcaccac ctgtactgat atttgagttc
     2941 tcccttagat aaacaatttg attggcttgg ggaactgatt cgatcgtcaa cccttgttct
     3001 ttcaacatca attgttctaa ttcacctaat tggatcaatt ctaaaggtgc acgatgattc
     3061 gttccacgca aagggtcact atttttttct tcaacaagtt ctttcactgt tcggatactg
     3121 tcaccaataa cattagcagg gactcgcaac atgatggctt ggacttctcc gtcgatcaca
     3181 aagaaacgat attccgttcc cggcataaat tcttctacta aaaccgaact gtcttcacga
     3241 aaagcaatgg ctaaacctgc ttgataatct tccagactag ctccttcctt gaaaatcgtg
     3301 atacctaacc cgtaattggt tgatttaggt ttgatcacaa atgcctgctc ggcaaatctc
     3361 ggataagctt ttaccgcttc ttccatggat gaaaattctg cgcctcctgg tacccggaac
     3421 ccagcttctt ttaagacttt tttcgttact gttttgtttt ccatgataag tgggacgata
     3481 tagctgtcct tacttgtcat gtttgcattt ttcacatatt ctacatggtc ttggaattgc
     3541 agacgcaaaa attgatccga ctcatctaat actttgactt ggacaccttt ttggatggca
     3601 tcgaacataa atatctgagt agaaagttcc atttcgcgaa aaccggccaa ctgataggga
     3661 cgttcatggc tttctttata atattgatgt cccaatgcca cagcaacttc ttgctgactc
     3721 gatagctgtg cttcttgata gagtttcgct gctaacgttt gttcaggatg atccatttga
     3781 gtcaatgctt cctctactaa ataaagagaa tccaaccaat ccaattcaac cagcatttgc
     3841 ttcattcctt caagaatttc tctaccttct tgatgaaatt ctgtttgatc tgatggttgt
     3901 tctagtgcta ctttgttatt tcgagtcgta ccttctgcta cccattggtc acagtcttct
     3961 ttttcttctg tccataacat gtacatcagg aataattgta agaaccgaac ttgctctgga
     4021 ttgataccca atcttgcata aggattcaaa tcgatattcc gcaattcaat ataacgaaca
     4081 cctgttgttg ctaaatctgc gactttcttt ccgcctcgga aacgaagcgg tgtataaaat
     4141 tctttctcct ccgaaagttt cccttcttcg atcattcgct cgatatctgc taaatactgt
     4201 ttcatagaag cataggaaac tttcacattt ggatgattcg tatagcccag cgcactgttg
     4261 cgaatactgc gaacaggttc ttgcggatgt gattcatcta aaaagtagtt cttctcactc
     4321 attggtgaag caccgaataa ataagtgatc atccagcgat agcgcataaa gtttctcgct
     4381 gttttcaaat aaagttctgt tttgaattcg gaaaaatctt gaaattcttc ctgatggctg
     4441 aacaatgttc ttagcaactc atcgccaaat tcaaaattga aatgaatacc gcttaccatc
     4501 tgttttcgct tcccgtattt ttttgctaaa taacgtcgat acaagacatc ttcgaaattt
     4561 tttaattttg caataatgat ctcttcgtct ttttccggta aggcaggtgg catacttaat
     4621 ggccagatca tctcttcttt cggtatagaa cgagcagtca catcataaac agcagcgaga
     4681 tactgaaata attcgggaat agaatcggta acaggggtga tcatctctgt ttgtgtttcg
     4741 ctaaaatctg tttggatata ggggtgatag gatcgatcgc caaagattgc tggatgatcg
     4801 gtttttgcta gatttcctgc aagatcaacc cgctgtcctt cccgttctat accaaatcga
     4861 gcttggtcga taaaagggcg cgcattgaca tgcaataata attgcttaaa attcatcatt
     4921 aagtgacctc caattcatat gaatctaatt ataatcaaaa agtctaagaa aaagaacgaa
     4981 attgtttgag aaccgcttac aaataagtat aaaaatgaag gcatcaaaaa cacagctatg
     5041 ttaaaaacaa tcattcaaac aaggagctgc aacttttgtc acagctcctg ctttttagac
     5101 atgggttatt cttcttttag taatcctttg aacaatccaa cgacaaaatc atttggatca
     5161 aacggctcca agtcatcgat tccttcacca agaccgacca atttcactgg taaatgcaat
     5221 tcattacgga tagcaagtac gataccgccc tttgctgtac catctaattt tgtcaaaaca
     5281 agaccggtca catctgttgt ttctttgaat tgttttgctt gaaccatcgc gttttgacca
     5341 gtggttgcat ctaatacaag aagtgtttct tggggtgctt ctggtaattc ccgctggatt
     5401 actcgtttta ttttttctaa ttcgttcatc aagttcactt tattttgcag tcggcctgca
     5461 gtatcaacta gtaaaacatc tgcctgctct gtctttgctc tttccaaagc atcgaatacg
     5521 acagctgctg gatcgcctcc tgcattgcca cgaacaactt caacaccagc gcgttcgccc
     5581 cagacaacta actggtcgat tgctccagcg cggaaggtat ctgctgctgc catcaatact
     5641 tttttacctt cgtttttgaa ctgatgggca agtttaccaa tacttgttgt tttccctaca
     5701 ccattaacac cgacaaacaa aataactgtt agtccgtctt cttggatatt tagttcattt
     5761 acttcattga ttccttccgc ttcatagaga tccacaagtt tctcgatgat cgcattttgg
     5821 acttctgccg attttttcgc atttctgagt ttcacttctt gacgtaatgc ttcggtgatt
     5881 cgtatcgctg tttcaaaacc tacatctgca ccgataagtg tttcttctaa ttcttcaaaa
     5941 aagtcttcat cgactgttcg aaaattggca aacaactcat tcatccgttc accaaatgtt
     6001 ttacgcgtct tttctaaacc cttgtcgtac ttctcctgta cttcaacttc ttctgttttc
     6061 tcttcgccag taaaagcacg cttgatttta tcaaaaaatc ccatttgact ccccctactc
     6121 taatacaaat ttttggatca ctttggctac gccattttct tcattgctgg ctgtcacgaa
     6181 atctgctgct tctttgacga aaggcacagc attctccatc gctacgccta gtccagcata
     6241 ttggatcatc ggtaaatcat tttcctcatc accgatcgcc atgatttctt cttgtttcaa
     6301 accaagatct tttgccaata aagaaatacc ataagctttt gtaacacctt ttggcataaa
     6361 ttccaaaagc atacttcttg ttttgatgat ctcatacatt tcatggaatt ctgctggtat
     6421 ttctttgatt ttaggatcta attcttcttg gtcataagcg attactgcct tattaagcac
     6481 aaattcatct gtaatatcgc ttatatccgc tggttggaat tgcagcagct tattcaacac
     6541 attgtacaag gattgcttcc ttggggcact aggcagctgt aaaaccacat tgtcagacaa
     6601 aacatctagt ggcagatcca attgctgagc cagtttgtat aagcgttgga tatctgatac
     6661 ttccatcact ttcttttcca tgatttcacc agtatcattt ttttgtacca aaccgccgtt
     6721 gaaagtgatg ctgtagtcac cttcatctac cagtcctaat tcttgcagat aatgcgccat
     6781 cgcagctaag gggcgtcctg tacatagaac gatcttgacc ccttgctgtt ttgcttgtgc
     6841 taatgcttgt ttattctcat ctgaaatcag cttttgttca tttaataatg taccgtccaa
     6901 atcgattgct actaatttga tcattttcgt tcctacactt tcgtttcgtt gattttcgcc
     6961 tcttcattga tatcttccat ccgtacagaa acgatttttg atacaccgga ttcctgcatc
     7021 gtcactccgt acagtacatc tgctgcgacc atcgttcctt tgcggtgtgt gactacgata
     7081 aattgtgtgt catcttggaa atcgctcaag taacgaccaa aacgtttcac gttggcttca
     7141 tctaacgctg cttctacctc atctaagata caaaacggaa caggacagac acggataatg
     7201 gaaaagagca acgcgatggc tgtaagtgct cgttctcctc cagaaagcaa acttagactt
     7261 tgcagttttt tgccaggagg ttgtacttca atctcaatac ctgttttcag caaatcagtt
     7321 ggttccgtca gtactaattc agctttaccg ccgccaaaca tatttggaaa gacaattttg
     7381 aattcttgac ggatagcttc aaatacttct ttgaatcttg tccgcacttc atcatccatc
     7441 tcatccatcg tttcaaacaa ttggtttttc gctgctaaca gatcatctcg ctgtgttgct
     7501 aagaatgtat gtctttcact gacttgttcg tattgttcga ttgaatttaa gtttactggt
     7561 cccagttttt caatctgttc ttttaaggat gaaacctttg ttctactgtc ttcgatatcg
     7621 gttgtttctt gatagtcttg ggaagctttc tcaaatgtca gctgatattc tgactgcaga
     7681 taggtcaaat gattatctaa aatcatttca gctcggtctt tttggacttc taatttggat
     7741 tgttccgaca gtcgtgcttt ttgctcacga ttacgctctg ccaagactgc ttcaagctta
     7801 tcgatctcct tttgccttct atctcgctgc tctttggctt ttgccaactc ggcttttaat
     7861 gtttctcgct gagcagataa ttcgttaatt tgtttttcca aactttcttc tgtgacttca
     7921 tgatcagaaa agtctgctgt caatgtcgcc aactgctttt ctatcgcttc ttgacgttcg
     7981 gttgcctctg ctttttggac tcgggcaccg cggagctgga tctgcagatg attaaactgc
     8041 tctttcaaga cagcaagatc tgcttgttct tgtgcttttt gactttgtac ttgtgctctt
     8101 ctggcttcca tctggtcgct ttcttgactt aacgatttga tttcttcgtc gattttttgt
     8161 cgttgcgatt caagatctgt ttgtttgtct ttcaattcgt tcgtttgctt ttcgtaatct
     8221 tcgataaact gctgcagttc acgtgtctca aaagttgaga tttgcttttc tttttcaaaa
     8281 cgttcaagtt catttatgat gttttgtagc tgatttgtcg cttcttgttc ttcaaaacgt
     8341 aattgttcgc ctcgtgtacg caaaacttct tgttcttcgg ctaaacgagc tgtttcttgt
     8401 tgcagctcct gaactttttt ttcttgtgct tggagttgtt tatctgcttc ttcaaattca
     8461 ctagtcaact gtttcaattc ttgattttgt acaaacaaac tgccttggtt tcctcgttta
     8521 ttcgctccac ctgtcatgga tcctccggca ttcatcacat caccttctaa ggaaaccact
     8581 cgatattgat agcggatcgt ttgcgcgatt gcattggcac tagttaagtc tttggctaaa
     8641 aggatcgttc ctagcaggtt ttgaaccaca gtttggattt gatcgggata actgacttgt
     8701 tcactggcaa tccctataaa cccttctaca gctgctgctt gtgtcaatat atgagccggt
     8761 aattgtcttg gtttgattgt cgtcagtgga aggaatgttg ctcttcctcc tctttgctgt
     8821 ttcaaatacg tgatggcttg ccgagcatct ttttcatttt caacgatgac atgctgggct
     8881 gccccaccta aagctgcttc gatagctaac gtgaaatctg caggaacatc gatcagttca
     8941 gccactgctc cgacaatacc cgaaagctgc tgtttatgct gcaagaccaa tcgaacccct
     9001 tggtaaaaac caaaatagtt ttcttggatt tcttgaaggc ttttcttacg agcacgtagc
     9061 tgctggactt gattcattaa ttgatacatt ttaggctgtt cttttgctaa tgcttcttgg
     9121 aactttttcc cgttttgttg gatcatttct aacttctctt ttgtttcagt taaagatgat
     9181 tttagctgcg cctgcttttg tgtcaattct tctttttgta agatcaatcg gtctactgat
     9241 gcttctactt ccgattgctt ggctaacgtc tgcttgcttt ttgctgtttc ttgtatatat
     9301 tgacgttcta gatacttcag ttcgtttcct acagctgctt gttcttgcat caaatcaaca
     9361 tattgatctc ttaattctgc caataattct ttcgttgatt tttggtattt ttctaattct
     9421 tgctgtgttt ttatgagatt tgcttcagct ttttggatct ctgtttcttt ttctgctgct
     9481 gctttcatca agctttcttg aagcttttca aaatgtttaa cctttttttg tgcttctgct
     9541 aatgaagttt gatattcttg cgagcttttt tgtgtatgct ttgtccgctc ctgcaaaaca
     9601 tctttttgtc cttctgtctg cttcagtttt tccgaaagat ctaataatac ttgctgattt
     9661 ttttcgatca gacgatcggc ttgcgcattt tctttacgct gcttcgccaa aatggattct
     9721 tgttcttgga tcgactcaga tagtttgcct aattctagat tgaatttcgc aagctgtgct
     9781 tgtttgttgt cccaatcttt ttttgctgtc ttgatttcag caaccatcaa agagacatcg
     9841 gtttgagtca gtgtttcttt tagccgtaaa aactcctttg ctgcctcact ctgggcggcc
     9901 aaaggagtta actgttcttc caattcgtgg atgatgtctt ggacgcggct gaggttgtct
     9961 tctgtttcaa aaagcttctg ttctgctttc tttttccgct gcttgtattt caagacacca
    10021 gctgcttctt caaagatccc acgacggtct tccggtttac tactgaaaat cgcttctact
    10081 tttccttgag aaatgattga gaacgattct tttcctaatc cagaatctaa gaacaattct
    10141 tggatatctt ttaatctgca gctttgttta ttgatgaaaa actcgctttc cccagtccgt
    10201 cgataacggc gggttacact gatctcattg tactccaatg gcagataatg atcactattg
    10261 tctaaaatca ctgtgacttc tgcaatattc agctgcttac gggtatcgga accggcaaag
    10321 atgatatccg gcatttttcc gcctcgcaga cttttagcag attgttctcc taatacccag
    10381 cggatagcct ctgtaatatt gctttttccg ctgccatttg gtccaaccac tgctgtgacg
    10441 ctattttcaa aatcaatgac tgtcttgtct gcaaatgatt taaaaccggc tatttcaatt
    10501 cgtttcaaat acaaagcttt ccctcactta cttttacagt gctgccaatg cattttccgc
    10561 cgcagcttgt tctgctaatt ttttcgattt cccttgtcca gtgccaatca attgatcatc
    10621 tacataaact tcgatccaaa atactcgttc atgagctgga ccttcttcat tgatcaatcg
    10681 gtattcgatt gatacatcac ctgatttttg caagacttct tgtagttttg ttttatgatc
    10741 catctcatgt gaaaaagcac ctgctttgac tttaggaaaa atcacttttt cgatgaatgc
    10801 atgggtcgtg tcgaatccct ggtcaagata taacgcgcct aaaaaagctt cgaataggtc
    10861 gcaaagcaat gctggtcgcg tacgtcctcc tgaattctct tctcccttgc ccaacaaaac
    10921 atattgatca aagtgacatt ctttggcaaa tttcgacaag ctgtcttcgc gaacgatcgc
    10981 agctcgtgtt tttgtcaatt ttccttctgg caaatgagga tataaacgga ataaatactg
    11041 agaaaccatc agttctaaaa cggcatctcc taaaaattca agtcgctcat tatccgataa
    11101 ttgcagattg cgatgctcat tcacatagga tgaatgggta aaagcttgtt caagcaaatt
    11161 tagatcgtga aaaacgatgt tatagttttc ttttagttct tttgtcagct ggttgtccat
    11221 tttccacatt ccttttatgg atattttttc gatacttcct tattatactg gttttttgtc
    11281 aaatttgaaa tggttcggtc aaaaggtaag taaagcttta ataatcctag tgattgacga
    11341 gaagttgtga aaaattcgtt tagtttcgag tatgaagaaa aaattttgtg aaaatggctt
    11401 tccatatttt tgcataaatt tgacttaata ccgagaaact ggactttgaa caccgtttat
    11461 tggagaagtc atgaaatcag cgccttgacc tgaatattag agaaataaac agaaaaatat
    11521 caaaaaaccg aaaaaggaca tcgccctttt tcggtcattt ttagtgatta tgaacgtgaa
    11581 tctgctgtaa cagaaacatt tgcccatggg tttggtacat cataagacca atcccagtct
    11641 ttgacacggt cgttgactgg caatacttca ttacggaaca acgttggaat cgcaaatgct
    11701 tcttcaaatg catattgttg ccatttttga tatgcttctt tttggtactc ttcatcaaat
    11761 gctttgcttg aactgatatc gttgatcaat tttgtatttt cttctgattc ataacgagtg
    11821 tagttgaacg cagcatttgg tccccatagt ccacttggat ttggatcagt tcctgtattc
    11881 caagcagctt ggtaaacatc aatttcagga tcatcgtttt ctaatttatc atagaaagaa
    11941 ttgaattcga tcagacgacc tgttgtgtat tggacatcca aaccaatttc tttccattgt
    12001 tggatatagt aatctgccaa tggttgcgct gtttccccac cggacatcga agcaaagttg
    12061 attgttagtt tatttccatc tttgtcttct cttaaaccat cgtcatccac atctttgtag
    12121 ccagcatcat ctaatagttt tttcgctttg tctagatctt gtgtgaatcc tttcatctca
    12181 tcatcatggt atgactggaa tactggagga atcaatgaag tcgcattgct tctcaaacct
    12241 gcatagaaac gttcacctac tgcattatta tctaatgcat agcccattgc ttgacgtaat
    12301 gatttatcag ccatttttga atctggattg tattccacac tctttgtatc tgcattccat
    12361 ttacctaact tgaatcctag ataagtataa gaagtttgtt cacggcctaa gtttgtatag
    12421 ccagcaattt ctttataggt atcgtaagta tctgttggca tttggaaaat catatcatat
    12481 aatttagagt tcaatgcttc agttgcactt gccatcggta cagatttcat cgtgatcttt
    12541 ttcaatttag gcgtaccatt ccagtagtgc tcgtttggca agtattctac tgattctcct
    12601 gcaacgatat tactcatgta ataagggcca aaagtcactg gatttttacg tacttgatcg
    12661 cttgattcca tatctttgat tgctacacct tctaacgtat gttttggtgc tgcatatgac
    12721 cagataccac cacccgattg aagcatgctt gggtttactt ctttatattc gatttcaact
    12781 gttttgtcgt ctattttttt gattccggag attgtgtcgg cacttccatc atgatattct
    12841 tccataccta cgatgttacg gaaagtgtcg tcataacgga taccagtgta atctttattc
    12901 ccaatgattt cgtaagggaa aatcacatcg tccgccgtta catcttcacc atctgaccat
    12961 ttgacgttgt cttttacttt gattgtcgct tttttcgtat cttcatctag atcaagcgtt
    13021 gcagctccgt cgtctgtgat tctgaaatcg tcatcgtttg caaatagcgg ttcatgagaa
    13081 ggagccataa aagctgcatc atatgaatct tggtaaaact cccactggaa caatccttta
    13141 aactgactgt ccgttgcgac cgcagcttct aattcgccgt cttcgattgc atcatcatta
    13201 ttttttgttg caattggaaa agttaattcc tctttgttat tgccgttgcc tccagagttg
    13261 tttccagagg tatctgtacc tccgcccccg catgctgcaa gaacgacagc agacatcagc
    13321 gctgcaaatc caaaaatcgt tttcttcatt cctatttccc ccttcgcttc tatactagcc
    13381 taaccgttgg cgcgcatctg cagaacgctt gaatgcttgt ccaacataat tgatactcaa
    13441 catcaaaact aaaataagga ttgatgcggg taaccagatc cattgccgat tcactaagac
    13501 ttctccgtta cttgcataac cgatcaatgt acctaaacta ggtacatttg ccggcaagcc
    13561 gaaacctaag aaagtcaaag ttgtctcgat accgatgttt gccgcaaagt tcatcgtcaa
    13621 attagtaatg attaatgaac ttagattcgg catgatttcg cggaacatga ttttaaaatc
    13681 attggttccc attgtttttg aggcactgac ataatctctt cttgcttcag ataatgtctt
    13741 gcttcggaat aatcgggctt tagctaccca ataaaatgca ctcatgatga atataaagga
    13801 ccagacattg tatctggaaa taatcgatac gaagacgata atgatcagca tgatcggtag
    13861 aatcatgata aaatcaacga tccgcatcaa gatgttgtca atgatccctc cgtagtagcc
    13921 agagatgatc cctaatccaa ccccgatgat cgaagtaagg atcgtgatcg caaatccgat
    13981 cgtaacagag tttctagcac ctatgatcaa ttgtcctaat acatcacgtc cgccttcatc
    14041 tgctcccaga atgaactttt gtccttgtgt cgaaactgtt cctggtgcag cgtatttgtc
    14101 gaagatacta acatacatca ctttgtcttg atcggtcagc atcgcaccga taaagatcac
    14161 taataataaa accactagca aacctaatga aaaaatagca attttatctt ttttaaattc
    14221 tcgagcaatc attcgtatcc ccataggagg aatactttct tgaactgctg tattgttttt
    14281 ttcactcatc gatccattct cctttctgat tggttttatt gaatccgaat acgcgggtca
    14341 acgatactca tgatgatatc tgaaagtaat gtccccacta gcgtcgcgat tcctaaaatc
    14401 aatactaagg atgtgatcac ggtatagtcc cgttggccga tggaatcaat aaatagtttt
    14461 ccgactcccg gataaccaaa aatcttttcg ataactacag aaccagcaat caacgaagtg
    14521 atttcatacc ccatttgaga agccatcggc aacgctgcat ttcggaaaat atgtcgactg
    14581 aatactttat tcgtcggtac ccctttggaa cgggctgttc tgacaaaatc gagcgatttc
    14641 gaatcgatta cttcacttcg cagatattgg attgtgacgg cagtcgccaa aaatgcttgg
    14701 gtaattgatg gtaacagaat atatttcaaa cggtctatcc attgagtgat aaatccacct
    14761 tgcaaaccta gtgtttgtga tccacttgtt gggaaccagc ctaaacgata accaaaaaca
    14821 aatagcatga tcaagccgaa gataaataaa ggaacagcaa aactaaagaa gttgtagatt
    14881 acgacaatct tatccaagat tgaattttga taccggccag caagcatccc cagaggcaca
    14941 gcaatcaaat aagtaaggac aactgtcaag atcgatagat ataaagtgtt gatgatccgt
    15001 tcgccgatca agtcagcaac agagcgtttg tataagaaac tttgaccaaa atcgccttgc
    15061 aaagcatttc cgatccaacg gaagtactgg acataccatg ggtcattcaa gccagctgct
    15121 tgtcgcatct gttcgatcac tgccggatcc tgatttggat tgatcaaacc ggtaaatgga
    15181 tcccccggca tcgcttgggc taataagaag atcagaacac ttaaaataat aacttgcggg
    15241 atcatcaata aaacacgtcg cagaatcgtt ttccacatta gcttgcacct ccatctttca
    15301 acgccacttt gtgtgtagtt gtgatcgtac gcaaatcgta gactcgacca gtcgcatcat
    15361 agtattcttt ttggttttgg atatactctt gttctactct tcgtcgattt tctttgtggc
    15421 tttccctatt ttctacatcg atttgcggaa tagctgaaag cagacgtttc gtatagatat
    15481 gtcgcgggtc attataaatg tcttctcttg taccgatttc tacaaatcga cctttgtaca
    15541 taattgcgat attgtcgcac atgtgtttta ctacacctaa atcatgtgaa atgaataaat
    15601 agctgaggcc gtattcttgc tggattcgtt tcatgaaatt caatacttgc gcttgaacag
    15661 atagatccag agcagataca ggttcatcgg ctacgatcag ttttggattg gtagcaacag
    15721 cacgggctac tcctagacgt tgacgttgtc cgcctgaaaa ttcatgagga tatttatata
    15781 gcgcatcttc cggcatccca acgatatcta gcagtccttt tacctttttc ttttcttctt
    15841 gatcgcttaa ccgttcaaaa tttcggatgg gttcagcaat gatatccaac actcgctttt
    15901 taggattcaa actggacatt gaatcttgga agatcatctg cacatccttg ttataaccca
    15961 tttttttgcg gttgcttcgc ttcgttacat cttttccttc atacatgatt tgtccggctg
    16021 ttaccttttc taatcccaca acggcttttc ccgtagtaga ttttccagag ccagactctc
    16081 ccactaaacc atatgtcttt cctttttcga taataaaatc cacaccatct acagcatata
    16141 catgatccgt tactcgattg aagaatccac tgcgaatagg gtaatgaact ttcaggtctt
    16201 tgattgtgat taattctgtc atctacacct ctcctttttc gtctctaaag tggaaatgct
    16261 tatagcagga gcaacgtacc aagtggttcg gtgcgacttc atgaaggata ggctcttctt
    16321 catgatcgct tgctggtatc catggaatac gtggtgcaaa ccgacaacct ttacgaggca
    16381 tgtttttcag agaaggaacg acaccttcga tcacatggag ttctgcatca tgatttcctt
    16441 cttgtggtat agagttcagt aaagagcgtg tataaggatg cttaggatga tcaaacagtt
    16501 cttttacacc tgctacttca acgaactgtc cgccatacat cactgccact cgatcagcca
    16561 tttctgctac gactcctaag tcatgagtga tcaagatgat ccctgttttt gtttcttcct
    16621 gaaggtcttt cagtaaatca agaatctgtg cttggatggt tacatctagg gctgtcgtcg
    16681 gttcatctgc aatgacgatt ggaggtttgc aggcgattgc gattgcgatg atgacacgtt
    16741 gtctcatacc gcctgatagt tcatggggat attggcgtcc aactctcgca ggattaggaa
    16801 tccctacttg ttctagcaat tctagtgcac gagcttgacg ctgttctgca ttcatctttg
    16861 tgtgatagga aagtccttct ttgatctggt cttcgattct catgagagga tttaatgcag
    16921 acaacggatc ttggaaaatc attccgatat catttccgcg gattttatta tagagcgttt
    16981 cgttcaggtc gattaaattc agatcgttat ataaaatctc acctgtaatt tttgtgttat
    17041 taggatcaag taatcccata atcgtagtag ccaaggtact tttcccgcac ccagattccc
    17101 caacgattgc taaaatctcg tttttgtcta aagtaaatga aacgtcatct actgcatcgt
    17161 aatagtcatc tttgagtcgg aatcctgtat gtaggttctg aacttctaat aattggttat
    17221 cggacaatcg aatacccctc tctgcttcct aaatggaagt ttttcttaat aaaaaaggac
    17281 attacacaga ataaagcgtt ttataacaaa ggttattctt atctttgtgt aatgaattat
    17341 taataattat atagaaaatc gctttacttt gcaacataat ttctgtttat cgccagaatt
    17401 atcagaaaaa tgacttttac gtgtcaaaaa gattaaaaaa agaaggataa aaagctgtat
    17461 gcagcttttt atccttcttt gcttttattt gaaattattt aagatggctg ctgatataat
    17521 ctactgcgcc accgactgtt tcaatttgtt cagcgtcttc atctgaaatt tccgtaccga
    17581 attcatcttc aagctctaaa acgaattcca taatactaat tgaatcagct tttaaatcat
    17641 ctttgatgtt caattcatcc gtcactttat cggtatctat ctcaaaatga tttgaaataa
    17701 ttttcgctac tttgttaaat acttcttcgc gagtcaactt tttcacctcc aagaatcata
    17761 cacttgtcaa ttacctacac catggctaat tttactggca tttttcagga atgtccacct
    17821 ttttttcaat ggaacggatt gcttcagata tccaaatact attcttcgct tttttcaaat
    17881 tgttcgacta attgtccaac tacatctgtc tcaagcattg tgtggatttg gcggatcgta
    17941 tagcgaacag catcggggcc tgttgctcca tgagttttaa tgactggtgc tttcaagcca
    18001 aacaaaacgg caccgccatg tttggaataa tccatttctg ctttcaaaga acgtaagccg
    18061 tcttttaaaa gcatagcgcc catttttcct ttgatccctt catttagaat agcagatttt
    18121 aatagattca tcatattcat tgctgttcct tcaatagatt tcaaaacagc gttgcctgta
    18181 aatccatctg tgaccactac atcagccaca ccattgagta gctcacgagc ttctacgttt
    18241 ccaataaaat tcaagcttgt ttcattctga aggagttcat aggctttttt cgtcacttca
    18301 ctaccttttg ttgcttctgt tccgttgttt aagagtgcga cacgaggacg tccgatacct
    18361 cggactttac gtgcataaaa agaacctaaa ataccgtatt gcaacaaatg ttctggtttg
    18421 ttttctgcgt tagcgccaag atccagcata tcgaaaccgg caccttcttt ccctactact
    18481 ggcaaggtag acatcaagcc agggcgttcg atatttttga tacgtcctac gatgaacaac
    18541 cctgctgcta gtagcgcgcc tgtattccct gctgaaaata cagcatctgc ttcaccgttt
    18601 ttcactgctt gtgcagcaag gaccattgat gcagtctttt ttcgtcgaat tgcttttact
    18661 ggctcatcat cactattgat tttttcgtct gtatggataa tcgtgatgtt tttttcattt
    18721 gttaagtatt tcttgatttc tgcttctttt ccgtaaagta agaattctac ttctggaaaa
    18781 tcttgctgcg ctaaagccac gccttctaca attgcttgag gtgcgtgatc tccgcccatt
    18841 gcatctacgg caattttcat tgtttccact cctttttccg tcatcagtat aaagtatagc
    18901 acagtctgct aatcgaaata acgattatcc tgattttcta tttggaggct ttgcgctaaa
    18961 ggctgatact cagg
//
LOCUS       DAYLZO010000056        18575 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.057, whole genome shotgun sequence.
ACCESSION   DAYLZO010000056 DAYLZO010000000
VERSION     DAYLZO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.057"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(367..933)
                     /locus_tag="ACGVJG_001713"
     CDS             complement(367..933)
                     /locus_tag="ACGVJG_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFJ6557655.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(933..1889)
                     /locus_tag="ACGVJG_001714"
     CDS             complement(933..1889)
                     /locus_tag="ACGVJG_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFJ6557656.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1889..2545)
                     /locus_tag="ACGVJG_001715"
     CDS             complement(1889..2545)
                     /locus_tag="ACGVJG_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFJ6557657.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2915..4933
                     /locus_tag="ACGVJG_001716"
     CDS             2915..4933
                     /locus_tag="ACGVJG_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFJ6557658.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5003..5209
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5360..7774
                     /gene="leuS"
                     /locus_tag="ACGVJG_001717"
     CDS             5360..7774
                     /gene="leuS"
                     /locus_tag="ACGVJG_001717"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFJ6557659.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8122..9675
                     /locus_tag="ACGVJG_001718"
     CDS             8122..9675
                     /locus_tag="ACGVJG_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFJ6557660.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10056..11840)
                     /locus_tag="ACGVJG_001719"
     CDS             complement(10056..11840)
                     /locus_tag="ACGVJG_001719"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557661.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11855..13576)
                     /locus_tag="ACGVJG_001720"
     CDS             complement(11855..13576)
                     /locus_tag="ACGVJG_001720"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557662.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13676..14437
                     /locus_tag="ACGVJG_001721"
     CDS             13676..14437
                     /locus_tag="ACGVJG_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFJ6557663.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15404..15672
                     /locus_tag="ACGVJG_001722"
                     /pseudo
     CDS             15404..15672
                     /locus_tag="ACGVJG_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16344..>18575
                     /locus_tag="ACGVJG_001723"
     CDS             16344..>18575
                     /locus_tag="ACGVJG_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFJ6557664.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6104 a         3299 c         3745 g         5427 t
ORIGIN      
        1 ggggcataat ttgactgata ttaagttagc tattgatata tgaatgacat acaaaaatat
       61 aaaatctttt aaaaatgctt atatatcaat gtttttgcca ttcttttata tatctgttat
      121 atgtcaaaaa aatgtgttca atttcaattt gtatttgaat aaagaggttg tgaaaaagct
      181 gtcctgcatc aagtaataag aacagccaat caaaaacagc ttctcatgtt tttgatttgg
      241 ttggacttat taccgcagat ataagctttt gagcaccgtg aataaagagg ttatgaaatc
      301 aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca tatcatttat gttttgcgtt
      361 ttttatttat ctttttttct caatacaata taaaataggc ggctggttct tttggttgat
      421 aaaccgataa ttcagcacat tatatgtttc ctgtggcagt ttggaacaaa aagaatcaac
      481 catatccagt tctttttctc caccttcatg accatagtag acgacaagga tcaatcgtcc
      541 gcgaggcaca agtcgtttca atatttcttc catagctgtg cgtgtagtct ctggcatagt
      601 gatgattgct ttatcacttt ttgggagata ccctagattg aaaatccctg ctttgagatt
      661 ttgttgttca gctatcactt ctcctaacgt ttcgtggcct tgtagaaaca atgagacacg
      721 ttcttctaac tgacgctgtt ctagcctttc acgagtagca tcgattgctt gttgctggat
      781 atcaaaacta tagacatgac cagtttttcc gatgtgttct gctagaaaaa ccgtatcgtg
      841 gccattgccc atcgttgcat ctacgacgtg atcccctggt tgcaagattt cttgcagtaa
      901 tgtatggctg aaatgcaaag cagtttgcaa catcagacgc tcaccttttt ccgtacatca
      961 tattttcctt ggaagctatc tctgcgaaga agttcttcat caattgcatt caagacttcc
     1021 cattttttca agctccacat tggtccaatc aaggaatccc acggcgcatc ccctgtcaaa
     1081 cgatggatga tgatctcttt tggtatcatt tccagctggt cgcaaatgac attgacatag
     1141 tcaggacggc tcattaattg aaggcgacct tcatgatagt cccgcaacat tctcgttttg
     1201 cgcatcagat gcatcaaatg gagcttgatt ccttggatat ctgaatctaa aatggtgcgt
     1261 ctgacatttt ctttcatcat ttctaaactt tcgcctggta atccgttgat caaatgcgta
     1321 caaacacgga tattatgttt gcgtaattta gccacgccat ctaaataagt ttgataatca
     1381 tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg tttgcaatcc aagttctacc
     1441 cataaataca tccgctcgtt cagttcagca aggtaatcaa ctacttcatc tggcagacaa
     1501 tccggtcgtg taccgactga taatcctacg acacctggta aattgactac ttgctcaaat
     1561 cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa aattttggaa ataaacgatg
     1621 tattgttgga catgaggcca tttttgatgc atcatgtcga tttctttctg gaattggatt
     1681 ggaagagggt cttcaggtgc aacgatcatg tctcccgaac cagatacact gcaaaaagta
     1741 caaccgccat gggcaacagt cccatctcga ttcggacagt caaatcctcc atcgatcggc
     1801 accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt aattccaaga atgatatctt
     1861 ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt ctttctgtaa gagttatcta
     1921 tttgttttct tttttctcta tacactggat aagtcaggca cagccaagaa agactgagta
     1981 agtaaccgcc gatgatatca gtaggaaaat gaacgcccag ataaatgcgg cttatcccga
     2041 cacttactat aataaacatc atcagcaatt gaagcaatgt gcgtaaggtt cttttttgaa
     2101 caaaagctgg aagcagaaat agtaaagaac cgtaaagaat catactgcct gctgcatgtc
     2161 cactagggaa actgtaactt gtttcaacta ctaagtgcgc caatgaagga cgctctcgca
     2221 tgaaaaatag tttgatcacc gggttgatga ttccagcaat tacaataaat ccagaaacga
     2281 accaaaatgt ttctgccctt tttttcttat accacatcca taaagccgca caagcaaata
     2341 aaatgatgac ggtaagagga ttaccgaatt tagtcataaa gagttggtag ctgttcagat
     2401 caggataaga agctctgaca atgcctgtaa accactgatc gaaaccagaa agccacgaca
     2461 gatggaactt tacgatccag cccaaaatga aaaacaggac aagaaagaag ctaccagcca
     2521 cctgaaatga tgttttatat ttcatacaat tcctcctttg aagcaatgac ttattatagc
     2581 atttttggtc cagcgaaaaa aacggtaaag agataaaacc aatgtatgat tcctctttga
     2641 ataaccaata ttcttataaa aaaaataact gtaatcggaa agacattttt tggtaatgca
     2701 aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat ccttttaaac aagaacgtag
     2761 acgtaggaag aacttgaaaa tgaacagaag ttgttcatta cggtaaatta caatatgatt
     2821 acaaaaaaca acaaagctct taaataactt ttaataaata ttaagagaag ttataattat
     2881 taataataga taaattgaag ttggaggata gaagatggaa aacattgcaa gaaaagaacg
     2941 gagacgttta gaacagacca aacgttttcg aaaagcaaaa cgaggggcag caatcgtggg
     3001 aactgcgatg gtaggctgtt ctgtcgctgc cccattagta cagccggttc ctgttcaagc
     3061 ggatgaaaca cctgttcagt tcggcgcaag aatcaataca gcggcattta tcacagaaat
     3121 tgctacttat gctcaaccaa tcgctcaagc caacgatcta tatgcatcag tgatgatcgc
     3181 acaagcagtt gtagaaagcg gttggggcag tagtgcgtta tctcaagcac cttattataa
     3241 tctttttggg ataaaaggca gctatcaagg acaaaccgtc tacatggata cgcttgaatt
     3301 tttaaataat caatgggtaa ataaaaagga gcctttccga caatatcctt cttttgctga
     3361 atctttcaat gacaatgcct atgtacttag aaatacttca tttggcaacg gttattacta
     3421 tgccggtact tggaagagca acacgaaatc ctatacagat gcgacagctt gtttgacagg
     3481 gcgttatgcg actgatcctg gttacgcagg caaattaaat aatatcatcg caacttacgg
     3541 attgacccaa tatgatacac cagctagcgg aaacgcaggc ggtggagcga ctacaggtgg
     3601 caataatact ggaagtgcag gtagtactgg cggttcaagc aattctggta atacaaactc
     3661 aggttcagca acttcgacca cttatactgt ccaatctggt gattctgttt ggggaatctc
     3721 aaataaattc ggtatcacaa tggatcaatt gatccaatgg aacaacatcc aaaacaattt
     3781 catttatcca ggacaaaaac tgacaatcaa aggtagccag gaaaatggct cttcgacgaa
     3841 taattccggt aacaatacca attcttcagg aaatgctggt acatcaaacg gaggacaaac
     3901 aactggtgcg aaatacactg ttcaatctgg tgattctgtt tggaagatct caaatgatca
     3961 cggaattacg atggctcaat tgattgaatg gaacaatatc aagaacaatt tcgtttatcc
     4021 aggacaacag ctaatcgtat caaatggcgg ttcttctaat gctggagcaa atactggctc
     4081 aaatactggg aatacaagtg gctctagttc gtccagctca agttcttcta atacagcttc
     4141 aggaacgaaa tatacagtta aagccggaga ctctgtttgg agcgtctcaa ataaatacgg
     4201 aattacgatg gctcagctaa tccaatggaa caatatccaa aataacttta tctacccagg
     4261 acaacaactg actgtttcaa acagtggatc aacaaacagc acgtcaggta atacaggttc
     4321 gtcaaccaat acaaataaca gcagttcagt taatactggc agcagttcgt ctcaaggcac
     4381 ttataccgtg aaagcaggtg aatctgtttg gagcgtggca aataaaaacg gcatcacgat
     4441 ggatcagtta atcgaatgga ataatatcaa aaacaacttc atttatccag gacagcaatt
     4501 gatcgttaaa ggtggaactt cggcaaatac aaatacaggt tcaactactt ctgcagcgaa
     4561 accaaataca ccgaatacgt ctgctacgac aagtacatca tccactggta atacgatgta
     4621 taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac aaacatcata tcacgatgga
     4681 tcagttaatc gaatggaata atatcaaaaa taatttcatc tatccaggac agcaagtgat
     4741 tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca gttactggtt cgaaaaccta
     4801 tactgtgaaa tctggagaat ctgtttggag tgttgctgac agtcatggaa ttacaatggc
     4861 tcaactaatc gaatggaaca atattaagaa caactttatt tatccaggtc aaacgttgat
     4921 tgtagctaaa taaaatagct gttcgaagaa ataacgattg aattccacaa aaatatttgt
     4981 tactataagg ctacttaaat atgacaacac ttgaaaagga agtagtaaaa agactgaagt
     5041 ttcagagagc gcatggctgg tgagaatgtg cacggatgct tttgaactcg cctttgagtt
     5101 gcccttgctg aatacccgag taagcaagcg gcggtgacgg ccgttacaac gttcgaggcc
     5161 ttttcactaa ggtaaaatta ggtggtaccg cgtagattac gtcctataaa aagagcaatc
     5221 tttttatagg atttttttat ttcttttgtg aagtttgaga aaacaaagca aaaggaaata
     5281 aaaataaaca gattctgccg gttttttcgt tatatttata atagaaacaa gtgttttcaa
     5341 tagaacagga ggacagcaaa tgagttacaa tcataaagaa attgagaaaa aatggcaaaa
     5401 atactgggca aaaacgaata ctttcaacac ccatgatgat cctgaaaagc caaaatttta
     5461 cgcactggat atgtttccct atccatccgg tcaagggcta catgtagggc atcctgaagg
     5521 ttatacggca acggatatcc tttcacgtgt gaaaagaagt caaggataca acgtgctgca
     5581 tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa tatgcgctag atacaggaaa
     5641 cgatccagct gagtttaccc aaaaaaatat cgaaacattc cgacgccaaa tcaattcttt
     5701 aggatttagt tatgactgga atcgcgaagt gaatacaact gatccggagt attataaatg
     5761 gacacaatgg atctttacga agctttatga aaaaggtcta gcttatgaag cagaagtagc
     5821 ggtaaactgg gtacctgaat taggaacagt tatttcaaat gaagaagtga tcgatgggaa
     5881 aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg cgccaatgga tgctgaaaat
     5941 cactgcttat gctgatcgct tattggatga tctagaatta gttgattggc cagaaagcat
     6001 taaagaaatg caaagaaatt ggatcggtcg ttcagtagga gcaaatattg aatttaaagt
     6061 ggctggtacg gataaatcct ataccgtatt tacgacacga ccagatactc tattcggtgc
     6121 aacttactcg gttcttgcac ctgaactaga tcttgttaga gaaattacga cccctgaaca
     6181 aaaagctgct gtagaagctt atatcgaaga gacagctaaa aaatcagatc taaagagaac
     6241 ggatcttgca aaagaaaaaa caggagtatt tactggtgct tatgcaatca atccagttaa
     6301 tggcaaggaa atcccaatct ggatcgccga ttatgtatta tcttcatacg gcacaggagc
     6361 catcatggct gttccagcac atgatgaacg tgactttgaa ttcgctcaaa ctttcggact
     6421 agaaattctt ccagttatcg aaggtggaga tgttgaaaaa gctgcttaca cagaagatgg
     6481 tacgcacatc aactctgaat ttctagacgg catgaacaaa caagaagcaa tcgataaaat
     6541 gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa gtcagctatc gcttacgtga
     6601 ctggctattc tcccgtcaac gttactgggg tgaaccaatc ccaatcatcc attgggagga
     6661 cggaacagtc acagctgtcc cagaagaaga gctaccatta cggttgccta agacaaaaaa
     6721 catcaagcca agcggtactg gagaatctcc attagcaaat atcgaggagt gggtcaatgt
     6781 cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg aatacaatgc cacaatgggc
     6841 aggaagctca tggtattacc tgcgttatat cgaccctcat aacaaaaaag aactagcaga
     6901 ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac attggtggag cagaacatgc
     6961 ggtacttcat ttactatatg cacgattctg gcataaattc ttatacgata tcggtgttgt
     7021 tcctacaaaa gaaccattcc aaaaactata caatcaagga atgatcttag gcgaaaacaa
     7081 cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct gatgatgtgg tcgaaactta
     7141 cggtgcagat acactgcgca tgtacgaaat gttcatgggg ccattagatg cttcgattgc
     7201 ttggagcgaa aatggcttag aaggaagccg taaattcttg gatcgtgtat ggcgtttgat
     7261 cgttgatgag aacaataaaa tgcgtgaccg tatcactaca ttgaatgatg gcaagcttga
     7321 taaagtgtat catcaaacag tgaaaaaagt gacagaagac tatgaaaatc ttcatttcaa
     7381 tacagcaatc tcccaattga tggtctttat caatgaagcc tataaagtgg acgctctgcc
     7441 ttatgaatat atcgaaggat ttgttcaact actcgcaccg attgctcctc acatgggtga
     7501 agaactatgg gcaatccttg gaaatgatgg tggtatttct tacgcaccat ggccaacata
     7561 cgatgaagca gcacttgttg aagacgaagt agaagttgtt ttccaagtaa acggtaaagt
     7621 tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat gagctagaaa aagcagcatt
     7681 agccaatgaa atagtgcaag aatatattga aggcaaaaca gtcagaaaag ttattgcggt
     7741 accaaacaag ttagtcaata tcgttgctaa ttaattggct ctttgtcaat aaggactgat
     7801 gagttgtgca aaatcaaatc tgggccagaa tgaacttcat tctgtctcag attttttgtt
     7861 atttcaaatt gattaattga ttgatcaaaa aggttttgat tttcatgttc tcaaacacgt
     7921 tcaatgtctt tttcttctga atggtcttgt aagagatctc tgtaatcatc aaatttttgt
     7981 ctattaatct tagtattttt tgatgtataa actacctgaa attgagagta cataccaaat
     8041 tcataaattt tttattagtg ggactagtga ttgtatggat tttgacgcat aatgaatgaa
     8101 gtatgagaaa aggagagaca aatggatcaa gaactattta ttatcgaagt attatcagct
     8161 aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa gaataatgtt caagctttta
     8221 agcttagtat ttttctctgg tttgcttttt ttcggagctg tatcgttcct atatcgccat
     8281 atgagtgcag tagtccgttc agctgaaggg atgcaaaaat taaataattt gtatcacttt
     8341 tcgtggggag ttggcggaat aacaattgtt gtgcttattg tttttcttct acttatttgg
     8401 ctccgttacc atcaattgtc gcgtcatttc gatcaatggc ttagtcaaag agcacaacag
     8461 tttttacata ataaaccaac ttctcaagat gatgactatc tttattttga gaagacttcg
     8521 acgagtcaag cttttcggat acaaaaaagt gattgccagt tattgctgag atcacttgat
     8581 gatttaccta tctacttagg cgaaagcaaa tcaaaattcg gaatgaacac attccatatt
     8641 tttttactag aaaacgaacc cagatgggaa aaaagccagt cttcgcctgg tggatacctg
     8701 acaactaaga tgagtacatt agtcactggt tttttcctca ttcttttagc tggaacaatc
     8761 tattatggga gatacactca gccaaaaatg tcttctcata taaatgctgc ttctcacatg
     8821 acaatgaact caactaacga tacgcatata caaaagacaa atgaagggtt gttagctgat
     8881 cctaagcaaa aggtaaattt gacaacacaa aagataaatg atctcaaact tgatacagag
     8941 acgcactctc tctatatgac aacagatagt ggtgctaatt gggtatttgt gccgattgat
     9001 tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg ggctgattcc tgaaggttat
     9061 tggatggaca acacatatga tatttccgcc gatttctcat ggtttatcta ttcttctggg
     9121 gatgatgtac gtctgctgac ttcaagagat aatggtaaaa catggcaaac gtctattatc
     9181 acccaatcag ttcagccgat acgttatcga aaagcggtat tttatggcaa cgatggtcta
     9241 tttatctgta catcagccag tgggatgtct gcagaaactt tgtacattta cactactatg
     9301 gatggcggaa tcacttggat aaaaagtgga agtacaatga tcaatcagcc agtacaaaat
     9361 gtcagcttcg ttacaacttc tttaggtttt gtttcgacac gtgaaagttt gttttataca
     9421 aacaacggag gacgctcatt caaagaatca gttctgtcga ttccggaaaa ctatcagaca
     9481 ggggggatag atctttttca atcaccaaat gaagttactc aaataagcgc gaatacgtta
     9541 gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg cagggaaaat gtttgcctgc
     9601 ctcttccgtt caactgataa cggggaaaca tggcaattcg ttcagcaact atcgcaagta
     9661 gaacaaggag attaaagaag agagattgtg agcctgacgt tcagcaccga gtcacaagaa
     9721 acaaattcaa aaaacagctt ctcatatttg ttgaatagtg taactcgtga ttcggagttg
     9781 acaagagcaa caagaagcga ttttgaagta tagcttctcg tattttacaa gatcgtgtct
     9841 tattgccgat aggttgtctc ttgaacaccg tttattcgta aaaagggatt gtgacaagtc
     9901 ttttgtcact acttaccgaa cgaaactgaa ggagctatcc aaaaagtatc attagtatta
     9961 ttctttgtgt ttctagtggc accactagta catttaaaaa agctatcgat catggactaa
    10021 tcatacatga tcgatagctt tttagataat gtttgttacg ctatctgaaa ttgactatta
    10081 taaagactgg cataaagcgt tggtgattgg agtagttcag aatgtgttcc tttttcgacg
    10141 atatcgccat tcttcattac aaggatcatg tctgcatttt caatcgttga caaacggtgt
    10201 gcaatcacaa aacttgtcct atcttttgtc acagcttcca ttgcttgctg aatcaatgct
    10261 tctgttcgtg tatctacgct agaagttgct tcatctaaaa taacgacagg aggattcgct
    10321 agaatgactc gagcaattgt caataattgt tgctgtcctt gagataacgt gccgttttcg
    10381 ctagaaatga tcgtatcata tccgtctggc agtgttcgaa taaagtgatc acattgtgcg
    10441 atttcagccg cttcgataat ttgttctctt gtggcatcca ttttcccgta ggcgatatta
    10501 tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa ccatcccaaa agaacgacgt
    10561 agttctccac gtgaaagctt ggtaatatca agaccgtcga attcgatctt tcctttattc
    10621 agttcataaa aacgcatcag cagattgact aaagtcgttt ttcctgcacc agttggacca
    10681 acgatcgcta ccgtttgttt tggctttact gaaaaatcga cttgattcat caatatttta
    10741 tctgatgtgt aaccaaactg tacatttttg aaagctattt ttcctttcgg ttgttctaaa
    10801 agtcttgcat ctggaaggtc tggaatttca tctgtttcat ctaatatctc aaatatccta
    10861 tcgattgctg ccattgcgga ttggatcgag ttgatcacat aagaagcggt agaaattggt
    10921 tcagaaatct gattgatgta ctgaagataa gcttgtaaga agccgatcgt cacaccacct
    10981 gatagaacaa gcatagcacc gaataccgca cttatgatga aggcgagttg attaatgaaa
    11041 cgaatggccg gatagatagc acagttcata aattgagcac gtttaaaagc gttgtattgc
    11101 tttttattta cttcgtgtat agcagcttct gcatgagctt gctgattgaa tgttttgacc
    11161 actagattac cagctaagat ttcttccatt cgattattta aaacacccag ttcatactga
    11221 ttctgttcag aaatctgttt gtttttgttc gcaatcttag ttgtaacaat tgaactgcca
    11281 acgatcagca ataagacgag caaagtaagc ttgctttcga tataaaacag catcagacca
    11341 gcagcagcta ttgttacgac agaagtaaaa aattgattga tacctgtcaa taacacttga
    11401 gaaagttgat tgagaccggt agttgtccga ctaatgatgt cacctacttg atgattgtca
    11461 aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt cttttcgcac cctcaatgtg
    11521 atcttttcgc ttaacgctgc cattgtccgt tcctgaataa atgaagtgat gctgcttaag
    11581 actgaacagc cgatcagcaa taagacaggg aacaacaatg cttgtttgat atcactgaaa
    11641 gaaagctgtc caatacctgt ttttcttatt tctgataaga gatcatcgat cccgattccc
    11701 atgatcaagg gcatggctac gactaatagg ttcccgaata aactgcatag aaacaatccg
    11761 taaaaatatt tatgttcagg tttgatcatt ttccaaaaac gtatcaaagc acgcgtgttc
    11821 aacgtttttt tcttttccat cgtatccctt ctttctatat tccttgtgat tttgcaaact
    11881 cctgataagt ttggcaatta gacagtagtt cttcatgagt accttgtcca acgatttttc
    11941 cttcatctaa aacaatgatg ttatctgctt gttggattgt actgaggcgt tgagcaacaa
    12001 tcacgagagt tttctccgac atgtgtttat gaagggctgc tcttaatgct gcatctgttt
    12061 tatagtctaa agcggagaaa ctatcatcga atacatagat ctctgctggt ttgatcaacg
    12121 ctcgagcaat acatatccgc tgtttctggc cccctgaata attagaaccg ccttgagcaa
    12181 caaagctgtc caatccgtca ggaagggtgt ccactagttc ttctaattga gcgatcttga
    12241 tcgctttaga caactcttct gttgaggcat attcatttcc catctgcaag ttgcttaaaa
    12301 ttgtcccgct gaataagaaa gccttctgag ggacataact gatgtgggag cggatctgtt
    12361 cttgagtcat ttgagtaaga ggaataccac caagggttac ttttcctatc gtagggtcac
    12421 taagtctcaa gagaagtttc gccaaactac tttttccaga tccggtcccg ccaacgatcg
    12481 ctgttgtttt tcctttaggt attgtgaaat caatatcttc tagaactgga tcagagattt
    12541 cagtatattt aaatgtgaca tgttcaaaag cagctgcatt tgtctgatca aaggttggtt
    12601 cagctactgt tttgatcgtc tcttcctcaa tttcgatctc tgcttgcagt atttcttcta
    12661 tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa taccatagct gcgatcatca
    12721 agtaagcaag tgtcagcata gagtattcga ctacagcagt cacgacaccg atttgtaatg
    12781 ctccttctgc tacaagattt ccgccaaacc acaaaataga agcataaacg attcccatga
    12841 gcaaaaaagc aattggagtg acccaagcaa atattcggtt cactttgatc atttgattag
    12901 catagtcagc aaagctctta ttcgttcgct tttcttcata tgattgatta tcaaaggcac
    12961 ggatcatatt aatcccagta aagaattctc tcaatgtgat ggtgatccga tccatttttg
    13021 gctggatact caaagataaa ggtgcccctc gtttcatcag tcgataaaca atccatacat
    13081 aaaaaacaac agagatcaat gtaacccatg ttagtaatag agaataacga aacatcatga
    13141 gaatggtgaa aacgcaaata accggtgctg gtaagatcat ttggcaaaat aaaacaatca
    13201 tttgctggac attatcgaca tcattcgtca ttcttgtcaa aagagatcca gaactaaaac
    13261 gactgatatc cttcattgac agctgttgga atttacggaa atatttctcc cgcgtttgga
    13321 atccaaacct agcggaagcc aatgctgaaa agtaactaga ggcaactgca gcgattgttc
    13381 ctagaaacgc tacaagcagc atttcaactc caattttttt tataactgag gcttcgccac
    13441 ttgctatacc tgtgtcgatc agctttgcta ctaattttgg tacacctaat gtaccggcga
    13501 tttctaaaca gatcatcaca aaggtaagga gagcgagtcc tttgtttttc tgtaagaatg
    13561 tttttaagat agacatatta ttcctccttt tttcctgata cttttcgatt ctataccttc
    13621 aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa aagaggtgag aaaaaatgaa
    13681 aaaaatgtat acgattggcc aagtatcgga actatttgat ttgccaaaat caacgatccg
    13741 ctattgggat gaacaaggat taatccgttc aagtagacaa gaagagaatg attatcgatt
    13801 gtttgatatc gatgatattt ttatgcttta tgatatcgtt ttttatagaa aattagatat
    13861 accgatcaaa cagatgaaga atttatatgg aaaaacattg acggagttat atgagacact
    13921 tgatgaaaca gaaagaagaa tccataaaga actggtggtg atgaaacaaa aacaaaaaga
    13981 gataagggag caaaaaaaac agttgaaatt gatgatcgat acaaacgagg aagaatttcc
    14041 tgtagaagag atcccttttg actgtatgat ctcaacggag tttgaagata ttgtggaaat
    14101 caaaaaattt ctacctaact attcaagctt tgggatgatg agtatgccag cttccagttc
    14161 acagacgatg tatggatttt ttatcgaccc tagtgaagta catttattta ctcaagacgt
    14221 gatttgggag aaaaaagata cggctgttta tcgtcgattc ttattgaaat ccgagatgaa
    14281 ccatgcggaa cgtaataata ttttagaaat cagagaaaga atgtatgaaa aaggatggaa
    14341 aacaggagaa gtgatcggcc agtatctatt gacgaatacg gatgaaaaca atatcagaac
    14401 tgaatattat catgcatgga tagaaatgaa aaaataggaa atatgtgttt aagataactc
    14461 gaaaatgatg gttgttttag ttataaagaa aaattttctg gttcgattac gggtttaatg
    14521 tgggggatta ttagtcgtgg atagatagaa ttttagtttt ttagaggaaa tatgtcttta
    14581 aaaatggaat ggttgcaatt cttagcgata tatacgatgt aattgtcctc ttaaaaaagt
    14641 atggaaaaat aattgtattg ggtgttatgt ataattcatg gtgaactgag actaatgaga
    14701 agaaaaattc atattgttat gagtgagatt gtatcagatg agaatgataa attaaatagg
    14761 tgcataaaag cattacactt aaaatacatt gttgcttaca ttattacaat atgttaataa
    14821 ggcaaaaata aatagatgga ttgaataaac atatttttta atcaaatagt ccctactgtg
    14881 actttcctca gctatcggtg atactaccga tagcattttt ttgcttataa aataattata
    14941 agtatttaga gatttttctt ctaacattct tcaaacaaga aaaatcacaa aggttattgt
    15001 tatttttata tattcttttt aagaatgatt gtttttttat agaaatttta aaaaataaaa
    15061 cacgttattg atggatagtt agtttgttat ttggtaatat tatagaagtt gactggtagt
    15121 gatttatttt tcgtcgtttt ataaaaatgt tattttttgg aaaaattcac aaatatgtaa
    15181 cgaatgtttt tctggaattt gctgtgttaa aaagcagtat gactaatata aatactattg
    15241 tcatttatgc aattttttat ggatagtaat tttagagggg aatcaatgaa ggaagaatag
    15301 aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata gcaatacatc ttcaagcttt
    15361 aaaattcgag agaatcatct tggtagtgga atcaactaca acaaaaggat taaaccaatt
    15421 aatagataga attaccaatg gaaaaataga aaaggtagtt attctataca aaagttgttt
    15481 aattcgcttc ggttatgaac ttagtgaaac cctttgcaat atatggtaca gaatatagaa
    15541 attattgaca ttacagaaaa tgatgaagat ttaatacaga ttgtcactat ttgtagatac
    15601 cttttacaag gaaaccgagc caataaagct aagaagatga ttaaggagtt gatggaagac
    15661 gatacaagtt aagaaagtga aactacgccc aaataaagag caaaaagagt aattggacgt
    15721 ggaaaatgca agaaatgaac catcgattcg gtggacagtt tattagtaat gacggtttga
    15781 gaacatccat gacgagaatg aaggaacatc ctaaatgcgt ttagttgaac caagtgccaa
    15841 ataatatggc caaataagcg atcagttatc gatgaatccc aagtattcta aactatatat
    15901 tcattttgat gctaagcatt gatatatttt tttgggattg atgaaggtta accaatacag
    15961 ggactcactg atgaaattct agagtctgac cttggtatca acgatttagt gattgtttct
    16021 aatggaaaag tataaaagta tataagtaca ttaacaaaat aactattagg gttacgctcc
    16081 agcttgtgca aggcactggc aggaggaatc gtgaaatcat aattatggtt aagccacacg
    16141 gacaactaaa agtcatacaa agctatcgaa tcaatcaaca gaagacaatc ggcaatcatt
    16201 agctttgttt acagcttaca ttgcaacgct tgagttgaac cgaaaatacg agggaggtaa
    16261 cgcagccatt ttgagttggg gaatcttttt agaaaattat cgggtacctt gttgtttagt
    16321 caaaattata agggggagta atagtgagga acaaatttca taaactgtta agtatgtttc
    16381 tagttatgct tattgtgatc aatcaatttg ggaacataac ggttctagct gagtctttag
    16441 cagaacagcc agagcgttcg ctgacgatcg aagttgatca agaggaggct gctttgggtg
    16501 agcagttcca gttgcgagtc agaggaacac cagacctgct aaatgcattg gtcattgata
    16561 agagtgagga tttgattgaa ggggaagtga agcaactcaa tgattacgaa cgtctgatta
    16621 cttattcttc ccagcattca ggtaaatacg aattgaccgc aagaagcggt gatattcaat
    16681 caaatacggt ctcaatagaa atcagtgtac cagaagaaag cacaagtgag tcttttaatg
    16741 taccagaaga aagcacaagt gaatctttta atataccaga agaaagctta agtgaatctt
    16801 ttaatgaagc cagatcatct gagccatcta taacgcagga atctcagatt gaaagacaag
    16861 aggagctctt gaattatgac atcggcatct ctgctgagcg ccccactttg ccgagcaggc
    16921 atacacaaac gttaaaggga tacattcaag gaggcgctgg cgatattctt gaagaatatg
    16981 gggagatggt cgtacgagtt cctagagcat cgattggctt aattgagttc attagagtgg
    17041 aggtcgctga tgattcacct ttaaagccac tggcatttta ccaagaaggc acagacgttg
    17101 tttttccatt tacctttgat tatgaaggat taaatgaagc cgctcagtcg tatacattca
    17161 gtatcacgtt tgaagttgct ttctttcaat caggcggtac tgagccaaat aatgactttc
    17221 aagtgagtct tacttatgct gagcgagcag caaacacgga attttcaatt acgcctattc
    17281 cttcatcaag acccattttt tcgaaattat actatggctc tagagaaatc gacagccaag
    17341 gaaatcttgg gaatctgctg attgacgggc aagacgtgac caacagtaat aaatttattt
    17401 tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt ggttaccgat gtactaccgc
    17461 aaggaacaac tttggcaccg aatccgacta catggagttc cactggaaat agagaaccta
    17521 tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg tgaagcaaac gttagaactc
    17581 attatgtttc cgaggaattt gtggacaaag tatcttttaa taaggaaacg aatacacttg
    17641 ttgtggactt tggtaatcta gcagagggtc aatcgtattt tattgaatat gctcttgaag
    17701 tgaccgatgt cgacttgttc aatgaaggaa gacaagagga tgggttttct cggaatcgag
    17761 cgagtatgac ctacaataat caggtaatga gccgagacta ccgcttgaga atccctgaaa
    17821 gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa aaatgttatc aatctcaatg
    17881 aaaatgaact aacgtatact ctgcatgtct cgatgttttc tggaacaaga aatacgcttg
    17941 ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc atttctatca gatattccct
    18001 ctttcattga tgccgaagtc aataatggaa tagttcgttt cagtgcgaat aatacgattg
    18061 gtggtccgac aagttttacc ctctctttta aggtcaatgt agaggcgtat gcggtcggcg
    18121 atcaaatttt aaactttgca acgcttgtga atagtgacaa tcgattgaat acgagtactg
    18181 taagaacaag gaaaatcgat gggcgtatta aagtaattaa aactgatgag aatggccaaa
    18241 ggctggcagg agcaaaattt gaaattttat caagcgattc acaagtgata caagaaggta
    18301 taacggcaga aaacggagaa tttttaagtg aaccgcttcc tgtaggtagc tatcaagtcc
    18361 gagaaacaga agcaccggaa gggtatcttt tagatgcgac ggtacatgat gtttttataa
    18421 atgaatactc catagaagaa gtgacgttaa ccattgaaaa catccttgat acgggtggtg
    18481 ttgaactaat taaacatgct gaaggcagtg gtgaagtcct tcaaggagct gtttttgagt
    18541 tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DAYLZO010000057        18211 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.058, whole genome shotgun sequence.
ACCESSION   DAYLZO010000057 DAYLZO010000000
VERSION     DAYLZO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.058"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..405)
                     /locus_tag="ACGVJG_001724"
     CDS             complement(<1..405)
                     /locus_tag="ACGVJG_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFJ6557665.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRRFRETTVFEDIFAYILEQAVKA"
     gene            complement(1736..2401)
                     /locus_tag="ACGVJG_001725"
     CDS             complement(1736..2401)
                     /locus_tag="ACGVJG_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557666.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2527..4563
                     /locus_tag="ACGVJG_001726"
     CDS             2527..4563
                     /locus_tag="ACGVJG_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFJ6557667.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4567..5205
                     /locus_tag="ACGVJG_001727"
     CDS             4567..5205
                     /locus_tag="ACGVJG_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557668.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5233..5661
                     /locus_tag="ACGVJG_001728"
     CDS             5233..5661
                     /locus_tag="ACGVJG_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557669.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6118..7029
                     /locus_tag="ACGVJG_001729"
     CDS             6118..7029
                     /locus_tag="ACGVJG_001729"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFJ6557670.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            7246..7683
                     /locus_tag="ACGVJG_001730"
     CDS             7246..7683
                     /locus_tag="ACGVJG_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557671.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7778..8662
                     /locus_tag="ACGVJG_001731"
     CDS             7778..8662
                     /locus_tag="ACGVJG_001731"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFJ6557672.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8886..12053
                     /locus_tag="ACGVJG_001732"
     CDS             8886..12053
                     /locus_tag="ACGVJG_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFJ6557673.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            12121..12618
                     /locus_tag="ACGVJG_001733"
     CDS             12121..12618
                     /locus_tag="ACGVJG_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFJ6557674.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            12637..13101
                     /locus_tag="ACGVJG_001734"
                     /pseudo
     CDS             12637..13101
                     /locus_tag="ACGVJG_001734"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            13098..13856
                     /locus_tag="ACGVJG_001735"
     CDS             13098..13856
                     /locus_tag="ACGVJG_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFJ6557675.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13942..14682)
                     /locus_tag="ACGVJG_001736"
     CDS             complement(13942..14682)
                     /locus_tag="ACGVJG_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFJ6557676.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14743..15996
                     /locus_tag="ACGVJG_001737"
     CDS             14743..15996
                     /locus_tag="ACGVJG_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFJ6557677.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            16318..16545
                     /locus_tag="ACGVJG_001738"
     CDS             16318..16545
                     /locus_tag="ACGVJG_001738"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFJ6557678.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16668..16994
                     /locus_tag="ACGVJG_001739"
     CDS             16668..16994
                     /locus_tag="ACGVJG_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFJ6557679.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            17062..17775
                     /locus_tag="ACGVJG_001740"
     CDS             17062..17775
                     /locus_tag="ACGVJG_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFJ6557680.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            18085..>18211
                     /locus_tag="ACGVJG_001741"
     CDS             18085..>18211
                     /locus_tag="ACGVJG_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HFJ6557681.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPE"
BASE COUNT         6226 a         2944 c         3629 g         5412 t
ORIGIN      
        1 tgctttaact gcttgttcta aaatataggc aaaaatatcc tcaaacaccg tcgtttctct
       61 aaatcggcga acataattct ttccaaaagt tgagaaatgg gggattttct cctcgaagga
      121 gtagcctaag aaccagcggt aagcgacatt ggtatcgact tccttaatgg tttgtcgcat
      181 ggaacggata ccgaataaat actgaataaa caccaatttg atcagaatca ctggatcgat
      241 acttgggcga cctaaatcgg agtaagtggc ttcgacgatt ggataaataa attcaaaatc
      301 aagcgcggca tcaatctttc gaacgagatg atcttgagga actagatcat caatcgtaag
      361 catcgcaaat tgatttctac cattcgtatt gtttttagac atcatagcta tcactccttg
      421 cattcgtttt cataaatcta ttataaaaga aaagactgta gatatcatcc ataaaatgga
      481 ctttgtctac agtctgagga ctacctgtat tagtcctctt ttttgaccag attttgacca
      541 gtctatttta cattctaata tattcatatg agctaatttt atgattaaat ggtaagtgtt
      601 tccttgtttg ttttagttta aactagtgtg taatttaaaa atctatatgg aagggtctgt
      661 ggggtaatac ttaaataaag gctaggaacc tgataacatc aaggttccta gcctttttat
      721 aataaaatga agcgaaaata atttctttta aagagccgag attatcagct atataagagt
      781 gaatttgatg caaaaaacag agccgaaaca gtcttggtgt tcgtagctct gcttatatgt
      841 ttcgaattta agacaaagag ttggcaaaag atgttgataa ggaagataag ataactgtgt
      901 tatataaact aggtaactaa ctgtaacaag ctaattttaa gagattctta ttagaagacg
      961 tttaggaata taactaagaa aatgattata caggtaattg tcatttcaat aatgttttct
     1021 aattttcgcg tctttgtttt aagaaaaagg ccagaatcag ttggaaaaaa gttcgtacga
     1081 tagtgaagag gggcaaaatt tcctactaat attcccacta acaagagata aaacggccag
     1141 gttggtgtga ttcctctata aaaaaatgcc catgctaccg aaatcccata gattaacaac
     1201 tgaacaattt ttacaatgaa aaaattgttc tgccattact caaagtctct tcctccttgt
     1261 atcgtttatt tgtttcattt tatcattatg ataggcgctt gaacaatcca ttttgtattt
     1321 tattgagtac tcttggataa actaccagcg attatcatat ttatttatgt tactgtaagc
     1381 catcatataa aagtaaatga gcagatcata gaaatataaa aaagaactaa aattgatcta
     1441 aattatgtgc agcttgacta cgtaccaatc tatattactt actagattgg tcgttaatct
     1501 attcggatcg aatgagcaaa atatttttgt gaatgtccat ccttgattat gattccaaaa
     1561 agaatcattg ggaggctgtg gcgtaaatat tatactataa gccagaaata cctatcattc
     1621 tgaacttttt tatttaggaa ctttcatctc aaggcttttt caattaattt gtacccctgt
     1681 tttttggata agatcatgtg cattgatttt ttctatctct acgttcatct cctacttaat
     1741 gcctatctgg ctcctgaggg cttcttttta aggcgtcgtc atagtcttgg ctgaggattt
     1801 cttggtaatc tgagggaatg gcttcccaag tgagcaggcg aacgttcatg acgtgttttc
     1861 ctttgtgttt gattacagca tggtttttct ctttttctac ctctccagcg tgaaaagaca
     1921 gccatgtacc atggccaaag atatcattac atacccaacg ttcgatggaa ttgtccacgt
     1981 atcctacagg gagtgtggta tagccgattt gtggtttcaa aaaaggattg aatcgatcga
     2041 tcgtttcatt tttgtattga taattgataa gaaaaagata gaccaaacaa aagacgagca
     2101 gaaagaaaga tagtgtcttt ttgagtgaga agggttgttt tgatgaataa gatttcttgt
     2161 taacaagagg ttcatctggc aatgttggtt ctattgattc ggtagctgat tcaatagaag
     2221 gaaaatcaat cggcagacct aaagtatgat gtaaaagtga cagcatttca aggtctggca
     2281 ttgtacgaga attttcccaa ctagaaatcg ttttatcgga aacaccaatt ttttcgccta
     2341 attctttttg cgtaagctta caatttctgc ggtaagttcg tattttttct ccggtgttca
     2401 tcttcttctc ctttcgattc tacagtatat agatttattc tacatgacct atacacttga
     2461 tagatacgtt tttttgatga aaaacggtat gattttatca aagaatcatt taagtaaggg
     2521 agagtggtga taatcaaaaa aaagttcaca gttttactca caatcatagt gaccggtatc
     2581 atgagtggtt ttgccttggt tagtataaat aaacaggtac tctatcggac aaatgaattt
     2641 ttccatcaaa cagatactta tactccgatt cgattgccac aacattttat tgggcaagaa
     2701 gaatatgagc agctaatcat ggtatttgaa aatgtctcat cagaaactga gctttcttat
     2761 atgaaaaaaa cagtaaatga atccaatgaa gtagatacat cttggaactt caagatacag
     2821 ccaaagcttg aaatgacatt gtattatcaa ggggatgtca aatcttctca cgagaaggta
     2881 ccctattcta atacctcctt tcgcgtaacg tccattgaag aatcgatcaa aataaaagat
     2941 tttgaaggtg aatttttctt gatgacgaga gaaaaagaag cctatgaacg gttcatttca
     3001 ctattcactg atcgattcaa tcaagcattt cacactgatt atgccactaa tatttttgaa
     3061 gactttgaaa atagctattc gtattcggtt atttcaaata cagacgtata taattatcga
     3121 gtgtatttaa ctattggatt gattctttta ttccttttgt tatcatcctt tttcttattg
     3181 atgaataaag aaatcagttt attgttgaat aatgactttt cattacatca agtattgtgg
     3241 tgtttattgg ggaaaaagaa aatcattatt acattcataa ttgcaggaat cattggtggt
     3301 tttattttta tgtattataa ggagcaattt ctttatttgt tccagatgta cgttttcttg
     3361 ttcagcggaa tatatggtat ctctttgatt tctcttttat tagttgaaaa aatcgcttcg
     3421 ttaagagaag cgttcaacta tatccaatac attcttttta catttggatt gttgttttct
     3481 cttcttttga ccgttgccaa tgtcgaatta gcaactatcg ttttttcttc ttcacagtta
     3541 gctgtttccc atgaaccacc aaaagaaatc gctggtgagg actatcatgt attttatcca
     3601 gtaaccatcg ggaaaaacca cgtggatttt atttattcca atcgttttgc atcagcagca
     3661 gatcaggaat tatatgaaac tttgaataaa aacggaagta tattagtcaa tgtatcagat
     3721 tacttgaatg aagaaaatga acagtttggc agagggataa aaataaatct gaattatctt
     3781 aagaaatttc ctttgattga tgtctctgga aggttgatcc aaataaccga aaaagaaacg
     3841 caccctgtta tattgatccc tgaacgatat agacatacag atttaaaaaa tgacctggcg
     3901 caaataacag aatattatca agaaatcagt gaaaaagaac cgaaatttct atttataaaa
     3961 aacaatcaac ccatttatac atttattcct tctatcccct ggatcgaaca ttatccggtt
     4021 gttgaaatac tgacattgaa aaacagtatg tatttggaaa gaaatattct cagcggagaa
     4081 atctatccac cgttaaagat caagataggc tctttgtcga aagaaaggtt atttgagctg
     4141 attgaagaaa gtcagttacg tgataacttg cagttatcat ttccgtatac tcagactgaa
     4201 gagatagctg tcaaagtgtt aagcagatca tttcattact tgatacagat atttttactc
     4261 actttctttt ctttcttctt gttgagctat gtcatgttat gcatatattt tgtctacaat
     4321 tgtcggaaaa tcgctatctt tcgttcaagt ggttattcgc tctttgaaac gtataaagat
     4381 ttcttcatga tgaacctcat caaatgggga acgacaagtg taatcttcct ttttcttata
     4441 gaaagagagc ctaaatatct ttttaatatt tttttcttta gcttcattga ttttattctt
     4501 tcagttgttt ttattttgtc tacagaaaag aaaagtcagt tattgctgat gaatggaggt
     4561 taacaattgg agccgttgat agtgataaaa ggtgcgaaaa aaatcattcg aaacaaaacg
     4621 attcttcatt tggatcgttt cactgcggat gctggggatc ttgtgactgt tgttggagaa
     4681 agtggagcag gtaaaagtac gttattgaat atattaggat tgaatgatca gttcagttca
     4741 ggacaattct atttgttcga tcaagagacg aaacaattaa ctgggagaga gaagcgcttg
     4801 ataaaaagaa ataccatatc ttttcttttt caagattttg ggttgattga atatgaaaca
     4861 atcgatttta atttagatat tggattgaag ttttcgaatt taaatgtttc agataagcga
     4921 acagcaaagt atgaggcatt gaaaaaagta aatataaaca agaagctaag tacgcctatc
     4981 tacacattat ctggtggtga gaagcaaaga atcgcctgtg cgagagtctt attgaagaat
     5041 agcaaaatca ttttagccga tgaaccgact gggtcattgg ataaaagaaa taaggataaa
     5101 gtagttgaaa tcttatcgca acaagcttcc caaggtacgt tagttatcgt agtcacgcat
     5161 gatccatatc tgaccaaaat aggaaacaag aagatcatgc tttaaaaatc aaaaaggaaa
     5221 ggatgtcgtt ttatgaaaaa agcagttatt atttgtgcac tagggatagg ttttgttgga
     5281 ggaaacgcta tgtcatctga agtggaagca aattcagttc acggtgaaat ctattacaaa
     5341 tctttaaagc caggagagat tcattttgat gctaaaggta atattattaa gtcaaattta
     5401 ccagtagacc gggaaatttc tagaacagca attaatcata gctctggaag atgggtttat
     5461 tatagtgata caacttgtac gagaaaaaca ggtcattcta accactatag tcgtgtctat
     5521 aatcgtcacg gtgcaaaagc gaaagttgga aactctattc gatcagcaaa tgcaacaaaa
     5581 ggagtatggg cctatgcaag tgcgacagga aatagaactg acacattctc atgttggtac
     5641 aatccaaccg gttggtatta agtatccaaa agtttatata aaatagaaaa aacatataat
     5701 gaaacagcgt taaaagttat ttttggtagt tttaatatat tcatttttag ttgtataaaa
     5761 atatatcata ataaaaacaa caaaaaaatg tctggaaaat cgaacataaa agtgtctgat
     5821 ttgaatatcc cagcagcgga agaagaaaaa caaaatcgat aacatttgtt ctaagaatta
     5881 gttacgttaa atacggaacg caagatagac ttgaaaaacg gtaacaaaag aagtagtata
     5941 ttaaggtgtg atgtgctact tgacatttat tctgctgagc gaactttctg gattgtgtag
     6001 ccttatatct attcaggacg tatcgagcag attagtaaag gtgttcttga tgattttcat
     6061 cttgaacacc tttgttttta gcaattcgca aaaaacgaaa agtaggagaa ttgataaatg
     6121 aaaaaaagat ttttagcact tgccatcgta ctaggcactg gcttattgtc aggctgtacg
     6181 aatgccggtg aaaaaacagc cgttagttat aaaggcggta caatcagcga acaagaagta
     6241 atggatagct tgaaaaaaat gcaaggagca gattccgctg tgcaacagtt gatcgtttat
     6301 caagtatttg aagataaata tggagatgac gtatcaacca aagaaatcga ttctcaatat
     6361 gatcaaacaa agaaacaatt gggtgattca tttgattcac aattgaaatc tgcaggttac
     6421 acagaacaaa cattcaaaga cagtatcaaa caaagtctag cctttcaaga aggtctaaag
     6481 aaacatatca aattgaccga tgaagattta aaaacggctt gggaatcttt ccatccagaa
     6541 gtggaagctc aaattatcca agtagcaagt gaagatgatg caaaagatgt gaagaaagct
     6601 gctgataagg gagacgattt ctctaaatta gccaaagaca agtcaactga tactacgaca
     6661 aaagaagatg gcggaaaagt gaaatttgat tccactacta caacggttcc ggcagaagtc
     6721 aaagaagcag cctttaagtt gaaagatggt caagtttctg atgtgatcac ttctaccaat
     6781 gcttcaactt atactacgga atattatgtg gttaaaatgg tgaaaaacca aaacaaaggc
     6841 aacgacatgg ataaatacaa aaaagaattg aaagaaattg caacagacac aaaattaagt
     6901 gacagtacct tccagaataa agtaattggc gaagtattga aagacgctaa tgtaaaaatc
     6961 aaagacaaag actttgaaaa tgttttgagt acattcactt cagattctag tacagcaagt
     7021 agtaaataag cggtataaat aaaaaatatg aactatttaa atcaataaaa ctgttcatct
     7081 aaatcttttt attagaacta aatcaacagt agtttaatta ctacttgatt tagttctttt
     7141 tattttaaaa atttttttta cttagaatcc agcgacccta gatatttttc tgtataatct
     7201 taaataaaga aaacaatatc aaaaaatgca cgtagaagta gataaatgga gtggttttgc
     7261 aaatttcttt tatttctaga agttattcac gtatacggat ttgtttttct cacaaataaa
     7321 aaggcaggaa gtacaatgga tcatttgtat ataaaatata ttcatcaatt ttccgttttg
     7381 gtgttaggca cactaattat agcggggatt gtcgcattta ttattcaagg ccgtgacata
     7441 ttaacagtac atcaagagtt tttcagttat gcatttggta ttcctctgat cgctataagg
     7501 tctgataaca aaaagtggaa gaaacgctat tacattctct tgctattttt gtgtttttat
     7561 ctgccaccta agatttttac tttattaggt atacaatgcc tatcattttc ttttatttcc
     7621 tatatcattg ttttaatttt cctcagtttc atagtatgga tgttttatct tgtcaaaaaa
     7681 taaatgaatg tttcatgaac agtacttcat cacagcctaa tttttttctt tatgctatcg
     7741 ttatattaaa gaaatcaatc agaagggaaa gaaagtgatg aaaaaagttt tattagtggt
     7801 aaaccccagt tcaggcggtg aacaagcaaa ggaatttgag caattagcga tagcaaaatt
     7861 ggaatcggtg tttgatgaag tggtcgtctt gcatacaaaa aaagcagggg atgcaaaaaa
     7921 ctttactcgc gaagcggcta cggaagggta tcatagtgtg tttgtcatgg gcggagatgg
     7981 aacagtcaac gaaggaatca gtggaatcgc tgaacaagaa catcggccaa atttcggatt
     8041 tttcccattg ggtacggtga atgacttggc tcgagcatta gggataccgc tagaacctga
     8101 ggaagcgatc aatcattttt cgattgaatc agtcaagcca ttggatattg gaaaaataaa
     8161 tgacgactat tttatgaatg tcgtcgcaat tggctctatc cctgaatcaa tcaatgatgt
     8221 agatgcggag aagaagacga aatttggaaa actagcttat ttcatgtcag gaatcaaaca
     8281 gttagcttcc acacaatcct actcgtttca cgtagaagtt gatggaaaga aggaagaaat
     8341 tgagagcagt accttgctga taggactaac taactcagtc ggcggttttg aaacattact
     8401 tccaaatgca aaagtggatg atggtaagct ccatttggtt tatttaaaag attcttcttt
     8461 gatcgatacg cttaaaacat tgccagacct attaaaagga gtagatgaat ctacgaaaaa
     8521 tctagcctac cttacttgtg agcaattagt tgtgtcatta gctgatgagg aagaattgac
     8581 gacgaatgtt gatggagatg aaggggataa attacctgtg acaatcaaag tattgccctc
     8641 acatttacag gtttattgtt aaaatggagg ttgtgaccga ggagttggcg ggagaacacc
     8701 gcttgttcgg gataagggac tgtgacaaga cttttgtcac agcctttttt ttgtacaaaa
     8761 aagaaattta cacctaatag aaaaattttt ccataaaaat acatatttta aatatgaaag
     8821 tacaattgga aatagattaa aacgataaaa taaaattgtc aaaacgtcag agaggatggt
     8881 ggagaatgaa tcaatgtgta aaaaagctga ttgcgtcgat tttgtttgtc ttccctctct
     8941 tgatggtgac atccgttttt gccgatgtag agaaaacaaa cgaatcaacg caatcagaga
     9001 atcaaagttt tgacaagact actttttcag aagaatcgat tacttctgaa actacagaat
     9061 ctcaaagtga ggcttctact aataaaaggc aagaagaaaa taagacaagt gagacctcag
     9121 aagtagctga acaaacgtct gatagcagtg aagcggcact ggcaagagct gtttcagcgg
     9181 atgggtacag cgaagctgcc acaacagaag aactggcgca gcttctcgca gacgaatcag
     9241 taactaagat ccgactgatt cagcctttga ctttagacag ggaattagag atcaaacgag
     9301 atatcgttat tgattttggt ggattcgctc acaatttcgg cacacaccat atttatatca
     9361 atgagatacc tcagtcgatc gagttccaga attttaaagg aacagctgca catccaggtg
     9421 gattgacacc aaatatcgat ggaaatgcca ttatcattgc ttatatgacc gatttttggg
     9481 gaagcagata tcattttacg ggagaaataa aatttaccgg gacacttgat ttgtatgatg
     9541 ggtccaaatt aggactgatt tacgctcctc gtgcaactgt cacattagat ggtgtttccg
     9601 gtgtgttgga tgtccaacca gtgaaggaag gactgaatgc cgctccgggt aaagcctatt
     9661 tttgtagaag ctatcaattg aatgtgatca atggtagtca actttatgga ccgtatctag
     9721 ggaaattcta cggttttcta gattcaggag atgagactgg tagtggaaaa acagcgccag
     9781 gaatccatat cttaagcggt tcaaaggtct ctcttgatta tgatcgggca gatggcgtta
     9841 gcgatgaagg agaggcagtc gatacgctac ctggtaatgt catgtttgaa gtcagtggtt
     9901 cgggaagcga attttcggta aatacaaaga tcaatattac caatgacaac aatcgcggaa
     9961 tcatccaaat gagaggttca ggatcgaaag tcactgtatc gaatggtggg aaaatcacca
    10021 tcaatacagc aactacgggc ggatttcgct tgcaaggaga ttctagtcaa atccatgtat
    10081 caagtggtgg acaaatctcg gtcaaaatgg ctggagatga cgatcaaccc gggaacaatg
    10141 ggatgcgttt tgtaggaaaa ggcctttctt tgatcgtaga aggagccgga tcagttatcg
    10201 acatcgataa gcagagtggg cgttccagcg cagttcgttt tgagaatggt acgcaaaagc
    10261 tgacagtaac caatggaggc tcattgaggg taagaaatac aggtgatggc aaatcttacg
    10321 tgaacagact caatcaagga aatcaagcga tccaatttta tgacgcttct ggttttctag
    10381 aaagtccagg ttcagctgat tttacagtca ctggagaaaa atcaaatatc gatatacgcg
    10441 cagattccgg agcaactgta gatacaactg gagatattga tttaaatttt acggcaggtg
    10501 aaaaaactta tttagtcatg gtcgggaaaa caggggaaga taagaccggc atcatcagtg
    10561 gggatgtgtt gaatgtggac ttagaatctc ctacctattt tgatttccaa aacaaacgga
    10621 ttggaagcga gaccaatggc ataggcggct gggtattcca aggaaatgga gattcttctt
    10681 taaagatcgc ccattcagaa atcgctttat ggcgaaaaac aggcgcaaca ttcgataact
    10741 ttgaaaaaga cccggattat ttttctgata tgacagactt atcgatcaat gggaccgact
    10801 taggcaattt cgtatcaagc cctagtgcag gtttaacaaa tgaatttgct cagacaggtg
    10861 ccggaatgaa aaattataat cgaatcagtg ccaataatca gctcccgatg attgaatcct
    10921 taagagtgcc aacgaatgca gataaaaaaa tctatgggca tgtcgttgtt cccgaaggtg
    10981 tcgaggcaaa gccaagagat gcttggacag gcgaagtaga agtgacttta cgtattactt
    11041 acgcagatac gacgaaatca tctgtagaaa ttactgctct cacgcaagga caaacagatg
    11101 aaaatgaagg ggcaggatat aacccatggg gagaaggcga acaaggcggc ttgttcgaag
    11161 ttcaagtacc caatgatgag agattaaaaa cggaggatca agtgcaagtt gttgctgcta
    11221 aaaaagtaag aggtggccaa gaagtgacag aactggactc tgccaaaacg acagtcgatg
    11281 tgattcctcc tgaacctgct aatttaagta ccacattgat caatgcagct tctcgcacga
    11341 tcacaggtac aggtgaagca ggagccttgg tttcattgaa aaaaaatcaa gctgttttgt
    11401 cagaaacaac tattgatgat acaggggaat ttgttctttc tatcccagaa aatcaacttt
    11461 ttgcaggaga tgtattagaa attgtattaa acgaccaagc aggagaagct actgctaagg
    11521 gtgtgcagaa caagccaagt accaacgatg aaattgggaa tcacaatcca agtgatacgc
    11581 cagttgttta tcatgatgcc ccgccttttg cacctgctac gaagatcgta gtagaaggtg
    11641 gtctctcatt tcttgcccct tccaaactcg attttggtca gatcaaagca actggtttga
    11701 cacaagaagc atttggccaa atggatatac aggaaaaatt atggatttat gatcagcgag
    11761 aaacgaaaaa tccgtggatc ttaacgataa aattatctca gcctttcaca aatgatgcag
    11821 gattttctat gcaggatgtc ctatatttca agaaaggaga ggagtatcaa ttagtgaatg
    11881 aagaagaact ggaaattgtc agaggacaaa attcaacgaa tgtgcaagca gattattcag
    11941 aatatttaaa taacggtcgg gcgtttaaac ttgtcttaac caaagaaaat cagatagctg
    12001 gtaattactc agcagagata acatggacat tagcagatgc gccgacagaa taaagatcaa
    12061 ggaggatgat tgatgaatcg aatatggaat aaaagtcatc gaaacaactg ctctttactt
    12121 atgatccttc tccttggatt catcctttgg cagacacctg tccatgcctc cgaattcaat
    12181 tttgcggtag attttgaatt accggctaat cagattgata agaacaaaac atatataaat
    12241 gtaaggatgg agcctgggaa aacacaggaa atcccctaca cggtctttaa ccgaacagat
    12301 aaatcagtaa cagttgagac attgatccac tccgctacga cgaattcaaa cggtgtgatc
    12361 gaatatggag aatcaaaaag taaatttgat gaaacattaa gttatgatat gaaaaactta
    12421 gcaaaaacag tgggagaaat aactgttcct cctcaaagtg cggtcactga gacactttca
    12481 ctgaaagcgc cagaaaaaga gctggctggt gtggtcgcag gagggatcac gtttcaagaa
    12541 aaaaaagaga acgtttctaa caaaagtaca gttggtgtcg tcaatcagtt ttctcaagtg
    12601 aaagcaatct tgatttgaaa tagcgaaaaa gagtcttccc cagatttgaa attaaaaaaa
    12661 gcttatgcgg atcaaagcga tggaagaaat gtgattaagg cgaatcttca aaatccaaat
    12721 gcttcttata tgttcggctt gactacatcg gttgaaatct acaaaaaaga aacaaaagag
    12781 ccttaccttt caataaaaaa agaaaaaata gatgtagcac ccaatacaaa ttttgattta
    12841 cttgtcccat tagaaggaaa aaaattagct ccgggacatt atcaagcggt tgtgaagggt
    12901 gcttggaaag aaaaacgctg gaatctccat accgattttg aaataactga tgatgcagcg
    12961 gaccaattga atgaaaagga tattgattta aaaaatcagc cagaaacatt tgactggcga
    13021 tggctgatac ttgttggagt cattcttatt ggcttatttg ctggattatt tattttaaaa
    13081 gaaagaagga aaaagaaatg aaaaaaacaa ttgcattaag taccgttcta gctggaagtc
    13141 tattattatg tacacaaagt gtttttgctg agaataagag ctcagccact acagaaggaa
    13201 cgatttctgt gaccaaacca gatgctgcaa atcctgaaga cgttgttcca gttcctcctg
    13261 ttgatccaaa tgatcctgat actcctttta cacctgacga accaaatcag ggaacatccg
    13321 gattactgac gattgaccaa gctccagatc ttgattttgg aacaatcaag ttaggtacag
    13381 caaaaacagt atatgctgca ttacaaagcg gaacggacag tactggagca caaacacaag
    13441 tacagaattt cgtacaagta acggataaaa gcggaaatta tgccggttgg aaattatcag
    13501 tcaaacgaac agaatttatt ggtacaacat ctgatacgaa taaactaact ggttcaaagg
    13561 tgaaattcaa aaacgccgtg attcgaaata gcgcaactaa tagcagtgga atgccatcta
    13621 ccgtttctgg aactggcgct gaaggaatcg aaatcccagc agatcaggca gtagatctag
    13681 taactgcagg agcaaatgaa gggattggca catggattta ttcattagga gagaatgtgg
    13741 aacaagggag acaatcagtg gaattatttg ttcctgtatc caattatgct gtagacaact
    13801 acgtttcaac attcacatgg tcgttaacag atgctcctac agaagaggaa gcgtaaaatc
    13861 atatcaacga tttcagggag tggagccgat tcaaggccca ctccctgaaa tctgctatgc
    13921 ttacgtctca gctcttattc atcaactcga agcagttttt tggaagtcat ggaaacgttc
    13981 ctttgtcttt tcaaagatct ttcgaccaac gagtaaggag tcaccgttga aaaattctaa
    14041 cacataatga gcagaatcaa ttttctgcaa ttgcgataag ttgatacagt aagattttaa
    14101 aagtaaaaag taagcatact tttctaactc tgtcttcaat tgttttaaag aagtattcac
    14161 taatttatct tctgtagttg ttttgaccat aacttggttt ttcaaacctt tgatactttc
    14221 aatatataaa atttcccgag gatacaggac gagacttcca cctttttgat caataaagat
    14281 tttatcgaca tttacgatct gctgttcttt ccaattgatc tgttctagca cttgacgaat
    14341 ttgttcagat aaattcctgt cttttctgat atatccaatc ggaaggattt tccgattgat
    14401 gatctctact gcaaaattcg tataagcggt gaaaaaaaca atattactat gtgcattttt
    14461 tttacgtatt tgttcggcga catcaatacc tgtgagtgat tgcttcaagt cgatatcgat
    14521 aaagaaaaca tcgtgattat caatagtgcc caactggatc aactgattga tttgagagat
    14581 agaagtgagc tgcacgatat tctcgatatg atgattgacc aattctctga tgatccattc
    14641 gctgtgaaac tggttatctt ctatgataaa atactttttc atatgcaggc tcctattaaa
    14701 aaaatatgtg tacattttga aacaaaacaa tggaggaata agatggtcgc aacagtgaca
    14761 ctttatttga cctttacttt gattttagat ttaggtatct atttattctt ttcgaaacaa
    14821 ctgctagtaa aaaacagctt tggttgtata gctttgatgg tggtgacact tttacttcct
    14881 atccctaaag tgagctttcc ttacctagtt ggcttattat tgatgtttct tttttatttt
    14941 actacgaaaa acctgtttat cagctgtttg atcagtaatc ttttttttgc tatcactgga
    15001 ctcagctttt ttctggtttt tgatctgatg gattatttag gaaagagaca tcttggaaca
    15061 gcaagttttt ttgcactact tatcgcattt attttctatc tattagtaaa aagtatagac
    15121 aaacagacga gatttctcaa cttgcttttt acttatccaa ctgaacacta ttttggaggt
    15181 atttgtattt tattaagctg gggcgtgatt tacttggtat tgggaagtgt tcacttccgc
    15241 tcatctaatt atctgcttct atctcttttg tcgatagtca tcagtttttt ttctttgttt
    15301 ttttccatta tgttgatcag tttccaagta aaagaaagac aacatcagga atcgcttcaa
    15361 ctgtatcaaa taaatgaaga atattaccat catctagagg aattcaaaca tgattataaa
    15421 agtctactgt tttcattaaa aattactctt caggaaaata aaaaagaaac gttggaattt
    15481 ttagaaatat tagaagatca ttctgcagct atttttgaaa aaccgcaaat acaacagcta
    15541 acaaatattg tatccccagc tgtcaggggt gtctttttaa aatttctaga aaaagcagaa
    15601 tcggagaaaa tcccgatcca agtagttatt cctgtcgaag tgaaaacaat cccgattgat
    15661 ctggttgttt ttattcgctg tttatctatt tttatttcta acgcattcga tcatcgtgta
    15721 gccgatcaga gtccgattac gatcaatctg ctggaagaaa aagagggatc acgttttagt
    15781 atagcgaaca aggcaaatcc aacgaatttg acgctatcgg agatggtcaa aagaggaaaa
    15841 acctctaaaa aaaatggcgg gttaggtcta tacagtgcga aaaaaatgtt ggatgtttac
    15901 gaaaatgcag aactaaaaat cgaattttca aaacagaatc actatttttt tgtagaaatg
    15961 tatctggcat ctagaaagag caaatcagcc tactgactta ctgtcaaagg ataagtttga
    16021 ggaaagtcta cacggagata gagacaacaa cacagcatat tcaaaagaaa cagaagcgtg
    16081 atgaccgttg tcacccttct gtttcttttg ctatgtagtt aaacaaagta gttgacactt
    16141 ccgctactta gtattagtac gaaagtatac ttcatgatac taagtagata agaagtgaaa
    16201 agcggaaccc acgatcgtca aaatgctaac tgctttgctt acaccagata aagggaagag
    16261 caatatcgct tattttaggc gtggagaagc aagcaaatcc ctacgatcac aaaaaaaatg
    16321 ctgacccagc agttaagaga gttagaacaa gatgaaagta tccatcgaaa agcctataac
    16381 caagtcccgc ctaaagtaga gtactcgctc accgaagaag gaaaatcatt aagagagatt
    16441 ctagtagcgt tgtctgtttg ggaagaagaa cgtatcgaaa aacaaaaagc ctcaagggaa
    16501 gaagacacgc tactgcatac acatggtggc tacctaaatt attgattttc tatgacattt
    16561 attttaaata aaagaaaaca ggagtttttt taaaagcatc atctattttt tatgtaaaaa
    16621 aatggtatga ttaatcaagt aagaagtgat tggaagaagg caagcaaatg aatagtatgg
    16681 aaaaagtcaa ccagtttaga gatgaacgta attggcgaca atttcataac gaaaaagatt
    16741 tggctatctc tatttctttg gaagcatctg aattgttaga actttttcaa tggaaaacgc
    16801 cggaagaagt aaaagaaaca caattagaac gaatcaaaga agaactggca gatgtattga
    16861 tctattcata catgatggca gacaaccttc acctggatct agatgagatc attgaagaaa
    16921 aattgatcaa aaataatttg aaatatcctg tagaaaaaag cgcaggtgta cgaaaaaaat
    16981 acacagagtt gtagaaatgt gtcattcggc tgttggaaag agcaaaagaa aaacagtaaa
    17041 atgagtctgg agggagagaa aatgaaaaaa aaaggaaaag caaaaagatg gctagtcaat
    17101 atcctcttgt tcctcttgtt attagtagga ctagctttga ttttcaatga acagatcaag
    17161 gattattttg tcagagagac aggagataaa tatgctatag cgaatgtaac aaaagaagat
    17221 ctgaaaaaga ataacgataa agatgtcagt tttgactttg atgcggtaga accaatgacg
    17281 acagaagggg tcatgaggtc gcaaatgaga ggaacagatc taccagtgat tgctagtatc
    17341 gcagtccctt cagtttcaat caatttaccg gttttcaaag gcttggataa tacgtcacta
    17401 ctttacggag caggaacatt atctccagat caagaaatgg gaaaaggaaa ttatgcctta
    17461 gcaagccatc gtgcaacgaa ccctgaattg ctgtttacac cgctagaaaa tttagagatg
    17521 ggtgccaaga tctatttgac agatttagaa aatgtgtata catacaaaac attttttaaa
    17581 gaaaaagttg ctcctacaga tacccaatta ctgaatgaag tcgagggaaa agaaattgtc
    17641 acactaatta cttgtggcga catggacgcc gtcacgcgac tagttgtaca aggagaactg
    17701 gaaagtgtta catcgataaa agatgcgaca gatgatatga gatctgcttt caatctagag
    17761 acaaaaacat tttaggatac tgggacataa gtcgttacag attaaaaaca gccaaaggat
    17821 atgaatcgat aaggaaaaac caatttgctg aaaaaaagag aaacgcaaag aggtgcatct
    17881 ctgatttggt tgttttctag atagacttgt gttccagttt ttttattttt tatatgaaaa
    17941 ggcttgcata aataaaaatt cgatggtatg atgattttga ataagaaatg gattgttact
    18001 gtacggcagg caaaaccaga attcagctaa gaaacagtag gcgaattcta ggttttttta
    18061 tttttcttga gagggcgaag caaaatgaaa attgataaaa ttttgaataa caatgtcgtc
    18121 atttcgaaaa atggattcgg ggaagaagtc gtttgtatgg gaaaaggact agcttttcaa
    18181 aaaaagattg gggacgagat ttcgccagaa g
//
LOCUS       DAYLZO010000058        18173 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.059, whole genome shotgun sequence.
ACCESSION   DAYLZO010000058 DAYLZO010000000
VERSION     DAYLZO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.059"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(129..585)
                     /locus_tag="ACGVJG_001742"
                     /pseudo
     CDS             complement(129..585)
                     /locus_tag="ACGVJG_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            750..3112
                     /locus_tag="ACGVJG_001743"
                     /pseudo
     CDS             750..3112
                     /locus_tag="ACGVJG_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3480..4403
                     /gene="truB"
                     /locus_tag="ACGVJG_001744"
     CDS             3480..4403
                     /gene="truB"
                     /locus_tag="ACGVJG_001744"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFJ6557682.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4407..5348
                     /gene="ribF"
                     /locus_tag="ACGVJG_001745"
     CDS             4407..5348
                     /gene="ribF"
                     /locus_tag="ACGVJG_001745"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFJ6557683.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5700..6485
                     /locus_tag="ACGVJG_001746"
     CDS             5700..6485
                     /locus_tag="ACGVJG_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFJ6557684.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6498..7349
                     /locus_tag="ACGVJG_001747"
     CDS             6498..7349
                     /locus_tag="ACGVJG_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFJ6557685.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7473..7925)
                     /locus_tag="ACGVJG_001748"
     CDS             complement(7473..7925)
                     /locus_tag="ACGVJG_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557686.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8390..8758)
                     /locus_tag="ACGVJG_001749"
     CDS             complement(8390..8758)
                     /locus_tag="ACGVJG_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFJ6557687.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8908..9390
                     /locus_tag="ACGVJG_001750"
     CDS             8908..9390
                     /locus_tag="ACGVJG_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFJ6557688.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9427..10437
                     /locus_tag="ACGVJG_001751"
     CDS             9427..10437
                     /locus_tag="ACGVJG_001751"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFJ6557689.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10552..11265)
                     /locus_tag="ACGVJG_001752"
     CDS             complement(10552..11265)
                     /locus_tag="ACGVJG_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557690.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11438..12045)
                     /locus_tag="ACGVJG_001753"
                     /pseudo
     CDS             complement(11438..12045)
                     /locus_tag="ACGVJG_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            12529..12756
                     /locus_tag="ACGVJG_001754"
     CDS             12529..12756
                     /locus_tag="ACGVJG_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFJ6557691.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12772..13938
                     /gene="hemW"
                     /locus_tag="ACGVJG_001755"
     CDS             12772..13938
                     /gene="hemW"
                     /locus_tag="ACGVJG_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFJ6557692.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14172..15215
                     /gene="hrcA"
                     /locus_tag="ACGVJG_001756"
     CDS             14172..15215
                     /gene="hrcA"
                     /locus_tag="ACGVJG_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFJ6557693.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15309..15872
                     /gene="grpE"
                     /locus_tag="ACGVJG_001757"
     CDS             15309..15872
                     /gene="grpE"
                     /locus_tag="ACGVJG_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFJ6557694.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15923..17752
                     /gene="dnaK"
                     /locus_tag="ACGVJG_001758"
     CDS             15923..17752
                     /gene="dnaK"
                     /locus_tag="ACGVJG_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFJ6557695.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17903..>18173
                     /locus_tag="ACGVJG_001759"
     CDS             17903..>18173
                     /locus_tag="ACGVJG_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFJ6557696.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGF"
BASE COUNT         6184 a         3070 c         3783 g         5136 t
ORIGIN      
        1 caggggttgc gggatttttt tgttttcatt aagacgtatt acctaagagt tccatgactt
       61 tagtcttgcg gcgtgtcagc taaccaaaaa agagaaaaag aaaagtaaaa aattcgactt
      121 tcttttttct agtagtatat aaatttttgc atatagataa agatcaagat accgataacg
      181 gaagctagat tgtaatctat tttagtgaca gtcaggtata gccaagtaac tagaagatca
      241 gtggaacctg caccataaaa gtttgagttt gtcggtccgt tgacagttaa aagaaaaatc
      301 acgttgaagt tattgatatt tccgattacg ccgcttcgat ttggtcacga tccaaatttt
      361 ggataatccc tgtagaaatt agcatagtag ctgggatacc aattcacata ttgacaaaga
      421 taacagagac ttttacataa aacggatcaa tcaggaaagg aattgctcga tccagcccca
      481 gttttccagc aatgtgttaa ctggcccagc gtcattcagc aaattatgca tgatgaggag
      541 cgaaatgaac tgcggaacag ccattgtgat aacgacaagt tttttggatc aatgtaaaaa
      601 acgtcagcga aaggaaaaat ttcgtggttt tttatacctg ttcatttggc tagatataaa
      661 atccaaacaa atgaacagaa gtgcttatta ggtttcaatt gggagtatct gcgctagaat
      721 aagagtaata atgaaaagga gtgttatgta tggattattc tagtaaagaa tactttgata
      781 aaatgacggc ttggtggcgt gctgctaatt atctttctgt tgggcaactt tatcttaaag
      841 ataacccctt actgagacgt acactgaaac ccgaagatgt caaaaaacat ccaatcggtc
      901 attggggaac gattcctggc caaaatttta tttatgtcca tttgaaccga gtcatcaata
      961 aatatgattt gaacatgttt tatattgaag ggccaggaca cggcggacaa gtcatggtat
     1021 cgaatgctta tttagatgga agttacacag agatctatcc agaagtaacg gaagatgaga
     1081 caggaatgca aaaattattc aaacgatttt catttcctgg aggcattgct tctcatgcag
     1141 caccagaaac accaggttct atccatgaag gcggagagct tggatactct ctttcacatg
     1201 cagtcggagc tgtattagac aatcctgaag taatctctgc tgtcgtgatt ggtgatggtg
     1261 aagcagaaac cggtccttta gctggaagct ggttctcaaa tgtctttatc aatccagtga
     1321 tagatggagc tgttttaccg atccttcatt taaatggtgc aaaaatagcc aacccaacga
     1381 tcttggcacg taaatcagat ggagaattag caaattactt taacggactt ggctgggaac
     1441 ctttctttat tgaaggaaat gattcagaaa aattaaatcc tgttatggct gaaaaaatgg
     1501 atcaagcaat cgagaaaatt aaatccatcc aaaaagaagc tcgcctgaag acagcaacag
     1561 atgtcgtgat gccaaaatgg ccagtattga tcgtacgcac accaaaagga tggaccggtc
     1621 caaaagaatg ggacggggaa ccaattgaag gaaccttccg agcacaccaa gtaccgattc
     1681 ctgtagatca agaacatatg gatcatgcag atgctttact ccgttggctg aaatcctatg
     1741 aaccagaaaa attatttgat gcacaaggac gtattttaga agaaatcaga gaaatcgcgc
     1801 ctactggtga ccaaagaatg gcaaaaaatc ctatcaccaa tggtggaatc gatccaagac
     1861 cgcttattat gccagattgg aaaaaatata ctttgcaatt tgaaaaacca ggctccgtta
     1921 aagcagaaga tatgaccgaa cttggtaaat tcgttcgcga gatcattgag aaaaatccag
     1981 aaaatttccg tatatttgga ccagatgaaa caaaatcaaa ccgattgaat caagttttca
     2041 aaacgacaaa tcgccaatgg atggaaaaaa tcgaaccaga aaatgatgaa tggctttcgc
     2101 catctggtcg cgtcatcgac agtcaattat cggaacacca agatgaaggg ttcttagaag
     2161 ggtacgttct gactggacgg catggttttt tgcaagttac gaatcattct tgcgagtggt
     2221 tgactctatg ttgactcagc acttcaaatg gatgcggaaa tcacatgacc tttcttggcg
     2281 aaatgattat ccttcgctaa atctcattgc aagctcaaca gtcttccagc aagatcacaa
     2341 tggatatagc caccaagatc caggaatatt gacacatttg gcagaaaaga aggcggagtt
     2401 catccgtgaa tatctgcctg cggatgcaaa tacattgctc gcagtcatgg ataaagcatt
     2461 ccgctcgtca gaaaaaatca atttgatcat ctcttccaaa catccgcgtg ctcaatttta
     2521 ctcagcagaa gaagcagccg tcttagtgaa tgaaggcttg aaaatcattg attgggcttc
     2581 gacagcaaaa gaggaggaac ctgaattggt cattgctgct gcaggaacgg aatctaactt
     2641 agaagcatta gcagctgtaa ctttgctgct ggaagaattc ccaaaactaa aaattcggtt
     2701 catcaatgtc gtagatttat tgaaattacg tcatccaagt caagatccgc gtgggttgag
     2761 cgatgaagaa tttgatcaat attttacaaa agacaaaccg atcttatttg ctttccacgg
     2821 ttatgaaaca ttggtccgca cgattttctt tgatcgtcat aaccatcatt taatgattca
     2881 tggctacaaa gaaaatggag atatcacgac accatttgac atgcgtgtag taaatgaact
     2941 tgatcgttat catttggcaa aagacgcagc tttaaaaatc aaaggaagtc aagcagaaga
     3001 ttttgcgaaa aaaatggatc agaaattaca agaacatcaa aactatattc gtgaaaatgg
     3061 tattgatctg ccagaagtat tggattggaa atggaaaaat cttgaccaat aaaatgataa
     3121 aatcgatccc gcaaaataga attttttaca ttgattaaga aactgaaaaa ataaataaaa
     3181 aaaacggaaa aaagactgga agaatggcca gtcttttttt tcgtttttgg gaagtgaaaa
     3241 ccaaactatt tacctgtaag ccacccataa atccaatcaa atggtgcaaa gaaaaaatct
     3301 tccattctat tccctccttt aaaaaagcat aacatgattt ctatttacgt gagcatgaat
     3361 tttgttacgt ttttattgcc gaaaaataaa aaccgttcat tatgatgtca aattcgtcag
     3421 taattgatgc aatgtgttat aataattcag ttaaatcaaa aatgaaatgg agaaaagaca
     3481 tggacgggtt attaccttta tggaaagaac gcggaatgac aagtcatgat tgcgtattca
     3541 aattaagaaa aatcttacat acgaaaaaaa tcggacatgg gggtacgctt gacccagacg
     3601 tagacggtgt gttgcccatt tgtattggaa aagccacaaa agttatcgaa tatttgacag
     3661 attctgggaa aatctatgta ggcgagataa ctctaggttt cagtacaacg acggaagatc
     3721 gatccggaga acgggtagaa acaaaaccag tagatatgtc tttatctgaa aaaaaaatag
     3781 atgaagtgat ggcttcattt attggtgaga tcacccagat accaccaatg tactcagcgg
     3841 taaaagtcaa cggtaagcgg ttatatgaat atgcccgcaa taatgaaaca gtggagcgtc
     3901 ctgttagaaa ggctcagatt taccgtttta tccggacaag tgaattgaca agagacgaag
     3961 tgacaggaac catttcttgg cgcttcgaag tagaatgcgg gaaaggaacc tatgtccgca
     4021 cattagctgt agatacaggc aagaaacttg gttaccctgc gcacatgtct gatctgaccc
     4081 gaacagcaag tgccggtatg tcccagaagg atgctctgac attagaagaa gtagctcaag
     4141 ctgtgaaaaa tgggacaatc gatgattgtt tgctgccaat agaaaaagga gtggaacggt
     4201 tcaaacgagt agatattact gatgaagtat ggcagaaagt aaaaaacggt atgagacttg
     4261 attatactgt atttgcgctc ccagaaatgc cgtcagaaga aattgctttg ttttataaag
     4321 gaaaagttgt cagtatttat cagccaaacc cgaaagaaaa aaacaaatta aaacctagta
     4381 aagtattgag aaatgaggaa taacgaatga atatcatcaa gatcagacat ccttaccaat
     4441 cagaacaaat tccagaagac gatgtcgttt tagtgctggg atttttcgac ggcgtgcata
     4501 tcggacacca gaaagtcata aagacaggta tggaaattgc acaaaaagaa ggattgaaat
     4561 tagcattgat gactttcaat cagcatccat ctattgtctt taaaaagatc gatcctagca
     4621 gcgtgaaata tttgactagc cttaagcaaa aagaagaaaa aatggccgct ttgggtatag
     4681 attatttata tgaagttgat tttacttctt cttttgctca tttagcaccg caggaatttg
     4741 ttgatcaata catcgtaggg ttacatgcaa aatatgctgt ttccggtttt gattatacgt
     4801 atggaccaaa agacattgca gatgtcgctc attttcctga atatgccaaa ggaagattca
     4861 aaatcgtcac agtttctaaa gaagaagaac aaggtgaaaa aatcagctct acccgtattc
     4921 gtggattact agacagcgga caagtaaaag aagcagcaaa actattaggt tgcacatatg
     4981 aggtagaagg ggtagttgtc catggcgatg cacgaggcag actactagga ttccctacag
     5041 caaatgtcaa agtaaagagc acggttcatt tgcctaaaga aggtgtttat gtgtcggaaa
     5101 taaaaattgg ggaaacatgg tatccagcca tgggttcaat tggacataat gatacattcg
     5161 gtgaaggacg ggagctgact gtagagttgt atattttgga ctttaatcaa gatatctatg
     5221 gtgaacatgt cgcggtacgc tggaatcaat ttttaagaga tcaagtgaaa ttttctagtg
     5281 tagaggaatt gattgaacaa ctcaaggagg atgaacaagc aacagctgaa tatttttctc
     5341 agcaataaaa cattatgcat aaaatctctt gtttatgtat aactagcaat tacaataaaa
     5401 tgggtgataa ataagcccag gaaaggggct gtgacaaaag tcttgtcaca gccttttttt
     5461 ccaaataaat ggtttttcag aagtagcttc ttcggaaata agccgaaatt tcttgaagct
     5521 gaccacttct gtcacaacct ctttctttat agaaataaaa ggacactagc aattcatgaa
     5581 aacgattgct agagcgtatc atttgcttta gactatatat cttggtgtac actcacttta
     5641 aggcggaaat aaaagttatc cgtaataaca ggtttaggta tgaaaaagga gtgggtacta
     5701 tgtacaagga attagaaaac aaagtcgcag tagtaactgg tggctcaaag gggattggaa
     5761 cagcgattgc tcaacgattc gggaaagaaa aaatgaatgt agttgtcaac tataactcgg
     5821 ataaagaagg agcagaaaaa gcagtcaaag caatcgaaga ggcaggcgga aaagcagtct
     5881 ctgtacaagc agatgtcagt tctgaagaag gaatacagcg tttacttgat acggctttag
     5941 aaaattttgg gacactggac gtttgggtta ataatgctgg gatggaaaat cagcatccaa
     6001 cccatgaact tagccttgaa gattgggaaa gagtgttaaa agtcaatttg actggcgttt
     6061 ttatcggaac gaaagcggct ttaaattact ttacagagaa taataaaaaa ggaaatatca
     6121 tcaatatgtc ttctgttcat gaacagatcc catggccaac gtttgctcat tacgctgctt
     6181 caaaaggcgg ggtaaaatta tttaccgaaa cggtagcaat ggagtatgca ccgaagggaa
     6241 tcagaatcaa tagtataggc cctggtgcta tccgtacacc tattaatgcg gcaaaatttg
     6301 atgatcctga acaaaaagaa ctcttggaaa gtatgatccc actaggacgc gtaggtgaac
     6361 caaaagaaat agcggcgaca gctgcatggt tggcttcaga cgaatccagt tatgttacag
     6421 gtattacact atttgcagat ggtgggatga ctttatatcc ttctttccaa ggtggaaaag
     6481 gataagggtg aataaaaatg ggtttattga ttgcattgat cccggcagtt ggctggggga
     6541 tccagccact gattttgaaa aagattggtg gcagaccaac aaatgagata ttaggaactg
     6601 gtattggagc ggtgcttgtc ggcttactcg ttcaattatt cgcttctcca gctggtattt
     6661 cagtgactac atttttgatt agtttacttt ctggtgcatt ttgggttatt ggacaaacag
     6721 gacagtatac atctttcaac ttgattggtg tttctaaaac gatgccaatt tcgactgcat
     6781 tacagttagt cggaacctcg ttgattggtg tcttcgcttt tggagaatgg tctggaagta
     6841 tggggaaaat cattggagca gcagcaatca ttcttcttgt atttggttct gctttaacgg
     6901 caatcagtga tgatcctgga aaaaaaggcg gtatcacaaa aggaattagt ctattagcat
     6961 caacaagtat tggttattgg gtatacagtg cattgcctaa actagttgat gcaagcggac
     7021 tttccatttt ctttccgcaa atgatcggtg tctttttagg ggcagtgatt tatgtgattt
     7081 tccgtaaacc aaccgcattt tctgaagaca agagttggaa agcaacgatc gttggagcca
     7141 tctttagtgt gtcagcattg gcttatattt tttcagcaaa tgaaaatggg gttgctactg
     7201 catatatcat tacacagcta aacgtagtta tctctacatt aggcggtatg tacatcctgc
     7261 atgaaaataa gacaccaaaa gaaaaaaaat taacgattat cggattattg ctgatcgtag
     7321 taggaagcat ggtcacggtc tttatttgag gataaagatg gtaaaatttc atttattaaa
     7381 aaaatatttg tttgaaccaa acagtaagta cttataaaaa aatagcgcta gaatttccaa
     7441 catctctgga atttctagcg ctttttaatt gtttaagata caagggaaat atctgtctgg
     7501 atcaataatt tttcattgat ccattgacta aagtttacga cataattgaa gttaccaccg
     7561 attagtatct ccgcatgatt gtctttataa agatcgtatt gtaaaagacg gtctttttta
     7621 tttgcgtaaa catcgttatg gtactggact aattgttctc ggaattgatt gattgctgta
     7681 aaatcacgat attgaaggtt gaaacgcatc gtgattggtg catttagcat gaatcgctgc
     7741 atttcttttg ctgcttcctc tgcttgatct ccgtatagga actcaaaaag attacgttca
     7801 gaaaatttcc attgcccgcc aacctttgtt ccttttagtt ttccttcatg aagataattt
     7861 ctgatagaac gtgtagtaac acctaattgt tcggctaatt catttactga gtaaaaatct
     7921 ttcaatgttc accctcctat gtgaatggtt ctactgactt agttgatatt tgcttaatca
     7981 cgaaatgtag aaagaacgat tttaattaag gtcattgtaa aaatcaagtc agaaaattct
     8041 aacttttatt tctctaaaga aattcgttct ctcctaaagt gatattatat ttaaatatac
     8101 ctgaaaataa tgggcagtat tatgaaagat cactgatatt ttctcgtatt ttttccttat
     8161 tcaaaagttt tgcaggaaaa ttgctgaaaa aataatttta ctaagtagaa aattcaaagc
     8221 cattcaatca gtgatttttt tcagcacaag aaagactcct tataaaaata gcagatactt
     8281 tgtcaattaa gtgtttaacc agggatgata aaaaggatag gattgaacaa aacctgtata
     8341 aggtgatgaa tagtttcctc gccagtaaaa ggtttattcg tataaagctt tatcgccaaa
     8401 atttatcatg acctttttta tcttcaatat taaatgaaat caattgttca atcaattccc
     8461 agttgattac acttttccaa ggaactttga tgacgttatc cgtatatacg tagcctgctt
     8521 cttggatatt tttttcaagg gcacgaatcg ctgctttttc cggtgcgata gaaaaatgat
     8581 ttttagcaaa actaaatgca ataataaagg taccatggtc agtaaacata ggttggttcc
     8641 attttacagt tgtgtctagc tggggatacg tttctgttat cttagcaaaa aggtctttga
     8701 tcttttgttg atgttctttt tctttgattt gattgatgat cggctgaaat ttttccattc
     8761 taaaacgcct ccattaagtg gtttgattta ttttaccaga aaaacaatag ttatgtataa
     8821 aaaacgaaca aaacacaagt aatgttgtaa ccgctatatt tatatgataa gatgtctcaa
     8881 ataataaaat agaaaagaga cataaaaatg attgacttgg caaagaaatt cttacacgaa
     8941 aatatatatt ttgagttgta tcatcatcga gccatttata caaatgaaga tgctttggta
     9001 ataaagaaag aacaaggatt tacaggtaca gagaccaaaa gcttattctt aaaagataaa
     9061 aaagataata actacgtata tctgactttt actacgaaac aaacggattt caaagcatta
     9121 gggaagatcg tcggtaaaag gctttctatt gtttcatcag aacgcatgga agaacaaacg
     9181 ggacaaaagc agggcgctgt ttctccgttt ggttatgaaa aacagattcc gattattatt
     9241 gatgaagagc tcttatcaaa tgaaaaactt gtttttgcac caggtagacc agatcaaacg
     9301 atggtcatcc aaacggttga cttaaacaaa atcatccagc tacttgagat gccgatattt
     9361 ttgatgccgg caaacgaaaa aaacaattaa ccaagtaaaa tagtgcatac ttggaggtga
     9421 agcaacatga taagttatcc attattagaa agaaatgcaa cgattggggt tactgcacca
     9481 tcctcaggcg tatcagagga acaccattcg ttactcgaat cagctttaaa gagaatggaa
     9541 gaaaaatctt tccaagtagt tactggaaag acgctttgga cgcaagataa ggaaaaatca
     9601 ggtccagcaa gaaaacgagc agatgaattc aatcaaatga tgcaagatga aaatatccaa
     9661 atgatctttc cgacttgggg aggagaatta ttgattgaga ttctcgattt tgtcgatttt
     9721 gatgctctac aaccaaaatg gatagtaggt tattcagata ttagtacgtt gctacttgct
     9781 gtcacattaa aaacagggat agccactgca catggaacta accttattga tttaagagga
     9841 gagtacttgg atgaaacgac agcaatgtgg gaaagtgtac tgaaaacaag agaaggagaa
     9901 tcgattctac agcaatcttc tgaaaagtat caaaaagtgt ggccccatga gcattcatca
     9961 tcttctgttt tccatctcac tgaaccaaca aattggcaat cagtcagcgg agatgcagta
    10021 aggatgaagg gacgattatt aggtggttgt attgatacga tcaggcattt aatcggtact
    10081 ccttatggag atgtccaatc ttttaaaaga aagtataatg atggaaagcc gatcttgtgg
    10141 tttctagaaa actgtgagct atcgtcagtt gatttaagaa gatcacttgt tcagatgaaa
    10201 ctggcaggct ggtttgatgg cagctcgggg atcatgttcg gtagaagtgc ggcagaggat
    10261 ttgttagatt ataaaatgaa ggatgtatac gaagatctgt caaaggaatt aaaactaccg
    10321 atcttgtatg atattgattg tggtcatgta ccgccacaac tgacattgat taatggtgcc
    10381 tatgcagaag tagcggtaga agatagaaaa gcgactgttt tgcaaacgtt tatttaaaaa
    10441 attttttcct gccttggagt ctatcaaaga ccaataaatc taaatagaaa agaaagtgaa
    10501 agaagctgac aaaatgctgt cagcttcttt ttgtataaca tattagaaag tctaattatc
    10561 tataactgtc acatcgatta actgtttcac gtcattggga tttcctgtag gaatagctac
    10621 tacagatgca ttatcaatga tttcttgagt tgtttctcta atgaacgctg gataagatgt
    10681 ttgatctgca ggcatttgcc aatgtgaagg aactggataa ctagtgattg tcccgtcatg
    10741 gttacttgaa tacactaaaa ctccctggta actgtatttc ccagtgagca taaccacatc
    10801 tttcggatag actgcactcc ctgcatcata aggggcgata ggtgttcctg caggataatg
    10861 aataacatgt aattcgaaat cacccgatgt atctctaaaa gtattacctc taatagctag
    10921 ccaaacacgt gcgtactcaa tttgtagatc agaatatcct gctaacggat ctattgactg
    10981 agaagactgt gaggaggttg tgtctattgt tgaagaagaa atatttgtct caactgtact
    11041 actagatgta gaagaagtat tgggctctga gactttactg cttgttgaag atgtattcgt
    11101 ttcagtagaa gctgatttct gagtacttga tttttcagct gaactggttt gtctgattga
    11161 acttttgtct gtggtcgcag atggatcact attttcttta ctaggagacg tacatcctac
    11221 aaggaataaa gagaccaaca ataaaataaa acctaacttt ttcatataaa atacctcatt
    11281 atttttttaa taccagtata gcataatcct gaatttttga atccgtaaaa ccttgtctat
    11341 gaatataaat agagtgtatc ttctttgata aatgtcagaa aaagtgtgac aaataatcat
    11401 cagtaaaatt acttgtcaca ctattgataa ggagtattta attgaagaga atgattagga
    11461 gaaaggatct ccgtactttt tatagtaatc atagaatcca agtttgtata gacgaatcgt
    11521 tttttctctt atttcttctg tttcaaaatg gactgcttga agaatcaagt ctgtgaattc
    11581 tataggagct gttccattcg cagtaatcaa attacgatct ttcacagctt gttgttctat
    11641 aaaatcttct ttattctgat aattttccga ttctaaccaa ggaaattgcg catttccggt
    11701 gtgtttgtaa ccttgcaata atccgctctg agctaaataa tcaactgccc cacaaatagc
    11761 agctactggt acgtctgttt ttaacaattt atcgattatt tgatataaat gatgattttt
    11821 tatgtcccat gaattgccac cgatcataat aaaaaggtca actttttcag gtatttgttc
    11881 gattaagtaa tcgatttttg tttttaatcc tccaatggaa gtgacctctg attggataga
    11941 agcagttttg ttttgccatt tctcattttg gtttagtgtg ctagtaatat aagcaccttc
    12001 ccaatcggca tattcctcta atagaaacgt aatcgcagtt ttcattttca agctctcctt
    12061 tactatctat aacaaggaca acagttttaa cctacagaca ttcattaaaa ttgggtagcg
    12121 acagcgaaaa caacaattct taagttttag acagagtata cagatagtca acttaattaa
    12181 atacgaacta aataaaaatg acaggtaatc atttttgttt accagcatta taacattttg
    12241 catacatgga tgacaaaaga accaattccc acttgcttga gtacaatatt aaaagaaact
    12301 ggaaggcaaa aagtaattat agaaacgaaa aataaatcat atcgggcact cagacatgaa
    12361 aacaacccta gagatatata actaggtaac cagtttaatg aaagaaaaaa tcatccaaga
    12421 agtcgattct tggatttttt agtggatttg ttcgccaaat acgtttagta tgcgtatatg
    12481 agaggttgta agcaaagcgt tgtggtctga gcacttaagg aggaatctgt gaaaatagct
    12541 tttcatattt ttgcagattt tgacttaatg ccgaaggtca gctttgcgat taccgtttat
    12601 cgagaggctg taaaagcacc gtgtagctcc gatgacaata agcaagcatt tttagagcta
    12661 ggtttccttg attctaaata tgcggcttat ttcaatgagt tggtgcttga acgcgatttt
    12721 aagaaggtcg aaaacaggca cgacttttcg acgtaaggag aaagaatttc aatgatagaa
    12781 acaataaatc ccgcagaaaa caaggtttct gcgtacattc atatcccatt ttgcgagcat
    12841 atctgctatt actgtgattt taataaagtt tttttagaag ggcagccagt agatgagtat
    12901 gtggaaatgc tgctgaatga aatgaaaata atgctagaaa gtcatccgac agcgaattta
    12961 gaaacacttt acgtaggggg cgggacacca acttctttat ctgctaaaca acttgatcga
    13021 ctgttatcag gtgctagaga gatcctgcct tttcaaaatg gtaatgagtt tactgtagaa
    13081 gcgaatccag gtgatttgac ccgtgaaaaa cttcaagtaa tgaaaaacta tggcgtcaat
    13141 cgtttatcca tgggagtgca gacatttgat aatcgcttat tgaaaaaaat tggacgtaaa
    13201 cacaccgcag aagatgtgta tcaaactatg caatttttag aagcagaaaa ttttaccaat
    13261 gtaagcatcg atttgattta tgcattaccg ggacaaacac tagaaggata tctggatact
    13321 ttggaaaaag ctttggcttt ggatctgcct cactattctt tgtactcatt gatcctcgaa
    13381 aacaaaacaa tgttcatgaa ctgggtacga caaggaagac tcgaactgcc tgatcaggaa
    13441 acggaaacca gaatgtttga agagacgatt gaagcaatgg aaaagcatgg acgtcaccaa
    13501 tatgaaataa gtaactttgg gctctccgga catgaatctc aacataacct catgtactgg
    13561 aacaatgccc actattttgg gtttggagct ggtgctagcg gttatcttgg agatattcgt
    13621 taccgcaaca aaggaccaat ccaacactat ttacgcccat tacgagcaaa tgaacgcccg
    13681 gtattggaag aagaaaaatt gacacgaaaa aatcaaatag aagaagaaat gttcttagga
    13741 ctaagaaaaa aaacagggat tactaaaaaa catttctatg atcgctatca aaaaacaatt
    13801 gaatcggtgt acggtgatgt gctaagggaa ttagagcaag aagggttact tgttaatgaa
    13861 gaagaccgtg tttatttgac accaagaggg acctttttgg ggaacaatgt gtttgagcgc
    13921 tttttgtttg aaaaatagtt taaagaatat ttgtttttta tctgaattta aataaaaaaa
    13981 gttcaaaaat acaataaagg actattgaaa cgttgatata actggattta catcaattat
    14041 ttagaattag cactcttgtg tttagagtgc taaaaaatgg agtctttctc ttgacaggag
    14101 aagggcttct tgctatattt ataagtggat tagcacttag aatatatgag tgctaatgag
    14161 aggtgaggaa gatgttaaca aagcgacaaa acgacatttt acgtctgatc atccaacact
    14221 atacgagtag tggcgtgccc gttggttcca aaacattgat gtctgaggga gtcaaggcca
    14281 gctcagcaac gattcgcaat gatatgaaag cattagaaga tgaaggattg ctgttgaaga
    14341 ctcactcttc ttctgggcgg attccatctg ctttaggcta tcgttattac gtggatcatt
    14401 tgttaaaacc tgctcgcgta gcagaagatg atttgctgct gattcgtcag tctctaggca
    14461 gagaatttca tgaaatcaat gagattatta agcaatctgc tgagatatta tctgagctga
    14521 caagctatac gactttttca ttgggacctg aaataaaaga ccgccgactg actggctttc
    14581 gtatcgtacc cttgaataat cgacagatta ttgctatcgt cgtaacagat atggggaatg
    14641 tagaaagcca agtgttcact cttcccgaat atatgggaag tcaggaacta gaaaaaatgg
    14701 tacagattgt aaacgaccgt ctagtcggag atcctctgat gacggtgtac cataaacttc
    14761 gtacggagat tccgatgatt cttcataaat atttccaaac gacagaaggg atgtctcagt
    14821 tatttgacac cgttcttggt catgtttttg aagataaggt ttatgtcagt ggacaaatga
    14881 atctcttgga ttatgagccc cgtcaagatc ttgatcagtt caaatcgatg ttttctttta
    14941 tgaaagactc agacggactt acacaaatga tcactccgat ggatagcaat attcatatca
    15001 agattggtgc tgaattaggc aatgatttat tgcagaatat gagtatgatt caagcaagtt
    15061 acgagataga tggtcatgga agaggtacaa tagccttgtt agggcctact agtatgcctt
    15121 attctcggat gttcggtcta gtcgatgttt atcgaagaga actagcagct aaacttgctg
    15181 attactaccg ttcgcttgac ttgtcaaact cgtaatgtga ataacgaagc tatttgcagc
    15241 tgatcgttct ctctttctta gtaagagcaa tcaaacagct gactgaataa tgaaaggaag
    15301 actgtatcgt gaaagaaaat caagaagaac tagataaaga aatgacagat gctcagccag
    15361 aaccggaaat cgatgtcgaa gctgcagaag attcgggtat ttcagaagct gaagcggaag
    15421 aattcgaaac agcgaaatta aaagctgagt tagaagaaat ggaagataaa tacttgcggg
    15481 ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa tgagcgtgag caattacaaa
    15541 aataccgttc acaagattta gccaaaaaat tacttccatc aattgacaac ctggaacgtg
    15601 cattagccac agaagtgtca gatgatcaag gggcaggttt gaaaaaaggc gtggaaatgg
    15661 tcttggaaag tttgcgtaac gcactggaag aagaaggaat tgaaaaaatc cctgctaaag
    15721 gagaagcctt tgatcctaat cttcatcaag cagtacaaac tgttccagcg actgaagata
    15781 caccagccga tacgattgta gaagtactac aagaaggcta caaattacat gatcgcgtgt
    15841 taagaccaac catggtcatt gttgcacaat aattgtgcgg atataaaact tacactttca
    15901 attaaagaag gagacgttaa ttatgagtaa aattatcggt attgacttag gtacgactaa
    15961 ctctgcagta gcagtattgg aaggaaacga agcaaaaatc atcgctaacc cagaaggaaa
    16021 ccgtacaaca ccttctgttg tatcattcaa aaatggagaa atccaagtag gggaagttgc
    16081 aaaacgtcaa gctgtgacaa acccaaatac gatttcatca atcaaacgcc atatcggtga
    16141 agcaggttac aaagtagatg tagaaggtaa atcatataca ccacaagaaa tctcagcaat
    16201 gatccttcaa tatatcaaag gattcgcaga agactattta ggtgaaaaag tcgaaaaagc
    16261 agttatcact gtgccagctt actttaatga tgcacaacgt caagcaacaa aagatgctgg
    16321 taagatcgct ggtctagaag tagaacgtat cgtcaatgaa ccaactgcag ctgctcttgc
    16381 ctatggttta gataaaacag atcgcgacga aaaaatctta gtattcgacc ttggtggcgg
    16441 tacttttgac gtatctatcc ttgaactagg agacggtgtc tttgatgtat tgtcaacagc
    16501 tggcgacaac catctaggtg gggacgactt tgataacaaa atcatcgact acatggtagc
    16561 agaattcaaa aaagaaaaca gcattgactt gtctcaagac aaaatggctt tacaacgctt
    16621 gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc gtatcaagca cacaaatcag
    16681 cttgccattt atcacagcag gagaatctgg accattgcat ttggaaatga cattgacacg
    16741 tgcaaaattt gatgaattaa cagctgactt agtagaacgt acaaaagtac cagtacgtca
    16801 agcgttaaaa gatgcaggtc tgtcacaatc agaaatcgac gaagttattt tagtcggtgg
    16861 atctactcgt atccctgcag ttgtagaagc agtacgtaaa gaaacaggaa aagaaccaaa
    16921 caaatcagtt aaccctgatg aagtagttgc tatgggtgct gctatccaag gtggcgtgat
    16981 cactggtgat gtgaaagatg ttgttttact tgacgttaca ccattgtcat taggtatcga
    17041 aacaatgggt ggagtgttca ctaaattgat tgaccgcaat acaacgattc caacaagtaa
    17101 atcacaagta ttctcaactg cggcagataa tcaaccagca gtagacatcc atgtattaca
    17161 aggtgaacgc ccaatggctg ctgacaacaa aactctagga agattccaat tgacagatat
    17221 tccagcagct cctcgtggta ttccacaaat cgaagtaaca ttcgatattg ataaaaacgg
    17281 gattgtaaat gtatctgcga aagatctagg tacacaaaaa gaacaaaaaa tcacgatcaa
    17341 atcttcttca ggtctaacag acgaagaaat cgaacgtatg gtgaaagatg cagaagcaaa
    17401 cgctgaagca gataaagctc gtaaagagga agttgaccta cgtaatgatg tagacgcatt
    17461 gctattctct gtggacaaaa cattgaaaga attagaaggc aaagtggacg aagaagaagt
    17521 gaaaaaagca gaaacagctc gtgacgaatt aaaagccgct gttgaagcaa acaacatcga
    17581 ggaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt caaaacttga ctgttaaact
    17641 atacgaacaa gctgcacaac aacaagctca agaaaatcca gaagcagctc aaggcggagc
    17701 agatgatgtc gttgatgctg attttgaaga agtcgatggc gacgataaat aatcagttca
    17761 ctgaatttcg agagaaggcc aaagttgcta ctttggcttt ttcttttgaa aaatagtttg
    17821 aagtatcggt ttttgtttca aacaaaatat gatatgataa caagcgacgt gaaaatgatg
    17881 agaaccaata ggaggaaagc ctatggcaac aaaacgtgat tattatgaag tcttagggct
    17941 ctcaaaagga gcatcagaag atgaaataaa gaaagcttat cgtaagcttt cgaaaaaata
    18001 ccatcctgac atcaataaag aacctgatgc ggaagaaaaa ttcaaagaag tatcagaagc
    18061 atatgagatt ttaagtgacc cgcaaaaacg cgctgcatac gaccaatacg ggcatgcagg
    18121 cacagatcca aattacggtg ctggtggagc tggtggcgga tttggtggat tcg
//
LOCUS       DAYLZO010000059        18124 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.060, whole genome shotgun sequence.
ACCESSION   DAYLZO010000059 DAYLZO010000000
VERSION     DAYLZO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.060"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(53..787)
                     /locus_tag="ACGVJG_001760"
     CDS             complement(53..787)
                     /locus_tag="ACGVJG_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFJ6557697.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACGVJG_001761"
     CDS             1114..1524
                     /locus_tag="ACGVJG_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFJ6557698.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACGVJG_001762"
     CDS             1517..2218
                     /locus_tag="ACGVJG_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFJ6557699.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACGVJG_001763"
     CDS             2218..3804
                     /locus_tag="ACGVJG_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557700.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACGVJG_001764"
     CDS             3823..4044
                     /locus_tag="ACGVJG_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFJ6557701.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACGVJG_001765"
     CDS             4041..4802
                     /locus_tag="ACGVJG_001765"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFJ6557702.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACGVJG_001766"
     CDS             5182..5691
                     /locus_tag="ACGVJG_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFJ6557703.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACGVJG_001767"
     CDS             complement(5766..6305)
                     /locus_tag="ACGVJG_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFJ6557704.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACGVJG_001768"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACGVJG_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFJ6557705.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACGVJG_001769"
     CDS             7318..8169
                     /locus_tag="ACGVJG_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFJ6557706.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACGVJG_001770"
     CDS             8197..8832
                     /locus_tag="ACGVJG_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFJ6557707.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACGVJG_001771"
     CDS             8852..9625
                     /locus_tag="ACGVJG_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFJ6557708.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACGVJG_001772"
     CDS             10033..10830
                     /locus_tag="ACGVJG_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFJ6557709.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACGVJG_001773"
     CDS             10808..11221
                     /locus_tag="ACGVJG_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFJ6557710.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACGVJG_001774"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACGVJG_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFJ6557711.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACGVJG_001775"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACGVJG_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFJ6557712.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACGVJG_001776"
     CDS             15998..16558
                     /locus_tag="ACGVJG_001776"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFJ6557713.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..>18124)
                     /locus_tag="ACGVJG_001777"
     CDS             complement(16643..>18124)
                     /locus_tag="ACGVJG_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFJ6557714.1"
                     /translation="LPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAA
                     DIPFWDFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLP
                     TVLAAHVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNA
                     IDMQLIADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVT
                     EPAMFGINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPF
                     IIAMIIAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSA
                     TGTAVATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTS
                     VFETKHAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEI
                     EQAGKKTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
BASE COUNT         5859 a         3295 c         3898 g         5072 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaag
//
LOCUS       DAYLZO010000060        17678 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.061, whole genome shotgun sequence.
ACCESSION   DAYLZO010000060 DAYLZO010000000
VERSION     DAYLZO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.061"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            58..342
                     /locus_tag="ACGVJG_001778"
     CDS             58..342
                     /locus_tag="ACGVJG_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557715.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            415..894
                     /locus_tag="ACGVJG_001779"
     CDS             415..894
                     /locus_tag="ACGVJG_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFJ6557716.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            1117..1260
                     /locus_tag="ACGVJG_001780"
     CDS             1117..1260
                     /locus_tag="ACGVJG_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFJ6557717.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            1605..1733
                     /locus_tag="ACGVJG_001781"
     CDS             1605..1733
                     /locus_tag="ACGVJG_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557718.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            1838..3421
                     /gene="abc-f"
                     /locus_tag="ACGVJG_001782"
     CDS             1838..3421
                     /gene="abc-f"
                     /locus_tag="ACGVJG_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFJ6557719.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(3858..5042)
                     /locus_tag="ACGVJG_001783"
     CDS             complement(3858..5042)
                     /locus_tag="ACGVJG_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFJ6557720.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(5067..6074)
                     /locus_tag="ACGVJG_001784"
     CDS             complement(5067..6074)
                     /locus_tag="ACGVJG_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFJ6557721.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(6246..6605)
                     /gene="comGG"
                     /locus_tag="ACGVJG_001785"
     CDS             complement(6246..6605)
                     /gene="comGG"
                     /locus_tag="ACGVJG_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFJ6557722.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(6577..7002)
                     /gene="comGF"
                     /locus_tag="ACGVJG_001786"
     CDS             complement(6577..7002)
                     /gene="comGF"
                     /locus_tag="ACGVJG_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFJ6557723.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(6965..7312)
                     /locus_tag="ACGVJG_001787"
     CDS             complement(6965..7312)
                     /locus_tag="ACGVJG_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557724.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(7284..7754)
                     /gene="comGD"
                     /locus_tag="ACGVJG_001788"
     CDS             complement(7284..7754)
                     /gene="comGD"
                     /locus_tag="ACGVJG_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFJ6557725.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(7729..8007)
                     /gene="comGC"
                     /locus_tag="ACGVJG_001789"
     CDS             complement(7729..8007)
                     /gene="comGC"
                     /locus_tag="ACGVJG_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFJ6557726.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(8004..9053)
                     /gene="comGB"
                     /locus_tag="ACGVJG_001790"
     CDS             complement(8004..9053)
                     /gene="comGB"
                     /locus_tag="ACGVJG_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFJ6557727.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(9010..9939)
                     /gene="comGA"
                     /locus_tag="ACGVJG_001791"
     CDS             complement(9010..9939)
                     /gene="comGA"
                     /locus_tag="ACGVJG_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFJ6557728.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            10071..11825
                     /gene="ade"
                     /locus_tag="ACGVJG_001792"
     CDS             10071..11825
                     /gene="ade"
                     /locus_tag="ACGVJG_001792"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFJ6557729.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(11923..12585)
                     /gene="trmB"
                     /locus_tag="ACGVJG_001793"
     CDS             complement(11923..12585)
                     /gene="trmB"
                     /locus_tag="ACGVJG_001793"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFJ6557730.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12655..13437)
                     /locus_tag="ACGVJG_001794"
     CDS             complement(12655..13437)
                     /locus_tag="ACGVJG_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFJ6557731.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(13641..14852)
                     /locus_tag="ACGVJG_001795"
     CDS             complement(13641..14852)
                     /locus_tag="ACGVJG_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6557732.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(14845..15582)
                     /locus_tag="ACGVJG_001796"
     CDS             complement(14845..15582)
                     /locus_tag="ACGVJG_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557733.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            15720..16154
                     /locus_tag="ACGVJG_001797"
     CDS             15720..16154
                     /locus_tag="ACGVJG_001797"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFJ6557734.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            16147..16491
                     /locus_tag="ACGVJG_001798"
     CDS             16147..16491
                     /locus_tag="ACGVJG_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFJ6557735.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            16671..>17678
                     /locus_tag="ACGVJG_001799"
     CDS             16671..>17678
                     /locus_tag="ACGVJG_001799"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFJ6557736.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         5637 a         3587 c         3122 g         5332 t
ORIGIN      
        1 aatttgctct tggcaaattg ctttttctat ttgttgctta ttttggagga tttgttcatg
       61 attaggattt taatgattat cgccacttta gtattattat tcgttagcta ttatttattt
      121 aataaacagg atatcttttt tgtattgatc aacaaaaatg ataaaaatca aggattccta
      181 caattttacg gagctgcata tgctgtttta ggcgtaatgg gtattttaac agcttttttc
      241 aatcaacgct ttattgcctt ggtcttttta ctgatcgtaa ttattgtttc tgccaccttc
      301 agtatccgat ttgcaaaaaa aattgctgaa ccaaagcagt aaataattct ctttttccct
      361 aaagtgttct acaataaaag agtacaatac gattgacttg aggagtgaat gcttatgtta
      421 caacaataca aaaaaatcat ggttgccgta gatggttctg atgaagctga attagctttt
      481 aaaaaggcgg tcaatgtagc tatccgaaat aacggagaac ttctattagc acatgtcatt
      541 gacacacgtt ctttccaaac cgtttcatcc tttgatggaa tgcttgcaga acaagctact
      601 gaaatggcaa agcaaacatt ggcagattat gaaagcaatg cgaagaaagc tggcttgaat
      661 aatgtaacct ctgtcgtaga atatggctca ccaaaacaaa ttattgccag agaaatccca
      721 gaagataatc aagtagattt gattatgtta ggagccacag gattgaatgc tgtagaacgt
      781 ttatttattg gttctgtttc tgaatatgtt attagaaacg cagcttgcga tgtgcttgtt
      841 gtccgaactg atttagaaaa ccaattgcca gctaaagacc acaccaaagc ttaacacgta
      901 gacgacctgc caactatcgg caggtttttt tgcacaaaaa accgtgataa aaacaaaaga
      961 cgcggcaagc actcatcggt ctttataatg tttaaaataa agggaaaaca gtcaatgagt
     1021 tcaagtaaaa taaaaaaaag tgaacaccag ggcgattagt cgccctggtg tggtttaaaa
     1081 cgacctaagc atgccaaagc ttattggttc gtcaatgtgg aagtttccga tgaatctgat
     1141 acgaaaaaat atgaagtatc gatggcggat accgatttta tcgccaaact aaaattttat
     1201 ttcgggttcg ggtaccgcga gaaggcaagt gctacattcg gtaaatcatt caagaattga
     1261 tgaaaggcta ccgcttgcca ttgcaaccac aacgatactc ctgaacgcga tgttggtgag
     1321 atttccaaag agaagagctg tttaatgcaa cggtattaag caaatggcaa aaggaagtca
     1381 tgtagacaaa gttttttatc aaacaccata tgatttcgcc tgaacgctgg tttagctcag
     1441 aatacaatga tgtggtccac gagacattac cctcagtcag ataaaaaaag ctctctgaca
     1501 gaattacaca tcgataaata ttaagtaaat gtcagcgcta gttgaacttt ctgacacttt
     1561 caggtacact ggaaaagaca actacacgaa ggagggaaaa tactatgatg cgcatgacta
     1621 aaataacaag taatgaaaat taccaacgat accatatcgc atttagccat gcaattggtt
     1681 ttacagttgc tgttgacggg acaagtttgt ccaaaacagt tgaattcacg taaaatcaat
     1741 ggccatctca aggtattttt ctcatgataa tctgagttta aataacacag gccatggata
     1801 accatggcct ttttgagtgg tctcaaggag agagatcatg atcatacaag caatttctat
     1861 taaaaaaaca atcggcggga atccattatt tgaggattta tcctgtcaaa ttgaagctgg
     1921 tgaaaaaatt gccttagtcg gtatcaacgg aactggcaaa tcaacctttt tacaaatctt
     1981 gaccggcaga gaaggtgtcg acagcggtgt tatcagtcgc aaaaaaggct taaagatcgg
     2041 gacggtcgaa caagaactta cagtttccga agcgaccgtc aaccactact tgctccattc
     2101 tgccgctgag atacaggacc tcaaacagca aatgagtcgc tacgaagcat tgatgacaga
     2161 accagataca aacttagaga agtgtctggc ccgttacggt gagctgcagc atcgctttga
     2221 agaactaggc ggttacgtat tagaagaccg gattgcagcg atcttgcaag gcttagggat
     2281 tccacaccgt caggaagcac ggttaacaga acttagcgga ggcgaacgag ttaaagtcgc
     2341 tttagccaaa attttagcag ccgatgccga tttactttta ctcgatgaac cgaccaatca
     2401 tttagatctt aacagtattc gctggttgga aaactattta cagaatacaa aaaaatcggt
     2461 tctggtcgtt tctcatgatc ggcaatttct cgatcaagtc accacaaaaa catgggaact
     2521 ggaagaaggt gcattgattg agtatcccgg aaactacagc cgttttcgag tcttgaaaga
     2581 agctcgcttg gctgagctga caaaaaatta cgaactgcag caaaaagaag tccagcggtt
     2641 gaaagtcatg attcgtcgtt ttcgtcagtg ggcacatgaa ggagacaatg agtccttctt
     2701 caaaaaagcc aaagaactgg aacgccgcct ggcgaaactg acactcgtca aaccgccacc
     2761 tcccccgaaa aatcggctgc aaagcttgtc taatggtgga aaatctggca aagaagtttt
     2821 catcattcaa aacttgcacc agcagtacgc tgatcaagta ctattcaaag acagcagttt
     2881 tgcagtctat cgtggagatc atttagccat catcggcgac aatggtgcag gaaaatcaac
     2941 cttactcaaa ttattactcg gtgaggaaaa gccttacggt ggcaccatca aattaggtag
     3001 tagcttgcaa atcggttatt tgccgcagca gctgcagttt gccgatcccg atagccgttt
     3061 gctggcttac gccatcacat tgactggaaa cgaagaagtc gctcgacggc aattagccaa
     3121 aagtggcttt tatcaaagcg atgtgggtaa acggatcaaa gatttatccg gtggtgaaaa
     3181 aatccgtttg gcgttgctga aactcttttt agaaaaaatc aacgtcctga ttttggatga
     3241 accaaccaat cacctcgaca gctatgctcg agaagaaatt gaagaaatgc tgcaagacta
     3301 tcaaggaacg ctcctagcag tcagccatga ccgctacttt ttgcagcaac acttccaaga
     3361 agcactcgtt attgatcagg agcagatcag ccgccagccg ctcggccaac tgattgacta
     3421 ggacgttttt tcgcaacaaa aaacttgatt ttcatacctg tgctttcacg attcaggcaa
     3481 agtacaggtg gaaaatcaag tttttattat tcgatcaatg aatgtttcgg atcggttttc
     3541 tagctaaaca taaagcgaag aataaagtcg acagcccacg aggctgatct aaaaatgttt
     3601 tttcttttta catatgtaat gtcccttata aagatgaatg aaaataaaat attgggattt
     3661 tcctgaaaat caataaataa aatgccagtc ttttcccttt tgaccacttt tccaagcaaa
     3721 attccctaag aagatcctgt aaaaaagaag ggaaattgaa gttaaattac cattttgcgg
     3781 tcttctccgc aaaaaaaaca catgcaagaa ttccctcttg catgcgtttc atcttttatc
     3841 ctaacgaatg ctttggctta gccgcgcaga cgctccacat cacgggcaat cattagttcc
     3901 tcatctgttg ggatcactaa tactcttact tttgattctt ttgtagagat atctacttct
     3961 tcaccacgca attcattcaa cgctggatcg atttcacaac caaaccaaga catacctttg
     4021 atgatttcac tacgtacatg tccgtcattc tcgccgatac cagcagtaaa gacaattgca
     4081 tcaacaccat tcatcgttgt cacataaccg ccgatgtatt ttcggatgcg gtccacgaaa
     4141 atgtcataag ctaaacgaat atgttctttt tcatagttct tttcaagatc acgcatatca
     4201 ctagacaatc ctgttaggcc tagcagacca gatttcttgt tcaaaacatt gatcatttca
     4261 tcaatatcaa tctctaattt ttccattaaa tatgggagaa cagctggatc gatatcacct
     4321 gaacgtgtac ccattgtcac cccagctaat ggtgtgaatc ccatagaagt atccatggat
     4381 ttacctccat cgactgctgt gatcgataca ccatttccta agtggcaagt gatgattttt
     4441 agttcttcga tcggtttgtt caataatttt gccgcacgtt ccgacacata acgatgactt
     4501 gtgccatgga agccatattt tcttactttg aaatcttcat aataagacac cggtaagcta
     4561 tataaataat tttgctttgg cattgttgag tggaaagatg tatcgaatac agccacactc
     4621 aaaatgtcag gtaaaatctt tttaaacgct ttgatcccca ttaagtttgc aggattatgc
     4681 aaaggagcaa attctgctaa ttcagcaatg cggtccatta ctttctcatc gatcactact
     4741 gaatccccat aagtttctcc tccgtgaaca acacggtgcc ctactccggt gatttcatca
     4801 taagagctta aaatttgcag ttcaatcaac tgatctaaca gcatttttac tgctacttcg
     4861 tggttttcga tatccatgat ttgttcgaat ttttgattat cgccgtactt gatggtaaaa
     4921 atagaatctt gtaaaccgat acgttcgacg atccctttcg caactacggt ttcttctggc
     4981 atttgatata attgccattt caaacttgaa ctcccagcat tgattgcaat tgttttagac
     5041 atatgactct ccttctcgtt gaatgattac agagcagatg atttccagtt tttaaactcg
     5101 ttgaaaaatt ccgtcacctt ctctggttct tttaatgaag caagtttcac aagcaagact
     5161 tcttttactt gttgtgcttg tggtcctttt ttctgtaaga tcaaaatact tttttgcgat
     5221 tgtttattac ggaacaattc atctggcaat tgaatcattc cttgaagata accttcttct
     5281 ttaaaccatt ttttgatttc ttcactttga tccgtttcta aaaatccact cggcatcaaa
     5341 aacaaaccaa aaccatccgg cttcacatat ttcattgatt gctctagcaa taaatgatgt
     5401 gcataactat ggccttcttc ggtacttgtt aaaaactctt ttgctttttc gtcattcgga
     5461 taataaccga ttggcaaatc gctgattgca aaatcgacag gatctattaa taattcctgc
     5521 aacccgtctt gatgaaagta ttgaacattt gcttgtgtca aatcacttgt agaagctgcg
     5581 acagccaaca atgtatcatc aatatccaca ccaattcctt ctgtttggta ccccgcattc
     5641 gataggttta aaagaactgt taataacaag ttgcccattc ctgccgcaat atccagaatc
     5701 ttaaccggtg cttttttatt tgtgtatagt tgttcaataa gaaaaacaaa taaaaaaccg
     5761 atactatcag gagtgagttg atggttcgcc tgcaaatgtt ctgtctggct gcccttcaat
     5821 aaaagtaatt gagacagtct gcgccattct tctggctcga aagaaagttt cttcaactct
     5881 tcataaagag ctgtgatccg ttgcgttgtc tcttttgtcg gcactccatc caccacacgt
     5941 acttgataat catctattaa attttctgca ttttcgacat aggcttctaa aaaggaagtt
     6001 cccaatgcat tctgtaaaag ctgaatagct tccaggtttt gattaaaccc ttgttccatt
     6061 ttttctggta acatggtgca cctcatttct agatatatta taacaatttt ttcacctgtt
     6121 taattctatc gaacaattga caaaatttca atcgttttca ctgtttttta tttgtgaaac
     6181 ggcaaacatt tttttattaa aaacagtgtt ataacagaaa agagggcgtt ttcacgaaca
     6241 ttccatcatt ctgtactagt atctgtatca ttttttactt cttctgtgaa ccgaaagata
     6301 aacggtgaga cttccacctc taagaatagg gactgttgta cccactgata tgttacagat
     6361 ccagttgtgt agtaaaaaac tccttccgaa ctttcggcaa gcgtagactg ttccgataaa
     6421 gccatctgct tcattatttt tgctatataa taatttttga ttcgtatatt aagatccatt
     6481 gacaaacggt agctttctaa tgctgccatc aataaaaacg tacataaaag aaataaaagc
     6541 aagacagaaa ataaaataga gcccttataa cattttctac tgatcgtttt ttgtccatat
     6601 ggcatatcca tcttctccat tttcaaagtg tacataaaaa gaaattgtgt gttctttttc
     6661 caataattga agctcagtga tacttgttaa cataggttga tgtccgccgg aagttcttct
     6721 tttacgtatc attgcctgat atctttcgat gatatactgt ttatcctcga tcgtgtagtg
     6781 cagttgctga tttttcacag agacattgtg actgctcttg agttcttctt ctaattgtgc
     6841 taaaaatatt agccactctt tttgatcttt acgctgcaaa aattcactta cttggatggt
     6901 ttgctgaatc aaaagattga atgtgagcaa tagactgctc aatatcatca gtccaattag
     6961 acattcaatc agcgtaaatg ctggaattac ttgttttttc aatactgatc gcttctttct
     7021 ccatattgac aaacactttt tcgattactc cattattatc gacaatttgg atatcgtatg
     7081 ggatcatttc ttcccttcgc tctggtcttt tgtgatacat ccgctgcgtc ttgatatctt
     7141 cataaatcat tcgaataaat agcagttcct ccccactttt cttttctgcc tgaagcaaaa
     7201 acagctggcc aaagctcact aagtagacac agacagaagc gatcataagc gctgtaaggg
     7261 actccaacaa aacaaatcct gttttattca attctttttg tatagcgacc actccctatc
     7321 tggaattgat acgtaatcgt ccgctctttt tcttcccaat aaaattgata agcttttagt
     7381 gaactttcat tcccggttct agcagcaaaa gtaatagaag catggcgttt caaggtaatc
     7441 tcatcaggaa tatctaatac ttcccaattt attttatctg gattcggaac atgaaaaatg
     7501 atttgattct cttcactatt ccaataaaag cctgtttgga tctgattcac aatagcgatt
     7561 ttttgcgtag aatagattcg tttttcaaat tggccgaaaa attgatagac tgccaattca
     7621 ttcttccatg cagaaaatgc gagaactgga aaagaaagga tcgttgtaag taataaaatc
     7681 agtaagagtg attcaaacaa cgtatatcct gatgctaaaa ccttaccttc aaggccgctg
     7741 atagatttct gcatgttctc gtttgatata gccttcattc aacaattcct ccacagtagg
     7801 aactcttcca ttattttgaa gcgcatagag ctctttttgt gtgtctacca actggatgat
     7861 cgcttcacga ctttcttggt ctacatgatt cctataacgc gacaaattag ggacgaacaa
     7921 caacactaat acactgatga tcagtaagac gattagcatt tcgatcaatg taaaaccgct
     7981 ataataagat atttttctct ttttcatggc aaaacctcct ctatgcttcc gtaaacaggt
     8041 aataaaacag cggcataaac taataaaatc aaagccgcta cgcccaaaaa aatgattggc
     8101 tgaataaaat gcatccagtg atgaagacgt ttgaaaagct gttttcttgt ccattcgctg
     8161 taaaaaatca gttctttacc taaacttccc tttgcctctc cctgtaaaac aattttactg
     8221 aattcttctg atagaaaacg ataattctgg aactgctcag ccaatgacat acctgcctcc
     8281 aatccttgaa tcaacttaga ggcaagatgc tgaatcagac tttcttgttt cgtttccttt
     8341 aagcagacta tgatttgcct taattcgaaa ccttcatgaa aaagctttcc aatctctagt
     8401 gacaaataag ccgattgata caaggaatat atcggtccaa tcaataattg tctgcttatc
     8461 cattcactct gatcaacagg agacaacttc atcagacgat accttactag taaagccaat
     8521 agagtaaccg ctaggatgaa tagtagaaca taccaatgaa agacttgtat cagctgaata
     8581 ccccaatgct cttgcatgac cattcctgaa ccaataagct gaggcaaaac gaacaaccgc
     8641 atactaaaca agatacctag caaaaaggtg agcaatagca tcggatatgc aagcagcttt
     8701 ttcaaatcat tgcgaaatcc ttcaacaagc cgaaactgtt tggcaatttc caacaaagtt
     8761 gtcggcagat tcccatgacg ttcagctaac tcgatctgta ccagttgctc tgctccatat
     8821 cctatttgcg aaagtacctc actaaacgct tgcccttcct ttagctgatg ttggatattt
     8881 gccaaaataa caggtggaaa cgaacggatc gtgagtaata aataaaggga ttcttgcaga
     8941 ctaaaaccag ataaaagcag ttctcccatg gtttgtgcaa actggttttg ctggcttctt
     9001 gttaatcctt tagaatagtt ctgactgcca aatattttcc tgagcttctt cataactttc
     9061 ctcccatgat ttttttactg attcttcctc cataaattga taactccata aaacagaagg
     9121 atggtgattg gcatcttcca aaagttcttg ataaatgaca ccagctaaac attcagatag
     9181 ctcttctttt ttctttgtca gttcatggat tctgaccaat gtaccttcaa gattgcgtgc
     9241 atgtacggtt gcaaaaaccc ggtgccctgt tagtgctgct cttaccgcag catgtgcagt
     9301 tgattcatct cttatctcgc caatgattag caagtctgga cgatgccgta atgcgagtct
     9361 caaaagtgta tcataagtct gtgctatttt cggattgatc tgtagctgca gaaaaaacgg
     9421 ttcttctatc tctacaggat cttcgatagt aatcacttgc aatttttctt ccaaagccaa
     9481 ccgatacatt agagaagtct tgcctgatcc gactggtccg ctgaaaaggt ataatccacg
     9541 tctgacggtt ttctgtaaaa tagttgcaaa atctcgagaa ctaaagcaac gatggggtgt
     9601 ttcaaaagta ccgtataata gccgaatcac taaactctct cgttgaaggt aatctcctac
     9661 agaagacaac cgcagacgaa cttcctgttt ttctaaagaa taagtaaatg cacctaactg
     9721 agcttttctt ttttctccga tatccatctg tcccaaatac ttaaatcttg agatcagttg
     9781 ctgtccatct gccgaagaga ctttcttcca gaatgttcgt ttttttccat agcgaaaata
     9841 gatgtaaaat acttctgaag ctggcaatat atatacgtct tgggcatgtt tggcaaaact
     9901 ttcacttatc atttgatccg ataatcgttt tatttccatt attctcccct ccacaaaaaa
     9961 catacgtctt tttgatcttt ttgtttcaaa taaaatgaat ttacaaaaaa atattggcaa
    10021 aaaccgaatt tatgttatac ttataaaaat ttatcttggg aggcactcca ttggaaacaa
    10081 cattaaggaa atatatcgaa gtagcaagta aaaaaaagcc tgctgaccta gtgatcaagc
    10141 atgctaagat cgtcaacgtc tttacgaaag aaatcatgga aaaagatgta gcaatctgcg
    10201 aaggaatgat cgtaggaatc ggtgaatacg aaggaaaaag cgtacataat gctaatgggc
    10261 aatatctagt gccaggattc atcgatggcc atgtgcatat cgaaagctca cttttatcac
    10321 caagcgaatt ttcaaaagta tccttgcttc atggagttac tactgtagtg acagatcccc
    10381 atgaaatagg aaacattgct gggagtacag gtcttgagtt tatgatagaa gatgcaagac
    10441 aaacactcat gaatatcttt tttatgctac cttcttgtgt acctgtaacc ccttttgaga
    10501 ccaatggtgc aaatttaaat gcaaaaaaat tagccccttt tttacaaaaa cccgaagttt
    10561 taggcttagc tgaagtcatg aactatcaag cagtagcaaa taatgaaaca gacatcctag
    10621 aaaagatcca attgatgcac cagcataaga aaaaaattga cgggcatgca gctggtatcg
    10681 gcatggaaga gttaaatgtt tatcctgcag caggtattcg gacggatcat gaagctacaa
    10741 ctgcaaaaga agctaaagaa cgtttggatc ttggtatgta tttaatggtt cgagaaggga
    10801 ccgttgcaaa agatctggca tcgctactac cggcgatcac acctgaaaat tcaagaagat
    10861 gcttctttgt caccgatgat aaactgatca atgatttagt aaaagaagga tcaatcgatc
    10921 atatcgtccg tcaagctatt gcacttggaa tcgatccatt acaagccatt cagatggcaa
    10981 cattgaatgc agcagaatgt tttgatttga aaacactagg tgcgattgcc cctgggtatc
    11041 aagctgattt cttcttaaca gacgatctta ccaagctgcc aattcatgaa gttttctata
    11101 agggaacatt agttgttcaa tctgggcaac tccaagacca tttatttgct gatcattcaa
    11161 attcatatac gtgttgtctg ccaaaactaa acgctcaacc attaaatcca cattcgttag
    11221 agctgcttct ttcttcttca caagcccatg tgatcgaaat tatcccgaat agtctagtga
    11281 ctaaagattt ggtagaaaaa gtggaccgac aaggcgaacg attcattcct tcaatcgaaa
    11341 aagaccaatt aaagattgcg gtgatcgagc gtcaccatca aacgggaaac attggattag
    11401 gcatagtgaa aggatttcag ctgaaacaag gagcgatagc gacaacgatt gcccatgact
    11461 cgcataatct agttgtcgtt gggacaaacg acgaggatat gctgtatgct gctaatcttt
    11521 tgattcaaaa aggtggcggg atgaccgttg tcaacaatca gcaggaaata gcttgtgttt
    11581 ctcttcctat aggcggaatc atgacacagg aacctttttc gatcgtctat gaacagttga
    11641 atgaattaat aactaaagca catcaactag gagcagctaa aacctttgat ccttttttga
    11701 cactttcttt cttaacatta tctgtcatac ccgaattgaa gatcaccgac aaaggattat
    11761 tttcattttc tcgcttccaa ttgattccac cttgtggaaa aaccgatgaa ataaatacat
    11821 tttaaatcaa ataaatccca gaaaatacat tacatctgta atgttcattt tctgggattt
    11881 atttatggat attaatgttt ttatgaaagc agaaagttag tttcacagac ttttcttttt
    11941 ggtcttatct tgaaattgag cttctactcg ataaatacgt tgaccttttg aagaaaattt
    12001 ttcttcatac tccgtcataa tattgccttc aaattgactt tgatgtaaat ctaaccatac
    12061 ctgtttgagg atcatgccat attgagaaaa acttgccaga gaatactcaa acaatccttg
    12121 attatctgtt ttgaaatgga tttctccatt tggacgaaga atctgttcat ctacagcaag
    12181 aaaagtctta tatgtcaatc gccgtttttc atgacgtttt tttggccatg gatcggaaaa
    12241 attcagatag atttgatcta cttcgctgtc agcgaaatac tctgtcaacg ctgatccatc
    12301 cacatgtaga agtttcaggt taggaagcgg ttgttcgatc aacttgtcta gtgcaatcga
    12361 aacaacactg acctgcattt ctactccaat ataattgatt tcaggatggg cttttgccat
    12421 tccagtgatg aactggcctt tccccatacc aatctcaatg tggattggat gatcgttttc
    12481 aaacaattca ttccattttc ccttccacaa tgttggatca ctaattacaa aattcgggtg
    12541 agccgctaac atttccgctg ctcccggacg atttcggaca cgcattattt atctccattc
    12601 ctttcctcta attaaaaagg tctggccaaa tctccgcaga gtttgggcca aacctcagcc
    12661 actgaaaatt cgttttaatt gtaaaatttc ttcgttcatc cgtcgatgat cttcacgttt
    12721 gcaatatctg gtgatttctt ggagcaaatt catggccgca taccaatgaa atttttcaag
    12781 catttcgtca gtcgggcgta tgccataact gatcagccac tgattccagc tagacagtgg
    12841 cacataattt cctaaaatcg ttgcaatatc caccgcaggg tctgcaaaca tgattgaatc
    12901 ccaatcgact agatataaat aatttttaca tactaaccaa ttacgatggt ttacatctcc
    12961 gtgtactact gataagtttt ccttatctat tgacgggatc tgctcacgaa gaaaacgatg
    13021 gacaatttgc agaaattgat ttttctggag ttcttccggt aattcttttt catatttttg
    13081 cagcatcttc tccggcgtca tgacacgacc accgatacgg gtcagcatgg ttttaagaga
    13141 atttgaatga tgtaaatggt acaaaacatc aatcacgtcg tttctttgac caatctcatt
    13201 aggagtcagc aagcgacctt ccagccactc ttgtgctgtc aaagtatcgc cattccccgt
    13261 tcgttttgtc caaacaagtt ttggtgtaat tccttccctt gaaagagcag ccaacatcgg
    13321 agtcgtgttt cgtttaataa agactttgtc ctcatcacgc atgcctatgt atgtttttcc
    13381 tgtgtcgcct ttgatgggac gtaaccgcca ctcttggtct aactgaaact ccatcatgca
    13441 ttctcccgtc ctcttactct ttgttttgca gtacttatta ttctagtcgc ctgaacacta
    13501 cacgtcaagc acatatacgt tctttttcat ttaattgcca ttcaaaaaac cagcttttct
    13561 caaaaaaaat agaacgaaca gcattcttac tgtcaacgct ctatttttca ttctcatttt
    13621 taccctacac agctttgttt tcagtctgac atcttcttca aacgagttgg tacatatagt
    13681 ttaacaaaga gcatagtaac aatcaaataa gccgctagcg gaacccatcg ctgctgccca
    13741 tctaatacga ctgcagcaat caaaccaaaa ataaccgacg cgataattaa tacccatgtg
    13801 agtagatact gaatcgcctg cttcttctga ttcttatgga ttggataaag ctgaacgaga
    13861 accatatagc ggaactggtt cgctaaaggt aacagttgga aaccgatcaa atataaaaat
    13921 aagaaaccga taatcagact aaaataaaag tcgactactc cggcaatcaa taacccacct
    13981 ataattacta atcggaaata aagaccacta aaatcagaac ctcttgccaa tcttcttgca
    14041 aacaagtaaa gataagtgtt ttttgattct cttttaatat gagtcaatat aggatccaaa
    14101 tagcgtcttc ttttgatctt tgccgtaatt tcaggaacat ctgtgaataa attgataaat
    14161 tgatagatct gatgcaacct atgttcttct acatgcacca ttttctccca atccaatcct
    14221 ttttcttgtt gcttccacaa aaaccagtaa tatagacttg cctgtactaa tgcacctgct
    14281 attcctgccc atattagcca ataaaatgat agaaacaaaa agatcagaga agaaaaaaac
    14341 cagcctaatt tccaataacg aataacttgt tgatctagtc gaaacaattc tgctctcttg
    14401 atttgcagat gactgaattt caaactccat aatatgatca gatagacgaa aaacatcgaa
    14461 aaggattgct ccgtactaac caccactaat ggcatggcca atccgcatgc caatatcaaa
    14521 aagatcaacg gaaaaatgca agaatagcga aaagctcgtt ccaaataggt ttgcatttct
    14581 ttttctttag gaagtaaaaa aacaatatct gctgcctctg caaaagtagc catcttaccc
    14641 acgaataaac agagcatcca aaatacagag attatgagcc ctccaattgc aaacggtggc
    14701 tgcagggttt tcaaccaatt agaataatat aatcccacac cgccaatcaa gaaaagacaa
    14761 actaaaacaa aatgatcgtt caatacataa cgcatatatt tcatcatttt cttttgatgt
    14821 cttgctaaac gtttttgaaa aaaactactc atgtgcctgc tcctttgtta aagcaatata
    14881 gatttcgtcc aaagaagaat ccggcagctg aaattctttt ctcaacatct caagagaacc
    14941 gtctgctcgt atctctccgt tatgtaagac cacaaaacga tcacaatatt tttctgcagt
    15001 agctaaaata tgcgtagaca tcaaaatacc ggctccttct tttttcattt ctaccatcaa
    15061 ttcaagcaat gcattgatag ctagtggatc caagcctaaa aatggttcgt cgatgatata
    15121 taaagaaggc tcgatcaaaa aggcgcataa gatcatcact ttctgtttca ttccctttga
    15181 aaaattagct gggaaccagt caagacgatt agataaccga aaagtctcca acaacttttc
    15241 tgctcttttc attgctatat ccataggtat atcgtaggcc atcgcagtga cttcgatatg
    15301 ttctctcaat gtcaattctt catataaaga aggagtctct ggaatatacc caatttgttt
    15361 gcgatattct tccgtatttt tctttaagga tagatgattg atttctatcg ttccttttga
    15421 agcttccagc agaccgataa tgtgcttgat ggtcgtactt ttccctgctc cgttcagtcc
    15481 aatcaaccct actatctctc caggctgtac atcgaaactg atatctttta acacagggag
    15541 atgtccgtac ccccctgtga catgatcgat aattaaactc attctttttc ctccgccatt
    15601 tatttttatt tattatacca cgaagaaaat gacggtattt tgtttttttc cttacaatag
    15661 ctttaactta tggtaaagtt aagctgaagt atttattctt tatagaaagg atgttttata
    15721 tggaaaactg tattttttgc aaaatcatca atcaagaaat cccaagctat aaaatatatg
    15781 aagacgacaa ggtctatgct tttttagaca tctctcaagc aacgaaagga cacacattag
    15841 ttgtgcctaa acgtcatgta gcagatattt ttgaatatga tcccgaactt gcaggagagg
    15901 ttttttcgcg tgtgcctaag attgctcaag cattggaaaa agcttttcca gaaatgaaag
    15961 gtcttaatat cttgaataac aaccgtgaac tagcctatca atctgttttt cattcacata
    16021 tccatttagt ccctcgctac agcaaagagg atgatttttc tattcacttt gtcaatcatc
    16081 aagatagtta tggatcggaa gaactaaaag ctattcaaga aacaatcgta aagcaggtgt
    16141 cctgcgatga ttaaaaactt tttcaaagga ctgttatttg gagctgctgc tggcactgtc
    16201 ggaggtctgt tattggcgcc caggagcggg aatgaaacaa gaaagaaact gatcgaagaa
    16261 ttggaagaag caacaactct gacaaatgat ttaaataaca gcctgaatca ttttaaaagc
    16321 gcattgatcg aaacaaaaga aacagctgaa tctgtgattc ctgctttcca agaagcaatc
    16381 aaaaaagata tcgaagattt taaattccaa gccgaaccac gtatcacaca aatcaatgaa
    16441 caagtagaaa aactaaaagc tgatctgcca gagtatcctg aacaaatata ataatgatta
    16501 ttttctcaac agatttaact atataagtga gaaaagtttg cgtatttcct aggaatgtga
    16561 tgaaattttg aattttgttt gaaactacca cgacaacgtt tttaaaggaa atgggatatg
    16621 gtatagttaa tatataaaaa taataactaa ttacaggagt gcaaatttta atgaaaaaaa
    16681 aatcaattat tttggcagct actagtgcgt tggctgtttt aactctagca gcttgttcag
    16741 gcgatactaa taaagatatc gcaacgatga aaggcggaac gatcactgta tctgatttct
    16801 atgatgaagc aaaacttgaa tcatcgaacc aatccttagt acaacggatg atcatttata
    16861 aagtattcaa taacaaatat ggcgataaag taaccgataa gcaagtggat gcagaatacg
    16921 acaaacaagc gaaatcatta ggtgatactt ttgaatcaca attagaagct gctggatata
    16981 cgaaagatac ttataaagaa tatattcgta acaatttagc ttttgaagct ggcttgaaag
    17041 ctcacgtaga tattacagat gacgatttga aaactgcttg gaaatctttc catccagaag
    17101 tagaagcaca aatcatcaaa ttatcaagtg aagatgaagc aaaagatgtg aaaaaatcag
    17161 cggatgatgg agatgatttc tctaaattag ccaaagacaa atcaactgat acagagacaa
    17221 aagaagatgg cggtaaagtt aaattcgact caacgacaac aactattcct gcagaagtaa
    17281 aagaagcagc tttcaaattg aaagacggtg agatctccga tgttattact acaacaaatc
    17341 ctacttctta tgcgacagaa tactacgttg tcaaaatggt gaaaaaccaa aataaaggca
    17401 atgacatgga taaatacaaa gatcaactaa aagatatcgc tactgaaact aaactaagtg
    17461 acaatgcatt cacaactaaa gtaatcggtg aagaattaaa agatgcaaat gtaaaaatca
    17521 aagatgatgc ctttgaaaat gtgttgagtg cattcacaac aacttcatca agtacgaaag
    17581 actcaagtga aactacagct tcaacaaaga gttctgacac aaaatcaact gattcaacaa
    17641 aagaatcttc aactgaagaa acaactgatt catcaaaa
//
LOCUS       DAYLZO010000061        17429 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.062, whole genome shotgun sequence.
ACCESSION   DAYLZO010000061 DAYLZO010000000
VERSION     DAYLZO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.062"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(405..2480)
                     /gene="glyS"
                     /locus_tag="ACGVJG_001800"
     CDS             complement(405..2480)
                     /gene="glyS"
                     /locus_tag="ACGVJG_001800"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFJ6557737.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="ACGVJG_001801"
     CDS             complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="ACGVJG_001801"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFJ6557738.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3783..4595)
                     /gene="recO"
                     /locus_tag="ACGVJG_001802"
     CDS             complement(3783..4595)
                     /gene="recO"
                     /locus_tag="ACGVJG_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFJ6557739.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4738..5637)
                     /gene="era"
                     /locus_tag="ACGVJG_001803"
     CDS             complement(4738..5637)
                     /gene="era"
                     /locus_tag="ACGVJG_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFJ6557740.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5652..6047)
                     /locus_tag="ACGVJG_001804"
     CDS             complement(5652..6047)
                     /locus_tag="ACGVJG_001804"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFJ6557741.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="ACGVJG_001805"
     CDS             complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="ACGVJG_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFJ6557742.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6524..8716)
                     /locus_tag="ACGVJG_001806"
     CDS             complement(6524..8716)
                     /locus_tag="ACGVJG_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFJ6557743.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8740..9711)
                     /locus_tag="ACGVJG_001807"
     CDS             complement(8740..9711)
                     /locus_tag="ACGVJG_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFJ6557744.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10417..11796
                     /gene="brnQ"
                     /locus_tag="ACGVJG_001808"
     CDS             10417..11796
                     /gene="brnQ"
                     /locus_tag="ACGVJG_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFJ6557745.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11952..12398)
                     /locus_tag="ACGVJG_001809"
     CDS             complement(11952..12398)
                     /locus_tag="ACGVJG_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFJ6557746.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12425..12601)
                     /gene="rpsU"
                     /locus_tag="ACGVJG_001810"
     CDS             complement(12425..12601)
                     /gene="rpsU"
                     /locus_tag="ACGVJG_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFJ6557747.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12763..13191)
                     /locus_tag="ACGVJG_001811"
     CDS             complement(12763..13191)
                     /locus_tag="ACGVJG_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFJ6557748.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13283..14143
                     /locus_tag="ACGVJG_001812"
     CDS             13283..14143
                     /locus_tag="ACGVJG_001812"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFJ6557749.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14227..14577)
                     /locus_tag="ACGVJG_001813"
     CDS             complement(14227..14577)
                     /locus_tag="ACGVJG_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFJ6557750.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14593..16320)
                     /locus_tag="ACGVJG_001814"
     CDS             complement(14593..16320)
                     /locus_tag="ACGVJG_001814"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFJ6557751.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16533..>17429)
                     /gene="trxB"
                     /locus_tag="ACGVJG_001815"
     CDS             complement(16533..>17429)
                     /gene="trxB"
                     /locus_tag="ACGVJG_001815"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFJ6557752.1"
                     /translation="PAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAEIENYPGFDSI
                     MGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKTVIIATGCIHR
                     KLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLTQFASEVVIVH
                     RRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVKTGNEFDLAAD
                     GVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVREKDLRQITTAV
                     GDGGIAGQQVYKYLESLEETTA"
BASE COUNT         4741 a         3707 c         3152 g         5829 t
ORIGIN      
        1 ttttacactc tctgattgac gtcataaaaa gtagctttga attcataatc gtttatataa
       61 cgattaatag aaaacaatat agtatttagt gttcatacaa tgattctaag atcattttga
      121 attaatcagt cttatagttg atttctaaat tgtcatatat tattaaaaat ttttgccatt
      181 taaaaagaaa agcgacagtt taccaataaa tttcaactga attatctaaa agtagatcag
      241 ttagtttaga taattcagtt gaaattgacc gactcaaatt acttgtataa tacctaggaa
      301 aaaattaact agcaccacgg gtatagttat ttatatttat aacaaataaa ccagagaaat
      361 agcctatttt gtgcctattc tctggtttat ttacatatta gaaattattt tgtgatgatt
      421 tgatctaaac ttgctagcga caaagccatg gcagcaattt gacttaattg cgttaatcgg
      481 ttcttacgta ctgctggttc ttctgccatg accatcgtct gatcaaaata attttcgatc
      541 agtgggcgaa ggcttacaag ctgatcataa ttttcgttga tcgtttggtc agcaaagctt
      601 tttttcattg cctctactgc tttgtgcagt tctttctccg cttcattttc gaacagttca
      661 gggtctactt ctgattgttt ttcaagctcc gcttttttcg ctaagttcac gacacgtgtc
      721 aaagcttcca tcgatggttt gaagtcttca tctgcaagat gctgcttgaa caattgtgct
      781 gctgcaaata cttttgtcag atctttttgt tctgcattca aaacagcttc gatgacatca
      841 tgacgaatat tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcacc
      901 tgttgttgcc cttctgataa accaatgccg aaacgatccg tatctttact gataacatca
      961 ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgatccga
     1021 acgacaccgt atgcttggcg acgtaaggca taaggatcgt ttgacccgct tggaatcatc
     1081 ccaactgcaa agaaagaaaa aatgctatct aatttatctg caatcgctaa aacagaacct
     1141 actgtagact gaggtagctc tccttcactg gatataggca tgtaatgctc acgaatcgct
     1201 gttgctacag caggtttctc gcctttcagc agagcatatt tttcgcccat gatcccttgt
     1261 agttcaggaa attcccctac catgttggtt actagatcaa atttatagat ttcagcagct
     1321 cgttgcagat ccatcaattc ttcctcagat agtcctactt gttcgccaat gatacgggca
     1381 atgacttgta cccgctgcat tttctcatag atactaccga ttttttcatg gaaagtaaca
     1441 gtttttagtt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat
     1501 tccgcatctt ctaaacgagc cgtcaatact ttttgattgc ctcgaaccac attttccaat
     1561 tttacccgat caccattacg agcagaaata aaatgaggaa gcagcatacc ttgatcgttc
     1621 cgtacctcga aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca
     1681 gaaagatatt tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg
     1741 acttcttcta gtaaatcttc gtctagatca ataacccagt gatttttttc ggctaattca
     1801 tttgcttgtg taacgatcat ttcttgtctt ttttgcggtg aagcaataac gaattgttca
     1861 ttcaattttt cttcatattc ttttgcatgc gcaaatgtca catcttcgcc taagaaacga
     1921 tgtcctcgag aggctctgcc tgttttgaca tctaagatct caaacgggat tacttcgtca
     1981 tctaacaatg ccacgatcca gtgaataggg cggatatatt caaaatcgta ttctgcccaa
     2041 tgcatcgtta ctgggaaagt gaggctttta atgacatctg ctaaacctgt caatacttct
     2101 ttcgcttctt tgccgtgagt gtatttcgtt acatagacat attccacgcc ttttaattct
     2161 ctaaaagtga tagcatctgt cgtcagacct tgtccgcgga caaatccttc agctgctttc
     2221 gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttca
     2281 ctgtctgctt gacgttctgg aatgtttgtt actttgattg ctaagcggcg cggtgtagaa
     2341 aacgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct
     2401 tctaattgtt tcatgcttgg tgtgacaaca tgagcaggca tttcttccag cccgatttca
     2461 agtaataagt cttttgccat cttattcagc ctccttcatg ttttctggat ttgctgtgtt
     2521 tttattcaat aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc
     2581 acgagccatg ttgcggatac gggaaagata acctgctcgt tctgttacgg atactgcacc
     2641 gcgagcatct aataaattga atgtatggct gcatttcaaa atataatcat acgctggatg
     2701 aacaagattt tcatcgatac agcgtttagc ttctttttca aaacggtcaa agttgcttag
     2761 tagcaagtct tggtcactta cttcaaaaga atatttggaa tgttcatatt ctggttgttt
     2821 gaagatctcg ccatatttca caccatcagc ccattccaga tcatagacac tttcaacttc
     2881 ttgaatatat gaagcaagac gttctagtcc ataagtgatc tcagaagtaa ctggtttaca
     2941 aggtagtcct ccgacttgct ggaagtacgt gaattgtgtg atctccatcc catcaagcca
     3001 tacttcccaa cccacaccgg cacaccccat cgatgggttc tcccagttgt cttcaacaaa
     3061 acgaatatcg tgttctaatg gatcaattcc taaaagacgc aagctttcta aataaagctc
     3121 ttggatattt tcaggtgaag gtttcattac gacttgaaat tgatgatgct gatacaaacg
     3181 gtttgggttt tctccataac gcccgtcagc tggtcggcgg gatggctcta cataagcagc
     3241 attccatggt tccggtccga ttgcccgtaa gaatgtatat gggctcatcg tacctgctcc
     3301 tttttctgtg tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa
     3361 tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt
     3421 ttaagcgttg ctgtcaataa gaatacagga aagaaaacga caaaaagtcc ctatgtctga
     3481 ttacagacat agggacgaat aatttcgcgg ttccacccta cttccgaata aagattcggc
     3541 agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggcttccact
     3601 ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt
     3661 tgcagttttc ttgtttatat aatagatcct ctcagtgaac gatctattta atctacggtt
     3721 aataatagac ggaagtccct gtatttgtca aatagttttg atttttttgt tgtatctttc
     3781 atttattctt ctttttcagg tatctctttt tcaggcatat ttttctctgg cgtctttaat
     3841 acgtgtgccc aagacttcat ttgatcaatg aactttttgc tttttagatg aataccgaca
     3901 tattcatcat acaactggtc aatgaggtca cggattgcct gcttcgtttc ttctttcaag
     3961 tggattccac tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa ataaacagcg
     4021 cgaggatctg catgataacg gtgttcgtcc attggccagt gtttctcgca tatcacaccg
     4081 ccagatttgg aagaatagtc aaattttcct tttgtttccc cgcatacgac acagtgcgtc
     4141 caaattggcg cgattccgaa gcgttgcagt atttgaactt caaagatatt cgtgatgatt
     4201 tctgcatcat ctccttgatc catccgctgc agagcttgtt gcgtaaattg gaataaatgc
     4261 gggtcatatt gatgatcttc gattgctgca tctactagat ttagcaaata ggtaccataa
     4321 ccgctcaaaa agatatcttc ttggatgttc cgaaacgcat ggacgtcttt gacactgctt
     4381 aaaaacgaca aaccttcact tcgaaaatta ccgatataga ctgcttcggt aaaggggagc
     4441 aaagccggag ctaatggctg attttgccga tggattcctt tgatataaaa catcattttg
     4501 ccagaagatt ccgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat
     4561 aaaatcaagc ctttcgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct
     4621 ggaaaagcag attctgccga tccagattat ttatgtttcg atcttccgcc gtttttctca
     4681 acgtgcggat ttttatccag tttatttcta tacttaacct ataagaagtt cagaatatca
     4741 gtattcgtct tttcggtagc caaagtcttg cagatagact tttttgtctc gccagtcttt
     4801 ttggactttg acccaaagtt ctagaaatac tttgtctcct aaaagatttt cgatatcctt
     4861 tcttgctttc gttcctactt gcttcagcat ttttccgcct ttgccgataa tgattccttt
     4921 ttggctatct ctttcgacga tgatcgtggc ctggatatgg attttatcat tctcgtttcg
     4981 cttcatcgaa tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa
     5041 gactttctca cggatcaatt cagaaacgat aaagtattca ggatgatcag tcacttgatc
     5101 atctggaaaa tattgtgggc cctctggcat ttcactgact aatacatcca ttaagcgttc
     5161 gacattgttt ccttctgtag cagagatcgg aatcacttgt gcaaaaggca tctgcgtcga
     5221 ataatcttcg atgattccca gcaactcgtc tggatggatc ttatcgattt tattgataat
     5281 cagataaact ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccggcc
     5341 gcgtttttga tcagcactga tcataaataa gactgcatcc acttctttca acgcactgta
     5401 agctgcttct accataaaat ctcctagacg atgctttggt ttatggattc ctggtgtatc
     5461 gataaaaatc agctgagctt ctggtgtagt atatactcct tggattttat ttctagttgt
     5521 ctgtgccttg tcactcataa tggcaatttt ttgacccacg attcggttca ataatgtgga
     5581 ttttcctaca ttcgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata
     5641 cgttctcctt tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca
     5701 atgatgacag caaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt
     5761 ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca ctacattttc aaaaacagta
     5821 ttgatgatct ctactatcca aacgagaaaa acagctaaaa gcagccataa ccactctatc
     5881 cgatcaagct ggaaaatcac gcccataaat aaagcaagga taccgaacgc aacatgtttc
     5941 ctcatattgc gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa ttccacagac
     6001 atcaggaaat gtttgttttt ctctacgttt ttatcttttg agtccatagg catccaagat
     6061 ctctttctgt aaaccaaaca tttctttctc atcttctggt gtcatatggt catacccgtt
     6121 gatatgcaag aatccatgta ccgctaaaaa gccaagttcc cgatcatatg aatggccgta
     6181 agcttctgct tgttcagcag cacgttcaat agaaatcatg atgtctccta gtacacgagg
     6241 caaaggattc tctccatctt caaaaatcac tgggatttcg tcttcgcctt cttcttccag
     6301 agcaaaactg atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg
     6361 gattgccgca ttgtccatga aagtaacaga catttctgtt tcctctggca gctttaaata
     6421 agatgctgca aattgcagca agtcatcgat ttctttcatt ttctcttctg agacttggtt
     6481 tgtttcatcc ataaaggtaa tatccattat acatcgcttc tttctattct tcttgttctt
     6541 cctttatctt ctcaacttct gttttcattt taggatattt gatccgagaa tggaaggtgc
     6601 tacttaaacc gctgatcaat gatttttcca cttttcggat ttctttcaag gtcagtccac
     6661 tatcgtctaa ttgaccatcg gaaatacgct cttcgataag attatggaca aaactttcga
     6721 ttttttcgct cgtcggatga tccattgccc gtactgcagc ctcacaactg tcagcaatat
     6781 tgacgatccc tgcttcacga ctttgcggtt ttggtccagg ataacggaat tgctcttctg
     6841 ttacttccgg attgcgttct ttcgctttga tgtagaaata acgcattagc gtggttccat
     6901 gatgctgttt gcaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg
     6961 ctccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt
     7021 gcgggttttc cgctcctgaa ggcaagttct caacgaaaaa gctagcatgt ttgatcttgc
     7081 cgatatcatg ataatagcaa gctactcgag ttagcaaaga acgtccgccg atttctgcta
     7141 cagcgttagc acttagatta gctaccatca tactatgatg ataagtacct ggcgcttctt
     7201 ccagcaactg tttcaataaa ggatgatttg gattgctgag ttcatttaat acaattacac
     7261 tgtcatcatt gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc aagaatgaga
     7321 agagtgcacc agtgaacccg cagacaagca ttaaccaagt cgttgcatca ccaaagctca
     7381 ttccttgata gacgatcaaa atgacatcca tcatcacagg aaaaataatg acccacatgg
     7441 cagcagataa tccttgttct gagatccgtt tgcgtctgac aatcgttgcc atgagcccag
     7501 agaaaacata agacatcaag ataatcgtta gaaagttcgt gccaatcgaa tcataaaaaa
     7561 tgaaaagtgc agatactgct tggaaaatac cagccatgat ccctgctctt cggttcaaga
     7621 aaaagcttaa aacaagtggt gcaaaagcag cgggataaaa cagcgggata taagtagctt
     7681 gttccgtttg gaataattgg aaaaatttca tcagcactat gctgactgac atagctgtca
     7741 catagaaaag cacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa
     7801 tcagtacgcc gatttgaagc aaaacagcta aaaccatggc aatgagtggg aaaatcgaag
     7861 tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca tcaatttgat
     7921 ttccttcgcg aacaatgatt tctccttgga agatcatcac tggttgtaca gcttctcgtg
     7981 cagattgttt taattcttct gtctttttct cattcaatac atcattgact acaatgcctt
     8041 gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa tactgaatct
     8101 gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt ttactcatct
     8161 gttcgtcaat gagcttcaaa ctttcatccc taaccgtgtg aatttccgtt tcagtcattt
     8221 caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg ttttctgcat
     8281 tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta ggtttcggga
     8341 tattttcatc ttccttcgcc ttatctacct tgtcattata agatttgttg atggtatcat
     8401 tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca tctgccagat
     8461 ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt ctttgttcgg
     8521 tttcttccgt gttctcgatc gttttattcg cacggatact ctcttctgct acttggcctt
     8581 ctctgaaatc attggccttt tgtttgacac tactaaatgt aacgccgcag acaatgataa
     8641 aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt agtttctgca
     8701 gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat gaagatccgc
     8761 cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc
     8821 aaaattaaca aaatcgattt ttttgatttg ctttagtgtt cgttctgcat gaacaagacc
     8881 acttgtcgtc cctcgaggca agtctatctg acttgtatcc ccattgacaa tcatttttga
     8941 ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt tttgtgcttc
     9001 gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta atggtgcgat
     9061 ttcgattacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa
     9121 agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc cgggtagaaa
     9181 gcctaggttt tcccctgcct ctacagcggg ccgcgtcaaa atgatttttt gcacttcgcc
     9241 tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg ttccggctgg
     9301 ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct tttgaccgct
     9361 gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct catacatctc
     9421 aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacga catctggtgt
     9481 acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa
     9541 ggaaatctgg gattgttcac caatgatctg aatgatttct ccacgtgtat ggatggttgt
     9601 ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac
     9661 atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca
     9721 tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca
     9781 aataagaaaa ggttgtaaaa gagtcgttta accatacgta ccaagaaacg ttaaaaaata
     9841 gctgtttcca ttcgctaaag gtctcgtttt gataaaaaga atctgagaat gtaagttata
     9901 tgaaagtgga gaacaaaata ttccgtttca ttcagaggta ttctctaggt atttttcttg
     9961 tttcaaaaaa agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa aacaggtgta
    10021 ttattacgtc aacaaataaa catacagcaa agcgtcgagg aaaacagtag gctattcaac
    10081 gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata gacgaacatc
    10141 atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat caggctagcg
    10201 gataacttta tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt
    10261 accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt tatattttaa
    10321 ggtggtggtt ttcatggaat ctgatattag tggcattact aattgctgca acgtttatat
    10381 cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca
    10441 tggaaacaac tattgatggt cagttcactt atcttcggga tgtttttcgg gtcaggtaat
    10501 ctaatctttc ctgtccactt aggtcaaatg gctggcgcaa actgggtcag tgctggcgta
    10561 ggatttgcca tctcaggcgc tcttttccca ttactagcta tcttagctgt agtagtgaca
    10621 aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc
    10681 ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac agctgctact
    10741 gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt aggtatgttt
    10801 ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc cacacggttg
    10861 gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattattagc aatcattttt
    10921 gtcgtttctt tattccaccc aatgggaaat ctaaatcaat ctgctgcaga tacttaccaa
    10981 acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc agtagccttg
    11041 cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa agataaaaaa
    11101 gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgctagaagt catgatctat
    11161 tttggtcttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc agaaaatggc
    11221 ggaactgcac tttctcagat cactaatttt tatttaggta acttcggcgc tagcttctta
    11281 ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt ttcttttgca
    11341 caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact
    11401 ttcgtctctt ttgtcgttgc aaatgctggt ttggacaata tcatccaatg gtctttacca
    11461 gtattgatgc ttttgtatcc attgtcgctt gcgttgattt tgctttcctt aacagcaaaa
    11521 tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc tgttcccgcc
    11581 gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt tgtagcaagc
    11641 atgctcgaat tttatcatca tcacgttcct tttgctgcat tgggacttgg ctggatggtc
    11701 ccaacgttac ttgggtatgc tggcagctta ctcttctatt acgcctatcg cctatcaggg
    11761 tataaacaag aagcaaatga actaccagaa gaataaccag atcaaacaca atttaacata
    11821 agaaatatta aacaggaaaa aaacagctag atgaaagaat cctattcttt cgtctagcta
    11881 ttttttagat ctctttttta cgagcacaaa aaaactgcgg acaatgattg tccgcagttt
    11941 aaatgataaa attaagattg taatagttct ttcacaatgg catttacttg gtttccatct
    12001 gcttttcctt tgactttagg catgatcgca cccatgactt tgccaaattc tttcattgat
    12061 gaagcaccag tttgcgtgat tgcttcttga acaagttgtc gaatttcttc gtcggaaagt
    12121 tgttccggca tgtatttttc gactatcaca atttcacttt ttactttttc agctaaatcg
    12181 tcacgtcctg cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac
    12241 aacaccgtta attcttcttc ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt
    12301 atagatgact tgatcattcg gatgacttgt aaagattcct tatctttcgc tttcatcgct
    12361 gttttcatgt catcgtttaa tctacttagt agtgacacac taccaactcc aattcatcac
    12421 agaactagaa ttttttgcgt tttctagctg cttcagattt tttcttgcgt cttacacttg
    12481 gtttttcgta gaattcacgt ttacgagatt cttgtaaagt acccgctttt gacacggaac
    12541 gtttgaagcg acgaagagca tcgtcaagtg attcgttttt gcgaacgact gtttttgaca
    12601 tgttaattcc ctccctccga gcttaaaaag taaccgtaat tgttattgaa atatatctca
    12661 aaaaacaaga ctgtcctgta aatattatat ctaatgctgt tttgaccgtc aaggaaatct
    12721 tgcttttttt taaaattatc aaatgttatt ttatttaaat ccttattgac aattttcaca
    12781 tcttccgaaa atctcgaatc tatgcccttc gatcgtacat ccaccgagct gttcttcgaa
    12841 ataattcatt ggacacatat ggatttcttt cgttgttccg cacaccgtac agataaagtg
    12901 atggtgatgg ccgatttccc cttgacagca atgaaaacga aacttcatct ctccatttaa
    12961 atcggtttct tccagtaaat cgatttctgc aaaatcatgg agatttcgat aaatcgtatc
    13021 gtaacttact cctttgaatt cctgattcat atagtcaaaa acttctttag cggatacgta
    13081 gcgattgcgc ttcactaaat aagttaacaa tgcttctctt tttttagtat atttaaaacc
    13141 gttatttttc aatgtttcta gtgattgttc gatcaacgtc tgttttttca tgacttattc
    13201 ctccaaatca aaatgattac gaagtacaca ttctagagta tggtataata aacataaaaa
    13261 aaatgcaagt gaggttttat ccatgtcttc cgattctaca gaaatcgtta ctttttttgt
    13321 catctccgac tctgctggag aaactgcgac aaaactagcg caagccacta tggcccaata
    13381 tcctagcgtt gagttcaatt tattccgcag aacatttgtg acagatgaga aaacattgat
    13441 gcaggcactg caagatgcac tagaagaaaa agctcttgtc ctgcacacat tgatcaatca
    13501 agaattgatc gagatgacgc gttctttttg tgaagaacat caattattta gttttgatgt
    13561 gatgactccg ccgatatcag aaatcgagcg cttaactggt atcaagccca ttcggcaacc
    13621 tggcgccctt catttattga atgagaacta tttcaaacgg atcaaagcaa tggaatttgc
    13681 tgtaaaatac gatgacggga aagatcctcg tgggtttcta gaagcagatg tcgttttgct
    13741 aggcgtatcc cgaacttcca aaacgccttt gagcttgttt ttagctaaca aaaatttaaa
    13801 agttgccaat ctacctttga tcccacaagc acatattccc aaacagcttt gggagatcga
    13861 tcctaaaaaa atcgtgggac tgacgaataa cccagatatt ttaaacaata tccgtaaaga
    13921 acggatgcgc tcttacggat taaatcctga tactgcttat tccgatatcg aaaaaatacg
    13981 agctgaactc gattttgcga atgatcttta tgaaaaattg ggctgcgtag taatcaatgt
    14041 cgcttcttta tctattgaag aaacagcttc gcttattttg aatgctttag acctagaaga
    14101 ccatagttat tatggaactg aaaccaccga agaaacagac taacaatttt tctttaaaaa
    14161 aactggactg tgtcagcgaa gaagattcgt tgacacagtc cagtttgtct ctatttgcac
    14221 ctatcgttat tgatgttcca catgtttgag tgtctggttc aaaatatcca gtacgtgaga
    14281 atcatcaaga ctatacaaaa tcgcttttcc ctcacgatgg gacttgacga catggttttc
    14341 tcttaataaa cgcagttgat gcgatacagc agattgttcc atttccactg cttggatgat
    14401 tgctgtcaca ttttgttccc cgtctttcag atacagcaga attttgagac gagtggggtc
    14461 actcaacatt ttgaatagtt ggctgacttt ctctactttt gttttttgcg tgtctggcaa
    14521 tccattcacc tccaacttga aaaaagactg acttctaaag tttaccactt tttccatcag
    14581 tctcaagttt ttttattctt ctgtcatctt ttggataatc gtttcataag cggctacttt
    14641 ttcagctgcg tcttctgaag agtttccttt tacaccgata taaaatttga ttttcggctc
    14701 agttcctgaa ggacggatag cgatccagcc attttcttcc agcgtatact tcaatacgtc
    14761 agatggcggt gtcgttagtt tttctacgtt tccttcacgg tctgttctcg tcaactcttt
    14821 gaagtcttct gtttggatga cttttgctcc ggcaaattct gttggtgctt cttcacggaa
    14881 tttttccatc aaagcagaga ttttcgcaga accttcttgt ccgctcattg tcacagaaat
    14941 cgttttttct gcgtagtagc cataggtttc aaaaatcgtt tgcaagccat catacaacgt
    15001 tttgccttgt tttttatagt aagctgctac ttctgctaat aagagcaatg cttggatcgc
    15061 gtctttgtcg cgaacgaaag gtttaactaa ataaccgtaa ctttcttcga atccgaacat
    15121 aaaagtatgg gaatgatctt cttcaaattg ttgaattttt tcagcaatga atttgaatcc
    15181 tgtcaataca ttgaacatgg tagccccata gtgttccgca attgctgtag gtaattcgct
    15241 agaaacgatc gatttcaata ccgctgcgtt ttcaggtaaa gtgcctcgcg attggtgtgc
    15301 ttccaaaata tattgagcaa acagtgctcc gatttggttt ccggtcaata cttcatagga
    15361 gccatttggc aaacgaacgg ctgcacctaa acggtctgca tcaggatctg ttgcaatcag
    15421 caaatcagcg ccttctttct cgccaagtcg tatcgcatac tcaaaagcag agtgttcttc
    15481 tggatttggt gatttgactg tagtgaaatc tgggtctgcg acagcttgtt ctggaactag
    15541 tacgaattgt tcgaatccag cttgtttcaa cgcacgttcg cccaacattt ttcctgttcc
    15601 gtgaagtggt gtgtacacaa gtttcaaatc tttccccatt gtttccacca attcatgatc
    15661 aatcgttact gctttgattt cttctagata agcggcatcc acttcttctc cgatgatcgt
    15721 gatcaatccg ctatgttcag cttcttcgtc agataatact tcaactttta acggattgct
    15781 gacttcacgg acatatttcg tcaatgcatc tgcgtctgca ggaggcattt gtcccccatc
    15841 ttctccgtag actttatatc cgttgtaagc tgctggattg tgggatgcgg ttaccatgat
    15901 tcccgcaaac gtcttcaaat gacggactgc aaaagaaagc tctggtgttg gacgtagact
    15961 ttcgaataca taagatggga tattgtgatg tgctaatgtt ttggctgctt ccatggcaaa
    16021 ttctggtgat tgatggcgtg agtcataagc gatagcgaca cctcggcgtt ttgtttcttc
    16081 gccttgtgta tccatgaaac gtgcaagtcc ttctgttgct tgtcttaccg tatagatgtt
    16141 cattcgattg atcccaggcc ctaagattcc ccgcatgcct gcagtaccga attccaacgg
    16201 tgcataaaaa gcatcttcaa gtttttcagg atcttcaccc agatcattta attgtttttt
    16261 taagttttct tctaaatttt cttcatgtac ccattgttca tacacttgtt cccaagacat
    16321 gaaaataaca cctctttctg aaatatggtt cttaataagt atagcattca cttattagat
    16381 atgaaagtat gaggaccaaa aaacgcttaa atcaagaaag gattctttta tcaagcacgg
    16441 ttgcaagtat tcgcataaat cggattcagt agatttcacg cgaaaaaaaa gagtaaaaga
    16501 acaattaaac cgttcttcca ctctttttaa acttatgcag ttgtttcttc taaagattcc
    16561 aagtatttat acacttgttg tccagcgatc ccaccatcac caacagcagt agtgatttgt
    16621 cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc ctggtatatt cgtcttcatt
    16681 tcgttgtctg ttggaatcca gccagcttcg ttcgtcaacc ctgcttctct aaatggtgca
    16741 gttaatggat ccagtccaac atagatgaat actccgtctg ctgccaaatc aaactcattg
    16801 ccggttttga cattacgtgc tttcacacct gtcactacca tttcattccc aacgatttcg
    16861 tcaacgactg tatcccacat aaaggaaatt ttttcattcg caaatgcacg gtcttggatg
    16921 atcttttgtg cacgtaattc atctctacgg tggacgatga ctacttcaga agcaaattgt
    16981 gtcaaataga tggcttcttc tactgctgag tctcctcctc cgatcactac tagacgtttg
    17041 ttacggaaaa acgcaccatc acaaactgca cagtaagaaa cgccacgtcc agcaaattct
    17101 tcctcgccag gtacgcctaa tttacggtgg atacagcctg ttgcgataat gactgttttt
    17161 ccttcgtaag ttttatcttc acaaataact tctttgtggc tgccgtgatc cttgatatct
    17221 tgcacgattc cataagcatt ttctgtccca aatcttgaaa caccttcata catcttataa
    17281 gctagatcag gacccattat tgaatcgaaa ccaggatagt tttcgatttc tgccgtattg
    17341 ttcatttggc cgccaggggc gccttgttcg atcatcaata cggataagtt tgaacgtgaa
    17401 gcatataaag cagcagtcat cccagcagg
//
LOCUS       DAYLZO010000062        17008 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.063, whole genome shotgun sequence.
ACCESSION   DAYLZO010000062 DAYLZO010000000
VERSION     DAYLZO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.063"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..127)
                     /locus_tag="ACGVJG_001816"
     CDS             complement(<1..127)
                     /locus_tag="ACGVJG_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFJ6557753.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="ACGVJG_001817"
     CDS             complement(231..542)
                     /locus_tag="ACGVJG_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFJ6557754.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="ACGVJG_001818"
     CDS             complement(544..741)
                     /locus_tag="ACGVJG_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFJ6557755.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="ACGVJG_001819"
     CDS             complement(1135..2862)
                     /locus_tag="ACGVJG_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFJ6557756.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="ACGVJG_001820"
     CDS             complement(2855..4048)
                     /locus_tag="ACGVJG_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFJ6557757.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="ACGVJG_001821"
     CDS             complement(4235..4879)
                     /locus_tag="ACGVJG_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6557758.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="ACGVJG_001822"
     CDS             complement(4973..5107)
                     /locus_tag="ACGVJG_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFJ6557759.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="ACGVJG_001823"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="ACGVJG_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFJ6557760.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="ACGVJG_001824"
     CDS             complement(7238..7711)
                     /locus_tag="ACGVJG_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFJ6557761.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="ACGVJG_001825"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="ACGVJG_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFJ6557762.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="ACGVJG_001826"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="ACGVJG_001826"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFJ6557763.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="ACGVJG_001827"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="ACGVJG_001827"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFJ6557764.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="ACGVJG_001828"
     CDS             complement(11758..12897)
                     /locus_tag="ACGVJG_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFJ6557765.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="ACGVJG_001829"
     CDS             complement(13206..13919)
                     /locus_tag="ACGVJG_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFJ6557766.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="ACGVJG_001830"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="ACGVJG_001830"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFJ6557767.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="ACGVJG_001831"
     CDS             15108..16640
                     /locus_tag="ACGVJG_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFJ6557768.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..>17008)
                     /locus_tag="ACGVJG_001832"
     CDS             complement(16831..>17008)
                     /locus_tag="ACGVJG_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFJ6557769.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3495 c         2910 g         5324 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaataca
//
LOCUS       DAYLZO010000063        16876 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.064, whole genome shotgun sequence.
ACCESSION   DAYLZO010000063 DAYLZO010000000
VERSION     DAYLZO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.064"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..1034)
                     /locus_tag="ACGVJG_001833"
     CDS             complement(<1..1034)
                     /locus_tag="ACGVJG_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFJ6557770.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADAR"
     gene            complement(1027..2340)
                     /locus_tag="ACGVJG_001834"
     CDS             complement(1027..2340)
                     /locus_tag="ACGVJG_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFJ6557771.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(2340..6281)
                     /locus_tag="ACGVJG_001835"
     CDS             complement(2340..6281)
                     /locus_tag="ACGVJG_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFJ6557772.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(6390..6848)
                     /locus_tag="ACGVJG_001836"
     CDS             complement(6390..6848)
                     /locus_tag="ACGVJG_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFJ6557773.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(7146..7664)
                     /locus_tag="ACGVJG_001837"
     CDS             complement(7146..7664)
                     /locus_tag="ACGVJG_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFJ6557774.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(7666..8282)
                     /locus_tag="ACGVJG_001838"
                     /pseudo
     CDS             complement(7666..8282)
                     /locus_tag="ACGVJG_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(8279..9067)
                     /locus_tag="ACGVJG_001839"
     CDS             complement(8279..9067)
                     /locus_tag="ACGVJG_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6557775.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(9129..10040)
                     /locus_tag="ACGVJG_001840"
     CDS             complement(9129..10040)
                     /locus_tag="ACGVJG_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6557776.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(10081..11415)
                     /locus_tag="ACGVJG_001841"
     CDS             complement(10081..11415)
                     /locus_tag="ACGVJG_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6557777.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            11623..12453
                     /locus_tag="ACGVJG_001842"
     CDS             11623..12453
                     /locus_tag="ACGVJG_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFJ6557778.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(12697..12946)
                     /locus_tag="ACGVJG_001843"
                     /pseudo
     CDS             complement(12697..12946)
                     /locus_tag="ACGVJG_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13137..13331)
                     /locus_tag="ACGVJG_001844"
     CDS             complement(13137..13331)
                     /locus_tag="ACGVJG_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557779.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(13836..14021)
                     /locus_tag="ACGVJG_001845"
     CDS             complement(13836..14021)
                     /locus_tag="ACGVJG_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557780.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(14871..15212)
                     /locus_tag="ACGVJG_001846"
     CDS             complement(14871..15212)
                     /locus_tag="ACGVJG_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFJ6557781.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(15212..15442)
                     /locus_tag="ACGVJG_001847"
     CDS             complement(15212..15442)
                     /locus_tag="ACGVJG_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFJ6557782.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            15729..16067
                     /locus_tag="ACGVJG_001848"
     CDS             15729..16067
                     /locus_tag="ACGVJG_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557783.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(16272..16834)
                     /locus_tag="ACGVJG_001849"
                     /pseudo
     CDS             complement(16272..16834)
                     /locus_tag="ACGVJG_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         4963 a         3547 c         2861 g         5505 t
ORIGIN      
        1 cgtgcatctg ctaatctacc taaagtatct actttggcta ctccattttc ttctgtcaaa
       61 atcgtcacga caaacccgtt gcttactact tctactatat aatcttgtcc ttttgttaaa
      121 atcgtctcgc cggctcgcac ttctatttcc tgatcataat aagttagacc aactgtaggc
      181 gtatctgtga ttttgtaatt tttgataaca gcgcttccgt tttcaaggac agaatcgatg
      241 aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc tagatcaata
      301 taagaaatcg tttcttgttc tccagcttca ttaataatcg ttacttgtct aaggtcgctt
      361 tcctctacat tcatctgttt atctgcttct ctgatttcgt ttttcggata caaatgcaca
      421 tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca cgccatccac catatcaggc
      481 atcatcactg gcatcataac aaccattgga gacgcgattc tggaaatatg agctggcgaa
      541 taagtttcta agaaaagata gactttatct ctagaagaag gtgcttcttc cgtcacttgg
      601 acggtagaga ataccgcttc tccagcttca ttcgttgttt gtttgtcaat acttgtcagt
      661 cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca
      721 gaattaagac cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat
      781 acttccgtta cgtcaaacaa atcaaaatcg actcctggaa tattttcttc accaaaatcc
      841 gtagaaacca atccgctatt cggtcgttct tccggaacag atgagaaacc ttttttgtgt
      901 aatgtaatcg tcacttcact tggtttttgt gcgacttctt cggctttgac tagttttccg
      961 ttcccgaaaa tccctattcc cgcagtaacg agtaacagta cactcattcc cgtgacaatc
     1021 atttttttat tcattttttt ctccccgttc tttttttatt aataaaacag cacctaacaa
     1081 taaaagaatt cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga
     1141 ccattttcct gattgacctg gattgcttgg accactcggc tctttcggtt tgtccgttgt
     1201 ttcttttacg gtctcgttat aaacacgtgg tgttgcggat tcataggcag agactggaac
     1261 tttcccggat tctaattcag ccatctcctg tccgttaact actacatatt gtggttggcc
     1321 ttttgcatcc acccaaaatt cagggatgat gacctcgatt ctcttacttg cttcatccat
     1381 ctcgtaacct tccacacctt ttaattcttc aaaataatag gtgccagaag gcaagaaacg
     1441 ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat ttgcttacat ttttgtcatt
     1501 caaaggatca ttgtctgctg gtttgatcca ttgatttttc aagtcattag caggagccat
     1561 atctaaataa tatttccccc cttctatcat ctgatataga gcaaaaacag caccttctaa
     1621 tggttttcct ttttcagttg ttcctctttg acgtccatac ttgaagaagt atggatctct
     1681 tagataaccg acatttttag gatagatatg aatctcttgc agttcctcgg ttgattctgt
     1741 cgggtgataa attggcaaaa tagcagcaat tgggatagct gtctgatcga tatcaacatc
     1801 caatccaact tctggatcaa tcgattcttc gaaaatcaaa taaactgcat cccgatcttg
     1861 gtttttctta ggtgctttga tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt
     1921 cccagttgtt accgaagtcc ctatttggac aagcctttct tctgtgatta gctgacggat
     1981 attgtttctg ttcgtttcag cgatttgctg gacgaattct ttaggagtca tgttttcagc
     2041 agcttttttt tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag
     2101 tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct tcctcagaga tagccaagcc
     2161 atcattttca tggtattcaa attgatcgtt ataatcgata tctcgaatca tccgtttatg
     2221 caacacgaat tccacgacat cttcccctga ttcttctgcc gacatcgcat gtggaagaag
     2281 gactgctgca agcggtagta aaacaagtaa ttgaagcatc aaacacaaca gctttttcat
     2341 taccttgctc cctccttttt agattgtctg acaaacaaac cataggctgc tcctgcaagc
     2401 gccatgaaga atgcagcaag caggatatag aataatcgtc cggatcctcc tgtagcaggc
     2461 aacggatttt caggatcgtt cccgacacta agctgtaatc tgattcgatt tgtctccaaa
     2521 agttcatatt ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt tggataatac
     2581 gaaatctcaa cttttagtgt acccgcttct tttcctggaa ctacccgatc attttctatg
     2641 tcaggttctt ctcgataact aatttctaaa ataaagtaac ctggaagttt tctatagctg
     2701 tcaggtgact tcacttcctt gatccgatag accccttcca acaagtctga agcattgaat
     2761 agggaagaat cgtttggatc tggtgccaag cctccctcga tgatttcata ctctcctgca
     2821 ctattttgtt tctcaattga aaattctgcg ccttcaagac gtcggctatt catactgtca
     2881 attttcaaaa gttccaaatc gattggcgct agttcatctt ctaaggttac ttgtagtaca
     2941 ccatcgatga tttcatagcc ttccggcaag ggagcagttt cgtcttcgat gaccgtatct
     3001 tcaatgataa acttaccttc ttcattcagt gcaaactgga acacttgatc agatagccga
     3061 tacccatttg gtgcgacaga ttccgagacc tcatattcac caatagacag atgcagaggt
     3121 tggtcgtaat ttaaatcgac atacttttct actcctacgg cacgagaata gaagtatccg
     3181 atcccaggca aacgtttcga ttcttctggt tctaacggat taccagtgta tgctcgatac
     3241 agcgggaagg aagaatcccc cactttcctg atatcgaaac ctgcaccatc taatgtatgg
     3301 ccttcatggt ctaattttct aactcggatc tcccgataat caaacgtatt gtaagcagtg
     3361 aatgctaccc ataaacgatc ctcttcatct gttgtatacg tgagatattg ccaattttct
     3421 tcacctgttt gcttgagttg gatagtccct atagcattat cgtaaagtcg caagatccat
     3481 gtgtcatttt catcaggttc tttgaaacct tctgggttct ggctctcaat gattttgtat
     3541 tctgcatagt tggtagtttc ttcactatcc ttataaacaa atgaatctgg ctctccgatt
     3601 ggatgaataa ctttctcacc atccacgatt acatatttcc cgtcttcatc tactgaatag
     3661 aacgtgagtc tccctttctc ttcctctact tcgttcacac cagaaagccc tttcgctact
     3721 ggatatccga tttccagtcc gttaccatct gtttcataaa gttcaaactc tgcgccattg
     3781 attggcatat tactgccttc aattagttta ttgatattca tgtggattgg cttgaattca
     3841 tttcgcattt ccagtttgac atacaactca tcatactctg tgccttctat gttaggctct
     3901 tgtacaacag aaagcttgtg atattctcct tcataatagg aattcggatc tttaccttcc
     3961 ggtccgccga ctctcgaacc agatgtatat tcatctacaa gctcgtagtg acctaacatc
     4021 tcatcttcgt tggctgcata ttttttgatt tggaattgat acgtctcatc tactaactga
     4081 taacgtggtg gtccagcgat ctctctgatc caaaatatgt cgctgactgc tccagtttca
     4141 tctcctaaaa tcggcatctt cgtttcaaag atcgctattc cgtccgtggt agttttactt
     4201 gtttcgattg gttcggcttc cccactctct ggattttggt catataatgc aaattcgact
     4261 ccagccaacg gctcgccatt ttgacccttc ttgctgactt ggaaattacg gcctttatgt
     4321 tcgtaacgaa tggaagaaga tgggaaatac atccgtccag tagtgatatc attctcataa
     4381 tcctccaggt tctgaatata ggccatattg ttcactgtgc aaaaatttcc gtcttgagct
     4441 cccgcatcaa gttttacttg gtacttgatc ctgaccattt ccccttcacc taaagaaatc
     4501 ggagaaaatt tataagcttg gttttcccgt ccgacatacg ttgtcggagt cgccactact
     4561 tctggtgggg cttgtccatg acgttcttca tcccatattt ctggctcttg gtacttcatg
     4621 ccgttttctc ctggatctag atggtaaaac tgaatatctg ccgatctgcc agctccaaat
     4681 ggtgagacat taaaaccagt ttcatcataa agtgtggctt gctggatcgt tctcttgaag
     4741 gtagaagaga tatcctccaa ggctgtaaca atgtcattct tgttgttcgc attgtaatat
     4801 cgcggtgtta cgccatcttc ttctaaatca gcaatatttt tcaatacatt tcgtccaata
     4861 gcatctagtc gttgtctccc gctaacgttt ccatcgattc ctaaaccaat ggagaagata
     4921 cttggcgggt tctcttgata cttgtttttc agccaatgat gataagcaac tgtgttgatc
     4981 gcagatgact tttcttgcga agtaggactg ccattcgtat tgttattaga agaaccatca
     5041 ccaacccagt gagtcgcttt attatctcct tcttttacct ctccataacg atagtgtaca
     5101 tttacttcat cttctggacg gttaaagtca gatgcatagg taactggatg agtaaaccct
     5161 gcattagaag tattcccaga aaactcctca aaatttctga acaaaacgtc attatcttca
     5221 gcaatcttat cttccatcac agtccagttg ctccaagctc cattagtgct cgtacgataa
     5281 cttgactgaa tcggtgcata actgaaggtc ggtgtcccat ctcccacaac gattaggatt
     5341 ttttctggat ttctattttc tccgccattg tctttataca atgtttcata gccattttta
     5401 agccctagtg taagcggagt tcctccgatt ggtgtacgat ttaatgctgt attctcactt
     5461 cccgtcaaac gattgatatc atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg
     5521 ttattgatag ctgtactatt atggtaaaaa ttcaccatac cgattcgaat atccagactc
     5581 gggtcagaga gcagatcttt actcatttcg tttactgcct caatcaacgc agcattttta
     5641 gtttgactat tggtcccagc ggttaattca ctcatactcg ctgacttatc tagaacaaaa
     5701 acgatatcga ttggactgat ttcctttttc tcgcccccga tgacatctag atagacattg
     5761 taaagggttg ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg cagaacaggt
     5821 tctacccatt ttttgatgta cgccccgtta taatccaagt aactgttttc aaaattccgt
     5881 tcatttcccc aaagctgaac ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt
     5941 gatccattat tagtagcata gtcgtaattt cgccagttcg ctcccccgtt tgtataatca
     6001 ctatcatcga tcaagttctc tccgtcatat ggcgagcctt tgtctccttg atagcgttca
     6061 ttgctttgtg tccaatttcc taacactttt gtatagtcat tcgttgtaaa cttcgggtag
     6121 cgatgctgtt cgttttcaac ttctccgcta ggatagatat attcataggg gtcttgcgta
     6181 gctgttggcc caagacgctc caaaataccg ttgaataact ggcgttgaat gcgctggagt
     6241 gcttgaccgc tgggtacgat tggttcgtcc agcttttcca tctcattttc ttcgctacgt
     6301 tgaatttcag cggaatcttc tgtatcagtt tcattttctt gtaattcttg gttatctatt
     6361 ctttcatcaa ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct
     6421 gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat
     6481 tcttgaccat cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga
     6541 cctgttccac gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa
     6601 cgtattctac cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt
     6661 tcttcttttt caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca
     6721 tcctcctttg tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac
     6781 tggaaatatg gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac
     6841 cctttcacat ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa
     6901 acaatgccca tctaattata atttaattta attttaattt tagatcaaat gatatccttt
     6961 ttagttaaaa aacaccaatt atacaacatt tattattaaa attaaattat aaaattttat
     7021 aacgttctca tttaacaaaa cttaagttat taagacaaat gaataaacaa ctacgctttc
     7081 ctcgaaacaa aaaaataaag cacaaatgcg catttacgca ctcatgcttt attcgtacaa
     7141 ttaattcaga taacggcagc gcataggatg ccaataacac ttcctaaaat agctcctcct
     7201 aaaatgtcag taggataatg taaaagtaaa aaaatcctcg aataagcgat caaacaagca
     7261 aataaaaagg ctaatattcc tgcaactgga cttaattcga acaacgcagc tactgataaa
     7321 aaggctagcg ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg
     7381 acaagccgac gatttttttc ataaggtctt ttccgtcttg tcgttttttt gataaaaagc
     7441 aaaccaacaa aaaaatagat caaactgccg ataagaatgg ctagaccata ttctctagta
     7501 tcaggtatca agactaaaac agcagcaagg agcaaccaaa taaaaccacc attagagata
     7561 ttactgataa atttcaaaaa attgattttt cgactatccg ttttccgttg cttggcgaac
     7621 ttgttgataa tactatcatc gattttagca atcaaacgca acatgttatt cctccttttt
     7681 aagaaccttt tcttgcgtac gatggataaa aagcaggact tgaaaataaa acaagaaact
     7741 gaaacctaat aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa
     7801 cgtgcctgcc aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt
     7861 aatccaagaa gcttcttttt gttccagttg aagcaagatt ttgtaactaa agagaagcgc
     7921 gcttaataga agcaaaaaca acgtagtata catcaatgta tagccaattc ccccacccca
     7981 atagcggaaa atctgataat cgatcaatag aagaagaaaa taaacacttg ctaaactatc
     8041 ttgctttcca taaagagaga agttctcacg aaactttttc cagaatagtg aaccgagctt
     8101 tttctcttca tttttttgta atgataaatc atatacagaa ctgttcgact tgcagttcct
     8161 aatgtgaaaa caggcaagac gcacaggaac catagtagct gtaaatggat aagacgtgga
     8221 atccattcaa tgatttctaa gaaataatca ttggcttttt taccaatcga tcgattcatc
     8281 atccttttga acctcctgtc aaatccatcc cttcaatgaa ataacgttgt gcaaaaaaga
     8341 acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc catcagataa ttccattgtg
     8401 tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt tcttgatctt
     8461 ggatatataa cagaggacct aagaaatcat tccaagctgc gttgaaggaa ttgatcgcaa
     8521 tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg actccaaata tacaaatgat
     8581 tcgctccgtc gatttcagca gcttcgatca attcattatt gatcgataag taaaattgac
     8641 gcatcagaaa tatattgaac gcattgccga agaacgatgg aacgatcagg ggcaaataag
     8701 tacctaccca gccgatcttg gagaataata catattgagg gatcatcgtt acaaaccccg
     8761 ggatcatcat cgtagaaaga accaaagcaa acatcagctt tttcccagga aaatcaagct
     8821 tcgcaaatcc ataagcaata aaagaatttg aaaggacatt accgatcact actagcacag
     8881 tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc gtatccagat
     8941 agttttccca tacgattttt tctgggaaaa aggtcggagg ataacgaacg atttcttcca
     9001 ttggtttcaa tgaagtaaac accatccaaa gcaatggaat gatcaaaatg attcctatta
     9061 ctgtcatgag gacataactt agtatttttt tgtttgccga ttcacactgg tctttttgaa
     9121 ataatccatc atttgccgtc acctccagaa taatagaccc acttaggtgc aagcttcaaa
     9181 ttgacaaacg tcagaaccaa cacgatcaga aacaggatca ttgacatagc catcgcataa
     9241 cccatatcaa aagctacgaa cgccttgttc cacatatgca gattataaaa aagcaaagaa
     9301 tttgcaggtc cgcctgttcc atttttcgtc atgacataag cttcttgaaa aatttggaaa
     9361 gaaccgatcg tagaggtgat cacatcgtaa aaaataatag gagtgattaa tggcaaagta
     9421 atacttttga actttcccca aacgcctgct ccatcgattt ctgctgattc atataaagat
     9481 ctaggaacat tttgtaatgt agctaagtaa agtagcattg ctcctccaga actccatatt
     9541 ttcatcaaaa tcaacgcagg tttcgtccat tgtggatcaa acagccaagc tggtccaggg
     9601 atatggaacc agcccagaaa tgtgttgatc aatcctgcag atggagaaag cagctgcatc
     9661 cataagatgt agactgctac tcctgataag actgccggta aatagaaagc tgtacggaat
     9721 acactgattc ctttgatttt ttgattgagt actgtggcta aaaacacact gaaaatcgtc
     9781 gtcagcggta cattgaagat gacgaaataa acggtgttgt ataacgattt ccaaaataaa
     9841 tcatcgtgaa cgaacattct tttgaaattt gctagaccaa tgaaattcat cttggatgtc
     9901 aaattataat cggtgaaact accaataact gaaaaaagca taggccctag cataaatacc
     9961 aagaacccga taataaaagg cgagataaat aaataggccc atcttgcttc ttttttcttt
    10021 cggatagata aacgcttcaa attttcttgc ctcctctgta cctagacagt caaaaatgga
    10081 ttattgattt tgcttgacta agttttctac tgctttttgt gcatctgcca atccttcttt
    10141 tggtgatttt gttcctaaaa gaatctggtc aatctgttca ttcactaatg tcgtgtaatc
    10201 cggagcatct aaaggaactg gtgtgatcac cgtattttta aagttttcgt ccgccatttg
    10261 atagatcatt tttcctttat ccgttaggtt gtcattattc actaaattct cattggctgt
    10321 cttgctcgcc atgatatcaa aacttttttc gccgaatttt tcttgtactt ctggagaaga
    10381 aagatatttg atgaattcat aagaggcctc aggatctttt gctccgtatg gtacttccaa
    10441 gacgaatccg ccaccccacg accaatggcc tgaaccttct tctttttctg ggagctgcat
    10501 cacgccaaaa tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat aattgatatt
    10561 ttgggcacgc atggctacta atccggaaat aaatggatca gatacaccag acccaaattc
    10621 ggcttctaat ttattgatcg tatttttccc ataataatct tgccagtctt ggatccactc
    10681 caaggcttcc actttatttt tagtatcaat cttgactttt tcatctttgt caaaccaact
    10741 agtcccgtta tctgcgttta gcgcccatac atcagcccct aaattccata atggataaaa
    10801 accgatgcgt gtaaattctt ttccttcttt tttatctagt ttacgtgctg tttcttctaa
    10861 ttcatcccaa gttgtcggta gatcttgttc actgattcca gcttctttaa atatcttttt
    10921 attataaaaa atgacttgtg tatctgtatt gaatgggatc gcataaggat caccttcata
    10981 ttctactgta tcccaaagct gcggataaaa atccttagaa gtgtcttttt ctacatattt
    11041 ggagagatta gttgcttgct gtgcttctgc tctttgggca acggaattta tgtcctgtac
    11101 aataacatcg ggtgcatttc cagctgttat tgcagataaa gatttagtcc agatgtcacc
    11161 ccatggctga tacttataat ttacatggat ctcgttttgt gattgattga aatcctcaat
    11221 gatttcttcg atagcttctt ttcgtgcacc ggatccccaa aaggaccaga aatcaatatt
    11281 ttttcttcca tcacttgttt tttcagaaga atttgctgca cttgaactgg catcgcttgc
    11341 attacttgat ccgcatcctc ctaataaaaa caatgctgaa cttgccagca accctgtaaa
    11401 caataatttt ttcatatttt acctccaaat tttaatgtaa ccgatgccgg ttttaatata
    11461 acgatatcat tttgttctga aaataacaat ttataaggta tgattctaaa aaaattatta
    11521 atgataccaa tactttatat aattcgcgag cgctttcttt gaatttttta atctttcgta
    11581 gcagaatgag aatgtattaa aaatagatta ggaggaattt tgatgtttag acaccagaaa
    11641 gaattgcagt ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa atatctgcag
    11701 gagctgatcg gcggccaata tggcgagatg agtgtagcta tgcagtacct gttccaaggc
    11761 tggagctgcc gaggagaaga aaaatataaa gatatgctga tggacattgc aacagaagaa
    11821 atcggccacg tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg tgcgcctgtt
    11881 gaagatcaag aagcagcgat gcaaaaagat ccaacaatcg gtgccattat ctctggaatg
    11941 aatccacagc acgcgatcgt agctggctta ggacctcgtc ctgctgacag cgtgggcaat
    12001 ccatggagcg gtgcctatat cattgcgagt ggcaacttgt tagcagactt tagagctaat
    12061 ttaaatgccg aatcacaagg aagacttcaa gtagcacgta tttacgaaat gatcgatgat
    12121 cgtggcgtaa aagatatgct gtctgttctc cttgcaagag atagctatca tcaaaatcta
    12181 tgggaatgtg cagtgaaaga attagaagaa aaagaaggtt caatcttagt cccaagtact
    12241 ttcccgcgtg acaaagaacg aaaagacatt gcttatgact tctataactt ctcagaggga
    12301 gaccaaagta gtgaaggttc ttgggctagt ggcaaagcac tggatggcga aggcgaatat
    12361 caatggttga aagaacctaa gatcgaaggc gctgcaccaa aattagaacc agtaacacca
    12421 aaaatgtatg ggacacctcc attcaagaaa taaatatcta cccgtagaaa cacccgtttt
    12481 ttgttccaca cgctttgtga gcaaaactga ctaaagtcat taatcataac cacccttcaa
    12541 aagggtggtt ttctctagag ctataagctc aaatcaccgg ccagcgccta aagacgctgg
    12601 ctttcatatt ttatgttcaa gccatattgc ctttgccagt tttgcccacc tcttaaaagg
    12661 tttatgtact actccttagc ccttaaaagg atcgtcttac tctcgtacac tcagcttatc
    12721 taacgcacta tcatgttttt cttgttcttg aatgtacttt ttgatggtcg cttcattttg
    12781 tcctactttt gtcacatagt atccttccac ccaaaagtgt cgatacccaa atttatattt
    12841 tagatttgca caatctcttg atgatctcct gaatattggc tcgatattga ttataaatta
    12901 tttttcttct atactttggt gtgaatacaa tgtggtattt acacatctac tttgtacggg
    12961 ctaaactatt tccctttatt gctccacccg catagcaagt ggttttgtat ttcaatcgct
    13021 cggcgcgctc aattaggtct aaagacaata aatctaaaaa cccgctgctt cactgacaga
    13081 aacaacgggt tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa tctattttat
    13141 ttttttgatt ttttataaat ggaaaatgct tcaagaagtc caagaaataa agccgtataa
    13201 aaacaagaac ctatgaaatc tttatttatt agatattcaa cagaaatccc gataacacta
    13261 gccaacaata tatttatcag aagtgtaaaa taaggatgct ttttatagaa tagaactagt
    13321 gttgttttca tagactctcc tttcttcata tttccaatta atcacaaatt cacggcatta
    13381 ctagaaaaat taaaggggcg cagaagtgga aaaatactct tcgactttcc ctacaaacca
    13441 ggccatcttg tgtttaataa ctataccaaa tctaaggtac ctctaaatct aaattccatt
    13501 tgtggcggtg acctccaatt tttaactctt ctatctccca tccagtatac ataaccttga
    13561 tacttcgacc acttatatat cgaacacaca aatacctatc cattgcagga gtactaccta
    13621 acttagttgc tacttgcatc taaataacag ctgaaaagat aggacttact cttagtaaag
    13681 tatctgaact attaattttt aaactatcat ttttatcctc aactacatgc acatctgttg
    13741 caacttcact ttctgtctca tccagataaa tgattaagga atcgggaatt gcaatatctt
    13801 cagctgacgc tggaatagtt gagctaaatt agtcatcata tctttattat aaaataacca
    13861 acagcaaaaa atacaatgaa aaacaaactt ggaactaatg tatcattaat aaatgttgaa
    13921 aaattcagca actcattact gtatgatttt agcagcccaa ccaacatata ccctataaaa
    13981 gctaataaga tggttaggaa tcctttagaa tttttactca ttcatagaca cttccttaaa
    14041 taacttttat tatagaaggt attgtaaata ataatattga caaaaagtga ctcaaaataa
    14101 aaaaaacctt acattatact ggaatgaact gtattgatag tgtacctaaa agctctactg
    14161 acataagatc gtgtaatcgg taaagtaatt ttattaatta tacgggatgt aatcaattag
    14221 agaataaact aatcataaaa ggtactttta cattcataat tgtcgctttg agattctact
    14281 ttggttcact atctgaccca attgcacatt ttcgatcatt acaagtattg gtgtcgttag
    14341 aacaactaaa taaaaactag cggtagattt ttcagaatct atgctaattt ctgtatggat
    14401 gttccagtga tggcaaattt ctcttttcat tgtttttctt gtcgcatgat atgccttttt
    14461 gtgttattca gttaaactat agaaattctt tcctagacag atcttgatcc caaattttac
    14521 gtttttgagc atcgatatag tttaaaatga cattactcat atctaaagag tttagatttt
    14581 ttaggactaa aactgtcagt tgttcttttt cttctttctg ttaaagtatt gtcgcgactt
    14641 ttcgtatacg ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga
    14701 cttgaggaaa tcacctcaat cattcgttta aaatgctata tattctttag tttaagatac
    14761 ggaaattaat gccatcgacc acactagtag aatgaacgac atttacttgg ccttttttta
    14821 atttcgttat gtttagcata taaaaatatt atagagttta cttacaatgc ttaaaacata
    14881 tactgtataa tctgatcaat tttagccatg tcttgcaatg gtaagttttc tatttttttg
    14941 agttttcgtt ccttaaaatc aagagatttt aattgtgtaa taattacttg tccttttata
    15001 tctagctctt caggtaatga gtaacgtgtc ggtaaattct ttatagtgga agtaattgga
    15061 catatgaccg aaaacattgt tttacggtta aattcataac gagagacgac tagtcctggt
    15121 cgtctttttt gaatttcttt tcctacggag ggatcaaaat caatccatac aatatcacct
    15181 tttttaggaa tataaattct ttcttctgac actataaaat ttctctccct tcaggagtta
    15241 aatcttccca ttcgtctttc tcataatact cggcctcttc cccaccacta aatggatctt
    15301 caatttttgg aactaacgta attgttccat catcagaata cacaacgata tattcttggt
    15361 tttctgaagg tttctttccg ttatcagaag gtaaagttac cactacagaa cttccttgta
    15421 atctagattt tgtaacaagc attatcatca ctcccttaat aaaagtatac tatataacac
    15481 taagtaacgc aaaattcccc tttgtaaact atctagaaaa ttgttttgtt taaagtatat
    15541 cttattggat ataaatagaa ataaattgta gccagttcat tttttcttta actaaaaaaa
    15601 taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat agctaacccc
    15661 ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag acaacaggcc
    15721 atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct gacaaatttc
    15781 aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg atcaaagaat
    15841 gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac tttgctattg
    15901 tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat ccaactcata
    15961 tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca actgccaacc
    16021 agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga taaacatcgt
    16081 taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc tatacattag
    16141 aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt tcttctgttc
    16201 tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa
    16261 aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt aatgattttt
    16321 cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc
    16381 attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat gattgattct
    16441 gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat tttttcctta
    16501 tttgaaaaca taaatttgtc tttttcttca ttccactcat tgggaaaaca caacgatttt
    16561 gcaaaaatgg ttgatgaaag gtcttttttt cttctactgt ctctgcacgg gcattaatca
    16621 tcaattgtcc ttttttaaat ccggtaaatc cccacttcat gatttccgga gtgaggatgt
    16681 tttcttcctc ttttcttaac gtcacaacaa gatttgacgg aaaaacttct ccagctgcta
    16741 attctgctgc ctgcttcggt tgtttcgtct gggcttctcg ataataattc tgaaggagct
    16801 ctgactctag atcaaaaaaa taacgaccac acatcctaat ccctcccaat ctttttttct
    16861 atcatacact gcaaca
//
LOCUS       DAYLZO010000064        16849 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.065, whole genome shotgun sequence.
ACCESSION   DAYLZO010000064 DAYLZO010000000
VERSION     DAYLZO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.065"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1269
                     /locus_tag="ACGVJG_001850"
     CDS             <1..1269
                     /locus_tag="ACGVJG_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6557784.1"
                     /translation="WGTDVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNE
                     IIVNPGKTIEYYCEKISISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQL
                     RIFVTFFFLSYYGRRTLPFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVH
                     LMRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSE
                     FSEKEKNEISEQIRKNMSYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTI
                     RATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTS
                     DKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQ
                     PILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(1342..1989)
                     /locus_tag="ACGVJG_001851"
     CDS             complement(1342..1989)
                     /locus_tag="ACGVJG_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6557785.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1997..2683)
                     /locus_tag="ACGVJG_001852"
     CDS             complement(1997..2683)
                     /locus_tag="ACGVJG_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557786.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2689..3762)
                     /locus_tag="ACGVJG_001853"
     CDS             complement(2689..3762)
                     /locus_tag="ACGVJG_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6557787.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3984..4658
                     /locus_tag="ACGVJG_001854"
     CDS             3984..4658
                     /locus_tag="ACGVJG_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFJ6557788.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4663..6003
                     /locus_tag="ACGVJG_001855"
     CDS             4663..6003
                     /locus_tag="ACGVJG_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFJ6557789.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6152..7138)
                     /locus_tag="ACGVJG_001856"
     CDS             complement(6152..7138)
                     /locus_tag="ACGVJG_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFJ6557790.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            7862..8893
                     /locus_tag="ACGVJG_001857"
     CDS             7862..8893
                     /locus_tag="ACGVJG_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6557791.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            9002..10534
                     /locus_tag="ACGVJG_001858"
     CDS             9002..10534
                     /locus_tag="ACGVJG_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFJ6557792.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            10673..11485
                     /locus_tag="ACGVJG_001859"
     CDS             10673..11485
                     /locus_tag="ACGVJG_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFJ6557793.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            11475..11960
                     /locus_tag="ACGVJG_001860"
     CDS             11475..11960
                     /locus_tag="ACGVJG_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFJ6557794.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            11983..12528
                     /locus_tag="ACGVJG_001861"
     CDS             11983..12528
                     /locus_tag="ACGVJG_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFJ6557795.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(12601..14244)
                     /locus_tag="ACGVJG_001862"
     CDS             complement(12601..14244)
                     /locus_tag="ACGVJG_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFJ6557796.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(14373..16601)
                     /locus_tag="ACGVJG_001863"
     CDS             complement(14373..16601)
                     /locus_tag="ACGVJG_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6557797.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(16634..>16849)
                     /locus_tag="ACGVJG_001864"
     CDS             complement(16634..>16849)
                     /locus_tag="ACGVJG_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFJ6557798.1"
                     /translation="DAYRYEKFYDPENPVVDIIGLQGNRMTVEVDTKNPKIDEDIILY
                     YDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT         5028 a         3187 c         3045 g         5589 t
ORIGIN      
        1 tggggaacgg atgtaacgat tcaatcttca caaaaattgg ggtatcgtct tgaacatttg
       61 aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc aaagttctac aaacagtcgt
      121 ttattaaatg agattattgt caatccgggt aaaacgattg agtattattg tgaaaaaatt
      181 tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat gcaatcaaat tttagaaagc
      241 tatttattaa aagtgatcgt tgaccaaggt tttcagttaa ctagccttaa aagtgagctc
      301 cagttgcgta tctttgttac ctttttcttt ttaagttact acggaagacg cacgttgcca
      361 tttccaatcg aaaaaaagaa gatcaaagaa attatggtca agaataattg tcagatgaat
      421 ttgtttcagg aagaaagccc atatgagcaa tctttcttta ttatgtattt tgttgtgcat
      481 ctcatgagag aatcccaagg aactccattt gaatggaaag ctggagactc ttatgtacag
      541 ttgataaata tcgataaaaa agacttctta atcttgaaaa atctcttagg aaaattgaag
      601 tacagcagct atataaaagt agtgaactat tttgtcaata cgattttttc agaattttcg
      661 gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa atatgtccta ctgttcgttt
      721 tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg ttcaattatt aacgcaaatt
      781 tattttttct cccgtatttt cccatacgat acgactcatt tgacgatccg tgcaacttac
      841 tttgcacagc agatgcaaca ccgccagata cgtctatatg ctggatttcg tgcagaactt
      901 caattttatt ccactctact gaatcagaat ctgatttatt atttatctag ctatatcttt
      961 tggatgctta tcggtaatcc tgaaattcac cattatactt ctgataaaaa aatactgatt
     1021 atcagcgact taagtcttcg acatagtcaa tatattgaag aatatatcag tgatattctt
     1081 gctgtgcata aaattcactc tgagacaact gctattactg aagatcagct gtcgaaatac
     1141 aatcttctcg agtatgatct gatcgttacg aatcaaccca ttttgaatgc ccatgttcct
     1201 catatattga ttgatgatag tgtgtcattc gcaaatgaag aagaactcat ccgcttattc
     1261 gagttatgaa taatcaaaaa acagctatgt ataaaccacc ctgataaaaa tttcaggaca
     1321 aatacacata gctgtttctt tttattgtat tccttcagca atccaacgag atttatagcg
     1381 aaagtctttt aacaactctt cttttgtcca attatttgca atgagatccg tcaaagtgtt
     1441 cattacgagg gtcatggaaa agtataggaa actaactgct tcttccgacg attgaatgga
     1501 gaaatgattg attcttaata attccagcca tttttcgttc attggagaat cagccgatag
     1561 ttctctacgt ttggttgtct gctgatcatt tcgctgggtt aacaagtata acctttgcca
     1621 atagatactt tcgtgatcta agtcgcagca ccactctaaa tatttttcga tccccaaaaa
     1681 gaaatgttgt ttgttttcgc taatgaattt taagatatct tgatggacta tgtgagaact
     1741 gcttttgatc gtatacaaat aagcatcttc caaatcttga aaatatttat aaaaagcacc
     1801 gcgcgacatc cccatcttgt caacgatttc tgagactttg acttggctga tgtgctgtga
     1861 gcaaaatatc tccagtaata ggtcatcgat tctctgtttc tttgcgttcg ataaattgaa
     1921 aaaagtttct tttggcatcc acatctctcc tttatctctg attatacgtg gatagccctt
     1981 cattgtcatg aaggattcat tccttctttt tctcttcttc tgtcaagtac ccatcttcca
     2041 ttcgataaac gcgatcactc cattgaatca tacggacatc atgagtaacc ataatagtcg
     2101 ctttttgttt ctcatgagct tctttaacta gtaatttgac gacttcatat gcatgatctg
     2161 tatccaaact agctgttggt tcgtctgcta agatcaagct tggctcatta tataaagcgc
     2221 gagcaattgc cactctctgt ctttctccac cagaaaggtc tttaggataa ctatttttaa
     2281 gtgactggat atctaaagat tgcagcaagg aatcgatttt tgttgaatct tctttttttg
     2341 aagtaacttg gtcaactagt tcaaattgtt cctttaccgt taaaaaagga atcaaattag
     2401 agctttggag aataaatcct atttctttaa agcgtaaatc tgctcgtttt ttttctggaa
     2461 gctcagttag tgagtgaccg ttgatcacta tttctccgga agtcggtgtt tgcaatcctc
     2521 ctgaaatggt aaggaaagta cttttacctg aaccagaagg accaatgatg ctgacgaatt
     2581 ctccttcctt tacataaaaa tcaattgctt tcaaagctga gacctttgta tgaccagaac
     2641 caaatgtttt ggtaactttt ttcatcgtta atattttttt catttttatt aacctcctat
     2701 tgctgtaatt gggtctactt tggttatcgt acgtatcgaa aacaatccgc ctagcattgc
     2761 gacaatgatc aaaatcccac tatatagcaa ccattgtgac caaaaaacag caaaaggcat
     2821 cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa agagcaagca gaacgcctac
     2881 caccccaaca ataaaggact gtgctactaa agatttagcg atgaagaaat ttttgatccc
     2941 ttgtgctttc attactccaa aaatagcagt cttctgaagt gtcatcacgt acatgaaaat
     3001 accgaccacg gcagcaacca ctaagaacaa aaagtatatc atcgcattta aggtcatatt
     3061 ttgggcagaa tagccaggaa tactttcaat gaatgtccca atagttaata actgtagacc
     3121 cttcgatgct ttttctttcg aaattttagc tgaatccttt attacgatcc cattgatcgg
     3181 ctgttcttct tcagaagcaa acggctgttg tccatacttc aaagcagtcc acgtatctag
     3241 ggacgcatag atgacgggag aaactgtgta ataagtttca ggaaagatac cgacaatcgt
     3301 caaaggatcg tcgtaactgc caattttgat cgtatctcca attttatagc ctgaatcagc
     3361 cagattttga gagatgatga tttcattctt tgttttaaat tcatgccctt tcgtgagttt
     3421 tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta acattttctt tgttttttcc
     3481 tttgattgct ccgctgtaca atccgattgg tgctttctct cctccttcaa tgtatttcag
     3541 atctcctctt gttaattgtg aagctgcaaa tacttgattt gcatcctccg acaaaacaat
     3601 ctcttgtgcg tcccaatcat cgattgcttt tttaaactct tctgaaagtc cattcgctag
     3661 tcctgacaac atgaatacga catacgcaat caagaacata ataccgacga tcaagccata
     3721 tcgtaattta gaatagcgaa tttctttgat agctagaaac atttgttttt cctccctatg
     3781 atagattgat agtgacaaaa tgtcacttgt tgaatagtgt aactctaaag tgacaatttg
     3841 tcacttgtgt tgtttttttc ttatcaataa attgtctaag acatctttcg ataaaacctt
     3901 aacatgagtc ataaaaaatt tatttaagcc ttatttatgc tttccatgat aaactaaagt
     3961 gaccaaatca atgaggtggg aaaatgatta aactattagt agtagaagac gataaagtat
     4021 tatccaataa tataaaagaa atactcgctg caatcggcga gataacacaa gtttatgatg
     4081 gtagtgaagg actgttcgag ttggaaagtg gagtttatga tcttgcaatc ttggatttga
     4141 tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat acggaaagaa aaaatacaga
     4201 ctccagtatt gattttgact gcaaaagata gtttggaaga taagattgaa ggatttgaac
     4261 gaggtgcaga tgattatcta acaaaacctt tctatcgaga agaactgatc atgcgcgtga
     4321 aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa tgttctgaag tataaggata
     4381 tcagttgtga tttaaaaagc aatgaagtct tgtataaaga acaagttttg cccatacaag
     4441 gaaaagagtt tgaattgctc gtttattttt tacaaaataa gggaatcatt ttgacaaaag
     4501 agcagatatt tgaccggatc tggggatttg attctgatac gacgattaca gttattgaag
     4561 tttatatgag caacttacga aaacatctgc gaagtactgg attggataaa gaaatcaaaa
     4621 ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg caatgaatgg agatattaca
     4681 cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag catttgcttt tatctttttt
     4741 cttttgggat tcattacttt acagatatta aaccaatctg catacagaga aacagatctg
     4801 tcattagaga aaataaattt tgattctcga acagtacaat tagagataca aagatatcag
     4861 caaggcgatc cctttttaaa cccgactcaa acggaacaaa acagaacaaa catggaacag
     4921 ccattaatgg aaccatcaaa tgaccgtttt aacactcaag tgatcttgtg gtctaaaaat
     4981 ggtgagatat tgaataaagc agctattggt gggcgtttca accaaatcaa agaattaaaa
     5041 atggatacga aaaatctgaa taccatccaa aatattgaat taaatgaaga cagtggctca
     5101 tctgccctct ctttccgttc gattacgaaa aaagcggaga cgaatcaaga tggcgttgcc
     5161 tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt cattagcgac ttttcgcacg
     5221 attatgattt tgtgtatgat tgttttttgg attctctcta ttggtatcag ttattacttg
     5281 tctagtctaa gcatgcgccc tattattctt tcttggagaa gacaaaaaga atttgttgag
     5341 aatgcttctc atgaattgcg cacaccgtta acgattattc aaaatagttt ggaacatctt
     5401 tttaccaagc ctaatcatac tattattgaa gaatccgaaa gcatcgctca agcattgtca
     5461 gagacgaggc gcctcactgg attgacttcg gatttgctga caatcgctcg gaatgattct
     5521 gatcagcagc tgctttcaaa gcaaatgatc aatacacaag aatatatcga acaacttgtt
     5581 aaaccatttc aagagatggc catcatagat ggaaaagagt ttattctcca taacgatgca
     5641 acattagaag taccaattga tgaaaaaaaa atccatcaag ttttggttat tttgctggat
     5701 aatgcattaa aatatacgaa tccagaagat aagatcattt tggattcatc tgttaaccat
     5761 aaagaatggc tgattcacgt aaagaatact ggcccttcta tcagtgaaga agatcaagca
     5821 aggatttttg atcgatttta tcgggaagat caatctcggg cgaaagagac tggcggatac
     5881 gggcttggct tggcaattgc aaaacaggta atcgaacagc atcatggaca gatttctgta
     5941 tccaattatc ttccacaagg agtcgatttt aaagtccaat tgccgttgaa gcctaaaaaa
     6001 taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg tcacagccct tttttgttgc
     6061 gaataaacgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc tttaagctga
     6121 ccacttctgt cacaacctct ttagcttacg cttacgattt tttattgcgg tcttcaatgt
     6181 gtacatcacc gcttgtgtca atatccaatt cttctcggct aacatcttct gtcacttttt
     6241 caacatcttc atgttcttct ttatgaatcc caacttcttc acgaataact gtatgtttgc
     6301 ttacatctac ttgctcttct ttgattggga tagtaatcgt gtcatcttcc aatttttcgt
     6361 ctgttccctg tgaagattga tctgttactg gtttgcgttc gatgacgatt tcttcacgtt
     6421 tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg tttgtggatg tcgacttctc
     6481 cagttgtcac atcatgtgta tttacatcta atttttcttc ttttagttga attgtatcat
     6541 catctagatt tggtttgcga tcgatgtctt cagtcttgct tgtacccatt gtatcagtct
     6601 cgccaggtac gtccatcgga tcagttcctg gtgtcatatt caatggatcc gttgtatttt
     6661 gaaagcctgt cgtgccacct acgtccattg gatctgttcc agtgccagtt tctaatccag
     6721 tccccattgt gcttgctggg ctaacagtat cttgtgtatc cattgtatct tctgttgttt
     6781 ctggctgacg atagcctttt actgtgatga caaattttcc atcagaaata tctcctctat
     6841 attgatataa aggatcattt tctggtgtag ccgtctcaga atcataggta tcaaaagaaa
     6901 aagtatcttt tattttttcc cataaagaac ggtcttcttc accatccatc cgattttcat
     6961 ctgtttcaac atctatacca gacaatccgt caaatcctag tctagatgct gcttcatcag
     7021 tagtataaat aacgatatca tctcgttcat acccttcgtt acgcaaccgt tcaattactg
     7081 ataatgcttc ttccctcgta ctatagcttc cgatgaccgt taaatttttc tcttccatgt
     7141 aaattcctcc tagcaaatag atttatgatt ggtatattgt tacttataaa tttattctat
     7201 aggagacgat tgttttctac aaatatttag attggaattt gattaaaaca atttaattga
     7261 aatttgaaaa catttatgtt aatgaaagtc atattctaat aaaatgtcat ttgtttctat
     7321 atgaattttt atgaaaaatt tcatataatt tccactcgtt gtgatggttt aatttatcca
     7381 atccatcaat aagcaaacaa cttatataaa aaaggattaa ttctcaatat aattgctcct
     7441 accatagaat aaatcttcat gtaaagtaat gaagtaccaa ctatttctaa tacttcttta
     7501 agaattatcc caacaatatc gttagacaaa ccattattga ataaatggag agccaatcga
     7561 ttctgaaaaa atgttttcta atttttcgga aataaaagaa agagcctcct gccattagaa
     7621 cgaaccaaat ccatttcatt gctcatgaag taaccacata gctaaaaaga ggataaatca
     7681 attaaaagat caaaagaatg actgcactaa taaaataact aaaaatgtat cttcttactt
     7741 catatctttt agctagtact tagttaaaaa tttgcttcct aagatcaaga caaactgtct
     7801 atcttcattt gatgaaatgc ggtaaaatat agaaaatgaa cactattgga gggaatttgt
     7861 aatggacagc aaaactagaa aacaactgca agaaaagtta aagcaacgta cacacttgct
     7921 cccttccgtc tttcaacagt ttttagaaga aaatcgttct tccctttcat taggcagtcg
     7981 ctatgaatac gcaaaagatt ttcatctgtt tactgattat ttgagaaaag tattttctga
     8041 agaagtggat gatcagctga ttatccattt agaaaaacaa gattatcaga atttcttagc
     8101 taaaatctat cgacatactc gttcgttcca aactacagcg aaccaagaaa cggagcaatt
     8161 gtttgaaaat agctattacg ggatttcaag gaagcaatat tcattaaatc attttttacg
     8221 atttttgtat caaaaaggac tgattgaaga agagatttct cttttgggta caagtttacc
     8281 ggaaacaacc aaagcagctc cgcgatatat cgatgcagaa cttttcaaag attttgagca
     8341 tttgtttatc ccgattgaag gctttcaaac aagcagagaa gctagctttg aagaaaagaa
     8401 tagaccgagg aaccttgcaa ttactgccat tcttctgtat tgcggattaa agatccatga
     8461 agtagtagaa cttaaacgta aagatgtatc tttgaccaag cagattctta atctgaatag
     8521 aaaagaaaac agactgaata aagtaccaat accaaaagaa gcgatgccat ttctggagaa
     8581 ctattttaag ttaacagcac aaaatacgga cgaaaattct tttgtttttc gttcctcaca
     8641 tgatcagcaa atttctcctc ggactattcg ccaaattttg agcaaaatta cagtatataa
     8701 aaacgtatcg ccagaactat gcagaaaaac atatcgttat tatgcagaac tatatttaga
     8761 tgatgctgac gcggtaaatt atttatgtgg taatcgatac ctctcatccc attcttttca
     8821 agatatctgg gaaaagatgg ttgatttttc ttatcacgaa ttagcggcat tcgttcgggt
     8881 ttcttccata taaaacaaat gtatgatctt tttttcacaa taaactatgt tattatataa
     8941 aggattatgt tacgattatc ttgaatttgt atattactta acaaaagata atggagtgtg
     9001 aatgatggag accataacgt attccccttt gaacttattt acgaattttc aagaagctgc
     9061 agctaacttt tccgagaccc cgattatctt tgatgagcca ctttcagctt tcccagaatt
     9121 aggacttgaa acaacttaca aaaaatgctg cgaaagtgtg ttgaaacggg cttatcaatt
     9181 ggctcatctt ggagtgaaat ccggagacaa agttattatt tataagagtt ccgcttttga
     9241 cacatacctt ctagctgttt ctgtgtctta ccttggtgca gtgcctgtca tgacttctta
     9301 tcatctacca acaacaacaa tggaagtatt tatcgatcgt ttagaagacc ctttcatttt
     9361 atttgatgat gagacaaaaa agcgagtgag agaaatttca aatggaacaa aatcaaaaca
     9421 gattcctatt ctttatttaa tggaacaacc tgctttgtct gtttctcaat catttttaga
     9481 caaaaatgaa atttcttata tgacccatac ttctggtaca actggtatcc ctaaacttat
     9541 ttgtcattct gctcattcta tgggatggcg gacgaaatgg caaaaaacag ttttcacaaa
     9601 aatttcggaa aagaaattaa tcgcttttca catctctcca gtgcattcca gattcaatat
     9661 tggtatctct tctttgatgt ccatgggatt tcctatgatg cctttagcaa acgcacaaag
     9721 ttcaaaagtt gttcatatgt tggaagctca ccggccaatt gcactagaga cccatccgaa
     9781 taactttgtt cagtggcgtt ttactgctaa ggagcatcca gaagcttttt cgggtatccg
     9841 atactatcac tccacttttg atgctataaa caaccagaca atgaagacat ttttgcgaac
     9901 gtcgatggca caggatgcgg tatttcttca agtatatggc caaagtgaat gcggaccaat
     9961 gattcttaag gctcatacac tagagagttt gaaaacaagt gatgcaagag atatgggcgt
    10021 tggactggaa gacatgacaa gcgctcggat tacagattca gttgggaatg tcctacctga
    10081 aaacacagat ggtcatatac aattactgtc taaaggacgg gctttgactt attacaagga
    10141 agatgcacgg ttccaggaaa atgtttatgg cgattggtgg gatagcggag attacggttt
    10201 tatggataag catggccacc tatttttgaa agatcggcaa gtggatttga tcgaaacgat
    10261 caatagtaca ttagctattg aagacttttt attagattcg ttagattttt tggcagaagt
    10321 agtgattgta agggataaaa atcattctcc acaaccgatt attgctttag ctcctgataa
    10381 agaaatggat tggaatcgat ggtgggagca agtccatgaa cttcctcgct tgaacgtccc
    10441 tatcatccgt gattttgatg caatcccgcg aacagctaca atgaaagtgc aacggcttca
    10501 aatcgagaaa gaattaaaat cacaaaagtc ctaactattt aaggaaagaa agatccattt
    10561 tggtctttct tttctttttg tttttttaaa ttggatgaga taaaaatatg ccaattggct
    10621 gtttttcttt tttgaaaatt tcgttatact cttattcatg gaggaaaatg ttatgaaaag
    10681 aatcttgaca attggcggtt ctgatccatt tgctggtgga ggaatccaaa cagatttaaa
    10741 aacttttgag aattttggtc tattcggact aagcgccttg acttctatag gggcactaga
    10801 tattaatgag cgatttatgc tggaatctat atctttggaa ttgcttaaac gacagttagt
    10861 ttcgattgat aaaatgactt cattagatgg tatcaaaatt gggttattga attcaagtga
    10921 agcaatcatg gttgttcgag acttcataaa aacaaaacaa aatatcccaa tcattttaga
    10981 ccctgttttg gcattcaaag aaactgaagc tgtgatcaat caattgtata tttcgcagct
    11041 catcgaagaa ctttttccac ttgttacgat gattacgcct aatttggcag aagctgcttt
    11101 actttctggt caagctcttc cctcttcgtt aacagaaatg atccatctcg ctaaagaact
    11161 cactgcttca ggaacaaaaa acattgtgat caaaggcggc acaaggatgg ctggagatga
    11221 agctgtggat cttctctatt caattgatca ttatgaggta ttttctcata aaaaaatcga
    11281 cactacaaca gtaaacgggg ccggttgtgc cttttcttct gcaataacag cgaatcttgt
    11341 gttaggggca gattttacca aagcagtagc agaaagtaaa aaatttgttg cccattgtat
    11401 ttctaatggc gtgctgatga acgatcatac cggaagtgtc tggtctggcg gtattcaaaa
    11461 aggaggaatg agaaatgaaa actaagcaat taacgatcta tgctatgctg attgcgttga
    11521 cagttgccct ttcattaaca attttgatcc ctgtaccggc tacgaatgga tttgtgacat
    11581 tatgcgaggc cgggatctat accgctgctt ttctttatgg tccaacgggt ggattagttg
    11641 ttggtgcttc cagtggtcta ttgatcgatt tgatttcagg atatccgcag tgggcagttt
    11701 tttcattagt gatccacggg ttacaaggat atgttgctgg ttctttcagc aaatcttcta
    11761 ctcgtatgtg gggattaggg cttttattag gaagcttagt tatgatcatc ggctatggta
    11821 tcgccggttg gattttatat gattggccgg ctggagtggc ttctatacca ggaaacatct
    11881 tccaaaatct gattggaatg ggacttgcct ttcctataac tgcaagtctt caacgtattc
    11941 aagcacggaa gcaatattaa ttgaaaaaga aggttttaga aaatgaagtt gtcagaaaaa
    12001 gaattaaaag accaattgac tgaaatagtt aatgacatat tggctgaggc acacttgaaa
    12061 aaaggtgatc tttttgtttt aggatgcagc acaagcgaag tcgttggagg acatattgga
    12121 aaaaattcca gtgcggaagt tggtcaatgg atcatccgta cgttaaaaga gttgttggat
    12181 cctaaagaaa ttgcgctagc tgtccaaggt tgtgaacatt tgaaccgagc attagtagta
    12241 gaacgaacag tggcagaagc aaaaaatttc gaaattgtct ctgttgttcc tgcactacat
    12301 gctggaggtg cttgttctgt tgctgctttt gaccagttca atgatcctgt cgaagtggaa
    12361 cacgtagttg gacaagccgg tatcgatatc ggtgacacat caatcggtat gcatatcaag
    12421 catgtccaag tacctgtcag acctcgtttg aaaacccttg gacaagctca cgtgacagcc
    12481 cttcgctctc gtcctaaata tatcggtggt cctagagcga attattgaaa tgagtaattg
    12541 acagagcaat ctgaaaaact tcctcgcaaa gtccaactaa tgaactttaa cgaggaagtt
    12601 ttacttgttt gttttatata accgagcttc ataaggtgct agtatgcctt tagcttcaaa
    12661 ttctccgcta gaggaatttg acaagagtaa tgtccaggac tgatctgctt cttttggaag
    12721 ttcatagtca gcaagtgatt ctgttaagtt taccataata agatattgtt catcttctaa
    12781 atatctttca tagacataaa gttgtggatg ttcatgaaga tattctttat aagaaccgta
    12841 tatcagacct ttattggttt gtctaatgcg gatcatgttc ttatagaatt gcaggaccga
    12901 ctctggttca tattcttgtt gttctacatt gatcgttttg gtatttggat tagtgatcag
    12961 ccaaggttca tgtgtagaaa aaccagcata ttgttccgaa gtccattgca tgggtgtcct
    13021 gctgttgtct ctcgttgttt cacgaatgat atccaatgct tcttctcttg tcttaccttc
    13081 cgcaagcagc cttttataaa gtcttttcga atctactgca tcaacttgat cgatcgattc
    13141 aaagggcata ttcgtcattc cgatttcctg tccttgataa ataaatggcg taccttgcaa
    13201 aaagaaatac atcatagcga ttgcttttgc agacatagcc caaaaatcgg gacgagtatc
    13261 tccaaacact gaaacggcac gaggaacatc atggttttcc atatataaag cgttccatcc
    13321 gataccatcc aatgaagttt gccaagcgct taatgcatgt ttcaatttaa ggacatctaa
    13381 ttgcccttct ttttcttgct gccagatatg gatatgatca aattcgaata tcatatcgaa
    13441 ataaccgtct ttccctaccc attgtggtcc ttcttctgca gtgacaccac ttgcttctcc
    13501 gacagtcatg atatcgtact cttcaaaaac atgtttcaaa tcggtcaaat gttcttcgat
    13561 tcctgagaca ttttgaaagg gtgagaaagg gttctctgtg gcttttactg aatattcatc
    13621 tttttttaca tgagaaatcg catctaaacg aaatccatct atccctttat ccaaccacca
    13681 tcggatcata ttgaatagtt cttctttcaa tttctcgctt tcccaattta agtctggttg
    13741 ttcttttgca aaaacatgca gataatactg tccagtttca tgagaatact cccaagcaga
    13801 accgccgaaa atcgatgtcc aattattcgg ttctttgcca tctaccccgt cgacccaaat
    13861 gtaataatcg cgatacgggc tttctttaga ttttttcgat tcttcaaacc attgatgctg
    13921 atccgaacta tggttgatga caagatccat aatcactttg atccctaatt catgtgcttc
    13981 ttttagcaat aaatcaaaat cttccatcgt tccgaatctc tcttctatat cttgatagtc
    14041 acttacgtca taaccattat cgacaaatgg agattgatag atcggattga tccagataaa
    14101 gccgatacca agatctttca aataattaag tttttcacgg atcccattga tgtctccttg
    14161 accatcgcca ttgctatctt tgaaactagc aggatagatt tgatagccta ctgcttcttt
    14221 ccaccatttt tgttcttgta tcatatcgtt tcactccttt aacggtccct cttgattata
    14281 accgattcta ttttagaagt atccttgtta ccggtaacta gaaacgaaaa gctcaacgaa
    14341 tctgctaagt gcgttcgttg agctttattt tatcatagat agcgggcaat cagttgaatc
    14401 atttgcttgg gaaattcatt caagtcagta atatctacaa aacgactttc accgtagatc
    14461 gacttgattt cttctttgtc ttggccaata gctgcggaga caaataggat tccttgacgt
    14521 tcatattctt tcaaaacaga ctggatatct tcttttgctt ttttgcctgt ataatccggc
    14581 aacgcttttg gctgaccgtc actaatatta agcaacagtt tggttgttgc ggattgtctg
    14641 tttaattttt ctgctgctaa acgaagtgca gcaccatcac gattgttttg ccttggtttc
    14701 atagtgacta atttttcatt taaccattga aatccgtctt caaattcttt ataagaaaac
    14761 aaagaagtct tttctcttga cgaacggtct gctgtatctc cgtaaatcaa caatgggata
    14821 tcgactttct tagaaaattc ggcaatggca atcgctgctt tttttgccgc ttcgatccga
    14881 tcatcccgta tcattgaccc agattcatcg atccgtacag ctaccgctaa agaaggttgc
    14941 tcatgtggcg ggttcttttt gtcaaaattt ctcaaatcac catatgctac tttgcttgca
    15001 ttgaaccgct gaccttcgta ttttcctttt tggtacgtct ctgtttgttc attatccagt
    15061 aactctaaaa tttgccggct aagtgattct actattggca ttacttgcat gtgtagctca
    15121 cgtgcttccg gcgtttttcc ttggaaagct ggtcggtgaa cgatgagacc ttctttttca
    15181 tgaatcgttt ccttcatgat ttgtcgcgct tctttattca gttgcttctt gatttcctta
    15241 tcatgtgctt ccatgtcaga agcagacatt tcttgagaat catctcctgt ttgttgttct
    15301 ttttgatttt ttccttcgat atcgctactg cgttcttttt gcatttgagg aggaatctcg
    15361 tttttttcta ttgtttcttc ttgtttctct tctccctcat ttggttcttc agcagacaac
    15421 gatttctttt catctaagcg cagaatttct tcagcatttt cagtgatgtt tcgttcattt
    15481 gcttcttcgg cgctaatggt tctttctttt gtatgctcag attgaggatg accccatttt
    15541 tcttttctca atatgtcctg aagttccagt agcaatccac tgtcagacaa agcttgttca
    15601 agaattgcca attcatctga atcagtggat aatttataca aaacttttgg ataaatcgac
    15661 tggagtaaat cttgtccttg cttataggta tttgcccaat aaaaataaga acgcattcct
    15721 gctacacctt tgatggcgtt agcttttgca gtttcatcca acaaacggat aatctctgcc
    15781 atctttaaga gaagttcttg tttttggatc atagttttcg ccatcgcacg ttctgccatg
    15841 acttctaagg caggaagatc cattttttcc gcatgttgca tccgatctct taaagattca
    15901 tttaatgggc gatttccttg gtaatttcga tttgtagtga tcacaatgac gcaatctgga
    15961 tggcgacgaa tgattcctgt cggtaaattc aacatcccat ttggttcgag cgctgagtta
    16021 agtgccacaa gaacagaggc atctcggatg acagtcggct cttgaatctc taataaatag
    16081 cctttttcaa tcgctcgaac gatttcagaa gggtaatagc tgtaatggat tgcatctgga
    16141 taatcagctt cgatcctttg aactagctct gctaattttt ctttagcaga aaaaggatca
    16201 atgccgtaat ggttctggat cagtgtcagt acagcttcaa gtgtatctgt ttggtaaata
    16261 gcttctagta attcttggtc ttgttcctta ttagaatcta ctacaggtaa taaggctccg
    16321 aaaacatctg atttgtccat atctgcaaaa caggtcactt ttgtataggg aagttgcaga
    16381 tcagcagata gtgctttggc taattgtgtc ttccccgacc cggcatctcc ttccaaaagt
    16441 acgttactga ttttcatttc aggatcttgc caattccctt tgatttcgga ggcgatccgc
    16501 cattcttctg cacttgtccg atggcttttc ggtttcttcc agatcatttg cttttctagc
    16561 cgtgaaaagg ttcggttttc tgttagtgag aaattctcca tgcttctctt ccccttttga
    16621 tcatctctgt ctattaatat cctagctcat ctaaaagacg ttttaaaata cggaatcgtt
    16681 ccccaatcag ttctccgtct ttttgtagtg tatcgtaata caaaatgatg tcttcatcga
    16741 tctttggatt tttggtatct acttctaccg tcattcggtt cccttgtaat ccaatgatgt
    16801 ctacgactgg attttctgga tcataaaatt tttcataacg ataagcatc
//
LOCUS       DAYLZO010000065        16375 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.066, whole genome shotgun sequence.
ACCESSION   DAYLZO010000065 DAYLZO010000000
VERSION     DAYLZO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.066"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..569)
                     /locus_tag="ACGVJG_001865"
     CDS             complement(<1..569)
                     /locus_tag="ACGVJG_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00374 family protein"
                     /protein_id="HFJ6557799.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFF"
     gene            710..1516
                     /gene="proC"
                     /locus_tag="ACGVJG_001866"
     CDS             710..1516
                     /gene="proC"
                     /locus_tag="ACGVJG_001866"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFJ6557800.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(1595..3151)
                     /gene="rny"
                     /locus_tag="ACGVJG_001867"
     CDS             complement(1595..3151)
                     /gene="rny"
                     /locus_tag="ACGVJG_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFJ6557801.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(3562..4611)
                     /gene="recA"
                     /locus_tag="ACGVJG_001868"
     CDS             complement(3562..4611)
                     /gene="recA"
                     /locus_tag="ACGVJG_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFJ6557802.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(4720..5961)
                     /locus_tag="ACGVJG_001869"
     CDS             complement(4720..5961)
                     /locus_tag="ACGVJG_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFJ6557803.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(6201..6779)
                     /gene="pgsA"
                     /locus_tag="ACGVJG_001870"
     CDS             complement(6201..6779)
                     /gene="pgsA"
                     /locus_tag="ACGVJG_001870"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFJ6557804.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(6988..7911)
                     /locus_tag="ACGVJG_001871"
     CDS             complement(6988..7911)
                     /locus_tag="ACGVJG_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFJ6557805.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(8650..9936)
                     /locus_tag="ACGVJG_001872"
     CDS             complement(8650..9936)
                     /locus_tag="ACGVJG_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFJ6557806.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(9929..11197)
                     /locus_tag="ACGVJG_001873"
     CDS             complement(9929..11197)
                     /locus_tag="ACGVJG_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFJ6557807.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            11449..11910
                     /gene="mscL"
                     /locus_tag="ACGVJG_001874"
     CDS             11449..11910
                     /gene="mscL"
                     /locus_tag="ACGVJG_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFJ6557808.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(11969..12415)
                     /locus_tag="ACGVJG_001875"
     CDS             complement(11969..12415)
                     /locus_tag="ACGVJG_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6557809.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(12508..12753)
                     /locus_tag="ACGVJG_001876"
     CDS             complement(12508..12753)
                     /locus_tag="ACGVJG_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFJ6557810.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(12868..13446)
                     /locus_tag="ACGVJG_001877"
     CDS             complement(12868..13446)
                     /locus_tag="ACGVJG_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFJ6557811.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(13523..14053)
                     /locus_tag="ACGVJG_001878"
     CDS             complement(13523..14053)
                     /locus_tag="ACGVJG_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFJ6557812.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(14057..14917)
                     /gene="rlmB"
                     /locus_tag="ACGVJG_001879"
     CDS             complement(14057..14917)
                     /gene="rlmB"
                     /locus_tag="ACGVJG_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFJ6557813.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(14910..15305)
                     /locus_tag="ACGVJG_001880"
     CDS             complement(14910..15305)
                     /locus_tag="ACGVJG_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFJ6557814.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(15302..>16375)
                     /gene="cysS"
                     /locus_tag="ACGVJG_001881"
     CDS             complement(15302..>16375)
                     /gene="cysS"
                     /locus_tag="ACGVJG_001881"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFJ6557815.1"
                     /translation="VMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQ
                     SIDELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMA
                     TKHLGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSK
                     SLGNFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLK
                     FRQETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQP
                     KVSAAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIR
                     DLLKEKGILLEDTPQGTRWRREA"
BASE COUNT         4472 a         3560 c         2893 g         5450 t
ORIGIN      
        1 aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa gccagatcca atagcttcga
       61 aaaatacttt ctgactgaat aaaaaagaca tcaataaatg taagaaacga ccatacagac
      121 aaaccagtca gcataaatag tgcgactttt gatacagtgg ctaaaaccat ttttctattt
      181 gtctgctttc cataaaaact agctcgcagg cttgctccta ccacaccacc aaaaccggcg
      241 agattgttga tcgtgtttgt tacccacgca gcaagaagaa actcttttct ttccattttt
      301 ggttttcctt gtttttctaa gactctgatc gtcacccaat catacaacaa catcggagtg
      361 acacccagca gtccagccag aatcatgagc aggatcgtca tgcggctttg ttgtcccatc
      421 ctttgccaaa tatcttgcca actcatgcca ttagcaatat ttgcgacttg atttgccaca
      481 aagactaaca cggagccgaa aaaaatcagt tttaaaatca gactatgttc ctttatccat
      541 tgcagcatca agtatcgttt atttttcaca ttttcccatt cctaacttgt atacttttgt
      601 gtcatacccc aatgttatct tatttatttt aatttttcca ctttagacct gttctttgct
      661 tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga gaggataaca tgaaagtagg
      721 atttatcggc acagggaata tggcacaagc tattattgat ggtttattaa aaaaaggaac
      781 agtctcgaaa gaagaggtgt tcgtttccag tggacatttt gaaaatgcac aaaaatttgc
      841 tgctgaaact ggcgtccatg catgcaaaag taatcaggaa gttgcatcac aatcagatat
      901 cgttctttta gccgtgaaac caaaaatcat tgaaaaagta ttggaagaac taagagaata
      961 tagccagaaa gtcttattca tttctattgc ttcaggaaaa tcattaatag atttagaaac
     1021 agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg ccgaatgtca atgtcagcgt
     1081 aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc acggaggaac agcttcagca
     1141 agctacgatg atttttgaag caatcggcag cgtctatcct ttagcagaat cagatttcag
     1201 tacattcatt ggccttgctg gcagctcacc cgcattcgtt tacatgctga tcgatgcgat
     1261 gggacggact ggtgtactga atggtttgtc caaacagaca gccactgaga ttgctgcaaa
     1321 agcaattatg ggttcttgtc aaaaattcct ttcaagtgat aaaaatccat gggaacttgt
     1381 agaccaagtt tcttctcctg gcggtacaac tgttgcaggt gtagttgcct tagaagaagc
     1441 aggattcatc ccagcaatca tcaaaggaat caatacgacg atcgagaaag atcttgagat
     1501 gttgaataaa aaataatgat agcaatgttt tttcgtgcct tacaaaaaaa gaagagctgt
     1561 ggcttttgcc gcggctcttc ttttgtattt tttattattt agcataatca gttgcacgag
     1621 tttcccgaat aacgattact ttgatgtgtc caggataatc cagttcttct tcgattcgtt
     1681 tacgaatatc acgtactaaa cgaacagcgt ctaaatccga gatttcttct ggcttgacca
     1741 ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga ttctacaccg gcgaagctgt
     1801 tcgaaatatt ttccagactt cttaaacgac ggatgtagtt ttctaatgat tcacttcgcg
     1861 caccaggtct tgctgctgag agcgcatctg ctgcagctac aaggacagag ataacagaag
     1921 tagcttccac atctccatga tgagaagcga tcgcgttgat tactactggg ttttctttgt
     1981 acttggcagc taattccgca ccgatctcaa cgtgagatcc ttcaatctca tggtcaagtg
     2041 ctttcccaat atcatggagc aacccggcac gttttgctaa ttggatatct tcgccaagct
     2101 ctgctgctaa gataccagct aattttgcaa cctcgaccga atgcttcaag acgttttgtc
     2161 cataactagt tctgaatcgc agacgtccga tgatcttgat tagatctgga tgaagtgtat
     2221 gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg aatacgttca tccatttctt
     2281 tacgagattt ctcgaccatt tcttcgatac gcgctggatg gattcgtcca tcttgtatca
     2341 gtttttccaa ggtcatccgt gcgatttctc ttcggattgg atcgaatccg gaaagaacaa
     2401 ctgcttccgg tgtatcatcg atgattaaat cgataccagt cagcgtctca agcgttcgaa
     2461 tattacgacc ttctcgtcca atgatacgac ctttcatttc atcattcgga agggtaacga
     2521 ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg ttgaatcgct aatgataata
     2581 agtttttcgc tttgcgatct gcttcttctt tggcacgctg ttcagattct tttaccatta
     2641 gagttaactc atgattcagc tcttcttcag tcgatttcat gatgacatct cgtgcgtcct
     2701 cttttgataa accagcaatt ttttctagtt ctttttgttg atcttcaatc attttttcaa
     2761 cgtctttttc tcgctcttca attaattgct gttttgaact aagtgcttct tctttacttt
     2821 caagtgagtt ctcgcgtttt tctaacgagt catctttacg attcagcgtt tgttcgcgtt
     2881 gaatcaaacg attctcttga gattttaatt ctaacttact ttctctcagc tcactttcaa
     2941 cttcagaacg gtatttctga ttttcttcct tagcttctaa caatgcttct ttttttagag
     3001 tttctgcttc tttttttgca ctatcgatga ttccagcagc tgatgattgt gcaccagcta
     3061 tttctttctc gtgacgtgac tttgcgacca taaaccctaa accaagaccg acaattaaac
     3121 cgatgatagc gaggagaatg ttgaatacca taaaatccac ctccatacta tctttttcat
     3181 ttttatcatc tatacagctt ctatgataaa ttattctaaa ctcgtcaata tgcatcttcg
     3241 catatatgtg tacaaaaaac atgcacaact ttattctaat gtttgtaccc cgtagtgtca
     3301 actttaaata aacaaagaaa cggctttcta cttattcatt aaacgatggt ttttaaaata
     3361 aatgacacta gtttcatata ggtaaacctt taaattgaga aacttttcta ttaaattatt
     3421 ctttgtagat gaacagatat ttttatttta tgtacccact cgtataatta aataactcac
     3481 gatttcttta ttcaataaaa aaacggcagg tcaaggagag tacctactct tcagaccagc
     3541 cgtttttgtt tatcataatt tttattcttc tactaaaggc aattcttctt gtcctttttc
     3601 atcttctggg acagcgactt cttcaccaat accataagct gctctaacta gagcagaaac
     3661 ttctgccatc atttcaggat gattagccat atagatttta gcgttttccc gtccttgacc
     3721 aatccgatct tctttataag aataccaagc accgctttta tcaacaatgt ctttttctac
     3781 tgccatgtct agaagttctc cttcttgtga gatccctaaa ccatacatga catctacttc
     3841 agcgattttg aaaggaggtg ctactttgtt ttttacgact ttgatctttg tacggttacc
     3901 aacaatatct gtaccttgtt tcaattgttc tgcacgacgt acttccaaac ggatcgttgc
     3961 gtagaatttc aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca tcacgcctac
     4021 tttttcacgg atttggttga tgaaaatagc gattgttttt gttttgttga tcgaaccaga
     4081 gagtttacgc aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg agtcacccat
     4141 ttcgccgtcg atttctgctc gtggaactaa agcagcaact gaatcgacta ctacgatatc
     4201 tacagcccca cttgatacta aagcatcagc gatctctaga ccttgttctc ctgtgtcagg
     4261 ctgtgaaaga agtagttcat cgatattcac acctaatttt tgtgcatatt gcggatctaa
     4321 cgcatgctca gcatcaatga aagcggccgt tccgccattt ttttgtactt ctgcaatagc
     4381 gtgtagtgca actgttgttt tacctgaact ttcaggtcca tatacttcga tgatacgtcc
     4441 acgggggtaa ccgccgacac ctaaagcgac atctaacgca agagagccac ttggaattgt
     4501 agagatttgt tggtcgattt tttcgcccaa tttcataata gagcctttac catagctctt
     4561 ttcaattttt ttcagtgcag catctaaggc tgctttacga tcatctgcca atacgataat
     4621 cctccttata tttacaaatc agagcaacat ttcattatcc ttatcatatc ttttttagtg
     4681 aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat cattttctta aaagctgttt
     4741 tctcaaaaga tcaaatcctt gcataaccgc attatatctg atagcagcac gatctctggc
     4801 aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc atactgttcc
     4861 tgcagattga ccttctaacg tgtccggtcc agccactccg gtgaaagcta cactgtaatc
     4921 tgttcttgct atctcacgtg cttgaatggc catctgctcc acacattctt tgctgactgt
     4981 tccatattct gctaatagct ccggatcaat ctccagaaaa ccagcttttg tttggagcga
     5041 gtaggtaacg aatccgcctg gaaatacttc agatacacca gcaatgtcgc ctaatgcggc
     5101 ttggaaggcg ccggctgtca aactttccgc tgctgttaca gtctgcttgt tttctttcag
     5161 tagttccact acgacttttg ctagcgaatt atcatcgccg tatccataaa aatattcccc
     5221 tactcgttct tggatttttt cttctaatgc taaaagagct tgattccctg cctgaacatc
     5281 atttgttttc actgtcagtc gtaatgtcac ttcatttggt ttggcatatg gtgcgatcgt
     5341 tggattgatc tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg attcaccaat
     5401 cccataaaag cgcaaaactt tagaaattag tttttcttca gaaggaaaac gttcctctag
     5461 taaaggacgt acttgttcca caaacattgg tttcaactca ttaggcggac cgggaagaag
     5521 gatatacgca tgtgtatctg tttgataaaa agtccctaaa gcaagccctg tacgatttgg
     5581 tagaggaacc ccgccttcga taatctggga ctgttgcaga ttgtttttgg tcatttttcg
     5641 atgggttgtt tcaaaatatg ccaataattt tttgtatcct tccgtatttt gtatgagaga
     5701 cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg gtcctaatcc
     5761 gccacataag agtatgagtt cacttcttgt ttcagctaga gcaatcaact cttctaaacg
     5821 ctgctgatta tctcctacaa ctgtttgata ataaacttcg atacctagat cagctagctg
     5881 ttctgacaag aaagtggcat tcgtattcac gacttgtcct agtaaaagtt ctgttccgac
     5941 agctattatt tctgctttca ttacttcccg ctcccttata tagtatacgc tgtattttta
     6001 tattttttta tttaaaaaga gggcaaggag ctgtgacaaa aatcttgtca cagctccttg
     6061 ttccaaataa acagtgttct cctgccaaaa cctcggtcat aagtcacaat atacataaaa
     6121 catgaaaagc tgttttttga aattatccct tgtgactcag agttagacgg caggctcaca
     6181 acctctccac taaacgatac ttacattgat cctttgaaca caccgctgtt tttgatgaag
     6241 taatccacac ctgaatagat agtaaagatc agacaagcat aaagcatgat ttgatctact
     6301 ggaataccga tcaggctgaa tgggatatta ttgataaaca gcaagataat agcaaccatt
     6361 tgtgtagccg tcttgacttt tcccggccaa gctgcagcca tgacttctcc atgttcgaca
     6421 agtagtaaac gcaagcctgt gacggctaat tcacgacaga caatgatcgc tactacccaa
     6481 ccaggggcct ttccttggtc aaccaacatg ataaaagcag tcataactag cattttatct
     6541 gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc ctcgcgcacg ggcaattttt
     6601 ccatctaacc agtctgtgat actagctaaa gcaaaaataa ctgcacctac tagctgtgtg
     6661 acagccagtg tggtgtctcc tacgttaaat tgtccccaat ctaaaggtaa acctacaacc
     6721 aaaatgaaaa tcgggatcat acaaattcta agtacagtta atttatttgg taaattcaca
     6781 ctatcgactc ctttcttccc tattctacgt gactatcagc gaagtttcca ccgatattat
     6841 ttacttttcc aaatgaattt attcaaagga gtatttttat tgaaattgat ctttttgaat
     6901 taacgaagaa ggaccgcgac acagatttgt cacgatcctt cttttttata cctacaagta
     6961 aacagatgcg taaactaatt tatacgatca ttctgcgtat tgaagagtaa ggttcacatt
     7021 ttttggactc tgattgtttt cccctggatt aactggtacg gattctccat tcgctttgat
     7081 cgttgcattg cttgctgcac caatcgtgat ggtcgcattt gttgctgttt ccggtaacac
     7141 ggtactctgt gtttcatttg ctgccagtgt cccttgataa acataggcat tgtctactag
     7201 cacacctaac catacacgat tgacagcagt aaattcaagg gttacaggct tggtagcgtc
     7261 tgtgatattt actgcgatgg cactgccaga atcttctcct gtagctattg ccattttctt
     7321 ttcttcactt gattttgttg ttgattctac tgtagaagac tctttggtct gcgtgctttg
     7381 ctctgaggaa gaggactcct cgactgatcc ttctacttta agcgaagaag ctgtgtctcc
     7441 gattattgga tcagcttgac gatcttgcca agtcatatag cccacgacaa cgacaatcgc
     7501 caaagcaacc aatcctaata aaatgacagg aagacttgtc caaaagcgac ttggatgcga
     7561 ttcttcctga tgcattgctt ttctagaagt acttactgtt tcaggctgcg gacgttcagg
     7621 gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg actaaatggt ctccatcttc
     7681 tcctacagct tcagcgtatt gccgaataaa tgcccgcacg taaaatttac caggcaattg
     7741 gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt tgaatttttg taatctgctg
     7801 taattcatct aaagatattt ttttattcag gcgtgcttgc cgtaattttt ctccgatggt
     7861 ttcaaaagcc actcgttcat cttctccagt ctgtttgatt ctccgaagca tctgctgttg
     7921 caacgaaaga cgttatgttt gggtgctgtg acaaaagtca tgtcacagct ccttttttcc
     7981 gaataaacat tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aataaatata
     8041 agaagctgtt ttttgaactt gttccttgtg actcggagtt aaacggcagg ctcacgacct
     8101 ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta gctcaaaaac cggcaaaata
     8161 taaggagata tttttccgta tgtttctcta atactcaggt caaaacgctg atttcacaac
     8221 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa
     8281 aacattaaga agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc
     8341 acaacctctt ggtacacggt gttccagaag tagcttcttc ggcaataagc cgaaatatca
     8401 caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct
     8461 gtcacaacct ctttgattac ttcgtttgat tttattttaa tataagttga ttaaaagaga
     8521 gcatgatttg ttttattttc tcactaattt ttccaacaca actaagaata ccataaaagc
     8581 tgattttgga ggaggttcct actacaagtc taaaacgaaa tttaagactt ttcctcataa
     8641 aatactggtt tattttggtc tcatgtaaaa ctcgctgaag gcctcttcac ttataaatgc
     8701 ttccccagct gccagtacgt ctttcatttg gatcgaatcg atgatttcag gaagatcaaa
     8761 taaagttcga tcgccaaata aactttgcgt aaattgattg gcaatatatt cgatcgaatt
     8821 taatgattgg aaatattgtc caagcatttt cttcttcagc aaatcgagat ttgtttctga
     8881 cacttccgga tcatcagcga atccaagaat gatttctttg actttttctg ctgctttctc
     8941 tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat tcacgatcca agctgaattc
     9001 aaaaccaaag ctgtcatcga taattccttg attgtacatc gctagatagt ttcgagaagt
     9061 attgcctaaa atcatttgga acaacagatt gattgctgtt ttatagcgta acagttctgt
     9121 tccttcttgc ggcaatgtgt ctaatccttt aataccaagt acaaatttat ctcgtgtaat
     9181 ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg ttttcaggga aataacgtac
     9241 aatttcttgc tttggaggga aattttttgc ttcttggttt tcccgaataa gtttcatcaa
     9301 tttctcaggc tccatcttac caactacaaa taaaaccata ttgcttggct gataaaacgt
     9361 ccgatagcac gtatacaagt cttgagctgt gattttgtcg atactttcaa ccgtgcccgc
     9421 aatatcgata tgcaatgggt gtgtaggata caagttattt agtatgccaa aaaacatccg
     9481 ccagttaggg tcgtcttcat acatttggat ttcctgaccg atgattcctt tttccttgtt
     9541 tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg atcagagtcg tcagattctt
     9601 ttctacctga tccgtcgtag aaaacagata gctggttttc gtaaaacttg tgaaagcatt
     9661 ggcggaagct ccttgtttcc caaataattg aaagacatca ccatcttctt tttcaaacaa
     9721 cttatgttct aaaaaatggg caattccatc tggaactttc accttttctt tttcaccata
     9781 gggaataaat tcgttatcga tcgaaccata attcgtactg aacaaaccat atgtcttgtg
     9841 atagtcattt ttaggcaaca aatacacggt caaaccgttt ggcagtactt catgataaag
     9901 tgtttcattg atttgctcat attctgtttt attcatttgc atttcctcct gctaaaaaga
     9961 agattgcctg caattcgatt tgctcagcta cttgttgtat ctctttgatc gttacgctct
    10021 ccatacgttt caaccattct tcatcagata attttgtttc cggcagccag ctgtccaaaa
    10081 aagaagcttc gattgccgct tgcggagagt ctagagaaag cagatattgg tttctcagca
    10141 ttgcttttgt ctgagacagt tccaactctg ttatttcacc atttcgcaaa ctttctaatt
    10201 gctcgtgaat cagatgaagt acttgatttc ggttcttttc atcgataccg gtttggacac
    10261 tcatgaatcc tcggaacgta tccacgctac tagaggcata ataagctaga ctttctttct
    10321 ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc aaataaaccg ttaaagacca
    10381 ttagcgcaaa acgttctggt tcatcgtaat acacatttgt ctgataagct aggttcagct
    10441 ttgcttgaac gattggctca cgtacttgtc gttccttcac gatattcact tgaggttggg
    10501 tataaaacag atctggataa actgtttccc gcgactcaaa tggtaattgc ccaactgctt
    10561 cagctgcttt gtccggatca acatcgccga caacaaaaat gtcgatttga tcatttgcca
    10621 tcatttcttg atagtaggac gctagcgaac tagcagtgat cttgtccaaa tcttcgacta
    10681 cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa ataaagttct tgcaaggcat
    10741 aggaagcaaa agtttgttta tcttccttca aactttccaa atatagacgc agattgttct
    10801 tttccaagtc aaacgtcaac tgatcaaact gatgattttt aatatttgga taaaataaaa
    10861 cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg atcattgaca tattcaccat
    10921 tcacaaaact gatccctgcg tttagccaat gtaaatttcc tttttttcta actgataatc
    10981 caaagcttgc gccataaagc tctgcaagtt gcgcacttaa ctttgtctga tccgaataat
    11041 tcaaactatt tgtttccaac atgctgctga gaagtgaacg tttcgtgatg acttcttgtt
    11101 gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt gtattgtttt gttggtaaga
    11161 catgaagatt cacgccttct gccaattgat acaccatatt tatatgctcc ttttcacagt
    11221 tgattgcccg cttactcttt taatgggtca acctgccttt tttacttatt cctctgcttg
    11281 aaatttataa gttcaggcaa ttcgccgttt tttctaaatg atgttacact ttatagtata
    11341 gcatagatgt tttttatcaa aagaaacaga cggctaaaag cgtatattgt ttttttattt
    11401 tttatttttc gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa
    11461 ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt
    11521 agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt
    11581 tggtttgtcg caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac
    11641 tggtcgccga caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca
    11701 tttctgatca ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat
    11761 ttacgctcca aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt
    11821 agtgacatac gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag
    11881 caaacaaatc tttccgatac ttctaaataa taaagaatct aaaaaaaaga aagccgattt
    11941 tcagtgtcgg ctttcttttt ttagattctt aacttgctac ttgttccttt ttcatgtcga
    12001 tatgtggaat atcatcttcc aaatacacat ctgagattgc cttgaatcca aacgactcat
    12061 aaaaagcttg taggtattct tgtgcgccga tcacgattgg tttttctgga aaatttttct
    12121 ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa accatcttta cggttgtcct
    12181 gatgaacaag gacacgtcca aaatggatac tctccgcttc ttcatatact cgcgtgtagg
    12241 caaggatctt cccatcagta tcttgaaaaa acgtatgcca agcttctcca tcaatgtcat
    12301 caacttcttg ataaggacac tgttgttcca caacaaaaac tgcgattcgt tctttgacga
    12361 tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt tacctgatat tccactaagc
    12421 aaacgccccc tgctttttta ttcttagcat accgattcgc acacaaaaaa gcaaacgaac
    12481 cttacgaatc tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac
    12541 tgtccgagta agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg
    12601 atccaaatca acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt
    12661 ttgacgcttt cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa
    12721 atctttcttg attgaagcta aagttgttgg catacagttc acctctacta tcaactatac
    12781 cacatatttt taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa
    12841 taagaagctc attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt
    12901 tattttgagt tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca
    12961 ttcagccatg tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat
    13021 ttttgaaaac tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaattgc
    13081 taaaggttct tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg
    13141 gtcgattgag acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag
    13201 agaatagatt tgattgctta taacgctttt ataataactt ccaaagctaa cacctttgct
    13261 tttttcgtaa gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc
    13321 agaaacataa tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt
    13381 ttgatacagg gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc
    13441 agacataaaa taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca
    13501 tcgaactgat tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg
    13561 acaatttttt taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca
    13621 tatctttatt ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat
    13681 aagcagaagt tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg
    13741 tcgccactgt cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac
    13801 tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt
    13861 gttggatgcc cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt
    13921 tcgcataatt tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct
    13981 ttttaagctg gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca
    14041 gctgtttttt catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac
    14101 cagcagcaac actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt
    14161 catccatttc ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag
    14221 caattgatcc tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata
    14281 cccaaaatcc tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa
    14341 cagggacatg ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg
    14401 ctcggtgttt tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg
    14461 acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt
    14521 tctcttttgt tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa
    14581 gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc
    14641 atttgactgg cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc
    14701 gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg
    14761 catggtgccc aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact
    14821 gatttcgtcc agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac
    14881 gtttcgattg atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata
    14941 caccagcgga tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg
    15001 agcgcttcga accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta
    15061 tggcttttag catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt
    15121 tcttccagca tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc
    15181 gcacgatgca gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag
    15241 atttcgtaaa tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct
    15301 ctcatgcttc tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt
    15361 ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat
    15421 ttcgttcttc gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa
    15481 taccgaagat cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag
    15541 aaactttcgg ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga
    15601 tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt
    15661 ctaacaaagc gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga
    15721 atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg
    15781 tcgtttcact gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga
    15841 cttgtggatc aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac
    15901 tcattttttc atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga
    15961 attttttccc tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta
    16021 aatcttgccc gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg
    16081 aacattcaat atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc
    16141 caggttttgc ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt
    16201 cccctgtacg ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc
    16261 cataattttc aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc
    16321 ctttttcaac caacacttga ataaatgcta aaatatctgg catatgatcc atgac
//
LOCUS       DAYLZO010000066        16274 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.067, whole genome shotgun sequence.
ACCESSION   DAYLZO010000066 DAYLZO010000000
VERSION     DAYLZO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.067"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(855..1385)
                     /locus_tag="ACGVJG_001882"
     CDS             complement(855..1385)
                     /locus_tag="ACGVJG_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFJ6557816.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1382..1999)
                     /locus_tag="ACGVJG_001883"
     CDS             complement(1382..1999)
                     /locus_tag="ACGVJG_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFJ6557817.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2581..2802)
                     /locus_tag="ACGVJG_001884"
     CDS             complement(2581..2802)
                     /locus_tag="ACGVJG_001884"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFJ6557818.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(2980..4512)
                     /locus_tag="ACGVJG_001885"
     CDS             complement(2980..4512)
                     /locus_tag="ACGVJG_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFJ6557819.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4648..5733)
                     /locus_tag="ACGVJG_001886"
     CDS             complement(4648..5733)
                     /locus_tag="ACGVJG_001886"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFJ6557820.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5748..6467)
                     /locus_tag="ACGVJG_001887"
     CDS             complement(5748..6467)
                     /locus_tag="ACGVJG_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFJ6557821.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6464..7774)
                     /locus_tag="ACGVJG_001888"
     CDS             complement(6464..7774)
                     /locus_tag="ACGVJG_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFJ6557822.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(7801..8391)
                     /locus_tag="ACGVJG_001889"
     CDS             complement(7801..8391)
                     /locus_tag="ACGVJG_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFJ6557823.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8709..9770)
                     /locus_tag="ACGVJG_001890"
     CDS             complement(8709..9770)
                     /locus_tag="ACGVJG_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFJ6557824.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10338..11405)
                     /locus_tag="ACGVJG_001891"
     CDS             complement(10338..11405)
                     /locus_tag="ACGVJG_001891"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFJ6557825.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11425..11904)
                     /gene="pssE"
                     /locus_tag="ACGVJG_001892"
     CDS             complement(11425..11904)
                     /gene="pssE"
                     /locus_tag="ACGVJG_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFJ6557826.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(11921..12382)
                     /gene="pssD"
                     /locus_tag="ACGVJG_001893"
     CDS             complement(11921..12382)
                     /gene="pssD"
                     /locus_tag="ACGVJG_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFJ6557827.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12396..13646)
                     /locus_tag="ACGVJG_001894"
     CDS             complement(12396..13646)
                     /locus_tag="ACGVJG_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFJ6557828.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14024..15475)
                     /locus_tag="ACGVJG_001895"
     CDS             complement(14024..15475)
                     /locus_tag="ACGVJG_001895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFJ6557829.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15798..>16274)
                     /locus_tag="ACGVJG_001896"
     CDS             complement(15798..>16274)
                     /locus_tag="ACGVJG_001896"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFJ6557830.1"
                     /translation="LFTDLDDTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPER
                     NVHFREDPNHLLPFLDMGCLAQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHG
                     VSKRTFYLKECYEQIAKDFGKEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
BASE COUNT         5406 a         3220 c         1881 g         5767 t
ORIGIN      
        1 aatttaaaat taacattgat tctcctatga aaatatcttt tacctttctc ctgtgaaaag
       61 gaaatttcct tttttatagt gttattgata taatcagaat attcatcaaa ctctgtatct
      121 ggagttaaaa atctacagta acatctataa gctttgctag taatcttctt gggttctctc
      181 tccattgtca agcaacatca aaacacctac ttactattcg attcttttct gccaaaaacg
      241 agtcagcatc actcacaact tatgtgttgc tgactccgac tagtcctttg ttcaatatca
      301 acttattaaa cagatataat atttttcttt atttcgtata ctccaagact tccatctttc
      361 tttcatctta tctctgaaaa aaacacagca aagtgccctt aataaatata cattagaatt
      421 tgcaaatttt ttcatcttaa gatatatcct attcatattc tttacaatcc taatgggtga
      481 ttacaaatct atttagttca agttctacat tataaacacc tttcttgata gtatcatctg
      541 ctgcacaagc gatgtcgatc cacttattag gggatttacg tgctcaaaag tggctattcc
      601 ttttgggaag tccatccata ataatggtcc atggattttt tagggtcttg tcgttcaaat
      661 ttctcaaaaa ctttattata cttccatagt tttataatta tttggtttca ctttggctcg
      721 ttcaaacacc aacttgtatt tcttttcaac gttcgaactg gagccccccc tataatctca
      781 ttattatttt catgactttt tactaccgtg gaattacaag caacaacaat attatctgct
      841 aaatgaacac ctttctatat cacgcttttt gctccaatcc aacaatgttt acctataact
      901 attttattat ttttattcaa aatcctatga gtattcaatt caaaaatcgt atgtgaatcg
      961 gtatccataa ataatgtatc ccacgatact gtacagccat ctttgaattg aatttcctta
     1021 aagcaaatta tactcatttt tcctgtcgaa ttaaactttg acccaatatc taaaactgca
     1081 tttttcctta tgtcaatatg agccccttgt gcgataaacg ctggcccttg tattcttagt
     1141 attccttcaa tacttataat cgtttcttca tttcccgcag tatttaatac tggaaagccc
     1201 aaataaacta tttttccaga ttgtaatact atctcaattt tccctctcga tttgcgtgta
     1261 atccgtactt tacgatgcac caaaataggt aatctccacg catctgaaat atgaagaaat
     1321 ttataattga agtatatcgt tttaggtaaa tttaatatat tctttattat caatccgagt
     1381 ctcatctttc accatctctt tttgtaatca aaattagttt ataaaggatt acacccaata
     1441 taccgcccaa cgtattatat acaaaatccg aaatttgaaa cgttcctagc ctcaaaaaaa
     1501 gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat ttttcctcca tatactattg
     1561 aggaccatat tttgggtttc tttatatttt catatttaat agacattacc atataaacat
     1621 atggaataaa aagaataata ttttctaaac acgctgtaga taactctttt gttccagttg
     1681 ttgtattata cttataaatc caccagtctc ccataacatc agataaagga tttaaccaaa
     1741 ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt atagaatatc aaagcacaaa
     1801 cttttctata ttttctatcc ttctttaaat gaagataaag ttccattagt atacgtttat
     1861 agccctcacc tgcagcaatg ccatctgttg catacataaa atagaccagt cctaaaattg
     1921 tcaataaaac agcaaaccca aagggttcat ataatttata taatatatct gtaataattt
     1981 ctgcaatcac tattatcata attatttctc caatatcaag tatacactta tcgcttccaa
     2041 ttacacatga tcatattaga gtgtattatc acattctcta atattcaatt tatttttcct
     2101 aaaatattcg aaataatcgt cttgataacg agtaagtatt tttatacgat tgataacata
     2161 gtaacaatat ttttaattat agtaaaaaat catctgcctt tttcatccac ctaatcttat
     2221 ttttcatttt attcaacatc cttaaataaa cttccgttcc taagctctat aatatcttct
     2281 taaaaaataa tattatggtg tcgttttcaa actcgtaatc ttttacattg gaaaaataca
     2341 tacttttctc aatgtagcaa ctcctatctc acagtattca aaaattcttc aatattatga
     2401 aatacttgtg catgttcttt aaataccttg tcagtctgtt tataatcccc tactaaattt
     2461 tgcattgctc ccaactcttc gacaaattgt catctccatc tgtaaacagc cctaaaaact
     2521 catataatgc tttttcatca atatttttcc catattattc tcggtatagt ttcacaagtt
     2581 ttacggattc ttcatattgt ttatcttttt ataatattca tctagggaca taattacttt
     2641 tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc ttgtttacta aactatttgc
     2701 acctataact acattatttc ctatatgtac accctttagt attctagact tcattcctat
     2761 aaaaacatta ttacctattt tgatcctctc cgatgatccc aaagcttctc catactactt
     2821 ttaaaaccga tcaatcataa acatgagtca aaattgtaac tccttcatat catctccaat
     2881 ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt tcgcctattc ggcagcctaa
     2941 aaattgtagt tactttatat atcattctga tgtctcttct catcttttta ccaataccga
     3001 cgtaagaact ttcaaaatat acttaaactt cttattacgg atattaaaca gaagaataac
     3061 taaaatcgat atgacaaccg atataactaa atttatcact aaacttatca ctttgaaatt
     3121 tccgatatta acatacgaac aagcaaaata acttaaataa gaagcaacaa aagctattac
     3181 acacaaactt atgagcttcc aaacatattt acctgccgat tcaaatttaa atatattttg
     3241 gaaaacatta tataataacc agggtaaatc aaccaacaat actgaaacaa tggttgccgc
     3301 caaaactcca tataatccga accaattaac gaggatgata tttaatacaa tgtttatcat
     3361 tgctgtaatt aatggtctaa acctgtctgc gtaccatatt ccagcagcat ctttgtataa
     3421 cccaattaac tgatcaattt catataagaa aaaatgaact atcataaata taacaaatga
     3481 aatactcata agattatcac ttcccaccca gataatcatg aacggctgaa ataatgtaat
     3541 aaagcatgca caactaattg ccagaaacca acctataatt gttgtaatat cacaaaaatc
     3601 tatataattt ttttcttgac tttctacaat aagactgtta cctatacctg ctatacatga
     3661 ttcataaatt attgcaaata tactcattat ggcagaaata ataaagtaat aattctgata
     3721 aatcgcaagt actgaaagac ctaagaaaga tgaaataata attgaatctg aagatcgtaa
     3781 tattgtaccg cctaccttgt ttgtaactaa tccttttact ttttttataa tatcgtcaat
     3841 agtttctttc tctaactcac caacaggagt ataatcagga tacatctttt gagaaacaac
     3901 agcacacatt aagttaagaa ctatttgtcc caaaatagaa gccaataaat acaaatagta
     3961 attttttaag aaaatcagaa catatgcctg cattacaaat tggaatgtaa aagtaattag
     4021 catagacacg ttaattacat catttctttg atgtgcatat attagactct ttctatatga
     4081 aaataaccaa tacgacaaaa cagtatttat caaatacatt aaatacaaaa ctgtcatatt
     4141 taaatttcca ggaatcgaac ctttaactaa aaatctcaaa aaaggaatgc ataacaatcc
     4201 taatgtaaac actatccatc caataattga aaagtacttt ttatatgcgt tcagtaaagc
     4261 acaaatctct tttctattgt cttctgctat aggtttatac atacttacag ttagtgcagc
     4321 acctattccc aattccgcta aatttaacat ttgaaatata gatgtgaaca ggttgttcaa
     4381 tccagcatac tccattccca tctgataaat cataagtgta cgcataaaaa agggaatgat
     4441 aatctgatat aacttaaaaa ttgttccagt aattatattc cttttagcat tttgtttacg
     4501 attaattatc atatcatatg gctccaaact ttctattttt tattgttacc taatactata
     4561 taatttctaa aatcttgaaa tgtaataggt ttcttctagc tctttgatta attacgctaa
     4621 ttttcatgtt tggacaacca caaaaaacta tacattttca atcaaagcaa cccattgttt
     4681 tgtaatttct ttcaaattaa atttttgtac attattatat gcgttcagtg acattctcat
     4741 tgctaaatca gtattcttag taaacattat catgctctta gctaattggt ctatattctt
     4801 atcttcaacc aacatcccat taaaattatg atgtaccaaa tctctcggac cagttttaca
     4861 atcataagaa ataactggta atccataaga ctgtgcttct aatataacca taggaaatcc
     4921 ttcataaaca gaactaagca cataacaaga tgcagattta taatattcct ccacatggtc
     4981 tgaatgccct gctatagtta ctctttgcag atttaaatct tttatctgct ggcgaagatc
     5041 tttttctaat tcaccttttc ctacaatgat aagatcccaa tcatctactt ttttttctac
     5101 aattttccaa gcacttatca tgtcagcaaa atttttctct tccactaatc gccctactgc
     5161 aagaaatctc tttttatcat aatgatattc gtactcgata ttagatatac acggatttgg
     5221 catagttata acaaaatctt ctgataaact atattcatca atatgttttt gacgatcttc
     5281 ttctgtgaga accacaagcc tctttgcata tttttttatc aattttttag ccttgaatct
     5341 cttcttttct ttcagcattt ccattgtcca gtaattaaaa tgttcccaag aaatcaattt
     5401 gcactttgtt cctttaattg cccttgtagt atacaaagac aacaaagtat caatatcaat
     5461 tactatatca aattcatttt tgcatatata cttgtgcaat ttccaaaccg gatataaata
     5521 agttctatat aattttccct ccgttttagg atttaacata taatcaacct taatcgtctt
     5581 atctaaccca aaataaggct ctccactatt ccaaaagctt aaaatagtga catcataacc
     5641 taaatcagac aagctattgg caataatact ccctactctt tcagttcccc cagaacgaga
     5701 aattacacca ctaatgaaac aaattttctt caaagtactt ttctccttta taagtgtaaa
     5761 aattttttca gtgcaagtaa cctgttaaat tttttttgtc caattgttga ttttaatcta
     5821 taaacaaatt tttgtttagg tgttttccaa gatgcattat attgatgaat gcttattgta
     5881 ttactagttt tataaatttt tccagtatca taatccttgg cagcaaaata atccgtagga
     5941 tagatatgca ggccatttat tatttgttcg ctgttatcgc gaaccaatcc aatagattca
     6001 agataatctg taatataata cggactcggt ttcaagttca atgtaccatc attttgataa
     6061 aaacttaatg aatcgtaaat atcccttaaa cctttaataa cagaatgtcc ttcttctgca
     6121 ccaaaaccaa taccgccatt aacccagatt tccccttcaa atcccatata ggcatgattt
     6181 tcacgaagta tatctaacga cttaattagc tcaacatctg tatctaaata aaatccacca
     6241 tgattataaa taatatcaaa tcttgcataa tctggaacaa aaccccattt acgttcttta
     6301 tatgcatcgg ccatataacg gttcttattt aaatcataat ttgattcatt ccattcaata
     6361 atctcataat cagggcaata ctttttccag ctttctatgc actttctatc ttttttcgga
     6421 agtggatttc ccccgaacca acagtaatgt atcttcttag gaatcataat atctctcctt
     6481 ttgtattttt ttcactataa taatgctctt tccttttcac aacttcaggc atggctaaaa
     6541 tcatataaat cagtggcccc gttgtttcac ccctaagtgt tgtgttatac acaaaaaaga
     6601 ataccgacat aattgcaata actttaacaa gacttgcatc tttatccttc ataaaagatc
     6661 tttttgcatt gaaaaatgtc tttattataa aaaataggaa tatcgaaact cctaatattc
     6721 caacttctat aaataaccaa atatgatgaa aatactgata atgaagatta ccaaacgatt
     6781 tataaaactc actgacaaaa aaattggtaa actctgcatt gccaatgcct actcccatta
     6841 ggactaatct aaaattatta ttaaatattt catctactac tctagagata ccagttattc
     6901 gatttaaatc accaacacca tcatatccag aatctcttga cgcatattct aaaagacctt
     6961 gaattgtgaa attatcagca tagctttgtc cattatcgtt caatgtaata aatattgtta
     7021 taaaaaagga taatactatc gacacaagta ttacgcatga taatatcttg atcactactt
     7081 taaaatcaac ttttaaagtg aatactattg caacaaatat tataaataat tcaggaataa
     7141 aaactttcaa ttcagctaat gcagacataa aaagtgctgc acaaacacat aaaataaaat
     7201 ggatgaaatt tttttctccc ttgaaatatc cgattgtaac aacagtaact gtcacacaca
     7261 aaattaaatt caatggcata ttacaacctg aataattcca aaaaactcca cctatcatat
     7321 ctcctacaat tgttccagtt ggataggtga ccatgaatct ttcaattaaa caaagtggca
     7381 gtgaaatcca gaataatttt tcaattatag cgaatacttt atagtaatct ttaattgtta
     7441 aatatgttgc acaatttata tatactatga acatacgagt gttatttcgt atcccccaga
     7501 taatcaatcc tatattttct aaattaataa ctgagctaca aatagaaact agtaaaaaca
     7561 aaactatcag catctcattt ttgctagcat atattccttt tctgcgtcta aagctaagtg
     7621 catttaataa cacaaagata ttaattatat ctcccaaata taaaattgct cttggggcgt
     7681 taaaagtata tacaagaact atggagattg caaaaaatgc caacgtgaac aatgtccaat
     7741 ctgctcctga ccatcttttt attgaaatac gcatcgttat tcaccttctt tcgctaaaaa
     7801 ttattctaaa aatacttttg ttaaattaac ataccctatt atttttgccg gtacacctgc
     7861 aagaattgca ccattctcta gacaatcttt gataacaata gctcctgccg caacaactac
     7921 attgttagct atttttacat taccaataat ctttgcaccc acgcatacac gtacattatt
     7981 tccaattaca ggacacttag gatcgattcc atcatttcca atacagttat tcccatataa
     8041 gtaacaattt tttcctatta tggcgttacc attaataaca attccttgtg tatggtaaaa
     8101 tcttacacct tcatcaaata cattttcacc acactctata ccacattttc ttcccaaact
     8161 attcattctt ctgcaagaca caatatacat aatcgcataa atgatatttt tctttctctt
     8221 tgaataataa taagatgtaa ttctcattcg acgagtatac cgccaagcgt tgtagtccgg
     8281 atgtgatttc attattttta gtaattttgt gtgttgtcct ttagattcta tatatagatt
     8341 cttttccata cgaagaaccg cttttaaata ttctttactg ctaatttcca ttcatatctc
     8401 ctattaattc aatttatact taacaatttt attcggattt cttgcaacaa gagcatcatt
     8461 caatgctact gaatctacaa cgatggatat gttatcttca tcgttgattc caccggatac
     8521 aatattactt ataaaaatcg taacattttt tccaaaagaa gggatgtccg tgatgtataa
     8581 tgcccatttt ccgatatttc cagttattta aacctaacga atttttacaa tagtacaagt
     8641 atcacaatca ttaatatata actggaatat aaatctccaa aaattcacta tagtacagtt
     8701 ttttatgatt aaaatctcct atttctaccc attccgctta aatatgctaa tgttttaacc
     8761 ccaataatgg attggtttag taaaaaatag ttaaatattc gttgtttaat tggattttgt
     8821 ttatatgggt atgagtgaat acaggcaatc aactcatcgt tcataagtat cttagacaaa
     8881 taggagttta aatcaaattc tttattaaaa ctagcatatc taactgcctt ttgaatacaa
     8941 gtacgacagc gtcccaacaa aagtctcctt gttttgttta caaatatctc attttctaat
     9001 atatagctta aatcgccttc aattttttta actaattcaa tattacaatc gaactcatta
     9061 tctctaaaat tatgtgtcaa tgaattatta ttattacatc tataataata gatacaatct
     9121 ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa tatggaatat gatatcttca
     9181 gatatataat ccctttctga cggaaacttg atattgtgct gttgaattat ctcagatgaa
     9241 tacagcgcat gccaaactga cattggcaat ataaaatcga ctttataact cggctcagtg
     9301 ccaatcattc caagcaacac tttttcaatt atagcgttat ttgtaaatat cgtactatca
     9361 tatcttgctg gctttgaaat aatattatta tcatcataat attcataata tccagcataa
     9421 catgtgtctg cattactcct ttttatacca tttaacattg cttcaatata attagctgca
     9481 acaaaatcat cagaatcaat aaaagcaaca tattttcctt ttacatgttc aagtcctgta
     9541 tttcttgcca atcccaagcc agcattattt ttatgaataa tacgaatcct tgaatccttt
     9601 aatgcatagt cttcacatat tttattagat gaatccttag atccatcatc cacaattata
     9661 atatctaggt tcttatatgt ttgattacac actgattcca aacaacttcc tacatacttt
     9721 tcaacattat atacaggtat gattacacta attagatcac tcgaattcat ccttctatcc
     9781 tttcactcac gttttctcaa ttagtcaatg acggcatcaa cgattttctt aaattctctg
     9841 aaaaaaatag actctgatct tgatgatgtg aatgacatac tatcttcttc tagaaaaatt
     9901 atttccttta aattatttat taaatcaata atatgatcct cgtaagtatg ctttcccttg
     9961 gaataatgaa aatatacttt tgtattacac aatattgcaa ttttatttct taatatgatt
    10021 atttcactca ttcattactg ccacatatac acaacttata accaccccct gcaaagttta
    10081 gggtaagtta tcagataata tcttcctctt catcatccag aatacaaaat ctatttattt
    10141 atagaaatat cattaatgtt tttatataat gtaccgtgct tgtactcttg ctctattaca
    10201 agcactaaaa cactactatt catttgaatt cttacacgga taaaaattta catcgtctat
    10261 ttcaatattt tttctctcta ttttattcac ttaataagtt cattacataa tcgaaacatt
    10321 ttttattttc cttgtcatca ctccttgctc cctttttgat acagatttaa aattctatca
    10381 aagaaatttc catcgttaaa tttcgttatt gccaaatcct tcgaacaacg tgaaaatttt
    10441 acgtatttat ctttatcatt gatttttagt attgcagatg tcaacgctga tacgtcacca
    10501 ggttgaaaca aatatccatt ttctccatca cgtactgctg aagaaatatc tcctacatct
    10561 gtagcaatta caggcattcc ataagaaatc gcctctagaa tagatatagg caatccctcg
    10621 ttataggacg gtaaaatcat cacatttgat cttctatagc actcctcctt tttttctccc
    10681 acaacccaac ctgtaaattc aaaatacgat tgcaaattat attcttctat caattctttg
    10741 agcgatattt cttcatcacc tgctccagca atctcaaaaa taaacttttt ttctggatat
    10801 tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta caccttttcg ctttatcaaa
    10861 actcccataa acagaaatcg gcacattttt gaattccact ttatggtata ttctggaact
    10921 acaatagaat tttctacaac aacagtttta caatttggct caatttcttt aattatcttc
    10981 ttccattttt ctccaagtac aatcgtggaa tcagactctc ttaacagcaa gcgaatctga
    11041 ttttgttttt ttacagaaac actatcatac cattttttaa attccgatcc atgaagatgg
    11101 atgattacag gaatatttaa taatctaaac cacttattca gcacatattt cctactaaaa
    11161 ctccctctgt acgacatgtg catatgaata atattaggtt tactcaataa agagatacct
    11221 ataattttag caaatgcata cataaaatat aaagatttaa atattgcatt tccaccctta
    11281 taactcggta taaaagtcat ctcgatattc tttttttccc aattaaaatt gagcatttgt
    11341 gtaattatac ttgtaattcc accttttacc gagctgtcat taccaatcat aagtactctt
    11401 tgcataacgc tttactcctt atatttatat tttttctatt aatacctcta tcgaattaat
    11461 aatcgtttcc gtgttagata catacttgtt aaacatccga actcttgttt ctttaacggc
    11521 atcttctaaa ttttctaatt cataacaagg atagataata ttcatctcat caaattgtct
    11581 aattaactgc agttggtgat catcgacatg ctcaccatac ttagccaatc gaggaacagc
    11641 aatgaccctt ttaccttttt ttacggcccc aattatagca cctgtacctc catgagtaat
    11701 caccatttct gctttttgca tagtttcagt aaactcatcc ctatttagaa atgctttata
    11761 ggaataattt agtggtttat aatcactata accactctgt gcaaatgtac tttcgattat
    11821 tactcctttt tcaattaaat catcaatagc ttttaacaat ctatcaaatt gaaatttttg
    11881 tgaacctaat gttataaata tcattttttt ctcctattat ttaatatatc ccacccaaat
    11941 aaatagcatt aggataaaat tgtagcatag tttcccactg aacataaaat tgatctgcaa
    12001 aatgatacat caattttcct gtctgagtag ggctggttac ttttgcaaac gattcaatat
    12061 aaatcagttt ttttcccatc atttttgtta ttaagcagat agggatcata gctaaaacac
    12121 ctgtacaaat cacgagatcc ggtttttcct ttatatatat aataattgat ttgaataaat
    12181 taataagtat agtacacaaa aaaaactttt cttttctatt tacttgtctc atgtagtacg
    12241 ttttttcgcc ttttgactca gctgaatatt ctgtctgttc tgttataaca aaactctcat
    12301 acttctccat cagtggtttt aacatcatta actgttcata atgcccccca gaggaagccg
    12361 caaaacatat ctttttgttc atattaacct ctctatcagt cacgctgaaa aatatctcta
    12421 gtgtaaactt tttctttaat atcatccaag caggtatcat atcgattggc aataatagaa
    12481 tcacacatcg ccttaaactg ctctaaatcg ttgataacct tacttccgaa aaaagtctct
    12541 ccatctttca gtgcaggctc ataaatcacc acagtcgcac ctttagcttt tacgcgcttc
    12601 ataactccct ggattgaact ctgacgaaaa ttatcactgt tgctcttcat ggtcagccgg
    12661 tatataccga ttaaaactgg attttccctg ttgacatcat actgattata ttcagcgtag
    12721 ttgtaatagc ccgccatctg aagaacacga tctgcgatga aatccttacg ggtacgatta
    12781 gattcaacga tagcctcaat caaatttcca ggaacatctg catagtttgc caacaactgc
    12841 ttggtatctt ttggcaagca ataacctcca tatccaaagg atggattgtt gtagtgagtt
    12901 ccaatacgag gatcgagaca aacaccattg attatctgct gagtattcag ccccttcatt
    12961 tcagcataag tgtctaattc attgaaatag ctgactcgca gtgctaaata ggtatttgca
    13021 aacagcttta ctgcttcggc ttcagtgaat cccataaaaa gagtatccac gttctcctta
    13081 attgcacttt tctgcagaag tgttgcaaaa acatgagcag catttaacaa gcgctcatca
    13141 gtcatatcag taccaacaat aatacgagat ggatacagat tatcatataa agccttgctc
    13201 tcgcgcagaa actctggact aaaaataatg ttcttactcc ccgttgtctc acggactgat
    13261 tcagtgtagc caacaggaat tgtagacttg atgaccatga tggtatcagg attcacgtcc
    13321 atcacaacct caataactga ctcaacagca gaagtatcga aatgctgagt acgactgtca
    13381 tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt tataagcgct ccttgcatcc
    13441 aaagtagcag ttaagtcaag ttctttttct gtaagatact tttcaatata ttcatcctga
    13501 attggagact tccgtttatt aatcagttca atcttttcag ggataatgtc taccgcggtt
    13561 acctggtgat gctgcgccaa tagtgtggcg atactcaaac caacataacc ggtgccggct
    13621 actgcgatgt tgtacttttt cttcacaata tctcctcttt cctctcttaa gtatggagaa
    13681 tgttactcct acggctattc atcatgtcaa aataacacag cttagaatgt tgataaaata
    13741 attttccaga atgtgataaa atctctttca tttataaacc acacatctaa ttttatctat
    13801 tctccacaag acatgtagag tattctttaa taacaagata cgaccttact aaatacttca
    13861 aaaaattatc atatttaatt cataaatata aatcttcatt ttaaatgtat gcccaaaaat
    13921 ttatctgtaa tgcgtcatca aaatctagat tccaaatctt tatcaatttt tatccactca
    13981 ttctaccact ttagtaatat tttctattta taacactttc gtcctacatt gatccccccc
    14041 tctgaagtac agtttttact gtcttaaata aaattttaaa atctaacccc atactccaat
    14101 tttttatata ttccgtgtca agttttgcta cttcttcaaa atcagtaata tcacttctac
    14161 cacttacctg ccacattcca gtaattcctg gcttgatagc aattcttgca cgatgatgat
    14221 aatcatattc ttcatactct gatttaaggc atggtcgaaa tcctataagg ctcatctttc
    14281 ctgttagaac gttgaaaaat tgagggaatt catctaaaga cgtggatcta ataaattgac
    14341 cgattcctgt cttttttgtt ccatctggaa gtattttatt tccgataatt cttggatcaa
    14401 actccatctt aaacataaga ttattgctca tcttattttc tttcattaat tctacttttc
    14461 tttcttctgc atccatatac atacttctaa acttatacat cttgaacggt ttaccattct
    14521 gacctatacg ttcttgtgca aagaaaattg ggcctggtga attaatataa ataagtggag
    14581 caataaagat aaaaataatt cccgttgcaa tacatccaac aatacctgca agaatatcaa
    14641 ttgttcgttt tataaatgcc tgttgagttg tcataacatt gatacttgtt gtaagtactg
    14701 tatattctcc tatacgacca atagattgtt ttcccaaagc actatctgat acttttgcaa
    14761 gattcaaatg tacagtaagg cccatttcag agcatcgttc aataaattct ttcatacacg
    14821 aagtactttt attcaaaata ataaagactt catctaccca tccctgacat aagtaatccg
    14881 ctgcattttt ggaatcagaa acaactgtca ctccctcaac atcttcacca atcatgcttt
    14941 tatctatgat aatgacacca tttattttat acattttata gttatgctct ttcacattac
    15001 gaataactac ttcagcaata ctactagatg taactataat aagtgatttg ttcccctcat
    15061 ttttcatttt atgtttcaag taatgtttcc ataagaggcg agttatatat gtaattacaa
    15121 cgtataaacc tcccatcaag aaaagaacga ctctagaata atcatttcca ccctgaacac
    15181 taatgagata caaacttcct aacagcacca acataatcgc ttgctttaca gaagaagcaa
    15241 attctatata gtaccctctt tttaatacac ctctatatgc ttcaaacatg aaaataacta
    15301 aaagatctgc cagttcaatg aagactgcca tgttttgata cattggaatc acataaggat
    15361 tccaatctac ctggcgtaag acatacgata gataaaaagc aagctggata cacaataaat
    15421 ctaaaataat aaaatcaata tgtttaaccc atccccttga acttttcttg tacattcctt
    15481 tttactcccc cctcttttta ataaaataaa ttgtattact tgttttctct cctaaatatc
    15541 tttctgaagg attattgact cgacaaattg gcttgtggat attcttatat atagaacatc
    15601 cgtaagccaa cttaaaactc cttaacaaaa tttttttact catattgtag atcaaaacaa
    15661 gtatccaaga aatcatattt tatttctttt ttctgcttac tcaacaacag ctaaaagaaa
    15721 aatcatcata aacaaattca actaagccaa cgatcccctt gtgaaatcaa gttttttctt
    15781 gatttacgaa taaagagcta aaataaacca aacttcttct tttttacctc ttcatagact
    15841 ggataactga tttcatcccc gttgatcaga tcctttgcga cttgtttcat ctgtactact
    15901 ttttccttac caaagtcctt tgcaatctgc tcatagcact cttttaagta aaaggtgcgc
    15961 ttagagaccc catgggcatc agaagcagcc atctgcacga gattatgttt gaccatcgtt
    16021 ttggctgttt tctggatgtc tttgccaaat accccaacat aacttggcgc agtgagttgg
    16081 gccaaacatc ccatatctag gaacggaagc aaatgatttg ggtcttcgcg aaaatgcacg
    16141 ttgcgttctg gatggacgat tactggtgtc tttcctagtt ctaacaactc aaaaaaaagc
    16201 cgttctgcat ataacggcac ttccagtgtc ggaaattcga tcagaagata tgtatcatct
    16261 aaatctgtaa ataa
//
LOCUS       DAYLZO010000067        15629 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.068, whole genome shotgun sequence.
ACCESSION   DAYLZO010000067 DAYLZO010000000
VERSION     DAYLZO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.068"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            2..74
                     /locus_tag="ACGVJG_001897"
     tRNA            2..74
                     /locus_tag="ACGVJG_001897"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACGVJG_001898"
     tRNA            78..150
                     /locus_tag="ACGVJG_001898"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACGVJG_001899"
     tRNA            155..225
                     /locus_tag="ACGVJG_001899"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACGVJG_001900"
     tRNA            238..311
                     /locus_tag="ACGVJG_001900"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACGVJG_001901"
     tRNA            323..394
                     /locus_tag="ACGVJG_001901"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACGVJG_001902"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACGVJG_001902"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFJ6557831.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACGVJG_001903"
     CDS             1983..3455
                     /locus_tag="ACGVJG_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFJ6557832.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACGVJG_001904"
     CDS             3538..4326
                     /locus_tag="ACGVJG_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFJ6557833.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACGVJG_001905"
     CDS             complement(4393..5061)
                     /locus_tag="ACGVJG_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFJ6557834.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACGVJG_001906"
     CDS             5176..6888
                     /locus_tag="ACGVJG_001906"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557835.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACGVJG_001907"
     CDS             6891..8663
                     /locus_tag="ACGVJG_001907"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557836.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..9866)
                     /locus_tag="ACGVJG_001908"
     CDS             complement(8745..9866)
                     /locus_tag="ACGVJG_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6557837.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(9938..11218)
                     /locus_tag="ACGVJG_001909"
     CDS             complement(9938..11218)
                     /locus_tag="ACGVJG_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFJ6557838.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(11309..11761)
                     /locus_tag="ACGVJG_001910"
     CDS             complement(11309..11761)
                     /locus_tag="ACGVJG_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFJ6557839.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(11774..12184)
                     /locus_tag="ACGVJG_001911"
     CDS             complement(11774..12184)
                     /locus_tag="ACGVJG_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6557840.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            12459..12671
                     /locus_tag="ACGVJG_001912"
     CDS             12459..12671
                     /locus_tag="ACGVJG_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFJ6557841.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            12674..12886
                     /locus_tag="ACGVJG_001913"
     CDS             12674..12886
                     /locus_tag="ACGVJG_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557842.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            13026..13181
                     /locus_tag="ACGVJG_001914"
     CDS             13026..13181
                     /locus_tag="ACGVJG_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557843.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(13178..13402)
                     /locus_tag="ACGVJG_001915"
     CDS             complement(13178..13402)
                     /locus_tag="ACGVJG_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFJ6557844.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            13480..13668
                     /locus_tag="ACGVJG_001916"
     CDS             13480..13668
                     /locus_tag="ACGVJG_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557845.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            13665..13838
                     /locus_tag="ACGVJG_001917"
     CDS             13665..13838
                     /locus_tag="ACGVJG_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557846.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            13807..13935
                     /locus_tag="ACGVJG_001918"
     CDS             13807..13935
                     /locus_tag="ACGVJG_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557847.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            13994..14329
                     /locus_tag="ACGVJG_001919"
     CDS             13994..14329
                     /locus_tag="ACGVJG_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557848.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            14562..15503
                     /locus_tag="ACGVJG_001920"
     CDS             14562..15503
                     /locus_tag="ACGVJG_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFJ6557849.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            15505..>15629
                     /locus_tag="ACGVJG_001921"
     CDS             15505..>15629
                     /locus_tag="ACGVJG_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFJ6557850.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEV"
BASE COUNT         4912 a         2535 c         3078 g         5104 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtatctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt gttttcttca
     9061 attaattgat ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct
     9121 gtatttttag ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt
     9181 ttattaccat cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtccagtt
     9241 aaaaacaaaa atttagtaat taaagaataa cgtgactgtt gatgtgaact tttttgttta
     9301 ctagcaatca tttgaacttc ttcttttgat aaataaccat ctttttcttt ttttatttct
     9361 tcgtttgtct taggttttct tgctaaagaa accccattta cgggattata ttttaatcca
     9421 tatcgacggt tggcgaattt aaaaatcatt cctgtaaaag tttttaaatg tattacataa
     9481 ttatatgata aatttttttc aaaatagtag taatctagca tatcttgtat aagattacta
     9541 ttaacgtttt ttacaagtac atcatctttt atatattttg aaattaatga tagataaacc
     9601 tgagttgttc gataggaact ttcttttaca gttggcttgt atttttcatc ccatttagtt
     9661 tttaattctc caaaagttag tggtaattct tcatttcgtt ttattttttc tgcgattttt
     9721 tcattcaaaa gtaagttagc ttgattccaa gcttggcgtg aattgctaga taaagtaacg
     9781 gacacttttc tctttttttc agttaaggga tccgtataac gttcggtgta cttaaatttt
     9841 ttgtcattta ttttttctaa ccacattatt tctattcctt tcgaatatac gttcctatta
     9901 attgttaaaa gaaaaagccc gaaggctttt tatatattta atcttttgac aattctaaag
     9961 atcgtctata ttgttcagct ttagctaatt ttgtaaatgt tactgttcca ttataatttt
    10021 ctaaaacaac tttctctatt tcgtctagtg aaactttgaa aaattctttt ctttgattaa
    10081 ttttattaac ttcttgtttt ctaaaatgat tatgcaattt agtttccaaa gacggagcgt
    10141 cctctgaaaa aatcatcgca tgaacgtcaa attcaaatgg aaccgatgca ctactcaatt
    10201 cttttattct atccataggt tctaatcttc tagtcatacc aattttatat atgtcttcac
    10261 caaaagaccc aatattacta attacataca cataaccggc tctagtattt aattctcttt
    10321 gtaatacatt ttcacgtata acttctaatt cttttaattt ttcttccagt tcttgaattt
    10381 tatttgcata taattctttt tccacatctg cattagcttt ttgcatatat gcgattaatt
    10441 tggaaatttc agaattgaat tgacgttgtt ctttatcaat acgtttcttt tcacgatcaa
    10501 tttctctacg aactttttcc tcttctatca tctgttcttt tattgctttt tgttgttctt
    10561 tttcttcggt aattttcact aaataagagt aatgaagttc tagttcttga agtttaaact
    10621 ctaaaagacc cttccttaat tcagcattgt cattcttgaa taaattatta attttatcaa
    10681 atatttttat gattttttcc ctagtcgagt ctatattaga aatagttagt ttattgagta
    10741 atcctttcac ttctgaatca aatggaatga gcattttttt ctttaaatta tttttctctc
    10801 gtttatttaa aataagaggg acagaaacag catcgtcaat aatatattcc ttttctttta
    10861 attttaacaa agaaagctta ttttttattt cagaagatga agtttcttct atatttgaaa
    10921 cgataagtga agaatagaca ttttttaatg tttcatcata cttttgattt atttcatcat
    10981 ttaaatcttc tttctctttt tttaataaaa taatatcatt ttttaacata gtaatttgtt
    11041 tatttaaatc tgatagtgtt tcttccttac ttaatataaa tctttctcta tcatgtaatt
    11101 tatttagttt ttcatcattt ttttgttcta attcataagc ttcttttttt attgtgttag
    11161 ctttcgacag tttagtgatt cctataatta ataaaataag tccaatcaat aataacattc
    11221 aaaactcctc atttctttga tataattatt gtagagttta ctcagaaatg aggaaagagt
    11281 ccatgtgaaa gcatgggctt ttttattttt aatgttgtaa tactaaatgt tcaagattat
    11341 gaggaagccc taaatattca ataatttgga actgactcat atcagataaa tcttgttcat
    11401 ctattgttct agttaacatt tcaattgcaa acatattagc ttcagcttct ataccataaa
    11461 tcatacaact aaggttgttt gctctaaaag ctgctgtgca aataccttta tggagtctaa
    11521 cgtgggctat ttcatgccat aatacaaatc ttttcataga aggtgatagg ttttgattaa
    11581 ttaatatcac ataacatctt ttattgtgta tcgtttgtcc taatgtttta ttccctaaat
    11641 cagtttcaat taaatctata tcagaatatt tgattatttt atatggatta atcgcatcag
    11701 atccaaactc tacaaataat ttttctattt cttctaatat ccagtatttg tttaaatgca
    11761 taccaaagcc cacctattga cgatattttt taggagtgaa ttttttcttt gctttaactt
    11821 tggttaaggc aagtgcaagt tgaatggcat cgtataattt ttccttatct tcatcgtcta
    11881 aaggttcacc ataataattt gtttcagcat ctgaattcaa gccttcaagt aatttttcag
    11941 cttgtaaagc aatatcttgt ttatcttttt ctgttaaatc ataataatgg cgtttatcag
    12001 tacgtcctaa aagataatca gttgatacat taaagtaatc tgcaactttt tgtattttat
    12061 ctccagatgg agtactttta ttccatctag acatgcttcc atttccgaaa tttaactttc
    12121 tttcaagttc tgccaacgtc attgaacgtt cttttaataa gatttttata cggtctaata
    12181 gcataggata tgcattcctt tcaaaatacc gacaaaaaaa gtatttttct gcaaattatc
    12241 tatttacaaa tagagaattt gcagttatac tagttttgta agcaagatat ttacggcata
    12301 caaagactaa aagcaataaa ctaatagtct ccccagacat cagtaagctc gctttttgtg
    12361 cgctgtattt tgtatgctta cataatagca tatttgcagt ttttgtcaaa cttattagac
    12421 aaaaatatta gaaaatattc gataaggggg tatgaagcat gactttaaaa cagcaagtat
    12481 taattgcttt agtaaagaaa ggttggagtc aaagagagtt agctcgacga atgggtatat
    12541 caattactta tttacgagat atcttcatcg aaaaaagaaa acctaaggaa cgtttaaaac
    12601 aaatcgaaga acttttggat atcaagttag aagtagattc taatgatcag ccatcagaac
    12661 aggaggcgta aaaatggtac aacgtttgag caaagcggag tttaaagaga aatataagta
    12721 ttctgaaagt acttatcaaa gaagaatgaa ggaatttaaa aaatcacgtt ttagtgaggg
    12781 gtataacgca gttacttcca aagaaataac aattgatgtc gatttatatg aacagtttag
    12841 aacatggcgt tctaaaaatc gatttagagt tagagctgtt tgttaatagc taaaaaatct
    12901 aaattcacca cttctgaaaa gagaagttgc tgctctcctt tcaagtttgc caaagtccac
    12961 gttttttcat tttggcaact cctcttcttg gaggtggtga cggcgagaga cgaaggaggg
    13021 atgacatgaa ggaagaatca aaaataggga ttttgaatga gaacaattac acaagattaa
    13081 ttattgaaac agaagaaggg aaaaaagtag cagaagttac attgaccgat gcaactcctg
    13141 ctgacggtta tcgtattcga ttaactccta aatatgatta accttttggt gggtgtggat
    13201 cgtttccgtg actatcacgg ctttgaattt ttccatctct gccatgaata aatagctcag
    13261 atccttgatt tttagaaatc tctctagcga tttttactgc ttctgatttt gtattagtat
    13321 gtgcagtcgc tttagaattt ccagcacctt tgacattcca accaccattg gaattaggta
    13381 caacatgttg attttttccc atagttttca cctccatttc tatagaaata ttttatcaaa
    13441 tagaaatgga gaaattcaat aaacgaaagg agaagtgata tggcatatac cttgcaacaa
    13501 gaacatcaaa ttctcggttt gattaaacaa cgcaggaaac aattacaaga tgaccgtgca
    13561 gcgcttagaa aagccgatga gctatcagat aggcaagctg aactaattgc ttctgaactt
    13621 gaggatttga gaatgctaga aataaaaaat agggagatta gattatgaag aagacagaca
    13681 cactttttat aggattcatt ttggggttat tagtgattgt agcgcaccaa agtattatcg
    13741 ggggaagctt gttcgcagca ttgatggttt taatcaatct gcttgattca aaagaaagga
    13801 gcaactatgg cacgagaaga agcgctaaaa atcggtaaag tgattgctga taatcggtgg
    13861 ggaaatagcc gccctattat tttaagcaag caacatatcg aaaagcaaaa agcatggcaa
    13921 caaataaaaa agtgactctg ccagccagca aagagtcaca tacaaattta taaaactagg
    13981 agaattatat cacatgaata agaaaattga aaatcttatc gaagaattga aacgtgaatg
    14041 ccaaaagcaa ggtgtttcta ttatttgtac agcccaaaaa gaaggggaac ttaaaagtct
    14101 cgtttatggt gagacaacag aaattttact ttgtcttgct atgcaagaag aacatttaga
    14161 tgaaaatctc ccattgtcag cacatattat gcgtagaatt gctgtggatg cttacgaaca
    14221 agcgaaaaat gaagaagaaa atcaaccttc aaatcatact tttgttatta ataacaagga
    14281 agatttagct gatgtgatga ctcgtattct caagggggaa tttcaatgat gaatgaaaaa
    14341 acaattgatc tactcattat tttatttttc acgttagtga ttgtagcgaa aagttatgta
    14401 atctttggct tgttattcgc aattaccatg cttgtgcttt gcgtgttagc aagcagaaaa
    14461 gaggttaaac atgaacgagt tagcaaatct tgacaactat ttaactgatc ctgattatgc
    14521 taaaccacca tatgaagcac caattgatga ggaggatgaa gatgagtaaa tctaccttag
    14581 aaatgagcca tcaagaatgg cttgaagacc gtaagaaagg catcggaggt tcggatgttg
    14641 gaacaatttt aggattgaat aaatggaaat ctccttatca actatggcta gaaaaaacag
    14701 gacaagtagt acttgaagaa tcaggaagtg agcccgctta ttggggtaat gttttagaag
    14761 aagtggttgc taaagagttt caggaacgta caggcaaaaa agtacgcaga agaaaccaag
    14821 tatttgaaca tccattacat ccatttttaa gagcaaatat tgatcgggaa gtagtgggag
    14881 aaaatgccat tctggaatgc aaaacagcca atcaatttct cggtaaagaa tgggaaggcg
    14941 aagaggtacc gcttagctat ctctgtcaag ttcaacatta catgaacgtt ctaaacaaag
    15001 actattgtta catcgctgtc ttaatcggtg gtcaaaaatt tatctggaag cggattgaac
    15061 gagatcaaga gctgatcgat acaatcactg aacaattagt agagttttgg gaaacaaacg
    15121 ttcttggagg tatcgagcca attattgatg gtagtcaagc gacagctgat ttcttgaaag
    15181 agaagtatgc agatgttgaa gacgttcaaa cgacgttacc aattcatttc gatgaactag
    15241 ttgaacagaa aaacgaactc aaacggacta agaaagaaat tgaatcagct atccgtcaag
    15301 tagacaacga gattatcagc gagctaggta aacgtgaagc cagtatcggt atcactcaaa
    15361 aaaacatcat cagttggaaa tttgtccgta cgagacgtat gaactcgaag aaactagcag
    15421 agaaatatcc agatgtcgca aatgatgaag agatttataa cgttactgaa tcaagaaggc
    15481 taaccgaaag ggagatcaaa taatatggca acaaatgaat cgttaaaaaa tcaattggca
    15541 gcaaagccac agaaacaagt tgcaccaggt caattaggac ttaaagcttt gatgaataca
    15601 ccaacaatga gaaagaagtt cgaagaggt
//
LOCUS       DAYLZO010000068        15404 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.069, whole genome shotgun sequence.
ACCESSION   DAYLZO010000068 DAYLZO010000000
VERSION     DAYLZO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.069"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..934
                     /locus_tag="ACGVJG_001922"
     CDS             <1..934
                     /locus_tag="ACGVJG_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFJ6557851.1"
                     /translation="KLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSA
                     NASKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGK
                     KILDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKD
                     TTKEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRC
                     MLSKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRI
                     IAVSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            complement(1063..1926)
                     /locus_tag="ACGVJG_001923"
     CDS             complement(1063..1926)
                     /locus_tag="ACGVJG_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFJ6557852.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(2713..4005)
                     /locus_tag="ACGVJG_001924"
     CDS             complement(2713..4005)
                     /locus_tag="ACGVJG_001924"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFJ6557853.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            complement(4785..6152)
                     /gene="dnaB"
                     /locus_tag="ACGVJG_001925"
     CDS             complement(4785..6152)
                     /gene="dnaB"
                     /locus_tag="ACGVJG_001925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFJ6557854.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            complement(6426..6878)
                     /gene="rplI"
                     /locus_tag="ACGVJG_001926"
     CDS             complement(6426..6878)
                     /gene="rplI"
                     /locus_tag="ACGVJG_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFJ6557855.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            complement(6884..8857)
                     /locus_tag="ACGVJG_001927"
     CDS             complement(6884..8857)
                     /locus_tag="ACGVJG_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFJ6557856.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            complement(9000..9236)
                     /gene="rpsR"
                     /locus_tag="ACGVJG_001928"
     CDS             complement(9000..9236)
                     /gene="rpsR"
                     /locus_tag="ACGVJG_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFJ6557857.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            complement(9262..9774)
                     /gene="ssb"
                     /locus_tag="ACGVJG_001929"
     CDS             complement(9262..9774)
                     /gene="ssb"
                     /locus_tag="ACGVJG_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFJ6557858.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            complement(9824..10123)
                     /gene="rpsF"
                     /locus_tag="ACGVJG_001930"
     CDS             complement(9824..10123)
                     /gene="rpsF"
                     /locus_tag="ACGVJG_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFJ6557859.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            complement(10359..12830)
                     /gene="gyrA"
                     /locus_tag="ACGVJG_001931"
     CDS             complement(10359..12830)
                     /gene="gyrA"
                     /locus_tag="ACGVJG_001931"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFJ6557860.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            complement(12850..14796)
                     /gene="gyrB"
                     /locus_tag="ACGVJG_001932"
     CDS             complement(12850..14796)
                     /gene="gyrB"
                     /locus_tag="ACGVJG_001932"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFJ6557861.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            complement(14793..>15404)
                     /gene="recF"
                     /locus_tag="ACGVJG_001933"
     CDS             complement(14793..>15404)
                     /gene="recF"
                     /locus_tag="ACGVJG_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HFJ6557862.1"
                     /translation="QTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQIT
                     HQKEQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFF
                     INQKNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLL
                     ETIEGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
BASE COUNT         4221 a         3338 c         2816 g         5029 t
ORIGIN      
        1 caaactttat gtaggaaatt atgatttttg gttggaatct agtcaactag cggctaaact
       61 tcaagcaaac gcaaatgcca aaaaagaaga acaaatcaaa gaactccaag aatttattgc
      121 ccgctttagt gcgaatgcat caaaatcaaa acaagcaact tcacgtaaaa aaatgcttga
      181 aaaaattact ttagacgata tccaaccttc ttctagacgt tatccattcg ttggattcaa
      241 acctgaacgt gagattggaa acgacttatt gcaggtagaa aatgtaagtg taacgattga
      301 tggaaagaaa attttagatg atatatcttt taatttgagc aaagaagaca aggtcgcttt
      361 cgtcgcagaa aatgatataa caacaacaac gctattcaaa gttatcatgg gtgaaattac
      421 gccagatact ggaacagtac gttggggtgt gacaacaagt aattcttatt taccaaagga
      481 tacaacgaaa gaattcgata ctgatctaac gattttagac tggttgcgtc aatatgcaag
      541 taaagaagaa gatgataaca ccttcttaag aagcttctta ggaagaatgt tgttttctgg
      601 tgaagaagta ttgaaaccag tgaatgtttt atctggtgga gagaaagtcc gttgcatgct
      661 ttcaaaaatg atgctttcga aagctaacgt acttgtatta gatgatccaa cgaaccattt
      721 agacttagaa tcaatcacag cattgaatga cggcttgatg gcatttactg gctcaatctt
      781 atttgcgtca catgaccatc aatttatcca aacattggca aaccgcatca ttgccgtttc
      841 ggataaaggt gtgatcgatc gagctgatac gacatatgat gaatttttgg aaaatgcaga
      901 tattcaaaaa caattgaacg agttatgggc ataatggcct atttagctta aaaaataaaa
      961 aaacagcgac ctctaagaga taggatgctc atctatcttt taagagtcgc tgtttttgtt
     1021 acaatcaatt tctgggcttt tttacagaat gagttactgc ttttatcgac tgattcccat
     1081 cgaaaaaatc gcgatacagc catagcctgt gtgactggcg atcgttggtc ctaagggata
     1141 aagcttcaca tctttcatct ttatctgctc ttccaaaagc cgttttgcct tctccgcgct
     1201 ctctagatcc ccagcgtaag ccacataaac gatttgatca agtggtgcaa cgattgtttt
     1261 taccgtctca ttgacgattg tctggatcga cttgtttctt cctcgaactt tgtcaacatt
     1321 cgtcagcctt ccttctttat ccaccgtaat aataggcttt acactcaata gactgccaag
     1381 agtagcactt gctttggaaa tcctaccgcc tcgttctaga tatttcagat cgtctactgt
     1441 tacccatgaa tggactttca aataattact ttccagccac tctagggttt cttcgatgct
     1501 tttaccttct tttttcaatc ttacagcttc caatactaaa agcccttccc caatacttgc
     1561 agcttttgta tcaaaaacat agatctcagg gtcttcatat tcttccttca gcaattcaac
     1621 cgcttgaagc gcactactgt aagagccgct taatccactg gaaaaagcca catataaaac
     1681 aggaatctgt ttttccgcat aagaacggaa aaattctaaa tatcggccaa cattgatttg
     1741 tgatgtcgta ggcatcgcac catttttcag ctgattcatg tagcggtcat aattgaatgt
     1801 ctttccgagg tcatctacta actcttgatc attcaattgg agattcattg ataaaaaatc
     1861 aactttttct tttgctaata catggtatgg cagatcacaa caagaatctg ttacaatttg
     1921 atacacattt ttcaacccct tctcgttaca ttttaccctt atcgtctatt atagcaaatc
     1981 tcttgcgcta gacacacttt aaccggttta ttttcgcaag ttctaatcaa aataagaggt
     2041 tgtgacagaa gtggtcagct tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa
     2101 tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg tgaatcagag
     2161 gttgtgaaat cagcgttttg acctgagtat tagagaaaaa aacggaaaaa atagttcctc
     2221 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttaccaag
     2281 aggttgtgag cctgccgttt agctccgagt cacaaggaac aatttcaaaa aacagcttct
     2341 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttagtg
     2401 gagaggttgt gaaaaagctg ctctgcatca agcagtaaga acaatcagat aaaaatagca
     2461 ctccatattt ttcatctgat tgggcttagt gccgtagatg caagcttttg aacaccgttt
     2521 gttcggaaaa agggactgtg acacggtttt tgtcacagtc ccttattctg aataaacagt
     2581 gttcaagaga cagcctatcg gcaataaggc taaacgtctc tttcactacc tcttcaagtt
     2641 attattatcg tatagataaa cacttcgact cgctctggta agaacgtcct gattcagtcg
     2701 tcgtttttat ccttagattt gcgcccagac gctttctaag atgtttgttt gtgttctatc
     2761 aggtccgaca gaaaatgttg agatacgtac accaacaagc tctgagatac gacgtacata
     2821 gttacgtgcg ttttctggca gttctgcaag tgttttgcat cctgtaatgt cttctgacca
     2881 gcctggcaat tcttcataga ttggtttgca gcggttcaac tctttcaagc ttgctggata
     2941 gtggtagatt agttcgccat ctaattcata agccgtacaa atttttactg tttccaatcc
     3001 acttaataca tcgatcgagt ttaaagatag gttggtaata cctgaaactc gtttagaatg
     3061 gcgcataact acagaatcaa accagcctac acgacgcgga cgaccagttg tcgtaccgta
     3121 ttctcgtcct acttcgcgga tttgttctcc tgtttcatca aataattcag ttggaaatgg
     3181 tccatcaccg acacgtgacg tataggcttt gcagacgccg acaactttat tgatttttga
     3241 aggccctaca ccgcttccga ttgttacccc accggctact gggttagaag aagtaacaaa
     3301 aggataagtt ccctggtcga tatccagcat tacaccttgt gcgccttcaa acaacacgcg
     3361 tttgcctgca tccaaagcat cattcaagat aacggaagta tctgtgacgt attgtttgat
     3421 ttgttgtccg tattcataat attcttcaaa aatatcgtca aaatcaagtg gttctgaatc
     3481 aaacattttt acaaattgac ggtttttgtc atctagattc aaacgaagac gttcttcaaa
     3541 aatttcttta tctaataagt ctgcaatacg gatacctaca cgagctgctt tatccatata
     3601 ggcaggtccg attcctttaa tcgttgtacc aatcttattt tcgcccttag catcttcttg
     3661 taattggtcc aatttgatat gataaggcaa gattacgtgc gcgcggtcag aaatacgcaa
     3721 attacttgtt tctacacctt tttcatgtaa ataagctaat tctttaacca atgatttagg
     3781 attgactaca acgccgtttc cgatcacact gatcttgtct ttataaaaaa tcccagaagg
     3841 aatcaaatgc agtttataag tcacaccgtt aaacttaatt gtgtgccctg cattgtcgcc
     3901 accttggtac cgtgcaatca cttcagcatt ttcactgaga aagtctgtga tttttccttt
     3961 cccttcatcg ccccattgcg tacctactac tactacagat gacatgtgaa cacctcgttc
     4021 tatattattt aatcctgaac cattgtatca ataatagata catatttcaa ataaaatcga
     4081 atgattttta tttttatttt ataaaaacgc ttaaaaaacg aactttatct ttttagaatg
     4141 gttattaaaa aagaggttgt aacagaagtg gtcagcttca agaaataaga aggaatgtac
     4201 gaaaattgct tctcaaattt ttgtgaaatt tcgacttatt tttcgaagaa gctacttttg
     4261 gaacaccgtg aatcagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
     4321 aacaaaaata gcttctcata tttttcgttt gattgggcgt attaccgcag atgcaagctt
     4381 ttgaacaccg tttaagaaag aggttgtgag cctgccgttt aactctgagt cacaaggagc
     4441 aatttcaaaa aactgcttct catgtttgat gatattgtga cttgtgaccg aggagttggc
     4501 aggagaacac cgtttaagga aaagaggtcg taagatcagc gttttgacct gagtattaga
     4561 gaaacaaacg tcaaaatagt tcctcatatt ttgacgtttg ctgagctaat accaagggtc
     4621 agctgattga acaccgttta ttcggaaaaa gggactgtga caagactttt gtcacaatcc
     4681 ctttggagat ctgtttaaat taagtactct agtcgttcct gcggttactc cttgcatctt
     4741 cttacgagag ctctcgggat tgctcaaggc tcacaacctc ttctttaaaa ttcttctctt
     4801 ggcgaaagcg acgaaaactt attgtattct ttgataaaca gtagttcaac tgttcctcgt
     4861 gcaccgcttc ggttcttctc gatgatgact tcgatgacgt tgtcattttt tggctcgttt
     4921 ccatcatcgt catctccgcc ttctctttca taataatcat ctcgatacaa gaaggctacg
     4981 atgtccgcat cttgttcaat tgatccggat tcacgaatat cacttagcac tggtcgtttg
     5041 tcttgtcgct gttctactcc acgagataac tgtgaaagag caatgacagg tactttcagc
     5101 tctttcgcca gttttttcag ttggcgagag atatctgata cttcttgttg acggttctct
     5161 ttaccgttac cttcaatcaa ctgcaaataa tcgatcaata tcaagcctag attgcctttt
     5221 tcttgcgcta gtttccgaca tttcgcacgg atttcagaga ttttgatccc tggtgtatca
     5281 tcgataaaaa tatttgctcg agaaagactc cccatcgcaa cgatcagatt cgaccattct
     5341 tcttccgaaa gctgacctgt acgtaaatgg ctggcctcaa tcgaaccttc tgcacaaagc
     5401 atccgattta caagagattc tgcccccatt tccaaactaa atatcgcaac agaacgatct
     5461 gtttttgttc ctacattttg agcaatattc aatgcaaacg ctgttttacc taccgctgga
     5521 cgtgcagcaa gaatgattag ttcttcgggc tggaaacctg ctgtcatttt atccagacct
     5581 tgatagccag ttggtagacc tgtgatttct tcattgtttt gagctagtcg atcgatattt
     5641 tcaatcgtgg agttcagtac ttcagcaata gataaaaagc cgcttcgatt tcttttttcc
     5701 gaaacctcca agatactgcg ttcggcatca tctaaaattg tttgaacttc ttctccttgc
     5761 tcgaatcctt tcgtaacgat tcctgttgct gtctggatca atgtacgcag caatgatttt
     5821 tcttcaacga ttttcgcata gtagacgata tttgctgctg tcggaactgc taatgccaag
     5881 tctgagagat aacttaaacc accggcatct tctaacaaat tggcttgttc cagccggtct
     5941 ttgacagtaa ccacgtcaat cgcttcatta cgatcattta actcgatcat tgtctcaaaa
     6001 ataatttgat ggccacgacg ataaaagtct tttggctcta tgtactccat ggcatcaatg
     6061 atcgtttctg cgtcaagaaa aacagagcct aaaaccgctt gttcagcttc aatattttgc
     6121 ggtgggattc gatcctgcca cacttcattc atacgtatcg ctccttgtct tcaattgctt
     6181 cattttacaa gaaaatgaga gaaaagtcat cattcgtacc gaaagtgtaa taaaaaaata
     6241 gttctctttt attatactcg ttttctttat gctattttgc tgttcccaaa aaagaaaccg
     6301 aaatcaatct gaaatcgatt tgcagctttt gctttcattg ctgcagctgt tttctcagat
     6361 aaatttcggt ttgtcttttt tccttctggt tatacttacc agctgaacat cgctatcaag
     6421 atttgttatt cttgtacgac atgaactttg atcgtagccg tcacctcatg atgaagctta
     6481 acaggtacgt ttgtgtagcc tagtgaacgg atcggatttt ctaattcgat ttttcgttta
     6541 tctaatttga ttttgtattg tttgtttaac ccttcagcaa tttgttttga agggatcgaa
     6601 ccaaacaaac ggctgtcttc ccctgctttc gccttcaatt cgactacagt atcttctttt
     6661 tccaagaatt cttttaactt tttagcttct tccaagattt cagcttcgtg tttttcttgt
     6721 gctttctttt gtcctgctaa ctgactgata ctgcttttcg ttgcttcttt tgccaagtta
     6781 tttttgatta gataattttg tgcataacct gctggaactt ctttgatgtc ccctttttta
     6841 ccttttcctt ttacatcttg taaaaaaatt actttcattt ggattactcc ttatcatata
     6901 tttgattcat attttctttg attgcagcaa atagcttctg ttttgtttcc tcaactgtcg
     6961 tattttccag ttgagtagct gcgtttgtga agtgtcctcc gccacctaag gattccatga
     7021 tgatttggac gttgatcgtg ccggtacttc tggcactgat cccaatcaag ccgtctgtcc
     7081 gctttgtaat gacgaaagct gcattgatat tgatcatcga aagcaatgta tctgctgttt
     7141 ttgctgctat aacactatta tactcttttt cgtctgtccc tgccgccaca acaatatcag
     7201 aagtcacata gtcgcttcca gcaatcaaat tgctgatctc caagtatgtc gctaaatccg
     7261 agctcaataa atattgtact aaggatgcgt ctgctccaca agaccttaaa tagctggcaa
     7321 cgtcaaaagt tcgtgcagtt gtacggacat tgaaactatt tgtatctaca accatccctg
     7381 caagcaataa tgtggcctca aaacgatcta agcgatttgt tttgctgctt tggtattgga
     7441 tcagctcaga aactagctct gaagctgaag aagctgaaga ttcgatataa gtcagcagag
     7501 gtttacttgg aaactcttcc ccacgacgat ggtgatcaat gatcacaatc ttatcaaatt
     7561 gctcatatag gccctgagag atcgatagag aaggtttatg gtaatccacc atcacgagga
     7621 ggtcctcttc ttttttcagc tctatggcac gttctggaga gattagttgc atttctaatt
     7681 caggatgctt atggatttct tcaagacacc gttctacatc tggaatgatc tcgttctcgt
     7741 tgataacgat gtaagctttt ttctggttga agcgtgccag acaactgaca ccaaaagcag
     7801 agcctaaagc atccatatcc ggaaacttgt gccccatgat aaacacatcg cctgtttcat
     7861 caaaaatccg acgcaaagcg gtactcatcg ctcgagaacg aacacgcgtg cgtttgacat
     7921 tcttagcaga tttcccccca taataaacag gttttgctcc ttcttttacc tctttcacga
     7981 caacttggtc gcctccacgg actaaagcaa catctaaatt attctgagct tctccaccga
     8041 tttcatcgag ggttcttgag ccataagcaa tccccatact aagggtgata ggcgagctct
     8101 gtttttcagc ttctgctcga aagcgatcca gcaaatcaaa ttttttatcc atcatctttt
     8161 tcagatcgat catctgtgct acgaagaaaa accgttctgc gttgattctt ttgaaaaaga
     8221 catggtattc attcatccag tcagaaacta gtgtagtgac gaaagagttc aaataagaaa
     8281 tctctttgtc atccaattta tcaatggcat ctgcatagtt atcaatagat aaaataccaa
     8341 tcaccggctg actgttcgtc tgatggagtt ttaacaactg gttttgggta atatcattaa
     8401 aataaacaac accctttact gtatccatat caaactgaaa tgttttatct gctagataga
     8461 cttggttttt ctttttttct tttgcagtaa aaaaagtatc tagcaattct tcctctgtta
     8521 atccactttg atctttgatt ttcaatgcta atggattgag ccattcgatt ttttggcttg
     8581 atggctgata agcaatcact cctacaggca tgacttctga gacataagct agattggttt
     8641 cagctacagt cgaagcctgc tggatttttt cacgtttatt taaaataagc caacgtttca
     8701 ttttcaacca atagcgaaca ccccataaat tcatcaatag aatgaccgaa gcgctaacaa
     8761 aacgaaatgg taaaattaag actaccaata cttcgattaa tataaacagg ataaggaaga
     8821 gaggtttcgg ggataaatga ttgtttttat tttccataat tttcctccca tatatgacta
     8881 atttgatttt accataactt tcgtcattcg agcgactaaa cgaatagagg ttatttttac
     8941 agaaaaaaag gaattgaata gctgatcaaa aggatcagtc attcaattcc taggatttat
     9001 tagtcttcgc taacgaaagg tagcaagccc atgatacgag cgcgtttgat tgcaacagta
     9061 attttacgtt ggtttttagc gcctgtacca gttacacgac gtggcaagat tttaccgcgt
     9121 tctgaaataa atctttttag taattcgata tctttataat cgatatattc aatatggtta
     9181 gcagcaatgt agtctacttt acgacgttta cgtccgcctc ttctttgttg tgccattcta
     9241 tttccctcct atcaaattcg tttagaatgg taaatcatcg tctgaaatgt cgatcgttga
     9301 acttgagccg aatgggtcag aagtatcacg gtcgaaatca ggcataccgt tggatgattg
     9361 agatgtttga ttagagttat tgtttccgcc aaatccgcca gctgattgac cagaactttg
     9421 gaatccgccg ctttgttggc gattttcaga tgcagaacga ctttccagca attggaagtt
     9481 gtcagctact acttctgtca cgtatacacg ttgtccttgc tgattatcat aagaacgagt
     9541 ttggatacgt ccagtaacac ctaaaagtgt tcctttacga gcatagtttg ctaaagtttc
     9601 tgctgatttg cgccaaatca cacagttgat aaaatcagcc tcgcgattgc cgctttggtt
     9661 tgtaaaatta cgattcacag ccaaagtaaa tgttgcgact gccgtaccac ttgctgtgta
     9721 acgcaaatct ggatctttcg tcagacgtcc aactaataca acattattaa tcaaaatagt
     9781 catctcctct catcgttttc tattgtttca catgaaacaa caattaagct tcttctttaa
     9841 caatcatgtg acgaatgata tcgtcgttga ttttagcaag acggtcaaat tcattgacag
     9901 catttgctga tgatggagaa gatactttaa cgatgtgata gataccttca cggtaaccgt
     9961 tcatttcata agcgaaacgg cgtttttccc aatcttttga ttcgataact tctgctccgt
    10021 tatctttcaa gattgcatcg aaacgttcga ttaaagcagt ttttgcttct tcatcaatgt
    10081 ttggacgaat aatgtacatg atttcatatt tcgtgttttc cattgatatt cacctccctg
    10141 tggactttcg gctcttaggt tcctaagagc aaggagagtt atctaattaa ttagactact
    10201 cacaagctct gattatacac tctattttgg tcaaaagcaa tacagaatga acagtttcga
    10261 atattttaag agaaattcaa ctaatcaaaa aagccagcag gaataaccag attttttaat
    10321 cggtcattct tgctagctta cacaattagt cttgatggct attcttctga tccttgttct
    10381 tcaactgttt gttctgcagc ttctggttcg actgctgcca tcgtgacaac tttcgtatca
    10441 gcttccatct tcatcaaacg tacgcctaat gttgaacgtc cagtttgaga aacagtcgat
    10501 acgttgaaac ggataatgac acctttatct gtaatcagca tgatgtcttc atctccagaa
    10561 acagtggtca atccagctag tggaccattt ttttcagtga tgttcgccgt tttgatccct
    10621 ttacctccgc ggcctttcac tggatattcc gatacttttg tccgttttcc gtaacctttt
    10681 tctgtgatca ctaatacttc ttgatcttcc ttcattaagg ctgccccaac gacataatct
    10741 tcctcacgca ggcgtattcc tctaaccccg gaagctgtac gtcccatatc gcggactgct
    10801 tcttctttga aagtgacaga gtagccgtta tgtgttccaa taatgatatt agattgacca
    10861 tctgtcagta gcacattgac gagctcatcg tcttctttca aaccaattgc gatcagacca
    10921 ttacttcgga tgttagcaaa agcagtgaca gaagtacgtt ttactgtacc ttgacgcgtc
    10981 gtaaagaaga gatagtggcc ttcttctgct tctcccgtca ccgcaatgat tgcctggatg
    11041 ctttcactcg aatcgatacc tagtaaattg ataattggta ttcctttggc tgtgcgcccg
    11101 tattctggaa tttcgtatcc ttttgcccga tacacttttc cgttattcgt aaagaataac
    11161 aatgtatcat gggtagaaca agaaaccaga tttttgacga agtcgtcatt gtggataccc
    11221 attccttgga caccacgtcc accccttcgt tgagctctga attcattatt tgccaaacgt
    11281 ttgatgtaac cattgtttgt caatgtaata acgatttctt cttcttcgat taaatcttca
    11341 tcttccagac ttaagacctc accgactagt aattctgtgc gtcgtttatc accaaatcgt
    11401 tgattcaagt ctgctaattc attcttgatg atttctgtaa cacgttctgg tcttgccaga
    11461 atgtcagtta aatcttcaat cagttttaag agttcttggt attcattttc gattttttcc
    11521 cgttccaaac cggtcaatcg gcgtaaacgc atatctaaaa tagcttgaga ctggcgatcg
    11581 gataaatcga accgttcgat catcgtcgct tttgcttcgt catctgattt agacccgcgg
    11641 ataatcgcaa tgatttcatc gatatgatcc aaagcaatac gcaaaccttc taaaatgtgt
    11701 gcccttgctt ctgctttttc tttttcaaag actgtccgtc ttgtgatgac ttcgcgctga
    11761 tgttcgatat agttttcaag aatttgtttc aagctcaaaa ctttcggcac acctttttcg
    11821 atcgccaaca tattgaatcc aaatgaattt tgcaaggatg tcaatttata taagttattc
    11881 aagataacag atgcgctggc gtcacgacgg atgtcaatga cgatccgcat accttcacgg
    11941 gaagattcgt cccgcaaatc tgtgatccct tcgatacgct tgtctctgtg cagttcagaa
    12001 atccgttcga tcaattttgc tttattgacc atataaggca attctgtcac gatgatccgt
    12061 tctttaccat ttggcatctc agtaatctca acttttgcac gtacggtaat agaaccctta
    12121 cctgtttcat acgctcttcg gattcctgat tttcccataa ccaaaccacc tgttggaaaa
    12181 tctggtccag gtaatacttc catcaattca ttggtcgtca catcaggatt atccatcagc
    12241 aaatcgatag ccgctactac ctcagacaag ttatgaggtg ggatattcgt ggccatacct
    12301 acggcgatcc ccgttgttcc gttcactaaa agattcggga agcgagcagg tagtacaact
    12361 ggttcttttt ctgtatcatc atagttgctt tggaaatcaa cagtgttctt attgatatca
    12421 cgcagcattt ctgtagcgat tttgctcatc cgtgcttcgg tataacgcat agcggcggca
    12481 ccgtcaccgt caacagaacc aaagtttcca tgaccatcca ctagcatata acggtagctg
    12541 aatggctgtg ccatccgtac cattgactca tagatcgcat agtctccatg aggatggtat
    12601 ttacccatga catctccgac gattctggca gattttttat gggctttatc cggtgttaca
    12661 cctaattcgt tcatgccgta taaaatacgg cgatgaacgg gtttcagacc atctctgaca
    12721 tcaggaagtg cacgtgcgac aataacactc atcgcataat cgatgaagga gtctttcatt
    12781 tcgctggtta gattgacatc atggatgttt tctctgattt cttcactcat tctttttcct
    12841 ccttcatgat taaatatcta agttcttcac atagtgtgca ttttcctcga tgaatgcacg
    12901 acggggttct actcgatccc ccatcaacat ttcaaatatc tgatctgctg caatagcatc
    12961 gtccacactg acgcgggcca tcatccgatt gcttgggtcc atagttgttt cccatagttg
    13021 atgatcatcc atttctccaa gtcctttata acgctgaacg cttggttttg gtgaggcagg
    13081 aagtgaagca acgacttgtt tcaactcttc ttctgcattt tttcctggtt gtacatacgt
    13141 aatattcttc ccttgtttta ccccgtataa aggtggctga gcaatataga cgtatccagc
    13201 ttcaacaata ggacgcatgt aacgataaaa aagagtcagc agcaatgtac ggatatgcgc
    13261 gccgtcgaca tcggcatcag tcatgataac taatttgtgg tagcgagctt tcgatacatc
    13321 aaaatcttct ccaaaccctg tacccatagc agtaaataaa gaccggatct cttcatttgc
    13381 caagatcttg tccatgcttg ctttttcaac attcaagatt ttcccacgaa taggcaagat
    13441 ggcttgaaac tctcgattac gaccttgctt agctgatccg ccggccgaat ctccttcgac
    13501 gatgaacaat tcgcattttt caggatcgtt gctagagcag tccgctaatt ttcctggcag
    13561 attactaatc tctaaagcac ctttacgtct cgttacttca cgcgcacgtt tcgctgctag
    13621 acgagctctg gaagctagta atcctttttc aacaatttga cggcccacac ttgggttttc
    13681 cagcaagaat ttcatgaaat gttcgctaaa taaccgatca gtgactgtcc gtacttcgga
    13741 attgcccaat tttgtttttg tttgtccttc gaattgaggg tcaggatgct tgatagaaat
    13801 aacagctgtc aatccttctc gcacgtcttc accggtcaag ttgtcgtcat tttctttcat
    13861 cagcttttgt tttcgggcat aatcatttat cacacgagtt aaagcagtct tgaatcctga
    13921 ttcatgtgtt ccgccttcat acgtatggat attgttggca aaactcatga tattggaatg
    13981 gtagccgtct gtatattgca tagagacttc aactgcgata tcttgctgtt caccttccaa
    14041 atatacaggt tctggaaaaa tcacggtttt gtttgcattc aagtattcga cataactttt
    14101 aatcccgccg tcataatggt attcattgat gactggttct tcttctcgtt tgtcttcaat
    14161 cgatatacgc atcccgcggt tcaagaaagc aagttcacgg atccgggtag ccaattttga
    14221 aaaatcaaac tcagttgtct ctgtaaaaat ttcagggtct ggcgtaaagt gaaccgttgt
    14281 tccatggcga tccgtttgtt cgataatctt taagtcgtcc actacagctc cacgacgata
    14341 ttcttgatag tacacatttc cttctttgta tactttgaca tcaagctgtg tagaaagcgc
    14401 gttaacaaca gaagatccta ctccgtgaag tccaccagaa actttgtacc caccaccgcc
    14461 gaattttcct ccagcatgta aaacagtaaa gaccgtttca actgcaggac gtccagtttt
    14521 tgcctggata tctacaggta tcccccgacc gtcgtcaata acggtgatac tgttgtcttt
    14581 ttcaataatc acatggattt tcgttgcaaa acctgctaat acttcatcga tcgaattatc
    14641 gacaatttcc caaacaaggt gatgcagacc ctcagaacta gtcgaaccga tatacatgcc
    14701 aggtcgttta cgaacggctt caagtccttc aagtacttga atctgactgg catcatactc
    14761 tttagcgcgt tctactaaac ttctttcttc tgtcattcag tttcttctcc ttttagtttc
    14821 ccttgttgaa cataaaaaat ctcaggttca acagtcattt tattttttac atggtcaagt
    14881 gtcgttgttg ttaagaacgt ttgaactttt ccttcgattg tttctagcag atggagttga
    14941 cgactatcat ccagctcgct catcacatca tctaacaaaa ggattggata ttcccccgtc
    15001 tcttctttaa tcaaatcgat ttctgctaat ttgacactta gtgcagttgt tctttgctgt
    15061 ccttgcgatc cataagtctg cacatttttc tgattaataa aaaaggaaag atcgtcacga
    15121 tgaggaccaa ctgtcgttgt tccccgaaaa aggtctttct ttttattttg gttcaaaagt
    15181 gcgagaaatt gttcttggat ttgctcttgc gtatgtgttt ctaaagaatc gactgcggtg
    15241 agatactcga tttccagctg ctctttttga tgggtgattt gctgatgcaa agaattagcc
    15301 caaaactcca atcgcttgac gaactgtgct cttgctttca atactttgct gccaaacgct
    15361 gctagttgtt cggacaaaat atccaaataa agcaaatcag tttg
//
LOCUS       DAYLZO010000069        15230 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.070, whole genome shotgun sequence.
ACCESSION   DAYLZO010000069 DAYLZO010000000
VERSION     DAYLZO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.070"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            278..787
                     /locus_tag="ACGVJG_001934"
     CDS             278..787
                     /locus_tag="ACGVJG_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFJ6557863.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACGVJG_001935"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACGVJG_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFJ6557864.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACGVJG_001936"
     CDS             2322..3302
                     /locus_tag="ACGVJG_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6557865.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACGVJG_001937"
     CDS             3424..4842
                     /locus_tag="ACGVJG_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFJ6557866.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACGVJG_001938"
     CDS             4913..6382
                     /locus_tag="ACGVJG_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFJ6557867.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACGVJG_001939"
     CDS             6392..6859
                     /locus_tag="ACGVJG_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFJ6557868.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACGVJG_001940"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACGVJG_001940"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFJ6557869.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="ACGVJG_001941"
     CDS             7848..10382
                     /locus_tag="ACGVJG_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFJ6557870.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="ACGVJG_001942"
     CDS             10538..11311
                     /locus_tag="ACGVJG_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFJ6557871.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="ACGVJG_001943"
     CDS             11304..12095
                     /locus_tag="ACGVJG_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFJ6557872.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="ACGVJG_001944"
     CDS             12105..12596
                     /locus_tag="ACGVJG_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFJ6557873.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="ACGVJG_001945"
     CDS             12614..13024
                     /locus_tag="ACGVJG_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFJ6557874.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="ACGVJG_001946"
     CDS             13028..14428
                     /locus_tag="ACGVJG_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFJ6557875.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="ACGVJG_001947"
     CDS             14451..14825
                     /locus_tag="ACGVJG_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFJ6557876.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2134 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DAYLZO010000070        15013 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.071, whole genome shotgun sequence.
ACCESSION   DAYLZO010000070 DAYLZO010000000
VERSION     DAYLZO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.071"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            42..914
                     /locus_tag="ACGVJG_001948"
     CDS             42..914
                     /locus_tag="ACGVJG_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFJ6557877.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            1090..1452
                     /locus_tag="ACGVJG_001949"
     CDS             1090..1452
                     /locus_tag="ACGVJG_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFJ6557878.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            1461..2213
                     /locus_tag="ACGVJG_001950"
     CDS             1461..2213
                     /locus_tag="ACGVJG_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557879.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            2227..4029
                     /locus_tag="ACGVJG_001951"
     CDS             2227..4029
                     /locus_tag="ACGVJG_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6557880.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            4306..5346
                     /locus_tag="ACGVJG_001952"
     CDS             4306..5346
                     /locus_tag="ACGVJG_001952"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFJ6557881.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(5398..7542)
                     /locus_tag="ACGVJG_001953"
     CDS             complement(5398..7542)
                     /locus_tag="ACGVJG_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFJ6557882.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(7899..9272)
                     /locus_tag="ACGVJG_001954"
     CDS             complement(7899..9272)
                     /locus_tag="ACGVJG_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFJ6557883.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(9272..9667)
                     /gene="rbsD"
                     /locus_tag="ACGVJG_001955"
     CDS             complement(9272..9667)
                     /gene="rbsD"
                     /locus_tag="ACGVJG_001955"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFJ6557884.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(9695..10612)
                     /gene="rbsK"
                     /locus_tag="ACGVJG_001956"
     CDS             complement(9695..10612)
                     /gene="rbsK"
                     /locus_tag="ACGVJG_001956"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFJ6557885.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(10720..11733)
                     /locus_tag="ACGVJG_001957"
     CDS             complement(10720..11733)
                     /locus_tag="ACGVJG_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFJ6557886.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(11814..12413)
                     /locus_tag="ACGVJG_001958"
     CDS             complement(11814..12413)
                     /locus_tag="ACGVJG_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFJ6557887.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            12599..13300
                     /locus_tag="ACGVJG_001959"
     CDS             12599..13300
                     /locus_tag="ACGVJG_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557888.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            13310..>15013
                     /locus_tag="ACGVJG_001960"
     CDS             13310..>15013
                     /locus_tag="ACGVJG_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFJ6557889.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SS"
BASE COUNT         4606 a         2775 c         3075 g         4557 t
ORIGIN      
        1 attagatatt tattgaagga taaaggagcg caggaagtag catgagagaa aaaagagcat
       61 tttatatcaa tggttatgca gggattgtcg gattggtcgt attagcttta gttggattgt
      121 ttttcttcta tttaggtatg tggcaagcga aagtttgggc acttttttta agtatttttc
      181 tttggttgat cacgttattg ttgttaagtt cggcaactgt agtcagtcct aatcaagcaa
      241 aagtaatttt gttttttgga caatatttag gtacgattcg agaaaatggc tttttcctta
      301 cgataccttt ggcacaaaaa atgactgttt cgttgaaagt tcggaatttc aatagttctg
      361 ttttaaaagt caatgatttg gatggaaacc ccatcgaaat ctcagcagta gtagtcttta
      421 aagtcgttga tacagcaaag gcattattcg atgtagcgta ttatcaagat tttgtagaaa
      481 tccaaagtga gacagccatt cggcatatcg ctagccaata cccgtatgat acattcaatg
      541 agaatgattt gacgcttcga ggaaacacga cggcagtgtc tgacgagttg caaaaagagc
      601 tgcaagagcg tttagcagta gcaggtgtcg aagtaatcga aacacgcttg aaccatttag
      661 cctatgcaac agaaatcgct agtgccatgc tccaacgtca acaagcaaaa gccattttgt
      721 ctgctcgaca aaccattgtt gaaggagctg tcacaatcac tcaaatggcc ttagagcaaa
      781 tcgaagatgg acaagaaatc aatttcaccg atgatcggaa agtccaattg atcaataatt
      841 tacttgtctc aatcattaca gacaaaggaa cgcaacctgt tatcaataca ggcgacatga
      901 aagaccgtaa gtgacatact ttttgtaaga taggaagaag gagtttagga cggattattg
      961 tcttgaactc tttttttagt tgttttaaag aaaatgacaa aattgaaaga agaatgatag
     1021 ttgattcgaa ggcagaattg ttcgcttttt tctataatca gtatataaag taagagagaa
     1081 ggggaaaaag tgaaaaaact tctgatcggt ctacttgttt tcatcgttgt ggtacttgca
     1141 ggggtgaaaa tcgcagatta cgtagttatg ggtggagaag aatactatgt acaaattacg
     1201 acagatggta caaaaacagt ttcaaaatca gactctggac aagaaacagt caactataga
     1261 tatatcctgc ctggctatac gaaagagggt aaggaaaaag aactggactt caagggacaa
     1321 ttgactcgac cgcttcgtaa aggagcatat ttaaaaatca cctggaacaa aaataaaggt
     1381 gtcacttcgt atgaagaagt cgaaaaaaaa gacgtgccga aaacagcact ggaaaaacta
     1441 attaaagggt agggaaagaa atgaacaaaa tagtcgaagt acagaatgta acaaaagtat
     1501 acggaaaaaa caatgagaaa aagacgcaag cattaagcgg catcagcttc gatgtagaaa
     1561 aaggcgaatt tattgggatc atgggcgctt ctggttctgg taagtcgaca cttttgaata
     1621 ttttatccac attagataaa ccaacagatg ggcatatccg aatcaatcaa aaagatgtga
     1681 ccacattaaa aggaaatcaa ctagcagatt ttcgtgcgaa tgagattggc tttattttcc
     1741 aagatttcaa tttattagaa agcttgacag cacaagaaaa tatcgctgtt cctctttctt
     1801 tacaaggcgt cagaccaaaa gaaatcaagc aacgtgtcca aaaaattgca gaacgtttat
     1861 ccatctctca tattttggaa agctatcctt ctgagatttc cggtggacaa aaacaacggg
     1921 tcgccgccgc acgtgcattg atcacacaac caacgattct attaggggat gagcctacag
     1981 gtgcgctgga ttccaaaagt gcccgggatc ttttagatac gatggacgaa ttgaatacaa
     2041 aagatcatgt atcgattctt cttgtgacgc atgatccgtt ttctgcaagt tattgccaac
     2101 gtattttatt catcaaggat ggcggtatcc atcaagaagt gaaacgtgga gatcaaagcc
     2161 gtgattcctt ctatcgagaa atactatcta ttcttggaaa cttggaacag tagggggaga
     2221 ttgactatgc tttggaaatt atcacttact gggatcaaga gccgtttaag agactacttg
     2281 gtgttattct caggattagt tatggcatct gctatttttt acatgtttga atcaatggca
     2341 agcaacgaag cttttttgaa aagtaatacg atcgttgcgg ctacagttat cattttccga
     2401 ttcggttctg ttttactagg gatcatcaca ttcgtttata ttttatatgc aaattccttt
     2461 ttgatgacga tgcggcaaaa agactatgca atgtttatga tgctgggagc aaaaggcaga
     2521 aaaatcgcac agatgatttt ttctgaaaca ttcttagtag ggatcgcagc tactttagtg
     2581 ggttcagctg tcggtgtagg attgacaagt atcgtccacc gattattagt ggatcagtta
     2641 gatctcaata tttcgcattt tacacctttt agtattcacg ggttgttgat cactgttttg
     2701 ttttttgctg ttttgttttt attagctgct gttgtcaatg ctttttcgat tgtcaaaaaa
     2761 ccgattttag cattgattcg tgcggatcag acaccaacaa gaatcaagca gcataaattc
     2821 ttgtttttcc ttgaagtagt actgggaatt gcttttctag gctgcggtta ctatatgatg
     2881 aaacacgtgg ggacctatca agtctttggt ataggggtag ccttagttac gatcgtatta
     2941 ggaacttatt tcattttcca ttctgtgatc attttctttt tatcattact aaagaaatca
     3001 gaacagattt cattgaaaaa attgaacaat ttcacattgt cacagttaaa ctttcgtatt
     3061 cgtgaatata cacaaatgct ctcaatggtc gctatgctgt ttgccttggc actgggcgct
     3121 ttgacggtag gattaggttt taaaaatgaa attgcagact tgacagaaaa caattctgga
     3181 tatgatctag tgatgaataa tcctcgggca gaagatcagc aaaaaatcaa agaactttca
     3241 ccaacacttt ctgcttctta tactcaaaaa gaagatgcgg gaacgattta ctataatgca
     3301 agtgaatttg cggctcaacc attgatcatg ataaaacatg aagatggaac accgccgaag
     3361 atcacgaaag aaaaagttcc tgctaaaaaa atgaatgaac tgactgtaca agaggaactt
     3421 cgaagctatg aattgccgga acagcaagaa aaagaaatca aggtagtcag tcaatcagaa
     3481 tttgatcaac tgaacttgcc acaatccact ttacagctag ttcaggtaaa agacttccaa
     3541 gcaaacctga aaccaatcga aacacttgtt gctcaaaata aaatgaacaa tccatctttg
     3601 caaaaagtag agtacagcaa ccagaaatat gaaatgtatg aagtgtataa tggtctattc
     3661 tctggttttg aatttatggg attcttccta ggtttagctt tcttgaccat gcttgctagc
     3721 tgtttgatgt tcaagatctt gtcaggaagc aagagtgaca ttgctcgtta tgaaatgctt
     3781 gaaaagatag gaacacgacg cgggttgctt aaacaatcga tccgtagaga agtaggcgtg
     3841 ttattcttag caccaggaat cttaggtgta gtccatgtct tattcggatt acaaatgttt
     3901 ggtctattca tgcaaaatcc atacaaagat atttgggttc cgtttacgat attcttcgta
     3961 ttgtatttca tctactatgt actgacgaca tggttatata ctggaatcgt attgaaaaag
     4021 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa aatagctcct
     4081 catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt
     4141 cggaaaaaga ggttgtgaca atatttttgt cacaaccttt ttttcacggt cttttattag
     4201 aatatagtaa attttttctc gttactcacg gaataagctg ttaagaaaac ggtttccatg
     4261 tatactggaa tcagtgcaaa tgaaagaaaa gagggtgcaa ggaacatgga aatcaatgaa
     4321 caaaattttg gcgaaaacta tcacttgatt acgatcacga atcaaaatgg gacatctttg
     4381 tctgtcagtg atcttggggc acgggttgtt cattttcaaa caataatcga taatgaaaaa
     4441 cgagagttga tcttaggttt tgatgaagca aaagaatatc tagaaaaaga cccttatatc
     4501 ggtgcttcaa tcggacgtat ggctggacgg attacgaatg gcacattcaa attagatgat
     4561 gaaacgttcg aagtaaaaac agacccggac actggtcatt gtttacatgg tggtgcacct
     4621 agctttgaag ccaagcagtg gcattataca gtcatcaatg gtgaaaacga agcatcggtc
     4681 attttctatc agactagccc agatatggaa aacggatttc ctggaacttt agatgtagaa
     4741 gtacggtata cgctgacgaa cgatgataca tggcgagtga cggtccaagg gatcagtgac
     4801 cgcaaaacag tattcaatcc tacaaatcat gtctatttca atttaaccgg tgatccagca
     4861 cagtcaatcg acgaacatat actttggttg aatagccgat tatttgctac gctcaataaa
     4921 gatacaacgc ctacaggaga agtagtttca gtaagtggca cagcatttga cttccaacgt
     4981 cctaaaaaat taggtgaggt ctttgccagt gattttcgcc aatcaatgat agtgggcggc
     5041 atcgatcatc catttttgtt gaaaaaacaa tctggtttac taaatgctgc ggaattgaca
     5101 agcccagatg aaaaagtatc gatccaagta aaaacagatg cttcaggaat cgtgattttt
     5161 acagcaaatt ttggagaaga tggaccggaa atgcgcggga ataaattagt aaatcatggc
     5221 ggtatcacat ttgagactca agaactgcca ggagcggagc gacttgaaag ttttggagat
     5281 gtcactctgg aacctaacca agtacgcgag tttgtaacgg aatacaaaat aaatataaag
     5341 aaatagaata aattaaaaag ggagtgtgac agtgtcacat ccccttttta atttattcta
     5401 tttctttaaa ggcagatcaa gccagaatga tttgtctaaa cttaacaagg ttagtacagg
     5461 aacagttgca gctttaactg tacgagtagt tgtttctggt atctgtcttg ccgtaaaatt
     5521 gttccacaaa tcccagattg aagcgacacg gaagatggaa gaagcgttgt tgacgcgttt
     5581 tatcgttgaa aattctaatc ctgtgattgg cacgccatct gttgtccatt tgattggcac
     5641 atcgaatcgc ttagacatat tgaggatttc agcgtttgct tcttcagttg ttgctgcttc
     5701 ttttttcggt tcatcatatt ttgtcaggtc gtacgcttcg atgatcgcgt tcaatttttg
     5761 tgaatattgg cgatctgtag cataacgtcc ttccaaatat ttggttgctt ctttgtagtc
     5821 ttttgtttcc gacttccaag ttggtttgta gaaatcagga ttgccagtca gtccattttt
     5881 gatcaagtcg gcatagtctt ccaaactttc cttgtaagaa ggatatttac ggaattgtgc
     5941 gcggatttgg tagctttgtc cggaactatc ttgttcaagt gtattgaagg tcacgctgct
     6001 tcctttatat gatcctttga taccaaaaag gttatgattt ggttcgcttg ataataagct
     6061 gtttccagat ccactttcaa ggatagcttg agccatcatg actgatgcgt agattccttc
     6121 tttttgcccg atttcctgtg catcatctgc aatctttttc aagaactgat cagttgtttg
     6181 gttttgactg aaatggaacg agccatctaa tccatctaaa atagatgagc cgcctgtcag
     6241 cgaacctgaa gcagcaggta aacctgctaa gtttactcgg acagcaaaat caggcgtgaa
     6301 atctgaaata gaagttactc gaactttatc tcctggttga ggcgcatgga taaattgtcc
     6361 gttcccaata tagataccta catggtgagt aacaccggaa gaaggtcccc agaagacgag
     6421 gtcgccaggt tgggcctgac ttactggaat ctgcgtacct gcatattgct gttcgcctgt
     6481 ccagccaccg atataatgac cggttgcttt catatatacg taataagtca aaccagaaca
     6541 gtcaaaagta tttggacctt ttgctcccca tacatatggt tttccaagat gcttgaatgc
     6601 ttctcgaacg attgcttgtt ggacattcgt tgtatttgta ggcaatacag catcaattgg
     6661 tgattttaca acaaaatcag aagaagcaga agatggattc tctataccgc tttgatttgc
     6721 tgtatttgga cgagatgaag cattgttcgt tccgccattc gatgaaccat tgtttggatg
     6781 gtttggttct ctctgagtat ttccattgtt attcgatcca gatgggtttg tttgattcga
     6841 agaattcccg ctgttcgaat tattgttatt attgtttgaa ttatttgaac cattattatt
     6901 gttgttatta cccacatctg gtttgtttgg cgtttccgga tctggtgtac tagggacttc
     6961 ttcactagga tcagttggtt tctctgttga agtatccggt ccagctttaa attctgcagt
     7021 aatagataaa ttgttcgtac ctactgtaaa ggtctttgca tcataattta cttctttggc
     7081 accattaaca gcgactgaag caatttgttc accttcagct ggcgtaatag tgtaagttac
     7141 cttcgtttcc gctaataaac cttctaccgt tccactttcc gtaatatcca aaggttgctg
     7201 ttcaggggaa tccgctgtcg aagcagtcag ttggatctta ccaatcaaag aaggagcaat
     7261 tgtaagggaa tatgtctcgg ctggcttttc attttctgat ggatcagccg gatcaatttc
     7321 agttggctcg gtcgatcctg ttgttgagtt agttgttcca gttgggtctt ctgtaaaaga
     7381 atccgttgtt tcactagtag ctgagctgga tgtagtcgta tctgttgtga ctgtttgatc
     7441 agctatttga ttatctacgg caaatacggt attggtcgac aaaacagacg gagcaatcag
     7501 ccaagtagca gctagcaaac gtatcgtttt tttatttttc ataataaact agtccttttc
     7561 ctttcattga ttttctgtct tattcagcat tcattagaca ttttttggaa cagataatcg
     7621 tgtcgaaaag ctttttcttt tcataaatct gtctgtgtat tctaaataaa acaatttgta
     7681 aataagcaac gagcattcat tcgtttttct ttgacgttca ttttattgca tagaccacct
     7741 ttgtttgggg ataaacaagt ggggataaaa atggttatgc atatgtaaat tataccaaag
     7801 tttggaaagt atcacaaaaa tttataaaaa taatgatcct aaaaagaaaa agactgaaat
     7861 atgtgataaa catctcatac ttcagtcttt ttcattaact attcataaga cattaggtct
     7921 tcatcaaacc cgctaacgag agtagtgatc attgctacga ctagactaac agccagtcct
     7981 gctaagtacc agaaaaaagt accggaaatg aatgcaggca atgtggtcag actaccaaat
     8041 acaaaagcga tcgctttcac ttgggcaaat cccattacag ctccaccagc agctgcacca
     8101 atgcaagcag caacaaatgg ttttttcaat gggatcgcaa ttccgtaaac agttggttcg
     8161 gtcactcccg caagtaaaat cgaagcaaaa gcagaaccgg agatcgaacg ggtttttgaa
     8221 cgtttacttc gcagaaatac acctaaagct gcaccagcca ttccaaaatt ggctgctcct
     8281 gtcaacggaa cgattcggtc gaacccataa gcagagatat tactgatcat gacagggttt
     8341 acagcccatt ggataccaaa tattaccaaa atattccaac caccagcgat gattgcgcca
     8401 ctgatcggtc cgttaatatc catcatccaa gtaattgcgg cagctaaacc ttcgccaagg
     8461 taaacacccg caggcccgaa taccattaac gtcaaaggaa ccgtgataac taatgaaagt
     8521 aaaggtacaa atactaattg catattttcg cgaatatatt ttttgaggaa tttttcaaca
     8581 aaagaataaa agaaaatacc taaaatcgcc ggcatgacag tagacgtata ctgcatcagt
     8641 actacaggaa tccccagaaa atcagtagct gctttgccac tgtctgaaag taaagaggtc
     8701 agattcggtt caatcagtgc tgcagcaatg acagccgcaa tatatccatt cactttaaac
     8761 gttttggcag cagaaaaagc aagaaagatt ggcagaaaat aaaagacggc attcgacgca
     8821 gcagacaaaa tatgataagt tccgcttgtt tcactaagcc agccaatcgt agtcgctaaa
     8881 acaaccagac cttttaatat accagaaccg gcaaacagcg gaatgatcgg cgtaaagatt
     8941 tccgatatca aaccaaatag tttttcgatc ggatttagct tttctttcgg agctgaatga
     9001 tcgatttgac gcgtcaattc cttgtaagca ggagccaatc cctgatccat gattatttct
     9061 gcatatctat cgagtaaaat gaaatactta atggaaggga tttgctgttt taatttttct
     9121 gcattggcta ctggctgttt tttcaatttg atacgaagcc gagtcgcaca tggaaggaca
     9181 gaatcaatat tgtcttttcc tcccaactgt tcaatcagca tatgaatctg ctcggacagt
     9241 accatctgtt caggattcat tgtcgtttcc attagaagac cactccactc actaatataa
     9301 tattggcgta aggtgtcatt tcgcctgtac gaataaatgc atgacagtta ttcaactctt
     9361 ttttcatttc ttcatgtggg ataaagctaa tcggtgtatc tggtaacaat aatttgattt
     9421 tttccaatat ttccgggttc attgtcttta tttcttccgc caattcaatc gattgtacct
     9481 caagttcttc taaaacgttt tctaaaacag ataaaaaact tggaagaccg tttgagacag
     9541 ccaaatctat tttttcagta ccaaagggga caggcatgcc tgcatccccg atacttaatt
     9601 tatcaaagtg ccccatttgt gcgattactc gtgaaatatc tgaattgatt acttttcctt
     9661 ttttcatgtt tgtttcttcc tctcattgtt aattttagac tgctgattgt tctaaagctt
     9721 cgcggtaagg gatagaaggt tgcgcaccat aagactgcac agcaacagaa gaggctagat
     9781 ttccataaag gatcgcttct ttcaagttac taaggtctgg ctgaagtgtg ctgcttaatg
     9841 cgccaataaa tgtatcacct gcggctgttg tgtcaacggc atccacttta aaagcaggga
     9901 cgataccgtg ttctttttct gtatgataga aagcgccggc actgcctagt gtaatgatta
     9961 ctgttccaat acctaattca tggagttttt cggctgctgc gaccaaactg ttatgatctg
    10021 tcactctgat tcctgtaatg atttctgttt ctgtttcatt tggtataatg atatctgtct
    10081 tttcaagtag ttcagtagga atcgtttcac gagctggtgc aggattcagg atcgttgttt
    10141 ttcctgcgtc ttttgcaata gaaaaagcta ggatcgtcgc atctaagcta ctttcaaact
    10201 gagcaataat aaagtcactg tttgtaatca gttgcttgtt ttttaggact tcttgttcat
    10261 ggaaagcatt atttgcccct gcatggatca agatactatt ttctcctgct gcgtctacca
    10321 taatcattgc ttgtccggtt gtggcgcctt ggatttcagc tatcccagat gtatcgatgt
    10381 tttcttttgt cagtagatct aataactgtt gtccttcgct gtcagcccca acgcctccaa
    10441 taaaagatgt ccgtgcgcct gaacgtttgg ctgccactgc ttggttggca cctttacctc
    10501 cgccagcgtg aaaaatctca tgcgcatgca tcgtttcgcc tggcttgggc atgttggtta
    10561 aacgaagggt agtgtccata ttaatacttc caattactgt aaccgtgttc attttacaaa
    10621 cctccaacta tctcatttat tttgatggga aaattatttt ttaaaacgtt ttagtaaaac
    10681 gttttaatag ttcgataaaa atatagcaca ccttgaaagc tatttcaagg gtgcagttga
    10741 aaatcttttt tctaatcgta caggtaatct tttttcttcc cattcttttt ctgggaattg
    10801 gatccgatct aacaataact tggcagatgt ttgtcccaat tcgaagattg gttgtgcgat
    10861 cgttgtcagt ttcggcttga tatactcgca catatctata ttatcatagc caataatgct
    10921 gtagtcttca gggatgcttt ttccagcttc ctctaatccg cgatataatc caaaagctaa
    10981 ttcatcattt aaagcaaaca caccagttgc agcagaatca agaagttctg ctgtgatctg
    11041 gtagccacct tgttttgaga actgtgtagg taagaggatc agttggtcgt gagggatttg
    11101 gtaaacatca agagcagatt taaacccctc aatccgagcg tggacatttt ccggtggatt
    11161 ttcaggatag acaagggcga tcgtttgatg tcctagacta agcaagtgca tgcccgctag
    11221 gtaaccaccg cgaaaatcgt ctgttcgtac ggcatcacta aatccttcag attttttctg
    11281 atccaaaaca atgaagggcc ggccttgttt tttcaaattt tcgttgattg catctgtaga
    11341 gacagcagaa gtcgcgataa tgaatccatc cacacctcga cgtgtgagct cggctagata
    11401 ttcgatttct ttttgatgat cagaatcagc attacataat atcgtgacga aattctgctt
    11461 atacaagata tcctcaattc ctcgcatcag tgtactgaag aatggattcg tgatatcagg
    11521 aacgagtaca ccaattgttt tcgtttcctt ggtaatcatt tgtctagcaa aatagtcagg
    11581 ctgatagcct aattcttctt tggcagctaa aactttctga acagttttcg gactgaattt
    11641 ctccgtattt ccatttaata tcaaggagac agttgtgatt gaaacgccgg aaagtttagc
    11701 gacttctttg attgtgatct tttttttcac catgttcata ctcctatcta aatcctgtgg
    11761 ttattttccc atgaaatcaa aaagaagtaa atgaaaatca cgtgtgccag tacttatttg
    11821 ctttgttgga tagcttcgat tatttgtttc gttgagcgta ctcgtgcagt tttcggaaat
    11881 atctttttta tgaacaattg atgggattct ttgtcacgat cactgcaagc atcttcagca
    11941 agcacgatat ggtacccgtg ttgaaaggca tctaatgcag tagcatatac accgtttgtt
    12001 gtcgctacac cgcttaaaat gatagtttcg attccacgcc ggcgaagttg aagatcaaga
    12061 gaagtaccaa aaaaagcact tggattgtat tttgtgatag tcaacatatt atctgtatct
    12121 ttcaaagaat ccttcaaaag taaatcagtg aattctggag gtacagaagc agtcattcca
    12181 cccatatcag tttgttgact caaataaggg aaagtggaga tgtctacgtt cactaaaact
    12241 ttcaaggcag cggtacgttt caatgttctt actaacaaat cattgtttgt cagaatagat
    12301 gctgcagtgt gtggagagag tgtaccgtta tttacgatct ctttttgcat gtcgatcgcg
    12361 acaaaagcag tttttgataa atcaaaaatc atattttcct ttaaatcatt catgatgttc
    12421 cttctttcaa ataacaatgt tttttatgta agtaaatcat aaatcgttta tttgtcaact
    12481 aaattaaagg attcctttct ttagtctaga gaaattcagt ggtaaaataa ataagtgtga
    12541 gaaaacgcat tcgatgcata gatatagcga gaaatatcgt gaaaatggag gaatcagagt
    12601 gaagtatgtc gaagtaaaaa atgaatacaa acgatacaaa atgggagaaa cgaccattac
    12661 agcaaatgac gatatttctt tttcaattga aaaaggcgaa ttagtgatta tacttggtcc
    12721 aagtggagca ggaaagtcaa cgattcttaa tattttaggt gggatggata cacctgatga
    12781 aggtcagatc attattgatg acacagatat tgcgcaattt tcagataaac agcttactgc
    12841 ttaccgacga acagatgtag ggttcgtttt ccaattctac aatctcgttc ctaatctaac
    12901 agcaaaagaa aatgtggaat tggcaacgga agtttctcct gatgcgcttg atcctgtaga
    12961 ggtattgagg caagtgggcc tagctcatcg attgaataac tttccgtccc aattatcagg
    13021 aggcgaacag cagagggttt caattgctcg tgcactagct aaaaatccca aacttctttt
    13081 atgtgatgaa ccgacaggcg cgctggattt tgaaacgggc aagcaagtat tgaaattatt
    13141 gcaaaacgct agcagacaac acggcaatac cgttttgatc attacacata attctgcttt
    13201 agcgcccatc gctgatagag tcattcacat taatgatgct aaagtaaggt ctgttgaatt
    13261 aaatgatcat ccttcatcca ttgatgagat tgtatggtag ggggatttta tgaaaacaag
    13321 aacatatctt aaagcaggtt ttcgcgaaat tcggcaatct aaaggacgat tcattgcaat
    13381 catccttatt gtcatgctag gaacactttt atttgtaggt gtcaaaactg ctggacctgt
    13441 catgcaaaaa acgatggatc actatgttgg tacagcagga ctttctgatt tgcaaatcgt
    13501 ttctactggt ggcttgacac aaaaagatat tgctcaagca gaaaagatcc cagatgcaaa
    13561 agtagaaaca ggaaaacaga tctattattc caatactggc aaaaacgaag tgattcaaat
    13621 attttcttat aacaaaaata acaagcagaa caagctacaa ttaactgatg gaaaactccc
    13681 ggaaaaacca aaccagcttg tattggatga aaaagcggaa gatgaagggt ataaaatcgg
    13741 tgatatttat cagattgatt cagatgaatt gaaagaaaaa gagtatacca tcacgggctt
    13801 tgtccgttct ccattgttca tcaacaattt ggagagagga tacgcgaacg tcggaaatgg
    13861 tagcgtcgat tattttgtct atttacctga atccgttttt aaaacagata tttattctgt
    13921 cttatatctt gattttacaa acgtaaagaa tttgaatacg tacagcaaag cttacaaaga
    13981 taaaatggag caaaatcaag aaaaggcaga aaagtattta caagatcgac ccgaagaacg
    14041 cttagaagag ctgaaaaaat cggccaacga agaattagaa ccggcacagc aaaaaatagc
    14101 ggatggcaaa gcacaaacaa cgcaagcaag aacacggtta gaagatgcta aaaaacagtt
    14161 agcgcaacag tcagcaacta tccagcagct tcctgaagcg caacgaacaa tcgctgttca
    14221 aacattatct caacaagaag ctcaacttgc tgaacaagaa aggcaattag ttgagaaaga
    14281 acgtgaactg gcctctgcag aggaagaatt gaatcgaaat gcagaaaaaa ttgaaaattt
    14341 aactaaacca tcatacctat acaatgaacg ttcagaaaat gtcggttttc aagaatttgg
    14401 tgatctgtct gatcgtatcg ctgcgattgc caatgttttt cctgttttct ttttctttat
    14461 tgccgcattg atcaccttta cgacaatgac acgtatggta gaagaaaacc gcagggaaat
    14521 cgggacatta aaggcgttag gttatacgaa agttgaaatc gcaaaaaaat atgcaatcta
    14581 tgcaagtcta gcctcaggga taggtattgt tttaggaacg atattaggaa cgaatctttt
    14641 gccaaggatc atttttgagc tgtctaacga gcggtatgat attgggagtg cgcttatttt
    14701 ttatgactgg ccgccgattt tacaagcagc tgccgcattc ttcattgccg catttggcgc
    14761 tgcaatgatc gttttattca aagatttaag agaacggcct gctgcattgc ttcaaccgaa
    14821 agcaccgaag ccagggaaac gcatttttct tgaatacatc actccgctgt ggtcacgcct
    14881 tagctttaat caaaaaatca gttaccgaaa tctcttccga tataagtcaa gaatgcttat
    14941 ggcgattatc ggaattgctg gatgtgccgg gctgatggtt gcaggcgtgg gattgaaaga
    15001 ttctctaagt tct
//
LOCUS       DAYLZO010000071        14130 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.072, whole genome shotgun sequence.
ACCESSION   DAYLZO010000071 DAYLZO010000000
VERSION     DAYLZO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.072"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..582
                     /locus_tag="ACGVJG_001961"
     CDS             <1..582
                     /locus_tag="ACGVJG_001961"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFJ6557890.1"
                     /translation="GIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSY
                     LLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVM
                     DVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKV
                     IKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
     gene            615..1577
                     /locus_tag="ACGVJG_001962"
     CDS             615..1577
                     /locus_tag="ACGVJG_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFJ6557891.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            1570..2997
                     /locus_tag="ACGVJG_001963"
     CDS             1570..2997
                     /locus_tag="ACGVJG_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFJ6557892.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            2999..4069
                     /locus_tag="ACGVJG_001964"
     CDS             2999..4069
                     /locus_tag="ACGVJG_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFJ6557893.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            4056..5477
                     /locus_tag="ACGVJG_001965"
     CDS             4056..5477
                     /locus_tag="ACGVJG_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFJ6557894.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            5490..6497
                     /locus_tag="ACGVJG_001966"
     CDS             5490..6497
                     /locus_tag="ACGVJG_001966"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFJ6557895.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            6509..7513
                     /gene="neuB"
                     /locus_tag="ACGVJG_001967"
     CDS             6509..7513
                     /gene="neuB"
                     /locus_tag="ACGVJG_001967"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFJ6557896.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            7510..8658
                     /gene="neuC"
                     /locus_tag="ACGVJG_001968"
     CDS             7510..8658
                     /gene="neuC"
                     /locus_tag="ACGVJG_001968"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFJ6557897.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            8631..9308
                     /locus_tag="ACGVJG_001969"
     CDS             8631..9308
                     /locus_tag="ACGVJG_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFJ6557898.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            9259..10440
                     /locus_tag="ACGVJG_001970"
     CDS             9259..10440
                     /locus_tag="ACGVJG_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFJ6557899.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            10453..11430
                     /locus_tag="ACGVJG_001971"
     CDS             10453..11430
                     /locus_tag="ACGVJG_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFJ6557900.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            11430..12338
                     /locus_tag="ACGVJG_001972"
     CDS             11430..12338
                     /locus_tag="ACGVJG_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFJ6557901.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            12339..13091
                     /locus_tag="ACGVJG_001973"
     CDS             12339..13091
                     /locus_tag="ACGVJG_001973"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFJ6557902.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            13096..14043
                     /locus_tag="ACGVJG_001974"
     CDS             13096..14043
                     /locus_tag="ACGVJG_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFJ6557903.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         5125 a         1732 c         2538 g         4735 t
ORIGIN      
        1 ggtattgatt tagagcttta tagtaaaaat tcagaagcta aggttaataa gagaaaagaa
       61 ttggatatct caatgagtac ttttgttgtg ggaaacattg gacgattaga gaaacaaaaa
      121 aatcaatctt atttgttaga gattttttta gaaatactaa aaatgaattc tgattcttac
      181 ttgattttag taggtgaagg gtcacttaga aataaactga aagccaaagc agtagaattg
      241 gatatagaga aaagagtaaa atttttagga gaaagaaacg atattcccga gttattatct
      301 gtgatggatg tttttgtaat gccttctctt tatgaaggtc tgccaatggt tggattagaa
      361 gcacaagcag caaaattaaa attagtctta gcagatacta tttccaaaga tacaaagtta
      421 acaaataatg tggattttgt atcattagat gaaacaccac aagaatgggc aaaaaaagta
      481 ataaaagagc cgtatgctaa taaaataaca caagaactaa agagttatga tgtttcgtac
      541 actactgaac aaatggagag tatatatagt agagatcatt aaaaatgttc aagatatttt
      601 aggagtgatt atttttgaaa atatgttatt tgatattggc atacaatgat attggcaact
      661 taaagagatt ggtcaatcga ttgaacgaaa atgcagtttt tattattcat atagataaaa
      721 aaactgatat agaaccattt attgaagcat ttaaatcata tgataatgtt gaatttttat
      781 gtaataaaaa ccgtgttaaa atttattggg gtggtttttc cattgtacaa gcagaaatca
      841 atttagtaaa gagagcatta caaaatgaga aatatttaaa atatgtatta ttatctggtg
      901 ctgattatcc aatcaaagat aatgagtata tttataatta ttttaaaaag aatagttcag
      961 tggagtttat tcgtggaata gatctagatc aaattaaaca taaagaattt tattataaac
     1021 atatagatgt atatcaaaaa catgattatc ctagaattaa taggaataac acaactgcat
     1081 ttaaaatttt tagagctata attaatcgtt gtctgaggat gattaaactt cccccaaaaa
     1141 ttagacacca taaatttgat ttgtatcatg gttctcaatg gtgggctttg tcgaaagaat
     1201 gcctaacaga attaatacaa atgtatgaac aaaatcaaca agactacctg aattttaaaa
     1261 taggtatgtt tgccccagat gaaaaatttt ttcatacact tttttttaat tcctcgttca
     1321 aaaataaaaa tgttattgga ggaccagatt taccacttga attgaaaaat attgaagaaa
     1381 ctactttaca aacttcgaaa ttagcaaata ttcatattat tgatccttcc atgaacaaat
     1441 ggtttgatga aagggatata gatcaaataa aagaaagtga aaaattattt gtaagaaaag
     1501 ttaaatcgga ttactctgat aaactattaa atagaattga taaagaaatt ttgaaagttg
     1561 gaaatataga tgaatagaat agtacctgtt ttagagaaga gtttattaag tcgagatgcg
     1621 tttcgagtaa taaatttaat catttgtctg ctttatttta tgcctagtac ttttaatgta
     1681 actaatatcc cgatgaaaat agcttttgct tggggtggaa ttatagtttt gtatgaggtt
     1741 tttataaaga aaaatttttt taaaatgcgt ttttcttggc tactgattgg agcaatgatt
     1801 agttttatct taactataat cattaacaga gaaaattttt taattccatc tatttataat
     1861 tttggatatt tgctgattac tttgatagta atttttcctg cagacttttc acaaagtgaa
     1921 gaggaaaaaa agaaaaaaat ggtgtatttt aataatgttt tcattatcat aattttttta
     1981 gctgcgctaa tatctatcta tcaatttatt tttttgattt cttatcatgt cccaacagga
     2041 acgccaggat tacttgcaag acaaggtttt attgaacatc ggttatttgg tgtctataca
     2101 agtccaaatg ttggagctat gtttggatat acttctatta ttttaagttt aattagtgta
     2161 ttaatttcaa aaataacaaa aacgattcgt gttttttata tgatgaattt tatcgtacaa
     2221 atattttata ttacactttc ttcgtctaga ggtgcccagg tagtaatttg ttctttttta
     2281 gtattatttc ttttgttgtt tgccaattca aaatttagaa agagcattag agaagtacta
     2341 cctgtaaaaa tcaaatggat ggtaagcata ttattagttc tattggtata tttttctttt
     2401 ggtactaatt atgctaagga ttttttagca acaattcctg tatcgattga tagaaaagta
     2461 gaagttatag agtcaaatga atcagataaa actccacttc cagaaaaaga agtaatatta
     2521 cagcattcta gtgataactc cgaaatttca gctggtcgtt ttacgatttg gaaagctgcg
     2581 cttaaagtta taagaccaac tttgttattt ggttatggag agaccgattt ctatgggaat
     2641 agcgatgcaa aatttttgaa aaaagtagat cttgatacta cagatgtgaa tgaactaaaa
     2701 agagcacatg gaaacatgca taatggattt cttcaagtac ttgtatcaag tggtataatt
     2761 gcttttatat gtatctatgc tttctatgtg ttaaatatct ttagtttact caaaacattt
     2821 tttagaagta aagtgaatta tgcgtttctt ggtattgttt taatttttat actatctatt
     2881 tttatagatg aaatggtgga ggctcatgta cttttcaata agcgcgatgt catttcaatt
     2941 gttttctggt attatttggg aagcatttct tacttgtatt taaaaaagga gaattaatat
     3001 gaagacagct ttcattgctt ggactccttt acatttgatt aatatattaa atgtttatca
     3061 aaatttttac cgagaatcaa aaggagattt atttatttat tctgaatttg aaaacgctag
     3121 aagatatagt gagcgtataa aagaattagg gctatttaat gatgttgttt ttgtgaatca
     3181 ccggaaaatc ggttcttcct ttaaacgtaa aattaacttg ttaactaacc ataatcaatt
     3241 tttctcggat gatttatccg tttatgatga aatttttata cagggggaaa attatttcac
     3301 aaaaatactt ttctccaatg tttacaaaaa aaatccagat gtaaaactaa attatataga
     3361 agatggtttg ggtacttatt tgaagaagag tatattttca acaagtaaaa acgttcaaag
     3421 agtttttaaa atattaaata aaaatagcat cttcaaaaaa aaaattgacg attattttgt
     3481 ctattgtccg gaattgttac tttatgatgt aaaaaatata aaaaaaattc ccaaaataga
     3541 tgaaaacagt aaattgatga aaaacataca atcaatcttt gggatagaag aagtttttgt
     3601 cggagataaa tgtatctttt ttgatcaacc tttattatct gatggttatg gcatagatga
     3661 aacagatatt ttcgctaaag taaatagtat attttctgga aaaaaagaaa ctttaattaa
     3721 gcttcatcct agagcaagga caaatcgtta tggtgaaaaa aacatattga attccagttt
     3781 accgtttgaa gtcttgctac ttgaaaatga cttctcttca acacttttga ttagtcctat
     3841 atctacaatt tctttttcac catctatgat gtttgaaaag acgatcaact ctattatgtt
     3901 ggctaaatta attttaaatg aatatacagc attatctgaa gataaaaaag aaacattgac
     3961 aaatattgtt cttttctgtg aacattataa ccgtctatca gaagaaaaaa ttttactccc
     4021 agaaaactgg gcagaattaa gaaagttggt agagtatggg attatttaac acactttcca
     4081 agtcaaaatt agttaagtcc agtttctggt atactatagg gaattttttt attaagggaa
     4141 tttcattcat aactattcct atttttgtaa atattactac tcaagcagaa tatggtttga
     4201 ttaataattt cacttcattt gtatcaattt tttcgattct tgttgggcta agcctaaatt
     4261 cggcaattaa taatgcaagt tttgactata aagatgacat aaatgaattt atgtcatcgg
     4321 tactttctct agctacactt tcgttaattg tgttcttatt aatagggaac atatattttt
     4381 tgttttcaaa tgcttttttt gatatgaacc aacttgtatt taatttgatg ttattacaaa
     4441 gttttgctag ttttgtaatt agttatatcc agtcttattt tacgattaac tttaaccatt
     4501 ttaaatattt gattttatct tttatatcta cttttttgaa tattgcaatc tctgtagctt
     4561 tgatgcagac attatatttt gatgataaat atgttggcag agtagtcggc ggaacactgt
     4621 catttgtgtt gataggttta gtaatttatt taagaataat gataaagggt gctcattttt
     4681 ttgataaaaa acagtggaga tacgctttaa aaatctcttt gccgctaatc ccacattcct
     4741 tagcaaatat agttttagca caattcgata gaattatgat taattcttat ttaggtcctg
     4801 ctgcgggggg aatatattcc tacattagta acataggtat tattctaagc gtaatatgga
     4861 tgtctactaa taacgcctgg gttccatggt tctatggtga gatgagtaaa aataatgaaa
     4921 aaaaagtgaa gaaagttagt aactattatt tagtagtctt tactttgatt acgatgattg
     4981 taatggtagt ttcgattgat ttagcaaaat ttatggctcc gaaagactat tattctgggt
     5041 tatctttagt ggttccgata accttaggct atttctttca gtttttatat agtttaccag
     5101 ttaatgctga attttatgag aaaaaaacat cgtttattgc gattggtaca gtagcaagtg
     5161 ctatagttaa tatactatta aacatgattt tcatacctag gttaggaata attgctgctg
     5221 gatacacaac cgtatttacg tattttttac tttttatttt tcattataat atcgctaaaa
     5281 aaattacgaa taaacaatta tttgacacaa aaaaaatatg tatcataata atttttgtat
     5341 caattttttc tttgatactt catattttaa tcaatcaaat gttgattcgt tacgttttga
     5401 catcattatt gataattagt ttagttatat attttttaaa gattaagaat cgaattatag
     5461 atgactataa ttcataggag gagatataag tgtttaaagg aaaaacaata ttagtaactg
     5521 gcggaactgg ttccattgga agtgaaattg ttcgtcagct tttaaaatat gaaccaaaag
     5581 ctatcagaat ttatagtaga ggtgaagaaa aacaatttta tatgcaacaa gagctctcaa
     5641 atcacactaa tttgagattt ttaattggtg atatcagaga ctctcaaagg attgattatg
     5701 catgtcgtga tgtggatatt atttttcatg ctgccgcaat gaaacacgtt ccagcaagtg
     5761 aatataatcc tatggaagct gtaaaaacaa atgtgatggg aactcaaaat gtaatagatg
     5821 cagcgatcaa aaataatgta aaacattttg taggtatcag tacagataaa gtagtgagtc
     5881 ctactaacac catgggtgcg acaaagttat tgagtgaaaa attaataata gctgccaatc
     5941 agtataaagg aaaaacagaa acaatatttt cttgtgtaag gtttggaaat gtcatggggt
     6001 caagtggatc tgttattcct ctttttagta aacaaataaa aaacaataaa gatattactt
     6061 tgacagatgt tgatatgaca cgatttatga tgtcaatacc ggatgctgca agattagttc
     6121 tgtctgctgc tatgaattca aaatttggag aaacatatgt cttaaaaatg ccggcaatca
     6181 atattttaac tttagctcaa tcattaataa aaatatataa tgatacacat gatgaaaaat
     6241 atagaggtga aataaaagtt gtaggtatca ggccaggaga aaaattatat gaagagctaa
     6301 tgactacaga cgaaattgaa cgctcatatg aaaatgataa aatgtatatc attccaccac
     6361 actatcatgc tactgacgaa ctgtacaaag attatgaaaa aattaaaatc actgcatact
     6421 cttcagatgc aacagaatta atgagtggac aggaatttat tagaatcgca aaagagaata
     6481 atgtcgtgac ttggtaagga gttttatgat gaaatcagaa gtatatgtaa ttgcagaaat
     6541 tggtgtaaac cataatggaa gtctagattt agctttagaa agtattgata aagctaaaga
     6601 atgtggagct aacgcggtta aatttcaaac atttaaaacg aataagctag tttccaaaaa
     6661 ggctccaatg gcaaaatatc agaaggataa tcttaaagag gacagttctc aatttgaaat
     6721 gctaaaaaaa ttagaattat ctcaatctga ttttattaga ataaaagatt attgtataca
     6781 aaataaaatt gattttttgt ctactccttt tgatgaagaa agtgcagact ttctaaaaca
     6841 aattggtgta caaggattta aaattggctc aggagattta acaaatttac cttttcttag
     6901 aaaaatagat atgtatgggt tgccagtttt attatctaca ggtatggctg atattcatga
     6961 ggtcagtgag gctgtagatt gtttcataga tagtccagtt actattttac attgtacttc
     7021 aaattatccg gctagttctg aagatattaa tttattggcg attaacacat taaaagaaat
     7081 gttcgggata cccgttgggt actccgatca ttcattggga tatgatgttg gtatttgtgc
     7141 tgttgcgatg ggtgctaagg taattgagaa acattttacc ttggataagg aactacctgg
     7201 tccagatcat aaagcttctt tagatcctca ggaatttgca gattttgtaa agcatattag
     7261 aaacactgaa atatttttag gtgatggtgt taagcgagca atgccttctg aacttagtac
     7321 aaaagaagta tcaagaaaaa gcctagtggt cactgagaat ttaaatgtgg gagatattct
     7381 tacagagaaa tctcttacaa taaaacgtcc tggaaatgga atagctccta agtttttata
     7441 tcaatatatt ggcaaaaaag ttaaaagacc aataagtgca gatagtgttc tgcttgagga
     7501 agatgttgaa tgaaaaaaat acttatagta agtggaacac gagctgatta tggaatatat
     7561 aaacctgttg ctgagaattt actacaagat aattgggatg tatcatttgt tgtttctgga
     7621 atgcatttat cccataaata tggatttact aaagaattga tcttagctga cggatttaga
     7681 attagtggag aagttcaaag tttgttttta gagaacaata agggaaacat ggctcggtct
     7741 gtggcattag agataaatgg tttcacaaat atatttgaac aaactagccc tgattttgta
     7801 ttattactag gggatagagg agaaatgttg gcagctgcga tagcttgttt gtatcttgga
     7861 atcaaaacag cacatattca cggaggcgaa gtgtctggta ctgtagatga atcaataaga
     7921 catgccataa cgaaaatagc atctgttcat tttgctgcta cggaaaaaag caaagagcgt
     7981 ttaataaaaa tgggggaaga ttcttggaga gtttttcccg tcggagctcc tagaatagat
     8041 actattttaa ataatagatt acctagtttt agagatataa aagaaaaata tcatctaaaa
     8101 tttgaaaaga aaaaatacgt tttgcaagta tttcatccag ttgtaaccga actagaaaat
     8161 atagaaaaac aaattttagc tttgatcaat tcaatggaac aacaatctca accaattatt
     8221 tgcattttac ctaatagtga tgctggtagc gaaattatca gaaaaatgta ccagaaatat
     8281 ttaaaaacgg atattattta tattacgaat ttagagccgg aggaatacct gactatatta
     8341 aaagagtgca aatttatgat cggaaattct tcttctggga taattgaagc tgcatcattc
     8401 aaaataccag taataaattt aggaaccaga caaaatggac gagaacaatc taaaaatatt
     8461 ataaatgcaa atctaaatga aaatgaaatt gaaactgctt tgaataaaat acaagatcct
     8521 cagtttataa aaagcttgaa aaatgttaag aacatctacg gtgacggcaa cagttctaga
     8581 agaataacaa aaacattgaa tcaaatattg aatgatgggg aagatattaa gtggattcaa
     8641 aaacaaatag cttattaaaa tatactagtg atccatacat tattattggg gctggcggac
     8701 acgcaaaagt cattattgaa accttaagat tagaaaataa gttaatatat ggattttttg
     8761 atgataagca aaataaaatg caagatctga aatatcttgg gaaaataaac gaattgaata
     8821 gctgggcgga tagcaaaaaa aaaatctata aatttattgt agctattggt gactgtgtag
     8881 taagaaaaaa gattataaaa aaattgaata tgccaagtga gcaatatgga atagctgttc
     8941 atccaagtgc tatagtggca aaagaaattt caattggtta tggaactgtt atttttgcga
     9001 atgctgtaat taacccagat gctgtaattg gagagcatgc tataataaac acaggtagca
     9061 ttgttgagca tgattgtaga attaataact atgttcattt atctccaggt gtatgcttag
     9121 cgggtggagt acatgtaggt gtaggaactc aaataggaat tggttctcaa tgtatccaga
     9181 tgaagaaaat tggatcttgg tgtatgatag gcgcagggag cactattgtt aaggatattc
     9241 cctcacattc cttagcttat ggcaatccag ctaaaattaa gaaagaagga attaattttg
     9301 aattctagaa ttttattatc aagtcctcat atgggaggaa cagaagaaaa atatgttgct
     9361 aaggctttta aagataattg gatagcgcca ttaggtgaaa atgtggataa atttgaagaa
     9421 agtattacaa aatatactgg ttctaagggc ggagcttctg cgttaagttc aggtacagct
     9481 gccatacatc ttgccttaag actgcttaat gttggaaaaa acgatattgt ctttgctcct
     9541 tcatttactt ttatagctac agtaaatcct attttatatc aaggggctgt accagtgttc
     9601 attgactcag aacctaatac atggaatatg agccctgttg cactagagag cgcattagaa
     9661 gaagctgaaa gaaacaacca attacctaag gcaatcatcg tagtgaattt atatggtcaa
     9721 atggctgatt atgaacgaat tttagctatt gcaaaccaat ttaatgttcc agtgatagaa
     9781 gatgcagcgg aatcgttagg ttctacttat aagggaattt cgtcaggctt atttgggaca
     9841 attgggatct attcatttaa cggtaataag atcatcacaa cctcaggtgg aggagctcta
     9901 gtgtctagtg ataaaaaata tgttgagcat tcaagatttt tagcaacaca agcaagagat
     9961 caagcaccgt attatcaaca tagcgaaata ggttataatt accgtctaag caatatttct
    10021 gccggtatcg gacgtggaca aatggaagta ttgaatgata gagtagctaa acgaagagaa
    10081 attttcaatc gatataagga agctttttct ggaattgata gaattgaaat gatgccagaa
    10141 gtggatggca tggtgccaaa tcgttggttg tctactttaa cgatcaatga ttataattct
    10201 attgatcaag tagttaacgt aataaataaa ctaaatgaaa aaaacattga aacaagagct
    10261 ttgtggaaac cactacattt gcaaccagta tttcaagggg taaaaatgta ttaccatgat
    10321 aataatctta gtgtgtcaga aaagttattt aatagcggct tatgcttacc ttctggaagt
    10381 aatatgacag atagtgaaca agaatttgtt attgatgagt ttaaaaaaat attcaagtag
    10441 gtgtgatatt ttatgaacat aggaattatt ggtttaggtt ccatggggaa aagaagactt
    10501 agactcattc aagaacactt taatgatatt ttactatgtg gtgttgatac taatgaggcg
    10561 agacgaaagg aagttgaaaa ggaatttgga ataccaactt ataatagtct aacagaggca
    10621 aaaaatttgt ttgaaataaa ttcagttttt gtttgtacat cccctataac ccatgaattt
    10681 attataaaag aggctttaaa tttagattgt aatatcttta cggaaatcaa tttattaaat
    10741 gaatattatg atgaaataat cgaagtagct aaaaagaaag atagattact ttatctatct
    10801 tctacatttt tgaagagaaa agaaattcaa tatattaagt ctaaagtgac gcataataaa
    10861 aaaatttctt atcaatacca tgtaggacag tatttacccg actggcatcc ttgggaagat
    10921 tatcgcaatt tttttgtttc aaacaaaaaa actaatgggt gtagagaaat attcgctatt
    10981 gaattacctt ggattataga tacatttggg aaagttaaat cgtttactgt aatgacccag
    11041 aaattatcca atcttgatat tgattattcg gacagtttca ctcttattat tgagcatgaa
    11101 actggtatag ttggaacttt aagtgtagat gttgtttcaa gagtagctaa aagaaattta
    11161 actataattg gtgaaaagat tttaatagaa tgggaaggta acccggattc tctattttca
    11221 tggaatgaag aaacttctag tatgacagat attcaattgt acgatgaggt tatgcatgat
    11281 aataattatt cacatactat tattgaagat gcatatttag aggagattaa agaatttttc
    11341 acgcttttgg aaaagaaaat ggaaccccaa tatacttttg aaaaagataa gtatattatt
    11401 aacttaatta acgacattga ggtaaattaa tgatgaaagt aatttttttt gggttagggt
    11461 caattggaaa gagacatcta aaaaatctaa taagctatgg aaaaaagaat tctattaatt
    11521 ttgatataac agcttatagt agcagtggaa aaaaagagtt tgaagaaata acgtatattc
    11581 aaaatagaga cgatttggat acacattatg atatagcttt tattacaaat ccgactttta
    11641 agcatctgga aacactaaat gtaattgttg aaaaagttga ttacatattt ttagaaaaac
    11701 ctgctttttc tgagagtatt gatcttactc aatataatta tgattacaaa aatatttatg
    11761 ttgctgctcc actgaggtat aaaaatatca tggaagttgt tcgtaatagt attcaaagta
    11821 aaaaagtata ttctgtaaga gctatttgtt ctacttatct tcctgattgg agaaaaggta
    11881 ctgattatag aaataattat tctgccttta aatctatggg gggaggagta gaattagact
    11941 gtatacatga attggattat attataaacc tatttggttt ccctaaatca gtaaaaaaac
    12001 agtttggtaa atattcgaat cttgaaatta acagtaatga tttagcaaca tatatattga
    12061 gatacgatga taaaattata gaaattcatt tagattattt tggaagagtt cctacaagaa
    12121 aatttcaatt gtttacttta gatgaatgta tcaatgttga cttattaaat aataaaataa
    12181 gttttgaaga aaacattgtt aaagaaatgc ctgaatctgc taatgatatg tatgagaagg
    12241 agttgaaata tttctttgaa aacgtagtaa caggcaaaga aaactggaat gatttatatc
    12301 atgcaaacga agtattgaaa atagctgagg agaaataaat ggaaatttta tttactattt
    12361 gtggtagagc tgggtctaag gggtttaaaa ataaaaattt gaaatatttg gcaggaaagc
    12421 ccctagtcca tttcgcttta gcaatgatag atatctatca acaaaaacat cctgaacata
    12481 attgcacgac ttgtttgaat acggacagta atgagttagt agaattgacg actaagttaa
    12541 atccgtctgt gaaaattgta gaacgtaaac ctgaattagc tacagatact gtagggaaaa
    12601 ttgaagtgat acgtgattca ttcttacaac tagaaagaga agataatata tttgatttaa
    12661 ttatagattt agatttgact tcacctttac gtgaagtttc taatcttgaa gaattaatag
    12721 atgaacatct aaaaaattta gataaagacg tctacttttc agttgtttct gcaagacgta
    12781 atccctattt taatatggta aaaagaattg aaaatgaagt tactatagtt aataaatcaa
    12841 gatacacatc taggcaacag acacctgaag tttttgaaat gaatgcctca atgtattctt
    12901 ttaaacctga atttctaaaa aaaagtaatt atatttttga tgggcaatgt gggataataa
    12961 aaatgagaga ttatctaatt ttagatattg atagtgaaga agattttaaa tggttagaat
    13021 ttttgtttcc taaattttta gaagattctt cggagcttca agatatctat aattatttat
    13081 atcggaggta aaaggatgag ctgttatctt atcaccggtg gggctggctt cattggttct
    13141 accttagcca atcatttagg agaagaacat gtggtaatcg tcgtcgatga cttatcaatg
    13201 ggaaaagtag aaaatcttga tatgacgaaa aatatcactt ttattgaggg agatgtcgct
    13261 gagaaatcct tgatggaaga gatcatgaaa gctaataaat ttgattatat ctttcattta
    13321 gcagcagttg ctagtgtggc agattcagtt gcccgaccag tagaaacaca tcgtgtgaat
    13381 tttgaaagtg tattaatgat tttagaattg atccgcaagt atcaacctga tttaaaacgg
    13441 atcgtgttta gttcttcagc ggctgtttat ggggatgaac cgactttacc aaaaaaagaa
    13501 gagtccgtga ttcgcccatt gacgccttat gcggttgata aattcgctgc agaacaatat
    13561 gtgctagatt attgtcattt atatgatgta cccggtagcg cagttcgttt tttcaatgtc
    13621 tatggtccaa atcaaaatcc gaattctcca tacagtggtg tgatttctat tctcgtagat
    13681 cgctataaga aacaaatcgt aggagaagca acttcattta cattatttgg tgatggaagc
    13741 cagtctagag actttgttta tatagatgat gtaatccaag cattactttt agttgcgaat
    13801 gaggaagctg cactggggaa acaattcaat gttggaacgg gtaaagcaac cacacttttg
    13861 gaattgattc atgcaatcaa tgaaattctc ggtgtagaat tgacacttga atacaaagaa
    13921 gagcgttcgg gtgatatcca tgactctcta gcagatatta ctaagatcca gtcaattggc
    13981 tatcaaccga aatatggtat tttaagtgga atgactaatt atttgaaaac tgaaatcaat
    14041 taaaacatac taagataaga ttagagggta aattcaggct ctttgtcaat aaggactgat
    14101 gagttttgta aaatcaaatc tgggatagaa
//
LOCUS       DAYLZO010000072        14075 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.073, whole genome shotgun sequence.
ACCESSION   DAYLZO010000072 DAYLZO010000000
VERSION     DAYLZO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.073"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            29..2194
                     /gene="acm"
                     /locus_tag="ACGVJG_001975"
     CDS             29..2194
                     /gene="acm"
                     /locus_tag="ACGVJG_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFJ6557904.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2466..2951)
                     /locus_tag="ACGVJG_001976"
     CDS             complement(2466..2951)
                     /locus_tag="ACGVJG_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFJ6557905.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3041..3386
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3431..4072
                     /locus_tag="ACGVJG_001977"
     CDS             3431..4072
                     /locus_tag="ACGVJG_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFJ6557906.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4091..4726
                     /locus_tag="ACGVJG_001978"
     CDS             4091..4726
                     /locus_tag="ACGVJG_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557907.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4744..5559
                     /locus_tag="ACGVJG_001979"
     CDS             4744..5559
                     /locus_tag="ACGVJG_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFJ6557908.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5878..7314
                     /locus_tag="ACGVJG_001980"
                     /pseudo
     CDS             5878..7314
                     /locus_tag="ACGVJG_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            7503..9950
                     /locus_tag="ACGVJG_001981"
     CDS             7503..9950
                     /locus_tag="ACGVJG_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFJ6557909.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10180..11247
                     /gene="rlmN"
                     /locus_tag="ACGVJG_001982"
     CDS             10180..11247
                     /gene="rlmN"
                     /locus_tag="ACGVJG_001982"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFJ6557910.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11237..11443
                     /locus_tag="ACGVJG_001983"
     CDS             11237..11443
                     /locus_tag="ACGVJG_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557911.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11440..12543)
                     /locus_tag="ACGVJG_001984"
     CDS             complement(11440..12543)
                     /locus_tag="ACGVJG_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFJ6557912.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13095..13643
                     /gene="aac(6')"
                     /locus_tag="ACGVJG_001985"
     CDS             13095..13643
                     /gene="aac(6')"
                     /locus_tag="ACGVJG_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFJ6557913.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(13891..14010)
                     /locus_tag="ACGVJG_001986"
     CDS             complement(13891..14010)
                     /locus_tag="ACGVJG_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFJ6557914.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         4815 a         2340 c         2967 g         3953 t
ORIGIN      
        1 tttagttaaa ataggggaga tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat
       61 gttatttatt attactaata taacatcact gattcctgtt catgtctatg cggatgcagg
      121 cagagatatc agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg
      181 aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga
      241 ttatctttgg atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga
      301 aatcccatta atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc
      361 gcaggttgtg tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt
      421 tgagatacag gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt
      481 gacgagcggt gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc
      541 tagcgttttt tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg
      601 gtttttgaat attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga
      661 aatccaaagt ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg
      721 tgaacagaag gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag
      781 cgctacattt aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt
      841 tgtcaatctt aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat
      901 aaattttgag aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg
      961 agaaagtttc aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt
     1021 tagaggcgaa ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt
     1081 gactttcgaa ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt
     1141 gacttcgaat gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt
     1201 aaaagaaaaa gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa
     1261 attctccata aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa
     1321 ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat
     1381 ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac
     1441 aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga
     1501 tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt
     1561 cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt
     1621 catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc
     1681 gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag
     1741 tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga
     1801 aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta
     1861 taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag gtagccatat
     1921 aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc
     1981 tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat
     2041 tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac
     2101 aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttgatagtaa ctatctgtgc
     2161 tgggatattc ttacaaaaat ataaaaaagt ttagttagaa tataatattt tttgttaaat
     2221 atgactgaaa tcaaaagaat aaaattccga tttataatcg aaaaaagaag catttttata
     2281 tatgcttctt ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc
     2341 gtattagttt tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct
     2401 gatatatttt gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc
     2461 tatcattaca aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag
     2521 tcggaaaaat ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct
     2581 tgacgatcaa tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat
     2641 gctaatggag tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa
     2701 acaaaagata agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt
     2761 tcatcaggca agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt
     2821 tttacttgcg gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga
     2881 tcgacttttt gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt
     2941 gcacgactca tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac
     3001 aaatgaaaaa taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag
     3061 agtagccgtt aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg
     3121 tgaaacacat cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt
     3181 tacagctgcg gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta
     3241 aaaaaaggtg gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt
     3301 ttcatctgaa cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt
     3361 ggaaccacgt ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga
     3421 ggaaaagaag atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac
     3481 aacattgaaa ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc
     3541 attaggattg tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt
     3601 tatgcgggga acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat
     3661 tgggatcgtt tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc
     3721 ttattttgca gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc
     3781 agcgcaagtt ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt
     3841 gacaaagatt gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc
     3901 cttgatctat atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg
     3961 cgacgcgact ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat
     4021 tctaacaatc gtaatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg
     4081 aggaagaaat atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat
     4141 caaagatttg aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg
     4201 tggcggtaaa accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga
     4261 attttattta gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt
     4321 aggagtcgta ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac
     4381 cttagcacca aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga
     4441 attagcggct tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg
     4501 cggacaaaaa cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc
     4561 ttatgatgaa ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat
     4621 cctatcattg aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc
     4681 agaaaatgtc ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata
     4741 gagatgaaaa aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc
     4801 tgttcgaaca aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc
     4861 gttataggtt tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc
     4921 acaggatttg atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac
     4981 tttcagccca ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg
     5041 atatggaacg gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct
     5101 tatatggaaa acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga
     5161 caaatgaata ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg
     5221 aaacaaccag aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt
     5281 tttaatgaag cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa
     5341 gtatacgcag actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa
     5401 ggttcttatg agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg
     5461 aaaaaaatca atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc
     5521 gataaatggt ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa
     5581 agacgtgtca cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact
     5641 cggagctgaa tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttttcg
     5701 gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta
     5761 ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg ttgtggaagg
     5821 ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa gagatgaatg
     5881 gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc ttctgcagcg
     5941 tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg ggatcgattt
     6001 gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt agaccactat
     6061 catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc
     6121 tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca agctgggctc
     6181 gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc tatgttgact
     6241 ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg ggaatcacgt
     6301 cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt ccgcgggaaa
     6361 gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg ttatttgtta
     6421 gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa ccatattgct
     6481 aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc tggaaaaatc
     6541 ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga aaatgttctg
     6601 gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta tatgtgggga
     6661 aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc aacgattgaa
     6721 gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat caattactat
     6781 caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa aatgaatacg
     6841 acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa aaaagttgag
     6901 aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg attaaggatc
     6961 ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga aaatggctta
     7021 ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg aatcaattac
     7081 ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga agtcctaggc
     7141 tattgcacct ggtcttatac agatttgctt agctggctga acggttatca aaagcgatat
     7201 ggatttgtat atgttgatta agacgaaaca caagaaggat ctttggaacg atataagaaa
     7261 gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct ttagttcaca
     7321 tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata aaatcattta
     7381 gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag ttctttcaaa
     7441 caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat gagggataat
     7501 aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc
     7561 aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg attttatttg
     7621 ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac ggctttcgga
     7681 tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta ggattttgga
     7741 ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt ggcggcatac
     7801 tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa ctccatactg
     7861 gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata aaacaaagtc
     7921 aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat tttacttact
     7981 tcttgattgc acagcctgga agttatttag cagcagtgct attactggca ggaggagcat
     8041 tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca attggagaac
     8101 gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg
     8161 aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct gcaaaagaag
     8221 ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa
     8281 atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc
     8341 caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata
     8401 caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta
     8461 cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag
     8521 ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa
     8581 aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca
     8641 aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag
     8701 taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc
     8761 ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga
     8821 tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac
     8881 tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga
     8941 tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga
     9001 ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc
     9061 cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga
     9121 cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat
     9181 atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac
     9241 agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag
     9301 atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt
     9361 tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg
     9421 tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat
     9481 caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc
     9541 gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat
     9601 tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc
     9661 attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc
     9721 cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta
     9781 tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg
     9841 aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca
     9901 ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg
     9961 atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa
    10021 aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg
    10081 agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat
    10141 agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat
    10201 ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga
    10261 gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg
    10321 aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa
    10381 cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat
    10441 aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg
    10501 acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac
    10561 cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat
    10621 gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt
    10681 gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca
    10741 attggtgctc ggcacattac agtttccact agtggattag cacacaaaat caaggaattc
    10801 gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg
    10861 cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg
    10921 gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa
    10981 gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa
    11041 ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc
    11101 agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt
    11161 gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag
    11221 caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa
    11281 aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc
    11341 gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa
    11401 tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc
    11461 ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt
    11521 ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa
    11581 gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg
    11641 gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa
    11701 taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag
    11761 aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa
    11821 aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga
    11881 gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat
    11941 gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc
    12001 agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt
    12061 atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg
    12121 aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg
    12181 cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat
    12241 gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg
    12301 gacactttca tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca
    12361 gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt
    12421 tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc
    12481 atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact
    12541 catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct
    12601 gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg
    12661 gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg
    12721 aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt
    12781 tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc
    12841 aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag
    12901 ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta
    12961 taatttttta ttttataagt aagataaata atgaaagaat agagatactg attggtagct
    13021 aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac
    13081 aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc
    13141 agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag
    13201 aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt
    13261 tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat
    13321 tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag
    13381 aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc
    13441 atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca
    13501 tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag
    13561 gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta
    13621 ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga
    13681 aataagaagg aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc
    13741 gaagaagcta cttttggaac accgtgtacc agaggttgtg aaaaagtcgc ttagtttcga
    13801 gtagtaagaa aaattttgtg aaaatggctt tctacatttt tgcataaatt tggcttaata
    13861 ccgagaaact ggcttttgaa caccgtttat tcagataagg gactgtgaca agacttttgt
    13921 cacagtttct ttttgtttat tcttttttat ctttgtatgg caataataga caatataacg
    13981 aaaattattt aatggctttt ggaagaccat ggaaaacctt gtacaaagct attttttctg
    14041 aaaaattcct tgaaacctca cgaaaaaatc attat
//
LOCUS       DAYLZO010000073        13861 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.074, whole genome shotgun sequence.
ACCESSION   DAYLZO010000073 DAYLZO010000000
VERSION     DAYLZO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.074"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..306
                     /locus_tag="ACGVJG_001987"
     CDS             <1..306
                     /locus_tag="ACGVJG_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFJ6557915.1"
                     /translation="MGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSNDGVYRYK
                     VTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVVQGELQ"
     gene            359..2335
                     /locus_tag="ACGVJG_001988"
     CDS             359..2335
                     /locus_tag="ACGVJG_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFJ6557916.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            2348..3100
                     /locus_tag="ACGVJG_001989"
     CDS             2348..3100
                     /locus_tag="ACGVJG_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFJ6557917.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            3116..3877
                     /locus_tag="ACGVJG_001990"
     CDS             3116..3877
                     /locus_tag="ACGVJG_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFJ6557918.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            3890..4150
                     /locus_tag="ACGVJG_001991"
     CDS             3890..4150
                     /locus_tag="ACGVJG_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557919.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            4147..6237
                     /locus_tag="ACGVJG_001992"
     CDS             4147..6237
                     /locus_tag="ACGVJG_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFJ6557920.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(6273..8330)
                     /locus_tag="ACGVJG_001993"
     CDS             complement(6273..8330)
                     /locus_tag="ACGVJG_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFJ6557921.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(8475..8666)
                     /locus_tag="ACGVJG_001994"
     CDS             complement(8475..8666)
                     /locus_tag="ACGVJG_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557922.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            8854..9309
                     /locus_tag="ACGVJG_001995"
     CDS             8854..9309
                     /locus_tag="ACGVJG_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557923.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVSYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            9306..9548
                     /locus_tag="ACGVJG_001996"
     CDS             9306..9548
                     /locus_tag="ACGVJG_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFJ6557924.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            9566..9868
                     /locus_tag="ACGVJG_001997"
     CDS             9566..9868
                     /locus_tag="ACGVJG_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557925.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            9937..11991
                     /locus_tag="ACGVJG_001998"
     CDS             9937..11991
                     /locus_tag="ACGVJG_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557926.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            11991..>13861
                     /locus_tag="ACGVJG_001999"
     CDS             11991..>13861
                     /locus_tag="ACGVJG_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFJ6557927.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNM"
BASE COUNT         4900 a         2243 c         2812 g         3906 t
ORIGIN      
        1 atgggtaaag gaaactatgt cctagcaggt cataacatgg aaatgccagg tgttttattt
       61 tctgatattc aaaagttgtc attaggggag gtgatggact tagttagtaa cgatggcgtc
      121 tatcgttaca aggtaacgcg taaatttatc gtacctgagt actttaaatt aatcgatggc
      181 gttcctgaag agaactcttt cttgtcttta ccaaagaaag gagagaaacc cttattaacg
      241 ttgtttactt gtgtttatac gtcacaagga aaagaaagat acgtggtaca gggagagcta
      301 caataacaag aatttaatca acgcgagtcg ttgaaatatg aaatgaggag agtaaaaaat
      361 ggcaataaat cggtatgtaa aaaaagctgg aatgattgtt ggaatgttag gaatcattgc
      421 aggctgcttt caggggtatc gagcttatgc agaagatgtc acgcaaaaaa cgccaccaga
      481 aagggtcaat atcacggttc ataaattaat gtacgatcaa agtacgcaat tgaacgttga
      541 tattgacgga atcaaaaatg atggttatac gcacgatcaa tatcctacag gtgtaacgaa
      601 atacaacaag gcagattatg gtgatgttga gtttacgtta ggaaatatta cggatcaagt
      661 attaccgact gaagattctg atctaaccaa cgcgaaagtg gacgaaatcg ttaaagatgt
      721 agaagacaag ggatctaaca gtgagtacgt taagaatgca accaataaaa ttacttcagc
      781 tgtagatgaa aatggtgaga tcacttttgc tgatcaacca gcctatgtca attccaaagg
      841 gaatgtatat gtggtttatg aaagtaaatc cgctgctgga ttagtcacac aaaaagcaaa
      901 accaatggtt gtgattgcgc caatgacaga caacacagga tcaggatttt taaaagatat
      961 tcatttgtat ccaaagaaca ttgtttctaa gttgagtttt gaattaacga aatttggtga
     1021 cgatggcaca gctcaaagca aacaaacacc tttaaaaggg gcaaaatttg agttatacaa
     1081 gggcgaacct ggaaaaggaa caaaattagg agagcttgta tcggatgatc aaggaaagct
     1141 gactgcaact gatttgacgt taggaaaata ctactttgtg gaagttcctt cagaagttgt
     1201 agttggatca gatcaagaac caacagcaga ccaatatttg ttaggcgcag acgctcgcaa
     1261 cgatgcgcac aataagttaa cctttgaaat tacgaatgac ggcgtgacaa gtgatttaaa
     1321 agcttcctac gtgaactata aagcaccagt gatcgataaa acagtgacaa atggaacagg
     1381 agaagaacat tctttccaaa ttggtgacgc agtgaattat caaggtacaa ttcatatccc
     1441 tacagatatc gcaggtggaa cagatgggat tacagtaaat ggtgtaaaaa gtgaaacttc
     1501 tccttacagt gtatttaaat ggggcgacac agcaggtaaa ggactttctt atgttgctga
     1561 aaaagcagat attaaagtaa caaataatga tggttcagtc gtattaaaag aaaatactga
     1621 ctataaaatc gaaaattctg atagtggttt tgtgatcgat tttatcgtaa ataatggaca
     1681 agtaagtgat acagttgcga atttacatgg tcaagatcta aaaatgactt ataacatgtt
     1741 tgtgaatgat agtgctgaaa tcgcaaatcc attaacaaat agcgtggatt tcgtttataa
     1801 caataatcca tttaatcagg aagaacacca cgaaaaaacg aaagctgacg ttgtgactta
     1861 tggcgcaaaa ttcttaaaag tcgatagcgg cctatttgga acaggtatca aagcaacacc
     1921 gcttgaaggc gcagaatttg cagctaagaa tgcagaaggt aagtactacg gtggacttac
     1981 tgatacagat aaagatggtg tcaaagaagc agtttgggta gacgatgtag caaatgcagc
     2041 aatcctaaaa tcagataaag agggtcattt tgaaatcact ggtttaactg aaggagatta
     2101 ctcactggaa gaaacaaaag ctcctgaaaa ctatcaaaaa ttaacaaaag agatctcttt
     2161 caaagtagac aaagattcat acaaagaaga aaaccgaatc acgatcaaaa ataatcaaaa
     2221 agcttccgta ccaatgactg gtagcaacgg tttccaatct tacgtattga tctcttgctt
     2281 gttgcttggg gcaggcgcaa tttctgcagt tgtttacttt aagaagaaag cctagaagga
     2341 tcagctaatg attatcagac accctaagaa aaagcgaatc atgggtaaat ggatcattgc
     2401 tttctggtta ctttcagctg ttggtgtctt acttttgatg cctgctgaag cgagcgtggc
     2461 gaaataccaa caaaatcagc agattgccgc aatcgatcga acaggaacgg cagcagaaac
     2521 ggattcttct ttggatgttg ctaagattga gttaggcgat cctgttggta ttcttacaat
     2581 tccaagtatt tctttaaaac taccaattta tgacggaact tccgacaaaa ttttagagaa
     2641 tggtgtaggg attacggaag gaactggcga tattacagga ggaaacggta aaaatccttt
     2701 aatcgcaggg cacagtggac tttataaaga taatcttttc gatgatttgc cttcagttaa
     2761 aaagggagaa aagttttata tcaaagttga tggtgagcag cacgcttatc aaatcgatcg
     2821 gattgaggaa gtgcaaaaag acgaactaca aagaaacttt gtcacttact tagaaccaaa
     2881 tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct aaggggatca acacacaccg
     2941 tttcctagtg tatgggaagc gagtaacctt tacaaaatca gaattaaaag atgaagaaaa
     3001 taagaaacaa aaattatctt ggaaatggct tttaggaagt actgtttttc tttcagtcat
     3061 gatcattggt tcgctatttg tttataaaaa gaaaaaataa aggagggtaa ccaacatgag
     3121 aaatagaaaa tggctatttg tttgtgcagc cgctcttttt tctttcactg cctacacaca
     3181 gttgacgttt gcagacagac aagaaagcac ggatagcaca gaacaaacaa tccaaacgtc
     3241 agatagcagc acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag ttgattcttc
     3301 agaagaacct aaaattgctg aagacgatca aatagtcaaa cagaccattc acgttcaaaa
     3361 aattatttct tccgaacaat tagacaacca ggggcaaaag cttgaaaaac aagaaggagt
     3421 aaatggcgcg aagttttctg tttatgatgt atcggacatt ttacaaaaga tggatgttaa
     3481 ggatctcact actgaccaaa ttgaatccca attaaaagat cgagtgaaaa aactatccag
     3541 tgatcaactg aagctaattt ctaatggaga aacaaagaca attgatcaac aaacaggtgt
     3601 ctttgaattc tcggtagaag tacaggcaaa tcagaaacaa gcttattata ttgtcaatga
     3661 atcaagtcct gaaaacattt ctaatagcga ggatattttg ctattaacac ctgtttctga
     3721 taaaaatggt gaattcttaa aggatgtgtg gatttatcct aaatcagaag cttcgcaacc
     3781 taaagaagaa gtaaaaaaaa tcgtatctac tggcgtaaag aaaaacttct ttgagaattg
     3841 ttgggatttt gttactcact tgttttctag agattaagga agaaataaaa tgaaactgat
     3901 caaattatta ttattcaatt tttcttgttt aaccgcagtc tttttgtact gcggtttatt
     3961 attttctaaa ctatctattt tgccaagcta ccaactatac ctaatgagga ttttaaacat
     4021 accagctgct tccatgccgc tagatattat ggaaaagctc attaggaatc cattatttac
     4081 tacgatcaat ttgatcgtta ttgtcggaat ttcactagtc atttataaaa aagaggggat
     4141 gaagggatga attttaaaca ctcgtggaca agacggttaa aatatgtttt tcccataatg
     4201 atgatgttag gtacattgtt tgggttaaaa acagccaatg tctttgctga aaaagttatt
     4261 gtcgatccgt caaatccaat ccagaacgta aagaataatt ttattattcc aaaagatgtt
     4321 cctaatgcaa aaacaaatat tactgtaaac ggagcgtcaa aagtttacaa atatcatgtt
     4381 gatgccaaca aaggtggttt tacagacgac tacgtaggat tagtggaggg aaacaaaaat
     4441 atacgctatc cttctttcca aaaagaatac ggtgaaacaa accttgtttt agaaggcgta
     4501 acaaccaaca ccaaagtcaa tattgactat ggaaaaatcg gtacgtataa cgggaaaaaa
     4561 gtcaatatca agctagttct atctaatatt cacttatact cggatacgtt gccgtggaat
     4621 ctactagata acaactatac aaaaactcat tttcgagacg atggctataa aaatacgaat
     4681 ggtgcaatga gtaaatctaa aaaaagaaca gtactttgga tctcagacaa tcttttctca
     4741 ggaattgttt accattcaac acaaatgaat gtgcagctag tagccacata tgaggacggt
     4801 agccccgttc aattttcagg agatacattt atcagtttta actctttgaa ccctgcagga
     4861 ggaaaaagca cggatcttaa aggagaatat gcccactatg acaaaatgaa tacgacagat
     4921 tggtatgtca gaaaagatac ggttctttca gaatttaaat ctttctacaa caatctgaac
     4981 gtggtaggtg gccacccagg aggaagctct aagctgacac aagcggataa tgatttcaat
     5041 aatttgcatg ataaattagg agatccgaag tttggtcaag gtactgtgtc ctttaaaata
     5101 tctgaagcaa atccgacgtt tgtgattggt tcttctaatg ttcaaacctg gtttacttta
     5161 tcttctgcga caatctttag tgttgttcct gatcaacctg aaaaaacagg ggttgataaa
     5221 aatggaaata acgtgaacga taaaatgttg caagtaggag acacgattca atatcgaatc
     5281 aaacagaagg tgaatcgatt aggagtggat ttactagcgg tttacgatcg atttgagtta
     5341 atcgataatt taccaaaaga agtaaattac gtcgatgcac acgttgaaag tggtacaaat
     5401 aaaaaattcg atgtatctgg tgaagtgacc tatgacaaaa caaaacacca ggtaaaatat
     5461 gcagcgaaag ctgatacatt aaaaaatcga atgaagtata acggcgaaac ttacgaatta
     5521 gtgatcaatg taaaagtcaa tgaattggcc aatcaaaata gtgtagccaa aaatcaggga
     5581 acgtcgatca ttaataaagt agaaaaggat acaaatatca ttaccgttta tttccctaaa
     5641 attccagtga aagaagtaca acaaaatgga aaagatgtaa acggacgaaa tgacggtaag
     5701 aagggtacgc cgacagctcc tttaaacgca ggatctgaag tacaatatct agtcacacaa
     5761 aaatggcaca caaaaggcgt agacgctgcg tcagatcact acaaacaatt tagtattcaa
     5821 gatccaattg aagctcgttt gacatataaa gaaggttctg cgcaagtaat agataagagt
     5881 aaaggaaaag atattacttc tgaaggcaca ctaacctatg atagcaattc aagaacgctg
     5941 aagtgggaag cttccgctga cttcttaaat aacaatcttt tagatggacg agaaattcag
     6001 ctgatattta cagctaaaac tccattacaa tcagaaaaaa atattgataa ccaagccgta
     6061 gtagcagtag ataatgtttc taataaaaca aatgttgtga caattggggt tgatcctaac
     6121 ttgccacaag ttattgttcc taaaacaggt tctacacatt tagtaacgat tttagcggtc
     6181 agcttactat tacttgtgtt agcaactttt ggctacgcag ttctgaagtt caattaaaca
     6241 aaaaaacaag gctgaaaatc agccttgttt ttctatttga aaatgggatt ggcactaata
     6301 aatacgttgt ctgaagaaag tgatccgtct gcagaaactt cataagcatg ggataaacca
     6361 acgatacttt gattgttgta aagcaagttt gcgttatgag cagcgctatc tttccacata
     6421 accatagcat ttactgctcc tgtatctgaa aggatctcgt caacgtaaag gtagaaacta
     6481 ttgatatttt ctcctttagc accgtagcag cctaagcttt ctaagagatc ttggaaagta
     6541 ccgccgccag gtcggtaatg actgaagctt acttgtaatt cttttgcacg aatatctgca
     6601 gcttgttgaa cgatatcatt gaattgcatt ggggcaacgc cattttgttg acggtagcta
     6661 tttaaccaag caagttcttt ttgacgcaca tagttgttta aatcacttag tgagatattt
     6721 ttgtatttga aagtgatcgt tcgattttgg tcaacattat tgattacacg ttgtccattt
     6781 ttatcaacgg taacagatga atcgtcattt ctacacaaga agtagttttg atcggtcata
     6841 tttttccaag aagtagagaa actatctccc tcgtaaaccg tttgtgtttc agtagataaa
     6901 acagcgtttg tgtcaacgtt tatatggttc acagtgacgt tgtattgatg agtataggca
     6961 aaattgattc cttgattact tgtaacgttg tttaaagtca cagtgttgct agagctttct
     7021 ctaggatcaa gaacaaaatt agtgaacata ggtgcttcaa ttgtgatgct ttgtccataa
     7081 acgacgggat aactcttact gtaattatag agttcttgga tatttggatc agtatccgag
     7141 actttatttg gacgagcttt cacttcaact agaaagactt catcatagta aaaagtaaat
     7201 gtggtatttg tgttaatcgt ttgtgtttgt gtttcattcc catttacttg atactgtggt
     7261 gcttcaaagt tagtcaagtc ggttctagct tttgcagtgt aacttgtacc agcttttact
     7321 gagacatgtt cttcttttag cagcgttcca gtttgtcgat tcatatgacg gacagttacc
     7381 gcatattctg aaggtttagg tttgtgttgt aaccctttaa atgcagaatc aagactattt
     7441 ttagcattat ttacttgggt ttgagaagct ttagaatcgt ctagtacttt tttagcattg
     7501 gataaagcag tctgaaaatt attccaagat ccttctgtaa aatctccttt agcagttgat
     7561 tttacttggt tgtacaaggc ttgtaattcc gttttgttca cagtaggagc tggttttttc
     7621 tttaatccct tgtaagcaga atctaatgtg tttttggcac tatccacttg tttttgagtc
     7681 gcttttgagt catttaacac ttttttagca ttgtttaaag cggtttggaa tgcgttccag
     7741 gtattgtcgg tatagtcacc ttttttagtt cccttaacgc tcgtataaag agcttctaaa
     7801 gctgatttat tcactttggg tttagaggtg tcagcaccat accaaccaat accttcgtat
     7861 ttccagccat agtctttggt taaccattgt acttctttcc aacttgcagt gtagtgatgc
     7921 gaaccagagg taacacctgg aaggaaaagg cggtagatcg gtttattatt tttttcggca
     7981 gaacgccagg caggcccttc ataagtccaa ccgtaatttt tggtcaacca tttcacttca
     8041 tcccaactag ctgtgtaatg gtgatcacga aggcctttat tgtataaacg atagacaagg
     8101 tggccaccgc tagtcggtgc ttcccaagcg gggccttcat aattacccca cttaattttc
     8161 actaggtgat cacgttcttt tgtgctagag gtgtaaaaat gttcatggtt atttggatta
     8221 taaaggcgat acatgacgtt agttgaagca gcgtcagcag atccagcaag agcaactgtg
     8281 gcagcaaatg ttccaatagc tataacagtt tttgggttta ataatttcat gttttcaact
     8341 ccctattaaa tgattaaata ttttgtttac attcataata taccacggta taccgaggta
     8401 gtcaacggta taccgtggaa attttgaaat aaaaaagccg ccctatgaag agtgtaagga
     8461 tggctgttta tatgctatga agaaatggat tcggcgtatt tggttagttt ggcagcatta
     8521 tcaaatcgca ttttttcaat cgcacttttt ttctttatta aatccgcaat tgttgattct
     8581 gcgactgaag aatgtttgct aatttcatat gctgtcttgc cagtttcaaa tagtaaccat
     8641 tcgattttct ctttatcagc gtacattgca atgctccctc ttgatttttt ctttattata
     8701 gctctttttt gtaataaatc acaatatcaa gaggaaaagt ttctatagaa aggtgggaaa
     8761 ttatgagtat ccgttccccg tgtatcaaaa tgaggtttgt tgctactttc gttatttaat
     8821 atactaaaat aaaacaacga aagaaggaat agaatgaaaa agttaataat ggtatttact
     8881 tcgttgattg ccatgattgt tattttaggt ggatgtggta gtgaaaaaaa taagcaagat
     8941 agtcaagata atggcgtgtt aaaaccagga acagtgtgga aagaagaagt aggcggactt
     9001 gttcataatt taaaaattat agatgataca acatgggaat attccgaaag tgtttggcat
     9061 cctgaacaag tacaaataac agttgagaag ctaaaagact ataaaggatt agaaagatat
     9121 aaggtaatcg attcaggtgg agtagattta ttcataggta aaacagcctt aatagtttcc
     9181 tataacaaag acggtttaga aacgtttaaa tttattccaa caaacgaaaa agaacatcga
     9241 gataaaagtg aaatattaaa agaatacagt actaaatctg gatacaagtt acaaaagact
     9301 agcgagtgat tgctagtctt tttatttatc ctgaaggagg aatcgcagtg gcaaaagcta
     9361 aaacgccatt tgaaaaagcc gcgtctaaaa tcttagaacg tcgcggtgta tctgaaacac
     9421 agtggaaaca agaagtcctt gaaaaagcac aatttgacct attacgggga aaagacaagg
     9481 acttagaagg atatatctat caacgtgaac gtgaaaagct tgttttcgat gaaattatta
     9541 aacactaaaa ggagcgaaca attttatgtt tgaatcaatt acaatgaatt ttttaacaca
     9601 accgaaagta gtagctgcgg ttgatatttt tagagcatta caaggtgcag caactagttt
     9661 aaaaaacaat ctgatgattt ttgcggctgt agctgccgtt gcctccattg ttcttggggg
     9721 gattttcttc ttctttggcc gaagaggagc tgaaaacggg aagatgcacg taggttatgt
     9781 attgatgggg gtaggagtta tattagctgc cgcctctatt gtggtttcgt tatttggaat
     9841 gtttggacaa accccacagg gactataaaa aataaataga aggagtaaaa agtacgtggc
     9901 tactttttac tcctttttta tgaaaggaga atactcatgt actttacgat ttcaggcaat
     9961 accaaaaaag ataaagagtt aataaaaaaa gcaacgaaga aagtgggaaa gcgtactgcc
    10021 tttttaccga aaacaattaa atattctgtc attgaaacgg aagaacagct acatgtgtta
    10081 ggacaagtgt tagaacaaga gaatcaatct tctgttaaat tgactctcac gttacatgat
    10141 aaagaaggaa aaatagaaaa ggctggcaca gaatacgttt ttggcccact ggattatcaa
    10201 atttcagaag gtttttatcc tacattgatg gatttgatcc gccttgatgt gtttaaggaa
    10261 acggatgaaa ccatgagtgt ggaagagtct tatcgaaacg aaaaactgct acaagtttta
    10321 gaagaagagt tagagagcga ccaaagaaaa gtgagtcaag tgagtgaagt cgaagtaaat
    10381 gatgaagtac catgggaacg accaatcttt ccaaagaagg agtcaaaaga aaaagagaca
    10441 gtctatgttg acgaacaagc tgatattccg actcagaaat cgatatttga ggaagagaac
    10501 gatgtaatgg gtcaattgga ggtagatccc tttgaagaaa caatggagga tgaagagacg
    10561 gccgaagtag aagttgatct agttgaagct aatgaagagc caacatggac tgaaggggat
    10621 tctcaggaat atattgaacc acttatcagt gaagaagtag agaaaaacga aacagagata
    10681 caaaaggcaa ctgtatttga ttatattttg gatcaatatc cgccagagca tgaatggatg
    10741 aaagaaaaaa ataaattatt tttaaatgag ctttattcga aatatcaatt accccaaacc
    10801 tatcaagcat ttttagagag taaagaagaa ttaatctcac agggtaaagg cacactttta
    10861 gaacagttag agtctgttca atcaacaaat tggcatgagc ttgcacaaga agatttatta
    10921 gccacttttg aagaacgtca acaagaaaca gatcaagaaa taaatgagta tgcgacggaa
    10981 cagtcatcta attgggagag aacaaaagag gaactaaata aagaagaaca acgagtgatt
    11041 gaagaagaag tacaaaaaat taaagagcga tacgaaaaga aacgcgacaa tgcgtattcc
    11101 ttgtgtatag agaaaattaa taactttact aaaactaatc aagaagcttt atcgaaagaa
    11161 aaagaaaact tgttaaaaga acgtataaaa gaacgaaaag aagagttcta ccgaggttta
    11221 cgtacagata agctaaagat tagcaaagaa ttaaatgaac aactgaataa actttatcaa
    11281 acaacgactg aagttcttat ggaaagacac gggcagatcc agcaagaatt acaagcacaa
    11341 attccaaaat ggaaaaaaga gtatgaaatt caacaacaag aattgaaaga acaagaagaa
    11401 aaacaacgac aagaagaaaa agagcgcgaa caattagaac taaaaaaacg tgagcagcat
    11461 cttgcagaac aaaaagtggc gatagaaaaa caacggttag agcaagaaaa ggaaaaagaa
    11521 gcagctgctc gtaaggaacg tcaagaacaa atggatacat tgcgacaagc acagttgaat
    11581 tatctagcac aacctcaacc agtagctgct gttcaaacgc cagtgaacaa ccaagcaaat
    11641 ttaactcctt caccacagcg ttcctttaac ggatggatgg tgggatgtat tgcaagttta
    11701 agcttgcttc taggtggagg aacagtattt gcttttaatc atatcaatca atcttcacag
    11761 gcagattctg tagccacaac ggcgtcaatc aaggaagaag cccaggaaca agcaagaaaa
    11821 gagtatgaac aacaattgga agaattattg aatcgtacca atcagtcaac agcagagtca
    11881 agtatggaaa atagcaaaga gagtgatcct agtaaggcta aagaaggatc aagcgattca
    11941 actgtacact caaacacttc cgaaagttca gaatcaaatg aggtgaaata atgcgtatta
    12001 gagaaacaac gagacgaaaa gacaaacgat tcaatttaga aaagaatagg tatctttctc
    12061 atttctatcg ctatactcct tcaggagaag agctaccaga aaaacatgac aaacgtattt
    12121 ctcaaaagat tttttatagt ggcttagctt atcttccttt aaactatgtt gttacaagaa
    12181 taccagaagc atttcatcga tttgagatta ttccaggaag tttacaagga gtgaatattt
    12241 ttcagttaca agaaatgccg cagttattcc attttgattc aactggtttt tggaatcctt
    12301 tgaccccaac aacaagttta ggcacacgtt tagttgtttc tacgatttgt gttgttccag
    12361 ctgtacttat gggattgaaa atggacatgt ggtttcgtcc gctagcagat gggcaaaaag
    12421 gagataaccg attaaaaacg ttaaaggaaa tccaacaaat ttatccgcaa ataccagaat
    12481 caaagggaag ttttcctggt tatggcggaa taccaattac tcattataaa aaatactggt
    12541 atattcagac ggatacagta aatacttgta ttgttggtac ttcccgctca gggaaaggcc
    12601 agatagaagt tttagcaacc attgacaatt tgtcgcgtgc agaaagacaa agttcaatgg
    12661 ttgttaatga tccaaaaaca gaattatatg ttgctagtaa agatgaacta gaagctagag
    12721 ggtatgatgt gtatgccttt aatatcaccg atccactgca atcaatgagt gataatccat
    12781 tggcactcat tgtgaaatac tggaagcgag gagacataga caccgcaaca cagttaacca
    12841 atacttttac gaatacgatt tattatgatg caaatgcttc agacaataaa tactttaacg
    12901 aaaatgcgca gaaagctgtc aatgcgctta tttttgcgtt attggaacaa gcggatcaaa
    12961 caggggatta ctccaaatta acaccaaata atctcgttga actactttca gaaattggcg
    13021 gttttaatta ttatccagat cctgagaatg aattcaagca gctcaatgcc ttagatgagt
    13081 tcatgaatca gttaccccct ggaaatatag caaaaaaaca atatggggca acaaaaattg
    13141 gatctgataa agcgaaaggg aatatcttgt ctacggcaat tacaggattg aatccattta
    13201 ccctaacgaa aattgcgaag atgacttctc aatcgaccat tgattacaag tccattggtt
    13261 ttccgaagta tttggatctc aaattacatg aaagcctatt aaatcaacgc attgtcgtgg
    13321 aattttatca taagggaaag aaatttcacg aggaacgagt caaagttggc tatcgtggtt
    13381 tttgtgaaat caactttgat tgtcatttag agcagggaga tcatgtttgc ttaaaataca
    13441 atcatcaagg aaaacaatat gttgcttggt atgagttttc tcaaaaagta ttgaaagacg
    13501 aagagggaaa aatcgtttat cgaaaaaaac gaggagaaac tcatttgcca gaactagaaa
    13561 aagaagccgt tctaacatta gatgagaaga aaagtaacct attagtagag tctattcgga
    13621 tgcactatac cgataaacca atggctattt ttatgttaac gcctgattat gatccatcta
    13681 accatatcat tgttagcatc tttcttagtc agttgtataa agaattatct acgcaatgtg
    13741 ttaagactaa aggagataaa tgtcaccgta ggattcattt tattttagat gaatttggaa
    13801 atatgccacc gatggacaac atggaaggaa tcatgacggt tacggcaggt cgtaatatgt
    13861 t
//
LOCUS       DAYLZO010000074        13855 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.075, whole genome shotgun sequence.
ACCESSION   DAYLZO010000074 DAYLZO010000000
VERSION     DAYLZO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.075"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..577)
                     /locus_tag="ACGVJG_002000"
     CDS             complement(<1..577)
                     /locus_tag="ACGVJG_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFJ6557928.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
     gene            719..1651
                     /gene="pfkB"
                     /locus_tag="ACGVJG_002001"
     CDS             719..1651
                     /gene="pfkB"
                     /locus_tag="ACGVJG_002001"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFJ6557929.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1633..3096
                     /locus_tag="ACGVJG_002002"
     CDS             1633..3096
                     /locus_tag="ACGVJG_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFJ6557930.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            3093..3551
                     /locus_tag="ACGVJG_002003"
     CDS             3093..3551
                     /locus_tag="ACGVJG_002003"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFJ6557931.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3548..4522
                     /gene="lacD"
                     /locus_tag="ACGVJG_002004"
     CDS             3548..4522
                     /gene="lacD"
                     /locus_tag="ACGVJG_002004"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFJ6557932.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4963..5691
                     /locus_tag="ACGVJG_002005"
     CDS             4963..5691
                     /locus_tag="ACGVJG_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6557933.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5709..6905
                     /locus_tag="ACGVJG_002006"
     CDS             5709..6905
                     /locus_tag="ACGVJG_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFJ6557934.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6898..7893
                     /gene="lacD"
                     /locus_tag="ACGVJG_002007"
     CDS             6898..7893
                     /gene="lacD"
                     /locus_tag="ACGVJG_002007"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFJ6557935.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7890..8831
                     /locus_tag="ACGVJG_002008"
     CDS             7890..8831
                     /locus_tag="ACGVJG_002008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFJ6557936.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            9007..9486
                     /locus_tag="ACGVJG_002009"
                     /pseudo
     CDS             9007..9486
                     /locus_tag="ACGVJG_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            9499..10389
                     /locus_tag="ACGVJG_002010"
     CDS             9499..10389
                     /locus_tag="ACGVJG_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFJ6557937.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            10376..11185
                     /locus_tag="ACGVJG_002011"
     CDS             10376..11185
                     /locus_tag="ACGVJG_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFJ6557938.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11199..11600
                     /locus_tag="ACGVJG_002012"
     CDS             11199..11600
                     /locus_tag="ACGVJG_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFJ6557939.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="ACGVJG_002013"
     CDS             complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="ACGVJG_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFJ6557940.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(13156..13431)
                     /locus_tag="ACGVJG_002014"
     CDS             complement(13156..13431)
                     /locus_tag="ACGVJG_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFJ6557941.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            13539..>13855
                     /locus_tag="ACGVJG_002015"
     CDS             13539..>13855
                     /locus_tag="ACGVJG_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HFJ6557942.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTP"
BASE COUNT         4427 a         2284 c         2995 g         4149 t
ORIGIN      
        1 atttaaaaaa ttgaaaaata aagacataaa tcttattttt gatgttttta tttgttttct
       61 caccgttcaa agaaacaaaa ataacgctgt ctttcttagt tagtttccga ctgatgcttt
      121 tgataatttc tggatcagta tgcatctgac aattttttcc cgttaattga agttttacca
      181 gcatttcttg gccaatcatt tctgaaaatc ctcgggcaaa gacaaaaacc cggtcagcaa
      241 atttgattct ttgtattgca tcttcaatgt tcccgatttc caacatattg attgtacgta
      301 ccacttcttg ttcatttttt agaatggctc ggcggattcc tttgtctact ttatctaaaa
      361 atgaaaaatt gatgttcttg ttttctcctt cttttagatg atgcttaaac gaagtaaacc
      421 cttcatagcc tttctttttc attgtccgca caatggtggc tgttgagaca tttgcttgtt
      481 cacttagttt cacgatggaa tagttaggga tctctaggat gaggtgttcg ataaagttcc
      541 agaggtactg ctctgtttca cttaatttgt gattcatatt ctttccttct ttcaccaaaa
      601 gttagttaaa aaaagcacga cgagtttata aatgtaaagg ttttctttgt gttgggacga
      661 ttaagaaaac attttctatt tcgcgtattt cataatagaa tcaaggaggt cgttggttat
      721 gtctatctac acatgcacga tgaatttggc aattgatcta tttattgaaa cagatgagat
      781 gcatcctttt atggtaaacc gcacaaaaga ggatgacatt caggcaaacg gaaaaggggt
      841 gaatgtgtca ctagtattaa agatgctaga tttcccaagt acggcattag gtttttctgc
      901 tgggttcaca ggaaagtata tagaagacta tttacagcag aaacaaatcc aaacagagtt
      961 tattgaagtg cctggtatga cgcgtatcaa tgtatttaca caagtcaatc aaacgggaga
     1021 agaatacaaa ttagtgaatc aaggacctga aataccgcag acagctgttc ataatttttt
     1081 aaatcaaata agagcattac aagcagggga ttatctttgt gtttctggga gtttaccaag
     1141 agggctgtct ccaaaggttt taatagaaat cagtcggatt tgtttgatta ttgatagtag
     1201 tgatcaagaa gtcatggatt gtttacccta tcgaccgttt ttgctgaaac caaatgagga
     1261 agaactttct tcttggttcg gacgaaaaat ggttacagat gaagattact ttgtttatgg
     1321 acaacgacta gtagagtcag gcgcagaaaa tgttttgtta tccctcggtg agaaaggagc
     1381 gattcttttt acgaaagaca gggtgattcg aggaaactcg ccaaaaggaa aagttgtcaa
     1441 tacagcatgt gcaggtgatg ccatgttagg cactttttta gcaggataca tgaatcgacg
     1501 atcgcttgat gaaacgttac gtaaaagcat agcagcaggc agctctactg cttttcgtaa
     1561 aggactgact gattttttgg atgtggagga attaagtaaa caaatcaaaa tcaaggaaga
     1621 gaggttcgaa agatgggaaa atatcaacta attgcagcaa ccggttgtcc gacaggaatt
     1681 gcgcacacct acatggcgca agaggcatta gagcaagcag ctaggaaaaa aggcatcacg
     1741 atcaaagtgg agacccatgg acaaattggg atcgagaatg aattaacggc tgaagaaatc
     1801 aaacaggcag atgctgtcat tattgccgca gacaaagatg tccattcaga aagatttgtg
     1861 ggaaaacgag tcattgatgt ttctgtcagt acaggaatca aggatgcaca acggctgatt
     1921 gacgaagcat tggcaggaaa aggtactgtc ttagcagata atgaagctgc tgatacttta
     1981 gaaccagaag cgataagtgg tgctaatttt ggtcgtagca tctataaaaa tttgatgaac
     2041 ggtgtctctc atatgttgcc tttcgtggta gctggcggag tattgattgc tatttcattt
     2101 gccgtttggg gaatctattc ttctgatcca gatcatgcac aatacaatgc tacagcggca
     2161 atggtaaaag cagttgacga tgcagcaatg ggaatgatgg ttccagtttt agcagcttat
     2221 attgcagaag ggatcgcgaa acgtccaggg ttagtcgttg gatttgttgg tggaatcgta
     2281 gccatgaatg gcggtactgg atttttagga ggtattttag ctgggttctt agcaggttat
     2341 tttatcttat ttttgcaaaa gctactgata attttaccta aacaactaga tggcttgaag
     2401 gcaatcgtct tatatccagt tattggtgta gcatttattg gcatcgtgat gtctttgctg
     2461 gcaggtccaa tgaaggctat aaatgaagga atgatgagct ttttagctgg atttgaatat
     2521 tcgaatccac ttgttcttgg cgtgattgtt ggctgtatgt gtgcgtttga tatgggagga
     2581 ccggttaata aggcggcata tgtaacagga acagctcttt tgacacaagg aaacacgact
     2641 tttatggctg gtgcgtcagc agcttgcatt gcaccaccgt tgattactgg ttttgctact
     2701 ttattgtttg gtaaatattt tgatactaat gatcgtaatg caggtttggt taattttatt
     2761 ttaggatcaa ctcatattac ggaaggagct attccgtttg ctgcgaaaga cccgatccga
     2821 gtattaccaa cgatgatgtt aggctcatca attgctgctg tcttgaccta tctgtttggt
     2881 gtccaagtac cagccccaca tggcggtttt ttagtgttac ctgtagtgac gggaaaaatc
     2941 caatgggtta cagcgattct tattggttcg atcgttggtg gagtgatact aggtttgatc
     3001 caaaagaaaa gagtaactga taaagacaag aagagggata ataaagcaac aaatcgtaca
     3061 gttatcgaag aaggaagagc aggaggttta tcatgacaat cattcatgaa gagaacattt
     3121 ttttacaaca gccattagat aatcaagaag aagcatttaa gtttttagca aagcaaacat
     3181 tctgtttagg aatcgctcat gacgaaaaag aagtatatca aaaacttctg gaaagagaaa
     3241 aagaaggtac tacaggaata atgtcaggtt ttgctatccc tcatgcgaag tctcaaacaa
     3301 tcaatgaacc tagtttgatt atcgtcacac tgaaaaaagg aattgaatgg aacagtttag
     3361 atagacagtc agttgatttt atttttgcgt tatttatccc agattcagaa gcaggaacga
     3421 ctcatttgaa attattgtct ctcgttgcac gatcattgat gcgaaaagag gttacagaac
     3481 agttgagagc tgcaaaatca aaaatagaaa tagctacaat attaactaaa gaaataggtg
     3541 aagatatatg aagaaaatga gtatagaaaa acaggaacgt ttgaagcgat tgacggatga
     3601 agctggaatt attagcgcgc tagctatcga ccaacgtggt gctttgaaga aaatgatcaa
     3661 ccaatataaa gaagctacat cagaagatat catccaattt aaacagatgg tttctcagta
     3721 cctgactccg tatgcttccg ctattttact cgatccagaa tatggtctgc ctgcagcagc
     3781 gcttcgagag aaagaatgtg gtttgttgct agcttatgaa aaaaccggct atgatgcgaa
     3841 aatgccaggc cgactaccag atttactgaa tagttggtca gtgaaaaggt taaaagaagt
     3901 gggtgcggat gcttgtaaat ttttattgta ctatgacgtt gatgaaccag tagagattaa
     3961 tgtgcaaaaa caaatagtta tagaacggat tggttcagaa tgcgaagcag aaggcatacc
     4021 tttcttttta gagctccttt cttacgatgc aactattggc gatccgtctt ctcttgacta
     4081 tgcaaaagta aaaccgcata aagtgattga agccatgcga gaattttcaa aagaacgtta
     4141 tcaagtggat gtattgaaag tagaagtacc agtgaatatg agatttgtgg aaggatatgg
     4201 gcaagatact tgttatagtc aagcagaggc atccgcgtat ttctatgaac aatcgaaagt
     4261 gactgaacaa ccgtttattt ttttaagtgc gggagtcagc gctgcactat ttcaagatac
     4321 attgcgtttt gcaaaacaat caggttcgac attcaatggt gtcttatgtg ggcgagcaac
     4381 atgggcgaag ggagtagcac cttttgttac cgaaggcgaa gaaaaagcga gtagttggat
     4441 gttagaagaa ggaaaacaaa atattaaaga attaagtaca ctattagaag aaacagcaac
     4501 ttctatttat gttaacaagt aagagaaagg attgtctgag tgacagattc aaaaaataat
     4561 tagttattta aaaaaatgtt tgtcaaattt tatagaaaaa aagtataaaa ctgcaaaagt
     4621 tatctaaaac taataataac atttgtcatt aataagctat ttttagttgt tgtaaatgat
     4681 attggaagta ataaaacgat agttgtagga atatcatagg gattaatgtg gatcacgctg
     4741 ctcgataagt tagaccattt tgacggatcg cttatgtggt tcgtcttttt ttctgttctt
     4801 ttattggtaa ctggtatttt tgaggtatct ttgtctattc aagatataaa gctttaaaga
     4861 gctgttataa tagctttttt ggtctaaaaa acctttgaca aatgattaat aattcactat
     4921 tcttgtgttg tggttataac cagtaaagaa gaggtgattt ttatggagag ctcaaataaa
     4981 ggaaaatcat tgtattatca actagttgat ttattgaagg atcgtatcga aacacagatg
     5041 actccgcatg ataagctgtt atctgaaaga gaactttgtc aacactatgg ggtgagtcgt
     5101 accaccgtac gtctggcttt acaggaattg gaaaatcaag gttatattta ccgaagacat
     5161 ggaaaaggta cctatgtctc tgatttgtct gataagtcat cgaacttagc tggagcatat
     5221 agctttacag aagaaatgcg cgaaataggg cgcgtcccta agacggtcat tttagaattt
     5281 agacaaatgg aagctaataa gagaattgct aagaatttga acatccattt aggtgaaact
     5341 gtatttaaac ttaaaagatt gcgtttggca gatgaagagc cattgatggt tgagcgttct
     5401 tatctaccgg cgaaactatt tatgggactt gatttgccgt tacttgaaag taaaccgttg
     5461 tatgatgtgt tttcacagga ttatgaacaa caagttcaat tagctgatga agagttttat
     5521 gcaagtatcg tgcaaaaaaa agatgctgtt gtgctacgaa tcgctgatgg cgctcccgtc
     5581 ttacatctga cgaggataac ctataacgtg aaaaatgaga ttatcgaata tacagatagt
     5641 gtagcaagag cggaccagtt tcattataaa attcggcata tccgaaaatg attgagaggg
     5701 agaacaacat gtttgagcta tcgaagaaca aattaaaaca aataggtgca gcaatcacga
     5761 cacaagaaat aaaacaacaa ccagatctct ggaaagaaac aatcgataga ttgatgcaaa
     5821 gccaagaaaa tttagctcta ttttttgatc aaataaaaaa agaagcaaac ggaagaagaa
     5881 tacgtgttat ttttacgggt gcaggaacgt ctcaatatat tggggatacg ctcttaccct
     5941 atctaaatga acatggagac aatcagtttt ttatcttcca cagttttggc acgacagata
     6001 ttgtagcttc accaaaagaa tttttgtttc caaatgaacc gactttatta gtttcatttg
     6061 ctcgcagtgg caacagtcca gaaagtgtag cggctgtgca aattgcaaat gaacaagttg
     6121 aaacattgtt tcatttgacg attacttgtg ctaaagaggg gatgttggca aaaaacgcac
     6181 aggatgataa acagaattat ctgttattga tgccagagag agcaaacgat gcaggttttg
     6241 ctatgacagg aagttttacg tgtatgttat tgagtgcttt atggatcttt gatcaaacag
     6301 caatggagaa aaaagaaaga tatcttgaac aattagttaa aatcggtcaa gatcttcttt
     6361 caagagatca agaaattcaa aagttattac ctgatttttt tgaacggatc gcttatctgg
     6421 gttcaggaag tttggcaggt gcgacaagag aagcacaatt aaaaatttta gaattaacag
     6481 caggtaaagt tgctacaatt tttgattctt cgatggggtt tcgtcatgga ccaaagtctt
     6541 ttttaaatag cgagacactc gttgtaggtt ttgtgagcaa tcaaccatat acgcgaaatt
     6601 atgacttaga tattttagag gaaataaaag aagatcgggt cactgccaat gtcattgcga
     6661 ttgcgcaacc gggtactatt gatttctcag gtatgactgt gctattggaa gactcggtga
     6721 gtaaattgcc cgatgcctat cttgcattag cgatgattat gactgcacaa atcattgcac
     6781 tctatacttc aatcaaagta gctaataaac cagatacacc atctccaaca ggcacggtca
     6841 atcgagtagt caagggcgtc attattcata agtatgaaac cgaaggagaa atgacagatg
     6901 aatgaagaaa aaaatagggc aatggagaga ctatcaacaa aagatgggat tattagtgca
     6961 ttagcgatcg atcagcgtgg tgctctaaaa aaaatgatca aatcgttggg aaaagagcca
     7021 agtgaagacc aaatcagtga attcaaagaa ctagtatcag ctgagctgac accctatgct
     7081 tctgctattt tattggatcc ggaatatgga ttgccagcag caaaagtcag agcaaaagag
     7141 acggggttat tgttagctta tgagaaaacc ggctatgacg caactacgcc tggtcgtcta
     7201 cctgatttat tagcggattg gtcttctctc agattgaaag aacagcaagc ggatgcagta
     7261 aaattcttac tttactacga tgttgatgaa gcacccgaaa tcaatcattt gaagcatgtg
     7321 ttcatcgaac gtttaggtag tgagtgcgaa ggagaaaatc gtcccttcta tttggagtta
     7381 gtttcttatg acgcacaaat aagcgatagt tcttctccag agtatgcgaa agtcaagccc
     7441 cacaaagtta ttgagatgat gaaagaattt tctgatccaa aatataaagt tgatgtgttg
     7501 aaagtagaag taccagtcaa tatgaattat gtgaatggct attccacagg tgaatttatt
     7561 cacagcaaag aagaggctgc tgccttcttt aaggagcaaa gtgaagcgac tccattacca
     7621 ttcatttttt tgagtggggg tgtttcaaca gaattatttc aacaaacact gatttttgct
     7681 catgaatcgg gttccacctt caatggtgta ttgtgtggac gtgcgacatg gaaagatggg
     7741 gtcaaagcat acgtcgaaaa tggaaaagac ggagctattc attggttaag aacaaatggg
     7801 agagaaaaca tcgagacttt aaataaagta ataaatgaaa ccgctacccc ttggttcaat
     7861 aaaacaaaaa gcaaaaaggt gaagatgaaa tgattacgac agttaccttg aatccttcca
     7921 tcgatatcgc gtatacaatt gaagaattaa acctcgacaa agtcaatcga gttgaaacag
     7981 tcaataaaac agcgggagga aaaggagtga atgtggcgag ggttctaaat ttattaggag
     8041 aagaggtgat agctactggt ttaataggtg gccagcacgg tgaatatcta aagaaccagc
     8101 tagatcagtt agggatcatg catcagtttg ttccaattaa aggagagaca agggattcta
     8161 ttgctatcct gcatagttcg agacaaacag agcttttgga acgaggcccc ttcataacaa
     8221 agaaagaaca acagacattt accgatcatt tcgagcagct cctcgaaata tcagatatta
     8281 tgacgatctc aggtagttta ccccaaggat gccctcttga ttactataag caattggttg
     8341 ctttagctga taaaaaaggg aaagacatat tactagatac ttctggtaaa tttttgcttg
     8401 aagtattgga aggtaaggag aagccactac ttatcaagcc caatctggaa gaattaggag
     8461 aactgattgg ccaacgtatc tgttcttcga ttcaattaga aaaaattcag tccttattag
     8521 ctcatccaat atttgaagga gtgccatggg ttgttgtttc tttaggagag agaggtgctg
     8581 tggcaaaagt tgctcagtgt ttttacgaag tcattattcc taaaatatca gttgttaatc
     8641 ctgttggttc aggtgacgcg acccttgcag gatcggccca tgcgttatca aaaaggcaat
     8701 caatcgaaga gattctcgca ttttctatgg cttgtggtat ggcaaacgcg caacaagaga
     8761 aaactggctg gatcgaccct atgaaagtta cgatgttgat gaaacacatt caagttaaga
     8821 aaatcaaatg aacttaaata taaaagaaag cgttttcttt agttgggaag gaatttgttc
     8881 ttagcttaaa aatgaaatat aagaaagaaa aaatgaaaac gttatctttg aaacagaagg
     8941 gatatttgat gatgtcacag ttttaattga acaataaata gaaaaaatag ataaggagaa
     9001 tgcatgatgg caattattgg tgtaagaatc gatggacgtt tgatacatgg ataagtagca
     9061 aatctttgga caacgaaact gaatattagt cgaatcatgg tcgtggatga tgaggtagcc
     9121 acaaacacaa ttgaaaaaag tggattgaag ttagcgactc cagcaggggt aaaattaagc
     9181 attttgccaa tcgaaaaagc agcaacaaat attttagctg gcaaatatga ctctcaacga
     9241 gtattgatcg tagcaaaaaa gccagatcga atcttatctt tagtagaaaa aggagttcct
     9301 atcaaagaag taaatgtggg gaatatgtca caaacgaatg aaacacggtc cattacaaaa
     9361 tcaatcaatg tggtagatgc agatatcgaa gtatttaaaa aattagatca gcttggtata
     9421 catttaattg ctcaaatggt accaaacgat aaagcagaag actttatgaa tcttttaatc
     9481 aaataggagg aatgatccat ggatattcaa tggtggcaga tcttattatt aacattatat
     9541 gcaggttatc aaattttaga tgaattacag atctattctt caatgagtgc gccagtgttt
     9601 gcagggttat atgcaggttt agtaatgggt gatttaaacg caggtttgat tattggtggc
     9661 agtatgcagt tgacagtttt aggagtgggg acgtttggag gcgcatcaaa aatagatgcc
     9721 aactctggga cgattttagc aactgccttc tcgatttctc ttggaatgaa tccagaacaa
     9781 gcgattgctg ctattgcagt gcctgtagca agcttaatga tccaattgga tattttagct
     9841 agattcgcta acacatactt tgcccatcgt attgaccgtc acgtagaaga catgaattat
     9901 caagcaatcg aacgcaattt cttaatggga gctttacctt ggtcgctctc tcgtatgatt
     9961 cctgtatttt tagctttagc atttggtggt ggattagttg aaaatgttgt ttctgtttta
    10021 aacgggaatc taaaatggct aggcgatgga ctttcagtag caggagctgt tttaccagct
    10081 gttgggtttg ccattcttct gcgctattta ccagtaaaaa aacatctatc ttatcttatt
    10141 ttaggattta cgatcacggc attattagca acgttattct ctaatgttca attattagga
    10201 acaagtgtgg caagcgttgt taaagatctt ccggcaactt tcaattcgct gccgatgctt
    10261 gctgtagcat tgattggtct agcactagca atgatcagtt ataagaatga tcaaaacaaa
    10321 gaccatcaac cattagctag tgagccatca cattctgttg agggggaaat cgaagatgac
    10381 gaaatctaat tacaaattaa caaaagaaga ttttaagcaa atcaatcgaa gaagtttgtt
    10441 cacttttcaa ttaggttgga actatgagag aatgcagggt tctggctatt tatatactat
    10501 cttgccacaa ctaagaaaaa tatatggaga caatacacca gaattaaaag agatgatgaa
    10561 aacacatacg caatttttta atacttctaa tttttttaat acaatcatta ctgggattga
    10621 tcttgcaatg gaagaaaaag agggaattgc ttcaaaggaa accgtatcgg ggctgaaagc
    10681 tggattgatg ggttcctttg cagcgattgg agattccgta tttgctgctt tgattccaac
    10741 gatttttggg gctttggcgg cgaatatggc aactgatgga aatccgatag gtgtgtttat
    10801 atggattttt gctcagatcg ttgtcatggt ttttcgttgg aagcaacttg aatttgcata
    10861 caaagaagga atttccttag ttactactat gcaatctcgc ttaactgcat taacggacgc
    10921 agccacttta ttaggggtat tcatggtagg ggcattagtt gcgacaatga tcaatgttaa
    10981 attttcatgg gcaccaacga ttggcgatgt gacattgaat atgcaaaact atatcgatat
    11041 gatcttacct aaaattttac ctgctgggat cgtcggtggt gtttattggt tattaggaaa
    11101 gaaaaacatg actcctactc gagcgatttt tatcgtatta attgtgtgtg ttgctttatc
    11161 tgcattgggt gtgatttcga aataagggag agcgaaaaat gagtagacaa ttggtattga
    11221 ttagtcacgg aaaattttgt gaggaattga aaaaaagtac agaaatgatc atggggcctc
    11281 aagaggatat ctacacgtta cctttattac cagaagatgg cactgaaaca tacaaacaaa
    11341 agtttctcac tgtcacagat tcgttaaatg attttgtggt actatgcgat cttttaggtg
    11401 gtacacctgc aaatatcatt tctaaactaa taatggaagg acaacagatt actctttatg
    11461 ctgggatgaa tcttccgatg gtcattgaat ttatcaactc acaaatgatt ggttcagagc
    11521 ccgagtttgt tcgtgcagga tgtgaaagca ttgtctctgt caacgaatta attaatggaa
    11581 tagcagaaga ggaagagtaa caaaaaagat gagggcttag ctctcatctt ttttgtccta
    11641 tgtttttatt tccgcttctg ctgctttcct gcgttgcggc ctttgttgtt tgtactgttt
    11701 tttggttgtt tgatttgttt tgttgcttgt ttgttgttag ctggctttgc tgattctgtc
    11761 acgtcttttc ttggagtgac gacttgtttt ggcgggtttt gtttcaactc ttctgccact
    11821 tgtgctttca aacgtggttt catcataacg tttgtgatga atgtttgcag acagctgaac
    11881 acaccaccga ctacccagta aagtgtaaca ccggctggcg cactgatcga catgaagacg
    11941 atcatagcag gagagacgat tagcatcgta cgcatcgtct ttttctgttc ttcaggaata
    12001 ccgatcgttg agatgtatcc ttgaagcaga taggcgaccc ctgcaatgac aacaagtacc
    12061 atacttggtg agcctaaatc gatgccgtag aaagatgatt cccgaatacc ttctgtataa
    12121 cgtgccgtaa agtacaaggc agagaagatc ggcatttgga tcaatagagg cagacagccg
    12181 attccgccgg tcatactcaa tccgttttcg cggtatactt gctgcatttc tgcttgtgct
    12241 gccatttgtt cttcacgtgt tgttgcagct ttcaattttt gttgggctgc ttcaacttgt
    12301 ggttttaacg cttgcatttt ttcagattgg atcattgttt ttttcgattg actaatacct
    12361 agtggcaaga tgatgatacg gacgatcaca gtcattgcaa taactgccca gccataagac
    12421 caattgaaat tctcaactag atatgtaatc gcttggccca taggatggac gagtactcga
    12481 taaacgattc ccgaagtgtc agggtttccg tttgaatcca tccgcacaca gccagataaa
    12541 aagagcagga tactgacaag tccggatccc aataaccagt ttttccattt attcatagaa
    12601 tacttcctct tctataattt ttacacaaac ttaactatac taaaaaagcg catggaattc
    12661 aatcaacttc atagaaattt catataaata ttcgttcttt tacgtagcga aaaattttga
    12721 aaaaaagaca aaaaatcaag aaagaaatac ttggaaagaa aagagtgatt ccaagtgttt
    12781 ctttcttatt taaaaaacta aaaacttttt tgttctgtct ggaaaatatg ggttaagcaa
    12841 atttattgca agttatagat cagctttttt actacctatc tttaatactt gaggcaaagc
    12901 gtctcaaaaa caaccttatc gacggtgttc aaaagtcagt ttctcggtat taagtcaaat
    12961 tttatgcaaa aatgtggaaa gccattttca caaaattctc cttactactc aaaactaagc
    13021 gactttctca caaccttatt gacggtgttc aaaagcttgc atctacggca ttaagtccag
    13081 cctgttgaaa aatatggaga gctatttttc acaacctgtc cttaatgctt gatgcagacc
    13141 agctttttca caaccttatc ccaccacatc aaatttcttt cgttcctcaa tcatcgggtc
    13201 ttcttggaca tccacatact gcactcgtcc acttgggctc ggagatttct tgacttcttc
    13261 aataaatttc tggatgatgt cgtcattgcc gattgcttcg atcgtgactg agccgtcatc
    13321 ttcgtttttt accgttccag tcacgcctag ttgatcagcg accatcttgg tcatgtagcg
    13381 aaatcctacg ccttggacgc gtccttggac gttcatttta actttgcgca tgttcgtcac
    13441 ctttctttcc tattttataa attaatgata ccgatttttt taggatgaaa caagctcaag
    13501 cacctattcc atgatataat catggggagg tgaggaatgt gaaagagatc caatcagcaa
    13561 aaaatccaag aatcaaagaa ttaaaaaaac tacataaaaa gaaatatcgg gaagaaaagg
    13621 aagaatatct tttggaaggg ttccatctga ttgaagaagc tgcgtcgtct gatgcagaaa
    13681 taaaagaact ttttgtgaca cagcgaggca tgaaagaatg gggagactgg ctggccgcaa
    13741 acaaagagat ttctactttt ctagtttcgg atgaggtcat ggatgctgta tcggatctac
    13801 ctacaccaca aggaatctta gcagtgatgg cgttaacggc tgaaggcacg ccaga
//
LOCUS       DAYLZO010000075        13707 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.076, whole genome shotgun sequence.
ACCESSION   DAYLZO010000075 DAYLZO010000000
VERSION     DAYLZO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.076"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..853)
                     /gene="gnd"
                     /locus_tag="ACGVJG_002016"
     CDS             complement(<1..853)
                     /gene="gnd"
                     /locus_tag="ACGVJG_002016"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFJ6557943.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVS"
     gene            1017..1865
                     /locus_tag="ACGVJG_002017"
     CDS             1017..1865
                     /locus_tag="ACGVJG_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFJ6557944.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            2511..2717
                     /locus_tag="ACGVJG_002018"
     CDS             2511..2717
                     /locus_tag="ACGVJG_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFJ6557945.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(2919..3920)
                     /locus_tag="ACGVJG_002019"
     CDS             complement(2919..3920)
                     /locus_tag="ACGVJG_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFJ6557946.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(3925..5838)
                     /locus_tag="ACGVJG_002020"
     CDS             complement(3925..5838)
                     /locus_tag="ACGVJG_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFJ6557947.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(6006..6512)
                     /locus_tag="ACGVJG_002021"
     CDS             complement(6006..6512)
                     /locus_tag="ACGVJG_002021"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFJ6557948.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6672..7112
                     /locus_tag="ACGVJG_002022"
     CDS             6672..7112
                     /locus_tag="ACGVJG_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFJ6557949.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(7138..8253)
                     /locus_tag="ACGVJG_002023"
     CDS             complement(7138..8253)
                     /locus_tag="ACGVJG_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557950.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(8298..8654)
                     /locus_tag="ACGVJG_002024"
     CDS             complement(8298..8654)
                     /locus_tag="ACGVJG_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557951.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8952..9926)
                     /gene="sgrA"
                     /locus_tag="ACGVJG_002025"
     CDS             complement(8952..9926)
                     /gene="sgrA"
                     /locus_tag="ACGVJG_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFJ6557952.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(10122..10961)
                     /locus_tag="ACGVJG_002026"
     CDS             complement(10122..10961)
                     /locus_tag="ACGVJG_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFJ6557953.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            11146..12033
                     /locus_tag="ACGVJG_002027"
     CDS             11146..12033
                     /locus_tag="ACGVJG_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFJ6557954.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(12627..13321)
                     /locus_tag="ACGVJG_002028"
                     /pseudo
     CDS             complement(12627..13321)
                     /locus_tag="ACGVJG_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(13305..13703)
                     /locus_tag="ACGVJG_002029"
     CDS             complement(13305..13703)
                     /locus_tag="ACGVJG_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFJ6557955.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         4118 a         2721 c         2072 g         4796 t
ORIGIN      
        1 ctgaaactac ttttgctgaa aaactatctt cttcctgtga aagattcctc atgaacaaac
       61 tagttgcaat tactggaact ggaacagcca tttctagcgc tgtttcaacc gtccattttg
      121 cttcacctga tgcagaaacg attccccgat aattacttaa attgggatgt tcgtttaact
      181 gatcttccat gatttccatc agccagccgc gaatcaccga accatgattc caaacacgtg
      241 cgacttgttc caagtcaaaa gcatactcac ttttttgtac aagttggaat ccttcaccga
      301 ttgcttgcat cattccatat tcgattccat tatggatcat tttcaagaag tgtccgcttc
      361 cggatgcacc agcatacaga tatcctcttt caattgctac atctttgaat aattgttcca
      421 cggatagaaa agtttcttta tctcctccaa tcattaagca ggcttgatgt cgtgctcctt
      481 cgattcctcc agaagtccca caatccaagt aaccgatttc atttttctga aactcttctg
      541 ctcgacggat tgaatcttta taataagagt ttccgccttc aataataatg tctcctggtc
      601 cgagttttgg aaaaagtttt cttaacatgt tttcggttat ctctccagca ggaagcatta
      661 accagatgat ttttttctgc tcaaaagtag ctagaaattc agagatctct tgataagcag
      721 gaatgtcatt ttctcttgcc tgactaacag ctgtacttga aatgtcgagt ccttgtactt
      781 tgtgatggtg ctcttttaag tttaaagcta aattcaatcc catttttccc aaaccaatta
      841 caccaatttc catgatagcg cctccatttc ttataccctc attatacaaa aaaatttttt
      901 tgtaaataga ttttacaaaa atattttttg tttgattttt tcctactctc cttgtatctt
      961 taacattaat gagaacggaa aaaatgataa tcttattatg aaaatgagga attctcatga
     1021 gcgtctccat aagactacaa atcaaaacaa tgtacaacga cttcagccca aaagaacagg
     1081 ctatcgccga ttatatttta gaaaatccaa gcaaagtaag tcatagtcct attagcaatt
     1141 tagcaagtga actgggcatt gctgactcta ctttttttca atttacaaaa aaacttggtt
     1201 ataatggatt taaagatttt aaaatggcca tgttgatcca agaaaatgat ctatctgcta
     1261 tttctatcca cgaaaatatt cagaaaacag atactgagct cactatggcg caaaaggtgt
     1321 tcgagtctag tatttcaact ttaaatgaca caaaaaagtt gttacaacaa aaggatctaa
     1381 aaaaagcggc tactattatc aatgaatcta aacgtgtcta ttttttcggt gttggcggtt
     1441 cagagattgt agcaactgac gcttatcata aatttttgcg ttcccctatc gcaacttctc
     1501 acagcacgga ttaccatatt caattgatgg aagcttcttt attgacggaa aaagactgtg
     1561 ctgttttgat ttctcacaca gggcaatcaa aagaaacaat ccatattgct gaaaccgtaa
     1621 gaaaaactgg agcaaaaaca atcgttatta ctagccaagc taattcttcc cttgctaaac
     1681 ttggcgatgt cgtttttatt tctatttctg aagaaacaga attccgatct gaagctttag
     1741 cttcccgtat ttctcaacta agcattttgg actctctata tgtgattttg atgttttaca
     1801 acgaaaaaga atcacgtcgt tctatcgcta aagttcgaca tactatttct gaaatcaaag
     1861 aatagcacca taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca ctaccaaata
     1921 tttggtagtg gcttttttgg ttctgcaagg gtggtacaga acatcaatag tcggtgggtt
     1981 agaacgtatg tcatcgcatc tttggagaag gttgtattgg tattattcga gttacttgtt
     2041 agatgcgata acaaagttag tatacagtag taaaaaattt atcttttctt ttttttatta
     2101 tttttgtttc ttattttcta aaaaattaaa aaaaattttg gtgaattaag tatttccttg
     2161 ataacactgg cttttggcct cattttaggt tatcatttgt aattttattt cacagttgat
     2221 atgattgtta tgtaaaataa aaaagtttag gatacatcga atttttgggg aaattctttt
     2281 gggggtatcc taaatctttt ctattatatt cccatatttt ctattattgg tctagacgta
     2341 tgatggtaaa ctttgtacct atctgattgc tgaatcatta attgcttatc attagccatc
     2401 tttgtttcaa gttcgctaga tgatcatttt attcacatcc taacaaactt gtcacattta
     2461 tagattctta atctatttat catacaatta taaaaaagga ggtgctgctt atgaaagcta
     2521 tccatatttc tttgcttttt ttcgctagtg cattgatttt atttgctatt catctttgtt
     2581 cccagcctta tatcaataac aacggaatat tagtggaacc agcattcttt tgcttaccac
     2641 tcggcttttt gtcccttctt gcctccgcag gcttcggatt tgttgctttt tttaaacgat
     2701 caaaacaaca aatctgactt ataaaattta cgtaaaagag atcgtgacaa taatcgtgtg
     2761 atattcccaa atttcgaata aaaggtgccc cttgccatct ctaactcctc agtcacaagt
     2821 tacaatattc ataaaccatg aaaagcttgt ttattgaaat tgctccttgt gacttggagc
     2881 taagtgacag attacaacct cttttgcatc ataagacttt actccttgaa aaacattact
     2941 aacttttgat atgtttcttg cgcttgttta ttgctgaaat caaactgata ttcatggttc
     3001 aaaacagcat ttttctccgc ataatctact tttgtgactt tcacccccgc tttctcaaat
     3061 acagaagtta cttcttccat ttgtttcgta aacgtatttg tatttccatc cgttaagaat
     3121 gtgttttgcg tccaacgatc cgcatgtctg accaaggcta aacttttgac cttctctgat
     3181 tgttcaaagt ccttattatc aaagtaagcc catccacatt tttcataaag aaagctagag
     3241 attttatcac ttacttgagt aagtgcagaa ggatccaaca gaccagacaa agaaataaac
     3301 ttcgtaatct gcttgttctc caaagtcgga tcaagatgat cagctttttt aatttcttca
     3361 ttttgaagtg ctaacaaata ttctccacta atttgtgcac ctgccgaatt tccaccaatc
     3421 gaaatattcg accaatctgc ttgatctttt atctcttttt tagctgcttg aatggcttcg
     3481 ttcaattgga taactggtgt aggatgtttt gcttccggtg atcgttcata atctatattt
     3541 acgatgattt gctgcgtgtc tgaacataga tattccaaat aatcacgaca atcattttta
     3601 tcccctccta catatgcgcc tccatgcatc cagaaaagaa tcggtaattt tttcttttcc
     3661 actttcggtg tgtagatatc catattatta tttttgtact ttgaaggata ggatatttct
     3721 ttttttatat cgatttgttc gattttttgc tggaaatcat ctggtcgggc atatacttgt
     3781 tctttttcac ttgtgtcaaa ttgttttcta acaagcaagc taatcggctt tggtgtgatt
     3841 tgattaatca aaaatacagt gactaaaata actacgatga taactggtac tgcaatcact
     3901 attcgcttga accatttcat tcatttaccc tctctttctt cgccaaatcc atgtgataat
     3961 cgtgctaaca gataaaaatc cacctgaata aatgagaagc gaatgtttct tttctccagt
     4021 tttcggcaac attctttttt ttgcatctgt ctttttttgt actttcactg tcatctgaat
     4081 cggttgcgta tcatcacttg attgatcctc tcgaggttca ttctttccct tattgtctgc
     4141 attctctgtc ggatcttgct tttttggtgg agctactggg atttttttgt aatagaaaat
     4201 aatagttggt atcgtctcat taaaaatccc ttcagtcggt ccttcaacat ttgctaattc
     4261 gtatccttcg aatgtctttt gctctgtcat ataattttct ccataagctc cgtaaagctg
     4321 gatttcgtcc tggtctttga gtagactttt attgttttca tccacatact ttacgtttat
     4381 ctgatcatgg aacactactt tcaaaaagtc cgttacatgt ttcgtgtagg cagcatggtc
     4441 aacttcctgt gcctttccat gccctgctcc ttctactaat aaaagatctt tataccctcc
     4501 tgataactca tataattttt gcgcgttaga cacaggtact acatcatctg ctgttccatg
     4561 gataatcaat ttaggcaatg aatctgacat tccaatcgta ggtagagcag cttttgtatc
     4621 caaatgcatc tcctgatcaa aatagtattg tttcaaaagg cccataaata aatcttcatg
     4681 ctcttgatcc ccaatgatgt ctaactgatt ttttatggca tccggtagtg cgggggctac
     4741 tgtctggctg tacgtaaaac gtagttgttc atcaattgat gcaaatccgc aattttcaat
     4801 aattcctttg acattgacag gaatatcttt cgctaataca gacatcatcg tagctgctcc
     4861 catcgaacca ccataaagta aaatcttttg agatggccgc tctcgaactt cttgatttat
     4921 ccagtaaagg acgtcatctg actcatattg tccataggtc acataatcgc ctccgctatt
     4981 accagtcgct ctggaatcaa caaataataa attatacccg gccttataaa aatcattgta
     5041 ttcttctgta acgatcccgt tattccaatt ccctcgaaat ccgccatgga tcatgactgt
     5101 tttatctgaa ccttgatcga cataatatgc atatacatcc atcattttgc catcttgttt
     5161 gtttagtaca gtttgatgaa gttgttttgt atttggatag aagcctgtgt tttctgtagg
     5221 cgttgtccca tcatctgtac ctccaagcat cgacatcaac acactgtttt ttgcaaaagc
     5281 gatatcgtac aatagataag tagcaatcag atgtttgata atctctttat ctacaccata
     5341 atcattaaca gcaagctcag tgactaataa ctctattaat tttgacttga tgtcttctgt
     5401 tggtccatta agtatttctg tcatctgttt accgaagtaa ttcaaatatt ctttaccatt
     5461 gaaattttca cctttaccga tatcatcatt ttctgtccca ttctctttgt cacttacttt
     5521 agcagaagag gattcgtcgt ttgacatttt tgtagctgtt gcttcttctg tttgttggtt
     5581 atctgtggta gcggtcgttt cttgatctgt gctagtaaca gatgtctcaa ccgttgtttc
     5641 agtatttgtt tctgtttgtg aagtactcga tttcgtttca tttccagcat cagtagtttc
     5701 tgtggtttct acctcccgag attccgattc ggaagaaatg ctctctccat atactgtcca
     5761 ttgcgggacg cttgtactta cagcaaataa gatgaccaac gctcccttta atacttgttt
     5821 tgttctcaag ctattcactc ctttttatgt ttctaggaga attatatgta aacgcaaaca
     5881 ttttttatac aaaaaataaa tggtttatcc cataaaaata gaacaggatt attttctcat
     5941 tttacgtaat aaagcagaat ggataaacca gtatccattc tgcttttttt aaacgaagaa
     6001 acaacttatt gctccaaaaa tgtcagaaat cgtttatccc aaattttctc gtaaaaagcg
     6061 attgtttcct ttgctttcat aaaaggatta tcataagttg tcgttgctcc tctacacatc
     6121 ataagctggt atcctaatcc atgagcaaac ttcacagtcg tatctataca atactctgct
     6181 tgtgccccac ataattcgat cgaatgacag tccaattcag ttaataaaac actcaaatta
     6241 gtcttataaa atgaattcgc atgggttttt ctcacaaaat aactgtgctc aggaatagat
     6301 aattccgaca atagctccca tggttttgtt tgtggcacta attgagcatc ttcgtgttga
     6361 acgaaaataa ctggtttccc aatcgtttcg tatgttttga ttcttttatt cactaatgct
     6421 gtcactgttt ctaattcgtg tatcggcgaa gaatcaaaac ataccccttt ttgtaaatcg
     6481 atcactacta ttgcgtcggc tgtgtttgac atacttgtgc cttctttctt ttttcttgat
     6541 ttaatagtat ttcttatcta gctagttcca ttatacgtga caacattttc ttattttaca
     6601 cctctaaatg gcacttttcg actatttctt catgttatta tttcataaat acttagctat
     6661 tggaggaatg tatgatgatt aaattgtata caggttctgg ctgctcgtct tcaagacgcg
     6721 caaaacgttg gcttatcgtc catgatatac cgtttatcga acagaactta attcgtcaac
     6781 cactaaccaa aaatgaattt atccacttac taaaattgac aaacaacggc gtgaccgaaa
     6841 tgctctctac tcattctatt agttataaaa atcttgttca cagaatagat gaactcagtt
     6901 tgagtgaact atatattgag attcgtacat ccccttcttt attgaaaaaa ccgattatct
     6961 acgaccaatc tgcaaaactt ttgactggat ttagcgaaga taaaatcagg atgttccttt
     7021 ctaaaactaa aagaaagcaa gagctcaacg aactcttgct ttctggtcat cttgtcgatt
     7081 ttaaagaaaa taatcttttt gcaatgtctt aattttggca taaacaatct gcttttctta
     7141 attaacgaaa tccaattttt ccaatatgga tctatttttc tcattcctga tttttatgat
     7201 ttcagctttc agacgcgccc aatcattttt tgtaggttgt ccattccagt aaaatatctg
     7261 tgtattcgtt tgatcatatg cctttttgtc gataggataa tcgctaatga caatatcagg
     7321 taactgatca gcggatgcac tatattttat tgggcgttca gtcatagata atatcctttt
     7381 ctttgcccat aattcctgtg ttttaccata ctttgattga atatagaata tgacaggttg
     7441 ttcgtgtttt ataaaaatat ccctgactaa catacagtat tgaaaaatca accgtctgtt
     7501 cttttcataa taagtattaa gctctggaat tctgcgagct accccactaa aaaactgttg
     7561 catgattgga tacatcactg gaaatatatg ctgaaaatct ctgtcttctg ctgattttcc
     7621 aaaagcatcc accttctttt ttgtcccaaa aacccttgaa tacaaatgga tcattgccaa
     7681 attgacatat agataatttt tttctgaagt tgtaaaacaa atcttgaagt ttttttcaaa
     7741 attttctaca atagacgatt caatccagcc ccaactttct tgtactttat ctggagtatc
     7801 caacaaatct tcttccaaat aggtgttcat tacaccaaac ataaaataaa ggtaagcttg
     7861 ctctgttgga gtgatgtgat tcatcgacca gaagtttgct tcaaaaaaag gctctagtat
     7921 gtaatgcgta aactcttctt ttgttatcaa tggatgcaca aatttgttca tctcttctgg
     7981 gacatacgta atcgagtggc cttgtttcat tcgttgcaac gaaattctta gtaggctgat
     8041 ccatttcttt ctatctatac tacgcgtata tggcgcatgt ttcgcaaatt tttctaatag
     8101 ttgacgttta ttttttatac ttgataatat atcagaatga ttttcgtcga atgactgcca
     8161 atacatcaag tgaaaaaagt atcggatacg gtattcttct ccttctatcg gattaccttt
     8221 tttcaagctg caagataaat gatagttttt caaaatagta ttaagttctc ttaattttct
     8281 ttgaatcgta cgcaaactta tataattttt tgactaagat tttctaagga ttgataagta
     8341 cctttgtaaa gtgaatccag taaaatataa ccaatcgttc cctctaaaag tatagaataa
     8401 actttactca gtaaaaactg agaagaaaac tcgactttta tcatcttgtt ttctgaaaga
     8461 aaagataatt tatattctgg atacctttcg caaaacgatt ctgcttgttg aatcaccgaa
     8521 ttcatcttag ccagggtgta gtttagatct aaagcgatat ccttcaccac atataatttc
     8581 tcgtctcttt cataacaata gttcaaaaat tgatatactt gcctttcatt gatatcaata
     8641 aattcttcaa acaaagtaca tactcctttt tatctattat ttagacttat tccgacagct
     8701 acatgaataa cgtacaaata gattcgttac tgatttttca gagatactac catgcgcagt
     8761 gtgcattgtc aattattctt gctcttattt tccatttgtg ttcctgttat ttcctgtttc
     8821 tcaaatatgt tagtaaacaa aaatagatct ggcaaagcta caacttcgcc aggcaataca
     8881 gcactctcga atgaaatatt cactcaaaaa tctatcgttt tattcaattt tagatctgtt
     8941 gcttttattc tttatttcac tcgatggaag agagaacgaa aatagcttgc tcctacaatc
     9001 aaaatgagta acacacccat ccatgtaatt agcttgttca aggttctact accagtttct
     9061 ggtaaatcgc tttttctatt atctgtttct ttcgcttttt cttgatgatc aattccacga
     9121 gaaacaaaat caggctgttt tagcacactt tttaggttgc tccctgcatt tctcttttgt
     9181 tccttagttg gtatgatagg taccaaatca agttcattat ttacttttcc tgtgctgctt
     9241 tcgctgctcg tgcttcctgt gctactctca ctactcatgc ttcctgtgct actttcacta
     9301 ctcgtactct ccgtactgct ttcgctgctc gtgcttcctg tgctactttc actactcgta
     9361 ctctccgtac tgctttcgct gctcgtgttt tctgtgctaa tttcactgct cgtactctct
     9421 gtactgcttt caccgctcgt gttttctgta ctgctttcac tacttgtgct atccgtactg
     9481 cttggcggtc caacaactgt ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt
     9541 ccatcataaa aaacaatcaa atcaacttta tctttactct tgatctcatc aatcgatagt
     9601 tctgcagaaa tcattattgt atactcaata ccagcggtcc attgtacgtc ttttaaataa
     9661 acttccccaa taactttttc tgaacttcca ttaccatccc aaccatatgt tagaacagca
     9721 tgtacatcac tagcattcac gcttttgttt ggtttgactg gaatagtaat gacttgttgt
     9781 gtcatgtcat aaacaatccg atcagttttc acagtaaatg aaagattctc atttgcccgt
     9841 tcatttgcag aagtactgat tccaatcatt gctgcaatta tactcagccc aagaataaca
     9901 aataatacgg tcgctgtttt cttcatttct atcactctcc atataattac cttctatttg
     9961 ataaaggaat tatataaaat gaaaatgatg agaacacgac agtcagttgt gaaaatctaa
    10021 caaaagatgt cgtgtattcg cttgaatttg tttacttttt cgtttaatca atgacagact
    10081 taaaaaagca agaccgatat cagccttgct ttcttcatct tttattgata aacagcagtg
    10141 tatttcttca tccgctttgt tttatatgca tagagtaaca aaccaacaat caagactaac
    10201 aagaataatg tagttgatat agatgcttca gccccaaagt ttcccccagt aattattgta
    10261 ttttgcgatt ggatcagagt ggagaaaaaa ccttgttctt gcgttccgct gactggtata
    10321 cccaaaatag ctccttgtgt ccaattccaa gtaatatgag caccaatagc cgtaaataaa
    10381 cttcctgtac tgtagataag tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact
    10441 gccagaaatg tcatgttagg attcaatccg tgcaaaagag caaataatgc agcagatcca
    10501 atcattgcta ttaacggacc aaatctaagc atctgatgct gaaaaaagcc acggaataaa
    10561 agctcttctg atgcccccac tgcaataaat aaaataaaac tcgttatcag cggcacaatg
    10621 tttgcgtttc ctgaatctaa ccgatacgcc cccgtcaaat ataatggact agtaataagc
    10681 agaaacactc caattccaac aacgagacct cccgctaaat acccccagtc ttttcctcga
    10741 acagaaacac cataatcttc tattcttttt ccttcgacct tactagcaag aaataaagct
    10801 agtaaaaaaa cagctaaatg tccgaccgta ccggcaattg ctccaatcca ttcactattt
    10861 ccaataaaca tatccgcaag tgcattattg acaagaattc ctaaaacaaa accaacaaag
    10921 actattaaaa cagatccaat caaccgaata atcctgatca tctttcatcc ctcctttata
    10981 ttaagtttaa caaccctttt ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca
    11041 tagagagaat agatttttac gaactaagaa tatctacagc aaccttacga aaatgaaaga
    11101 ctgtctccct aattcttctg ttagactgtt tttaataagg aaaggatggg aaattttttg
    11161 aaaaaacaac tactaattat gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt
    11221 agcaagtcca atgaaacgat tgcaacgttt aaaggtggaa cacttacagt tcaggatttt
    11281 tatgataacc caaaagttaa atcaaataat caaacactat taaaaaaaat gattgtcttc
    11341 aaagcatttg atgatgtata cggaaaagag atttcttcaa aggaagtaac aaaagaatat
    11401 aatgaacaaa tcaaaaaact cggtccaaac tacaaggaac aattaaaagc agttggccaa
    11461 acagaagaga catataagaa actctttaaa cagatgctgg ctttccaata cggtttgaaa
    11521 gccaatgtaa aattaacaga taaagatcta gacactgctt ggaaagaatt ctatccagaa
    11581 gttagtacac agatcattct attttcgact aaagaagaag cagataaagc aaaaaaagaa
    11641 gcaaatgaag gagaaaattt ctccaagctt gttcaagctt atggaaaaaa taaaacatta
    11701 aaggaaactg atggaaaaat gaattttgat tcaaccaatc cagagattcc tactgaagta
    11761 aaaaaagcag catttaaact aaaaaacggg gaagtttctg atatcattcc tgtaacagat
    11821 ccaacgactt atcagcaaag ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt
    11881 tctaataaag acaaatacaa atctgagcta gaaaaaattg ctaccgaagc aaaaactctt
    11941 gatacggaat tcatggataa aacaatccgt aaagtgatga aaaaagacaa tgtaactatt
    12001 aaagatcctt atgtaaaaaa tatattcaaa taaacaaatt tgacccaatt ggggatgtgg
    12061 caaaatttga gaagtgattt ttggaaattc cttcttattt ttgaggcaaa ccacttttgc
    12121 cacaacttca tcggggcctt tttttatttg taccaaaaca aatcactgga tttgttacaa
    12181 tcatgaggat ataatttaaa aaagtttttt atttctatca catgaatcaa tgcgccatta
    12241 agtagattct ttcatgcttc cctatttttt cacttgccgt atcgcttcaa aaacaactat
    12301 cataaactca aactcgactg aaaagttatt ttaaaaaaga ctaaaaatat cccttctgga
    12361 cttgttcttc ttattctgtt atactagaga aacacgaaag atatttttat gcgacacttt
    12421 tcatttgttt ttatgaaatt tattcaaaac tggcgtaact tcagagttac aaggatgtat
    12481 tagaggttag ggaatacatc gtttaaatga gaaataagta atagttagta atagtcaccc
    12541 aaactttaaa ttaatgaata ttgaatcgtc agatagcctc ctcgagtttt cgagaaggct
    12601 atcgtttatt ttatggagaa attgattcag aagtaatcag cgaccttttt tagttcacca
    12661 atcgtttctg cttggaaagt ttgcacccaa tcttcttgga agcccctcac tgctttatca
    12721 atcgagggca tctctaatac ttgatctagt agctctttac ctaacgttat cgctgagctc
    12781 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
    12841 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
    12901 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
    12961 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
    13021 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
    13081 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
    13141 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
    13201 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
    13261 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
    13321 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
    13381 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
    13441 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
    13501 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
    13561 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
    13621 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
    13681 acaaccgttt caaagatcgt catctct
//
LOCUS       DAYLZO010000076        13287 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.077, whole genome shotgun sequence.
ACCESSION   DAYLZO010000076 DAYLZO010000000
VERSION     DAYLZO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.077"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1057
                     /locus_tag="ACGVJG_002030"
     CDS             <1..1057
                     /locus_tag="ACGVJG_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFJ6557956.1"
                     /translation="INVIGKDEQLKNNKENDGKPEYTDKPITHTYLKELNQATFGTDS
                     TIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYENGNKWSTPPFDFAELKR
                     LFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQGAEMLATMIHLNRGTPY
                     IYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKEAFRRIKAKSRDNSRTPM
                     QWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSYYKKLIRLRKTYPIVAQG
                     DYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPEGFTEANILIDNYSDTVL
                     HQSLELKPYQAIALLKN"
     gene            complement(1117..2298)
                     /locus_tag="ACGVJG_002031"
     CDS             complement(1117..2298)
                     /locus_tag="ACGVJG_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFJ6557957.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            2550..4073
                     /locus_tag="ACGVJG_002032"
     CDS             2550..4073
                     /locus_tag="ACGVJG_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFJ6557958.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(4142..4870)
                     /locus_tag="ACGVJG_002033"
     CDS             complement(4142..4870)
                     /locus_tag="ACGVJG_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFJ6557959.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(4883..6163)
                     /locus_tag="ACGVJG_002034"
     CDS             complement(4883..6163)
                     /locus_tag="ACGVJG_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFJ6557960.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(6304..6906)
                     /locus_tag="ACGVJG_002035"
     CDS             complement(6304..6906)
                     /locus_tag="ACGVJG_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFJ6557961.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7151..7357
                     /locus_tag="ACGVJG_002036"
     CDS             7151..7357
                     /locus_tag="ACGVJG_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557962.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            7669..8388
                     /locus_tag="ACGVJG_002037"
     CDS             7669..8388
                     /locus_tag="ACGVJG_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFJ6557963.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(8469..9152)
                     /locus_tag="ACGVJG_002038"
     CDS             complement(8469..9152)
                     /locus_tag="ACGVJG_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFJ6557964.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            9370..11556
                     /locus_tag="ACGVJG_002039"
     CDS             9370..11556
                     /locus_tag="ACGVJG_002039"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFJ6557965.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            11902..12312
                     /locus_tag="ACGVJG_002040"
     CDS             11902..12312
                     /locus_tag="ACGVJG_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFJ6557966.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            12326..12682
                     /locus_tag="ACGVJG_002041"
     CDS             12326..12682
                     /locus_tag="ACGVJG_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFJ6557967.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         4822 a         2230 c         2525 g         3710 t
ORIGIN      
        1 tatcaatgtt atcggtaaag acgaacaatt gaaaaataat aaagaaaatg atggcaaacc
       61 tgaatatacg gacaaaccaa tcacccatac gtatctaaaa gaattgaacc aagcaacgtt
      121 tgggacggat tccactatca tcacagtcgg tgaaatgagc tctacgacga tcgaaaattg
      181 tatcttgtat acagaaccta gtagagaaga attatcaatg gtctttaatt tccatcattt
      241 gaaagtcgat tatgaaaatg ggaataaatg gtctactcct ccatttgatt ttgcagaatt
      301 aaagcgacta tttcatacat ggggagaaaa gatgagtgag ggtaacggct ggaatgctct
      361 attttttaat aatcatgatc agccacgtgc gctcaatcga tttgtagcta tcgatcagta
      421 tcgaaaacaa ggagcagaga tgctagcaac catgatccat ttgaaccgtg ggacgcccta
      481 tatttatatg ggagaagaaa tcggcatgat cgacccagac tattcatcga tttccgacta
      541 tattgacgta gaaagtttga atgcttataa gttactcctt gaacaaggat tcacagaaaa
      601 agaagcgttt cgtcggatca aagcaaaatc tcgcgataac tctcgaacac ccatgcaatg
      661 gtcagacaca gaacaagcag gatttactac agggaaacct tggttaaaag ttggaggcaa
      721 gctagaagag attaatgtag aaaaagaact atcttctgaa gattctattt tgtcctatta
      781 caagaaattg attcggctga gaaaaacata tccaatcgtt gcacaaggag attatcatgc
      841 ttatgaagca aatcatccgg aagtttatgg ctttcttcgc caatttgaag gacagcagct
      901 gcttgtatta acaaatttct atgataaaga tacaaggatc gccatcccag aaggattcac
      961 agaagccaat attcttatcg ataattattc cgatactgtt cttcatcaaa gtctggagct
     1021 aaaaccatac caggcaattg ctttgctgaa aaattaaaac aaaaacacaa aacgtcgttc
     1081 ctacgagcta ttctcgtaag atcgacgttt tgtgttttat ttaaaataat cttcttcaga
     1141 aaatgaagag ttttcatact ctcctttatc gccataaaat ttattgtagc gttgtttgaa
     1201 tttacggaaa ataaaagtaa cgagtaattt gactaacgta tagataggaa taccaaaaat
     1261 aacgcccaca attcccatta gatcacccat gacaagtaaa acgattaaga tcgtaatcgg
     1321 atggatctgt aaacgatcac ccattactcg aggaatcacc aagtcaccgt ggagaagttg
     1381 tacggcaaac cagacaatga caaatttgac agccataaaa gtagagtctt ggaatgcaat
     1441 gatcaaacct ggaaagaagg cgataaacgg cccaatatat ggaatgatac ataacaaccc
     1501 agcagaaata gctaaaacac tagcaaattc taatccgatc agtaagaaac aaacccagta
     1561 gactgctcca aggataaatg aagcaatgat ttgccccttt agaaatgaac ccaattgttg
     1621 attagcaatc tttgtcaaat ttttaaaatc tgtacgaaat gctggtggga caatcgctaa
     1681 aactgactga taaaattttt gaggatctct caaaaggaaa aaagcgacaa ttggaccagt
     1741 aaataaagta ataaaaatag ctgtaacagt tgaaaaaatg ttgctgatcc gttgtgcgcc
     1801 actttcccaa taatttccaa taaatccagc tatatcagac gtcacgtcgt caatagaagc
     1861 gaaaaattga ttaaatgaat cggcaaaagg tgttttctcc aaaaattgtc ggagactctc
     1921 ttgaaaatct tgaaacaaat ctggcaaact actgatcaat gtctttgcct gttcttcaag
     1981 aaaaggaata agccatatgc ttccaagccc caataaaaat aaaataatca cataaacgat
     2041 aatgactgac aagttcctcg aaaggtgctt gctcaaacgt ttgatgactg gttccaaaag
     2101 ataatacaag atcagtgcaa acaataatgg gggcaagata gtagcgataa tgattccgac
     2161 aggcttgaat ataaacgaga tctgatggaa aaaataaatg atcagcgcaa gtaagataac
     2221 tattcctaaa acatagtaag aggtttttcc tcctaaaaag cggatcaaac gattcgttgt
     2281 atctgtttct tttttcactc tttttcctcc tttatctata aattcattat agacaaaagc
     2341 gggtataaaa ggaaaatgat agaggttgat tgcaaaaata tcgtcaatca ttcatataaa
     2401 ctttctcatc aattaaaata agaaaacgct tgacagttct tttaaccata tgataaatta
     2461 aatcctgttg ctaaaacgta actattacgg tttgcgaaaa gcgaatcgga acattcaaat
     2521 gaaagaagat gagaagagga gtgtcataaa tgaaacgatt gataggtata ttggccatgc
     2581 tggttttagc aggtatgtta actgcttgcg gggcaagtgg gaaagccgag gacagtaaag
     2641 aaaaattgag cgtgatgacg accttttatc ccatgtatga ttttacaaaa gcaatcgtcg
     2701 gggatgaagg agaagtagag ttattgattc cagcagggac tgattcccac gattatgaac
     2761 cttctgctaa agacatggca aagatccaag atacagatat ctttgtttat aatgatgaaa
     2821 acatggaaac atgggtcccc gcaatccaaa aaacattaca agaaggcaat gtccatacga
     2881 taaaagcgac agaaggtatg cttttacttc cgggaagtga agaaggacat gatcatgacc
     2941 atgaacacgg ggaagaaggc catacacatg agctagaccc acatgtatgg ctagctccaa
     3001 gtttagcaat caagcaggta gcaaacatac gtgatcagct gattgaagct tacccagaaa
     3061 aacaggaagt atggacaaaa aatgcagcag cctataccga gaaacttcaa gcactccatc
     3121 agctttatca agaaaccttt aagcaagcaa aacaaagatc ttttgtcacg caacatgctg
     3181 cattcaacta tctagcgtta gaatatggat tgaatcaagt atcgattgct ggtctttctt
     3241 ctagtgaaga accgtctgcg gcgagaattg ctgaactgaa acactttgtt aaagaacatg
     3301 gaatcaacta tatttatttt gaagaaaacg ccaaagatag tattgccaga acgttagcaa
     3361 acgaagcagg tgtatcttta gaagtattga atcctttgga aggattgacg aatgaacaaa
     3421 tagaaaatgg agaaaactat ctttctatca tggaagcaaa cctcgaagcg ttgaaaaaaa
     3481 cgacagagac agaaaacccg ctagaagata ctttgacccc caaaaaagaa aagagcgtct
     3541 acaacggtta ttttgaagac gaggatgtac aagatcgctc tctttcagat tgggcaggaa
     3601 cttggcaaac agtagatacg tatgtagaag atgggacttt tgatcctgtt tttgaatata
     3661 aagaaaaatt gaatcaggat aagacagcac aagaatataa agaatattat acgaagggct
     3721 atcaaacaga tattgcttca atcaagatca gtgagaaaga gatggcattt acgtttaaga
     3781 atggaacaac gaaacgttca aattatcgtt atgtagggaa agaaatcttg acctacgagg
     3841 caggaaaccg tggcgtaagg tatctattcg aagcagaaga taaggaaagt ggcgctttta
     3901 gatatattca attcagtgat cacacgattg caccggctga ctccgaacat tttcatttat
     3961 atgcaggaaa tgaaagtcaa gaagtattgc tgaaagaaat ggagaattgg ccaacatttt
     4021 atccagatac aatggatgga cgtgaaattg cacaggaaat gcttttgcac tgataggcaa
     4081 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
     4141 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
     4201 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
     4261 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
     4321 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
     4381 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
     4441 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
     4501 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
     4561 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
     4621 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
     4681 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
     4741 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
     4801 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
     4861 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
     4921 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
     4981 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
     5041 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
     5101 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
     5161 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
     5221 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
     5281 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
     5341 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
     5401 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
     5461 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
     5521 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
     5581 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
     5641 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
     5701 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
     5761 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
     5821 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgc
     5881 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
     5941 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
     6001 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
     6061 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
     6121 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
     6181 gatttttacc aaattatagc acaattggcg tattattttt ttaatttttc agggattttg
     6241 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
     6301 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
     6361 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
     6421 agctgagaaa aataacgtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
     6481 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
     6541 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
     6601 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
     6661 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
     6721 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
     6781 tagttttcca agattattca ataaaacttc taagacaaat tttacttgga catacaccat
     6841 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
     6901 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
     6961 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
     7021 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
     7081 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
     7141 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
     7201 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
     7261 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
     7321 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
     7381 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
     7441 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
     7501 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
     7561 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
     7621 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
     7681 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
     7741 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
     7801 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
     7861 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
     7921 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
     7981 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
     8041 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
     8101 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
     8161 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
     8221 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
     8281 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
     8341 tacactacaa ataacccgaa agcaaaggga atctttacaa ctaaataata taaaaactaa
     8401 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
     8461 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
     8521 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
     8581 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
     8641 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
     8701 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
     8761 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
     8821 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
     8881 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
     8941 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
     9001 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
     9061 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
     9121 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
     9181 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
     9241 tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca
     9301 acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa
     9361 atcaatcagt tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag
     9421 aacaagtata aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat
     9481 gaagcggctt atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt
     9541 gaaggaaaca gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa
     9601 gaacaaaaag cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct
     9661 gggagcggca aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa
     9721 gtagacgaag aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa
     9781 aaacgagtag ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt
     9841 atctttttta ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga
     9901 atacaaggag acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg
     9961 atacgtaaat ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg
    10021 ttagactatt ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa
    10081 aaatatgatt ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg
    10141 ctcacggaac tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc
    10201 caaaatctga aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat
    10261 gattatctat tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa
    10321 cggatgacaa aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc
    10381 tatggattca gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc
    10441 aaaactcttt tcttattaac taattatcgt tctcgggcag ctattgtcca aggagcaaat
    10501 agattgatca gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa
    10561 gaagcaggtg ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta
    10621 atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta
    10681 taccgatcag cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct
    10741 tttgtactgg aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat
    10801 ttctatcaaa aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc
    10861 taccagcaat tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat
    10921 caatactttt ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc
    10981 cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca
    11041 gaccagcatc aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct
    11101 aaggaaatcg accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact
    11161 ttcagaagac tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc
    11221 cgcttgattg cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa
    11281 agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct
    11341 tataacggag cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagta
    11401 acgacgatag aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt
    11461 ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc
    11521 aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt
    11581 ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat
    11641 ttcagcttat tttcgaagaa gctatttctg gaacacagtt tattcggaaa agggaggtgt
    11701 gacaagaatt ttgtcacacc tcccttattt attttgaaaa tgagaatact attatcaaaa
    11761 tagagaatat atagaaaaat tctcattatt caaacgtaaa taatcagtta ttttgatttt
    11821 ttttatattt tattagctaa taattggtat tttaaaagaa attactgtag aattggatta
    11881 taattaaaaa ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt
    11941 agaaaagcaa gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt
    12001 gccgaaccgt taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt
    12061 caagatatta tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa
    12121 ttaactttaa agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct
    12181 attttgattg atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt
    12241 gtccctcgtg aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc
    12301 ttaaattcat agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt
    12361 aacaagtctg aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga
    12421 cttagaagaa cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt
    12481 gcaagcagaa cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct
    12541 ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag
    12601 acgtcctttg attattatcg atgaagttct atttattgga tacaatgagc agttgatgga
    12661 acagcttatc ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata
    12721 cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat
    12781 ttttctacta tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata
    12841 tagagttaaa aatatattga catgtgtaaa tttacatgat atattcattt ctgttgtttt
    12901 aagtagcaaa gcaataaaat agtattttaa aaaacatata aaatagttgt tgacatgtta
    12961 gaaatgacat gataagataa ctgagttgtt aaaaaagaac cacttgtgaa gcttgattct
    13021 atcgaaaaaa acttcaaaaa agttcttgac aaataacaaa tgataagtta tgataaatga
    13081 gttgctgtaa gatttcgaaa aacgaaatca aaaaaacttt taaaaaagag ttgacaatta
    13141 cttgtctctc tgatatgata taaaagttgc tacggcagcc aaaaacgaat cggggaacac
    13201 tcctgatgaa gtagaccttt gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg
    13261 tcttgattca attcaagaca acaaaca
//
LOCUS       DAYLZO010000077        13174 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.078, whole genome shotgun sequence.
ACCESSION   DAYLZO010000077 DAYLZO010000000
VERSION     DAYLZO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.078"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..296)
                     /locus_tag="ACGVJG_002042"
     CDS             complement(<1..296)
                     /locus_tag="ACGVJG_002042"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFJ6557968.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVI"
     gene            complement(801..1496)
                     /locus_tag="ACGVJG_002043"
     CDS             complement(801..1496)
                     /locus_tag="ACGVJG_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFJ6557969.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(1586..2686)
                     /gene="ychF"
                     /locus_tag="ACGVJG_002044"
     CDS             complement(1586..2686)
                     /gene="ychF"
                     /locus_tag="ACGVJG_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFJ6557970.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2695..2886)
                     /locus_tag="ACGVJG_002045"
     CDS             complement(2695..2886)
                     /locus_tag="ACGVJG_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFJ6557971.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2907..3797)
                     /locus_tag="ACGVJG_002046"
     CDS             complement(2907..3797)
                     /locus_tag="ACGVJG_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFJ6557972.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3784..4551)
                     /locus_tag="ACGVJG_002047"
     CDS             complement(3784..4551)
                     /locus_tag="ACGVJG_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFJ6557973.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(4563..5276)
                     /gene="rsmG"
                     /locus_tag="ACGVJG_002048"
     CDS             complement(4563..5276)
                     /gene="rsmG"
                     /locus_tag="ACGVJG_002048"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFJ6557974.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(5508..7409)
                     /gene="mnmG"
                     /locus_tag="ACGVJG_002049"
     CDS             complement(5508..7409)
                     /gene="mnmG"
                     /locus_tag="ACGVJG_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFJ6557975.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(7426..8823)
                     /gene="mnmE"
                     /locus_tag="ACGVJG_002050"
     CDS             complement(7426..8823)
                     /gene="mnmE"
                     /locus_tag="ACGVJG_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFJ6557976.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            9122..9505
                     /locus_tag="ACGVJG_002051"
     CDS             9122..9505
                     /locus_tag="ACGVJG_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6557977.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9673..10431)
                     /gene="jag"
                     /locus_tag="ACGVJG_002052"
     CDS             complement(9673..10431)
                     /gene="jag"
                     /locus_tag="ACGVJG_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFJ6557978.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(10449..11267)
                     /locus_tag="ACGVJG_002053"
     CDS             complement(10449..11267)
                     /locus_tag="ACGVJG_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFJ6557979.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(11264..11620)
                     /gene="rnpA"
                     /locus_tag="ACGVJG_002054"
     CDS             complement(11264..11620)
                     /gene="rnpA"
                     /locus_tag="ACGVJG_002054"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFJ6557980.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11738..11872)
                     /gene="rpmH"
                     /locus_tag="ACGVJG_002055"
     CDS             complement(11738..11872)
                     /gene="rpmH"
                     /locus_tag="ACGVJG_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFJ6557981.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            12396..>13174
                     /locus_tag="ACGVJG_002056"
     CDS             12396..>13174
                     /locus_tag="ACGVJG_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFJ6557982.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVS"
BASE COUNT         3560 a         2814 c         2342 g         4458 t
ORIGIN      
        1 atgattacgc cactttcaga acgtttgact tttcttactt catccgcttg ctggctgatc
       61 gtcatgtttt tatggatcac acctagtccg ccttgtcttg ccatagcaat ggccatcttg
      121 ctgtcggtaa cagtatccat acttgcactg atgattggaa tattcaactt aatattttta
      181 gctaattgta cgctcatatc cacttcgttt ggtaacacgt gactttctgc tggtatcaat
      241 aagacatcat caaaagtaaa tccttttttc gtgaactttg tttcccagtt ggacattttt
      301 agtaccactc ctctaattta gtttatctat taaaataatt gattttttca tttaagtcaa
      361 tgatctactt ggaatcaagt tagctttctc atagaatacc aatataaagc gtttacatct
      421 gtgtgtatta aaagagattg taaaaaagct gtcctgcatc aagtaataag aacagccaat
      481 caaaaacagc ttctcatgtt tttgaattgg ttgggtttat taccgcagat gcaaactttt
      541 gaacaccgtt tagtggagag gctgtgaaaa agctgcttag tttcgagtag taagaaaaat
      601 tttgtgaaaa tggctttcca catttttgca taaattggac ttaataccga gaaactggct
      661 tttgaacacc gtttatcgta aaaagggact gtgacaagac ttttgtcaca gtcccttact
      721 attatttaac tatggataaa caaaatgagg ttctttacta cccctagata tctagcaaaa
      781 tagctgatct ttttgctagt ttatcttctt gggacggata aacttccaat aatcccgtat
      841 tttttcttca agtagtaacg gattgctaaa gcagttgccc cgataatgat aactactact
      901 ggatctaata ctggattgat aactggtggc aatagtgttg tcgcagaaaa cagtacgacc
      961 caaagaagtg tcgttccacc taggataagc aatgtcttac ccattcctgg tttcttgctt
     1021 cgatctgctc cgggatattc atattgatag atgtatttgt acatcatata aaagacccag
     1081 ccaccgattg cagaacctag aacgagtgta gaaaagccat aagctggcgt tgttccacgt
     1141 gaaaacaatc cggtaatcgc aatcatgata cttaatagac caagcaacag caacgtatta
     1201 tctaaccaca tcaataaagg ggtggatgaa ttttttactt ctgctggctt attgatgatt
     1261 gcttccgttc tttcagagac tgtcccaaac aattgacggg ctgttcttcc gcctttttgt
     1321 tctgcaacta aagtaggcag aatgtcatga agggctttta ctttttcttc ttcagacaaa
     1381 ttcgctgctt ctaaagattt tttcaaatca aaaatgtact gttgatttct tttggttaat
     1441 tttgtttcta aactattgtt ttcttttacg attgctcgta attgttctgg ttccattatc
     1501 taatagtcct ccttagccgt tccttttctc aacggtcgtc acaaaagaca ttctaccata
     1561 ttctacagct agtttgatag cccttttaca cattaaagcg gaataacatt acgtcgccgt
     1621 cttgtacgac ataatctttt ccttctaatc gaacacgtcc agcttctttt gctgcatgca
     1681 tgtttccgta atgatccaag tcttcaaaag agacagtttc tgcacggata aatccgcgtt
     1741 cgaaatctga atggatgatt cctgctgctt gtggtgcttt gattcctttt ttgaatgtcc
     1801 aagcacgtac ttcttgttcc ccagcagtaa agtaagtagc taagcctaac aaatcataag
     1861 cagcacggat caactgatcc aaacctgatt cttcgattcc taaagcttct aaaaattccg
     1921 ctttgtcttc ttcatctaat tctgcgattt cttcttctgc acgagcactg accacgatca
     1981 cttctgcatt ttcttctgca gcaaagttgc gcacttcttg tacataatga ttcgcgtctg
     2041 catctgctac gtcatcttct gagacattgg ctacataaag aattggtttt gtcgtcaata
     2101 aaaatagtga tttaacgatt ttttgttctt cttcagtaaa ttcgatcgtt cgggcagatt
     2161 ttccttcttc cagtacaggt ttgattttat ccagtacagc tagttctgcc actgcatctt
     2221 tatctttggt tttagcaact tttgctacac gagtatagcg tttgttgata gattctaagt
     2281 ctgctaacac tagttccaag ttgatcgtat caatatcttc tagtggatca acacgtcctt
     2341 ctacgtgagt aatattatca tcatcaaaac agcgaacaac gtgacaaata gcgtctactt
     2401 ggcggatatg gcttaagaat tgatttccca aaccttctcc tttacttgcg ccttttacga
     2461 ttccagcaat atctgtaaat tcaaaagtcg tcggaacagt ttttttcgga tgtacaagtt
     2521 ctgttaagcg ttgcaaacgc cagtcaggta cttctaccat ccctacgttg gggtcgatcg
     2581 tcgcaaaggg atagtttgct gcttctgctc ctgcttttgt gatcgcatta aacaaagttg
     2641 attttcctac gttgggcaac ccaacaattc cagcagtcaa tgccataaat ttattcactc
     2701 tttctctgca tttttttcaa ttactttttt taatttcttt tcaaaatctc gacgggtcat
     2761 catgacgata tgtccgcaat tcatacatct gattttgata tctgcgccca ttcgaatgac
     2821 ttcccagcga ttcgtctggc aagcgtgagg ttttttcatt tcaacgatat cgtgcaagtc
     2881 gtacataaaa atagcctcct ttttgtttat tcttcatcaa attgaatatt caagatatca
     2941 agaattcgag tcaaatcttt ttgagaaaga tattcaatct cgatcttacc tttcccattt
     3001 ttttcttgga tagctacact tgtaccaaat ttatccatca aacgatcttc gctttctcgg
     3061 atataatatg gtttttcctt tgctagcctt gggatttttt tcttttcttt tccatcattt
     3121 tgatttaact cagaaacaag ctgttccagt tggcggacag ttaaattttc agttactgca
     3181 cgattggcta atttcaagat caatgtttta ttttttaaac ctaacagcgt acgggcttgt
     3241 cccatagata aacgctgatc ttgtaccatt tccttgacaa gatccggtaa agaaagcaag
     3301 cgcaaataat tggcgatata aggtctgctc tttcctaaac gactagctac ttctgcttgt
     3361 gttagtttca ggtttttcat cagcatctca tatgcttctg cttcttctaa cggattcaga
     3421 tcttctcgct gcaagttttc cagcactgct acttgcatca tcgcttcttc gtcaaagtct
     3481 ctaacgatag cagggatcgt ttctttttca gctagtttgg acgctcgaaa acgtcgctcc
     3541 cctgcaataa tctcatatcc ttttactgcg gaacggcgta cgatgatggg ttgaaagaca
     3601 cctgattgtt caatagaatt agctagttcc tgcaaagata cttcttcaaa tgttttacgg
     3661 ggctgatacg gatttggtcg cagctcattc aatggtagct ggacaacggt atcatctttt
     3721 acatccaccg tctctaagtt ttctaaatct tgaaacagtg catcgattcc tcggcctaat
     3781 cctttacttt tcttcacgac ttaccacttc ctttgccaat gcttggtaga cttcagctcc
     3841 tcgagaacgt ggatcgtagt cgatgatcgg ttttccatga ctaggtgctt ctgataaacg
     3901 aatatttctt ggaatgatcg tttcgtaaac tttttcgcgg aagtatttac gtacttcttc
     3961 tacgacttca tttcccagat ttgttcgtgc atcatacatt gtcagtaaga ctccctcgat
     4021 ctctaattct ggattaaagt gtttttggac taaacgaacc gtattcagta attggcttaa
     4081 tccttccaat gcgtaatact cacactgaac aggtatcaaa atcgaatcac ttgctgtaaa
     4141 tgaattgatt gtcagatggc caagagaagg cgggcaatcg atcaaaatat aatcatactg
     4201 actgcttact tctgctaaag atccttttaa tcttgattct cttgccatca ttgacgtcaa
     4261 ttcgatctct gctcctgcta attggagagt cgcaggcaca atactcaaat tttcgtgttc
     4321 agtgatcaaa gttgcctcat caattggcaa ttcattgact agaacatcat agatgtctct
     4381 tgtcacatca ggttttctga tcccgacacc actggtagca tttccttgtg catccatatc
     4441 cactagcaat acttttttgc caaggctagc aagacaagca cctagattca ccgtcgtcgt
     4501 tgttttgccg acgccacctt tttggttcgc aacagatatt atttgtgcca tctcttctcc
     4561 tcctatttga ttggctgctt attaggcagt ccgggctttc ttgggtattt ctttggggtt
     4621 ggtttctttt tttgaataac gatgatatgc cgttgatcag agacattcgg caaggtaacc
     4681 gcctcttctt tgaggaattt accacctaat aaagcaatcg cttgttttgc ttctgtcaat
     4741 tcctcttcac tttttgctgc tttcaatgca taaaaatagc cttcttgttt agccaagggc
     4801 aaacacaatt ccgctaacac attcaaacga gcaaccgcac gagcagtgac aaggtcaaat
     4861 gatgcacgaa agtgaggatt ttgcccaaat gtttctgccc ggtcatgata taaggaagca
     4921 tcgattccta ataatcgggt taactcagat aagaaggtta ttcgtttatt cagagaatca
     4981 acgatcgtta tctgcagttc tggaaaggca atttttaaag gtatgctagg aaaccctgcc
     5041 cccgaaccaa catcgcataa agataaaggt tgcgtaaagt ctacttcaaa tgctaatgta
     5101 agtgaatcgt aaaaatgctt cagatatacg tctttttcat ccgtaatagc tgtaaggttc
     5161 atcttttcgt tccattcgac taatgtccga taataaatag cgaattgatt catttgatga
     5221 tcggtaagct ctatcccttt ttctgccaag gattggcgaa attcttctgg tatcataaat
     5281 attcctttca ttgtgaaaca gcaattgttt cacagattct ctccctactt taacgcaaaa
     5341 tctctagaca tgcaatcctt ccatatggat tcttaaaaat ttgaaaagta agaaaagccg
     5401 atagactttt tctttctagt atgactcata aaatatacac agaagagaga actagctttg
     5461 agcaatttct gcaaaaacta gttctctctt ctatttatta ttacgtatta cgctggttca
     5521 ctgactttcg caatcttccc ttgttcgata tagaccatta ggatacttag gtctgcaggg
     5581 tttacgccac tgattcggct agcttgggca attgtttctg gttggatttt ctgcaatttt
     5641 tgttttgctt ctgttgctaa tccgttgatt gctgcgtagt cgatgttttc tggaatcttt
     5701 ttcgcttcca tgcgttttaa tttttctact ttttccatgg cttttttgat gtagccttca
     5761 tatttgattt ggatttctac ttgttcgatt tcttttcgtg tcaattcttc attttctgga
     5821 ataaaccgta gaagttctgc ataagtaatc tcaggacgtc ttaaaaagtc gcttgctagt
     5881 acaccatctt ttaatggtgc cgcattaatc gttgctaaaa agtcttggac ttcttctgtt
     5941 ggtttgattc gtacattcat caaacgtttg atttcttgtt caactgcttc ttttttcttc
     6001 aaataattct tgtattgatc ttcttttact aagcctacac ggtgacccat ttctgttaat
     6061 cgcaaatctg cattatcatg acgtaaaagc agacggtatt cagcacgtga agtcagcaaa
     6121 cgataaggtt cgttcgttcc ttttgttacc aaatcatcga tcatgacacc gatgtaaccg
     6181 tcacttcgtt tgatcactaa aggttctttt ccctgaactt tcaaagctgc attgataccg
     6241 gcaatcagcc cttgtccagc cgcttcttca taaccgcttg taccatttgt ttgtccagct
     6301 gtataaagat tttcaacgac tttagtttct aatgtcggac ggagctggtg tggctctact
     6361 acgtcatatt cgatagcata tcctgtacgc atcatttcta ctttttctaa tccagcaata
     6421 gaatgaagca tgttgacttg aacatcctct ggtaaagaag tagataaacc ttgaacatag
     6481 acttcttccg tatttaagcc ttctggttcc aaaaataatt gatgacgtgg tttgtctgca
     6541 aaacgcacga tcttatcttc gatggatgga caatagcgag cgcctacccc ttcaacgatc
     6601 cctgtaaaca ttggggcacg atgtaagttg tctcgaataa tttggtgagt cgtctcacct
     6661 gtataggtca gccagcaaga ttcctgatct agtttatagg cactatctgg tgtgctgaag
     6721 ctaaaatgat tcggctcttt gtcaccaggc tgctcttctg taacagaata gtcgatcgta
     6781 cttgatttta ctcgaggcgg tgttcctgtt ttgaaccgtt cgatagcaaa gccaagttct
     6841 tttagatgat tcgctagtcc aatagaaggt tgagagttat tcggacctga tgaatatttc
     6901 aattcaccga taatgatttc tccgcgaagt gctgttccgg ctgtaataac aactgctttg
     6961 gcccggtagc tcgctcctgt ggacgtcact actccatgac aaacaccatc ttcgacgatc
     7021 agttcttcga cgattccttg tcttagcgtc aaatttggtt ctttttcgat cgtatgtttc
     7081 atttctgttg aataagcatg tttgtctgct tgtgctcgca atgctcggac agctgggcct
     7141 tttcctgtgt tcaacatacg catttggata taggttttat cgatattctt acccatttct
     7201 ccgcctaacg catcgatttc tcgaacgaca acgcctttag ctggtccgcc tacagaagga
     7261 ttacatggca tgaatgctac catgtccaaa tttattgtca gtaataatgt ttttgatccc
     7321 atacgtgcag cagcaagtgc agcttctgaa ccggcgtgtc ctgcgccaac aacgatcacg
     7381 tcatacgcat ctgcttcata atgttccaat cgtttgttcc tccttttatt ttcctaaaca
     7441 aaattgactg aataattgag tgattagctc atcttgtaca ctgtcgccga caacttctcc
     7501 tagatagtcc cagcaacgcg tcatatctat ttgaacgaga tccacaggca ttcctgaatc
     7561 aatacctgca atgacttctt caagtgagag ggaagctttt tctaacaacg cgatatggcg
     7621 ggtattagaa acataagtag cgtctctttc tcctgtttct ccgctgaaga ataaatcaga
     7681 aatagctgat tccaaacgat ctagtccgtc gtttttagct acggaaattg aaaaaactgg
     7741 ttcattttcg atcaatttct tcaatttttc ttgttccagc tgtgctggca aatctgtttt
     7801 atttagaaga atgatccgtt tcaatccact tgtcgcttcc aataattgtt catcttctgc
     7861 tgtcagtggt tcgctttggt ttagaacaag taagatcaag tctgcttctg ctaatgcttt
     7921 tcggcttctt tcgaccccga tccgttccac cacatcttct gtttcccgaa ttccagctgt
     7981 atcgattagc ttgagcggta ccccccgaac attgacatac tcttcgatta catctcgagt
     8041 cgttcctgca atatccgtaa cgattgcttt ttcttctctt aacaaatggt ttaacaaact
     8101 tgatttgccg acatttggcc ggccgatgat tgccgtactt aacccttccc gcagaacctt
     8161 cccttgcttg gatgtagcaa gtagcgcttg gattctttgt tggatcatct gcgctttttc
     8221 aagcaataac ttcgtagtca attcttcgac atcatcatat tctggataat caatatttac
     8281 ttctacttgt gctaatgtct ctaaaatctc ttgtctcaat gagcggatca aagcagacag
     8341 attgccgtct aactgattaa gagctagtcc cattgctttg tctgtttttg cccgaatcaa
     8401 atccatcaca gcttctgcct gtgaaaggtc aacacgacca ttcaagaatg ctcgttttgt
     8461 gaattcgcct ggctcagcta atcttgcacc ttctcttaac aatagctgca aaatttgatt
     8521 gactacaacg atcccaccgt gacaattgat ttctactaca tcttcccgcg taaatgtctt
     8581 tggtgctctc atcacggaga ccatgacttc atcgacaagc tgattgctct tgggatcaac
     8641 gatatgtcca taatggatcg tatgacttgg tacttctgat aatcgtttgt ttccagattg
     8701 ataaacttta tctgctagtt gcacagcttg atcgccactt aaacgaacga tactgattgc
     8761 accttctcca ggtggggtag aaattgccgc aatcgtatca aattctaaag taatttcagc
     8821 cattttttca cttccttttt cattttgcgc acaaaaaaag tgcccactcc accctttatg
     8881 cacaataaac ggtgaacttc gcactttgac tgcttgttct tgattaaatt ctggtcatag
     8941 attatcacac ttgctataag gaatcaagtg tttaaaacaa ttagatgaag ttgagcctgt
     9001 tctgcttcca ttaccttata tttaattgtc cttggatgtc tctttgcgtt ctttggttta
     9061 ttcgaagaga agatactgta taaacaaggc tttatctttc ttaaaaaaaa cggtacacta
     9121 aatggtagcg ctatctaaac tatacacttt tgaaaggaga aaatatgtga gcccgttcag
     9181 atctgctgca ttggaagaag aaagtgaccc tcggatcaac gatgtcgttt tatctacagg
     9241 gaatatcaac cagaaatata tcgtccgcga tatcgttttt gttgtggaat gcttagaagc
     9301 tgatttgttt gacccatctt ttcccaaaga tgaccttttc ttaacaacga agaggaaatt
     9361 aaagacgaag gtacttgaat atggtgcgaa tgcagtaatc aactgtcatt ttgagtatca
     9421 gcaaaaaaaa cgcgacggaa agtattttct ggaacttttt gcttatggaa cagtgatcca
     9481 atttatccaa acaacgattg gttaaatgaa acagacaaaa aaagcggatc aaaactgaag
     9541 acagtttcga tccgcttttt tctatcaaag ttagtgacta cttcatcgta tactctgata
     9601 agaatgattt ctagtaaaat atacttggtt tgaaaaactt tgtcaacgtg atttattcaa
     9661 aaagtaatgg aatcaataat attttttagc aggttctact actaaataac gataaggttc
     9721 ctcaccttcc gaatgtgttt ttacatgttc atctttgctt aatgcagcat ggatttgttt
     9781 acgttcaaat gcaggcattg gttctaagaa aacaggctgc cctgtccgtt ttactttttc
     9841 agccgtgcga tcagctaaac gctgaatgat ctcttggcgc ttttcacggt aattgcctac
     9901 attgactact acagacagct tatttcttgc taaacgatgg atgaagactt gtgccagata
     9961 ttgcaatgca ttcagtgttt ttccatgttt cccaatcagt atgccttgct tttgtgtttc
    10021 taaatggaaa acaaccatat tatttgattc tctttccatt ttgacaagag ctggagcatc
    10081 aagctgtttt gaaatttcag taagataaac tgccaattcc gttaaagctg cttcatcacc
    10141 tagctcactt acttcttctg ttttttcttc aagaatcgtt tcgttgacta cctcgatctc
    10201 agctgttggt tcttcttcca aaatttcttc tacagtctgt tctactgttt cagaaacttt
    10261 ttcgctgata cttggctgga tcgaaagccg tgcattcttt cgacccattc ctaggaaacc
    10321 tttttttcct tcttccagta tttcgatttc gacttggtct ttggccaaac ccaaatcagc
    10381 cagacctagt tggacagctt cgtctattgt tgcagcttca taaatcggca ttagcattac
    10441 ctcccctatt atttccgttt tttcttagga ttcattgctt tcttaagagc acgttctctt
    10501 tcgcggattc tccgtgcttc ttcttcacgc tcttttcgaa ttttgaaagg gttgttgatc
    10561 agcagcgttt gtacaacttg gaaggcattg gaaactaccc agtataatga aagaccgctg
    10621 gctaaattga cacccatcac taaaatcatt acaggcatca cgaaattcat gatcttcatt
    10681 gatgcattcg attcaatttg actcatgcta gataaatacg tacttgcaaa agtaaagatt
    10741 gctgctaaaa tcggcaaaat aaatgttgga tccgcgttcc ctaagttcaa ccataagaaa
    10801 tgcccttgtt tcaactctgg tacacgtgaa atagattgcc ataacgccat caagattggc
    10861 atttgtacta ataaaggaag acaaccagca tatggattga cgccattttc tgcatataaa
    10921 cgctgctgct cttctcttaa cttactttgt gtttccggat ctcttgaact atattgttgc
    10981 tgcagtgctt ttaatttcgg ttgtaattct tgcgttttac gcatgctctt tgtttggaaa
    11041 tgcatcaacg gcaataggat cacgcggatg atcagtgtaa ataaaatgat ccctatccct
    11101 gcattaccaa atgacaacgc cttgatcgcc tcagcaaagt aataaacaat ataacgatcc
    11161 caaattcctg tactctgtgc gctgacttcc ccagttccgc aaccggataa gacgatgacc
    11221 aaactaacga taccggccat caaaagtaaa cgtttgtatt tcttcactta ctcttttcct
    11281 ctctgtcgat tatttttgca agttttaaga cgtggactaa attggagcga acctcatcat
    11341 aagtcaaccc cttcacactt ggtctagcaa tcacaataaa atccaactcc ggatcaattt
    11401 cttcttttat agaataaact gctgctcgga tttttcgttt gacttcattg cggtttaccg
    11461 cgttgccgac cttttttcct acagataaac caactcgaaa atgaggctgt cccggttttt
    11521 ccaaacggta aacgacgaat ttgcggttag caaaagaatc tcccgtatgg aacacggctt
    11581 gaaactctcg ttcttttttt actcgatacg cttttctcat tagttttctt tccttttcta
    11641 tctctcactt acgctcataa tcataccata attacgattg tcctttcatc atttccctaa
    11701 aaaagagtat aaaaaaaacc actgaaaccc cagtggccta agctgaaaga acttttcttc
    11761 ctttgcgacg acggctagct aacacacgac gtccattttt agtactcata cgtttacgga
    11821 atccgtgaac tttttgacgt ttacgtttat ttggttggta tgttcttttc atatattcca
    11881 cctccaaaat gttcttatct attttataaa cacagacata ctttgatagt ataacgacaa
    11941 agtagtgatt ttgtcaatat aaaatagaag atcgattgtt aaaacttatc cacaagatta
    12001 tttttttcta tgggagtcct atgtggaaaa agttatgcac agggattatc cacagataat
    12061 aaatggctgg ttttacacag aaaacacacg tgtaaaaaaa gtaatccaca atcaggtgat
    12121 ttcctgtgta ttccgtctcc taactattgg tatatctata ttttttatcc acagatgcaa
    12181 gaggaaatct attgattttt acgagttttc cacctgtgca ttagtggtgt ggaaaacttt
    12241 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    12301 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    12361 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    12421 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    12481 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    12541 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    12601 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    12661 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12721 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12781 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12841 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12901 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12961 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    13021 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    13081 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    13141 acgagattcc gaaactgcaa gaacgcctcg tttc
//
LOCUS       DAYLZO010000078        13145 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.079, whole genome shotgun sequence.
ACCESSION   DAYLZO010000078 DAYLZO010000000
VERSION     DAYLZO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.079"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(77..149)
                     /locus_tag="ACGVJG_002057"
     tRNA            complement(77..149)
                     /locus_tag="ACGVJG_002057"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="ACGVJG_002058"
     CDS             530..1099
                     /locus_tag="ACGVJG_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFJ6557983.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="ACGVJG_002059"
     CDS             1086..1946
                     /locus_tag="ACGVJG_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFJ6557984.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /gene="yycF"
                     /locus_tag="ACGVJG_002060"
     CDS             2136..2840
                     /gene="yycF"
                     /locus_tag="ACGVJG_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFJ6557985.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="ACGVJG_002061"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="ACGVJG_002061"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFJ6557986.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /gene="yycH"
                     /locus_tag="ACGVJG_002062"
     CDS             4677..5993
                     /gene="yycH"
                     /locus_tag="ACGVJG_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFJ6557987.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="ACGVJG_002063"
     CDS             5994..6863
                     /locus_tag="ACGVJG_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFJ6557988.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="ACGVJG_002064"
     CDS             6918..7727
                     /locus_tag="ACGVJG_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFJ6557989.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="ACGVJG_002065"
     CDS             complement(7830..8459)
                     /locus_tag="ACGVJG_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFJ6557990.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="ACGVJG_002066"
     CDS             8629..9921
                     /locus_tag="ACGVJG_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFJ6557991.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="ACGVJG_002067"
     CDS             complement(9997..10659)
                     /locus_tag="ACGVJG_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFJ6557992.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="ACGVJG_002068"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="ACGVJG_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFJ6557993.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="ACGVJG_002069"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="ACGVJG_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFJ6557994.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2264 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccatt
//
LOCUS       DAYLZO010000079        12886 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.080, whole genome shotgun sequence.
ACCESSION   DAYLZO010000079 DAYLZO010000000
VERSION     DAYLZO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.080"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..920
                     /locus_tag="ACGVJG_002070"
     CDS             <1..920
                     /locus_tag="ACGVJG_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFJ6557995.1"
                     /translation="IGVPGRSNAFEISSRLGLDTEVIDEAKQLMNDESQDLNEMITDL
                     ENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREKEMEKAKKKANEVVSEAE
                     EKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEETLKKNKVLKKAKEQKTL
                     KPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDELTPVAPQKEPTQRVIHAV
                     RSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQVTIVHGKGTGALRKGITD
                     YLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            1007..1321
                     /gene="trxA"
                     /locus_tag="ACGVJG_002071"
     CDS             1007..1321
                     /gene="trxA"
                     /locus_tag="ACGVJG_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFJ6557996.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            1503..3281
                     /gene="uvrC"
                     /locus_tag="ACGVJG_002072"
     CDS             1503..3281
                     /gene="uvrC"
                     /locus_tag="ACGVJG_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFJ6557997.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            3426..4325
                     /locus_tag="ACGVJG_002073"
     CDS             3426..4325
                     /locus_tag="ACGVJG_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6557998.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            4318..5580
                     /locus_tag="ACGVJG_002074"
     CDS             4318..5580
                     /locus_tag="ACGVJG_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6557999.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            5741..7639
                     /locus_tag="ACGVJG_002075"
     CDS             5741..7639
                     /locus_tag="ACGVJG_002075"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFJ6558000.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(7969..8688)
                     /locus_tag="ACGVJG_002076"
     CDS             complement(7969..8688)
                     /locus_tag="ACGVJG_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFJ6558001.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    8878..8925
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            9025..9357
                     /locus_tag="ACGVJG_002077"
     CDS             9025..9357
                     /locus_tag="ACGVJG_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558002.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            9344..11347
                     /locus_tag="ACGVJG_002078"
     CDS             9344..11347
                     /locus_tag="ACGVJG_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFJ6558003.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            11366..11836
                     /locus_tag="ACGVJG_002079"
     CDS             11366..11836
                     /locus_tag="ACGVJG_002079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFJ6558004.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            11857..12441
                     /locus_tag="ACGVJG_002080"
     CDS             11857..12441
                     /locus_tag="ACGVJG_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFJ6558005.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            12456..>12886
                     /locus_tag="ACGVJG_002081"
     CDS             12456..>12886
                     /locus_tag="ACGVJG_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFJ6558006.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREV"
BASE COUNT         4394 a         2159 c         2762 g         3571 t
ORIGIN      
        1 tgattggcgt tcctggacga agcaatgcgt ttgaaatctc tagccgatta ggtcttgata
       61 cagaagttat tgatgaagcg aagcagttga tgaatgatga gagccaagat ttaaacgaaa
      121 tgatcacaga tttagagaat cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc
      181 attttgtttc tgaggcacaa gaacttcacg atgatttgaa agaagcttac agctatttct
      241 ttgaagaacg tgaaaaagaa atggaaaaag caaagaaaaa agcaaatgaa gtcgtgtcag
      301 aggcggaaga aaaagcagaa aaaatcattg ctgatattcg taaaatgcaa cagcagattg
      361 ggcaaggaaa tgtgaaagag catcagttga tcgatgcaaa gacccaatta gcaaatctac
      421 atcaagaaga aactttgaag aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt
      481 tgaaaccggg agacgaagtg ttggttacga cttacggcca aagagggacg cttttacgta
      541 aaaacgggaa tcagtggcaa gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg
      601 agctgacgcc tgttgctcct caaaaagagc cgacacaacg agttatccat gctgtacgat
      661 ctgaatctag tagtcacgtg ccgaatcaat tggaccttcg aggaaaacga tacgaagaag
      721 cgctaagtga ggtagatcaa tatttggatt ctgccattct agctggttat cctcaagtga
      781 ctattgtcca cggaaaagga acaggcgcac ttcgcaaggg aattactgat tatcttaaaa
      841 accatcggag cgtcaaaagc tttgagtttg caccggcaaa ccaaggcgga aatggtgcga
      901 caattgttaa atttaagtaa agcttactaa aggtaagcag atggttcgct atccaagtat
      961 agaaatgcta tactagtcat agattttaaa aatggaggtc tttgatatgt cacaagtaat
     1021 tacagataag acatttaatg aagaaacaga taaaggactt gtattgattg atttttgggc
     1081 aacttggtgt ggtccatgtc gtatgcaagc accgattttg gatcagttgg aacaagaata
     1141 tgatgaagaa gaattcagaa ttgctaaaat ggatgtggat gaaaatccag aaactcctca
     1201 acaattcggt atcatgagca ttccaacatt aatgttgaaa aaagatggtc aagtagtaga
     1261 aaaagctgtt ggtgttcatt caaaagaaca actacgtcaa atgatcgatc aatatttata
     1321 aaaacttaca gcaaaaataa gctgagggtg ggactgaatc cagttccgtc ctcggttttt
     1381 ttgcggttga gttaaggtta ttcaaattaa tattttttga gtataattac gttgtcaggc
     1441 tggattgttt ttttgacaga catgctataa taactttaat tgaatatgaa ggggagtacc
     1501 agatgaacga acgaatcaaa aacaaattag ctttacttcc tgatcagccc ggctgttact
     1561 tgatgaaaga taaaaatgga acgatcattt atgtcggcaa agccaaaata ttaaaaaatc
     1621 gtgtccgctc atattttact ggtagccata atacgaaaac ggaacggctt gttagtgaaa
     1681 tcgaagactt tgaatacatc gttacagaat cgaatatcga agcattgctc ttagagatca
     1741 atctgataaa gaaaaatgat cctaaataca acattatgtt aaaggatgat aaaacctatc
     1801 ctttccttaa gataacgaat gaaaaatatc cccgtttggt gattactcgt aaggtactaa
     1861 aagacaaagc actgtatttt ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga
     1921 agatcctcga tcgattattt cctctgagaa aatgcaaacc tagtcagacg aaggagcctt
     1981 gtctttacta tcatttggga caatgtctgt gtccttatta tttcgacgta gatccgaaag
     2041 tctatacgaa aatcgttgat gaagtgaaaa actttttaaa cggaggacac gaaaaaatcg
     2101 agtcagagat ccatcagaaa atggaaaaag ctgctgaaaa catggagttt gaaaaagctg
     2161 cagaataccg agaccagatc cgtgccattg agacaatcat gactcgccaa aaaatgacaa
     2221 atactgattt actggatcga gatgtatttg gttatgcgat tgataaagga tggatgtgtg
     2281 tccaagtgtt tttcgtaaga caaggaaagc taattgaacg agatgtttca atgtttcctt
     2341 tctataacga agcagaagaa gatttcttga cttacctcgg tcagttttat caggaaaatg
     2401 agcatttcat tcctaaagaa gtcttgattc ctgatacgat cgacaagccc agtgtagaag
     2461 ctttgttagc tacgaaagtg ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat
     2521 tggcgagtaa gaatgcctca gttgctttga atgaacggtt tgatctgatt gccagaaaac
     2581 aggaacggac gatcggagca gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa
     2641 tacgtatcga gtcttttgat aactctaata tcatggggac gaatcctgtg gctgcgatgg
     2701 tcgtattcat tgatggaaaa ccggcaagaa aagaatatcg gaaatataaa ataaaaacag
     2761 tcaacggacc ggatgattat gcatcaatga gagaagtcat ctatcgcaga tattcgcgag
     2821 tgattcgcga agggttgcct ttacctgatt tgatcttgat cgatggagga aaaggacaag
     2881 tagatgtggc aaaagatgtc ttagaaaatc aattaggaat cgatattcct attgctggca
     2941 tggcaaaaaa cgataaacat aaaacaaatg agttgctatt cggtacagat ttggctgtag
     3001 taccgcttga gcgaaattca ccagagtttt tcttactcca gcgaatccaa gatgaagtcc
     3061 atcgattcgc aatcactttc catcggcagc tacgcagtaa aaacagtttt gcttcccgat
     3121 tagatgaaat tgaagggtta ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat
     3181 cgttgaaaaa tatcacagca gccacgacag aagaattaca agaaatcgga ctgccgaaaa
     3241 atgtcgcaca aaacgtattt gataaattac atcaggaatg aggaaagggc actttacaaa
     3301 gaaagtaaag tgtcctatca ttttatgtac aacaaaaaga gcgtttacgt tttgttggta
     3361 aataataaat cgcttacgtc tggtgtctaa ggatatatgt gatttcttga agaaggagtg
     3421 aagaaatgtt agaagtcaag gatcttgtta aaacttttgg gaactacaca gctgtcgatc
     3481 atgtctcttt ccaaatcccc gatggaaaaa tcatggggct aattggccaa aacggggctg
     3541 ggaagacaac gacttttcgt ttgatcttgg attttttgac acaagaccaa ggagaagtgt
     3601 tatggaatgg ccatcatctt ggggagaaag attatgatgt catcggttat cttccggagg
     3661 aacggggact ttatccgaaa gtaacgattc aagaccaact gctttatttt gcagaactta
     3721 gagggaaatc aagaaaagag attgaaccaa aaatcgactt ttggatggaa aaatttcagg
     3781 taaaagggaa aaagacggat aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc
     3841 agctgatcgc cacgttaatc catgaaccta aattgatcat tttggatgag ccattcagcg
     3901 ggttagatcc ggtaaatgct gaattgctaa aagatggaat attggaattg aaaaaacatg
     3961 gttcatgtgt cattttttct agccacaata tggataatgt agaaaaaatc tgcgatcatt
     4021 tgatcatgtt gcgaaacgga aaaatggttt tgaatggaaa agtacatgag attcgtgaat
     4081 catttggccg tacgaagtta ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa
     4141 tcgaaggcgt gaaaaaagtg atccttcatg aagacaatac gttagagatt accttagaag
     4201 atcctgaagc tgggaaagaa atctttactc gtgcgactca gttcggctac attcctatgt
     4261 ttaaccagca accgccaact ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg
     4321 cgtaagtttt ggattattac caaagatgtc tatttaaaaa atgttaagtc gatttctttc
     4381 ttgatcatga tattagtccc ttttatcatg ctgggtatca tttatgtagc aggaaacttt
     4441 gctcaacaaa atagtgatgt caataaaatc ggcgtattgg ctgataatga gcaaatcagt
     4501 cagcaaatga gccaagtcaa aacagatgat tttgagttca aagcagttga ttctgaagaa
     4561 aaagcacaaa aagagctttc ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag
     4621 aatgataaga taaatgggat gctttatagc gaaaattcat taggacaaag tacacaacta
     4681 ctgatacaac aacaactgac tggcttgcaa tcgatgcttc gagcgagtca attaggcatt
     4741 tcaccagaag aagtagctag cttgagccaa caagcaagtt tgactcgaca aaaagtcaat
     4801 tttgattcaa aaggaaaaat gacagtcgga gaagataact cgacagtcca atacgtcgta
     4861 agttatgtag caacgattgt cttgttcatt attattttga cgtatgcgca gatcattgca
     4921 caagagatcg cttctgaaaa aggcacgcgg atcatggaag tgatcctgtc cagtacgact
     4981 gcacaaaagc atttttacgg aaaattaaca ggtgttttac ttgttgcatt aacgcaaatg
     5041 gcattatacg gtattatttt tggtatagga tacagtcaat tcaaagatat ggaaatggtc
     5101 aaaaatatat tagacggtat ctcattggat aacttgattg gaccattctt gtggtattct
     5161 cttctattca tgatctttgg cattttgatt ttttctgttt tagcagcttt atgtggctct
     5221 ttggtcaaca aagcagaaga tacagcaaaa gcaattttgc cagtgactta tctatctcta
     5281 ggcggataca tgcttggatt gatactagga gcttcagatc caaacaatat cgttattcgg
     5341 atcacttcgt atattccatt cttatcttca tatattatgc cggttcgctt agcaaatgag
     5401 acagtcggta tgggtggagc attgatttcc ttgttggtac ttgtagccgt aacattctta
     5461 ttgatgatct tgtctgcaaa catgtacaaa tcgaatgtac tcgtttatag cgaaggcgga
     5521 ttatggagtt cattgaaaca atcgatttca attatgagga atgaacgaaa aaaagcataa
     5581 aacaatccta aaatacagaa ggaagctgga catctattgt tcccagcttc tttttgtata
     5641 gacctatttt atttatttta ctatattgga tatatattca ttgaaaatag tgtataacaa
     5701 ctttataata ttgagtatac caatttttgg aggcgtttta atggaccgtt atttagattt
     5761 actttcagaa aaattcccct caacggagga tgtcatcacg gaaatcatca atctcgaagc
     5821 aattttgcag cttccaaaag gaacagagtt atttttgagt gatatacatg gcgaatttcc
     5881 tgcttttgat catattctgc gtatcggttc aggaaatctt aaagaaaaga tcaaagacct
     5941 tttttctgaa caaatgacgg aaacggatat cagccaattc actattttta cggcttatcc
     6001 agaatatgca ttaacgactg attggtacaa agaacaagat aaatcgcagc tgatccatca
     6061 tttgatcgat cttttacggt ttacgacagt caaatatacc cggtcaaaag ttcgaaaagg
     6121 attgccgaaa gaatatagct atatcattga ggaactcttg tatatcgatg accgtatcaa
     6181 tggaaaaaaa gactatgtaa aaaaaatcat tgaacaactt gttttgatga aggaagaaga
     6241 aaaattttta acgaaacttg cacagacgat ccaaaaatct gtcatcgatc atttgcatat
     6301 cgttggagat atttttgacc gaggaactca agcagccaaa gtgatggatc gaatcatgga
     6361 tttttcttct gtagatatcc aatggggaaa tcatgatatt ttgtggattg gtgcgtattg
     6421 cggctctgag gcatgtttac tgacactttt acggattgca gcacgctata attatttatt
     6481 tgaattagag aaagaatatg ggttgaattt acgtccattg ttttcttttg ctcatcaaac
     6541 ttatcagaag aatccagtat ttgcgcctaa aaacagtaaa aatctatccg aaagagagca
     6601 agaagaatta gaaaaaatcc atcaggcact ttctatcctt caattcaaat cagaacatca
     6661 gttattggat cgtcgacctg aatttaaaat ggacgatcgt aaattgctgg aaaaaatcga
     6721 ctatgaagca tcaacgattg atttagaagg tcagctctat gagttgaaag gaacttgttt
     6781 tcaaacgatc cagccagatg atccaaaaaa attcttggaa gaggaacaaa aagtgattga
     6841 tagtttgatg tattcattcc agcactcttt gagaatgcaa aaacatatga cgtttttgat
     6901 cgaaaaaggg ggaatgtatc tcacgaacaa tcagcatgtg ctgtttcatg gctgtatacc
     6961 tgtagacgag aagggacagc tcttagcatt tgaattagac caatcttatc gcggaaaaga
     7021 actgctgggc ttttttgaaa aacagataac tgaaagcgca aaagatctga ctgcaaaaga
     7081 agatttagca accgatttga tttggtatgc gtggagtggg ccattttcgc ctttattcgg
     7141 caagagtaaa atggcgactt ttgaacgtta ttttctcaca gagcctcata cgcacgtcga
     7201 gaaaagcaat ccttattatc acttgcgaga tgaagagtgg ttcagtgaaa aaattttagc
     7261 agaattcgac acaaaagaag aagggtcagc aatcattaat gggcacacgc cagtaaaagt
     7321 aaaaaaaggg gagtcaccga tcaaagcaaa tggaaaagta tttgtcattg acggaggttt
     7381 atccaaagct tatcaaaaaa cgacaggaat tgccggttat tcactattat gcaattctta
     7441 tggtttccaa atcgtgacac attatccgtt tttgcctatt gaacaacaat ttgcaaaaaa
     7501 atccgaccaa acaaatgtga aaaaagtaat cgaacagcaa ctaccacgca aactcaatcg
     7561 tgatacaaca aaaggtatgg aactgcagca gcagattacc cagctcaaaa ggctgttgga
     7621 ctatcgaaag gacgattgag aagaattcca gtgaacattg ttccatagac tggagactga
     7681 gacaaaagtc gtgtcgcagt ctctttttcc ggataaacgg tgttcttctg ccaactcctc
     7741 ggtcacaagt cacactattc aacaaacatg aaaagctgtt ttttgaaatt gctccttgtg
     7801 actcagagtt gaacggcagg ctcacgacct cttggtataa acggtgttca aaagccagtt
     7861 tctcggtatt aagccaaatt tatgcaaaaa tgtgaaaagc cattttcaca aaatttttct
     7921 taatactcga aactaaacag ctttttcaca accccctcag aaagtctatt aaaattcatt
     7981 cacaaagaaa tcgaataagc gctgttcaga tggatgtaca cggtgggtaa gttccggatg
     8041 ccattgaaca ccgaggattt tatgcatatc atctttgctt tgaaccgctt caatgagtcc
     8101 atcgttagaa aaagcgatgg cttttaataa aggtgaaaga tctttgactg cctgatgatg
     8161 gtaagaattc acaaaatatg gatcggggag aaggttgctt aacaaactgt ctgaaacaat
     8221 cttgacttcg tgagtcgtaa attgcggggc agtgggctgt tgttcatgtt ttactgacca
     8281 ctttggatat aatgaaagat cttgataaag tgtaccttcc aaaacgacat tcaatagttg
     8341 gagtccccgg cagactgcaa aaattggttt tctttgtttc aatgcttcca agatcagtgc
     8401 ctgttcaaat tggtctcgtt gaatatttgt ttccgttagc tttggatgag gatcttccat
     8461 gtaaagttgt ggactgacat cttgaccacc tgcaagaata agtttgtcga tttgtccaat
     8521 cagttgagga gctgaatctg gagacatgac tggcagaata agaggcaatc cttgagcatc
     8581 taaaactgca tccacgaatc cttgcggtgt atagctcact tgatttcctt ggaaagtatc
     8641 tgttgctcga atcagttgat ttccggcaat gccgatgatt ggtttcattt gtattccccc
     8701 taaagtagac ttattgtaac aaggatttaa aaaaaattaa actatgtaag acttgcccct
     8761 atacttatct gttcttgctg tgttactatc tgtaaagaga aatttcgtgt gaaattcatg
     8821 acgatagttg aaaatattgc ttaatttgtt ataataaaaa gtaagcagaa ataaaaaggt
     8881 tgggcgtaag cttcaaggat ctagttcttc aaggggtgag aaggacattt tattgaaggg
     8941 gcctgcctat actaagttat ctttagaggt acatactttc aatgaaaaaa gaaagcatgt
     9001 acctcttttt taaatcgaaa ggaagtgaag caagtgttag aagctttgga acaaatccga
     9061 aaagcagaag aagaaaatga aaagcgtgct caaacactgc ggcagcagct gaagatctat
     9121 gcaacagaaa aagagcagga attggaaacg atccaaacaa actatcgaca acaactaaaa
     9181 cagttggtgg aagaaaaaga acaatttgaa atacaagaat tagaaaaaga aaaaatgaaa
     9241 ttgaaagcag aagcagacaa ggtgcaagca actttgaaag atcaatatca aaagcatcag
     9301 agtcaagcga tcggtgcgat cattgaaagg gtgaaagaaa cttatggccg tcactaagat
     9361 ggaaaaagtg accctcattt cagataaaag aaatcaggag aggattttac aagcagtaca
     9421 aggaatccaa aacgttgaaa tccgggatct gtatcaaaat agaacaaaca accagtgggt
     9481 ggagaagtat tttccagata ccacgattat tgataaagaa gcaaaattaa gcgctttagg
     9541 taatcggttg aaagacatcc aagaagctat ccaattcata caacaccacg gcgatagccg
     9601 tcaaaagaaa ctccatttga aacgtcaaga actttcctta caagatttgg aagccaatta
     9661 ttccgaagaa aatttcataa ataaattgaa agaagtcctt gatttaaaag aaaagtggga
     9721 acaactaact gaagaacgtg atcgtttaga agaagaggaa gaatggcttc ttcattggca
     9781 acagctggat attgctccaa gtatgtatgc aagtaagacg accatttttg agatggggac
     9841 tgtgagtgca gcaaattttg aaagtttcaa ggaagaactg agtaagcttg atgacgttta
     9901 tatggaagaa atagattata cgccaaaaca agtttatctt tcgtatgttg ctttaaaaaa
     9961 atcagttgct gttatagaag aaattgctgg gaaatacggg ttcaataaag aaacgtatcc
    10021 ttatgaacaa aatcctaaag aaaaactgca agagaccaag cagcaattat ccattgttca
    10081 cgagcagcaa aagaagataa cgacagcgtt aggtcagtgc agtggctata tccaagaatt
    10141 tgaatgggta gaagaagtca cacttgccgt tgcagaacga gaaaaaataa aagaacggtt
    10201 tatccatgcg acttatctga tcgttttgca aggttgggta gatgcagaag aaaaacagaa
    10261 tttgctgcat gtgttaaacg aaaccgtatc agaagaagat atatacgtca gtttcgaaac
    10321 accaaactca gaagagattc agcaggaagt ccctactaaa ttgaaaaacc atccgcttgt
    10381 cgaacctttt gaattgctga ctgaaatgta cagtctgcca aaatatgaag aagtagatcc
    10441 aacaccttgg atgacaccat tttatcttgt cttcttcggg atgatggtcg cagatgttgg
    10501 ttatggtttg ctgatgctca ttgggacaat actagctcaa aaactacttg ttctaccacg
    10561 cgggatgaaa cgtttcgtaa aattctttga gattctctcg ataccatcga tcatctgggg
    10621 attgatctac agttcatttt tcggacagtc attaccaaaa gaactgtttg gcatccgctt
    10681 gcctttccca atcctatcta cgacggatga cgtcaatacg attttgattt tatccgtgat
    10741 tttcggtctg atacagattt tggttggatt atttgtggca gcaaaggaaa atatcaggag
    10801 aaaagattac ttgagtgctg tcagtgatgg atttgcctgg caagggatcc taattgggat
    10861 cgttattgcg ttggttggtg ccatgttgat aaaaaataca gcatttgttt accttggcgc
    10921 tgggctatcg atcattgctg ccttatgtat cttgatcgtt ccgattatcc aaacgccgtc
    10981 taaagtcaaa ggtgctgcca aaggtgcgta taatctttac ggactgaccg gctatatcgg
    11041 tgatttagtc agttatacac gtttgatggc attgggtatt tcaggcggaa gtattggagc
    11101 ggcattcaat atgctagttg ctttcatgcc gccagctgct cgattcagtg tagggatcct
    11161 tttgatcatc gctttacacg cactgaatat gtttttgaca ctgctaagtg cctatgtcca
    11221 tggcgcacga ttgcagtatg tcgaattttt tgggaagttt tatacaggag gcggacgtgc
    11281 ctttgatccg ctgaaaacgg cggaaaaata cgtcaacatc aatcacaaga aaaaaaacaa
    11341 taaataattg gaggattttt atataatgat ggattactta attagtcaaa atggtggaat
    11401 ggtatttgcg gtattagcga tggcaacagc gacgattttt tcagggatcg gttcagctaa
    11461 aggtgttgga atgacaggtg aggcagcagc agcattgaca acaagtcaac ccgaaaaatt
    11521 cgggcaggct ctgattctgc aattgcttcc aggtacgcaa ggtttgtatg gattcgttat
    11581 tgcctttttg atttttatca acttaggaaa tgacatgtca gttgtacaag gactgaactt
    11641 tctaggtgct tctctgccga ttgcttttac aggcttgttt tcaggtattg cccaaggaaa
    11701 agtcgcagcg gcaggtattc aaattttagc gaaaaaacct gaacatgcta caaaaggaat
    11761 catttttgct gcgatggttg aaacatacgc gattttaggt ttcgttattt ccttcctatt
    11821 agtgttgaat gcctaagcat aaaggaggaa aagcagatga acgccattga aaaaatcatc
    11881 tctcagatga acgaagcagc agagcaggaa agagcggcgt tagaacaaga agagcgaatg
    11941 aagatcgatc aaaactttga acaaaaacgt acgcaagttg aaacagaaca tcaaaaacaa
    12001 aaagaaaaac aaatcgaatt actcgaaaaa aaataccgtc agctaaggaa tcgccagcaa
    12061 gtagaagtac gacaagaaaa tttgaatgcg aagcaggaat ttttaagacg attgtttgca
    12121 gatgcggtaa ctgaaatgga aaattgggat gaatctgaac aaatccagtt tatcaaaaat
    12181 gcattgtatt cattaccttt gacaggaaaa gtagcattca ttgctggaga aaaatcagca
    12241 gcctatcttt ctcagacgtt gttggatgaa tggaacaatg aacttccctt tatgatggtt
    12301 ttaagtgatg aaactgttgc tgatcaagcg ggttttctga taaacgatca aggagttcag
    12361 tataattttc ttttcagcag ccttgtacaa gatatccaag gaacgatgag ttttgagatt
    12421 gccaatcaat tattcgaata aggagggatc agtgtatgga ataccacgaa ctaaatccct
    12481 tgattcgcgg aagagagctt gaacttttgt ccaatgagac atttgaacgg atgattcaaa
    12541 caaattctgt cgaagcactt ggtgaaatat tgaaatccac gatttacagt ccatatattt
    12601 atgacggata tgaatatgac ttcgaacaga atcttttaaa ggagcagagc cgtctatttc
    12661 gctggttaaa ggaatcagca ccggagcctg aaatcgtttg gatctatacg atgcgttata
    12721 ctttccataa tctgaaggtt ttgacaaaag ctgagatgac aaatcaaaat ctggaccatt
    12781 tgtatatcga cgatggattt tattcattag aaaccttgaa ggatgccatt catacgcaag
    12841 tttcaaatga acttcctgaa agcattatgg acagtatacg agaagt
//
LOCUS       DAYLZO010000080        12832 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.081, whole genome shotgun sequence.
ACCESSION   DAYLZO010000080 DAYLZO010000000
VERSION     DAYLZO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.081"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            185..1183
                     /locus_tag="ACGVJG_002082"
     CDS             185..1183
                     /locus_tag="ACGVJG_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFJ6558007.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACGVJG_002083"
     CDS             1295..1804
                     /locus_tag="ACGVJG_002083"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFJ6558008.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACGVJG_002084"
     CDS             complement(1834..2493)
                     /locus_tag="ACGVJG_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFJ6558009.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACGVJG_002085"
     CDS             complement(2495..3259)
                     /locus_tag="ACGVJG_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFJ6558010.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACGVJG_002086"
     CDS             complement(3267..3944)
                     /locus_tag="ACGVJG_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFJ6558011.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACGVJG_002087"
     CDS             complement(3981..5366)
                     /locus_tag="ACGVJG_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFJ6558012.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACGVJG_002088"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGVJG_002088"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACGVJG_002089"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACGVJG_002089"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFJ6558013.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ACGVJG_002090"
     CDS             6305..7162
                     /locus_tag="ACGVJG_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558014.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ACGVJG_002091"
     CDS             complement(7243..7578)
                     /locus_tag="ACGVJG_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558015.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ACGVJG_002092"
     CDS             complement(7645..9015)
                     /locus_tag="ACGVJG_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFJ6558016.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ACGVJG_002093"
     CDS             complement(9093..9629)
                     /locus_tag="ACGVJG_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6558017.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="ACGVJG_002094"
     CDS             9938..11419
                     /locus_tag="ACGVJG_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558018.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="ACGVJG_002095"
     CDS             complement(11522..12229)
                     /locus_tag="ACGVJG_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFJ6558019.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="ACGVJG_002096"
     CDS             complement(12308..12646)
                     /locus_tag="ACGVJG_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFJ6558020.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
BASE COUNT         3909 a         2609 c         2085 g         4229 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgata tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaataaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aa
//
LOCUS       DAYLZO010000081        12764 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.082, whole genome shotgun sequence.
ACCESSION   DAYLZO010000081 DAYLZO010000000
VERSION     DAYLZO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.082"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACGVJG_002097"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACGVJG_002097"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFJ6558021.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACGVJG_002098"
     CDS             complement(2262..2513)
                     /locus_tag="ACGVJG_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFJ6558022.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACGVJG_002099"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACGVJG_002099"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFJ6558023.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACGVJG_002100"
     CDS             3463..3681
                     /locus_tag="ACGVJG_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558024.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="ACGVJG_002101"
     CDS             complement(3970..4482)
                     /locus_tag="ACGVJG_002101"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFJ6558025.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACGVJG_002102"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACGVJG_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFJ6558026.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="ACGVJG_002103"
     CDS             complement(6935..7378)
                     /locus_tag="ACGVJG_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFJ6558027.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="ACGVJG_002104"
     CDS             complement(7379..8167)
                     /locus_tag="ACGVJG_002104"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFJ6558028.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACGVJG_002105"
     CDS             complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACGVJG_002105"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFJ6558029.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="ACGVJG_002106"
     CDS             complement(9282..9869)
                     /locus_tag="ACGVJG_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFJ6558030.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACGVJG_002107"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACGVJG_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFJ6558031.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="ACGVJG_002108"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="ACGVJG_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFJ6558032.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
BASE COUNT         3528 a         2859 c         2269 g         4108 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttc
//
LOCUS       DAYLZO010000082        12740 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.083, whole genome shotgun sequence.
ACCESSION   DAYLZO010000082 DAYLZO010000000
VERSION     DAYLZO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.083"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACGVJG_002109"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACGVJG_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFJ6558033.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="ACGVJG_002110"
     CDS             complement(324..893)
                     /locus_tag="ACGVJG_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFJ6558034.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="ACGVJG_002111"
     CDS             1079..1981
                     /locus_tag="ACGVJG_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFJ6558035.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACGVJG_002112"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACGVJG_002112"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFJ6558036.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACGVJG_002113"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACGVJG_002113"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFJ6558037.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACGVJG_002114"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACGVJG_002114"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFJ6558038.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="ACGVJG_002115"
     CDS             8549..8734
                     /locus_tag="ACGVJG_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFJ6558039.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="ACGVJG_002116"
     CDS             complement(8796..9992)
                     /locus_tag="ACGVJG_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFJ6558040.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="ACGVJG_002117"
     CDS             complement(10162..11598)
                     /locus_tag="ACGVJG_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFJ6558041.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="ACGVJG_002118"
     CDS             11891..12700
                     /locus_tag="ACGVJG_002118"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFJ6558042.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3805 a         2673 c         2249 g         4013 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatga
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtgacaattt
//
LOCUS       DAYLZO010000083        12740 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.084, whole genome shotgun sequence.
ACCESSION   DAYLZO010000083 DAYLZO010000000
VERSION     DAYLZO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.084"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            99..542
                     /locus_tag="ACGVJG_002119"
     CDS             99..542
                     /locus_tag="ACGVJG_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFJ6558043.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(548..1732)
                     /locus_tag="ACGVJG_002120"
     CDS             complement(548..1732)
                     /locus_tag="ACGVJG_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFJ6558044.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2000..2698
                     /locus_tag="ACGVJG_002121"
     CDS             2000..2698
                     /locus_tag="ACGVJG_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFJ6558045.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2705..3382
                     /gene="nth"
                     /locus_tag="ACGVJG_002122"
     CDS             2705..3382
                     /gene="nth"
                     /locus_tag="ACGVJG_002122"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFJ6558046.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3508..4041)
                     /locus_tag="ACGVJG_002123"
     CDS             complement(3508..4041)
                     /locus_tag="ACGVJG_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558047.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4338..6719)
                     /locus_tag="ACGVJG_002124"
     CDS             complement(4338..6719)
                     /locus_tag="ACGVJG_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFJ6558048.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6709..7356)
                     /gene="recU"
                     /locus_tag="ACGVJG_002125"
     CDS             complement(6709..7356)
                     /gene="recU"
                     /locus_tag="ACGVJG_002125"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFJ6558049.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7420..7959
                     /locus_tag="ACGVJG_002126"
     CDS             7420..7959
                     /locus_tag="ACGVJG_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFJ6558050.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8049..8468
                     /gene="gpsB"
                     /locus_tag="ACGVJG_002127"
     CDS             8049..8468
                     /gene="gpsB"
                     /locus_tag="ACGVJG_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFJ6558051.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8490..8857
                     /gene="rnpB"
                     /locus_tag="ACGVJG_002128"
     ncRNA           8490..8857
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGVJG_002128"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9066..10232
                     /locus_tag="ACGVJG_002129"
     CDS             9066..10232
                     /locus_tag="ACGVJG_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFJ6558052.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10278..11774
                     /locus_tag="ACGVJG_002130"
     CDS             10278..11774
                     /locus_tag="ACGVJG_002130"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFJ6558053.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11855..12538
                     /locus_tag="ACGVJG_002131"
     CDS             11855..12538
                     /locus_tag="ACGVJG_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFJ6558054.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12547..>12740
                     /locus_tag="ACGVJG_002132"
     CDS             12547..>12740
                     /locus_tag="ACGVJG_002132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558055.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4273 a         2230 c         2630 g         3607 t
ORIGIN      
        1 tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg aataggaaac
       61 aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga caactgctaa
      121 tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca gaaatggaaa
      181 aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc gggaaaatcc
      241 ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga gccatcagta
      301 agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc gaagagaata
      361 aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat agacacgagc
      421 aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg tcaaaagaag
      481 aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga agtagcaaat
      541 gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa aatccgaata
      601 ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg ccgatgacgc
      661 gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg ttatctgaga
      721 cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg aaaccataac
      781 agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat gtgatgaata
      841 gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct gaaaattttt
      901 tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca atcaaagaga
      961 agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat tgagaaaaga
     1021 tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat aaaggaataa
     1081 cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg gagaaactta
     1141 aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag ccataaacgg
     1201 catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc aactcagcta
     1261 atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca cccatccatc
     1321 caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca tataaggtgg
     1381 agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc catatcgaat
     1441 ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata ataaggatcg
     1501 gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag aaggcagcta
     1561 gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg gagtgagaat
     1621 aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat ccagtgaata
     1681 agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac atgtaaaaac
     1741 tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt ttgtttcctt
     1801 gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa agtgaagata
     1861 cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc tgaccgatca
     1921 gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac gtatactgtg
     1981 attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg gacaaacagt
     2041 catatctaat cttttactta aaaattatca taggatcggt ctacaatctg aagaattttt
     2101 atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg atttgcagat
     2161 gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg atcgtctcgt
     2221 aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag cagatcgata
     2281 taatctttac cccatctatg atctattagg tgagtattta aaaacacaag aaaaaaaata
     2341 cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg aacaagaatt
     2401 tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag aagaagacca
     2461 ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc aggcttatag
     2521 ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac gtacaaaaca
     2581 acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag aagtagaaca
     2641 gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg aggagtagcg
     2701 gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg gaatgtttcc
     2761 cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg cagtgatctt
     2821 aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt ttgcctcttt
     2881 tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta aaataaaaac
     2941 gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc agctgattga
     3001 acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc ctggtgtagg
     3061 cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa tcgcagtaga
     3121 tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg ctacggtcat
     3181 ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa agacacatca
     3241 tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt gcgaggtatg
     3301 tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag aaaaggcgct
     3361 aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg tatgaattat
     3421 cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa gtaaatgtac
     3481 atactcagta gctaggatgg taacttatta caatttattg cgataaaaat aaaagttgtt
     3541 atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac ctataaaata
     3601 agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa
     3661 aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg
     3721 cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag
     3781 aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac
     3841 agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca
     3901 aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg
     3961 gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt
     4021 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
     4081 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
     4141 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
     4201 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
     4261 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
     4321 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
     4381 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
     4441 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
     4501 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
     4561 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
     4621 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
     4681 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
     4741 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
     4801 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
     4861 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
     4921 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
     4981 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     5041 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     5101 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     5161 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     5221 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     5281 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     5341 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     5401 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     5461 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     5521 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     5581 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     5641 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     5701 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     5761 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     5821 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     5881 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     5941 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     6001 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     6061 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     6121 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     6181 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     6241 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     6301 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     6361 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     6421 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     6481 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     6541 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     6601 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     6661 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     6721 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     6781 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     6841 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     6901 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     6961 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     7021 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     7081 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     7141 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     7201 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     7261 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     7321 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     7381 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     7441 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     7501 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     7561 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     7621 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     7681 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     7741 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     7801 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     7861 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     7921 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     7981 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     8041 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     8101 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     8161 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     8221 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     8281 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     8341 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     8401 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     8461 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     8521 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     8581 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     8641 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     8701 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     8761 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     8821 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     8881 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     8941 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     9001 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     9061 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     9121 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     9181 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     9241 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     9301 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     9361 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     9421 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     9481 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     9541 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     9601 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     9661 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     9721 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     9781 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     9841 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     9901 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     9961 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
    10021 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
    10081 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
    10141 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
    10201 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
    10261 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
    10321 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
    10381 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
    10441 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
    10501 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
    10561 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
    10621 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
    10681 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
    10741 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
    10801 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
    10861 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
    10921 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
    10981 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
    11041 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
    11101 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
    11161 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
    11221 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
    11281 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
    11341 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
    11401 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
    11461 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
    11521 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
    11581 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
    11641 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
    11701 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
    11761 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
    11821 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
    11881 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
    11941 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
    12001 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
    12061 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
    12121 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
    12181 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
    12241 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
    12301 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
    12361 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
    12421 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
    12481 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
    12541 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
    12601 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    12661 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
    12721 gcaggagaac accgtttatt
//
LOCUS       DAYLZO010000084        12350 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.085, whole genome shotgun sequence.
ACCESSION   DAYLZO010000084 DAYLZO010000000
VERSION     DAYLZO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.085"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            121..690
                     /locus_tag="ACGVJG_002133"
                     /pseudo
     CDS             121..690
                     /locus_tag="ACGVJG_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1091..1696
                     /gene="fic"
                     /locus_tag="ACGVJG_002134"
     CDS             1091..1696
                     /gene="fic"
                     /locus_tag="ACGVJG_002134"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFJ6558056.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1712..2284
                     /locus_tag="ACGVJG_002135"
     CDS             1712..2284
                     /locus_tag="ACGVJG_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFJ6558057.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2536..2793)
                     /locus_tag="ACGVJG_002136"
     CDS             complement(2536..2793)
                     /locus_tag="ACGVJG_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558058.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2919..3734)
                     /locus_tag="ACGVJG_002137"
     CDS             complement(2919..3734)
                     /locus_tag="ACGVJG_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558059.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4251..5576
                     /locus_tag="ACGVJG_002138"
     CDS             4251..5576
                     /locus_tag="ACGVJG_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFJ6558060.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5569..5919
                     /locus_tag="ACGVJG_002139"
     CDS             5569..5919
                     /locus_tag="ACGVJG_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558061.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5901..6086
                     /locus_tag="ACGVJG_002140"
     CDS             5901..6086
                     /locus_tag="ACGVJG_002140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558062.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6990..7265)
                     /gene="prgO"
                     /locus_tag="ACGVJG_002141"
     CDS             complement(6990..7265)
                     /gene="prgO"
                     /locus_tag="ACGVJG_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFJ6558063.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7237..8190)
                     /locus_tag="ACGVJG_002142"
     CDS             complement(7237..8190)
                     /locus_tag="ACGVJG_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6558064.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8802..10295
                     /locus_tag="ACGVJG_002143"
     CDS             8802..10295
                     /locus_tag="ACGVJG_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFJ6558065.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10430..10726
                     /locus_tag="ACGVJG_002144"
     CDS             10430..10726
                     /locus_tag="ACGVJG_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFJ6558066.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10827..11678
                     /locus_tag="ACGVJG_002145"
     CDS             10827..11678
                     /locus_tag="ACGVJG_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558067.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11699..11911
                     /locus_tag="ACGVJG_002146"
     CDS             11699..11911
                     /locus_tag="ACGVJG_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558068.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4243 a         1895 c         2309 g         3903 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 acctattatt ttaagtctga aaaggataca tcgtctctaa cgttaacttt agaggaagga
      181 gttgaacctg acaaggtgac ctctaaagtg ataaaccaat tgaaagaaaa aggtagtttg
      241 aaacatttgg gggaatatga agtgttagat acggctatgt taacgaaagg aattggtcaa
      301 atcctcagta cgatcactta ttttataaca gctgtagcag gtatatcttt atttatcgca
      361 ggggtaggtg taatgaacat gatgtatata tctgtatctg aacgaacaaa agagatcggt
      421 ataagaagag ccctaggagc tactcgaaaa tcgattatgc tgcagttcct tttagaaggt
      481 ttgattttaa ctctttcagg agggttaatt ggctatttat taggaatggc tcttgcttat
      541 agtgttggtt ctttgataaa ggtaaacgtg tcacttgatc tatttactat tttactagcg
      601 gttggggtat cttcggttat tggactggtc ttttctgtaa tgcccgcttc agaggctgca
      661 aaaaaagatt taattgatat tcttagataa aatcagaatg acaagaatat ttacgttcta
      721 gattctaaaa taaaacacaa agagaccaac caggcaccct attgtttctt tgtagtatag
      781 agatattctt tggcttgtaa gagatcaata gagttaggtg accggagttc gaaataaagg
      841 aaatttttga ttgtatagga gaattcttta tccctaatat ttagaatttt gagtatgcca
      901 tgttgatgtt ttagattctc tataaatata gaggtgtatc aaaggtcaac tagactgtgc
      961 aaaatagaga cacttgaaaa atctatcggg ggtcagaaac ttaatagttt ttgacacata
     1021 aacaaaatcg cgatataatg gggttgagag tgtttcataa acttatgtca taaagttaag
     1081 gagatttcgc atgattttag agaataaatt gggattaacc aatcaagttg agttggctaa
     1141 ggttgaagaa aaactcagta aacaaaaagc gaaagaattg tacgattgtg gaaaaataaa
     1201 tgaaatcgaa gttggtacat ttaaaggttt atctgaaatt catgaatttc tattttcgga
     1261 tatttacgat tttgccggaa aaatccgatc ggtcaatatt gcaaaaggaa attttcgatt
     1321 tgcaccagtt atgtatttag aacattcgtt acagcatatt gatcagatgc cacaaacttc
     1381 ttttgaagag attatcaaga aatatgtgga gatgaatatc gctcacccat ttcgagaagg
     1441 aaatggccga agtactagaa tttggctgga tctcatttta aaggaagaat tgcaaaaagt
     1501 tgtagattgg aacttaatcg ataaagcaga ctatctttct gcaatggagc gaagtcccat
     1561 aaacgatttg gaaatacgct atctaatttc caatgctctg actgataaaa ttgacgatcg
     1621 cgagttatat atgaaaggaa tcgatgtcag ctatttctat gaagggtatt ctgagtatac
     1681 gatagatgat ttgtaaaagt aggtggacca aatgacgaaa tatggatatg cacgtgtctc
     1741 aaccgcttct caaagtacaa aagaacaaat tcaacagttg cttgagaacg gcgtagagaa
     1801 gaagaatatt ttttcagaaa aatttacggg tacaaaacgg gatcggccaa aattcaatca
     1861 gctgttggct ggtctgaagc ctggagatga aatagttgtt acgaagcttg atcgatttgc
     1921 tcgtaacacg agagaagcat tagagatcat tgaaccttta ctcgatgata acgtaacgat
     1981 caaggttttg aatctaggaa cgattgaaaa tacgtccatg gggcgaatgg tgacacggac
     2041 gttgttgagt gtagcagaaa tggaacgtga tatgattgtg gaacgtaccc aagaaggaaa
     2101 aatgtttgca agaaagaata atcctaattt cagagaagga cgtccgaaag caacaatcac
     2161 accgaagaag aggcacgcat atgaactgct tacttcaggt aaatcgtata aggaagttga
     2221 agctataacc ggtttcagtc gcagtacgct ttttagaata aagaagaaaa ttgaagaaag
     2281 ttaaaaaaga agtctcagcg aaccagacag gtaaatactt aatgcgttct agatatgagt
     2341 ctattagcat gttgtgaaat ataatatttt tttatttact tgatttctgc tcaaaaaatg
     2401 tgtatggtaa ctgtaaaagg taaagtaatt aaaggtatgc tagccacgct tactatatta
     2461 ggataccatc atatatttat aatgaacgat ttgttcgtct accaaacaac acatatcgtt
     2521 cagcgtaagt cacgtttagg aacgtggctt ttttagtgtt attatcgata aataatctat
     2581 tgactctatt gattttatct ccatatccct acggtgttga ttgtctttag atctttcatt
     2641 taagaaccaa ataactatca taaaaatcag cactgctgct aatataataa acagcattcc
     2701 tattgtgata ttacctattg attttaccat aactacgatt aaagtaataa tggcattcaa
     2761 gtctcctttg ttctctatat ataaattagc cataaatcat cttcctttca aattaagata
     2821 cttgtttgat accaataaat agtaagcgtg gctagcatac ctttttctgt aatagcaagt
     2881 gttctaatag tccaaactta atgatggacc gatgctagtt atagtacagt aatgttttct
     2941 ttcttatcta gatttttagt aagctctatc agcaaatcta acaaatctga gtccttttca
     3001 gaaactaaat taaatctagt gtttacaaca aagttttcat gttctatatt gtttagatta
     3061 tttgcggaaa ggagggcttc tagatcttga cattcaaatc tatatgcatc gtcataaatc
     3121 ttactagaag gagtgcttaa ttcgaaatta cttattttct catcttggac caagataaat
     3181 ttatagatag tatctttttg tgtttttctt ttaaaaacac aacagagtgt gttataagga
     3241 acgttataat tttcatcagc ctcatttttt ctccattcaa acaaatgata aactgcaacc
     3301 aagttgccat caatttcatg gacagtgctc tcggtaacat cctttaaaga actatcaagg
     3361 gctaatttat agtaactaga gagaggaagt aaaattgtcg atttactgag atggttatcg
     3421 tctatacgtg caatcatacc taaatcaact aaggttttat tccaggtgtt tatagtttta
     3481 gttgtcactc ctagagactc tgctatggta ttgaccgaat gccaggaatc cccgccttca
     3541 ttctttgcct taaaacagta atacagatat aaattaatag ctttgttatt cggtaaatat
     3601 ttttcaagtt ctgttggtag aataaagtaa gaatccttaa tgttttttct gaactgtctc
     3661 cagtctctgt agttttgttt ataaagttgt gaaacggttt ttccaccttt agaaagtcct
     3721 gctatcttaa ccaatcttat tgctcctttc ttgaatctgt catgatcata cagtattttt
     3781 tacttgtagt ccagcacctt aatatattat cctatgtaat taatttcgta ctagatagta
     3841 attatttttt atttaattct ctagtcactt acaaataagt tatataaact acgattaata
     3901 aattattaca aaaactatta agaaaacttc tacatacaac tattacaaaa ctattaagaa
     3961 actgaatact cctatttttt gtttttaagg aaagtttttt cttttttgaa tttcttattt
     4021 ttttctaata taactaagca aaactggata atacgacact tattttcaaa ttttcacaag
     4081 actaatagta aatatccatt taataaagtg atacaatgtg ttgattttac aaatcaacag
     4141 aagaagtagg gaaataactt acctatgctt ctttttttgt acatttaatc gattcttctt
     4201 tacaaaaacg aacgtatgtt tgtataatgt ggaagaatgg agtgttattt atgatggatt
     4261 attcaaaaga gccagtaaat gattactttt tgatcgatat gaaaagcttt tacgcaagtg
     4321 tcgaatgtat agaaaggaat ttagatccat taacgactga attagtcgtc atgagtcgag
     4381 cggataatac aggatcaggg ttgattttag cttcttcacc agctgcaaag aagaagtacg
     4441 gtatttctaa tgtcagtcga ccacgagatc taccgcaacc ttttcctaaa acgttacatg
     4501 tcgttccacc acgcatgaat ctctatatta agcgaaatat gcaggtaaac aatattttca
     4561 gaaggtatgt agcagacgaa gatttattga tttactcgat cgatgaatcg atcttaaaag
     4621 tgaccagatc aatgaatctt tttacgactg aaggaacaag aagtcaacgg agaaagaagc
     4681 tggctcaaat gatccaggag aatattaaag aagagctagg attgcttgct acagtaggtg
     4741 tcggagataa tcccttatta gctaaacttg ctttagacaa tgaagcaaaa cataatgatg
     4801 gattccttgc tgaatggacg tatcaaaatg ttcctgataa agtatggaat attcctgaaa
     4861 tgacagacct ttggggtatt ggatctcgaa tgaaaaaaag actcaaccaa attggtatca
     4921 tgagtattaa agacttagct aattggaatc catacactat taagagccat ttaggtgtga
     4981 ttggcttaca gctgtatttt catgcgaacg ggatcgatcg aacagatata gcgatcccac
     5041 cagaaccgac aaaggaaaaa tcatatggta attcacaagt gttaccacga gattatacca
     5101 ggagaaatga aatcgaactg gtggtaaaag aaatggcgga acaagtagct attcgtatca
     5161 ggcaacataa ctgtaaaaca ggttgtgtcc atttatacat tggcacgtcg atcttagaaa
     5221 cgagaccagg tttttcacac cagatgaaga tccctataac agataataca aaggaattgc
     5281 agaattactg tcttttcttg tttgataagt attatgaagg acaagaagta aggcacgttg
     5341 gaataaccta tagcaagctt gtgtacactg attcgcttca gctggattta tttagtgatc
     5401 cacaaaagca aattgacgaa gaaaatttag ataaaattat tgacaagatt cggcaaaaat
     5461 atggttttac gtcgattgta catgcttctt ctatgttaga aggcgctcgt agtattacta
     5521 gaagtacatt ggttggtggc catgcaggcg gcgcaggagg gcttaaaaat gattgattat
     5581 aatgatcgtg gacgtttaaa gtgggcaggt ttttatttat ctgatcatac tgaaaaaatt
     5641 gatgccgaca atgaacaacg agccaatcaa aatttggcaa aagaacaaat gacgactgaa
     5701 gaggttgcgg aagtattgaa cgctgcagtc ataaaaaata gatcagtatg tattcaaaaa
     5761 gaagaacgaa acgctgaagg atactatcca gaagatattg ttagcaagat cttaggtagt
     5821 gatgagctgg ggatctacgt tggaaaaaac aaagttcact acgacgaaat acgtcatatc
     5881 gaattttacc aggataataa atggggtcga ttagaatgaa cattatccag caatatgagc
     5941 taaagtatat aacctttgat cagctatcag aagagatttg gggttatggc cagcgtttga
     6001 taaatgaggt tggtgttgaa cgcttctctt tctatgtaga agctgcagca ggataccata
     6061 aatacgaata ctatattggc tattaaagtg caataaaatt atatttcttt tatgttttac
     6121 attttgtatc aattctgata aaattaccta taggtaatta aaaaatataa aaaaactagt
     6181 tacaacctta ggacgttgta actagcttga agcatactag agctaactaa aatagcacta
     6241 gaaagcaatg ttaaacccgt ccaacaaact tgatttaaca ttgagaccag cttattcagc
     6301 taaatatagt tgaataagct tcttttgttt taaaaaaaca aaaggtgtac tacgaataac
     6361 gcaagtaaaa gaaatactat tacaaccagt aggctacaag taagtattac taaacattgc
     6421 gtagttagaa cctttgctct attgtctaaa gccacaaaat tagagatcag ctttatgatg
     6481 ctaagagcga gctgcatcca tgcggacata ggttcttttg acactattaa ttctcctttt
     6541 taggataagg gaaattgcta gtagtttttt cattagcacc atcccccttc atttgattga
     6601 gtctagtgct gctgtgatag tttgctgttt aatatgctta cattcttatt ataacataaa
     6661 tgaaacaaac acatggttag aggtcgtatc aatcaaaata gtgtctcaca aacgatttat
     6721 tacttactcc tgctgactaa cggactgttc ttcagtaact gtaactatat gtttgttaaa
     6781 accaccatta cctgtagata aaaaccaata tatttactat actcactata ttaaatatat
     6841 ttagtatagt aaaaactaga aacaataaaa tttgtgaatg ttgtataccc ccaaacaata
     6901 cacatatttt gaaggattag gattaataaa gctaaaaata tagtaagtat actaaatata
     6961 tttactatat ctagtatact tactatattt taatcttttg atcgtctatt atatataatc
     7021 cgttcctcgt cgtttaaagt atcaacatag gcgtccattg caagtttgac tatttcatac
     7081 ataggaatat cttttgcata actaagatcc cgaatggttt ttagtacggg aggatctact
     7141 tgaactggtt ttctttcttt cgcagtgtac tttcgttccc ctaactcgac attaatatca
     7201 tctttaattg aaatatcttc agcaccaatt ttcttcttat ctttgttcgt tggtaataat
     7261 gccatttagt ttaatctcct ttccaagagg taggatatga ttggccattg agtcagcata
     7321 tttaggtgta taatcaaatc cttcaatatc accatgttgt tcatagtatt caattctttc
     7381 ttctaattct gtaaagatgt ctgcaaaaat agcccaccat cttcgatcta cataatcatt
     7441 taattggata ccattctcac catacatttc taaccgatcc gagcctttaa caatgctttt
     7501 aaaaatgttt gattcaccat acttatcaat tgttttttga tagtttgcgt gttgagagga
     7561 acgtcgcact gagaaaagaa caggcaaaat tccaatgatt tgaagatgag actcttcaaa
     7621 attcgtgaca atcggaacta aaactttatt aataaaatct tctgttcctt ccattgcgta
     7681 ccgttttaat tcttgcattg caataatata atctgcagca gttaagaatg cacgaaccac
     7741 attatctgtt gatggtgggc agtcgataaa gatataatcg aaattttttc tcaattcttc
     7801 taaaggttta atgaaggcca atgatctctt acgacgatca ggatattttt cggacaagta
     7861 ttcattaagt tcttcagatc caggagagcc agccataata tataaatgtg gacttacttg
     7921 agtaatacct gaaacccagt cccctttttt gattgctgca gacatggatc gatcaaagct
     7981 agtaatttga tacgttgatc caatgttgga agtagtattt ctttgttggt cataatcaat
     8041 tagcaacacg tctttttcaa aaagttgact agaaacaatc gctaataaat cattgtctgt
     8101 agtcttacca atgccgcctt ttctacctgt aagcattaca acttttccta gtttatctga
     8161 tgttttaaat gcagtccgta agttttccat agttccctcc aatttcctat actaaatata
     8221 tttagtatac ttcatatact taatatattc gatatatcta atatactcca aattgataag
     8281 aattcagact ttataaggaa tatagtggat atactaaata tatttgctat attcagtata
     8341 tcagatatat tcaaaatgtc aaaatatatt tgtgcgtttt gcgttgacaa tttagagtga
     8401 aaagaggtaa acttaattct atagaaacca cagataattc actatttaca aagtagcaaa
     8461 agcagtggta ctctttaaac agaaaaatta cataaaaaaa agagacggaa ataattgatt
     8521 ttagcagaat caattatttc tgaatcacat ggttaggaca tgtgttcacg ctcgtctcaa
     8581 gaatatgtaa gtagctacca actacttata ttaaaagttg atcggatacc ttttccgatt
     8641 gcctttattc taccaaaaaa aggcgagcaa ctcaacagaa gaacgtttat ttagtgtact
     8701 taaattaaat attcttaaac actgaagaga gaacacatac tatggctgag attaaagcta
     8761 aactgtgttc tctttttgtt tgagaaagga cgttacaatc gatgttgagc gaaatttatt
     8821 cagctattct taaaaacggc ttacgtacat acaaatatcg aaacagcacc atgaaacctg
     8881 caggtcatat tgattccagt aggagtggtg ctatctttgc gtaccgtagc aagcaattaa
     8941 tgaatgttgg gcgtggtatg gtcattactt cggaagaagc tattttagag aacgaaaaga
     9001 aactaacgca ttggacacct aatacctatc gctttggtac ttatgcggat gattatcgaa
     9061 cagtggttaa aggtcattct gagaaaaact taagtcaaat caacacattt gtagttgata
     9121 tcgattcaaa agagaaccat caaggcgaaa ttattttagc ctgtttagat caagtgggct
     9181 acatgccaac gctaatttta gaatcagatc atggttatca agtttatttt gtactaaaga
     9241 caccaggcta tgtcacaaag aaaagcaatt tcaaggtgat cgatgtagca aaaaaaattt
     9301 caaaaacaat tcgtctacag ttggctgaaa aattgtccgg agtggatcta ggatgtaacc
     9361 acttcggaat tgctcgattc ccacaagaaa ataatattgt ttttaaagaa atgaactatc
     9421 agtattcttt tgctgactgg attcaatggt cgatgaagct tgaatcagat caaaaaacaa
     9481 aagaagatcg agaacgaaaa ctaattgtct ttcctgagaa aaaagaaatt cgtcaagtag
     9541 atgaaccgtg gttcgatatg ttacttcata aagcaaacat cgtcggcgga gaaggacgtc
     9601 ttggtcgaaa taatgtgatt ttcacgcttt ctttagctta ttactcttca ggttatgcat
     9661 tggatacttg cgaatacaat atgttcacat tcaacgaacg gttacaggaa ccgctatctg
     9721 aagcagaatt agtaaagatt gtacgtagtg cttattctgg taattatcaa gcagcaagca
     9781 gagaattcat tattgaattg tgccagcaat gggtttcacc aaccttaaaa aataatgatc
     9841 tgttcattca acgacgtggc tggtggaaat ttaaaaagcc acgagatcaa agaaaatata
     9901 gtcataaaaa tgaatggaaa gaagatctat tggcttatct aagcgaaaaa agtcttgcta
     9961 gtgagcctta tttgaacctg tcaaaaaaag aattggcttc acaactaacg atgcccaagc
    10021 gaacacttga taagatctta aatgagttac gagaggagaa taaagtcttc tacaaggtaa
    10081 cacgaggaag aaatggcgga atgatcattg cttcagtcaa aacgttgttc gcgagggtta
    10141 ttcaactaag taaagaaaag agactagatt attcaacaag gatcacagag gtatttggac
    10201 tcaacgagag cttagtagaa gagacattta gacaattatc aacccccttg aaccctctta
    10261 aagaagtgaa tctttttgaa gtagatacag gttgaaaaat aaaaggtgca aattgcctta
    10321 tacaatatat ctatgatact tctgtaggtg ttgctccctt tgcctagcta cgtgtaggtg
    10381 gtatacttat aactgcgtat gcagtcttat gtatcatgga ggaagttgta tgtcgttaaa
    10441 aaattatgtc tattcacatc ctgttaatgt gtttatcatt ggtcgtttgg gattatctgt
    10501 ggaagtgttc tgtgagcttt atggttttaa gcaggggact ttgtcttctt gggttaccag
    10561 ggaaaaaaca gttgcttctt tgccgattga gtttcttcat gggttgtcct tagcttctgg
    10621 ggagactatg gatcaggtat atgattgttt gtgtgtgtta gagcaggagt atattgagta
    10681 taaaatagct aacgagttaa gaaaaaggaa gaagtatatc cagtagtagt ttttatttac
    10741 ttaaaaaatt ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa
    10801 ggaaagaatt tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc
    10861 ttcctctgaa aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag
    10921 gaaaactttg tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag
    10981 tatttggaac ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt
    11041 ttacagtgct aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca
    11101 tgcaatttac caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa
    11161 ttgttgtaaa tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa
    11221 caaaaggagt gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt
    11281 tgtttgaagt gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac
    11341 gttcaattga aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca
    11401 atgttgattt tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc
    11461 atgaatctac tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa
    11521 tagatcgaag aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca
    11581 tcggaaaatt gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga
    11641 acagtagtga tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat
    11701 gattcctcta tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga
    11761 gcagttattc aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat
    11821 gaacattgaa taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat
    11881 tcgaaagcaa caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa
    11941 tgggaacaat tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag
    12001 agcataagga aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta
    12061 cgtttctcaa aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt
    12121 gaaggatagt aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc
    12181 tttttaaatt ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt
    12241 aagagaaatt gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc
    12301 aaataaggta gaataataga aaaagatagc aggaggaatg acgatgaatc
//
LOCUS       DAYLZO010000085        11874 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.086, whole genome shotgun sequence.
ACCESSION   DAYLZO010000085 DAYLZO010000000
VERSION     DAYLZO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.086"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1106
                     /gene="ligA"
                     /locus_tag="ACGVJG_002147"
     CDS             <1..1106
                     /gene="ligA"
                     /locus_tag="ACGVJG_002147"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFJ6558069.1"
                     /translation="AYKFPPEEVETLIENIEWTVGRTGVVTPTAIMTPVRVAGTTVSR
                     ASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVLDKRPKDSEEYQLPTHCPVCGS
                     ELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNIDGLGPRVLEQMYDKKLVADVA
                     DLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVERLIFGLGIRHVGAKAAKILAE
                     HFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNEEVHELMNELKQAGVNFEYKGL
                     RNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIENLGGKVTGSVSKKTDIVVAGED
                     AGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            1212..1517
                     /gene="gatC"
                     /locus_tag="ACGVJG_002148"
     CDS             1212..1517
                     /gene="gatC"
                     /locus_tag="ACGVJG_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFJ6558070.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            1517..2989
                     /gene="gatA"
                     /locus_tag="ACGVJG_002149"
     CDS             1517..2989
                     /gene="gatA"
                     /locus_tag="ACGVJG_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFJ6558071.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            2986..4416
                     /gene="gatB"
                     /locus_tag="ACGVJG_002150"
     CDS             2986..4416
                     /gene="gatB"
                     /locus_tag="ACGVJG_002150"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFJ6558072.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            4426..5481
                     /locus_tag="ACGVJG_002151"
     CDS             4426..5481
                     /locus_tag="ACGVJG_002151"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFJ6558073.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            5592..6965
                     /gene="rlmD"
                     /locus_tag="ACGVJG_002152"
     CDS             5592..6965
                     /gene="rlmD"
                     /locus_tag="ACGVJG_002152"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFJ6558074.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            6968..7096
                     /locus_tag="ACGVJG_002153"
     CDS             6968..7096
                     /locus_tag="ACGVJG_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFJ6558075.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            7520..7708
                     /locus_tag="ACGVJG_002154"
     CDS             7520..7708
                     /locus_tag="ACGVJG_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFJ6558076.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            7786..8799
                     /locus_tag="ACGVJG_002155"
     CDS             7786..8799
                     /locus_tag="ACGVJG_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFJ6558077.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(8866..9900)
                     /locus_tag="ACGVJG_002156"
     CDS             complement(8866..9900)
                     /locus_tag="ACGVJG_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6558078.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            10015..>11874
                     /locus_tag="ACGVJG_002157"
     CDS             10015..>11874
                     /locus_tag="ACGVJG_002157"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFJ6558079.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDL"
BASE COUNT         3975 a         2156 c         2555 g         3188 t
ORIGIN      
        1 cagcttacaa gtttccgcca gaagaagttg aaacgctaat cgaaaacatc gaatggacag
       61 tcggtcgaac aggggtagta acgccaacag ctatcatgac accagtccga gtagcaggaa
      121 cgacagtaag ccgtgccagc ctgcataacg gtgattatat taagttgaag gatatccgct
      181 tgaaagatac agttctgatt tataaagcgg gagacatcat cccagaagtt tcgcaagtcg
      241 tgttagataa acgacctaaa gacagcgaag aataccaatt gcctacacat tgtccagttt
      301 gtggaagtga acttgtccat ttggatgaag aagtagcctt acggtgcatc aatcctaaat
      361 gccctgctca aatgaaagaa ggtttgaacc actttgtttc aagaaatgcg atgaatatcg
      421 atgggttggg gccgcgcgta ctagaacaga tgtatgataa aaaactagta gcagatgttg
      481 ctgatcttta caaattgaca gaagaagagt tattgacgtt ggacaaaata aaagaaaaat
      541 cagcaaataa tattttgaca gcaatcgaca acagcaaaga taattcggtc gagcggttga
      601 ttttcgggtt aggaatccga catgtcggag cgaaagcagc aaaaatttta gcagaacatt
      661 tcggtgatct agaaacattg agtaaaagtg actatgagtc gattattgcg cttgatacga
      721 tcggtgatat cattgccgac agtgtagtca cttatttcag taacgaagaa gtccatgaat
      781 tgatgaatga attaaaacaa gcaggcgtca atttcgaata caaagggctt cgaaatgccc
      841 aattgcaaga agtcgagtct ccttttaaag aaaaaacagt agtccttact ggtaaactaa
      901 cacgatttac tcgtgaagaa gcaaaagaga cgatcgaaaa tcttggcggg aaagtgactg
      961 gtagcgtatc gaaaaaaacg gatatcgtgg tagcaggaga agatgctggc agcaagctga
     1021 ccaaagcaca agaattagga atcgaagttt ggacagaaga tcaaatggca gatgcattgg
     1081 ctaaaagtcg ctcagtagaa gaatgaaaaa tgagtttttt cagacaaatt tcaataaaca
     1141 atttcagaca cggcactctt gtgttaaaat agagagcaaa gtttttaact cgtttagaaa
     1201 gaaggggtag tatggcgatt agtgaagaac aagtgaaaca cgttgccaaa ttagcaaaac
     1261 ttgcgttcgc agatgaagaa cttgcaagtt ttaccgacca attaggtaaa atcatcgata
     1321 tggttgaaat gctggaagaa gtagatacag aaggtgttcc atttacgtcg aatgtggttg
     1381 aaacagtaaa cgtgatgcga caagatcggg cgcaagctgg atggaaccgc gatgaattat
     1441 tgaaaaatgt gccggaaaca gaagacggct ttatcaaagt acccgcaatc atcgataatg
     1501 gggaggctgg cgcataatga cagaattaca caatcgttct ctagaagaac tgcatcatat
     1561 gcttgtatcg aaagaaatca ctgtacaaga tttgacaaaa gcaacatttg aacgaatcaa
     1621 agaaacagag cctgaggtcg aagcattcat tacattgaac gaagaaaaag cattggaaca
     1681 ggcaaaagct ttagatgaaa aagggattga tgaaagcaac gtccttgctg gtattccaat
     1741 tggtatcaag gataatatcg taacaaaaga tatcttgaca accgctgcat ctaagatcct
     1801 ttacaatttt gaaccaattt atgatgcaac ggtcatggat aaaatctatc aagcagatat
     1861 gatccctgtt ggtaagctaa acatggatga atttgctatg ggtggaagta cagaaacttc
     1921 ttatttcaag aaaacaaaaa atgcttggga cccaacaaaa gtacctggtg gctcttctgg
     1981 tggttctgca gcagcagttg cagctggaca aattccagtt tctttaggta gtgatactgg
     2041 gggaagtatc cgccaaccag cagcttttaa cggcatcgta gggatgaaac ctacttatgg
     2101 ccgtgtctca cgttttggtt tgattgcctt tgcttcatct cttgaccaaa tcggaccaat
     2161 gacccgtacg gtgaaagata atgcattagc attgacagct atcagcggtt atgacgaaaa
     2221 agacggtact tcttcaggcg tttcagttcc tgattttgca gagggactga caggcgacat
     2281 caaaggaatg aagattgctt tacctaaaga atacttaggc gaaggtgtgg cccctggcgt
     2341 aaaagaagct gtattgaaag cagcagaaac attccgttca ttaggtgctg ttgtggaaga
     2401 agtcagcttg cctcattcaa aatacggtgt ggctgtttat tatatcattg cctcatcaga
     2461 agcaagttcc aacttgcaac gattcgatgg tatccgctac ggttatcgct cagaaaatgt
     2521 gaaatcatta gatgatgtat atgtcaactc acgttcagaa ggattcggtg aagaagtaaa
     2581 acgccgtatc atgttaggta cattctcctt aagtgctggt tattatgacg cttacttcaa
     2641 aaaagcggga caagtccgta cattgatcaa acgagatttt gaaaaagtct ttgcagatta
     2701 tgacttgatc atcgggccat cttcaccaac agttgcattc ggaatcggtg agaacattaa
     2761 cgatccaatc acaatgtaca tgagtgacat cttaacgatc cctgtcaact tggctggact
     2821 tccaggtatg tctgtaccgg caggtttatc agaaggatta ccagttggat tgcagatcat
     2881 cggtaattat tttgatgaaa aaacgatgta tcaagcggca tatgcgtttg aacaagcaac
     2941 agaattccat aaacaacaac cagctatttt aggagggaaa gacgaatgaa ttttgaaaca
     3001 gtcattggac tagaagtcca cgtcgagtta aaaaccaact caaaaatttt ctctccagcg
     3061 ccagctcatt ttggtgcaga accaaacagc aatacaaacg tgatcgactg gggttatcca
     3121 ggcgtgctgc cagtaatgaa taaagctgcg ctggaattcg ggatgaaagc agctttggca
     3181 ttaaactgtg aaatctctaa agatactcat tttgaccgga aaaattactt ttacccagat
     3241 aatccaaaag catatcaaat ttcccaattt gatcaaccaa tcggtcatga cggatggatc
     3301 gaaatcgaag tagaaggtaa aaagaaaaaa atccggatcg aacgtgtcca tttagaagaa
     3361 gatgctggaa aaaacatgca tggtattggc ggttattctt acgttgattt gaaccgacaa
     3421 ggcacgcctt tgatcgaaat cgtttctgaa gctgatatgc gttcaccaga agaagcatac
     3481 gcttatctag aagcattgcg atcaattatc caatttacag aagtcagcga tgtgaaaatg
     3541 gaagaaggct ctatgcgctg tgatgccaat atttctcttc gcccttatgg acaagaagag
     3601 ttcggtacga aagcagaatt gaaaaacttg aactctttat ctttcgtgaa aaaaggttta
     3661 gcttttgaag aaaaacgtca agcaaaagtt cttctttcag gaggagaaat ccaacaagaa
     3721 acacgccgtt ttgacgaaac aacgaataaa acagttttga tgcgtgtcaa agaagggtct
     3781 agtgattatc gttacttccc agagcctgat atcccgaaat tcgtgatcga tgacgaatgg
     3841 attcaaaaag taaaagctag tttgcctgag atgccagctt ctcgtcgcga acgctatatc
     3901 cgtgagtttg gacttccaga gtatgacgca atggtattga cacttacaaa agaaatgtct
     3961 gatttttttg aagctgtttt ggaaaatggt gcagatgcca agcaagcatc taactggttg
     4021 atgggtgaag tttctgctta cttgaacagt gagaaactgg aattagctga gacaaaattg
     4081 acacctgaga acttagcagg aatgattcgt ttgatcgccg atggaacgat cagctcaaaa
     4141 atcgccaaaa aagtcttccg tgaattgatc acaaatggcg gagaagccca agaagtagtt
     4201 gaaaaaaatg gttgggtgca attatctgat ccgaacaaac tactgccaat cattaatgaa
     4261 attttggata acaaccaaca atctgtcgat gatttcaaaa atggaaaaga tcgtgcggtt
     4321 ggcttcctag tcggtcaaat catgaaagca actaaaggac aagcaaatcc tggtgtcgtg
     4381 aataagctac ttaaagatga attagcgaaa cgatagaggc tgaaaatgaa aaaagcacgc
     4441 gtaatttata accccacctc gggaaaagaa ttattgaaaa aaaatttggc ggatatccta
     4501 caggcattag aagaagcagg atttgaagcc agcgcctatg caactacacc agaaccagat
     4561 tcagccaaga atgaagcatg tagagtcgca gaactaggtt ttgatctagt agtggcagct
     4621 ggtggcgatg gcacaatcaa cgaagtggtt aatggaatcg ctccgcttga acaccgtcct
     4681 aaaatggcca tcatcccagc tggtacaacg aacgactatg ctcgagcatt aaaaattcct
     4741 agagataata ttaaagcagc agcagaagtg ataaaaaaac accaaaccgt aaaaatggac
     4801 atcggccttt ctgataacag ttacttcatc aatatcgctg caggtggcta tttgacagaa
     4861 ttgacttatg aagttccatc agaactaaag agtatttttg gttatcttgc ttatcttgcg
     4921 aaaggagcag agatgctccc tcgagtaaag ccaatcaaaa tgcgcttgaa gtatgatgaa
     4981 ggggaatata tcggaaatgc ttcgatgttt ttcctagggc tgacgaactc tgttggagga
     5041 ttcgaacaga tcgcgccaga tgccaaattg gatgatggga agttttcttt aatcattgtg
     5101 aagacagcaa atatctttga gatcttgcat attgcagcac tcatgctgaa tggcggtcga
     5161 catgtcgatg atccaagaat aatctatacc aaaacaagca gtttgttcgt tgaaacagaa
     5221 gatgatatca atcgaccagt aatgatcaac ttagatggtg aatacggcgg ggaagcaccg
     5281 atgcatttcc aaaatcttca tcaacacatt gagttctttg ccaataccga tcagattcct
     5341 gatgaagcaa tcacaggtac agatgaagaa gaattagagg ttgtcagcaa agaattcgta
     5401 aaagaagtcg aacgtttaac ggatgaagat atcgacggtg acggaaaaat agcagacgaa
     5461 acacctaatc aaaagaatta atcaaaagcc ggtgctgaac gtagtaaggt cagtgccggt
     5521 tttagttgtg taattagctg atttatagta gagttcagta ggattgaaaa ataggcttaa
     5581 aggagcaaaa tatgacagaa cagcaaatag taaagaaaaa cgaacgatat acagtcgaaa
     5641 tcgtcgactt gagttatgaa ggaatggggg tagcaaaaat cgatggttat ccattattta
     5701 tcgaaaatgc actccctgga gaagaagtag aaatactagt agtaaaagca ggcaataagt
     5761 ttggatatgg aaaagtcgaa acatttatta cacaatcacc tgatcgtcag cctgtagaaa
     5821 acaatgcatt acttcgcata ggtatcgcac ctttagccca tttgaagtat gaacaacagc
     5881 ttgtcttcaa acaaaaacaa gtagagcatg taatggcgaa gattgctaag atgccagaag
     5941 ttcctgtact tccaacggtc ggaatggcag aaccattcgg ctaccgtaat aaagcacaga
     6001 tccctgttcg ccgtatcgat ggacagttag ccaccggttt ttacaaaaag aatagccatg
     6061 atctagtccc tatcgaagat ttttatatcc aagatcctgt catcgatcaa gcaatcaaag
     6121 tagtcagaga tatcttgcaa cggttccaag tacgaggata taatgaagaa aaaaatgaag
     6181 gacagcttcg tcacatcatt atccgtcgtg gtcattattc ccatgaaatg atggtcgttt
     6241 tagttactcg taaagaaaaa ttctttaaat ccaaggaaat tgctgaagct attcatgaag
     6301 aattgccaga agtggtttcg gtcatccaaa atatcaatga agaaaaaaca aatgtgattt
     6361 taggagacaa agaaaaagtt ttatatggca gagactatat cgaagatcag ctattaggaa
     6421 aaacctatcg aatctcagca aagtcatttt atcaagtaaa tactcaacaa gcagagtatc
     6481 tgtaccaaac agccatcgag tttgccgagt tagataaaaa agacattgtg atcgacgctt
     6541 attcaggaat aggtacgatt ggcttgtctt tagcagacag agtggataag gtgtatggga
     6601 tggaagttat tccagaagca attgaagatg cacagttcaa tgctttaaat aataaaatcg
     6661 aaaatgttca ttatgaagtc ggaaaagcag aaaaagttat gaaaaaatgg caagaaaaag
     6721 ggatcaaacc atctgtgatc gtagttgacc ctccacgaaa aggattagat catcatttta
     6781 ttgaatcagc aatcgaaatg tcaccagaac gaatcgtcta tatctcatgt aatccggcaa
     6841 cttttgcaag agatgctaag ttatttgcag aagcaggtta tgccgtgaag aaagttcagc
     6901 ctgtagatct tttcccgcaa acccatcata tcgaattagt cgcttcattc gttaaaggag
     6961 aataaaaatg gaccaaaaag aaaaaatgaa agaattgatc aaaaagaaac aaggtggagg
     7021 ccgatccaaa caaatggaaa cacctaagaa tgatcgtaaa aacatgagaa aaggccctaa
     7081 aatctttaat aaatagacgt gctaaagaaa gactaagaag tgttaacttt tgttattgtt
     7141 tcttagtctt tttagtggct ctttgtcaat aaggactgat gggttgtaca aaatcaaatc
     7201 tgggtcagaa tgaacgtcat tctgtctcag atttttcgtt atttcaattt gattaactga
     7261 ttgatcaaaa agatttttat tttcatattc tcaaatgatt caacactaaa tatacatgtt
     7321 tgacgcggaa attattattc gattttggcg gcatcagggg ggatgacgac gtagcagacc
     7381 cattatttta ctcaagaaaa tgtccgtgcc tattatttag aatacgatag tgaacgtgct
     7441 gggagttttg ttcttttaga gaaagtctct gaagataaat tagttgtctt agattttgac
     7501 tgtaacgaaa aatggcgaaa tggaagagca ccagcaagtg attgaccgga tctacgaagc
     7561 gacacgatat ctgcctttgg atcgtttgtg cttaagtcct caatgtggat ttgcttcaac
     7621 agaagaagaa aatgttttgt ttgaagaaca atggaaaaaa tttcttttgt caaaagcatt
     7681 gcagaagaag tttggggaaa atcattaaat catctaggag agcttgatag gaaataacac
     7741 tatggtataa tcagaatgct taatactaac tactgaggga gccttatgaa aaagaaaatt
     7801 cttaaattta tcggatggtt cttcggaatc attcttggga tcgcgtttgc aatctttctt
     7861 gccttccagc tttcccccag acctggagca ttcttgatca accatatgtt ttcaaatact
     7921 gttcaaatca ctgataaaaa aacgttcgat caagcagaaa aaaacgtggc gaaaaaaaca
     7981 gatttgacgt atgagtccaa gtataaagag aatacttatg acatctacta tccaaaaaac
     8041 agcaaggggc ctgttcctgt tctattttgg gtacatggtg gaggattcgt cggcggagat
     8101 aaatcgggtg tcaaagaatt tgcgacaaaa ttagtttcag acgccaacat cgcagtggtt
     8161 gcgatgaatt atgaattagc gcctgattca gaatacccta atcaagttct tcaagtgaat
     8221 gaactgatca aagatttatt agctgaagaa aaagacgaca aattgctaga catggaacaa
     8281 ttattttttg gtggagatag tgcaggttca caaatcgcat tgcaatatgc agctatacaa
     8341 acgaatgatg cgtatgctaa acaaatgaaa atcaaacagc tacttccaaa agatagtctg
     8401 aaaggaacca tttcttattg cggacctgtt gatttgaagc taacagcaca gcagcattcg
     8461 gatgatcgat ttatgaagtt tttcgtcaaa accgttgcgt ggtcgttgat tggaacaaaa
     8521 gattggaaaa cgagtgagca attgaaagaa gctagtgtgg ctgaccatgt ttctaaggaa
     8581 tttccgccaa cgtatataac tgacggaaat gcatactcat tccaggagca aggtcaagca
     8641 ttagaaaatc ggttgacaga attaaaggta cccgtgcaat ccttgtttta tacagataca
     8701 aaaaaagaga ttacccatga ataccaattt gattacacgc taaaagaatc gcaaaattgt
     8761 taccagcaga cattggattt tgtgaataag tataagtaag aggttgtgaa aaagccgttt
     8821 cgttttgagt atgaagaaac gaagcggctt ttcacaacct ctctttcaat acctttttgt
     8881 tcttgggata aaactttccc taacaatcaa tcgtgcagga taagtcaaaa tgattggttc
     8941 gtcccgtata ccgtcaattc gctccaatgc cattcgaaca gctgcttgcc caattgattt
     9001 cgcattgatt tgaacactgg tcaagccagg ggagaggttt gcgattgtct cgatattatc
     9061 aaagcttgct acaacaatct cttctccaat ttggatattt ttttgtttca atgcccgata
     9121 gacaccaatt gccattggat cgctcccgac aagaatggca gtaggaagag gggattcatt
     9181 ttctaataga ttagtcgcaa gtacctcacc ttgtttttct gtccagccac ctagatgata
     9241 agacatatat ttttctaagt gatgatcgat cataaaatgc tgataggcac gcatcctctt
     9301 ttcatttata gaaatctgct tcccaccgaa ttcatccata tcgactttat atcccccgat
     9361 ataggctatt cttttatgac cattttctaa gaacaattcc agtacggttt tagtcatccg
     9421 ttcaagatct gcatacacca tatcgacctc ttgctggatg tcagggttat ccacgacgac
     9481 tatttgcatg ttttgtttta acaaggcggt aatcgattct ttcgtaacag tcccaacaac
     9541 gatcaccgca cctaactgat ctagatcttc ccattgttta ggattatcag atagattata
     9601 gattcgattc atccctaaat gcagacgtcg agcttcttct tccatcccta ctcgcaaatg
     9661 tttgtaatag atatcctgtt cttcgctcgc tttgccgatc gtagagatca caccaatcga
     9721 aggcattctc tttttgatat atttacgagt actgatgtca taatccaacg cagcagctgc
     9781 ctctactatc cgttgccgag tagtctgagc cacatggatc gtctgatcgt tattcaacac
     9841 acgggaaact gtagcaggag agacatttgc ctttcgtgcg acatctctaa ttccaaccat
     9901 ttttacacac tcctcaaaaa aataaaaaat ttttattgac aaaattgttt agtaaacagt
     9961 atacaggaat tataaaaaaa agtaaacggt ttccgttttg ttgggagtga aatgatggat
    10021 attacgcata gaaaaacaga taaaaatttc atcttatttg atgaaaagag ccgagtcttc
    10081 catttacgaa atacatttct ttcttatctg ataaagatag aagaaagcaa tgtattggcg
    10141 catatttatt ttggcaaacc agtcaagcag tacaaagaca ataaaaatta tccccgaaga
    10201 gatcgtggat tttcaggaaa tgttccctta aatcctgacc ggtctctgtc gaaagataca
    10261 ctaccgcaag aatactcttc tcatggcagc atggactttc gaacgcccgc atcgatcatt
    10321 cagcgcaaga atggatcaga cttactggat ctgagatatg acagccatta tattacagat
    10381 gggaagccag agatcgaagg cttgcctcaa acttatgtga tggacaagtc agaggcacaa
    10441 acgttggtga tttcacttaa ggatagagaa acagcgattt atttcgattt gttctatacc
    10501 atatttactg atagagcagt gatcacacga tctgtaaaga taagaaatga aaccggagaa
    10561 acaatcaaac tagaaaaagc ggctagtttc cagctagact ttgcccatac taggcgattt
    10621 gatgaagtca ttgctttgcc aggagcacat gtcaatgaaa gacagatttc acggcaatct
    10681 gttttatctg gaacaaaagt atttgaaagc cgtcgaggaa cgagcagtca ccatatgaat
    10741 aactttattg ctttagttca tcatcatacg acagaaaata ctggagaagc gatcggactt
    10801 caatttgttt attccggcaa ccattctttc gaacttgaaa aagatcagat caatcaattg
    10861 agagtcgtcg gtgggatcaa cagccatcgt ttttcgtggg aattgaatgc aggccagtct
    10921 tttcaaacac ctgaaatgat cctttcttac tcaagtcagg gattgaacaa aatgagtcag
    10981 atccaccatg agttgctacg tgaaagaatc gcaagaggtc gtcatcaatt tgctgaacgg
    11041 ccgattctgg tcaataactg ggaagccact tactttgact tcaatagtga aaaaatcaag
    11101 gcgatcattg atgaagcaaa ggagctaggc atcgaaatgt tcgtattaga tgatggctgg
    11161 tttggaaaaa gagatgcgga caattcttca ttaggagact ggttcgagta tgaaggcaag
    11221 ttgacaaatg gattacgtga aatagcagat tatgctcata gcaaaggatt gaaatttggt
    11281 ttatggtttg aacctgaaat gatctcggtt gattctgaac tgtatcgaac acatcctgat
    11341 tttcttatgc aggagccagg tagaatgcct tctgcttcca gatcacaaca tgtactggat
    11401 tttacaaggt tagatgttcg gcagacgatt gagaaacaaa tgagaaagat tttagatacg
    11461 attcctcttg actacatcaa atgggatatg aaccgttcgc tttcagatgt ctattcgatt
    11521 acccttgacc cacagagaca aggagaagta gcgcatcgtt atatgctcgg gctttatgaa
    11581 ctacttgaac atttgacaac tgattatccg gaaatcttat gggaaggctg ttctggtgga
    11641 ggtggacggt ttgatgccgg gtttatttat tatatgccgc agtcatggac aagtgataac
    11701 acggatgcag tggagcgtat gaaaatccaa tatggaacaa gccttgctta tccgatttcg
    11761 tccatcacag ctcatgtatc agctgtacca aatcatcaga ccgacagatc tacttcgttg
    11821 aaaacaagag gagaaacagc tatgagcgct gtttttggtt atgaactgga tttg
//
LOCUS       DAYLZO010000086        11766 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.087, whole genome shotgun sequence.
ACCESSION   DAYLZO010000086 DAYLZO010000000
VERSION     DAYLZO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.087"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..556
                     /locus_tag="ACGVJG_002158"
     CDS             <1..556
                     /locus_tag="ACGVJG_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HFJ6558080.1"
                     /translation="GGYQTGDTAYLYSIVEMVPSEDPDYVLYLTMKHPKTYDRMALAK
                     IANPLMKRAMDFKETEEDSDTETKTEKVSVADYRNLEADVAAADAQKSGLQPVVIGNG
                     KKVQKQSTANGDQLISGEKLILYTGGDKLMPDVTGWSKADIMKLGKILGIEVSFDGDG
                     YCVKQELAPYEKITEDKLSFTLEE"
     gene            582..1544
                     /gene="mraY"
                     /locus_tag="ACGVJG_002159"
     CDS             582..1544
                     /gene="mraY"
                     /locus_tag="ACGVJG_002159"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFJ6558081.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            1558..2919
                     /gene="murD"
                     /locus_tag="ACGVJG_002160"
     CDS             1558..2919
                     /gene="murD"
                     /locus_tag="ACGVJG_002160"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFJ6558082.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            2945..4033
                     /gene="murG"
                     /locus_tag="ACGVJG_002161"
     CDS             2945..4033
                     /gene="murG"
                     /locus_tag="ACGVJG_002161"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFJ6558083.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            4048..5268
                     /locus_tag="ACGVJG_002162"
     CDS             4048..5268
                     /locus_tag="ACGVJG_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFJ6558084.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            5424..6749
                     /gene="ftsA"
                     /locus_tag="ACGVJG_002163"
     CDS             5424..6749
                     /gene="ftsA"
                     /locus_tag="ACGVJG_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFJ6558085.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            6772..8013
                     /gene="ftsZ"
                     /locus_tag="ACGVJG_002164"
     CDS             6772..8013
                     /gene="ftsZ"
                     /locus_tag="ACGVJG_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFJ6558086.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            8030..8707
                     /locus_tag="ACGVJG_002165"
     CDS             8030..8707
                     /locus_tag="ACGVJG_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFJ6558087.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            8721..9320
                     /locus_tag="ACGVJG_002166"
     CDS             8721..9320
                     /locus_tag="ACGVJG_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFJ6558088.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            9401..9622
                     /locus_tag="ACGVJG_002167"
     CDS             9401..9622
                     /locus_tag="ACGVJG_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFJ6558089.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            9655..10452
                     /locus_tag="ACGVJG_002168"
     CDS             9655..10452
                     /locus_tag="ACGVJG_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFJ6558090.1"
                     /translation="MGGENFDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFL
                     DPRQSYILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYP
                     SKFASLSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVA
                     VRLEPIKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNW
                     TENTRPDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            10473..11180
                     /locus_tag="ACGVJG_002169"
     CDS             10473..11180
                     /locus_tag="ACGVJG_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFJ6558091.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            11442..>11766
                     /locus_tag="ACGVJG_002170"
     CDS             11442..>11766
                     /locus_tag="ACGVJG_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HFJ6558092.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANK"
BASE COUNT         4132 a         1988 c         2568 g         3078 t
ORIGIN      
        1 agggggatat caaacaggag atacagccta cctgtattcg attgtggaga tggttccttc
       61 tgaagatcca gactatgtct tgtatctaac aatgaaacac cctaagacat atgatcgaat
      121 ggcattggct aaaatcgcaa atccattgat gaaacgggca atggatttta aagaaactga
      181 agaagattca gatacagaaa caaaaacaga aaaagtatcc gtagccgatt atcgtaatct
      241 tgaagcggac gttgcagcag ctgatgctca aaaaagtgga ctgcaacctg tagtgatcgg
      301 taatggtaaa aaagtacaaa aacaatctac tgccaacggt gatcagttga tctctggaga
      361 aaaactgatc ctatatactg gtggtgataa gctgatgcct gacgtaacag gatggtcaaa
      421 agcagatatc atgaagctgg ggaaaatcct cggtattgaa gttagttttg atggagatgg
      481 ttattgtgtc aaacaagaac ttgctccata tgaaaaaatc actgaagaca aacttagttt
      541 tactttagaa gaataagaag agaaatagga gagaaaagag catggaatgg acacaagcat
      601 tgattcccat tgtcagcagt tgtgcaatga caattgccgc aatgccgcta tttattggtt
      661 attttcaaat gaaaaagcaa ggtcaggcga ttcgtgagga gggaccaaag tggcacaata
      721 gcaaagccgg aactcctaca atgggcggct tagtgtttct gatcggctct attttgacag
      781 gaatttgggt tggagcttgg caaaaacagc taactcctac tcttttcatc ttgctttttg
      841 tattagcgtt atacggagta atcggctttc ttgatgattt tataaaaata ttcaagaaaa
      901 gaaacatggg actgaactcg aagcagaaat tgattggaca gattctaggg ggaatcatct
      961 tttatcttgt ttaccgttct gaaggttatc caggtgtatt gaatttcttc ggactagatc
     1021 tacctttagg aatcatttat ggtattttcg ctctcttctg gcttgttgga ttttcaaatg
     1081 cagtaaatct gacagatggc atcgatggat tagttgctgg cttaggaagt atctcttttg
     1141 cagcttacgg tctaattgct tggcatcagc atcagtatga tgtattaatc atttgcttaa
     1201 gtgttttagg aggtttactg ggtttcttcg tctacaacag aaaacctgcc aagattttta
     1261 tgggagatgt cggttcatta gcgttgggcg gacttttagc ggcaatttct ataatgctaa
     1321 accaagaatg gacattgctt ttgattggtt tgatctatgt tatggaaaca gcaagtgtga
     1381 tgcttcaagt tacttccttt aagcttacag gaaaacgaat ttttaaaatg tctccaattc
     1441 atcatcactt tgaaatgtgc ggatggtcgg agtggaagat tgatacagtt ttttggttga
     1501 tcagcattgt gacttcgttg atcacattat ggatcgtttg gtaggagatg acgggaaatg
     1561 aaaaaaatat caacatatga aaataagaaa gtgcttgtct taggactcgc aaaaagcgga
     1621 gtaagtgcgg ccaaattatt acatgaattg ggcgcacttg tcaccgttaa tgatggaaaa
     1681 ccttttgacg aaaatccgga agcacaagag ctgctttctt tagggataaa agtaataaca
     1741 ggtagtcatc caatcgaatt gttggatgaa gaattttcat tgatggtcaa aaatccaggg
     1801 attccctaca gtcatccttt agtagcaaaa gcccaagaaa tgggtattcc ggtaattacg
     1861 gaagttgagc tagcttacga agtagcggaa tgcccaatta ttgggattac aggaacgaac
     1921 ggaaaaacaa cgacaacaac gatgacagga ctactactga atgcaggagc agatcaagga
     1981 attgcacgtt tagcgggtaa tattgggtat ccagcaagtg gtgtagcgca agaagcaaaa
     2041 tcagaagata aaattgtgat ggaattgtct agcttccaac tcatgggaat cacagatttc
     2101 cgccctcata tagcggtgat taccaatatc tatgaagcac atattgatta tcatggtaca
     2161 agaaaagaat atgtcaaagc taaatggaac cttcaaaaaa acatgacaga aaaagattat
     2221 ctgatcctta actggaatca gtcagaacta caagaattag cgcaaagaac aaaagcacgc
     2281 gtactgcctt tctcgacaaa agaagtgttg gaagacggtg tgtatgctga cgattactca
     2341 atatactata aaaaagaaaa aataatggaa atatctgaac ttggcgtacc aggtaagcat
     2401 aatgtagaaa atgcactagc tgctatttct gtagcaaaat tgtatggtat atcaaatgaa
     2461 gctatacgag aaacgttgca ttttttccat ggcgtaccac atcgtacgca atatgtagga
     2521 gaaatccaag gcagaaaatt ttataatgat tcaaaagcaa ccaatatttt agcgactaaa
     2581 atggctctaa gtggatttga aacgtctaag gtcgtgttat tggctggcgg attagacaga
     2641 ggaaatacat ttgatgaatt aattccttca ttaaaaggaa tcaaagcaat ggtcgtattt
     2701 ggccaaacaa aagaaaagtt gatggatgct ggaaaaaaag caggcatcga gacaatagta
     2761 acagccgatt ctgtagaaca agcagttcct ttagcattag aaaacagtac agatggtgat
     2821 gtagttttat tatcaccagc gaatgccagc tgggatcaat acccaaattt tgaaactaga
     2881 ggaaacagat tcatggaagc tgtgaaccga ttaaaataga aaaagtagaa gcgagcgata
     2941 aaaaatgaaa attttggtta caggcggagg aacaggggga catatttatc cagctcttgc
     3001 ctttgtcaat tatgtaaaga ctcaagaacc gaatgctgaa ttcatgtatg tgggagcaaa
     3061 acgaggtcta gaaaacaaaa tcgtccctga tacaggaatg cctttccata ccttggagat
     3121 tcaaggattt aaacgaaaaa tttccatgca taacatcaag acggtccgat tgtttcttaa
     3181 gagtatccgt caagcaaaaa aaatcctcag tgattttcaa ccagatatcg ttatcggtac
     3241 tggaggatat gtttccggag cggtagtgta cgctgcagcc aaaatgggta ttccaacagt
     3301 tatccatgaa caaaacagtg taccaggtgt taccaataaa tttttaagcc gatatgtgga
     3361 tcggatcgct ctttcattta aagatgcggc ttcctttttt cctgaaaaca aagcagttct
     3421 tgtaggtaat cctcgagcac aagaagtagc agatactaaa aaatcagata ttttgaaaac
     3481 atttgatttg gatccagaaa agaaaacagt cttgattttt ggcggtagtc aaggagcact
     3541 taaaatcaat caagcagtat cagttttttt aagtgaatat attccttctt cttaccaagt
     3601 tttgtatgct tcaggagata gatattatga agagatccta aataaaataa atcctgaaat
     3661 acaaaatatt agtgtccagc cttatatcaa aaatatggca gaggtcatgg cttccagtga
     3721 tctgctggtg gggcgtgctg gtgcaacttc gatcgcagaa ttcacagctc tggggttacc
     3781 ggcagtgttg gttcccagcc catatgttac gaatgatcat cagactaaaa atgcaatgag
     3841 tcttgttcac gcaggagcag caaaaatgat tgcagataat gaactaacag gtgaatcact
     3901 aagtcagaca gtcaacgaaa tcatgggtga tgaagaattg caaaagcaaa tgtgccgagc
     3961 ttctaaagaa caaggaatcc ctgatgcttc aaaacgtctt tatgacttag taaaacaaat
     4021 aatcaaaaaa taaaggcggt ggtcttagtg agtaagaaaa aagaaccaaa aaaattgtct
     4081 catccgtctt ctgatgaaaa caatttgact ccttggcaaa aggctaaccg aaaatatctg
     4141 gcagaacaag agatgaaaaa ccaagagcca aaaaaagaag acataacatt agatacaccc
     4201 ctttcagatt ctgaagaaga aaaaacggtt caagaggaac agcaagaaga aagccagtcc
     4261 gagaaaaaaa tcgaattttt tgaagagatc aagcaaaaga aaaacggtgg accatataac
     4321 ggttcttttt tgaaccggct gcccaattta aaaagtcaac ggcacaaagt actatttcgt
     4381 cggttagctt tgatcatcac tgtattgggt ataccgctga tttttttgat ctattatgtt
     4441 tctccttata gcaaattaca agcagttgca gtatcaggca ataagacagt caacagccaa
     4501 gaaatcatct ctgatacaaa attgtcatta ggagaaaatg tttggggaca gtattttcat
     4561 cgatctactt atatcgatcg ccttaaaaaa gcacagccaa gaatcgaaac agcaaatata
     4621 catttcaaag ggatgaatga attcgatttg gatgtaacag aatataaaga gattgctttg
     4681 atcgcaaaaa acaatcaata ctacccagtg attgaaaatg gaacagtatt agatgaaaaa
     4741 gtagcaaatc ctacaaaaaa tctccctatc ttggaagagt tcaaagactc tgcaaaaatc
     4801 aaagaattaa caaagcaata caatcagtta tcatcagaat tacaaaaagc aatttctgaa
     4861 atcaagtaca cgccaagagc aagcaataaa aatctgatcc aattgaatat gaatgacggc
     4921 aatcaagtta ttgtaaatat caataatctt gcgaaccaaa tgaaatatta ttcgcaagta
     4981 gccaaagata tggatgaaaa aggtgtgatc gacatggaag ttggtatctt ctcgcatcct
     5041 tacggtagca ctcaagaaac ggaagaatcg ggccaatcgg aagaaacaac tgaaagtgaa
     5101 gaaaatgcgg atcaaacaaa cgaatcaagt gaagaggcag aaacgcaaaa agcacaaaca
     5161 cctggatcgt ctagcacaaa cggatcatct gattcaaatg attcaagtga aacaagtcaa
     5221 atagaagata atccaagtac aactccgtca agtttaaatg aaaattgata aaacaacttt
     5281 aaaaagtaag aatgtacttt ctaaaaacca gtaatttatg gtagaattgg aagcagtgaa
     5341 tatatctatt aataagtata ttttaaaacg gacgctagtt gttcgcttgt atatggaata
     5401 gtaagtagga gggaatccca ttcatggcaa aaacaggaat gtatgtaggc cttgatattg
     5461 gaacgacatc tgttaaagtt gtcgtggctg aatatatcga cagccaaatg aatattattg
     5521 gagtaggaaa cgcaaaatca gaagggatta accgaggcat tatcgttgat atcgataaaa
     5581 cggtccaagc gatacaacga gcagtacgac aagcagaaga aaaagcaggt atccagatca
     5641 aaggcgtaag tgtcggtttg ccagcaaaca tgcttgaagt agaaaattgc cagggaatga
     5701 ttgcagttaa cggagattca aaggaaatca ccgatgaaga tgtcagaaat gtggcttcag
     5761 ctgcccttgt tcgttcaata cctcctgaac gtcaaatcgt gtcaatattg ccacaagatt
     5821 ttactgtcga tgggttcgag ggaatcaaag atcctcgtgg aatgatcggc gtaagattag
     5881 aaatgtacgg tctgttgttt acaggaccta aaacgatcat ccataacata cgtaaatgcg
     5941 tggaaaatgc cggattaatc gtcaacgaaa tggtgattac tcctttagcg ctgactgaat
     6001 caattctttc tgacggagaa aaagattttg gtacgatcgt cattgatatg ggtggcggcc
     6061 aaacaacaac ggctgttatg catgataagc aattgaagtt taccaatctt gaccaagaag
     6121 gtggcgaatt tgtcactaaa gatatctcta ttgtattaaa tacgtcattt aacaatgcag
     6181 aagcgttgaa aataaactac ggggatgcat acccagaacg gacatcacca gatgaagaat
     6241 ttccagtaga tgtgattggt cagtcagaac cagtgaaagt cgatgaacgc tacttgtcag
     6301 aaattatttc agctcgaatg gaacagattt tcaacaaagc aaaagatgca ttggaccaaa
     6361 tcgaagcatt agagttgcca ggtggcgtcg tattgacagg cggagctgct agtcttcctg
     6421 gtgttgtgga cttggcacaa gaaatcttcg gcgtaaatgt aaaattgtat gttccaaatc
     6481 acatggggct gagaaacccg gtatttacaa atgtaataag tatcgtggat tattcagcaa
     6541 acttgagtga agtgtatcaa ttggcaaaaa ttgctgttac gggtgaaacc tcagcagctc
     6601 gtcaaatggt agttgaacaa gaaacaacaa acacatatga gagctatgaa gcacctgaag
     6661 aaacaatcta cgatgagccg gaaccaaaag agtccggaga aaatgtaaaa aataaaatca
     6721 agggtttctt tacaaatatt tttgattaat tcatcctggg aggaataaga tatggaattc
     6781 tcaattgata ataatatcaa tgacggcgca gtaatcaaag tcatcggagt cggtggtggc
     6841 ggtggaaatg ctgtaaaccg aatgattgaa gaaaacgtta aaggtgttga attcattaca
     6901 gccaacacag acgtgcaagc attaaaaaat tcaaaagctg aaacagttat ccaattaggc
     6961 cccaaatata ctcgtggttt aggtgctggt tctcagccag aagttggaca aaaagcggca
     7021 gaagaaagtg aacaatcctt acgcgaagct ttagacggtg cagatatgat tttcatcact
     7081 gcaggaatgg gtggaggaac cggtacaggt gctgcaccaa tcgttgcagg tatcgcgaaa
     7141 gaattgggcg cattgactgt tggtgtcgtg acacgtccat tcacttttga aggaccgaaa
     7201 cgtggccgtt ttgctgcaga aggtatcgct cgtttgaaag aaaatgtgga tacattatta
     7261 attatctcaa ataaccgttt attagaagtt gtagataaaa agacaccgat gcttgaagct
     7321 ttccgtgaag cagataatgt tttacgacaa ggtgtacaag gtatctctga tttgatcaca
     7381 gcgccaggtt acgttaacct tgactttgct gacgtgaaaa cagtgatgga aaaccaaggt
     7441 acagcattga tggggatcgg tgtggctagt ggagaagatc gcgtagtaga agcaacgaaa
     7501 aaagcaatct cctctccatt gcttgaaaca tctattgatg gagctgaaca agtacttctt
     7561 aacatcacag gcggtttgga tatgacattg ttcgaagcac aagatgcttc agatatcgta
     7621 gcaaatgcag caactggcga tgtaaatatc attctaggta catctatcaa tgaagaaatg
     7681 ggcgatgaaa tccgtgtgac ggtcatcgca acaggtatcg atgaatcaaa aaaggaacgc
     7741 aagtctagcc gaccagctag acaagcacaa atgcaatcac ctgctcaaaa gactgtatta
     7801 gatatggatc aagctaaacc aacttcttca gaagaagaaa acagttttgg cgattgggat
     7861 atcagacgtg aacaaaatgt tcgtcctaga gttgatgatt ccaattttga caatatcgaa
     7921 aaaaaagaat ttgatacatt caaccgcgaa gaaacaaaat ctcatggcga tgatgaatta
     7981 aatacgccac cgtttttccg tcgtaaaaaa taaaggagag gaagagcaca tgattgctga
     8041 caacttgcag aaaatcaagc aagaaattca ggattcgtgt gctcttgttc atcgtgatcc
     8101 gaaagaagtt acattagtag ctgtaacaaa atctgttgaa tccgctcaag ctgaagaatt
     8161 ggttagagct ggcgttatga attgtgcaga aaaccgagta gataaattat tagaaaagaa
     8221 acaagattta tttgattttc ctgaaataaa gtggcattta attggtaatt tacagcgcag
     8281 aaaggtaaaa ttgattgtaa atgaaattga ttactttcat gcgttggaca gtttaagatt
     8341 agcagaagaa atccagaagc ggttagagaa agaattatcc tgttttattg aagtaaatgt
     8401 ttctggtgag gaatcaaaac atggaatttc accagatgag cttttaccgt ttgtggaatc
     8461 tttagctgcc tgcacaaaaa tcaaggttgt tgggttgatg actatggcgc ctaaagaagc
     8521 ttctgatgag caaatccata aagtgtttgg tacgctgaaa gaattgcgtg atcttgttca
     8581 agcaaaaggt tatgcccatg caccatgtag tgaattaagt atggggatga gtcaagactt
     8641 tcaaattgcg attgaagaag gtgcaacatt tatccgtgta ggaacagcgc tattcaaaga
     8701 cgaataaagg aggaacaaaa atgccgcttt taaacagagg agcaattgct aattttttcg
     8761 gattagctga tgaggaagag tacgaatacc aagattatcc tgaaacaaca aaaaaggctg
     8821 tacagccaac taaaaaaaat acaacacaga atcaatcaac tcattctgta tctgctgcga
     8881 atactcatga aaaacaaaaa acggcgactt cacgtcctgt aacgcatgaa agtattcagc
     8941 cggctgaaaa gaaaaaagta attgaactac atcaatcacc tacaaaaact gtagcgaaag
     9001 aaagaacagc ttcaagaacc tctacacaac ctaaagcaaa tcttgctgga aaaatcactg
     9061 taatggaacc aagagcttat tctgaagcta tggcgattgc taaacgaatc attgctggag
     9121 aagcaacctt agtgaatttt catttagtag aagaaaaaca agcgagaaga atcgttgatt
     9181 ttctaacagg aacagtttat gcactagatg gagatatcaa acgtgttgga gatgagattt
     9241 ttctttgtac accgactggg atggagattg acagttcaac cgctcaatct tttgcagatg
     9301 cgaatttgtt tgatctgtaa gaggaggaac tacgatttat actattgcac tattacttca
     9361 tttacttcaa cgggcagcct atttctatag cattatcttg atggtatatg cattgctctc
     9421 ttggtttcca ggcgggtatc aatcagggct aggacgatta ttagctaaga tctgcgaacc
     9481 gtatatcagt ttgttcgatc gtctgccttt gaggattgga cccttggatt tcacaatttt
     9541 aggggcaatc ctgatcttga acttagccgt acaaggttta gcacagatcg ttatatggat
     9601 aactgtccat ttagtttatt gattgaagca acaaaatctt tattctcaga atagatagga
     9661 ggggaaaatt ttgatgcaaa tgtataccag cactttcggg cagatgaacg tacatttatc
     9721 gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta tctgaccgaa
     9781 tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag cagtgatttg
     9841 cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt gattttcccc
     9901 gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat ccattatcct
     9961 agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg aacggggatc
    10021 aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt atttattgca
    10081 aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt tgctgtacgc
    10141 ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac acaagaaaga
    10201 ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa tatttccaga
    10261 cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac agaaaataca
    10321 agacctgatt ttgtacttga actattagat attatttcag tcagaggtta tggcagagtc
    10381 cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact gttcggtgta
    10441 ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta gatatccaaa
    10501 ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat gatttcttag
    10561 atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta gaaaaagcag
    10621 tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct ttgaaccaat
    10681 caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc aaggaatccg
    10741 aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac gctgaaaaac
    10801 gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg acagatgcaa
    10861 cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa acacgagtat
    10921 tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa agtccggaat
    10981 gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa gtgatcaaag
    11041 aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca gacacaggag
    11101 tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg atcgtgcctg
    11161 gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc gagccgatga
    11221 gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga cctgcttttc
    11281 cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag tttgcactag
    11341 atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg ctgaacatgc
    11401 ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg aaagaaacct
    11461 tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga gaaggcgagt
    11521 ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg aacgaaggaa
    11581 aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc catttaggac
    11641 attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg tctggctatc
    11701 gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa gtattggcta
    11761 ataaag
//
LOCUS       DAYLZO010000087        11473 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.088, whole genome shotgun sequence.
ACCESSION   DAYLZO010000087 DAYLZO010000000
VERSION     DAYLZO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.088"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..1339)
                     /locus_tag="ACGVJG_002171"
     CDS             complement(<1..1339)
                     /locus_tag="ACGVJG_002171"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFJ6558093.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFS"
     gene            complement(1326..2357)
                     /locus_tag="ACGVJG_002172"
     CDS             complement(1326..2357)
                     /locus_tag="ACGVJG_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558094.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            2388..2609
                     /locus_tag="ACGVJG_002173"
     CDS             2388..2609
                     /locus_tag="ACGVJG_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFJ6558095.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(2774..3511)
                     /locus_tag="ACGVJG_002174"
     CDS             complement(2774..3511)
                     /locus_tag="ACGVJG_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFJ6558096.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(3664..5004)
                     /locus_tag="ACGVJG_002175"
     CDS             complement(3664..5004)
                     /locus_tag="ACGVJG_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFJ6558097.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(5241..6764)
                     /locus_tag="ACGVJG_002176"
     CDS             complement(5241..6764)
                     /locus_tag="ACGVJG_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFJ6558098.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(7142..7753)
                     /locus_tag="ACGVJG_002177"
     CDS             complement(7142..7753)
                     /locus_tag="ACGVJG_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFJ6558099.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(7876..8001)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(8083..8799)
                     /locus_tag="ACGVJG_002178"
     CDS             complement(8083..8799)
                     /locus_tag="ACGVJG_002178"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFJ6558100.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(8805..9374)
                     /gene="scpB"
                     /locus_tag="ACGVJG_002179"
     CDS             complement(8805..9374)
                     /gene="scpB"
                     /locus_tag="ACGVJG_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFJ6558101.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(9358..10128)
                     /locus_tag="ACGVJG_002180"
     CDS             complement(9358..10128)
                     /locus_tag="ACGVJG_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFJ6558102.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(10125..10496)
                     /locus_tag="ACGVJG_002181"
     CDS             complement(10125..10496)
                     /locus_tag="ACGVJG_002181"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6558103.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(10515..11402)
                     /gene="xerD"
                     /locus_tag="ACGVJG_002182"
     CDS             complement(10515..11402)
                     /gene="xerD"
                     /locus_tag="ACGVJG_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFJ6558104.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         3274 a         2473 c         1944 g         3782 t
ORIGIN      
        1 tagaaaaaag cttcctcttt ggcagaagtt ctgcttttgc tccatcaata tcacaacaat
       61 tctgcggctt ttcggagagt ttttcgccaa aataagctaa gatgaatttt ctgcggcagt
      121 tttcttcatt aatatagcga agcatctgct gcaggcggaa ttctttttct ttttcctgac
      181 gtttgagccg ttccacccaa ttttctggtt tttcagactg ctggatcagc tccatccatt
      241 ttttttgcag ctcatcaaag ggagcttgct ctgtggcata ttccaaataa agctcaaaac
      301 tttgtcgctg ttctctagaa agttgattaa agaaataatg gatacgttca tccccacttt
      361 gataaaaaag aattgctgca ctttcttcct gatctctgcc tgctcgaccg atttcttgta
      421 catagttttc tagactgtct ggcaaatcat aatggaccac ataacggata tcagatttat
      481 cgatccccat accaaatgca ttcgtagcaa tgagaaactg cagctgattt tttacaaact
      541 gctgttgaag ttgtcttctt tgagctgcat caagaccacc gtgatagtat ccaactgtaa
      601 aacgttccct gaataaatga tacaatcgtt cgacttcttt ttttgttgca caataaatga
      661 ttgccgctcc ttgtgctctt tccatgaagt gttcaagttc ttgttctttt tgctgcgttt
      721 tccgaacaaa aagcgagata ttttgccgat tgactgattg tctgatctct tttggtggtc
      781 gatcaaatag tacatgacgt atatctcttg ctactaaatc tgttgctgtc gccgttagtg
      841 ccagtgtgac cgggtttcct aaaagctttc ggatttttcc taactgttga tattctggac
      901 ggaaatcaac tccccattga gacacgcaat gagcctcatc tactacatat aacgcaatat
      961 tttgtttttt cagatgctct aaaagagaag gattagtcaa catttcggga ctcaaaaaca
     1021 aaaatttata ttgcgataga tgacggagga cgtatcttct ttcagattgc gaaagtaaac
     1081 tattgaaagc agctactcgt ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca
     1141 ttagagaaag cagcggtgac acgatgatga ccagaccttc cattaaatac cccgtcaatt
     1201 gatagcaaag gctttttccc gttcctgtag gcaaaatagc gagagtatct tctccatcaa
     1261 gcaacgcctg aataatttct tcttggccgg gacgaaatga atcaaatcca aaatattttt
     1321 ttaattcatt tattaacatt ctgtatcgct tcctttctca atagatagat ttgatacaaa
     1381 cgaaattctc cataatctaa ttgtccatct acattccatt cgctaaagcg ccactctcgc
     1441 acggaatcct tatttggttc caaacgttcg attgtttctg cagagagaat ccactcgtat
     1501 ggaaaatcat caaaaaacaa ggcccactca ataagatgat ctgttactgt tcctcttttt
     1561 aaatgacgga ttgccatgat ctggtcaatt gtctgaccat tcataaacat ctgcttggtg
     1621 atcatcatgc tttgattgaa attttgctgt aataatgaat cgatcaaagc gtgccaaagc
     1681 gaatctggcc gtttttcgat ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga
     1741 taaaccgctg actcatcatt gttttttgcc agctgataag gaagcagccc tgttcgctca
     1801 tttccagata attggttagc taaaaaatct gctgcttctt ctggtatctc cgaaaacagc
     1861 tgcaccaaac tctcatacgc agtatcaatc aaaatttcac gtggcaattg gctttcacta
     1921 agccattttt ttagttggaa agtataaaaa ggacttgtct ctgctggaag atagtcttga
     1981 atacccgttg ccagattaga aattacttga acagcaaatt taatgagccg ccaactagtt
     2041 tcccctgttc tgccatagcg gtcaaaacgc aaaccagtat gttctatttg atgttcggac
     2101 aacatatcag cccctgcagg cgttagcttt gcttcatttt catttatttc gatccaacct
     2161 tgctgcgcaa ttttttgcaa aatttgataa aacttatcct gcttcaattc tggaaaactg
     2221 ttatggataa acaaaagttc gtgggtaaat ccataaataa gtactgaagt cgttcgtttt
     2281 cccacaagga gttggtaaag agaactaatc cgcagcttgt gtcctgatga aaatagcgat
     2341 aaaataaaaa caatcatatt attctcctta agttaggatg gttagaaatg tcactactat
     2401 gtaaacttgt acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa gcgccagata
     2461 ttttcgatta tgatgaagaa ggaatcgttt tatttgccca agaaccagag gctcatcaac
     2521 agttcgtgat gccggaagaa gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc
     2581 gagcgatttt attagaaaat aaaatataaa tataaacgaa actttaaaaa cgtacactaa
     2641 gttctctaaa cttgaaaata agccgggatc tgtttctctt gttccgtgaa atcccgactt
     2701 attttgaaag attcatttgc tatctatagt taattgacta gttttttcac ggaccctata
     2761 ggacccattt atattatttc aactttttca gccagttttc caatgtattg acgatcacag
     2821 tttgctggtc tgcgttagaa atttctgcct ctccatctcc tttttgctca ccatagctgc
     2881 caaaacctcc atgatttccg ccattgattg atacatactc tgttctttcc ggtaaatact
     2941 gcttagcttc ttgataagct tcctgattca atactttatc ttttgtcgcc gtgatcgata
     3001 aaacaggaac agctgattct gtcaatgttc cttttttgtc gggataactt gccatgaaga
     3061 ataccccttt taaatgtttg tctgaatgat tcttattgac ataacgactt gccattacgc
     3121 cacctaatga atggccaccg atcacataat cggaatttgg aaaatcttct ttgaccttat
     3181 ccgctttgtt tccgcccatc actgctagat caagtggcat tttgacaata gcgactgaaa
     3241 agccgttttt tgctaaatct tgcgcccaaa tactataact ttctggctct acgagagcac
     3301 caggataaaa aataagtaat ggagactttc catgattctt tttctctcct tcaaaaaata
     3361 agtaatcttc tttgtttttt ccttctttta ccaacacttg cgcttctgac gatggttcgt
     3421 acgtattttg ctgtatatag taataaccgg ctccaccgag taatcctaaa atgatgacta
     3481 agccaattag tattcgtcta aatattttca ctttctccca ccttttctat ctgagtgacc
     3541 cctttttctt aatcaagtat gattatacct taaaaaaata agagagaaat gcaaactttc
     3601 cagcatcaaa taaaaaaaga cggaatatcc atttgaatat ccgccctttt tgtatgttta
     3661 tttttattta gcacataaac gatacacagc ttctgcataa atttccattg ctttatacat
     3721 actacttaat tcccatcgtt cattcgcctg gtgcataaag tcaggagtat cagggaacat
     3781 cgcaccgaat gccacacact ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa
     3841 cggttcagtc atgtctccag taatatcgcg atatgtcccc aatagatttt tcactaactc
     3901 actgtctagt ggcacgtata atgaatcaag gaagtcaaat tcttcataag tcaacccata
     3961 tttttctgct gttttagtta atttagctac taaatcatct ttcttgaatg taacagggat
     4021 tctcatatct acaccgattt ttgtttgttc tggtgtgatt tccaaacttg caaagttcat
     4081 cgttagctcg ccggattgtt catcttctgt tttgcccacg acagcttcac cagttgcatt
     4141 ttcttgaact aattttccta agaaattgat tgggccaaaa tcaaatactt ctgaaagagc
     4201 aattgccaga cgagaaatag cattcactcc ttgtgctgca tctttcgcgt ggatactttt
     4261 accaagaacg acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc
     4321 ttcttttact tcagaaagct tttctccaga gtaaggagct gcatcaggaa caacattcaa
     4381 cgctcctcca gcttttacag aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa
     4441 taagcctttt tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat
     4501 tccttcttct ttttcgttat atttttctaa acaacgccat aaattttctt catctgtacc
     4561 aaagataaaa cgaatacgtg tcttgaattc cacgccagca tcaatcaaag ctttgacggc
     4621 atacattgcc gcaatagaag gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc
     4681 gtcttttata gttggatcga atggttttgt ttcccaatta ttttcgtccc ctgctggaac
     4741 aacgtccata tgacacaaga taccgaataa ttcatctcca gagccaatct ctgaataacc
     4801 gtaatagcct tctggatctt taaatgttct aaatcctagc ttttcactga tttccaatac
     4861 tttatccaaa gcttcaatca cttttttacc gaagggatgt ccttgtcctg aatctgcttc
     4921 atctagtacg gaaggaattc ggatcaattc gctcaatgat tctaatgctt gttcatgatg
     4981 ttgttctgtc acaaattttt tcatgatatt atcctcctaa tatgttaaaa agggtcatgt
     5041 caaattcctc cgacacaacc tcttcttctc aaataatgac gttttaagcc tgctccatca
     5101 gcaagcagaa tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat ggagctattg
     5161 acttatccac gtgcttatcg atagccgttt atcggtttct tcattcttat gatagacctt
     5221 gtacctgtat ttgtctaggt ttagaacatt gctgctacac ctaggatcac acagatggct
     5281 acaaataata tcgcaattaa tttaccggta aatttccacc acactgaaac atcgaaacga
     5341 gcaattgcta cagcacccat tacgatagca gatgttggtg taattaagtt tactaaacca
     5401 gatgcagatt gataagcagt gattactaaa tccttaccga cgccggcaaa ttcacccata
     5461 ggtcccatga ttcccattgt tgcagcagct aagccggaag ttgaaggaat caagaatgac
     5521 atagggatat agaaaataaa tgtaataatg atgaatacac ttgaagataa tgaacttaat
     5581 ccttgttcgc cccaatgtaa gatggtgtct gtgattaatc cattgttcat aaccacttgg
     5641 ataccacgag caacagctac tacgattgct acaccgatca aatcagaagc accagctaaa
     5701 aattcagaga caaattcaga ctctttcatg ccataagtga ttgctactac gatggacatt
     5761 actaagaata acatagtaat ttctgggaag taccagtcac ctaatggaag catgctttta
     5821 cctagaaccg tacctagtac cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc
     5881 gtgaaattcg cattcaaggt tgtccaagga atcaaactga tgatcatgat accaaaagta
     5941 ataaagaaca atacattaac atgtttttgt tttttcgtga ttacttcttg acgaccgatt
     6001 gcttcaatat cgaaatgctt catatcttct ttacgttggt tgtatactaa tgattttgtt
     6061 ggatcttttt cgatctttga tgcataacga tacacatata caatagaaat agctaacaaa
     6121 ataacgaaca tcaagaatcg aagaccaata ccttcaccca tcgaaatccc aacagcttgt
     6181 gaagctacgc ctgttgcaaa aggatttacc gttgaagcta gacagcctac ttgagaccct
     6241 actaatacga ttgctacagc tactactgta tcaaagccta cagccatcat gactgggatc
     6301 agtaaagcat agaatggaat cgtttcttct gccattccgt aagtagagcc gcctaatgca
     6361 aataacagca tcaatactgg aataagcatt ttttcttttc ctttgaattt cttcacaact
     6421 gaagcaatcc cggcatctag tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca
     6481 accaaaatga agaaagagat tgggattgca gcaggtgttt ctttagttag ctcccccgct
     6541 ggtacaccta gcatcccatt gattggcgcc atgaaaatat cccacagccc ttgaggattt
     6601 tgttgtactc gttcatatga tccagcgacg atgttgcctg catcgtctac actatagtgc
     6661 ccagctggta taaaccaagt aagtacagcg attactgcaa taataataaa taagaccgta
     6721 tacgctgaag gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt cttcctttct
     6781 tttccgttag agacaacaat tttggctcta atgttatttt atcaaaaaga gtatcagaac
     6841 aaaaataata acacgcattt tgtaaaactt ttgttcaaaa agttcgtttt tccctattcc
     6901 ctcctctttt ttcaggtttt taaagctttc ttctttttca ttgtagcgtt tccttttttt
     6961 tataaacaaa cattttcgtc ttttaaaagg agaagaattt cacatttgca aagaattgat
     7021 tgatttttat gcataaatca aaaaaatact caaaacaaac aaataaaacg tttacattat
     7081 tgcatgtctt gtattttttt ccaaaaaaaa agaccaagaa atttcttgat ctttttgata
     7141 tttatttgac tgctgtatga cgttgttcga actgatgttg ttttcgactt aaccatggaa
     7201 gaagttgcgc atgcagcact aaaaagacag cactcacgat gaatcctttg atcaagttga
     7261 aaggaacgat cccaatcagt acatagtttg ctaaagacat gcccagcatc tgtgttgcgt
     7321 tcagccctaa aaacattaag taaagaggcg taataacgaa ataatttgcg atactcataa
     7381 aaatagttag agcgatggtt cctgtaatga cacctaaaaa tttgtttctt cctttatgct
     7441 gtttctttac actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt gataagaaac
     7501 tagcagtgtc tcctaccaaa ttatccggag aaaaacctgt agtcagcaga tgtaatactg
     7561 aacgcaagaa cgctgttgca actccggccg ctggaccgaa taagaacatg ctgatcaata
     7621 ccgagatatc actaaaatcg atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa
     7681 aatactgtaa aactaatgcg atcgcagcaa acatcgctac tgcaaccatt ttctgtacac
     7741 gggtgttctt catttgaaat cctcctgtta ggactcctct tcagcgaact ttttgacgtt
     7801 tctctccaaa tgaaaaaact ctattttcca gggaaaatag agttagagtt atttgatcaa
     7861 gcgtcgtcaa ataagctcta tcttctttat ccagactata ctgtcggtat cggaatttca
     7921 ccgattcagc tatttttttg caaaataggt cgtggactat aaccaccggt cgggaatttc
     7981 accctgcccc tgaagacgaa ccttatataa tttttgttta catattcatt atacatatct
     8041 attcaaaaaa cgcaacatct aacacttata aaaagtaagt gttcattttg agtggttcag
     8101 caaactgctg acttcttttt tgttcagttc acggtaatcc ccaggacgta ggccttgtaa
     8161 agtcagatct ccgtattttt cacgttttag tttttgcaca ggaagaccaa ctgcttgaag
     8221 catatttttc acttgatgat tacgtccttc gtggatcgtc aattccacga tactgtggtt
     8281 tgtcttagga tccactgata aaatttggaa tttagctgga gatgtttttc gtccttcgat
     8341 ctttattcct tttgtcaaag gtgcaagcat cgattttgta gcgatccctt ttacctttgc
     8401 aacgtaaact ttatccactt catgttttgg atgtgtcagg cgctgagcaa aatctccatc
     8461 atttgtcagt agcaagatcc ccgaagtatc gtaatccagt cgacctacag gatagatacg
     8521 ttcttttacc atcggcaaaa gatccgtaac aacttttctt cctttgtcat ctgcaacagc
     8581 agaaataact cctttaggtt tgtacaataa atagtaaccg tattcttctt gataaattgg
     8641 gacttcatcc acttccacag tgtcatgttt cccaactttt atccctaatt cccttacaac
     8701 ttgtccgttt acttttacac gtcctgcaag gatgaattct tctgcttttc gacgagaagt
     8761 gatgccagcg tgggcaatta ctttctgcaa tctctccatt acaattattc tcctttattc
     8821 attccatcaa agaatagatc tggtgacacg tcttcttcaa gtgaaatttc catttcttgt
     8881 gtatcaggca gttcttctaa tgatttcaaa ccaaaataat ctaagaagta gccagttgtt
     8941 ccatataata tcggccgacc tggtccttct actctacctt tttcttcaat cagtttatgg
     9001 gcagctagac gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc gatttcagct
     9061 cttgtgattg gctgtttata agcgatgatt gctaaggttt cgacggcggc ttgagataat
     9121 gtgtttgatg tcccttgcgc atatcgctta agtaagggag ccagctcttt tttagtcgtt
     9181 aatacataat ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa
     9241 gattctttta attctttcac taaacttgtg acttctgctt cgctgagatt taacagtccg
     9301 cttaattctt cattactgat cccttcctcg ccaacaataa aaagaagggc ttcaagttca
     9361 cttaatttcg tcatgttcgg tatctcctac tgctggataa agcataattg attcatagtt
     9421 ttctttttgt tgcgcaatga tctgcccttt tttcatcaat tctagcaaag ccataaaagt
     9481 agtgacaact tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc
     9541 atttcttttt atacgctctt cgataaatac cattcgttct tcgatcgttg tgtcatcggg
     9601 cgtgatcgtt ttttccactt tttgagaaaa tttcttacgt tctaagattt tctgcatggc
     9661 acggatcaaa tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag catccgctaa
     9721 cccatcgatg ttcataggtt cttttgtgta ataaagactt cgttcctctg ctttttctgt
     9781 cagtctttca gctgcatatt tatatttacg gtattctaaa agctgagtga ccaattcttc
     9841 tcgaggatct tcttcccata attcttcatc gtctgtgacg aattcttgga caggaagcaa
     9901 tgtcttgctt ttgattgcca tcaatgtagc agccatcact aagtattctc ctgcaacagc
     9961 tagctcgagt gttttcattg tatggatata attcatatat tgctccgtga tcgtagcgat
    10021 cggtatatca tatatatcga tttccatttg ctgaatcaga tgaagcagca gatcgagagg
    10081 accctcgaat acatctaatt taatattgat ctccgtcaac tcattcactc tttcttcttt
    10141 ttctttgcta gccattttcc aatgtaaggc tctgtgattt cagttgcacg gatggagaca
    10201 tccgggtact tttcctgtaa ttcatctaac atttgaaagc ccatattctc accgcgataa
    10261 gacggattca aagaaatgtc atggatgatc agctgatcgt tcgttgccca gtaaatacca
    10321 ataatccctt gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct
    10381 tcagattcgt acgtatcaat ctccttcaac aaagaagttt tacctgtcag tttcttatgg
    10441 aaactcaata gtcccatagc aattttacgg ttttctttcc ggtaatgtgt taacatcttc
    10501 tcaccttcct atgactaagc ccttggaaaa tgctgcttat aaacatccgt cattcttttc
    10561 ttcgtgatat gtgtataaat ctgagtcgta gagatatccg cgtgacctaa caattcctgt
    10621 actgtccgca aatcagctcc attttctagc agatgtgtgg caaagctatg ccgcagcgta
    10681 tgtggtgtta cttctttgta aatcccagcc tctcgtacta actgcttcag atttttccag
    10741 attccttgcc gagaaagacc tttcccatga ttattgacaa atacatgcat ttcagatgcg
    10801 tcctttacaa gtaatggacg tgcttcgtct aagtagcgtt ccagccattg gatcgcataa
    10861 tcacctaaag gaatgatccg ctctttatct ccttttccaa ctgtctgtac cagtccaaga
    10921 gaaagatgaa gatctcccaa tttcaatcca atcaattcac tgacgcgcat cccagtagca
    10981 tacatgactt ccaagattgc acgatctctt atccctaacg tttttgtcgt atcaggtgct
    11041 tcaatcagct tttctacttc tgtcagagtc aatgtacttg gtaatttctg tgctttcttc
    11101 ggcgtatcga tatgttgcat cggatcatga tcagtgatac gctcttgccg taaaaactga
    11161 tggaatcttc tgagacttga gatcatccga gtaacagttg ctgtcgcttt tttttcttca
    11221 tgcaacttct ccagaaaacg gatgactaaa aagcgatcga catcctgcca tgaagcaatt
    11281 ttctgctcct gtagaaaatg caagtactgt tccaaatcac gttcatagct ttgtcgtgta
    11341 ttggcggaca atccccgttc aatattcaag tagtggagat aatcgttgat ttgctctttc
    11401 ataataatcc ccctcatgcc tctctatcat atcagatacc gcttcattct gatagtcttt
    11461 ttccataatc tga
//
LOCUS       DAYLZO010000088        11439 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.089, whole genome shotgun sequence.
ACCESSION   DAYLZO010000088 DAYLZO010000000
VERSION     DAYLZO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.089"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..496
                     /locus_tag="ACGVJG_002183"
     CDS             <1..496
                     /locus_tag="ACGVJG_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFJ6558105.1"
                     /translation="EREGQVQSVDVTPKSVESNGEKVGQLGIKAPMNTGFMDKIIGGT
                     RQAFSGSLEIFKALGSLFTGFSLDKLGGPVMMYQLSSEAANQGVTTVISLMALLSMNL
                     GIVNLLPIPALDGGKLVLNIFEGIRGKPLSQEKEGILTLAGFGFLMLLMVLVTWNDIQ
                     RFFF"
     gene            564..2273
                     /locus_tag="ACGVJG_002184"
     CDS             564..2273
                     /locus_tag="ACGVJG_002184"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFJ6558106.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            2485..6843
                     /locus_tag="ACGVJG_002185"
     CDS             2485..6843
                     /locus_tag="ACGVJG_002185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFJ6558107.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            6988..7461
                     /gene="rimP"
                     /locus_tag="ACGVJG_002186"
     CDS             6988..7461
                     /gene="rimP"
                     /locus_tag="ACGVJG_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFJ6558108.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            7484..8659
                     /gene="nusA"
                     /locus_tag="ACGVJG_002187"
     CDS             7484..8659
                     /gene="nusA"
                     /locus_tag="ACGVJG_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFJ6558109.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            8681..8974
                     /locus_tag="ACGVJG_002188"
     CDS             8681..8974
                     /locus_tag="ACGVJG_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFJ6558110.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            8971..9282
                     /locus_tag="ACGVJG_002189"
     CDS             8971..9282
                     /locus_tag="ACGVJG_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFJ6558111.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            9295..>11439
                     /gene="infB"
                     /locus_tag="ACGVJG_002190"
     CDS             9295..>11439
                     /gene="infB"
                     /locus_tag="ACGVJG_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFJ6558112.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGI"
BASE COUNT         3964 a         2073 c         2538 g         2864 t
ORIGIN      
        1 cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag agtcaaatgg
       61 cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta tggataagat
      121 cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag ccttaggatc
      181 actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt accaattgtc
      241 ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc ttctttcaat
      301 gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga aattagtctt
      361 gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag gaatattgac
      421 attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga acgatattca
      481 acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga taactatttt
      541 gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca acattacgcg
      601 aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct ggatatatcc
      661 gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta ttggaaaaac
      721 tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg ctgatgcctg
      781 ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat ggaccaaact
      841 tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact cacgaagaaa
      901 cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct ttaaatcttt
      961 atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg cttcgcggaa
     1021 gagagtttat catgaaagat gcgtattctt tccatgcaac ggaagaaagt ttggacgaga
     1081 catataaaga ttacgaaaaa gcctacaccg aagtattcaa gcgttgtggc ttagaatttc
     1141 gctcgattat cggagacggt ggcgcaatgg gcgggaaaga ctcaaaagaa ttcatggcga
     1201 tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat gctgcaaact
     1261 tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag cttgaattgg
     1321 agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc ttttctgttg
     1381 aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct gtccttgttt
     1441 tggtacgcgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta tctgctgact
     1501 ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag tttggttctg
     1561 tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat gtccaagatt
     1621 tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat gtgaatccag
     1681 aacgcgattt cactgtactt tcttatgaag atcttcgttt tgtacaagaa ggagatcctt
     1741 caccagacaa caatggtgtt cttgccttta ctcgaggaat cgaaatcgga catatcttca
     1801 aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa aatggacgag
     1861 aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta tcagcaatcg
     1921 tagaacaaca ttcagatgaa caaggaatca actggccaaa aggaattgcg ccatttgatt
     1981 tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aaacctgacg aatgaactag
     2041 aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat gaacgcgcag
     2101 gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact gtcggtaaaa
     2161 aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg gttgaagtac
     2221 gtaaagaaga attggtgaat actttaccaa tcctcttgaa ccaaggcgaa taataagcgt
     2281 taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga tgattcgctt
     2341 ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa actcagaaaa
     2401 aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa ttgcattgtt
     2461 tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga aaaactgttg
     2521 gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc tggtgaaatg
     2581 gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctgca tttttccaaa
     2641 attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc ttttaaagat
     2701 attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga acagctcttg
     2761 caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact agcacagcaa
     2821 gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc aatcgacagt
     2881 acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga gttgttcgtc
     2941 agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca agcggaacga
     3001 gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat gaagcaagca
     3061 gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca atcgccagct
     3121 cttgaaggac ccatccacat ccagctagga cgaaatattc cagccgatga accaacgaca
     3181 ccaatgatca gtatcgttga agaagaacga agagtcacac ttgaggggta cgtattcgac
     3241 aaagaagtca gagaactacg ttcaaaacgc aagatcttga ctttgaaaat cacagattat
     3301 acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa aagatgaaca agtatttgat
     3361 gcgatcagtg tcggtagctg gttaaaggta cgaggaagta ttcaagaaga tacgtttgtc
     3421 cgtgatttgg tcatgaatgc gcaagatatc atagaagtca aacatacacc tcgcaaagat
     3481 tatgctcctg aaggggaaaa acgagtagag cttcatgttc atagcaatat gagtacgatg
     3541 gatgcaacaa acagcatctc tgatcttgtg gcacaagcag ggaaatgggg acaccgagct
     3601 atcgccatta cagaccacgg tggtgcgcaa gcctttccgg aagcacatag tgctggaaaa
     3661 aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg tcgtagatga tggtgtccca
     3721 attgcttata atgatgcaca tgaagcattg agtgaagcca catatgtcgt atttgacgtg
     3781 gaaacgacag gattgtctgc agtatatgac acgatcatcg agctagccgc agtcaaaatg
     3841 tataaaggaa atgtgatcga aagctttgat gaatttatcg atccaggaca tcctttgtca
     3901 cgaacaacgg tagatctgac tggtatcacg gatggaatgg ttcgaggatc aaagtcggaa
     3961 gaagaagtat tgcggatgtt ccttgaattt tcaaaagata caattttggt agcccacaac
     4021 gcggcttttg atatggggtt cttgaatact agctacgcaa ggtatggtat tcctgaagcg
     4081 gccaatccag tcattgatac gttggaatta gctagatatc tgtatcctca attcaaacgt
     4141 ttcggattag gtgtgttatc taaaaaattt ggtgtcagct tggaacagca tcaccgagca
     4201 atctatgatg ctgaagcaac tggtcactta gcatggatct ttgtgaaaga ggcaatggac
     4261 aatcataaca tgctttatca tgatcagcta aatgagcata tcggagaagg agattcttac
     4321 aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa atcaggcagg actgaaagat
     4381 ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg aacgcgtccc acgaatacca
     4441 cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg gctcagcttg tgataaggga
     4501 gaaatattcg aagccatgat gcaaaaaggg gtagaagagg caagaaaccg agcaaaattc
     4561 tacgattata ttgaagttat gccaaaagcg gtctatgcgc ctttgatcga gcaagaattg
     4621 gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt tggtagaaat cggaaaaagt
     4681 ttggataaaa tcgttgttgc aacaggtaat gtacattatc tgaacgaaga agatgccatt
     4741 taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc cgttgaatcg tcatagtttg
     4801 ccggatgtcc atttccgaac aaccgatgag atgctaactg catttcactt tttaggggaa
     4861 gaaacagcga aagagatcgt agtagaaaat acgaataaaa tcgcagatat ctgcgaagaa
     4921 gtgattccag tcaaagatga actttataca ccgaagattc caggttcaga agatgaaata
     4981 tcagaattga gttacacaaa agcaaaacaa atgtacggag atccgttacc tgagatcatt
     5041 caaaaacgat taaaaaaaga gttgaattct attaatggta atggcttctc ggttatttat
     5101 ctgatcgccc aaaaactagt gcataaaagt aatgaagatg gctatctagt aggttcacgt
     5161 ggctctgttg gctctagctt tgttgcgacg atgacaggaa tcactgaagt taatccgtta
     5221 gcccctcatt attattgtcc agagtgccaa tattccgagt tttttgaaga tggtacgtat
     5281 ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat gtggtgcacg cttaaacaaa
     5341 gacggtcatg atattccatt tgagacattt ttaggattcc atggagacaa agtgcccgat
     5401 attgatttga acttctcagg tgattatcaa gcagaagccc ataactatac gaaagtattg
     5461 tttggcgaag actatgttta ccgtgccgga acgattggta cagtcgccga caaaacagca
     5521 tatggctatg taaaaggata cgaaagggac aataatctgc agtttcgaag tgcagaagtc
     5581 gacagattag caaaaggcgc tactggagtc aaacggacaa ccggccagca tccagggggg
     5641 atcatcgtta ttcccgatta tatggatgtc tatgatttta cgccgatcca atatccagca
     5701 gacgatcaaa attcggaatg gaaaacgact cattttgatt ttcactcgat tcacgataat
     5761 gtattgaaat tagatattct agggcatgat gacccaactg ttattcggat gcttcaagat
     5821 ttatcaggga tcgatccaca aacgatccct acggatgacc cggaagtgat gcggatattt
     5881 gccggtcctg aagtacttgg agtaagtcag gaacaaattt attccaaaac agggacatta
     5941 ggtattccag aattcggcac tcgttttgtt cgagggatgc tagaagaaac gcatcctaca
     6001 acttttgcag aactactgca aatttccgga ctatctcatg gtacagacgt atggttagga
     6061 aatgcggaag aattgattcg acgaggagat gcgacactgg cggaagtaat cggctgtcgt
     6121 gatgatatta tggtttattt gatccatgct ggattagata gcgggatggc atttaaaatc
     6181 atggaaactg ttcgtaaagg acaatggaac aagatccctg atgaattgcg agaaacctat
     6241 ctatcggcga tgaaagaaaa taatgtgccc gactggtata tcgattcttg ttcaaaaatc
     6301 aaatatatgt ttccgaaagc ccatgctgca gcttacgtat tgatggctct tcgtgtcgct
     6361 tatttcaagg tttatttccc tattttgtat tactgtgcat acttttctgt acgtgcagac
     6421 gactttgatc tagtctccat gtgcaaagga aaagatgctg tgaaacaagc aatgaaagaa
     6481 atcacggaca aaggattaga tgcttccgta aaagaaaaga atcaactgac agtgctggag
     6541 ttagccaatg aaatgctgga acgaggattc aaatttggaa tgattgattt gtataaatcg
     6601 gatgccgtta actttgttat tgaaggtgat acgttgatcg caccatttag agctgtccca
     6661 agtttgggaa ccaacgtggc aaaacaaatc gttgaagcaa gaaaagacgg tccattttta
     6721 tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa cattgatcga atatatgaac
     6781 gacaatggcg tattgaaaga tctaccagat gaaaatcaat tatcgttatt cgatatgcta
     6841 taagcatctg ttttcctgtc tgcttgtaat tttaagcaga gtatggtata gttcttatag
     6901 gtaatgatac taactgtgag tgagcggatt tttcgctcac tctttttaat ggaattacgc
     6961 acttaatttt ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt cacagaaatg
     7021 gtgacaccga ttttagatga acaaaatttt gaacttgtcg aagtcgaatt tgtaaaagaa
     7081 ggaaaaagct ggttcttacg agtatttatt gacaaagaag gcggtatcga tatcgaagaa
     7141 tgcgcctttg tcagtgagaa actgagtgaa aaacttgatg cagcagatcc agatccgatt
     7201 cctcaagctt atttcctgga ggtatcctcc ccgggagcag agcggccatt gaaaaaagaa
     7261 agcgactatg aaaaggcact tggtgattat gtccatgtct ccttatacca accagtagat
     7321 ggcgaaaaac aatatgaagg atttcttcaa tcatttgatg ctgaacagct tacattgaaa
     7381 atacgcataa aaacacgaga aaaagaattg acttttgacc gaaaaaatat tgcaaaagct
     7441 cgcttagcta tccaatttta atcacggaga ggaatcagaa aaaatgagta aagaaatgtt
     7501 aaatgcgtta gacgctttag aagctgaaaa agggatctca aaagaaatcg tgatcgatgc
     7561 tttagaagca gcattggttt ctgcatacaa acgtcactat ggccaagcac aaaacgtcga
     7621 agtggaattt gatcagaaaa aaggaaagat ccatgtctat gcggtaaaag aagtaacgga
     7681 agaagtgatg gattcacagt tggaagtctc attgaaagat gcacttttaa tcaatccagc
     7741 atatgagatc ggtgatacga tccgttttga agtaacacca aaagattttg ggcggatcgc
     7801 tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt gaagcagaaa gaacgattat
     7861 ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa ggaatcgtcg aacgccagga
     7921 taaacgttat atttatgtca atcttggtaa aattgaggca gttctttcta aacaagatca
     7981 aatgccaaat gaattttatc aaccacatga tcgaatcaaa gtttatgttt cccgagtaga
     8041 aaatacgtct aaaggtccgc aagtatttgt tagccgcagc catccagacc tattgcgccg
     8101 cttatttgag caagaagtac cagaagtcta tgatggaatc gttgaaattg tcagcatcgc
     8161 ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca aatgatccaa atatcgatgc
     8221 agtcggtaca tgtgtcgggc caaagggaca acgagttcaa gcaatcgtca atgaattaaa
     8281 aggcgaaaac atggatatcg tcgaatggaa tgaagatcca gctgtattca ttgcaaatgc
     8341 attgaatcct gcacaagtca tggatgtgat cttcgatgag acaaatccaa aggcctgcac
     8401 agttgtcgtt ccagactacc aattatcgct agcaatcggt aaacgcggac aaaacgcacg
     8461 tttagctgct aaattgacta accataaaat cgatatcaaa tcagaatctg acatgacgga
     8521 attttatgaa aatcaagaac aacaaaaaga aacagaagaa ctgcatgatg aagcaatcgt
     8581 tcagtcagat ttaacagacg atgaatatga aacaattgca ttcaacaatg aaacggtcga
     8641 agaaaaacca gaagcttaac tttagtaagg aggcaaagag atgaaacaaa ggaaaattcc
     8701 tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa aaagagatgg tccgaatcac
     8761 ccgttcaaaa gaaggggaag tatcgatcga tccaactgga aaaatgcctg gacgaggagc
     8821 gtatgtctca cttgagccgg aagaagtaca acaagcttgg gataaaaaaa ttctcgatcg
     8881 agtactcgaa acaacattaa cagatgagtt ttatcaagaa cttcttgact atgtcactca
     8941 ccaaaaagca cgtaaagagt tatttggaaa atgaatacag ttaatcggca aaaagccatg
     9001 aatctcatcg gtctagcgat gcgcgcggga aaactaatca cgggtgaaga actgaccatt
     9061 gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg caaccgatgc aagcgagaat
     9121 acaagaaaaa agataaaaga taagagttca tattatgaag ttccttgctt tgagctattt
     9181 tctgaagcag aaatcacgca gatgatcgga aaacctcgca aggtattcgg aataatggat
     9241 gccggatttg cgaagaagac caaggagcta attgaaggtt aggaaggtga ttgcatgggc
     9301 aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat ctagtaaaga cgtcgtggaa
     9361 aaagcacaac aacttggcat cgatgtgaaa aaccatatgg gtgcgattac tagcaatgat
     9421 gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga aacaacaagc tgcacaacaa
     9481 gcaagcagaa aaccagccaa tcaacagccg aacgatcaac gtaaaaaaga aacaacgaac
     9541 aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc gcggtcaagg gagcggtcaa
     9601 gtgaatcaag gaaaaaaacc aacaacaaac caaaataata cgggaaatac cagcagccaa
     9661 aatcgtcaag gaacgagcca aggcggcaat caaaaccgta ctaacaataa caacaaacca
     9721 aacaataaca atcgcggcaa atttaataat aataaccgta atcgttataa taaaaaaggt
     9781 aaaaaaggca aacagcaaca atcaactaaa ccagctgtac caccacgcaa attccgtgaa
     9841 ttgccagatg tattagagta tactgaaggt atgaacgtag cagatattgc gaaaaaaatt
     9901 catcgtgagc cagcagaaat cattaaaaaa ttatttatgc ttggtgtcat ggtcaatcaa
     9961 aaccaagcac tggataaaga tacgattgaa ctgttagcga cggactatgg catggaacca
    10021 caagaaaaag tccaagttga cattgctgat atcgataaat tcttcgaggc agaagaagtc
    10081 gatccagata aattagtctc acgtccgcca gtcgtaacga ttatgggaca cgtagaccat
    10141 gggaaaacaa ctttattgga tacattacgc cattcacgtg taacaagcgg cgaagccggc
    10201 ggaatcacac agcatatcgg tgcctaccag atcgatattg atggtaaacc aatcactttc
    10261 ttggatacac caggacatgc ggcctttaca agtatgcgtg cacgtggagc aagtatcaca
    10321 gatatcacga tcttagttgt tgctgcagat gatggcgtca tgccacaaac agttgaagca
    10381 atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg ctgtaaataa aattgataaa
    10441 ccaggtgcaa accctcaaca tgttatgcaa gaattaagtg agtatgagtt aattcctgaa
    10501 gcatggggtg gagaaacgat ctttgttgag atctcagcga aattcggcca aaacatcgaa
    10561 gaattgctgg aaatgatcct gttagtcgca gaagtagaag atttaaaagc agatccaaca
    10621 caacgcgcaa tcggaacagt gatcgaagca cgtcttgata aaggtaaagg tcctgtgtca
    10681 accttacttg tccaagaagg aacacttcat gttggtgacc caatcgttgt cggtaatact
    10741 tacggacgtg tccgtgtaat gacaaacgat cttggccgtc gcgataaaga agcaggacct
    10801 gcaacgccag tagaaatcac tggcttgaat gatgtacctc aagcaggtga ccgtttcgta
    10861 gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag aacgcgcgaa acgtgcatta
    10921 ttagaaaacc gtgctgccac aagccgtgtg actttggata acttatttga aagcctgaaa
    10981 gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag atgtacaagg ttcagcagaa
    11041 gcactggctg catcattgaa gaagattgat gtggaaggtg tccgtgtaaa aatcgtccat
    11101 tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag cagcagccag caacgctatt
    11161 atcatcggtt tcaacgttcg tcctacaccg caggcaaaag tgcaagctga tactgaagaa
    11221 gtcgatattc gtcttcaccg tatcatctat aaagccatcg aagaaatcga aactgcgatg
    11281 aaagggatgc tagaccctga atttgaagaa aaaatcaccg gtcaaatgat cgttcgtgaa
    11341 accttcaaag tttctaaagt tggtacgatt gcaggtgcct atgtaacaga aggatatatt
    11401 cgtagagata gcggtgtccg tgtaatccgt gacggaatc
//
LOCUS       DAYLZO010000089        11423 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.090, whole genome shotgun sequence.
ACCESSION   DAYLZO010000089 DAYLZO010000000
VERSION     DAYLZO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.090"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..918
                     /locus_tag="ACGVJG_002191"
     CDS             <1..918
                     /locus_tag="ACGVJG_002191"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558113.1"
                     /translation="LFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQL
                     KYGNETQIKNCDIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLI
                     SGFYNVDKGQIKIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPAS
                     KVFDALRLAGCESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDE
                     ASASIDPDNEYELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNE
                     LMAKHQVYKQLVTNYENANDWRLDNEKFL"
     gene            878..2644
                     /locus_tag="ACGVJG_002192"
     CDS             878..2644
                     /locus_tag="ACGVJG_002192"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558114.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            3167..3436
                     /gene="rpsN"
                     /locus_tag="ACGVJG_002193"
     CDS             3167..3436
                     /gene="rpsN"
                     /locus_tag="ACGVJG_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFJ6558115.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            3451..3630
                     /gene="rpmF"
                     /locus_tag="ACGVJG_002194"
     CDS             3451..3630
                     /gene="rpmF"
                     /locus_tag="ACGVJG_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFJ6558116.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            3662..4039
                     /locus_tag="ACGVJG_002195"
     CDS             3662..4039
                     /locus_tag="ACGVJG_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558117.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            4060..4209
                     /gene="rpmG"
                     /locus_tag="ACGVJG_002196"
     CDS             4060..4209
                     /gene="rpmG"
                     /locus_tag="ACGVJG_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFJ6558118.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            4233..5216
                     /locus_tag="ACGVJG_002197"
     CDS             4233..5216
                     /locus_tag="ACGVJG_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFJ6558119.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            5246..5368
                     /locus_tag="ACGVJG_002198"
     CDS             5246..5368
                     /locus_tag="ACGVJG_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFJ6558120.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            5386..6909
                     /locus_tag="ACGVJG_002199"
     CDS             5386..6909
                     /locus_tag="ACGVJG_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFJ6558121.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            6933..7172
                     /locus_tag="ACGVJG_002200"
     CDS             6933..7172
                     /locus_tag="ACGVJG_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558122.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7365..7826)
                     /locus_tag="ACGVJG_002201"
     CDS             complement(7365..7826)
                     /locus_tag="ACGVJG_002201"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFJ6558123.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(8021..8239)
                     /locus_tag="ACGVJG_002202"
     CDS             complement(8021..8239)
                     /locus_tag="ACGVJG_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558124.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            8357..9973
                     /locus_tag="ACGVJG_002203"
     CDS             8357..9973
                     /locus_tag="ACGVJG_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFJ6558125.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(10214..11423)
                     /locus_tag="ACGVJG_002204"
                     /pseudo
     CDS             complement(10214..11423)
                     /locus_tag="ACGVJG_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         3916 a         1729 c         2259 g         3519 t
ORIGIN      
        1 ttgttttttt taagtggaat tatttttgtt tctttcatga gaataatgta tgtaagtatg
       61 cacttgtttg aagcaagata tgcagtcgaa acactagact ccctttattt agaaatgaag
      121 caagaccaac taaaatatgg gaacgagaca cagattaaaa attgtgacat tgcatttgaa
      181 aatgtcagct tttcatacaa agaagagtgt gttttagaaa atttcaactt agttttagaa
      241 caaaaaagaa tttatgcatt agtaggaaaa tcaggaagcg gaaagagtac gatagctaaa
      301 ctaatttcag gtttctacaa tgtggataag ggacagatta aaattggtgg taagccttta
      361 aatcactatt caaaagacac aattattaac acaattgctt ttgtctttca agatacgaaa
      421 ctattcaatc agagtattta cgataacgtt tctatagcaa atcctcaagc gccagcaagt
      481 aaagtatttg acgcgctaag attagccggt tgcgaatcga ttttagaaaa atttcccgaa
      541 agagagcata ccttaattgg tagtaaagga gtttatctgt caggaggaga aaaacaacgt
      601 atagctattg ccagagcaat tttaaaagat gcaaagattg ttattatgga tgaagccagt
      661 gcatctattg atccagataa tgagtatgaa ctacaaaagg cttttcaaca tctgataaaa
      721 gaaaaaacag ttattatgat agctcatcga ttatccagca ttaaatctgt agatgaaatt
      781 cttgtcttgt cagaagggaa aatagtagaa cgtggaaatc ataatgaatt aatggctaaa
      841 catcaagtgt ataaacaatt ggtcactaat tatgagaatg caaatgattg gaggcttgac
      901 aatgaaaagt ttttataaaa acaaatttgc tttaagtgat cagggcgcag aagctttaac
      961 taaagcttct atttctagtt tttttgtata ctgcattaat atggtaccag cattcattat
     1021 aatgatgctg atagacgaat tagttttaga aaatacaaag ccccgttggc tttattttgc
     1081 cgtatcgttt gttactttgc ttttcatgta ttggttgtta gatagggagt atgaaaactt
     1141 atataatagt acttataaag aaagtgcgca tttaagagtg caaattgcag acgatttgtc
     1201 aaatttacca ttatcctatt tttcaaaaca taatttatca gatttatctc aaactatcat
     1261 gtctgacgtt gaaggtattg agcatgcgat gagtcatgca atacctaaag ccggcggtat
     1321 ggctctgttt ttccctttta tttcagtgat gcttttggtt ggtaatgtca aaatgggatt
     1381 agctgttatt ttgccaacgt tatttagttt tgtcttaatc ttgttatcaa agaaatccca
     1441 aacgaaagcc aatactaaat attacgatac tttgagagaa aactcggaag aatttcaaga
     1501 aactatagaa ttgcagcaag agattaatag ctttaatcta tctaaaaaag ttcaagacag
     1561 acttttcaaa aaaatggaag agagtgaaag gattcattta aaggtagaat taagtacttt
     1621 ttcagtcatg gccttatcct ctattttctc atatgttagt ttagcagtag tcattctagt
     1681 aggtgttcac ttactgttaa cgggtgaagt gactatactc tacgtcgttg gttacttact
     1741 agccgcaata aaaataaagg attccttcga ttctatgaaa gaagcagtgc ttgaaatatt
     1801 ttatttagct cctaaaatac agcgaattag agctatgaag gaaaccagta tccaagaggg
     1861 atcagatagt cctctgaaat cttttgacat tgagttgcga gatgtctctt tctcatatga
     1921 taacaatacc ccaattttag atcatatctc ttttacggct aaacaaggag aagtgactgc
     1981 gttagtaggt gctagtggtt ccgggaaaac tagtatcttg aaattagtgt ctagactgta
     2041 tgattatgat gaaggctgta ttttaattga tgggtatgat attaaacgtg tatctccagc
     2101 ttctttgttt agcaaaatcg ctattgtttt tcaagaggta actttgttta atacaagtat
     2161 tttggaaaat atacgtattg gaaatagtca agcgtcagat gaggaagtga aaaaagcagc
     2221 tcgtttagct aactgcgaag actttattga aaaactacct gacggttatc atacattggt
     2281 cggagaaaac ggaagttcac tttctggagg agagcgacaa cgcctatcca tagcacgggc
     2341 ttttttgaaa aatgcgccaa ttttaatttt agatgaaatt actgccagtt tagatgcgga
     2401 aaatgaaaaa cgtattcagg aaagtttaaa tcgtttaata caagataaga cagtactaat
     2461 tatatcgcat cgcttgaagt ctattgaaaa ggtaaataaa attgtagtga tggatcaagg
     2521 aaaactagtt gatcaaggta ctcataatga gttgtatcga aggtcagaaa tatacaaaaa
     2581 tctcataaag aaaactaaac tatctgagaa attcgtctat gaaaaagaag ctcaatcacg
     2641 atgatgaaat aggctttaaa ttaacctaca atttttggga tatagttata attctttttc
     2701 aaaaagaata attttattaa acagcatcta attttgaata cacgaaagaa tcagtatgat
     2761 tgaattagtt cacttaatgt ctattcttat aatataagga ataatcaaag ttttaaaaaa
     2821 ttggcgataa accagttaaa cataaaagat tgaataaaaa gttatagttt ctttcatatt
     2881 gccgttttaa gtagaggaaa agctacttaa aacggtattt gtgtttgatt ggactaataa
     2941 aagtgaattc ttatttttca gagcctaaac tgcatattct tttaaatgat ttgattatct
     3001 ctatgtaccg ataaattcgt tttcaaatag ttatctattc atatgagttt tatttcatgt
     3061 tatttattgt tgacaagagc aactcttgtt ggtaaagtat cagtgtcaaa acgtaacgat
     3121 tacgatttga ggtgtctaaa tcaaaataaa aggagtataa aggtatatgg caaaaaagtc
     3181 gaaaattgct aaagcaaaga aacaaatggc gatgattgaa aagtatgcag ataaacgtca
     3241 ggaattaaaa gccgcaggag atcgcaccgc tctcgctaaa ttgccaagag attcaaatcc
     3301 gaatcgtctg aggcttcgtg atcagacgga tggtcgtcca agagggtata tgcgaaagtt
     3361 cggtatgtcc cgtattaagt ttagagaact tgcccatcaa gggttgattc ctggagtaaa
     3421 aaaagcaagt tggtaaaagg aggaaatatc atggcagtac cagcaagaaa aacatctaag
     3481 gcgaagaaac ggttaagaag aactcatcaa actgtagtaa aaccagaaat ttcatttgac
     3541 gaagcgaatg gtgattatag acgtagccat catgtatctt taaaagggta ctataatgga
     3601 aagaaagtaa ttaaaggagc ttcaaaatag tttaatatat aacttttagg agggaatcaa
     3661 aatggaaatt atttacccac ctttagttga agaaggatta aagtattatc ttgaaacgac
     3721 ccagcaatcg ctagataaat gcacttttta tcgttcgatg gtagagagag gaattattac
     3781 tgaaacgggt ttaccgaccc aacaggcaat tgaaaatgga ttagtcaaag attactatga
     3841 ggaccaaggc ttatcatttg atgatttttt aagaatctat ccaatttttg aagagtatga
     3901 cgaggagtta tttcaatgta tcgatggtta ttgggagata cctgttgata tgaaagaaaa
     3961 tttagtttca caattggaat ctggcgagtt aacttttgaa gatgcacaac aaattcaagc
     4021 atatcttgaa gatcgataag tattgaagga ggaagattta tgcgtcaaaa tattattttg
     4081 gaatgtgtcg aaactggaga acgtctgtat ctcaccagca aaaataaacg gaataaccct
     4141 gaacggttag aattaaaaaa gtattcgcca aaattgcgca gacgggcaat ttttaaagaa
     4201 gtgaaatagc taagtggtaa aaaggtgaca aaatgggcat accagttaca atcgtttctg
     4261 ggtttttagg ggctggaaaa acaacgctaa tcaatcaagc cttgcaagtc tcaccctacc
     4321 ccagggaaga aattattata attgaaaatg agtttggtga agttgggatt gatcacaaat
     4381 tattagttca tagcgaggaa cgggtgttac aactaaataa tggatgtatg tgttgcagtc
     4441 ttcgcagtga tttattagca atattgattt ctttactgga aacaatagaa gaacatcaac
     4501 aaccaatttc acaaatcatc attgaaacta caggtatagc agatccccaa ccaatcattc
     4561 aaacactttt aacagcgcct caactaagag gaagagtata tatcgatagt ttattgactg
     4621 ttattgatag tgacagtttt gaacttattg aaagagaacc acaagccctt aagcaacttg
     4681 tcatggcaga tcgcattttt gtgtctagaa aacaaaactt gaatttatct gcactaaaaa
     4741 aattacagag agaaatcaaa agtattaacc ctttatccga tattcgaatg ttcacacata
     4801 gtgaaacgat tcaaaaaaag gatttttata atttgaaaaa atttaatcat cccttagttt
     4861 ttgaaagtga ttcatcagaa aatcactata atggcaaata ctatcatcaa caccatcaag
     4921 tacatgagtt taaatcaatt ctcttaactt cagatcaaga cataaaaaaa gattttttac
     4981 tccaatggat tgactggctg attttaacca accaagggag cctgtatcgt tttaaaggat
     5041 tgattgcttt acaagagcag gattttatag ttgccatgca agaggtaaac gaacaggtta
     5101 attttcaata caccactcga caaaaagacc aaaccactat tattctaatc ggaaaaatgt
     5161 tggatgaaga aaggatcaaa cagagttttg ataaattact ggcggaaagc aaataatgat
     5221 aagtagtgat tgggggtaat ttctaatgga gaaaacggat atatctagtg catatcgacg
     5281 acttaaaagc ccgaatatta aaactagaaa aagggcatta aaaattatca aagaacataa
     5341 acgaaataaa atgaagaaat tagcataatt aaggaaggaa aaagaatgaa gaaaatcagt
     5401 gtaggactta ttgggatagc agctctagga ttattgggag cttgctccag cactaatgat
     5461 gcaaaagtat cgaatgataa agatggaaaa ttagaaattg taacaacatt ctatccaatg
     5521 tatgatttca ccaaaaacat cgtcggcgat gaagcgaacg tggatttaat ggttccagcg
     5581 ggatcagaac ctcatgacta tgagccctca gctaaggata tggcaaaagc tcatgatgct
     5641 gatgtttttg tatatcataa cgaaaacatg gaatcatggg taccaaaagc taaagaaagt
     5701 tggaagaaag ctgggcctaa tgtagtagaa ggaacaaaag acatgatttt attgccaggt
     5761 agtgaagaag aagatcatga tcacggagaa gaagatcacc atcatgaact agatcctcat
     5821 acgtgggttt cacccaaaat ggcaattaag gaagtttcaa atatcaagga tcagttagtt
     5881 aaattgtatc cgaaaaaagc aaaagtattt gagacaaatg ctgaaaagta tttgactaaa
     5941 ctgaaacggt tggatgctga ctataccact agtcttaaag aagcgaaaca aaaaagtttt
     6001 gtgacgcaac acgcagcctt tggatattta gctttggatt atggattgaa acaagttcct
     6061 attgctggac taagtcctga agaagagcca tcttctggtc gattagctga attaaaagag
     6121 tatgttaaaa aaaataagat caattatatt tactttgaaa agaatgctaa tgataaaatt
     6181 gcaaaaactt tggccaacga agctgggata aaattggaag tactgaatcc tcttgaaagt
     6241 cttacaaaag aacaaatgga taatggtgaa gattatgttt cagtaatgga agacaatcta
     6301 aaagcgttag aaaaaacaac tatggttgct ggtgaagagg tagttccaga aaaagaagcg
     6361 aaggatgaaa agacagttgc taatgggtac tttaaagatg ccgatgtgaa ggatcgcgaa
     6421 ttgagtgatt atacaggcga atggcaatct gtttatccgt tgttaaaaga tggaattttg
     6481 gatgaagttt tcgattacaa agcaaaaatc aataaagaca tgacagctgc cgagtacaaa
     6541 gattattata ctactggcta taagacagat attgatacta tcaacattaa agacaacacc
     6601 attgattttg tagtcaacgg tgaacatcat caatacacat acaaatatgt tggatacaaa
     6661 attttgaatt atgaaaaagg aaaccgtggt gttcgtttta attttgaaac tgatgatgca
     6721 ggtgctggtc gttttaaata tattcaattt agtgatcatg gaattgcacc aagtaaggca
     6781 gagcatttcc atattttctt tggtggagaa agtcaagaaa aactctataa tgaattgcat
     6841 aattggccta ctttctatcc agcttcattg tctaaacatg aaattgctca agaaatgatg
     6901 gctcattaaa attcaatttt tggaggaaat acatggtaag aaaagtatta ttgtctatgg
     6961 attccaagaa tattgatgct tatcggcgtt atcgatttgc aaagcaaatg agattaaatg
     7021 ggagtacatt agctgatatt gctgcaactt tacatgttag tattgacaca gttcatagag
     7081 tattaaaacg ggatcctgat tgttgtttta tcaaggtcaa tactggaaga agtagacgtt
     7141 tagcaaaacg gcataatagc ttggccaatt aattgataat accagagtag atcatttttc
     7201 ccttatttgg atttgatctg ctttttatta tgtcttgaat tgtcaaatca agcgcaacac
     7261 tttatcaaag aagacttcat aaggtgttct ccaatctaaa cactttctag gtctgttatt
     7321 cagtttttca atcatctgat ttatcgttga gttatctacc gtagctatct ctttcgattt
     7381 tggcaagtat tctcgaatga gtctattcgt attttaattt gttcattgtt gccaagggca
     7441 tgaggatctg gaaaataaaa gggcatatta ttcaatgctt ctgtaactgc gaaatgattg
     7501 gagaattctt ttcctctatc tggagtgatc gttttgaccg cctcatttgg aatactgctt
     7561 aaaagttcaa tcattttctc gctcacataa gcggctttct ttctggggac cttctttcct
     7621 gctaaaagga agcatgattt tctgtctgtc aaagtcacta aacaagtacc accagttttg
     7681 ccagctacaa tatcggcttt ccaatgacca aaatcacttc tatcatctgc ggattttgga
     7741 cggccatgga tcgaatgagt aaggcaaatc tttccacgag actcgacatg attttttgta
     7801 tgtcttgttt ttcctcgatg gtgcaatgaa cggatcaggc ctttattgcc gtgtgagagg
     7861 ggttctgtat cgaaaagatc attgcaaatt gcacgttaga tcgtgataaa actaattgaa
     7921 aagtccatat gttcatgctt caaacgattc gagatttcct ctggcgacca ctgaaaatcg
     7981 acaaataaat gacgaacaat tgaccaaact cacggatcat ttagaatgtg ataacgtccg
     8041 catttttttc ttcgataagc ataatttttt tccgctttgg aaggtgaata gtttttttct
     8101 tgtttcttct cagttcatgt aaaatagtaa acgggctatg ctgaatgtcg ttgccaatta
     8161 tactaataga aaatccttct tcgtgaagca gaaagattct ttctcgttcg agtatagtaa
     8221 gatgacgata ctggctcata attattttcc ttctaggagt gttctcgaca aacttatact
     8281 agttggatat cgattatgac tcatttttat gtgttgcact taaagtgtaa attcaaggta
     8341 tagaaagagg ttccctatga aaaaaatacc tattacaatc ttaactggct atcttggttc
     8401 aggaaaaacg atacttctaa atcatattct gtacggtgat cacggaaagg aaattgctgt
     8461 cattgtaaat gacatagggg aagtaaatgt tgatggggta ttagtcgaca agaaaggttt
     8521 taaaagaact ggagaaaaac ttgtttcgat gccgaatgga tgcatttgtt gtacattacg
     8581 cgaagatttg attgttgaac tagatcaatt agtgcaattt tctgacttga atcaaattgt
     8641 tattgaagct tcaggaatca gcaaaccaat tccaattatt caagcaatta tattagctaa
     8701 aaacccttta gacattgatt tgacccaacg attagagatt ggtgccatta ttatagtagt
     8761 ggatgtaaaa cgtttttggg ataatttcga aacgggtgaa aagattcagg aaagaaaagc
     8821 tgaaggtaat gtagttgaag aagaacagga tattcgtggc ttgttaattg atcaattcga
     8881 gtgttgtaac atattacttt taaataatag tgacttagtg tcaaccaaga cgatttacaa
     8941 aattaacggt ctaattgcta agttacagtc caatgccaaa ataattagga caattaaagg
     9001 tgaagtagac ttatcgaaaa ttctaaatac aaatcttttt aatatagatg agacttccta
     9061 ttcagtgggc tggttcaaag agttagagct tggctatcaa aatcatattc ctgaaacaga
     9121 agaatatggt atctcaagtt ttatctatcg aaatcggtta ctccttgacg cacaaaaatt
     9181 tgcccatctt acggataaaa attttcctgg caatattaca agagcaaaag ggtttctttg
     9241 gtttgcccaa catagtgatt tttcgacgat ctttgaaaca tcgggtagct ataaaagtat
     9301 tgaatcatta aattattggg tagctagttt acctaaaatt gagtgtcaaa tgattataca
     9361 aaaaaatccc gaattgaagg aagcctggca tccgttgttt ggtgaacgca ttaataaact
     9421 agtttttatt ggtatgaaga tggatcaaca atgtattaag gaacaacttg agggttgttt
     9481 aactgattct cttaatgcag ataatttccg aaaaatttca ccatttccat ggaaaaagtc
     9541 taatatgttt agccagatat cgttaatgaa atttttaaga aaagctggtg tcaagatagg
     9601 cgatgtcata attattggca ttttgattat tacctctttt attcccttat ttgtattctc
     9661 tatagaaaag gagaatgaaa caactgaaga tgctatcaaa tatgccatcg ttcgaattga
     9721 tggcaaggaa atggatcgct tcgatttgga taagatcaat cataaactcg tgaattatca
     9781 tcctgcgaag ggacaatata atattgttga gattaaagat ggaagaattc gagttaaaga
     9841 agataatagt cctgatcaaa tcgcggttag aactggatgg atctcaaaac ccggtcaaac
     9901 gagtatttgt ttaccccata agttagttat tagtattgag aaaaaagaaa gcacagatta
     9961 ctacatttac taatctggaa atagatgagg aggtttttaa taagaaaaag atattattat
    10021 taataatagc aggggcttta ttaagtgtgt taagcgcttg tggacaagaa aaagaagcgg
    10081 tgacaacagg cacaagtatc tttaacggga cctgcaatgg tttagttaac agtcggttag
    10141 attacttatt ttggctcttc gtcaatgagt gttggtgaag aaattcaata gaataatcga
    10201 gatactcaca cggctagaca gctatcttgt ctagccgtgt tgttgttctg gctttagtca
    10261 gttgggtagt attcttagtt ttcttaaaat attttcctcg atataaaaag attcgttctc
    10321 ggtagttttg aaagtttcta tagccatagg caattcgttt tagtactttg atatggttat
    10381 tcaaacattc tatagggccg ttggaatagg gaacgcgcaa cgctcgtttg atttctgttt
    10441 ggtatttctt gaaggtcgtg aacactggtt ggtagtgctc tggcaactcc ttgtagtctt
    10501 cttttaggag cgcgacgaat tcaggaacat cttgtcttct gataacggaa agaaaatcct
    10561 ggtagacttc gtagcctcgc tttaaggaat catcataaga taatagccga tcgactatct
    10621 ctgcttctgt cagatgatct cgaaatgacg gacgccagta gcgatgctga cctttcaatt
    10681 cccacgcatt cttttgaagt agtttccagt atttctttaa gtgcttcgac cgacgatccc
    10741 cttttttaaa ggtcttcatt acttgaattc ggtggttggt aaaagcccga ccaatgtgtt
    10801 ggctaatatg aaagcgatcg gcgacgattc gggcattcgg aaaacactct ttcaccaatg
    10861 tgcgataggg tgtatagaaa tctgaaacca ctagacgcac ttgttcacgg acttttatag
    10921 ggtagcgcaa gaaatagtcg cgtaaaggat tgagttgccg attttccaca atatcaatca
    10981 gttgatgggt cttgccgtcc atcatgacaa agctcatggc tcccgagaca tttttcactg
    11041 atttgaattc atcgaaacac accacttctg gtaacagctg gctcaaagaa atggttttag
    11101 gccgaaggct gcgaagtatt cgaatgacag tggtcggaga aatgtgcttc atacgtgcga
    11161 tcaaagacat agaaagaggt tcgcttaata gctcaagaat ggcttgtttg actctgcgag
    11221 caatggagca gcgacgatca ctaactgaat cttctgctaa aaaagtcttt ccacagtgtt
    11281 tacatttgaa gcgctgtttg ttaatccgca gaatggtctt gtagttactg acatcattca
    11341 ataagatgga tacttttttc cagccctatt taatgatcgt ttgttcattt tgatagtggc
    11401 gatgaaagca ttcctccggt tga
//
LOCUS       DAYLZO010000090        11416 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.091, whole genome shotgun sequence.
ACCESSION   DAYLZO010000090 DAYLZO010000000
VERSION     DAYLZO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.091"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..760
                     /locus_tag="ACGVJG_002205"
     CDS             <1..760
                     /locus_tag="ACGVJG_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFJ6558126.1"
                     /translation="LALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            970..1776
                     /locus_tag="ACGVJG_002206"
     CDS             970..1776
                     /locus_tag="ACGVJG_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558127.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1788..3008
                     /locus_tag="ACGVJG_002207"
     CDS             1788..3008
                     /locus_tag="ACGVJG_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558128.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2998..4584
                     /locus_tag="ACGVJG_002208"
     CDS             2998..4584
                     /locus_tag="ACGVJG_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFJ6558129.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            4623..6761
                     /locus_tag="ACGVJG_002209"
     CDS             4623..6761
                     /locus_tag="ACGVJG_002209"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFJ6558130.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(7129..8112)
                     /locus_tag="ACGVJG_002210"
     CDS             complement(7129..8112)
                     /locus_tag="ACGVJG_002210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFJ6558131.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            8490..9881
                     /locus_tag="ACGVJG_002211"
     CDS             8490..9881
                     /locus_tag="ACGVJG_002211"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFJ6558132.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            9897..10937
                     /locus_tag="ACGVJG_002212"
     CDS             9897..10937
                     /locus_tag="ACGVJG_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFJ6558133.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            10956..>11416
                     /locus_tag="ACGVJG_002213"
     CDS             10956..>11416
                     /locus_tag="ACGVJG_002213"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFJ6558134.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAG"
BASE COUNT         3783 a         1863 c         2263 g         3507 t
ORIGIN      
        1 tcttgcttta ttgatcagtg gatttgccag ttcttttgaa cgatggtatg tacgaatcat
       61 cggtgcagta gcagttgttg cagcttttct cgggatttgg gtctgccaat ctagaggtgc
      121 attagtcgcg ttgattgttt ttgcgatatt ggatctttta cctaaatcgt tgatgcgtgt
      181 cattcgtgcg ccatttattg cttacacggt tacgattctt ttagctttgc cgatttctta
      241 tctggcggct gtctctgaaa aagtcaattt gtttaccggt cgagaagata tttggcataa
      301 attctatcag acgctaggag aaaaatctga acaaatttta ctaggaatga aaacatttat
      361 cttccaacga ggaaaccaat ttttaggaaa ccacaatagt tataattcta ttttgaatat
      421 ctatggattg ataggatttg ggattgctgc tttgctgctt atccttttta ttggtcgctt
      481 aacgttgaaa gcagatttat ccaacgggca gatgactttt atctgggcct tttttgcggt
      541 gatgatgcag tcatttatgg aggatacatt gacttcatac ccatgggttc cgattattta
      601 tatcctgttg gctatggccg ttcatcgata tgatgaacca gaagttgcgg aggaattcta
      661 tgttatggaa gaggcatatg acgatatgac ttatgatgat acatcttatg aggaacaacc
      721 agatagcgct cattcttcca ggatccaacg ctatcattga taggcacaaa atacgagaga
      781 ggctgtgaca accgtcgcag ccttttttgg tttattgcaa ttgtctagca tcttctgtaa
      841 aatagaaacg ttagagaaaa tcttgtatca tagaaaatta agaaagaaat aagaacgcag
      901 ctcggtacca aaaatatggt aaaatggcac ttgactgtaa ttcactgaaa atcgcttgga
      961 ggaacacaaa tgtttaaaga tttgtattta tttatgaagg acatttatca gaacagaaaa
     1021 ttattgctac agttttcgat caatgacttc aaatcaagat atgcaggatc gtttttagga
     1081 atcttatggg cgtttatcaa tccgctattt actgtattga tttattggct ggtatttggt
     1141 gttggactga aagcacaaat gatcgatgca aaatacccat ttatcgtata tttagttacc
     1201 gggatgattc cctggttctt cttctcagat gcatttgcat caactacggt tgtttttcgg
     1261 gaatatactt atttagtaaa gaaagtcgta ttcaatattc gtatcttgcc cacgacgaaa
     1321 atcctatcca atctgtacac ccacttattc ttcattctga ttggcatagc tgtcgcgctt
     1381 ataaatggta tatatccttc agcaatgtct ttacagctga tttattattt attttgcatg
     1441 tgtgctttct tgacaggatt gacttggctg acggcatcga tccaaccatt tttacctgat
     1501 atcatgcaat taattacgat cgtgctgcag ttgatcatgt ggacattacc gatattatgg
     1561 agccctaatc agtttgattc aggtcttgta agcatattga aattaaatcc gctgtactat
     1621 gttgttcaag gttatcgtga gtcattttta agtgaagctt ggttctggga gcatggaaca
     1681 tatactttgt atttctgggt tttcacgtta gtaatgttcg taatcggctc tgcagtgttc
     1741 cgtagattga aaccacattt ttcagatgtg ctgtaaggag aatagctatg acagaatatg
     1801 caattagact aaaagatatc acaaaatctt ataatatgta cgcaaaacct tcggatcgat
     1861 ttcgtgaagc cctcaatcct tttaagaaat cctatcatga tgtattttat gcgttgaaaa
     1921 acgtgaatgt agaaataaaa aaaggtgaga tgatcggttt cgtgggagaa aacggatcgg
     1981 gaaaatctac aatgttgaaa atcattacgg gagtactgac accaacatcc ggcacgatgg
     2041 aaatcgaagg aaaaatctca gctttgctag aactaggatc aggattcaat cctgagtatt
     2101 ctggctatga aaatatctat ttaaatggaa tggttctagg attttcaaga gaagaagtcg
     2161 atgaaatggt cgatgatatc attgaatttg ctgacatcgg agaccatatt tatcaacctg
     2221 taaaaactta ttcaagcggg atgttcgttc gtctagcttt tgctgtagca atcaatgtga
     2281 atcccgatat tttgattgtg gatgaagcat tggcagtagg cgatcttgaa tttcaactaa
     2341 aatgtatgga aaaatttaca gaaatcaaga actcgggaaa gacgatttta ttcgtttctc
     2401 atgatatcag ttcgattcgt cgtttttgtg atcgttccta ttggttgaaa aacggagaag
     2461 tcgtcgaata tggcgatacg atggaagtaa cgacgaatta tgaaaacttt ttaaagaaaa
     2521 aatcagttaa aacagtggat cggaataaaa accaagtaga acggttcgcc gctccagata
     2581 tcgtcgacgt gacgaaagcg gaattgttgg ataaagacaa aaatccggtg gatatgctgg
     2641 aacaaggaga aaaactatac gttaaagtaa cttatgaagt aaaagatgat acaatcaaaa
     2701 atccggtttt aggaattgcg ctacgaacag tcgacaattt ttatgtatgc ggacttaata
     2761 ctctactaga tggtatcaag attccttgga aaaaaggaga gaatatcttt tatctggagt
     2821 atgaaaagtt aggtttgtta gctggtgaat attattttga cgttgcggtc tttgaagaaa
     2881 atgcaacggt tcctttggta tataagacaa agtacatgaa cttgtttgtc agcggttctt
     2941 atatcggaga aggcatcgtg gtcttagacc ataaatggga agaagggact catagcaatg
     3001 aaatatgatt ttgaaatgga cctagatgaa caaagcagtg tgggaaaaat tgccgcacag
     3061 atcaaaccag gaagtaaagt tttagagttt ggtcccggaa acggccgatt aacgaaacat
     3121 ttaatcggag caaaacaatg tgaagtgagc attgttgagt tagataagga attgttcgat
     3181 tttgtcagcg aatttgctca agatggattt tatggtgata tcgaaagttt tgagtgggca
     3241 aactattatg ctggacagac atttgattac gtcttgtttg cggatgtttt agaacatttg
     3301 gtagatcctg gaaaaacatt gaagaaagtc agagaatttt tgaatgaaga aggagagatc
     3361 ctgattacat tccctaactt ggcgcataat tctgtcatga tcgatttgtt caataaccaa
     3421 ttgccgtggg cttcttatgg tctcctagat gaaacccata attcttttta tactcatgat
     3481 ggcttccaaa aagtttttga aaaagcggga ttattcatta atattgaaga ttatttgtac
     3541 ttagctgtag gtgatacaga attaaaatca acttatgaag aattgccgga agctgtacgc
     3601 tatgatttca aaatgcgacc atttggtgaa gtctaccaat actttttctc attgatgaaa
     3661 catccagtag cacaaagttc cattgcagag ccacaaaatt ctaattatgt aaaagtgtta
     3721 gaagtaaccc agcaaacgaa acaagatgaa acaatccaac aaataccatt caataatttc
     3781 acaggggaaa atgagacact aagtttcccc gtatcagaaa caacagaaag aatggtgttt
     3841 cgcttcgcgc aacaacctag ctttatcgaa ttcagcgcag aagctgccgg agaaaaacta
     3901 acatttatcg attcgaatgc tgtagtcaaa acagtaaatg attgttattt gtttgatggg
     3961 aaagaattac cagagttcgt tctaacggat atttctggga aagaagtgac tatccattgt
     4021 cactatcgct ttatcgggga attgacacca acaatgaaag aattgttgga aacgattcgt
     4081 cccatggctg aagtcacaaa gcagctttct gagaaaaatg acgaattaga aagagaaaat
     4141 catcatcttc ttgaggaaaa tacaagacta gaccatacgt taaaaacaac cacaaatcgc
     4201 tattgcacat tgttggaaag tgatgaatgg acaataaaac atagactagg acgacgtaag
     4261 aaagaaactt cgaaaaaaat ccaagaaaaa gaactttcta tatgtattga cgagaaaatc
     4321 tgggatgcag aaacaaaaat attaaaaatc atcggatggg gaatcgctaa ttcggatcgc
     4381 cagcccttgt cctataagtt atcagctgac caatcgccat ttttcgaagc aattcctgtt
     4441 tcacgagaag aagtcaatca agcagaacag ctcgcaaaag gaacagaagc gggatttgag
     4501 ttacgaatcc tttgcgagca agaacgttca ttcttagtgg aagcagtagc tcaaaatggc
     4561 caatcatgga tcattgaaat gtaaataatg attaacttat aaagataagg agtaaacgaa
     4621 tcgtgcaaaa aaaagaaatt gccgtattta tcgatcaaat cactcgtgaa cgggccactg
     4681 gcgatttgct tattgtgggc tgggcaattg atgaagtgac caaagaaatc cctacgataa
     4741 aggtagaaaa agaaaatgtg attgctgaag caacacatgt cgtaagattg gatatcaatc
     4801 atttatataa tcttgatgtt aagacccaaa gcggttttaa ggtacgttta tcagggaaaa
     4861 tgagaggaaa agccatctta gacttccaaa cagcgagaca tcaaaatggc atagctgtga
     4921 aattaaacgg taaataccct tatgatgacg gcattgaatc ttcatgggaa agaaaaaaaa
     4981 gattactgaa aaaagggata aattatgcaa ggactcatgg cgtaaaaaaa gcgatccgtc
     5041 gggtcaaatt ggaattgaag ccaggatcga tcgactatgc agagtggatc agtcgtcatg
     5101 agaaaccaga tttgaaagag caaaggaaag aagcacaagg atttgcgtac cgtccattga
     5161 tttccatact tatgcctgtc tataacgttg aaatcaaatg gctcgaaaaa tgcattgatt
     5221 cggtactgga tcaaacgtat gatcattggg aactatgtat cagtgatgat gcttctactg
     5281 atccagctat ccgaaagtgt ctggaatcct atcaagcaaa agatgataga atcaaagtcg
     5341 ttttccgaaa agaaaatgga cacatcagtc tggcaactaa ttctgcattg gaaatagcag
     5401 aaggagaatt tatagcactc ttagataatg atgatgaatt accgccattt gcattatatg
     5461 aagtggcaaa agtgctaaac gtacatccag aactggatgt gatctacagt gacgaagata
     5521 aaatcgatgc agatggcaat cgctttgatc cgcatttcaa agcagactgg tcgcctgata
     5581 cattgatggg aaataattat atctctcact taggcgtata tcgaacaagc attgttaaag
     5641 aacttggcgg tttccgtaaa ggctatgaag gttctcaaga ttatgatttg gtccttcgtg
     5701 tgacagaaca aatccctgca gatcatattt accacattga ccgagtcttg tatcattggc
     5761 ggacgatccc aggatcaact gcaagtaatg gcgaagccaa aagttatatc tatgattccg
     5821 gagtgaaagc attgacagat gccctgtcta gaagaaacat caaaggaagc gtacgtcctg
     5881 gcagaatttc tggattttat gagattgctt atgacgtctt gcaagaggat ttggtcagtg
     5941 tgatcattcc aacgaagaat ggctatgaag atctaaaaac ttgtgtggat tcgatcatcg
     6001 aaaaaacgtc ttattcaaat tatgaaatca ttattgcgga taacggaagt acagatccaa
     6061 aaatgcaaga attatttgca gaatacaagc atcagctgaa agaccgcttt atcgtagaat
     6121 tgattgacat tccattcaat tattctcgaa ttaacaattt agcagcagaa aaagcaaacg
     6181 gcaaatactt tttattcttg aataacgata cagaagtgat tgagccagat tggatgacgg
     6241 tgatggtttc ttatgcacag tttgatcgaa tcggctgtgt aggagcaaaa ctgttttatc
     6301 cagatgatac aacgcagcat gcaggagtat tattaggaat cggtggtgtt gcagggcatg
     6361 cattgaataa ctatgatcga acacattgtg gttatttcgg ccgattggtc attgacgtca
     6421 attacttagc agtcactgca gcttgtatga tggtcaaggc agctgacttc aatgctgtta
     6481 acgggtttga tgaaacatta gaagtagcat tcaacgatgt tgacttgtgc ttgaaagttt
     6541 atgaactggg tcgttacaat gtctatgcac atcaagcaga attgtaccat ttcgaatcta
     6601 aatcaagagg ttatgaagat actccagaaa aacaaagacg ttttgctggt gaaatcaaaa
     6661 aaatgcaaga taaatggcca gcatatattg cgcatgatcc attttacaat gataatttaa
     6721 caaaagaagg aatcggcgac ttttcgttga gaccagatta aactataata aaaagctgtt
     6781 atgttgtgat tcgctcgcac ataacggctt tttatagttt tttgtcaaaa aaaggcagaa
     6841 ggtgccgact caaaaaaata agtcgaggtt gggacagaag tgtttaactc cgagaaataa
     6901 gaagaaattc acgaaaattg cttttcaaat ttttgtgaat ttcggcttat ttaagaagaa
     6961 gttgcttctg ctcccaccgt ttattcgttt ttaaagaatg agacaaaagt gtttcttact
     7021 tttgtcccac gctcttattc atgctcagag cgccaataga tacaaaatca tagccaaagt
     7081 ttcacgaacc ctagctgtca aagaatcgat attttatttt aatataggct attttaaaat
     7141 aaatggttta aggtattcct ctacaaaagc cactctatca attatcatgg gtttaaaata
     7201 agaagtgatg gagataacca gattgttctt agcgtcaata taaagaacat ttccactatt
     7261 accaattgct gaatatattt cgttctcaga atctaatacc caccataaat agccatatct
     7321 catattttgt aagtcttcta aagcactggt tctaggtttg aacatctcgt ttatccacat
     7381 gggtggtacg atttgttttc ctttatacat ccctttattt aggcataata caccaatttt
     7441 tgccagatca cttgccgata aacacaaacc atatcctgcc gtcccgatat ttagtggatc
     7501 acaaaaccag acattggttt taggagactt atccattgta aactgcttat gttcttcagc
     7561 tgtttcagca taataattga catggtttgg tatccctata ggatcaaata gatacttatt
     7621 ggcaaaatca acagttgtta gcttacttgt ctgatacaac aagccagtta atatgtgtaa
     7681 acagacagtt tgatagttga attcgccggt tatacctttt cttcctccca ataaatctag
     7741 tgatgttttt gtccaatctt cacttgaaca gacttttgtc catggatcgc ctttacattt
     7801 gtagggtgct ttcattgtta gcaagtgctt gattgtaata gcgtaaatag ttttctcccc
     7861 gcgtttcact ttatactctg gaaaataatc taatacttta tcatcgatac ttttgattaa
     7921 tccttgctct aaagcaatgc caatcagcaa agatacaata cttttcgttg ctgacatgat
     7981 atgacaagta tcgtcttttt tataaccatt ccattcatct gtataaactt ctaccccatc
     8041 cttatatgcg acaatttgac atatatttgg ttgtcgaaaa ccaataaaat catgcaactc
     8101 ttgtttattc ataagatatt acctcacttt cttctataag cataatgtca ttaaaaatcg
     8161 atttcaattt tttcgattct tcacgtaata caggtattct tatggactag acagcgcttt
     8221 gggaagatgg attctctgaa aaatataaag gttcacttta tgagtggata aaaaaagcag
     8281 caaatacttc gacttcttta atttaccaac attagtgaaa atcgattcat ctggagattt
     8341 gtaatttaga gtccttgtct tgattagagg tgtataaagc tttttattag acatttttaa
     8401 attccattac aatttgctta taaactaggt ttttaaaggt gactatggta agataataga
     8461 gatattattt tgtgattagg agtgtatata tgaataaaaa tggagaatgg agtgctgcac
     8521 gacggatatt cattatatta atggatgtta ttgtatacaa tgcagcaata tatagtagtt
     8581 tcttactgaa gtttcgtgga gagatcccat tacgaaattt agaaactttc caacactcag
     8641 caattattat ttcagtgatt tttattgcgt tgaacatttt gttgggtgca tatgtttttt
     8701 ataatcgaat gatcagtgac atcgtgtttg tcacagtcat cggtcaggta ttgatgacac
     8761 tagggatcat ggtagtgact tttgcgggcc gctggtttgc ttttccacgt cttgttatct
     8821 tactttcatt tgttctcggt acgcttttct taagtatcta tcgtattctc gtatataaac
     8881 tttatttacg cgtaagtcga gataaaaagg tcgtcattat tgggttagaa aaggatgtcg
     8941 caccagcaat ccaaaatttt ggttcaaaga aaaacaacaa acataaagtc gaagcagtca
     9001 ttcttgataa ctattatgca aacatcaaaa atatgattaa agacatcgat attgtctatc
     9061 ttgcttctca tatcgaagaa actgaaaaaa tcaaaatcta tcaattagtt actaaaaatg
     9121 aaaaaaaatt gtttttgaat acaacatttg aaaacttgac attgatcaat ccaaatatta
     9181 tgaatttcga agatgaaagt attattgaag catctggatt tagaatccaa cctgaatatg
     9241 aactattcaa acgtttgttt gatatcatcg tttcacttgt tttattagtt attgcttcac
     9301 catttatgtt attcacagca attttagtca aagtgacatc accgggacca atcatctata
     9361 aacaagtgcg gatcacaaaa aatcaaaagg aattttcaat ctataaattc cgctcgatga
     9421 ccgccacagc cgaagcaaaa tctggaccag ttttagctaa gagcaatgat gcacgagtaa
     9481 cgcctgtagg taaatttatt cgagctgtcc gctttgatga attaccacaa atcttcaatg
     9541 tgctgaaagg cgatatgtcg atcgtaggac cacgtcctga acgtcctttc ttcgtcgatc
     9601 agttcaatgc agaaaaccct tattactact tacggcataa tgttcgtgct ggtatcactg
     9661 ggtatgcaca agtctatgga aaatatgtct ctgattacaa cagtaaatta cgttttgatt
     9721 tactttatat caaaaaatat tcattgatgc ttgatttaaa gatcttgctg caaaccatca
     9781 agatcctatt tgataaaatg tcctctcaag gacttgaaga agacgaaaat gtgatctcat
     9841 tggcagaaat tgcttttccg aatgaaaaaa tctttcgcta gttgggaggc gtaaaaatga
     9901 acaatccgaa attcagcata atcgtaccag tatacaatgc agaagcaact attggtcgaa
     9961 cgattgatat tctaaaagaa ttggattatg aagagtacga agtcgtttta gtaaatgatg
    10021 gatcaacaga tcaaacacag gaaatcattc aagaactgat tgaaaatgat gaacgttttg
    10081 aattgattac tacgaccaat caagggcccg gattagcaag aaacgaagga ataagacagt
    10141 caaaaggaga atatctctta ttcttcgatg cagatgacac gccagtaaaa acgatcttgt
    10201 cagattatga tcatttgcta gaaaagagtc caaaagcgga tcttgttgtt agttcgtttg
    10261 tttttcgtac aatggacgag ggaagaatcg tttccgaaaa agaaaactta gttacagaat
    10321 gtcactatct aactcacgaa gagtttataa aagacatgta tcagttaatg aatgaacagt
    10381 tgatgtatgt tgtatggaac aaatgctatc gtagcgatat tctgaaaaat aaccaaattc
    10441 tatttaaaac ttataatagt tgtgaggatc gaatatttaa cttacattat tatgaatact
    10501 gtcatcaagt gatcatgaac ccaaaagtag aatatattta tgaatttgaa ggtgggaagg
    10561 ggatcactaa tcaataccga cctaacaagt tcgaaacatt taaagaattc tattcattag
    10621 caaatactgt aacagacgat attaataaag acggtatggc ggcgcttctt ttaaaaggga
    10681 caacttcagt tattttttct atttatagta cctctgcaag aacttcaaaa gagaagaaag
    10741 acgaagcaaa gcagatattg agtgatccag tgatccgaga agccaagcaa attgctaaga
    10801 cggattcttc agcaaaaaaa ataacaaagt tgctttacaa tatgccagct ccactatttt
    10861 tatctgctgt aaaagttgga agcttcgttg aagtgaaaat gccagggtta atggctgctt
    10921 tgaagcgagt atattagtta atggaggaaa atataatgaa aagaattttg cattttcagg
    10981 gacgaatggg tttaggtgga gcagagtctt tcatgatgaa tttatatcgt aaaattgatc
    11041 gtacaaacta tcaatttgat tttttgattt atgaagatta tgcagatgtt caagactatc
    11101 attcagagat tgaaagatta ggaggccgta tttttgttgt acctaatcct aagaaaaata
    11161 tttttaaata tttaatagaa gtgaataagt tactaaaaaa agaatctttt tctatagttc
    11221 acaatcaggt ttattttggc gggggaatta atttatggtt agccaaaaag aatggaatca
    11281 gacagagaat tgctcatagc catgcaactg aagatggaaa atctcaaaat attgtaatga
    11341 atgtattgcg taaattttta accaaattgt tgttacaaaa tgcaacagac taccttgccg
    11401 ttagtcaaga agctgg
//
LOCUS       DAYLZO010000091        11306 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.092, whole genome shotgun sequence.
ACCESSION   DAYLZO010000091 DAYLZO010000000
VERSION     DAYLZO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.092"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..351
                     /locus_tag="ACGVJG_002214"
     CDS             <1..351
                     /locus_tag="ACGVJG_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFJ6558135.1"
                     /translation="GVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGG
                     GATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDR
                     DVINDDSKVIDVNA"
     gene            962..1330
                     /locus_tag="ACGVJG_002215"
     CDS             962..1330
                     /locus_tag="ACGVJG_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFJ6558136.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            1510..2451
                     /locus_tag="ACGVJG_002216"
     CDS             1510..2451
                     /locus_tag="ACGVJG_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFJ6558137.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            2520..3251
                     /locus_tag="ACGVJG_002217"
     CDS             2520..3251
                     /locus_tag="ACGVJG_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFJ6558138.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            3395..5575
                     /locus_tag="ACGVJG_002218"
     CDS             3395..5575
                     /locus_tag="ACGVJG_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFJ6558139.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            5595..6077
                     /locus_tag="ACGVJG_002219"
     CDS             5595..6077
                     /locus_tag="ACGVJG_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFJ6558140.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            6093..6854
                     /locus_tag="ACGVJG_002220"
     CDS             6093..6854
                     /locus_tag="ACGVJG_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFJ6558141.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6985..8319
                     /gene="murC"
                     /locus_tag="ACGVJG_002221"
     CDS             6985..8319
                     /gene="murC"
                     /locus_tag="ACGVJG_002221"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFJ6558142.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8626..9111
                     /locus_tag="ACGVJG_002222"
     CDS             8626..9111
                     /locus_tag="ACGVJG_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFJ6558143.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            9104..9787
                     /locus_tag="ACGVJG_002223"
     CDS             9104..9787
                     /locus_tag="ACGVJG_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFJ6558144.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9913..>11306
                     /locus_tag="ACGVJG_002224"
     CDS             9913..>11306
                     /locus_tag="ACGVJG_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HFJ6558145.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTP"
BASE COUNT         3733 a         1946 c         2488 g         3139 t
ORIGIN      
        1 ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt gaatgtcttt
       61 agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt tggtgtagct
      121 ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat gaacttgcca
      181 gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc acgtacagca
      241 gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat caaagattta
      301 gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata gtcaaaaaga
      361 ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa aatagtttct
      421 caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca ccgtgaataa
      481 gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc
      541 tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttaa
      601 gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt caaaaaatag
      661 cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga gaacaccgtt
      721 taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt ccatataaat
      781 acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa cttatttatt
      841 attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta gattttccca
      901 ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta ggaggaaaca
      961 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
     1021 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
     1081 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
     1141 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
     1201 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
     1261 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
     1321 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
     1381 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
     1441 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
     1501 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
     1561 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
     1621 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
     1681 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
     1741 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
     1801 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
     1861 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
     1921 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1981 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     2041 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     2101 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     2161 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     2221 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     2281 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     2341 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     2401 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     2461 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     2521 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     2581 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     2641 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     2701 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     2761 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     2821 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     2881 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     2941 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     3001 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     3061 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     3121 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     3181 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     3241 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     3301 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     3361 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     3421 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     3481 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     3541 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     3601 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     3661 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     3721 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     3781 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     3841 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     3901 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3961 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     4021 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     4081 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     4141 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     4201 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     4261 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     4321 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     4381 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     4441 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     4501 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     4561 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     4621 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     4681 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     4741 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     4801 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     4861 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     4921 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4981 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     5041 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     5101 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     5161 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     5221 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     5281 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     5341 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     5401 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     5461 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     5521 cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     5581 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     5641 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     5701 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     5761 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     5821 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     5881 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     5941 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     6001 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     6061 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     6121 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     6181 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     6241 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     6301 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     6361 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     6421 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     6481 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     6541 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     6601 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     6661 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     6721 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     6781 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     6841 tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     6901 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6961 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     7021 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     7081 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     7141 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     7201 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     7261 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     7321 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     7381 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     7441 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     7501 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     7561 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     7621 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     7681 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     7741 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     7801 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     7861 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     7921 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7981 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     8041 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     8101 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     8161 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     8221 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     8281 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     8341 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     8401 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     8461 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     8521 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     8581 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     8641 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     8701 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     8761 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     8821 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     8881 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     8941 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     9001 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     9061 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     9121 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     9181 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     9241 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     9301 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     9361 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     9421 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     9481 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     9541 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     9601 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     9661 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     9721 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     9781 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     9841 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     9901 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9961 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
    10021 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
    10081 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
    10141 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
    10201 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
    10261 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
    10321 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
    10381 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
    10441 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
    10501 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
    10561 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
    10621 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
    10681 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
    10741 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
    10801 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
    10861 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
    10921 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10981 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    11041 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    11101 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    11161 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    11221 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    11281 gctgcaatca atgcgttgac accaaa
//
LOCUS       DAYLZO010000092        11236 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.093, whole genome shotgun sequence.
ACCESSION   DAYLZO010000092 DAYLZO010000000
VERSION     DAYLZO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.093"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..214)
                     /locus_tag="ACGVJG_002225"
     CDS             complement(<1..214)
                     /locus_tag="ACGVJG_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HFJ6558146.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQP"
     gene            complement(629..1399)
                     /locus_tag="ACGVJG_002226"
     CDS             complement(629..1399)
                     /locus_tag="ACGVJG_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558147.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(1392..2327)
                     /locus_tag="ACGVJG_002227"
     CDS             complement(1392..2327)
                     /locus_tag="ACGVJG_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558148.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2643..4292)
                     /locus_tag="ACGVJG_002228"
     CDS             complement(2643..4292)
                     /locus_tag="ACGVJG_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFJ6558149.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(4409..7012)
                     /locus_tag="ACGVJG_002229"
     CDS             complement(4409..7012)
                     /locus_tag="ACGVJG_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFJ6558150.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(7024..8073)
                     /locus_tag="ACGVJG_002230"
     CDS             complement(7024..8073)
                     /locus_tag="ACGVJG_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFJ6558151.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(8099..8413)
                     /locus_tag="ACGVJG_002231"
     CDS             complement(8099..8413)
                     /locus_tag="ACGVJG_002231"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFJ6558152.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(8429..8875)
                     /locus_tag="ACGVJG_002232"
     CDS             complement(8429..8875)
                     /locus_tag="ACGVJG_002232"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFJ6558153.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(8876..10798)
                     /locus_tag="ACGVJG_002233"
     CDS             complement(8876..10798)
                     /locus_tag="ACGVJG_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFJ6558154.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(11010..>11236)
                     /locus_tag="ACGVJG_002234"
     CDS             complement(11010..>11236)
                     /locus_tag="ACGVJG_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6558155.1"
                     /translation="FIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYL
                     SKKLVEGMGGEMTARLDGGIFSISVKLRRV"
BASE COUNT         3364 a         2315 c         1759 g         3798 t
ORIGIN      
        1 tcggctgttc atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat
       61 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
      121 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
      181 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
      241 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
      301 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc
      361 ttatcacaat tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag
      421 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
      481 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
      541 agctcaacaa acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
      601 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
      661 aataccaagt aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg
      721 ctactggcac atcgattgtt ccaaggaatc cgtaacggaa accgaagatc atataaacga
      781 tcggattgac tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa
      841 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat
      901 aagactgagc aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca
      961 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
     1021 tgatcgttac gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc
     1081 aaatgatcac atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt
     1141 tttgagagaa gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa
     1201 tcaaaccagg cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac
     1261 cgatcatttt tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg
     1321 tttggaccca gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
     1381 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa
     1441 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
     1501 gtcagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
     1561 tgttcatctt caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct
     1621 aaatcaaaga taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc
     1681 tcgatcagtt cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct
     1741 aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc
     1801 cacatctcgc gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa
     1861 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
     1921 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
     1981 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
     2041 tggaccgttt caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt
     2101 gctcggacta gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta
     2161 ttgactaaag aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat
     2221 agcgcataga aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca
     2281 cttccataaa cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
     2341 ccttatagac atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt
     2401 tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg
     2461 acgaagtcag acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat
     2521 gtgcaacaaa aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     2581 gtataaacgg cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc
     2641 gcctatccca atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg
     2701 gtttctacta aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc
     2761 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
     2821 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
     2881 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
     2941 gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt
     3001 tctttggaag ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa
     3061 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
     3121 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
     3181 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
     3241 tgccagttga ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct
     3301 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc
     3361 aatattgcag cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt
     3421 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
     3481 atgttgacat attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa
     3541 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt
     3601 gtcggcagtt ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta
     3661 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac
     3721 ccaccgattg ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc
     3781 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
     3841 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
     3901 tggatttttt ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc
     3961 tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta
     4021 gaataggtac tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga
     4081 tcatcgattt cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag
     4141 ctttcttctt tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt
     4201 tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata
     4261 ataattaaaa cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc
     4321 tttagtatag cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa
     4381 aggtgttttt ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat
     4441 catagggagg aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa
     4501 gggaaaggat agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa
     4561 caaatccttg ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta
     4621 attcaatcaa tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt
     4681 tatctaacct gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta
     4741 accgtttttc tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa
     4801 atccaaaagt gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat
     4861 gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc
     4921 caagttcccc tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact
     4981 cttttaaacc atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct
     5041 tatcgaatat ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa
     5101 tgacatcagg ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt
     5161 tatctaaaac attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga
     5221 gtgtgccttc aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt
     5281 ccatttcggg tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt
     5341 aaacaactaa tgtttcttgt aaagaatctt tatatacttt agctgtttct aattttagag
     5401 atagttctgt atctccttct aatggtgaat aatcatacgt atcgccatca tttgctgaat
     5461 cgatcaaatg aacgaaatca agatattgtc tatctccaac tatcaaattc aattgcccat
     5521 ctttaaaaga aagtttatat ttattgttac ttatctcagt tttgtctatc tcgattgctg
     5581 tttctaattt ttcattattt ttctcaaatt caacaacagt gtatcctagt gctggcacat
     5641 ttacatttat ttttatgtca agttcataat aagctggttc atcaaaatat tcgaaacctg
     5701 tagcaatcat ccgataagca tttttccgtt ctggaaaata aacttctttt tcaatgatat
     5761 tatagggact acctttgaag tgtattttct ttgaatcagc tactacatgc acgattttcg
     5821 atccactata attctcagtc gttgtgttga aaacaaggac ttcattcgat tttagcttta
     5881 atgcatcagc catccgtttt acaataaggt tttcgattcc gtcagctatc tcattagctt
     5941 ctttgattcg atggaaaata tcttccgcaa cattatctga aacacagcca ccaatactat
     6001 catgagcctg gttttgcaaa atctttttcc ataaccgacg aagtaggcct ttactgatat
     6061 ggataccatt tctttctgca atcagcatga gcggctcaac tcgttttaga attttttgtt
     6121 ccaatttaaa attttctatt ttcatttttg ttcttactga gccaatagaa cgatgaacac
     6181 gagagtaaac cggctgacga agttcacctt tatattcagg taattctttt ttgtttctga
     6241 taacttctac ataagaagga taatctgaaa taatattgtg gaatttactg tatttattca
     6301 acttctctac gacttgatca aaatttaaaa tcattgactt ttgatctaca cccgaaggta
     6361 ctaaaatatc tttgtttttt ccttgcatat gaagcatttc aactgcttca tccaactttt
     6421 cttttgcaaa ttcaggaata tctttaattg cgtctaaggt catcattcca gtcatataac
     6481 caaatggaaa attcatggca taaacaaatt tatctcccaa tcctttccag ataaaataag
     6541 gagatggaac catcgtttca aagtttaatc ctcgccaaca catgaaatta tcgatttcta
     6601 cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta
     6661 ccatcattgg atctccatat ttaaggatcg tatcactgat tccaatcatt aagtttctta
     6721 aaattgattc agcatccact aataaggcat ctgtttgtgc ataccacggt cccacgaata
     6781 gttgtcctgt tttcatcagt cgttggataa ccttaagctt ttgcggattg atctccaaat
     6841 actcatcgac gattgaagtt tgtccatcta aacaaaagtt tactttaggg ttcctttcca
     6901 attcatctag aacttcagta aagacctgat cagacaatac gatggcatct tcatttgtaa
     6961 aataccattc tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc atatcagatt
     7021 ctcctacttt ccttcttcta ctgaatttac ttgttgttct tttttatact tgatggattt
     7081 tagtgcattg accattaacg ctgtaaccac tcccccaata acaattgata ctaaatataa
     7141 aggtatattc gtaacaaaag gaattgccat aaaggttgaa agcattgttt cttgagtaac
     7201 accaaaaaat ccatttagac cacatgctac tgcagtgccc accacgatgc ttggtattac
     7261 tcgtaaagga tctttcacgg caaacgggat cgtcccttca gtaatcccca caaggcccat
     7321 aatcatagct gtctttgcat tatctcgttc cgattcatca taaagttttt tgtttaaaat
     7381 tgttgctaaa aacattccaa atggcggaat agcaattcct aataacatgg ctgtactagg
     7441 tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt tattgatagg
     7501 tcctcccata tcaaacgcca tcattcctcc taaaatagcc gacatcaaag cagcatttgc
     7561 tcctgacatg ccttctagcc aattagtcag tcctgttgtc attgcttgaa taggcgtagc
     7621 tagtacatac cataatatga ttccaacaac taatgtagta acaaaaggaa tcagcataat
     7681 tgattttaga ctaataactt gcatcggtac ttttactttt ttcaatgcat tagctaaata
     7741 gccacaagct aatccagcaa ccattcctcc aataaatccc ataccaagat tttgacacat
     7801 catcccagtg ataaaagctg gtgctagacc gggacggtca gcaattgaat atgcgatata
     7861 ggctgaaatc atcggtacga taaactgtaa tcccgtctga ccaatcattc gcaggactcc
     7921 ccagaatgta tccgtctggt cccaaacacc tgtgccacca aaaatgactg caatagaagt
     7981 tagtaaccct gctgctacga caagcgggat catataggaa accccacttt ggaaatgatt
     8041 tagaatttct cttccttgtt tcttgaattc cattagtaac tcccccacga tatatttttt
     8101 atttgttaat taatgcaatt gcttcatcaa tcgtctcttc accatctttt actacctctg
     8161 aggtccctac tgttaaaatc ggtttaccgt caaagcgacc tcgttctgaa acatttgtat
     8221 ctactgcaaa tatgacaaca tctgcctcgg aaacatccga aattgaaagt ttgttttcta
     8281 tccccatagc cccctgtgtt tctactttaa tatctaaccc tttcttcttc gcaaactttt
     8341 ctaaattagc ttttgccata taagtatggg caatccctgc gatacatgct gttactgcta
     8401 ctatttttgt catatgaaat tcctcctttt aaaatgatag agcttttacc aattcttttt
     8461 tatttctcga atcttttatt cttttaacaa actcgtcatc catcaatctt ccagataatt
     8521 ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc ttttattgct aacaaaaaga
     8581 cgagatcaac tggttgatca tctaatgcgc cccattcaac agaccgcttt aacttggcta
     8641 cagccactgt tgattcaatt actgctaggc ttttgccatg aggaatggcg atatttttcc
     8701 caattcctgt ggtcatatgg ttttctctgt caaatacgtc tttttgaaat tgtttgtggt
     8761 cagacacaaa cccattagtc tctaacaatt tcgataattt ttcgactaca ctttttttat
     8821 cagatgcatt taaatctatc actatattat ttatattgat tttttcactg atcatttata
     8881 agccttcttt caatatataa actaattttt caaacgacga actcgttttt aaatttgcta
     8941 ataactcatg attcgaagcg atttcattga atgtttcata aattttattc aattcaggtt
     9001 ttactttttc attaagtgca aaaaagaaaa cagaatgaac aagtgtccca tcccaatcaa
     9061 ttccttctgg attaacgtag acacagatta ctggcttttt tataaaatct acttcgatat
     9121 gcggcatagc aaaatcattc atcgctgttg aagatagttt ttctcttttg attgctccct
     9181 ctagtatccc ttctttcgcg taattttctt ttaccaattt tttggtaatt atatttaaaa
     9241 cgttcacttg attatccttt atttggtggg taaaaaagca aaatcgttta tctaataaat
     9301 ttagaaaagc atcttctgtt tgttcattta actgtagata ctgttttact tttctgacat
     9361 ctgcccctaa catcaaagga ctaacaacaa taacaggaaa atttgtattt tcaatcggaa
     9421 tcgtgcttat aacaagttca tcagtgactt ttctatcttg tagttcattg atgcttagta
     9481 catcttctat ctcaaccata tctgcaaaac cgttatttat tctttgctct aataattttg
     9541 atgttccata tccgctacta caaactaata tcactttcgt tttgtcaggt ttgtaacgat
     9601 ccaaaagtga ttgaatatgg agtgttacgt aagcgatttc atcttcatta aaatgtatac
     9661 agaatctctc ttcgatttcc tctaataaat ctacacttat cataaatgac cttttaaaag
     9721 aatatttgat tttgtccgta taagggttat agatatttac tcccaatttg agtcttttaa
     9781 ctgctgcatt gagatgtaat actagattat tgatcaattc ttcatcagga taaaattcat
     9841 ttaatttaat tcttactaac tcttgtagct caacacccat atctaaatag gaaagcttcg
     9901 tttgatctaa gtcgtgattc atagcatttt tttcaatcga agtaatgtga atgtcaacat
     9961 aagcttgttc atctattggc acaataattg aaaatgcttt ttcaatcatt ttgattaaaa
    10021 taagtgtatt ctttgagatt tcttcttcag aattattact acgtgataag aaattgcttg
    10081 tttcaatcct ttctatcgca atagcaaggt ggatagctaa agtttgaaat tcataatctg
    10141 taaatttcaa ttgtgtctta ttcatgtatc gattaagcaa ttcggctact tttttcaata
    10201 tgtccttatt taagaaatta ttcgcttcac caatgaaatt gatattcaag tctaagagat
    10261 ctgttttact gttagatgct gtaaagccac cccaataata gttcacaact tctgacatca
    10321 accttctttt acttttttca tcagctttga tgcgcacgcc atttcgatct gaaactaagg
    10381 acatcccatg cttgtttagt ctttttctaa catctttcat tgtgctttct accgtcgacc
    10441 tgctcacgta taattcgtca gcaagatttt gaatctttag agaatcatta gtattcaata
    10501 atttagcaaa cacataagtt agacggtcct ccggagagtc tgcgatattg attttatgac
    10561 tggataataa atttcgaagt gtttcttttt ttccaataag cgaaacgcct tttttaggtt
    10621 gaaccaacat ttgtacatct ggcatttttt ggattatttc tttcaaaatt aaaatatctt
    10681 tgctaattgt tttcgtagat acatataatt gttctgataa ttctttgtaa gttgtgtgct
    10741 cattttcaat caaaaagtat agtattttca tttgccgttc gctaaattcg atgcccatta
    10801 tcttcacctc ctgaatacag cttaagtgta caattattat ctctattttc atacagaaga
    10861 aagttcttga ttaatgtaga agtttctaat attcttgatt atttcaaatc aataaaaaag
    10921 cctgcaatat ttatatcaca ggcttttttc tctattaaat atatataaga taatacattt
    10981 ttctactagt tctcactaat cttgtataat taaaccctcc gcaacttgac ggagatggag
    11041 aagatgcccc cgtctaatct agcggtcatc tctcctccca ttccttccac caattttttt
    11101 gacaggtaaa gcccgattcc gctagattcc gtatttgtgc ggctggtatt ttcggtataa
    11161 aaacgctgga agactttttc aacagaggtt tgcgttcctg gtttgatagg attggccact
    11221 gttaatcgaa tgaatg
//
LOCUS       DAYLZO010000093        10925 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.095, whole genome shotgun sequence.
ACCESSION   DAYLZO010000093 DAYLZO010000000
VERSION     DAYLZO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.095"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            107..1248
                     /locus_tag="ACGVJG_002235"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACGVJG_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFJ6558156.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACGVJG_002236"
     CDS             1483..1935
                     /locus_tag="ACGVJG_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558157.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACGVJG_002237"
     CDS             1949..2638
                     /locus_tag="ACGVJG_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558158.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACGVJG_002238"
     CDS             2763..5114
                     /locus_tag="ACGVJG_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFJ6558159.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACGVJG_002239"
     CDS             5194..5589
                     /locus_tag="ACGVJG_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558160.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACGVJG_002240"
     CDS             5914..6531
                     /locus_tag="ACGVJG_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFJ6558161.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACGVJG_002241"
     CDS             6572..6871
                     /locus_tag="ACGVJG_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFJ6558162.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACGVJG_002242"
     CDS             6905..7072
                     /locus_tag="ACGVJG_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFJ6558163.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..7587)
                     /locus_tag="ACGVJG_002243"
     CDS             complement(7117..7587)
                     /locus_tag="ACGVJG_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFJ6558164.1"
                     /translation="MEFREGAFDFQTSQQNPSLDRLGRDYEDIKNTVAFMKQKKVALK
                     ILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKG
                     RPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(7593..8273)
                     /locus_tag="ACGVJG_002244"
     CDS             complement(7593..8273)
                     /locus_tag="ACGVJG_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFJ6558165.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
     gene            complement(8366..8761)
                     /locus_tag="ACGVJG_002245"
     CDS             complement(8366..8761)
                     /locus_tag="ACGVJG_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558166.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(8751..8963)
                     /locus_tag="ACGVJG_002246"
     CDS             complement(8751..8963)
                     /locus_tag="ACGVJG_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6558167.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            10035..10637
                     /locus_tag="ACGVJG_002247"
     CDS             10035..10637
                     /locus_tag="ACGVJG_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFJ6558168.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
BASE COUNT         3735 a         1675 c         2047 g         3468 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgatggg ttctgttgcg aagtttgaaa
     7561 atcaaacgcg ccctctctga actccaaata tcttaggctg ttattcccat taataccttg
     7621 atttcagtag acaccgaaaa gccgaagagc gttccatttc ttcggttctt tttatatatt
     7681 cctcgaatgg tctccatgcc cttaatcgtg gaagaggctg tacggagact ttgataaaat
     7741 ttatttcgtc gtttaatagg tcgatggtct tgttctatta aattgttaag atacttcaca
     7801 gttcggtgct ctgtcttagt atataaaccc acactctgta actttctaaa ggcggagcca
     7861 agagaaggtg ctttatcggt cacaattgct ttcggctcac caaactgttt atggagtcgt
     7921 tttaagaaag cataggctgc ttgcgtatcc cgtttctttc gtaaccagat atctaaggtt
     7981 aagccgtccg catcaattgc acgataaaga taatgccaac gtcccttaat tttgatatag
     8041 gtttcgtcca ttttccatga atagaaggat tgtctatttt tcttcttcca aagataatag
     8101 aggactttgc tgtactcttg tacccaacga taaatcgtag tatgacaaac atttattcca
     8161 cgatcatata acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca
     8221 acagcgacaa taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg
     8281 tcctctctgt cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac
     8341 agaacctttg tatgaacctt tatttctaca tttttttctc aagcacacta ctgataatga
     8401 ttaaaataat cggctggatt gcaatgatac ttccgacaat aatagctgga atgatgtagt
     8461 ctaaacaaat aaatataact gttgccaccc ctaacagtat tgtgttaaac atatttgtta
     8521 tatttcccgc tttttctttg attatgatat tccgttcgtc cataacattg attttgtagt
     8581 ctgcatcatt tttaatcatt ttatcatgca aaatttgaac atgagaaacg ccaaaaacgg
     8641 cactgaataa aatagcaagt ccaatatcta ccattttagg aaaatctgta accaaaattg
     8701 tcacaatcaa aaacaacgaa acaatataac cgatatacca aatctgaccc ttatttttca
     8761 tcttcacttt cctcctcata gataaaaatt tcttcaatgc tcatttgaaa gtaccttgca
     8821 attttaaatg ccaatataat agagggatta taacgtccat tttctaaaga acctattgtt
     8881 tgacgagata cttcaagagc agaagctaaa tcttcttgtt taattcctct ttgttttcgt
     8941 aattcttcca aacgattttt cacaacatgc tcctttctaa aaaatggaaa gtttactttc
     9001 ctttcgtgct tttataatac tcctgcgagc acaaaatgtc aaggaagctt tccatataaa
     9061 ttttgatttt aatgttttag ttactgtttt tatgacacat gaacacactt attcttattt
     9121 cctcttatct gctatcaaca gaaaagaaaa agccctgtca agagccgatt agatcaagcc
     9181 agtcgagaaa cgatgttaga ccaaattgat tttgggtaat gaatgtttag aacttagtag
     9241 tcatccaaaa acgactactt tttttgttga atcgtccaag gacgattcga tgcttttgac
     9301 ttgtaagaac aggattgaat gaaaccatac tgttcgcata tttttagtgg atgaacaaac
     9361 aaaatacgag agattttttg ttcgttcatc catggtttta gaaaaaagag ggacgatttt
     9421 ggaagaagaa aatcgtctct tttttttctt ctttttgtat gacaaaaaga aagatctttt
     9481 gcccatttta tttttataaa atgggtaggt ggcgttcgcg taaagcaaat cgatacaatc
     9541 caatggtttt ggcgaatcag atttaccatt ctaggcttgt ttaatacaga catcgattgt
     9601 tattttgaga atttatatcc ttattggcaa tgaaatggat attctccata ccaatagaaa
     9661 tcttcaagat aattttcgga aatatataag agttaatcaa ttatgatcat atagtaggta
     9721 aaaattagga cagccttcaa ttatatggga aaccaatata atcaagtaat tattcgggta
     9781 acacaaatac tagagtactc atttcttata actgttttac acctattagg gatcagtatt
     9841 attaatagat ctagataaat taatacgtaa ttatataata atttatgttt gtgccattaa
     9901 acaatcttaa cagatatttc attttattta tgtaaaacaa cacgaataat tattgcttcg
     9961 taaagttata tgagtataag ttaatttact ggaggtagtg caatgctttt ttctgaaaga
    10021 tttggtttta aaaaatggag gatatcatac tgagagatag attgacagac cctcttaaaa
    10081 atagtttagc aaatgtgatt tatgatatat tacgagaggc tgataaagtt tatagtaatt
    10141 ataatggaaa agtctcatgg gtaatatatt cttttttaat taaagagttt tttaaagaac
    10201 agataaataa tagctatgag ttgggttatc attttactgt ttattatgaa gatattatca
    10261 agaggttaga agacttggag tggtatgctt attatgattt attagaggtt tttgcaagtg
    10321 ctaaaatctt ggataaaaag aaaagagagg aagtaaataa aattctggag tcggaaaatt
    10381 ctggttacag attaagcgaa agcaatgaat ttattccaat cacagatgat atttctatta
    10441 agtctataaa tgctgtatct gaatcaccat ttaattacgc aaaaaatcat atccataaat
    10501 ctcttacata tataagcgag aaggaaaatc aagattataa tactgtagtg agagaagcaa
    10561 ttagtgctgt cgaaagttgt cttatcgaat tttcaggatt accagctaat aaaaaaatac
    10621 tttgggaact gctataaaag caattaaaga caaatcgaat aacaatgtcg atcttacatt
    10681 tttaaaacca tttgagacaa tgtatggttt ggcgagtaat aatggaatac gacatgcaga
    10741 aaatgaaaaa acaattgtct cagatttacc tgatgcagtt ttggtgttaa caacatgttc
    10801 agccgtaatt aattatttgg gagtaaaaat agcatatgac tccaataaat catgaaaagt
    10861 taaaattcat ccaagagcta cattataact attaatttcc aactctttaa aagtttagta
    10921 attga
//
LOCUS       DAYLZO010000094        10882 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.096, whole genome shotgun sequence.
ACCESSION   DAYLZO010000094 DAYLZO010000000
VERSION     DAYLZO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.096"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..739
                     /gene="msr(C)"
                     /locus_tag="ACGVJG_002248"
     CDS             <1..739
                     /gene="msr(C)"
                     /locus_tag="ACGVJG_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFJ6558169.1"
                     /translation="QKDSVQKAIQKNAKTLERRLQKIGETTKPQQMKQIRFPVPKSLE
                     LHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGSGKTTLLEHIRKQGEG
                     ILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSLLNHLGFAQETLTKPL
                     CTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEKLMQIYPGTILFTSHD
                     SYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            786..1232
                     /locus_tag="ACGVJG_002249"
     CDS             786..1232
                     /locus_tag="ACGVJG_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFJ6558170.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(1431..1946)
                     /locus_tag="ACGVJG_002250"
     CDS             complement(1431..1946)
                     /locus_tag="ACGVJG_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFJ6558171.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2063..3265
                     /locus_tag="ACGVJG_002251"
     CDS             2063..3265
                     /locus_tag="ACGVJG_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFJ6558172.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            3880..4206
                     /locus_tag="ACGVJG_002252"
     CDS             3880..4206
                     /locus_tag="ACGVJG_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558173.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(4323..4667)
                     /locus_tag="ACGVJG_002253"
     CDS             complement(4323..4667)
                     /locus_tag="ACGVJG_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558174.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(4743..5990)
                     /locus_tag="ACGVJG_002254"
     CDS             complement(4743..5990)
                     /locus_tag="ACGVJG_002254"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFJ6558175.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(5987..6793)
                     /gene="proB"
                     /locus_tag="ACGVJG_002255"
     CDS             complement(5987..6793)
                     /gene="proB"
                     /locus_tag="ACGVJG_002255"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFJ6558176.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            7023..7550
                     /locus_tag="ACGVJG_002256"
     CDS             7023..7550
                     /locus_tag="ACGVJG_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFJ6558177.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            7613..8983
                     /gene="radA"
                     /locus_tag="ACGVJG_002257"
     CDS             7613..8983
                     /gene="radA"
                     /locus_tag="ACGVJG_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFJ6558178.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            9017..10174
                     /locus_tag="ACGVJG_002258"
     CDS             9017..10174
                     /locus_tag="ACGVJG_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFJ6558179.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            10344..>10882
                     /locus_tag="ACGVJG_002259"
     CDS             10344..>10882
                     /locus_tag="ACGVJG_002259"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFJ6558180.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESD"
BASE COUNT         3332 a         1992 c         2346 g         3212 t
ORIGIN      
        1 acaaaaagac agtgtacaaa aagccatcca aaagaatgcg aaaacattag agagaagact
       61 ccaaaaaata ggagaaacaa ccaagccgca gcagatgaaa caaatccgtt ttccagtacc
      121 aaaatctctt gaactccaca gccgctatcc catcatggga caaaatgtcc aattggaaag
      181 aagtgggaga acattactgg taaatggtga ttttcagttt tctttaggta aaaaaatcgc
      241 gattgtcggt gaaaatggtt caggtaagac aaccttatta gaacatatcc gcaaacaagg
      301 agaaggaatc cttctctctc cgaaagtaag ctttcaagta tatcagcaaa agggttatca
      361 aatgacatct gaagaatcca tcattcgttt tgtcatgaga caaacagagt tttcagaatc
      421 gcttgtccgt agtttgctga atcacttagg gtttgctcag gaaactttga cgaaaccgtt
      481 atgtacatta agtgggggag aagcgacccg tctgacgatt gctttgcttt ttactaagcc
      541 aagtaatgtg ttgctgttag atgaaccgac taattttatt gatatggcaa cgatcgaagc
      601 tttagagaag ctaatgcaaa tatatccggg aacgattttg tttacttctc atgattctta
      661 ctttgtcgag cgtacggctg atgaagttta tgaaataaaa gggcagaaaa taaaaaaagt
      721 acttacgaga aatttttaaa tgtgctacga tgattttcgt gacaaattta aaggagtggt
      781 acagattgcc tacaaataaa aaagaaggaa taatttttac aacaatgatg tgttttttga
      841 tggtcttggg gatgagcgcg tataatttat ggctgcatca agatcttcat ttatcaagtt
      901 tgttgattgg tctagtgcct ggattcgtgg tggctttcat tttggatgtc ttcatcatcg
      961 gtgtgctggc gaaaaaaatt gcctttaaac taccagtaaa tccagaaagc aaggtccaaa
     1021 tgatcttaac tatttcatgc ttgatggttt taggaatggt gacatgtatg tctttattcg
     1081 gtgtgatcat ggaacaagga atacctgatc aattattctc tgcttatatg aatgcttgga
     1141 aaatgaatgt gattgccgct ttaccgttgc agttgatcat cgttggcccg ctttcaagaa
     1201 gtgtgctaaa agcaattcaa agtaatgaat gatggaacac tgttatttga aaaaaggcct
     1261 tggcatgctt tttgtcatag tctcttgaat gcagttctag aaaaatagtg atccgaacga
     1321 taggttttgg cctgtcgttc ggatcgcttt ttcttttcca actgtattgc tcttttattg
     1381 aagataattt gctcttcaat agatatttct agaaaggttc tgtttatccc ttactcaaaa
     1441 aactgctgca tttgttctct tacttgctga caccattgga gttcctgctg acaaagcata
     1501 attctgcgct ggatcacaac ataacgtcca aattcttgtt tcttgtctag cgagaggtca
     1561 ttcaattttt tttgattttt ttgcatgatt ttgttcagct gttgttctct agtgaatagc
     1621 agtccagtag ctgttgtttt atcgagcgtc gaaaaagcat agatttttgc tagaaattca
     1681 tctttttgcg taggagcggg agtttcctct ttgatccatt cctctacaag agcacgtcct
     1741 tcatcagtca agtggtagat ctttttttga gagtgctcgt ctttgtccaa aacggtcaca
     1801 taaccctgct ggtctaactt ggcaagcgtt gtatagatct gactgtgatg ggcttcccaa
     1861 aaaattgtga tcagctgctt caattcatac ccagaacgtg gtttggataa aagcacgcat
     1921 aataatatat aggctaaagt attcataaaa atcctacctc tcgtcttatt ttctttatta
     1981 tatcacagta aagtttccac gttttacatg tgcatattga catgtaaaat aaatcatgta
     2041 tgatgctaat agagagaaag ggatggaaat attggagaat caaatagata tacaagaaca
     2101 aacaaatgaa gaaacaaagg ggaaaagttc agcgctagtt gtagcagcta cctgtatgat
     2161 cgcttttatg ggagtgggtc tagtagaccc aatcttaaaa acgattgctg cacagttaaa
     2221 cgctacgcca gctgaaacga ccctgttatt tactagttac atgttagtca ctggtgtggt
     2281 gatgctattt acaggtttct tatctagtcg gatcggtttg aaaaaaacat tgatgacagg
     2341 attgtttatc atcgttgtat tcgctggtct tggcggatta tctgggaata tcggaatgct
     2401 gattgggtta cgtgccggct ggggtgtcgg gaatgcttta tttgtctcta ctgctttagc
     2461 aacgatcgtc agtattttag ttggtaacac agaaaaagca atcatgatgt atgaagcagc
     2521 acttggctgc ggaatggctg ttggccctct agtcggtggg gtcttgggca gtggttcatg
     2581 gcgttatcca ttttttgggg tagcattttt gatgttttgc ggttttatag ccctagcggt
     2641 acttttacct gaaactgaga aaccaaagag aaaagtaggc ctttgggaag gtgtcaaagc
     2701 gttaagcaat cataaacttc gttcagttgg actaatcgca ttgttatata atttcggatt
     2761 ctttacttta cttgcgtatg cgccattttt attgattggc tattctgaat tagaagtcgg
     2821 ttttgtattt tttggctggg gtgtgcttct ggctattgct tcgattttcg ttgcaccggc
     2881 tcttgagcgg aagtttacga cttatcggac aattttagca gcatttgtct tattcattat
     2941 ttgtttgctg ttattaggag ctggaacttc tatgccagtc cttgtaccga tcagcattgt
     3001 gttagcagga tttttccaag gaattatcaa tacactgttg acgacgattg ctatggaaat
     3061 accaggtctg caacgaaacg ttgcttcgtc cagctatagt tttgtccgct ttttcggtgg
     3121 agctttagca ccatttattg caggtgtgat tggtgaacat attggtgaaa gctatgcgtt
     3181 ttattttgca ggtgccattg ttcttatcag tgtatgttgt atcatttatc accgtaatta
     3241 ttttgctaca aatgaagaag aataagaaat cagaggttgt gaaaaagctg tcctgcatca
     3301 agtcataaga acagccaaac aaaaatagct tctcatattt ttcgtttggt tgggcttatt
     3361 accgcagatg caagcttttg aacaccgttt atcaggaggt tgtaaaatca gcgttttgtc
     3421 ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt ttttgagcta
     3481 ataccgaagg acagctgatt gaacaccgtt taacagaggt tgtaaaatca gcgttttatc
     3541 ctaagtatta gagaaacaaa acggaaaaat agcttctcat attttaccgt tttttgagct
     3601 aataccgagg gtcagctgat tgaacgccgt ttaccaagag gttgtgagac tgtcgtttaa
     3661 ctctgaatca caaggaacaa tttcaaaaaa cagtttctca tgtttgttga atattgtgac
     3721 ttatgattga ggagttggca gaagaacacc atttattcgg aaaaagggga tgtgacacga
     3781 tttttgtcac attccctttt tttctcaaaa taatgaaact ggaaaatttt cagtaggtaa
     3841 gctaaaattc atttaagaaa aagaatgggg ggataataga tgctggaaga atattttcag
     3901 gaagtcgggt tgacactcgt tgaattaccc attgaatttt cagatgatcg gttgatttat
     3961 cgtggctata tcaaagaatt gccaataatc aatgcggaag gtgaaactag aaaagcgatg
     4021 cagcaaaaaa tagctggtat gtatcaggtg tatcgggaac agctactggc tgaatcagcg
     4081 gaagaagaaa aagaaaaaac gatgaacttg tccatggatg agctgcttcg ttattatgat
     4141 ggcgaaacat ttgacggatt ttcgcttctt aacgaagagg aaacagattt accaggcagc
     4201 tggtaggcga acgccgttta tatggaaaaa gaggctgtga caaaatcttt gtcacagcct
     4261 ctaaaaagtc cggcagtttt ttgccgatct tcaaggcact ctacttatcg tacggatatt
     4321 ttttagcgga aagtccgatt caagtggttt ttattcagct gatatttata agataagtga
     4381 aaaatcccag caatcagtag cgtaatcgta taagcgattg aatgatcgaa aaaagaatag
     4441 taattgattg tcgctaataa gcataatgca accatgtaac taatgtgcaa gcgtgtaacc
     4501 agcccttttt gcccgatttt gtcaaaatac tgcgaccatt tttcccgttg actccctatg
     4561 cttcgttttt tgaattgaaa gaaaagggat aaaataccga ctcccagaaa agcagcgata
     4621 ttcagagaaa taaggtaaat aaaaaaaatc attaaaattt caaacatatt gacacctttc
     4681 tagttggata gaacctttac agaaaaaagg caaaaggcgg gaataatgtt tcacacgaaa
     4741 cattattcgc ggatttgtcc atcgccataa atgatatatt ttgtggaagt taattcattt
     4801 aaccccattg gaccacgggc atgcaacttt tgagtagaga taccaatctc tgcaccaaaa
     4861 ccaaataccg atccgtctgt aaaacgagtg gaggcattga catagacagc cgccgcgtcc
     4921 acttcattta agaaacgttg actagccgca taactatctg tcacgatcgt ttctgaatgt
     4981 ttagtattat gccgattgat atgtgcaatt gcttcatcca aagaatcgac cactttgaca
     5041 gcaaggatat aatctagata ttcagtatcc caatctgtgt cttctgctaa agcagccgtc
     5101 tctagatatt ctaacgcacg agcatccgca cgtaaagaga ccccgtgttc taccagttct
     5161 ttttcaatag ctggcaagaa aaaaggtgcg atctcagaat ggatcagcaa tgtttctgcc
     5221 gcgttacaga cagatggacg ggaagctttt gcattgacga tgatgttcac tgccatagat
     5281 aactgtgctt ctttatcgat atatacgtga ttatttcctg tacctgtttc aatgataggt
     5341 accgttgcct gttctttgac tcgttggatt aattttgctc cgcctcgagg aatcagcaca
     5401 tctaagtatt ctgtcaatcg catcaactgt cctgcagttt catgagaagt atcatcgaca
     5461 aactggatgg cataaggaga ttcgccagca ctgaccagta cttgctgcaa taattggact
     5521 aataattgat tcgagaagaa tgcttctttg ccaccgcgaa ggataacggc attccctgat
     5581 ttgaatgcta gactagctgc atctgtcgtg acatttggac gagattcgta aatgatgccg
     5641 atcacgccta gtggcacgcg ctttttgccg attctcaatc cgtcggtgtt cgtccacatc
     5701 tcatctactt ttccaatagg atcgtctaac gaagcaattt tttcgatctc tgtagccatg
     5761 gcattgattc gctcaggtgt cagctgcaag cggtcttgca tcgtttcact gatattgttt
     5821 tcgactgctt tttctaagtc tttatgattt gcttcaatga tcgcttgttg attctttttg
     5881 atggtttgtg ccatttgaac aagtagctca tttttgcggt ttgtaggcat aagtgctagt
     5941 tgttgcgcgc tatcttttgc ctgttgtcct aattgcatca agtcagtcat ttcaagtcct
     6001 ccttaaataa agtacctact tcttttcctg caagaatatc aaaaataatt gccggatttt
     6061 ttccatttgc taaaatcatt gcgctgtttg cttcaaatat cctagaagcg gcttttaatt
     6121 tactctgcat accgcctgtt ccaaatgtgc taccagcacc acctgcttgt gccagaatct
     6181 tttcatcgat tttatcgata tagcgataca tctgagcctc gggatttgta gtcggattat
     6241 ctgaataaaa cccgtcgata tcagaaagga cgatcaaaag gtctgccgcg acaagttttg
     6301 ccatgatggc tgacagctga tcattatcgc caaattttgt cagatgatct aattcatcca
     6361 ctgctacagt atcgttttcg ttcacgatag ggatcgtgtc cataatcaaa agctgttcca
     6421 atgtattcct cacattttta cgactttcag gataatctac cacgtctctt gtcacgagga
     6481 tctgtgccgt ctgttgatta tatacactga atctttgatt atagatattc atcagttcgg
     6541 cttgaccaac agccgccgca gcttgctgtt caggaatcaa agtcggacgt tctgacaaat
     6601 gtaatttatg cataccaacg cctacagctc ctgatgagac taagatcact tcctttcccc
     6661 gatttctaag atcagaaagg acaaaagcta actgatcgat tgccgcaaga ttgatatttc
     6721 cattgggata aatcaatgta cttgttccta ctttgacaat aattcgtttt gcatttttaa
     6781 tttctttacg catgtgacac ctcaatcaaa tatccactca ttaatttcgt tctattctac
     6841 ttgttttgac gagtaaagtc atgtaaaaaa tagttttttt gtacaacttg cagtcgtttg
     6901 gaaaaagaac gaattaatgg tttactttaa gatagcagaa tttcttgaag aatttgtaaa
     6961 gaaagtcttt tacagcctcg gatatgttag aataggagta gtttatttag ggaggaaaac
     7021 cggtgagaaa aagaggattt gaagttattt ctaaatataa agatcaaaat atcaatctac
     7081 cgcagcgcgc tacacatcat gctgctggct atgattttga agcagcagaa gaggtcgtga
     7141 ttccaagtat ctggaaagca ggctggaaaa aggggttgga aacagtcgca gatggattga
     7201 aaaaagatgt atcagaaaat gaaaaacaat tgaaaccaac cctcgtgcct actggaatca
     7261 aagcttacat gggagaagat gaatttttgc agcttgctaa ccgttcaagc aatccgttga
     7321 agcggttttt agtgttgaca aacggagtag gtgtgattga cagtgattat tacaataatg
     7381 aggcaaatga aggccatatc atgttccaat tcaccaactt tggtttcaag gatgtagtga
     7441 tcaaaaaagg cgagcgtatc ggccaaggga tctttttgcc gtttttaaaa gcagatcaag
     7501 atcaaacgac tgctcaacgt caaggcggat ttggttcttc cagtaaataa aacgttaaag
     7561 atacttaagc ttgatttaat gtctcatgat taggataaaa ggaggcggta caatggcaaa
     7621 aaaatcaaaa gtacaatttg tttgccaaaa ttgcgggtat gtgtcgccta agttcttggg
     7681 acgctgccca aattgcggaa aatggaatac gatggtcgaa gaaatcgaac aagatacaac
     7741 ggaccgcaga actcggacaa gtttgacagg agaaaaagca aaaccaacaa aaatcgcaga
     7801 cgtcgtccct aaaaaagaac ctcggatcaa aacaaaatta gaagaactga accgtgtgtt
     7861 aggcggaggt gtggtgccag ggtctatggt cttgattggt ggcgatccag gtatcgggaa
     7921 atccactttg ctgttacaag tttctcagca attagcagcc ataggcggaa aagtcttata
     7981 tgtatccggc gaagaaagcg cagagcagat aaaattacgc gcggaacgcc tcggaagtat
     8041 caacacagaa ttttatctat acgctgaaac agatatgaac gaaatcagtc gtgccatcga
     8101 acatatctcc cctgattatg ttatcattga ttccatccaa acgatgacgc agccagacat
     8161 cacaagtgtg gcaggaagtg tcagccaagt aagagaaacc actgcagaac tactcaaaac
     8221 cgctaaaaca aatgggatag ccatttttat cgtcggtcat gttaccaaag aaggctcgat
     8281 cgccggccca agaatgttag aacatatggt ggatacggtg ttatattttg aaggtgaaca
     8341 acatcatagt tttcggattt tgcgagcagt aaaaaaccga tttggctcaa cgaatgagat
     8401 tgggattttt gaaatgcatg agcatggatt ggaagaagtc gctaatcctt cacagatttt
     8461 cttagaagaa cgcctagacg gtgcaactgg ctctgctatc gttgtggcaa tggaagggac
     8521 acgtccaatt ttggtagaga tccaagcatt agtcacacct actatgttcg gaaatgccaa
     8581 acgaacaaca acaggtcttg atttcaatcg ggtatctctg atcatggcag tgctggaaaa
     8641 aagagctggg ttgcttcttc agaatcaaga tgcttactta aaagctgcag gtggggtaaa
     8701 attaaatgaa ccagcgattg atttagctat cgctgtcagt attgcttcaa gctataaaga
     8761 aaaaggaaca cagccaacgg aatgtttcat cggtgaaatt ggactgactg gagaaatcag
     8821 acgtgtcagt catattgaac aacgagtcaa agaagtccaa aaactcggct tcacaaaagt
     8881 atacctacct aaaaacaact taggtaactg ggaagcgcca aaaggaatcg agatagttgg
     8941 agtagcaact ttggctgaaa cgttgaagcg tgtgttccgc taactaataa atgattgtcg
     9001 aaacggagga aaaacgatgc aaaaacgtgt cattactttg ctgatgatca ttgtcggcgc
     9061 aagcttgggt atttcatttt taccctcagc ttggcaggca atcggtcaac agtcaaacgg
     9121 atggctgaat aatacattta ccaacagcct gttaggcgca cttattttct ttttattatc
     9181 tttgctgttg gccaaacata tctcagctat tatcaaacaa gtagaacaaa aactaagtga
     9241 ggtcagcttg acttatcttc tcttcggtgc gatcggtgcg atcatcggat tgactttagg
     9301 tgttgtgatc tcgacccctc tttacaatat ggaaattcca ttcgtcaata gtgtattgcc
     9361 gattttactg atgatcatct taggctatct gggattgaga atgggaacga cacgaatcga
     9421 agaatggaag aaagtttttg gttcacgaag caaaaaaatc gagcaagaaa cagctagtca
     9481 agttgagagt caactgttag aacgaaaatc aggagaaaat ttccataaat ataaaatctt
     9541 ggatacaagc gtgatcattg acggtcggat ttatgatatt acaaagactg gattcttaga
     9601 aggaaccttg atgatcccta attttgttct ttacgaacta caatacatcg cggactcagg
     9661 ggatagtttg aaacgtgtgc gtggtcgtcg cggattagat atcctaaatg ccttgcaaaa
     9721 agaagagggt atatctgtcg aaatgtacga tggagacttt gaagatatca gtgaagtaga
     9781 cagcaaattg atcaaattgg ctaagctttt agatggtgtc attgtaacca atgactataa
     9841 cttgaataaa gtctctgaat tccaaaatgt acctgttctt aatatcaatg cgttggcaaa
     9901 tgccgtaaaa ccagtcgtca tccctggtga aacgatgaat gttctagtag taaaagccgg
     9961 gacagaacgc caacaaggag tagcctacct tgatgacggc acgatggttg tcgtagaaga
    10021 tggacagcat tacatgaatg aacgtatcga agtcgttgta acaagtgccc ttcaaacagc
    10081 tgctggacgc atgatctttg ctaaacctgc acatgcagga cgaggaatcg acaataaaaa
    10141 agagacagag aaaaagacta aacaatcaaa ataaaaaaga aattcagcca actggtaagg
    10201 aaattcaacc agttggcttt actatttacc ttagaaaagg taaaatttgt gctgagtggc
    10261 tttaaataaa tgatatttga gaagccgtaa ctgacggaat ttttgctgtt agtcaaagtc
    10321 tagtttgaaa gaagaggaag aaaatgacga aagtacgcgt acgctatgca ccaagtccaa
    10381 ctgggcacct gcatattgga aatgcaagaa ctgcattatt caattatttg tttgcccgcc
    10441 acaatgatgg agactttatt attcgtattg aggatactga ccaaaaacgt aatatcgaag
    10501 atggcgaaaa aagccagttg gaaaaccttg cttggttggg aatgaattgg gatgaatctc
    10561 ctgaaaatcc tggagaatac ggcccttacc gtcaatctga acgtaaagaa atctatcaac
    10621 cgctgattga tcagctgctt gctagcaatc gagcatacaa atgctattgc acagaagaag
    10681 aattagaagc agaacgggaa gcacaacgcg caagaggaga aatgcctcat tacgcaggaa
    10741 catgtgcgaa cttgacacct gaagaacaag cagaaaaaga agcacaaggt ctagaatctg
    10801 ttgtacgttt ccgtgtgcct cgcaatacag aatacgcatt tacagatatg gtgaaaggcg
    10861 ctatttcgtt cgaatccgat aa
//
LOCUS       DAYLZO010000095        10857 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.097, whole genome shotgun sequence.
ACCESSION   DAYLZO010000095 DAYLZO010000000
VERSION     DAYLZO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.097"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..455
                     /locus_tag="ACGVJG_002260"
     CDS             <1..455
                     /locus_tag="ACGVJG_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HFJ6558181.1"
                     /translation="ISQTVIPEIDQPLENGYTKEEQKMINETRKILHQPALPVSATCV
                     RVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTSILARGTDMV
                     YVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            452..1813
                     /locus_tag="ACGVJG_002261"
     CDS             452..1813
                     /locus_tag="ACGVJG_002261"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFJ6558182.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            1773..2924
                     /locus_tag="ACGVJG_002262"
     CDS             1773..2924
                     /locus_tag="ACGVJG_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFJ6558183.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            2899..4068
                     /locus_tag="ACGVJG_002263"
     CDS             2899..4068
                     /locus_tag="ACGVJG_002263"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFJ6558184.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            4243..5622
                     /locus_tag="ACGVJG_002264"
     CDS             4243..5622
                     /locus_tag="ACGVJG_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFJ6558185.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            5903..6700
                     /locus_tag="ACGVJG_002265"
                     /pseudo
     CDS             5903..6700
                     /locus_tag="ACGVJG_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6889..7557
                     /locus_tag="ACGVJG_002266"
     CDS             6889..7557
                     /locus_tag="ACGVJG_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFJ6558186.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(7757..8077)
                     /locus_tag="ACGVJG_002267"
     CDS             complement(7757..8077)
                     /locus_tag="ACGVJG_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558187.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(8114..9496)
                     /locus_tag="ACGVJG_002268"
     CDS             complement(8114..9496)
                     /locus_tag="ACGVJG_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFJ6558188.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(9652..10839)
                     /locus_tag="ACGVJG_002269"
     CDS             complement(9652..10839)
                     /locus_tag="ACGVJG_002269"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFJ6558189.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
BASE COUNT         3412 a         2024 c         2243 g         3178 t
ORIGIN      
        1 atattagcca aacggtgatt ccggaaatcg atcagccttt ggaaaatggg tatacaaaag
       61 aagaacagaa aatgataaat gagacccgta agattctgca tcagcctgct ttgccagtat
      121 cagcaacctg cgtacgtgtg ccaatcgaaa atggacatgg agtctctgtg gcagttcaac
      181 tgcaaaaaga gtttacgacg gaagaaatta gagagtgtct cagccagttt ccagggattc
      241 aattagaaga tgatctagca gcacataaat atccgacgag cattcttgct agaggaacag
      301 atatggttta tgttgggagg atcagaaaag acacttcttt agagaacgga ttgttgttct
      361 atacgactgc cgataacata cgcaaaggag ccgctgcgaa tgccgtacag attgctgcag
      421 ctttaatgca gaaaatagcg gaggtggcaa gatgagtaat acgctgaaag tagcgaaatt
      481 cggcggaaca tcgatgtctc atgaagagca attcaaaaaa gtccgtacaa ttgttctttc
      541 agatcccaat cgccaagtga tcgttgtttc tgctttaggc aaacgtacgt cagatgatga
      601 caaagtaaca gacttacttt atttgattta tgcttacctt cagcatcatg ttgattggac
      661 acccctttgg aaaaggattt gtgatcgttt cattcaagta agagatgttc ttgccctgca
      721 gttacctatt gaagatgaac tagcacgcat acagaaacaa ctggaagacg aaaagataac
      781 tgaagattat ttagtaagta gaggtgaata tctgactgct caactgatgg ctgagtatct
      841 tggttaccag tttattgacg caaaagacct gattttcttt gactatgatg ggaaaatcga
      901 tcaggaaaaa acagcacagg aaatcacaaa agcatttaaa agatatccga aaatggttgt
      961 tccagggttt tacggggcga accctgcagg aaaagaaaaa ttattgggcc gtgggggaag
     1021 cgacattacc ggcgcgatac tggcgaagat gttgcaagcg gaaagttatg agaattggac
     1081 ggatgtttca ggaatcatga tggctgatcc gcgaatcatc gatcatccta aaatcatcga
     1141 ggaaatctct tttagagaac tgagagagat ggcttatatg ggggctaacg ttcttcatga
     1201 agcagctgtt ttcccagttc aagaggctga tatccccatc caaataaaaa ataccaatga
     1261 tcctttcgct caagggacaa aaatatcgaa tcacgaaata gaaaaggaaa atggactgac
     1321 aggaatagct ggacgcaagg attttctttc catcacttta ttcaaacgtc atatgtccga
     1381 tgaaatcggg tttatctgga aagcaatgag catttttgct aaacatgaga tatcaatcga
     1441 acatattcct tcagggatcg acaatatcgg agtgatcgtc tcagcagaag cgattgccga
     1501 taagttattt ctcatcacga aagaattaaa agaaacactt ggcgtggaag aaatcgtcgt
     1561 gatcgaagac ttagctttga tctctgtcgt tgggggtccg cataaagaat tgattggatt
     1621 atctgggaaa gtgttatcga tattgaacaa attagaaatt cgcacatcga tcttatcaca
     1681 aggagcacag gagttgaatc tgatcatcgg tgtaccgaat aatcaatatg aaacagtagt
     1741 gaagggtatc tatgaaggga tggtgaatac ggatgctgaa agaatatcga atggcactcc
     1801 atcaaattcc tgaaatcggg tttcatgaat tcaagacaaa aaaatattta tatgaacaag
     1861 taaaaaacag cgaggccgtt atccatgaaa tcggcgaaac aggactactc ctttattttg
     1921 ataatcagca agaagaaacg attgcttttc gaacagatat cgatgcactg ccagtcaaag
     1981 aagaaacgaa tctctctttt gcttctaagc atgaaggtta tatgcatgca tgtggtcatg
     2041 acggacatat ggcgatgctt ttagggttga ctgactttat aactaaacac attgaggatc
     2101 ttatttataa tgtagtactg atctatcagc catcagaaga aatttccggt ggcgcacaaa
     2161 gcgttattca gtcaggattg ttggataaat atgaagtaca agcaatattt ggctttcatc
     2221 tatggcctgg actagctaag ggagaagttt tctctcgacc aggacctttg atggcacaaa
     2281 gcagtgaaac agacattatc attaaaggcc agtctgcaca tattgcttct agtgaaaaag
     2341 gaatcgatag tctggaggca gctgtacgat ttttgaagaa tgtttatgat tttgaccggc
     2401 aattaccggc agagtggaat catctaatga aatttggcca aatccaaggt ggaacgatcc
     2461 gaaatgtttt agctggagaa gttattgtat ccggaagtat ccgttcttac agttcagata
     2521 tgcaaaatta tttgaagagg ggattagacc atttagcaga acagtttcac tctacagcta
     2581 ctatttcttt tcgctataat gacggctatc cagctgtaat caaccataga ggcttatatg
     2641 cagcactgtt agattcaaac aaactacatg aattagatgc tccagttctt caagccgaag
     2701 attttggcat atatacgcaa caatatccat gtgtattttt ctttttaggt gtcggtgata
     2761 cccctgcttt acatgatccg acgttcgatt ttgatatgga tgttttagaa aatggtgtag
     2821 aatggtacaa aacgattctt tttacagaaa atcttcttgt aaaaatcgat agcagttcaa
     2881 aagaggagcg agtaaataat gatgaattta tccaatcgat ttagtcctag gctttctaga
     2941 atagaagtat caaaaatacg acaattcgat cagcaaatat cgtcgatacc agatgtgatc
     3001 aaactcaccc tgggagaacc ggattttcca actcctgaac atgtcaagca agcaggaata
     3061 gcagcgattg aagaagactt ttctcactat accggaatga gaggattaga ggaactgcgg
     3121 gaagcggctt gtatatttca gcaacagcgt tatggattaa cttatgatcc gcaaacggaa
     3181 gttttgacga cagttggtgc aacggaagcg attgcctctg cgttgttatc tgttttggaa
     3241 gaaggagaca aggttcttat tccagcgcct gcttattcag gatatcagcc attagtggaa
     3301 ctagctggtg ctgaattgat cccgatagat acttctgaca cagggtttgt ctgtcagccg
     3361 gaacaatttg aacgagcatt tgaacaatat ggatcagctg tcaaagcagt cattttaaac
     3421 tatcctaaca atcctactgg gacgacattg tctgcaaaac aattgaaagc aatcgctgag
     3481 gttttaaaaa aatatccggt atttgttata agtgatgaag tatatgctga attgacttat
     3541 agtggaacgc atatgtccat agctacttat cttccagaac aaacaatcgt gataagcggg
     3601 ttatccaagt cacatgcgat gactggttgg cgagtgggct ttatttttgc tcaaaaagcc
     3661 cttattgacg aactaattaa agtccatcaa tatctggtga ctgccgctac aacgatgtcg
     3721 caaaaagctg cggctgaagc gctaataaat ggggtgaaag atagcgagaa tatgaaagag
     3781 caatatcaaa cacgaagaga ctatctggtc cagcaattgg cgcctttagg atttgaagtg
     3841 acccagccaa acggtgcatt ttacttgttt tgcaaactcc cagctgccat tcaaactgac
     3901 tcttggcagt tttgtgtaga attagcggaa caggcaaaag ttgcgtgtat cccaggaatt
     3961 gcatttggcc cagaaggaga aggttatatc cggatttctt atgctagcag catggaaaaa
     4021 ttacatgagg cctgcaaacg gatcaaagac tttttaacag aacagtgatt catagatttt
     4081 atcaaaaaaa caatcgttaa ttttgcggta tattctgcaa ggagacgatt gttttttaaa
     4141 tttagttcaa atgaacgcgt ttcttttgat tcataactaa tgtaatgtta agtttaaatt
     4201 gtttagaatg attttaaaag aatattcaag gaggtttcat caatggaaaa aatcaaagtt
     4261 gtcattgtag gtgcatctca tggagggcat caatcgatct tggaactatt gacacgatac
     4321 ggtgaaaatg tagatatcac gctatttgaa gcaggagact atatttcctt catgtcatgt
     4381 ggtatggagc tgtatttaga agacaaggta acagatgtaa atgatgtacg taatttcaga
     4441 ccagaaaact ttccgcaatc caatgttcat attttaaata atcacgaagt aaaaacgatc
     4501 gatgccgata aaaaaacagt aacggttact agcgcatcag ataacgaaac aaaagaatat
     4561 ccttatgaca aattgatttt gagttctggt gtcaaaccaa acaatctgcc agtaccagga
     4621 gcagatctag aaaatgtcta cttgatgcgt ggatacaatt gggcaaccaa aatcaaagaa
     4681 aaattaaatg atccggctat taaaaaaatc gctgtcgtag gtgcgggtta cattgggatc
     4741 gaagcagcag aagcaagccg aaaagctggg aaagaagtcg tattgcttga tgtgatcgat
     4801 agacctttag gcacttattt agatgctgag atgacagata ttttagaaca acacctaaat
     4861 gaaaacggta ttgaagtttt gacaaatgca aaaatcgaag cttttactgg taacggaaaa
     4921 gttgaggcaa tcaaaacaag tgaaaaagaa atcccagcag atctagtcat tcaagctgcc
     4981 ggtgtgaagg caaatacaga atggttaaaa gggatcgtcg acttagatga acgtggctgg
     5041 atccaaacaa atgaatactt gcaaacgaat cttccagatg tgtatgcagt aggagatgct
     5101 acacttgctt attctatccc agcacgcaaa aaaatgccga tcgctttagc gactgttgca
     5161 cgtagagaag cacgttatgt agtaaaacat ctttttgaag aagtaccaaa caaaccgttc
     5221 ggcggagtcg taggttcctc tgcattaagt gtctttgact atcattttgc agcaagcgga
     5281 ttgaatagct ttacagcagc aaaagcagga gtagatgtac gagtgtctta ttatgaagat
     5341 acgttacgac caaaatatgt accagaagaa caaggaaatc caaaagtgtc catccaatta
     5401 ttctcggatc caatgaccca tcaattgcta ggaggagcag tattatctac ctatgatgtt
     5461 acaggtcaag gaaatgtatt ggcactagcg atccaaaaca aactgacttt agaagaacta
     5521 gcagaagctg acttcttctt ccagccaggc tttgaccgac aatggagcat gttgaatcta
     5581 gccgctcaac aagcgttagg agaagaacca tttgtagaat aagaaaacga aatatttgaa
     5641 aagaggttgt gaaaaagctg ccttgcatca agtaataaga acagccaagt aaaaacagct
     5701 tcttatgttt ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt
     5761 attttcaaaa aagggactgt gacacgattt gtgtaccgac ccccaaaagt tagaccaaaa
     5821 aatctaactt ttggaggtcg gtatttttta tggttaaata tacttttgaa cttaaattaa
     5881 agatcgttca tgattatttg gatggcaaag gcgattggac cgtaaaaatc cagatcaaga
     5941 gattgaagcg gaaatgctta aaattcggga gaagcacaag gattatggct gtttacggat
     6001 gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa aagaaagttc agcgcttgat
     6061 caagaagcta ggaataaaag taacggcata cacgcgtaaa tcacgtcgct acaattctta
     6121 tcgaggaaaa gttggagcag tagccaaaaa tcggatccat cgtcgtttct acaccagtat
     6181 ctgccatcag aaaatcacga cggatacaac tgaatttaaa taccatgaag tggatgccaa
     6241 aggcattatc cgtcaaaaga aattgtatct agatcctttt atggatatgt tcaatagtga
     6301 gattttatcc tatcgtatct ctgaaaagcc caatgcatta gcggtaatgg aaggtttgga
     6361 ggaagctatc cagaccacta acgattgtcc ctatcgtcgt acgtttcact cagatcaagg
     6421 ttgggcgtat caaatgaaag cttatagaaa caaattgaag gagtacaaaa tctttcaaag
     6481 catgtctcgg aaagggaact gcttagataa ttcacttatg gagaattttt ttggcttatt
     6541 aaaacaagag atttttcacg ggaaagttta taactgtttc gaggaattaa aatcagccat
     6601 cgatagctat atctattact acaataatga acggataaaa cagaaattaa actggcaaag
     6661 tctcgtccaa tttcgtaaaa caaccgtaac ggttgtgtaa aaatatcatt ttagagcata
     6721 aaaaaaagga gtggatttct ccactccaaa aaagtctaac ttttgggggt cactacagtg
     6781 tcacagtcct ttttttagta aaaggataca tttcttcctt gtatattcca tgcgtgttcg
     6841 tgagaaagca tatataattt aattaacaga tagttagggg gagaaaaaat gattttttca
     6901 attacaatga aactggtgtt cgggttgatc ggtttattat tagtggtacg tttgcttggg
     6961 aagaaatcca tgtcagaaat cacaccgttt gatttagtct atacgcttgt tttaggtggg
     7021 atcttagaag aatcaaccta tgatgacaat gtccatgtag ggcatgtcct atttgccatt
     7081 gcgctttggg cagtaatgat ttatggtatc gaacgtatcg tccaaaaaaa tgaaaaggtc
     7141 aatcgttggg tcaaaggcga gccatctgtt ctaatcaaag acggcgtcat caacatgaca
     7201 gagttgacaa ataaccatat cgagatggag cagttgcgag caatcttaag acagcaagaa
     7261 tgcttttttc ttgaaaatgc caaacatgtt atcctagaga atgccggtca aatgagtgta
     7321 ttgaaaaaat cagatgaaga caaagcttta tcgatcctgc ttgtagatga agggcaaatt
     7381 cagcataaag tgcttcagtc caatcaattg actgaagcat ggcttatgga gaacttgaag
     7441 aaagaaggct atgcagatgt aaaacagctg atttatgttg aatggtcaga agaaaaaggg
     7501 ctctacatac taaataaaga agacgtcatg catgaatctt accgaataga tggatagata
     7561 gacagaggtt gtgaaatcag cgccttggct tgttactcaa aactaagcga ctttttcaca
     7621 acctctgata tctaatatac gttgttccag aagttgcttc ttcgaaaaat aagccaaaat
     7681 tccacaaaaa tttaagaagc aattttcgaa aaatcctttc ttattttttg aagttgatca
     7741 cttctgtcac aacctctcat gtcacaaacc atttttttgt ttcctttaac tcaatatacc
     7801 gtacgtcttc ccacaatatc ggctgatggt caatcataaa gtaatcttca taggcttcac
     7861 caatgaagtg cccttcgacc tcatccaaga gtcttccaaa ttcatctttt aaattgagct
     7921 gaatgattac tgctttacga gataaaaagg cttctgtcag tatactatcg atttcttgca
     7981 atgtcatttg ttgtttagga agaggctgtt tgcgtgcttc ttcttcattt ttttgatacc
     8041 gttgactagt tcttccatcg caaaagcagt cccccatttt aatccaaatg gccgatctcg
     8101 atactcgtta tagtcaatga atttttttgc tgtccgtctc atcttttcca ccttctaatc
     8161 cactcatacc acctgcatgc ccgccaacga gagaactacg tgcgatggcg cgtcctcctt
     8221 gcaagagact atgcgcatgg atgattgatt gaaagccgta tttttttcta atcgtatcga
     8281 caacaaaatc aactttcgct tcatttatct gcttttcagg atcttcaaaa aggttaagct
     8341 gtaaacctgt cgcgtatacg aggtcggaac agttcacgcc tacacgccga atatcctggt
     8401 agtggtaatg ctggtggaaa atggtcaata aagcatctgc tagatctctg ctagcatttg
     8461 tcgcttcgat tttcatttgc tggtgaaacc cggtcttgcc atttggatca agatagccta
     8521 aagagtatcc tacccataag ctgactactt gtgtttttgc tgcagatcgt ctcaagcgag
     8581 tgccgacttg gtcggccatt tctttgataa cgatctcgat ttcttctcgt cttgtataat
     8641 ctctatctag gatttgacta tttccgatac tttttgattt tggtgtatat tcttgtccca
     8701 aaaaacttcg atcgatgccc caagcatggg cataaagctg agtaccgatg acccctagct
     8761 tgcttttcaa gtgataataa tccgcgtgag ctaaatcata aacagaatgt atacccattt
     8821 gtttcagacg ttttgctgtt cgtttgccga ttccccaaaa gtcagtgata tctgagattt
     8881 tccataactt ttctggtaca tcttcataac gccattcagc aaacatttgc tttccgtctt
     8941 ttgagccatt gtctaaggct agtttagcaa gtagcgggtt atcgccaatt cctatagtcg
     9001 tgtagattcc ggtctgctca aacacatctt tatggatcag tttagccatg tcgtacgcat
     9061 ttgttttacc aaagagtgcc atgctatttg ttacatcaag gaagctttca tcgacggaat
     9121 agacatgatg gttcgcttcg tctgcatatt ttttatagat agaattgatt tctctgttct
     9181 tttccatata ataggccatc cttggggcaa caatatataa atcacttgga taggggaagg
     9241 gaagatctct ggcacggctg atattggtga tgccgtaagc tttttttgct gcaggactgg
     9301 aagccaagat taatccgcta ccccgtgttt ctgtcgaatc ggacggatag gacatgacta
     9361 ctaatttagt tgtaagggga tcaagatcct tttcaacgca ctctacggag gcgtaaaaag
     9421 atttgcagtc aatacataaa atatctcttg aaggttcttt ttcataaaca aataccggat
     9481 tttgattcgc ccacataaca atcacctctt caacacaatt agaacacacg ttctctttcc
     9541 gtgtcaataa aaaacagcaa ttttcaaata cgagcaaaag gctcacattt ggaaattgct
     9601 gagaggttat ttttatctat ttgtttttag ctgagatcgt tgattttttt atcaaacatg
     9661 aacagcttct ggatcgatct ttgttgtccc atttccaatg tgtgcaacat aaaattccgg
     9721 agcatagccg acaactttca tgtattcatc atagacattg tttttgaagg caggtatatt
     9781 ttcttctttg acaagagcaa tcgcacagcc accaaatcca gctcctgtca ttcgtgcacc
     9841 taagacacct tcttgttttt gagcagcatc tactagtgta tctaattcga tcccagttac
     9901 ttcataatca tagcgaagag agtggtgaga atcatttaac agttgaccaa atgcttctaa
     9961 gttcccagct tctaattcag cttttgcctt caaagtccgc tgattttcag aaacggcatg
    10021 gcgagctctg cgtatcaatg tatcatcgcc gatttggtca atagttgatt caaattcttc
    10081 ttcatctaag tctcctaaag aagagatagg caaagttttt tgcaaacgtg ccaaggcttc
    10141 ttcgcattcg ctacggcgtt cattgtattt tgaatctgcc aattcccgcc gcttattcgt
    10201 attcatgatg acgattgcat aaccatctag tttcactgga accatttcgt atttcagcgt
    10261 atttgtatct aataagattg catgatctag ctcaccgaag ccgatggcaa actgatccat
    10321 gatacctgaa ttgacaccga tgaattcatt ttctactttt ttacctgttt ggactagttc
    10381 caagcgatct gtttttaaat caaacagatc ttctaataca acacctacta ataattctaa
    10441 tgaagcgcta ctggaaagtc ctgcaccatt agggattgtt ccttccacta ataactcaaa
    10501 cccatgatcg attgagtgtc cagcttcttt taatttaagg accattcctt ttacgtaatt
    10561 tgcccagttt gcttttttat catattctag ctcatctaaa gtaaaactaa tgacaccgat
    10621 atcgtcaaag tttgtggaat aaactaatat tttattatcg tctcgaggtg ctgctacacc
    10681 ttgcgttcca taagtgatgg aagcggggaa gacatggcct ccattgtaat ctgtgtgttc
    10741 accaatcaaa ttgatgcgtc ctggtgagaa gtattgtgta gtttttttag gtccgaatgt
    10801 ttcggcaaat tttgtgttta agcttctttt gatttccatg atgagttatt cctttct
//
LOCUS       DAYLZO010000096        10659 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.098, whole genome shotgun sequence.
ACCESSION   DAYLZO010000096 DAYLZO010000000
VERSION     DAYLZO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.098"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..513)
                     /gene="dctP"
                     /locus_tag="ACGVJG_002270"
     CDS             complement(<1..513)
                     /gene="dctP"
                     /locus_tag="ACGVJG_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HFJ6558190.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGP"
     gene            complement(530..1636)
                     /locus_tag="ACGVJG_002271"
     CDS             complement(530..1636)
                     /locus_tag="ACGVJG_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFJ6558191.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(1865..4462)
                     /gene="adhE"
                     /locus_tag="ACGVJG_002272"
                     /gene_synonym="adhC"
     CDS             complement(1865..4462)
                     /gene="adhE"
                     /locus_tag="ACGVJG_002272"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFJ6558192.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(4767..5147)
                     /gene="yajC"
                     /locus_tag="ACGVJG_002273"
     CDS             complement(4767..5147)
                     /gene="yajC"
                     /locus_tag="ACGVJG_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFJ6558193.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(5244..6389)
                     /gene="tgt"
                     /locus_tag="ACGVJG_002274"
     CDS             complement(5244..6389)
                     /gene="tgt"
                     /locus_tag="ACGVJG_002274"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFJ6558194.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            6607..7305
                     /locus_tag="ACGVJG_002275"
     CDS             6607..7305
                     /locus_tag="ACGVJG_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFJ6558195.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(7473..10112)
                     /locus_tag="ACGVJG_002276"
     CDS             complement(7473..10112)
                     /locus_tag="ACGVJG_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFJ6558196.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         2937 a         2324 c         1920 g         3478 t
ORIGIN      
        1 cggaccgtct accatataaa agctgcgctg accagaatca taataggtca atccggtaaa
       61 tcctaaattt ttggtcgatt ggtagattgg ctccattatt tcttcattat ccatcacctt
      121 aaagaaatgt tcttcatcat tgaatagata aggaactgaa aaaatcgaat aatctttaga
      181 gaaactttcc aacgctgagg cacttacttt tgtgaaatct actgctcctg actgtgtcaa
      241 ttcgatcagt tcacgttctc cacccagttg tccatctggg aaatactgga tctgtacgga
      301 accatctgtt ttttctttta ctaaacgccc aaattcattc attgcatcaa ttactggctg
      361 gctgttgctt gcataagcaa agcgcaaggt aactatctca tttacatctg catctgcgac
      421 agttgccaca ctagaattat ttccgcagcc gcttagacct agcagaccta ggctaagcga
      481 acttgctgca aacagcagtg atgttttttt catatgggtt tcctttcttt taacaataag
      541 agacgagcaa ttctgtcaaa caaagcaccg ttaacgactg tccataaggc attgctgtga
      601 ttccgatatt ccgataaaaa tctaaactgt cccccattcc tgtcccgatt gaaacgtttt
      661 gaacttctcc tttttcatcg atttgttcaa gcaatccttg gatagcacga taagcgactt
      721 ctttatattc ttgtggtaaa taacgtttat ggaccgcttt caaaataccg taagcaaatc
      781 ctgcagttgc agaagattct acgtaagaag aaggatcatc caacaacgta tgccataaac
      841 cgctttcaga ctgataagtt tttaatgcac gcacttgtgc ttcaagtgtt tcgatcaata
      901 atttgcgcaa tgcatcttct ttcgataatt ctaagatttc aatgatctct ggaatagcaa
      961 tcgtcaacca gcagtttcca cgagcccaaa atgcttcggc atagttatga tttccttcaa
     1021 acgtccagcc gtgataccac aatcctgttc gtttatccat caagtatttg atatggatca
     1081 taaactggta acgagcttct tctaaatatt ctgggcgatt caacagtttc ccgatttttg
     1141 ccaaaggaag cactgtcatc atcaatgtat cgtcccacaa ctgattctta ttttccggtc
     1201 catatgtggc atgctgtaac ccgtcttcat ttgtccgagg catgtcattc atgacccatt
     1261 ctgcccattg ttctaaataa ggcaaatagc gtgaatcttt cgtgtcttca tacaagtaag
     1321 ccatagtcaa tagcggtgcc attgtattga cgtttttggg cggagcgcct tcttccatac
     1381 gatcctcaaa ccattcattg accacttgat aagcgcgagg attttttgtc atctggtaat
     1441 ttttataaat accatataga ccgacacctt gtggccaatt ccacactgtc cagcttttgt
     1501 catctacttt cagtccatca aagtccaaca aaaattcacc attatcgtct ttgatcgttg
     1561 tcaggttttc aacgagcagg tcaatttttt ctttcactaa ttctttaggt acaaaaatct
     1621 cttctttaat aatcatcttt acgccacctt tttaaacgct ttcattttat atccttaata
     1681 tagcagtaat accgtccact ataaatatcg ctttttgaac aaaaactgtc taaaattgac
     1741 ctaaatgtta aataacaaaa gtaactttca gttacttgga aattaaaaaa agaacttgga
     1801 gaaacaacgt tttccaagtt ctttttcatc tattttctta tcttttttct acgctttctt
     1861 ttgcttatcc gttgtaagca tcaatgatga tctgtttcaa ttcagagatc aatggttctt
     1921 tcggattagc tgtcgtacat tggtcttcgt aagctaattc agccatacgg tctacggttg
     1981 tatctagtgt ttcttgtgtg actccttgag cttttagact catgttgatg ccgacagatt
     2041 ttcctaattc ataaacagcc gttgctaaag cttcgactaa ttcctctgtt gtttcgcctt
     2101 tcagacctaa gaattttgcg atatctgcgt aatctgtatc tgcacggaaa tagtcatatt
     2161 tagggaacat cgcatgtttt tgtggatctt ttgcattata gcggataata tgcggtaaaa
     2221 gaatcgcatt tgtccgtcca tgagggatcc cgtattcccc gccgatttta tgtgcgactg
     2281 agtgacaaat acctaggaat gcgttagcaa acgccatacc agccattgta gaagcgttgt
     2341 gcattttttc acgtgattcc atatcaggtg tttttactga tttttctaaa tagtcaaaga
     2401 ctaacttgat tgcttgtagg ctcaatccac gtgtataatc tgaagccatg actgaaacat
     2461 aggattcgat tgcatgtgtt aacacatcca tcccggtatc tgctgtgaca gatgctggaa
     2521 ctgacatgac gaattgagga tcgacgattg cgacatctgg cgtcaatgca taatctgcta
     2581 acggatattt cacatgtgtt tcactgtcag tgatgaccgc aaatggcgtt acttcagaac
     2641 cagtccctga tgttgtcggg atacatacga attgtgtttt ttctggtttg tcgatcttat
     2701 atgtccgttt acggatatcc aagaattttt gttttgcacc aaagaaagat gtgtctgggt
     2761 gttcatagaa catccacatt cccttagctg cgtccattgc agaaccgcct ccaagagcga
     2821 ttactgtatc tggcttgaag tctgccatca gtttcgttcc tgcgtatacg gtatttgtcg
     2881 atgggttcgg ttcaacatct gagaagactt cgatcttcac atcattttta cgtcgtgcca
     2941 atacttcacg aactgtatcg cagtaaccga attggaccat accagggtca caaacgatca
     3001 tgacacgttc aacgttttcc attttctcta agtacaatag agagttcttt tcaaagaaga
     3061 tttttggcgg taatttgaac cattgcatgt tatttctccg tttcgctaca gttttgacat
     3121 tgattaagtt tacagcagag acgttacgag aaacagagtt cttaccataa gaaccgcatc
     3181 ccaaagtcaa tgacggaatc atttcgttat agatatcacc gatgccgcct tctgcagatg
     3241 gtgtattcac taaaatacga caagctttca ttcgaagacc gaatttgaca tgcaactctt
     3301 cgttttctga atggataact gctgtatgtc ctaatcctcc aagattcaac ataccttcac
     3361 acaaatcaaa cgcatggtcc gtgttgttag ctttgatcat ggcaagaact ggagaaagtt
     3421 tttctcttga tagcggataa tctgggccta cgccttccaa ttcagcaatc agcattttcg
     3481 ttccttttgg tacactgatt cctgccaatt ttgcgatttc catcgcagaa tgtccaacga
     3541 ttgatggatt gaccgcatat tttccttcgt tcatcactgc atcttctaat ttttgcaatt
     3601 catctggttt tacgatataa acttggtgtg cttggaattc tgcttttact gctgcataga
     3661 tttctttatc cacgatcaca gcttgttcag aagcacaaat cattccatta tcaaatgttt
     3721 tcgaaacgat caaatcgttc actgcacgtt taatttttgc tgttttttca atataagctg
     3781 gtacgttacc tggacctaca cctaaagctg gttttcctgt agagtaagca gacttgacca
     3841 ttccagctcc acctgtcgct aagacgatcg cgatacctgg atggttcatt agcatagaag
     3901 tcgcttcgat cgaaggatgt tcgatccatt ggatacaatt ttcaggagct ccggcagcga
     3961 tagcagcatc acgtaccact cgtgctgctt ctgcagaaga tttttgggca cttggatgga
     4021 aagcaaaaac gattggattt cttgttttca acgcgatcat tgatttgaaa atcgttgtgg
     4081 aagtcggatt cgttgttgga gttactccgc aaaccacacc gactggttca gcaatttcaa
     4141 tcaacccttt ttggacatct tcattgatca caccaactgt tttatcatgt ttgatattat
     4201 gccaaatata ttcggacgca tacatatttt tgattgcttt atcttcgtaa acaccgcggc
     4261 ctgtttcctc tacagccatc ttcgctaatg gcatatgttg atccaaagct gccattgcca
     4321 tttcatgcac gatatggtct actttttctt gatcaaagct ttccatttct ttcaatgctg
     4381 catttgcttt ttctgccaaa tcatcaatca ttgcttgtac gtcaattgtc ttcgtttttt
     4441 ctttttctat tactttagcc atcattatcc tcctaatggg tcatggttat ttgttaaata
     4501 attcacatgt atatcataca atatttattt ttctatgtaa acactttttt gtgatttttt
     4561 tcacagttat ttttaaatat aaaattgctt tatttacagg tgtttgtata gaaaaatata
     4621 taaagttatt tattattatt tatatgacac acccgattat tcaatattga gcaatatatc
     4681 ccatgctctt atctctttct tttttgaata aaaaatgagg taggacatct ttctgcccta
     4741 cctcgtcttt aaataagctt tgatacttat ttttcttcct tctcttctgt ctcttccagc
     4801 gtttctgttg tttcagtttt tacttcaact gtctcagctg gttcttctgt tgtttcaaca
     4861 gtagtcgttg catcatttgt tacgacagtc cctggtttga ccgttttgat tgctgcacga
     4921 tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg tccgtttgtc attgtcgatt
     4981 tctgaaacaa ctccatgtaa tccgccgatc gttacgactg aatcacctgg tttcatgcta
     5041 tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg aacgtgacat aaaaaaccac
     5101 attgctaata gcgcgataaa catcacaagc attggtaatg ttcccatttt tttcacctca
     5161 ctaattctgt atctacaaaa aacactttag cagaaaaatg ctgataacac tagtttttta
     5221 cttaattttt aaaagatttt cctttagaag ctttttgcgt tttctttatt gaacccgtat
     5281 tcttcaaaaa aggcttctct aaattcaagc aaattatcat ccatgattgc ttgacgtact
     5341 tgtttcatta gattcaacaa gaagtataaa ttgtggtaag aagtcaaacg aatcccaaat
     5401 gtttcatcac atttgatcaa atggcggata taggcacgtg tatagttttt acatgtataa
     5461 caatcacatt tctcatcaat tggacggaaa tcatgggcat atttcgcatt tttcactact
     5521 agacggcctt gagaagtcat acatgtgcca tttcgagcga ttcgagtagg caagacacag
     5581 tcaaacatgt ctactccacg gataacacca tcgatcaatg aatcagcagc accgacaccc
     5641 atcaagtaac gtggtttgtt ttcggggatc aacggtgtag tgaattctaa tacacgattc
     5701 atttctgatt taggttcccc gacagataac ccgccgattg agtaaccagg gaaatccatc
     5761 ccgactagat ctttcgcgct ttgacgacga agatcttcaa accctgctcc ttggatgatt
     5821 ccaaataatc cttgacggtc tggattagca tgagctttca atcctcgttc tgcccatcga
     5881 gacgtacgtt ctactgattt tttcacgtag tcgtaacttt catcgaatgg tggacattcg
     5941 tcaaaactca tcatgatatc agagcccaat ttattttgga tgttgattgc tttttctggt
     6001 gataaaaaca tacgcgaacc gttcaagtga tttttgaaat gcacgccttc ttcttcgata
     6061 ttccgcatat cacttaatga aaaaacttga aagccgcctg aatctgttaa aatcggttga
     6121 tcccagttca taaatttatg cagtccgcca gcttcttcta ctaagtcttc accagggcgt
     6181 agccataaat gatatgtatt acttaaaata acgccagcac ccatttcttt caactcttct
     6241 ggggacattg tttttactgt agctaatgtt cctactggca tgaacattgg tgtggggaaa
     6301 gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag tatgtttttc tttcttgatt
     6361 aaacgatagc gaatggcagg ttgcgtcatt catttcccta ctttctatat aaaatatctt
     6421 gacaactccc accatcataa tcgtttcaag tgctttttgc aagttccctc ggcttccttc
     6481 tactttattt aatattcttt tttttatcct ttttttatga ataacaaaaa aaagaaaaac
     6541 attatgacag aaatattttt tgatacatgg tatattggtg ttattaaaga aaaggaggga
     6601 aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa aagagagtct aaaaggacgt
     6661 tggggacaag ctgttttact aaatctaatc cctacattga ttggtattgc tactattctt
     6721 cttattatga ttcctattat cttgtttgcc accaccatat atgatggatc agcgagcgtc
     6781 tcagaagcga caacttcagg aggcggtagt tcaggaggcg gtggcggaat tatttccagc
     6841 atcattagcg ctttgttcat gagcggtatt tcttggactt atcttgatat tttacgtgga
     6901 caaaaagatc agatcgaacc attcaaagat gcatttcgtg gattcaaagg tttgtttatc
     6961 ggcggagtga tcagcttagc tgtgctgatc tcgatcttta caacattatg gggtttgcta
     7021 ttagttattc ctggaatcat taaaagctat tcttattctc aaagctattt tatttactac
     7081 gatatcgtcc aagaaacagg acaaacacct aaagtactgg atacgatcac tgcaagtcga
     7141 aaattgatgg atggttataa aggacaactt ttctggttag atctttcatt tatcggctgg
     7201 catatcttag cattagcaac tgctggtatt ggttatctat ggctcagccc atacatttct
     7261 gcaacaaaag cagcatttta tgaacaattg ccaaaagata tttaaaagag agtgtgactt
     7321 gtgaccgagg gggtggcagg agaacaccgt ttatcgagag gcagtaaaag aaacattcag
     7381 cccattagca aaaaggaggt gtgacaacat tcatgtcaca cctccttttt gtatagttcc
     7441 agtaaaaaga aaatcaactt agaaacagtt aatcattcct cttcttctgc ttccggtaat
     7501 cgatggaact tgatcagttc attcaccgcg attggtacga tagataaaat ccctaccaag
     7561 aaccagtgat tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa tggcagcaaa
     7621 gcaacagccg ttgtaatagc taatgccagt aacgccattt ctaccaaagc tttattggtc
     7681 gctaaattga ctttgaagat cgattggctg ctgcgtacat tgaacacatg cagaatcgat
     7741 gaataagcta agatcaagaa tgcaacagtt tgaccgacat gctgacttgc gtctacccat
     7801 gggctgatag tatcgacata actacctaaa taataaccaa acaaggttac gatggtgaag
     7861 acccctgcat ttatggcaat tttttgcaat aaacctcgag caaagatccc ttcatttttc
     7921 ggaatgggtg gctgatccat gatgccgtgt tccgctggct ctcttcctaa agcaaatcct
     7981 ggaagtccat cagcaactac attgacaaac agtagttgca cagctgtaaa tggtgctccc
     8041 catcctaaca gcatcgcaat caaaacaata aagatctccg aaatattaca gcttagcaaa
     8101 aagttgatag ctttacgaat attctgataa accgaacgtc cttgactgac agcgtctacg
     8161 atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc cacatctgtt
     8221 cctgtgatcc ccatggcaca cccaacgtta cttgctttca acgctggtgc atcattcaca
     8281 ccatccccag tcatcgctac gactgcaccc gtacgttgcc aagatttgac gatccggatc
     8341 ttgtcttctg gcgttacccg agcatagaca gagagagctt tgacacgcgc atctaattct
     8401 tcgtctgata attgatgcag ttctgatcct gaaagtgctt ctgacggatc attcaagata
     8461 cctaattctt tcgctatggc actggcagtc accacatgat ctccagtgat catgactgtt
     8521 ttgatgccag cctttttcgc tcgcttgatt gctcctttac tttctggacg tggtggatcg
     8581 atcattccga tcagaccaat caaacgtaag tctttttcca gtgcttcaga agaaatatct
     8641 tgcggttctt cgtcataagt agcataacca acagcaatca ctctcagtgc ccgtttacca
     8701 aagcgatcat taacgatcgc cgcttgttct acatcgcctg aactaaagcg cggagcaaga
     8761 acatcgaatg ctcctttggt gattgagatg tatttttcgc ccatttggtg aactgttgtc
     8821 atcatttttc gttcagaatc aaaaggaagc tcgtttactc gtggatattt ttcttctaat
     8881 tctgcctttg tatgataatt ctcttcaaca gcccttacga tggctacttc tgttggatta
     8941 cctttgataa caaggtcgtc atcttcttgc tcgaccgtca catctgtaca tagtgcagcc
     9001 attttcaata tttccatcgc gctgttcgtc attgaatctt cgatattcgt tacttcatct
     9061 tctttgctcc atacccggcg gacgcgcatt tcattttgtg tcagcgttcc tgttttgtct
     9121 gagcagataa cattagcggt tcctagcgtt tcaactgctg gcaactgtcg gatgatcgca
     9181 tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca acgtaacaat aacagtcaag
     9241 gtttccggta cagctgcaac agccaaggaa atcgaagtca tgaacatctc aaggagtggt
     9301 tctccttgca gttcaccaat cacaaatact aaagccgcag ccgctaaagc gatcatactg
     9361 attcgtttgc ctagctgatt caatcggacc tgtaaaggtg ttttttgcat agtattttcg
     9421 ttcaaaagat ccgcgatctt acccatttct gtagccatgc caatggcagt gacgatcgct
     9481 ttcccacgac ctgcagcaac cgtacatcct ttaaatacca tattaagttg gtcacctaat
     9541 gactcatctt cttcagcaat aaatgctgca tctttttcaa ctgcttcact ttctccagtc
     9601 aacgccgatt cttctacacg taattgtgat gcttcgatca aacgggcatc ggcagaaata
     9661 gcatcaccgg actctaaaac aagaatatca cccactacca gttgctctga ctcaacagtg
     9721 gtcactttgc cgtctcgtat gacagttgcc gtttgtttat tcatatcttg caaggattcc
     9781 agtgcttttt ctgcgtttcc ttcttggaca atcgccaata ccgagttgat cacaacgata
     9841 gcgatgatca gcagtccttc aaataaatcc ccatgttctg tagcaaatgc ggtataaaag
     9901 gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat cggacaagct tcgaaagaat
     9961 tttttgatca tcgattcttt cggcgcctca tcgaatttat tcaatccctg cttttttcgc
    10021 cgttcatcca cttcttgtga agacaaccca gtatctggat ctgcttctgt taccttcttt
    10081 acttcttcta ttgtctgctt gtattcttcc atcctattcc tcctttaaaa tcttcgtaca
    10141 aaaaaaggta cttattttac tataacatcc tttttttatt tacttcaaat aaagggctta
    10201 tcgattattt gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa cgctaccaaa
    10261 ggatgctatt atatagatgt aaggaaggag gataccatca aatagacaga agaatttctc
    10321 gatagaacag tgtaagcaaa caaagttgtt agaagtacag gtttcaccca gacatgcgtg
    10381 tagagtacaa acctataaat agtgaatgcg tatggatgtt tggatcacga agtatccgaa
    10441 acaagaagta atgcgatacg taacaattga ataacgtaaa cgtctgaaaa ttgaatagga
    10501 acaagagatg tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt taacatgaag
    10561 aaattcaaca ccgtgctttg catgtatgat ggaaagttac aatcatttct gtttatttga
    10621 atccttccga ggtacaagaa agccgagctt atcgagagc
//
LOCUS       DAYLZO010000097        10466 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.099, whole genome shotgun sequence.
ACCESSION   DAYLZO010000097 DAYLZO010000000
VERSION     DAYLZO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.099"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            158..2536
                     /locus_tag="ACGVJG_002277"
     CDS             158..2536
                     /locus_tag="ACGVJG_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFJ6558197.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            2597..3139
                     /locus_tag="ACGVJG_002278"
     CDS             2597..3139
                     /locus_tag="ACGVJG_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558198.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            3217..3549
                     /locus_tag="ACGVJG_002279"
     CDS             3217..3549
                     /locus_tag="ACGVJG_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558199.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            3550..4143
                     /locus_tag="ACGVJG_002280"
     CDS             3550..4143
                     /locus_tag="ACGVJG_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558200.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            4165..6189
                     /locus_tag="ACGVJG_002281"
     CDS             4165..6189
                     /locus_tag="ACGVJG_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFJ6558201.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            6189..7388
                     /locus_tag="ACGVJG_002282"
     CDS             6189..7388
                     /locus_tag="ACGVJG_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFJ6558202.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            7404..8048
                     /locus_tag="ACGVJG_002283"
     CDS             7404..8048
                     /locus_tag="ACGVJG_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558203.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            8059..8865
                     /locus_tag="ACGVJG_002284"
     CDS             8059..8865
                     /locus_tag="ACGVJG_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558204.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            8888..9117
                     /locus_tag="ACGVJG_002285"
                     /pseudo
     CDS             8888..9117
                     /locus_tag="ACGVJG_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9138..10211
                     /locus_tag="ACGVJG_002286"
     CDS             9138..10211
                     /locus_tag="ACGVJG_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HFJ6558205.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            10255..>10466
                     /locus_tag="ACGVJG_002287"
     CDS             10255..>10466
                     /locus_tag="ACGVJG_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558206.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKL"
BASE COUNT         3767 a         1599 c         2194 g         2906 t
ORIGIN      
        1 ttgagaatga gacggctaaa aaattgttaa ttcaatttta cgaagaacaa gcaacggatc
       61 gtcttcaaga atatgttgaa aagttgatca aaaatgaaga aatttctgaa atgattagga
      121 aacggctgtt attggaagct gaaaaggcgg tgaaagaatg atcattggag gaattttctc
      181 tggcatagcc gactggtggt caggtttatt cggagaaggg gatgcagatc ccaaaaccgt
      241 aacgaaagtc ttacaagatt atggtaatta tctgcagtat caagatgtat tcagttggat
      301 ttgtaatact tttttatggg gattgatcaa agggttatat aatctcaata ctttattaga
      361 aaaaacaatt tatcaaagct ttgatttaaa agacttattg aacgccgctg gtgttaatga
      421 actatttcaa agtttgatta gtaaggtatt ggctcttttt tgtgtcgtca cattaattta
      481 tttaggcttg aaatttttta tgagtaaaca cccaccgaaa gtcaaaaata tcttggttaa
      541 tttatttatg gccatgatct tgatttttgg tggtagttct ttaatcgatg aagggttaaa
      601 tatcagtaaa aacttttatg gagacgtcac tgcagcaaat aaagcagata aaacgggttc
      661 acctgctttt caaattattc aaaataatgt gattgatgtc agtacattac tagcgactga
      721 cccgaataaa gtagaatcct taccaacaga taaaagaaat gctttaacag cgaaaaattt
      781 caaaacagcg aatatcaatg ggattattac acctgatcaa gctaagaaaa tgtctgaatc
      841 aagtgatatt agtacagtac aaaaagatcg gttaaaggca ttacagtatc gactagagtt
      901 agacgataat gggaaagaaa tccctgttca aattagtgat gaaggtttag caaaatatgt
      961 gtatacgtca ggctatcgtc gatacattag tagtccaggg attattttgg ctggggaatc
     1021 ttctttgtcg gtagcctatt tatttatttt gtttacaatc gttacttgca ttattgagct
     1081 agtatttaaa aaattctatt tagtaattgc cgcgtcaacg gactttgaga caggacaacg
     1141 gatgaaaagt gcgatagaag atatttctca atccttcttg ttattggcgt ttacgatttt
     1201 agaattacga atctatacat taatgctttc aggtatcggt gatttgcatg cggtaggaaa
     1261 gatcaatgga ttcttgtatg ttgttgcttt aattgtgcta actattgcgc tattcaaagg
     1321 ctctcaatcg gttacgaaga ttttcggtgt tgatacgagt ctgaaaaacg gcagcaattc
     1381 tcttcttagc atgtttgcct taggaaatat tgcgaaaaat gtgggtagcg gagctaaaaa
     1441 ccttgcaagt gctggtaaga gtggtttaag ccatttaaat gatattcgta agaatggatt
     1501 tggccatcga aaagatatta cccatgataa cggggaagaa caatcagacg ataaagctac
     1561 aactgtacct aatagtaaga agacagtttc cgaaatgttc gggaaagcga aaaatggtgt
     1621 ttctaaagcc gcagaaggtc ttggttacgt caatgaacgt gggttaaaag gaacagttga
     1681 agatcttaca gaaaaagcca aagacacctt agaagagaaa ttagacggca cgagagctaa
     1741 agaatttgct gaagctgtga agaatccatc cgaagcacta gaaaaacaaa aagaaaaagc
     1801 ttcagcagtc aaagaaggac taaaagatgc gtatggtgaa ggacaagtca atgcagcagt
     1861 gaaggcaaat acaacaagtg aaaaagaaaa tcaagaaggt gcagcgattc cttcagccaa
     1921 agatcaccaa gatgagttac attctaacga aaattcgatc ggagaaaaag attcagaaga
     1981 aggacaacat ggtcagttac caaaaaccga tccagaacaa tatcgttata aatctaacat
     2041 taattctgaa aacgaaaaac gcatttcaga tttaccaaaa cgaaatcaag aagtacaaac
     2101 ggatgaggat tccatcgatc tgccaaatga taatggcatc gaagttggaa ctgaaaaatc
     2161 aacgaaaatt ggtttgccta agacacaaaa tttaccaatg aaagatagtc gtttgaatca
     2221 attcaaagag aatgatcaaa gctttgttca tcaatcacct gcaggtactg ttcaaatgaa
     2281 atcaagtcgt ttgcctgaaa atggaaatct gacaactgtt tcttacaatt tagctgaaag
     2341 tggtgaaaca gagaatgcgt ccgttcaaaa tattgtgaca aaagaagctg cacctaagcc
     2401 aatcaataaa gttcataatg gatacagacc agcaaacgta gcatttactg caccgacaaa
     2461 aataaatgtg acaagagaaa ctaaaaacag gaacaatgaa ttgaaagaac aactattgaa
     2521 ggatatcaaa ccataaaagg cctatttaag ggcctttttc tttataaaac aagtttaaaa
     2581 tggaaaggag aaaattgtgg aggatatttt aatagctacg tttataactg cactgggagc
     2641 atttttaacg atatttgtgg tgactattaa aggatttaaa gatacggata gtttacaaaa
     2701 agatcatcag tctattttag atggtcaaaa aacacatacc gaaagcatta ttgtacacct
     2761 ttcagaagga ttcacttatt tgaaagatgc tatgcccaca ttcacagtaa aattaacgga
     2821 aatatttcat tctgttgagc aaatatcgtc agaattgaaa gagcaaagac aacaaaaaga
     2881 tcgtcaactt ggctcactta attttaggca agaaacgata caagaatcat tggagaatgt
     2941 aaatcgatta aacgatgaaa tgcaacgagt tcatttggaa aatgaagaat tacgcgcaca
     3001 aaattatgat ttaattcaaa agaaaagaaa tctggaacaa aaaattgttg aattaaaaga
     3061 agaaaatcgt caattgaatt atcaattacg acgaggaaaa aatcgttctc gtgatttagg
     3121 aaatgaatta gagttgtaat atttagaaaa acgctgtgag aaatcacagt gttttttatt
     3181 tctaaaaata caaaatataa ggggtatggt tatattatgg ataaatacat tcatatgaaa
     3241 aatcctccaa gatttagtgg ggaatggaag tttttcgcct ttttcttttt agatctatta
     3301 acgcttggtg gctgcttttt ttgggcgaaa atagtcaata atcttgtcca cttaccactc
     3361 ttttttgcac tcttgaatta tggattatgg cttgcgatag cgttagtgat tgtttggcga
     3421 ccctataata accctggtca acgccaaatt tttgcgatta tcgaatctat tttctttacg
     3481 gataagaacc actatcatgc gttagatcct aaccgaaatt atcatggtac gtataaggag
     3541 gatgaataaa tgcgagtcaa aggacaaaat tcagttgata agaaatccat tttgcagttt
     3601 ggaaaaccag aagagcgctc tatcttggac ttatttccat ataaatcctg tacagaagag
     3661 ggagtcttaa ttactagaga agatcatttt cagcgttatt atcgtgtcgt ttccactgac
     3721 gtggagggat taaatgaaca agagaaacta gaacgaatga atcagttgac caccgtcatg
     3781 cgtacgtatg tgccaacaat taagttagtt agtttgacca cagagacgga tctaacggaa
     3841 caaatcgtgc aaaagcggca gttattagat aaaaatcgta tggaacagtc actaggaaga
     3901 aatctacagc gattaagaaa atatgaaaag aaattagtag aggagatcca ggaattaaaa
     3961 cgggcagaac aagaacgtcc tgacttaagc ttctttttcg ttattgaagg aaagacaatc
     4021 aaggatttac aaactagaaa tcgtcagtta atgcgatcta gtggcatttt agggttaaaa
     4081 ccactctaca aaaaagaatt gattaagatt ctttatcgaa tgaacaacat gaatgacgaa
     4141 taaaaaagat ggaggaaatg attcatgcta gatgtgataa aaacagcttt ttcaacgcaa
     4201 agtaatcaaa aggtgattga tccattaaaa caacttggta atcgtgaaat agagatttta
     4261 aaatcaaaag gttatgattt agatttctta aaacaagtga tgccacaagg tgggttgaat
     4321 tttgatgaag aatatgtgac ctatggaaat ggttattcac gatgtgtgac agtttatcat
     4381 tacacaaaaa atcctactct tttttggttg gccaatctca tgaacaatcc tcatacagtg
     4441 gccacactag atgttagcac agatgaaaag caaaaagtga ttgatcaaat caaccgcgca
     4501 ttagatgaat taaatgatcg atcagataat gaacgaaaag cgactgaccg aaataaaagt
     4561 gcggaagaat acctggaact agcggaatat gcccgaaaat tgacttctaa tggggaaatt
     4621 tctaaacaag tcaaagtgag agtacatgtt tacggaaata cgatccaaga agtggatgaa
     4681 cgggcgcagg aactgatcga aaatctaaaa gggatggatt atcgtgcggt gacttatctg
     4741 cataaaacca aacgagattg gcaatcgatg tttaccatgt tcgatgaaca agctgaatgg
     4801 tttggaaata tcaaagggca atcacttcca tcgttaaaca ttggcggagg attcccgttc
     4861 caccaccaaa cgctaaaaga tccaggaggc acacaatatg gggaaaccat gacaggcggc
     4921 ccatttgtct ggaatgccac gtataaaaat agtgtacgtt tatctaccaa taccttgctt
     4981 ctaggaatga tgggagctgg taaatcgacc gttcttaaaa tgatcgcgga agcccattta
     5041 gctgcaggag actttatttg gggctttgaa aagggaaaag actttattcc tttcttaaag
     5101 gaatataacg gtattatggt gcgtttagac ggatctgacg ggatgatcaa tcctttagag
     5161 atctttgcca cacggacgta tgatgaagca tccagtttat atgacgatgg ttcagtcaaa
     5221 gatttgaaga tcaatgaagc agcgtcctat caaacgcact tagacaaagt agtctatcag
     5281 gtacagttag tgagtcctca gctaaaagga acgatgaaag cagaattcaa gacctacttg
     5341 aatcgttttt atgaagagta tggaacagta ccacgcggtt ttacttcctc aaattcgcgt
     5401 tcaaatactg atacgcaagt cacagggaaa gatccagaag cttatccgac gttcaaagaa
     5461 tttttagcct ttttaggaca actggaacta ccaggggctt cccaagagaa aaagaatcgg
     5521 aaagaagaaa tggagtctat cgtggaatcc ttatgtgaga cttatggcat gatttttgat
     5581 ggtcatagta cgattcgaca ccttgaccaa caacagttgg tcttttttga cattgactct
     5641 attacaggcc tagatcgagg ggtacaacaa tgtcagatgt acatggccat gacgttgatt
     5701 tggaatcagg cattgattca aggacgaaga caacgacgct taattaaaga aaaacaaatg
     5761 gaagaacaag acttcaaacg cttcttggcg atgattgatg agtgccataa tgcgattaat
     5821 ccagatttca ttgaaacggt aaattatgtc acaaacttcc aacgagaaat gcgtaaagta
     5881 gaagcaatga ctgttttagc tacacaatcc ccacaagaaa tggtgccaga aaatatgtct
     5941 tctgataact tctcgaaact gaagaaagtc tttgaatttt cttctacgaa gatctttatg
     6001 cgtatggatg aatctatttt acgtcatata gaaggattag ttggtaaatc aatgacgaac
     6061 tcagaattag aatccattcc tcaacaaaat caaggagacg ctgtgatcaa ttttggctca
     6121 aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc aattaaaatt gtttgatgga
     6181 ggaaaataat ggaaaaccaa aatgaaagtt taatcaaaca gtatgttaag cgtcgtgcaa
     6241 agcggcgctt atttttgtgg gtgtttggta cttctgcagg tttgattact atattgatca
     6301 ctgtgtttgt gacccttttt ctaatacttg cggcaggttc tattgataat tctgattcaa
     6361 gtagtggtgg cgaagctttt acaggagaat attcggaagg cttacccatt tataaagaaa
     6421 tcaaaggaag aggccctttt tctgatgaga ttgcgcaata tgctgtaggt gcagcagtga
     6481 agtataaatt attaccaagt gtgatccttt cgcaatacgg ttatgaatca gcgtttggta
     6541 cgtcggcgtc cgcaagaaat gatctcaatt attttgggat tacgtggttt gatgggtgtc
     6601 tgtttcctaa aggaactgcg cgtggaattg gtggcattga aggtggctgg tacatgaagt
     6661 ttcccaactc aaaagctgcc ttctcctatt acggctttat ggtagccaca caatcaaatt
     6721 ttaatgcttg tgtcggaaat aaaagccctg gcgcaagctt attgatctta ggacgaggag
     6781 ggtatgccgc cgcaggaatt actgaagaca gtgcctatta taaaaattgt atgtctatta
     6841 ttaatacaaa taaattgatg gagtacgatg agtttgcgat taaacactgg agagaaggcg
     6901 gcggaactgg tggtacgatt acaggaagct ggggcaatcc attcccagga agtagcttag
     6961 ataaaaattc gttcagtgga ggacaacttt ttgggaaaaa tccaggagga gaattccgac
     7021 ctaacgggtt ccatgatgga ctagactttg gttcagtcga tcatccagga agtgagattc
     7081 atgcggttca tggcggaaaa gtcgtgtacg ttggcaatcc tggtatttca ggtttgggtg
     7141 cgtgtgtgat cgtgattaat tacgatggct tgaatatggt gtatcaagaa tttgcaaata
     7201 gtacaggaaa ttctcgagtg aaagtaggcg atcaagtgaa agttggtcaa gtaattggga
     7261 tacgagatac agcacacttg catttaggat ttactcgaat ggattggcgt caagcacaag
     7321 gacatgcatt cattgatgat ggtacatgga tcgatccgtt accatttttg aatagcagca
     7381 aaaaatagat caggagaaaa aatatggaaa ataaaacctt aaaaatcgtc ttaacagctg
     7441 caattagcgt tagtttaatc ggcaatattg cgttgttttg gcagcttaaa gaagaacaag
     7501 agacaaatac ctataaaatt gaagaagtgg ctaaaaacgc gtctgataag ctcaaaaaaa
     7561 attcttctga agcacaggcg aagattgaca agttaaatca aaccatcaat gagaaaaacc
     7621 aagaaatcga tcagctgaag caacaagatg gcacaaaagc aaaagaggaa gtaactgaag
     7681 cacaaagaaa gacggcagaa gagttcggtc aattggccat tgataaatca ttaagcagaa
     7741 atgaattaac ggaaaaacta aaagatgtcg caacccaaga agtgatcgac aaattaagtc
     7801 ctgtagacga tgatcaccaa catgatgatt acggaagtta ctcttttaca ttaggtgacg
     7861 tgcaaactta cgctcaaaca agcagtgtaa aagaggaaca aaattttgtc gtatttgttg
     7921 attatacaat cggcaatcct caattcaaag atgtcaaacc acaaaaaata aaaggtggat
     7981 taactgtcac tgaaaaacaa gtagatggtc aatggaaagt cacagacttt agttatttca
     8041 ctcgttagga ggaagaaaat gagtaagaaa aaaggagcaa gacaacgttt taaaaaacga
     8101 aaaagttatc ttagatggct ggggatcttt atgattctcg gctttttgat tcttgctagt
     8161 ccgatttaca cacataaaaa ggtagacata ccagatgaag taagcacgaa cgcattttta
     8221 aacacgaagg aactgacgat ggtttcaaaa gaaaaaggag cgaaccagca attagttttt
     8281 gaatttgcga tcgacgatga agatcagagc ttattaaaag aactagctaa tcttaattat
     8341 cgtgtagaag taaagacagc taaaggggat tatcaagcaa taaaagccaa agtgatcaag
     8401 gtgtctgatg attattttgt agtgaagtta gcacatgtac cagaagaata tattgctatg
     8461 cgattaacta ttattcctga aaaaattgat ccaaaggtgg atatgcaaga accgcaagat
     8521 ctgatttttt atattcacga agataaggtc aaagatcacg tgaaagacga agattatgaa
     8581 caacatgcga tcaattacaa agtgaaagga tacaagaaag aacaaaaagc agcacaaaaa
     8641 caaattgaat ccttccaagc aaccattgat ttgaatgaag aattagtgaa gaaactaaaa
     8701 gatcaattgc cttatcaagt cgcagaagat caggaaaata ctgaaaataa gattaatagt
     8761 taccagcaag aaattgaaac atcgaaaacg caaatgaaag atcaagaaga gagcattcaa
     8821 aaacttcaag aaaaaattga tcgtatgaca aaagaaaata tttagaagaa taggtggagt
     8881 aaaagaaatg aagcgagcga acttggtcgg caaagatact ttcgctgagc agaaagtatt
     8941 gattaaagcc tatctcaaag ttgtaaaagc taaagaaata tgtcaagaag tgaattatct
     9001 aacacaacaa tatctgagtt gaagaatggt agaaagagtt tagatacctt aaaattcaga
     9061 tcatttgaag ctctatatat agctgctaaa aaatatttta cagctgataa taaataaaaa
     9121 attaaggtgg gttctatgtg aaaaaaacgt atcaaaagtt tttatggatt agctatatcg
     9181 tcctatttat cgttgggttt ctcgtcagtg tgtccgtgat cgtcggaacg ctttttcttt
     9241 tgttctctga cgaagatctg gtacttaagg ggttaatgag tacgttatcg tgtgtcatgt
     9301 tactttattt atgtatacag atcaacgaaa ttatgccccc tcattggcaa gaaaagaaga
     9361 aagaatttaa ggaagaaatc gaaataaaat ccaatgactg gattgaatac acatggcttc
     9421 ctctgaaaag aaatagcaaa cgaaaggaat tttgggcatt gcaattatgg ccaataggaa
     9481 aactttttat ttatatggct tcacttatgg ttactttaat cggttatttg gtggtggtct
     9541 atggggtggc ctattacttg ttaagtgagc caaaacagat cgcggcgtca aatattttca
     9601 cggcagtaat gacattaatg tgtgtcatgt gcttttctat ttttagttac ttactcgtcc
     9661 aatttatcag gcaaaattta acaatgagta aacttttcaa aagtttattg gacttactta
     9721 tcttaggcag tatgttaacg actctaacag atcggttcga acaatctgaa aaaacggtag
     9781 aggggataaa gaacttctta acgacgattg atcaatttat aaatggatat atgtatttgt
     9841 ttgaagtagg ctttattctc actttgtttg gttgtgtatt tataaaactc attaaaagat
     9901 caatcgaaaa agaaatggtt attgatctaa ttccgacgct gagcggctat gagtattacg
     9961 ttgattttgg aaagtatgag gagttgatcc atacggatat cgttcacacg agtatccatg
    10021 aagtaaccgt taagggcatc agatctactt taaaagatgg acaggagacg aaaacaatcg
    10081 accgaagaaa agaggtctac aatttttata aagttgaagc aagtctacga tatggcattg
    10141 ggaaattgag gttagatggg agtaatattc aacaaaacaa accatatgtt ttaggaaaag
    10201 tggtgattta gaacttccta tgaaagatga ataaataagg agaatctttt gaaaatgatt
    10261 cttctttaca gaattgtgct tatagttggc acggagttaa aaatcttaaa gaaggagcag
    10321 ttattcaaag tgaaagaaca tcataatcag ttactatgta ttaaaacggg tacgatgaac
    10381 attcaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gtcgattcga
    10441 gagcaacata agcaagaaaa attaat
//
LOCUS       DAYLZO010000098        10456 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.100, whole genome shotgun sequence.
ACCESSION   DAYLZO010000098 DAYLZO010000000
VERSION     DAYLZO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.100"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..803)
                     /gene="cdaA"
                     /locus_tag="ACGVJG_002288"
     CDS             complement(<1..803)
                     /gene="cdaA"
                     /locus_tag="ACGVJG_002288"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFJ6558207.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRREL"
     gene            complement(992..4672)
                     /gene="nifJ"
                     /locus_tag="ACGVJG_002289"
     CDS             complement(992..4672)
                     /gene="nifJ"
                     /locus_tag="ACGVJG_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFJ6558208.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            4870..5391
                     /locus_tag="ACGVJG_002290"
     CDS             4870..5391
                     /locus_tag="ACGVJG_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFJ6558209.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(6090..6655)
                     /locus_tag="ACGVJG_002291"
                     /pseudo
     CDS             complement(6090..6655)
                     /locus_tag="ACGVJG_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6834..7490)
                     /locus_tag="ACGVJG_002292"
     CDS             complement(6834..7490)
                     /locus_tag="ACGVJG_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558210.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(8058..8993)
                     /gene="whiA"
                     /locus_tag="ACGVJG_002293"
     CDS             complement(8058..8993)
                     /gene="whiA"
                     /locus_tag="ACGVJG_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFJ6558211.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(9027..10025)
                     /gene="yvcK"
                     /locus_tag="ACGVJG_002294"
     CDS             complement(9027..10025)
                     /gene="yvcK"
                     /locus_tag="ACGVJG_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFJ6558212.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(10022..>10456)
                     /locus_tag="ACGVJG_002295"
     CDS             complement(10022..>10456)
                     /locus_tag="ACGVJG_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFJ6558213.1"
                     /translation="REKINESFKSKGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPN
                     PHYIPELRPLTGKDQPVYDYVMSFDETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIG
                     CTGGQHRSVALTERLANALGKNYKVNVTHRDKDKRKETVNRS"
BASE COUNT         2996 a         2310 c         1760 g         3390 t
ORIGIN      
        1 agttctctac gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta
       61 tctaatgtaa tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg
      121 acttcgctta taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata
      181 ctttctgata gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact
      241 gcaccgtcat gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata
      301 tctgcatcta atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata
      361 gtaattagcg caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag
      421 atcatcttct catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca
      481 agatgttcca gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg
      541 tacgtaatga cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg
      601 attcggataa caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc
      661 agcagttgga tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc
      721 aaaaaattca gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa
      781 agttccccga tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag
      841 tatatcatat gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca
      901 taattttcag gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa
      961 cttcagagaa ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt
     1021 gttccatttt tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg
     1081 tcgttttttc ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt
     1141 tcttgatttt ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt
     1201 cttggatagt cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg
     1261 aaaagcgaac ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt
     1321 ccaaagtcat tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc
     1381 aatagccaga agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca
     1441 aaccatgtgt gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg
     1501 cttcttcaaa agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat
     1561 aaacatttcc atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac
     1621 tagccgaaaa tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat
     1681 tggaatagac ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat
     1741 gatcgatccc gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc
     1801 tgttttttac gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc
     1861 gttcgctttc gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa
     1921 acatgtgtac caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata
     1981 cttctttgat tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat
     2041 aaccaaattc agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt
     2101 cattggtcgt ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg
     2161 cattcgcgat catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt
     2221 cgccacagcc tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca
     2281 agacagtatt ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc
     2341 aattcacagc ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg
     2401 cgggacaagc ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac
     2461 ggtattttac gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt
     2521 ccatttcctc ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac
     2581 attcattaca catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc
     2641 gcttttccat agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa
     2701 gtgtaccaac agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta
     2761 tataaccagt gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg
     2821 ggatcgcgac ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta
     2881 cgatagccat tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca
     2941 cctcatcaaa aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga
     3001 tccggcgtcc caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa
     3061 tgttattttc agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt
     3121 cgctccaaag agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat
     3181 agctatgcag gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg
     3241 ctgaacgtat cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt
     3301 tcgaatcata ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga
     3361 tagctgattt atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga
     3421 atgtttgcgg ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat
     3481 catccacgat ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat
     3541 caaaaactgc tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac
     3601 caataacagc cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag
     3661 gttctccacc tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat
     3721 gaggcaactt ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa
     3781 aacctgtttt ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa
     3841 ctgatcccat agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt
     3901 cataagaagt tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta
     3961 atttatcata ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt
     4021 tcgtaccact aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt
     4081 tctcctgatt gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct
     4141 cgtgactcgt acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag
     4201 caagatgggc aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag
     4261 caaatcctgt ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat
     4321 ttgtcgtcac tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct
     4381 tgtacatatt cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg
     4441 caccagtttt caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta
     4501 ctacttttac tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta
     4561 attcagccat ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat
     4621 aagaaatata tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc
     4681 atccttgttc atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt
     4741 tcttttattt ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga
     4801 caaacagtct aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg
     4861 tgttttaaca tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc
     4921 ctttatggtt catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg
     4981 tattgccgac aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg
     5041 caattgcagc gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac
     5101 gtgatgcacc ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa
     5161 atattcgata ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca
     5221 gtagataaac taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac
     5281 gaagctgagc aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt
     5341 gatgaatcta tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag
     5401 gagcgtagga caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg
     5461 gaagcagaag caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta
     5521 ttccgaataa atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa
     5581 gctgagcaaa ttatctccac caaataattt attcatattt ttgacttgcg catactaact
     5641 aattcttaaa ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg
     5701 gtgattcaca ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa
     5761 aaatagttgc ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg
     5821 tttattttaa acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg
     5881 gcaaacacaa atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact
     5941 cctaactcct ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca
     6001 cagcctagta tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact
     6061 ggctgatgaa atggattaat agatttcgat tattcaaagc gattttctat tggtggattg
     6121 tgttcccaac taacaagacg gattccaaag tcattatata actcttcaag agttaaccct
     6181 ttcctgttcc caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg
     6241 ccaagacctg gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa
     6301 atccagctcc tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata
     6361 attgttgtgc cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc
     6421 tacctgcatc tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc
     6481 tttccccatc tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc
     6541 ttggtggctt aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca
     6601 cagctataac aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc
     6661 gcaaatcacc tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc
     6721 tactgatcgt aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca
     6781 tcagctttta cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca
     6841 ccaaaattgt taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga
     6901 tttaacaatt cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat
     6961 tctttgtcat gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca
     7021 ctaggaaaag tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct
     7081 ggaaaataaa atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg
     7141 ctggctaaac gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca
     7201 tcaaattcaa ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca
     7261 agtctaaaag gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa
     7321 ataaatacaa taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct
     7381 gctcgaagta aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa
     7441 ataaaaagta ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc
     7501 cctttataaa catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat
     7561 aacgtattcc aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa
     7621 atcactccat ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc
     7681 taatccaatg aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt
     7741 aagaagagca gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc
     7801 cagttataga taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt
     7861 atgtacaaaa ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa
     7921 aacaatagaa gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg
     7981 aagaaaagca agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta
     8041 tcagtttttt ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt
     8101 ttgcgaattc ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct
     8161 aattctttca agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt
     8221 tcaggaaggg catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta
     8281 gaagccgcat caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc
     8341 gaatttctca tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca
     8401 ccaatcaatg ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca
     8461 ttgcgccgtt caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct
     8521 tggttatgct gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta
     8581 ttgactgaac ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt
     8641 cccataatct cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc
     8701 atcaacacat cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat
     8761 ttcatttttt tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa
     8821 tagatacgtc tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc
     8881 acaataccta aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct
     8941 cgatgaactt ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc
     9001 tcctcctttc tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat
     9061 tcttctacta ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg
     9121 gtggaaatca ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact
     9181 agatactcat catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc
     9241 aatactgtat cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca
     9301 tctgtgaaat gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca
     9361 gcagttgtct tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg
     9421 gtgaataaac ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact
     9481 tttcgagccg ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg
     9541 gttttacggt cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca
     9601 gcatgcaagg tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt
     9661 tttgctaata attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg
     9721 atcagattac cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg
     9781 aaaatatctt catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca
     9841 ccaggaggag ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca
     9901 acagtcacta ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc
     9961 agaccagtgc caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt
    10021 ttcacgaccg gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt
    10081 tatagttttt ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac
    10141 cgcctgtaca gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca
    10201 atacagtttc cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca
    10261 tcacataatc atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat
    10321 gagggttggg caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt
    10381 taaaaccaaa tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact
    10441 cattgatttt ttcacg
//
LOCUS       DAYLZO010000099        10437 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.101, whole genome shotgun sequence.
ACCESSION   DAYLZO010000099 DAYLZO010000000
VERSION     DAYLZO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.101"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..394
                     /locus_tag="ACGVJG_002296"
     CDS             <1..394
                     /locus_tag="ACGVJG_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFJ6558214.1"
                     /translation="TFEGNYVAKRITDATSEPIKYPIIPTIVYASPKLAEVGVTKSHA
                     SSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLIGAAVISEQADELIDDLTLVI
                     NQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      457..561
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            635..1378
                     /locus_tag="ACGVJG_002297"
     CDS             635..1378
                     /locus_tag="ACGVJG_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558215.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            1393..2385
                     /locus_tag="ACGVJG_002298"
     CDS             1393..2385
                     /locus_tag="ACGVJG_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6558216.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            2397..3134
                     /locus_tag="ACGVJG_002299"
     CDS             2397..3134
                     /locus_tag="ACGVJG_002299"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558217.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            3124..3846
                     /locus_tag="ACGVJG_002300"
     CDS             3124..3846
                     /locus_tag="ACGVJG_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFJ6558218.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(3930..4553)
                     /locus_tag="ACGVJG_002301"
     CDS             complement(3930..4553)
                     /locus_tag="ACGVJG_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFJ6558219.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            4948..5640
                     /locus_tag="ACGVJG_002302"
     CDS             4948..5640
                     /locus_tag="ACGVJG_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFJ6558220.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            5967..6464
                     /gene="dfrG"
                     /locus_tag="ACGVJG_002303"
     CDS             5967..6464
                     /gene="dfrG"
                     /locus_tag="ACGVJG_002303"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFJ6558221.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(6536..8488)
                     /locus_tag="ACGVJG_002304"
     CDS             complement(6536..8488)
                     /locus_tag="ACGVJG_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFJ6558222.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(8495..8731)
                     /locus_tag="ACGVJG_002305"
     CDS             complement(8495..8731)
                     /locus_tag="ACGVJG_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558223.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            9133..9423
                     /locus_tag="ACGVJG_002306"
     CDS             9133..9423
                     /locus_tag="ACGVJG_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558224.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(9695..10044)
                     /locus_tag="ACGVJG_002307"
                     /pseudo
     CDS             complement(9695..10044)
                     /locus_tag="ACGVJG_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            10137..>10437
                     /locus_tag="ACGVJG_002308"
     CDS             10137..>10437
                     /locus_tag="ACGVJG_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFJ6558225.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT         3438 a         1731 c         1997 g         3271 t
ORIGIN      
        1 cacttttgaa ggaaattatg tagctaagcg gataacggat gcaacaagcg aacctatcaa
       61 atatccgatt atacctacga tcgtctacgc cagtcctaaa cttgctgaag taggagttac
      121 aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg gatcttacca gttggttcac
      181 ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg acctttgacc agcaaaatta
      241 tttgattggt gctgcagtga ttagcgagca agcagacgaa ttgatcgatg atttgacact
      301 cgtcatcaat caaaaattga ctaaaaaaga gttggatagc tatatcatgg ggtatccgac
      361 attggcaagt gatctctcct atttattgaa ataatcagca tgataactaa agcgcttgtt
      421 gctttttcct tcttgtgcta cactttaaaa aatgaatacc tgtttctggg gatgtcgtag
      481 acgacatgag agtaagttgt gaacttagac ccattgaacc tgtcagttaa tactggtgaa
      541 gggagaaaca tagcagacgc ttgaagcggg ttactatgtc gtgcccattt caagcgtttt
      601 ttgttattca tattagaaga aaggaggtaa caaaatgaag aaaaatcttt atccagcagt
      661 cagtatggca ttcattttaa ttatttggca gattgttgtc atagtttgga aaatacctgc
      721 ctttatttta cccggacctg ctgctgtatt ttctgcatta gcggcagatg cacaagtatt
      781 actttcccat tctgtcacga cattaaatga agcagtgctt ggtttggtaa tagctagcgt
      841 tctttctttt attactgcct tggcaatgga ttattggaag tttttagaaa tgtcttttta
      901 tccgttacta gtgatttcac aaatgttgcc aatcatggtg ttagggccat tacttaccct
      961 atggtttggt ttcggtatgc ttccaaaagt gatccttgtt gtcttgataa gctattttcc
     1021 gatcgtagtt tctttttcgg atacattgag gaaagtatca aaagagcaaa tcatcttttt
     1081 gaaaacgatg ggggcagata cagggaagat ttatcgaata tataaaatac cagcgggttt
     1141 atccggcttc ttttccggtt taaaagtggc tgctacttat tgtgtcagcg gagcagtcgt
     1201 tggagaatgg ctaagcgctc aagcaggctt agggtattac atgatacgcg taaaaaacag
     1261 ctaccagatc gataaagttt ttgctgcgat catttgtgtc attctattga gtttattgct
     1321 gaatggcgct tgttcattat tgaaaaaagg gtattatcgt atgctttata ttttttaaga
     1381 gaaggggatc aaatgaaaaa attatggctt ttattaccgc tactgttgct tttatccgct
     1441 tgtggcactg caaaagaaac aagtagcaaa caagaaataa aggatttgaa aaaagtgaca
     1501 cttgttttgg attatgtacc gaatactaat catactggca tctacctagc aaaagaaaaa
     1561 ggctattata aagaggctgg tttaaatgtc caaatcatcg aaccaggaga taattcgaca
     1621 agtattggtc tagtaggtgc ggacaaagca caatttggtg taagttatca agaagatgtc
     1681 acttatgctc acgcagatgg acagaatata ccagtcaaag caattgctac agtgatcaaa
     1741 cataatactt ctggattcgc caccctatca gacagtaaca tccattcacc aaaagatttt
     1801 gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag aagctgtctt aaaagctgtg
     1861 atggaaaaag acggcgggga cttttcaaaa ctgacaatgg taggaagcaa tggagaaggg
     1921 ccagaaagct taggaaaatc ctcagatatc caatggtact ttgaaggttg ggatatgatc
     1981 aaagcaaaag aagcgggtat tgaactaaac tatatcccat tgaaagaatt agatgaacgg
     2041 ttagattact atactccggt aattattacg aatgatcaac tgatcaaaag tgatccagaa
     2101 ttggtacagt cttttatgga tgccacgaaa aaaggctatc aggaagctat taaggatcca
     2161 aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa atgatcggac gtttttagaa
     2221 gaatcacaag cattcttgtc aaaaaattat acggatgatc ctgagaattg gggattgatg
     2281 gaagaaaaag tttggcgcaa ttatacagct tttatgcagg aaaacggatt gatcaagcaa
     2341 gatgtacctt cgcaagaact attcactaat cagtttataa aataggagtg atagatatgt
     2401 caatccaaat cagccacttg aatgtagtca ttgaaaacct gaaaatatta gaagacatcc
     2461 atttcactat ccctaaagat acctttacat cgattgtcgc gccaagcgga gccggtaagt
     2521 caacattgct gaaaacttta acaggagtcc agcctatgac ttctggaacg atcaaagtag
     2581 acgatcaaaa agtaactggt ctccatacag catttagtta tatgccgcaa gaagatatgt
     2641 tacttccttg gctgagcgtc tatcaaaacg ttacgttata tcaaaaaatc aatcatctaa
     2701 caatcaatga agaacaagta agggattatc tggatatctt tgggttaaac ggctatgaac
     2761 attttttgcc ggaacaatta tctggcggga tgaagcagcg aacggccttg ttacgaacaa
     2821 tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt tggagcgtta gatgctatga
     2881 ccagagggca gatgcaagac tggatgttga agctgcctaa aaaagcaaag cggactactt
     2941 tacttgtgac gcatgatatc gaagaagcaa tttatttatc agacaggatt cttgtgcttt
     3001 ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc tgaaaaacga cggtcacgtg
     3061 aatggctact gcagcaatca gagttgaagc aaacgattta tcagttattg gcaggtgaat
     3121 tagatgttaa gtgatgctca ttgtcattta gatgggagtc agagtttagc actccttcag
     3181 caggaacaca cgattttgac gatcatcaat tgtgattcgc cagaagaatg gaaggaaaac
     3241 agacagcttg cagcaagtaa gactcaagcc ttaagttacg gtatccatcc ttggaaagca
     3301 gatagctata cgtttgaaca agttgagcct tttttgaaaa aagcaaggat catcggtgaa
     3361 atagggttag ataatatttg gacgaatgta ccgatgacta cacaaaaaaa agtatttgag
     3421 cgacagcttg cttttgcagc cataaatgag aaacctgttg tactgcacac aaaaggatgc
     3481 gaaaaagaga tcttaaatta tatccaagct taccctaatc actatttgat ccattggtat
     3541 tcttctttgg actatcaaca agactatatt gatttaggct gttatttcag cattggtctt
     3601 gatttgaaaa agaatccagc agtctggcaa ctagctaaag cagtcccaat cgaccactta
     3661 cttattgaaa cggatggact acatgcagca gagtgggctt taggggggat tttttcagaa
     3721 aaagattatg tctcactgct tgaagaacag ttaaagacaa tcgctgagat caaagagcta
     3781 tcagttgaag aagttaaaaa aaatacttat caaaacttgg aacgtttcat tagattaggt
     3841 aaataaaaaa gagatgtgac acaactagtg tcgcacctct ttttggcttt ctttgtataa
     3901 aagtaaactg tttgattaat aaatgaatct tagtaccaac cgtttgcttc ccagaatgct
     3961 ttcgccgctt cccaagaacc ataacgtgaa gtaacatatt gttctgctac tctttcttgg
     4021 tttgcagcag agtagtcacc atttaagtaa gaagaatcta attggtaacg tccgatgtaa
     4081 cgtccgttcg ttgctgtgta agaaccgctt gattcttttt gtgcgatcca ttcttttgca
     4141 gagcttgtgc ttgctggagt agcagcagtt tctgttacag caggagcagg ttgtgtttca
     4201 gcaacaggcg ctgcttgttt ttccgtttga accgtttctt gtacaacagg ttgttcagct
     4261 tggactggtg cttcttgaac ggctgcttga ggttcagcag ctgcttgtgc agagccttct
     4321 gttgggattg ttaattgttg tcctgagtaa atcaggttaa tatcagaaat tgaatttgat
     4381 tctgcaatgg cattgatcaa ggagttatca ccaacgtatt tttgagaaat cgttgataga
     4441 gtatcacctg attgaacggt ataagcttcg tccgcatgag cagtcgttcc catgaagaat
     4501 gcagcgccgg cagccaaagt tgttccaaaa agtaaagttt taagtgatgt catgaaaaat
     4561 ctcctttatt cgttaaaaag ttttatttgt tgtgttcaac gaggaatact ttaacacgtg
     4621 aaaatatttc ataggtaaaa gaagcatgac aatttatgac aaatccgcgt tttttgtaat
     4681 aaatacagta atctatgaaa tgaaaagggt tacgtttaat aaaacttgct tttttgtttt
     4741 tgttcaccat aaatatttat ttaagaaagt attaacctgc atttttctct ataaaaaaga
     4801 ttttttaaat agataaaaaa ggaggattaa atgagtattt gatagtattt atattaaaat
     4861 tacatatgta atttaaaaac gcttgacatt gtgtttgaga tcacttactt ttagcatgta
     4921 aaagtattgg attaaggagc tatgcaaatg aaaaagaaat ttattactgg tgggattgtc
     4981 gttgcatttc tatgtctaat tggctacaca ggcacgcggt acgcaaatga aattattttg
     5041 tggggtggag agacagatat tgatacaatc aatgagaatc tgacacagct tgatcataca
     5101 ttgacagata aagagcaagc gattacagat cttaccgctc gactagcaca aaaagaacag
     5161 acattgcaag attcaaaggc agatgtccaa gaataccaaa caaaagtaaa tgaattagaa
     5221 aaccaaaaaa atcaatcggc agcagaaaaa caaaacttag aaagtcaatt agtcagcaag
     5281 gatgcacaac tacaaagcaa acaaaatgaa atcaatgtca aactagatga gatcaaccaa
     5341 aaaaatgatg aaatcaatca tctgaatgca gattggacag caagactaca agaagcacaa
     5401 gcaaaacttt cagaattaca aaatactgta aatggtttga atcaacaaat cgcaacactg
     5461 acacaagaaa aaacaaatgt agaaactcag ttaacagata cacaaaagaa attagacgaa
     5521 gcaactgctg aaaactcaag cttgaaacaa tatattcaaa agttagaaaa agcaaaagat
     5581 gaagtggaag atacagctga taaatctcag caaattgttg aagaacattt gcgtaaataa
     5641 tctttcttaa caagtaaacc taaattagta aagaagcaac agtaatctat actaagtagt
     5701 tcagttttgg agtacaaaat gacaaaatgt acttcaaaat ggactacttt tttcatttta
     5761 acttacatac caaattacat acaaaatgat ttataaaatg caattgaaaa tgataattga
     5821 atgaaaattt gtgattttgt ggtattatga taatataact tatagaaaaa attgcttatt
     5881 caactaacgg gcaggataat gagaaaaggt tttttgatag aaataagaaa ttagatggta
     5941 aattagttaa aaaggctgtg ttataaatga aagtttcttt gattgctgcg atggataaga
     6001 atagagtgat tggcaaagag aatgacattc cttggaggat tcccaaggac tgggaatatg
     6061 ttaaaaatac tacaaaggga catccgataa tattaggtag gaagaacctt gaatcaatcg
     6121 gaagagcctt acctgacaga agaaatatta ttctgacgag agataagggg tttaccttta
     6181 atggttgtga aattgttcat tcaatagaag atgtttttga gttatgtaaa aacgaagaag
     6241 aaatttttat tttcggagga gaacagattt ataatttgtt tttcccttat gttgagaaaa
     6301 tgtacatcac aaaaatacat catgaattcg aaggagatac tttttttcca gaagtgaatt
     6361 atgaggaatg gaatgaggta tttgcccaaa aagggataaa gaatgataaa aatccgtata
     6421 actactattt tcatgtatat gaaagaaaaa acttattgag ttaacgggtg cgttaattga
     6481 agaagaacag taaagataaa gcacaagagc ttgggattac tccaagctct ttttgtcatt
     6541 tgaaataaat tatatctttc caatcaaatt gtttatctgt aatccctaat ctttgagcag
     6601 gtgtcaaaac ctttttatcc gctgatttaa aagggcgaca aaagttgtag taagttctta
     6661 gaattgtaat cgcaaattga gcatacttcg gattgaagtt agcgtaaata tagctcttct
     6721 tatcaccacg agctgttgtt aatggtcttt caagagatga tatataacgt cttatctgtt
     6781 gaataaagct attggtcgaa tggtcattaa catttaatag catctttgcg atttcgtttg
     6841 gttccagagc agataaatcc gttctacaat caactgaata aaatccttta tccttagtgg
     6901 ctaatggatg tttgataggg ttatcggcat atttgcgata tctttctcca tctttactaa
     6961 tagcctcttc gtggaattga tgacttgtta gtaattcagt tagataatga gatgccaaag
     7021 tccgtagaaa ctttgttttg aatcctctga tatctcccca atcgagcagt tctgcttttg
     7081 cttgtccgaa ttcttttaag cattgttttc tgcttttcgt cttgttaact tggcaaataa
     7141 aatgatgtgc atcgctcaac cttaattctt tcgtgaatac tctataaaag gcgtttctaa
     7201 tagagaagtc atcatcacta atcattcgcc attcgtcggc attaaccatt tgtttaatta
     7261 accaatagtg agcagttgta gtataaggag catcaacatt taaaccatcg acaaatctac
     7321 tcctattagt aattttccct aattcgtgaa gataagcatt cctattttcg ctatcgttag
     7381 cggatggttc ttgtggataa taagaccaat ctaacctatc atttttacga ctaaaagtat
     7441 tgagatggtc ttctttgaat tttcttgtat cttcatttaa ttcatccatt gaaatattcc
     7501 aatcataggc aacatcagaa cggaacacat atcttgataa gacctcagca gatacaacta
     7561 tatacgtttg catattaagg tcttcaaacc ccgtatattt ctttgaacct tgtcctttct
     7621 tacggacgtt atttaggtag tagtgcatct tgtccgtatt aagccacatc tcgttaaatt
     7681 tctttgtttg aaggggttgt gtctcatatc gttctaaaaa ctccaaacaa cgcctataca
     7741 gccactccag cttatgatag taggttccaa ctccaattcc taaaatatca caagtacgac
     7801 tgactgaaac ccttccaaca accatcttag cgaacattgg aagaatggtg ttcttttgtt
     7861 ggtgataagt agtggtttct tctctttttg gtagaacatt agtgaacttc ttacacgcct
     7921 tacattgata gcgttgagat ttgcccctac ttttccccct tttgtaaaat tgcttcggct
     7981 cattgaatgg ggttgattct tcttccgagc agccttcttt atgaaattga taatctggct
     8041 caacatcttg gatgctattg atttctataa ggcgtttaat ttcttcaaca acagaccaat
     8101 tagataaagg ggttactgta cagttttgcg atacacctct gccaatagga ttgtcattgc
     8161 agtacatacc tttatgacct ttatctttgg aactaccagt cattttatat cgacttggtt
     8221 tcccttttac atctttgaat ttgtgctgtt ctttcccaaa attacaacaa aacggattga
     8281 tgcagtaatt atattgaatg ggatattcag aaccgttcca tttaaaagac gttggcaaga
     8341 acaacatttt gttatacctt gttgcaaatc gttttggagt taataaatca tattgtttag
     8401 ccctatcttt taattcttcg tttgaaatcg gaattttaac atctacaatt tcatcttcct
     8461 tcgtggctaa tcttttaagc ttggtcattg tctatcaacc cactctatct ttgataccca
     8521 ttttttcaac aatccgctta ttacttcgct tgttggcaat ccattcaata aacttcttat
     8581 cgtctaaaat gttcaataag aattcatcca ctgcttcgct ttcagtacgc tctgcatatt
     8641 cagcgtattg cttaactatc acccttactt gctctgatat ttcccaatcc acagacttag
     8701 cgttcttatt ttttggctca agaaattgca taggaaaatg cacccctttt tgcttagtaa
     8761 attgtaacca ttttaacaca ccaatttgca ttcctacaat aattatttca cgttcgcatt
     8821 tttatataca ttttaactta cattttgtgg tgtaaattaa caacaaacgt tgatttaaca
     8881 ataaaaaacg agtatcaaat tcagttgcaa tttgatactc atttaggttt acattttcac
     8941 tttttgtact ccaaaactga actacttaaa tctatactgc tgcctctttt ttttagtaca
     9001 tgtccttcat cttcgcagga agtgccaata tgattttaag aaaggtaata gacaaaaagt
     9061 caaaaagata aaacagagtt tcttctttac aaagggaatg tatgttcgta taatggtatt
     9121 gaggtgaagg ttatggagaa aaaatatgga acagtagata agatccgtac tttgcaagtt
     9181 agtgccagac cgttgatccg tttcagttta aatggggtca attgtttgat tgcttcccat
     9241 agtttgaact tcctagctga agtagacgaa gggatgaagt tagtcgttac aggttattat
     9301 aataatagaa agcaatttgt tgtgagatcc tatcatgtgt tagggaaacc caaaattgtg
     9361 gtagaatatg agaaaagtct atacccaaga aaaaaggtcc gacaagatga aagaataaac
     9421 tagaaaacaa gcggctgaga caaaaatgtc ctagccgctt gttttggaaa gatggatgca
     9481 gacgatggga tttgaaccca cacgagcata cgctcacaag aacctcaatc tatatataac
     9541 tacatgtatt taagtatttg tgatgaatca ctaaactttc caaactcatt tgagggatat
     9601 tgcttaaaga ttgaacagat caattcgaag ccctattagt attcgctcaa tggggggatc
     9661 agaggccata aaaagagact agtcattact agtctcaggt agtggaattg taggatttta
     9721 aacgttcctg gagagtaccg gtatgtaact cgaagaggtg attatcatag tcgtaaaagt
     9781 aaattgaaga agcctcgcct ttcacacgag aacgtcctgg ttcgacagtt aaacctaaca
     9841 tttggatttt tgagacaaac gaatctatat cttgttcgtc aattttaaaa gcaatatgat
     9901 tgtatgtctt tggcagttta ttcgacgaat tctgcattac tgcaatccat aggtccttaa
     9961 ctaagaaaaa tttttctgga tataatgaaa atttttttgt tgactatcgt agatttcttt
    10021 tgcatcaaat aaggtattta acaattaagt atttaaaaga taacttttaa gttatcttta
    10081 agtaatgctt aaagctatgt tatactctat taggaaatat aagaaggagt ataaaaatga
    10141 acaaacataa tagaaaaata acgaataaca aagaactgat cttgttacta atagggattt
    10201 ggggtatctt tttactcttt aatatgtata tgccgactat gagagcggat gacgtagttt
    10261 atgcaagtag attagatgaa cttggctatt tgggtgcttc gatagaacat tataagacat
    10321 ggagttcaag aattattata gaactattct taatgttctt ttctaaacac tttatgctat
    10381 ggaaattatt aaattctacg attatgttag gaattgtagt attactttgc aagtatg
//
LOCUS       DAYLZO010000100        10389 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.102, whole genome shotgun sequence.
ACCESSION   DAYLZO010000100 DAYLZO010000000
VERSION     DAYLZO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.102"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..174
                     /locus_tag="ACGVJG_002309"
     CDS             <1..174
                     /locus_tag="ACGVJG_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFJ6558226.1"
                     /translation="VSFTHPTWKGSFVDVPGLGYMYSVDEDDEGVDKAMIGNWFTKVA
                     IPIEEAELSGGTE"
     gene            174..515
                     /locus_tag="ACGVJG_002310"
     CDS             174..515
                     /locus_tag="ACGVJG_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558227.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            757..3546
                     /locus_tag="ACGVJG_002311"
     CDS             757..3546
                     /locus_tag="ACGVJG_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFJ6558228.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            3536..4276
                     /locus_tag="ACGVJG_002312"
     CDS             3536..4276
                     /locus_tag="ACGVJG_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFJ6558229.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            4273..7035
                     /locus_tag="ACGVJG_002313"
     CDS             4273..7035
                     /locus_tag="ACGVJG_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFJ6558230.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            7048..7956
                     /locus_tag="ACGVJG_002314"
     CDS             7048..7956
                     /locus_tag="ACGVJG_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFJ6558231.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            7956..8576
                     /locus_tag="ACGVJG_002315"
     CDS             7956..8576
                     /locus_tag="ACGVJG_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558232.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            8580..9029
                     /locus_tag="ACGVJG_002316"
     CDS             8580..9029
                     /locus_tag="ACGVJG_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558233.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            9029..9523
                     /locus_tag="ACGVJG_002317"
     CDS             9029..9523
                     /locus_tag="ACGVJG_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558234.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            9537..9968
                     /locus_tag="ACGVJG_002318"
     CDS             9537..9968
                     /locus_tag="ACGVJG_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558235.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            9970..10107
                     /locus_tag="ACGVJG_002319"
     CDS             9970..10107
                     /locus_tag="ACGVJG_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFJ6558236.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            10145..>10389
                     /locus_tag="ACGVJG_002320"
     CDS             10145..>10389
                     /locus_tag="ACGVJG_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558237.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLV"
BASE COUNT         3713 a         1399 c         2190 g         3087 t
ORIGIN      
        1 gtaagtttta ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat
       61 atgtattcag ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt
      121 acaaaagtag caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa
      181 aatatcaaac aacgattaaa ttaacgaaaa aagacgaaga aggtaaatat gagcaagtac
      241 aatttaaatc tgctgaattt ttaccaggaa cagttgtaga agatgctgca ggagttatgg
      301 aagaaatgca aacagcgact gataagcaat cagttaaaaa agctttaagt cgtgcttatt
      361 catttattgc agatacttta tttgagggac aatttactgg tgaagattat tgcaaaggaa
      421 ttgatgctcg tgagattgcc tcattgacag gaaaattatt gaagtctgtt actgcaggtt
      481 ttgatgaaac ttatacagag acgaaaaaaa agtaagtgag gcgctcaagt caccttcatt
      541 taagtattcg attacttacc gagaattaga tataaagacg caattactcg aagcaggttg
      601 gacgttacca gaaattgaac gtactgactt ggatgagtta atgcgtcttt ttgcttttag
      661 agatgcggta aaagagcatg aagatgttga gtactatgat aatttcactc aattttagga
      721 ggtgatactt tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt
      781 actgccggta tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga
      841 aaaagtagaa atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt
      901 ctagctagcg gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata
      961 agtggagcgg ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg
     1021 ggatttagta attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt
     1081 attcaaggac ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct
     1141 tctacaggtg atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt
     1201 ggtttcggtg gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc
     1261 tcaaacggaa aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct
     1321 actctttctg ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggttta
     1381 tctcaaggta agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa
     1441 ggtggcggag gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa
     1501 acctctatac aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga
     1561 ttaaataaag cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg
     1621 ggcagttcaa tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca
     1681 tttttaagta atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg
     1741 ccctttcttg ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata
     1801 ccagcaataa taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca
     1861 ttaaagatta tgggggcaat agctgctgct aatccatttg ttttaattat tggtgcagtc
     1921 gttggtgcaa tagctgtttt cggttacttt atggcaacca atgaagagct tagaaacaag
     1981 gttatatcta tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag
     2041 gactggggaa ttgatacttg gaattctgct aaagaaatgg catcaaatgc agttgagggt
     2101 gtcaaagatg cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag
     2161 gatggggcca caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt
     2221 gtaaaaaatg catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa
     2281 gattcagcca gagaaaaatg gaatggaatc aaaggttcta ttatgtcagt tgctggtcca
     2341 ttgattacag gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg
     2401 aataatttag ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg
     2461 ccggttttgt ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg
     2521 attgctgttt ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat
     2581 atatttgtaa gttattttac aaatatttat tttgctgcac tcaatatttg gacagggttc
     2641 aagcttacat tgataaatat ttggaatgaa gtggtaaatc aagctaaatc gatttggtta
     2701 aacataaagt atttttttat taatctttgg attgatataa aatattttgc aattcagaaa
     2761 tggattgaat tgaagttttc tattattcaa acttggatcg atttaaaata taatgccatt
     2821 actacttgga ataatattaa acagtttttc aaagatactt ggaaaaatat taaagatacg
     2881 gcatacaata cgtggatttc tataaaaaat accatgatta atacatggaa taacattaag
     2941 gactctttct ggaatattgt tactggaatt gttaattctg ctgaaaatgc atggacaaat
     3001 ctaaaaaatg gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa ttcgttaagg
     3061 gaagtcaatt tatttgaaat tggcaagaac atcattgatg gacttataaa tggtgtaaaa
     3121 gaaaagtgga atgctttgaa agatactatc aaaggtattg caggtaatat caaaaattgg
     3181 attaaaggag ctttagatat tcattctcca tcaagatgga tgcgtgatat ggttggtaaa
     3241 aatattgttc aaggtattat cgttggtatt gataaagagc aaagtaagtt agatcaaacc
     3301 atgacaaatt tagtaaaaac accgtctgtt caacctgtag taaatggaat aagtcaccta
     3361 ccaattacac aaacaaagca aaatatatct gctagtgaaa caaaagaaat tcatttacat
     3421 ttaaatgttt atggagattt acctgattca atggttcgga agattaccac aaaaattaaa
     3481 acagaattga caagacaaat gaaacgagat gctggtgcag taggaggaac attatatgca
     3541 acttaagaga ggacaatttt ttattaatca acattattct tctgaattta atgtgtatat
     3601 tcaaaacagg cctgcttctg tttcagctag tcgagtaatt gaattgagag agcgagaagg
     3661 aaatgattct atcattattg ataaaggcta ttacaaaaat gtgactagaa agattgagtg
     3721 ttactataaa gcaccttcaa ttgatatggt gcaggagtgg gaagatcgaa tcactgaatg
     3781 gttagatatg tcgtcttata gtgattttat tctttactat gatgaacaat atatttatca
     3841 agctgtagta atagaaggac ctgaatttaa aggaacaaga aaaacaggaa atattgtccc
     3901 ttttgaattt acagtgagta ttcgaccatt taaggaaaat tacaatggta gatttactat
     3961 tcgacaaaca gaaacttttg aaatttataa cccagagaag tattcttcaa aaccgcttat
     4021 taaattgagt ggttctggag atgcttcttt ttatattaat aaggataaat atgatttgaa
     4081 atcgttggac agagaattat ttatagattc taaattagaa gaggcttatc gaaaattaga
     4141 tggtaattta gaacatcagg atcaggtcac cttattttta gattttccat ttttatatcc
     4201 tggaaaaaat gaaattaaat ggacgaaaaa tattcattca tttgaaataa tgcctaggtg
     4261 gtggagaaaa gtatgaaacc aagaatatat agtcctactg aaacagattt ctcaacgaat
     4321 ggcttaggaa ttttaaagga ttgtacaaga tgtggaatat atgaagtagc taatggaaaa
     4381 tatgaattag agttggatta tcctttagga actagatttg atgaatattt tgaaaatgac
     4441 tatcaaataa aagcaaagcc aaacgatcaa gaagagtatc atatcttttt tattgatgat
     4501 aaagatatag atactttttt aaatacagta actatttatg ctcagagtcg tacaaatcga
     4561 cttggaagac gggtagtcac tcttgtggaa gtggactcta aaactggtca agaagcgata
     4621 tcaattattg aaactaaaat ggataaaaaa tctgacatac gactttattc tgatattacg
     4681 gctgtttcta gcacaatctt tgaagcgaga aatgttttaa attgtattgc tggtgaacaa
     4741 ggatcattgc ttcaatattg gggcggagaa attaaacgtg aaccatttaa gctttctttg
     4801 ttaaagcgaa gaggtcgcaa taatgttgga acgattcggt atggaaaaga tatgtctggt
     4861 ttgaaggtca aattagattg gacaggtata aaaacaagaa ttattccata tgctgatcct
     4921 caaagtgaag taggtacgac taatcgaatt tatggttcgc cagtagatag tgcctatatt
     4981 aataactatc ctgatgtcta tacagaacat gttcagttta cagaagaaca aggagtaaaa
     5041 gacattaaga gcttaaataa aatagctaaa aattacttta aaacgatcaa tcctggctgt
     5101 gataagccta aagtttctat tacggttgaa tttgataagt tgactgatag tgaagaagcg
     5161 aaagaatttg cgaagattag aaattatggt ctgtttgata catttaaaat atatcataaa
     5221 aaatataata tttatcttga atcaaaggtt agtggactac aatacgattc tttatctgaa
     5281 aaaactttga aattagaagc aggagacatt caagttgctt tttatcagca acaagctgta
     5341 actattcagg ataaattaaa agattatgca acgaataatt atatgagtga ttttaatgat
     5401 tatgtttctt caatgattac aggtcaaggc agtgcaggag gatatgtagt tttatggcca
     5461 aaagaaaaac cttccaacat ctttattatg gataatccag atttagaaaa ggcaagagaa
     5521 gtactaagaa tgaataaaaa tgggattgct ttttctaaga aaggatggcg ggggcctttt
     5581 aattcagctt ggacattaga tagtatattt aatgccaatt ttatacaaac tggattaatc
     5641 aaagcagata tctttcaaaa ttcttttaac aagactggag atgtattgaa attagttaat
     5701 ggactactcc aaatttggaa caacaaaaag aaaataatgg agttaactaa aaaaggaatg
     5761 gaattttgga attctaaggg ttcaattgga acgattggaa caactgattc tgttggtaat
     5821 ccttttcctg gggcttctac tccaacccct attgaagata attctttagt tattcgtaca
     5881 aatggagacg gcaaatatat tttgatttct cctaaagtag gaaaaggatt agttttatta
     5941 ggaaatggta aagcaattta ttttggagac ttagatgtac aaggaaaact tacagttaaa
     6001 ggaaaagaaa taattcctgg acaaggtggt ggtccatcgg gaggtggaga atctactggt
     6061 gggtatccta gtgaattaaa aacagatgca gaaaagagag cttggagaat ttacgatatt
     6121 ttgtgtaata acggatttac gaaacaatct gcatgtggaa tattggggaa cattcaacaa
     6181 gaaacaggag gaacttttga tcctgatact gttcaaatag gtggaccagc atacggatta
     6241 gttcagtggg atggttcttc atatcctctt gttggtccag caacatggga tggaaaagtt
     6301 tatgttcaaa acttatttaa tgctgcaggt attaaagaac caataacgag cttagatgca
     6361 caagttcgat tgcttatttg gacatttaca aatggacaat ggatgggcgt agtacaacct
     6421 acgacggttg atggatttaa ggcctgtact gatccaagac aagcagcata tgcttttgaa
     6481 cgaaactatg aacgtccggc agcgacacat cctgaacgtc aagattatgc agttaactgg
     6541 tataacaaat ttaaagattt aaaaccagga ggagctactg gagaagcagg actaaaacat
     6601 ttagagtctt tgattggaca acgaattggc aatggtcagt gttatggctt gtctgcagaa
     6661 tattcaggat atctgggtgg ttgtggcatg ggtgctggaa caaaatatgg tttaactcat
     6721 gtgataggaa atacttctgc agcatctgat attggtattg cctatgattg gtctgctgtt
     6781 ggttggaaag tgattcaaaa ccctagatat gatcaattag tagttggtgc aattattaat
     6841 tgggcaagag gtggacaagt gggttcatgg tttgcagatg gaacttatgg acatactggc
     6901 gttatccgag gcttagctaa tggtcgtatg caaacttatg aacaaaacac agagttaggt
     6961 atgatttgtg gaaagttaga tcgccagtat tatagtgcaa gtgcaatttc ttccattgtc
     7021 ataccaccaa aatagggagg tgatttaatg gcaaagtgga atgtcatatt aagtacaaca
     7081 gaaccctata attacgtggg gatgattcaa gttcgacaag gcaataagaa tacagaggtt
     7141 atggaagcga ctatagttga aaatggtctt ccctacgatt tatcagaatg taaggtatat
     7201 tttgaatcag ttgtaggtgg gaaatatcca gtccaattag aaacaaaaat tgtggatgct
     7261 aaaaaaggga aaattaacta tatttttgat aaatattcca tgcagtgttt acatcgacaa
     7321 acagccaatt tcattatatt taaaggagaa gacttgattg gaacaactca agacttctct
     7381 tattttgtca ttaatgctgt ttcaaaaaca gaaggagaaa tgggttctta ttggcaatca
     7441 atcgaagatt taattgcgga tatgacggac tttattaatg aaaataaagg cgattttacg
     7501 gactggatga atgaaaggaa agaagaattt gaacgttgga gacaagaaca ggaacacagt
     7561 ttccaagatt ggagagaagg acaagaatca gactatttgg catggtttga atcaatcaag
     7621 gatattttaa agacgattga tccaggtgga gtcatgttag ccgaactaac ggatgcgcga
     7681 gtagatattc aaggagttcg tcatgcgtct atttcagagc gtttgctggc agatatggac
     7741 tatttatatc agaaattgcg agcaacgctt ttcacgattg aatacgggga gattgaagtg
     7801 actgatattt tgcaggatga tctcttttca gataatcatg aagtcgaaaa agttggaact
     7861 gtagcattcc cgattgaaga aggagccttg atcatcgcaa ccgttgatga tccaaagcaa
     7921 aatgtgttca ctcttgagaa agttggggtg atctaatggc taaaactaaa cgaatgatgg
     7981 aaacggatga aaaaacgggt gtacaacgcc aattctttcc aatcacacat gcttccgcag
     8041 ttcttgggtt agaagaaata atagcaggag aagcaacagt tttatctgtt aatggaaaaa
     8101 ttggagccgt cgtcattact aaagaagatt tgggattaga gaatgttctc acagaattac
     8161 cctatgcaag tgaagaagat gacggcatta tcacggcaga aatgtatcaa aaaattttaa
     8221 acagtggaga aggtgactac gtgttgccag ttgccactat cgaccgtttg ggtggcataa
     8281 aagttggtga attattgacg attgatgaga cagggaaagt ctctgcagtc agacaatctg
     8341 atgtcaattt ttcgctggag ttaaaagaaa aactcgattc actgaaaaat tatactgctg
     8401 gagaaaacat cactattgat gaagatggaa caattagttc tacaggtggt agtgggactg
     8461 gtggtgtcaa tcagtcatat gtagatcaaa aattccaaga agctgtaaat caagcagaaa
     8521 attacacaaa cgaaagaatt ccaaatttta cttttgaaaa aattggggag gtatagagaa
     8581 tgacagatat cgtaaaaata aaacaaagca atgtacaggt ttatccacaa actcattgga
     8641 atgctataga aggcaaacca acaacggtga agggagataa aggcgatcct ggccaagcag
     8701 caacaattac cataggaacc gtttctagtg gttcaaccgc ttctgttacg aatgtaggaa
     8761 catcatctgc agctagattt aattttgttt taccaaaggg agataaaggt gatcctggaa
     8821 taaatgccac aactacagct gtagctacaa ctacagcaaa tgggttgatg tcgtccacag
     8881 ataaaactaa gttggatgga attgcagctg gagcacagaa aaatccaggt aatgctacaa
     8941 ctacgacagc aggtttaatg tcagcaactg acaaagctaa actcgatgga ttagccaata
     9001 ttacatttga gaaagtgggg acggtttaat ggctgatatt gtgcaattaa aagaagacgg
     9061 agttgctaaa tacttaaaaa cgcatgtaga agctattgat ggtaaagaag cattggtgca
     9121 gacagatgga gaccaagcca ttgcaggaca taagaatttt tcaggttctg taacaatcaa
     9181 taataaacgt gttttgacga ctgatgataa cagatatgaa gtggtaaatc tggttgtcac
     9241 taacggtaat acagggacag caaagcttta tcgtgaagga aaaacagttt ctatatattt
     9301 tgtggcttta aacggaaaaa gtagtggcgg gaatgattcg gttattttaa ctgttccaga
     9361 aggctatcgg acaccaatta gttttgaaca actggttggg tcaatagacc gttctacttt
     9421 gaacagtgct caactatcta ttggcgcaga cggagccatt aaatggagaa gaaattcaag
     9481 ttatggatca gcttattcat ttgttatcac ttattcaatt taagggagga aatctaatga
     9541 aagtagttta caaatcaatc aagccttacg gattcgagca aatcattttg aacaatcaag
     9601 aaaatatccc tgaaaactgt acagagatta aaccaccagt tcctaactgg agaccaagat
     9661 ttgattttga taaaaaacag tgggttgagt tagctactga agaagagaaa aaagggaatg
     9721 ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg ctgaaggcag
     9781 aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta gcaatcaagc
     9841 aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat tcaaaaacga
     9901 tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa ttacaaggtg
     9961 ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg ctgtttcact
    10021 aacgaacaag taatggtttt cgttccgttg tgcattactg aagaagaagc agataaaatc
    10081 attagcaaag aagagagcgc atcttaattg atgtgctttt tattttgatt caaggagttg
    10141 tcacatgatt aatttagggg aatggggagc gatagcagga tcaataaccg ctatcgtttc
    10201 tttgatttta ttagtaataa aaccaattac tgcatctttc tcgaagatta ctgagactct
    10261 ttcaaaagta agtcacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga
    10321 tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata cacatacaga
    10381 aaaattggt
//
LOCUS       DAYLZO010000101        10102 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.103, whole genome shotgun sequence.
ACCESSION   DAYLZO010000101 DAYLZO010000000
VERSION     DAYLZO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.103"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(109..2229)
                     /locus_tag="ACGVJG_002321"
     CDS             complement(109..2229)
                     /locus_tag="ACGVJG_002321"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFJ6558238.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="ACGVJG_002322"
     CDS             2459..3637
                     /locus_tag="ACGVJG_002322"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFJ6558239.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="ACGVJG_002323"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="ACGVJG_002323"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFJ6558240.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="ACGVJG_002324"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="ACGVJG_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFJ6558241.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="ACGVJG_002325"
     CDS             complement(4990..6243)
                     /locus_tag="ACGVJG_002325"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFJ6558242.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="ACGVJG_002326"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="ACGVJG_002326"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFJ6558243.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="ACGVJG_002327"
     CDS             7821..9167
                     /locus_tag="ACGVJG_002327"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFJ6558244.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="ACGVJG_002328"
     CDS             complement(9475..9861)
                     /locus_tag="ACGVJG_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFJ6558245.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10102)
                     /locus_tag="ACGVJG_002329"
     CDS             complement(9910..>10102)
                     /locus_tag="ACGVJG_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFJ6558246.1"
                     /translation="FCLQSETVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSV
                     LLSRAQRRFEGTLRYSLGY"
BASE COUNT         3222 a         1842 c         2058 g         2980 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 tgtttcagac tgtagacaaa ag
//
LOCUS       DAYLZO010000102        10026 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.104, whole genome shotgun sequence.
ACCESSION   DAYLZO010000102 DAYLZO010000000
VERSION     DAYLZO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.104"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            141..398
                     /locus_tag="ACGVJG_002330"
     CDS             141..398
                     /locus_tag="ACGVJG_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558247.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1061)
                     /locus_tag="ACGVJG_002331"
     CDS             complement(630..1061)
                     /locus_tag="ACGVJG_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFJ6558248.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(1412..2110)
                     /locus_tag="ACGVJG_002332"
                     /pseudo
     CDS             complement(1412..2110)
                     /locus_tag="ACGVJG_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            2189..2365
                     /locus_tag="ACGVJG_002333"
                     /pseudo
     CDS             2189..2365
                     /locus_tag="ACGVJG_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(2541..3110)
                     /locus_tag="ACGVJG_002334"
     CDS             complement(2541..3110)
                     /locus_tag="ACGVJG_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFJ6558249.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(3184..3507)
                     /locus_tag="ACGVJG_002335"
     CDS             complement(3184..3507)
                     /locus_tag="ACGVJG_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558250.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3661..4473)
                     /locus_tag="ACGVJG_002336"
     CDS             complement(3661..4473)
                     /locus_tag="ACGVJG_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558251.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4800..5675
                     /locus_tag="ACGVJG_002337"
     CDS             4800..5675
                     /locus_tag="ACGVJG_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6558252.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            5689..6012
                     /locus_tag="ACGVJG_002338"
     CDS             5689..6012
                     /locus_tag="ACGVJG_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558253.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            6009..6761
                     /locus_tag="ACGVJG_002339"
     CDS             6009..6761
                     /locus_tag="ACGVJG_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFJ6558254.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6751..8922
                     /locus_tag="ACGVJG_002340"
                     /pseudo
     CDS             6751..8922
                     /locus_tag="ACGVJG_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(9034..>10026)
                     /gene="copB"
                     /locus_tag="ACGVJG_002341"
     CDS             complement(9034..>10026)
                     /gene="copB"
                     /locus_tag="ACGVJG_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFJ6558255.1"
                     /translation="NGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDA
                     FNKNEILQYLGALEANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQ
                     VKIVNEKEVERLGLNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFI
                     RTLKERKITPVMLTGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIM
                     VGDGINDAPSLARASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMI
                     QNLWWGAGYNILAIPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
BASE COUNT         3044 a         1914 c         1731 g         3337 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctcaaagtg aactgaaccc caaaagttga
     1321 actatttaat ggactgtttc cgatattcta ctggagatag tccattaaat agttcaactt
     1381 ttggggttca gttcaaagag ggggggattt tttatcgttg tggaatattt aaataccaac
     1441 gcttgtcatg aaaatcttgt gctccaccct tagtatttcc ttctggatcg tttgtcgcac
     1501 gcatcatgac gtatactttc ttattaggga aatcacgcat attgaaagat acatgatagc
     1561 caacatttcc taaagtatta taagcttgat ttacgtctgg tctataaatc ccatcagctc
     1621 ttactctagc tagttctttt ccagtattgt agtccataat gaaaatatac tcgtatttat
     1681 aattagcaat gtgccatcca gctacatgca agtttgcgtt ttcgatttct ccgaactgat
     1741 caatgtgggc gtaatttgtt ccatctgtca gcgtagaatt tgcagcacct gctcgagttg
     1801 gatcaattac tggtttatcc tccgaagtag ttggattatc atccgtaaaa ccatgagcca
     1861 aatcataggc tagtttttct ttacttacac ccatttcaga aagataaccg taaggatctg
     1921 tatgatcgcc ccaaatattt tgtgttaccc ataaatgcga tttgattcct ggttggttat
     1981 aaggagtgtc taatgttaat ggaataccat atttcattgc tgaatctctt gccaattcaa
     2041 cgtatacttt atagttcttt tcaaaagttt ctttatcatg tgtatgttgt aactcgattt
     2101 gcacaggact tttggctcac gaggttgtcc tactctacat cttgctgata gatatgctga
     2161 aaggaaaaca ccaacattat gttttgcttt ttcagctaga acgagacaag caggaatacc
     2221 tttagaagat atcaaaggtt ttttaggtca taaagatgtt tccactacac aagtctatgc
     2281 ccatatttct cccgaagtga aaaaaagatc aatgaatcaa ctagaaaact atatagaaga
     2341 gcaaataaaa aagcactcaa attgagtgct tttttaagct cactatcact aaaactatca
     2401 ctaattttat ttataagcga acatgtttct atcactgatt ttttaaatct agcgaaaaac
     2461 ccttatctta aaggcgttcg ttcaattctt tggcaaggtc ttcaaagcct ggcttgccta
     2521 gaagtgcgaa catggttttc ttatagctca tgtagaaaaa ttttcttgat gatgatttta
     2581 tccatcaatt gagattgact gttttgctct cctatgattt cagctatgat tgtccgattt
     2641 tgcggttttc ttcttaactg gtagttgatt ttcccagttt ccatcaactc gctaagcata
     2701 tccacgactc cgccaaatcc ggtctttttc gtaataaaac ctagtgctac cattcgttca
     2761 tctagatttt ttgctgtaat cgtcacacca gtaatgagcg agtgctgtaa tgtttgggcc
     2821 agctcttcca tcccttcagc attggatacg acggaagctg ttttgttaat aggttcttcg
     2881 tttatttctt ttggttcttt cctctccact ttcactgttt gacgaaggac atctagttct
     2941 tgcaagagcg tttcgtattg ttcttgggtc agcatcacgc cagctacttt ttcacgatta
     3001 aatatatata cgccagttcc cgcgtctcgc gcttgagcaa atacatccat cggcgatcgt
     3061 ttcacttctg tgatcgaaga tgtgggaact tctaatttct ttaattccaa tggatttcct
     3121 cctttactaa tcgcttttta ttatagcaca gactgacttg ttttgatctt ttttccaaag
     3181 gttttatcgg tttttgccta accagtaatt gatatcggta agaagtcttt cgataccaaa
     3241 tgaaggatgg tacagatacg ttttttgatc ttcgttatgg tctagtggaa aaaaatgatc
     3301 gatgatcact agatctgcat accgaggcgg cttatttcga gtacatacat gtatagagat
     3361 ccgttttcga tgtcttagcg ttttgttcag taccccagca agtcttcttg cttcttggtg
     3421 ttcatagcga aaaaaaaaga cgattttcac ttcaggaggg tggacatcta aataccctcg
     3481 ttcctggagt gttttcagta aaagcatccc ccactctact gcggggtgtg caagtgcttg
     3541 ttggatcgct agccgaaatt gatatagttt atctttggaa ttgggaaatt tttctaatgt
     3601 ttcctgataa taacgaactt cttctatcag ccggtcatgg ataccaatta cgtaacgaag
     3661 tcattcttgg taaagtactt ttgtcagctt tgatttgctt tcgctctcaa attcgctatt
     3721 cagccaaaac agcgaatcaa gcttttcagt cacaatcgtc aatttccgat agtcttgtgt
     3781 ctcttctatc cgtttccacc gcttttcagt aatctggtta cagctcaagc gcaaaattgc
     3841 tagataattt ttttctttta ttggcaaaat ctgctcctct ctaaatagca attcaggaaa
     3901 caattcaaag aatagtgttc gactgtctcc taaaatctcg atttctgccg gatcctttct
     3961 ttgcttcaat aattggatga acatctcaga aaaggactgc tctccatcat ctgcaaaaaa
     4021 atgcggaaaa tctttcaaaa agcaaaacag cagatatctt acttttcttt ctgaaccaaa
     4081 gatctgtgct tttttataag aaaaagaaag gtggtactcg ccaaaccact ttctcaatcg
     4141 ctcttgcgta gaacgatcgt acttctgctc gtaaggttca ttcaatagca gatttttcag
     4201 taaacaaaac gggggtgatt ggatcagaaa aacaaacaag agataaggtt cgctatatcc
     4261 ttgatcataa actaactgaa cggttttctt atgaagaatc agctgaatgg gacagtcgct
     4321 ttccgccaaa cgtttcagat gccgatccac tgttttaaca tcgattttca gttcttcagc
     4381 taaatcagct tttcgccaga tcaaatcttt cattagattg attagtgaaa ataaattctg
     4441 tgcatttttt gataatagtt tttgccatct catcgatcat ttctcacctg atttctaaac
     4501 atattgtttg atcaaaatgg gttcactcct aaaaagataa aaaactccct ttctatttat
     4561 ttaaatacgc aaatcttcct cattattttt tattttactc aatttaatcg tattctcatt
     4621 tcaaaacgaa tgtactttaa aaaaacataa taagatcact ttgcttgtag gatgtattcc
     4681 cttccattac aatgaaataa atagcgattt tatgagaagg tgatgtgaaa atggaagaaa
     4741 aaatagaaga atccaaacaa aaaatcgatc agttgatata agaattagaa aaagcggtca
     4801 tcggcaaacg ttcaactatc caattgatga tcgtggccct attagcaggt ggtcatgtct
     4861 tattcgaaga tattcccggt gtaggtaaaa cgttactggt caaagcacta gcaaaagctg
     4921 tacagggaac cttcagcaga gtccaatgta ctcctgatct gctgcctagt gatattcttg
     4981 gtttttccgt atacaattca caatcaaaag agtttgaatt tcgtccggga ccgatcttta
     5041 ccacgatctt gttagcagat gagatcaatc gtacgactcc tcgtactcag tcagcacttt
     5101 tagaagcaat ggccgagaat catgcaacga tcgacaacaa tacataccct ctagacaatc
     5161 atttttttgt cttagctacg caaaatccaa tcgagtacga aggaacatat cctttgccgg
     5221 aagcacagct cgatcgtttt ttattccgac tacaaatcgg ttatccttct tttgatgacg
     5281 aacttttgct acttttggat aaattcgaaa aagtgttgga taacttaaat gaagtattga
     5341 acagttatga aatagaagaa ataaaaacaa atgtggcgaa tgtctttgtc agtcccgaag
     5401 tggcatctta tgccttgcag ttagtgtcag caacaagaag tcatgctgcc atccagcttg
     5461 gcatcagtcc acgaggaagc ctttccttta ttcaggcagc aaaagcttat gctttaatcc
     5521 atgggagaaa ttacgtcacg cccaaagatt tgcaggattt agttccttac gtgttttccc
     5581 atcgcctgat tttttacgat cgttcattga aggaagacga aaagaaacaa atcattcata
     5641 ctcttgttgc acaaattcct attcctgtga ggtagattga ggtagacaat ggaaaaaatc
     5701 aaagattatt tcattaattt tgtgttattt ctggtatatt tcatcattgt gctttttgct
     5761 cttgtattca acaattcttt aggctggttc ttgttggcct tttttactct ttatgtatca
     5821 tggagcttga tttcattaag cagctttcta aagaaacttt cggttacttg tacattgcag
     5881 ccactcgttt atcgcaaaaa aagtatgcag gttatttttt ccatagcaaa aaagagaact
     5941 ttttctttac ctctccctcg tttacaaatc ggcttcccag caatctttgc agaaaaaaac
     6001 aagagatttt gattcttaca aataaaccgc aagaagtcca attgctttgg cagccaaaag
     6061 aaagaggatg ccatgaaaca ttaactgtca tttttacagc ctatgatagt ctaggacttt
     6121 tcagaaaaag tgcaaaaaga gagatctctt ttcctgtgac tgttctgcct gctttttgta
     6181 agattcaagc agaaaaatta cagagagttc tggaaaaaaa acagcagctg aacgcgtacc
     6241 aaagaggtct tgattttcgg gaacatcgcg cttatcgtcc aggtgattct ttcaaccgaa
     6301 tcgattggaa attgtctgct catactcaag agtggttata ccgtgaatac gattatcaag
     6361 aagaagaatg ttctcctctt ttttgctttt ggggttcgcc cagtccaaaa tttgaacctc
     6421 tgctagactt gtatttttct ttatggcatt tgagaaaaga aaagcagccg gaacttctga
     6481 tattaggcaa ccgcgccttt catggcaaag accccggtca tacctatttt gcttctttgg
     6541 aagctggcga tgagaaagct tttttcgcac acttgaaaaa atatgcaaaa aaacaaatcg
     6601 tcctctttgt tcctgagaat tctcctgaag tctcagaagc tattgaaaca ttgtcaaaag
     6661 atcgaacggt ttatcttgtt tatttcgtag aaaaacaact catggtccaa gaaaaagaca
     6721 agatatgttc attgccagga ggtgaatgga ttgacgatta aacgggaaaa atggctgcgt
     6781 ggcgtttact cctttgtgat actaagtgta ggtgctccga tatttgtagt agctaatcaa
     6841 ttgcaaaaca gctaccttct tatcctaact attggtgtcc tatgccttat tgtctgctgt
     6901 attcctaaaa aatgggtacg tttgctgctt tctttgccag taatgatagg ttgtttgttt
     6961 ttatattttc cttcgaagac attttccata ttatggatct tacagttttt ttcgtcagtc
     7021 acagaggaat tcttccatct atcccaaggt aaactttatt atttgccaga aaagacagct
     7081 ttttttctca taatgttgct gatctattta tttattacat tgaccgtgga ttacgactat
     7141 tggtacgcac cctttctttc tttgatggct tacttcatga tgcttaccgt ttttcggaaa
     7201 aaagatttac tgtacgaaat gattgcggta gtaactgttt tgttcgtgta tctggcagca
     7261 aggcaatttt tctccatccg gttgttgtcg ggaaacactc gattccagtc attgactaca
     7321 gttctgctta tttgtttctt aacttttagc gctatcttgc ctttatatct tccgaaagta
     7381 caccaatctc tcgaggaaac gagtgaaccg atccgagaat atcttaatga tgaagggctt
     7441 tatgacacac tccatgagta tcaattaacc ggaagtgcaa gaacaggctt cagtgagaat
     7501 gatgaacagc ttggtggacc tttatatgat aatggcgaag ttgtttttac agcgaaccaa
     7561 aaaggaaatg gctattggaa gatcgaaaat aaaaatcatt acaccggttc tggatggata
     7621 gaagatcagg acgcacccat ccaaccaatc caagaattcc ctgttgacat cacgagctac
     7681 caaaccgtta tagaagacaa agaaaacatt acattgaccc taaaaaaaca agttttctta
     7741 ccttatcctt atggaaatac taccttgact gatttttcat tagctagtgg acaaacgctt
     7801 tactatttgc ctgaaagcca gcgattcatt cctgtcccga atgaatcgac acgttctgca
     7861 actttacaaa cagaaaggtt agtttttacg agagaagaat taaaaagcag tcagttaaaa
     7921 gctgatgatt tagaaaatca gcagacaaat cttcaattgc caaaaagttt gcctaaacgt
     7981 attgaggact tggcggtcga attgaccaaa aatgaagcaa ctttaattcg taaagtcgaa
     8041 gctgtctaac agtatttaaa aacaagcgga aatttccgct attccaaaac ggataccggg
     8101 catactccaa aagaacacga ttatgtggat tatttcctct ttgattcaaa agtcggctat
     8161 tgtgacaact tttcaacttc tatggtcgtc cttcttcgaa cacttggtat cccagctcgc
     8221 tggacaaaag gttttacaaa tgggctcaga acaggcacag ataatcaagg gaatgaggaa
     8281 tacagcattc tcaatagtca tgctcattct tggccagagg tatactttcc tggatttggc
     8341 tggcttccat ttgaaccaac cccttcattt gccaatccaa tgggttcaca accagttgct
     8401 gaaacaaccg aaaccgtcca gcctgctatc gaacaaccaa atgaaagcga aaatatggca
     8461 gaaactattt ccagtgagag atctgaggtc attccatcag aagaaacaac actcacacca
     8521 gcttataaaa tcgtcttatt cagccttgga gtgtgtttat ccgtcttgtt gttctttcta
     8581 ttgttattca gaaaatctct gcgctggaca agttatctta tctatcttca tttttttgct
     8641 tctactaata cgattgcata tcaaaaactt cttaaccctt tggaaacatt ggtaccgcgc
     8701 aaacccgcag aatctttgca ggaatatagc ctgcgaatag aacaagaatt caagctagat
     8761 ctgcgtttca ttcgattaac tactcaatat gaagcagaaa tatatggacg acaagcagat
     8821 ccggtaaaat tcgataaaaa aacctattta gaaataacgc atataataag aaaaatggta
     8881 tacaaaaaaa ttctgacaaa agggagccgc ttcaaaaaat aagataaaat ttctggagaa
     8941 tctacttctg gaatgccgag aaccagctga ttgagtatcg attactcgaa aaaaaggttg
     9001 tgacacgttt tttgtcacaa ccttttttca ttattatctg attttcaatg tcaaagcatt
     9061 tagagctaca actaccgtac ttaacgacat caagacagca ccaactgctg gacttaatat
     9121 gataccaatt ggcgcaagaa ttccggctgc tagcggaatt gcaagaatat tgtaacctgc
     9181 cccccaccag aggttctgga tcattttctt tcgcgtttgc ttggctagat caagaaaatg
     9241 caaaatatct tttggatcgc tgtttgtcaa tacgacatcc gcagaatcga ttgcgatatc
     9301 tgtccctgca ccgattgcca tcccgattga agcacgggct aaacttggcg catcattgat
     9361 cccatcaccc accatgatta cttttttccc ttggtcaagg tatttctgga caacagcttc
     9421 tttatcatct ggtaataaac cgccataata ttcttcgatc cctaaataat ctgctactgc
     9481 ttgggcagcg tttttattgt caccagtaag catgacagga gtgatttttc tttctttcaa
     9541 tgttcggata aattcttttg cttctgtttt tactacgtca cctaaagcaa cgattgcagc
     9601 tagatgattt tctaaaatca ggaagctgat ggtatttcct tgttcttggt aaggtttcaa
     9661 taatgcttgt tctacgttta atccgagacg ctcaacttct ttttcattaa ctattttcac
     9721 ttgttgtttt ttgactgttg cctctagccc gactcctgaa agatttttta gattttcagc
     9781 ttgataaggc ttgatttcat gttctttcaa ataattcata atgccaacag ccaatggatg
     9841 attggcattc gcttccaagg ctcctagata ttgaaggatt tcatttttat tgaacgcatc
     9901 atcaagtact tcgacacccg ttacagtaaa ttttccttct gttagtgttc ctgttttgtc
     9961 cagcatgatc acatctagat cattggcttg ctccagtgca ttgcggtttt tcaaaagtaa
    10021 tccgtt
//
LOCUS       DAYLZO010000103         9666 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.105, whole genome shotgun sequence.
ACCESSION   DAYLZO010000103 DAYLZO010000000
VERSION     DAYLZO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.105"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..386)
                     /locus_tag="ACGVJG_002342"
     CDS             complement(<1..386)
                     /locus_tag="ACGVJG_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558256.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGV"
     gene            complement(642..896)
                     /locus_tag="ACGVJG_002343"
     CDS             complement(642..896)
                     /locus_tag="ACGVJG_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558257.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(921..2063)
                     /locus_tag="ACGVJG_002344"
     CDS             complement(921..2063)
                     /locus_tag="ACGVJG_002344"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFJ6558258.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(2123..3475)
                     /locus_tag="ACGVJG_002345"
     CDS             complement(2123..3475)
                     /locus_tag="ACGVJG_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFJ6558259.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(3546..3890)
                     /locus_tag="ACGVJG_002346"
     CDS             complement(3546..3890)
                     /locus_tag="ACGVJG_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFJ6558260.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(3900..4274)
                     /locus_tag="ACGVJG_002347"
     CDS             complement(3900..4274)
                     /locus_tag="ACGVJG_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFJ6558261.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(4287..4601)
                     /locus_tag="ACGVJG_002348"
     CDS             complement(4287..4601)
                     /locus_tag="ACGVJG_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFJ6558262.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(4715..7942)
                     /gene="fss3"
                     /locus_tag="ACGVJG_002349"
     CDS             complement(4715..7942)
                     /gene="fss3"
                     /locus_tag="ACGVJG_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFJ6558263.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(8021..8683)
                     /locus_tag="ACGVJG_002350"
     CDS             complement(8021..8683)
                     /locus_tag="ACGVJG_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558264.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            9300..9530
                     /locus_tag="ACGVJG_002351"
     CDS             9300..9530
                     /locus_tag="ACGVJG_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558265.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            9536..>9666
                     /locus_tag="ACGVJG_002352"
     CDS             9536..>9666
                     /locus_tag="ACGVJG_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFJ6558266.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL"
BASE COUNT         2664 a         2118 c         1645 g         3239 t
ORIGIN      
        1 acgcctttat cttcttcatg tgacaacatc accacaatat ctcccataac atattgttct
       61 tgataggagt aaaaggcgta atcttcgtgg agcatataat caaatttcaa atggagaatc
      121 tcttgaatga ttttcctcgc atccgttgtg ggaaagcgaa tccttacata gtcaaacagt
      181 aacgttaaag gtaactcggg attgaaactt tccagttggt caaaaatttt cctcatggtt
      241 tttgtcgatg cggttacgcc accattttct atctgattta agtacgggcg actaagtcca
      301 caagtcgtag ctaatttatt ttgtgaaacg ccatattcat tacgccggtc ttttaatagc
      361 cgataatcta aattcctttg tgccaagttc tcacctccaa atttaaggtg ttaacttttt
      421 caaatcagtt aacatctaca caaattgctt attatccttg ataggacagc cattccttat
      481 cgttgcttgc ttattttttg tgtttttgta cccccctgtt agatacgggg ggtgagacat
      541 catggccggc taacgccgtc cataaacagg cacagcacca cggaggattt ctgcttcgca
      601 aatcccccgc ggtcagtgcc tatcagtggt ttatttggcc gttactccgc ggtaataccc
      661 tcaatttcca actctgcttt tatctcaaac aagtattggc ggatatcagg ttctttagca
      721 aagtccgcaa ttcgttccaa ttcactgatg acttgattca gctccattcg agcatccgtt
      781 tcctcatcaa ttacatacgg atcatcaggg actttcaccc cgtttttgcg aatatcaaaa
      841 ttgagccggt acagttcttc ttccaattcc ggatctgact gaatcttgcc aatcacccag
      901 tagattcccc aatcaactca tcataaatca tctgttttta cctcctttaa tcttctagca
      961 atatcaaaaa tgaccggaac tgttacagaa tttccagcct gcttatacaa ctgactatct
     1021 gaattgactt gagaagctcg atcaaaagcc cagtccggaa agccctgaag ccgccagcac
     1081 tccctaggtg ttaactttcg aatttgatac tgatccgtga cgactgcttg ttggtctcct
     1141 gttaataagg tatgcacact ttgttttccc agacggcctc gacgacttgt ggaattagga
     1201 aaagccaggt tcacactgtt ccctggcttc acctcttgat attctgtttc gtttttccga
     1261 agcatgatct tcgttccgcc ttgtttcgtt gacaaagtag gcgctgttcc agttatctca
     1321 taaacccgat tgccttgttc aaaatttccc agtaacatac ccgctacaga aattccatca
     1381 tctaccggca atgcgatttt gattccttca tcaccggtca aggtaggcgc tacgtcatct
     1441 gacccataga cttgtccccc cattcctcga ttggaagggt tgatatttaa taagtttctg
     1501 atgttttgat cagatgttgg agacgatcca atgataggaa atacttgttc ggtacatgcg
     1561 tctcgagaat gtccgataag gaagatccgc tcacggtttt ggggaacgta ggcctttgag
     1621 ttaagcactt gccattccac atcatacccc aatccatcca gcgttcggag gatcgtctcg
     1681 aacgtagccc ctccttcgtg attgagtaat ccccggacgt tctcaaggaa taaaaactta
     1741 ggttggagaa tagcggcgaa ccgagcgatt tcaaagaata aagtacctcg agtatcggaa
     1801 aatccttgcc gctttcccgc aattgaaaaa gcttggcacg gaaatccacc gcaaaggata
     1861 tccactggtc cgagagattg aataaactcg tctgatacac ttgtgatgtc atgcatttcc
     1921 acctccttac ttgtatcatg gattgcctta taactctggc gagcaaaatc atcaatttca
     1981 caaaagccga tacactggtg acccgcttgt tccataccta agcaaaagcc accaatccca
     2041 gcgaataaat ctaaaaatgt cataaatcac ctcgtaaaaa aagcccagga ttcttgacac
     2101 tcctaggcta catactatta ttttatcctt ccgtattaac gtttaatcca ccaatgtcgc
     2161 taatttccct taagaaatca tatccttttg gtacaagtgg cgtataaaac tcactaatca
     2221 cactccctcc cgtatctaca tagcctcgac ctttgatacg cttcataaag aattttttat
     2281 caacctcgcc aaacatcatg gaatatccca gttcactcat acgacccaaa gccacccgga
     2341 aattaaattg atcgcgaatc ccgtctccta aatatttcgc atccggtctt tgacaagcta
     2401 gaatcagaaa gaaaccagat tgacgaccta acatcacgat ttgtttgagc ttattcaaaa
     2461 tcaccgcact ttccttcgtc gttaacattt ccatataaac cacgtattca tcaaagatta
     2521 aaaagtttgg tggaagtcca agaaacgcat aattctctcc tgttttgtag ttgggcattt
     2581 ctttcattgc cttgctacga gtcatcatgc gctcatagaa atcttccaca caagcagaaa
     2641 tttcctcctt ttgggaatag acatgaggca ttaccgtacc taaatcggct aagtccgcat
     2701 ttttgggatc aagaacaaaa agctcggcat cagattttaa taaagcctcg atgatagtga
     2761 gtaaaaagta agtcttccca ccacctgtgc cgccagcgat taacatatga ggtaaagaat
     2821 cataagccca tacttgattt ttcatcaatc gcaaagcccc actttctgcc actacttcgt
     2881 ctatcccaat tcgattggca attatatcat acagtaaagt gtactccaca taggagtctt
     2941 tgagttcctt ttctactagt tcacaatata gaccactttc taacttcttt tccagcttta
     3001 aaagctgatc ttgatatttt cccagtgaaa tttgaacgcg aatagaaagt aaaccatcct
     3061 tcatacggta atagattttg gggaagtaac tgattgtctc tttggttcga ctactattga
     3121 ggtctttgaa aaatccttca ctctgagttt gttttacttc gtaccaatga ttttctagaa
     3181 tcatccgggc tagcttttgt cgatgccaca aactgcgcca atgatcctga aaaaagtgta
     3241 tataaagcaa catcgtgctt cctgaaagaa cgagacttcc tagcaaatag attaaatctt
     3301 ttatagaacg aacgatttct atagtagaaa gtgatagctt agaaatatca aattgtctaa
     3361 gctcttgaaa ataaaagacg ctcatccaac cgatgaacag agcgagtagc cagccaagaa
     3421 caaagtgata aaccaagtgc tgatctcctg cccgtatacg atgccctttg tacattttta
     3481 tggtttccac ctccaatcga aaaaagcttc ataggatttc attgatccaa tttcaagcag
     3541 ttatgctagt catttactgc tatagggagt tgtttcgttt ccactagttg ctttaaaaaa
     3601 acttcaatgg catccggcat ttccacacca atttcttcca atacggtttc ggcttctcga
     3661 catagacttt catttacttc gataaccata aagaaggact cttcttcccc taaccaattc
     3721 agtacatcaa aatcttcttc gacatcgtta cctacttcca gtaacccttg atgcataatc
     3781 agaaacattt tttcatcata ggttaatgct ttagtgagtt gttcctccgt tagctcacct
     3841 tttccatatt gatcgagaca atctaaaata tgttcaacat taatttccat acgcttcacc
     3901 tatttctttc cttcattttg gtttgtagaa tttccctgat ctggttgtcg tttcaagact
     3961 aaatcatcgg ccttaatgaa ccaattgaca gtagttcctt ctcggaaaac ataattggcg
     4021 accgtatcca tgattggatt gaccaactct acctctgcgt cataggcaaa ctcgcgtaag
     4081 ggtacatttg ctgggatact aacttggatc attcgacctt gcgcagagga tttcaaatta
     4141 taagtccgtt cttttacctc gttactaacc gttccatcct cattttgctt ccgtacttct
     4201 cggcgtaatg cggaaaatcg taatttccca aacgttagtc ctgaatctac aacaatacct
     4261 tctgttaatc tcatcttctt ttcctcctat gctttcacta aatcatcgac atacaacacg
     4321 tagtcggtaa acccacgatt ctcaatctca tagcctttgg caaccaaatg gggattcacg
     4381 gcttttagtg gttgatcttg atcaaaatcc ttttcgccgg cttccgcagc taccaccact
     4441 tcaatatcat cggctcgttg cttactggaa tacaaatgat aactacgacc aattactgta
     4501 gtattccgcc catatccttc agttaaaact tcgccttcgc cggcatacct tatctttcca
     4561 aaggtttcgg ccatatctgg cacaacaaat ttcaattcca tcttcttttc ctcttttcat
     4621 ttttagtttt ggatacaaaa atagcgcgac cacttttttt aggaggcggt cgcgcacgtt
     4681 ggtttttcct atttagttgt ttcatcacat caccttagtt tcggcgattc cagaaataat
     4741 acccagcagt cgcaaagatc aaggtaaacc caataaacag cagaacatta gaatttttct
     4801 cccctgtttg tgggaatgtc tttactgtag gactagattc ttgagagtca cttggaacag
     4861 ttggtatttc cggttgttct ggtgcacttg gtgttgtcgg aactttttta ggtgttaaag
     4921 tttgagtttt ttcttttaga tcctcattgt gtttcccatt gatgtttccg tctttgccat
     4981 agttaatttc tttgaaggta aaacttgtac cttctggata tttacttgtg tccacttttt
     5041 ccgcaagtac ggttttagtg aattctttgt cattcacttc ataatcaatt ttgccagatt
     5101 tccaaatttc tttggtcgta ttatccggaa gtaatgcata cagaattgtt tcaaaagctt
     5161 cttttgagcc atccagtaca tcatgggtaa tcgacacatc atcgaacata tccaccacat
     5221 ccccatgagt aaaggtttgc gaaccatctt ctaggtgggc ttttgtttgg atggaaacat
     5281 ggcgttccac cgtacaattg acagtttggg cttgattgtt caagctagca tctttcgcta
     5341 ctggatcatc acctgcttca tagggtttct tgttttcata cacatagttg aataagacga
     5401 tggttttatc caaaacttgt tctgcggtta atttatgcaa aattttccaa gttcctgttt
     5461 tctctttatt cgccaacgtt gcggttgttt cggcaattac cactggtttg gctttttcat
     5521 ctttttcttg ggcagctttt gtcgcttcca catcaatggc ttgcgctacc acataccagt
     5581 cttctttgat ttcatgcaag ttatagctca atttatcaac tagctcggta gaatcaaggt
     5641 ctgtcaattc tttgcctccg tctttccaag tcccaagaga agttaagcta tcttcttctt
     5701 caggtaaatc atagagcatc aaacgattca agctaacaga aaatgcttta ttcgtcagtt
     5761 tctcgtaagg aacggtcacc gttttaattg gttgtttttg tcctttttcg gtaatcgtaa
     5821 aaatgtattc actcttagtg taatccgctt tattttcttt aaaagtagaa cggatttcta
     5881 atggcgtaat tttttgaaat ccttctggag cctctacttc ttcaagtaaa taatccccat
     5941 aaggaagatt ttcaaacttg ccatagccat caaaacctag ttgctcgtta taagctgtaa
     6001 ttgcttcatc ttcggcacct gtaatctcac tggttccttc caatggcgat actttaaagg
     6061 tcagatcatt gaagccagtt tcggcagtcc cagcagctga tccagcaaat ttaaagaaat
     6121 caaagccaaa gcgaatgact tgttcttttg ccgtaacatc tcgggtaatc acggcatttt
     6181 tttcgttatc atccaccttt ttgatggaaa cagggtactt tgtttcatcc aaggtatatc
     6241 cttcaggtgc tttggtttct tgccagtaat actcattaat cgctaagtgt ttaacagcaa
     6301 cttgattctt ttcatctaaa gtcaaagtca ccgtttcatc agaagctttc gttcccttca
     6361 ctaactctgg tttaaaagcc tcactccatt tgaccgcttg accatctttt gcagtaaaga
     6421 gagtatattc agctccttta aactcagctt taccttgagt ttcattaccg gtatctttgt
     6481 cttcttttgt caaagtggtt tcaccagtga tttcttggtt ttgccctttt acgttactgg
     6541 taacaagagc cacggtttga ttgacatagt tcaattcgac ttttactggt ttaaaggtat
     6601 tcacaaagcc attactagct ttggtttcgg tcacataata ggttcccaat tccaacgcat
     6661 tggcaatctc ttttggtgtt tccgcatgac ctttttcatc cgtggtcatt tcttggacaa
     6721 tttcaccggt tggactgtct ttgcgaatgg caaacgtatt tccggctaat gagtatttgt
     6781 cgttccaaag atcagttcct gtttccaccc ccattttatt caaaatgatt tgaccttttt
     6841 cgcgagtatt ttttgaagtc acagaaatag tctcaccaga tttaatggtt gcggtgattg
     6901 gtgtagtgtc aatcgtataa ggagctggca ctgatttttc agtaatggtt actttcgcgc
     6961 catgaggaat ctcatccaat gtcgcaatgc cttctttatc ggtggtcacg tcttttgcag
     7021 gtaatgtttt tccaaagtct aaatggaaaa cggttcccgg tacaacattt cctgtttctt
     7081 tatcgacttt tttaatcttc aaagaaccct cagtttccac atcaattttg attgtgtagg
     7141 tattgggctt atcaattgcc cccgccatca aagtttgttg accagccatt ttataagcta
     7201 ccggtgttcc tgtattagct gacttcttta gggttaattc accactctct tttgaattgg
     7261 catttggagt aatcactagt tgattgccgt tcacacgata atcaatattt gcgatattct
     7321 cgacaacttc atcaaactcc gataaattca agccgttcgt atctgtcaca gtagtcgatt
     7381 gacctaacac ggttttcact gtggagttat ggaagcttgg tttcttttga taatccgcaa
     7441 tggcttgatt gatttttgac tcaatggctg cgatatttac tgcactacca tcagaacgtt
     7501 taattgagtg aagcgtgtag ccattaactt cttgccaaat catcttctgg gccacaatat
     7561 gtgtatccac atccgtacca gcttccttcc ataaaacgga aactaatttc gctttctccg
     7621 acatatcagg caatggattt ttctcataac caggagtgaa ctcattgtaa atcggcaccc
     7681 ctggctcaat acagaaaata tcttgcttca caccattgta aactgcatag tgcggaacgg
     7741 taacttctga catgggacga ccatcgctaa atgtaccttc gacagcatac tggtgagcca
     7801 aatccaattc cacagttacc gtttgtggat gagtaatttc atcagcaaag gcaatcgttg
     7861 ggccaagcga tccaccaatt gtttggcata atagactcag tgttgcgagc aaacgccaat
     7921 tcttaaatct tggctttttc ataaaataaa tctcctttcg tttttgggta ataaaaaaga
     7981 cgtccaaatt ttaattttga acgtctgatt tagagtgata ctatttagtt ttctttgtct
     8041 tggataggtt gtttctctgc ttataaattt tgattcttcg ataatcaaat ccaatctctg
     8101 ccatcaattc ctcccaattt ttgttcgttc ttttcataat tgtatcaact gatggtagtt
     8161 ttggggattg aactagtttt cgaaactttg tcattgataa atcatcctgt agttttaatc
     8221 ggattatttc attttttagt tctaacagca gttcgaaatc agtaaatcga gatccatcat
     8281 ttttttcttt aaaaagtgga cgtatatctc ctaacatctt tttaatggta ctaagtgaag
     8341 gaatattctt ccctactgat ttttgttcgt acattcgttg agaggttatc ttctcgcact
     8401 taataaaggc ttctactatt tccaataatt cttgttctga cattctagcc cattttccat
     8461 acccagtatt ttctcgtcct atactaatca ataaattctc ccatgatcca tattttttgt
     8521 taatgaacca catacttggt gcagaatttg gattttctga tagctttttc tggtattgac
     8581 ggctagatgt aattccgtgt ttttcgatga attcattaat tgttaaaatc agttttaact
     8641 cactttgatt tttccaaatg atacgtccat cattttgctt cacgataaaa actcctaata
     8701 gttatttaga tagaagccta tcacatcaac tttttagtag aaaagtacat gttttgtctt
     8761 ttttgctaat agctgaccct ttgagaaact tgggttcaaa agttgctaaa ttcggtaaat
     8821 gttttggtat ttttcgtatt atatttgtcg tcaagctgac tactaaattt tgccctattt
     8881 tgacgacact gatagtcaat gtcggacaat ctaggacaat ttttaaggga atatgattga
     8941 aaaatggtcc aaaacagcca aaaatggctt aaaactgtga tagagaaaaa agaaaaagcc
     9001 cttgatatac aaggactttt cacaaatttc agatttccaa ggaagttttc cgtgtttatc
     9061 ttctgatttc tttgatacga gcagcttttc cgtgtaatgc acgtaaacaa aaccactata
     9121 ttctattatg tatctaacac cctcaaaaca gctataaatc aacaataccg acataaaata
     9181 aacactatgt tcaatctgag atatatcaaa ggtaaacgca tttttgcccc ctttttgccc
     9241 ctcaaatatc gctttacaac aagaacgtaa gttcgtataa tcgttttgag gtgattttta
     9301 tgatggaaga atttattaga aaaaatatca gtgatgaata tgcagacttt tatgatcaaa
     9361 gcagtaaaaa agataaattc cagatggatg tttcaatctt agctatatta gctttttccg
     9421 aaaataaaca acctgtaact gcaaaaaaag aaacagtatt ctctgaaggc aaaataaaaa
     9481 ctcgatatat attagaggta gaaactaatt ttaaaaacag atcggagtaa tggttatgct
     9541 ttttaatgag acacaattat ggtttaaatt tgacccttcg aatagattta tcaaagattt
     9601 ttataaagta tggaattcag aagttttctt tttagcaatc gaagatagct tattaattaa
     9661 tctcta
//
LOCUS       DAYLZO010000104         9598 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.106, whole genome shotgun sequence.
ACCESSION   DAYLZO010000104 DAYLZO010000000
VERSION     DAYLZO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.106"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(136..1302)
                     /locus_tag="ACGVJG_002353"
     CDS             complement(136..1302)
                     /locus_tag="ACGVJG_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6558267.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            1327..1653
                     /locus_tag="ACGVJG_002354"
     CDS             1327..1653
                     /locus_tag="ACGVJG_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558268.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDAGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            1806..2858
                     /locus_tag="ACGVJG_002355"
     CDS             1806..2858
                     /locus_tag="ACGVJG_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFJ6558269.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            2934..3152
                     /locus_tag="ACGVJG_002356"
     CDS             2934..3152
                     /locus_tag="ACGVJG_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558270.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            3139..5712
                     /locus_tag="ACGVJG_002357"
     CDS             3139..5712
                     /locus_tag="ACGVJG_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFJ6558271.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKF
                     DSNHETVIIDSEIRENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSC
                     N"
     gene            5703..5900
                     /locus_tag="ACGVJG_002358"
     CDS             5703..5900
                     /locus_tag="ACGVJG_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558272.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            5904..6515
                     /locus_tag="ACGVJG_002359"
     CDS             5904..6515
                     /locus_tag="ACGVJG_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFJ6558273.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            6500..7891
                     /locus_tag="ACGVJG_002360"
     CDS             6500..7891
                     /locus_tag="ACGVJG_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFJ6558274.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            8107..8421
                     /locus_tag="ACGVJG_002361"
     CDS             8107..8421
                     /locus_tag="ACGVJG_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558275.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            8414..8908
                     /locus_tag="ACGVJG_002362"
     CDS             8414..8908
                     /locus_tag="ACGVJG_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558276.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            8976..9290
                     /locus_tag="ACGVJG_002363"
     CDS             8976..9290
                     /locus_tag="ACGVJG_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558277.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            9389..>9598
                     /locus_tag="ACGVJG_002364"
     CDS             9389..>9598
                     /locus_tag="ACGVJG_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFJ6558278.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         3590 a         1314 c         1786 g         2908 t
ORIGIN      
        1 caggtcttaa gaaagcacgt aaagcttcac agttttcaaa acgttaatcg ttgttatacc
       61 aacgatttca gacactttct cattgcgaga aggtgtcttt ttttcgttct ggtaatgaga
      121 aaggtaatga caatattata aaccagtgta agcaatgaat ttttctaacg catttcgttg
      181 agctttttca gtaacatgag aataagtgtt aagggtagtt tcagcatttt tatgacccaa
      241 tcgatcttgt gtttctttga tacttgctcc accttctagt agaagagtag cgtgagtgtg
      301 ccggaatcca tgaatagtaa cgtgcttaaa tttatatttc ttctctaaac gtttcaatat
      361 attatttgaa tagtccgcat gcaaaggttg attaatgtca ccgtctgcat ttacataggt
      421 aaagataagt tgattttttg tatttgtatt tatgccaaac tgcaaaagct ctcgagcttg
      481 ttcttttttc cagattttta acatttcaat agtttgtgca tcaattggta atattcgttc
      541 ggaagatata ttttttgtat ttttttgaat cctgacagtt tttcttcttg cattcttctc
      601 ttcatttcga atagttgcat agctctttct aacatacatt agctgttctt caaaataaat
      661 gtcagaccaa tgaagagcag ctagctctcc tttacgtata ccagtagtaa tcaaaagctg
      721 gaagattaaa atatgtttta aaggctcttc tttattgaga atatcccaaa aagcttttag
      781 ttcttctaat gaaaagtaat tttcattttc tttcttttcc cgctttggta gatttgtatt
      841 tgccatagga tttcggtgta atagctctgc aaaaatggca tactcaaata tttgagagct
      901 gtaagactta agctgtttga tattcttgaa ttttaaagcc cattcattga ctgcattctg
      961 acagtgaaca ggagtaatct tagaaatgga cttattcccg aattttggta gtatatgcaa
     1021 gcggaaaagg tcttttgtac gttgatatgt cgtttcttgt acacgattcc tataagaagg
     1081 caaccattct tcatacactg ttataaacaa gtcattagaa ggcttatgat agccggtatc
     1141 ttcaatctcg accttttttc ttgcttcgtg taacttggct tctttttcag ttttaaaccc
     1201 tttttttaca tagtattctt gctttccatt ttcatcttta cgaacataca agcggacact
     1261 atatcgtgca ccgctttttg ttgtgtactt ttgtattgac ataactaatc tctccaatct
     1321 gataacttgg cgagttggct gattgaagaa atagaaaatg agaggagaaa aataatggat
     1381 gcaggaatta cagtaatgtt agataaacaa caaacgaatc aattgaaaaa ttatgttttt
     1441 gaaatgacaa aagaggctat tgatcaagca agaattgatg ccggattaga aagaccattt
     1501 ctaaaaggaa aagagatggc taaatatttg aatgtatcat atacaacttt tttgaaattt
     1561 aaaagaatgg gattgccagt tattttatta gaaaaaatgg agttgttttc aaaagaagaa
     1621 tgcaaaaaat ggatattgag tcatcaaatt taaaaaacac ctcgtttttt aaatttgatg
     1681 aagtagaaaa aattaaaaag agtgttcccg tcagtttgca gatcggggga acactcaata
     1741 ataaagttct gagaaaactt tatttgaatc tattatagca aataaaatca gaagcaggtg
     1801 tgacaatgaa tgaaaaagaa gttgaaagct tagttgaaaa gcaaggctat tctaattctg
     1861 atatatatgc agaattatac tatcaatatc ctattttttt aaacgtaaat gaaaaatata
     1921 agaaaatgaa actaagttct aagttagttt atatgttact aaaagataga aatagacttt
     1981 cattgaaaaa taacttgtat gatgagaatc aaaaaatttt tttgatattt gaaatcaaaa
     2041 atctcgtaga aatttcggga atgtcaaaaa atacaatctt atcagcacta gatgaacttc
     2101 aaaaagttaa acttcttgtg aaaagaaaaa atggcttcaa taaaagtaaa atgcaaatgt
     2161 tcccaaattt ttattatttg ttaaagcctg ttctgtgcaa agatgatgtt tataaaatta
     2221 ataagataac ccatcaagaa aatagacaaa aaaacttgat agttcaaaat ttgaactatg
     2281 gaacaaatca atattctgtt gatagttcaa aatttgaact atgcgacaca tcaatgaaaa
     2341 caagagacaa aaacaatttt gctgatagtt caaaaaatga actagaatta tataagtctt
     2401 ataaagatag taaagatatt aaagaatcac ataatgatga tcttcttgaa tcactttcaa
     2461 ctaaaaataa taaattagat gaagaattga tcgaaacatt tattgaagaa aatcttctta
     2521 aacaaatatg gggagaaagt attgtgaatt gcttaaaact ggctagcaat agtgattatc
     2581 gtcaattcga caactggtac aaaaaattca aatatgcgat tagatctgct gaaaaagaac
     2641 aaaaaattca actgaaaatt atctatgaaa tttgtaataa caagtatttt gtagatcatg
     2701 ttagagaaca gctatcaatg actttacgtg acttgatacg aagagcaaaa acagatcatc
     2761 gtatcaaaca aaaagacgat tatatctttg cttctctaaa aaataaagcg ttagaactaa
     2821 ttgaattgca aatcagcgaa ggaatagata agcaataaga atagaattga gtcaaatgaa
     2881 atggaggggt atctccactt catttgagaa ctttgaatat aggaggggtt attttgatta
     2941 aaaatcgttt aatagatcaa gtttggtgtc tagatcaaaa tacagatagt ctcgaattaa
     3001 tcgagactat ttgcttcaac actatagttc tcgatttaag agctgaaaat gataattgtg
     3061 taactcatgt cataattaat caaaaaatgg ctcagaagct ctctgagagc ctcagaaaat
     3121 gggctaaggg aggtaacgat gaaaataatt gaaaaaaaga ttgccaataa aaaaataaat
     3181 tggttggttg ttaattttct tgttctgata gctatgatta tttttacaag taatattgct
     3241 catgcgagta ttttcaattg gtttgatgac acaaaaagtc aaacagagtt tttgaataat
     3301 tctctttata gaccttacct aaaaaagtac aatggagatt tccttagtca gttcgggatt
     3361 tggctcggat gggctgtggt aaaaggcatg tttactgtga cagattcgat tcaaaatatg
     3421 attccagatg tgctggattt gttcaatttc attgaaagta cagggctaaa taatgtttat
     3481 caatcagccg taaatacaat tgtcattgga ttaatgatcc tttcattgat gtttgttggt
     3541 tacaaaatga ttactggaaa aggaacagtg gatttaaaat cggttggaat gaacgtagtg
     3601 atgtctatag cattaatact tttaatgcca accatgattt ctagtggtat tcaatttgct
     3661 aaaacatttt ataaggactc tactacgatt acaaattcag atgacggagt cgcttggtca
     3721 ttgataaaac aaggagtaac agatttagca tatattaata aaacagatca gtactcaagc
     3781 attgataaaa cggaggatcg aaataattta acacgaaaaa attttcaaca gacggatctc
     3841 acacaagtgt taacggataa agtgattgat aaattggaaa aagaaaatcc aattgcggac
     3901 aatcttcgtt atgaattagt tgaaaattct aataatgaat ttgttgctac aaagttttct
     3961 gacaacttct tatcaacatt ttctgatagc ttgaaatcag gctattaccg ataccaagca
     4021 aatctatggg ggatttcaat tgggttgaca gcgttagcaa tcgcctatgt gttcagcgcg
     4081 tttgtgatca ttacagcaat actagaactt gcatttaaac gtgtgttagg tgttcttgtt
     4141 tttgcaacag atattgagac aggacagcgt tcaaaagttg ttctatcaga tattctacaa
     4201 tgttacttaa ctgttgggtt tcaaggattt gggttatcaa tgtttgctat gtttatcaat
     4261 tttctgaact caggacaagg tatctcaaca aatattttta taaaaacaat tgcttatatc
     4321 tgtgccgttt ttgttttaat caaaggcagt ggaacagtca tgagatactt tggtgttgat
     4381 attggactca aagaaggcta tgggcaactt gcttctgctt ttggaatggg cgcaatgctg
     4441 tttagaaaag gatcaaatgg atttaatcgt gctaaaggaa gcggagaagg agaagatcga
     4501 aaaccagaaa agaatttcgg cgaaactcta tcaaaaaaag ctggcaaaac tggaagagct
     4561 ttgggatatg cccatgaacg tggattatcc ggtttagctt cagatggagc tacaatggct
     4621 agtgaaagag caacaaaacc atttaagagt atgcgtgata tagctaatga taccaagaat
     4681 aagtttaaag aaggtttaga tgatggtaca gtgtccgcta ttaataaaaa tagcaaacct
     4741 atgttagcta aaaataaaga agatgaaact ggaaaatatg ctgattccgt gccaacacgt
     4801 ctttcagata gaaaagatgg tgctaaagtt gaaaatgccg atagaattat gtctagttct
     4861 gaacgtatgc gtgaagcaat gaaaaataat gctgaatcga ataataatcc tgcaagcgca
     4921 attcagcaaa aagtccaaca agatattgaa gaacgtaaaa atgctatgca cggtcaagct
     4981 aagtctgctg aagaattaat taatcagaag cgtcatgaag cgaaatacac gcctgaagca
     5041 atgaatcgcg aggaaataat gttaaggaaa cgtgtagaag ggcggacagg cgctaatatt
     5101 gacggaaaag aagcgttgac caaagaaaga attcaagaag ctaaaaattc aaatacagga
     5161 ttagaaaaat tagtcaaaga aaatccacaa aattctacaa gtagtcatgg tggccgatca
     5221 gtagatgtcc gagaaaatat tcaaggttca ctgaatggtc atgttggacg gaatgttgat
     5281 gtaagagaga atttacaaag ttcttcaaat gctcaaggta ctaagacaat tgatgttcga
     5341 gaaaatctgc aaagtagcag tacaagtcaa ccaaaaacag tagatgttcg agaaaatgta
     5401 caaaattcta caagtagtca aggattcaaa acagtagatg ttcgggagaa cattcaaaaa
     5461 gatagtggag aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga cagaaagtca
     5521 gcgaaaaaat ttgattcaaa tcatgaaaca gtgattattg atagtgaaat ccgtgaaaat
     5581 gataagggat caaaatcaag acgaagattc acttatgaag acaatgagtt attcagagat
     5641 acacttaatg atcctaatcc acttttcgat aatttattaa gaaataaaag ggggggattg
     5701 tcatgcaatt aattctaaag attagtacgc atgatttctt agaacttgac gaattatgtg
     5761 aatcagtaaa gaacgcaaca aatgaaaaaa tcacggtcac taattatatt gaactgatga
     5821 ttcaagaaga attaaagatg aaaaatgaaa tagagagcaa tttcaaatgt tgctctctag
     5881 aaaaaaatgt gaggggataa tcaatggatg ataaacaaaa aatgtacgaa aaaaatcgca
     5941 aacatagtga ggccttaaat tttcgtgtcg ataaaaagac ttatcttgaa ctgacattga
     6001 ttgcaaaaca aaggggaatt tctagagcag acgtttgccg tgagcggtta aagggactca
     6061 aatatgaacc gccgttgtta gctgaagatg atagggtagt tttaaccaaa cagttaaatc
     6121 aaattggtaa caatttgaat caagcggtaa aggcattaaa tatcattgct aaatatttta
     6181 gagaagaatc tgaagataaa aaagaaaaga gcctacattt tagttttgat gctagccgtt
     6241 acaacatttt taaagatgaa cttgcgaata gcatagcaaa aaagttgatg attagttatc
     6301 cagatgacat ttttttggag tccaatcgga gtgccccctc tttttttaaa ttatcagaag
     6361 tagaaaaaga atatttcgaa tattggatag aaaagcaaac tagaccgggt acgaaagaag
     6421 agtattccca aaaagttgat cggaatttaa ttcactcaat tcagattatt gcaagtttaa
     6481 aagatgaggt gaaacaactt tgggagctac tataacatcg aaaggacgca agaaaaattt
     6541 atcctcgcta ttacaatatg cagctaatcc ttcaaaaaaa gaaaaatgtg ttgcagtctc
     6601 aggaataaat tgtagtgatg atagtgagga agcactgcaa gaaatgaatt tagtacgtac
     6661 caagatgaat aaatctaaag atatagcaca agcgtttcat gttattcatt cgtttgataa
     6721 gagtacttct aaagagttaa gtattgataa aatgcacgaa atagctgtcg aatttgcaga
     6781 gaaagctttt ccaaattcgc aaatcattgt tgcaagtcac aatgataaag atcattttca
     6841 tagtcatctt gtcattaata atattaacat ggaaacatgt aaaaggttgc gtattgaccc
     6901 taaagattta gaactaatgc gaaaaattaa cgatcaagtc ctcaatgaac atggtcttga
     6961 atcagtgaat aatgagtact atgaaaactt agaaaagaga accaatcttt attcaaacga
     7021 tccttctaag atgaaatatg gaaaaattaa ttatcaagag tttgtgcaaa atgcgatcga
     7081 agcaacgttg gaaaaatgcg aagtgaattc ttttgaagat ttttcggatc atttggcaaa
     7141 agaatataac attgaagttt atcaattttc aaaaacaaat aacaaacttg gctatgttct
     7201 ttacaaacaa ggtgtaaatt tctatgagcg tagaggtgag ttaattactt taggaagaga
     7261 taaacaatct agaaaagaaa aaagtcagta tgtcgaacgt acttttagtg caagaaagtt
     7321 agggaaaaga tattcatttg aatctgtttc tgaacaactt gaaaagaatt tagaagaatc
     7381 acatataaaa aattttttta atatggaaag agagcaagtt atgtcgctaa ttagtggagt
     7441 aaattacaaa actggtgagt taaatgtacc gtttgtggaa ttagattcca tcgcacaaaa
     7501 gcaatttagc gaccgtgaag gcgatgaaaa attcattaaa tatgatgact tcttgcgaga
     7561 ggtagacaag cattttgtaa atacaatgtc tttagaagat gatcaagaat acacgattaa
     7621 acttcatacg gatgtgaatc ctcaaacttt acgtagtgcg aaaagtaaaa caatttctat
     7681 taaaaagact gatcagaaag agttatttct accggcaaag ggttttgaaa attgttctca
     7741 tacaccttct gctaaacaga ttcactatca aatcagtcgc ataatgaatg acgatgtaaa
     7801 aatgtatcgt gctaaaaagg attatgaatt cacacaacaa atgattgaaa atcagcaaaa
     7861 aaggcaacaa aattttgaaa tggaattctg aatgggcttg ttagtggact ttccagtttt
     7921 ttgcctatga agagttgctt ggaaagaagc agtttttagg acatacgcca gctacccatc
     7981 tacttgccag tcaatccgcc ccgctcacgc taggctactt tctggcaagt cagatggcgg
     8041 gtaaatctct gagggagcaa gctccctaca gactccctcc caaaaaaaga aaggtggaag
     8101 aaaaaaatga actatccata ttttaaagtc tcggcttcag aagaaactaa agaaatattc
     8161 aacaattttt acaaccaaaa caaaggaatt tttggttcaa aggcaaatat gtttcgtgtt
     8221 atggtttcaa atttaccagt gttagctagt ccatcaaata ataaatttaa tgatcctgaa
     8281 agcattaaat ttgaacaaaa aatttcagaa ctagaatcaa tgataagtaa tgaagtaatt
     8341 gaaaagctgg atgacattga tcaaaagtta tcgtattctc taaaaaacaa atacaaaacg
     8401 gaggagaaaa aagatgttta aagtttactt cccgtttcag tggctcgtta aaggaattct
     8461 tttagtgcta ctaggattga ttctttttgg aagttatcta acaattaaag ccagacattt
     8521 tccagaagaa aagccaataa aaattgtaac aaaagattta aaaataggtg aaacttatca
     8581 ggcagatggg gttgaactaa catttaaaaa tattgaagtg accgaaaaaa gaaatgaatt
     8641 tgcacaacat gatcgtgtat tacaagtgac ctatacactt ataaatgata cagaaaatcc
     8701 gatacgtcca caacaatggt cgattgaaaa taaggatggt gaagaacttt atgagtatga
     8761 tatgatattg gataatccag agtatattga aagtggcaaa gaagaaacag taaattacta
     8821 ttatgaatta ccgattgaaa ggcaggacta ccaaatcgta gtacgtagag agaatggtta
     8881 catagttggg ttgccattta ctttataagt tttattttgt tagtagtttt taaggtaata
     8941 tcagttttgt gatttttgaa aatggaactg ggagaatggc tgaggaaaat aaagttgttc
     9001 cgtttgaaac attagtgata aataaggaag agtttgagca actttttctt gattttttta
     9061 ttcaatatga catgatttat gattttagtt cgtcactaga tttatttgaa ttagaagcag
     9121 aaataataga aaacagtcga gagaagaatg gtaaaatcat tatttcagta gaaaaattca
     9181 atgaattatt ctcacaaaca tttgaaatag atccagacta ttatcaacca caagaagttt
     9241 ttcgagagtt tagaaataag attattggtt ttagtcaaac tacaaaatga aagcttaata
     9301 aagcaagtgt tgatagaatt attcctcaac gcttgcttta ttttatgggc acttcataat
     9361 agaggtatag agaagagagg tattcatttt ggatactatt actccaacaa acttcaaaga
     9421 aaatataggt aaagtgttgc aagaagtagt tcattcaaat tctgaaatag aaattactag
     9481 ggattctaaa aatggattga acgatggcgt cattatgatt tctaagagaa aatgggagtt
     9541 gttacaagaa gaactttatt tacaacgaac gggtacatgg gcttatgtat ctgattta
//
LOCUS       DAYLZO010000105         9554 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.107, whole genome shotgun sequence.
ACCESSION   DAYLZO010000105 DAYLZO010000000
VERSION     DAYLZO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.107"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..641
                     /locus_tag="ACGVJG_002365"
     CDS             <1..641
                     /locus_tag="ACGVJG_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558279.1"
                     /translation="KSTVLSMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQS
                     NEINVQITVKELVEFGRFPYTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQL
                     QRAYIAMVLAQDTDYILLDEPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFA
                     ASYADEIVAMKDGQLFTQGTTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            664..1617
                     /locus_tag="ACGVJG_002366"
     CDS             664..1617
                     /locus_tag="ACGVJG_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFJ6558280.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(2261..2557)
                     /locus_tag="ACGVJG_002367"
     CDS             complement(2261..2557)
                     /locus_tag="ACGVJG_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFJ6558281.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            2912..3268
                     /locus_tag="ACGVJG_002368"
     CDS             2912..3268
                     /locus_tag="ACGVJG_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFJ6558282.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            3278..4177
                     /locus_tag="ACGVJG_002369"
     CDS             3278..4177
                     /locus_tag="ACGVJG_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFJ6558283.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            4697..5425
                     /locus_tag="ACGVJG_002370"
     CDS             4697..5425
                     /locus_tag="ACGVJG_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFJ6558284.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            5570..7597
                     /locus_tag="ACGVJG_002371"
     CDS             5570..7597
                     /locus_tag="ACGVJG_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFJ6558285.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            7705..8802
                     /locus_tag="ACGVJG_002372"
     CDS             7705..8802
                     /locus_tag="ACGVJG_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFJ6558286.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            9109..>9554
                     /locus_tag="ACGVJG_002373"
     CDS             9109..>9554
                     /locus_tag="ACGVJG_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558287.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         3332 a         1562 c         1891 g         2769 t
ORIGIN      
        1 gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca ggtgaaatct
       61 atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa aaattatcga
      121 tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta gtagaatttg
      181 gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc gttcaggaat
      241 cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact ctttcaggcg
      301 gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat tatattttac
      361 tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg cagattttaa
      421 aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat atcaactttg
      481 ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt acgcaaggaa
      541 cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg gacatacgga
      601 tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat taggagagaa
      661 gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt agcagcatgt
      721 ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca aactgaaact
      781 acattgacga taacagatag taatggagac caaatcgagg tgccaaataa tccagaaaaa
      841 gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt tggagataag
      901 gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa aaaagtagaa
      961 tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca gccagatcta
     1021 atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat tgctccgacc
     1081 attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa tgtcactgct
     1141 ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga attatctagt
     1201 gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact agtcacactt
     1261 gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt catccatgat
     1321 ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg tcaaagtgtt
     1381 tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga cagaacaaaa
     1441 gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt agcacaaacg
     1501 aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta tttaagcggg
     1561 ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt taaatagcat
     1621 aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac attcttaaat
     1681 agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt attgatgatt
     1741 ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca ttatctacat
     1801 catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat tttttcagca
     1861 tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga taaaacttaa
     1921 atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat ctgctgcttc
     1981 tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta
     2041 ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca tctgctgctt
     2101 ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc cagcgttctt
     2161 attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca atcagctgac
     2221 cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt gtctcagcaa
     2281 gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc ttactccacc
     2341 gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg cttaatatca
     2401 atccaaaact attcgttttt tttgaagcta attttttata aatatccaat gctttggttt
     2461 tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata ttttgtgttt
     2521 cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca aagaagtctt
     2581 ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa aaagtaattc
     2641 tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt atttaagaaa
     2701 ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc aagaaactga
     2761 acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa acgaatgact
     2821 tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc tgatgaataa
     2881 agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa aagaagcaca
     2941 agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata tcgaaaacgg
     3001 tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat ttcgattgat
     3061 cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg ctgatacacc
     3121 agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc catccacact
     3181 tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct tgagaaaagg
     3241 caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg cgatcatcag
     3301 cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc tttggcaagt
     3361 tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta ataaagtaga
     3421 ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga catttcattt
     3481 cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt ttccagaccc
     3541 acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt tagggatgaa
     3601 tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg tacgaatgaa
     3661 gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg aggtcaatgc
     3721 agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa aacttgaggt
     3781 gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat ctgctcctta
     3841 tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag aattacttga
     3901 ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct ttgaagataa
     3961 atccatcggt cacacgattt acagctgcag accttttggt tattattatg aatggcagct
     4021 gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta tgaaattacg
     4081 tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac atttgcaaga
     4141 ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta atgtgtaaaa
     4201 gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta tcgtggatat
     4261 gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga tttgtaactc
     4321 gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa aaaaaaatat
     4381 aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca gtcaagtcac
     4441 attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca gctgtatttc
     4501 tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc tgataatcat
     4561 ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt tcataagtat
     4621 cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag taaataaaga
     4681 aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga tgttattcag
     4741 catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg aatataacac
     4801 agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg ttgatccaga
     4861 gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg gaacaggagg
     4921 ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa tttctactgc
     4981 ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa aaccgacata
     5041 tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg tctctcaacc
     5101 agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga aatttacgaa
     5161 agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga attctgaatt
     5221 aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag gaacaggtgt
     5281 ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg cggtacagct
     5341 ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat tagtttggac
     5401 attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat aaatacgtag
     5461 tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta aatttgtttg
     5521 gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat tgaaaaagag
     5581 gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga tcatagggta
     5641 ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa gcaaactcac
     5701 ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg aggatttaag
     5761 tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg acaaacaagg
     5821 actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata ttatttctat
     5881 cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa atgggaagtt
     5941 actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa ataagaagat
     6001 tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc aacgagatag
     6061 agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta gtaatcccag
     6121 tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt ctggccaacc
     6181 ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg gttcttctga
     6241 tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg cttttgtcac
     6301 gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg tagctcctac
     6361 atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg agcaaagaag
     6421 tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg atctcattag
     6481 gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta ctttttttca
     6541 agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag ctgatgtaaa
     6601 agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata agataactat
     6661 cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc agactagtat
     6721 cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa atcaaattga
     6781 tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag attattttat
     6841 tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa ttcctgaaaa
     6901 agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc aatcgaatga
     6961 aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt ataaagataa
     7021 agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag gagagaagta
     7081 taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag cgaatacaga
     7141 aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag gcaaacttta
     7201 tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca gtagaccatt
     7261 aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg cttttggaag
     7321 ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg agcttccaga
     7381 agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg ctgttacgat
     7441 tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt ctacagctca
     7501 tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct ttgaggggaa
     7561 actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga atataacaaa
     7621 attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac agagacaaga
     7681 ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag accaaaacaa
     7741 caattattta taacactcct agttatttta ggggcacttt ttaacaatat atcagtgtca
     7801 gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat cgacaagaga
     7861 aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa agcggaattg
     7921 agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc aaccaccaat
     7981 tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag cctgatcttt
     8041 gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa aagtgtccaa
     8101 accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt ggctggtggg
     8161 atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga tcaaagctta
     8221 tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac actgaacgaa
     8281 gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc aagaaatgtc
     8341 atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt tattgaaaca
     8401 aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga aggattgcag
     8461 attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc gctaacccca
     8521 ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg aaaattttcg
     8581 agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga aaaagatttt
     8641 acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat taaaaaagac
     8701 aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt attatgcata
     8761 cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga taacaaatag
     8821 taaattttta ttattgtagg atggttgggc tctttttata acaatataaa atataccatt
     8881 ttattaataa aaatgcatac tagtaaatga tataattcta ataacaggtt tgatcgcgag
     8941 aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag acaggacgat gtatattata
     9001 caaacaagaa tcgctgacaa cagtgactcg gatccaggaa atcagtttta tccccagcta
     9061 gttggtgaat gcatggaaag taatatacgt agaaagagct gatgactaat gaattatgag
     9121 ttttttttaa gcaaagaaga tcacagaagt tattcattac ttaaatattt agaagcaagc
     9181 tcaaatttat cagagtcaat atcgaatgtt caagaagagt tgtcgctatc gacatttctc
     9241 ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa agttcgatct agagaaaaat
     9301 ttgcgagtaa ccgtcagtga attggatata tgtctggaaa ttaatggaaa atgttctagc
     9361 aaagctctgt tatctcgtta tataacggat tctttatcta tgaaaatggt attggctttc
     9421 tttcaggaaa aatatatctc agtagaaaat tttgctaaac agcatcacgt gagttattct
     9481 gtggcttata aagtattaca aagactaaaa agaaatctaa aaaaatatca aatttttttt
     9541 gaaaagagaa agtt
//
LOCUS       DAYLZO010000106         9518 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.108, whole genome shotgun sequence.
ACCESSION   DAYLZO010000106 DAYLZO010000000
VERSION     DAYLZO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.108"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(233..1120)
                     /locus_tag="ACGVJG_002374"
     CDS             complement(233..1120)
                     /locus_tag="ACGVJG_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6558288.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1117..1986)
                     /locus_tag="ACGVJG_002375"
     CDS             complement(1117..1986)
                     /locus_tag="ACGVJG_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6558289.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2123..3409)
                     /locus_tag="ACGVJG_002376"
     CDS             complement(2123..3409)
                     /locus_tag="ACGVJG_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6558290.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3682..4260
                     /locus_tag="ACGVJG_002377"
     CDS             3682..4260
                     /locus_tag="ACGVJG_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFJ6558291.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4260..5189
                     /locus_tag="ACGVJG_002378"
     CDS             4260..5189
                     /locus_tag="ACGVJG_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558292.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5186..6922
                     /locus_tag="ACGVJG_002379"
     CDS             5186..6922
                     /locus_tag="ACGVJG_002379"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFJ6558293.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6985..7821)
                     /gene="pheA"
                     /locus_tag="ACGVJG_002380"
     CDS             complement(6985..7821)
                     /gene="pheA"
                     /locus_tag="ACGVJG_002380"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFJ6558294.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7818..8327)
                     /locus_tag="ACGVJG_002381"
     CDS             complement(7818..8327)
                     /locus_tag="ACGVJG_002381"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFJ6558295.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8320..>9518)
                     /gene="aroA"
                     /locus_tag="ACGVJG_002382"
     CDS             complement(8320..>9518)
                     /gene="aroA"
                     /locus_tag="ACGVJG_002382"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFJ6558296.1"
                     /translation="EGTTTIQNFLRAEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSH
                     SFVQPTESIDMGNSGTTIRLLMGILAGQPFTTTLFGDASLSKRPMGRVMEPLREMGAD
                     LQGEENDQYLPITVTGTRSLSPIRYNMPVASAQVKSALLFAALQAEGTSVIVEKERSR
                     NHTEEMIRQFGGRITVEDKTIIVTGPQKLTGQQITVPGDISSAAFFLAAGLLVPESQL
                     LLKNVGVNPTRTGILDVLEEMGAAITQTNHNEQNQSADLSVKTSHLKKATIGGEIIPR
                     LIDELPILALVATQAEGITIIKDAEELKVKETNRIDAVAEELQKMGAKIKATADGLII
                     HGPTPLHGAKVSSRGDHRIGMMLQVAALIADGPCELEGAEAVSISYPAFFDDLAELVS
                     GGEAHG"
BASE COUNT         2863 a         1992 c         1707 g         2956 t
ORIGIN      
        1 taaaatgaca atcaggacag tcatcggcaa tatagcagca atcacttctt taaaattttt
       61 tagcatgtat cctcctcctt tactctttaa gtttacctta aaatgaaaga gttttcgagc
      121 acaaacttaa aacgttatca tttaattttt ctcaacataa aataacacac gtcccaaaag
      181 tatcttttta cttggaaaaa tgactttgaa ggaacgcgct tattgtgttt ttttatttta
      241 cagagccgat cattccttga acgaattgct tttgcaaaac aaaaaatacg atggcagttg
      301 gtaaagtagc gatgactaat ccagccatga tcattccata gtctggtgta taggaagcac
      361 ccatagcaga taaaacaagc ggaaccgtcc gtttatcagg agattgcaaa gctactaagg
      421 gccataaata attgttccag ctactcatga atgtgatgat tgcagccgcg gcataggtat
      481 ttttagccgt cggcatatac actctaaaaa aaatacttag ttcttttaga ccgtctaatc
      541 gagcggcttc tactagatct tttggaaaag atttaacatt ttgtcgaaag aagaagatca
      601 aaaaagcagt gctaacagat ggcaggatga caaccaagaa actgttgatt cccaaaaccg
      661 ttccgttcaa ttgtgaaaac attcgatata aagggatcat caatgccgca aaaggaacca
      721 tcatcgatag cagtagaata ttgaaaatta ggtcttttcg tttactttga taaatctcaa
      781 atccatatcc tgccattgac gaaatcagca atgcaaacac agtggtcacc aaagcgatca
      841 tcgctgaatt ccaaagggaa cgagtaaaat tcagctcatt ggaaaacaaa ttttgaaaat
      901 tgatgatcaa gttactgcca atctttattt ttcccgatgt gatatccacg ggtgtattcg
      961 tcattcccaa aaccatccag acaaaaggga aaatggaaat aaatgatagg atgcttaatg
     1021 ctgtatactt taatactgac ccaattgtcg aacgaagttc taatgcttgt tttttgacgt
     1081 tggggcgttc gattgtttct ttttctacca tatttttcat cgactttctc ctcctacttt
     1141 cgtttggatt atcgacaata tcacaataaa ggaaacaata acaaaggaaa ctgctgccgc
     1201 atatccaaaa ttaggcgtga atttataact caaattatag atgtattgag atagggtggt
     1261 cgttgcattt gctggtcccc cgccagtaat attttgtact tcatcaaaaa gctgcaatgt
     1321 cccgatcgtc gaagtaatgg aagtaaacaa aatgattggt tttaaatttg ggatcgtcac
     1381 tttgataaac tgctgccatt tagaagcacc gtcaatagat gctgcttcat aaatctccga
     1441 atcaatattt tgcatgcctg aaaggaaaaa aatcatattg tatccagtcc aacgccaagt
     1501 gatcgaaaga atgatcagaa ctctcgccca aattggatga gtcaaccagc caatcggtgt
     1561 gtcaatcata ccaatgccca acaacaagtt gtttacaagg ccagattgag aaaatagact
     1621 tttcatgatc agagaataac tgaccatcga agtgatacat ggaagaaaga tagcggttcg
     1681 aaacaaccct ttatatttca actttttatc atttaaaagg ttggaaataa aaagtgctag
     1741 aaacaacatg atcggtactt gtacaagtaa atacaggaat gtattaaaaa aagctttttt
     1801 aaaagttgca tctgtcaaca tccgctgata attacctaac ccgttaaatg tcatattgac
     1861 agggggacca ctttgaaaag acgtgatcaa agccaaaacc atcggaataa aaaccatgat
     1921 tgatatcaaa atgactggca agattaaaaa agcatgcccg tttttgaata aatctttctg
     1981 cttcattctt ttccctccaa ttctatcatt gattatctac atgaaaagac aagaaagaca
     2041 atatctgtaa ggcagacgtg atcttttttg tcttttcgtt tgtctgatga aaaagcgaag
     2101 aaattcactt ttttaccacc gatcaattag ccaattgtgc ttctacttgt ttttgtgtat
     2161 ctgctagtac cttgtctgtc tcttctccat tgatgattcg atgaagagct tcagctacga
     2221 ctgcttcaat tgcataggtc tcatgtccgt aattcacttc tggtatttca gatgtccatg
     2281 ttgaaaaatc gtcaaagatc ttttgaccac tgtaaaattc agaagcttct tgataagctt
     2341 cttgttcatt cgcgctaagc atcgtagata ctagtccgat ttctttcgca agagtgccca
     2401 tcagttcttc attcgaagca aacgttgctt ctaggaaatc tttcgctgaa tcttctccag
     2461 ctactccttt gatcacatac cagccagcgc cgccaaggtt agaagcttgc gctttttgca
     2521 gattttctgg aagtgcaggt attggtgcaa tcttccattt tccagattgg ttttctgctc
     2581 cttggatcgt tgaagaatac caagcgccag ttgggctaga agctactgct cctgattgta
     2641 tcgcagttac tccagcattc cagtcagagg tttgttcgac tagatcttct ttcaaaagag
     2701 tggcaaacaa ttcaagacca tactttaaag atgcattgtc ttggatcgtg actgtctccc
     2761 catcttcaga tgtataccat tctcctgcct gttgcataat catccttact cgaccaaggt
     2821 cagaaggatt gacttcagcg attttctttc ccgtcttttc tttgacatct cgcgctactt
     2881 gaatatactc gtcccacttc agattctcca tatcctcttc tgtataacct gcttctgcca
     2941 tcagatccgt acgatagaag tttgctgtta cgcccgaatc gaaaggaaca ccgtatattt
     3001 tatctcctac tttgtttacc gcaaatttat acggtgcaaa atgatcttcc tttacaatag
     3061 cagacaaatt agcgaatgca tctggataag aagtcaaata gccttgtatc ctataatctt
     3121 caatcaagac gatattgggc aatccttctg tattcccgct cgctaaagcg gtgttcagtt
     3181 tctgaacgat atcatcctga gacatcgtaa caacttctat ctctgtttct tcgttctcat
     3241 aaactttttt tgcttcattc actgctttga tattgaatgt ctcatcccac gcccagacag
     3301 tgactttttc ttctttgtct gctgtactac tagcacttcc gcatcctgtt aagaaacctg
     3361 atgctgccgc taaacaaacg actccagcaa ttaactttct gatgttcatt tttatccgct
     3421 ccttttccat ccattagttg tttacgtttg ctacattcat cataccaaaa tttgaaaacg
     3481 gtttctttat tattcagata cttcaaaaat ttctcctaaa ataataaaaa aatacaaatc
     3541 aactatgaca aaaggaataa tcttagcatc tagaccaaag atcgaaatgg tgaggtttct
     3601 ttaaaataaa aatatccttg cattttctcc tagaaataag tatttttaaa ttacaagact
     3661 attcttaagg cgggtaaaaa aatgccaata cgcgtaattc ttatcgatga tgaacagacg
     3721 attttagatg gaatgaaaca gttgatcaat tggtctgagc acgggtttca attaataggg
     3781 actttttcca gtccgctgaa agcgcttgat ttttctttgg tacatgcaat cgatatcgtg
     3841 gtcacagatg taatgatgcc tgaattaaat ggaatcgaat tatcgcggct attaaaacag
     3901 caacaacctg aaacacagat ccttatcctc tctagctatg atgaatttga tatggttaaa
     3961 gattctttta aagaaggcgt cgctgattat cttttaaagc ctcgattgag tcctgatttt
     4021 tttctaaatt ccttaactgc cgtttcacaa aaaatcaaaa aaaagcccaa caaaagccca
     4081 atcaccaatc gttatgagca gatctgcgag aatctttcac agcagattgc tggacaaact
     4141 cgtccattgc ttgaccctct tctttttgaa cacaccttgt ttttctgtta tataccgaaa
     4201 aacggccatt cagttcaaaa caaggctcta aacaaaaaca gtcgatcacc cattcattga
     4261 tggatagcga cgattcgttt actatttatc ctgtttctac cttttcggaa gaatccggct
     4321 atgtaatcaa tactgattcc aaagatacac ttaggaagtt tgttcaaaag tttcgtcaag
     4381 aagtttttcc aggtgatttt tttgttgttt ctgattcttt tgcaatccaa gatatcaatg
     4441 aaatattcca agagatcaga aattgcacca aaggacaacg tttctatcat aagggaaaga
     4501 accttgctta tcagcatgaa cttcttccgt tattaaccaa ccacgagcag caagcaagta
     4561 gttatttaac aaaaatcgtt cataaagact acgctttagc tgtcaaagaa cttgagaatt
     4621 tcttcacgac gatattggaa cagcctattg atccagctta tttgaaacat gaggcaatca
     4681 atcgtttcta tgcattactc aacgctttgg ctgacgagca tgttgggaat gaagaacttt
     4741 ctttgctcaa gatcgccctt ccttctttat tagccgatgc tgagtatttc gaagattttt
     4801 ctattcttgt caaagaaaca cttgagaaac tcacccactt ttctgcaaat gcaaaaaaag
     4861 acaatttcga tatcttgact ttgatctacg agttcgtcca ggaaaattat gagaaagaaa
     4921 tcaatttaca acttttatcc gaaaaatatc atttttcata tagctatctc tcagccatct
     4981 ttactgaaaa atttggtatc aacttttcaa aatatctaaa aaaagtccgt atcagtaaag
     5041 ccaagctctt tttgacggaa acacggctta ctttgacgga aatctgtgag aaagtcggct
     5101 ataccgaact gggttatttc tccagagttt ttaaagaaga gacaggaatg acaccttctc
     5161 aataccgtaa aggaactttt gtaaaatgag aaaaaaaatc aaagagcatg aactctttgc
     5221 caaacttctg ttcattttgt tcatcggtct ttttctgcaa gccatcgtca tttctttgtt
     5281 tatttatcat cgatcaaggg atgcctatat ccagctgttc aatcaatcga atgatgtggt
     5341 actaaaaaag attcaatctg aatttgaaag tttgaatgat acaatcgaga atacattagc
     5401 cgcttttgat tctaatccag ctgtcaaaag ctatttttcc aatgatccag cccaacatat
     5461 ggaacagctt cagcaactaa gaacaatcca aaaaatgaat gactctcttt caaaaatcca
     5521 tccaatgatc gattacgatg tattgatctt tggagaaaat ggaagaactt ttgtcggaaa
     5581 cgacatgctc actgccgtat ctgccgattc tttttttcaa tctgcgattg ctcagagagt
     5641 gaatgaacgt gctgctgata cacaaatgct ttttgcacac cacggattga ctcttcgcga
     5701 taagaaagca cccagcgtct tctttgtcaa aaaactgaaa aatacattga atcacgttta
     5761 cggatatgct gttttatcga tctcatccaa gcaactagcg aatctttttc aatctgtagt
     5821 aaatcctgag atatcgaaga tctccttcat gaacgaagaa cagacgatca tcgcatcaaa
     5881 tgaagaaaat acaattggga gccgatcaat gattttcaaa aaactaacag taggtgaaac
     5941 aacgatttca aatggacagc agttgacccg tcttcctctg taccgccagg attgtacatt
     6001 actcagtcaa gtcgatcttc attctttatc taaccaaatg ggcgtgattt ttccgattat
     6061 tttgtataat atcgtgacac ttgcttttgc tggctcattg gtatttatgt atttggatcg
     6121 acacacaaaa tcgatctacc gcttgattga ttcgctaaaa cggctagagg aagatggctt
     6181 aaaagctact gttcctgtac aaggaagcca tgaagtccaa ttgctaggga atacgatcaa
     6241 tcaattgcta agtaatgctc agcatcatta tgaacagacg atccaagacg aaaagaagaa
     6301 acgttcgctt gaaatccaaa ctatgcaagc acagatacag ccccatttta tttataatac
     6361 gctgactagc ttgaaatttt tgatttggca gaaaaaaaca gatgaagcag ttcaaggaat
     6421 cgacagtttc attactttac taagaagtac gattgggaaa aaagaagaag tgattccagt
     6481 caaggaagaa atcaaaagcg tacaaagtta catcgatctt ttgagtatgc gatatggcga
     6541 aagaatctcg gctaaaataa tgattcctga tgaactgcag tcattatcta tgccgaatat
     6601 gatccttcaa ccaattatcg aaaacgccta tcttcatgct tttcacaata aaacgcaagg
     6661 attcatcacg gtgtacggca aaacaaaaaa tcagcttctt ttattcgaag tgattgataa
     6721 cggcgatggc ttcgatactg aaaataccaa acaaaaaaat gatcattttt caggaatcgg
     6781 catcagccat gtagatgagc ggatacgcct aatgtatggc gatgcatatg gcttaacgat
     6841 ccaatcagtg attgggacag gaacgactgt aacgatttgt ctaccgcttc ttcctaaaaa
     6901 tcaatcagaa tatcaaaaat aaagaatagc caagcgctga tttttttatt ttaagttaaa
     6961 taaatcaatc agtattcttg actttcatct acgcgtctct tctactgtca gcactggata
     7021 acagcccaat atttgacttt ctcctccgag catcttgatt tcttggagcg cattctctac
     7081 taatgtcatt ggacggtcca acaacaaatc gatcacgaaa aaatattctc ccaagctagt
     7141 tttcaatggt cgggattcaa tcttacttaa gttgatttcc cgccagccaa aagctgccag
     7201 cattttatga agcatccctg gccgattagc aggtagtgtc aagatgacac tcatttttac
     7261 tggagctgac tgctggctgg tcatctttcg gtcaccgacg atccaaaaac gtgtctggtt
     7321 caactcatta tcttggatat tctcagctaa gatctccaat ccaacatgct gtgctgtttc
     7381 taatgaagca atcgctgctg cgtcttcttt cggatgttct gctacataca ttgctgcagc
     7441 agttgttgat tctgttgcta ctaaaggaac attaggataa tgcgtttcta aaaactgttg
     7501 tgattgtgct agtgcttgtg gatgtgaaag gatttttgta atctttgttg ctggattgcc
     7561 taaaagctgc tgcttgatag gaagaacgat ttcactttta acttcgactt ctggatgttt
     7621 ggaaagaaga tcgattgtgt gatgaacagc tccttccaat gaattctcta caggaacgac
     7681 agccagatcg acttgtttgc gaaacaaagc gcgcaaacaa acagggatgg aagcatacgc
     7741 agtcaattgt tctgtaggaa atagctgaca agccgcctga taagtgaatg aactttctgg
     7801 ccctagatag ctgacgttca tatgcttttg atcctttcta taatggtatc gatgatcttt
     7861 tctggtgttt gattggtcgt gttgattcga tgtgtcgcac tttcttcata ccaagaaagt
     7921 cgttgtgcca ataatgcttt tatttcttct cttgtttttc caagagctaa aggtcgaata
     7981 gacgtttcat cttgttggat acgatcaact agacagtccg cttctgcctc taagtaaaga
     8041 acaatcggct gtgtttttaa aaaacgccga ttttctgcac tttggacaat ccctccacca
     8101 gtcgcgataa ttttgtcttc atttgacagc tttcttaaaa tatctgactc cacttttcga
     8161 aagctttttt caccgtaata gtgaaaatat tcggcaatcg acaattggat atgttcctca
     8221 atctttgtat ctaaatctac atactctttt tgtaaacatt gagctaaccc tttaccgatt
     8281 gttgtttttc cagcgcccat gaaaccaatc agcaaaatgc tagccatgag cttctcctcc
     8341 tgacacaagt tctgctaaat cgtcaaaaaa tgccggataa gagattgaaa cagcttcagc
     8401 tccttctagt tcgcaaggtc catcagctat caaagcagct acttgcagca tcataccgat
     8461 tcgatgatcg cctctgctgg acacttttgc gccatgcaga ggtgtcggcc catggatgat
     8521 cagaccgtct gctgtcgctt ttatctttgc tcccattttc tgtaattctt ctgccaccgc
     8581 atcaattctg ttggtttctt ttacttttaa ttcttctgcg tctttgatga tcgtgattcc
     8641 ttctgcttga gtagccacta aagctagaat gggcaattca tcaatcagac gcggaatgat
     8701 ttctccacca atcgttgctt tcttcaaatg actcgtcttg acacttaaat cagccgattg
     8761 gttctgttca ttgtgattcg tctgggtaat cgctgcgccc atctcctcca acacatctaa
     8821 gataccagtt cttgttggat tgaccccgac attttttaac aacagttggc tttccggaac
     8881 aagaagtcct gctgctagaa agaaagcagc tgatgaaata tcacctggaa cagttatctg
     8941 ttgaccagtt aatttttgcg gtccggtcac tatgatcgtt ttatcttcca ctgtgatcct
     9001 tccgccaaat tgacgaatca tttcttccgt atggttacgg gaacgttctt tttcaacgat
     9061 tacggatgtg ccttctgctt gtaatgccgc aaacagcaaa gcagatttga cttgcgcact
     9121 ggctacaggc atattgtatc ggataggtga taaagagcgg gttcctgtca cagtgattgg
     9181 tagatactgg tcattttctt ctccttgcaa gtcagcgccc atctcgcgta aaggttccat
     9241 cactcgcccc attggacgtt tagacaacga agcatccccg aacagagtag tcgtaaaagg
     9301 ctgtccagcc aaaataccca tcaataaacg tatggtcgta ccggagtttc ccatgtcaat
     9361 tgattcggtt ggttggacaa atgagtggct accgcgacca tgaatcttga tcacctcttc
     9421 ctcttcttcg atctcgacac ctaattgttg gaaggcatgt aaagtactca gacaatcttc
     9481 tgcgcgcaaa aaattttgta tagtcgtcgt tccttcag
//
LOCUS       DAYLZO010000107         9498 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.109, whole genome shotgun sequence.
ACCESSION   DAYLZO010000107 DAYLZO010000000
VERSION     DAYLZO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.109"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(181..576)
                     /locus_tag="ACGVJG_002383"
     CDS             complement(181..576)
                     /locus_tag="ACGVJG_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFJ6558297.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            complement(569..940)
                     /locus_tag="ACGVJG_002384"
     CDS             complement(569..940)
                     /locus_tag="ACGVJG_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558298.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            complement(940..1281)
                     /locus_tag="ACGVJG_002385"
     CDS             complement(940..1281)
                     /locus_tag="ACGVJG_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558299.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            complement(1293..1508)
                     /locus_tag="ACGVJG_002386"
     CDS             complement(1293..1508)
                     /locus_tag="ACGVJG_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558300.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            complement(1531..2421)
                     /locus_tag="ACGVJG_002387"
     CDS             complement(1531..2421)
                     /locus_tag="ACGVJG_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFJ6558301.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            complement(2436..3059)
                     /locus_tag="ACGVJG_002388"
     CDS             complement(2436..3059)
                     /locus_tag="ACGVJG_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFJ6558302.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            complement(3186..3503)
                     /locus_tag="ACGVJG_002389"
     CDS             complement(3186..3503)
                     /locus_tag="ACGVJG_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558303.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            complement(3505..4557)
                     /locus_tag="ACGVJG_002390"
     CDS             complement(3505..4557)
                     /locus_tag="ACGVJG_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFJ6558304.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            complement(4475..6004)
                     /locus_tag="ACGVJG_002391"
     CDS             complement(4475..6004)
                     /locus_tag="ACGVJG_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFJ6558305.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            complement(6016..7431)
                     /locus_tag="ACGVJG_002392"
     CDS             complement(6016..7431)
                     /locus_tag="ACGVJG_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFJ6558306.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            complement(7424..8257)
                     /gene="terS"
                     /locus_tag="ACGVJG_002393"
     CDS             complement(7424..8257)
                     /gene="terS"
                     /locus_tag="ACGVJG_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFJ6558307.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            complement(8416..8646)
                     /locus_tag="ACGVJG_002394"
     CDS             complement(8416..8646)
                     /locus_tag="ACGVJG_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558308.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            complement(9393..>9498)
                     /locus_tag="ACGVJG_002395"
     CDS             complement(9393..>9498)
                     /locus_tag="ACGVJG_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFJ6558309.1"
                     /translation="VTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
BASE COUNT         2762 a         1903 c         1346 g         3487 t
ORIGIN      
        1 tcaattaaga aagaaacatt ttgaatatca attcctacat tttcagttcc agattccatt
       61 actgataaaa cgaaataaaa atctttagtt gactgcataa ctgaatcaag atatattgtc
      121 ccatcaggat agatttcttt taatttgcta ctgattgcag caatgatttc atctttcatg
      181 ttactttcct ttcttaacga tttttattgc catttgttta aatcttcgag gaatataaga
      241 catatttgca agattcgttg cacgagttaa catgaatttc cctttgacaa agccaccatt
      301 tttagtccta tgaccttctt ctacatattt aaaataatgt tcattgttta taacttctcc
      361 aacaattcga ccgcttgata gttttctagc ttttactaca cgatatcctc gccttaaatt
      421 acctgatttt acaggagtta atggtttagc taaactaacg actttattca ttgaatcgtt
      481 aacgaaggat attccttctt tttgagcaat tacagtcata tttttaaaat tctcaatgat
      541 cttttctgca tttgattcat atttgagatt acccattttt ctcacttcct atcaaattca
      601 cttcacaatg actaggatag taaaaaggct tagtagcaaa tagagaaaaa attaagccac
      661 tggccttttg agttattgtt attcgatctc ctttttttag tttaatctta ggatgaacaa
      721 ataatttata tgtgtcagtt gaaacattta ccatctctcc atcttctatg actgctagtc
      781 catcgatttg accttgtgaa agagcgcaag aaataggatt atcatatact tttttgtaat
      841 cttgaatagt aatatttgta ttagaatctt caatgtccgt cagtctttca ataatacaac
      901 tatcttcata agtagtttct aaaatatctg cttcattcat taaaaaaact ccaatcctcc
      961 acaaccaata actcttcgta tcaaatcacc atagcctaga agtaactcac taacatcatt
     1021 tgttgttgaa gcataactaa tcgttgtatc gccacgtcta actgaagata ctgttttttc
     1081 tgattcattt tttattattt tatacaacac ttcactaatc acgcttttta acttttccca
     1141 tgaaatatca tttttacaag tgttgtatga ttcaatttct agcaaaatta tttctaataa
     1201 attagttatg cgttcttcac tcaaatctgg aaaatcttct tttgtagact caatgatttc
     1261 cgtttttagc gattcatcca tcagatcact tcctaaattt cattaacatc aattgcatct
     1321 tccaaaattg caattgcttc tttatcatct atagaaatga taaattcgtt atttgaatct
     1381 gctgtgataa atctacgagt tttaggatga acaaacccaa caaaattttt agttttacct
     1441 actctatatt tcacgacttc atctttttta gtttcatctt tttttaccac tgtaattcct
     1501 cctatcaaaa aagggagtac taactccctt ttattcagac tttaaattta aaattgcccc
     1561 agagtttgaa gcattgtatt caagagagta ttcacctaca agaccgatac gtctagaatc
     1621 cgttgttttt gcaagttctt ctgctctcca ttcacgtaat ggacgtagtt tcacataatt
     1681 agtatcgatt gcggcaattg ttccttttgg caatgatggt tctagtagag caatacctgt
     1741 accgtaatta gacacgatat ttccaatttg caatccaaaa gttactcttt ccccgaactg
     1801 aacaattttt gttgattttc catctaactc atcagtcatt aactcctgca tatctggaga
     1861 aatcaagcat agtttttctc ccatataacc tttttcatac atcaatttaa ataaagtatc
     1921 gatatctttt cttgttactg cacccgcagt ggctgtttct gccttatttg ctgaattgat
     1981 caagttcaaa atcccattca ttcgacgacc tttagaacca ttttcatcag cttttacacc
     2041 agtaatcaat tttcggttca aatcaatttt catttccata ccacgtagtg ctacttgatt
     2101 agttaattca tttccgactc cattcacatt aatagcatct aatgtaccag atacagaggt
     2161 tgattttcta aaaatttcag tatagttgtt aaaccaagtt cgacctgatt cagcatctgg
     2221 atattctccg ccctcaagtt tttcagaaga atcatcgttg ttgatatcat attcacgcca
     2281 tttaatttct gtcgagtttg ctggctctgt ttttccagca ccaagtaaat agcttaaaaa
     2341 aggtgtattt ggtacttgca tagcattaat cgctggtgaa atatccaaat actctaaatt
     2401 atttaaagat gtttttttca taatttttca ctctcctagt taaattgttg aagaatttgt
     2461 cctaattgtt cttctggatt tgttaatgtt tcattgttct gctgaacctg gttattcgat
     2521 tgttgagttc caccaaatgc agacttcatt tgtagttctt ttaatgcatc tgcatgtttc
     2581 tcattaattg cattcaaaac acttgtaaaa ccttcaacag cattcttagt gaaatctgta
     2641 tccgaactaa caagattatt tagcataaat tgagaaatag actcttttaa ctctccatct
     2701 agttctaatc cagcaatttg ttctgcaaca aatgctttat tatcacttgt cacacgcaac
     2761 gctttttctg cttcaaattc agcttgtagt ttttccagtt gaatttgctc aggtgacttg
     2821 tttttcttag attcctcata ttccttgatg gtatcctgtt taattttatc taagttattt
     2881 tgtttccaag cttccaattg tttgtcggca acactttgag attgtgactg aataaatttt
     2941 tgagcttctt cattggattc cacaaaagat ttgaagtcat caaaagaaaa cttttgatcg
     3001 tcaccttcgg caaagtattg caaattcatt ggcattaatg atttgtgttt catgctcatt
     3061 ctcctttcgc cccacgattc gttttcacgc cccgcattgc tttgagtttt aatagttgcg
     3121 ccccaccatt caaccaagcc cagtattgcg ctagtttaac gtcatttcgg acaaaataaa
     3181 aaagcctaat tactagactt tccttctttg tagtcagttc taacaacaat acatcccatc
     3241 ttttcatacc aatcaactgt ttcttttaaa tttggtaatg aatgagataa taattgaatc
     3301 gttaaattaa cggtattctt atcaactgga gtattatctt gataattaac agtttgatcg
     3361 ccaataacca cgtaggcata attgccattc caatggtctc tcaagccatt aggatggttc
     3421 tcagactcaa tagctgtttt gtaggcttct gcaatatcag catttacatt aattgttagt
     3481 actgcttcaa aaaaatcttt cattctactt cctccgtttc aaattaattc caaaagaatc
     3541 atgtgcaaac tcatctaata aatcgccaaa taaacggtca tattcttcat caatatcctt
     3601 tcctaatttt ggtaaattag gaacatccgt acatctacaa cgactatgga aaggtgctcg
     3661 attttcacct atgacagcat ctttcagttt atagggattc ttacttgcct ttcctccaca
     3721 aatccggcaa actctctcat cttttgcagt caatacgttg tattcctcaa tacctgtttc
     3781 taaatatgat ttttcaatac catcttgtgc aaattttgca tactccgttc gaacgagatt
     3841 ttctatcgct ttgttatact ttgattcttc taacttaaac atgtcacaaa tttctgagtc
     3901 taatctcatg gtgtttaatg cgtgtataac gccttctcca ctagcaacac tttttacaat
     3961 agcattagag agtttctgtt caagcgatga aatatttccc caaagtcttc ttgaaaatgt
     4021 ctttcctgac catggatagt tcaaataatg tttaatttca ttttctggta aataatttgg
     4081 tatatcaacg tttaaaagtt gaacaaaaac attagcatta gaagaatatg ttctttgaag
     4141 aatttcctct aagcgatcag aaaaatattg attcacatct gcatccatcg catattctgc
     4201 aagaatttta aatacatctg atcgtatttg taataagcga ttgacttttg catagtcaaa
     4261 tggtggaaaa aattcatcga taaactcttt gtattttgca tcatacattt ttaatgactt
     4321 ataatttttt tccacatatt cacgatattt tttctgatta cttgtactgt aaaactccat
     4381 catttctgga taagtgatat tgtgtaagtc agcttgtgat aatagttttg cttgtatctc
     4441 tttcagtgct tcaggaaata cactagttaa ttttttaagt gtctgatttt cttgtttgag
     4501 ccttgcttga tcctccagtt ctcgacgttt ggtccaatac ttcgtttcta tcgtcactat
     4561 tgccacctcc gccaaaattg tattcactat ctggataagc acttccactt tccaattcca
     4621 tcatttcatt ttcataatca acatctgtta caaatggaat ttggctctga atagttcgtt
     4681 tggatacgta tggtgcaagt ttaggtaaag cttcagctaa ataacttaaa tcagttggca
     4741 aagatctaga gaaagtaaaa ataatttttt caggctcaat ttctactttg tcatgaaact
     4801 taagaaaagc agcaattgtt tctgcgcatt ctttcaatcc ttctctaaaa tattgctctt
     4861 tagtatttgt tttcgcttct aggctaatga tttgccactt gcgagcttct ccagaactgt
     4921 ttgatttaaa cacctcatcg ttaaaatcga ttgctttaca aatggtgtaa aactgttttt
     4981 tcaatagatc catatgatat tcattgaaat cttttgctaa attttttgtg acatattcag
     5041 cttttgcagt aggggctttt aagttgataa tccctagctg attcatcatt tctttagcag
     5101 tgcctttgct cattgttgtg ccagtaacaa gcatgtacgc aagcttaaat tgttcgattt
     5161 cgttttgttg atcagacaaa gcacgatcaa ttgcatctcc aatttcctca gctacttcaa
     5221 aatcacaata acgattcgta ttatttttaa attctgccaa ggtaatcgtc cccaaagggt
     5281 tatcggtctc atcaattttt ttgaacgttc cgcttgtaac aaaattcaaa tcaccatagc
     5341 aagcatatgt cagaatttta tttcttgtca taactttcat ttcttgaaaa aattttttct
     5401 ggtaagagtc atacttttct ttgatataga ttccggcatt tccatatctt tctgctctcc
     5461 aaggttcaat attacttgct cttaacttcc aaccatcatc ttcttccaca ggctctagca
     5521 atcggaaagc tactccacaa gctccttgaa aagttgcagt ttcagaatca agcattgcga
     5581 atctcatgga atcaagattg cttgttaatt tgttaaaagc ttctggaact gtcggaagtt
     5641 ctatatcatt ttttaagaat ttatctttta atctttgaat gagtgttctt ttttgttccg
     5701 atacatcgta atcccatttg ataggtatac cagtaaaatg attgactgct tggtctacga
     5761 caattgaata cataccagca tgaagtttat tattaacttt tacaatatca gtgttaggct
     5821 ttggtctatt atctatttca ttcttttcgc tagtataagc tagatatttt ctttcgcgat
     5881 cagcaaaaaa tggtttcata tcttctataa aaccattcgg atcaaacatt ccatcttcaa
     5941 tttgtgttgc atatttaaca cgtagcttct tatatctctc taaagataat actgtactaa
     6001 tcaaaagctc acctcctaaa attgaataaa ttgatagtga cttggtggct cataaaacgc
     6061 taaagccagt gcgtcagcaa tatcgggact accaatattt cgctttttca tatcatcttt
     6121 actttctaaa cgtatacgac ttctacttgt cattttgaat ttgcgagtac ttaattcttt
     6181 gattaacgaa ctatcagaag gcaattcaat aacaggctgt tctccgttaa gatttgctgt
     6241 catattttct tctaacattt ccttgatgtt tccccatagt tgagtaccta aattatcgta
     6301 aaaatcgtct tctgatgttg aaccgttatt cactccaaac acctcaaaag gataatgttt
     6361 gtcttctata agttcttcta ggcggtcggt tacaccacct ccgacaccag tgtcatcgac
     6421 tttaatcatc actttatcaa tactcggata ttgactcatt agattcttgg ccatgttgat
     6481 gacatatcct gttgtttcca tggtgctacg ttttgaatac ttctcatact ccaatgccct
     6541 ggtagcaatt ctaggaaaga gaatcgtaga atcatcacca tatcgagcta cgtcgacacc
     6601 aatatgagca acctttgttt tattgactaa agaatcacta atttgttttt ctgtagccaa
     6661 ttcaactgtt tcaagactga taaatgaatc caacgcacct ttcggaaatt ctccaaaaat
     6721 acggacacga gcaacatcac tttcttttcc atatttttta agaatcattt ctatattgtc
     6781 tttgtttgta cgtttactat catagcttga tactttatga actctgtatt tatcacggtc
     6841 tgaattatgg gaatcataaa agacaccttc aatattattg gggtttccac acatcaacag
     6901 cttattatca aaacctgata gcgtaccaag aatagcttcc ataattggat cagacacacc
     6961 agaagcttca tctaccacaa tcaacatatg gtcctcgtga aaaccttgca tactttctgg
     7021 tttagtcgct gttctagccg tagcaaacca acgctctgaa tcaccaacca tataaatttt
     7081 ggtctttgtc cacttcagta agtttttgat caagctatca tttaaccatt tagctacctc
     7141 tgcccaaagt acatcgtaaa gttgcttcat tgtcggagct gttgctatta ctttagcgta
     7201 gggtcgacac gttaagaacc aaagtattgc tcctgcttct aatgctgttt ttccaactcc
     7261 ttgaccagaa cgaacagaaa cctttgaaaa ttcagctaaa tcatttaaaa cattttcttg
     7321 ccattcatca ggattcaaat gcaaaatatc ttggcaaaaa gctactggtt tatcatagta
     7381 ataatcaatg gcagaaccaa tatcagcaaa aggaataaat tcattattca ttttctttca
     7441 ccgcccgttt gtttgcggca tttaaaactg cctgtttcca ttcttctatt tcttcacctg
     7501 aattatttcc gccaatttca tttgtctcag cttggatctt atcagtctga gctttaatta
     7561 aatcagttct gtattcattc atataaagtt cattcatttg cttgatggct ttagctaact
     7621 ggttgctaat ccgagtcaat gaatcttcaa ttgaaagaat atcatcaatt ttgcgatgtt
     7681 tttttctgct aatctgcaca tcttgcataa cctcacgctt gatttctagc tttttaccat
     7741 ttttttcaat cggagtttta atctttctta gctgctgcaa tcgttcgact tcttcatcat
     7801 ttagtccagc ttcagcttgt tggattcttt tcatcattcg aagttgtctt attttaagaa
     7861 gtcgtatttc ttcggacaaa gcaaaagaag gatcatcatt caaactagaa tagatgtcct
     7921 tctcattatc ggttaacgtg tcaaaaaata ttgtttcata ctcacctgtt ttcaaagcgt
     7981 ttttattacc cactggcgga gaagctcggc tatttccttt gttaccttta gcattttgat
     8041 taccaatagg agcgccacct tgattagtaa cgttactttt gccattggta acgttactaa
     8101 tcaattcagc gctccatttg tcttgcgatt tccattttct aacctgagaa tctgaaacat
     8161 ttaattcaga tgcaatttcc tttaactgct tttctccgtt ggattctaac caaattcttt
     8221 tggcttcatc acgtcttgga tcacgttgtc ttgccattca atacacacca cctcacattc
     8281 tgtttaggtt gagttttgtt ttctaatttt caatatcttt tagcattaaa tcagcttcaa
     8341 tcaatatctt taaatcggaa actttgtcta acttgatttg tcctgattgg agatttttaa
     8401 gccatttctt gcattctaat ccctcaaatt cttgttaata ttattttgaa tgtttggttc
     8461 atcatagaaa ccatgaccgc aataaacgag cttgcacgca tcaatctcac atggttctgc
     8521 ttctctcccc atttcgacaa tagagtactt ccctttgatt tgtacagaac gcacaacacg
     8581 cactgaacaa tcatcaatgg ttcgaggata ttcattagtt agcgatacat accagtagtt
     8641 tctcattatg tagcctcctt tatgtaaaga aaaagaccac tcaaagagtg atctaatatg
     8701 taatgcacag gcagggacgt ttccgatcct gtgcttgaga catttgacga ttcatttgta
     8761 ccgaatccca aatctcaatc taacctaacc agttatgtaa tagcaaccta cacgatgcac
     8821 aaaacgcgta cgaaattgcg cacccctata tttttaaacc gccgatgcct cggttgccaa
     8881 agtcactggc aaggaatcga accttgcatg gttgccgaag cattgaccta gcacacatgc
     8941 ttagcgtcta ccctttccgc cacagtgaca ctataaaatt attcttggct gctactattt
     9001 tttattttgc ccatttttaa atccaatcat atatacatta agacaaagcg caaaaattga
     9061 aattattaat ggaatcattt ctcttcactt acctttagtt atcgtgtgaa taattaaaaa
     9121 aacaatagac agcaacggat gatagataat aagaacaatt tagaaggagt tgaaattcac
     9181 atccttattc ttaatatttc cgttgctgcc tatcgaagct taattaaacg atgagggaga
     9241 tttcctccct tacattttat tttgtcgatc ctgtttccta atttttcgac actatcataa
     9301 tacaacgttg aataggtaag tgattggtat aaaaaaggta taaaatggaa accaaattgg
     9361 taataaaagg gtataaaaag tgtaaaaact ggctacttga aagcaaccag ttctaacgat
     9421 gaagcaaatt ggatgataat tctgtttgat tctactttaa ccgattcttc gctagtatta
     9481 ttcctttgag cagttaca
//
LOCUS       DAYLZO010000108         9308 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.110, whole genome shotgun sequence.
ACCESSION   DAYLZO010000108 DAYLZO010000000
VERSION     DAYLZO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.110"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..190)
                     /locus_tag="ACGVJG_002396"
     CDS             complement(<1..190)
                     /locus_tag="ACGVJG_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558310.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="ACGVJG_002397"
     CDS             complement(200..1015)
                     /locus_tag="ACGVJG_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFJ6558311.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="ACGVJG_002398"
     CDS             complement(1277..1720)
                     /locus_tag="ACGVJG_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFJ6558312.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="ACGVJG_002399"
     CDS             complement(1854..2192)
                     /locus_tag="ACGVJG_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFJ6558313.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="ACGVJG_002400"
     CDS             complement(2180..2557)
                     /locus_tag="ACGVJG_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFJ6558314.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="ACGVJG_002401"
     CDS             2781..3974
                     /locus_tag="ACGVJG_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFJ6558315.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="ACGVJG_002402"
     CDS             complement(4140..4562)
                     /locus_tag="ACGVJG_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558316.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="ACGVJG_002403"
     CDS             4957..5151
                     /locus_tag="ACGVJG_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558317.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5594
                     /locus_tag="ACGVJG_002404"
     CDS             5148..5594
                     /locus_tag="ACGVJG_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558318.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5804..6976)
                     /locus_tag="ACGVJG_002405"
     CDS             complement(5804..6976)
                     /locus_tag="ACGVJG_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFJ6558319.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="ACGVJG_002406"
     CDS             complement(7182..7997)
                     /locus_tag="ACGVJG_002406"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFJ6558320.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="ACGVJG_002407"
     CDS             complement(8147..8593)
                     /locus_tag="ACGVJG_002407"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFJ6558321.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..>9308)
                     /locus_tag="ACGVJG_002408"
     CDS             complement(8590..>9308)
                     /locus_tag="ACGVJG_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFJ6558322.1"
                     /translation="IKHGFVLSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVR
                     LTTDNQTVETHLAIQTGGKQFVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAK
                     DDSRATIFTSSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHP
                     IPLEIINEPKANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLS
                     TGTAWFYKKQQKGNNRREIK"
BASE COUNT         2689 a         1810 c         1666 g         3143 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag ctaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattt
//
LOCUS       DAYLZO010000109         9129 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.111, whole genome shotgun sequence.
ACCESSION   DAYLZO010000109 DAYLZO010000000
VERSION     DAYLZO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.111"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..263
                     /locus_tag="ACGVJG_002409"
     CDS             <1..263
                     /locus_tag="ACGVJG_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558323.1"
                     /translation="AQLWNMMYFNIGPKVHEELDFCFATDPIEKVKEVKILHNAGVTT
                     NDEDLFFKGRYVTSTPFDEDLSFVNKKKCSYAYVKAIKAVVR"
     gene            260..571
                     /locus_tag="ACGVJG_002410"
     CDS             260..571
                     /locus_tag="ACGVJG_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558324.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            561..914
                     /locus_tag="ACGVJG_002411"
     CDS             561..914
                     /locus_tag="ACGVJG_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFJ6558325.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            904..1305
                     /locus_tag="ACGVJG_002412"
     CDS             904..1305
                     /locus_tag="ACGVJG_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558326.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            1298..1702
                     /locus_tag="ACGVJG_002413"
     CDS             1298..1702
                     /locus_tag="ACGVJG_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558327.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            1714..2319
                     /locus_tag="ACGVJG_002414"
     CDS             1714..2319
                     /locus_tag="ACGVJG_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFJ6558328.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            2339..2701
                     /locus_tag="ACGVJG_002415"
     CDS             2339..2701
                     /locus_tag="ACGVJG_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558329.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            2704..2886
                     /locus_tag="ACGVJG_002416"
     CDS             2704..2886
                     /locus_tag="ACGVJG_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558330.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            2903..6322
                     /locus_tag="ACGVJG_002417"
     CDS             2903..6322
                     /locus_tag="ACGVJG_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFJ6558331.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            6373..7110
                     /locus_tag="ACGVJG_002418"
     CDS             6373..7110
                     /locus_tag="ACGVJG_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFJ6558332.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            7120..>9129
                     /locus_tag="ACGVJG_002419"
     CDS             7120..>9129
                     /locus_tag="ACGVJG_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFJ6558333.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADY"
BASE COUNT         3217 a         1349 c         1918 g         2645 t
ORIGIN      
        1 gggcacagct ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag
       61 acttttgttt tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg
      121 ctggagtaac aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc
      181 cttttgatga agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag
      241 caattaaggc ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt
      301 gaaggataat atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc
      361 taaaaatgct gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag
      421 cttagaatac ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga
      481 gtttgaacaa aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga
      541 atatattgca ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg
      601 gttggttaaa gattttgacg caaactacca aaagaaatga actaggaaaa aagattggtg
      661 tagaagatac agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata
      721 taacagctat ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc
      781 caatcgccaa gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca
      841 acgttatcta tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg
      901 aatatggtga ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc
      961 aaaacttcct tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat
     1021 gagctacctg atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact
     1081 gagccaaaat atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa
     1141 gatttaacag gagattcact agatattatt tcattgattc aaaatcgttc gattcgtttt
     1201 cagagaatgg atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg
     1261 gtatttacgt ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct
     1321 aaaaataaat ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga
     1381 acgactgatt aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac
     1441 agaaaaaatt cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag
     1501 tcgtccactt aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt
     1561 tgagtattta aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga
     1621 atttatgaga agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca
     1681 attcgataaa ttaaataaat aggaggaaca acaatggcta aaacaacaac agtaacaact
     1741 tttgacaata taagtattaa aagaatttct tttaatttta agaatgcaac aaatgcaatt
     1801 tcaactgatt gtaatggaca attagatggc gaaacagaaa tgcagagaat tgtaaaaaaa
     1861 tgcggttcaa cagaagtaaa atcgaaatct aaaccaatca atatgacggt aacaattact
     1921 gcacatgtac cgatggaagt ttatcgacgc ttcaatgggt tgaaacaaga tgaacgtatt
     1981 aaatcgggaa tttactctta tggtcctgat tccgtagggg aagatttctc tcttgctgca
     2041 gagatcgtgg atgatttcga agaaaaaaat aagctgattg gtatgttagc atgcacttcg
     2101 aatacaggat taacattctc tattgaaaat ggtgcggatg aagtagctgc gttagaacta
     2161 gaaacaaaag ttatgcaaga tgaatttggt aaattttatc atgaagcaat tgttgcagaa
     2221 cttgaagaag acttaacaga tcaatggatg acgaatctat ctgctgatgt gattaaaaaa
     2281 acttcagttg tgacaactac ggccactcaa tcacagtaaa aaaaacggag gtagcgaaat
     2341 gaacgaagat tactcaaaaa ttgaactaaa cgatggaaca attttgaatt tagaacctaa
     2401 actgaatatc aagaaattat tgatgatcaa tagagatttt aacacagacg agtttgcaaa
     2461 aatgactgtg ggaaaaggat ccatggatat ttctgttatt caaggtgcaa aggctgtgta
     2521 tattgcttac cgccaagcga acatgactga ttatatttca ttcgatgaat ttatcgataa
     2581 atgggatttt gatatggcta ctgccagcta tatttatcaa ttgatgatgt tcaaacaagc
     2641 acgcgatgct tatcaaaaag aatttgaaaa agcaaataag gaaaaaaagc ttcaaaagta
     2701 aaaatgccaa agctcttagt tgaaacgtgg gtcgatgtct attcgatgtt gaccgacgtt
     2761 ttttctatgc cttcagattt ggttttaagc gatatctgtt tagatgacat tttgcaaatg
     2821 gcttacaaca agagtgctta tgaaggatgg aaaaactatg caataaacca atcccagaaa
     2881 aactaaagaa aggaggtaaa aaatggctaa aaagagaaca gaagcagaag taacattcat
     2941 agctaacgat gacggattga aatctacgtt aaaagaaatc agtgctgaat taactaaaaa
     3001 tagagcagaa ttaaaactag aacaagctca attacaacag actggttctg aatcagacaa
     3061 gttaggaagt aaattatctt ctttagaaaa gcagtatgaa ttacaaagtc aaaaagttga
     3121 agtaactagc caacgtttag ccaatgccaa aaaatattat ggagaaaatt ccaccgaagt
     3181 tcagaaactt gagagagaac tgattaacca acaaacagcg caacaacgtt tgtcaaacga
     3241 aattgataaa acgagtaatg cactagctca agcaaaaggc gaaatacaga cgtacgagtc
     3301 tacaatgcaa cagttggata gtgaacaaaa aaatgttcaa gctagtgctt ctctgattga
     3361 atccgaatac aaaaaatggc aagcaactgc tggtcaatca gcttctgaat ccgagaaatt
     3421 agcgaaagcc caagaatatg tttctcaaca atctgaaaat gcagagaaaa cgatagatat
     3481 cctgagacga cagttagaag ctacacagtc tgagtttggc gctacatcca cagaagcaat
     3541 gcagatggag gcgaagctta atgatgctga acgtgaattt gaagagttag gacaagctgc
     3601 taaaaatgta gatacaacta acttggacga tatcggaagc aaaatagata tgaataatct
     3661 aatggaagct tctgacgttt taagcgacat tggcgataag cttacagaat tagggaaaca
     3721 agcagtggac tctgctaata gtgtaggtag ttcccagagt aaaatacaag ctaattttgg
     3781 tttgactaaa caagaggctg aagaattaac gaatgtagcc agagacattt attataaagg
     3841 ttttggagaa tcgttagatc agtccacaga tgcattgatt ttggtaaagc gtaatttagg
     3901 cgatttaaat aatcaagatt tacaaaatat cacggaacaa gctatggtcc tagaaaacac
     3961 catgggcgct gatatggatg aaacgttacg tggtgtaaat ggcttaatgg tcaatttcgg
     4021 cttgagcgct caagatgcaa tggatttaat ggtttcgggt actcaaaacg gtttagataa
     4081 aacgcacgaa ttaggcgaca atatggcaga atatagccaa ttatggagtc aaatgggata
     4141 ttcagctgat gaaacgttcg gaatgcttca aaatggttta gatgcgggtg cttataacct
     4201 tgataaagtc aatgacttag ttaaggaaat gggaatatcg ttaacagatg gtcgatttga
     4261 gcaaaacatg gatatgttta gtgaaagtac tagaaaagct tttgaagagt ggaaaaatgg
     4321 cggaggaaca caaaaagacg ttattaattc catgattcaa gattttagca atatggatgg
     4381 tcaatacgac caattaaata aagcttctac aatttggtct gcacttggcg aagataacgc
     4441 gatgaaagtt gtccaatctt tgactgatgt taaccataca tttgatgatg ttagtggatc
     4501 tgcacaaaaa atgaatgaag attctactac tccgttacaa gaattaaacg gaaaaatagc
     4561 tgaattaaag gattcattag ctcctatagg caacacaatc atagatgcac tcgaaccagt
     4621 aattgatttt ctaggaaaga tggctgatgc gtttaataat cttccacaac cagtacagga
     4681 ttatgccgta gcgattggcg gattgactgc tgcatttact ttattaatgc caataatagt
     4741 tggcttcatg gctctaggtg gtcctactac attaataata ggagcagtta ttactgttat
     4801 tgctggagtt atagcaatta taaaaaactg gggcgcaatt actgactggt ttaagggaat
     4861 atggagtaaa ttcactgatt ggttgggtgg tacttgggaa agtataaaag aaggtgcctc
     4921 atcagtttgg gatggagtta aagaaacctg gtctggattt gtagattggg ttcaagatat
     4981 ttggcaagga gtttctgatt ggtttggaga gctatggagc ggattagttg aaggagcttc
     5041 caacatctgg caaggagtcc aagagacttg gcaagcattc gttgattggg tttcaaatat
     5101 ttggaacgga gtcaaagaag tatggtcgat tatttgggca gacattgtag gaattgttca
     5161 aataccatgg accttaataa cgtcattgat tcaagccggt attaatatta tcgtgggtat
     5221 ttttgatgta gctggacagt tattaggcgc agcttggcaa gctgtttgga cacctatttc
     5281 tgatttcctt aaaaacactt gggatactat gacacaatgg gtaagcatcg cttggaatgg
     5341 aattgtaact acattccata ctatatttga tccagtagtg gcatggtgga atggtatatg
     5401 gacagctatt agtactacgg cttcaaatat ttggaattca attagtgcaa cagcttctag
     5461 tatttggaac agtatcaaga atacaatcac tagcttggta caagcagctg ctacagtaat
     5521 tcaaaatatt tggtcaactg tatctagttg gttaggtgga atttggaatt caatcagctc
     5581 tacagcatca aatatctgga atagcgtgac tagtagtata agcaatgcta taaacgcagc
     5641 taaaagtgcc attcaaagtg tttggaatag tatatcttcg tggatcagcg gaatttggaa
     5701 cggtatcaaa aacactgctt tgaatctttg gaatggaatt acaagcacta ttagctctaa
     5761 agtaaacgat ggaaaaaatg caatttcaag cggttggtcc aatctaacag gtattgtttc
     5821 cgacatattc aataatgtta aaagtacaat tgctaacatt tgggaaggta tcaaaaagac
     5881 tgttagcgct ccaattgatt ggatcagaga taaaatcagt agtatctttg ataatttgaa
     5941 tatttctata ccacatattc cgttaccaca ttttaaattg agcggagaat tcaatccatt
     6001 gaaggggaaa atcccaacgt tgggtgttga ttggtatgcg aaaggtagtg tgtttaattc
     6061 tccgaatatt atcggtgtcg gcgaagcagg acctgaagca gttttacctt tgaaaagatc
     6121 tgtgctgcaa gaaattggtg atcgtatctt gagtagcaca tcagtttcat ctagggcaca
     6181 aacgattcaa cctgtgaata actacgaatt caatttcaca attgatggta acgcagatga
     6241 ggttactatg aagcaaacaa ctcaacaaat cattgatagc attacaaaag ttcaaaatga
     6301 taatgcttcg gcatggcgtt aaacaggaga gtatttctcc tgttttttta gtattaaaaa
     6361 ggatgtgaaa aaatgactga ttgtatacat tctataatcg atggatttcc tgattatttg
     6421 cataaattgg ctttagctga aagaccaacc ataccttctc caaaaagaca gagagttgaa
     6481 acttctgttt taggaaggtt aggtggctta gtacaagatt actcgtttga agacatgtcg
     6541 tttacattgc actataacta tttagaggat gtggaagacc atcaagcgtt caagcaatcg
     6601 ttttatatca tgcgtcattg gttaaattat gcaaagaaat tagaattctc tgatgatccc
     6661 aacgtctatt atgttatcca gactatcgat attggggatg cagaaaacga tattgttgaa
     6721 tggggagagt tcgatgtaaa tattactgcg aaaccattcg caagagttca agaagatgta
     6781 cctataaccg tagataaacc acagtcattt aacttgctga ataatagttt agaagaaagt
     6841 tttccaaaga ttatcatcac tccttcagct acttcatgcc agttcatctt aaatgattat
     6901 gtgtttagtt ttgaaggctt agtagtagga actgacgtag tcattgatag tgatttgatg
     6961 ctttgctacg aagagcaatc ggacggagat attttagatc ggtccaacaa aatgaagacc
     7021 atgcaatatc cgacattgca agtggatatt aattatttta attgtactgg tttgagcaaa
     7081 atacaaattt atcgtaatgg gttaaggtag gtgaaataga tgatcgataa tttaataact
     7141 atttacgata aaaacgacgc gaataattta gctgaacatt tatatgatac gcaaggttta
     7201 ggcgctttgt cagactggtt aacagctact gttagcaata aattaaacgg agccgagata
     7261 tttcagggta cttatccaat aagcggaact aatgcagatt tgattgtaga aggacgtatt
     7321 attcagtgtt atgtagatga aaatcgagca aaacagcgtc tacggattta ttatgcaaag
     7381 acttctgtaa taggcaatac gatagaagta aaagctgaac ctattttcaa tgatataaga
     7441 aaatcggtgt tgaataaata tgacagtgga acagaaaaga tcactgctag tcaggcatgg
     7501 caaaacgcaa aaactttagc gaaaccagtt attccttcac agttttcttt ctcgtcatta
     7561 gtagatacgc ttgctaatgt gaagatagaa aaggcgaatt ttttagaatt ctttggtgga
     7621 aaagagggat ctattctaga tcgatttcat ggggaatttc taaaagataa taacacatta
     7681 cgtcatgaaa aaaggctagg cacggatcat aaaatcaaag cgatttatac taaaaactta
     7741 actggtcttg acttagagat tgatgctcaa agtgttttag ttggagttta tccattcatt
     7801 agcagctctt cagaaggaga agacgagatc actctaccag aagaagttat tttcacggat
     7861 tacgtggatg attatcctgc tggatatgtt tcttttgttg attttaaaga caaagcgact
     7921 gatgtagcca cattaaggga agctgctaaa gactggttga aaacaaacat agataaacaa
     7981 aaaccacaag tgagtggttc gattgaatta gtaccattga ggcatcaaag aggctatgaa
     8041 aaatttgttg atctagaaaa agtttcgatg ggtgacggag tagatgtgta tcatccacag
     8101 ttaaaagtga atatgtcagc gagaattgtg gaatatacgt ttaatgttct aaccaattca
     8161 tacgataaat tagttgtagg aaacgtcaaa acaaacttct tagaaaacac agagaataat
     8221 gtcagcaatt tgattaatga tgccattgat caattgaaaa acggtggcga aatcagtgat
     8281 ttactcaatg atattgtaga tcatcaaact gatatgatta ctggtcaaaa tggtggttat
     8341 gttttattag atcctaaaga agcgcctagt cgtattttga ttatggacac accagataag
     8401 aataccgcaa ggaatgtttt acaaatcaac aatgctggta ttggtttttc taaaactggc
     8461 attaatggaa catatgaaac ggcatggacg ttagatggcg gattcaatgc ctcgtttatt
     8521 acggctggtg aaatagtagg aattactatt agaggtacta cattaattag tgatggtgct
     8581 gattatagaa caagtattgc taatggcaaa atgacttggt actcaaaaaa agttaacaaa
     8641 gatattatgg agctagaagc acgtgattat gtaagtgctg atgccggtat tgtatcatac
     8701 accatgaaaa ctggtggtgg tttcatgatt agaaatccac agggtaactt ggtttttagt
     8761 acgtgggata atggtaataa cagaccgttt ctatcttttg gtgcgcccaa tttcaggtat
     8821 agcaatgcta gttatgtaac ttctggcgac ggtagttctt taagcattaa tggtagtgcg
     8881 ggtaactcat gggaatttaa ggtagctggt aggactatga aatttactag tgatggtatg
     8941 ctaacgttac caggttgttt ttttggttca tgggaagatg ggaaacttgc taggtttgaa
     9001 caatcaacgg tacaagtata taaagatttt actgttagag gtactaaaaa ctcaactgta
     9061 ccaacagaac attatggaca acgactattg aacgcttatg aaactccaga atattatttc
     9121 gctgattat
//
LOCUS       DAYLZO010000110         8809 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.112, whole genome shotgun sequence.
ACCESSION   DAYLZO010000110 DAYLZO010000000
VERSION     DAYLZO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.112"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            2..748
                     /gene="pstB"
                     /locus_tag="ACGVJG_002420"
     CDS             2..748
                     /gene="pstB"
                     /locus_tag="ACGVJG_002420"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFJ6558334.1"
                     /translation="MISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGKSTYL
                     RSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSIYENV
                     IYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIARVLA
                     VNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTAFFLD
                     GHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            761..1438
                     /gene="phoU"
                     /locus_tag="ACGVJG_002421"
     CDS             761..1438
                     /gene="phoU"
                     /locus_tag="ACGVJG_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFJ6558335.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            1676..2056
                     /locus_tag="ACGVJG_002422"
     CDS             1676..2056
                     /locus_tag="ACGVJG_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558336.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKKMMA
                     GFSSSQVSPLAPCCLLAENRVYLL"
     gene            2017..2709
                     /locus_tag="ACGVJG_002423"
     CDS             2017..2709
                     /locus_tag="ACGVJG_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFJ6558337.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            2706..3473
                     /locus_tag="ACGVJG_002424"
     CDS             2706..3473
                     /locus_tag="ACGVJG_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFJ6558338.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            3573..5141
                     /gene="liaX"
                     /locus_tag="ACGVJG_002425"
     CDS             3573..5141
                     /gene="liaX"
                     /locus_tag="ACGVJG_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFJ6558339.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            5438..5758
                     /locus_tag="ACGVJG_002426"
     CDS             5438..5758
                     /locus_tag="ACGVJG_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFJ6558340.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            5758..6108
                     /locus_tag="ACGVJG_002427"
     CDS             5758..6108
                     /locus_tag="ACGVJG_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFJ6558341.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            6275..7213
                     /gene="hprK"
                     /locus_tag="ACGVJG_002428"
     CDS             6275..7213
                     /gene="hprK"
                     /locus_tag="ACGVJG_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFJ6558342.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            7228..8058
                     /gene="lgt"
                     /locus_tag="ACGVJG_002429"
     CDS             7228..8058
                     /gene="lgt"
                     /locus_tag="ACGVJG_002429"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFJ6558343.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            8102..>8809
                     /locus_tag="ACGVJG_002430"
     CDS             8102..>8809
                     /locus_tag="ACGVJG_002430"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFJ6558344.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPL"
BASE COUNT         2879 a         1491 c         1883 g         2556 t
ORIGIN      
        1 aattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
       61 caatctaagt ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa
      121 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
      181 cggaagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
      241 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
      301 aaatgtcatc tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt
      361 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
      421 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
      481 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
      541 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
      601 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
      661 cttgatcgaa tacaacgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
      721 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
      781 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
      841 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
      901 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
      961 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
     1021 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
     1081 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
     1141 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
     1201 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
     1261 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
     1321 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
     1381 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
     1441 tgaagctgtg attatgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
     1501 aagctgttct gcatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc
     1561 gtttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg
     1621 tttgtgacaa aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca
     1681 aaaaaaaatt agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca
     1741 gcatcaagct gctttatatg aagtacctgt catcgagatc gaaaaagtac gaataaaaga
     1801 ggatcatcaa ttgattacag gaagactgat gaacacggat caaaaaggag aatcgattta
     1861 tttatcggtt ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat
     1921 gtatctagtc caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa aaatgatggc
     1981 tggcttttct tcttcacaag tatctccatt ggcgccatgt tgcttattgg cggaaaacag
     2041 ggtctatttg ctttaggaag tctcatcatc aatatcgttt tacttttact tttgttgctc
     2101 atctttacta aaacagaagg gatttctcta ttaaatatat ctatcctgtt cgtcgttata
     2161 gggatgatta ttagcatatt ggctttagat ggatggaata gaagcagtat aatcaaaatt
     2221 tcagctgcag ttacgagtgt ttttttagca ttttttattt gtcttttgac aatgaatcat
     2281 tggaaagacc aaggactgcg ctatgaggaa ctgaattatc tgacacgacc gtatcgttct
     2341 gtttttcttt ctagcgtact gattggaaca gctggaggaa cactcgatac cgttatcaca
     2401 gtcattgcga cattagaaga attgactaag cgacaaccag aaataacagc gaaacagtta
     2461 tggctttcag gaaggcaagt gggtcgagat gtgattggct cgatgaccaa tatccttttg
     2521 ttcgtctata tcagtgggtc agttccatcg cttcttttat atttaggaaa tcaatggtcg
     2581 ttcaaagaaa cgatagaaca gcatttatca ttggaatttt tacgcgtcat tgctggcagc
     2641 ttaggaatag tattatcgat ccctatctcg gtcctctttt ttctgactgt aaggaggctg
     2701 aaacgatgaa tgtacttatt atacttgttg ggattttagc aatcagcgtg ctacttattg
     2761 gcggtacgca aggtatgtat attcttttag gcttattcat caatttaggg atctttttcc
     2821 tgttgttatt cggttatcat cagaagtggc cgatactggc attgtcggtc ataggatttt
     2881 tattgattgc tgtggttatc ttatttttta tcaacggtta taatctaaaa atgcgtgcag
     2941 cttttgtgag tatactgatc tttttatttt gtttcctact attgcttcct atcactgatt
     3001 ttttagcgat ccaaggtttt acatcggtag aactagaaga attatctggc ttagataaga
     3061 ctttagcgat tgattttaga ttacttacga gatcgttgct tctgattagt cttagtggtg
     3121 ctgttttaga tgctagtgta gcaatttcta gtgggacatt cgaagtatat caggcaaatc
     3181 cgcaattatc tttttaccaa ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg
     3241 cgtcaacagt aatgacactt ctttttgcat tcatgggaag ttctttagcg ttgatcctct
     3301 ggtttattga tttagacatt ccttttcagc aagtgatcaa tgaaaaatca tttgttctag
     3361 aatatacgat ggctattctt accacattgt ctgcactact tgttcttcct ttaacctgcg
     3421 tgactacagc atatctcttt acaaaaaaga aagaacaact caagatggag taaaagttac
     3481 aacttaagtc ctatgacatt tttactgaat cagtgcataa tatggatgta aggaaaagct
     3541 tgcaaacaat tttgcttaag gaggcaattt ccatgaaaga aagagaacgt attttagatt
     3601 tagtgaaaaa aggaatcctt tcgactgaag aaggcttaga tttattagaa agcatggcaa
     3661 ctgaaaaaga cgaaaagcaa ataaaaaaag aagcagacaa ggtaactgct agtcataaag
     3721 aaaaagatca ggcaagtcaa ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg
     3781 agccagttga tccaaaggaa agagaaaggc aagatcaaga aaacttagaa aagatcttag
     3841 atgaactagc aactgaagcg aataaaactt cggcgcgtct agatgaagta aatgctcaat
     3901 tcgctgacaa taaggcagca cgcaatgaga aacaggaagc attgatgcag ctgaatacaa
     3961 aagaagaatt aggcgagttg acagaagaag aattagcaca gcgtcagacg ttagaagcag
     4021 aaatcaaaga attagaagct agtggcgata cactgttaga agaacaattg aaattagaag
     4081 cagaattaaa agatatccgt aagaatcaat ggtctgagaa aaaagaaacg tttactgata
     4141 agtttgaatt gccagatgat tggaaagatc aagcaacaga tacactgaat caagtaggcg
     4201 aaaaaatgtc agaagctgga tcacagttag gcaaattctt gaagaaaaca ttccaaactg
     4261 tttcagaaac agtgaatgac aacatggaat ggaaagatgt cagcttacgt gtccctggga
     4321 ttgcgacaac gaaatttgag catgaatttt actatgaagc tcctgcagca agtatccttg
     4381 atatcaaggc agcaaacgga aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag
     4441 ttgaagctaa gatcaaactt tacggaaaaa tgggtgctga accatttgaa gcattctctg
     4501 aacggagcca gattgaggtt aatgaagatc atatctcctt ccaaattcca aataaacgtg
     4561 tgcgtgcaga ccttgttttc tacttaccta aacgagtata cgaccacgca gctattaaac
     4621 ttctaaatgg caacatcatg attgaaacat tggaagctaa agatatttat acaaaatcta
     4681 cgaatggtaa catcatcgtg gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa
     4741 acggcaatat cgatattcga aacgggaaca tcttagattc tattatcgaa acagtaaatg
     4801 gtacagtgac atttggtgcg acagcagaaa acctttctgt gtctttggta aatggtgatg
     4861 tacgtcttac gattaaagaa gataatttga aaaaagtaga agcaagttca gtgaacggaa
     4921 atgtcaaagt cgctttacca gatggcatcg gtttggaagg acatgctaaa acaagtcttg
     4981 gaagtatcaa cagccgtctg tccaattacg aagtagttcg tgagaaaaaa gaacggacaa
     5041 accaaatgtt gcaattccgt cgtgtttcag atggtgaaat cacgcaagtg caactttcaa
     5101 cgacaacagg aagtatctat ttaaaagata cagataaata aagaggttgt gaaagagatg
     5161 ttcagccttg agcaataaga agcgattttg taaaatagct tcccctattt tacaaaattg
     5221 tgccttattg ccgataggct atctcttgaa caccgtttac caagagatta tgaacctgcc
     5281 aattatcgcc gagtcacaag gaataatttc agaaaaagct cgtcacgttt tatgaatatg
     5341 gtaatttgtg accgagaatt ttgcagaaag acaccgatta agaagaaggt tgtcacaacg
     5401 attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa agttgacaaa
     5461 atccaataaa aatgttgttt tgacagggac tttagcagga ataggagaat acatcgggat
     5521 tgatccgact gttatccgtg tgctttatgt attcctgagc ttagttgctt ttggaagtcc
     5581 aatcatttta tacattttat tagctttgat tatcccatct gcgcgttctg cacgaacaaa
     5641 ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca
     5701 aacaaaaaaa cgcaaagaag cggaaaaaat cgatgatgac gaatggagtg acttttaatg
     5761 tcgtactttc aacgtatttt agttaatacg ttaacattca tatcattggc tgttcttttt
     5821 cctgcgatgg tccatgtgag cagtatttgg acggccgttc ttgccagctt cgtattgtct
     5881 gtgttgaata tgtttgtcaa accgattttg acgatactgt ccctgccctt tacattagtc
     5941 acattgggat tgtttacgtt tgttatcaat gcagcgattc ttaaaatgac ctcctttttt
     6001 gtgggagcag aacattttag tttttcttca ttcggtgcag cgttgttagt agctatcttg
     6061 atgtcgcttg tcaatgtaat tgtcagtgaa cataacagta gaaaataaaa gaaagctgtg
     6121 ccgaactatc ggcgcagttt ttttatttag aatcaggaat gcgttttcta ttacattggt
     6181 aattaaataa aaacgttttt tttaattggt agtacttttg gctagacaat gataaaatga
     6241 tagagaacac tctaatagaa atgaggtagc atgaatgtca gaagttgtaa aagttagcca
     6301 attagtgaaa aaattatctt tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt
     6361 catcgtaact ggggacatct cacgcccagg actagagttg actggttatt ttaattatta
     6421 ttcccataat cgtttacagc tatttggcag taaagaaatc agttttgcgg aacggatgat
     6481 cccagaagag cgtttgatca tcatgagaag attatgtagt gaagatatgc ccgcatttat
     6541 tatttcaaga ggattaccgg ctccagaaga aatgatccag gcagcaaatg aaaatgggat
     6601 agctgtactg cgttctccca tctcaacttc tcgtttatta ggagaattat ccagttattt
     6661 agatggtcgc cttgcccctc gaacaagtgt ccacggtgtt ttagtggatg tttacggatt
     6721 aggtgtactg attcaaggtg acagtgggat aggaaaaagt gaaacagcgt tggagttgat
     6781 caaacgcggt catcgattga tcgcagacga tcgagtagat gtttatcagc aagatgaatt
     6841 aacggtgatc ggagaaccgc ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg
     6901 gatcattgat gtaatgaatc tttttggtgc gagtgcggta cgtggctcta tgcaagtcca
     6961 gttggctgtt tatctggaag catgggcaaa agataagaaa tacgaccgtc tgggctctga
     7021 agacacgacc gttgaaatcg cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac
     7081 aggacgaaac gttgcaatca tcattgaagt agccgcgatg aatttccgtg cacgtacaat
     7141 gggctttgac gcaacgaaaa catttgaaga aagattaagt cgcttgattg aagaaaactc
     7201 aggcaacgac tagcaaggag tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag
     7261 cttgtaggga ttcctatcta ctggtatgca ttgatcatcg tatcaggtat cattatcgcc
     7321 atgtggctta gcagcagaga ggctgttcgg gtaggattaa aagcagatga tgtgacagat
     7381 tttatgttgt ggggattgcc tatctccatt attggtgctc gcttatatta tgttttattt
     7441 gatcttcccc agtatatagc tgatccaatc caaatattca atatccgttc aggaggctta
     7501 gctatttatg gcggactgat tgcaggcgcg atcacgctgt atttttttac taaatatcat
     7561 tttatttctt tgtggacttt tttagatatc gctgcaccaa gtgtattatt agcacaggca
     7621 atcggtcgtt ggggaaattt catgaaccac gaagcatatg gaccagacac gacgcgaagc
     7681 ttcttagaaa gtctgcattt accgcaattc atcattgata atatgtatat cgaagggatc
     7741 tatcgtcaac caacatttct ttatgaatca ctttggagtt tgattggatt tattttgctt
     7801 ttgtttttac gaaagaaacc acaccttttg aaaaaaggcg aagtcctttt atgttatcta
     7861 atgtggtata gtttcgggcg tttctttatt gaaggattac gtacagacag tctttacttt
     7921 ttaggtgtga tccgtatttc tcaattatta tctgctgtgt tatttgtcgg tacattgatt
     7981 ctatttattt ggcgcagaaa aaaaggaaat ttaccggatt atgaccgctc attaggaaaa
     8041 aatcaacaaa ttatctgatt tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa
     8101 aatgaattat agagtagcgg ttttaggacc aggctcttgg ggtactgctt tggcacaagt
     8161 cctcgccgaa aacggacatg acgttcggat ttggggacat cgtgcttcac aagtagaaga
     8221 aatcaacacc caacacacga atcaacgtta tttaccagat cattatctac ccgattccat
     8281 ccaagcattc accaatatgg aagatgcagt aaaagacgca gatgcggtgc tattcgtcat
     8341 tccaacgaaa gcaatacgtt ctgttgcaaa agaactagtg cctaaattga atacaaagcc
     8401 agtgatcatc catgcaagta aaggattaga acagggaaca cataaaagga tctctgacat
     8461 cttgttagag gaaatcccag cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag
     8521 ccatgcagaa gaagtagcga aacatgacat cactacgatc acagctgcaa gtacagatgc
     8581 aaaagcagca aaatatgtac aagagctgtt catgaataat tattttcgga tctataccaa
     8641 tcctgatgtg atcggtgtag agatgggggc agctttgaaa aatattattg ctattggtgc
     8701 tggcgctatt cacggcttag ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg
     8761 attagctgag atcagccgtt taggtgtagc aatgggtgcc aacccgctg
//
LOCUS       DAYLZO010000111         8591 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.113, whole genome shotgun sequence.
ACCESSION   DAYLZO010000111 DAYLZO010000000
VERSION     DAYLZO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.113"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            4..2376
                     /locus_tag="ACGVJG_002431"
     CDS             4..2376
                     /locus_tag="ACGVJG_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFJ6558345.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3906
                     /locus_tag="ACGVJG_002432"
     CDS             2437..3906
                     /locus_tag="ACGVJG_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFJ6558346.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3917..6121
                     /gene="topB"
                     /locus_tag="ACGVJG_002433"
     CDS             3917..6121
                     /gene="topB"
                     /locus_tag="ACGVJG_002433"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFJ6558347.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            6137..6286
                     /locus_tag="ACGVJG_002434"
     CDS             6137..6286
                     /locus_tag="ACGVJG_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558348.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6362..6955
                     /locus_tag="ACGVJG_002435"
     CDS             6362..6955
                     /locus_tag="ACGVJG_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFJ6558349.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6968..7867
                     /locus_tag="ACGVJG_002436"
     CDS             6968..7867
                     /locus_tag="ACGVJG_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFJ6558350.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7870..8343
                     /gene="ssb"
                     /locus_tag="ACGVJG_002437"
     CDS             7870..8343
                     /gene="ssb"
                     /locus_tag="ACGVJG_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFJ6558351.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
BASE COUNT         3292 a         1437 c         1809 g         2053 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacggggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac ttcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagcg
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gctgaactaa agccgaaaca tgaacggcaa aattttttag ttggagacga ccgtttagat
     2941 catgcggttg cttttttatg gaaagatcca cagacaggag agacagtagg ggcaagttat
     3001 caaggaacga tcgtcgattt taatcgtttc ggtaaacggg gaacgtataa acacattgac
     3061 aagaatccta cgccaaatca cggatttaat ttaaaaatag gcgatccgaa gcatttaaaa
     3121 ttctttgaaa gtagtattga tctgttaagt tacgctgcat tgaatcggga gaaactacaa
     3181 gatgcctggt tagtttcgat ggacgggtta aaacatcatg taatcagtca ttacgttgaa
     3241 gagtcgattt ctgaattaag acgaaaacaa acatttcctc aatcgattga aatatgtgtg
     3301 gacaatgatc gtgcaggaca tattttttat gaaaaagaac agatgaaggg aatcgttgat
     3361 ccttttacca ataagaaaat ccgttgtgag cgaggaattc caaatgattg gcaagtacct
     3421 aaagaataca aagctactta tgaagcagtc gcaaaagaaa tgagtgttga acctgaagca
     3481 attatggcca tacacaaaac tgaaactaat ttacagctga caaatcaatt ggtgtctgct
     3541 catgatgttc aatctacgtt tggaaagatg ttggcgaagg gcgaaccagt agaaacaatt
     3601 gatttgaaag aagcatgtac cacggtcgcg aaggaactga aagtatgtga acgcgcagat
     3661 ggaacataca actttgatcg tttttatagt agaaaagcca acataaaaga cgttaatgca
     3721 ggaatccttc tttcctataa agcagaacaa tactacaaag gctataaaaa gcatgaacat
     3781 gaatttgtac ctgaagtgaa aaaggattgg aatgatcaat tgaagcatga gatccagcaa
     3841 caagaaatca gaaagcaaaa acgtgcgatg ttgtttcaac aaggcagaca acaagaaagg
     3901 gagtgaagtc gtcactatga aaaccgtcat tttagcagaa aaaccgtcac aagcgaaggc
     3961 ttatgccgat agcttttcta aagctacccg aaaagatggg tattttgaga tccaagatcg
     4021 attgtttaca ggagaaactg tgatcacgta tggttttggt catttagtcg agttagattc
     4081 gcccgatatg tacgatgaaa aatggaaaca atgggcatta gaacatttgc ctattttccc
     4141 caatcaatac cactatcatg ttcctaaaga taagaaaaag cagtttagtg tcgtcaaacg
     4201 acagctgcag tcagctgata cgattgtgat cgcgactgat agcgatcgtg aaggggagct
     4261 gattgcctgg tcaatcattc aacaagcagg cgccaatcaa ggaaaaacct ttaaaagact
     4321 ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccaacagt tacgagacgc
     4381 agaagagacc tatccaaaat ttgaagaagt acaagcacgt cagatcgccg attggttaat
     4441 tgggatgaac ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag
     4501 tttttcttta ggtagagtcc aaaccccaac actctatatg atttatcaat tgcaggaaaa
     4561 gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gcccaagtaa tcgctcaaaa
     4621 tggcgcattt gatgcgaagc ttgatccaaa tgaaacccaa gcgacacaag aagcatttga
     4681 agcctattta aaagaaaaag gtgttcaatt aggaaaacag ccaggtacga tccttcaagt
     4741 cgaaacagag aaaaaaagtg cggcaagtcc acgtcttttt tctctatcca gccttcaatc
     4801 taaaatgaat cagctcatga aagctagtgc caaagatact ttggaagcca tgcaagggtt
     4861 gtatgaaggg aaatatctaa gctacccacg aacagatacc ccttatatta cagaagggga
     4921 gtatgcgtat ctgttggatc acttagacga atacaaacac tttttaaagg ccgaagccat
     4981 tcctactcct attcatacac cgaatagtcg atatgtgaat aataagaaag tccaggaaca
     5041 ttacgcgatc attcctacaa agacggtcat gacggcagcg gcttttgaac aattaagtcc
     5101 actgcagcag gctatttatg aacaagtgtt aaaaacaacg gtggccatgt ttgcggaaaa
     5161 gtatacctat gaagaaacaa cgatcttgac gcaagttcag cagcttcagc taaagacagt
     5221 cggaaaagta ccagtagatc taggttggaa aaagctgttt ggcaaagaga gcgacagaaa
     5281 agaaaaggaa gaagaaccgc tacttcctaa agtgacaaaa ggagaagcgg tgacggtaga
     5341 tctacaagtc cttgaaaaag aaacgaagcc accacaacct tataccgaag gtacgttgat
     5401 cacagcgatg aaaacagctg gaaaaaccgt ggatagcgaa gaagcacaat ccatattgaa
     5461 agaagtagaa ggaatcggta cagaagcaac acgagcaaat attattgaaa ctttgaaaca
     5521 aaaggaatat atcaaggtag aaaaaaataa gcttgtggtg acaaataaag ggattttact
     5581 ttgccaagcc gtggaaaaag agccgctgct aacaagtgcc gaaatgaccg ccaaatggga
     5641 aagttactta ttaaagattg gtgagcagaa aggaacacaa gctactttct tagctaatat
     5701 ccaaaaattt gtctctcact tgttggaagt cgtaccggga caaatccagt caacagattt
     5761 tggatcaacg ttacaagagg tgaaagcagc cagtgaaaaa caggaagcaa cgcgtcattt
     5821 aggtgtttgt ccaaaatgtc gggaacaaga agtcttgctt tatcaaaatg tggcggcatg
     5881 tacgtctgaa gcctgtgatt ttaaattatg gacaacgatt gccaaaaaga aactcacagc
     5941 cacacaactg aaagagatca ttcaaaacgg ccgaacaagc caatcagtca gaggactaaa
     6001 aggtcagaaa ggttcgtttg aagcaacgat cgttttaaaa gaagacttta caacaggctt
     6061 tgaattttct gaaaagaaaa aaacgaacta taagaaaaga acacgccgca ctacaaaata
     6121 atgaaggggt tatcctatgc gaaatacaac acacaaacaa caagaaatcg aaaatcaaaa
     6181 agcaaaaatt cgtttagaac aagagaaatt acgtgcaaat aaaagaagac tgcaacaatt
     6241 aaagaaccag cagcgcattc aaaaagaaat cgaattagat cgatagacgt taatcttgtt
     6301 acaaataaac tactatgata tatttgatgt agtgtatttg taacgaaggg aggggaagta
     6361 aatgcaagat aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga
     6421 agccaggaaa tttggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt
     6481 gatcaaagtt gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg
     6541 gcttcagcaa cgattttcca ggggaatctt ctctcatgaa acgactttag atatttacca
     6601 gctttctacg aatatgccga aattcattca cttaactttt ccccatggct ataacgttga
     6661 tcgttcaatt acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga
     6721 actaggaaaa gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga
     6781 gcgtacactt tgtgatattt ggaatccgag atatgaattt gaatatgaaa tgaaattaga
     6841 agcattaaaa gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat
     6901 gacaaaacta catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag
     6961 gaaaaagatg acaccgacac aatttgcagc aaaaacaaga aatttagcaa agagtacagg
     7021 attaaatgca cagttagtgc aacggcattt tatgatggat cgtctaattg aacaaatttc
     7081 tgaatcaaag tacaaaaatg acttcatttt gaaaggcggc tttttattag gatcaaaata
     7141 tggaatcgat agaaggacaa caattgatat cgatacgaca tttagaaata gcaaattaac
     7201 tgaagaaaaa ttgaagagca tttttaatga tttaactaga aaacccacaa aagaaggcat
     7261 tgttttctct attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca
     7321 aattaaattg gtggctaatt tagagaaagt acgtgtaccg tttaaaatgg acgtgacaac
     7381 aggagattct atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa
     7441 aaaagtggag attccagctt atccaacaga acaaattatt gcagaaaagc taagtgctac
     7501 gttcagcttt ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc
     7561 gaaactagaa aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa
     7621 acgtggggat acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcagagg
     7681 aaatgactat ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc
     7741 catttcattt acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat
     7801 tgaaaatcaa gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga
     7861 gagataaaga tgattaataa tgtgacatta gtaggacgct taaccaaaga tcccgattta
     7921 cgttatactg caagtggcac agcagtagcg acttttaccc tagccgtcaa ccgaaatttc
     7981 gcgaatcaaa acggcaatcg agaagccgat tttattaact gcgtgatttg gcgcaaacca
     8041 gcagaaacaa tggcgacatt agctaaaaaa ggcggcttga tcggtgttgt cggacggatt
     8101 caaacacgaa cttatgacaa ccagcaagga caacgtgtgt atgtgactga agttgtagca
     8161 gataactttc aactattaga aagtaaagca gcgacagaga gccgtgcgca cgctgatcaa
     8221 tcaaccacca gtccaagccc cactaccttt gaacaacggg atacaaacaa tggattggac
     8281 gcttcacaaa atccatttgg tggacaatca atcgatatta gcgatgatga tctaccattc
     8341 taagtagcat tttctattgg ttgcatttgc tacggtttat gttataattt agtcaacaaa
     8401 aagaattttc aatctataac caaaaaccac tagctggcgg gctagtggtt ttcttttacg
     8461 tttctattat aagaattaac gggtaggctt actgtctttc ttggcaaatt tacaatcaag
     8521 ccatttgcag atatagtgcg cggctacttc tgccatgatt gcaaaaacaa aatcaaaaag
     8581 aattttcaaa c
//
LOCUS       DAYLZO010000112         8588 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.114, whole genome shotgun sequence.
ACCESSION   DAYLZO010000112 DAYLZO010000000
VERSION     DAYLZO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.114"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..570)
                     /locus_tag="ACGVJG_002438"
     CDS             complement(<1..570)
                     /locus_tag="ACGVJG_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558352.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYL"
     gene            complement(580..1740)
                     /locus_tag="ACGVJG_002439"
     CDS             complement(580..1740)
                     /locus_tag="ACGVJG_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFJ6558353.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(1740..2426)
                     /locus_tag="ACGVJG_002440"
     CDS             complement(1740..2426)
                     /locus_tag="ACGVJG_002440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFJ6558354.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(2389..3567)
                     /locus_tag="ACGVJG_002441"
     CDS             complement(2389..3567)
                     /locus_tag="ACGVJG_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFJ6558355.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(3587..5281)
                     /locus_tag="ACGVJG_002442"
     CDS             complement(3587..5281)
                     /locus_tag="ACGVJG_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFJ6558356.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(5259..5573)
                     /locus_tag="ACGVJG_002443"
     CDS             complement(5259..5573)
                     /locus_tag="ACGVJG_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFJ6558357.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(5676..5957)
                     /locus_tag="ACGVJG_002444"
     CDS             complement(5676..5957)
                     /locus_tag="ACGVJG_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558358.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(5962..6306)
                     /locus_tag="ACGVJG_002445"
     CDS             complement(5962..6306)
                     /locus_tag="ACGVJG_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFJ6558359.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(6566..7213)
                     /locus_tag="ACGVJG_002446"
     CDS             complement(6566..7213)
                     /locus_tag="ACGVJG_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558360.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            7423..7614
                     /locus_tag="ACGVJG_002447"
     CDS             7423..7614
                     /locus_tag="ACGVJG_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFJ6558361.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            complement(7607..7948)
                     /locus_tag="ACGVJG_002448"
     CDS             complement(7607..7948)
                     /locus_tag="ACGVJG_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558362.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(7972..8187)
                     /locus_tag="ACGVJG_002449"
     CDS             complement(7972..8187)
                     /locus_tag="ACGVJG_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558363.1"
                     /translation="MKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLTLMDHKFE
                     LNSTKRKIVELIEEEIALQINEQQLAN"
     gene            complement(8483..>8588)
                     /locus_tag="ACGVJG_002450"
     CDS             complement(8483..>8588)
                     /locus_tag="ACGVJG_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFJ6558364.1"
                     /translation="VTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
BASE COUNT         2539 a         1746 c         1203 g         3100 t
ORIGIN      
        1 aaggtagcga tatagccgat tagaatccag ataaaccttt ttccaataat tcgctttagg
       61 gcggttaata acccactgtg cgcctcctga attagtgttt atagtttcca aagattctac
      121 tgtaacattt acaatgtttg ccatatcttt tagaatattt tctccgtttt cacagcttct
      181 aatataatca agacttagat aactacagca gtcgctacaa taccaaacat catctttaga
      241 aggcaatttg cgcaaattaa ttcttttatt gaaaatgaca tccgaatcga tatagaaata
      301 tcggtcgtcc tcacgcgaat gatcttcttc taaatatttc caccataaat atggtttaat
      361 cgaaggaata tactctttgt cgtcccgcag atcatcgtac acatgaactt caacaccata
      421 ttccttctca aaaaaaatag gaatctgatc atcgtgtctg ctgaaaagca atacgatatc
      481 tttgattcct agtttcttca gattagttaa acaaacttca agctcccatt taaaccgatt
      541 gattgccggc tgacaaagaa tatacttcat tctgatcacc tacgcttgtg ttgtagttgt
      601 tgtggttgtt ggttttgtag ttgtagtagt agttcccaaa gcgctaatat ctaatacaat
      661 gaaactatcg ttacgtttag gttgaccgtt tgcatattgt ttagctagat aaatgcgttc
      721 gtcttcaaca aaatggtatt catctgaagc ttcaattttt agtgtagatc ctacacccat
      781 gaagtaatct gaggctaccc caataactgc ttttccttct ggcacagccg ttgactgcaa
      841 atctgaaact ggtactggca atacttgtac gtattctcca ttagcagtta gtacagtctt
      901 agctgggaat actttagacc agtaatcagt tggattcaca attaggacca catcagaagg
      961 attcacatta cgataaatcg gatcattcac accttcgata ttgaattttg atagtcgcgc
     1021 catcaaaccg cccatagtta cagcatctaa agctgtaata ggttctgctt ttttttcagc
     1081 atattctccg ctagtttgtt tgctcatgtc acgcatcatt ccgactggca tatctttacc
     1141 agtaccatca acaattgctt gttctaatgc aattctcaat gattctacta aaacagtacg
     1201 gacataacga tctaaccata ctggacctaa atcaagcatt gccttacata caggaatata
     1261 acctgatagc ttgaactgct tcatgttaat tacatcaaag ccattatcta aaactttttt
     1321 aacagcttcg caaagtttac cccaccatgc tggattgact ccacgtgaca caatccattc
     1381 tgttacacca gttgtgttaa caaaagtaat tttttgcaat agtggatgag attgttctaa
     1441 atcttcaaat acacgttcaa atacagtagc tggcactaat tcttcgaccc ctgcaaaacc
     1501 ttcgtttttc actacttcgt tatagaattt tgtttcttgt gtagttaata cacgctgacc
     1561 acggttcatt aatactaatt gatcttgatt ttttgctgtt gcttcttcta aaattttatc
     1621 ctgaatttcc ttagataagc ttaccatagc tgcgctaaaa gattcttcgt taccatcttt
     1681 aaaagctttc atcaattggt cgcttgcagc tgttacacct tttaaatttt taactgtcat
     1741 tattttacat ctccttgtcc aaatgtttta tttaatgctg ctgtaaatgc agcaattttt
     1801 tctgctcttt tttctttaac gtcattcaaa atttcttcaa cgctttgttc ttttttagct
     1861 tcagtacctg agctattttc tgcatcgata atttcatcga ccaatccata actcaaagct
     1921 gtttctgcat ccataaacga ttctttttca agaagttctt gcaatgcttc atctgtgcca
     1981 ttgaatcgtg ttttatatga agcctttacc gatttatcaa ttgattccag ttggtcagca
     2041 atcgtacgga agtcatcgac atttccttct ccgtatgtgg aagcgcggtg aatcatcaat
     2101 tgtgcattgt tgtagatttt tatagtatcg ccagccattg cgataattga agcagcacta
     2161 gcggctaagc cgttaatcac aacgttaact tttgctttat ttgacttaag taagttccca
     2221 atagcaatcc cttgaaatac gtctccaccg tttgaattaa ttactacttc aatttcttct
     2281 tgatcaccta gactatccaa aatatttttg attcccttgt cagtattccc ttcaaagaac
     2341 caactagaac caataaatcc ctgaataaaa atttgcggta ctgcgccttc attctttact
     2401 gctagaaatg ttttcattgt cgtcattcgc ctcacctcct ttcgatactt gttgattgtt
     2461 tttagttata aatatttcat ctgccatcgc cttatcagag cgatcatttc caacgcgctc
     2521 tcttccttcg ttgattgtaa atactccatt tctaatgcct acatcaatag cgtcaaccaa
     2581 atctttgaag ctagtaatct tgatcatagt tgtatccaca cgtacaaaat tccctgacaa
     2641 gtattcttct acttcataga gactagcgtt aaacgcatcc tgaataagtt cagcaatcgg
     2701 tatgatttcg aacattaaaa aagcgtccac ttgatccgat aacccactca tgtctccctt
     2761 tagtaggttt ttcggaacgt gaaacgctgc tgctgtcatc tcaaagatgt cgtctattaa
     2821 gttttttata tctcttgaat tgctttggaa gtttccgctg aaatcttcta atgtgtactc
     2881 attttgtaat tgaaataccg cacctgcatt atcagcttcc ataaaagcct taaattgtga
     2941 tgtcatcatt ttattgattt gatcttgtgt tgtattgtct tgcggtcgga ataaattccc
     3001 tttcagtacg tatctacgag cgttagagcg cttgtaaaca ttcatggcac tagaaatgag
     3061 tttcccatac gcttgataat acgcatcgac tagttgccta atttgttgat ctgcgtattt
     3121 tatatagata acatcacttt ctagaaattc tctatcaagg actatgttgt taatttgcac
     3181 ttgagaaaac acatcatctt tcaatgcata ttctgtgaca tcccaactat ccgcaataaa
     3241 tatttcgcta gaattattag acggagaaac gatcaatact tcattgtaga atattaatct
     3301 cctgatcagt ttttttctaa attctgttgc attatttttc ttattaggag ctacattcag
     3361 cctatagtaa agatcattct ttttattttt tccatcttca tatgacttga attccgcttt
     3421 actcatcgca tttgcaatca aatcaataca agtttcaatc gcaaattttc gatacacaaa
     3481 atcaacttgc aatttacaaa agtattcttc taaaggaacc gttgcttttt ttgtgaagta
     3541 tcctaccgcc ttttgaaaaa tccccacttt ctcacctcct ttcaagttag aatactagag
     3601 gagtaaatcc agttcctgta ttttctactg agctatttgt gactgttaca ggagcagaat
     3661 cataaatatc atctaaaaaa ttcaaaccat gaaggaatga aaaaaagcca tccgtttttc
     3721 tagtttcagg ttctattttt tcatagcgta tatttccatt agaaatatgc tcttcatata
     3781 cattcatgca ataccaacgc ataatcgcat cgtcaccaaa aaataaacgt tgattaataa
     3841 aaaggtcatc aaccagatct tttaacatac catgtgtaac agatccgctt cgaacaattt
     3901 ccacagtaaa acctgcttct tctaaagcgg gcttcaatat ttttgcacgg tacatatcca
     3961 tagcgatttt tttaatataa tatttattac tcatttcaag aaaccaacct acaatataat
     4021 cagcttctat attttttcca tgaacgatct gtgattttcc ttgatctata gaaatatcta
     4081 taacctctcg tttgatgttt tgtaatcgaa gggctgattc gtggataaaa gtatgttgtg
     4141 taaaataaac atctttatcg tattttccta gcaacccaac gctggcaaaa tctcgtctat
     4201 cagcaaaatc gactgttcct atcacttcat ccattttttc aggaaattct ttttctttcg
     4261 tatgcagaac atcatcatat gaagcaacag caaatcgtgt atcttccata ggtctgttca
     4321 ttcgtttggt catgaacgta agtcttaaac cagcattacg ttgcatttga gagtattctt
     4381 gaaacatttt ccgttttaaa tctgcattgt aattaatagt tggacaagct ttttcccaca
     4441 tgtcgggatc atcaacttca ttatcgttat ccaagcgaca aataaatgga aacaaactag
     4501 aaaattctgc tccatccttg tcaattccaa gttctccaga aagaatcatt tttgattctt
     4561 ctataatgtc atcaagcgga ccaccacgaa catgaccatt agttgtatca taaaattctc
     4621 tataatctcg aattttacca ccaccagaag tagccacatt tatcattgaa taatcttcat
     4681 tttcgtgaat ttcatcaaag cggtttgcac ctggtcgctt cccatctttt gttctagcat
     4741 ttgccgtgtt ataacgaagt ttgctgtttg tagcgatatt ttgaataact tccttcgtag
     4801 ctttaaatac ttttttatct aaatcaggat gatctttaat tactttaaat acatcatcaa
     4861 aactagtctt tgcttggctt tcattattgg catagatatc aatatcataa tttttaatac
     4921 cgtgtttagc ggttagtaga aagaagttgt tccaagaagc aaaaccagtt ttaccattac
     4981 cacgtcccat taatgaaaga tatctattga acactagtgt tttatctttt ttccatcgaa
     5041 caccataaat aaaacattgt agaaattttt cccacggaat taattcgaat ggaaagtatt
     5101 gtgctggtat attgattgaa tcctctacca tctgcttatc gaagtaaata tcttctctag
     5161 taaagactct ttcttctaga taatttttta gcaataattg ctctttgcat accttgatag
     5221 tgccttcttc tatagctttg aaccaatttt caatatgctt ataactcagg aattgattca
     5281 tttgcttcac ctaccaattc aggagtaatg gcaagtttat ccaacatcaa tcccatttgt
     5341 ttgttgacag aaacaagcaa cgctactgat tcattctttt taccattctc cagtctaatg
     5401 ccgttctcgg atatatcttc ttccagtgat atcgccgttt cccataaact gatataacga
     5461 tcaacattat ctaagaatgg ctcaatattt gttttctgac tttccaattg gcttattaaa
     5521 gagcggcgta atttttctct gtagcgattt tgagacaatt cgtttttaaa cattttagcc
     5581 ctcctttcat gataaagttc gaaaaaatct cttttcctga cagccccctc cgtttcatca
     5641 cccccaaaaa atttgcgatt tattttaagg gggggttatc tcaccatcgg aatgaaagct
     5701 tcagcgaagt caatgtaata attaatctct tcaatactat atccaaaaac attttttatt
     5761 ctttcgacgt tattatcttt attcaacgct tctcttactt gattcacttt gtatttacta
     5821 caacagttat ctgataacag atccctaata cctacatagc gaacgtatat caaacgttta
     5881 attaatccct gagtataaga tgaatactct tcaatctttt ctgtatcata ctctctgcca
     5941 ttgtcattga tgatcatgca cttaccacct ttcacttgca tcgaagttag cgaagctttc
     6001 tatcttcttc tcttgtttat ctaacgctgt aagatatctg ccatgaactt cattatgatg
     6061 ttcaacacat aaacaaataa gattatctaa atctaaagct aagtcaggtc tatccttgac
     6121 ttcctttata tgatgaacgt tctctactct atgatactta cctagtcttc tacactcttg
     6181 gcattcatag tgatctcgtt tcatcgcttt ctctctaagc ctgcgccatt taggagactg
     6241 atagaactta accaaacgat cttctcttat caactgtaat aaccatctat agaattcctc
     6301 ggtcatgtcc catctccttt cgcaatctta tttaatactt agctattctt ttgccataca
     6361 atggaataac ttcattgctt tccttccgtt tatatgtatc gctctttatt ggtcttctat
     6421 actttcgtac tatctcaccg ttaccgtttt gcacagtgat tacttcatgc ttctgttcta
     6481 agtattgtgg tctatacatc gtcgtgcctc ctttacgcaa aataaaaaga ccactcaaag
     6541 agtgatctac gaattttaaa aaatctcata ctgaaatctt atttttaatg tctttatata
     6601 agttcgagcc tcgcaaaaca taaatatctt gataatattc tttgccatta tattctaaga
     6661 ataaaaagga aaatttcaat tttttacatt ttttatatct cttttctaat ttcttgctgt
     6721 tctttatgtt agctaccatt gcagtaacta aactttcatt tacaattggt atttttagac
     6781 ctttttctaa gtatccaata ttctggaatt cttgattaat aaggtctgtt ggattgagat
     6841 aagatatttc gtggtccaca tatttttcaa taagcttttt aactacaggg tatttgtaaa
     6901 tatatttgta ccaatgcttt ctgataaaac gttggaaaat gttatattta cctataatcc
     6961 cgcaaaactt tattgcggtt tcagatttcc cttcatttaa taaagttata gtaaacccat
     7021 atttaccgac gacagtaata tcttttttgg ttattccttc agttattctt gatacaattt
     7081 tagaactatt agaataccag tagtactttg atgtttgttg aattgtaatc actatcaaaa
     7141 taatacctac ccattcgcct aacgtaccat atccatcaaa tgtaaaccca agaaatttaa
     7201 ctacgcccac cattttctat cacctcagaa ataatattat caaataatct tagataacta
     7261 aacaggggac ttgtaaattt cagataatta ctcattgact tttcccattc atcctttatt
     7321 gcgtctaccc tttccgccac agcgacaaat tgatattgtg aaattttata catagcttat
     7381 aattttaatt atcagcgagt ggtccgctga aataaattat aggtggtgaa aaatttgtac
     7441 tttgttataa gagaagcaac taatggccaa tattacttcg ttattaagtc caataacaat
     7501 gaagtagtag caactagcga aacatacttg actaaatatt ccgctgaaca aaccattaat
     7561 tctataaaaa acggaatcac caaagactct caagttatcg atatgactaa ataagacgac
     7621 taacttcatt tgctagggag ttcatttcag ctgctttttc taccaaagca gttgagatac
     7681 tttctagctt ttctgattta acagaattaa atactggcgt cttgatacta atcgatacat
     7741 cttgacgaga atcaggactc gttgctctat ttttttgcac cactgatctg tacttttcta
     7801 ctgaatgata ctcaattact tgttccaaat gttttttgta ctctttacat acagcttctt
     7861 tttggatcaa ttctaaacgt tgttgctcaa taatatcaat caaccgttcc cgatccatac
     7921 cttgatactt tatttgttcg acttccattc atttcccctc caatacataa attaattagc
     7981 taattgttgc tcattgatct ggagagctat ttcttcttct ataagctcaa cgatcttcct
     8041 ttttgtcgaa tttaattcga acttatgatc cattaaagta agctttatgt tcttaatctt
     8101 ttcaccttcg aattctttga taatatcttc gattctttgg tttactaaaa ttgttagttg
     8161 atcaattttt tcggaagcga tcttcatttt atataccctc caatacataa attaatagac
     8221 agcaacggat gatagataat aagaacaatt tagaaggagt tgaaattcac atccttattc
     8281 ttaatatttc cgctgctgcc tatcgaagct taattaaacg atgagggaga tttcctccct
     8341 tacattttat tttgtcgatc ctgtttccta atctttcgac actatcataa tacaacgttg
     8401 aataggtaag tgattggtat aaaaaaggta taaaatggaa accaaattgg taataaaagg
     8461 gtataaaaag tgtaaaaact ggctacttga aagcaaccag ttctaacgat gaagcaaatt
     8521 ggatgataat tctgtttgat tctactttaa ccgattcttc gctagtatta ttcctttgag
     8581 cagttaca
//
LOCUS       DAYLZO010000113         8530 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.115, whole genome shotgun sequence.
ACCESSION   DAYLZO010000113 DAYLZO010000000
VERSION     DAYLZO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.115"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            279..1154
                     /locus_tag="ACGVJG_002451"
     CDS             279..1154
                     /locus_tag="ACGVJG_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFJ6558365.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1299..1451)
                     /locus_tag="ACGVJG_002452"
                     /pseudo
     CDS             complement(1299..1451)
                     /locus_tag="ACGVJG_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1719..3050)
                     /locus_tag="ACGVJG_002453"
     CDS             complement(1719..3050)
                     /locus_tag="ACGVJG_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFJ6558366.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3199..3912)
                     /locus_tag="ACGVJG_002454"
     CDS             complement(3199..3912)
                     /locus_tag="ACGVJG_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6558367.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4087..5511
                     /locus_tag="ACGVJG_002455"
     CDS             4087..5511
                     /locus_tag="ACGVJG_002455"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFJ6558368.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5810..6517
                     /locus_tag="ACGVJG_002456"
     CDS             5810..6517
                     /locus_tag="ACGVJG_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFJ6558369.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6504..6827
                     /locus_tag="ACGVJG_002457"
     CDS             6504..6827
                     /locus_tag="ACGVJG_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFJ6558370.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6916..7953)
                     /locus_tag="ACGVJG_002458"
     CDS             complement(6916..7953)
                     /locus_tag="ACGVJG_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFJ6558371.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8274..>8530)
                     /locus_tag="ACGVJG_002459"
     CDS             complement(8274..>8530)
                     /locus_tag="ACGVJG_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HFJ6558372.1"
                     /translation="KEPREHTEKELIGLLRLQLDLQDHYSEIIPRSITGLHRFFKIYI
                     KGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT         2703 a         1604 c         1427 g         2796 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcaatt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctca ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tccataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aaactaaaga aacggtaaag aaagacgatt
     1141 tcaatgaaaa atagcaagta aacgataaca accatcctaa atccacaaga aatacagcag
     1201 accttgcttt aaaaacacga atcatgcaaa ggatgaagta aagaagactt aacgcgtacc
     1261 caatattagt catttgacta ttaggtacgc gttttgtttt ataagtcatg acaaatttcc
     1321 ttcgttgctg ggtagatttt ccggcattgt tgatagattt tatgatactt ttctacattt
     1381 tgtaagagag gttccactgc atctgtatat tggataaaat tctgacaaca actttcaggt
     1441 gtgtcgaacc actctaatct ttaaagataa atattaaaat aacgattaat aatattaaca
     1501 gtctttattt ttcaccacca aaaacctatc tttgcatata ctaaatagac gtatatacac
     1561 agataggttt gtcttgtgaa aaataatgat tatccttacc aaaaaatgtt tgtttttcta
     1621 catgttcgta cttatcaaaa aatatgatcc ttattgaaat tactggctat ttatattcca
     1681 acgacttctt gtgttaattt ttgttatctc atagtaaatt atgtttcatt ttcacttaat
     1741 ttagttgtca cgtgttcgtc aattcgtaaa aacggtaaat atataaacac acttgcggct
     1801 ataattaata tttgtaaaac agctgctcgc cagtctccgg ctgttgctaa aaatcccgat
     1861 ataactggtg gcgtagtcca aggaatttgc actactacat gattaattaa tcccacagca
     1921 gttgcaaaat atgctactgt caaagaaaaa atcggtgcaa gcacaaaggg aataatcaga
     1981 ctgatattaa atacaatagg caatccaaaa ataataggtt cgcttatatt gaatatagcg
     2041 ggagttaccg ataatttagc aaagtcctta taatcttttc tacgactgaa gataaatatt
     2101 gcaatcaata aagcaatcgt atttcccgat ccacccatta ccgcaaaggt atctttaaag
     2161 gccatattga taatatgtgg aatttgctct ttgtttgcat aagctgccat attttcttgc
     2221 atattctgaa tcaaaaacgg atctaacaat gaacctgata ttactgcttg atggatacca
     2281 aatccaaaaa atagattagc aatagatgta attaacaaaa atcctggtaa accagttgta
     2341 attttactta atggctctgt aactatcgtg gtaattagct tatttatatc catattaaac
     2401 aattgaatga ttataaatga caataaagca aacgaggtaa tcgtaataat gattgggatt
     2461 aatacattaa aagactttaa aacagcggga ggaatatttc cggatagctg aatctgtagt
     2521 ttcttattat tagaaagttt aataaataat tctgtagccg ctaatccaac aaatatacct
     2581 acaaacattc ctgaagctcc tgtataggat ataggtatca cacccgtcac ttcaacagct
     2641 ttatccgcat tttcaggagt aaacatcatc tgcataggtg ttaatacaac aaatgtcgaa
     2701 atagcaacta ataccgctgc aatatgattt gtataatttc tgcttgcaca catatgataa
     2761 gatactgctg ctacaactaa taatgtagtt atgcttaatg taccattggc aattgaagta
     2821 ccaatttgtt gtaacgtggt caatgtacta gtatcgacaa tttcccccat gaatcctgtt
     2881 ggttctaaga tcactgaatt aataagtgtc acaaatcctg ctaacatcat aaatggcata
     2941 aatgtagcaa acgcatctcg caaagttctt aaatggatct gattccccaa cttagtcgca
     3001 actataccaa atttttcaaa tacaatgtcc ttcctgctac ttttgcccat attttttctc
     3061 ctttatgctt attgtaatcg attacttttt ccacccaaat aatacatcac ttatcaaaaa
     3121 aagtaaatac ttttaattaa atatatatat cttttttagt ataaaaaaat aagctctgaa
     3181 aaaataattt tcccgagctt atttttcaac aaaaacagct tcaaaaacaa agtcttgata
     3241 taaatgtctc gataccgaat actcgaaagg tttaccagac gatagagcaa caactttgtc
     3301 tatctcaatc ataaattcac ctggatctat ttttaatatt tttgcgtcaa attcattcgc
     3361 agtatctcct ttgatacgta tgtgcgaagt ctttatttcc agacccagtt catttgtgat
     3421 ataagcatat acagatttct ttaaatgttt tggttccaag cctggtatga ctgctaatgg
     3481 catgaatgtt tgctcaatcg agtatggttg acaatcaata aaacggcttc gtacaatatt
     3541 atatacatag tcttttacac tgatacttaa tttatcagct acttttttcg atggaagttc
     3601 aattgagaaa gtatggattt ctgaatctac gtgttcttca ccaaccacgc taaacaaacc
     3661 ttcgtttgga ccaagaactc gtgagttatc aattggtgtt ctgacgactt cagtcccata
     3721 cccacttcta cttcgcaaaa ctccttcgga aacaagtaag tctacgcctt tctttactgt
     3781 taacttgctt gtatgataaa taactgccaa ttcatctcct gtaggaagtt tctcaccaat
     3841 tttatactct ccatttacaa tttttttctt tatatctaag taaatttcca tatacttcgc
     3901 aatttttttc aatggtgacc ctccaatcaa aaacatatct tcatctatac tttaacataa
     3961 ataaaccgat aaaatccaca tcaaaattat acacacaata aaaaagatat atatatttaa
     4021 acaaaactat ttactttaag ttgtaagcgg tgttatcatt aagtggaaac aggaggtaaa
     4081 aaaattatga caactatgaa aaagctaact tttccagatg atttttggtg gggttccgct
     4141 tggtcagctg agcaagcaga aggaaaagga gaaacaggaa aagcagagac tatctgggat
     4201 cgctggttta aagaacaacc ttaccggttt tatcaacgaa tcgattcctc tattacaacc
     4261 gatcacatcc atcaatataa agaagatata gaactaatga aaaaaactgg gcataactcc
     4321 tttcgcgttt ctatttcttg ggcacgaatg tttccaaatg gcggtaccgg aaaagtaaat
     4381 cccaaagcca tttctttcta tcgaaaatta ttttccgaaa tgaataataa cggaatcaaa
     4441 gtatttgcca acttatatca ttttgacatg cctgctactc ttcaagataa aggaggatgg
     4501 gaatcacgtg aagtagttga tgcttatgtt cattacgctg aaacttgttt taaagaattt
     4561 ggtgacttag tttgtcattg gtttaccttc aatgaaccac ttgggcctat tttaggtact
     4621 tatcttgaag attttcatta tccaaacctc attgacttta aacgcggagc gcaagcagca
     4681 ttcttcacta tacttgcaca cgcaaaagca atacaagtat ttaaagaact agctcttcct
     4741 agtaaaatag gcgttattct aaatttgagt cctacttttc ctcgtagtca aaatcctgct
     4801 gatgtgaaag cagcagaaat cgctgattta ttttatacgc ggagcttttt agatcctatg
     4861 gttaaaggga atttttctaa aaagcttatc ctgttattac aagaatataa tcagcttcct
     4921 gaagattata cgaaagaaga tcttgtatgt atctacgaaa ataccgctca aattcttgga
     4981 ctaaactatt acgagccacg tcgcgtcaaa tctcgttctt ctatggtgga tacatctagt
     5041 ccatttttac cagagtggtt tttcgaacca catatcatgc ctggacgacg aatgaacgaa
     5101 taccggggat gggaaattta cgaaaaagga atttatgatt tgtgcatgga tatcaagaac
     5161 cattatggaa acatcgaagc atttatctcg gagaacggaa tgggcgttgc ggatgaagaa
     5221 cgtttcatgg atgatacagg gcaaataatt gatgattatc ggattgagta tatcaaagat
     5281 catcttgctt acttatggaa ggcaataaat gaaggatgta acattaaggg gtatcatctt
     5341 tggacattta ttgattgctg gtcatggatc aacgcctata aaaaccgata tgggctggtt
     5401 tcactcgacc ttactactca aaaaagaaca attaaaaaaa gtggcgaatt ttacaagcaa
     5461 ttaagtgata caaatggatt tagatacgat gtagacttgt tagttgaata atattctaaa
     5521 attccacttg aagtgaacac attagaaaac aagaataatg agaaatacca ataacaaata
     5581 ctttttcttc atacttcatt tatacttgtc agtataagag actgtgacaa atgtagtgat
     5641 cctcagaagt taggtctgga aatctaactt gagaagattt ttttaaacta cgtccagtct
     5701 tttttatttg tttaaaattg aagttgacaa gtcttcatta aaaatttaga atattcacaa
     5761 gtttgacgac aattatgaaa atttatgtcg tgctaaagga gttttgttat tggatgaaaa
     5821 gttagatata aaaactgcga tcaaagacac gttacctacc gtttttggtt atatcggtat
     5881 tggacttgca tttggtatcg ttgggaaagc tgccgggttt catccgctgg tcgtcacgtt
     5941 gatgtccctg ctgatctatg ctggttctgc ccaatttatc acggtcagca tgcttgctag
     6001 ccatagtcca ctgctctcca tcgttctctc gacctttcta gtcaattccc gaatgattct
     6061 gatgagtatg acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa atctgctgat
     6121 tggcacattg ctgacagatg aaagtttcgc actgggaatg aataaattga actatactgg
     6181 acaaaagtta aactttcgtt ggatgaatac agctaattgg atttcttatt tgacgtgggt
     6241 gggttcttct cttgttggcg cacttttagg gaattttatt actgatccga aaaagtttgg
     6301 tttggatttc gccatcgtcg ctatgtttat cgggttactt tatcttcaag tcatttctga
     6361 tagaaacacc tctaaacgat tacagttgat cctgattggt ctcactttga ttctggtata
     6421 tgtcggactg atttttatcc caagtaactt agtgatttta gttgtaacac tgattgggtg
     6481 cggattgggg gtgtggatca aacatgcctt cttttgagtt tacattgctg actattttag
     6541 gttgtacttt agcaacttgg ctttctagga tcctaccatt tgtcttactg aaaaaattcg
     6601 atttgccgca accgttgctt gagtatttaa gttttgtccc gatcgtcatt atgtctgctt
     6661 tatggttcag cagtctattt acccaaaata tcggacatct gccgcagatc aatctcgaaa
     6721 atgctttggc ttcagttcct acacttttag ctgctattct ttcaaaaagc ttattggtga
     6781 tcgtcttggt aggaattctt tccctttcgt tgattcgctt attataaata aaaaaacaag
     6841 agcttatggc aaattactgt catgcgctct tgtttttatc aaagtcaaat aagaaaaaaa
     6901 tcggcgtatt cttgcttatc cgttgaacgg accactaaca acgacttttc ctaatatatg
     6961 tcctgtttct acgtctttgg ttgcttgttt caagttcttc gcattcatcc catctgaata
     7021 gaccttgctc aatgtactgc ggataatccc ttcatttgct aaagcagcaa tgtgcgctaa
     7081 agcttctcct tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca tatactccca
     7141 gtcgaagctg atagattggt ttttccaaac acctatatct aacggctctt tgatccctac
     7201 gatcgttccc acatgtccaa atggtcggac aaggtgtttg atttgatcaa agtagcgggt
     7261 aatatcgaat aatacagcga catactctac ttgatcgatt cctaattcac gaaaactttt
     7321 acttaagtcg tgatgataat cgattggata gtctacacct gtttttttca gccattcaaa
     7381 attcttcgga cttgcagtcg cgtagacttc taggccagcc caatgcgcta gttgattcaa
     7441 aatcgaaccg acgcccccag ctccgttgat cactaagatt tttttcccgc ggttggcatt
     7501 ttcttctgga gtcagaccaa atttttcaaa taacaattca taagcggtca atgaggtcaa
     7561 cggcaaggct gcggcttctt catctgaaag attttttggt gcaagagcga caatacgttc
     7621 gtcaaccagt tggtattctt gattgcttcc tgctcttgtc gttgtacctg cgtaaaaaac
     7681 gcgatcgccg acagaaaatt tttgcacttc atcacctaca gcaacaactt tcccgactcc
     7741 atcaaaccct aagatacgta aagcatttct tatcccgtta tcttggcgaa gtttcgtatc
     7801 gacaggatta acagatactg cgttgacttt taccagaaga tcccgtgaac tgggtacagg
     7861 tatctgttct ttttcatcaa taaagctttt tggatcatcg atcgacaacc cttcataaaa
     7921 accgactacc ttcattaatt ccttttctga catacgaatt cctccttagc ttcatcaagt
     7981 tggagcatcc atacagtttt catttcaata tatatagtat gctatttgtt ccacttttat
     8041 tcatcataat tattctctta cttaaagtaa actatttgca cttttttggt taaaaaagag
     8101 gttgcgacaa gacttttgtc acaacctctt ttaaacggtg ttcaatcaac tgaccctcgg
     8161 tattcgctca aaaaacgaca aaatatgagg agctattttt gtttggctgt tcttattact
     8221 tgatgcagga cagctttttc acgacctcta attcacggtg ttcaagcaag aaatcattgt
     8281 tcactgaaaa gcgttggatg ttctttttca aaggtgtcta agatctctcg tacttcatca
     8341 gtagatttcg tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa tcctttgata
     8401 tagattttaa agaaacgatg tagtcctgtg atggaacgcg ggatgatttc tgaataatga
     8461 tcttgcagat ctagttgcag acgaagtagt ccgatcagtt ctttttctgt atgttccctc
     8521 ggttcttttt
//
LOCUS       DAYLZO010000114         8492 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.116, whole genome shotgun sequence.
ACCESSION   DAYLZO010000114 DAYLZO010000000
VERSION     DAYLZO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.116"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..149)
                     /locus_tag="ACGVJG_002460"
     CDS             complement(<1..149)
                     /locus_tag="ACGVJG_002460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6558373.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACGVJG_002461"
     CDS             complement(593..1714)
                     /locus_tag="ACGVJG_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFJ6558374.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACGVJG_002462"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACGVJG_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFJ6558375.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACGVJG_002463"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACGVJG_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFJ6558376.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACGVJG_002464"
     CDS             complement(4304..5215)
                     /locus_tag="ACGVJG_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFJ6558377.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACGVJG_002465"
     CDS             complement(5232..6239)
                     /locus_tag="ACGVJG_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFJ6558378.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACGVJG_002466"
     CDS             complement(6236..8365)
                     /locus_tag="ACGVJG_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFJ6558379.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACGVJG_002467"
     CDS             complement(8370..8480)
                     /locus_tag="ACGVJG_002467"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558380.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tt
//
LOCUS       DAYLZO010000115         8299 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.117, whole genome shotgun sequence.
ACCESSION   DAYLZO010000115 DAYLZO010000000
VERSION     DAYLZO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.117"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(67..1158)
                     /locus_tag="ACGVJG_002468"
     CDS             complement(67..1158)
                     /locus_tag="ACGVJG_002468"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFJ6558381.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(1175..2341)
                     /gene="aroC"
                     /locus_tag="ACGVJG_002469"
     CDS             complement(1175..2341)
                     /gene="aroC"
                     /locus_tag="ACGVJG_002469"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFJ6558382.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(2343..3407)
                     /gene="aroB"
                     /locus_tag="ACGVJG_002470"
     CDS             complement(2343..3407)
                     /gene="aroB"
                     /locus_tag="ACGVJG_002470"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFJ6558383.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(3440..4462)
                     /gene="aroF"
                     /locus_tag="ACGVJG_002471"
     CDS             complement(3440..4462)
                     /gene="aroF"
                     /locus_tag="ACGVJG_002471"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFJ6558384.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(5121..6095)
                     /locus_tag="ACGVJG_002472"
     CDS             complement(5121..6095)
                     /locus_tag="ACGVJG_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFJ6558385.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(6076..7959)
                     /locus_tag="ACGVJG_002473"
     CDS             complement(6076..7959)
                     /locus_tag="ACGVJG_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFJ6558386.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(7977..>8299)
                     /locus_tag="ACGVJG_002474"
     CDS             complement(7977..>8299)
                     /locus_tag="ACGVJG_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HFJ6558387.1"
                     /translation="ISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLG
                     RILASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLH
                     FKKL"
BASE COUNT         2181 a         1885 c         1487 g         2746 t
ORIGIN      
        1 tgctggtatc ctaacagtcc ctcttaatcc atgtatttgt tgtaataatt gcatttatcc
       61 agcctcctat ttgatccagc aatgataact ggtattagac ataatgattc tttttgcttg
      121 taacaaatcc tgttggtttt taaaactgat ttgcagtatt cctaaaatat cttccctggt
      181 ctcttggatc ttcaaattaa tcaaagaaac tcccgctttt ccgatcagtc cagttacttc
      241 tgcaataacg ccaggttcat ctggcacatc gataaataaa tcaaaaaagg caggaatcgc
      301 gccttgctta tggacaggca actggtctcg actttctttt gcccgattga aaaaagaaaa
      361 gatttccgat tggttctcag tcatgatcca ttctgctatt tgtgtcattt cttgttgcca
      421 atctgccaat cgttccaata atatctgctt attgctcaac aaaatatctg tccacatgac
      481 aggatctgag gaagcgatcc tagtgatatc tcgaaagcct cctgctgcca gttgtttggc
      541 tcgcggatac tcttgattga attgttctgc ctgattgact aagccagctg ccagaatatg
      601 tggaaaatga cttagcattc ctgttatacg gtcatgttct cttgctgtaa gagttacata
      661 ttttgctttg gttccgcaaa atagttcttg cagtttttca acatcttttt tcgtcttttc
      721 caaaggtgta aagatgtaat aggcattttc aaatagttta gcatctgctg cctgtatacc
      781 tgatttatga gaaccagcca ttggatgtcc accgacaaac cgaagatttt tctgctcagc
      841 ggatgtcacg acttgctgct tcgtactgct gacatccgta atgatgacgt gatcagacaa
      901 gttgattctt tcaaggacat caagataagc taaggttgta cggacgggaa cggctaataa
      961 gataacgtct gcttgttctg cacctgtttc aaaatcactt gcaacgtaat caacgatctg
     1021 ctgttctact gcttgctcac gggtttttga ctgactatcc caaccaacta ctatcagttc
     1081 tggattggtc tgacgaatac aaagtgccat agaagcacca atcagtccaa gccccacgat
     1141 aaaaacatgt tgtgccatct tatttgcccc ctttttagta atgtttcaag tagtcacggt
     1201 actccttcac tgcagctttc aactcaacaa gcgaatcact tgggaatttt gacaatagct
     1261 ctgtcgctac cacagtggct acaacatttt ccgcaacgac gctagctgca ggaactgctg
     1321 tactatccga acgttctaca cttgctttgt aaggttcttt tgtatcgata tcaacacttt
     1381 gaagtggctt gtacaatgta ggaatgggtt tcatgacccc gcgaatgaca agtggttcgc
     1441 cattggtcat tccgccttcg aagccgccta gattattggt acgtcggaaa tagcctcttt
     1501 cttcttccca taggatctca tccatcactt ctgagcctgg taatgaacca gaagtgaatc
     1561 cgaccccaaa ttctacgcct ttaaaggcat tgatactaac cacagcttgt gcgattttgg
     1621 catctagctt cgtgtcccat tgtacgtagc ttcctaatcc agctggaacg cctccaacga
     1681 gcacttctac aattcctccg atcgtatctc catttttctt tgtctgatcg atcaaatctt
     1741 ttattctttg ctcaactgct ggatcaacaa ctcgtacttc tgacgcttca gaaatttctt
     1801 tgatttcttg aatcgagata tttccaggta tttctgcttg gattccacca agacaaatca
     1861 cgtgtcctgc tacgatgatg tcaagttctt ccagaatttt cttggcaatt gcgccgattg
     1921 ctacacgcat ggtcgtctct cttgcagagg accgttcaag cacattccga agatctcgat
     1981 gatggtattt cataccgcct accaaatccg cgtgtcctgg acgcggacgt gcaactcgcc
     2041 gcagtgattg gtctttttca ggcacttctg ctacagacat gacagttgtc caatttttcc
     2101 aatctttatt ttctaccact aaagtgacag gtgaaccaat tgttttgccg tgacggatgc
     2161 cagacgtgat ccgaacacga tctttttcga tcttcatcct tcctcctctg ccgtaaccag
     2221 cttgtctgcg agcgagttcg tgattgattt cttcttctga cagaggtaat cctgctggta
     2281 gtccttcaat aatggctgtt aattctggtc catgggattc tcccgcagtc atataacgca
     2341 tcttacagtc ctcctaagta atctttgaat gtctctgtgg gaacaggaac gatttttgct
     2401 tgcccaatct tttctagcaa gatgatattc aattggcttc ctcttgcttt cttatcatgg
     2461 gttatcgcgt ggaataattc atctggatga cttagatcgt acgtaatcgg cagatggaat
     2521 ttttgaagta tcctttctaa agcagctgtc gatccagttg gagacaagcc gattttttct
     2581 gcatgagaag tgatggctac cattccaata gctactcctt ctccatgact gactacccca
     2641 tatcctgctg tattttcaat tgcatggcca attgtatgac caaaatttaa aataagtcga
     2701 tttccttggt caaagacatc ttcttccact acttttcgtt tgatttccaa cgtagcagta
     2761 atgattggaa cagcatgttc cagaagatct ttttcgtctt tcagctcgtt cagctgtttc
     2821 caaagcgttg catctgctat agctgctgat ttgatgattt cagcgattcc ttctcgtaaa
     2881 cgacgtaccg gcaaagtttt tagaacctca ggatcaatca atacaccagc tggctggcta
     2941 aatgttccga caaggttttt cgctttgttt gtattgactg ctgtcttccc gccaatacta
     3001 gaatctactt gtgcaagtag cgtagtcgga atttgaacga aatggatccc acgcatgaac
     3061 gtcgaggcca cgaatgctgc tagatcaccc accattcctc caccaagtgc aagtatccca
     3121 tcgctgcggg taaaatcatt atctgctaaa aaatcgtata actcagctgc ttgttctaaa
     3181 gatttactgg tctcacccgc cggtacgatt gaaaggactg gttcaaaatt agcttgtctc
     3241 aattggaaca tgattttttc tgcatatagt ggagcaacgt ttgtatcagt gatcacagct
     3301 actttttgag gtctccaaat cgtttgaagc catgatccag cctgttctag tgcgcctttt
     3361 tcaatatgaa tatcgtattt ttggttatca acatgtacag taagcattcg tttcacttct
     3421 ttcttttaaa aagctggaat taatcgagtg tctgaatctt ttccattgct tctttcatga
     3481 tcgcaacttc tttcatcatg cgtgagtaag tcttttcatt caacgattgg ggaccgtctg
     3541 accaagcatt tgcaggatca ggatggatct ctacgatcat gccatctgct ccgcttgcca
     3601 cacccgcacg agccattggt ggtacaagtt cccaaattcc tgtaccatga cttggatcaa
     3661 caatcacagg gaaatgagtt aattttttca tcaatgggac agcacttaaa tcaaacgtat
     3721 tacgtgtcgc tgtttcgtaa gtccgaatcc cacgttcgat aaaaatgaca ggagatttgt
     3781 cttcgactgc aatgtattct gcagcattaa gccattcatt gatcgtaccg gaaattccac
     3841 gtttcaatcc aacaggttta cctgttttac cgacagcaga tagcagttta aaattttgca
     3901 tattacgtgc gccaatctgt aggatatcgc tgtactcagc caccatatcg atatgccctt
     3961 catccatcac ttcagtaatc actttcattc caaattcatc agccgcttga cggatgtatt
     4021 tcaatccttc ttcttctaaa ccttggaaag catatggaga agtacgtggt ttgaaggcac
     4081 cacctcgcag aatttttgcg cccccggctt tggccattcg cgcacattct ctgatttggt
     4141 ctaatccttc gatagaacaa ggaccagcca tcgtcacgaa actgccgtca ccaattttta
     4201 ctccgtctac atccacgact gtattgccag gatggaattc tcgagaagtc aatttgtatg
     4261 tgtttaaaat acgcacagca ttttctactc cttcatagcg tgtaaatgct ccttcttgta
     4321 aacttcgcgt atcacctttt agtccaatca ctactcgttc tgttccttga tcgattgaga
     4381 ctttgaatcc ttctttttcg atatgatcga cgactttttt tatttgttct tccgttgctt
     4441 cttttttcat aattactatc attgtttttc ccgctttctt ttgtttactt atctatcttg
     4501 gctgattttc tctacttttt caaggtggtt ttacattaat tcctactaaa tgaccgtgtc
     4561 ctgttgttct cattttcgct ccttctttcc tacttatgac ccagataatc aacaaaaaaa
     4621 gatgtccctg taggaaattg cctctacagt gaacatcttc aacgagcctt aaaaagcgct
     4681 gcaaaaatat aaaatagcca ctatagatga atttctctac gtggctgtaa gtcgtgttta
     4741 aaaatcttta gccatcatag atacaaacct ggtttgtttg tacccacgaa gtcatgaata
     4801 caaacctatc taaaatagct aaagtaatac gtattcagtt ttgtaagttt tctagatttc
     4861 ataaaaaatc gctccaaact gatgtaagaa ttgtacctag tatagacata aaaaaatcga
     4921 ctgtcaatca tttttatgaa acttttttta attttctgat agttaacttt gtttttcaga
     4981 aaaataacaa ttggttttat tgaagaaaat agtcatgaac aaaagtatct gagaactttt
     5041 ttactctctt gttcatgaca tatagaataa agcagctttg tcttgtaaac tccgtttttc
     5101 aaataataat ctaaagattg ctactgaata ataaagccga tttcatggtc ctcttcgctg
     5161 ctgattcggt aaggtgactc aacatccgct ccccagctat tgataccccc gactcctctt
     5221 acttttgcaa aaatcgtaag aaccgttcgt ctttctggcg gtagttcttc tatatgtgtt
     5281 gcctgttcca gttcttctgc tttgaatggt aaagcggaaa aagcaaatgg cgtatcctgc
     5341 tgttcaaatc gcaacgaaaa atcttcttga tctttatccg tattatccaa cgtttgattt
     5401 cttgttagtt ctacccattt cgtttccata tgcatgccgc attcttgggg aacaaggtat
     5461 ggggaaacgg ggagtcctgc cactttataa ataccttcga caccaccagc catccgatcc
     5521 ggatatgttt cccctgatag gccttcatac tcaaaaaatt ttgctttcgt tggcataatg
     5581 aaccttaaac ccaatgcggg taattccggt aatccttttt gtccataata atgcatattg
     5641 acaagcattt taccctctcc agcgatttgg taggaaattg tcacttgggt ttttggtact
     5701 gtgttgattt ccagtgtaaa aatgatttca accgtttcag cgtattcatt acttgaatat
     5761 tgattattta ctggagcaat cggcaaatcg atctttttat tgtccaccag tacttctatt
     5821 tctttgactt ttggatacaa tccggctcct aaccacatac cggatttaaa tccaaatccg
     5881 ctacctctgt cattatcggt cgttgctcgc caaaaaactg gaagtgtttc ccggtatagc
     5941 cattcttttc cgtttttatt gagcgattcc agtccgcctc ttgtataaga aaaaatataa
     6001 tgcgtattcc ccgatttgac acctaatgta cagtcgccga agacgattgc aactttttcc
     6061 gtatccatat gacttttatt tactgtattt ttcataataa tatcttacct cctgcatggc
     6121 gggtttttct tgtccatctg caaaaacgat cccattccct gagaattcat agtcacttgg
     6181 tttatcgtca aagtcaccgc catagcgcat cacttctctg cctgtgattg gatcagtcac
     6241 ttggatcgcc tgatctttaa aatcccatat atagcctcct tggtacattt cataacgatc
     6301 aagcaacgtc atgtaagaat ccattccacc taacgaattc cccatgtcat gcatatattc
     6361 acataagatg aacggttttt ccgctttatt ttcaaggtag tcaatgatct cttctggtgg
     6421 cgcatacatg cagcttttta catctgaaat cgaatcttcg aatgctggat ttggaaaatt
     6481 cccttcataa tggacaagtc gatcggggtc tacttttttg aagtaatcgt tcatttgtcg
     6541 aatgttttct tccgcataag attcatttcc aagagaccaa aaaagaatac ttggatggtt
     6601 ttttaatgtt tcgaaattcg tttttgctcg atcaagaaca gccgcctgcc actgaggtac
     6661 tgcacctggt acgttgtaag aaggctcaaa tgcgcccatt ttttgccatg agccatggga
     6721 ttcgaggttc gtttccgcca tcacatggat tcctgcttgg tcgcataaat aataccaagt
     6781 gatctggttg gggtaatgag atgtccgtac gccgttgatc tttgcatttt ttatgatagc
     6841 caaatctttt tccatatctt ctatagtgat ggcgcgtcct tttttcggat gccactcatg
     6901 tcgatttacc ccgttgataa tcatccgttt tccattgaag tagatgattt tatctttgat
     6961 ttcgatttta cgaaaaccga aggaatagtc aatatactcc gttactcttt tttcttcgtc
     7021 tatcgtctcc aattcaagct tatataaatt gggctgaaaa tgcccccaca gtgccacatt
     7081 ttcaaatcca tcgatgcgaa tcgtcatttg ctcttttaac ggatattgct gttgatagat
     7141 ttccttccca tcaggatcta gtatcgctac attcagctga ctgtttttgg ctacttgctc
     7201 aaaagacaaa tctacttcaa gtttgccatt ttgacatttg tcatctaagc gaggtttgat
     7261 ccaaagatct tctaaatggt tttgtcctcg agcaactaag aagacatctc tgaagatccc
     7321 aaaaaatcgg aaaaagtcct gatcttccaa gaatgcagct gtacttcttt tgtaaacctc
     7381 cacagctaga atattttctt cttccaaata aggtgttaaa tcaaattcag acggagtgaa
     7441 cgaatcttcg gcatacccga taaaatgacc attcaaccaa ataaacattg cttgttctac
     7501 accagcaaaa tgaatcgaga tttgttttcc ctttagacct tcttctaatc gaaaagtcgt
     7561 caaatacgaa ccaaccggat tgtctttagc agctgaaaag atcccttccg cctctttctc
     7621 agggcttgtc gtgaaagggg gacggcgaaa tacatgtccc tcccacggat acatcgtatt
     7681 gatgtaatgg atcttgtcat aatttgctaa ttcaatatga ctaggaacag tgatttcatc
     7741 aaaagaacag gcatcaaaat cttttttata aaatgttttt ggtcgtaccg aaggatttgg
     7801 cgaataacaa aaacgccatg atccatttaa agactgttta agggaggatg tggtttctac
     7861 ttcttccctt gttgcataac aaacatgatc gctatgtgca tttgattggt tcactctaaa
     7921 aacttgtgga tcatctaacc attttatatt tgctttcata cttttttcct tctctctcat
     7981 aactttttga aatgaagcag ttctgtttgg aaatcacctt gaaaatcggg gaagtaaagt
     8041 ccaactgtca tcaattcggc ccccgaaaat atccgcttcg aggtctgttc ctgataaagt
     8101 gcctcatcat ctaaccccat cagatatact tcatggaacg ctggttgagc tgaagctagt
     8161 atcctcccta aaaacacaat agcttcatct tgtttaggag acacaaacat ccatgcggca
     8221 atattttctt caaaaggact cgccaaacga taataatggc catactggat caccggacga
     8281 atcgtttgat aggatataa
//
LOCUS       DAYLZO010000116         8238 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.118, whole genome shotgun sequence.
ACCESSION   DAYLZO010000116 DAYLZO010000000
VERSION     DAYLZO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.118"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..684
                     /locus_tag="ACGVJG_002475"
     CDS             <1..684
                     /locus_tag="ACGVJG_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFJ6558388.1"
                     /translation="ERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFIC
                     AKYLAEKNRLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFG
                     GEQSGHMIFLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDK
                     NGAMDVPAIKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAA
                     VVKEEIGLD"
     gene            complement(756..2561)
                     /gene="glmS"
                     /locus_tag="ACGVJG_002476"
     CDS             complement(756..2561)
                     /gene="glmS"
                     /locus_tag="ACGVJG_002476"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFJ6558389.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2971..3261
                     /locus_tag="ACGVJG_002477"
     CDS             2971..3261
                     /locus_tag="ACGVJG_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558390.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            3274..4299
                     /gene="sppA"
                     /locus_tag="ACGVJG_002478"
     CDS             3274..4299
                     /gene="sppA"
                     /locus_tag="ACGVJG_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFJ6558391.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            4302..4961
                     /locus_tag="ACGVJG_002479"
     CDS             4302..4961
                     /locus_tag="ACGVJG_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFJ6558392.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            5118..6050
                     /locus_tag="ACGVJG_002480"
     CDS             5118..6050
                     /locus_tag="ACGVJG_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558393.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            6204..7493
                     /gene="tig"
                     /locus_tag="ACGVJG_002481"
     CDS             6204..7493
                     /gene="tig"
                     /locus_tag="ACGVJG_002481"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFJ6558394.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            7740..>8238
                     /locus_tag="ACGVJG_002482"
     CDS             7740..>8238
                     /locus_tag="ACGVJG_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HFJ6558395.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQ"
BASE COUNT         2736 a         1408 c         1770 g         2324 t
ORIGIN      
        1 gaacgtttag ctgagatggt cgttgaaaaa ggagcagatg ctggactagc tttcgatggc
       61 gatggcgatc gtattattgc ggtggatgaa cttggaaaca ttgtcgacgg tgataaaatc
      121 atgtttatct gtgctaaata cttggcagaa aaaaatcgat tgaaaaaaga tacgatcgtt
      181 acgactgtca tgagtaactt aggattccac aaagcggtgg aaggaatcgg tttgaaagat
      241 gtcgttacac aagttggtga tcgttacgtt gtagaagaaa tgcgcaaaaa cgattataac
      301 tttggcggcg agcagtctgg gcacatgatt ttcttagatt ttaacacaac tggagatggg
      361 atgctttcag gaattcagtt actgaatatc atgaaacaga caggtaagaa attatcagag
      421 cttgcagcag aggtaacaat ctatccgcaa aaattagtga atatccgagt aagtgataaa
      481 aacggtgcga tggatgttcc ggcaatcaaa aaagtgattg aagaggcaga agctgaaatg
      541 ggatcagaag gaagaatcct agttcgtcct tccggtacag aaccattact tcgagtgatg
      601 gcagaagcgc ctactgatga gaaagttaat tactatgtcg ataaaatcgc ggcagtagtc
      661 aaagaagaaa tcggactgga ttaagagata ttttcataaa aaataaaaga acaaggacca
      721 gtgtcagatg tctgacactg gtccttgttc ttttattatt ctactgtcac tgatttcgct
      781 aaattacgtg gtttgtcaac gtcgaatccc cgtaatagcg ttgcgtaata tgcaagtaat
      841 tgagtcggta cgactgtggt tagagtagtt agataagggt ggacagttgg tagcaccagc
      901 tggtcacttt ccttttctaa tccctctgag acaataacta gtgttttagc gccacggctt
      961 tcgacttctt tcaggtttcc tcgagtatgc gcaccagtca cttcttcact gatgattcca
     1021 atcactggtg ttccttcttc gatcaaagca atcgttccat gtttcaattc gccagcggca
     1081 aaaccttctg cctggatata actgatttct ttcaatttca aagcagcttc aagagaaacg
     1141 ttgtagtcag cgccacgtcc aatgtaaaag gcatttcttg tatcgctgag atatacttct
     1201 gccaactctt ctaaataatc tttttgggcg atgaccgatt ccatcgcatt agcaactaaa
     1261 ctcaattcat gagtgatatc gaatacaaga gaagtttcaa attctttttt atcaccgata
     1321 gctttggcta ataaagttag tacagctatt tgtgcagtat aagctttcgt tgatgctact
     1381 gcgatttcgg gtccagcatg caatagcagt gtaaaggatg cttcccgcga aagagtagaa
     1441 ccagcaacat ttgtaatcgt aagtgaaggg tagccgagac ggttgatttt taccagaact
     1501 tggcggctat ctgctgtttc gccactttga ctcaagaaaa taaagaatgg tttttttgaa
     1561 agaagaggtg tgttatagcc aaattcactg gctaaatgaa cctcaacagg gatctgtgtc
     1621 aatttttcca gagtctgttt acctgctaac cccgcatggt aactagttcc gcaagcaacg
     1681 atatagattc gatcgctatc agacaatgta tcgaccagct tttgatcaag agagacacga
     1741 ttgtcttctg ttacgtattt ttgagcgata cgacgcatga taataggctg ttcgtcgatt
     1801 tctttcagca tataaaaagg ataggtacct ttctcgatat ctgaagaatc tacttgtgct
     1861 tcgaatgaag aacgttctac gatgtcacct gcaaaatttt gaataataac tttttctgat
     1921 gtgacggtaa ccatctcttc atcttcaatc tcaacgaatt ccttcgtatg agcaagcata
     1981 gccattgcat cgctggcaat cacattgaat ccatcgccga gtccgattaa tagtggactt
     2041 ttatttttag cgacgtaaat aacatcaggt gctgtccgat caatcaaagc gaaagcgtaa
     2101 gaacctttga tgacccgcaa agcttttaaa aacgcctctt gcgttgtagt atccttcgcg
     2161 aaagtttcaa ttagatgagc gataatctca gtatcggtct caccaataaa gtggtcattt
     2221 gctaaataag caattttaag ttcttcaaag ttttcgatta cgccattgtg aacgagcacg
     2281 aagcgtccac tttgggaagt atgtggatgg gcgttttctt cagaaggttg cccgtgagtt
     2341 gcccagcgtg tatgaccaat acctatattt cctgtagtat caatatttat tttgtttttc
     2401 aaattttgaa ttctgccttg tgctttgacc agataatcag ttgtgccatc tgagacaaag
     2461 ataccagctg aatcataacc acggtattct aatttttcta gaccgtcaat caatcctggt
     2521 gttacatttt ttaatccaat cattccaaca attccgcaca taaaaaacat cctactttct
     2581 ataatgaatt cgtatgaatg cttccagctg caggaaaatc cttttgtttt atctttgcag
     2641 atattttttg tggattttct gtagagtgca gtccctaggg ccatccgccg aatctttcga
     2701 taaaccctct cctcgtcatc tgtttacaca gacctggcgc tatcctaaaa ggtataacta
     2761 tcctcctctt ttgtttttta tctggaaaca tttaaaatca taaccttttg cgaatgttct
     2821 tgtcaacaaa aaaagaataa aaaataaatt ggtatatatc aaatgctttg tcaaatgctc
     2881 tttttatgtc aaaaaataat gggtttttat ctatcatctt tttcaggagt tcggtacaat
     2941 agaaagtaat cggatagagg aggagtaaaa atggattcaa aaattgagat catgacactg
     3001 ggaatgttaa aaaaacaatt gtccaaattc gaagcatcag ctggtgtatc agatgatacg
     3061 aaaatatttt tagatactgg ctgggatagc atccaagaaa tcgcacctga tgccttggaa
     3121 gttgctgaag ctcgtgagtt tacggtagaa gatgaattga caaaagagtc gttttccggg
     3181 tatgcgcggg aagaaaaagc agagcggttc gacacttctg agcagtcaga aactgtgatc
     3241 gtcataaaaa acttgtatta gaaaggagaa agaatgaata agagacgttg ggcagcagtt
     3301 ctgattgctg caggattatt tgtggtttcg ttagtttcag ctagtttgac taagcctcag
     3361 gaaaacgatc aaattgaggg attgaacagt tggttatatg gggatgaaga actagcttca
     3421 aatgtgttgg aagagggaga cagtacgcaa aaaatcgtaa aattatcagt agatggaacg
     3481 attgctgcgg gagggtcttc aggtttattt gcttctgaag gttacaatca taaaaatttt
     3541 atggaacaac tgaaggcgat tgaagaagat ccaacagtca aaggtatctt tttagaagta
     3601 aattctcctg gtggcggtgt ctacgaaagt gcggaaatcg ctaagaaatt ggatacgatt
     3661 cgtaaagaac atgatatccc aatgtatgtc agcatgaaaa acatggcagc tagcggtggc
     3721 tactatattt ctgctcaagc agataaaatc tttgcaacag aagaaacagt gaccggctca
     3781 attggtgtga tcatgtcagg attgaattat tcaggacttc tagaaaaact gggtgtggaa
     3841 gatacaacag ttaaaagcgg agcgttgaaa gatatgggtt caggaacgcg accagagaca
     3901 aaagaagaag aagcggtgct tcaagcatat atcgacagcg catatagtcg ttttgtagca
     3961 atcgtcagcg agggacggaa taaatctgaa gaagaagtca aaaaaattgc tgatggaagg
     4021 atttatgatg gtgtccaagc aaaagaagta ggtttagtcg atgaattggg atttcctgaa
     4081 gatgctttaa aagctatgcg aaaggaacaa cagcttgaag atgcggaact agtagaatat
     4141 tcttccacgc catctgggtt tgctagtaca tggttaggaa gtaaactagc agaattccag
     4201 ggattgaagc cttctgagac tagtcagatc ttatccgttc ttgagaatct agggacagcg
     4261 gaatcaccaa aagcaatgta ttattacgga ggtgagtaag catgtcagag ctttcaaatg
     4321 aaaataagga acaagaaaaa gataagcaac agcagcctgc tgtaggacct gactccaact
     4381 tttcccaaaa ggtactgagc tcttttgaaa gagagccttt gacgcccgaa gaaatcaaaa
     4441 agaaacaacg taaatggaaa aagtatacgg acacaagaaa tgaaccgcca aaagcaaatg
     4501 attttcctaa gtatttctat gcaggttttt ttatccgaat gtttgctttc ttggtcgatc
     4561 ttttgtgtat cggagctatt acgcgtttaa cacttggtat tgccaaaaat ctaggttggc
     4621 ttaatttttc agattcttat ctgagtatct acggattcac agccttactt atttatctag
     4681 cgtattttat tttattgacg aaattcaatc atggtcaaac gattgggaaa atgatctttg
     4741 gtattcgagt catcagcttt gatgaagaag aacttagctg gtcaaccgtt ttagttagag
     4801 aaggtgcttg ccggttcatt ctaaaatttc ctttgctgat gttagggtat cttccagcaa
     4861 tctttaatca gaaaaagcaa catctaggcg attttttcac taatacaagt gttgttacga
     4921 tcaatctgat caaagcattc aaccaggaag tcaacagctg atgagcaata aaaagcagta
     4981 acagttgaaa gcaccttcta aaagtcagat ctaaaatcta gcttttagag ggtgcttcag
     5041 ttatttttaa ttgtcatgct ttttttgttt tgctactatt gaacatgagc tgaacatgag
     5101 caagaggagg acactgcgtg aagccgattg attttccaga aaaatatgag aatttaatgc
     5161 gagtagcaca acaagcacta gcaaaccagc aatatcagca ggcaaaagaa ttatttcaac
     5221 gagcttacga gttgaaagaa agctttgagg ctaatagcct gcttgttttt tgtctttacg
     5281 agttggacga gaaaaaagaa gcattgaagc aagcactctt gcatgaaaaa cagtatcttc
     5341 aaaatgagga gtttgctgaa ttttattttg acttgttaat cagtacacaa gattttttat
     5401 atgctagaaa attgatagca agtacagatt tttatgaagc tttcgagcag cgggtcattg
     5461 aaaaaatcca atttgcagaa gaattatccg gacaaatgga gaggcagaag atcaaggcac
     5521 tccacaataa atcggaggaa cttccttcgt tggaacctgc acgtcagctt gcgtctattg
     5581 atgagatcga acagcttcct tatcatgagt ttatccaaac agcgagtaaa cttattgtat
     5641 cgccagaggt ccatatttta gcaagggcaa aattactgga gacacttagg caattgaacg
     5701 aatgccatcc tgttttctat ttgacgattg aagaaaagtt agtcaaagtc ataccaaaag
     5761 atctgccaaa gccgcagcag caggcaagtt accagcaatt atgtgtattt tcagatcgtt
     5821 acggtaatga agatgcgctg cttagttccg tgttaaatga agaattcacg ttgcaatctg
     5881 caattgttta tcctgtctat gatacataca tcgaagaccc gaagcgctgg tttcaactga
     5941 ctgtagaggc gtgtacagga aaaacgatgt atgaaggtac agaagatgaa aaacaagatt
     6001 ttttaaaaaa aagagaaaaa attttgcagc aaatgatctt cttccattag attttatcag
     6061 tgaaaaagat aaaaatgtta tgaaaaacaa ggattctatc cattcaatat gtttacaatt
     6121 tgaaagggtt agtgtactat ttaaaggtat gcatgaaacg tttttgatgt ataaaatgaa
     6181 tttctaaact cggagggaat taaatgactg caacatttga aaaaaaaggc acgaatgatg
     6241 gtgttttaaa atttacgatt gcgcaagacg aaatccaaaa aggattaaca actgcgttta
     6301 ataaagtgaa aaaaagcttg aacgtacctg gattccgtaa agggaaagtt tctcgtcaag
     6361 tattcaaccg tatgtacggt gaagaagctt tatatgaaga tgcattgaat gctgtattac
     6421 cagaagctta tgaagctgct gtcaaagaag ctggcatcga tcctgtttct caaccaaaaa
     6481 tcgatgttga aagcatggaa aaaggtcaag actgggtaat cactgctgaa gtaacagtga
     6541 aaccagaagt aaaattagga caatataaag atctagttgt tgaaaaacaa gaccgtgaag
     6601 taacagatgc tgacgtggat gcacgtatca aacgtgaaca agaagcacaa gctgaattag
     6661 ttatcaaaga agatgctgca gctgaaaatg gcgatacagt agtaatcgac ttcgaaggct
     6721 ttgtagatgg cgaagcgttt gatggcggaa aaggcgaaaa ttactcactt gaattaggtt
     6781 caggctcatt cattccaggt tttgaagatc aattagttgg tcataaatca ggtgaaagtc
     6841 tagatgtaaa tgtaactttc ccagaagatt atcaagcaga agatcttgct ggaaaagaag
     6901 cagtcttcaa agtgacaatc catgaagtaa aagcaaaaga agtgcctgaa ttagatgacg
     6961 aattcgcaaa agatgttgat gacgaagtat caacattaga cgaattgaaa gaaaaatatc
     7021 gcaaagaatt agtagaaaca aaagaaaaag cagcggacga tgcaaaagat gaagcagcga
     7081 tccgtatggc tgttgaaaac gcagaaatcg ttgaattgcc acacgtgatg gttcatgatg
     7141 aagtacatcg ttcaatggac gaattcttga acaacatgca acgtcaaggt atctctcctg
     7201 aaatgtacta tcaattaact ggttctacag aagaagactt gcataaacaa ttcgaaggtg
     7261 aagcagaaat gcgcacaaga acaaatcttg tgatcgaagc aattgcagct gctgaaaatc
     7321 ttcaagcaac agaagaagaa atcaatgcag aaatccaaga attatcagaa acatacaaca
     7381 tgccaatcga acaaatcaaa cgcgtgttga cagaagatat gctggaacat gatgtgacaa
     7441 tgaaaaaagc aatcgaattg atcacaggta cagcgacaga agcgaaagaa tagaaatcaa
     7501 gtaataaatc atttctgaag aagctacttc tagaaaattg tttattcgaa ataagggact
     7561 gtgacacgat tttgtcacag ttccttattt tttgttaaga gtaaaaaatg caaaggtata
     7621 ggtcagttga tcctttgtta aacacttttt ctttttacta aactatgtta tgatagtaaa
     7681 agactgtaaa gtctttgata gtttcttgca tactttttgc aagttaaggg gtgaagacca
     7741 tgtatgacaa tccaagtagc aatgagaccg tacgctgttc tttttgcggg aaaacccaag
     7801 aagaagtgaa aaagatcgta gctggtccag gggtctacat ctgtaatgaa tgtatcgacc
     7861 tatgtaaaga aatcattgat gaagaatttt atgaagaagc tgtacgcgaa ttcactgaag
     7921 tccctaaacc actagaaatt cttgaagtat tgaataacta cgtgattgga caagatcgcg
     7981 caaaacgctc gttagctgtt gccgtataca atcactataa acgtgtcaat cagcaagcac
     8041 aagaaaacac agatgatgtt gaactgcaaa aaagcaatat ctgcttgatc ggacctacag
     8101 ggtctggtaa aacattttta gcacaaacat tagcaaaaac attgaatgta ccttttgcaa
     8161 tcgcagatgc gacaagtttg acagaagctg gttatgtggg ggaagatgta gaaaacattt
     8221 tgcttaaatt gcttcaat
//
LOCUS       DAYLZO010000117         8112 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.119, whole genome shotgun sequence.
ACCESSION   DAYLZO010000117 DAYLZO010000000
VERSION     DAYLZO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.119"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(31..297)
                     /locus_tag="ACGVJG_002483"
     CDS             complement(31..297)
                     /locus_tag="ACGVJG_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558396.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(290..613)
                     /locus_tag="ACGVJG_002484"
     CDS             complement(290..613)
                     /locus_tag="ACGVJG_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558397.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(625..1881)
                     /locus_tag="ACGVJG_002485"
     CDS             complement(625..1881)
                     /locus_tag="ACGVJG_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFJ6558398.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            complement(1878..2273)
                     /locus_tag="ACGVJG_002486"
     CDS             complement(1878..2273)
                     /locus_tag="ACGVJG_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558399.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            complement(2316..2567)
                     /locus_tag="ACGVJG_002487"
     CDS             complement(2316..2567)
                     /locus_tag="ACGVJG_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558400.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            complement(2567..2962)
                     /locus_tag="ACGVJG_002488"
     CDS             complement(2567..2962)
                     /locus_tag="ACGVJG_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558401.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTCSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            complement(3317..4318)
                     /gene="ccpA"
                     /locus_tag="ACGVJG_002489"
     CDS             complement(3317..4318)
                     /gene="ccpA"
                     /locus_tag="ACGVJG_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFJ6558402.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            4528..5631
                     /locus_tag="ACGVJG_002490"
     CDS             4528..5631
                     /locus_tag="ACGVJG_002490"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFJ6558403.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(5694..6296)
                     /locus_tag="ACGVJG_002491"
     CDS             complement(5694..6296)
                     /locus_tag="ACGVJG_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFJ6558404.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(6299..6739)
                     /locus_tag="ACGVJG_002492"
     CDS             complement(6299..6739)
                     /locus_tag="ACGVJG_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFJ6558405.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(6864..7802)
                     /gene="galU"
                     /locus_tag="ACGVJG_002493"
     CDS             complement(6864..7802)
                     /gene="galU"
                     /locus_tag="ACGVJG_002493"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFJ6558406.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            complement(7817..>8112)
                     /locus_tag="ACGVJG_002494"
     CDS             complement(7817..>8112)
                     /locus_tag="ACGVJG_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFJ6558407.1"
                     /translation="GDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLANMGMVVEGVNTT
                     QAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKENEFSLTIF"
BASE COUNT         2089 a         1701 c         1378 g         2944 t
ORIGIN      
        1 attttcacaa aatttttcta tataacttta ctaacgaaac tgttttaatt tcttttcttg
       61 tctttggaat aattcagtta gtttttctgc aaattgccct tttactgatc ctttcaagtg
      121 tacatgtcgg ttagaaaatt cttgagcagc ttcttctgca gtcatttggt agttttgatc
      181 tgtcaatcgt ttttctgccc aaatttgctg agatatcccc ataccaactg aacgtacatt
      241 tgatcgatgt agatttttct ctaattcttt tagtaattgc attaaaaatt cattcataag
      301 atgatttctc catagttatt tgtctgattt tttgacgata ggaacgttct agttcatctt
      361 gctgctgata gagttttcgt cgttctactt gtagggcttg ttgataatgc tcctgataat
      421 tcgaataact tcgtcttgct tgaaacagag gctcattatc taaagctaag ttctttaata
      481 catgatgcag tcgctgctcc tcttgctcca tcaatcgtaa tccttgattc ttaaagtgga
      541 tcaattcatc ttcttgagct tctaattgtc ttcgttcact tcgataatct ctttccaatt
      601 cttctagttt catctacttc tcctttaatt gaattgctgt gctaattctc gatctcgtgc
      661 cacgatttca ttgattttgt cttggatctc cttcgccaat gtatcaaagg cctcccccat
      721 acgtcttgcc ttattgattc gtagcagata gacttctttc gcagaatcga ctaagttttc
      781 ttttgtggcg cccacactag ctaatgcttc tatgatttcc tgttcagtca acgaaggggc
      841 tacaacacgt gcttgccgca agccttcttg ccattttttc tctagctcaa aaattcctct
      901 ttgattgatg cggataactt cttccatagc gcttttcatt tttgccgata acttttccac
      961 tactaaccta gcttgtgtgt catctaagta aatttgttca ttaactgtca gctggccgcc
     1021 acttaatcgt agttttttac gtatgttgtt cagttcttgc aactgatagg aaaacaactg
     1081 ggtcgcttgg ttttctttct gttgttttcg agctttaaca ttgttttcat tttcaggaat
     1141 aatcagcttt ccattttcat caaacttcca agaggttaaa ctgtgggaag caagcagatt
     1201 caagaatgct gtttccatac tgatccgaac ttcagcacct gtgccattac caccaaaatt
     1261 accgataaaa tcggcgtgat ttcggtaatt ggtgagcata ccgggatttt cttccaccca
     1321 tttttttgct tctggtgata agagatcata cgggtccggc ccgttgaagc cgacattttt
     1381 ccattgtttc tctgctgcta catataatgc taagaatcca cctaatgaat ggccggtagt
     1441 agaaatagca gcttctccat attccttttt tacttcatta gcaaatgcaa tcgctgaatt
     1501 gatttgcccc gctgttttca atccactaac ggataaaatc ttgtttgttg ttgctttttc
     1561 agaaaaactt gctcctggtc tattcccgta gagattcatg atacttcttg catccgtttc
     1621 caaatctttc caatcactta agttcgttcc tgcaaaagca atcactactt gagaaagatc
     1681 cactttaccg ttcttgattg gcgctacaga catcgcctgc atgccgtcca atgagtcttt
     1741 gatttgtagg atttgaaatt ccataccagc aaattcttta acagagttct ttttgtatcc
     1801 ctcattgtag cggggatatt tgggatctag ccaataaacc ttctcactaa tatcattgta
     1861 atgcttatcg tttattgtca tactaactcc tcctgtccgt tagaaaaaat tacttttact
     1921 ctttttatag tgactcctgt cttttgtacc gctctgttct caatgccaaa accaccaagt
     1981 tcatttctct ccttccaaaa actataatca aaataaacag atttcccttc attatttgtc
     2041 attgtcacaa gtgcaccata acttccggta acagggtttt ttttaaattc ttctaccttc
     2101 acttccctga tatccgcaaa ggtatttttc agaactttga ctgactgttt ttctactgcg
     2161 agtaaatctt tctgcctttg tgcttctctt ttgtccatat agactttccc tccgatccct
     2221 aatatgacga ttaataaaat ggctaagctg agttttctta ttttccttga caagaacgtg
     2281 cctcctttct ttttatgtaa gtattgacac cttcattatt taagtacgcg aaagtttgca
     2341 ggaatttttt tatttttgaa atttttctcc aatttgctat ggacgattgg cgctacagcc
     2401 atcgcctgca tgccgtcgaa ggagtttttg atttttagga ttttatacat agtgccgtta
     2461 attttaaaac ttaaattttc ttgcatttca ggtacatagt ctctatgttt aggatctagc
     2521 cagtaaacag catcacttaa ttcactatat tgtttgtcag taatcattaa atactttcct
     2581 catttccatt agagtaagtt actttaactt tattgagcgt atttccttct ttttgctttt
     2641 tttcatcaac tattccgtaa cttcctatct catttctttc tttccaaaaa ctataagaaa
     2701 aataaatcga ttgatcttgt ttattagtca ttagaataac tatccggtat gaacacgtca
     2761 tttcatttcg agcagatttt tcaatcttca cttccttgat atccgcaaag gtatttttca
     2821 gaactttgac ggactgtttt tctactgcga gtaaatcttt ctgtctttgc atttctcttt
     2881 tgtccatgta aaatttccct ccgattccta tagtaagaat ggtcaaaatg atgactatgg
     2941 tctttcgtct tttcttaggc aaatagaagt ctcctttcgg gttatatatg atggattgtt
     3001 tctcctttaa gataagtacg caatcgagcc tggatttttt tggatttttt agagttactt
     3061 atctactttt ctactttctt ttttatcacg ttttcatttt aaagtaattt tgatagtagt
     3121 gaacaaaatg tagcatctac gcacagattg tgtcgtatac gatggacgta aggtttgttt
     3181 ccgccatttt ctcttttttt aaagtcacaa aaaaaaaggg gttatgacaa aattttgtca
     3241 taaccccctt tctcgtataa acgttgttcc agaagttgac cacttttgga acaaccttta
     3301 aatgattgat cttttattat ttagttgatc ctttttcttc gattccataa ggcaagataa
     3361 tcgttttttc ttcgacttct tctttattca tcattttcgt caataggcgc atggataccg
     3421 ctcctaaatc gtataatggt tgtgtgatac ttgtcaaacg aggacgagct acttctgtca
     3481 gtaaagaatt gttgcttgtg atgatttcga attcttctgg tacttttacg ccgcggtcta
     3541 ataaaccatc caagatacca atggctaatt catcgtctga aacatagaca gctgttgcac
     3601 cgctattatg gatccggtca actagagaca gaccatcttt aaagttgtat tgtgcctcaa
     3661 aaatcaatcc ttcgctatat ggtagattgt tttcgcttaa tgcttcttta tagcctttta
     3721 gacggttttg accattgata ggatcaatca gcgcaccgct gacaaaagcg atttttttat
     3781 tgccgctttt tgctaattta tttactgcat cttttgttgc tgacacataa tcaatgttta
     3841 cgctacctac ttgttcatct ggatcaatgg aaccagctag tacaacaggc gtttttgaac
     3901 gtgaaaattc tccacggatt tcgtctgtga tatggtgacc cataaagatc acgccgtcca
     3961 cttgcttagc caataaatta ttcaatacat tgacttcttt ttgatcgttg ccatctgagt
     4021 ttgctaagat gatattgtat ttgtacattg ttgccacatc gtcgatacca cgtgctaaag
     4081 aggcaaagaa catattgctg acatcaggaa tgattacccc aactgttgtt gttttcttac
     4141 ttgccaagcc acgtgcgact gcatttggac gataatccaa gcgatcgata acttccaaga
     4201 ctttttttct tgttgctggt ttgacgtttg gattgccatt tacgacacgt gaaacagtag
     4261 ccatcgaaac atttgcctct ctcgctacat catagatagt gattgtttgc ttttccattt
     4321 gtgttctcct attcatattt tatatgtttt ctgaaaatac gtcttacatt tctaaaatag
     4381 gatatcaaat aatctcaaca attgcaaccg ttttatctct gttttcatga aaatttttac
     4441 ttttttcagt tttaagaacg ttggtctttt aatcaatcag tggtacaatt attgaataca
     4501 aaattaattt agaaagtagg ttgccaaatg aataacgtaa aaatcaatga actacgtcaa
     4561 tggatggcac aacaccaagt agaattggct tatatcagcg accctggcca catcgcttac
     4621 ttttccggat atgaaagtga tcctcacgag cgtgtactag ctctttttat ccctctggaa
     4681 caagatcctt tccttttcac tcctgctctg gaagttgaag atgctcaaaa cagcagctgg
     4741 gcgtacgatg tacgtggcta cttagacagc gaaaatcctt gggagatcat cggaaaagag
     4801 cttcgcgcac gctatgggca acctggtaat gtaggtattg aaaagaatgc tctgactgtc
     4861 gatcgcttcg atgctatctc tgatatcttg acaggtacag attcttacac cgatctaact
     4921 tctattatcc agcgtctgca actgatcaaa acggtagaag aaaaagacaa actgatcgaa
     4981 gctggtaaat gggccgatgt tgcctttgaa attggcttca aaacagtaaa agaaggcgta
     5041 actgaacaag caattatcgc agaaatcgaa taccaattaa aaaaacaagg tgtttcacaa
     5101 atgtctttcg acacattagt ccttgcagga gcaaatgctg caagccctca tggtacacca
     5161 ggaagcacga aggtttcacc aaatgaacta gtgttattcg atcttggcgt tatttggaat
     5221 ggctattgca gtgatgctac tcgtacagtt gcttatcaaa aaccaactga gttccaagaa
     5281 aaaatctata acatcactct tgaagcacag cttgccgcac aagaggcagt tcgcccaggt
     5341 gtgacagcag gtgaacttga tcaaatcgca cgaaacgtga tcaacagcta tggctacggc
     5401 gaatatttca atcaccgttt aggtcacgga atcggaacaa ccgtccacga attcccttct
     5461 cttgtagaag gaaacgactt agtgattgaa gaagggatgt gcttctctct tgaaccagga
     5521 atctatattc ctgaaaaagt aggggtacgt atcgaagatt gtgtgtatgt gacaagtgat
     5581 ggttgtgtgc cattcacgac tacaccaaaa gaattacttg ttttagagta atgaaaataa
     5641 aaaaggtgag agctggtccg ctctcacctt ttttattcta gaaatgagta atcttagtga
     5701 ttggtagtcg aatctgtcat tgtatctttc atttcttttt tagcttcttt tgcatcttct
     5761 tttgccattt tcaacatttc ttttgtgtca tctgcaactg gttttgcttc tgacattcct
     5821 tcattgaccg tctctcctgc tttatctgca gcatctttta catcgatcat gatatcttct
     5881 gaagtatctt tagcaatact acctaattcg tctgctggct ttttcagatc ttctgctgtt
     5941 tttttgaaac gattagataa atcagctgat tgttctttga ggtttaacaa gatatcatct
     6001 gattcacttc ctgcttcctc tgctgattca cttaccttct gcttaacatt gcctgctaga
     6061 tcccctgctt gttgagaaag accttgcgct ttttcttttg caatacctgt taactcagat
     6121 gatttttctt ttgccatatc tgtgtaatct gaagccatgt ccatcaaatc atctgcttga
     6181 gcagccaatt catcccgaag ttctttacca gatttgggtg ctaacaataa tgcggcgatt
     6241 gctgcggctg tacctccaac tactgcacct aataaaaatc cacctttttt agccattctt
     6301 attttcctcc tgtcgtttca gtatgttttt tgtttttaaa aaatttcatc gctgttccac
     6361 cgacttttcc ggcaacagtc gctttagctg tcgtttttcc gattccgccg actttcgtca
     6421 cgatgttttt actagaatga ttaagttccg atacactttc acttaaatca gcaactgctg
     6481 tgaacaatgg atcgatcgtt gcgacttttc cattgatatc caagagcagc tcattacttt
     6541 tgactagcaa gccttctact tgtctagata aaagatctac gtcttgtgtg acgatctcaa
     6601 ttgtcgtatt tgcttctgcg acagtctggt ctactttttc taaagttttg gaaacttgca
     6661 acaatacgcg aacgatgaaa acgacaagta tcgcaaaggc gattgctgcg atcaatcctg
     6721 caatttctcc tcctgacatg atcaaactcc cttcttgtct ctcttttcta taaggatagc
     6781 attttcgtgt tttttaaaca aataaaaacg agcttcaact actttttatt caattagccg
     6841 ctgaagctcg ttagaaaaaa agattatttt ttcttttctt ctttgatttc tgttattcct
     6901 tttggattat gattgatctt gcttccttct gcttctaatg ttttacctaa agacatgatg
     6961 tattcacgca aaggtgcccc tacttctgga tgtgtcagac catattcaat acttgttttc
     7021 ataaaaccaa atttgtcacc aacgtcataa cgtttacctg tgaatttacg ggcaaatacg
     7081 cgttgtgttt tattcaacgt atcgattgcg tcagttagtt ggatctcatt tccagcacct
     7141 ggttgttgtt ctgataatac atcaaagatt tctggagtca ataagtaacg cccaataata
     7201 gctaaatcac ttggtgcttt gcttggatct ggtttttcaa caaagttttt gacattgtat
     7261 aacccatctt ccagtacttc ccctgggttg atgattccgt attttgaagt ttcatcgtga
     7321 ggaacttgca taacggcaat cgtagaagcg tgtgtacgct catagtcatc gatcaattgc
     7381 tgtgtcaatg gtacttcgtc ttccattata tcatcgccta gcataacgac aaatggttca
     7441 tttccaataa aggctcttgc ttgaagaacg gcatgtccta aaccctttgg atgagattga
     7501 cggatgaaat gcaagttgac atctgtcgtt tcttctacta atttcaataa ttcggtcttt
     7561 cctttttcac ttaggtttgt ttctaattca atatttgaat caaagtgatc ttcgattggt
     7621 cgtttagctt ttcctgtaac gatcaagatg tcttcgatac ctgatttcaa cgcttcttca
     7681 acgatgaact ggattgttgg tttatcaaca attggcaaca tttcctttgc catagctttt
     7741 gttgctggta aaaatcttgt tcctaatcct gctgctggaa taactgcttt tttaactttc
     7801 atatacattc gctccttcaa aaaattgtta atgagaattc attttctttt tttccatcac
     7861 gtaacatgat ttcttttgcg gctgttttga tttctttttg ttcatacagc acttcataga
     7921 ttgtttgagt gatgggcatt tcgatttcca tcatttgggc tagttccatc gctgcttggg
     7981 tagtattcac accttcaacg accattccca tattggctag cacctcttct aggggctgcc
     8041 cttgtcctaa caagttgcct gcgcgccagt tcctagaatg aacacttgtg caagtaacga
     8101 ctagatcgcc ta
//
LOCUS       DAYLZO010000118         8106 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.120, whole genome shotgun sequence.
ACCESSION   DAYLZO010000118 DAYLZO010000000
VERSION     DAYLZO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.120"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..307)
                     /locus_tag="ACGVJG_002495"
     CDS             complement(<1..307)
                     /locus_tag="ACGVJG_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFJ6558408.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     "
     gene            950..1513
                     /locus_tag="ACGVJG_002496"
     CDS             950..1513
                     /locus_tag="ACGVJG_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFJ6558409.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1792..2079)
                     /locus_tag="ACGVJG_002497"
     CDS             complement(1792..2079)
                     /locus_tag="ACGVJG_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFJ6558410.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(2069..2398)
                     /locus_tag="ACGVJG_002498"
     CDS             complement(2069..2398)
                     /locus_tag="ACGVJG_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFJ6558411.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2703..3032
                     /locus_tag="ACGVJG_002499"
     CDS             2703..3032
                     /locus_tag="ACGVJG_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFJ6558412.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(3319..3804)
                     /gene="vanZ-A"
                     /locus_tag="ACGVJG_002500"
     CDS             complement(3319..3804)
                     /gene="vanZ-A"
                     /locus_tag="ACGVJG_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFJ6558413.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3957..4868)
                     /locus_tag="ACGVJG_002501"
     CDS             complement(3957..4868)
                     /locus_tag="ACGVJG_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFJ6558414.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(5296..5904)
                     /locus_tag="ACGVJG_002502"
     CDS             complement(5296..5904)
                     /locus_tag="ACGVJG_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFJ6558415.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5910..6941)
                     /gene="vanA"
                     /locus_tag="ACGVJG_002503"
     CDS             complement(5910..6941)
                     /gene="vanA"
                     /locus_tag="ACGVJG_002503"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFJ6558416.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6934..7902)
                     /locus_tag="ACGVJG_002504"
     CDS             complement(6934..7902)
                     /locus_tag="ACGVJG_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFJ6558417.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2456 a         1608 c         1361 g         2681 t
ORIGIN      
        1 aagagaaggt tcgctctgca cggccaataa tgacgtctgg ggtccaacct tcagttgatc
       61 ttttttcaat gtattctttt tcctcagcag gaaaaatgat ttcggttctc ccacaacgtc
      121 gcttattttc tttgtattgt tcaaagtatt caagtgctgt tccaccacat ttgagaaagt
      181 tatagacatt gtaaattgtt tggcgtccac gtttaagctg cttcgcaacg atagcaaccg
      241 gagtttcttg atgaaaatat gcttctatca ttacaagctc gtttggtgta agatgggtat
      301 aagtcattta tgttcactct ccttgtatgc tttagcgggt attacaattt gagtgtaaca
      361 taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagttaa aactttttta
      421 gcagatttta aagtttcgtc aaaactaata tattctggta gctgagcatt caaatagctt
      481 tcagacttca gaagaaaatt acgagcattt ttatagtcca tctctaataa agttattctt
      541 tttttatcca atacattcag ctcaattcct tataatattc tattccttag tatctcaaca
      601 ttatttactt tataaaacga ataaattttg tagttctaat tcaaatattg catgttatat
      661 ctaatatggg agcattttat aattttcatt gtgataaact cgttattaac tttaggatac
      721 actagttagg attaaattag gtatctagaa atttacttaa gatttttttg tttatttcgt
      781 atacacactc gtatcaaaaa caatcgatta tgagacaaaa tgaaatgacc tcttttatta
      841 aataaaagag gtcatatttt gtatcataac ttgttgcatt aactatgtat ctataactcc
      901 cttttatgat acaatcaagc cagaattaaa gtacgaaagg aagaaaagca tgagtggata
      961 catttatggt tatgcccgag tgagtacacg ttcacaagaa ctaaatcgtc aattagactt
     1021 actcaatgaa caaaattgta atgaaatcct aactgagaaa atgacgggaa ccaaagcggt
     1081 tcgtccccaa ctaaatcgtt taaaagataa aattcgtccg ggagatacgg taattgtaga
     1141 aagtttttct cgattgggtc gtagtacaaa ggatttgatt gaccttgtta cctattttga
     1201 agaacaccat gttaagctgg tgagcttgaa agaaaacttc gataccagca cgcctcacgg
     1261 acgcttaatg atgactgtct tccaagcatt tagtcagttc gaacgtgatt taattgttga
     1321 acgtaccaaa gaaggtttga aaagtgcacg tgcgagaggt cgtaaaggtg gccgtccacg
     1381 agtgaaccag aaagatgttg atcgagcagt aaaattgtat aaaagtcagg tttatagcgt
     1441 aaaagaaatt acggaaatga ctggtattag caaggcaaca ctctaccgct acttaaaaga
     1501 caatagggaa taatctattt taatctttag gaatctataa tatttatggg agaattagag
     1561 tgattttttt attattacta aaaaacaata taaaataacg cattttaagc cctctttgtg
     1621 cataaggatg aagcttttaa tttgaaagag gcttaaaatg cgttttaatt ttttctattt
     1681 ttcataaata tataggttag tggtttaaaa ttatagggta ttacaactct cagctgttaa
     1741 aaattatctt ttaaagtgtc gtctatcctc tcaagttgaa acattgggtt cttacctaat
     1801 cttttttaat tgttcataaa aattttcatg cgttccacat agtaagatag gtatcactgc
     1861 attatcttta atttcatatg caacgcgata tgtcgtacct gcatacttga atccaatagc
     1921 ccaaattcct gataaatcac ccgttttttg ttctcccgaa tgaggacgaa ctgctatctc
     1981 atcataaatt atcgttaaaa attgttcttt taatgttttc tcttttaatt tttttagata
     2041 ctttattacg tgtttagaag gtagaatctc ataagccaat ttctgctgcc aactcctctt
     2101 ttgtcatcgg tttagaattt gcaacagtat cttcagcaat agatttaaat gcagaagtta
     2161 acgcattttt tctatttcta aattctttta agatctcttg tttgctataa ccttctgaaa
     2221 gaatatcagc caaaatatct tcactaaaat caaaaaattc ttttttaggc tctacaaact
     2281 cataaaggat accattctct acaagcttag cttctacttc aacaccattg ggcacatcga
     2341 attctttagg gaccgttaac ataatagaat ttccttgttt tcttgttttt gtaatcatat
     2401 tatttcctcc attattttac atttcgtaat tacttagtaa ttacattgta cgctattttt
     2461 agtagaaata caaaataata aaactccttt agaacaattt tcaaaggagt tttatttgat
     2521 taggaaatta tgtagaataa taatatgttt ttaacttgga atcaaaaacg cacttgcaag
     2581 taagtttact tcaatacatg aaattgctta taatagtgta ttggaattaa ggtagattga
     2641 caaaagagtg atcaaatagg tatacttcat tcaaataatc gtttgaatgt gaggtgagat
     2701 gaatgaaaaa agtttgccaa gtgactgtcg tacataaaga aaaagtaaat ggaataaaaa
     2761 ataaattgga tcaaaaagat ttttcaaact tacttgtgtt agggaaatgt ttcagcgatt
     2821 tttctagaat taaaattttc tatgctttag aaacctataa ggaaatgtgc gtctgtgatt
     2881 tagcagaaat acttactgct agtgtcgcta caacgtcaca ccatttacgt tttttaaaaa
     2941 aacatggaat ggcaaaatca cgtcaagatg gaaaagtagt ttattactct ttagcgaatg
     3001 aagacatcct ttccgctatt cgtgtttttt aaagttatca gaaaatctat ctatgaaatc
     3061 ttagtttggg ggtaccgtca ggaaaatgcg gatttacaac gttaaggaaa tttattaaag
     3121 agaccaattc ctcagcacta aaattgataa ttgggctttc tttgttacta tagtgttagg
     3181 taagcttttt actttatcac cggttttccc ctcacttcac acctacgggc gagtttcccc
     3241 acatcccgtt ttccatctaa tccaattcat tcaatatctt tatgtgtatg aaagatcaga
     3301 ttgcttgatt tagacatctt acacgtaatt tattcttaaa tgggtacggt aaacgagcaa
     3361 taatacgagc aaaagtatac ctacaaaaat aataactctg tctaattttt tttgattcat
     3421 atgcttattg cttaaaccat ataatttcag tccaagaaag cctccaacag tatttgtaat
     3481 tacatctgtt atgtctgtcg ctccaatagc gaagataaat tgaattattt caaaagtaag
     3541 acttaaaacc agtacaaaag caaacttagg taaaaatccg atttctttaa aattgacatt
     3601 caaaagcaag ccaaatggaa taaagattat aacattatct atcatctctc tgaaattccc
     3661 agtagcagta aatggagtca agttaagact tctttgatga taattaaata ctgataaaat
     3721 attgtattgt aatttgaata acactaacca gattagtgtc actaaatata aagctagcaa
     3781 tcctctagat aatatttttc ccaatataat ttcctctaat tcttttgttt aagttgtatc
     3841 attagtatag caatgtccta gttaacgtgg gttttaaatt aaaaagctaa cgttacccca
     3901 aaagtgctag tacaataatg ctgttcatta atttcgttgg tttcattccg ccatccttac
     3961 ctccttgaat tagtatgtgt tgatccggga aacactgtca caattacacc gtctatattg
     4021 tttcctgata tctcataacg aagattagtc ggcacatgaa tggtggtatt tttagtaaca
     4081 ggataataaa agatctcata tttttcccca tttacactaa cagaaatggt tttttcttct
     4141 tttaggtaat ccatatattc ctcgagaacg aaattctttt ctttcataat cgcactatgt
     4201 ggtaaaccaa catagcgaat atgccatggt tcatattgaa ttcctgttaa ctctgttttg
     4261 tcctctggat aacgtaaaat gaacccgtat ttccaagcat tttcttctat ccactttcct
     4321 tcaggggctc gttccatttt cgtcaagctt gatcctacat ctagtgataa acctgaatta
     4381 tgctcactat aacctgctgg taaggcatac tcagccccca tttcttggta aagcacactt
     4441 tgctcatcaa agtctcgata gccactatta ataataaaat gactaacgcc accctttaca
     4501 gcatcattga ccatctctga aaatttttgt gctatttctt ttgacatata aatattacta
     4561 tcaagcaacc cgtatccatt tattaattcg tcatgtttag ataaattcac gatatctgac
     4621 ttcacacttt cttggcgaac aggatattta ctattgatta atagcagatt tccttgataa
     4681 acctgttctt ctgtaattgt tttctcttgg gtattttcag aagtcccact attttctaaa
     4741 tgttctttgg gattttgatc ataattttga aattcgactt tttcctgaga aaacagtgct
     4801 tcattaacgt agtcataacc taagtatatt aagaataaca ataacaataa aaaaaacaac
     4861 ttcttcattt tcagtctcct ccttaactat atagttggaa gtatgaaata tttctagaat
     4921 cttcccctta aacggcacaa tctcgtttga gattgtgccg ttttgccccg taaattacag
     4981 tcaattattt cgccctactg gatttactat tatcaccaat gtagatgaat tttcataagt
     5041 gatacccatg ctgaatttaa cgaggctgaa ctgcctgttg acaatgggcg gtgtgaaata
     5101 ccacccctct atgcgatcag tcttattatc aggctatctt gccgcagcgc acgcagataa
     5161 tgaccgcaat atcgctctgc cttacatctt caatactatc tactatatat aaattcccta
     5221 agaaccagtt acatacgtcg ggtttcctgc cgaaagagtt agcttatata gtttgtccgt
     5281 gcaacggtta aaagtttatt taacggggaa atcaaaatag ctattggggt atggttcgtc
     5341 tcttaataca tagtgccacc attcgaggct atatgcttca aacccactgt tttccatgat
     5401 ggagcgcaaa cgtctgcgat tttgcgcttc attgcatgat attccatttg ccgcatgatg
     5461 agagcgttca tccataaaat caaatcggct ccccattggt acaagctcac ccgtgtctaa
     5521 tcgataaagc gtaagatcaa tggcactgcc gcggctatgg cttgattttg aagccacgta
     5581 tccttttgaa atcatctcag ttcggtcaat attgggataa taactttcct ttgtcaggtt
     5641 attttccggc tgtgcagccc attgcataaa acagtttaca gcacgattag gacggtaacc
     5701 gtcccataga agcaatccgt acccttgggt agcagccagt tcttttgcct tcaaaagcga
     5761 ttcagccaac tcgtatgtcc ctacaatgcg atttacttca taaccgtcaa ccggttttcc
     5821 ggtgaaatta tcccaagtgg catatttagc gtcccaacga acaccgtgta ctatttcatc
     5881 taaaaaagta aatcctattt ccatgcttat caccccttta acgctaatac gatcaagcgg
     5941 tcaatcagtt cgggaagtgc aatacctgca gcggccatca tacggggata acgactgtat
     6001 gacgtgaaac cgggcagagt attgacttcg ttcagtacaa tgcggccgtt atcttgtaaa
     6061 aacatatcca cacgggctag acctctacag ccgagcgctt tatatatttt ttttgccgtt
     6121 tcctgtatcc gtcctcgctc ctctgctgaa aggtctgcgg gaacggttat aactgcgttt
     6181 tcagagcctt tttccggctc gacttcctga tgaatacgaa agattccgta ctgcagcctg
     6241 atttggtcca cctcgccaac agctaacgcg gcactgtttc ccaataccgc acaaccgacc
     6301 tcacagcccg aaacagcctg ctcaattaag attttgctgt catattgtct tgccgattca
     6361 attgcgtagt ccaattcgtc cgcgctattg acttttttca caccgaagga tgagcctgaa
     6421 cgcgccggct taacaaaaac aggataggta aacgtagctg ccaccggcct atcatcttta
     6481 ttaataaccc aaaaggcggg agtagctatc ccagcatttt tcgcaacgat gtatgtcaac
     6541 gatttgtcca tacaaattgc tgagctttga atatcgcagc ctacaaaagg gataccggac
     6601 aattcaaaca gaccttgtat ggatccatct tcacctgact tgccatgcaa agctgaaaat
     6661 gctacatcaa catggttgat ttcatattca tggttctttt taacaagtaa tccgtgcatt
     6721 tttttatccg gcgagagtac agctgaatag caattgtcgt tttcccattc cgcgcaaggt
     6781 ttttcgcaca ttttccatac accagatttc gtaattccaa tgtataacgg ctcgtatttt
     6841 tctttattaa tgttagcggc tatctctatt gcagatttta ccgatacgtc atgctcctct
     6901 gagcaacccc caaacagtat tgcaactttt attctattca tgctcctgtc tcctttcaaa
     6961 atccaaacag tttttaatgg ttttttcaac ggtatcacgc aacgcttgct cggtataata
     7021 ggccgtatgc ggtgtgatta tcacgttagg cattctttga agtttaagta aaaattgatt
     7081 atcaattggt ttttgggtgc aatcagagta gaaaaactct tcctctcctt ccaatacatc
     7141 caatgcggca ccgcccagtt tcccgttttc taatgcttta accaactcat aggtatctac
     7201 aagtggaccg cgcccagtat tgataagaaa tgctccttgc ttcattctct gtatttgttc
     7261 gtggctgata atatagtgcg tatccgtatt gagcggcaca tgaagcgtaa cgatatcgct
     7321 attttgcagc aactcatcaa acggtacata gtttacctct atacttcggc tgcgactata
     7381 agccaacact ttacatccaa atcctcgcag ccgctcaata accgctttgc ctatctggcc
     7441 cgttcccacc acaccaactg tcatgtcgct gagtaccttg ccacggtcgc tgtccaacct
     7501 gaaatcatgt ttttccacag agcgcacaat cgattttacg ttgcgtactg ccataagaat
     7561 tagcatcata gtataatcgg caacgctatc cggcgagtac gccacattgt cgacagtgat
     7621 gcccattctc ttagcagcag ttgtatctat atgattgcag ccgatgcttc gggtagaaat
     7681 atatttcaca ccggctctct tcagcgcaag aagaatagag gcggaaatct ctgatttatg
     7741 tcccacactg atacattgat tgaaaggcgc ggatttggcg ttggattccg acacgttggc
     7801 gttaattatc gttgccataa cgccaaagcg aggcgaaaga gcatggaatg catctgcctc
     7861 atcctgctca catccataaa cagtaatgcc gatgttattc ataattaaga ccaacccttt
     7921 ctgtgaaagg caccaaataa aacggggatg ccaatggtaa tcttgtcaaa tctttctctg
     7981 aagaacgaaa acggctcgtt cttacggtat taagtctacc gtgtaagaac gagccgtttc
     8041 ttaatattaa attgttaatt tcctaagaaa aagtaaagat aaccttgaga ttttcctaaa
     8101 aaatta
//
LOCUS       DAYLZO010000119         7884 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.121, whole genome shotgun sequence.
ACCESSION   DAYLZO010000119 DAYLZO010000000
VERSION     DAYLZO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.121"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..614)
                     /locus_tag="ACGVJG_002505"
     CDS             complement(<1..614)
                     /locus_tag="ACGVJG_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFJ6558418.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVA"
     gene            complement(627..1544)
                     /locus_tag="ACGVJG_002506"
     CDS             complement(627..1544)
                     /locus_tag="ACGVJG_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFJ6558419.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            1972..2535
                     /gene="efp"
                     /locus_tag="ACGVJG_002507"
     CDS             1972..2535
                     /gene="efp"
                     /locus_tag="ACGVJG_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFJ6558420.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2636..2806
                     /locus_tag="ACGVJG_002508"
     CDS             2636..2806
                     /locus_tag="ACGVJG_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558421.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            3021..4280
                     /gene="lysA"
                     /locus_tag="ACGVJG_002509"
     CDS             3021..4280
                     /gene="lysA"
                     /locus_tag="ACGVJG_002509"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFJ6558422.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            4267..5067
                     /gene="dapF"
                     /locus_tag="ACGVJG_002510"
     CDS             4267..5067
                     /gene="dapF"
                     /locus_tag="ACGVJG_002510"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFJ6558423.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            5070..5951
                     /gene="dapA"
                     /locus_tag="ACGVJG_002511"
     CDS             5070..5951
                     /gene="dapA"
                     /locus_tag="ACGVJG_002511"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFJ6558424.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5948..6664
                     /gene="dapB"
                     /locus_tag="ACGVJG_002512"
     CDS             5948..6664
                     /gene="dapB"
                     /locus_tag="ACGVJG_002512"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFJ6558425.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6670..7365
                     /gene="dapD"
                     /locus_tag="ACGVJG_002513"
     CDS             6670..7365
                     /gene="dapD"
                     /locus_tag="ACGVJG_002513"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFJ6558426.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            7381..>7884
                     /locus_tag="ACGVJG_002514"
     CDS             7381..>7884
                     /locus_tag="ACGVJG_002514"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFJ6558427.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDL"
BASE COUNT         2613 a         1373 c         1694 g         2204 t
ORIGIN      
        1 gctactacat cgctgtgtcc gcctagatat ttggtaccgc tatggacaac gatatcagaa
       61 ccgagatcta aaggtctttg aaaataagga gtagcaaatg tattgtcagc aataactatc
      121 aatccatgtt gatgagccag agtggcactt ttttctaaat cagtaatctt tagaagcgga
      181 ttgctaggtg tttcaagata aagtgctttt gtattgggtt tgatggattg ttctatttta
      241 tcaagattac tcgtatctat gatcgtgtac tcgagaccat ttttagtaag aactttgtca
      301 aatagacgga atgttcctcc atagacatca tctcctaata agatatgatc gccagcttga
      361 aaaagagaaa aaacggcatg aatcccagaa agaccagaag aaaaagcgaa tcctcgaaca
      421 ccgccttcca aatcagcaat cagttcttcc aatgcaaaac gagtaggatt accagatctt
      481 gaatattcat attgtttagg ctggccgacg ccatcttgcc gataagtgga tgtttgataa
      541 ataggtacac tgacagctcc tgttgtggga tcttcactga tgcctccgtg gatcaatttt
      601 gtttgaatgt gcatgtttct ttcctcctat aagtaaatgt tttttgataa atagcggtct
      661 gctgcgtctg gaaaaatcgt aacgacacga tgaccaggtg gtaaacgacg aatttctctt
      721 aatgctgctg caaaagccgc accactcgaa cttccaacta atagtccttg ttctcgtgct
      781 aactgcctag tatagttaaa cccttctaca tcagagattg tctcgatctg attgattgaa
      841 aggggagaaa gaaacggcgg aatgaattct acaccgatac cctcaatttc gtgaggggct
      901 ggatcaccac catttaacac agagccctct ggttctacac cgattattcg aatgtcggga
      961 tatttttctt tcaaaaaggt agaagttcca gcaaaggttc caccactccc aattcctgct
     1021 acaaaactat ggatattttc tttgatttca tgaaagattt ctggccctaa cgtatgatag
     1081 taagcagcag gattatcttt attttcaaat tgaagaggca gatagctgtt tgatatctcc
     1141 tctgcaagtt ttttactttt gctgatggca ccgatgattc cttgctcact aggagaatgg
     1201 atgatctttg ctcccaatgc tttcatcaat tgttgttttt ctaagctaaa cttctctgga
     1261 acaacaaaaa ccgttcttaa tttgtatgtg agtgcggcca gagcgatccc aattcctgta
     1321 tttccggcag ttggttcgat gatcgtcgtt ttttcattga tttttccttg ggcaaatcct
     1381 tctttgatca aataactgcc caatctatct ttgatacttc ctccaggatt taaatattct
     1441 agttttgcat aaatagcaga acctttaggc atagaatagt gctgattgtc aaagcgataa
     1501 agcggtgtgg aaccaatagc ttctgtaatt gtcgagataa tcatgtgggc ctcctaaaaa
     1561 aagataataa aaaaaggagc tgactattaa atcagctcct tgaaagacat agagaaaaat
     1621 tgagtagata aaaagataag ttacctggta ctcattctat gaagggcaat taatagacat
     1681 gacaaatagt atgctgcttt tatgcaccat acaacagcaa cgattcaatt gtttttggtt
     1741 gctgactgtc atgtgtatcc ctccaagtaa aatttttatt aacgatagca tgaaaaaaaa
     1801 taaaagtcaa aggcttgaaa gaaaaaaata tttcgatagc atcgtttacc cgtgtttatt
     1861 tttctgacaa ataggcatga gaccttaaaa gatagagaat actctagttt tctggccggt
     1921 tgtctgatac aataaagttg attatgataa agaaaagaag gagcaaaaat aatgatctca
     1981 gcaagtgatt taagagccgg tatgactttc gtacaagacg gcaaactgat taaagttctg
     2041 gatgcaagcc accataaacc tggtaaagga aatacagtta tgcgtatgaa actgaaagat
     2101 gtccgttcag gtgcaacata cgacacaacg ttcagaccag aagaaaaatt tgaaaaagca
     2161 catatcgaca caaaacctgt acaatatttg tacacgatgg atgatacagc tttcttcatg
     2221 gatttggaaa catatgagca atatgaaatt cctgtagaaa cagtagaaga agaaatgaaa
     2281 tatatcctag aaaacatgga agtaaaaatc caatttttcg gtagcgaagt catcggcgtt
     2341 caattaccaa caactgtcgt attacgtgtt gtggaaacac aaccttccat caaaggtgca
     2401 acagtcactg gttcaggtaa gccagcgaca atggaaactg gcttagtagt caatgttcct
     2461 gacttcatcg aaacagatga attgttggaa atcaatacag cagaaggttc ttatgtaaaa
     2521 cgtgcgtcaa aataattcgc taacggattg ataagaattt gaaaacagag cttgacctct
     2581 tgaaaggcag atcatcggat ctgcctttca agaggttttt tgtggaatca gcaaattgaa
     2641 aaataaaaaa gtatttgatt ttatttatat tttgaaagat gaatggcaaa agctagagaa
     2701 agaaaaaact gcacttattt atgaagaaat tgaaatgatt cataaaattg aaaatatccg
     2761 aactattccg aactatattg aagcgaaaat attttttcat cactaaatag tgttgaattt
     2821 atgtttttat gtataaatag cttctaatag ttcgggtatt ttgtttttat aaccattttt
     2881 attcttattt ttaaaaaaat atttcgaaat atactagaca attcagaaaa ttttgtttaa
     2941 tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa
     3001 gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca gcaagcacat
     3061 tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg agacaaacga
     3121 tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg ctttacgcct
     3181 ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga ttcgggctag
     3241 atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct agagatacta
     3301 tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt gaaaatgatt
     3361 tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa ttaagccaac
     3421 gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa gcacatactc
     3481 atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat gaaagtaaag
     3541 agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta gaaggatttc
     3601 atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa atcgacaaat
     3661 tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc ggagggtttg
     3721 ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta aaaaaactga
     3781 ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg
     3841 aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt ggtttcatca
     3901 aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat aatatccgtc
     3961 cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa
     4021 cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag
     4081 cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg ggagcttatg
     4141 gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt tttgtcaata
     4201 atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta aggggggaag
     4261 tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt attagattac
     4321 gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga aaaatatgat
     4381 atagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta caacaaagat
     4441 ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata cgtaaatgat
     4501 cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac aagaaaaatt
     4561 acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc agatgattct
     4621 ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat agagatcaat
     4681 ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcataccgt tgtttttgtt
     4741 gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca tcatcctttg
     4801 ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga attgatcgtc
     4861 cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg ctgtgcctct
     4921 tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt tcacttggaa
     4981 caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc agcagttcat
     5041 gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca
     5101 cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg atccaattcc
     5161 atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa tcttctacgc
     5221 tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg gctggccgtg
     5281 tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa aaagcgaagc
     5341 attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac aacaaaacaa
     5401 gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg attccgctta
     5461 ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta ttagtaaatt
     5521 tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg gcttatgtga
     5581 tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat gatgatttga
     5641 tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg aatatccaac
     5701 ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag caagcaaaag
     5761 aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc aatcctattc
     5821 ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc ctgcctttag
     5881 tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa gaaaggacgg
     5941 ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag tcgaagaagt
     6001 tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag aagcaactac
     6061 gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag ataatttaga
     6121 gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca cgacaggtta
     6181 tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg tgatgtatag
     6241 tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag cagctgcaat
     6301 gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa aagatgcacc
     6361 atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt
     6421 atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc
     6481 aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa tatttacgat
     6541 caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag cagcggactg
     6601 gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg acaaaggggt
     6661 gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc taaaaagacg
     6721 cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt
     6781 ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc ggttttagaa
     6841 gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt
     6901 cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat tatcagagat
     6961 caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa tattggtgca
     7021 gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg agcgacagtg
     7081 ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga accagcatcg
     7141 gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt gattgttgaa
     7201 ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg
     7261 gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag tgatgatcag
     7321 acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag atggagaaaa
     7381 atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca aacgatgctc
     7441 aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc ttcaaaacgt
     7501 tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga attgacagaa
     7561 acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt atccaaagag
     7621 tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc agcatggcgg
     7681 gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta tacattgaat
     7741 cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct taaagcttta
     7801 caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt ttctggttcc
     7861 gggcaaaaag gcattgaaga tctt
//
LOCUS       DAYLZO010000120         7840 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.122, whole genome shotgun sequence.
ACCESSION   DAYLZO010000120 DAYLZO010000000
VERSION     DAYLZO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.122"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..934)
                     /locus_tag="ACGVJG_002515"
     CDS             complement(<1..934)
                     /locus_tag="ACGVJG_002515"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HFJ6558428.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNS"
     gene            1130..2875
                     /locus_tag="ACGVJG_002516"
     CDS             1130..2875
                     /locus_tag="ACGVJG_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFJ6558429.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(3155..3592)
                     /locus_tag="ACGVJG_002517"
     CDS             complement(3155..3592)
                     /locus_tag="ACGVJG_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFJ6558430.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(3576..4949)
                     /locus_tag="ACGVJG_002518"
     CDS             complement(3576..4949)
                     /locus_tag="ACGVJG_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558431.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(5271..5759)
                     /locus_tag="ACGVJG_002519"
     CDS             complement(5271..5759)
                     /locus_tag="ACGVJG_002519"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6558432.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(5781..6383)
                     /locus_tag="ACGVJG_002520"
     CDS             complement(5781..6383)
                     /locus_tag="ACGVJG_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFJ6558433.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            6482..7821
                     /locus_tag="ACGVJG_002521"
     CDS             join(6482..6923,6923..7821)
                     /locus_tag="ACGVJG_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFJ6558434.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         2642 a         1554 c         1363 g         2281 t
ORIGIN      
        1 tagagtttaa ttcattcagg aataattcat ttttatttgt caaaaagaaa tcgcaccggc
       61 tcaatccgct tccacctaac atcgtgtaag ctaacttcgc gtactcttgc gctttttgat
      121 aaacttcttc tggcacttcg gctggaatct gcatttcgat tttattattg atatattttg
      181 cttcataatc atagaatgct acgtctttta cgacttcacc aggcaaagtc gtccgaacgt
      241 cttcatttcc taatacagca acttcgattt cgcgcgcttc aattccttgt tcaacgattg
      301 ctcgagaatc atactgatag gctgttgcta aagcattttg cagctcttct cggttttctg
      361 cttttgtaat gccgacacta gaacccatat tcgccggttt gacaaacatc ggataaagca
      421 aagaaccttc acattgatca aatacttttt taggattttc tttccattga ttcttaagta
      481 ctggtacata aggaacttgc ggcacaccag cagcttgtaa aatatacttg gtcatgattt
      541 tatccatggc acatgcactg gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct
      601 ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc atgtaaaact ggaaaaacga
      661 tggctccttc ttctttgatt tcgcccggat tgatcacttt tcctgtaaag ccttcctctg
      721 tctgtccact tgggtcccat gaaagatgca agacatcttt gctggcaggt ttttctgtta
      781 gtaatggacc tttgacccat tgtccttctt ttgtaataaa tacgagttga acttggtaat
      841 aattataata aatggcattt aaaactgaaa atgcggaaag aatggacact tcatgctctg
      901 cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt gtcctcctgt attctaaatc
      961 gttcagtgat ttactctcat attttagcac aaaacaaaaa taaacgaata acttatttat
     1021 aaaaaaacga gccagcttct aaaagaacga gcgtcattta ttttttgtca gctagattat
     1081 ttgtcccgtt ttttataaaa agttatgata aagaaaagga gggatgatga tggcgcaaac
     1141 aaatccacgc agccgcttgg cagaaatcat tcgagtgttc atcaattaca atgtagtgcc
     1201 aaactttgta cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc
     1261 caccttcatc aagataggac agatgctttc tgttcgagaa gatttgcttt cttctgcctt
     1321 cactcaaaca ttcaaaacat tacaagatag tgtacctagc gacacatttt caacggtcaa
     1381 aaagacgatc gaaacagaac tttctctttc attatcagat atattcgatg atttttcaaa
     1441 atcgcctttt gcctctgctt ctatggggca ggctcatcgt gccaagctga agaacggtga
     1501 cgcggtagtc gttaagatcc aacaccctaa catcgccgaa gagatccgac ttgatcttca
     1561 actatttgaa cgagcgatcc ctttgatcaa atacattcct gaaactagcg tagttgatct
     1621 aaaaggggta ctccaagaag taaaacgttc cttgatcaat gaaatggact tcttaaaaga
     1681 aagtcaaaat ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga tccgcagccc
     1741 caagatttat gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa
     1801 aaacttgaat catttaatga acgccgaaaa caaaactgaa acttttatca ctggaattca
     1861 aaacaagcag ctgaaacaag aagttgcaaa gcttttagtt gagaacttta tgaaacaggt
     1921 gtttgatgat ggttttttcc atgctgatcc tcatccaggg aatctgctat tccatgtact
     1981 aacaaaagaa gaacagacac aagctagccg aaaaactgaa acagtccatg aaaaagagtt
     2041 cggttccttt gcttttcgag cctccacttc tgcagaagac cctgttgcgc catataccat
     2101 caactacatc gattttggta tgatggggca tctttctgca ggacttaggc aaaaactcac
     2161 acaagccgtt ttagcgctgt atacgaaaga tgcttatcga atcgaaaaag cggtccttcg
     2221 actttgtcag caagaaggct cgtttgatga aagtcggttc caccaagaac tgacaagttt
     2281 tcttgaacag tattatgatt ctcctattga tgaaatcaac ttgcaagaag tattcacaca
     2341 tgtcgtgacg atttgtcatc aaaataatct gcagttcgat cgtgatatca cgcttttgct
     2401 gaaagcattt gggacattag aaggggtgat ccgcgtcctc gatcctgaag tttccctcat
     2461 ggaagtcgct tcgccttttg ctcaacatta ttttctcact catctggatg tagaagatac
     2521 attgaaacaa agcggattag acttattgga agggatgaag gctgcgccta agatccctca
     2581 acagctccac catctattag aaatgtggac ttcgggacaa ggaaaagtca atttggaatt
     2641 gaaaaagcaa gacaaattat tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg
     2701 tatgatttta gctgcattga tcgtagggtc ctccctgctt gtccaagcag cacctgtaga
     2761 aaacgcagag gtcgtttcct tattagggat atttacgtat gctattgccg cattcgtcat
     2821 tatttttttg gcaatcgatg cattgatcca aatgtataag aagaggaaaa agtagaaaaa
     2881 ggttgtgaca gaaagaatca gcttcaagaa ataagaaggg gttttacata aattgtttct
     2941 caaatttttg tgaaaattcg gcttattttt cgaagaggct acttctgaaa caccgagaat
     3001 aaaaaatgtg aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa gggaacatgg
     3061 aacacattta tcctgtttcc ctgttccctt tttgatgtat tcgagcataa ataaaatacc
     3121 ttcgttacct actatcttct gtacttggta aaccttagtc aattgccttt tttaacgctt
     3181 gacgaaaaga catatgatct ttctccgtaa agggttttgg accctttgtc cttatgccag
     3241 cttttcttaa ctttccacta tgataacgtt gttctagaag cgtgccgata ttcgcttcgt
     3301 caaattcttt tccagagtga tggaacactc gcacaccctt cggcaataca agtgaagcta
     3361 aaccataatc ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg attttataat
     3421 ccgcactatc agctccgcgg tctacgtaga cgatttggac atgattagcg tattctttgg
     3481 tcgtataatg gtccacactg gtcacgataa ccacgtcaag accataggat tctcctaatt
     3541 ttatgacatc ttcttttact ggagaaccat ctccatcaat gaatagcctc attctttttc
     3601 gccttcttcc ataaatgata aacagttact aatccgctca gtaaaagacc aacattgatc
     3661 cattttgcca agataagcag ttgtacactt tctatcggca ctgattcatg gttcagcgtg
     3721 atcaataata gatggattgg ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc
     3781 aataaaaact ggtggatcac actcccttta ttttgagtag tatacttgac aacgatccgg
     3841 cgatccattg gaatagctgt atcagtttga taatattcct tatttagcca gctgcctgtt
     3901 actacgtgcc agaaactaag cagaaagaca cctatcgcaa aaccatttgg aatcaagagg
     3961 accaataaag aagaactcag tccaagcaat tgcatagcca atggagacca tgttaaacgt
     4021 ttcaaaatcc atggtccgac tatttttgct gcaatcaagc ctgctgcata aggcaaatac
     4081 ataaaaatcg aaagtttttc taatccgtac agtatgccaa cataaaaact gctaaataaa
     4141 aaaaggaaat tccctagcat tccatttaga aaagtaaaca gatttagcca gcttggcaac
     4201 ttccattcct tcttctggtg tcctaaaaca aacacgaaca agatgaacag cagcaagaaa
     4261 ccataaatag caagatcaaa aagcctttca tcgaataaca atcgggcatt tctcaaaaga
     4321 aacaataagc caaagaatat caaaaataaa acaagctctc gcgtagctac cagatttctt
     4381 ttgatgtctt tgaaatccag ttcgtaacgc ggataatgac taaccgtatg gtaggccatg
     4441 acgtaaaaaa gtgtataaat agtaaagctt gctgcaatct gagtcggtgc aggcaactca
     4501 atcgcttgat acaggagaaa tagccagata gctgcaaata agtacttttt cccatccata
     4561 ttgataaaac cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat
     4621 gctgcactca gccctagcaa aacacttgaa aaataaaata aaggaaaata aaatacgcct
     4681 aatattccaa gcaatgaacc agcaccacca atcagcagag cgatcatcag caacgtgtaa
     4741 ctatctagac caaatcgtag actgttgact aaaaatagtc cagtcatccg aaaggcataa
     4801 aacagcgtaa atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa
     4861 agataaggaa taaaaataac cgtgttactt aagataaagg gcgttgtttg tgtaaaatta
     4921 cgcccaaatc gtttgagttt ttcgaacatg acaatggctc cttaaacagt cttataacta
     4981 atctgctctt cgattttagc attaaagggg aaataaacca attttacaga atatattctt
     5041 gtaaaaaaaa atacagaaag aagcataagc ttctttctgc atttttacgg tacttgatta
     5101 ctcaagcaga caagcccttc tccacaaaag tcttgcccga cacccctgca tccggcaggt
     5161 gataaagata gaaatcaatt atcctgtgcc tccaaaatca attaggagct ctgtaaacca
     5221 gagtccttct cgacccgtcg cataaattta ctttaacaca gttcctatgt ttatgcgaga
     5281 gcttttttca ttttataatc gaccatttga aatccttgac cgatatctga aataagttct
     5341 tctgtaatct caaatccttc atgcttataa acctcgattg cttgatgatt cccctgattc
     5401 actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa
     5461 actgaagaag tcaatccttg accacgtact ttatttagca aatataattt acttaaataa
     5521 atttttccat ttatctcttc ataagcagtg taaccgacag tcttttcttc gctttttaat
     5581 aaaaagtagg acacaccttc tgtcagttct ttttgaatct gtttttttga ctgataagtt
     5641 ttcagcatgt aatccacttg tttttgacca atgatcggca caaaagcttc cggccatact
     5701 tcttgtatga ctgccatcag ctcatccagt tcattttctg ttacttttat tttttccata
     5761 cacccacgtc cttttttaca ttataaatga ccttgcgctt gatggaataa aaagagcatg
     5821 atcatgctat acacacccac cgaccatggt ttcaagaaaa tcacaatctg cataaattcc
     5881 ctaaaagata atttcgtcaa accagctact agacagatca agtcgtctgg cgcaaaggga
     5941 agcagcaagg taacaactag tactttttta atatctttcg tttttcttgt cagcaattca
     6001 tcaaatttct gatacttttt tggcgataaa ataagctgga caaaggctac gccatagtaa
     6061 cggacaagca aaaatccgat tgcttcccca atcactaggc caatacaact atataaaagc
     6121 ccaaatccat tgccaaacat cagcatgccc gctactgagg agatccctcc cggcaaaata
     6181 ggaatgatca cttggatcac ttgcaacaga acaaatatca tcactgcata cttgccaaat
     6241 tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg ataagcataa
     6301 agaatcaaca caaagaaaaa aaacagacca ataatgggca tgatctgaag acatttttgt
     6361 aaaactgatc cttttttaga catctatatt ccccacatct tttttcattt attaaaactg
     6421 cctccttatg atgtgcaccc caaaagttag actagaaatc taattaaaag gggtgctttt
     6481 tttgcggaaa tatacatttg tttttaagaa aaaagtagtt tcagactatt taaataacga
     6541 aggcggctac aaatatcttg cacataaata tcaaataaat cgtacactgg ttagacattg
     6601 ggtaaggatt tataactatc atggttggga aggcttggtt ggaggtggca aaagctacac
     6661 tacaaaattt aaacttgatg ttatagaata tatggaaaca aatggtcttt ctatccaaga
     6721 aactgctaaa aaatttaata tcggttcaaa tagaactcta agtaaatgga tagagcaata
     6781 tgaagaaggc ggtgcttctt cacttgagag ccaaaaaagg ggcagaaaaa ttagtatgaa
     6841 ttccaagcta aacattccta aaaaacttaa agatgagtct cttgaagaag aagttattcg
     6901 tttaagagca gagaacgcat atttaaaaaa gttagaaacc ttgattcaag aacaagattt
     6961 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
     7021 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
     7081 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
     7141 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
     7201 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
     7261 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
     7321 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
     7381 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
     7441 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
     7501 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
     7561 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
     7621 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
     7681 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
     7741 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
     7801 cggaaacagt ccattaaata gttcaacttt tggggttcag
//
LOCUS       DAYLZO010000121         7760 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.123, whole genome shotgun sequence.
ACCESSION   DAYLZO010000121 DAYLZO010000000
VERSION     DAYLZO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.123"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACGVJG_002522"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACGVJG_002522"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFJ6558435.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACGVJG_002523"
     CDS             complement(2032..3285)
                     /locus_tag="ACGVJG_002523"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFJ6558436.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACGVJG_002524"
     CDS             complement(3291..4214)
                     /locus_tag="ACGVJG_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFJ6558437.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACGVJG_002525"
     CDS             complement(4435..6927)
                     /locus_tag="ACGVJG_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFJ6558438.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACGVJG_002526"
     CDS             complement(6934..7401)
                     /locus_tag="ACGVJG_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFJ6558439.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="ACGVJG_002527"
     tRNA            complement(7537..7610)
                     /locus_tag="ACGVJG_002527"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="ACGVJG_002528"
     tRNA            complement(7668..7741)
                     /locus_tag="ACGVJG_002528"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DAYLZO010000122         7635 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.124, whole genome shotgun sequence.
ACCESSION   DAYLZO010000122 DAYLZO010000000
VERSION     DAYLZO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.124"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..147)
                     /locus_tag="ACGVJG_002529"
     CDS             complement(<1..147)
                     /locus_tag="ACGVJG_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFJ6558440.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVK"
     gene            328..639
                     /locus_tag="ACGVJG_002530"
     CDS             328..639
                     /locus_tag="ACGVJG_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558441.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(754..3090)
                     /locus_tag="ACGVJG_002531"
     CDS             complement(754..3090)
                     /locus_tag="ACGVJG_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFJ6558442.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            3232..3900
                     /locus_tag="ACGVJG_002532"
     CDS             3232..3900
                     /locus_tag="ACGVJG_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFJ6558443.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            3903..4946
                     /locus_tag="ACGVJG_002533"
     CDS             3903..4946
                     /locus_tag="ACGVJG_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6558444.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(5183..6064)
                     /locus_tag="ACGVJG_002534"
     CDS             complement(5183..6064)
                     /locus_tag="ACGVJG_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFJ6558445.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            6223..6951
                     /locus_tag="ACGVJG_002535"
     CDS             6223..6951
                     /locus_tag="ACGVJG_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFJ6558446.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            7107..7424
                     /locus_tag="ACGVJG_002536"
     CDS             7107..7424
                     /locus_tag="ACGVJG_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFJ6558447.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            7417..>7635
                     /locus_tag="ACGVJG_002537"
     CDS             7417..>7635
                     /locus_tag="ACGVJG_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFJ6558448.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKN"
BASE COUNT         2363 a         1297 c         1541 g         2434 t
ORIGIN      
        1 ttttactgta ccgccagcac cgatataacg gtaaacacca gataattctg cttcaggatc
       61 gatttcaata tctgtttcgt gatattgacc tggaagtgtt tttagttctt cgatgacttt
      121 tcttaaatca tacggttgat ctgtcatgag agattcctcc ttagttgtct ttacctatct
      181 atagtaaaag gtgaaaaaga ggaaaacaat tcaacttgtt cggtaaagta ttctacaatg
      241 gaatggatgc gtttttttga taatagaata aactatgata tacttatcac taaaagaaag
      301 cgcatactta gtgttggagg aaggaaagtg aaaaaaatgg aggagtcatt tcctaaagca
      361 gtaaaagtgg aaaatatagc gaatattttg aaagttacat ttgaaaacgg agaagtaaaa
      421 tacgtgaaat ctcattggac agaagagata accgatgcct tgcaatttgg aaaaaaagga
      481 agaggaaaaa gaaaaaatct tctcgcactt tctcgtaata tgtggattgg gactgaagta
      541 acgattgaag cagatggaac agtattcata aatggaaaag acaggtatac gccagaagag
      601 ttgtggtaca aaggaaagaa aagtatacca gaattataaa aaataattaa aaaaaacagc
      661 gcgaaacaag gaacatatta cttcctcgtt tcttgctgtt ttctactatt tcagcgctct
      721 tatgactcaa atctaaatgt ggttgtcaca accttattct gcacgcaaag cgatagcggc
      781 gtcttttttc gctgccattc gtgcaggaag atgaccgcca agcatcgtca atatagtaga
      841 gacgatcact agaataatcg catggacagg atttagttga gcaacatttt ttagatccgt
      901 catgttgtat aagatgctat tgatagggaa cgtagctagc catgcaataa acacacctaa
      961 tatcccagaa gaaataccta aaatacacgt ttctgcgtca aatacgcgag tgatgtcttt
     1021 tttacgtgca cctaaagctt ttagtacacc gatttctttt gtccgttcaa tcacagatgt
     1081 ataagtaatg atactgatca tgatcatact tgttactaaa gagatccctg caaaagcaat
     1141 aaggacataa gtgatagcat ccatcaatcc gccagtcagt tcagtcatcg ttccagctaa
     1201 atcagtataa atgattttat cttcatctga tttgccttta ttgtaatcat ctagataatc
     1261 caagatcttt tctttgtctt caaaattgtt cggataaatc atgatacttg aaggcaagct
     1321 gtctccacct aaataactaa gcaatgtttg tttggcattt tcgtcaactt tttcagtagt
     1381 taagacattt acaccactgt ctctttgagc tttgacgatt tctgattctt tgttttcgtt
     1441 gacgatttga gtcgttaatt gatcgctata agcgattcct ggagataaga gattcattgt
     1501 agaagaagat ttcacgcgta ggatccccga gatcttcaat tcatcagaag cattctgata
     1561 catcgcatca taatctgtat tagggataaa attcccagtt ggaagttttg tatagtaagt
     1621 attgttgttt actaatttga atgttgtacc aacgatatca tcaaaatcaa gtttttcgtc
     1681 ttctttcaca tcaaatccaa gattcttcaa tgcgttgata ttagtattat tattgccgtc
     1741 tacgatcagc acgacatctg ttgctgaggc tggataactt ccggcaagta aagaataatt
     1801 gtcttttaaa aagttttctt tgctagtgtc aagctgtgta gggaaacttg acactcccac
     1861 acctgtcatt gctgacatcg tactagataa agaaagagat tccgtatcag gattttgatt
     1921 cgagaagctg acaggctgta ctttgccatt tacatcacgt aaaagattga ttcctgtagt
     1981 acgggtaaat ccgatattat tacttaagtt aggatcgatg tctgttacgt aatcgacgta
     2041 tgtttgatca atcttattcg tgtgctgtgc tttatcttct tcgctgactt ttgcagtaat
     2101 cgttttgctg tctggataat ccgctttgct tgcacccaat ccggcatcgt cacgagtctg
     2161 gttagtcgtt accttagaaa tagtaatcgg aaatttcgcc atcgtttcag cttgcgtgtt
     2221 atcgatttgt ttttggaatc cagaagaaag ggacagaacg atcgcgattc cgatgatccc
     2281 aatactggaa gcaaaggagg tcaagaaagt acgtcctttt tttgttcgga tattgttgaa
     2341 ggaaagcttt aacgctgtcc aaaaactcat ctttgttctg cgtaagttaa attgatcatc
     2401 ttttggtcgt tcgatgtgag gatgggaatc tgttaagatc ttaccatcag aaaattcaat
     2461 gattcgatct gcgtattgat gggccagttc aggattatgt gtgaccatga tcactaattt
     2521 ttctttcgat aactcttgga tcaattccat gatctggata cttgtttctg tatctaacgc
     2581 tccggttggt tcatcgcaca aaaggatatc tgggtcattg gctagtgctc gagcgatggc
     2641 cactcgctgc atttgtccac cagataactg atttggcttt ttatgcatat gatttgtcaa
     2701 gccgactcgg tgcaatgcat cttctgcccg ttttcgtttt tcgtctttag aaacaccact
     2761 taatgtcatc ccaagttcaa cgttttcaat aatgcctaga tgaccaatca agttgtaact
     2821 ttgaaaaatg aaaccgatag agttgtttcg gtaggcatcc cagtcactgt ccttaaagtc
     2881 tttagtagac tttccgttga ttaccatatc accggaatca taattatcta gtccaccaat
     2941 cacattcagc atagtcgttt taccagaacc actaggacct aaaatcgcta cgaattcttt
     3001 ttgtctgaaa gcgacagaca caccatccaa tgctttggtg gtggtttcgc cgactttata
     3061 atattttttt atatctttta attgaagcat aatcaccctt ctcccgtatt gaaatattcg
     3121 gtatactaat aagtatatca gaacaatgat cgtagtatac gaaataaatg ttaactgaag
     3181 atgaactaca agaaaaaatc tagaaatttt tcaaaaaaca gagggagatc aatgaatcgt
     3241 attttagtag tagaagatga tccgcaatta cagttgcttt accgttctgt gttggaacga
     3301 gtgggctttt cagtcatcac cgcattgaat ggagaggatg cgctgaaaaa actagaagcg
     3361 acacgtatag aattaatcat tacagatatc atgatgccga aaatggatgg ctatgacttg
     3421 ctagactcat tacgtagcac cagatttgac acacctgttc taatgattac ggcaaaggcc
     3481 gattttgagg ataagaaacg cgggttcaaa ttaggagcag atgattatat gacaaaaccc
     3541 atcgatgtca atgagatgat cctacgtgtg gaagcattgc ttcgacgttc aaagatcaat
     3601 tacagcagta gcttgataat cggacaaaca aaattagagc tagatgctta tacagtgtta
     3661 caagatcagc attcgttgat tctgcctcaa aaagaatttc tgcttctgta taaattactg
     3721 tcttatccca acaagatctt cactcgccaa cagttgatgg atgatatttg gggattagat
     3781 acgaatacag aagaacgaac gatcgatgta catatcaaac gtttaaggac ccgttttgaa
     3841 aagacggaag attttcaaat catcacagta cggggactag gctataaggc ggttatttaa
     3901 agatgaaaaa aaagctattt tcacaattat ggatctattt tgctagtatt gtcttcattt
     3961 cgatcttagt gacactgctg tgttttctcg gttttatttt cttattgtct cgacattcta
     4021 tctcgcctgc tgaatcggct ggaaaaccaa cgttataccc gttattcgtc tttgcggggt
     4081 tcagtatggt cgtgggtaca ggaatatcga tttttgttgg gcggcgtatc ctgcatccaa
     4141 tcagtgcgct agggacaaat atgagtctgg tagcaacggg agatttttcg attcgaatgg
     4201 atgaacagca gaaagtagca gaagtacagc aactgtataa agatttcaac gttatggtcc
     4261 aagaactcaa tagtatcgag acactgagga atgactttgt gtccagtgta tcccatgaat
     4321 tcaaaacacc actagcaaca atccaaggat atgttcaatt gttacaagcc ccaaatctct
     4381 ctgacgaaga acgccaaata tttttatatc ggattatcga aagtatcacg cagctttcac
     4441 aattaacaga gaacatctta aaattgaaca aactggaaaa ccagcgtatc caactggaaa
     4501 agaaagagta tcgattagat gaacaaatca gagaagtaat cgtttttttt cagcctaaat
     4561 gggagaaaga acagctagaa ttggatatcg aacttgctgc agtaaattac acaggcaatg
     4621 aagagttttt gtaccaagtg tggctgaata tcatggataa cgccataaag tacaatcaaa
     4681 tcaatggaca gattcatatc aaactatttg agacagccac agagattgta ctggaagtga
     4741 ccgattcagg tgtaggaatg aatgaagaga ccagagatcg aatgtttgaa aaattttatc
     4801 aaggagatac aagtcgtcaa atttctggga atgggctagg actttcactg gttaaaaaaa
     4861 ttctagaact gcatgatgga cgtattgatt atagcagtat cgaaggagta ggaacgacag
     4921 caatgatacg gttaagtaaa caataggaaa aagaataaaa cccctaaaaa ttctattgaa
     4981 ggtttttagg ggtttttagt cgtttttttg tgattaggga aatgttctta cggttctttt
     5041 ggcataaaaa tcgctgcttt cttattgaac tacgagaaat gttagaggtg tcattgaaac
     5101 aaggacatga tgcaagaaag agagcgaaag tcatgcgtcg ctctcttttt ttgggtggat
     5161 agatggacag aaaatgtagg gcttagtttt cttcttgatt taaatactcc caaaatgtat
     5221 ctgaagccaa agaatgctca ccgactttac gagtaaatgc aagataactc tctgttgttg
     5281 gagaaatagg gagaatcaca gtattttctt ttaatagatt cttgactgtt ttttccataa
     5341 caatagcaat ccccatgtca tttgcaatca tattgatgat caagtcgatt cttgctcctt
     5401 tataagagat ttttggttca aatccagctt cgcgacacag atctagtaca ggttgataca
     5461 agttcgtagt ggggccaagg agcaaaaagt tctccttttt cagttcgctt agatccaatt
     5521 tctttttact agctaaagga tgatttttag gtagaactgc tacaaaatcg tctgtttcag
     5581 taggcaacca ctcaatcgaa cttggtgggg cctggaaact tctagcaaag tggatcgtgt
     5641 cactatgttc attggcgtca ttgtaagtta attcgacact ttccacttct ttcaactgga
     5701 ctgtaaattc aggatggcgt ttcaaaaact cggtgatttt ggaaaaacct ttataattga
     5761 tcattgtcgg tatcgttaag atatttaatg aaagattgtt gtctttcgta taggtttcaa
     5821 gcacttcttg catagcatgg tattgttcta cgacattttt tacatatggg agcaataagc
     5881 ttccagcttc ggtcaattcg atttttctat gggcgcgttc gaataatttg acgtctaatt
     5941 ctgtttctaa cgaaagaatc tgtttagaaa tatttccttg ggtggtgaat tgtcgatcag
     6001 cagttttggt gaaattcatt gtttcggcta aatcaagaaa aacttcgaat ttatggatat
     6061 ccatcatatt cctcctactt attccaataa agaatacttt aacacaaaaa atgaattggt
     6121 gtaatcttct ctgtctgtta caatctttct tgcagcgaaa gcaataatta cagaaatttt
     6181 tctgattttt ttcgcttcgt agaaaaagag aggagttttt tgatgccaac tggagttttg
     6241 atcaatgtag gttccgtttt gtttggcgga ctaattggcg gattagtagg aaataaatta
     6301 agtgaacatt ttaaagcaca gctgacgatg gtttttggtg tgtgttcgat ggggatggga
     6361 atctattcca ttgcgccgat gaaaaatatg ccggctgtta tttttgcttt agtgatagga
     6421 acagcgattg gtttgattgt tcatttaggt aatgggataa ataagggggc tgcgctgatg
     6481 caagtaccta tttctaaaat cttccctagt gaaaagcttg gtatgaccca cgatgaattt
     6541 atcagcacat tagtgacggt cattgttcta ttttgtgcaa gtggtacggg gatctatgga
     6601 agtttagatt ctgggatgac aggagacagt acgattttga tttctaaatc tgtccttgat
     6661 tttttcacag cagctatttt tgcgtgtaat ttagggtatg tcgtttctgt gatagcaatt
     6721 ccccaattca ttatttttta tattttgttc ttattggcaa aatttattta tccattgact
     6781 acaccagaca tgatcttaga ttttaaagct tgcggcggat tcttgatggt cgctaccggt
     6841 tttagaatga tcaacgtgaa aatgttccca gtagctgaca tgatacctgc aatgatcgtt
     6901 atcatgccac tgagctggat gtggacaaat tggattatgc cactgttata ataaaaatca
     6961 aagagaagcc attttttgct gactaaaaag gatcaaatct tttagtcagt ttttttgtat
     7021 ttgacaaact gattttgaaa ctatattata tgttgtataa tatagattga aataaaatat
     7081 tacacttgag aatagaaggt gacgtaatgc acatccagat tccaactgtt cttcttgatg
     7141 gtactgtgct agcagcatta agcaaggaag atatgtatgg gtatgcctta acaaaaatcg
     7201 tacaagaaag tctctctgtc agtgaatcta cactatatcc ggttttgaga agattgaaaa
     7261 aagaaggata tcttgagacc tatgatgaac cttttgaagg cagaaaccgt cgatattact
     7321 cgttgacacc aatgggaagt cgtcatttag aagaaattat tgatgaatgg attgtttttc
     7381 gagattcttt agaccggtta ttggaggaga gcacaaatga atgaatattt gaatgaagtt
     7441 gtggatcatc ttcaagaaat tcctgaagac gatcgttttg atttgatcca gtattatgag
     7501 gaatattttt tagattctgg aaaaacatta gatcagatca ttgatgaata tggtacacca
     7561 aagcagtttg ccttaaaatt aaaaatcagt tacttttcag aagaagaaca agcagaaaac
     7621 gagttagtga aaaat
//
LOCUS       DAYLZO010000123         7599 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.125, whole genome shotgun sequence.
ACCESSION   DAYLZO010000123 DAYLZO010000000
VERSION     DAYLZO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.125"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(136..426)
                     /locus_tag="ACGVJG_002538"
     CDS             complement(136..426)
                     /locus_tag="ACGVJG_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558449.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="ACGVJG_002539"
     CDS             complement(544..804)
                     /locus_tag="ACGVJG_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558450.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="ACGVJG_002540"
     CDS             complement(941..1354)
                     /locus_tag="ACGVJG_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFJ6558451.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="ACGVJG_002541"
     CDS             complement(1810..2295)
                     /locus_tag="ACGVJG_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558452.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="ACGVJG_002542"
     CDS             complement(2434..2649)
                     /locus_tag="ACGVJG_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFJ6558453.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="ACGVJG_002543"
     CDS             2873..3673
                     /locus_tag="ACGVJG_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFJ6558454.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="ACGVJG_002544"
     CDS             3692..5560
                     /locus_tag="ACGVJG_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFJ6558455.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="ACGVJG_002545"
     CDS             5553..6044
                     /locus_tag="ACGVJG_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFJ6558456.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /locus_tag="ACGVJG_002546"
     CDS             6032..6586
                     /locus_tag="ACGVJG_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFJ6558457.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="ACGVJG_002547"
     CDS             6604..7599
                     /locus_tag="ACGVJG_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFJ6558458.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DAYLZO010000124         7436 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.126, whole genome shotgun sequence.
ACCESSION   DAYLZO010000124 DAYLZO010000000
VERSION     DAYLZO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.126"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1803
                     /gene="fss3"
                     /locus_tag="ACGVJG_002548"
     CDS             <1..1803
                     /gene="fss3"
                     /locus_tag="ACGVJG_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFJ6558459.1"
                     /translation="QEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTLFTAKDGKAVKW
                     SEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAPEGYSLDETKYP
                     VSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGFNDLTFKVSPLE
                     GTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGFQKITPLEIRST
                     FKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNRLMLYDLPEEED
                     SLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDVDATKAAQEKDE
                     KAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYENKEAFEAGDKP
                     VAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFDDVSITHDVLDG
                     SKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDTGKYPEGTKFTF
                     AEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVPSGSQESSPTVK
                     TFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            1916..2230
                     /locus_tag="ACGVJG_002549"
     CDS             1916..2230
                     /locus_tag="ACGVJG_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFJ6558460.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            2243..2617
                     /locus_tag="ACGVJG_002550"
     CDS             2243..2617
                     /locus_tag="ACGVJG_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFJ6558461.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            2627..2962
                     /locus_tag="ACGVJG_002551"
     CDS             2627..2962
                     /locus_tag="ACGVJG_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFJ6558462.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            3045..4385
                     /locus_tag="ACGVJG_002552"
     CDS             3045..4385
                     /locus_tag="ACGVJG_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFJ6558463.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            4463..5137
                     /locus_tag="ACGVJG_002553"
     CDS             4463..5137
                     /locus_tag="ACGVJG_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFJ6558464.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            5130..5294
                     /locus_tag="ACGVJG_002554"
     CDS             5130..5294
                     /locus_tag="ACGVJG_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558465.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            5370..6512
                     /gene="dcm"
                     /locus_tag="ACGVJG_002555"
                     /pseudo
     CDS             5370..6512
                     /gene="dcm"
                     /locus_tag="ACGVJG_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            6502..6792
                     /locus_tag="ACGVJG_002556"
     CDS             6502..6792
                     /locus_tag="ACGVJG_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558466.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            7051..>7436
                     /locus_tag="ACGVJG_002557"
     CDS             7051..>7436
                     /locus_tag="ACGVJG_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558467.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGV"
BASE COUNT         2447 a         1240 c         1691 g         2058 t
ORIGIN      
        1 caagaagtca ctggcgaaac cactttgacg aaagaagaca aagatactgg ggataaagca
       61 caaggtaagg cagtctttga gggtgctgaa tacaccttgt ttactgctaa ggatggaaaa
      121 gctgttaaat ggagtgaggc ttttaaacca gagttagcga agggaacgaa aggttctgat
      181 gaaacggtca ctttgacttt agacgaaaag aaccaagttg ccgttaaaca tttagctatc
      241 aacgagtatt attgccaaga aacaaaagcg cccgaaggat atagccttga tgaaacaaag
      301 tatcctgtat ccatcaaaaa ggtggatgat agcgaaaaaa atgccgtgat tacccgaaat
      361 gttacggcaa aagaacaagt gattcgcttt ggctttgatt tctttaaatt tgctggatca
      421 gctgcgggca ctaccgaaac tggcttcaat gatctgacct ttaaagtgtc gccattggaa
      481 gggaccaatg aaatcacagg tgccgaagat gaagcgacca cagcttataa cgagcaatta
      541 gggtttgatg gttatggcaa gtttgaaaat cttccttatg gggattattt acttgaagaa
      601 gtagaagccc cagaaggttt tcaaaagatt acgccattag aaatccgttc tacttttaag
      661 gaaaacaaag aagactttgt gaagagtgag tatgtcttta ccattaccga acaagaccaa
      721 aaacaaccaa ttaaaacggt gaccgttcct tacgagaagc tgacgaacaa agcattttct
      781 gttagtttga accgtttaat gctctatgat ttgcctgagg aagaagatag tttaacttct
      841 cttgcgactt ggaaagacgg aaacaaagaa ttaacttctc tggattctac cgagctagtt
      901 gataaattga gttacaatct acatgaaatc aaagaagact ggtatgtggt agcgcaagcc
      961 attgatgtgg atgcgacaaa agctgcccaa gaaaaagatg aaaaagccaa accagtggtg
     1021 attgcagaaa caagcgcaac gttggcgaac aaagagaaaa caggaacttg gaaaattcag
     1081 cataaattaa ccgccgaaca agttttaaat aaaactattg tcttattcaa ctatgtgtat
     1141 gaaaataagg aagcttttga agcaggcgat aagccagtag cgaaggatgt tagcttgaat
     1201 aaccaagccc aaactgtcag ttgtacagtg gaacaccatg tttctatcca aacaaaggcc
     1261 cacctagaaa acggttctca gacctttact catggtgatg tggtggatat gtttgatgat
     1321 gtgtcgatta ctcatgatgt actggatggc tcaaaagaag ctttcgaaac aattctgtat
     1381 gctttattac cggatggtac aaacaaagaa atttggaaat ctggcaagat tgattatgaa
     1441 gtaaatgata aagaattcac caaaactgtg cttgcgaaaa aagtagatac cggaaaatat
     1501 ccagaaggaa ccaagtttac ttttgcggaa atcaattacg ataaagatgg gaatatcaat
     1561 ggaaaacaca atgaagattt gaaagaaaaa tctcaaacct taacaccaaa agaagttcca
     1621 accatactaa gtacgccgaa acaaccggaa acaccaactg ttccaagtgg ctctcaagaa
     1681 tctagtccca cagtgaagac cttccctcaa accggggaga aaaattccaa tgttttactg
     1741 ttcattggtt ttactttgat ttttgcgacg gcgggctatt atttctggaa tcgccggaac
     1801 taaggtgatg agatgataca agtaaatcct aagaaccaac gtgcgcgacc gcctcctaaa
     1861 aaagtagtcg cgctattttt gtatccaaaa ctaacaatga aaagaggaaa agaagatgga
     1921 attgaaattt gtcgtgccag atatggctga aacctttgga aagttaagtt atgccggtga
     1981 aggagaaatt ataacagaag gatatggacg gaatactacg gtgattggtc gcagttatca
     2041 tttgtattcc agtaagcaac gggccgatga tattgaggtg gtagtagctg cggaagccgg
     2101 tgaaaaggat tttgatcaag accaaccgct aaaagccgtg aatccccatt tggttgccaa
     2161 aggctatgag attgagaatc gtgggtttac tgattacgtg ttgtatgttg atgatttagt
     2221 gaaagcatag gaggaaaaga agatgagatt aacagaaggt attgtcgtag attcaggttt
     2281 aacatttggg aagttgcgtt tttccgcatt acgccgagaa gtacggaagc aaaatgagga
     2341 tggaacaatt ggtaatgagg taaaagaacg aacctataat ttaaaatcct ctgcacaagg
     2401 gcgaatgatt caagttagta tcccagctaa tgtaccctta cgcgagtttg cttatgatgc
     2461 agaggtagag ttggtcaatc caatcgtgga taccgttgcc aattatgttt ttcgagaagg
     2521 aactaccgtt aattggttca ttaaggcgga tgatttagtc ttgaagcggc agccgaatca
     2581 aggaaatcct acgaaccaaa atgaaagaaa gaaataggtg aagattatgg aaattaatgt
     2641 tgaacatatt ttagattgtc tcgatcaata tggaaaaggc gagctgactg aggaacaact
     2701 cgttaaagca ttaacctctg atgaaaaaat gtttctgatt atacatcaag gtttacttgg
     2761 agaaaatagc gacagtgaag aagactctga tgtactagac tggttaggag aagaagagtc
     2821 tttctttata gttgtcgaag taaatgaaaa cttatgtcgg caggcagaat ccattttgga
     2881 agaaattggt gtggagatgc cggatgctat tgaaggcttt ttaaatcaat tggttgaaac
     2941 gaaacaactc ccctcctcgt agcagtaaat gactagcata actttttgaa attgcctaat
     3001 gaaatcctat tgggcttttt cgattggagg tggaaaccat caaaatgtac aaaggacatc
     3061 gcatacgcgc aggagatcag cacttggttt atcactttgt tcttggctgg ctactcgctc
     3121 tgttcatcgg ttggatgagc gtcttttatt ttcaagagtt tagacaattt gatatttcta
     3181 agctatcact ttctactatt gaaatcgttt ggtctataaa agatttagta tgtctgcttg
     3241 gaagtctcgc tttttcagga gctatgatat tgctttatat tcactttttt cttgatcatt
     3301 ggcgaagctt gtggcatcga caaaagctag ctcggatgat tctagagaac cattggtatg
     3361 aagtgaaaca aactcaaagt gaaggctttt tcaaagattt gaatagtagc cgaaccagag
     3421 agactatcag ttacttcccc aaaatctgtt atcgaatgaa agatggatta ctgtctattc
     3481 gcgttcaaat ttcacttggg aaataccaag atcagctttt aaaattggaa aagaagttag
     3541 aaagcggact gtattgtgag ttagtagaaa aggaactcaa agactcctat gtggagtata
     3601 ctttactgta tgatatgatc gccaatcgaa ttgggataga cgaagtagtg gcagaaaatg
     3661 ggactctgcg attgatgaaa aatcaagtat gggcttatga ttctttaccc catatgttaa
     3721 tcgctggtgg cacgggtgga gggaagacct atttcctcct caccattatc gaagcactat
     3781 tgaagtcaga tgcagaattg tttatcctcg atcccaaaaa tgcagattta gctgatttag
     3841 gtacggtgat gcctcatgtt tattcacaaa aggaagaaat ttctgcttgt gtggaagatt
     3901 tctatgaacg tatgatggct cgtagcaagg caatgaaaga aatgtctaac tacaaaacag
     3961 gagagaatta tgcctatctt ggacttccac ccaacttttt aatctttgat gaatacgtgg
     4021 cttatatgga gatgttaacg acgaaagaaa gtgcggtgat tttgaataag ctcaaacaaa
     4081 tcgtgatgtt gggtcgtcaa tctggtttct ttctgatttt ggcttgccaa aggccggatg
     4141 cgaaatattt aggagacgga atccgcgatc aattcaactt tcgcgtggct ttgggtcgta
     4201 tgagtgaact cggatattcc atgatgtttg gggaggtcga aaaaaacttc tttatgaagc
     4261 acatcaaagg tcgaggttat gtggatacag gtggcagtgt gattagtgag ttttatacgc
     4321 cacttgtacc aaaagggtat gatttcttag attcaattag taaattagaa aagatgaatg
     4381 attgagtgat cttcaatact gactttatac ttcaagtaac gtataatact tttatatagt
     4441 aggaagaaag gggataatta atatggaaag taatgattct tatttagagt atgcaaaagc
     4501 aaataaggat aagtttattt cttatattat caaagggaaa tcccccgaag ttggacgaga
     4561 cgcagttttt atggcaggga gtcctggggc tggaaaatca gaggttgcca ttggattagc
     4621 tgagaattat gataaccatg tgattattga tgcggattac tttcgcaccc agtttcctga
     4681 ttataatgga aaaaactcta gtctctttca aaaagcatct tcttggttgg tagagcaggc
     4741 atttagatat gtcttagatc atggctattc ttttattctg gatgcaactt ttgcaatatt
     4801 aagtgctgaa aaaaatatct cgcgggcgtt gaaaaaacat tttcgagtga cgattttcta
     4861 tgtttatcaa gaccctaaaa tcgcttggaa atttacacaa gaacgtgaaa ttgcagaggg
     4921 acgtcatgtg ccgaaaaagg catttattaa cgcttttttc aaatcaagag agaacattgc
     4981 aaaagtaaag agtaaacatc cagatgtatt gcttcatatc ataataaaag attatacgaa
     5041 taatatttcc gaagttcatt atgctacgga taatctagaa ttatttttac cactagggta
     5101 tagtaggaaa gatttggagg ttcaattaca tgactgagaa agaacaagta acgaaaattg
     5161 taaagaaata caataagagc attgcggatt tatcggaaaa tgctactgca aaagagttca
     5221 aaacggtgat taaatatgta gccgatcagg ccaatgaaaa acaacggaaa ttagttggat
     5281 taaataaaaa atagtaggac aaattaataa tgtatgagcc aagagcaaaa tgttcttggc
     5341 ttttttcttt gcaatgaatg aggtaatgta tgagattttt agatttattc gccggtattg
     5401 gtggctttcg tttaggtatg gaacaggcga gccatcattg tatcggtttt tgtgaaattg
     5461 ataaatttgc tagacgtagt tataaagcaa ttcatgatac aagtaaggag gtggaaatgc
     5521 atgacatcac aagtgtatca gatgagttta ttcaatccct cggaccagtg gacatccttt
     5581 gcggcggatt tccgtgccaa gctttttcaa ttgcgggaaa gcggcaaaga ttttccgata
     5641 ctcgaggtac tttattcttt gaaatcgctc ggttcaccac tcttctccga cctaagcttt
     5701 tattccttga gaacgtccga ggattgctca atcacgaagg aggggctacg ttcgagacga
     5761 tcctccgaac gctggatgga ttggggtatg acgtggagtg gtaggtgctt aactcaaagg
     5821 cctacattcc ccaaagccgg gagcggatat tccttatcgg acattctcga gacgcatgta
     5881 ccgaacaagt atttcctatc attggaccgt cttcaacatc tgatcaaaac atcagaaatt
     5941 tggtaaatat caatccttcc aatcgaggaa tgggtggaca agtctatggt tcagatggcg
     6001 tagcgccaac cttaacaggt gatgaaggaa tcaaaatcgc actgccagca agggatggaa
     6061 tctctgtagc aggactatta ccgggaaagt ttgaacaagg taatcgagtt tatgaagtta
     6121 ctggaaccgc ccccaccttg tcaacgaaac aaggtggtac aaaaatcatg attcgaaaaa
     6181 atgaaacaga atatcaagag gtgaagccag aagacagtgt gaatctggct tttcctaatt
     6241 ccacaagtcg ccgaggtcgt ctgggaaaac agagtgttca taccttacta acaggagacc
     6301 aacaagcgat cgtcacggat cagtatcaaa ttcggaagct aacacctaga gagtgttggc
     6361 gacttcaggg ctttcccgat tgggcttttg atcgagattc tcaagtcaat tcagatagtc
     6421 agttgtataa gcaagccgga aactctgtaa cagttccggt catttttgat attgccagaa
     6481 gattaaaaga gttggatgta catggtttat gatgagttga ttggagaaat ttattgggtg
     6541 attggaaaga ttcagtcaga cccagaatta gaagaacaat tgcgacggtt aaaatttgat
     6601 attcgcaaga atggtgtcaa agtcgctggc gatccatatt taatgaatga ggagacggat
     6661 gctcgaattg aaataaatca agtaattgca gaatttgagc ggattgcgga ccaagcaaaa
     6721 gaaccagata ttcgtcaata cttgttagaa atgaaagcag agttggaagt aaatggtatt
     6781 acagatgagt aacctaaacc aacacaactg actgttaggc gctgaccgcg gaggattcgc
     6841 gaagcagaaa tcctccgtgg tgctgtgcct gttcatggac ggcgttagcc gaccatgatg
     6901 tctcaccccc gtatctaaca ggggggtaca aaaacacaaa aaataagtaa gcaaccatta
     6961 agaatgcttg ttgtatcaaa gatagtaagt aatttgtata ggtgttaact catttgaaaa
     7021 agttaacacc ttgaatttgg aggtgagaac ttggcacaac ggaatttaga ttatcggcta
     7081 ttaaaagacc gacgcaacga atacggcgtt tcacaaaata aattggctac ggcttgtgga
     7141 cttagtcggc cgtacttaaa tcagatagaa aatggtggcg taaccgcatc tataaaaacc
     7201 atgaggaaaa tttttgacca actggaaagc tttaatcccg atttgccttt gacgttactg
     7261 tttgactatg taaggattcg ctttcccaca acggatgcac gaaaaattat tcaagaaatt
     7321 ctccatttga aatttgagta tatgctccat gaagattatg ccttttactc ctatcaagaa
     7381 cagtatgtca tgggtgatat tgtggtgatg ttgtctcatg aagaagataa aggtgt
//
LOCUS       DAYLZO010000125         7410 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.127, whole genome shotgun sequence.
ACCESSION   DAYLZO010000125 DAYLZO010000000
VERSION     DAYLZO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.127"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            109..789
                     /locus_tag="ACGVJG_002558"
     CDS             109..789
                     /locus_tag="ACGVJG_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFJ6558468.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            879..1913
                     /locus_tag="ACGVJG_002559"
     CDS             879..1913
                     /locus_tag="ACGVJG_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFJ6558469.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1927..2262
                     /locus_tag="ACGVJG_002560"
     CDS             1927..2262
                     /locus_tag="ACGVJG_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558470.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2246..4726
                     /locus_tag="ACGVJG_002561"
     CDS             2246..4726
                     /locus_tag="ACGVJG_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558471.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            4782..6341
                     /locus_tag="ACGVJG_002562"
     CDS             4782..6341
                     /locus_tag="ACGVJG_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558472.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6338..>7410
                     /locus_tag="ACGVJG_002563"
     CDS             6338..>7410
                     /locus_tag="ACGVJG_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFJ6558473.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGE"
BASE COUNT         2443 a         1359 c         1489 g         2119 t
ORIGIN      
        1 ttttatacaa aaagggtcat aatttcaagt aagcggatga attcatgagg attttttgca
       61 tactgtctct actatactta ggtcactaag aaaaaggagt gatttattat gaaaaagaac
      121 atgattggga cattattact ttcaacactt cttctaggcg gcttggcaac accagctttt
      181 gctgagggtc aagcgacttc aaaaggagat attaccttta ctgaaccaac aaataccgtg
      241 gagcctctta accctactga tccttcaaaa ccagtagagc cagcagatcc ggaaaatcca
      301 gcaactggcc agacaggtcc tttaacactg gatgtagttc ccgaattgcc atttggtaca
      361 cacgagattg aaagcggaac aaaaacgtat caagtagacg cttcaaaaaa cgatacacct
      421 tatctccaag tatcagatcg tcgaggtgta ggtgcagatg gccaagcaca agggtggaac
      481 gtgacagttt cggtttcaga ttttgtaaac ggttcacaag ttttgcaagg cgctgaatta
      541 gattttggaa catccactgt taaaagtacg tcagataacg aatccactgg accaactagt
      601 caaactgtaa ctgggttgag taaagcaagt gcagcgacac caatatttac agcagcaaaa
      661 gatcaaggtc tgggaacatg gctttctgtt tatgatccag caaatatcac actgaaagtg
      721 ccgaaagcag cagctggaac attcacggct gatttgactt ggaacctcgt ggctggccca
      781 gtagcataga gtatgaaata aattcaacaa actgcggtga aaatactata ggtgaaggat
      841 tttttaccgc agtttttgtt gaaaggatgt ggtggacaat gaatcaaagt aaacgactgt
      901 ttgtttcgtt tttttctatt ttaagtctat ttttcatttt tccgtcaatc agccaagcaa
      961 aggattcagc aggagacttt ggaatcaaac cggtatttcc agagaaccaa atagataaag
     1021 caatcggtta ttttgattta ttagtggcac cggaacaaaa tcagacacta gaagtaatta
     1081 tcagcaattc ttcagatgaa gaacgaacat ttgaggtctc tgtcaatccg gcggtaacga
     1141 gtgatggtgg aacgatcgat tatagtcaaa aaaacccaac gttagatgag acattgccgt
     1201 ttgatgtccg tgacgttttg ctcattgcga aaaaggaaat caatgtatca gcacatgcag
     1261 aaacaactgt acctatagaa gtgaaaatcc cagcaaaaag ctttaaggga cgtgtattag
     1321 cgggtattca tgtttcacct aaagaagaag ctgaaacaga aaatgctaca gaaggagcac
     1381 aaatcaaaaa tcgtattgct tataatctcg cagtcgtttt acaagaaagt caagagacga
     1441 tagaaccgga tctgaaactt ttatcaggag atttggacga ggtcaacgca aagccaactg
     1501 ttcagcttcg tttccaaaat cctcagccta ggattatcag caatttgata tttacgagca
     1561 agatctttta tgaaaatcag ctttatattg aaaatacgtc taatgcattt ttagttgcac
     1621 caaattcgaa ttttcacttg aatcttgatt tagctggtga taaagcaaaa gccggcgatt
     1681 accgcgcaga aatcatagca aaatcaggcg acagtaatga atggcgattt acacaaaact
     1741 ttacgatcaa aaaagaaaaa gcacaaaaag taaatgaaaa ttccgttttt gcagtacaag
     1801 aacaatcttt cccatggctt tatgcaggat tggttgctgc agttctagtg ttatggttga
     1861 ttttgctgtt tttgtgggtc cacaagaaaa agaaaaaagg agaaacaaca tgatgaggtg
     1921 atttcgttga aaacatcgat cttattggga gggattattg caagtttgct gttcttcagc
     1981 tggcaagagc cagtccaagc agataccaaa agttcaggta cgattacatt ttatgatcta
     2041 agtccaattc cgcatccaag tggttcagtc aatggcgcat caaaacagca gacacagcta
     2101 ccgatttata aagaatctaa tcaacatggc gtattcccgg agacgaatga acaaaagatc
     2161 cgttttcttt atttgatcat tggtatagaa ttatggcttc ttgcgtggtt gttgttatgg
     2221 agaagaaagg aaaaggagga tgaacatgtt gctacaaaat aaattttcat tttttcgctc
     2281 cttctttttt acttcgcttt ttgttttaag tatatttatc ttttcacctt cggcagaagc
     2341 tgttggagca acgcctcctg ccatttctta tgatccatac acgtatgaaa atatcgtacc
     2401 tccaaatggt ggacgtagtg cagtcaatgg cgggaagctt gtccaactaa ctggggctaa
     2461 ccaattgcct aaattgactg ctacttattt gaacgatggt tcaaatgatc cttggaaagg
     2521 tggcacggtc cgttttcatt gggaaagcca tgtgaaacta acaaatgtac ttgatttggt
     2581 gaatatcact gcacaaggat ttgcatttgt ggcgatcatc aatttgccgg aacatgtatc
     2641 aagcagtgat gtattaaaag caattgactg gaacaacgcc tacttgtata ttgccggtgc
     2701 ccccatcaag gttactagcg gaagtttatt atcagcagga agccatacct tgcgttttgg
     2761 gatggggtcg tgggatacaa atactctatt gacagatatt attgctatta ttacgcgagg
     2821 cgaattcaac gtggacgata tcccgcttca atttgatgtt acagctaatg tcgcgaagat
     2881 gacagaaaac ggagataaat atgatacgag tccaaacaaa attttgacca agggtaaatt
     2941 acaaccttct atgtctaaaa aggccgtttt ttctgttgat ttttatgatt cgaataatat
     3001 tatccaagat cgtatgttca attcgatttt agctccagct gcaggtagat tttatcctaa
     3061 attaactggc ggaaacttga atccaggagc atatgcattt tctgcaagtg cagagataaa
     3121 tacgtggaat agttacatct caccaagtga ttcaacagga aaatataata catttgctga
     3181 taccacagaa tacgtggatg gaaataacca gaaccttcca ggctctacca atagtcggac
     3241 attgacgatg gggctgactg aaggacgttt tgatcagttg ccatttagtc gctttgatcg
     3301 cgtagtcaac tattttacag gaaaaaacgt tacacaagga gcaatactga ctcatactcc
     3361 agcaagcaca agtggattga atcagacagc caatatcgtg tacagtggga aagatgcgca
     3421 aggcggattg ctttctcctg ttgctttaaa ggtattggag cagtacaagc tagacggaac
     3481 ggttttacca acaaacttaa cagccgatag tcagtgggga aagctccttt cttctgcatc
     3541 gtaccaagtc acggccaatt ggtcagcaac tgaactaaaa aagggttcga tccattacag
     3601 gctttattcg aaaggaaccc aaaaattagc gacaggatgg acagatcaac ttttccaaac
     3661 gatccaatca aataacgggg gtgtaaatac agctacttct acactacctg cattggcttc
     3721 agggcaatat tattttgatt atcgcttggt ggacgatgta cttactaaca tgtatccgtc
     3781 ttttgcgtac aaatggcagt ctgaacaagc tggaatcacc actcttcctc aaataacagt
     3841 ggctaatttt ccatctgtga gtgcagatag tcagctaaaa aaccttagca gacaacctga
     3901 gacaggagat cccctgtctg ctctatcagg agatacgatt caagaaaatg tgacattcac
     3961 gttaacaaaa tctggggatg cgataaccga caaaaaaatc acgatctcac ttcctgaaaa
     4021 tactacttat atcaatggtt ccttaaaatt aaatggtaca actatcgcag ataccggcat
     4081 tcaacaagga gtttctgtcc cagcagatct attgagcaaa ataggagata ccattcatct
     4141 tacttacaac tatcaattaa atactgtaga tacttcttta caatccgtca gcatactgac
     4201 aaaggcagct gtactttcca gcaatattac attagcagat ggtgctaagc ttcctaaccc
     4261 agttgtccaa acatctgcta aaacaattct tgtccccaaa caagaattaa cattagttaa
     4321 tgtaccagat gattttacat ttggtaacga tctccccaaa ccgttgaaga caagttatta
     4381 tgaatcaaaa ggcgattttt cttttgatgt gcgggatact cggcttccaa gcacgtctcc
     4441 ttggcaactg acaggtacat tgacttcttt atttaagaat gatcaaggtc aagaattaag
     4501 cggaacaaaa ttatacttca atcattctgg atcaaagcaa ttgattcagc aaggtcaaaa
     4561 cacgttgatt tatgagagtg atggaacagc taaaggagag gttctcgttg actttcccga
     4621 taccgatggt ttactcttag aggtaaatag cagtacgaat gctcaacctg gcgcaacgta
     4681 tcaaggcatg gtcacatgga aactgacagc tgggccaacc agctaagtga ttagaatttg
     4741 tatggatctg tgataactgt cacagatcct ctttttcaag gatgagaagg aaacttctgc
     4801 tgttaaaatc agaaatgctt gaaggagtgg acttggaact tttcagtttg cttgaaaaaa
     4861 atgaacaacg gcaaatcaaa cttttccaac ttttattaga ggaaaaagga gaggtttccg
     4921 ttaataacgt acttacccgc ttatcagttg atcgggctac cttgaaagaa gatctgtttt
     4981 atttacaagc taacttggaa aatatcaaac aattggaaat tgttatccaa gaagggaata
     5041 ttttattaat cagacatgga aatatagcag cagtcgatgt ttattatcat tgttacttgc
     5101 gctattctac aaaatatcag atacttcttt atctgttgga aaatggctca tttgatcggc
     5161 agaaacttgt catggaactt aatatcagtt cggctacttt gtctcgcaga gtgaaggaat
     5221 taaatgattc tttaaatgag tttcatctga aaataaaaag cgggcggctg accggtccag
     5281 aaagtcaaat tcgttatttt tatttccaat tattttggtt tggacgtccc tatcttgaaa
     5341 acaaaaaaga atacagtgat ttatcaggaa acactttttt agatctgctg caaaaagagt
     5401 ttagttttgc tattacagaa gaaggacgat tgaaatttga aatatggctt tccattatga
     5461 agaagcgaat gcgctgtacg aaaaaatcca aaccagatta tagcaatctt cctgttggat
     5521 tgtttcaaac gacacaattt ttattggacc tccatacatt tttgacccgt tacttctttc
     5581 aatatgcctt tccttggaat caaaatgaaa cgttggtttt ttatcttttc atgatcagta
     5641 actttacatt agatacacat aactcgcatg tccaggagtt tttgataagc gaaaaataca
     5701 aagacgacgt agtagctcaa ttgaatcgaa cgtttaaaca atcgatagaa gaatatttct
     5761 ctttttatgt ttttccagaa gagatttcgc aaaagattgc gttgacagtg atgcaaaatc
     5821 acgctcattt tgcctatttt agaggatgga tgaatatttt tggccaagaa ggattaaaac
     5881 agcggttaac tgaagtagaa gataaacaat tcctggtttt atgtgaaaga ttggtcagac
     5941 aaacatttgc tattgttgat tttactgaag tagacgagga aaatgcccgc tttgaattat
     6001 ttgctcgcta tgcatctatt ttacggacgc tgatcaagca gttagaatta cgtctgcaaa
     6061 ttgcttatga tcttccttat gaaaggctaa tggcggatat cgtgaaagaa atcatccagg
     6121 aacaattgct gaatacgatc aaatatgatt tagtcgaata cgagaaagaa aagcagtatg
     6181 atatcatttt gactagccaa ttgaaagagt acccgtcaca gaagtccgct aaagtttttg
     6241 tattcactag caacgaatat aattatgatt tcccctattt gaaccaattt ttaaaagaat
     6301 gttatttaga aaaattgaat ttaaggatga ataaaacatg aaaatcgtga tcattggtgc
     6361 ttcccatgca ggcatcacag ctgccctaaa tctaagaaaa cttcaaccga aagcagaagt
     6421 tttactgatc gatgaagatc ataaagatgg actaggctat gtttcaaatg gaatcaacct
     6481 ttatttaaaa ggaaagatac gtagtttagc tgaagttgcc cataacatgc ggactcttca
     6541 agaaagtggg gcaaaactga tcaccgaatg gagagtaact gaacttgatc cagacaaaca
     6601 tcagctcatc ctcgcatcca aagaaggaaa aaacgagacc ataacctatg ataaattaat
     6661 cgtagcgaca ggttcctctc ctgtcacctt gtataaacaa attgaagctg aaaatgtcta
     6721 cacctataaa aatctagtgc aatctaagca agtacttgcc gctttaaaag aagcaaaaga
     6781 agtagtgatc ttcggtgctg gttacattgg attggaatta gcagatgctt tgagaaataa
     6841 aggatacatt attcatctag ttgattatat gccgaatgtt ttatcaagat atttcgataa
     6901 ggatatgatc aacagttttc aaaatcagct gcaaactaag caaataaatt tttatccaaa
     6961 tgaattcttg atcgattgga aaaaatcaga agagaaagta gtatccgtac aacttctgtc
     7021 tcaaacaata aaggcagata tggtcatttt ttctgcccaa actcgtccta atacgacatt
     7081 gttaaaagaa aaagtggctt tgtatgagga tgagactgtg atagtcaatg aatatctgca
     7141 gacaagtgat ccggacatct atgcgattgg agatatcgtc ccagtcagtt ttgacaaaaa
     7201 taaacggcat ctctttttac ctttagttac tcgagctgta catatggcaa gagcagtagc
     7261 gctaactttg agtggtcagc caaccgctta cgacctcaga caaaagataa cagcagcagt
     7321 cattatagac tactttttag gaactgtagg gctgacagaa gatgaagcgc cattcttgga
     7381 acaaaatacc ggttcgtgtt caggagaatt
//
LOCUS       DAYLZO010000126         7349 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.129, whole genome shotgun sequence.
ACCESSION   DAYLZO010000126 DAYLZO010000000
VERSION     DAYLZO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.129"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..209)
                     /locus_tag="ACGVJG_002564"
     CDS             complement(<1..209)
                     /locus_tag="ACGVJG_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFJ6558474.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPT"
     gene            complement(213..623)
                     /locus_tag="ACGVJG_002565"
     CDS             complement(213..623)
                     /locus_tag="ACGVJG_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFJ6558475.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(620..1543)
                     /locus_tag="ACGVJG_002566"
     CDS             complement(620..1543)
                     /locus_tag="ACGVJG_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFJ6558476.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(1540..2418)
                     /locus_tag="ACGVJG_002567"
     CDS             complement(1540..2418)
                     /locus_tag="ACGVJG_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFJ6558477.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(2405..3742)
                     /locus_tag="ACGVJG_002568"
     CDS             complement(2405..3742)
                     /locus_tag="ACGVJG_002568"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFJ6558478.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(3745..4848)
                     /locus_tag="ACGVJG_002569"
     CDS             complement(3745..4848)
                     /locus_tag="ACGVJG_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFJ6558479.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(4922..6430)
                     /locus_tag="ACGVJG_002570"
     CDS             complement(4922..6430)
                     /locus_tag="ACGVJG_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFJ6558480.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(6396..7088)
                     /locus_tag="ACGVJG_002571"
     CDS             complement(6396..7088)
                     /locus_tag="ACGVJG_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFJ6558481.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(7110..>7349)
                     /locus_tag="ACGVJG_002572"
     CDS             complement(7110..>7349)
                     /locus_tag="ACGVJG_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFJ6558482.1"
                     /translation="LILKIGQTLDLGFEHMYLLLNSLNRSVAEIFDTYIYTAGLKNGQ
                     LSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
BASE COUNT         2128 a         1623 c         1220 g         2378 t
ORIGIN      
        1 tttgtaggat ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag
       61 ttgactaaat ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca
      121 ataatgtaat accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa
      181 ataggatttt tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga
      241 tataaactga atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga
      301 gtgccaatca acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat
      361 tttcctttag cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca
      421 acggcaaaca gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt
      481 gaatgatcta cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt
      541 aaagtaactg ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc
      601 aatagttccc attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag
      661 gtcttttgat cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat
      721 ttttcttgtc atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg
      781 cttcaaataa gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat
      841 caggttctgc agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg
      901 tagatgctgc tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat
      961 gaaaaacggc atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt
     1021 cataggtctg acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat
     1081 ctttttgcaa atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac
     1141 agttttttaa agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt
     1201 ctcctggacc tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta
     1261 agaaaaaagt agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg
     1321 caaaacgatt accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc
     1381 tgctttctat atattcatca tatcttcctt cttttactat caagaccttt ggatggctat
     1441 ccggcaggcc tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat
     1501 ctatagcatt ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa
     1561 ttgtttccaa atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga
     1621 agaaaagttt tctggactac ccattgattg atactttttg tccagccctt ttctcagctt
     1681 cagtacgcct tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg
     1741 cggagcgatt ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata
     1801 gtaataaaac ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg
     1861 tgccgctaga tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa
     1921 taagcctccc atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt
     1981 ctccaaataa tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc
     2041 tgttaataaa aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc
     2101 ccaagcatca atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg
     2161 agaagctaag taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc
     2221 gtgagaatgc aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag
     2281 ggctggtact gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt
     2341 ttgtttttct cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa
     2401 aaaatcattc aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg
     2461 attgatttga ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg
     2521 gtccctcgtg attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat
     2581 aaaatccttg tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg
     2641 catcttgggc catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg
     2701 ctaatccata aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat
     2761 ggatccttct gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat
     2821 acggttcttt ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca
     2881 cattatccat gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc
     2941 tagatttcaa tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat
     3001 tacgaatact accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg
     3061 tgcaatttgt gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg
     3121 ccttgattgc aatacagtca tctcccacgt caatatggca attactaata cgcacatttt
     3181 tgcatgattc tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca
     3241 agttatcaaa cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga
     3301 tcaatttaat atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac
     3361 gaggataagc gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc
     3421 catctaatgt accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg
     3481 aagcataaac ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg
     3541 gatcgtcaga gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta
     3601 aaaataaagc tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac
     3661 ttgctgcagc atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc
     3721 caaatgtcaa aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa
     3781 tgcaccaacg cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga
     3841 accatctcgc tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag
     3901 ccagccttct tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac
     3961 ttgtggaagc cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta
     4021 aacaaacata ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt
     4081 tacttgatac catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt
     4141 ttctagaatt tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc
     4201 tgctgcagcc atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt
     4261 ttttggatca gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt
     4321 ttttggatcg aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc
     4381 tttttcttca ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc
     4441 atccaaccaa atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg
     4501 tggatggttt gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc
     4561 tgtttctttg tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc
     4621 ttcaacgctg tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt
     4681 tatgaaatca aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag
     4741 tacgacgcca tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg
     4801 tgttctttcc atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc
     4861 tttaaaaatt aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat
     4921 attactgtgc cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt
     4981 gtgctttcca gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt
     5041 attgcaccgt agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg
     5101 gtgaatccaa tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt
     5161 gattagagaa ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac
     5221 gagagcttga taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag
     5281 aaccatcatc attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca
     5341 catcttcttc taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt
     5401 ttacttgaga tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat
     5461 gataaccgcc gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt
     5521 gaataccttg acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag
     5581 ttcttccttg tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg
     5641 ccaaatagcc attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg
     5701 tatcaaattc tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa
     5761 aataagaagt gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa
     5821 aaccaactgt gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta
     5881 cttcataaag ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt
     5941 ttcgaatgac taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac
     6001 cgtcgatact cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt
     6061 ttaaatattc tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt
     6121 ttttcaacat cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag
     6181 ttgttaatct ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg
     6241 ttttttcttc gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat
     6301 ttaaccaagt gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc
     6361 tgccgcatgc ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg
     6421 cttttttcat aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt
     6481 tctgtatatg agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact
     6541 gcggaaaaga agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc
     6601 atcagataaa tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat
     6661 ccgaataaaa atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg
     6721 attgggaaaa tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc
     6781 ctaagatacc aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca
     6841 tatatccagc tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt
     6901 ttcgtcaaag gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca
     6961 tacgatgcag gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga
     7021 aaattcaaat aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac
     7081 tgcagcatat ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg
     7141 gcaatcttat tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt
     7201 gctgtactga aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa
     7261 atttctgcaa cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag
     7321 tctaatgtct gaccgatttt tagaatcag
//
LOCUS       DAYLZO010000127         7167 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.130, whole genome shotgun sequence.
ACCESSION   DAYLZO010000127 DAYLZO010000000
VERSION     DAYLZO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.130"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            19..330
                     /locus_tag="ACGVJG_002573"
     CDS             19..330
                     /locus_tag="ACGVJG_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFJ6558483.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            327..557
                     /locus_tag="ACGVJG_002574"
     CDS             327..557
                     /locus_tag="ACGVJG_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558484.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            564..767
                     /locus_tag="ACGVJG_002575"
     CDS             564..767
                     /locus_tag="ACGVJG_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFJ6558485.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            764..1063
                     /locus_tag="ACGVJG_002576"
     CDS             764..1063
                     /locus_tag="ACGVJG_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558486.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            1053..1217
                     /locus_tag="ACGVJG_002577"
     CDS             1053..1217
                     /locus_tag="ACGVJG_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558487.1"
                     /translation="MKFKIFEEDTRYKLEKELNDFAKNNEIQHISLATSKRGYANYYA
                     AVVSYVSREV"
     gene            1306..1719
                     /locus_tag="ACGVJG_002578"
     CDS             1306..1719
                     /locus_tag="ACGVJG_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFJ6558488.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            1732..2118
                     /locus_tag="ACGVJG_002579"
     CDS             1732..2118
                     /locus_tag="ACGVJG_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558489.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            2669..2893
                     /locus_tag="ACGVJG_002580"
     CDS             2669..2893
                     /locus_tag="ACGVJG_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558490.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            2911..3339
                     /locus_tag="ACGVJG_002581"
     CDS             2911..3339
                     /locus_tag="ACGVJG_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558491.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            3317..4735
                     /gene="terL"
                     /locus_tag="ACGVJG_002582"
     CDS             3317..4735
                     /gene="terL"
                     /locus_tag="ACGVJG_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFJ6558492.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            4747..6282
                     /locus_tag="ACGVJG_002583"
     CDS             4747..6282
                     /locus_tag="ACGVJG_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFJ6558493.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            6362..>7167
                     /locus_tag="ACGVJG_002584"
     CDS             6362..>7167
                     /locus_tag="ACGVJG_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFJ6558494.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEY"
BASE COUNT         2495 a         1130 c         1643 g         1899 t
ORIGIN      
        1 attttaggag gagaaataat ggacgaacta atcacaaaag tagagcagtg ggctaaagac
       61 aagggactgg atcaagcaga tccaaaagca cagtttttga aagtagctga ggaattcggg
      121 gaaatagctt cggcgatggc aagaagtaat gatgaactat ttaaagatag cgtaggagac
      181 gttatcgtca cgctgattat cctttccatg caaaaaggga caaacgtaca agagtgttta
      241 gaaatggcat acaacgaaat caaaggacgc acagggaaaa tggtagatgg tgtattcgtg
      301 aagtcgagtg atttggagga tgtgaaatga aatacgaaat accactaagt gaagcgggca
      361 ttcaagcaat tatcaatggt cgggaggtta acatagaact tcctgatggt actgaattag
      421 tcatcagaca aagttatttg aaagatatgg cagctccagt attaattgat cgttttaacg
      481 tgactgattc tgtggtagag aaccacttaa aagaatttcg atcaagtata gacgacactt
      541 tcagattagg gagttgattg acaatgaaca acagacatcg cagaataaca aaactaagaa
      601 aacaggaact gaatgtacta aagacaaagt ttgaaaaaga atatggaatt tcagcagaag
      661 aaacatataa agtggcaagt cagtttgttg ctgatgcaag tgatgctatt cgtaagtttg
      721 ggatttcgat attaaatgat gatcgtaaat gggaggaaat gagatgaaac taaaagacgg
      781 attttacgct agtagtcacg gcatcggcgg tttaatgcta gatatgccga caaagaaccc
      841 taaaacacgt aagaaaccaa aagtcaaagt cggtgacatg gttcgctgtg aagcagaaga
      901 gttcatttat ccgtttcgtg gatatgtaga acacgtctat aatcactcag cgatcattcg
      961 tattgaaaac acaatggaat gcgataagtg gttagcgaaa agcaaagaga atttagctgt
     1021 agcgagattg gtggatatgg aggttataaa caatgaagtt taaaatcttt gaagaggaca
     1081 ctcgttataa attagaaaaa gaattaaacg attttgcgaa aaacaatgag attcagcata
     1141 tatctttagc aacttctaag agaggttatg caaattacta tgcagctgtt gtgagctatg
     1201 taagtcgaga agtgtaactc ggcaaataaa aaaagccgga tcgctccgac tgattcaata
     1261 aatccaacac atttattata tcacataaag gagcggtttg acttgatgca attgttacga
     1321 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
     1381 ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat tacggatatg
     1441 ccgaaggcac cgaagcacgg caataaggca gaagacgcga tcattcaaat gatggatata
     1501 gaagcggaga gagacgcgat tctagtggct ttgatggctc ttagtctgat tagtcgtcag
     1561 atactctact acagcttctg tgtgccagac agcttctcaa actacagaat tagccgtgaa
     1621 gtgggttatt cagaaagaag tatacaacgg atgaaatcgg aagctctaat agagtttgca
     1681 gaagcatata aacacggaag aataattgct tataaatagg gggaccatat aatgtggaaa
     1741 gattatgtat cgttgaaaga gttgaaaaaa gatcttgttt tcaaaagaat tgtagaatgg
     1801 tcagagagtg aattaatttt agaagatgga accaaaatgg aagttgtatg tagcgaatca
     1861 gattgctgcg catgggccga aggtgaattc aaaaatgtaa agcttgatgc agtgattaca
     1921 gatataaaga tttttgataa aggtaaccgt ctctataatg gtgacggaca tacctcttat
     1981 gctgaagtcg ttgtttatca taatcgtaat gagatcgcta aagcagaatg cacagcgaat
     2041 gatgggaatg gcggctacta ttatagtgtt tgtgctttaa aggtcaaaga caaactttgt
     2101 atagtgactg acgcataaaa aaatggcggt tttttggcgg aatgatggcg gtttttagcc
     2161 atttatcagt gatattatgg tagtgtcgaa agattagtga taggtctgag acaaaataat
     2221 aataaaagga acatcgtttt attattgttt cacaattaag cttcgataga cagcagcgga
     2281 aatattaaga ataaggatgt gaattttaac tccttctaaa ttgttcttat tatctatcat
     2341 ccgttgctgt ctattgttac atacagatcg cttaggcggt ctttttattt tggccgaaaa
     2401 cctacatttt cgatggccga ttattggagg aggaatagca tgttcaaact atctgaaatc
     2461 attaagaagt ccgacgttga gaaattagag atgttaaaaa agaagttgaa gaaatagcag
     2521 tctcttcgtg aggctgtttt attttgctca caaaaataga ccactatcgg gtaatagtgg
     2581 tcaggaatta aatgaaaaag atgttagggt tgttagctaa gtataacatc ataacgctta
     2641 caaaacaatg caagaaggat aaattattat gagaacttac tggtatgtgt cattaaacaa
     2701 taaatatccg aagccaatga aaggacagca taggcgtgta gtcatgtctg ttcaaatgaa
     2761 ggcgaagtat tcgattgtag aaatgatcag agaggcaacg ccagtagaga ttgattattg
     2821 caagctagtc tattgtgggt gcggccgttg gaaagaggat catgtacaaa aaaatatcag
     2881 caagtacata tagagaggcg gtgattaagg atggaaaaaa gcgatacgaa agcgaacaga
     2941 tacaaaccta ctgctgcaga gaaaaaactc ttggaagtat tgataaatcc agataactta
     3001 ggcaaaccgg tgcaagagtt gtgttcactg gcgaaaatag gtaggaccaa atattacgac
     3061 gccatgagca aagatggctt tgttgatcta gttaacgaaa caacaatgga tctgatcaaa
     3121 ggaaaagctt ctgatgtcct taatgccact tacaagtatg cattgactga aaaaggtcat
     3181 caggatagga aaatgattct gactattgct ggaatctatg cagacaaaca agaactcaaa
     3241 cattctggcg gcgtcgatat ccgaaaacag tatgagaaaa tgtccgatga agaattagaa
     3301 gagttggtga aacggtatga aaaaatcaac gactcttaat cgtgttgagt atgccgttgt
     3361 tcaaagagag ctagccattc gaaaagctag gaaaagtttt tggctattct gccagcttct
     3421 ttatccagag ttttacaaag atgatcggtt gtatttgaaa gatttatgtc atacgctaca
     3481 agatttttac gaagataaaa tcgacaaaaa aattttggtt attaacatgc cgcctcgaca
     3541 tgggaagact tttacagcgc ggctattcgt tttatggatg tttggacaaa atccaaagac
     3601 gaaaattatt accggatctt ataaccaaat cctgtcatct ttatttgcgc aacaaacacg
     3661 tgatggaatc atggttgaaa acgaaaatgt taaggctgaa tactttaaag atatttttcc
     3721 agaggtgcaa atcaaatatg gagatgctgc aaagggtttc tggtctttag atggttcaga
     3781 agaaaagaat tatttagcga cttctcctgg tggaacatcc actggtattg gcgcgaatta
     3841 tgtaattgtt gatgacatca ttaaaaccgc agaagaagcc tataacgagc gtgttttaga
     3901 ttctcactgg gaatggtata acaacacgtt ggcccaacga atggaacggc cacgtaaaca
     3961 gatattgatt atgacacgtt ggtcaagtaa tgatttagcc ggaaaaatgt tgacgagacg
     4021 aaaaaataat gttcatcaga tctgttataa agcagtacaa gaagacggct cgatgttgtg
     4081 caacgagatt atgacccacg atgagtactt agatgtggta caagaaatga atgtagatat
     4141 tgcagaagcc aactatcagc aagaaccaat tgaccaaaaa ggcaggctat atcagaagtt
     4201 tcttacttat gatacgttgc ctgataacat tatcaaaata tggaactaca ccgacaccgc
     4261 cgacaagggt gccgattact ttgcgtctcc tgtttttgct gagacgtctg atcatgatgc
     4321 ttatattatc gatgtcatgt atacaaaaga gccgatggaa acaactgaaa tagcccacgc
     4381 taatatgatt attcgcaatc gagttaataa ggtacggatt gaaggaaaca atggcgggcg
     4441 tggattcaga agaaattcag aaagaattac aaaagagcga ggctatcgag gtgcagtatt
     4501 tgaagacttt catcaatcgg ataataaact gtctagaata ctggcgaata gtgcttgggt
     4561 ggaaaatcat gtttactatc cagatgattg ggcgacacgt tggccggact tttatgaggc
     4621 tatggttagt tatcaacgtg aaggtaagaa cgctcatgat gatgctccgg atgcagttac
     4681 aggaattgca gaaacgatta tagaagcaga tgacttcgag tttattgctt attaggaggt
     4741 gggtctttgt tttattctgg aatcacattg aaaaaataca aacgtttaag aacgaaatat
     4801 tcaacgcaaa tcgaagaagg attattcgat cctaacgctt ttatggaaga tatgaaaccc
     4861 tttttcgatg atcgagaaag aaagtatctt gcttatacta gcgagaaaaa cgagatcgac
     4921 agtcgtgaga aaccaaaaac aaaaatcatt aaggtcaata acaagattca tgcgggaatg
     4981 tattcagtgg tcgttgatca agcagttaac catttcacag gaataccaat taaatgggat
     5041 tatgatgttt cggaacagaa aaggacattc attcaacgaa tgagagacaa attactgggt
     5101 aatgatatca gcgccccttc taccccagaa gaatttgaaa cactcacgag acaattaagt
     5161 actatgcggt ttggaatgtt agaccccgat actgcaacat ttcaaggagc gacagggctt
     5221 gctttcagat tactggagcc tgttgagaca aatgatggtt gggaactaag agcaagcaac
     5281 attgagccgt ggaaagctga aaagtacgaa aacggcgcag tgtacattaa agagaaatac
     5341 gatccatatc aaaagaagtt ttatgaagag atgaaggttg tcacgccgaa atcaatattt
     5401 acttatgctc gatatactga ttaccctggc acggtacaag gagcatttaa aacgctgggt
     5461 aatgttccta atcctttagg cgttatcaat cttacggagt ttaaaaacaa tacaaaccgt
     5521 tactgtgact ttgaagttgc agaggagatt agtgatgcta ttgatcgtgt tttatctgat
     5581 cagcagaacg agattgaaca attcaagtta gcttatatgc tgatcactgg ggcagatttg
     5641 tccgaggata aagccaagaa gatgatggag caactaggga tcatcaatct gaaagaccct
     5701 aatgcgaaag ccggctacgt gacaaaggat ctacaaaagg atttcaacga gtatcatttt
     5761 gatcttctta aaaagcaatt ctacacggtg tgtaaagcaa ttgatttcaa tgacgaagtc
     5821 tttaagtcga atagttctgg ggaagcccga aagtggcaaa tcattagttt agaagctaag
     5881 acaaatacca aagaacagta ctttcgagaa ggattgaaag agtgtgccga ggttatggct
     5941 gcttatttgg attttgccga gaaagttaca attgatccgg aaaagatcat tttcaccttc
     6001 tctcgttcgc ttccaacaga tttaagctac ctagctgagg cattgcctaa gttggcacca
     6061 tacgtttcta agaggacgat tcaaagtcag atcccgttcg tcacagatgt tgattatgaa
     6121 aacgaaatga ttgacgtcga aagcagacaa tcttacccag acggcgagta tggaggtggc
     6181 atcgttgagt caaatagaaa cgaagtactg gaccaaacgt cgagaactgg aagatttagc
     6241 aagactcaaa atggaaaatc aaacgctaaa gaaactaagt agtgtctttc ctgatgcgtt
     6301 gaaagaaatt caagcggagt tattatccca agctgattta cacgatatta cctatgctga
     6361 gatgatggaa ttttatagta caagtgatca gaaaaaatat cgtgaatacg ttgaaaagaa
     6421 ctacaaatca ctgaagatgt atgatgcaaa atacaaagag ttcatcgaag aatactttcc
     6481 ttctttcgac tacgccaaag tgaatcggct gcttcaaatg aggtcagata tttttcgaat
     6541 tcttgcagag agtgcaatag atgcagatgt gaataaatat ttttcggatc ggttagaaga
     6601 tatcttgcaa cgttcgtatg tatcaaacgc aaacgtattt gcccagattt tgaacgtaga
     6661 tttgccgaat tacttatcaa aggaagaact tgaacgttac ctgaactatc cttggtcagg
     6721 taagacattc tcgcgtcgtt tatggggcaa tatttcatct ctagaacaaa agctatctaa
     6781 cgccattaca actagtgtag caagtggcga aggagttctc gtggcattac gaacaatgcg
     6841 agaagaccca gaaatatgcg atatgttcaa aagggaggaa tcgaagtaca acaaggcgat
     6901 cgaaaatctt gtgagaacag aatacgctaa attcgctcaa gatggtattg agaaatctta
     6961 tattgaaact ggcgtagacg aatacaatgt cttaactgca aaagatgaaa aggtatgttc
     7021 aatatgcggt ggcaaagcaa agaacaaccc atacaagttg agtgaagcga tcattggtga
     7081 gaaccgtgcg ccttttcatg gccgatgcag atgtacagat gttcctaata tgccgaaatt
     7141 aggaaaagat attgacgaag agtatga
//
LOCUS       DAYLZO010000128         7039 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.131, whole genome shotgun sequence.
ACCESSION   DAYLZO010000128 DAYLZO010000000
VERSION     DAYLZO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.131"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACGVJG_002585"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACGVJG_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFJ6558495.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2945..3379)
                     /locus_tag="ACGVJG_002586"
     CDS             complement(2945..3379)
                     /locus_tag="ACGVJG_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558496.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3396..4601)
                     /locus_tag="ACGVJG_002587"
     CDS             complement(3396..4601)
                     /locus_tag="ACGVJG_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFJ6558497.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5295..5681
                     /locus_tag="ACGVJG_002588"
     CDS             5295..5681
                     /locus_tag="ACGVJG_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFJ6558498.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5775..5965
                     /locus_tag="ACGVJG_002589"
                     /pseudo
     CDS             5775..5965
                     /locus_tag="ACGVJG_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6479..6811
                     /locus_tag="ACGVJG_002590"
     CDS             6479..6811
                     /locus_tag="ACGVJG_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFJ6558499.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6721..6915
                     /locus_tag="ACGVJG_002591"
     CDS             6721..6915
                     /locus_tag="ACGVJG_002591"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFJ6558500.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
BASE COUNT         2322 a         1236 c         1005 g         2476 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtgtctagta ctctacatga
     2941 ataattaatt gctttcactc aaatacttat aatctattaa cttgtagttg tttaactttt
     3001 ctgaaaaagt tagacccacg taataatctt cccagctttc tctttcaatt aactgatcca
     3061 atttttgata atccataaat tcatcatgta tcaaaaagaa taaataactt gatattttag
     3121 catctagctt aatcagatta aataatgttt gtttaaaatt gctaccatta atatgactga
     3181 tagattctct agcttcttca cataaacttt ttactttatt aattactact ttgtcatatt
     3241 catttaacac actattatta ccattactta gacgctcaga ttctacgaaa caactcaaat
     3301 cagaaatttc ccaaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3361 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattgtcc
     3421 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3481 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3541 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3601 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3661 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3721 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3781 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3841 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3901 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3961 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     4021 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4081 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagattttct
     4141 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4201 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4261 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4321 aaaactttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4381 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4441 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4501 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4561 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4621 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4681 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4741 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4801 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4861 aggaattttt cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat
     4921 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4981 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     5041 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5101 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5161 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5221 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5281 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5341 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5401 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5461 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5521 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttgata
     5581 aaactgataa agaaattgga aaggttctgg ggattacaag acagggtgcg acaaatttaa
     5641 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5701 tttgtatgct tctttgctcg ttctcctcct tcccaaagaa atagattata agtccatggg
     5761 aagaaggtag atttatgaat tctttaaaag aagatttcat tttagcaaag gctggaaatg
     5821 aagaagctgt tgaggcaatt ctcaaaagat ttagctcttt gattcataag caatcctgag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgtta
//
LOCUS       DAYLZO010000129         6861 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.132, whole genome shotgun sequence.
ACCESSION   DAYLZO010000129 DAYLZO010000000
VERSION     DAYLZO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.132"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..260)
                     /locus_tag="ACGVJG_002592"
     CDS             complement(<1..260)
                     /locus_tag="ACGVJG_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558501.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVA"
     gene            493..615
                     /locus_tag="ACGVJG_002593"
     CDS             493..615
                     /locus_tag="ACGVJG_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558502.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            731..2038
                     /locus_tag="ACGVJG_002594"
     CDS             731..2038
                     /locus_tag="ACGVJG_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFJ6558503.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            2159..3358
                     /locus_tag="ACGVJG_002595"
     CDS             2159..3358
                     /locus_tag="ACGVJG_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFJ6558504.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(3385..4653)
                     /locus_tag="ACGVJG_002596"
     CDS             complement(3385..4653)
                     /locus_tag="ACGVJG_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFJ6558505.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(4709..4775)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(4823..5464)
                     /locus_tag="ACGVJG_002597"
     CDS             complement(4823..5464)
                     /locus_tag="ACGVJG_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFJ6558506.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(5873..6376)
                     /locus_tag="ACGVJG_002598"
     CDS             complement(5873..6376)
                     /locus_tag="ACGVJG_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFJ6558507.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(6499..>6861)
                     /locus_tag="ACGVJG_002599"
     CDS             complement(6499..>6861)
                     /locus_tag="ACGVJG_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFJ6558508.1"
                     /translation="MAGVLLGKGTADIYSTKVLRSMQGSNYHLPVLQKDLTEVIPLMK
                     DKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQEILSLTDQNVYIPMKGNA
                     ESLNVGVAAGIVLYHFSL"
BASE COUNT         2080 a         1460 c         1199 g         2122 t
ORIGIN      
        1 gctacactcg tagttgcact acgccaaaac gaaggatctc ctaacaaatt ttcaattttt
       61 tcttggaacg gggaccagtc atgttcggaa tattttttct caaaacgttt ctttgcttct
      121 ttcgcaaaat ttttgaattt gatctgatct ttttccacat cttgatgggc gacatgcgta
      181 ttcagcatga tcgtgacgaa tggtccttta gcgccttctg aagtcaattg tgacaataat
      241 tctttatcct tttttgccat aaacgtaccc ctcctaaaaa aatcattcct ttgtttctat
      301 ctaaagtcta ccacaacact atttttccat acaataaaac gtatctaaaa aaataaaacg
      361 gtttctacgg ttttaaattt agctattttt ttaagttatc ttcataattt cctcaattat
      421 ttagccccag tcttcaacta ttcttcatga aatgctgata aactaagaaa aagaataaaa
      481 gaggtggatc ttatgcattt gattatgatt ttcagtttat taagtattat cagtttattt
      541 gctttcagcg cttcccgctt attagatgcg gaaacacagc ctattcctgt aaaagtacgt
      601 aaaaacgacc gctaaccttc cataacgtgc gttcttatcc taaagttttt tcagaaacat
      661 tgaaactctc tcccctctct gataaaattt taatttgtag cgaaacaaat taattacaag
      721 gagatcggat atgtccataa aaaaaacacg tctagtccta ttattggcta gtctcattct
      781 ttgccttgct tcttttctac cagctgttgc tgtgcaagct gaagatactt tcaaagtaaa
      841 tgcaaaagca gcctttgctg tagatgcgca aagcggtaag attctttatg atcaagatgg
      901 agaaaaaacc atggggatcg cttcaatcac aaaaatcatc ggtctttaca tcgtccttga
      961 tcaagtaaaa gaagggaaac tatcatggga tgataaagtt tcaatttcag attatgcaga
     1021 aaatttgagt gtcaccccag atttatcaaa tgttcctttg cataaagaaa acacctacac
     1081 cgttaaagaa ttattcgact ctgcaatcat ccagtcagcc aacgcttcaa tggttgcctt
     1141 ggcagaaaaa atctctggca gcgaagcaaa attcactgaa cgaatgaaag aacaattgaa
     1201 agattggggt atcaaagatg caacgatcgt taatgcatca ggactgaata actcttatct
     1261 tggcgaaaac cgaccagaag gtactggaga aaacgacgaa aatcaaatgt ctgctcaaga
     1321 tgtggctatc gttgcccgtc atttgatctt ggattttcca gaaatactgg atgtatctag
     1381 cactacgaca caaatgtttg gcgaaaacac gcaatcacca gtagaaatgg tcaactggaa
     1441 ctggatgctc ccaggattcg tgaattataa agaaggcgtg gacggattaa aaactggtac
     1501 gactgaactt gcaggagcct gtttcgtcgg tacgatcact aaaaacgatc aacgaatcat
     1561 cactgtcgta ttgaatgcag acggccatgc ggaaaaccca agtgcccgtt tcaatgaaac
     1621 agctcgctta atggattatt gctacgacaa ttggtcagaa aaagagcttg gaaaagccaa
     1681 tgcaagtatc cctagtctaa aaaccattga tgtaaaagac ggaaaagaat caacagtgcc
     1741 cgttgtgctt aaaagtcctg taaaagtatg ggtacggaac gatatggata ctggaaaatt
     1801 aacaatcaca ccaactcttg acaaaaaaca agtcactgac aatgagatcc aagcacctat
     1861 ggataaagga acaaaaatcg gtaccgcaac gatcaccttg gcagaagata aattaggtta
     1921 tcttgaagac aacaacgcgc catccacaga aatcatcacg gataaaaccg tagaaaaagc
     1981 aaatattttc gttattggct ggcgacgcgt cgcagaattt ttcggtaact tgttttaatt
     2041 gaacaaaaag aaaaaagcat ctttccatgt ggaagatgtt tttttgcttt ttctagttta
     2101 aataacagct tttttcatct attttcgtta tactgtataa gaaaaaggag ttgaatttat
     2161 gaatctaacc agagatctga tttttttggt tctctatctt attggggtat tctccatcgt
     2221 ttattggcga aaacagaaaa aggatccttc cttgttcttt tacagcagta catggacgtt
     2281 tgcggtaatc ttgacttatt ttgtcgtgtt ggaaattttt tattcgacct tttatttttt
     2341 gattccctta gtgtttttca gtatctttgc cttttcctat ttttcagaaa agcgaaggct
     2401 gttgaatggt cttttattca atattttcct cattagtttt ggtatctact tgtttgtctt
     2461 gctatacgaa acacaaaata tttttcttgg cggactgatt gccttgatca cgatcccgtt
     2521 actgcttgtc tttctattcg gcatttatgg attgatcgtt tttctttttt ggaacggtgt
     2581 cacagtttta cgtagagagt cgcattcatt agctaatctt ttaacgttga tactagccat
     2641 ttttcttaca ttgtttcttg tttttgactt tttcttgtta aaatatttac cacaatggat
     2701 aaatgctttg tttttctgca tcccattgat cttgatctat ctatttatcg tattctacaa
     2761 ctttttgact gtctcattct tgtatcagtt caaccgccct cgatacaacc aagacttcat
     2821 cgttgtgtta ggcgccgggt tgatcaatgg agaaacagtt tctcctcttc ttgccaagcg
     2881 aatcaataag gcgattgctt tttattgggc acaaagccgt gcgacattaa atccgcctat
     2941 cctattaatg tcgggtggac agggagcaga cgaaaaagta ccagaagcaa tggcgatgaa
     3001 acaatatgcc atggaacaag gaattcctga aagagatatt cttgtcgaaa ccaattcgac
     3061 aaccactttg gagaatatgc gctattcaaa ggaaatcatg gatcagcaaa tgaaaggtcc
     3121 ctatcgcgcg atcttcagtt ccaacaatta ccacattttc agagctggtc tctatgcacg
     3181 acaagcgaaa ctgaaggcaa atggcattgg ggcaaaaaca gctttctatt acttgcctaa
     3241 cgcgttttta agagaataca tcgccatctt gatgatgaac aaaaaaagac atatgatcat
     3301 ttgcggattg cttttcgcag gttccgcatt cttatcactg atcactttac ttttctaaaa
     3361 aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt tcttttattc tcttgacgat
     3421 tggcaagaat agtattcctt ttttgacggc tacttgctgt gcagaataaa taccgctgtt
     3481 tcctgaacac ataaagctga tcaagcagat cataaagaag taagcagcag cttgtgaacc
     3541 aaataattcg attcccatca caaaacaggc aatcggtgta tttgtcgcac cagaaaatac
     3601 cccgataaaa cctaatccag caagaaaagg aacagaaaca tggagcaaag gcgctagtac
     3661 tgcgccaagc gttgcaccaa tctcaaatag tggtgtcact tctccccctt ggtaccccgc
     3721 acctaaagac aagaccgtga agaataattt cccaataaaa tcataagcat gtgcctgtcc
     3781 attaaatgat tcctgcagta acggaagact caacccaagg taacgttgtg tttgcaaaat
     3841 caacgctgcg gctacaacta ctgctccacc tatcgtattt cgccaaacag gatgaggaat
     3901 ccattgcgca taccgcgctt ttaaaaagac gatcgaacgg ctgaataccc aaccaatcaa
     3961 accaaaagca atcccagcaa caaaaacttt gaagaacagc aacatactcc attcaggaat
     4021 cacacccatt tggtaatgta gatgagaaac accatagctt tctgtcatga aatttgcaaa
     4081 aagcgcagca aagaagctag gaaatattgc ttctgttcgg actttaccga ttgccaacac
     4141 ttctaatcca aataatgttc cagctaaagg tgtgccaaat acagaactaa aacccgcact
     4201 gattccactg ataaccaaaa tctcccgttc tgctttatcc aatcggaata aatgcgctat
     4261 attatcagct aatgctcccc ccatttgaac tgctgttcct tcacgaccaa cagatccccc
     4321 aaataaatgg gtagtgatgg tcccaaacaa ggtcaatgga atgagccgca aagggatctt
     4381 ctcttctcct ccattccctt gatcgatcac taagttattc ccacgactag aaagaccgcc
     4441 atgatacgca taaagataag caaaaagcgc gccgctgact ggcaatagat aaagtagcca
     4501 aggatgtgct agtcggatat ctgtcacgaa cgtcaggcta tttaagaaaa aagcagacaa
     4561 gctccccatg aatactccga gaatcgctgt gaccaccagc cacttcaaca tgtacatcaa
     4621 aatacgcact ggactcggta attgtttttc cataaaaaat tcctcctcaa ataaaaaaac
     4681 gcctactaaa gaaaggggct ggccccagac tttagtaggc gtcattagct atcttacgtt
     4741 agcatttaaa ggcgaacacc atcacctatt cacttccatt aaaagatagc atatttttct
     4801 gtaaaaaaca ataaaattat cgttatttct ttacacgttc agcaaattca tagaaaggag
     4861 ccaatttcgt taactgttga ttacattgat cactatcatg acaaatataa ttcccctttt
     4921 tcgtatacgt gccatcagaa ccggctttcg ttgtagaaag aaagagcgag acattcgatg
     4981 ttttctgaca gatcgcacag attcccttat taatcgttgg tgataaagta ccgctgatcc
     5041 cttttaattt tccatcttcg taataaacca tgaatttttt ttgcgtccct ggatcattcc
     5101 aaccgatgta cgtatgtgct tgcaagtcga tttcattcca agcgggttgt ttgagttttt
     5161 tcacttttcg gaataatttc tcgatttgtg gtttgctcgg ctgcataaac ggctggacga
     5221 tttgcttcag ctcctccaat gctcgttctg cttgcgtttt tgtcagtttt acatctaaaa
     5281 ctgtcgttaa aaacgtatcg atttctgatg gttcgtaacc taatacatcg cgaatctttt
     5341 cagctgctag agcctgaacc gtttgtaccg tttttgagtc attgacagat gagtacgcat
     5401 ttaataactg ctctacttct ctttttataa aaaaatattg atatggtttc attgtttgtt
     5461 ccatgatttt aaagtccttt cttctaacca aagtgcatac tttgtagaaa aaagtgatct
     5521 tctgcaaagt atgtgttacc tgaagatccc ggactctttt aaataagata cgttttgcat
     5581 cttatcatca ccccctttaa tgttcattct tctatgtaca tgattcattc gataaataaa
     5641 agtaaaccgt atcttgagca gatagtcaac tgcaaacaga aaaaggactg tgacaaaagt
     5701 cgtgtcaaag tccctttcta caaacaaacg gtgttccaga agtagcttct tcaaaaaata
     5761 agccggaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
     5821 gctgacccac ttctgtcaca acctcaatat ggtctattat tggttttacc tgttaaatcg
     5881 aggattcttt tccgaagtaa cgttgccatc tcttacgcat acttgctgac ataggctgag
     5941 cggcttcttt gactgcgcag tatttgtcta cgaatgttac gatatatcct tctttgtact
     6001 taggcggtgc aatcgttgcg ccccacatgt gcttcaaaat gatatctcgc tcaagatccg
     6061 acaaatcggt gatcttttct gcattcttta cagcgattct tggatggata tacgcatgtg
     6121 tgccttcatc aaatttcgtc gtacgccaat catagtagaa cagatcatgc aaaagtccag
     6181 cacgcgctgt tgctttagca ttgccacccc atttttttgc gagcagataa ctatagtaag
     6241 aaacactgat ggaatgctcc aaacgagtgg aatgaacatg ctgtgtgtaa ttcgctaatt
     6301 ttttcactgc ttctgtttct aaaagatctt ctacataaga catatattct tcgtcttcac
     6361 gccatttttc tgtcataatt atcgatctgc cacctttttg aatttcattt tcttctgaaa
     6421 ttgatctaag tataccacca gtgttcggat tattctagtc gccttggata aaatagccaa
     6481 agcttaatag ttccttaatc ataaagaaaa atgataaaga acaatccctg ccgcaacccc
     6541 aacattcaac gattccgcat ttcctttcat cgggatatat acgttctgat cagtcaaaga
     6601 caagatttcc tgacttaccc cctgaccttc gttccccatg attattgcga agttatctga
     6661 tttttctact ttgtctaagg cgtgtgcttc ttcatttaat tctgtgccat acactgtaaa
     6721 ccctttgtct ttcattaaag gaatcacttc tgtcaaatct ttttgcaaca caggcaaatg
     6781 ataattgctt ccctgcatgc tgcgcagcac ctttgtacta taaatatctg ccgttccttt
     6841 gcctaataaa acaccagcca t
//
LOCUS       DAYLZO010000130         6860 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.133, whole genome shotgun sequence.
ACCESSION   DAYLZO010000130 DAYLZO010000000
VERSION     DAYLZO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.133"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1151
                     /locus_tag="ACGVJG_002600"
     CDS             <1..1151
                     /locus_tag="ACGVJG_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFJ6558509.1"
                     /translation="LFDIPLVIAVFITVLDVFVLLLLTKVGFRKIEALVVCLIFVILF
                     VFVYQVGLSNPDWHSLFAGFIPNSHTFSDSPVIGGETPLTGALGIIGATVMPHNLYLH
                     SAVSQTRKIDHQNEEDVAQAVRFSTWDSNIQLSMAFLVNALLLIMGVAVFKTGSVQDP
                     SFFGLYEALSDPSAMSNGILIAVAKSGILSTLFAVALLASGQNSTITGTLTGQVIMEG
                     FIHMRIPIWLRRLVTRLLSVIPVLICVLFTSTKGTIEEHAALNNLMNESQVFLAFALP
                     FSMIPLLMMTNSQAEMGSRFKNSFIVKLLGWFSVISLTYLNLRGLPGQIESFFGEQAT
                     SAQLALADHIAYVIIVLVLLLLAWTIGDLYKGNKKYEQLLQETVEEIE"
     gene            complement(1217..2992)
                     /locus_tag="ACGVJG_002601"
     CDS             complement(1217..2992)
                     /locus_tag="ACGVJG_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFJ6558510.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3203..4105)
                     /locus_tag="ACGVJG_002602"
     CDS             complement(3203..4105)
                     /locus_tag="ACGVJG_002602"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFJ6558511.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            4384..6729
                     /locus_tag="ACGVJG_002603"
     CDS             4384..6729
                     /locus_tag="ACGVJG_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFJ6558512.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         2116 a         1355 c         1325 g         2064 t
ORIGIN      
        1 tactttttga tattccattg gtgattgccg tctttattac tgtattagat gttttcgttc
       61 tcttactact gacaaaagtc gggtttcgta agatcgaagc actggttgtc tgcttaattt
      121 tcgtcatttt attcgttttt gtctatcaag ttggcttgtc taacccagat tggcacagtt
      181 tatttgctgg ctttatccct aacagtcata cattctcaga ctcaccggtc atcggcggcg
      241 aaactccgct gactggagct ttagggatta ttggtgcaac agtcatgccg cataacttat
      301 atcttcattc agcagtttca caaactcgga agatcgatca ccaaaacgaa gaagatgtag
      361 cacaagctgt acgtttttct acttgggatt ctaatatcca gttgtcaatg gcttttctag
      421 tcaatgcact gctattgatc atgggtgtcg ctgtcttcaa aaccggttct gtccaagatc
      481 cttccttttt tggtctgtat gaagctttat ctgatccatc agctatgagc aatggtattt
      541 tgattgctgt agcaaaatct ggtatcttgt ccactttatt tgctgtagca cttcttgctt
      601 ctggccagaa ctctactatt actggtacgt tgaccggtca agtcatcatg gaaggattca
      661 tccatatgcg tatcccgatt tggctaagaa gactggtcac acgattgctt tccgtgattc
      721 ctgtattgat ttgtgttttg tttacaagta caaaaggaac gatcgaagaa catgccgctt
      781 tgaataactt aatgaatgaa tcccaggtgt tcttggcttt tgcgttgcca ttttcgatga
      841 ttcctttact gatgatgacc aacagccaag cggagatggg tagccgattt aaaaattcat
      901 tcatcgtaaa acttcttggc tggttctctg tgattagtct aacttacttg aacttacgag
      961 gattaccagg acagatcgaa agtttcttcg gcgagcaagc cacttccgct caactagcat
     1021 tggctgatca catcgcttat gtaatcattg ttcttgtcct tctcctgttg gcatggacga
     1081 ttggtgattt atataaagga aacaaaaaat atgaacagct tttgcaggaa acggtagaag
     1141 aaatcgaata aaaactgatt tttccacaca aagacccttg atagttaggt taaaaatcta
     1201 gctaccaagg gtctttttat ttagaagata aagctttttt cagatttaat ttactgaaaa
     1261 ctagcatgct tgttactacg atgaattgcg ttaatggcac gcctgcacca gtgtagacac
     1321 ttaaaaaata gtcctttttt tctggcgcat aagaacaaaa ccatgagaca ccgaaaagaa
     1381 aaaccaattg tgtcagtatt aatccggtga ccacaatcaa agaggaacta taataggcac
     1441 gatcagcttt tgcaaatacg gttcctagta tcataagtaa tatccctgct attgaagcaa
     1501 tcagcacggg aatagccaga acttggtaaa tttttatgat gacattcgcg acttttgctg
     1561 attttgttaa ttggaaatta ggtgttctcg gttcattact atcgatccaa ttctccatta
     1621 gcggaaggtt taataattct tgagcctgtt tgacttgttc ttgcgttccg aaactataat
     1681 ttcctccttg atggaaatct ttatatcgcc atgcataagc gtatcctgat tgaaggaaat
     1741 cgaatacgat tggcagatca ctcatcgtct ttccatctcc tgatcccatc agatagaact
     1801 cttttttctt tttgatggtt cccttgtgat aggcttcttc taattcttgg ttgacttttt
     1861 tccaaaaatc attggtttct tttgcattgt tttggtaata accagattcg cttaccacat
     1921 ctcgcaatgc ccaaaagatg atatctccgg gtaattcttt tacttttcca ccttgagtcc
     1981 acgcgtgagt cgtatataat tcgtcaatac gggtagaaac agtatggaat gtaggagatg
     2041 cggctaatgc tttatttagt gcagcatgtg agacccatat ctcactgttt tctttattca
     2101 agttcgtttc atcatctatt cggatcagtt gtttcatcaa acgtccaaat tctccactgg
     2161 aacggtcatt gatggttgca ataccgtatg ttgtctcatt tttatattcg atccactgat
     2221 agacgatgga aaagccaact ataggaagta aaaacaatga aagtttcagc cattcccgtt
     2281 tcgagagaag aaagagtcgt ttttttaact tctttacttc tttgaaagaa aagtcttgcc
     2341 ctagcaagat ctggacgaaa ctgataccca aagctactac agtaaaagga agaagccaga
     2401 tagaatcttc gcgaatgaac caaaagaaag gaaaagcgac agaaagccct aaaaaccatg
     2461 gcagcaattt tctgctagaa ccgtatttgc ggtaataaat agctaataaa caagcgataa
     2521 tgatctcaac agccggaatc acgagggcat tacgatatgt tcgagtggaa tattcatgtg
     2581 taaaactaat cggagaaaac aaaaaataaa aatagatact taaaagaaga tacttgttct
     2641 tcacaatcgg tttaaacgcg agtgcaacca atatactggc aaaaatactg aacatgccga
     2701 acaaaactgc ataagaaaga tgcagtttat gtgcaagtac taaaaataag ctgtaggaga
     2761 ttcctttgga aagtgttgta gtcgtatact cccctagcca tttaccatcc aaaatatttt
     2821 gagcatagtg aatcagcagc tgatcatcat aggtcgccga catatctgtt ccatagattg
     2881 ctttactata taaatacaac cgaataattg taaataatac ggctatgcca agaataaagt
     2941 atgtatgttt cttttttagt tggtcgatca aggggtttaa aatgttcagc atcttttcgt
     3001 cctttcaatc acgttttgct acttgtttgt gaaacttttc gttttttact tttctatcag
     3061 tataccctgt ttgtctgaat ttaaaaagag ggggcaaaag gagcatggcg aaaacgaatg
     3121 caaaaagcac tcctttgttt cttaaacaaa aaaggacaag gcagactgcc caatcctttt
     3181 ttgttgctgt caggtagaaa acttaaagtg tgacttcttc tgctaataac tcagggaaca
     3241 cttcgggatc gaatgcttgc gctttgaaca tggaaatttc ttttccatat ggtgcgtaag
     3301 ctttcttttt atctttaccg acatatgggg tttctaatat tttcgaaatg ttgtcaaaca
     3361 ttggatcatg gacgattttg ttcaatgtgt caaatccaat tgttccaaaa ccgatattgg
     3421 catgccgatc tttatgagaa ccttgtggat ttttagaatc attgatatgc agtactttaa
     3481 gccgatctaa tccgatgacc ttatcaaact cttccattac acctgaaaaa tcttctttta
     3541 tattatatcc ggcatcattc gtatgacatg tatcaaaagt aacggataat ttttcattca
     3601 attttactcc atcgatgatt tgcgctaatt cctcaaatgt tcgaccgatt tccgtacctt
     3661 tccctgccat cgtctccaaa gcgatctgtg ccgtctgttc ttttgtcaaa acttcattca
     3721 acccctcaat aattctagca atcccagctt ctgcccctgc tcctacatga gcgcctgggt
     3781 gaagggtgat ttgagtagca cctaaagctt ccgcacggac aatctcttct ctcagaaatt
     3841 gtacagcaaa tggaaacatt tctggtttga tcgtatttcc caaattaatg atataaggcg
     3901 catgaacgac gatatttgtt agtccgtttt ctgccataaa tgcttttccg gcatcaatat
     3961 tcatctcttc gatagctttt cttctagtat tttgaggagc gcctgtatag atcataaagg
     4021 tcgaagatcc gtaactggct gcttcttctg ccgcgcctaa cagcattttc tttccgctca
     4081 tgctgacatg tgagcctaat aacatggatg gattcctcct tgaaaggcaa ttgtttcgaa
     4141 caattcctgt atattgataa gatgatgtac aaagaaaatg gtttgttttt ctttgccctg
     4201 ttctccatag taactgaaac tttccctaaa gaaaagctta agaactcttt cataaaaaag
     4261 atcatttcct cacgttttat ctcattcgaa aaagaaattc ctgtcaaaaa cagccttgtc
     4321 tgtttttttg tcctattatt tgctataatt agcgaaataa aaccattagt tgaggtgacc
     4381 cttttgccac gcaaaaatac tcgaaaaaaa cagaatagaa gaaaaaaaga aaaatggttt
     4441 gttcctaaga ttatttttag agtgtttcaa tcattgactg tttttattac tgttcttctt
     4501 ttgttgttcg cagctctagg tatcggaata ggtgctggct actttgccta tcttgtcgaa
     4561 gacacacaat tgccgactaa aaaagaactg cagacagaac taggaaacat tacggaaacg
     4621 tcaaagattg tttatgcaga taatacagag atatcaaaaa tccagacaga tctgatgcgg
     4681 acgactattt catctgataa gatttcgcct ttattgaaaa ccgcgattat cagtacagaa
     4741 gatgaatact tcgataaaca tcaaggttat gtccctaaag ctgttttacg agcactagtt
     4801 tcagaagcga ctggcatcgg ctcgtctgga ggatccactt tgacacagca attagtcaaa
     4861 caacagatcc taaccgatga aacaacattc aaacgtaaag caaacgagat cgttctttct
     4921 gcacaagtag aaaaacactt ttcaaaaaat gagatcatta ctacgtattt gaatgtctca
     4981 ccatttggtc ggaataataa ggggcaaaat attgctggag tccaggaagc tgctagagga
     5041 atctttggtg tagatgcaaa ggatgtgact ctgccacaag ctgcttatat cgctggtttg
     5101 cctcaaagcc ctatcacgta tagtccatac acgaatacag gcgcgctaaa agaagatctt
     5161 tctgccggtc ttgccagaaa agattttgtt ttattcagta tgtaccgtga aggacaaatt
     5221 acgaaagaac aatacgaaga agcgaaagcc tatgatctta caaaagactt tctcccacaa
     5281 caaatcgcgg aacagactga cagagaattc ttgtattata ccgtgatgaa cgaagcaaca
     5341 aggattattg cacaacaatt ggcagaaaaa gataatgctg atatgtcaga ttcatcggtc
     5401 agtgaagctt attatcaaaa agcacagcaa acgatccaaa acaaaggata tacgatccat
     5461 tcgacgatcg ataaagatat ctatgctgca ttacaaaccg gcgtagaaaa ttatggctac
     5521 atgttagatg atggtgctgg tagccaagtg gagactggaa atgtattgat ggataacaga
     5581 accggacgta tctatggctt cgtgggtggt cggaattaca gccagaatca aaataaccac
     5641 gcctttgata cagaacgtca agctggttca tccatcaaac cggttcttgt ttacggtcct
     5701 gcaatcgata tgggacttgt cggatcagaa tcccgtgtat cagattatgc aacgacttgg
     5761 caagaaggaa aaaatgctgg tgagaaaatc gtcaatgcga ccaatgaagg gtcgaacacc
     5821 ttccaaaccg tccgtgagtc actggaatgg tccaacaata tcccagctta tcacttatat
     5881 caagatgtgt tgaattccgg tggatcgaaa caatacgctt acgagcacta cttggcgaag
     5941 atgaactacc cagcaaatga taattggggc gttgaatcgg ctcctttagg gacagtagat
     6001 gtaacgacgc ttcaacagac aaatggattc caggccttag caaatggtgg agtctacgaa
     6061 gaaggctata tcattgattc catcacagat aatgcaggta atgtgattta taaacatgaa
     6121 agtaattctg tccgtattta ttccgaagca acggcatcga tcatgaatga catgatgcgt
     6181 agtgtgatca atgaaaaaat cactacacca ttcaaagatg tcatctcaag cttaaacgga
     6241 aatctaggaa atgcagattg ggtcggaaaa actggttcta ccaatgaata ccgagattca
     6301 tggctgatcg tctcaactcc atctatcact atcagtagtt gggcaggaca cgatgacaat
     6361 actggaatgg acagccaagc aagaatacgt tcggctaatt atcttgccaa tctaatcaat
     6421 caagcttacc aagcaaaccc tgagattttc ggcactgatg agaaatttga cttatcatcc
     6481 gatgtgatga agaaaaaagt atctgggttc acaggacaaa cagctggaaa agttactgtc
     6541 aataaaaaat ccatccaaac gccaggaaaa acagtggagt cactttgggc aaaaaatggt
     6601 ccgaagaaaa gcgcctttaa attcggcgta ggcggtactg atgaaaacta tacagattac
     6661 tggaacaccg cttcagctta tgctagagcg aatccagaga ccaaagaatc agacgataaa
     6721 gatgattgat ctaatagatt aatcagaatt aaagggagct gcgacaaaaa tcttgtcaca
     6781 gctccctttt tccgaataaa tggtgttcaa aagcttacat ctacgatatt aagacaaatt
     6841 tatgtaaaaa tgtgaaaaac
//
LOCUS       DAYLZO010000131         6808 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.134, whole genome shotgun sequence.
ACCESSION   DAYLZO010000131 DAYLZO010000000
VERSION     DAYLZO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.134"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..553
                     /locus_tag="ACGVJG_002604"
     CDS             <1..553
                     /locus_tag="ACGVJG_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFJ6558513.1"
                     /translation="KMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANLITIVA
                     IFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPKRVSII
                     GISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLLRHVQT
                     LQRFQKDYEQGTKQKWSPMFTKK"
     gene            576..1562
                     /locus_tag="ACGVJG_002605"
     CDS             576..1562
                     /locus_tag="ACGVJG_002605"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFJ6558514.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1717..3471)
                     /gene="scm"
                     /locus_tag="ACGVJG_002606"
     CDS             complement(1717..3471)
                     /gene="scm"
                     /locus_tag="ACGVJG_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFJ6558515.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSS
                     TTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVI
                     LLVIGLRITFMSYKKSKR"
     gene            3758..4282
                     /locus_tag="ACGVJG_002607"
     CDS             3758..4282
                     /locus_tag="ACGVJG_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFJ6558516.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            complement(4599..5567)
                     /locus_tag="ACGVJG_002608"
     CDS             complement(4599..5567)
                     /locus_tag="ACGVJG_002608"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFJ6558517.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(5724..6530)
                     /locus_tag="ACGVJG_002609"
     CDS             complement(5724..6530)
                     /locus_tag="ACGVJG_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558518.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            complement(6527..>6808)
                     /locus_tag="ACGVJG_002610"
     CDS             complement(6527..>6808)
                     /locus_tag="ACGVJG_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558519.1"
                     /translation="ADLNSGIGTIVIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYR
                     FILALVFELNVEPSDSKLASAFVLVICLSFPQIQQKFHLPQKKKGGSVK"
BASE COUNT         1958 a         1290 c         1427 g         2133 t
ORIGIN      
        1 aaaaatgatt tatatttcgc tacttgttgc acaaggagtg atcattggtt tattggaaaa
       61 tatgatccct tatccttttt cttttgcgcc tggtgcaaaa ctaggcttag ccaatctaat
      121 cacgatcgtt gccattttta ccatgaaaaa acaagacagc ttcttactac tttggctacg
      181 actattcctg actactttgc taggcggaac gatttccaca ttcctttata gtatgagtgg
      241 tgctttgctt agttattttg gaatgttatt ggtcaagcaa ttaggaccaa aacgtgtcag
      301 tatcatcgga attagtgcgg ccggcggttt catgcataat gtcggacagc ttttgatggc
      361 ttctttcatt gcacaatcat ggacagtcat gttgtacttg ccagtccttt cagttttagg
      421 gattttatca gggattgcaa tcgggatcgc agcgaattat cttttacgac atgtgcagac
      481 acttcaacga ttccaaaaag attatgagca gggaaccaaa caaaaatgga gcccaatgtt
      541 taccaaaaaa taatcgtcac acaaggagtt tttttatgaa gctacatcca atgtgggaaa
      601 actatcctgc tttaagtaaa gaattgacag aaacactaaa acggatggaa gaaactgtcc
      661 gcctgaaaaa caaacctgtc gaacaagcaa tcaaagaaac gatccatgcc ggcggaaagc
      721 tgttgcgtcc tgcctatcag ctattgttct cacaatttgg accagagcaa gatcgagaca
      781 aagccatcgc tttggcagct tctatcgaaa tgctccatac ggccacactg atccatgatg
      841 atatcgttga tgaagcggat ctgcgacgtg gtcagcctaa tatccgtagt cagtttggta
      901 atgtcgtttc tgtctatgca ggagactatt tatttgtctg ctgttttaaa ctattgtctg
      961 attactccac ttccttgaaa agtctccaat tgaattctcg cagtatggaa aaagtattga
     1021 atggcgaatt agggcagatg gatgatcgct ataattatga actttctgta aaagaatatt
     1081 tggaaaatat ttcaggtaag acagctgaac tgttccaatt aagttgttct gttggcgctt
     1141 ttgaaagtgg aacaagcgag cgcttcgcaa aaaaagcagg agatatcgga ctttctatcg
     1201 ggatggcctt tcagattatc gatgatattt tagattatac aaaagagagc caggaaatcg
     1261 gcaaacctgt actagaagac atgcgtcaag gggtctattc tttaccgttg atctactctt
     1321 tgcaaaaaaa caaaccaaag ctactacctt atttagagaa gaaagcggca atgacagaag
     1381 aagatgtcga cgccgtacgc aaaatcgttg aacatacagg aggcgtggaa gaagctagga
     1441 agttagcggc ctcttacaca gaaaaggctt tgaaggaaat aaagaaatta cctgccacct
     1501 ctcttcgaac aaaagagaat ctcttttccc ttacccaact gattttggat cgcaaagact
     1561 gaatagggaa aggttcgata cgtaaataaa tttctcagaa ggaaatctac gtgttttttg
     1621 acaaattgaa ttgtagttct tgtatttatg ataaaaagca aaaataaaga gcaacgtccg
     1681 ttagcgacgt tgctcttttt tatcttgaat atttttttac cttttcgatt tcttataact
     1741 cataaaagtg attcttaaac cgattacaag taatataacg cctgcgagtg ttatccaatt
     1801 attcgattga cttccagttt taggaagtgc tctttttgat ttgtttccat tgtcattgct
     1861 tgttttatta gttaagttaa agttcgtcct gttatccgaa cttgtttttg agaaggagac
     1921 acttgcatga cgtgtatcgc ttgtggtaga cgacgtgtct gtactatcgc ttgaagttga
     1981 tgaatctgag ctatcacttg tggtagagga tgtatctgta ctgtcacttg aagctgacgt
     2041 gtccgtgcta tcacttgtag tggtagatga atctgagcta tcacttgtgg tagaggtagt
     2101 atctgtactg tcacttgaag ctgacgtgtc cgtgctatca cttgtggtag aggtagtatc
     2161 tgtactgtca cttgaagctg atgtgtccgt gctatcactt gtggtagagg tagtatctgt
     2221 actgtcgctt gaagctgacg tgtccgtgct gtcacttgtg gtagaggtag tatctgtact
     2281 gtcacttgaa gctgatgtgt ccgtgctatc acttgtggta gaggtagtat ctgtactgtc
     2341 gcttgaagct gatgtatccg tgctatcact tgtggtagag gtagtatctg tactgtcgct
     2401 tgaagttgat gaatctgagc tatcacttgt ggtagatgta tctgtactgt cgcttgtgtc
     2461 tgtcgtatct gtaccattgt cgctgataaa tatttcgtta ggatcccaac gattggtagc
     2521 tgcatctaaa atcacatttg ttcctataat cgttccgtct tggttttgag taatgcggat
     2581 agtagcatta ggcgcaagga ttgttccttg aaaaggagca ctgattgtca aatcagtcat
     2641 ctcatttcca aaattccaag agatattcgc atccgaaaaa tcttctgttt catgatttga
     2701 acgatcattg tatttgattg gtgactgtac gttcaatgca gaagaactgt tgatgacatt
     2761 aaatacaact acttgatcat ttggattaat gatttgaagt ggcgtattca tcgttaacac
     2821 ttctgcatcg atatttacca ataaaaaccc ggtatctgat aaatcagtta aatcgattgt
     2881 ccgattgttc atgtctggaa aatctgcagc agtatatgtt ttagctggag tgatcgttgc
     2941 aagatcattt gctgctacat tcaatttagc aaattcctgc tcgaaatcaa tatattcatg
     3001 cccagcacga tctttataaa aatcatctgg agttaagtga tcaagcctgg atccattgac
     3061 tgtaggatga ccatcttgaa gtgctacatc cacgttactg ccaacgacaa atttattcat
     3121 tcttgtgcta cctgtaggta tgcctgaaga attagcgaaa gaatcagttt tttgtatata
     3181 atttatttct tggttcaata aaccttcatg aagctctgta ccaaaattat tttttgcatc
     3241 aagttcatgt gttgctatat ttccgtctgt atgtgcattc acagttgtct tatttttagc
     3301 aaatacgtgg aattgactag caattcctaa caagccatag ttaccagtta gatcattata
     3361 aatattccca gggtctttag gaatgtatgt ttccgctgaa acggccacac catacgaaaa
     3421 gatattgaac aacaaagcaa ctaggactag taaacttgcc ctttttttca tttttcccct
     3481 tcctttcctt tcaaacattt tcctattata ctctttttct cctagtctta aactgttcgt
     3541 ttgaaaaatc attgtttatt taaagaatat taatgaaacg ttcatattgt tgcttcttgt
     3601 tcaattgata gttcgacact atctttttct ttcaatctcc aaactattga tgatggtgac
     3661 tgttaaagtg taaaaaataa gggtgtgtag taacatataa gtaggtatcc tagaattagg
     3721 aaaaagtaag taggataaat aaaaaggagg aaaataaatg gattatttag atatgctatg
     3781 cgagagtgtg gatcgggcat acgatcggtt tgaacgtttg tttgaaggcg tctctattga
     3841 ggaggccaat cgttttccag tggaagatag agccccgcag atcaaatcaa tgacttggct
     3901 ggcttggcat accgctcgag aattagattt ccagattgct gatttagcag gaacttctcc
     3961 aatttggcat agtcaagact gggaaagccg gtttccgatg acgatagcag ataacgaaca
     4021 agattggaag catagtctgg aacaagcaaa gcagatccaa gctaatcaaa tagaagatct
     4081 gctaggatac ttgcgaggag ctgctgattt tgctatttct tacttaagaa ggctggaacc
     4141 ttccgagcta gatgaagtaa ttgacgagaa ctggacacca ccagtcacac gaggaatgcg
     4201 tctcgtttct attattgatg atgctgctat gcactctggt caggtgatct atgcgagacg
     4261 tttgttggga ttaagagatt aaggcactag ctaaaaaaga acttacggca aaataagtcg
     4321 caagttcttt tttgttttta tcgttaccca tggtctatct ataagattga aaaaggaagc
     4381 ggcctgctat ctcttttgat cgtgtaaact gatgactctc ttgttttttg cattgattca
     4441 tttaaagaaa gtgtaccatg ctttatagat atttttagaa tacctttttc tagaggatag
     4501 agaaactgtc agctgataga ataaggagag tcttttgaag agaattcaga aaaaaggaca
     4561 ctgtatgaga tcaggtgtcc ttttgtgaaa tctgataatt attgcgtacg gatagatgtg
     4621 agactgattt cgccagaaga caaactcatc ttctgttcat ttgtttggac gatcagttcg
     4681 ccatgatcac cgatttcttt tgcaagaccg acataagtcg ttcctttttg attgaacgac
     4741 acggtttttc ccaatacgaa tgatttttct ttatagaagt ccaacgcttt ttctgggtga
     4801 gaaagataag tgaacaattc ttgccagatt ttgctgatta gctgattcct tgtgacacta
     4861 gcttgtccgt cgggaaagac agagatggct ttttcttgga gatcagctgg aaaatgattt
     4921 tgaggaacag aaaaattaag cccgatccca atgatgatat gactgatcct gccgctttcc
     4981 atatcactga ctgcttcaga taaaatccca cagacttttt ttccatccat gtagatatca
     5041 ttgacccatt tgatcgaagc agaacgaccg gttacagatt caatggcttt cacacaggca
     5101 ctggctgcga tgatcgtata ttgaggcaat tcatcaaacc gtttgtctgg atggagcaag
     5161 agactcatgt agattcctcg tccaggttca gcgaaaaaag gccgtccaaa acgtccacga
     5221 gcttgttctt gtgtatcagc aacaaataac ttcgaaacag aatctccttg agctgctgcc
     5281 agtttggcat ctttcatcgt agattcagaa gtcatcatcg tttgtacttt caaatcagga
     5341 ataaacgacc gtagctcttg ctggatttct tttgcatcta gtacgtcaga gggcaggtaa
     5401 cgataccctg taggcccgtg ctcaaaacga tagcctgcct tttccaattc tttgatggct
     5461 ttccaaatac ttgtccggga aacttccagt tgctgtgcca acttttctcc ggaaagaaaa
     5521 tctggtgtct ttttcaataa ttgaagtacc ttgtctttcg ttgtcatggc atcaagctcc
     5581 tttcccatcc attctatcag gaagaaggag agaacaaaat agttttcgaa gaaacaacgg
     5641 ttgatacata aagcaaaaac gaaagaagga aagagaaaaa agaagagggt gggcgttcat
     5701 tttttgtccc atcctctttc tgattatgaa gataaaagta cttgatcgtc ttttaatgcc
     5761 gtgccgctgt tttgtttgaa caacgctaat aattggggca ctgtgagctg ctgtttttcc
     5821 tcttgctgga tatctacgac gatccttcct tgatgcagca tgatcagtcg actcccatag
     5881 cggatcgcgt cttccatgtc atgagtgacc ataaaagcag tgagattctg ctcagtgatc
     5941 agcttttctg tcaattccat caccgttttg gaagtagcag ggtcaagcgc tgcagtgtgt
     6001 tcatctaata agatcagttc tggacgctgt aatgtggcca tcaataacgt aatagcttga
     6061 cgttgtcctc cagacagcaa accgatttct gaggataaac gattttccaa ccccaaattt
     6121 agccgaacca gctggtcttt gaaaaatgtt cggtcttgtg gtttgacacc acggaagaag
     6181 ccgcgttttt ttcctcgttt catagcaaga gcaagatttt cttctaccgt taaacgaacg
     6241 gctgtcccta gtttaggatc ttggaatacg cgactgatgg atttcgaacg ttcgacaaca
     6301 gattttttcg tgatattttt tccattcaat aaaatttgac cctgatcgac agaaaatgta
     6361 ccggcgatgc tgttcagcaa agtagattta ccagccccat ttccaccgat gatcgtcacg
     6421 aagtcgccgg gatctaagga aagattaatg cctttcagga catgattttc gttgatcgtt
     6481 cctttttcaa aggtatgatg gagattttcc aatgttaaga cgtcattcat ttgacactac
     6541 cgcctttctt tttttgaggt aaatggaatt tttgctggat ctgcgggaaa gaaagacaga
     6601 tcactagcac gaaagcagaa gcaagtttcg aatcacttgg ttcaacattc agttcgaaga
     6661 ctaacgctaa aataaaacga taaatgaccg cacctaaaac gacagtcaag agacggagaa
     6721 atagtggctg gttacgtaac aaaacttctg cgatgataat agaagcaagt ccaattacga
     6781 tcgttccaat accagaattt aaatcagc
//
LOCUS       DAYLZO010000132         6808 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.135, whole genome shotgun sequence.
ACCESSION   DAYLZO010000132 DAYLZO010000000
VERSION     DAYLZO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.135"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..676
                     /locus_tag="ACGVJG_002611"
     CDS             <1..676
                     /locus_tag="ACGVJG_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFJ6558520.1"
                     /translation="NSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFE
                     QYLRYASSFGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMV
                     GIEPGLNHPVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFG
                     EKICAVHLKDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRY
                     GRSLLE"
     gene            841..1740
                     /locus_tag="ACGVJG_002612"
     CDS             841..1740
                     /locus_tag="ACGVJG_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558521.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(1866..2735)
                     /locus_tag="ACGVJG_002613"
     CDS             complement(1866..2735)
                     /locus_tag="ACGVJG_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFJ6558522.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            2888..4348
                     /gene="rhaB"
                     /locus_tag="ACGVJG_002614"
     CDS             2888..4348
                     /gene="rhaB"
                     /locus_tag="ACGVJG_002614"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFJ6558523.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            4345..5601
                     /gene="rhaA"
                     /locus_tag="ACGVJG_002615"
     CDS             4345..5601
                     /gene="rhaA"
                     /locus_tag="ACGVJG_002615"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFJ6558524.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            5615..6460
                     /gene="rhaD"
                     /locus_tag="ACGVJG_002616"
     CDS             5615..6460
                     /gene="rhaD"
                     /locus_tag="ACGVJG_002616"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFJ6558525.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            6462..6773
                     /gene="rhaM"
                     /locus_tag="ACGVJG_002617"
     CDS             6462..6773
                     /gene="rhaM"
                     /locus_tag="ACGVJG_002617"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFJ6558526.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
BASE COUNT         2213 a         1139 c         1542 g         1914 t
ORIGIN      
        1 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
       61 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
      121 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
      181 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
      241 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
      301 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
      361 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
      421 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
      481 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
      541 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
      601 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
      661 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
      721 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
      781 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
      841 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
      901 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
      961 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
     1021 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
     1081 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
     1141 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
     1201 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
     1261 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
     1321 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
     1381 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
     1441 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
     1501 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
     1561 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
     1621 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
     1681 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
     1741 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
     1801 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
     1861 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
     1921 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
     1981 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
     2041 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
     2101 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
     2161 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
     2221 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
     2281 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
     2341 tcccctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
     2401 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
     2461 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
     2521 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
     2581 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
     2641 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
     2701 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
     2761 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
     2821 ttgttaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
     2881 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
     2941 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
     3001 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
     3061 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
     3121 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
     3181 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
     3241 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
     3301 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
     3361 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
     3421 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
     3481 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
     3541 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
     3601 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
     3661 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
     3721 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
     3781 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
     3841 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
     3901 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
     3961 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
     4021 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
     4081 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
     4141 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
     4201 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
     4261 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
     4321 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
     4381 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
     4441 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
     4501 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
     4561 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
     4621 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
     4681 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
     4741 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
     4801 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
     4861 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
     4921 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
     4981 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
     5041 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
     5101 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
     5161 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
     5221 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
     5281 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
     5341 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
     5401 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
     5461 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
     5521 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
     5581 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
     5641 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
     5701 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
     5761 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
     5821 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
     5881 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
     5941 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
     6001 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
     6061 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
     6121 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
     6181 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
     6241 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
     6301 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
     6361 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
     6421 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
     6481 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
     6541 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
     6601 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
     6661 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
     6721 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
     6781 caactgggca accggttgct tggggttc
//
LOCUS       DAYLZO010000133         6576 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.136, whole genome shotgun sequence.
ACCESSION   DAYLZO010000133 DAYLZO010000000
VERSION     DAYLZO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.136"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..426
                     /locus_tag="ACGVJG_002618"
     CDS             <1..426
                     /locus_tag="ACGVJG_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HFJ6558527.1"
                     /translation="GFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKPH
                     PTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKYG
                     SVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            1602..2276
                     /locus_tag="ACGVJG_002619"
     CDS             1602..2276
                     /locus_tag="ACGVJG_002619"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFJ6558528.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            2273..2614
                     /locus_tag="ACGVJG_002620"
     CDS             2273..2614
                     /locus_tag="ACGVJG_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558529.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            2644..3003
                     /locus_tag="ACGVJG_002621"
     CDS             2644..3003
                     /locus_tag="ACGVJG_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFJ6558530.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            3264..3386
                     /locus_tag="ACGVJG_002622"
     CDS             3264..3386
                     /locus_tag="ACGVJG_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558531.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            3528..5612
                     /locus_tag="ACGVJG_002623"
     CDS             3528..5612
                     /locus_tag="ACGVJG_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFJ6558532.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            5903..>6576
                     /locus_tag="ACGVJG_002624"
     CDS             5903..>6576
                     /locus_tag="ACGVJG_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFJ6558533.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYV"
BASE COUNT         2176 a         1080 c         1418 g         1902 t
ORIGIN      
        1 ggatttgatg acatcgaatg gggcgaagac tttggttcac ctcatgaaac gttcattgcg
       61 aattcattcg aacagcctgt ctttatccta aactatccaa aagccatcaa gccattctac
      121 atgaaaccac atccaacaag agaagacgtg gtaatttgtg cagatatgat cgcaccagaa
      181 ggttacggtg aaattattgg cggaagcgaa cgggcaacag actatgattt cttgctagaa
      241 caaatccgta atcacggttt ggatgaaaaa gaatattcat ggtatcttga tctacgtaaa
      301 tacggttctg ttccgcactc tggttttggt ctaggccttg aacgaacagt cacttggatt
      361 gctggtatcg accatgtacg tgaagctatt ccattcccac gtttgttgaa ccgaatttat
      421 ccataagagg tgatgaaaaa gctgttctgc atcaagtaat tagaacagcc aagcaaaaac
      481 agctgttcat gtttttgaat tggcgggact tattaccgca gatgtaagct tttgaatacc
      541 gtttagtgga gaggttgtga aaaagctgtt tagtttcgag tattaagaaa aattttgtgg
      601 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
      661 accgtttaag gaagaggttg tgaaatcagt gccttgacct gagtattaga gaaacaaaac
      721 ggaaaaatag cttctcatat tttaccgttt ttttagctaa tatcgagggt cagctgattg
      781 aacaccgttt atatcaagag gttgtgcgcc tgccgttcag ctccgagtca caaggagtaa
      841 tttcaaaaaa cagtttttca tgtttgatga ctattgtgac ttgtgaccga ggagttggcc
      901 ggagagcaac gtttatttaa aaaaaagaac tgcgaactct cgcagttctt tttttaaaat
      961 acgggataaa ggaagtttcc agcagacaag agaagaaagc aaaagaaatt gctatttgat
     1021 tcacaaggtt ctataacgaa atcgcaatca atcaaacata tacaaagaag gttattcaaa
     1081 tggaaatctg atttgatgat gcttctgctg ttgaaataaa gcagcatcta gtacctgaaa
     1141 acaaactaat agtaacattc gaagatggag taggtccgta ttttcagcat gcaatgatcc
     1201 atatgtaagt ttaattcatc cttgaaatca tcggaccaga taaagttatg accaatctat
     1261 cccaagcaat ctagaagaga tattagtaaa agaacattta ctggaagtct tagatgagcc
     1321 tataaatata tgttctgatg gcgtcatcag tggtctgatt gacggaagct taggtgtgat
     1381 cgatttctct ggaccaaaca acgaaggaat caaggggaat ccggcacgtt aagaaaaaaa
     1441 gtataaaaaa ctagacactt gatgagcgtt cattcaattg attgaaggga ctaggctggt
     1501 gtcatttttt atacatatat tttctctttc gtcttgatag ggcttatggg ataatataag
     1561 gaagaaaatc atcgttttat tcaaaaagga gtttttgaca aatgaaaaaa cgtatattat
     1621 ttatcggtga cagcattaca gacgcaggaa gagaccgagc agaagcaact gatctaggta
     1681 aaggttatcc acttttgatt gccggaaaac taatctccca atatccaacg atgaaatttg
     1741 aatgcatgaa ccgtgggata ggaggagaca aaattgcaga tctgcaagta cgctggcaag
     1801 aagattgttt agctttacaa cctgactatg tatcgatttt gatcggaatc aatgatacct
     1861 ggcataatac tagcgatcag gctatattcg gaactgagaa atctgcgcag caatttgaat
     1921 ccgtttatcg aaaattacta aatgaacttc aaacagcgga aatctgcaac atcatcttga
     1981 tggagccgtt tgtacttcct ataaccgacg accgtcatgg ctggcgtaaa gacttagacc
     2041 ctaaaatcca aattgttcgt aaacttgcta aggaatatgg cgcattgctg gttcctttag
     2101 atggcatatt caatgcttta gggattgctt atggtttttc gaaatataca agagaagatg
     2161 gggtccaccc aacgattggc ggacatgaga tcattgcaca aagctggata gatgcaattg
     2221 ccgcagtcga tttttttagt ggagataaaa atgagcgaaa gaaaagtgag aaatgatgga
     2281 aatacggtgg gcagtaaaag aagatttacc tcaaatcaat cagctgatta ctgatcatcc
     2341 agaatcattc gtaaaagcaa aaacggcagc tgacctaaat gataagaata aactattgct
     2401 cgttgctgta acaaaagaaa aacggattat cggatttgcg caagcggacg tgcaacagtc
     2461 agaagaacgg gtggatcagc gaccatcagg aaaactaatc caacacacat tagtaacaaa
     2521 agaaaatacg gaagaaatcc aaaaaaaatt tgcagaatac ttgatgctgg aagctaaaaa
     2581 aagaagaata aaatcaattt ttgcccagtt ttaatcaaaa acagtaatag aaggagaaat
     2641 acaatgagtt gtttaggaaa tgtcatctgg tttatattcg gcggactaat cggtggtctt
     2701 agctggttag cagcggggat actttggtgt atcacgatca taggtatccc aatcggtatg
     2761 caatgtttta agatcgcctc cctttctttt gcgccttttg gcaaagaagt aatttatcac
     2821 cagtctggca tgtcattgat cgttaatatc atctggctga tcgtttcagg cctaccaatt
     2881 gctctcgcac atgtcgtcag cggcatcctt ctatgtatca cgatcatagg gatacctttt
     2941 gccaagcaat ctttcaaact tgctcgattg gcgttgatgc cattcggtgc agaaattgta
     3001 taaaaagaga ataataaagg ttagaaaacg catagagtca ggtgggatac atgtatttca
     3061 cctgactcta tgtgttttcc tgtataagtt ctcccatgtg acttttacgt cttttattta
     3121 tatgggatta ctgtttatgg aaagttgaaa taattttcat taaagaactc tttctatatt
     3181 tttgaaagcg tagtggagct tcgaatatct gggaaggaca ttttgcaggg attgaaaaaa
     3241 gatattgtcc tatcgaaaaa ttaatgaatt tagacaaatt gccagcaaca ggcttcaaac
     3301 ttagttgcta tcctgtcaag atcaagaagg caagtgcggg ttggataaga gcaggggcaa
     3361 tgatcgaaga gaaaaagaaa gaataagcca aaacatctgt atggaagaaa ttttccgatg
     3421 cagatgtttt tcattttttc tttcggatac cttgacatat gaataaccat tcatttataa
     3481 ttagttatga agagatgttc atatgttgag gcgaggaggt cattaaaatg aaaagttaca
     3541 gattagaagg tttagattgt gcgaactgtg caatgaaaat cgaaaaagga gttcagaaga
     3601 tcaatggagt caaggaagca acggtaaact tcacttctgg aaaactaacg attgacgcag
     3661 aggaagatca tttggctacg attgaacaag aaacaaaaaa agtagtgaag gaattagaac
     3721 cagatgtcaa agtaacagaa atagataagg aaaaagtatc tgaacatgga aatgaaaaag
     3781 agagaaatac gctgttccgg attttatttt cccttgcagg tatcgcttta ttgctattat
     3841 ttgattttaa tgaaccaatt cgcttgatag ggtatttatt gatttatctg ctgataggat
     3901 atgatgtagt gaagaaggca gtgatgaata tcgtcaaagg aaaaatcttt gatgagaatt
     3961 ttcttatgtc tgttgctacg attggcgcta tgttgatcgg tgaatatcca gaagccgtag
     4021 cagtgatgct attttatcaa atcggcgagt atttccaagg attggcagtc agtcattcaa
     4081 gaaaatcgat tcgcgaattg atggcgatcc gtccagaaac agctcacgtg cagacgacgg
     4141 aaggattgat gaccgtgaac cctgaagacg tgcagatcgg tcagtttgta cttgtgaagc
     4201 ctggtgaacg tgtaccgtta gatggaacga tcatcgaagg agaatcatta gtcgatacgt
     4261 cagcacttac aggagaatca gtccctaaat ctgtatatgt tggtgaaaca gtattaagcg
     4321 gatttatcaa taaaaataaa ccatttttag ttcaagtaga aaaatcttat gaaaattcta
     4381 ctatcagcaa attattagaa ttagtagaaa atgccagcag taaaaaagca ccagctgaaa
     4441 atttcattac taaattcgcg cgctattaca caccagtcgt tgtaggattg gccgttttat
     4501 tagctgtttt acctccactt gttgtttctg gagcggcgtt ttctgaatgg atctatcgag
     4561 cattgacatt tttagtgatc tcatgtccat gtgcattagt gatttccgtg ccacttagtt
     4621 tctttggcgg tatcggaggc gcaagtaaaa tcggtgtatt agtaaaaggt tcaaattact
     4681 tggagttgct tgcgcaaacc gagacagtgg tattcgataa aaccggcaca ttaacgaaag
     4741 gtgacttttc gatccagacg atcgatacaa aaatcgatcc aaagaaattc atgcaatata
     4801 ttgcgagtgc agaacagttt tctacacatc cgattgctca atctgtttta gaaggctacg
     4861 caggtccgct tttaccaaca actaatatcc aggaatttgc aggtgaaggg atcttagctg
     4921 aagttgatgg caaacaagta ttagcaggta atcacaaatt gatggaaaga tttgaaatct
     4981 cttttccttt aagtcaagaa atcggtacat tgctttatct agcgatcgat caaagttaca
     5041 gtggttatct tgttattgca gataccttga aagaagatgc cgtagacgca ttggttcaat
     5101 taaaacaggc tggtgtgaag aacacagtca tgttgacggg cgactcgaaa aaaattgctg
     5161 atcatatcgg gaagcaagta ggcgtggaca agatatatag cgaattgctt cctgaagata
     5221 aagtgcagcg gttagaggaa atcttacaaa gaaacaataa aaagaccgca tttgtcggag
     5281 atggaatcaa cgatgcaccg gtgctagcaa gagcagatgt aggaatcgca atgggtggat
     5341 tgggaagcga tgctgcaatt gaagcagcag atgtagtcat catgaatgat caaccatcga
     5401 agattgcaga agcaatccat ttagcaaaga aaacattgaa aatcgtcaaa caaaacattg
     5461 tctttgctat tgggataaaa attttggtac ttgccttagg tgcttttggt tttgcatcaa
     5521 tgggagatgc agtatttgca gatgttgggg tgacagtatt agctgttttg aatgccatgc
     5581 gaagcttgca tgtaaaaaaa gaggacgaat gaacgtcctc ttttttttat atccttttct
     5641 taatgaaaaa tgtaaagaaa atagatagga ttataaaata actaatgttt ttaattttgc
     5701 agaaaattca aaatagcttg aaattcaata aggtcaatga aataaaagaa aataacttat
     5761 ttttttgatg gaaaaaatat gaaaaaacga tgtgtattaa ataaaagaat acacacataa
     5821 aataacactg tcttgtaagt ttgttaaaaa ttgaatatga ttacatctgg ataacaaatt
     5881 tttatcataa aggagagaac gaatgaacaa caaacaatta cgctggttta ctgttgcgtt
     5941 aattgcattt aacatggttt ggggcatggg gaatgtagtt aataactatg ctcagcaagg
     6001 agtatcagtt gtcacctctt ggctattgat tttggccatt tattttattc catacgcatt
     6061 gatcgttgga caattaggat cagccttcaa ggatagtaaa ggaggcgtca gttcatgggt
     6121 cgaaaatacg acatctaata aacgactggc atactatgcc gcttggactt actgggtcgt
     6181 ccatattcct tatttggcac aaaagcctca agcaattctg attgctggtg gttgggcagt
     6241 ccaaggaaat ggaaatatcg taaatacgat gtcagtacaa atggtagcag ttatttcact
     6301 tattatattt cttgcctttt tatatttatc aacaaaagga ctttctactt tgaaagtgat
     6361 tggcggactg gccggaacag cgatgttcgt catgtcgatc ttatttattc ttttagctgt
     6421 aacggcaccg ttcatcaatc caacgatgga atttgccaca ccacatatga atcaagtgaa
     6481 aacatatatt ccaacatttg atttcagtta tttcacaacc gtttcgatgc tagtatttgc
     6541 agttggaggg gcggaaaaaa tatcccctta tgtcaa
//
LOCUS       DAYLZO010000134         6534 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.137, whole genome shotgun sequence.
ACCESSION   DAYLZO010000134 DAYLZO010000000
VERSION     DAYLZO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.137"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..85
                     /locus_tag="ACGVJG_002625"
     CDS             <1..85
                     /locus_tag="ACGVJG_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HFJ6558534.1"
                     /translation="VITVGTARRELEKDVLEFLEKLPWNEE"
     gene            88..2445
                     /gene="rnr"
                     /locus_tag="ACGVJG_002626"
     CDS             88..2445
                     /gene="rnr"
                     /locus_tag="ACGVJG_002626"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFJ6558535.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            2467..2931
                     /gene="smpB"
                     /locus_tag="ACGVJG_002627"
     CDS             2467..2931
                     /gene="smpB"
                     /locus_tag="ACGVJG_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFJ6558536.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            3233..3952
                     /gene="atpB"
                     /locus_tag="ACGVJG_002628"
     CDS             3233..3952
                     /gene="atpB"
                     /locus_tag="ACGVJG_002628"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFJ6558537.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            4005..4220
                     /gene="atpE"
                     /locus_tag="ACGVJG_002629"
     CDS             4005..4220
                     /gene="atpE"
                     /locus_tag="ACGVJG_002629"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFJ6558538.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            4311..4835
                     /gene="atpF"
                     /locus_tag="ACGVJG_002630"
     CDS             4311..4835
                     /gene="atpF"
                     /locus_tag="ACGVJG_002630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFJ6558539.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            4822..5364
                     /locus_tag="ACGVJG_002631"
     CDS             4822..5364
                     /locus_tag="ACGVJG_002631"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFJ6558540.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            5387..>6534
                     /gene="atpA"
                     /locus_tag="ACGVJG_002632"
     CDS             5387..>6534
                     /gene="atpA"
                     /locus_tag="ACGVJG_002632"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFJ6558541.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLR"
BASE COUNT         2205 a         1132 c         1425 g         1772 t
ORIGIN      
        1 tgtgattaca gttggtacag caagacgcga attagaaaaa gacgtcttgg aatttttaga
       61 aaaattacct tggaatgagg aataaatatg acaaaagaaa cgataaaaga gaaaattctc
      121 tttttcatgg aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc tgaaggttta
      181 gggttccaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
      241 gaacagagta tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa accaatcctg
      301 atcgaaggaa catttcgagc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
      361 gaagatgaca tctatatccc aaaagaagcg actggttttg caatggatgg agacaccgta
      421 gcaatcgata tcatgaagcg agcaaataca gatatggatc gcggagcaga ggggaaagta
      481 gtcgagattc gagaccgggc gactactcag ttagcagggg aattcgttgc ttattcagaa
      541 gaagaaatac aagaaacaga tctttacggc gttgttatcc cgaaagatag aaagctcaac
      601 caatttaaag tatatgcagc agcagaggga gttcgtccag ttgacggaag tattgtgctg
      661 atcgaactga cccattaccc tgaaaaaggg tatgcgacta gtttagaagg gataatcaag
      721 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcaattgt cgtggctaat
      781 ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt tccagatcaa
      841 atcagcgaaa aggatataac tggacgtaga gatttaagaa atcagctcat tgttacgatt
      901 gatggagaag atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt agataacggt
      961 aattattttc ttggagtaca cattgcagat gtttcttact atgtgacaca aggcagtcaa
     1021 ttggacagag aagcatatga aagagggaca agtgtatatt tgactgatcg agtaattccg
     1081 atgatcccgc aacgtttatc gaatggtatc tgttcactga atccccatgt tccaaggctg
     1141 accatgagtt gcgaaatgga gattgatcca aatggacaag tagtctctca tgatattttc
     1201 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     1261 gatcaagacg aagaaacgat gcaacgctat gctcagctcg tacctatgtt ccaagaaatg
     1321 ggtgaattgc accgaatcct tgaaacgatg cgaatcaaac gtggcgcgat ttcttttgag
     1381 gatcgcgaag cgaaaatcct tgttgatgct gaaggacacc caaatgacat ccagctaaga
     1441 agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa tgaaacggta
     1501 gctgaacatt ataataaatt aaatctgccg tttatttacc gaatccatga acagccaaaa
     1561 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     1621 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     1681 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     1741 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     1801 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     1861 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     1921 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     1981 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     2041 ggcctgttta tcgaactgcc gaatacagta gagggattga tccatatcaa tgaattgaaa
     2101 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     2161 acttttaaaa tcggacagaa agtgcgagta aaagtagtca aagctgatcc agatgaacga
     2221 gcagtagatt ttgaacttgt ttccgcagaa gaggttacag cgttggaacg gccaaaaaca
     2281 cgaaatggaa aatcaggaaa acgcgaagga aaacaagcag gaaaacgccc agaaagaaat
     2341 ggcaagaaga acaaacgata tagcgatagt aagcaaacgt tcggtaagaa aaaagggaag
     2401 aagccatttt acaaaggtgt taaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     2461 acagctatgc caaaaggcga gggaaaatta attgcacaaa acaagaaagc tcgccatgat
     2521 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaaccga gatcaagtcg
     2581 atacgaaaca gccggatcaa tctaaaagat ggatttattc gcgtccgcaa cggagaagct
     2641 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     2701 ttgcgcacga gaaagttatt attacacaaa aaacaaatca tccggcttga aaatgaattg
     2761 aaaaatactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     2821 gtattgattg gtctggcgaa agggaaaaaa tcttatgata aacgggaaga tttgaaacga
     2881 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     2941 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     3001 tctgataaaa catgattatc ataggttttt tcttagatca ggagcaccgt gtataaagaa
     3061 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     3121 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     3181 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     3241 acgctcgcta acgttccaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     3301 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     3361 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     3421 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     3481 tttatttgtt tttgttgcaa acaacatcgg tttagtaaca aaaatcgttt tacctaacga
     3541 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     3601 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     3661 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     3721 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     3781 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     3841 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     3901 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     3961 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     4021 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     4081 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     4141 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
     4201 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
     4261 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
     4321 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
     4381 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
     4441 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
     4501 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
     4561 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
     4621 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
     4681 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
     4741 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
     4801 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
     4861 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
     4921 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
     4981 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatggg
     5041 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
     5101 atgatcgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
     5161 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
     5221 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
     5281 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
     5341 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
     5401 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
     5461 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
     5521 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
     5581 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
     5641 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
     5701 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
     5761 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
     5821 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
     5881 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgatcgata cgatcatcaa
     5941 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
     6001 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
     6061 tgccggtgcg tctcaaccag caccattgct ttatatcgca ccatatgctg gtactgcaat
     6121 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
     6181 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
     6241 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
     6301 tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac aagctggaga
     6361 tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa tcttcttaga
     6421 aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat ctgtatctcg
     6481 tgtaggaggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacg
//
LOCUS       DAYLZO010000135         6516 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.138, whole genome shotgun sequence.
ACCESSION   DAYLZO010000135 DAYLZO010000000
VERSION     DAYLZO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.138"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1050
                     /gene="murA"
                     /locus_tag="ACGVJG_002633"
     CDS             <1..1050
                     /gene="murA"
                     /locus_tag="ACGVJG_002633"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFJ6558542.1"
                     /translation="EAPYEYVSQMRASIVVMGPLLARNGHAKVAMPGGCAIGKRPIDL
                     HLKGFQALGAKIIQKNGYIEAIADELIGNTIYLDFPSVGATQNIMMAAVKAKGTTIIE
                     NVAREPEIVDLANILNKMGAQVYGAGTETMRIEGVDHLHAVNHSIVQDRIEAGTFMVA
                     AAMTQGNVLIADAISEHNRPLISKLIEMGAEIIEEEGGVRVIGPKHILPTDVKTMPHP
                     GFPTDMQAQMTAIQLVAEGTSVVTETVFENRFQHLEEMRRMNAHVKIDGNVAIMDGNH
                     ELQGAEVYATDLRAAAALVLAGLKANGITRVRNLNYLDRGYYNFHIKLQQLGADVERV
                     DMDQTSAEKTAQTIA"
     gene            1063..1239
                     /locus_tag="ACGVJG_002634"
     CDS             1063..1239
                     /locus_tag="ACGVJG_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFJ6558543.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1374..1670
                     /gene="yidD"
                     /locus_tag="ACGVJG_002635"
     CDS             1374..1670
                     /gene="yidD"
                     /locus_tag="ACGVJG_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFJ6558544.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            1734..2201
                     /locus_tag="ACGVJG_002636"
     CDS             1734..2201
                     /locus_tag="ACGVJG_002636"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6558545.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2286..3170
                     /locus_tag="ACGVJG_002637"
     CDS             2286..3170
                     /locus_tag="ACGVJG_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFJ6558546.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            3284..3520
                     /locus_tag="ACGVJG_002638"
     CDS             3284..3520
                     /locus_tag="ACGVJG_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFJ6558547.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            3608..3688
                     /locus_tag="ACGVJG_002639"
     tRNA            3608..3688
                     /locus_tag="ACGVJG_002639"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3642..3644,aa:Tyr,seq:gta)
     gene            3695..3766
                     /locus_tag="ACGVJG_002640"
     tRNA            3695..3766
                     /locus_tag="ACGVJG_002640"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3727..3729,aa:Gln,seq:ttg)
     gene            3892..4062
                     /locus_tag="ACGVJG_002641"
     CDS             3892..4062
                     /locus_tag="ACGVJG_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558548.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            4282..4989
                     /locus_tag="ACGVJG_002642"
     CDS             4282..4989
                     /locus_tag="ACGVJG_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6558549.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKSGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5048..5692)
                     /locus_tag="ACGVJG_002643"
     CDS             complement(5048..5692)
                     /locus_tag="ACGVJG_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFJ6558550.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            5819..6106
                     /locus_tag="ACGVJG_002644"
     CDS             5819..6106
                     /locus_tag="ACGVJG_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558551.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6214..>6516
                     /locus_tag="ACGVJG_002645"
     CDS             6214..>6516
                     /locus_tag="ACGVJG_002645"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFJ6558552.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAI"
BASE COUNT         2168 a         1065 c         1428 g         1855 t
ORIGIN      
        1 gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta
       61 cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga
      121 ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac
      181 ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct
      241 agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc
      301 attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg
      361 ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta
      421 catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct
      481 gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca
      541 ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt
      601 gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc
      661 cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgtg
      721 gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac
      781 gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt
      841 gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa
      901 gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc
      961 catatcaaac tacagcaatt aggtgcagac gtcgaacgtg tagacatgga tcaaacttct
     1021 gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat
     1081 atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg
     1141 gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag
     1201 ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt
     1261 ctagtcgata tgaagtgagc catgaagcag aacatgataa aagaaaacag aagggaacgt
     1321 tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga
     1381 agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta
     1441 gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg
     1501 cgctaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag
     1561 gcggtatcga ttatgtacca ttgacattcc gattaacgcg aaataagaaa gataaaaaag
     1621 aaccagatta taaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag
     1681 gaaatttatt gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg
     1741 aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag
     1801 aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa
     1861 caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag
     1921 taaccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga
     1981 agctttcaca gctagcttca aaccaaactc ggatcattat acgtactggc actactagta
     2041 gcgctgcgtt acacctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact
     2101 attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag
     2161 tgattttagc aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa
     2221 aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata
     2281 tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg
     2341 atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga
     2401 aaacgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg
     2461 gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga
     2521 ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt caactcccag
     2581 taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc
     2641 gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca
     2701 cgaaaggaca aataaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct
     2761 tagatggcga taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt
     2821 gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag
     2881 aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg
     2941 gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt
     3001 ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat
     3061 tcgatctgca tgatttcgaa ccgcagattt tattatgtga gtacagaaac gatgctaatc
     3121 ttataggtgc agcagcaaac tatctcgctg tacgtcaagg agagaattaa gagctttaga
     3181 gaaggtgcag cttgcataaa agaagagaat gaaatgaata ggagattttt acctaaggaa
     3241 tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa
     3301 taaagatatt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga
     3361 agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa
     3421 agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc
     3481 gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt
     3541 caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa
     3601 acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg
     3661 ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa
     3721 gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt
     3781 aagctctcgt tagttcgaga gctctttttt tttgctttgc taccttgttc cttgaagatt
     3841 ctgctgaatt aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg
     3901 aaagaaaaag tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac
     3961 aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc
     4021 ccaacgaatc atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat
     4081 aagtcggcat tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa
     4141 aaaattgttt ctgacaatag gagtagggag aacgatgtgt gtctaaatcc tctgttgatg
     4201 gatagaaacc tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa
     4261 acgaaaggac gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac
     4321 caattaaaaa atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa
     4381 agagaattag cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac
     4441 ttggcagatg aaggaatctt agaaagaaag agcggttcgg gtacgtatgt tgcgcgagaa
     4501 aaagtacaag aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat
     4561 cgagtaccgt ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg
     4621 gaaaaactaa acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca
     4681 gatgatgtcc ctatttgttt tgaagttgcc agtgttccac aaaagctgat cgatggctac
     4741 agcaaagcag agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag
     4801 atcggtccag cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat
     4861 ttagaggtaa aacgaagaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac
     4921 ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg
     4981 gaaaaataaa aaacaggtga tccctgattc cttattagaa ataaaagaga atcagaatca
     5041 cctgttttta tatatgatca gttatcagtt gctcgtgata agaaacagaa ccctcttcaa
     5101 attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtta gcactttttt
     5161 catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat
     5221 gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga
     5281 tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat
     5341 gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga
     5401 ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgcccg
     5461 ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga
     5521 tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaat
     5581 tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga
     5641 tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac
     5701 ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt caacactgta
     5761 acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat
     5821 gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc
     5881 tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc
     5941 caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg
     6001 tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata
     6061 tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta
     6121 gaaaacgttg acaaatttct ttctttttat ataataaagt aacagacgcg aagaaaacgt
     6181 ttgcgtagtt tgttttaaaa aggagagaaa aatatggcta aaaaaacaat catgcttgta
     6241 tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca
     6301 aaaggaatcg actcggatat tttcgctgtg tccgcttctg acgcagataa caatcttgca
     6361 aataaagatg tagatgtact gttattagga cctcaagttc gctttatgaa agctgatttt
     6421 gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca tcaatatggc tgattatggt
     6481 atgatgaatg gcgaaaaagt gttagatcaa gcaatc
//
LOCUS       DAYLZO010000136         6502 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.139, whole genome shotgun sequence.
ACCESSION   DAYLZO010000136 DAYLZO010000000
VERSION     DAYLZO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.139"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..539)
                     /locus_tag="ACGVJG_002646"
     CDS             complement(<1..539)
                     /locus_tag="ACGVJG_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFJ6558553.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFK"
     gene            complement(546..1457)
                     /locus_tag="ACGVJG_002647"
     CDS             complement(546..1457)
                     /locus_tag="ACGVJG_002647"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFJ6558554.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            1577..2434
                     /locus_tag="ACGVJG_002648"
     CDS             1577..2434
                     /locus_tag="ACGVJG_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFJ6558555.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(2621..2983)
                     /locus_tag="ACGVJG_002649"
     CDS             complement(2621..2983)
                     /locus_tag="ACGVJG_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFJ6558556.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(3172..3354)
                     /locus_tag="ACGVJG_002650"
     CDS             complement(3172..3354)
                     /locus_tag="ACGVJG_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558557.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            3329..4768
                     /locus_tag="ACGVJG_002651"
     CDS             3329..4768
                     /locus_tag="ACGVJG_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558558.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(4775..>6502)
                     /locus_tag="ACGVJG_002652"
     CDS             complement(4775..>6502)
                     /locus_tag="ACGVJG_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFJ6558559.1"
                     /translation="LVVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSM
                     TVICSDKTGTLTKNEMTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVA
                     LIRHTETANLPLKKIEAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSA
                     EQEAWSQTAAEFARKGQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAV
                     KAVKECQQAGISVKMITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVP
                     IVDVFARTTPEHKLRIVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSK
                     QAADMVLGDDNFHTIAKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLP
                     LSPVQILWVNMVTTITLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLI
                     MVPAYLLAMQFEGQSLQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSL
                     GILALLQGMVIFLPVGQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKK
                     EKTAREYSR"
BASE COUNT         1889 a         1374 c         1094 g         2145 t
ORIGIN      
        1 tctttgaaag ctgcagcttc gatgattgtt ggtgtttctt ccgtattgaa acctgtgtac
       61 aagttgccgt ctttatctgc tttatctgca gctactaaaa caacatttgg aatcaaatcg
      121 atgaataagc gtccataaag ttccaaataa gtatggatat cacctaattt cattgtgccg
      181 tcttcaatca tttgggcgac acgcaagctt tgtgcaccag cataagagaa atcgatcttg
      241 cttgcgattc ctttttcaaa aatattcaaa tgttctggtc gtgagatact agacatgatc
      301 aaatgcagat cattcacttt ttctgcatct actttttcaa gactttctga aagaaaactt
      361 gcttgttttt gattgtcgcc ttctaataca actttgtcgc cgggagcaat cagtttttcc
      421 attacttcta cgattttttc agtttctaca tacttgccat tgagcaagtt gctgattttt
      481 tccaatcgct gcgctttttt ttcgcgttta gaagcccagt tgcggtttgg tttttccata
      541 attagtcatc tccatttttg atatagaaac gtggcgtcat caataccatg ttggtctaga
      601 ttgatcgtac ggatcccttt atctacacca aattttgtat gaatcagctt tgtcaaagta
      661 ttgccggggg gaaattcaat cgtcagatcc attcctaatt cttttgccaa gtccatcatt
      721 tggttccact gcacagggaa agctaggttt tccaacaaat ctttacgaat ctcttctgcg
      781 tcctgagtgg ctcttccatc cgtatttttt atatacaaac atctagcatc agatagatgg
      841 acagactgca tatacggctt gaatttcttg accgtatcat tcatcaagag acagtgagat
      901 ggcacaggga ctttcagcag cttcgctgtt tgcgcttggt gccgtttggc ttctgccaac
      961 gtaacggata aaccttcgat actcccagat aacgtatgtt gcatcggaca attttcattt
     1021 gacagataaa ctggatggtc aggatcaaat gtttttgcta caatttcctc agcttcgctt
     1081 ctcgtcacac ctactactac gcccattgca taaccgcttg gataagcttc tttcattaag
     1141 actgcgcggt gatagactag ttccgctgca ttctcaaatc tcaaagatcg acaagcaaca
     1201 gctgcacaaa aagcaccaat cgaatgtcct gctgcaatat caggctgaat accgtttttt
     1261 tccatttcat ctaaataaaa tactgctttt gtcagtaatg ctagctggat aaaaaccgta
     1321 tcttgatagt tttcttcttt atcaactaac cgataccccg tcacattttc taaacgttcc
     1381 atgtacattt cagggatatc cgctaacatc cctaacttct gactgccttg tccaggaaat
     1441 aagaaacctg tactcatgag cctcgcctcc tcagcactta ctttttccac tgctatctta
     1501 gtcaatttct gctataataa aaaatgccaa aaaaagaaaa aaccataaca aaaaggtatg
     1561 gatgggatgg ataattatga aattgcttca tctgcagtat tttaaaaaag tattcgaaac
     1621 aaaaaatgtt acccaagcag ccaaagaatt atttatttct caacctgcct tgagccgtgc
     1681 gatcaaacat ttggaaaacg aattaggtgt ccctttgttt taccatagcg gcagaaacat
     1741 tgagacgacc gtttatgcag aagaatttta tccatatgca gtcaagacat tagacacatt
     1801 agaagaagga atcaaattat tgtcttcggt caatgagaag atcgttactt ccgtcatatt
     1861 gtatttagag gtagcctctg tttctattcc gaacttggtc cgaattttta tggaaaagca
     1921 tcctgatatc caattaacga tcatgcaaca tcatttacca gatgatatta ccgatcctgt
     1981 actgtacatc acttcggaga tgaagcctgg catgaccaat attcctatca tgaaagaacc
     2041 tgtttatgtc gccattccta agaaacatcc tcttgctaaa aaagaacatc tgcaattaga
     2101 agatatccaa cgcaccccca tcttgatgct gtcaaagagc aacgccttcc gtcatacact
     2161 ggatgcagca cttgaagcga aaagtatcga attgacgatc agttcgacga cagatgatcc
     2221 tgcgacatta cgttctattg tcaagcaagg attagctgtt agctttttcc caaaagtttc
     2281 ttggtcttac gataaaaatg atcctttcgt cttgcgggaa atcaaagatt tgcccttgac
     2341 acgaacaatt tatttatctt ctagcttcac agaagatcat cctttgatca aaatgattgc
     2401 aaaaacgatg cgggaatttt atttaggaaa ataaaaaaaa aagagctggg acaaaattct
     2461 tgtcgcagct cttttttcga acaaacggtg ttccagaagt aatttcttag aaaataagcc
     2521 gaaatttccc aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagcta
     2581 aacatttctg tcacaacctc ttctctattg tcctagacat tcattgcgtt gatttaaaac
     2641 ttccatcaat caaatctgct agttcatcta gcttttctat tttacgtaga tcaatagaga
     2701 tccaatgttc tttattcatg tgataggctg gaaaataccc ctcttttcct cgcaaaattc
     2761 cgccaagttc aggatcgacc ttcaaatcaa gaatattgat gatcctatgg tcacttgcat
     2821 catcatagat tttttcttct ggaacagaca tcaggacccc aaaccatttt ttgctttttt
     2881 gatgtctgaa tacgcaataa tcaggaaatt ttttaaaaat atattctgga gacaaattgt
     2941 atgtttcatt gatgtgatcg attagttctt ttttcgttag catctgcttc catctctttc
     3001 ttattgattc gctttattca aagataccac gtaagaatag aaaaatgaat acaagtagtt
     3061 gctaatcaaa tacttcgata tttttctttt ttatcaactc ttaaatgttc aatccgcctg
     3121 tgttggtctt gctaccatct actattaaaa tgtttattat tgtttgccca cctatttttt
     3181 gattttagtg gtatcatttt tcaagttcat gatgaaattc ttatcatcag taaatgctcc
     3241 agaaaaataa acaatgattg aaaaatcagt tgaaacttga ccaccgttta ttttctccgt
     3301 attcttagaa tagaagggag tctgattgat gtattcacta atgaaaaaaa tcattaccga
     3361 aaaagatatt cgacgtcatg tttctttagt agaacaattg ctgaatcata caaagataac
     3421 cgtaaatgaa ttagctgaaa tcatagggac aaccgaacga acgatttttt ctgaccttca
     3481 atctattcgc tctcagctgc ccgaaggctg ggacatcatc agcgatcaag caggaatcag
     3541 tttgcaaaac cagcaaaatt tattaacaaa tgacctttgg gagatttttt tcaaacaatc
     3601 agtcagtgtc gaactgctga aaaatctgct ttttacaaaa aaagttgctg tccctgattt
     3661 tctagccgac catgggcttt cttatgggac attgaaaaga catgtgacta aaatcaatca
     3721 gcgtttagca agttacgact tgcagatcga tttgacgaaa tatacagctt gtattcttgg
     3781 aaaagagcgt gcgattcgta ctttttatca tcgattgctg attcctttca ctcataacaa
     3841 ctattttttc gaggattatt cgatccatga atcccattat tttcaatttt taagaaacct
     3901 cagtcaaact gaactagcgg ttgaaacaga agagattttc ggaacatgtt ggttctttat
     3961 caatacgatc cgtataaaag cgaattgccg attagattct tctatccata ccaacagcac
     4021 actctcctcc ctgtatgact ctgcattgaa aaaattgtat ttaacggaag gtatttatct
     4081 taaagacact gagctttctt ttgcttcttt ctgctttctg gaaagttgga attacaacaa
     4141 taactatgga caagaaaccg ctcgctgcct gcatcattct ccatttttag aggtcctcga
     4201 aacgtttgta gaggaactag cttcagagct gtctcttgat caactaaaaa aaacatctct
     4261 gactgataac ttggctctcc tcatattaaa atatcatgaa tcgccgatat tgattgaagc
     4321 attggaccgt caataccatc gttttttaga aacatatgat cagcaatatg cacacttgta
     4381 tcaacagaaa gagaatttac tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa
     4441 ttactttctc caactcttgt ctctactgat ccaacaaacg attttttcta tcaaaccgaa
     4501 tttgttcaat gtctatttta ttttccaagg agaaccttca tggaaggcgt tcttgcagca
     4561 ggaattaaaa gattacttag gaaaacgtgt ctgcttctta cctatcgaac taaccgatct
     4621 ttccactatt tcttttgaaa aaacagatat cttgatttcc aatttccctc tagattctct
     4681 agatatccct attgtataca tttcttctat tccaacgaaa aatgagctca atcgtttgac
     4741 tgaactcact ttcaaaactt ttttatagta acggctagcg actgtattct ctagctgttt
     4801 tttctttttt gcgaaacagt ttgttcatga tccctttttc tatttctaca actaagaata
     4861 gaagaatcac atttgctcca atcaataact gttgtgtcat cgttaaactt actgtaccga
     4921 tcagctgctg gccgactggt agaaaaataa ccatcccttg caataaagca aggataccta
     4981 atgaaagaaa aagcgctttg ttttgcaaaa gaccttggtt caatgacggc ttggacaact
     5041 ctctgcaatt gatcatgtaa acagcttgag ctaagacgat attttgcaga agaatggttt
     5101 gctgtaagct ttgcccttca aactgcatag ccaataaata ggcaggaacc atgatcagta
     5161 gggagacata gatgatccga aacaaatgat aaccagatag gattccttgc tttggatctc
     5221 ttggtgggcg cttcatgatt tctggatctg gcgactcgaa ccctagtgca taagataaag
     5281 tgattgtcgt taccatattg acccataaaa tctgtactgg ggacaaaggt agcgggcgat
     5341 tcagcataag tgcccaaatc aagatcaatc cttgagctaa tgcagttggc aagaagaaat
     5401 tgatggtttt ttgcaaatta tccaaaatcc gccggccctc ttttacggct tttgcaatcg
     5461 tatggaaatt atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt
     5521 tgattcccat agcaattcct acatcggatc ttttcaaagc aggtgcatca tttacgccgt
     5581 ctccggtcat tccgacgatt tctccatttt tctgcaaagc tttaacaatt cgcagtttat
     5641 gttctggtgt cgttcgagca aagacatcga ctattggcac ttgctgaatc aactcttcat
     5701 cagacataag atccaaatcg atcccttcta aaactttggc agtatgtttt aagccaattt
     5761 gttccgcaat cgcttttgct gtgtctttgt gatcccctgt aatcattttc actgagatcc
     5821 ctgcttgttg gcattccttt acagctttga ctgcactttc ttttggcgga tcgatcaatc
     5881 cggcgatccc tgcaaaagtt aattgggtta aagtctcatg cgtgagttcc tgtttcgaat
     5941 ctactttttt ataagcaaat cctaaaacac gctgaccttt tctcgcaaat tctgcagctg
     6001 tctgactcca tgcttcttgt tctgcagaag aaagagttga tttagcaaat aatacttctg
     6061 gtgcaccttt gacgtaaatc accgctcctt tttcctccgc atgcatcgtt gccatatatt
     6121 tgtattcaga actgaacgga atctttgctt ctattttttt caaaggtaga ttggctgttt
     6181 ctgtgtgtcg aatcagcgca acttctgtcg gattgccact taaattttct gttttctgtt
     6241 cttcttttgt ttttaattcc tgacagtttt tcataatgga caacacgcag gcttcttctt
     6301 ttgcagctac atccacaact gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac
     6361 agatcactgt catagaacct aatgtttcta ccgaaggaag tcctttgatg atcgcctttt
     6421 cttttgccat ttcatgtact ccaacggata gaatcatggt caaaacagct ggcaatcctt
     6481 cgggtaccat tgacaccacc aa
//
LOCUS       DAYLZO010000137         6434 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.140, whole genome shotgun sequence.
ACCESSION   DAYLZO010000137 DAYLZO010000000
VERSION     DAYLZO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.140"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1682
                     /locus_tag="ACGVJG_002653"
     CDS             <1..1682
                     /locus_tag="ACGVJG_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HFJ6558560.1"
                     /translation="IHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTE
                     QQQMIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAM
                     KKLELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSV
                     SVVLMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYD
                     SIGTNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMS
                     FGDATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLL
                     EEAPGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAE
                     NPHNFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKER
                     NPEVTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERIS
                     DGQLDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            1711..2187
                     /gene="ybeY"
                     /locus_tag="ACGVJG_002654"
     CDS             1711..2187
                     /gene="ybeY"
                     /locus_tag="ACGVJG_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFJ6558561.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            2171..2566
                     /locus_tag="ACGVJG_002655"
     CDS             2171..2566
                     /locus_tag="ACGVJG_002655"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFJ6558562.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            2581..3480
                     /gene="era"
                     /locus_tag="ACGVJG_002656"
     CDS             2581..3480
                     /gene="era"
                     /locus_tag="ACGVJG_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFJ6558563.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            3623..4441
                     /gene="recO"
                     /locus_tag="ACGVJG_002657"
     CDS             3623..4441
                     /gene="recO"
                     /locus_tag="ACGVJG_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFJ6558564.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            4827..5744
                     /gene="glyQ"
                     /locus_tag="ACGVJG_002658"
     CDS             4827..5744
                     /gene="glyQ"
                     /locus_tag="ACGVJG_002658"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFJ6558565.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            5746..>6434
                     /locus_tag="ACGVJG_002659"
     CDS             5746..>6434
                     /locus_tag="ACGVJG_002659"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFJ6558566.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELA"
BASE COUNT         2131 a         1172 c         1405 g         1726 t
ORIGIN      
        1 aaattcacac agttagggat gaaagtttga agctcattga cgaacagatg agtaaacaag
       61 ttcgtgaaag cgaattggaa gcttttaaac aagaagcaga agatcagatt caatatttga
      121 atgtcacaac agagcagcaa caaatgattc gctacttagt ggatcaaggc atcgtagtta
      181 atgatgtatt gaatgagaaa aagacagaag aattaaaaca atctgcacga gaagctgtac
      241 aaccagtgat gatcttccaa ggagaaatta ttgttcgcga aggaaatcaa attgatgctt
      301 cagcgatgaa aaaattggaa ttactaggat tgacgaatca aacgacttct attttcccac
      361 tcattgccat ggttttagct gttttgcttc aaatcggtgt actgatttat aattcacttc
      421 aattccaaaa tgcaggagaa agaacaaaat acgtactttt ctacgtgacg gcaatgtcag
      481 taagcgtcgt actaatgaaa tttttccaac tcttccaaac agaacaagct gcttatattc
      541 cgctgtttta ccccgcggct tttgctccac ttgttttaag ctttttcttg aatcgaagag
      601 cagggatcat ggctggtatt tttcaagcag tatccgcact tttcattttt tatgattcga
      661 ttggcacgaa ctttctgaca gttattttga tgtcctatgt attttctggt ctcatggcaa
      721 cgatcgtcag acgcaaacgg atctcagaac aagggttatc cgctgctatg tgggtcatta
      781 tttttcctgt gatgatggat gtcattttga ttatctacca aggaatgagc tttggggatg
      841 caacgacttg gttaacgcta gtctgcggat tcacaggtgc actgttttca ttcttgctga
      901 cgataggtct gcacccctat attgaattga tggtcaatga tgatagtgtg atcgtattaa
      961 atgaactcag caatcccaat catcctttat tgaaacagtt gctggaagaa gcaccaggta
     1021 cgtatcacca tagcatgatg gtagccaatc taagtgctaa tgctgtagca gaaatcggtg
     1081 gacgttcttt actaactcga gtagcttgct attatcatga tatcggcaag atcaaacacg
     1141 ccagcttttt cgttgagaac ttgccttcag gagcggaaaa tccgcacaat ttcttattgc
     1201 cagaagacag taaacatatt atctttggac atgtgacaga tggtgccaaa atattggaag
     1261 attataatat gccgcaaatg gtgatcgata tttgtagaca gcatcatgga accacgctga
     1321 tgcgttattt ctacgttaag gcaaaggaac gcaacccgga agttacggaa gagcaattcc
     1381 gttatcctgg accaaaacct caaagccgtg aagcaggaat cgtcaatata gctgacagtt
     1441 gtgaagcggc agtacgggca atggaccatc caacgagcga aaaaatcgaa agttttgtcc
     1501 ataatcttat tgaagagcgt atttccgatg gacaattgga cgacagtgga ttgacgttaa
     1561 aagaaatccg taaagtagaa aaatcattga tcagcggttt aagcagtacc ttccattctc
     1621 ggatcaaata tcctaaaatg aaatcggaag ttgagaagat aaaagaagaa caagaagaat
     1681 agcaagaaga atagaaagaa gcgatgtata atggatatta ccttcatgga tgaaacaaac
     1741 caagtctcag aagagaaaat gaaagaaatc gacgacttgc tgcaatttgc agcatcatat
     1801 ttagagttgc cagaagaaac agaaatgtct gttactttca tggacaatgc agcaatccaa
     1861 gtaatcaatc gtgattaccg tggaaaagat gcaccgacag acgtcatcag ttttgccttg
     1921 gaagaagagg gcgaagacga aatcccagtg atttttgaag atggagagaa tccattgcct
     1981 cgtgtactgg gagacatcat gatctctact gaacgtgctg cagagcaagc agaagcttac
     2041 ggccattcgt atgatcggga acttggcttt ttagcggttc atggattctt gcatatcaac
     2101 gggtatgacc atatgacacc agaggatgag aaagaaatgt ttggtttaca gaaagagatc
     2161 ttagatgcct atggacttaa aagataaaaa cgtagagaaa aacaaacatt tcctgatgtc
     2221 cgtggaattt gccgtccaag gagtcaaaac tgtttttgag gaagaacgca acatgaggaa
     2281 acatgttgcg ttcggaatcc tcgctttgtt tatgggcgtg attttccaac ttgatcggat
     2341 agaatggtta tggctgcttt tagctgtttt tctcgtatgg atagtagaga tcatcaatac
     2401 tgtttttgaa aatgtcgtgg acatgtttac ggattttcat tttcatccga ttgggaaaaa
     2461 aatcaaagat atggcagctg gagcagtttt agtgaccgct tgttttaccg tcatcgttgg
     2521 gttgatttta tttgtcccta aaatatatca tttgttgttt aattaaaaag gagaacatat
     2581 atgccagaac ataaatcagg gtttgtcgca atcgtcggcc gcccaaatgt agggaaatcc
     2641 acattattga atcgaatcgt gggtcaaaaa attgccatca tgagtgacaa ggcacagaca
     2701 actagaaata aaatccaagg agtatacact acaccagaag ctcagttgat ttttattgat
     2761 acaccaggaa tccataaacc aaagcatcgt ttaggagatt ttatggtaga agcagcttac
     2821 agtgcgttga aagaagtgga cgcggtctta tttatgatta gtgctgatca aaaacgcggt
     2881 cggggagacg attttattat tgaacgtcta aaaaacgtgc attctcctgt ttatctgatt
     2941 atcaataaaa tcgacaaaat ccatccagac gagttgttgg gaatcatcga agattattca
     3001 acgcagatgc cttttgcaca agtgattcca atctctgcta cagaaggaaa caacgtcgaa
     3061 cgtttgatgg atgtattagt cagtgaaatg ccagagggcc cacaatattt tccagatgat
     3121 caagtgactg atcatcctga atacttcatc gtttctgaat tgatccgtga gaaagtcttg
     3181 tttttaacac gagacgaagt gcctcattct gtcgcagtcg ttgttgattc gatgaagcga
     3241 aacgagaatg ataaaatcca tatccaggct acgattattg tcgaaagaga tagccaaaaa
     3301 gggatcatta tcggcaaagg cggaaaaatg ctgaagcaag taggaacgaa agcaagaaaa
     3361 gatatcgaaa atcttttagg agacaaagtc tttctagaac tttgggtcaa agttcaaaaa
     3421 gactggcgag acaaaaaagt ctatctgcaa gacttcggct accgaaaaga cgaatactga
     3481 tattctgagc ttcttgtaaa ttaggtatag aaataaactg aataaaaatc cgcgtgttga
     3541 gaaaagctgc ggaagatcgc aacataaatg atctggatcg gcagaatctg cttttccaga
     3601 tttatttttt aggagggaga atatgagcca gcttgcagaa tcaaaaggct tgattttatc
     3661 tagccgtaat tacaaagaaa aagataaatt agtcaaaatc tttacagaat cttctggcaa
     3721 aatgatgttt tatatcaaag agatccatcg gcaaaatcag cccttagctc cggctttgct
     3781 cccttttact gaagcagttt atatcgggaa ttttcgaagt gaaggtttgt catttttaag
     3841 cagtgtcaaa gatgtccatg cgtttcgaca catccaagaa gacatctttt tgagcggcta
     3901 tggcacatat ttattgaatc tagtagatgc ggcaatcgaa gatcatcagt acgacccgca
     3961 tttgttccag tttacgcaac aagcactgca acggatggac caaggagacg atgcagaaat
     4021 catcacgaat atttttgaag tccaaatact gcaacgcttc ggaattgcac caatctggac
     4081 acactgcgtc gtatgcgggg aaacaaaagg aaaatttgac tattcttcca aatatggtgg
     4141 tgtgatatgc gaaagacatt ggtcgatgga cgatcaccgt tatcatgcag atcctcgagc
     4201 tgtttatttc gttcggatgt tttcagctat ttcttatgat aaaattagtg gaatccatct
     4261 gaaagaagaa accaaacaag caatccgtga actcatcgac cagctgtatg atgaatatgt
     4321 cggtatccat ttgaaaagca aaaagttcat caatcaaatg aagtcttggg cacacgtatt
     4381 aaagacgtca gagaaagaca tgtctgaaaa agaaaaagag acacctgaaa aagaagaata
     4441 gtaaataaaa catacaacaa aaaatcaaaa ccatttgaca aacacaggga gttccgtcta
     4501 ttattaacca tagattaaat agatcgttca gtgataggat ctattataga aacaagaaaa
     4561 ctgcaaaatg aatacgtaca ttgaccaaga tcagtaaaaa acacgtggtt tagcgagttc
     4621 gggagagtgg gagccggacc caaacgattt ttgaagaggc acttgcgagt acgataaatg
     4681 aagctgccga atctttattc ggaagtaggg tggaaccgcg aattattcgt ccctatgtct
     4741 gtaatcagac atagggattt tttgtcgttt tctttcctgt attcttattt gacagcaacg
     4801 ctttaaaatt gaaaggggca aaacagatga gtaaaaaatt gaccgtccaa gatatgattt
     4861 taacattaca aaaattttgg tcatcaaacg gctgcatgct gatgcaagct tatgacacag
     4921 aaaaaggagc aggtacgatg agcccatata cctttttacg ggcaatcgga ccagaaccat
     4981 ggaatgctgc ttacgtagag ccatctcgcc gaccagctga tggacgttat ggtgaaaacc
     5041 caaaccgttt gtatcagcat catcaattcc aagtggtgat gaaaccttca cctgagaata
     5101 ttcaagagct ttatttagaa agcttgcgcc ttttaggaat cgatccatta gaacacgaca
     5161 tccgttttgt tgaagacaac tgggagaacc catccatggg ctgtgccggt gtgggttggg
     5221 aagtatggct tgacggaatg gaaatcacac aattcacgta cttccagcaa gttggtggac
     5281 tgccttgtaa accagtaact tctgagatca cttatggatt agaacgtctt gcttcttata
     5341 tccaagaagt tgaaagtgtc tatgatttag aatgggctga tggcgtgaaa tatggcgaga
     5401 tcttcaaaca accagaatat gaacattcca aatattcttt tgaagtaagc gaccaagact
     5461 tgctactaag caactttgat cgttttgaaa aagaagctaa acgctgtatc gatgaaaatc
     5521 ttgttcatcc ggcgtatgat tatatcttga aatgtagcca tacattcaac ttattagacg
     5581 cccgcggtgc agtatccgta acagagcgtg ccggttatct ttctcgcatc cgcaacatgg
     5641 ctcgtgctgt agccaaaatc tttgtggcag aacgcgaaaa attaggattc cctttgttga
     5701 ataaaaacac agcaaatcca gaaaccgtga aggaggctga ataagatggc aaaagactta
     5761 ttacttgaaa tcgggctgga agaaatgcct gcccatgttg tcacaccaag catgaaacaa
     5821 ttagaagaaa aaacagcaaa atttttagat gaaaaccaat tgacttatga tacgatcgaa
     5881 acattttcca caccgcgccg tttagcaatc aaagtaacaa atattccaga acgacaagcg
     5941 gacagtgaag aagaagtcaa aggaccagcc aaaaaaattg ctttggacgc agatggaaac
     6001 tggacgaaag cagcggaagg atttgtccgc ggacaaggtc tgacgacaga cgctatcact
     6061 tttagagaat taaaaggtgt ggaatatgtc tatgtaacga aatacactca tggtaaggaa
     6121 gcaaaagaag tattgacagg cttagcaggg gtgatcaaaa gtttgacttt ccctgtaacg
     6181 atgcattggg cagaatacga ttttgaatat atccgcccta ttcactggat cgtggcattg
     6241 ttagatgacg aagtaatccc attcgaaatc ttggatgtca aaacaggcag agcctctcga
     6301 ggtcatcgtt tcttaggcga agatgtgaca tttgcgcatg caaaagaata tgaagaaaaa
     6361 ttgaatgaac aattcgtcat tgcgtctccg caaaaaagac aagaaatgat cgttacacaa
     6421 gcaaacgaat tagc
//
LOCUS       DAYLZO010000138         6387 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.141, whole genome shotgun sequence.
ACCESSION   DAYLZO010000138 DAYLZO010000000
VERSION     DAYLZO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.141"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            330..1175
                     /locus_tag="ACGVJG_002660"
     CDS             330..1175
                     /locus_tag="ACGVJG_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFJ6558567.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACGVJG_002661"
     CDS             1456..2676
                     /locus_tag="ACGVJG_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFJ6558568.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACGVJG_002662"
     CDS             2673..5393
                     /locus_tag="ACGVJG_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6558569.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACGVJG_002663"
     CDS             5377..6321
                     /locus_tag="ACGVJG_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFJ6558570.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2378 a          987 c         1232 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacgaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DAYLZO010000139         6286 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.142, whole genome shotgun sequence.
ACCESSION   DAYLZO010000139 DAYLZO010000000
VERSION     DAYLZO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.142"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            190..690
                     /locus_tag="ACGVJG_002664"
     CDS             190..690
                     /locus_tag="ACGVJG_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFJ6558571.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACGVJG_002665"
     CDS             787..2634
                     /locus_tag="ACGVJG_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFJ6558572.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACGVJG_002666"
     CDS             2637..3533
                     /locus_tag="ACGVJG_002666"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6558573.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACGVJG_002667"
     CDS             3583..3972
                     /locus_tag="ACGVJG_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFJ6558574.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6286
                     /locus_tag="ACGVJG_002668"
     CDS             3959..>6286
                     /locus_tag="ACGVJG_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6558575.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITSRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2119 a          933 c         1346 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccag
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaac
//
LOCUS       DAYLZO010000140         6173 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.145, whole genome shotgun sequence.
ACCESSION   DAYLZO010000140 DAYLZO010000000
VERSION     DAYLZO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.145"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            725..811
                     /locus_tag="ACGVJG_002669"
     CDS             725..811
                     /locus_tag="ACGVJG_002669"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFJ6558576.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACGVJG_002670"
     CDS             complement(932..1621)
                     /locus_tag="ACGVJG_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558577.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACGVJG_002671"
     CDS             complement(1628..1906)
                     /locus_tag="ACGVJG_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558578.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACGVJG_002672"
     CDS             complement(1866..2780)
                     /locus_tag="ACGVJG_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFJ6558579.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACGVJG_002673"
     CDS             complement(2762..3142)
                     /locus_tag="ACGVJG_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFJ6558580.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACGVJG_002674"
     CDS             complement(3592..3879)
                     /locus_tag="ACGVJG_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFJ6558581.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACGVJG_002675"
     CDS             complement(3899..4123)
                     /locus_tag="ACGVJG_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFJ6558582.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACGVJG_002676"
     CDS             complement(4578..4748)
                     /locus_tag="ACGVJG_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558583.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACGVJG_002677"
     CDS             complement(4763..5296)
                     /locus_tag="ACGVJG_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFJ6558584.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACGVJG_002678"
     CDS             complement(5317..>6173)
                     /locus_tag="ACGVJG_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFJ6558585.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYLZO010000141         6162 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.146, whole genome shotgun sequence.
ACCESSION   DAYLZO010000141 DAYLZO010000000
VERSION     DAYLZO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.146"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..616
                     /locus_tag="ACGVJG_002679"
     CDS             <1..616
                     /locus_tag="ACGVJG_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFJ6558586.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            613..1386
                     /locus_tag="ACGVJG_002680"
     CDS             613..1386
                     /locus_tag="ACGVJG_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFJ6558587.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1383..1781
                     /locus_tag="ACGVJG_002681"
     CDS             1383..1781
                     /locus_tag="ACGVJG_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFJ6558588.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1966..2637
                     /locus_tag="ACGVJG_002682"
     CDS             1966..2637
                     /locus_tag="ACGVJG_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558589.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2696..3136
                     /locus_tag="ACGVJG_002683"
     CDS             2696..3136
                     /locus_tag="ACGVJG_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558590.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3148..3816
                     /locus_tag="ACGVJG_002684"
     CDS             3148..3816
                     /locus_tag="ACGVJG_002684"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFJ6558591.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3880..4503
                     /locus_tag="ACGVJG_002685"
     CDS             3880..4503
                     /locus_tag="ACGVJG_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFJ6558592.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4559..4945
                     /locus_tag="ACGVJG_002686"
     CDS             4559..4945
                     /locus_tag="ACGVJG_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFJ6558593.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5158..>6162
                     /locus_tag="ACGVJG_002687"
     CDS             5158..>6162
                     /locus_tag="ACGVJG_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFJ6558594.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     A"
BASE COUNT         1986 a          998 c         1355 g         1823 t
ORIGIN      
        1 gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg gtgcggcaat
       61 catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga tggaagtcgg
      121 agatctagta gggaaatata tgatcaaaaa gaaaagtttg accgttttgg tcagtttggg
      181 atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt tgggacaaca
      241 agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga tcgtcagcgt
      301 cggcactgga gtatttctag cctttgcgct gcttcgggtc gtattcaagc tatcctatta
      361 tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca ccagttccga
      421 atttatgccg attgcttttg attcgggggg agtgactact ggtccagtaa ctgttccatt
      481 tatcttggcc cttgccggag ggatgactag tatgatcaaa caaaagaaaa atgaaaatga
      541 cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga tgattttggg
      601 ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat cgttaccatg
      661 gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa tatcattagt
      721 tttcgtatgc gcaaaaagcg tttctttgac attatgaaag gtttcttgat aacgactatt
      781 gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg acagcattta
      841 ggcagtgcta ttgcgggatt gccttacaat tatatactgg ttccccttgg ctttttaatg
      901 gggttcttag tagggtttgc tgagccggcg attcatgtaa tgatcaaaca ggttgaagaa
      961 ttgagcgagg ggagaatccg atcgaaaatc atgctgatgg taatttcaat tgggatcggc
     1021 gcagcagtag gattgtctat gtggcgtttg ctcgtcggat tctctcttta ttatttcttg
     1081 atcccaggat acgggctagc atttatttta ggacgaaaag tggacaagat ctttttagca
     1141 atggcttttg ataacggtgg agtagcaacg ggcccgatgt gttcgacatt tattttatca
     1201 atgagcgtgg caatcgctgc acaaattgaa ggaagagatc cgatcattga cgggtttggt
     1261 gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg atatatttat
     1321 aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt gggggaaagc
     1381 aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt gacggaggaa
     1441 tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga acgatcttac
     1501 atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac atttcgccag
     1561 aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta atggatgcga
     1621 ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt attgatgtag
     1681 acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga atgcaagaac
     1741 ttttagatga aaataacgat agagaaaagt ctgaatcata gacaagcgtt tgaagaggaa
     1801 ataaacacaa acaagaaggc ggatgagcgt tatttccgct tttttgtttg tatttttctc
     1861 gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc agactttctt
     1921 ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa ttttttagtt
     1981 gttttaaaag atactctgat tgagcgattt acatttctat atgttcaatt gcaggaaaat
     2041 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
     2101 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
     2161 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
     2221 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
     2281 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
     2341 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
     2401 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
     2461 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
     2521 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
     2581 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
     2641 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
     2701 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
     2761 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
     2821 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
     2881 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
     2941 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     3001 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     3061 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     3121 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     3181 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     3241 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     3301 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     3361 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     3421 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     3481 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     3541 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     3601 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     3661 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     3721 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     3781 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     3841 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     3901 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     3961 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     4021 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     4081 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     4141 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     4201 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     4261 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     4321 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     4381 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     4441 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     4501 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     4561 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     4621 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     4681 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     4741 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     4801 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     4861 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     4921 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     4981 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     5041 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     5101 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     5161 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     5221 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     5281 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     5341 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     5401 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     5461 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     5521 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     5581 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     5641 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     5701 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     5761 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     5821 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     5881 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     5941 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     6001 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     6061 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     6121 aaagctttag gagtagatga tgcaacagct aatttctcag ca
//
LOCUS       DAYLZO010000142         6021 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.147, whole genome shotgun sequence.
ACCESSION   DAYLZO010000142 DAYLZO010000000
VERSION     DAYLZO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.147"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(60..362)
                     /locus_tag="ACGVJG_002688"
     CDS             complement(60..362)
                     /locus_tag="ACGVJG_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFJ6558595.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(582..948)
                     /gene="ssrA"
                     /locus_tag="ACGVJG_002689"
     tmRNA           complement(582..948)
                     /gene="ssrA"
                     /locus_tag="ACGVJG_002689"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1119..1892)
                     /locus_tag="ACGVJG_002690"
     CDS             complement(1119..1892)
                     /locus_tag="ACGVJG_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFJ6558596.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2036..2386
                     /locus_tag="ACGVJG_002691"
     CDS             2036..2386
                     /locus_tag="ACGVJG_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFJ6558597.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2367..3083
                     /locus_tag="ACGVJG_002692"
     CDS             2367..3083
                     /locus_tag="ACGVJG_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFJ6558598.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3083..4531
                     /locus_tag="ACGVJG_002693"
     CDS             3083..4531
                     /locus_tag="ACGVJG_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6558599.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4602..5111)
                     /locus_tag="ACGVJG_002694"
     CDS             complement(4602..5111)
                     /locus_tag="ACGVJG_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558600.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5101..5826)
                     /locus_tag="ACGVJG_002695"
     CDS             complement(5101..5826)
                     /locus_tag="ACGVJG_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558601.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2046 a         1050 c         1170 g         1755 t
ORIGIN      
        1 cccttttata agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt
       61 taatctttat acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga
      121 tcgctccgtg cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc
      181 attctggttt ttgttgccgg aattttaaag tagttcttat tagagtaata gagattgatt
      241 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
      301 ttgacaaatc tattcgaagg atcaaattta aaccataatt gtgtttttcc ttccatcaac
      361 ataatgttca cctcaaaaag agtatacgaa ctaatgttct ttttgtaaag taggaatatc
      421 attgataata tggatggaac atatatatta aaaagtgttc tttttgaatg ctattttgaa
      481 tgccattttc attcatttaa cgaaaatctg cgaaaataaa aaatgctcaa acccttataa
      541 aataaggatt taagcatcta gcgaaatctg ctgaaatctc acaatggaga cggcgggagt
      601 cgaacccgcg tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa
      661 ggattcactt tacagcttgc cgagtgacag gcattctgta tcgctagtct gatgttctct
      721 ttttcaactt acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga
      781 gcacatgggc gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga
      841 gttgttttcg tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac
      901 gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa
      961 gggttacgtg tatttttcat acaaaattag tatagcatat attgggataa attcaataaa
     1021 aaagcgaatc atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc
     1081 gattttctta agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc
     1141 aatgcgtgga caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt
     1201 aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa
     1261 tcattcggac catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc
     1321 agacgtttga cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga
     1381 ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga
     1441 gataagatca gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga
     1501 acatctgaat gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag
     1561 attttctgat cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt
     1621 ttttgacatt cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg
     1681 aaagaaccat tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt
     1741 tctgcttctg tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc
     1801 atagcttttg ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga
     1861 tcaaagaaag tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa
     1921 gtttatctta acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat
     1981 tggttcagtc aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat
     2041 ttttttcagt attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc
     2101 aaaaggaaat ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca
     2161 ttggcggctg gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc
     2221 attgatcggc tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct
     2281 tctgctagta tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata
     2341 tgacctggaa actggaggga acgataatga atattttaat gattgaagat aatcaatctg
     2401 tttctgaaat gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg
     2461 atggaaaaga agggctcgat gcctttttag aaaacccaca acattgggat atgattacac
     2521 tagacctgaa tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt
     2581 caagtactgt gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg
     2641 gcttagagat gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg
     2701 ctcgaatgaa agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagtag
     2761 tttcagatga tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag
     2821 aagcgtatct ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt
     2881 ttactttagc aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct
     2941 gggattatca gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac
     3001 aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat
     3061 tcgatgattc tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttctgcgg
     3121 aatggtcgcg ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac
     3181 gattgaagaa aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac
     3241 gcaaacctat gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt
     3301 gctgctgaca gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa
     3361 cgaacgtgtc atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca
     3421 atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg
     3481 acaagcacag gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg
     3541 agcacttgta gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact
     3601 gaacctgttc aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt
     3661 tgcagcattc caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa
     3721 tggtaatttt gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga
     3781 tgatttcaat aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga
     3841 aaatcgtcga agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac
     3901 aatcaacgga cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa
     3961 cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt
     4021 ggattatgaa aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac
     4081 agaaacattg aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac
     4141 attgacattg aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca
     4201 aatcatggtc aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat
     4261 tactttagaa aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat
     4321 gtctgaacaa caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa
     4381 aaatacaaaa tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt
     4441 gcataaagga aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg
     4501 tttgcctgac gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc
     4561 gatcttctat accgcttgtt ttttactata aaatgattga tttagcccgc gggctctaaa
     4621 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4681 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4741 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4801 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4861 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4921 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4981 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     5041 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     5101 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     5161 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     5221 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     5281 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     5341 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     5401 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     5461 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     5521 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5581 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5641 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5701 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5761 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5821 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5881 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5941 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     6001 aggagcagcg acaataattt t
//
LOCUS       DAYLZO010000143         5967 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.148, whole genome shotgun sequence.
ACCESSION   DAYLZO010000143 DAYLZO010000000
VERSION     DAYLZO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.148"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..912)
                     /locus_tag="ACGVJG_002696"
     CDS             complement(<1..912)
                     /locus_tag="ACGVJG_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFJ6558602.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKT"
     gene            complement(914..2353)
                     /locus_tag="ACGVJG_002697"
     CDS             complement(914..2353)
                     /locus_tag="ACGVJG_002697"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFJ6558603.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2538..4484
                     /locus_tag="ACGVJG_002698"
     CDS             2538..4484
                     /locus_tag="ACGVJG_002698"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFJ6558604.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4748..5620
                     /gene="scrK"
                     /locus_tag="ACGVJG_002699"
     CDS             4748..5620
                     /gene="scrK"
                     /locus_tag="ACGVJG_002699"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFJ6558605.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1893 a         1020 c         1071 g         1983 t
ORIGIN      
        1 tgtcttttcg cctttaattt ttttcagaag aatatcaaca ataagacttg caatttcatc
       61 aattggttgt cttattgttg tcaattgtgg gaaaaatttc tcaataaaag aggtaccatc
      121 gtatccaata atttttaaat cttctgggat attaagttta agttgatgag caattttcat
      181 agtgagaatg gctgttaaat catcagaaac aaaaatacca tctggtttgt gatttataat
      241 agttgacttt atttccattt cacgacggat agtagacaga ttattaggaa ttttgaaaat
      301 ttttccattt ggattttgaa aagaaaaacc tagtgcacgt aattctgtgg gagaatccgt
      361 gttatcattt cctgtaatca taatggtatt ttgacaacca tttttttgta aagtcttagc
      421 tgcaagtttt cccccttcaa agttatctga agaaacaatg gaaacattag gagctaaatt
      481 tctatcaaaa gcaacaatag gtgctctcac acgctcataa tcagcaattc caagattatg
      541 gcttgatgag attatgccat caacttggtt ggcttcaagc atttcaatgt attctctctc
      601 tttagcagga tcattttgac tattgcaaat aatcgtttta tatccatgtt taaataactc
      661 tatttcgaga tactcaatga gttcagagta aaagatatta ctaatattag gaaaaataag
      721 accaataagc tgaggtgact ttccttgaag tccacgtgct aaattatttg gtttataacc
      781 aagagttttc atagcttcat gaactttttg ttttgttttt tctgataaat atcccttatt
      841 attaatgacg cgtgaaacgg ttgttgggct aactccagct aattcagcga catcagttaa
      901 ttttgctacc attttaatat tttaattcaa agtattttcc agtaggttcg cctgatttga
      961 tttgaatacc attttggtga ttttcaggga atacacgact agtaaacact ttttctcctt
     1021 tattaataaa aatctcaatg attgagttat ctacaaaaat gttagcagtt gtttctttag
     1081 tatcaattga gcattcacga atcgtaccaa agtcagtggc atattgaatg cctgctttgc
     1141 tgcgatctaa aataattttt ccttctttgg tatctacaat caagctaagt ccatttcctt
     1201 tactatcaga gaaaagaagt aactccgatt tctgatttgg aggaaaagtt agttcaagct
     1261 cataagtatt atttgtttct gcttttgctg taaattgttc agaactagca cgtaatgatg
     1321 taatagcttc tactggatat tggtaaagtt taccatcttt tattgaaagt tcttttacaa
     1381 gactcattgc tccctgataa tcatacttat cagttggata atcgatatca ggtagtccaa
     1441 tccaactgac aatcagagca cgaccatcag gagtattgaa tccttgagtc gcatacgctt
     1501 caaatccata atcaagattt tgaatttctg aagcgcccac aagttttact ttttctatgt
     1561 caagcgattg acagatctta aaagtattcg gatagatatt cccataattt agctcagatt
     1621 tatccaaccc ttgaggacaa taaatgagaa caggtttttt gtctacaaat actagatttg
     1681 gacactcaat catgtattct gaaccagtgc cgccaaaatc aaggttgcca acttcctccc
     1741 atgtttccac gttattgtca tccgctttgt aaagtttgat aaagcctgat ttttctaaat
     1801 tttgtgctcc aacaatcgca tacaattgtc cttcataatt aaacatttgt ggatcacgaa
     1861 aatgatcagt tacatcagca ggtttatgaa taagaacatt ctcacctttc gtaatgttgt
     1921 agtctttatc catccatgct ccgatttgaa gtgtatcacg ttcccaattt tcatttcgaa
     1981 cgtttcctgt atagagtaag aatagattcc cctctatttc atatgcagaa ccggaataag
     2041 cgccgtggct atctcgtttt gtgtctggaa gaagttctgc tcctgttaca tgaaaatgaa
     2101 ctaaatcatc tgattcattg tgtacccatt ctttcaaccc atgagcagct ccaaatggcc
     2161 aattttgata aaaaagcgta tattttccgt taaaaaaaga aaaaccatta ggatcgttta
     2221 acaatcctgt tttaggttcg atatgatagc tagcacgcca aggtgatcgt tttacattat
     2281 ccttaatttt atccatctca tctttagtcc aatcagaata agcacgatat cgtatttctc
     2341 gaggtaagtt catattttac tccttcaatt tatcttttta caactagtat ggtaaacgtt
     2401 ttccgttgaa aaatcaatca tttcatttaa aaaaataaac tatatgacaa acgtttgaca
     2461 tatttttaag atatgctaaa ataaatttgc atttcagtga accgttttca cgtaaaaata
     2521 aaaaaggagt gattcgaatg aattacagta aagtagcttc cgacgtaata caagcggtcg
     2581 gaaaagataa ccttattgca gctgcacact gcgctacacg tcttcgtctc gtactaaaag
     2641 atgacacgaa agtgaatcaa aaagctttag acgaaaatcc agatgtgaaa agcacattta
     2701 aaattgatgg acaatatcaa gtgattatcg gtgcaggtga tgttaatttc gtttatgacg
     2761 aacttatcaa aaaaactgga ctttcggaaa tttcaacaga tgatttaaaa caaattgcta
     2821 gtaaagataa aaaattcaac cccattatgg cattaattaa attattatca gatatttttg
     2881 ttccaattat acctgccctt gttgcaggtg gtttattaat ggcattacaa aactttttaa
     2941 cagctgctgg actttttgga ccaaaatcaa ttgaagaaat gtatcctgcg attgaaggta
     3001 tttcctcaat gatccaactg atgtccgctg caccattcat gttcttacca attttagtag
     3061 gtatttcagc tgctaaacgt tttggagcaa accaatttct tggtgctgca attggtatga
     3121 tcatgacaac accagatctt ggtggctcaa ctgaatattg gaatattttt ggttatcatg
     3181 tttcacaaac aaactatgct tatcaagtta ttccagtttt agcagctgtt tggatattat
     3241 ctatattaga aaaattcttt cataaaaaat tgccatcatc tattgacttt acattcacgc
     3301 cattattatc agtaatgatt actggatttt tgactttcac agttattggc cctgtaatgt
     3361 tattgttgtc taatggaatt acagacatta ttgtttggct atataacact actggattta
     3421 ttggaatggg tatttttgga ggaacatatt cacttatagt catgactggt ttacatcaat
     3481 catttccagc tattgaaact caattacttt cagcgtggac aaatggtatc ggacatggag
     3541 actttatctt tgtagttgca tctatggcaa acgttgctca aggtgctgca acatttgcca
     3601 tttggttctt aactaaaaat tctaagacaa aaagtcttgc ttcctctgcg ggtgtctcag
     3661 cacttcttgg aattacagag cctgccttgt tcggagttaa cttaaaatac cgctttccat
     3721 tcttttgtgc tttaattggt tcaggaattg ctacagcagt agcaggatta cttaaagtag
     3781 ttgctgtttc acttggttca gcaggtttct tgggatttct ttcaattaat gcaacatcta
     3841 ttccatttta cgtattgtgt gaattgatta gctttgtagt tgcttttgca attacttact
     3901 tctacggtaa aacaaaagct gctggtatct ttaaagcaga agaaattgct gaacaaacac
     3961 cagttaattc ttcaaaagta gcaacaacag atccaatatc tactgatgtt gaatcaacac
     4021 aaacaaaagt ttcatcagaa acaattgtta gtcccatttc tggtaaagtg attagtcttt
     4081 cttcagtaaa tgatccagtc ttttcatcag aagctatggg aaaagggatt gccattaaac
     4141 caaatggaaa cacggtccat tctccagtag atggtactat tcaactcacg tttgaaactg
     4201 gacatgctta tggtattaaa tcaaataatg gtgctgaagt tctaattcat attggtattg
     4261 atactgtatc aatgggagga aaaggattta cacaaaaagt tactactaat caaaaagtta
     4321 gaaaaggcga tattcttgga gcatttgacc gttcaatgat tgagaaagaa ggtcttgatg
     4381 atacaacaat gattattgtg accaatactt cggattattc agaagtaaat atagtagctg
     4441 aaaatacagt cactgagggt actgatttac ttgaaatcaa ataaatgtat ttccctgagt
     4501 aaagatggaa ttttctttcc aaaatcaaat aatatagatt aatcaaatat ttgagaataa
     4561 cattttattt aatcttttca tatatcaatt gtatgaaaaa ttattagaat tttaaaaaat
     4621 gataaaaatt caaaaagaaa aaatagtagt tatttacttg tcatgttact aaataaaaaa
     4681 ataatttaaa tatgatatat agtcatttat ttagtgattt cattctaaaa taaagggaga
     4741 aatgtctatg ctatacggtg caattgaagc aggcggcaca aaatttgtgt gtgccattgg
     4801 cgatgaagaa atgacgatca aggaaagagt gagctttccg acaacgacac ctgaggaaac
     4861 aatgccttta gtcattgact tcttcaaaca ataccaagca gatttagcag gaattgggat
     4921 tggctcattt gggcctatcg atatccatcg cgattccgcc acttatgggt acattacctc
     4981 gaccccgaaa ttggcatggc aaaattttga tttcatcggt accatgaaaa aagaatttcc
     5041 gatcccgatt tcttggacaa cagatgtcaa tgcggctgcc tatggtgagt atgtcttcgg
     5101 cagtggcaaa ggattgagca gtgtcgttta ctatacgatc ggtacgggtg ttggcggcgg
     5161 tgccttacaa gaagggcgct ttatcgaagg gtttagtcat ccagaaatgg ggcatatgct
     5221 cgtcgtccct catccaaaag atgactttgc tggcagctgt ccatttcatg gcaactgttt
     5281 agaagggatg gccgctggac ccgccatcga aaaacgctta gggaaaaaag gacaagaggt
     5341 tgcagaagat gatccttatt gggaaatcga agcctcctat attgctcaat gtgcctacaa
     5401 tacaaccttg atgttttctc cagatgtgat catttttggc ggtggtgtga tgaaacaacg
     5461 ccatatgctg caaaacgtcc acgacgcatt tgcaaaattg gtcaatgggt atgtcgaaac
     5521 acctgagtta agcaaatata tcgtgacccc agcattagaa gacaacgctg gaacgctcgg
     5581 ttgtttggca ttggctcgag aagcggtctt acattcataa acgaaaaaag gctcttcgac
     5641 atttagtgtt ggtagagaaa gatacaactg aaaatttagt cataaccagt taggcagagt
     5701 gcttaactgg tttttgtttt acttggaaga tcaagccttg aatgagataa attcttgctc
     5761 gaaagttatg gaaatttcga tagccgtagg ccacttgtat tgtgcaatag ttttttacaa
     5821 aaaaaagggg gtgcggatat ttttttggac ctttttaaca aataggttct gttgcaaagt
     5881 tttaaatcta ctatcagata aggtagaata atagaaaaag atagcaggag gaatgacgat
     5941 gagtcatttt aaaggaaagc aatttca
//
LOCUS       DAYLZO010000144         5887 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.149, whole genome shotgun sequence.
ACCESSION   DAYLZO010000144 DAYLZO010000000
VERSION     DAYLZO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.149"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            32..655
                     /locus_tag="ACGVJG_002700"
     CDS             32..655
                     /locus_tag="ACGVJG_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558606.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1105..2154
                     /gene="trpX"
                     /locus_tag="ACGVJG_002701"
     CDS             1105..2154
                     /gene="trpX"
                     /locus_tag="ACGVJG_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFJ6558607.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2151..3032
                     /locus_tag="ACGVJG_002702"
     CDS             2151..3032
                     /locus_tag="ACGVJG_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558608.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3035..3805
                     /locus_tag="ACGVJG_002703"
     CDS             3035..3805
                     /locus_tag="ACGVJG_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558609.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4084..4938
                     /gene="aroE"
                     /locus_tag="ACGVJG_002704"
     CDS             4084..4938
                     /gene="aroE"
                     /locus_tag="ACGVJG_002704"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFJ6558610.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5116..>5887
                     /locus_tag="ACGVJG_002705"
     CDS             5116..>5887
                     /locus_tag="ACGVJG_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFJ6558611.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLD"
BASE COUNT         1990 a         1008 c         1274 g         1615 t
ORIGIN      
        1 ataaaaaata ggagaaaaag tagaaaagaa ggtgtttgtt atttgggaag tagttaaatc
       61 gagagtacaa gaaatgaatg aacatttctc agtgtcattc gcagaaaatc caagtaagta
      121 ttttcgacgt cttgtttttc tgctcatctt atcattgctg ttaacagttg caatgttttt
      181 attgccggaa aaaatccgag aagagcaagt aaatagtctc gtttattcgt ccataccaac
      241 cagcaaattg aaaccttttg attacagcaa agctgatcag aaaatcaaac aaagccgggc
      301 gatatcggtg atgttcagca aaccgaacgg gaaaacctat caggacatgc ttgatgtttt
      361 taatgatcct gagcttatgg aagaattgaa tcgaccgatc ttttattatc cgatcgttta
      421 tgatgtacat gaactggaac aaaaatataa catacgaacg gatgaagtga catttatttt
      481 ctttgatgga ggaaaagaag caaatcggat aactgcgggt aagggaggaa taactgattt
      541 tgataaggag ttgatccctg aactgaatcg acttccgtta gccaatatca aacaattaga
      601 agaagaattg gatagaacgg gagtaacttc ccaaaattcc gagattgcta attgagacat
      661 tggcgcgatg aaacggtggg tctatatagc gctattttta attatcagaa caagtgatgt
      721 ttgatcttca aaataacaaa taaaaaatta aaaaatatag ttgacgaaat tcaaaaattc
      781 gattagactt aaatcaattc ttaaggaaag ggtgacctgt aatgaactta tcaaaacgta
      841 tttcagcaga aaacaaaaaa aattggcgca gctggcgtag ataagttgta ttaatggtga
      901 gactttccca tagatacaac tttttagagt gcatttctaa agcttgtatc tatgctagac
      961 atgaacattg actgccgcat agattagaat ctatgcggtt ttttgtgtaa agacaatgac
     1021 cgctttggcc attgtctttt tttgtagact ggatacttgc ctgattataa taaataaaaa
     1081 gattgacttt gcaggaggaa aaaaatgaaa aacaaacgat taataacagt tgtagtaatt
     1141 attttagttt atttagcagg tgcatttatt tatggaaaag cctcaggcga aaaagaaagt
     1201 acttcgacga accagacaaa aaaaacaaca gttgttgggg tattgcaata cgtgagccat
     1261 ccggcattag atgagattta tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa
     1321 gaaggagaaa atcttgagat caaatttcaa aatggacaag ccgatcagag caagctagcc
     1381 acaatgagtc aacagctagt acaatctgat ccagatgcct tgattggaat cgcaacacct
     1441 gccgctcaat cattagcaaa cacgacttca tcgatcccag ttgtgttagg tgctgtaacg
     1501 gatcctgtgg gggctggatt agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt
     1561 gtttctgatc agccgcctgt tacttcacaa atcaagcttg cttctgattt gttgccaaat
     1621 gcaaaaaaag tcggaatctt gtatgcttct tcagaggata attcaaaata ccaagtagca
     1681 caagcagaag aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc
     1741 actaatgaga ttgcacaaac ggttcaagtg atgagccgac aagtcgattt tatctatgtt
     1801 ccattagata ataccattgc caatgcgatg caaacagtgg taaaagaagc aaacaaagca
     1861 aatatccccg tcattccatc tgtggataca atggtggaac aaggtggact agcaacaatc
     1921 gggatcaatc aatatcaatt agggcttcaa agcggtaaga tggcggcaga acttgcgagt
     1981 ggaaaagaaa aaccggaaac gacgcctgtt tatacatttg atcaagggga tacagtgatc
     2041 aatcaatctc aagctgatcg cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc
     2101 aaaatcatta cagatgactc gcagcaagaa acatctaagg aggatgacaa atgatcgtat
     2161 cagctatttc acaaggcatg ctttgggcaa tattaggatt agggattttc atgacttatc
     2221 ggatcttaga ctttcctgat atgacgacag aagggtcctt cccattaggt gctgctgtct
     2281 gtgtgacagc tattactcat ggggtagcgc cgattgtagc tacattatta ggcgtaggtg
     2341 ctggcatgat agcaggactc gttacggggt tactttatac aaaaggaaag ataccggtta
     2401 ttttagcagg gattttagtg atgtcaggat tgaactcagt tattttgttt gtgatgcgaa
     2461 caccaaatct ttctttattg aatcatccga ttctacaaga cagcttccat cgctttgggt
     2521 tacctgatta ctttgacaca gtccttcttg gcttcatctc tttgggcatc ttgattgctg
     2581 tactgctatt cttcttcaat acagatttgg gtcaaggcta tattgcgaca ggagacaacg
     2641 aaacgatggc tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta ggtttaactt
     2701 tgtcgaacgg tgtcattgca ttatccggtg cgctgattgc ccagaacgac ggatatgcag
     2761 atgtaaataa agggattggc gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag
     2821 tgatcttcca agaattgacg ctagcagaaa gattgattgc tattgttgtc ggcagtatca
     2881 tttatcagct attgatttta gtagtaatca aactagggtt tgacacgact tatctgaagt
     2941 tgttctccgc tatcatctta gctatttgtc tgatgatccc gcaattgaag aaagcactga
     3001 acttaaaaac gggttttgag aaggaggcat agtaatgact gatatattga caatcaaaaa
     3061 tggcagaaaa gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg atgaaattaa
     3121 ccttacaatc agagaagggg acttcatcac tgtccttggc gggaatggtg ctggaaaaag
     3181 cacactgttc aatacgatcg ctggtacgct tcaactcagt caaggatcga tctcttttaa
     3241 agatcagctg atcacaaaag attcagaaga aaaaagagca cgattccttt ctcgagtttt
     3301 ccaagacccc aagatgggaa cggctcctcg gatgacagta gcagaaaatt tgctacttgc
     3361 acaaaaaaga ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga
     3421 ttttttcaga ctatgtcaag aagtcggtaa tggcctagag caacacttgg atactccagc
     3481 gggtgaatta tctggtggtc aacgccaagc attaagcttg ctgatggcga cgatcacagc
     3541 tcctgagtta ctattattag atgaacatac tgcagcttta gatcctaaaa cagctaaagc
     3601 attaatgcga ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac
     3661 gcatcgaatg gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa
     3721 aatcgttcaa gatatttctg aaaaagaaaa gcaacatttg acaatgggcg accttttgca
     3781 gttctttgaa gaagtagtgg attgatttcg acagaaaaat tcctttcagt agaaggactt
     3841 gaaaactcat tgacatcttc ctttgattca tcctatactt aaaacaataa acaattaaac
     3901 atatttgggt gttttgtagg atcacgtagt caatgatttt aacaattaaa tagaatattc
     3961 tttgctctga caagtaaacg atgagaaaag aatttgctac tttgccacct agacgtttcg
     4021 tactttttcg tatcaaacgc taagtggctt tttttattga agtaagaaca aaggaggaat
     4081 aagatgattt ctggacaaac caaattagct gggctctttg ctgatccggc ttctcacagt
     4141 ttatccccat tgatgcacaa tactgctttt gaagcaaaca acatcgatgc ggtgtattta
     4201 gctttccaag taaatccaat gaatttgaag caagcagtcg aaagtattcg aacttttgat
     4261 atgttaggtg taaatctttc gatgccgaat aaaacagcgg ttattcctta tctggatgag
     4321 gtaagccagg aagcgcaatt gatcggagca gtgaatacga tcgtgcacga aaagggtcgt
     4381 ttgattggct ataataccga tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt
     4441 tcgatcaaaa agaaaaaaat gactctactg ggagctggag gtgcagcaaa ggcagtcact
     4501 gttcaagctg cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca
     4561 ttcgctcaag taaaagaaac attcgaacaa ctcagctatc gaacttcttg taagataaaa
     4621 gttcgtgact ttgccgatac gcatcaatta agaaaagact tgaaagaaag ctgtcttcta
     4681 gtcaatgcaa cagatatcgg gatgggaaag aaaatcaatc agtcaccgct cttagatata
     4741 agcatgatgc atgagaatct agcggttttt gatttgattt atgcacctag agaaacacgt
     4801 ttattaaaag aagcaaaaaa agtaggagca aaaggataca atggacttgg catgttgatc
     4861 taccaaggag caatcgcgtt tgaattatgg acaaaggaaa agatgccgat ccatgtgatt
     4921 gaaaagctgt ttgattaaaa aaggataata gataaagatc ttttattctc gcataaacgg
     4981 tctcaagaaa agtaatcctt tatgtatcga agcatttatt tcgttatttt ttattaaatt
     5041 atcaaaaaat gataggcttt tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa
     5101 cgaggaggaa ccacaatgag tcggatggat cgctataagg acatccataa gaaggcgaaa
     5161 cctctgaagc gggatacggg gagtattttt gctagaagag aacgaaaacg aacgcgtatg
     5221 gaaaatgagg aagatgctac ccgtatttat gaaagagagc agcattcatt tgaacatttg
     5281 aaccaatacg agcaacagat atacagagat gcaccaagag cagaagaaca acaaaaaaat
     5341 aaaaaatttt tcaatcggca aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg
     5401 tggaaaaaaa tcattctatc gattttgctt gttttgatcc tttattcagg tatctcattt
     5461 tttattggta aatatgtagc tgaacatgac acaagcttag aggcagcgac ttcagaaacc
     5521 tttcatgggc aaaaagcgac gaatggtgcg cataatattt tgatccttgg aagtgataca
     5581 cgcggagaag atgccgggcg agccgatacg atcatggtct tacagttgga tggtccttcc
     5641 cataagccga aactgatttc ttttatgcgt gacagcttcg ttacgatccc aggggtagga
     5701 caaaataaga tcaattcagc ttatgcatac ggaggagccg atctagtgcg tcaaacttta
     5761 gtagaaaatt ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa
     5821 aaagtgatcg atgccttatt catgaacgga gtgaagatcg atgcagaaaa agatctgaat
     5881 ctggatg
//
LOCUS       DAYLZO010000145         5874 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.150, whole genome shotgun sequence.
ACCESSION   DAYLZO010000145 DAYLZO010000000
VERSION     DAYLZO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.150"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            641..1375
                     /locus_tag="ACGVJG_002706"
     CDS             641..1375
                     /locus_tag="ACGVJG_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6558612.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="ACGVJG_002707"
     CDS             1391..2560
                     /locus_tag="ACGVJG_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFJ6558613.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="ACGVJG_002708"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="ACGVJG_002708"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFJ6558614.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="ACGVJG_002709"
     CDS             3650..4576
                     /locus_tag="ACGVJG_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFJ6558615.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="ACGVJG_002710"
     CDS             4595..5845
                     /locus_tag="ACGVJG_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFJ6558616.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1966 a          955 c         1203 g         1750 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gtta
//
LOCUS       DAYLZO010000146         5741 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.151, whole genome shotgun sequence.
ACCESSION   DAYLZO010000146 DAYLZO010000000
VERSION     DAYLZO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.151"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            598..1287
                     /locus_tag="ACGVJG_002711"
     CDS             598..1287
                     /locus_tag="ACGVJG_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFJ6558617.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="ACGVJG_002712"
     CDS             1274..2482
                     /locus_tag="ACGVJG_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFJ6558618.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="ACGVJG_002713"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="ACGVJG_002713"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFJ6558619.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..>5741)
                     /gene="guaB"
                     /locus_tag="ACGVJG_002714"
     CDS             complement(4596..>5741)
                     /gene="guaB"
                     /locus_tag="ACGVJG_002714"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFJ6558620.1"
                     /translation="EELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIAD
                     VMTKDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNA
                     AKDEHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTF
                     ADATLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAAS
                     VARQYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKT
                     YRGMGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSG
                     MGYVGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1762 a         1077 c         1117 g         1785 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt c
//
LOCUS       DAYLZO010000147         5717 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.152, whole genome shotgun sequence.
ACCESSION   DAYLZO010000147 DAYLZO010000000
VERSION     DAYLZO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.152"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..1342)
                     /locus_tag="ACGVJG_002715"
     CDS             complement(<1..1342)
                     /locus_tag="ACGVJG_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFJ6558621.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKE"
     gene            1944..3014
                     /locus_tag="ACGVJG_002716"
     CDS             1944..3014
                     /locus_tag="ACGVJG_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFJ6558622.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            complement(2980..3066)
                     /locus_tag="ACGVJG_002717"
                     /pseudo
     CDS             complement(2980..3066)
                     /locus_tag="ACGVJG_002717"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(3110..3571)
                     /locus_tag="ACGVJG_002718"
     CDS             complement(3110..3571)
                     /locus_tag="ACGVJG_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFJ6558623.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            complement(3831..4379)
                     /locus_tag="ACGVJG_002719"
     CDS             complement(3831..4379)
                     /locus_tag="ACGVJG_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558624.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(4380..5237)
                     /locus_tag="ACGVJG_002720"
     CDS             complement(4380..5237)
                     /locus_tag="ACGVJG_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFJ6558625.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            complement(5523..>5717)
                     /locus_tag="ACGVJG_002721"
     CDS             complement(5523..>5717)
                     /locus_tag="ACGVJG_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFJ6558626.1"
                     /translation="YDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRVAYEIIENKV
                     IPVLLCGTHENFYEQLKKLI"
BASE COUNT         1771 a         1120 c          887 g         1939 t
ORIGIN      
        1 cttctttatc tttataaaga accaacgctg cagcaaaagc aaccacaaat gaaatccccg
       61 atactgcaaa tgcccaaacc agattcattg ggtcagcttt gtcagcgaac atcgtgatac
      121 ttgcaatccc aggacccact aaagcaaagg cttgaagtgc aacgattcct gcaaatgctc
      181 ccccgatcag gctagaaaac ataacggcat ataaaacacg tttgtgcaga atcgttaccc
      241 cgtaaagagc tggttcagta attccgaata atgcagatat acccgcagaa acagccgtag
      301 atttcaattt tttgtctttt gttttgatac tgacagcaaa gcatgcgcca gcttctgaaa
      361 tattgtgtgc caatgaagcc gggagataaa gaagttctgc acccaattca ctcatggatg
      421 aaacagcgta agggatcatt gctttatgca tacccgttac aaccattaat ggcagtacag
      481 ctgctaagac tccggttgca acccatccaa attgattgta caaagacaca atgatagaag
      541 agaaagctgt tcctacatta taacctaatg gtcctagaag caacaaagca ataggaactg
      601 taatcaataa gctcatcata ggtacaaaga aaatacgaat tggttttggt gaaacttttg
      661 taaaccattt ttcaagttga gcataaaata aaacagttaa gatggctggg aatacttgag
      721 atgcatacgc tatattatcc aatccaaaag ataagaatgt agcaccttcc gccaacatag
      781 ctgtcatttc aggaaggatc aatgcaccga cagcagatac tgcaaccaat tgattgactt
      841 tcaatttatt agccgtagtc attgccacca gaataggcaa gaaataaagc ggtgccccgc
      901 cgaccaaatt taaaatctga taggtttgag attggtcact aatcagccct aagatagaag
      961 ctagtagcaa caaggacttc aatactcctc cacctgcaat cgcaggaatt aaaggttgga
     1021 aaatagaaat aataaaatct aatagaactg aacctaattt ttgtttttgt tttggtgctt
     1081 cttcagtatt tgaaacattg tcacctaata acgctttcac ttcatcatac acttcaacaa
     1141 catcgtttcc aatgattact tggcattgca cattttggcg aacgcccata acgccatcca
     1201 ctttttctaa ctggctcata tttaccttgc tgttgtcttt taacgtaaaa cgtaaacgag
     1261 ttgagcaatg ctccaaatgg gatatgtttt cttccccacc gatgtactgc aatacttctt
     1321 ctgctgtttg tttgtaattc atggtgtttc cctcttttct tttttgagtg atgaactttt
     1381 gagtcgaagt ggccatcctc aacacaaaaa acaggcagaa actaaataaa ttgacaacaa
     1441 ggcaaacgtg tattgaaacg catacattgt tattcaactt atttaggtcc tgcctgcatt
     1501 accagtcaca tgcctgttta agttatattc aatttaacat attagaatgt aaacgtaaat
     1561 aataaaatga ttttttaatc attttttcat tttattcgtt caatatagtt ttcgtgttct
     1621 aaaagttttg tcttttttct aagaatatct tccaacatat ataactctta ttctcttcta
     1681 tcttctagca gatgttctgt ctttttaaga cctagtataa aaagaatggg ttcatttttt
     1741 ctttcttgca ggatgaggtt ttttatataa actttatttg atataaagaa aaggcattca
     1801 taattgtgct atactactaa aaaatgataa tacagtgaat tagattagcg ctttattaaa
     1861 ttaagaggac agcatatcga tattgcttcc ttttttttgc tatttattta agggatagaa
     1921 gtttagaaag gactgtttat aagatgaaaa atgatatagt gacttatgca aacacaaaat
     1981 gtgaagaaat tggcatcagt aatttagaag agtgcttttt tgacacaaat gacctgtcgc
     2041 aatttttaag aacgggaaga acaaatgcca gtaaacttgc caatgactta gttgcaaaga
     2101 acgttctatt taaaatcaat actcgtccag tgttatttgc catcaaagag cgctttgaag
     2161 agcacttttt gattccggta aaagatgtat acgaaagtat atctgaatta aaaacagact
     2221 tggagcccaa ttctacagat tccattctac aaaagatgat tgggtatgat atgagcttat
     2281 ccgatgcggt ggaacaaatg aaaactgctg tcttctatcc tggaagaggg cttcctatga
     2341 tgatcactgg agatactgga gttggaaaaa gtttttttgt tcatactgct tatcagttta
     2401 tgaaaaaagt gaacgttatt catgagaatg ctccttttaa agtacttaat tgtgcgcaat
     2461 accataataa tcctgaatta ttgtcgggga tactatttgg ctatgttaaa ggtgctttta
     2521 ccggcgcaga tgaagatcga aatggattgt tgcaagatgc agacggtgga gtactgtttt
     2581 tagacgaagt acacagattg cctcctgaag ggcaagagaa actatttgtt tatatggatg
     2641 gcggaactta ttctagagtc ggtgaaacta tccatcacgc ctccaatgta cgtttagttt
     2701 ttgcgactac agaaggcaaa gaggattttc tggaaacctt tatgcgaaga atccctatac
     2761 atatccatat cccaaatatt gaggaacgag gtattttaga aaaaaacaaa ttatcgaaaa
     2821 tactctcttt agcgaaagca aaaactttag caaggctatt gatgtttctc ctaaagtcac
     2881 tagtgttgca taaatatagt cagaatattc tgaattatta ttttcttgaa aaaagtaaaa
     2941 aaaatcgtat cccaaacttt catctacatt tccagaaaga tcttcatgtt gataatcaat
     3001 tcctgaatca atgaccgcta ccttcacatc cttagaaccc ttggtttctt cccaagcgga
     3061 tggcatgcta atctctctta aattagcttg tttactccaa ttaggatcgt tatcgatttc
     3121 ctaaagaatc agttgtattt ggctcctcaa catcattcaa cggctctagt tcaaccacat
     3181 agtttggctc tgcataattg accatatcag aagcattaaa tgttcttact gcctcttcca
     3241 cactaatttc ttcgttcaat ttcactaata atacttgatc tcgcttatta taatcgcttt
     3301 catttgtcct aagagtcaaa tctttttcca ccataacttt tacacccaat aaattttgcg
     3361 gtttattttt ctctgttgaa attctttgtg tagcctttaa actgacaatt agctcattgg
     3421 gtacatactc tatttgagag tcatctaatt ggctcataat cctttctttt tggttagtat
     3481 ctgcagttac ttgtttagta tgagccatga acccaaacat actcacccca acaaccaatc
     3541 ctaaatatat tttcttttta aactttttca tctacatctc ctcctaatag ttttatttta
     3601 gagttagctc tattacttat ttactaactc aacaacagat tatcatacac ttattaatga
     3661 aatcacaata tctcattaaa acacacccta aaatcataac cgttcctatg ctcatttttt
     3721 ggatttttta atcacgtaac cagaaaaata aatcccctct atttcacgca aatcaaagaa
     3781 gaactaagta actaccgttt aaaggtagtt acttagttct tctttgatta ttatctaact
     3841 ttattattca ttccccaccc tttgcgcagt gagtcttcgg tatcttgttt tatctgactg
     3901 gtagttctta ttatttctcc acgatcatct aattctggaa caattggata tttttctctg
     3961 aacaagtcta gttctttcac cacgtcatta aacaaatctt gtttatcttc ttgcgtaaga
     4021 ttctgtttaa tatactcatc taaagctttt ctaagcaatt catctcttgt catattagtc
     4081 tcatactttt ctactaaaac tctggaagtc gcatttaaca aagtcgacag ttttaaagga
     4141 tgcttggtgg aataaacctt atcaaacttt ctgattggtg cgccaacttt ttttgttctt
     4201 cctctactct gtctttcaac attggaggaa gtttttattt cttgaacttg gtgcacttct
     4261 aattgcttat tttcagatgg tgtaattgct tgctgctttt tctttgcttc tgcttctttt
     4321 tttattttgg cgagtccttt gttgttccca aaaatatcaa gtcctttgat gtcagccaat
     4381 tactccacct ccacatacca attctcgtgt tcttccaatt ctttcaaaat actgataaat
     4441 aattcgtggc ttttgcgatc ccattgtttt aagagacctt tctttgtctt ctccatcgta
     4501 atacctgatc cgtcgtatcg ttttaagcgc gcatttttga gtacaacatt tttaatcaaa
     4561 tgttccccat aaatctcttg ggcttgttta tacatttcct gatctactgc gtcaccgctt
     4621 tccaacatga aaggaataac accagcaaca tataaatttg tatcaaattc tttcacaaaa
     4681 aagtccatat actcaagata ttgcatcgca tttctcatcg attttacttg ggtctgtaag
     4741 acgacgattg cccaatccgc aatgtacatc gcggatttac tatatacaga aatagaaggt
     4801 gggacatcta aatagatgac gtcaaactca tcaaataatg gttctagctt ttctttcata
     4861 tatttgaatt gggataatga atcattatat tggtctttga tccaatcatc atacatttca
     4921 aaggacatgt ctgctggaag tatatagagg ttattgatta ctttatagac agcctcttta
     4981 ggtggaagat tgtttatcaa cacgtcagtc acagtatttt cgtaaacaat cggttcacct
     5041 tctccgcctc cttcttcgtt agtgaagttc atatgcgtta agttcatcac atcagaagta
     5101 tttccttgca tatccaagtc aattatcaag gttctttttc cttcattaac tgctgattca
     5161 aaccctaaca tttgcgcgca cgtactttta cctacgccac ctttaaaatt accgattaca
     5221 tatttcttcg ctaccacgtt cctacctctt ttcttttagc aatattcatt tgatactatt
     5281 ataacatatt tatttttagt tactaccaaa aaaaagatat atatgtacta ccttataatt
     5341 aataagtaaa aggtagtaca taaataagga aaagctgaaa tatattgatt tatagctttc
     5401 ctcaaagtaa tgacgttatt taatttatcg agaagcagag aatactcgcg actttagtca
     5461 tgtagacgaa tcagattttc gataaaaaag aagccgtcct tcttaagaac gacaacaaat
     5521 cactagatta attttttcaa ctgttcataa aagttttcat gcgttccaca taacaatact
     5581 ggaataacct tgttttctat aatctcgtaa gctactctat atgttgtgcc cgcatatttg
     5641 aacccactag accaaatacc tgataaatcc ccagtttttt gttcaccctt atatggatca
     5701 atcgcaattt catcata
//
LOCUS       DAYLZO010000148         5710 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.153, whole genome shotgun sequence.
ACCESSION   DAYLZO010000148 DAYLZO010000000
VERSION     DAYLZO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.153"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..194)
                     /locus_tag="ACGVJG_002722"
     CDS             complement(<1..194)
                     /locus_tag="ACGVJG_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558627.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(737..2887)
                     /locus_tag="ACGVJG_002723"
                     /pseudo
     CDS             complement(737..2887)
                     /locus_tag="ACGVJG_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3033..3608
                     /locus_tag="ACGVJG_002724"
     CDS             3033..3608
                     /locus_tag="ACGVJG_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFJ6558628.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3822..4517
                     /gene="vanR-A"
                     /locus_tag="ACGVJG_002725"
     CDS             3822..4517
                     /gene="vanR-A"
                     /locus_tag="ACGVJG_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFJ6558629.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4495..5649
                     /locus_tag="ACGVJG_002726"
     CDS             4495..5649
                     /locus_tag="ACGVJG_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFJ6558630.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1786 a         1096 c         1083 g         1745 t
ORIGIN      
        1 tgttcaaaat ctaaaatcaa cacatcatgg ggagtgtgat aataaatatt ttcccttaat
       61 gtaccaacaa tggtaattcg gtcaattgac cattgcattt ttaagggggt gcacatagtt
      121 tgtgcattta accgcctgtt agaaatacgg ttaagttttt cccactcatt tacgattatg
      181 ccttttttac tcaaaaatag ctcctcctca acgaaaaaag agattcttgg aaagaatttc
      241 cagagttcac gtaactctaa aaatccacat ccaaaaatct ctagcttgaa gaaacctaaa
      301 aatattcgta taatgaactt gagttacgga actaggtgaa gtagtttccg taaaggtcga
      361 gagtgcgtca acactgctcg actatttttt tactcttttt tctcattttc tttgattata
      421 gcactcctct agaaaaaata aaaagacctc agggaatttc attccccgaa ccccttgagc
      481 ctcctcctgc cgaaatagaa tggcgctgcc agcatttatt tcgttcagct atcggctaca
      541 caatctaaca cgaccgttcc gctaccagtc gccttacagg ctccattttc gctcctctaa
      601 cgtgtttcac cgattgtgcc gataactaaa cgaaataaac gctaaaaccg ctcagaacgc
      661 aaaatatgcc attttagagc tattgtagaa aatatagttt gaaatgcaat tttcttcttt
      721 cagcttttta tttcccactt gtaaatcggt agatggtggc tccttttcca gttccataat
      781 gtggatttgc atctgcatgt agtgatgaaa cacctagctg cattctcata ccatctgacg
      841 aagatgttgt accgtcgccc caatagaaag gcaattgtaa tttatgatga aagtttacta
      901 atatggcttg ggctttattc atggcatctt catacatgcg ccattgagat acattggcta
      961 gttgcttata tgtaagtccg ggtgtggctt cggccatctt gctcaagcca atattcattc
     1021 ccattcctaa aagggcagcc atgataatga ttgtttcttc cttatctggt tttcgattat
     1081 tggaagcatg agtgaattgc tcatgaaatc ctgttatatg ggccacatcc atgagtaaat
     1141 cagttaattt tattcttggt agcatctgat aaaggcttgc actaaatttt tttgcttctt
     1201 ctggaacatc tttttctaag cgtgcaagtg atagctttcc tttttcaaga gaaaccccat
     1261 ctaacttatt ggaattggca gctaaccact ttaacctttc attaaagctg ctggttctct
     1321 ccgttatata atcttcgaat gataaactaa ctgataatct cgtattcccc ttcgattgat
     1381 tccatgtatc ttccgaaaac aaatattcct caaaatccct atattgtctg ctgccaacaa
     1441 tggaaacatc tcctgcccga acatgctccc gaagttctgt taaaacagcc atttcatagt
     1501 aatgacgatt aattgttgta ccatcatcct cgtataaatg tcttttccat cgttttgaaa
     1561 taaaatccac aggtgagtca tcaggcactt ttcgctttcc agattcgttc attcctcgga
     1621 taatctcaac agcttgtaaa agtggctcat ttgcctttgt agaatgaaat tccaatactc
     1681 ttaatagcgt tggcgtatat tttcttagtg aataaaaccg tttttgcagt aagtctaaat
     1741 aatcatagtc ggcaggacgt gcaagttcct gagcctcttc tactgaagag acaaaggtat
     1801 tccattcaat aaccgattct aaaaccttaa aaacgtctaa tttttcctct cttgctttaa
     1861 ttaatgcttg tccgatgttc gtaaagtgta taactttctc atttagcttt ttaccgtttt
     1921 gtttctggat ttcctcttga gccttacgac cttttgataa caaactaagt atttgcctat
     1981 catgaatttc aaacgcttta tccgttagct cctgagtaag ttgtaataaa tagatggtta
     2041 atatcgaata acgtttattt tcttgaaagt cacggaatgc atacggctcg tatcttgagc
     2101 ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg actaatttgc actgtttcta
     2161 aatccattcc tcgtatgtat tcgagtcgtt ctattatttt tagaaaagtt tcgggtgaag
     2221 gatgacccgg tggctctttt aaccaaccca atatcgtttt attggattcg gatggatgct
     2281 gcgaggtaat aatcccttca agcttttctt tttgctcatt tgttagagat ttactaaccg
     2341 tattaaatag cttcttttca gccattgccc ttgcttccca caccattctt tcaagtgtag
     2401 tgatagcagg cagtataatt ttgttttttc ttagaaaatc tatgcattca tgcagtagat
     2461 gaatggcatc accattttcc aaagctaatt gatgaaggta cttaaatgtc attcgatatt
     2521 cactcagggt aaaagttaca aagtcgtatt cacttcgaat ttctttcaaa tgatcccaaa
     2581 gtgtattttc cctttgagga taatgatcaa gcgaggatgg actaacacca atctgtttcg
     2641 atatatattg tatgaccgaa tctgggatgc ttttgatatg agtgtatggc caaccgggat
     2701 accgaagaac agctaattga acagcaaatc ctaaacggtt ttcttccctc cttcgcttat
     2761 taactatttc taaatcccgt ttggaaaaag tgaagtaggt ccccagtatc cattcatctt
     2821 cagggatttg cataaaagcc tgtctctgtt ccggtgtaag caattctcta cctctcgcaa
     2881 ttttcattca gtatcattcc atttctgtat tttcaattta ttagttcaat tatatatcaa
     2941 tagagtgtac tctattgata caaatgtagt agactgataa aatcatagtt aagagcgtct
     3001 cataagactt gtctcaaaaa tgaggtgata ttttgcggaa aatcggttat attcgtgtca
     3061 gttcgactaa ccagaatcct tcaagacaat ttcagcagtt gaacgagatc ggaatggata
     3121 ttatatatga agagaaagtt tcaggagcaa caaaggatcg cgagcaactt caaaaagtgt
     3181 tagacgattt acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta
     3241 gtacacaaga tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat
     3301 cactaaaaga tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta
     3361 ctgtaatggc tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag
     3421 ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa
     3481 atcacgcagg aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc
     3541 aaatttgtga aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga
     3601 ataattagcc attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta
     3661 taaaatatta acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca
     3721 tcttaagaaa ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg
     3781 ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta
     3841 ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt
     3901 atacggtttt caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga
     3961 ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa
     4021 aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag
     4081 ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac
     4141 tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa
     4201 aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg
     4261 agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa
     4321 tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg
     4381 gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg
     4441 aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt
     4501 ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat
     4561 atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga
     4621 gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg
     4681 gacgcgatga aattatatca atattccata cggaacaata tagatatctt tatttatgtg
     4741 gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac
     4801 tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag
     4861 ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa
     4921 aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg
     4981 gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag
     5041 gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg
     5101 tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg
     5161 ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa
     5221 ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg
     5281 accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc
     5341 gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg
     5401 gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt
     5461 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
     5521 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
     5581 gataactata cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg
     5641 aggtcctaag agatgtatat aattttggct ctttgtcaaa tgatgttggt aaagagaaat
     5701 cttagaataa
//
LOCUS       DAYLZO010000149         5542 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.154, whole genome shotgun sequence.
ACCESSION   DAYLZO010000149 DAYLZO010000000
VERSION     DAYLZO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.154"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            24..248
                     /locus_tag="ACGVJG_002727"
     CDS             24..248
                     /locus_tag="ACGVJG_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFJ6558631.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            245..1270
                     /locus_tag="ACGVJG_002728"
     CDS             245..1270
                     /locus_tag="ACGVJG_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFJ6558632.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            1460..1533
                     /locus_tag="ACGVJG_002729"
     tRNA            1460..1533
                     /locus_tag="ACGVJG_002729"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1494..1496,aa:Ile,seq:cat)
     gene            2210..3372
                     /locus_tag="ACGVJG_002730"
     CDS             join(2210..2486,2486..3372)
                     /locus_tag="ACGVJG_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFJ6558633.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
     gene            complement(3502..3951)
                     /locus_tag="ACGVJG_002731"
                     /pseudo
     CDS             complement(3502..3951)
                     /locus_tag="ACGVJG_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4295..4702
                     /locus_tag="ACGVJG_002732"
     CDS             4295..4702
                     /locus_tag="ACGVJG_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558634.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            4716..5117
                     /locus_tag="ACGVJG_002733"
     CDS             4716..5117
                     /locus_tag="ACGVJG_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558635.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            5119..5490
                     /locus_tag="ACGVJG_002734"
     CDS             5119..5490
                     /locus_tag="ACGVJG_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558636.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT         1881 a          910 c         1043 g         1708 t
ORIGIN      
        1 actttattta gggagagaag aaaatgaata ataaaacgtt cgaagtacta aaatggttcg
       61 cactggtaat tattcccgca ctagctactt tcgtggggtt agttggtaaa gcgctcaatt
      121 ggcagtacac agatatctgt gttgtcatca ttactggttt tggcgcgttt ttagggagtg
      181 tgttgggtgt atcaaatcga acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa
      241 acaaatgaaa aagaaaatta ctattactgc gatgagcctg ttaacggctc tttttttatt
      301 acccattaat acgtttgctt atactattaa tgacgagtat aatttagcgc cgaatcaagg
      361 agactccaga ttagcaattc ctaacaaaat tattttgcac gaaactggaa tagatgcacc
      421 agcaagaaac gtagccgcca acatgaaaaa taattataac ggaagcaatt cttatactac
      481 agatgttatt ggtgacggtg ggattgttta ccgtgtgggt gagcaaggat atgtttcgtg
      541 gggagctggt aacgctaatc cttatgcgcc tgtacagatt gaattacagc gcacatatga
      601 taaagcattg tttgaaaaaa actatcgagc ttatattgaa tatacaagag atagtgcaaa
      661 aaaatatgga attccattga ctcttgatca aggaacttct ttatttacaa aaggaatcat
      721 ttctcatttg tgggtgacaa attatgtttg ggggaaccac acagatccat atggttactt
      781 atcgcaaatg ggagttagca aagaaaagct tgcttatgat ttagctcatg gatttaccga
      841 tgaaaatcca acaacttctg aaaacaagcc tgtcattgat ccaacacgag ctggtgcagc
      901 taatcctacg ctgacagatg gaacaaatca cgcccacatt gatcagtttg gggaaatcga
      961 aaacgcaaac ttgcatgtgg ctggatggca cattgctaac tataaatacg agtatatttt
     1021 cattatggac tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag
     1081 accagatgta aaccaagctt ataatacttc tggaaatgtt ggttatcatg tatctttcaa
     1141 tatgcgtaat tttcctagta agaaagtcta tgtaatgatg cgggcaacga atgatccaga
     1201 gggaaacact aaaggcggtg cgcaagattt ccatgacaaa cgttggtatt taaatattcc
     1261 taaacgataa aaatagctcc tcgttgagga gcagtacata actatattga caactataaa
     1321 aattattcga taaaatagtg atgttatcgc atatcttcac tatcacccat aaatagtcac
     1381 actccaagct atgcgataac aggtttgttg ccacacattc tactggttga ttgtttatgg
     1441 ctttatgtgg caacaaccag tacccttagc tcagttggtc agagcagacg gctcataacc
     1501 gtccggtcgt aggttcgagt cctacagggt acattaacgt agccatttga atcgttctgt
     1561 gttagaattt ttttgaagag tattatacaa gctaaagctt ttcttcattg ccactcaaat
     1621 gagtggcttt tttatgtatc cttttatgga ttaatgaaag gatgtttcac atagttatac
     1681 ttctgtatat ttgaaaagtt ttactttgat ttttaaatag aaagacattt gggttaaatt
     1741 gtgagataat aataaagaag agtttaaagc gcaccccaaa ccacttcccc ataagtgtgt
     1801 tacgctttaa actcttttat atttgaagcc attaaaaagc ataccatatt tttgaaaaaa
     1861 agtgagaaaa aaggcttaca attggagtgg tagttaatta gtgacttatt tttgatttta
     1921 tagcactgat actataaaat atagatatca tcatattaca caatcttaat actaacttaa
     1981 aaaatatctc ctttcacaag tatggtgata aaattcgttc cgggctacct ttttaggtag
     2041 cctactttaa tctttatacc tttctggatc aacgaaagta tactttatat agtcataacg
     2101 ccgatgatcg ctacgtgcgt ccggcacgtc agtcatgaat cggctacact agactagaca
     2161 gaaaaaataa ggtgtgtaga ataataaaaa acacactgga ggatttttca tgtcaagacg
     2221 tcaacgaaga acctattcaa aagaattcaa acaacaaatc gtcgatctct atctcgctgg
     2281 taagcctcgc gcagaaatta ttcgagagta tgagcttacg ccttcttctt tcgataaatg
     2341 gatgaagcaa gcacaatcaa cgggctcatt caaagaaaga gacaacttaa caccagaaca
     2401 agcagaattg atcgcactaa gaaagaaaaa taagcaactc gaaatggaga atgatatttt
     2461 aaagcaagcg gcgctgatat tcggacgaaa agacaagtaa ttgatgccaa caagcataaa
     2521 tattccatat cagcgatgtg caaaattcta aatatttctc gtcaaaccta ctattatcaa
     2581 gcgaaaccga tcgaaaatga gtccgactta gaagagatcg ttcaggaaga gtttattcga
     2641 aaccgaaagg cttacggtac ccgaaaattg aagaagtgtt tagcaaagcg tgggcttcaa
     2701 ctcagtcggc gccgaatcgg tcgaatcatg aaacgccgcg gattgacatc tacctatacg
     2761 atcgctcatt ttaaagggca acgaacagct tgtaatgaag cgaaaacagc gaatgtatta
     2821 gatcggacct ttacacaaga acagccattg gaagccatcg ttacggatct tacttatgtt
     2881 cgcgtgggga aaaagtggca ttatatctgc ttaatacttg atttgtttaa tcgagaaatt
     2941 attggttatt cctgtggtga gaagaaagat gcctcattgg taaaagaagc ctttggacgg
     3001 ataccgtatt ctttaacaga cgtcaagctt tttcatacag accggggaaa ggaatttgat
     3061 aaccaaacca ttcatgagat tctgaatggt tttggaatta ctcgttcatt gagtaggaag
     3121 ggttgtccgt atgataatgc cgttgtggaa tcaacctata aatctgtcaa agtagaattc
     3181 gtgcatcaat accaatttga gacactggca cagctacgtc tagaattgtt tgattatgtg
     3241 cattggtgga actatctacg cttacatggc acgttggcgt atgaaacacc gatccaaatt
     3301 cgacaacaga gattggcgaa gcgaatcctt gataatgagc gcggatctga tacctctgga
     3361 gaggcagcgt aattgaatga ttgtgcttct gccggagaaa atcagatccg aggatgctca
     3421 ttgtcaaggg caatcgtagc cataacaccg cagcattcac aacaccttat aatttttgtc
     3481 aaaaaaagtg ttgccattcc acttttttaa atatgcgttc tctgctctta aacgaataac
     3541 ttcttcttca agagactcat ctttaagttt tttaggaatg tttagcttgg aattcatact
     3601 aatttttctg cccctttttt ggctctcaag tgaagaagca ccgccttctt catattgctc
     3661 tatccattta cttagagttc tatttgaacc gatattaaat tttttagcag tttcttggat
     3721 agaaagacca tttgtttcca tatattctat aacatcaagt ttaaattttg tagtgtagct
     3781 tttgccacct ccaaccaagc cttcccaacc atgatagtta taaatcctta cccaatgtct
     3841 aaccagtgta cgatttattt gatatttatg tgcaagatat ttgtagccgc cttcgttatt
     3901 taaatagtct gaaactactt ttttcttaaa aacaaatgta tatttccgca aaaaaagcac
     3961 cccttttaat tagatttcta gtctaacttt tggggtgcac atcatttttg aggggaagta
     4021 catatagttg ttgatatagt tgctaatatt gttagctaat ttgttagtaa aactgtatac
     4081 gaatttagat agtaaaaaaa cagtttgttc cttatataga ggcactttta ctactgatta
     4141 aatgtcagat atgcttacaa atatacttat aaatatgctt acaaaattag ctaatatttc
     4201 cgttgacaaa gctttgttac tattgttaaa ctaaattgac tagcaaaagt gaaacataaa
     4261 tttattaaat aaatgtaaaa attaggagaa atcaatgtct tatagagtac aattaataat
     4321 ttcagaagat gtagaaagtc agcaatttgg cactaatgtt attaataaag taattaatcc
     4381 agctctttct attaatgcgc ctttaattcc tactgcttta tcattttctg taacagctgt
     4441 ggtgtctgag atagaggata cagaaaagat aaaaatagta gaaattgaag ttttaaacaa
     4501 gaatgaaaaa caaatttttt ctacgggtga agtatcggta agcttgcctc ctcaagttaa
     4561 cgatataaac tttaatataa acgccagaaa tgtcttggtt gaagaagcag gagagcatta
     4621 tgctgttgtt aaatttaatg gaactgagat tggaaggcaa atctttgata tcaaggtcaa
     4681 caaaccagtg gaaaaaaatt aaggagacga tacggatgct tgatatagta ccatcaaata
     4741 cacatcggag tggtagctta ataaattttg ctgcgatttt tgcttgcgca tcggtaattc
     4801 tagcatctcc agttgttgtg gatgcaagtt ctactccgaa aactaaaaat gataatcaac
     4861 ttgtaattac tacaaaaaat gagattaaca caatttcaag taatgatggc aaaatttttg
     4921 atgtgataaa tacggtttta aaaagaaaag ctgaaatcaa tataaaatat gatgaagatt
     4981 taaacttgta tttctttgtt atcaaaacaa catctgaatt gttttcctca gattacgatg
     5041 ttttggatac tctcgataat gtattatctg actataaata tatggggaaa tctgtagtag
     5101 caactttggg ggaataaaat gtttgacttt aatgaatatt tatctatttc caaacaaatt
     5161 gaatcagatg ataaatataa ttctaaagaa tcttatagaa gaactgctgt ttcaagagca
     5221 tactacagtg cttttaaaaa atctgatgag tatttgaaag aaaattatga tattatttat
     5281 aatggaagta gcggaaaagg aagccaccaa accgtttgga atttgttttc aacagttaag
     5341 gaattgaata ccttaggaat acataatagt gggtatagaa tgttagagaa aagaaaatgt
     5401 gctgactata ttcctaatga aaccattacg aaaacagata tggcgctgat gaatcgagaa
     5461 gcagaaaaaa taataaacaa actcacctaa tgaatttcaa gcgagtttgt ttgttatttt
     5521 tttatttaat ctttgtatct tt
//
LOCUS       DAYLZO010000150         5445 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.155, whole genome shotgun sequence.
ACCESSION   DAYLZO010000150 DAYLZO010000000
VERSION     DAYLZO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.155"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..921
                     /locus_tag="ACGVJG_002735"
     CDS             <1..921
                     /locus_tag="ACGVJG_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558637.1"
                     /translation="DDPYWLGKAPLGENIANKSVTFPIKSSAYSLYQANMEEEFVLGQ
                     TYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWGLTFTPQKVGVNYPKRL
                     TIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYT
                     EKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYAT
                     FIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGMLTGPSGTSSVSKAMYV
                     RKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            924..1370
                     /locus_tag="ACGVJG_002736"
     CDS             924..1370
                     /locus_tag="ACGVJG_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558638.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            1372..1509
                     /locus_tag="ACGVJG_002737"
     CDS             1372..1509
                     /locus_tag="ACGVJG_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFJ6558639.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            1546..1839
                     /locus_tag="ACGVJG_002738"
     CDS             1546..1839
                     /locus_tag="ACGVJG_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558640.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            1862..2059
                     /locus_tag="ACGVJG_002739"
     CDS             1862..2059
                     /locus_tag="ACGVJG_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFJ6558641.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            2070..3089
                     /locus_tag="ACGVJG_002740"
     CDS             2070..3089
                     /locus_tag="ACGVJG_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFJ6558642.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            3346..3993
                     /locus_tag="ACGVJG_002741"
     CDS             3346..3993
                     /locus_tag="ACGVJG_002741"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFJ6558643.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            4276..4349
                     /locus_tag="ACGVJG_002742"
     tRNA            4276..4349
                     /locus_tag="ACGVJG_002742"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4310..4312,aa:Ile,seq:cat)
     gene            4895..>5445
                     /locus_tag="ACGVJG_002743"
     CDS             4895..>5445
                     /locus_tag="ACGVJG_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFJ6558644.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNN"
BASE COUNT         1930 a          848 c         1079 g         1588 t
ORIGIN      
        1 gatgaccctt actggctagg taaagcgcct ttgggtgaga atattgctaa taagtctgtt
       61 acgtttccaa tcaaatctag cgcctacagc ctataccaag ctaacatgga agaagaattt
      121 gttttaggtc aaacctatac aattaccatg aaggcaacca aaccacctat ccaaacgttc
      181 attgtttaca acgaggatag tggagattat agatatggta acttagagcc agtagaaggg
      241 ttagttgata catggggtct gacttttaca ccgcagaagg ttggagttaa ctaccccaaa
      301 cggttaacta ttatccagta tccccaatca acaacaggtg catgtcaaat tgactggctc
      361 aagatcgaaa aaggcgacac acgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggcttgaaag acagtaacaa tcccaatgat tacagttggg atgtcacacc tgaatatact
      481 gaaaaaggct tgaatgatgc tgttaatgtg tatgatcctc agagagttga aggtttgaaa
      541 aactttgccg atggtattca aatagcagga gataaagtga tcagtgaaaa tgattgcact
      601 gtttatacat taactaaaga caacagtcaa tcgtttatcg atgggtatgc aacatttatt
      661 aaacacggaa aagaagttat tgtaaatggt acagtaaagt tcaaaaaagc ttatgctttt
      721 ggtgtaccac ttgatgatga agtcccagat gaatttgtcg caaaaattgt tcatggcatg
      781 cttacaggtc cgtcaggaac aagtagtgta tcaaaagcga tgtacgtgcg aaaagactta
      841 ggaacaatta ttacgaatag cgaatttgct gcaaatgaat ggtttacatt ccatggtaat
      901 tattgggtag gggaggaata aaaatgaaaa atatttggaa atacggacga acaggtggag
      961 agtatgctgg acaagtgctt gatgatatgg ttatgactgt tccatttacc gatgttccac
     1021 cactcgaagg aattcgtgct gatggggaac cactaacgat tgctgatcag atgtttgatc
     1081 ctaaactgaa ccaatggatt gttttagtga atgtactaga tcacaacgat ttaaacaatc
     1141 tcaaagcgat gtatgagtcg ttagaaaatg agaacggcga tttaaaacag ctcaacgcca
     1201 aactcatgct aagcgatgta gcgattaaac aggaaaatac tgcattgaaa gaaaaagcgg
     1261 atggtttagc acaaatcaat tcaaaaacga tgcttgcttc gttccaaaat agcaaagaca
     1321 ttgcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag tatgtttagt
     1381 tttagcgatg tgaaaatgat gtatgattgg ggctgtttta ctgacgatca agttcgacta
     1441 ttcgttccac tatgcattac agacgaagaa gcagaaaaaa tcattaataa agataagagc
     1501 gcatcttaag tgatgcgttt ttatttaagg taaaggagtt gtcacatgat taatttagga
     1561 gaatggggaa caatcgcagg atcaatcact gcgattgttt ctttgatttt attagtaata
     1621 agaccagtca ctgcatcttt ctcgaagatt actgaaactc tttcaaaagt aagtcacaat
     1681 ttagatttgc tgactaaaga tttagaatcg agcaaatcag atcgattgat gattcatgaa
     1741 gaactaaaga aacacgatga aagattagat acacatacag aaaaattggt agaacacaca
     1801 caacaaatca aaactttatt tagagaaaga tctcggtaaa aaagaaagga gttaagaaga
     1861 aatgatttta cccgataagt attatcaagt cattaaatgg acagttttaa cagtattgcc
     1921 agctgcatct gtattagttg caacactagg caaagcgtat ggatggaatg gaacagatat
     1981 gacagtactc actatcaatg cagtagcaac atttttaggc gttatcactg gcgtgtcggc
     2041 ttataatttg aaaaaatagg aggaaacaaa tgaaaaagaa aattactatt actgcgatga
     2101 gcctattaac ggctcttttt ttattgccaa ttaatgggtt tgcctatacg attaacaatg
     2161 aatttaattt aggcccaaac gaaggtagct cacaagtagc aaataatcag tacattttac
     2221 tgcatgaaac ggctaatgaa acagcaacag gacgcaatga agcgcagtat atgcaacgtt
     2281 catggactag cgcttatact gcttatattg tgggagacgg cggaattgtt tatcaagtcg
     2341 gtcaacctgg ttatgtacag tacggtgctg gttcgtatgc taatgcaaat agtcctgtgc
     2401 agattgagtt acaacacaca catgataaag caacgtttga gaaaaactac aaggcatacg
     2461 ttgaattagc tagagattca gcaatgaaat atggtattcc acttacacta gacacaccgt
     2521 ataatcaacc agggatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata
     2581 cagatcctta cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta gcctatgatt
     2641 tggctcatgg atttaccgat gaaaatccga caacttcaga tgataaacca gtcattgatc
     2701 caactcgagc aggtgctgca aatcctaccc tgacagatgg aacaaattac gcccacattg
     2761 atcagttcgg agaaatcgaa aacgcaaact tacatgtggc tggttggcac atcgctaact
     2821 ataaatacga gtatatcttc attatggatt acaatactgg gaaagaatta gctcgagtaa
     2881 aagctgatgg aatatataga ccagatgtaa atcaagctta taatacttta ggaaatgttg
     2941 gctatcatgt atcttttaac atgcgcaact tccctaataa gaaagtctat gtaatgatgc
     3001 gggcaacgaa tgatccagaa gggaacacta aaggcggagc acaagatttt catgataaac
     3061 gctggtattt aaatattccg caacgataaa aatagcccct cgtttttgag gggaagtaca
     3121 taatgcaaat ttgtccataa cttattgatt atcttgtaaa aatgcggtaa tttaatagtt
     3181 agtgagaaga ggtgcttaat tatagttaaa aaagaggaaa tagaagcagc atgtaaccaa
     3241 gcaaatgtta ggtttattgg agaagcttta tctggatatc ctaagtataa atacttacac
     3301 aactggttac aaagtaaaag tttttatctt aataaagaaa gatgcatgcc taataaaaat
     3361 tatagggttt ataaaagagg aactattgta tatgttgatt ttggggtgaa cgtgggttat
     3421 gagttatctg gtaatcattt tgctattgtt ttaaataata aagataattc gaaaaatggt
     3481 gttgtatgtg tagtaccaat ctcttcaaaa aagaaaagta attatgtatc tgtaggaaaa
     3541 atattagaag ttgctagtat taaacaattt ataagccaag ctgataaatt aaaagagaaa
     3601 cttaggataa ttattttact ttcattaaat agaggcatca ttgatgaaag cgatttgaat
     3661 agttttatta aagatattga agctaaaggt gaaaagcttt cagtggaaga agtagaaaaa
     3721 cgagctgtaa aacataattt ggtttgcgag acaagggaac aagtaattaa agaaataaaa
     3781 tcattatctg aagatatggt aaaatatcag aaggtgttta attgctacaa gaaatataca
     3841 caagaatcct atgtaatgcc acttaatatc caaacgatta gtaaaaatag aattagaaga
     3901 attaacaagt ttgatccttc tggaaatatg aaggcacctg gaaatgtcct caatgaagta
     3961 gatgaaagtt taaaaaaata ttttacaaag taaacgatat ttttgttgac atttttctgt
     4021 tcgttagtta ccatgtagtt atagaaggac gtaagcgcct atttttaatg tacacatcgg
     4081 gacgtatgcg cccacataga aacatagttg agagacatct atatggtgtc tcttttttta
     4141 ttaatatgca aaaaagtcta gttttttcaa taatatttat gtaataataa attagccacc
     4201 acaacaaaga atgaaaccca ttattatcta gtctatgtcc attctttttg tttgcagtag
     4261 ttgtgatggc tttccgtact cttagctcag ttggttagag cagacggctc ataaccgtcc
     4321 ggtcgtaggc tcgagtccta cagggtacat actctaagtg atttaaattg cgaataaaat
     4381 ataaaaatga ctcccctttt gactccccca agcagtatat ggatgtctct aaaagttgtt
     4441 tttttagata ataaaaagct gataataagc gaaaaacact taaaatcagc tttaaaccct
     4501 attctcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc
     4561 ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata agaacggcca
     4621 aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca gatgcaagct
     4681 tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac agccccttat
     4741 tgttgtactt ttaacctaca atattttttg attccccctg agtactattt tttataaatg
     4801 ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat gtaagataaa
     4861 agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca cttatgcaac
     4921 gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg aagtggtcac
     4981 tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag ataaacatta
     5041 tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg aggctacaaa
     5101 tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt ataaagaatt
     5161 acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac tagggcaatg
     5221 ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca atatcgattt
     5281 tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag aaaaatcgat
     5341 agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg gtgtgttgac
     5401 aaataatccg ccatttgatt atcaattatt taatctgaac aatta
//
LOCUS       DAYLZO010000151         5391 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.156, whole genome shotgun sequence.
ACCESSION   DAYLZO010000151 DAYLZO010000000
VERSION     DAYLZO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.156"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..184
                     /locus_tag="ACGVJG_002744"
     CDS             <1..184
                     /locus_tag="ACGVJG_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558645.1"
                     /translation="KKTMLVLGSISLGVLGFSPMLLSVIGKNNSKGELKLNPNYYNSP
                     NEPMFISEKQNDGQDK"
     gene            complement(203..565)
                     /locus_tag="ACGVJG_002745"
     CDS             complement(203..565)
                     /locus_tag="ACGVJG_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558646.1"
                     /translation="MKKTMLKVFVGLFLLTGIGGIFSKTVDAGSYYYDKSSGNYVINV
                     TNSETGKNLNSIAGKTPGSVTNKMRNAAIKRYGSIQNNRRYYFSYKNIVTPYGGKKGG
                     VSFQWTGRVINMNCTGRS"
     gene            661..1032
                     /locus_tag="ACGVJG_002746"
     CDS             661..1032
                     /locus_tag="ACGVJG_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6558647.1"
                     /translation="MNKNKYYPIYEDLKYKIQHGEYKENEKIPSENQLCNLYSSSRAT
                     IRKAIELLAIFGFVDRSQGKQTIVSSKKNWNKRFLTEEEYKKLVLFNLKSTELNEIIA
                     LSEKYSVLKKLVDFWNNNNLI"
     gene            2138..3070
                     /locus_tag="ACGVJG_002747"
     CDS             2138..3070
                     /locus_tag="ACGVJG_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFJ6558648.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKHEDLRIKLFNIEHNGELTQEEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEGTQKQALSNELKQDLIGNLQNLFD"
     gene            3086..3610
                     /locus_tag="ACGVJG_002748"
     CDS             3086..3610
                     /locus_tag="ACGVJG_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFJ6558649.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSKENRKLWFSKVGNKFVIND
                     IGQKAIKSMFYSENENLNRKLVSGKTKTENLKNANQVSELELVSLLNKQLDQKDKQLQ
                     EKDTQLKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            3986..4267
                     /locus_tag="ACGVJG_002749"
     CDS             3986..4267
                     /locus_tag="ACGVJG_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558650.1"
                     /translation="MDILVRKIDSKIISEIDRRCQELTTKTSKKWSRNTYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNEYVRTNNQLIETLIGGL"
     gene            4269..4685
                     /locus_tag="ACGVJG_002750"
     CDS             4269..4685
                     /locus_tag="ACGVJG_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558651.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISILSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNHQKETNQTFTAGNFDPTNFLKR"
BASE COUNT         2017 a          870 c          868 g         1636 t
ORIGIN      
        1 aaaaaaaact atgttggtac taggttctat ttctctagga gtattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaataattc caaaggggaa cttaaattaa atccaaatta
      121 ctacaatagt cctaatgaac ctatgtttat ttcagaaaag caaaacgacg gtcaggacaa
      181 ataatcctgc cgtcgttttg ctctaacttc taccagtaca attcatgttg attactcttc
      241 cagtccattg gaaacttacg ccaccctttt tacctccata tggcgtaact atgtttttat
      301 aagaaaaata ataacgtcta ttattttgaa ttgaaccgta tcttttaata gcagcatttc
      361 tcattttatt agtcactgat cctggagtct ttccagcaat agaatttaaa ttttttccag
      421 tttcagaatt tgtaacgtta ataacataat tcccactaga tttatcataa taataagaac
      481 cagcatcaac tgtttttgag aaaataccac ctataccagt taatagaaat agaccaacaa
      541 atacttttaa catagttttt ttcatataaa aacctccttt agtttaagaa tatactaata
      601 attataaaga aaacgtttta tattatcaat gttaaaataa taaaaaggag agcttgatct
      661 atgaacaaaa ataaatatta tcctatttat gaagatttaa aatataaaat tcagcatgga
      721 gaatataaag aaaatgaaaa aattcctagt gaaaatcaat tatgtaatct ctactctagt
      781 agtagagcaa ctatcagaaa agctatagaa ctattagcta tattcggatt tgtggataga
      841 tctcagggaa agcaaaccat tgtttcatct aaaaaaaact ggaataaaag atttttaacc
      901 gaagaagaat ataaaaaatt agttctattc aatttaaaaa gtactgaatt aaacgagatc
      961 attgctttat ctgaaaaata ttctgtatta aaaaaacttg ttgatttttg gaataacaat
     1021 aatttaattt aactagcaaa ttttctaaaa agataaaaaa agaagtgggc aatatgggaa
     1081 attaaaccac tacttttttg ttttgttgct ccagcaacgc ttagtcctaa attatccgaa
     1141 agataaaata acaaataaaa tgttcattta ccatataaat ggtcgctaaa ataagcccac
     1201 aaaaaagagc cattaaccct ttgattataa aggattaatg gttcttttat tcttcgaata
     1261 ttttctattt atttgataaa atcacgattt ttttgaattg tgctaccaaa ataaatgcct
     1321 gtatatcaat cattgctacc aaatatttct ctgttattta gcattacgct cccctattag
     1381 agtagggggc acagtatttt ctacctgttt tgggggtttg tcccacatag cattggtgtt
     1441 caagtgctcc gtttttctgt caaaaaggtt cttcctggtt tcatatgttt atgggcctta
     1501 atttgcattt taagttgttt tggtgtctga atcatataag tataccaaag gatgggcaaa
     1561 acagcttaaa tcgaatttta gaaagggttt atgacgttcc agggttgtta gggcttgccc
     1621 tgaccgccta taaggcgctt taagagcaag ataagggttt ttatttaact tgtcgtagaa
     1681 agccttaaac aagcatttag ggaataataa aggccttttt aggaacgggt tgaagcaaag
     1741 gagcctttag tcgactgcgc agtacaatgt gagagcgtag cgatcacacc tttttagcga
     1801 ccgtagggag ccagtttccc agtttaggaa aatgcgcgaa tctgtttgag gagaaaagga
     1861 gtaatttttt aggcgggaaa ttttgcactc cctcttttat aatctctttt taaatctctt
     1921 tttaaacctc tttaccgtcg tctgttccct tactctccca aggggtgtag cttttttagg
     1981 gcatgaaatt atctgtttat aggcatgaaa ttatctgttt ataggcatga aattatctgt
     2041 ttataggcat gaaattatct gttttaggta ttgttttaag acataatatg tcttaaaatt
     2101 atgtcaataa aattagttag actggagtat caaataaatg gcaaatgaaa ttgtaaaata
     2161 tgaaccagaa ttaaatacca ttcctttaag aaaatttacc ccaatagaaa tgaacttgtt
     2221 tttttctatt gtctctagaa tgcgtgataa aggtaatcaa aaaatacgct tttatttcag
     2281 tcagcttaaa gatttaagtg cttataagca aactgctggg gaagtcttta taaaagattt
     2341 aacaagaaca tacgatcatt taatggattt acgttttgga aaaaaaagca atggtggatt
     2401 aaccatagaa cgatttacaa tgtttactga ttttgaaatt catggagaag tcgataaccc
     2461 ttatgtagat ataaaagttc acgaaaaagc tttgccttta cttaacaatt tagaaagttg
     2521 ggtacgtttc tctttaacag aatttcgaga tttaaatagc agttatgcta aaacaatgtt
     2581 tagattgcta aaacaatacc gaactcaagg atgggctgaa ttttccaagg aagatttttt
     2641 agagctatta gatattccta aaagctatag acaaacaaac ataaatcagt ttgtactcaa
     2701 acccataaaa gaagaactaa ctccactgtt taaaggatta acgattcgta aaaaatatgg
     2761 taaaggacga ggaaagcctg ttattggtta tcggtttact tggaaagctg aaattaatca
     2821 tgctgacgat ttctcaaaag gaaaacatga agatttacgt ataaaattat tcaatattga
     2881 gcacaatgga gaattaactc aagaagaaaa atggcgcgct aaagatcgaa tattaaatct
     2941 tcccttaggc actcatgaag cagattttaa aaagcagcaa caaactgaaa aagaaggaac
     3001 acaaaaacaa gcactctcaa acgaactcaa acaagattta ataggaaatt tacaaaactt
     3061 attcgattaa atataaggag tatttatggc agataaaact atcaaagaac ttgctgaaga
     3121 attaggggtt agtaaaacag caatcaataa aaaagtttct aaagaaaatc gaaaactttg
     3181 gttttcaaaa gtcggaaaca agtttgtgat taacgatatc ggtcagaaag ctattaaatc
     3241 aatgttttat tccgaaaacg aaaacttaaa tcgaaaacta gttagcggaa aaacaaaaac
     3301 cgaaaacttg aaaaacgcta accaagtttc cgaattagaa cttgtatctt tattgaataa
     3361 gcaattagac cagaaagaca aacagttaca agaaaaagat actcaactaa aacaaatgca
     3421 aaaattactc gatcagcaac aacaactcac cctgcaagcc aaccaacaaa tcgagaaact
     3481 gcaggaacaa ctacagttga cttatacgga agaatcttcg gaaaataaat caactactct
     3541 ttctgaagaa acagaatcga tagaaaaaca atcaaagacg gagaaaaaat ggtggcaatt
     3601 ctggcaataa aaccagcaat ttttattaat aaaaattgct ggttttattc ttctttttgc
     3661 ttttgcgagc agagcaacat gggcccatgt tgcgatattt tggtagcgcc ccaatccccg
     3721 ctttttcgtt gaatcttact cagactgcac tcgttttata gctgattttt ctgaattttg
     3781 aaagagcata aggaaacgtg gtatcacttt cttacgaaag gtgatagata ggctgtgcct
     3841 atcacatttc taagaaaaaa atttgctatc acttttttgc cattatggat agtgtatgta
     3901 tagtattttg gtaaaaaaca gctatacaaa tactatattt gtatgatata catagattat
     3961 acgcacatgc gaaaggaaaa ttgctatgga tattttagtt cggaagattg attcaaaaat
     4021 tatttcagaa atagatagac gttgccaaga acttacaacc aaaacgagca aaaaatggag
     4081 ccgtaatacg tacctaaaag ttctaatcga aaatgatttt gaccaggcac ttaggcatta
     4141 caaaaaagat caattcgatt tattactcga aaaatttatc gatatacaag caaaaaatac
     4201 ggaaacactg aatgaatatg ttcgtacaaa caaccaactc attgaaacac ttatcggagg
     4261 actataacat gagaaaaact tctgttcaaa aacacgttac cctttatccc agtcacgtgg
     4321 acgttttaga ttccattatt gaaagaaatc cagatatcaa caattactcg gaagccattc
     4381 gttttttatg tatgagttat gaacctgata ccaacacaac cactactagc aaccaagcca
     4441 aactaaatgc tatgggaaaa gaaatttcta tcctgtccga aatcattgca gaatttgctg
     4501 atttatatct aaaagatact ggtgttctta ttggtaccaa ttctaaggtg tatcaagaag
     4561 tgaaagaaaa aattgaaaat caactcaatc cttcccgacg aaatcaaccc aaaaatcatc
     4621 aaaaagaaac caatcaaaca ttcactgctg gaaattttga cccaaccaac tttctaaaaa
     4681 gataaaaaca gaacctgaaa aataaaaaaa ttcctcgatt tctttttagg gggggcggtc
     4741 ttccagcaag atttgcaagt tgggaccaac ttgcggaaaa aaagaaaaat tcgcttgcgc
     4801 gaatccttct ttttgcattt tggggcgctg ccccaatccc caagtatcgg ttatttaata
     4861 gcgtggcaac tcaaagaaca gaacatactc tgaaacaaaa aaagactgct ctaacgagca
     4921 gccagttaaa aaataatgag tgtttattct tgaaaaagca acgcgcttat gaagcgctac
     4981 caaaaattta tcatatttta ttaaaaataa caacccagta aaaaatgact ggtaaaaatt
     5041 cacaaagaaa cggaaagaag caaccgcaga aaatcaagct taaaaagagc tttttaccta
     5101 gaccaatgaa aaatcacaaa gtccgcccct gtacctgttg gtagctgggc tacttggcat
     5161 actgtacatt atgttaactt gaatttaaaa taacatgtat tattgtatta taaagcggac
     5221 tctattgaaa tctataattt tcacttttaa atattacaaa aaatcatata atttgagtta
     5281 ttattgttta gaagtgattt ttacaattta aacatttgac aaactataga acagcactta
     5341 aaatgtgtat acaagtgaca gcgctttaaa aaaggaggtg cagcctatgt t
//
LOCUS       DAYLZO010000152         5309 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.157, whole genome shotgun sequence.
ACCESSION   DAYLZO010000152 DAYLZO010000000
VERSION     DAYLZO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.157"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(71..1219)
                     /gene="nagA"
                     /locus_tag="ACGVJG_002751"
     CDS             complement(71..1219)
                     /gene="nagA"
                     /locus_tag="ACGVJG_002751"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFJ6558652.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1295..2131)
                     /locus_tag="ACGVJG_002752"
     CDS             complement(1295..2131)
                     /locus_tag="ACGVJG_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558653.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2315..3532)
                     /locus_tag="ACGVJG_002753"
     CDS             complement(2315..3532)
                     /locus_tag="ACGVJG_002753"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFJ6558654.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3681..3842)
                     /locus_tag="ACGVJG_002754"
     CDS             complement(3681..3842)
                     /locus_tag="ACGVJG_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558655.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3915..3998
                     /locus_tag="ACGVJG_002755"
     tRNA            3915..3998
                     /locus_tag="ACGVJG_002755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3949..3951,aa:Leu,seq:gag)
     gene            complement(4238..5309)
                     /locus_tag="ACGVJG_002756"
                     /pseudo
     CDS             complement(4238..5309)
                     /locus_tag="ACGVJG_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1736 t
ORIGIN      
        1 ttactagaac acaaaaaaaa ccttaaaacc aaacttaaga agtttagttt taaggttaac
       61 ttaaattata ttatgcgtgg aaacgttcca cgccatctag ataagttgct tccaatgtca
      121 tatcagggtt caatacgata aagtctgcat cgcgtccttg agcgattttt ccacatgtat
      181 catcgatttt acagctgatt gctggaatca gactagccat catgactgct tgttctggtg
      241 ttgctaatcc ccagtctaca acatttttaa ttgcttcttt caatttcaat atacttccag
      301 ctaagttgcc agattctaga cgtgctgttc cttcttttac tactactgga aattcgccta
      361 aattgtaatt tccatcaggc atcccaccgg ccatcataca gtctgtaatt aatgcgacat
      421 gatcatgtcc tgctttttcc attaggattc ccgcagcatt tggatgtaca tgatggccat
      481 cacaaatcaa ttcagaaaag acatgttcta aagataataa cgcacccacc atacctggtt
      541 cgcgatgatt caatccacgc ataccattga aagcatggac aaatacactt gcgcctgctt
      601 caacagcttt ttgcgcttct tctagtgtag cattactgtg tcctagagaa acaactactc
      661 cttcttcagt cacttgtttg acaaactctt ctacaccttg acgttccggt gctaaagcaa
      721 tttttttgat taaaccacct gaagcttctt gccattcatg gaaagtatcc aaatcaggat
      781 ccccgaaata acttgggttt tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa
      841 aatagattcc tttgatttta gctcctggtg cttcctgata cattttgccg attgtctcag
      901 ctacatcttt taaacgttct ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc
      961 cacaagataa cagaccttca gacatcactt tgatgccttc tgcatcatta tccattacat
     1021 catgattcat atagccgtga atatgtgtat ctaccaaacc aggagcaatc catttgccgc
     1081 tttggtcaat gatgttgact tctccctcag gtagttcttt ggtgtattca ccaaaaagtc
     1141 catcggtaat ttcaagatag cctgcatgtt tgacacctga cgctaaaaag aatttgtcag
     1201 catagataaa agttctcatt ctattctctc cctttgtttt tctacctcta aagtactctt
     1261 atttagagaa gaagtcaaga atggtctaca ccatttacaa attttttgaa atttgtttca
     1321 cagcttgtgc taagccttca tagaatgctg ctctttctgg ttcttcttta cgcttgtata
     1381 ttaagctttt aagcacaaat aaatcattga ggtaatagta gctattcttc tcttgagccc
     1441 aatagattcc ttgattcacc tgttctaacg cttcgtcgat ttcattttga tggaataaga
     1501 aatttccata attataaaaa atttgcgaaa attcactttt tgatcgttca tcagctgatt
     1561 gatgcagcag ttggatgctt cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca
     1621 caccatatat ttttccgata caactaatca gttgtatttc tgtgtcagaa acatggattc
     1681 tttctttttg gctagagtag cttaatgctt ctttcaaata ataaagggac tgagagatat
     1741 tttgacgcaa cgtgaattcg caaacaccga gataataata atagcgttga aagtcagttg
     1801 cttgatacag attttcagaa atatcagaag attttagtac ttgttctaac ttccgataat
     1861 cacgtttttg gaaacaacta tccaacagtt caaataattt atcgtttgtc cgagtagttt
     1921 ttcgagagag attcatcaca tgatcaatcg ttacccctag ccgttgacaa atctgcatca
     1981 tcaccaatac attcggcagt tcctcattat tttcgatccg acttaataca ctttgtgaac
     2041 aaatatcatg cgaaagcatt ttttgcgtca ttttttgttc ttttcgaatc tctttaatga
     2101 tcgttccaaa ggactcgatt aacgtttcca taagtatcct ccccaaaata tgcgtattcg
     2161 cattctattc gtattttact ttaggaagtc gcaaacttca agaaaagact tgcaactaaa
     2221 taatatactt tagttattta agaaaactcc actagaaaga gagcctcagt gtgtttgtct
     2281 gtggccctcc tactgaaaaa tttggttttt aaaatcattt ttggcgatag gttgctagga
     2341 attctctcat tttcttagct gtttgtttca aatcatccat tttaggaaga taaacgattc
     2401 gaaaatgatc tggctggttc caattaaatc caccaccatg tacaagtaga atatgatgtt
     2461 catgtaaaaa gtccaaaacg aatttttcat catccaaaat attgaatcgc tttgtatcaa
     2521 tttttgggaa tatataaaaa gcagcttttg gttttacggc tgaaagacca ggaatatcat
     2581 tgattgcatt atagatatat tctctttgtt catagatcct tccgcctggc aggagcagct
     2641 catcgacact ttgataaccg cctagcgcgg tttggataat ttgctgagac aatacattgg
     2701 aacacaaacg catggaggac aacatattca gcccttctat atagcctttg acgtgtgact
     2761 tatctccact taggaccatc cagcctactc gaaatcctgc tacacgatga gatttggata
     2821 gaccgttcag cgtaacaacg aacaaatcag gcgctaatgt agcaatcgga atatgttcta
     2881 gaccatccat cactaagcga tcatagattt cgtcggaata aataatcagg ttattttgtc
     2941 gagctacttc aacgatttct tctaataatt cttttggata aagtgcccca gtaggattat
     3001 taggattaat gataacaata gcttttgtcc gactcgttat ttttgatttg atatcatcaa
     3061 tatcaggata ccattctgcc tcttcatcgc aaatataatg aacaggtgtg cctccagcta
     3121 atgcaataga agccgtccac aacggatagt ccggcattgg aaccagtact tcatctccat
     3181 tgtccagcaa cccctgcata cacatcgtga tcaattcgct cactccgttg ccggtgtaga
     3241 tatcattgat cgtcacattc ggaaaatgtt tcagttggca atattgctca attgcttttc
     3301 tagcagaaaa gatccccttg gaatctgagt agccttctga gtcacgggca ttcatgatca
     3361 aatcacgaat gacttcgttt ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca
     3421 attttaaaat actaatgccc tcttcttgca tccgctccgc ttcttctaga acgggaccgc
     3481 gaacatcgta actgacacct tctaatttgt tggatttttc aaattctctc atcacttagc
     3541 caatccctca tttcaaaaag ttagaacttt ccaaattttt tgttatttac tatttcaata
     3601 tcatactcca tcctagaaaa ttttgccact tatttaggaa aaacgtaagt attaaaaaaa
     3661 cacaaacaag cgttgagaac ttaattgtca tgatagtcat tccatagatt cgctgcccaa
     3721 tccattgcaa ttccacaaat gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca
     3781 agtccaatca atacacctaa gatcattccc agtcctgaaa attctccgtc atatttctcc
     3841 atcttaataa gtcctcccat ttttgagctt gacaactatg taaaacctcg ctatcattag
     3901 tgtgttatta ttacgcagat gtgttggaat tggcagacaa gcatgattga gggtcatgtg
     3961 agcgttagct cgtgtgggtt cgagtcccat catctgcact acaactcgtg aagttaattt
     4021 tgcttcacga gtttttttgt ttgaacgttt ctttatgcca ttatatacat atcactgtat
     4081 cctttacaaa aagaacgtac aatcatgtaa acagcctgag gtgagtatat gaaggtcgga
     4141 aacaagctac tcgtagaaac ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat
     4201 ccctcaccta atactaaagc gagggatttt ttgtatatta tctgtttatc accgttatta
     4261 taaaggtaca aataccaagc acggttaatc caaattgcat aaattcattt ttttgtacac
     4321 tcatgctttt cattagctct ttaactgcca ttagtactaa gatggcgata acaacataaa
     4381 gtatatagta cgtccgcatt ccggaagccc atactgtggg agaaaatccc ataattaacc
     4441 ttgaacaaaa tcccattatc atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa
     4501 aacttaaata aattccaaca attaaacata gtaataatgc caagactact aaagtagcta
     4561 tccaagtacc tggataaaaa agtgacagtt tcgttcctaa ctctgtaaag agattattca
     4621 ggttactact actccaaatc attgcccttt tatttccatt gacgtaagta aagctttgac
     4681 ccattgtatt accaaaataa ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac
     4741 gcacataata gttttgacat ttcctatatg ttagaaggaa aattaagaaa aatagcaata
     4801 aaaataagat attcatgtct aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa
     4861 cttattaaga aagttgaaat ttttatattc aggaaaccat ttattaattt catgtgcagc
     4921 acgtaatgca ttccctggag ttgtcaaaga gaaaattaat tctgctaagc ttattattga
     4981 aaatactgat aacttataat tatatcgtcc attgacaatc aaatataaac taactatact
     5041 tgttagaaca aaaaaacagg cgttgacttg ttcttgatta gttaaaaata tcaataaagg
     5101 aatacttacc caatatattt ttctatttat ttcttctcct cttaagagtt gaaaaaacgg
     5161 ataaaaagtt agtaaactaa atgctactgg ccactgatag ttcaaggtgg ttgctctcca
     5221 accggtttcc cccattactg tcaatggtat taaacagtat atagagcaaa ccaataataa
     5281 atttttaaca tttattttct caaaaacat
//
LOCUS       DAYLZO010000153         5273 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.158, whole genome shotgun sequence.
ACCESSION   DAYLZO010000153 DAYLZO010000000
VERSION     DAYLZO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.158"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(151..2304)
                     /locus_tag="ACGVJG_002757"
     CDS             complement(151..2304)
                     /locus_tag="ACGVJG_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFJ6558656.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="ACGVJG_002758"
     CDS             complement(2598..3446)
                     /locus_tag="ACGVJG_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFJ6558657.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..>5273)
                     /gene="thrS"
                     /locus_tag="ACGVJG_002759"
     CDS             complement(3670..>5273)
                     /gene="thrS"
                     /locus_tag="ACGVJG_002759"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFJ6558658.1"
                     /translation="TAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVE
                     LISELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQR
                     VYGTAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIR
                     RTIERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLR
                     PMNCPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFV
                     RPDQIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLK
                     AAMDELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGE
                     DGENTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAY
                     EIKERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSK
                     ETAVEDLNIFIDAMEAEVKNYSREN"
BASE COUNT         1421 a         1135 c          906 g         1811 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag tta
//
LOCUS       DAYLZO010000154         5206 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.159, whole genome shotgun sequence.
ACCESSION   DAYLZO010000154 DAYLZO010000000
VERSION     DAYLZO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.159"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1367
                     /locus_tag="ACGVJG_002760"
     CDS             <1..1367
                     /locus_tag="ACGVJG_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558659.1"
                     /translation="FYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALT
                     IIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQ
                     IKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSI
                     IQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLF
                     QKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAA
                     KENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGES
                     WEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDV
                     NAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKK
                     NENK"
     gene            1354..2988
                     /locus_tag="ACGVJG_002761"
     CDS             1354..2988
                     /locus_tag="ACGVJG_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFJ6558660.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3163..3996
                     /gene="msrA"
                     /locus_tag="ACGVJG_002762"
     CDS             3163..3996
                     /gene="msrA"
                     /locus_tag="ACGVJG_002762"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFJ6558661.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4076..5065
                     /locus_tag="ACGVJG_002763"
     CDS             4076..5065
                     /locus_tag="ACGVJG_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFJ6558662.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1824 a          806 c          951 g         1625 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttgctc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata
     1381 ttgagtggat taattatagt tagtagtata tattctttca tctttttcaa aaatacacag
     1441 agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact
     1501 atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat
     1561 ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg
     1621 attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat
     1681 cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttag ttgtctctat
     1741 attttctctg cttatcggac agtagacatc tactatcgtt ccgcaattgc cgaagctatc
     1801 gcaataacac ttgttatccc tgtattattt tatgcttatc aaatcatctc tggaaaagaa
     1861 gaaaagtatc cgtctgtaaa gctagcattg agtatgagtt tgctagtgta cagccatgta
     1921 ctatcaacat taatgagtac agcattaatt atcattttta tttttattcg tttggtttct
     1981 aaaggtttca agaatgcaga ttttattgct atatttaaaa aattatttag tgcggcagga
     2041 atgacattgg ttctgacgag tgctttttgg tatccgatgt ttgaacaaat gctctaccaa
     2101 aaaatcaaca aacctagcgt gacaaattta tatgctcatg catctaatgt gttcgatagt
     2161 ctgactgaag ctatgaacaa cgacttaact acttactcaa tgggactagt tggtctgtta
     2221 tctttatgta ttcccctgat tttatttaaa aaattaacaa acatagaaaa aaagatttat
     2281 tatggtacgt gtttgacttg gctagctaca actagtttgg tgccatggta tcttctgcaa
     2341 aatacgccag ccaaattgtt acaatttcct tggcggatct taagcttgca aataatattc
     2401 tcttctctga tattaacgat gatatttttc aaaaatagaa gatacaataa gacaagagaa
     2461 ctattttatt tatttatgac tgttttgtta ttaatcactt taactgtttc tagtaaaaaa
     2521 aatttcaatc aaaaaataat ttctcagccg ggacatattg ttgtaaacaa agaaacagta
     2581 gaggcgtata ccactaaagg aatggattat tttttatatg attattctcc tatcgcttca
     2641 ttggagaata aggagcatat aataaaacac gaagcaaaga ttggcagtca atgggtaaac
     2701 cttccttata aaacaactgg tagttctatc atatatagag taactaatga gaagagccaa
     2761 gtaatcacac ttcctgtata tgcttattta ggaacgaatg tagaaataaa tcatcaacct
     2821 gtaaaggagc aggtgaatgc taatggattg gtacaaataa gcggagtcaa aggagttgct
     2881 gatattaaag taagcacaag ttatacagta acgactatta tttcttatct tgttacatta
     2941 actgcatatt tatgtttatt ttttaaaata gttttccata aaaggtaaat taacttttca
     3001 tttataaaat aaattgatta aaaaggagaa tctgtcgata aggattctct cttttttata
     3061 cgaaaaatct gtagggggat gaagtaatcg cttatttcct cactttatga taaattacag
     3121 ctaaagtcac tagcttgtct tcttcggaaa ggaggagaat ctatggatga ggaacaaata
     3181 ttcattcaaa cactttataa tctgattttg aatccgaata cacgagattg ggaacgaaaa
     3241 gtattgattc agacaaagaa tgacataaga gaaaatatat ccgttaaaga acagttatca
     3301 aaactagaag caacactacg tccattagct atccgaatga atctgacgcc tgatgtaatg
     3361 gacttttatt tattgattac ggaaggattt gataaggagc aaaaatatga cttttccaag
     3421 catgccatgc aagatgctga ctatcaagaa cgtgcagtat ttgcaggggg atgtttctgg
     3481 tgtatggtcg agccgtttga atcgaaaaaa gggatcctat ctgttttgtc aggatacacg
     3541 ggcggacatg ttgaaaaacc aaattatgat caagtaagtg gtggttacac aggtcatgtg
     3601 gaagctgtgg agatcattta tgacacaaga gaaataagtt atagcgagtt gctgacaatc
     3661 tattggcaaa ttactgatcc cacagataca tttggacaat ttcaagatag agggaagcaa
     3721 tatcgtccag tgatttttta tcaaggcgaa cgacaaaaag aattagcgga acaatcaaaa
     3781 cagaaactag acagttcagg aactttccac caaccaatcg tcacaaaaat cgaaccagcc
     3841 ggtacgtttt ggccagcaga aaactatcat cagcaatttt acaaaaaaca accgaaacgt
     3901 tataaaaaaa tccagcaagc acgtaatcaa tttctgatct atcaacgagt aaaaaacaaa
     3961 tggcaaaaaa atatacgaaa aaatcatttt gattaactcc ttttctttaa aatgtagtaa
     4021 actaaaaata gaaaaaagaa agcgttttaa aaagatttga tgaggaggag aaaaattgta
     4081 tattgcagat agtgcacgtt atgaaaaaat ggaatatcgt aaatccggtc attcaggttt
     4141 gaaattaccg attttatctc tcggattatg gcaaaatttt ggtgattatg atcctatcca
     4201 taatcaaaga gagattttaa gaggcgcatt cgacatggga attacccatt tcgaccttgc
     4261 gaataattat ggaggccctg ctggagctgc agagaaaaac tttgggcgta tttttagaga
     4321 agattttcaa gcctaccgtg atgaactgat tatttctagc aaagcaggtt atcatatgtg
     4381 ggaagggcca tatggggaat ggggctctcg taaatccatc atttcaagtt gtgaccaaag
     4441 tctccaaaga atggggctag attatgttga catcttctat catcatcgcc cagaccctga
     4501 tacacctttg gaagaaacag cagaagcttt gatgcaactt gtaagacaag gaaaagcatt
     4561 atatattggt atatccaact ataatggaga agatacaaaa aaaatgactg aaattttaaa
     4621 aagaaaggaa gcaccgttca ttattcatca gatgcgctac aatatgtttt ctcgtgcact
     4681 actagaagac gatctatctc cagtattaga agaagaaggg ctaggggcaa tcacgttcag
     4741 tccgcttgct caagggttgc tgacaaatcg ttatctacat ggtattcctg aagattctag
     4801 agcacatcgt aaggaaatac cattcctttc agaagaacaa gttggatcaa ctctggaaaa
     4861 aatcaaagca ctgcaaacaa tcgccgtatc acgaggacaa tcattagcgc aaatggccct
     4921 tgcatggaat ttgcgacaaa aatctgttac aagtgtactg gtcggagcaa gtcggttaag
     4981 tcagttacaa gaaagtgttc gaatgatgga taatttagat ttctcaccag aagaagaatt
     5041 gagaatcgac caaattctag aatagaaacg atgtaatcaa actttttatc catatattaa
     5101 aaaaaacaaa agatgacatc aagaatcgaa tgaagatcgt ctgttattga acatcatatt
     5161 ttttgtcgtt caaaataaaa atgatcgaag gctaggagtg aatttt
//
LOCUS       DAYLZO010000155         5191 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.160, whole genome shotgun sequence.
ACCESSION   DAYLZO010000155 DAYLZO010000000
VERSION     DAYLZO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.160"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..1592)
                     /locus_tag="ACGVJG_002764"
     CDS             complement(<1..1592)
                     /locus_tag="ACGVJG_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFJ6558663.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYR"
     gene            complement(1704..1913)
                     /locus_tag="ACGVJG_002765"
     CDS             complement(1704..1913)
                     /locus_tag="ACGVJG_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HFJ6558664.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            complement(2902..4029)
                     /locus_tag="ACGVJG_002766"
     CDS             complement(2902..4029)
                     /locus_tag="ACGVJG_002766"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFJ6558665.1"
                     /translation="MSREYYNRIEKGKQPLNDELREIIEKQIERFNPQEPLFLLIDYF
                     RVRFPTTDALAIIRDVLQLKSNYMLYEDYGKYGYESKYVLGDINIMCSMQEHLGVLLE
                     LKGKGCRQMECYLLAQERSWYDFMLDCMTAGGVMKRLDLAINDKAGILDIPKLKEKYK
                     AGECMSYFRMQKDYSGTEKCGSDLPKNTGETLYLGSTSSELYMCAYQKNYEQYVKNGT
                     EIEDTEIKNRFEIRMKNERAYYAVVDLLTYRDAERTAFSIINHYVRFVDREDDKPKSQ
                     WITNDDWAWFVGENREPIRLTTKPEPYTLQKALHWLQRQVAPTIKMVQALDRENRTTI
                     LKDMIEQAELKDKHKHLLQLEKSTIEERIDTAVPQENDGIF"
     gene            complement(4036..4116)
                     /locus_tag="ACGVJG_002767"
                     /pseudo
     CDS             complement(4036..4116)
                     /locus_tag="ACGVJG_002767"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4244..4714)
                     /locus_tag="ACGVJG_002768"
     CDS             complement(4244..4714)
                     /locus_tag="ACGVJG_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFJ6558666.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(4711..4953)
                     /locus_tag="ACGVJG_002769"
     CDS             complement(4711..4953)
                     /locus_tag="ACGVJG_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HFJ6558667.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
BASE COUNT         1422 a         1121 c          775 g         1873 t
ORIGIN      
        1 cggtaatata tctcaatttc ctgtattctt tcgttatcct cattcgttgt tgcttcatgt
       61 atgaggattt tttctatcat ggcatttaat aatggtgctg tcagttcctt tggtacggaa
      121 tattccttga ttaactcaat ccacttttca gcacctatca tatcctgttc cattttactt
      181 agttcttctc ttaggcttgt tatctgctgt tccagttcta tctgttcttt ttggtacttg
      241 ctggacaaca tcacaaaatt tcgctctgtt atcttctcac aggctctatc ttcatacatt
      301 ttcgcaaata aacggtcaat ctcattttgt ctgttctcgg ctttcttcaa tgtacttgcc
      361 tttttcttct tttcccgtat tctctctgca ttgccagctt tctgtatctt gttcaatacc
      421 ttttcttcgt cctgctgtac tgccttagcc caatactgca agcgttccaa tacggcttga
      481 tacagcacat cataacgaat gtagtgcata gaacaattac ctttgccaaa ctgcccgtag
      541 taactgcaag cataataact gtaaggcgtt ttatttgtct tattcgttcc aaatctcata
      601 gaccaaccac aatccgcaca cttgacaagc cctgcaaaaa tcggcgttgc cttttccttt
      661 gtctgtctgc gtcttgattt tatctgctcc tgcacacgat agaacacttc cttgtcaata
      721 atcggctcgt gagtgttttc tactcgaaac cattcacttt cgggcttacg cactttcttt
      781 ttgctcttga acgataccgt agactgcata ttgtgaacac tgtttccgat ataaacctca
      841 cttttcaata tcgccttgac atgagcaatc gtccactcat aacgcttact ttcgggtttt
      901 ccctcaaaga tatgtgcaaa agtaccgtat cttgtaaaat ttagccatga cgctgtcgga
      961 actttttctg ctcttaactg ctttgtgatt tttgcacttc cgtaaccttg ataggctagg
     1021 gaaaagattt tttcaataat ccattttgtt tcatcatcaa ccaacagttt tcctttgatg
     1081 tcagggtgtt tcttatatcc taacggagca taagcaccgt aatgagcccc ctccgcaaac
     1141 tttgtcttaa atgctgattt gactttacgg cttgtatctc ttgccaccca ttcattgatg
     1201 atgttcttaa atggtgcaat ctcactttcg cctttttcgc tgtctacacc gtcatcaatc
     1261 gctatgtagc ggacattatg actagggaaa tacaattctg tatattgtcc tgtcataata
     1321 taatttctgc caagacggga aaggtctttc gttacaacac agttgatttt tcctgcctct
     1381 atatcctcaa tcattctttg aaaactcggt ctgtcaaaat ttgttcccga ccagccgtca
     1441 tcaatatatt catcaatgac attcaaatga tgttcttttg catataaacg caacatactt
     1501 ctctgtgtgg taatgctgga actttcgccc tgtaattcat catctcggct taacctaaga
     1561 taaagtgcag tattgtaaat ctgttgtttc attgtcagtt atcctccttt ataactaacc
     1621 cgtgtttaca atacctgctt gcaagtaaag tataacacgg gtactttttg tcattcaatc
     1681 tatttcttac aactgcaccg attttatgcg attgtctgct ttgctttttc cagcatgaca
     1741 tcagttagaa gttcttccat tgttttgccc ttttcggaaa aatgctcttt tactacaatc
     1801 tttgtctttc ctctgttgcc aatgccacac ttgccatttt tcttttgaat tgtttttaca
     1861 gcattatcaa taacacatac cccctttctg taaaatcttt ctaagcaatt caacggcttt
     1921 tgtcatagcc cacaggagtt ccacctctgc atagttctta tgtagccgta cccattgcgt
     1981 gcgacgctct taatgctcaa gctgtggctg tacgggagta tcattatctg acagtacagg
     2041 tcatggcgat aaaagaggac aaatctttta caagaacaca aataggtttt cgctcgctct
     2101 tgcaggggaa aggtcgtggc gtatttgttt caacggctag ctgtctgtca aacgtattga
     2161 atagctttat tcagttgtca aagaacgatt gaaagaaaaa aatcgtcttt cctatatatc
     2221 gcgttggaaa agaggagaaa cgtaaccaaa atccaaaact ttttccatta ttagtacaaa
     2281 tggaacggtt cattttggca atcaacggtt gattttctcc tttttcacta ctatctgtac
     2341 agcaaacgtc catttgctaa gtttaaatgg aaaaatttta acttcttttc atgcaccata
     2401 tagggataga aaatgtcatt ttgctaagtt gatgatgaaa aaagaatatt ttccttttca
     2461 caccctaact acccaaacca taccgttact gccaacaata gaatgaaata ttttctttgc
     2521 accatatagg gaacggaaac cttaaattgt taagttgaga acaaagtatt ttcatctttt
     2581 cacatcaaca ctcaactgct aatgcctgtt tgaatacaaa atgatgatgt ttttcctctt
     2641 ttcacagaag actgtccact aatcacgaat ttccgtcatt ttttcaaaac ttttttcttc
     2701 tttcacttca tatagggaca gcaagccccg atttaatgac ctattttcaa aaaaagttta
     2761 gatttccttt tctcgcacca tatctgtacg caagtagcca ttttgttgca gatttaataa
     2821 aaaatttatt tttcttcttt cacttcacat agggaatgaa aacagctctt tgctgactaa
     2881 atctgaaatt tttcaattac attaaaaaat accgtcattc tcttgtggaa cagcggtatc
     2941 tatacgttct tctatggttg atttctctaa ttgtaataaa tgtttatgct tgtcttttag
     3001 ttctgcctgc tcaatcatat ctttcagtat cgttgtacga ttttctctgt ctaatgcctg
     3061 taccattttt atggttggtg cgacttgtct ttgtagccaa tgcaacgctt tttgtaaagt
     3121 gtaaggctct ggctttgtgg ttaagcgtat cggctctctg ttttccccta caaaccatgc
     3181 ccaatcatca tttgttatcc attgactttt tggcttgtcg tcctctctgt caacaaagcg
     3241 gacataatga ttgatgatag aaaaagcggt gcgttcagcg tcccgatagg tcagtaaatc
     3301 tacaaccgca taataggctc gttcgttctt catgcgtatt tcaaaacggt tcttaatctc
     3361 cgtatcttca atttctgtgc cattcttgac atactgctca taatttttct gataagcaca
     3421 catatataat tcgctacttg ttgaaccgag atataaggtt tctcctgtat tctttggtaa
     3481 atcgctaccg catttttctg taccgctgta atctttctgc atacggaagt aggacataca
     3541 ttcgccagcc ttgtattttt cctttaactt tggaatatcc aatattcctg ctttatcatt
     3601 gatagccaaa tcaagccgtt tcatcacacc accagccgtc atacaatcca gcatgaagtc
     3661 ataccatgag cgttcctgtg ccagcagata acattccatt tgccgacagc ctttgccttt
     3721 cagttccaat aaaacaccta aatgctcctg catggaacac atgatattga tgtcgccaag
     3781 tacatatttg ctttcatatc cgtattttcc ataatcttca taaagcatgt aattagattt
     3841 cagttgtaat acatcacgga taatcgccaa tgcgtccgta gtaggaaagc ggacacgaaa
     3901 gtaatcaatc aataagaata gtggttcttg cggattgaaa cgctcaatct gtttttctat
     3961 gatttctctt aattcgtcat tcaatggctg ttttcctttt tcaatgcggt tgtaatattc
     4021 acggctgata ccacaggcaa cagcaagcct tgtttgtgtt actccgtatt cctcacgctt
     4081 ttgtcttagt tcttcaataa agtttttatc attcattctt caattcctcc aatcaaaaaa
     4141 ggcaactacc ataattgata attgcctgtc attaaaaatc tgtatagaaa aagcagtcaa
     4201 tcctaagact aactgctttg tgtgtatgga tataaaattg cttctaatct tgaataggta
     4261 tccaaatttc aataactgaa ttatcactat tccaatgaaa acgataatcg tatttttcaa
     4321 agtgtaggat aatatctttt ataggaatat acgatgtact aggaataatt tcttcataaa
     4381 tcttgccata agtttcatat attttatcca tagcaccaat atgctccaca accaaatatt
     4441 tataagacgg tatttcttta tacagaaagc cctctggggt aatggttctt tggggaatag
     4501 aacagaaata ataaagtttt ccatttcttc tttccataaa agcatatttt acccaatttc
     4561 ctgattgtga aagatacgct tttttcaaac tactgttaaa ctttctccaa aactgtgtac
     4621 taatctgaat attctttttt tgataattag tcagttctac ttcctgccca ataactgcaa
     4681 atgcgtttaa ttcctttata gaaaaattta tcattttacc ttgtgagcag tgataacggt
     4741 ataactgtac gcatttaccg ttataataca attatcaatc gttatatacc agttttttcc
     4801 ttttcttgtg atttccgcat tagaaaggct aattttttct ttgcaccatt caattacatt
     4861 ttctacattc aaagataagt tctttttaat tctaataacg ccaagttcag ttgtgtgaag
     4921 tcttcctaag ttttccagta attcattttc catattttga tacctcattt ttcagcaaat
     4981 ttatttgtat gttttatacc ccaattataa cattctcata tcagcactac aataaaaata
     5041 attaagcacc aataatctta ttgaacatct ccaaccgtaa atctttcaca ccatcagtga
     5101 taaacactga gctgacagca attacgacaa ctaccataat tgcctgcact taaatttctg
     5161 caaaagaaaa cgacaatcaa tttcttattt t
//
LOCUS       DAYLZO010000156         5169 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.161, whole genome shotgun sequence.
ACCESSION   DAYLZO010000156 DAYLZO010000000
VERSION     DAYLZO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.161"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..79)
                     /locus_tag="ACGVJG_002770"
     CDS             complement(<1..79)
                     /locus_tag="ACGVJG_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFJ6558668.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFR"
     gene            complement(72..1163)
                     /gene="phnW"
                     /locus_tag="ACGVJG_002771"
     CDS             complement(72..1163)
                     /gene="phnW"
                     /locus_tag="ACGVJG_002771"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFJ6558669.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(1380..1859)
                     /locus_tag="ACGVJG_002772"
     CDS             complement(1380..1859)
                     /locus_tag="ACGVJG_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFJ6558670.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            2218..3000
                     /gene="rpsB"
                     /locus_tag="ACGVJG_002773"
     CDS             2218..3000
                     /gene="rpsB"
                     /locus_tag="ACGVJG_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFJ6558671.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3099..3980
                     /gene="tsf"
                     /locus_tag="ACGVJG_002774"
     CDS             3099..3980
                     /gene="tsf"
                     /locus_tag="ACGVJG_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFJ6558672.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            4116..4838
                     /gene="pyrH"
                     /locus_tag="ACGVJG_002775"
     CDS             4116..4838
                     /gene="pyrH"
                     /locus_tag="ACGVJG_002775"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFJ6558673.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            4841..>5169
                     /locus_tag="ACGVJG_002776"
     CDS             4841..>5169
                     /locus_tag="ACGVJG_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA];
                     GO_process: GO:0002184 - cytoplasmic translational
                     termination [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HFJ6558674.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEER"
BASE COUNT         1745 a          962 c         1072 g         1390 t
ORIGIN      
        1 ttctaaaagc gtggacagga gccatacagc caaagtccac agttgttcct gcccaatcaa
       61 aaattacagt tttaatcatt aacgttctcc ttatagtctt tgataattgc tgtcagttcc
      121 aaaatatctt ccggatagat ctcgccaata tttccgatac gaaaacattc atgatcgctg
      181 attttcccgg gatagatcac aaatccattt tgctttaaat actgataaaa atgattaaaa
      241 tcaaatcgcc agtccttagg gtaaagaaaa gaggtgatga atggcccctg atattcttga
      301 atgaaaggct ggaaccccat ctctgtcatt ttttgtcgta gcacatgatt attggcgaga
      361 taacgtgaat atcgagtttc gattcctcct tctgcctcta gttccttcag cgcttgttga
      421 aaagccaaaa ccgtatgggt aggggaagtg aaacgccatt tgccattctc gttttccatc
      481 acgcggtact ggtcatatag atcaagtgac aaacttcggc taacattttt tcgtttttcc
      541 agttccgctt tattagcaag tacaaaagaa aagcctggaa caccttgaat acatttgttt
      601 gagctggaaa tcaaaaaatc aatccctagt tcggctacac aaatcggtat cccaccaaag
      661 cttgacatcg cgtccactat aaacaaaaga tctctttctt gtgtgacagc agcaatctct
      721 tccaaattat tcaaaatacc ggaagtagtc tcgctatgga ccattgctag tgcggagata
      781 gacggattct cttcaatcag ctgtgtaatc acatgagctt ggaggatttg atgttccgat
      841 acctcataaa cggtatagtt cagtttcaga cattcgcaca tgttgatcat tcgttgtcta
      901 tatgcgccgt ttgaacaaat cagtacagta tcttctggcc tgatcacaga acttaatacc
      961 gattctaccc cgaaggagct gctaccttgc attaagaccg ttgtgtatat tccttccgat
     1021 acttgagcaa gatccaataa tttttttctt atccattgtg taatttcttt gtattcttga
     1081 tcccaagtac aatgatcgat cagcatagca tttttcactg tttcagaagt cgtcaatggt
     1141 ccagaagtta ataatttata catatgattt cctttcattt cttttttgaa atacacaaag
     1201 agcaagaagc tgctcttgtt aacatccaat ataaatgatg attattgtga ttcaatcaag
     1261 gtgttgaaaa atttgaataa aaaatgatgt atttttgtaa agagtgtaaa ggtttattca
     1321 ataaggaaaa tattgaaaat aaaaaaacac gcattccata ttaggaatac gtgctctgat
     1381 taaaaaagtg atacttcagt tggatggtga agaaattctt ctactcgttt tgcgtcctcc
     1441 tcattttccg agatgaccat gatcgtatcg aatccagcaa ttgtactgac aatagaggga
     1501 ggtttgatct catctaaaac agatgcaaat aaatgagcat tgtctggcaa ggtattgacg
     1561 atcgtcaaaa agtggactcg ttcgactttc aatacaatgt cttcaatcat ttgaaccagc
     1621 cgatcctctt cctcagtcct agtctcagga gttccttttt cgcgaaacaa tacgaagcgg
     1681 gaaacgccat tttcatcagg tgccttcacg atttttagat cacggatatc tcgagaaatc
     1741 gttgcctgcg tggcggtaac gccataatgc ccaagtttgt ttaatagttc ttcttgcgta
     1801 cggatcggga attcttcaat gacttgttta atcagcaaat gtctttgagc tttgcgcatt
     1861 ttctttctcc tttacgaaag atttcacatg tatcataaca aaaaaaagaa ggattgccca
     1921 ataatattcg tcttttatac ctaaaattat tttatggaaa aaggataata aagaaacaaa
     1981 gagaaatcta ctgtttttct tgaattcttt ttatctttca ggtaaactat tgaatgttga
     2041 gcagaaaagt atatttgctc ttgcgatacg gatcttttgt ctgtataata gctgagggtg
     2101 ctttatgcat cagcaaatcc acattcttat tggattgtcg gtaggtgctt gaaaaagttt
     2161 gccggcaagg tagaagataa gaagaggaaa aataaaacca aatcggagga aacaaacatg
     2221 gcagtaattt ctatgaaaca attactagaa gccggcgtac actttggtca ccaaactcgt
     2281 cgctggaatc caaaaatgaa gaaatatatc ttcacagaaa gaaacggaat ctacatcatt
     2341 gacttgcaaa aaacagttaa attagtagat gcagcttatg attacatgaa gagcgttgct
     2401 gaagaaggcg gcgtagcatt attcgtaggt acaaaaaaac aagcacaaga agcaatcaaa
     2461 gaagaagcaa ctcgtgcagg acaatacttc gttaaccatc gttggttagg cggaacattg
     2521 acaaactggg atactatcca aaaacgtatc agccgtttga aacaaatcaa tgcaatggaa
     2581 gaagatggaa cttttgaagt tctacctaaa aaagaagttg ctggtttgaa caaagaacgt
     2641 gaacgtcttg aaaaattctt aggcggtatc gctgatatgc caagaatccc agatgtaatg
     2701 tacatcgttg acccacgtaa agaacgtatt gctgttcaag aagcacacaa attaaatatc
     2761 ccaatcgtag ctatggttga tacaaactgc gatccagatg aaatcgacgt agtaatccct
     2821 tcaaacgacg atgcaattcg tgccgttaaa ttgattactt caaaaatggc tgatgctttc
     2881 atcgaaggaa accaaggtga agatcaagta gttgaagaag atttcacaga agaaaacaca
     2941 gcaacatcaa tcgaagaaat cgtagatgtt gttgaaggcg acaattcttc agcagaataa
     3001 ttttgaatga tggagctgtt tcaaaggctg ggcgacaagc ctcactctcc tttgaagcag
     3061 ctttttttaa aaagaaaaac acacaggagg agttaaacat ggcagacgtt acagctaaaa
     3121 tggtaaaaga actacgcgaa atgactggcg taggaatgat ggatgcgaaa aaagcattag
     3181 ttgaagtaga aggcgacatg gaaaaagcag ttgatctttt acgtgaaaaa ggaatggcaa
     3241 aggctgcgaa gaaaaatgac cgcattgcag ctgaaggatt ggcttcagtt gctgtgaaag
     3301 gtaacactgc agcaatcgtt gaagtaaact cagaaacaga ctttgtttct aaaaacgaaa
     3361 tgttccaaga tttagtaaaa gaaattgcag aacttgttgc tgaaaacaaa ccagcagaca
     3421 tggaagctgc aatgaaaatc aaaacagata aaggtacaat tgaatctgac ttgatcgaag
     3481 caactcaagt tatcggagaa aaaatcagct tccgtcgctt tgaagttgta gaaaaagacg
     3541 acaatgctgc atttggtgga tacttgcata tgggcggacg tattgccgta ttgactgttc
     3601 tagatggaac aactgacgaa acagttgcaa gagacgtagc tatgcacgta gcagctatca
     3661 atcctcgtta tgtaaacgaa tcacaaattc cagaagctga attagaacat gaaaaaactg
     3721 ttcttacaga acaagcattg aacgaaggca aaccagcgaa tatcgttgaa aaaatggtag
     3781 aaggacgctt gaagaaattc aaagcagaaa tcgctttggt ggaccaacca ttcgttaaag
     3841 atcctgatat gactgttgaa aaatacgttg catcaaaagg cgcaactgtt aaaacattcg
     3901 tacgtttcga agttggcgaa ggtatcgaaa aacgcgaaga taactttgta gaagaagtta
     3961 tgagccaagt gaaaaaataa ttctgttgaa taacagacgg gaacgcacgg tttgtgcgct
     4021 cccttttttt aagcaaaaca agaaaaacca gtcttagagt gtcgaaatca tctaagtatg
     4081 gtaaaatggc aatagaaaat attggaggaa tgaacatggt cacaccgaaa tatcagcgtg
     4141 tagtcttaaa attgagtgga gaagctttag ctggggacgc aggatttgga atcaaaccac
     4201 cagtaatcaa agaaatcatc caagaaatca aagaagttca cgagctgggc gttgaaatgg
     4261 caatcgttgt gggcggcgga aatatctggc gcggacaaat cggagcacaa atgggcatgg
     4321 aacgtgctca agctgattac atgggtatgt tagctacagt tatgaatgcc ttagctttac
     4381 aggatacgtt ggaaaacgtg ggtgtaccga cacgcgtgca aacttcgatt gaaatgcgtc
     4441 aaatcgcaga gccatatatt cgccgtaaag cagaacgtca tttagaaaaa ggacggatcg
     4501 ttatctttgc aggcggtacc ggcaacccgt acttctcgac agacacaaca gcagcattac
     4561 gcgcggcaga aatcggcgca gatgttatcc tgatggctaa aaacaatgta gacggtgttt
     4621 attctgctga tccgaaagta gatgcaaatg cagtaaaatt tgaagaactg actcatctgg
     4681 aagttattgc caaaggatta caagtcatgg attcaactgc aagttcacta agcatggata
     4741 atgatattcc actgcttgtc ttcaatttaa atgaacacgg aaatattcgc cgtgcaatct
     4801 taggcgaaaa tatcggaaca actgtaaggg ggaaataaaa atggcagatg caattatgac
     4861 agaagcaaaa gaaaaaatga aaaaagcagc acaaaatctt caacgtgaac ttgggcaaat
     4921 ccgcgcaggc cgtgcgaacg ctagcctttt ggaccgtatt actgtgaact actacggtgc
     4981 gccaacacca ttgaaccaaa tggcatcaat ccaaattcca gaagcacgtg ttttaatgat
     5041 cacacctttt gacaagagca ttcttcaaga tgttgaaaaa gctattatgg caagtgatat
     5101 cggaatcagt ccaaccaatg atggaaatgt cattcgctta gttatcccgc aactgacaga
     5161 agaacgcag
//
LOCUS       DAYLZO010000157         5091 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.162, whole genome shotgun sequence.
ACCESSION   DAYLZO010000157 DAYLZO010000000
VERSION     DAYLZO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.162"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..347)
                     /locus_tag="ACGVJG_002777"
     CDS             complement(<1..347)
                     /locus_tag="ACGVJG_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558675.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPA"
     gene            476..799
                     /locus_tag="ACGVJG_002778"
     CDS             476..799
                     /locus_tag="ACGVJG_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558676.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(982..2331)
                     /locus_tag="ACGVJG_002779"
     CDS             complement(982..2331)
                     /locus_tag="ACGVJG_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFJ6558677.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(2400..3353)
                     /locus_tag="ACGVJG_002780"
     CDS             complement(2400..3353)
                     /locus_tag="ACGVJG_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFJ6558678.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(3455..4645)
                     /locus_tag="ACGVJG_002781"
     CDS             complement(3455..4645)
                     /locus_tag="ACGVJG_002781"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFJ6558679.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(4845..>5091)
                     /locus_tag="ACGVJG_002782"
     CDS             complement(4845..>5091)
                     /locus_tag="ACGVJG_002782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558680.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         1495 a         1095 c          860 g         1641 t
ORIGIN      
        1 aaagctggtt ccaaaaatac actctgcttc attgccggca ggtaggtact aggttcagac
       61 aagaccaata ccgtgttgtc tctgaaatgt tgccatgaag caaataaatg cagctccatc
      121 tttggccctg tcatttgggc aaccgtctcc tttgccggaa gtggtaagat caccaaaaaa
      181 tacgcacaca ataaataaaa aacgaaagaa tacaaaataa ctgcccttgt tactagaaat
      241 gttccatact ttcgatattc ataaataaaa aaaacagatg agatcgctaa ggctaaaaat
      301 ggaaaaacaa tcagcgcaaa acgaatcggt tcgatataac ttgccatatg actcctcctt
      361 gattctttgt atctctctta tcttagcagt cctttgctgt ttcaagcaag taacgaggct
      421 attatttctc atttctttca aagtaaagta agataaacac aaagggggtg tcgagatgct
      481 gaatatgtac tttgtttttg gcgtacctat ttttttactg ttcctttatg ctacgatcgc
      541 ttatgtaaga aaaagaacta caattcatta tttaggattt attctcctga ttatttcggg
      601 tttcatgcta gtcttcaatc ttcagacttg gcagcaagca cttcttgaaa tggataaaat
      661 gactccacat gctttgtcta aagtgcttgg ctaccctgtc tatctgattt ggcttcccat
      721 tttcatttct ggttgcttgg ttttattaaa tatttatcgc ggcgtacgcc gaatagttca
      781 gttacgcaaa tcaaagtaaa agacagaaat cggttgagcc cgatttctgt cttttacttt
      841 gctatttcat cgtagacaaa acgacattta cgtgatcaaa attaacgagt acttttgaca
      901 tttcttccac gtactcctca tttaaataaa atattttgtt tggcagagaa gtgcagttct
      961 aataaaattc gaatgtttta gctatctacg ttttaaatag agataaatcg ggataccagc
     1021 aagtgtcaat actatcccca tcgaagctaa aaaggtctga gtgataagcg tgcttgatac
     1081 gataaaagtc cctccaatga ttgctactag tggaataaac ggatacattg gaactttgta
     1141 tggtcgaaac aaatcaggtt cttttttacg aagaagaatg acaccaacaa agaccatcgt
     1201 ataaaaaatc caaatgacaa agatcagcat atccgttagt gtatcaaaac caccgatcat
     1261 catcatgcca atagcgatga ctagctctaa gatacctgcg gctacaggaa ctttagtttt
     1321 atcatgaagt ttagcaaaga gcttgctaaa aggcaggttg ttttcttttg ccataacata
     1381 agggagacgc attccagtaa gggtatagcc attgatggtt ccataaacag aaatcaagat
     1441 accaactgtg accagtcttc caccgaagcc gccaaaaatc atttttgcaa cgtcacttgc
     1501 cgcattgctg tttcctgcga ctccgtcgat agatgcggtt cttaagaaca ccgcatttac
     1561 taataaataa acgatcatga ttccgatgat tcctaaagaa attgcttttg gcaaatcttt
     1621 cgccggcttt ttcaattctc ccgaaatatt tcctacgtgt atccaaccgt cgtatgcgaa
     1681 catcgttgct agcaagcccg ctcccaaggc tgagaaaaat gaaagatttt ctcctgcttg
     1741 gatcggaaac agttggaagt ctactccttc ttgtcggaac aaaccgaaaa tgacgatcac
     1801 aaacaaagga ataagcttgc agacaagcgt aatcgactga aaagcccctc cagctttaga
     1861 acctaaaaaa ttgatcaaca ggatcgatac agcagctgta acagctacgg gaacaatcat
     1921 tgactgggaa agaccaaata gattgacgaa ctgtgtaccg aaaatgatcg acaaggctgc
     1981 tacatttgct ggaaaataaa tcacgacttg cgcccagccc aataaaaaag ctgctgtatt
     2041 tccgtaaatc cgttcgatat atttaatcat ccctcctgtt tctggaatag cagctgccag
     2101 ttcagctcct gttagtcctg cacagacaga aatcatacca ccgagaaacc aagaaaacat
     2161 atgcaaactg gcggatcctg taacttctgc tacgcttgcg gctttaaaaa atacccctgc
     2221 accgatcact gtgcccataa cagtcgataa agcagtaaaa aatcccatcg tacgttttaa
     2281 ttcattttct atttttactt cttttactgg cgtggcttga tattcttcca tcccttgtcc
     2341 cctttcatac catagccaat cccaaaagta taaaaaactt tttggatgtg gttcttgttt
     2401 tataccgtct ctaccaattt ttctttcagt ttttccaaca tatcctttgt catcacatct
     2461 aagtcgtatt tcggcgaaaa gccccattcc tcttgcgcac aagaaacatc tagtgaattt
     2521 ggccaagatt ctgctattga ctgacgtaca ggatccacat cataatccat ttcaaaatct
     2581 ggtatgattt tttgaataga tgctttgatc tcttccgggt caaaagacat cgccgatata
     2641 ttgaaggcat ttcgatgaac caatcgatca ggatctgcat tcatgagttt tataatggca
     2701 tcgatcgcat ctggcatata catcatatcc attgatgtac cttgatcaat gaaactagtg
     2761 tactttcctt ctttgattgc tgaataataa atatcgactg cgtagtctgt cgtaccgcct
     2821 cctggcaagg ttgcataaga aatcaatcca gggaaacgga caccacgagt atctacgcca
     2881 tatttcacat aataataatc acatagtagt tctccagcta ccttcgttac cccatacatg
     2941 gttgtaggac gctgaagagt atcttgtggg gtatgatctt ttggagtatt tggaccaaac
     3001 gcgccgattg aacttggcgt aaaaaatttc agctggaatt ctctagcgac ttccaatgca
     3061 ttgaccagcc cagtcacgtt caaattccag gctaattgtg gttttgcttc tgcaacagct
     3121 gacaaaagag cagccaaatg aatcaatgtg tccacttgat attgttctgc tatgctgcgc
     3181 attttttcgt aatccattac atctaggatc tcaaagatat cgtttttctc aatgacttca
     3241 ggcatgcgta tatctgtcgc aatgacattt tcactgccta gttcttgacg tagccgtgtg
     3301 atcaactctg aaccaatttg tcctaaacat cctgtcacca atactttttt catgatgatc
     3361 ctccctgatc ttttttacta ctatcttcag atgattaaca atatatgcgt gaacatgttc
     3421 tctaatttta aatagacgaa aaaactaaat ttagttaaat aatttccatt tcttttccta
     3481 ttttttgata aactgctact gcttcatcaa gcatttccct cgtatgttcc gcagtcggca
     3541 tatttctgat cctgcctgtc ccaagaggca cagttggata aacgattggt ttagcataaa
     3601 tcccatgttc gatcagcttc ttagagaaag tctgagtcac tttttcgtct cccaaaatga
     3661 ctggagtaat aggtgtttca gacataccga tgttatagcc tacttttttc aattcttctt
     3721 taaaataatt agcattttcc caaacttttt cgacaagttc cggatgttct tgcatcaaag
     3781 taatcgaagc caatgcagct gcagctgcac ctggagttag cgaagtagaa aataggaatg
     3841 gccgacttct agctttcaac cagtcgatta gtgttttcga tccagctaca taacccccaa
     3901 caacacctat agctttcgat aacgtgccca tttgcatatc gattttgtcg cttaatccaa
     3961 aatgcttgac ggttcctgca ccttttccag ttacgcctga gccgtgcgca tcatcgacgt
     4021 aggtgatcag tccatatttt tcaacaatcg gaataatttc cggcaatcgt gcaatgtctc
     4081 catccatgga gaaaacacca tctgtgatat acatgatttt tttatatttc ttactttcta
     4141 tcgcttcctt tgcttttttc tcaaggtctt tcatatcttg atgtttaatt cggatgattt
     4201 ttgctccgga taagcggcaa ccgtcaataa tagatgcatg gtttaattca tccgataaga
     4261 tagcatcttc ttttgtcatt actgcagaga tagcgcccat attgcagttg aatccagatt
     4321 gaaaggcgat tgctgcttct gtccctttga attctgcgat tttttcttct aattgctgat
     4381 ggattttcag cgaaccattg atcgttcgga ctgctcctgc tcctacacca taagtttcgg
     4441 ttgcttcaat acatgctttt ttcaattctt cgcgattggc aaatccaaga taattgttgg
     4501 atgctagatt gatcatcttt tttccgtcta cgatgataca agcaccattt tcactttcta
     4561 gtatgtcgat cgtattgtat aacccctttt ctttcagttg atccaattct ttctctaaat
     4621 acattgttag ttctttggaa gacattgtct ccacctccat attcatctgt cttgcagaac
     4681 ataccaaaca tatatcgtgt cattcatcta caaagcaagt atgctatatt tttgataacg
     4741 cttacactga ttatgagaat tatttaacca cattttttat ttacagtatc tttttacttt
     4801 caaatgactt atatagtatg aacaattacc ttttagataa tgatctattt tactctacct
     4861 gggcagataa atacaacaca accattgata gaagcccttt cgttaaaatc aattgtattc
     4921 tgaatattta atttatttat tgcatagacc aataaaaata cataaaattt ccatcttcgt
     4981 ttatcagcaa tttttaattt tactctctga atatttctta aaagttttac ttgttgaatt
     5041 cgaaaaaagg actgtgacaa aagtcttgtc acagtcctat tcttccgaat a
//
LOCUS       DAYLZO010000158         5024 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.163, whole genome shotgun sequence.
ACCESSION   DAYLZO010000158 DAYLZO010000000
VERSION     DAYLZO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.163"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            282..521
                     /locus_tag="ACGVJG_002783"
     CDS             282..521
                     /locus_tag="ACGVJG_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558681.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="ACGVJG_002784"
     CDS             complement(883..1143)
                     /locus_tag="ACGVJG_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558682.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="ACGVJG_002785"
     CDS             complement(1328..1825)
                     /locus_tag="ACGVJG_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFJ6558683.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="ACGVJG_002786"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="ACGVJG_002786"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFJ6558684.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="ACGVJG_002787"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="ACGVJG_002787"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFJ6558685.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..>5024)
                     /locus_tag="ACGVJG_002788"
     CDS             complement(4547..>5024)
                     /locus_tag="ACGVJG_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFJ6558686.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1529 a         1042 c          771 g         1682 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaa
//
LOCUS       DAYLZO010000159         5015 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.164, whole genome shotgun sequence.
ACCESSION   DAYLZO010000159 DAYLZO010000000
VERSION     DAYLZO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.164"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..620
                     /locus_tag="ACGVJG_002789"
     CDS             <1..620
                     /locus_tag="ACGVJG_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HFJ6558687.1"
                     /translation="VERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEAQAQK
                     VKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAFKEYG
                     EAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNLLSST
                     QETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(708..1310)
                     /locus_tag="ACGVJG_002790"
     CDS             complement(708..1310)
                     /locus_tag="ACGVJG_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFJ6558688.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            1414..2370
                     /locus_tag="ACGVJG_002791"
     CDS             1414..2370
                     /locus_tag="ACGVJG_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFJ6558689.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(2675..3631)
                     /locus_tag="ACGVJG_002792"
     CDS             complement(2675..3631)
                     /locus_tag="ACGVJG_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFJ6558690.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            3805..4365
                     /locus_tag="ACGVJG_002793"
     CDS             3805..4365
                     /locus_tag="ACGVJG_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558691.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            4358..>5015
                     /locus_tag="ACGVJG_002794"
     CDS             4358..>5015
                     /locus_tag="ACGVJG_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFJ6558692.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     N"
BASE COUNT         1658 a          932 c         1043 g         1382 t
ORIGIN      
        1 tcgtagaacg tcaaaaacaa atcgaactgg aagaaaaaga aatcacacgt cgtgaaaagc
       61 aatatgattc ggaagtgaag aaaaaagcag atgccgatcg gtacgctaaa gaacaagaag
      121 cacaagctca aaaagtcaaa gaagtgactg aagccgaagc agaacgattc cgtgtggaag
      181 cactggcaga agcggaagcc aataagacac gtttagctgg acaagcagaa gccgaagcag
      241 cactagcaaa aggaaaagca gaagccgaag caaaacaaaa gatcgccaat gccttcaagg
      301 aatatggcga agcagctgtc ttgagtatgg ttatcgatat gctaccgcaa ttgatgcgcg
      361 aagcagcaca gccgctagga aatatcgaaa aaatctctgt tgtcgataca ggaagcagtt
      421 caggagaaac cagtggagcg aaccgagtga caaattatgc cacaaatttg ctttcttcta
      481 ctcaagaaac actaaaagcg acaacaggac ttgacttgaa ggaaatcgta gagaatttgt
      541 cgacacgtgg gactactagc caagtaaata tgtatgaagc gccaaaagag gcagaacaaa
      601 actcgatgaa ggaagaataa agtagtacac ttcaccgttc aatcaaaaat ggaggaagac
      661 tttttaaagt tcttctctcc atttttttat tttattgcaa aagaaaatta gtcctcgcta
      721 tttttctttt gtttttggat cttagcttct atctcttgta aatttgttct tagcaatcgt
      781 tctttctttt ggagttcgac caaatcttct gatagttctt ccttcttttc tgtttcagga
      841 ggacgaagaa agaaattgat accgattgca atgaatgtcc ctataaaagt atctagtaca
      901 cgttggatag catacaaagc ggattcgcct tgagggatgc tcaacgcaat cagcaacaac
      961 gtagctattg cggaaatgat accggaatta ttgttaatac catctgacac gacgatcaca
     1021 ataataacta gaataggaag caagatcaat tctaccaaaa aatcattgtg aaaatagctt
     1081 ttgacaaaaa aataaatgat agctaatgta ccacctaatg tattgccgat gatcctagac
     1141 tttccaaaag aaacggttgt agtcaagtcc tgacgcaaag aaaaaacagc agataatgca
     1201 gcaatcatcg ggattcctcg ttgagtcaaa tgaaaaaaca agatacataa aaaaacagct
     1261 aaggcacttt tgatcgtacg catacctagt cgaaaacggc caaattgcat agagattctc
     1321 ctttttggtc tttttactat catacctaaa cttttgtctg atagctagtt tttcattatt
     1381 caagtataat taaaataaaa aagggggaag taaatgcgta taggaatgcg aacagtcaaa
     1441 acagtgatca gtgtcttctt ttcgatggtc acagcgggta gcttatcgct gttgtattgg
     1501 ccagctgcag ggatcattgc attattaagt gtaggaaata ccaagcgctc tacattgcgt
     1561 acgggcatga atcgaatcgc agcctttata ttagctacgg caacagcact gttcagtttt
     1621 tttgctgttg ggtatactat ttttggcttc agtctttttt tattcctgtt catcccgctt
     1681 gcctctcgct tcaaattaac cgaagggata gtagtaaatt cggttttggt cactcattat
     1741 ttagtggagg aaaacatgtc ttggcagctg atcggcaatg aagcgggatt aatggcgatt
     1801 ggattagtat ttgctttgct tgcaaatgtt tacatgccag atcgtacgaa gcagctgaag
     1861 gaaaaccaaa tacagatcga aaaagaattt cgaaatattt tgagacaaat ggcagaattt
     1921 cttttatcag aaaataaaga agatgtgcag atcaaatgcg aacatttact gacattcatt
     1981 cgcgaaagcc aagaagatgc cagagaacat caagaaaact actggctcag acagccgcta
     2041 tactacgaaa cttacttctc tatgagaaga gcacaggcca atgtgattaa agacatgctg
     2101 gaaaatctag agagaatcca gcaaccagct tattatggaa aacatatcta tggtttattg
     2161 atttatactg cagaaacatt ttcagagtca aatgatggca gacaaatttt aacacgtatt
     2221 gaagaagttt atgtattgta tcggcaaatg cctctgccga ctagtcgccc tgaattcgag
     2281 gatcgagcag aattgttcca gtttttgcag tcttttaaaa gttttattga aatcaaggca
     2341 gaattctccc aacaaaaaat ccaaaaatag aggttgtgaa ttcagctaat attcaggtca
     2401 aaaagctgat ttcacaacct cttctttaaa cggtgttctc ctgtcaactc ctcggtcaca
     2461 agtcacaata ttcaacaaac atgagaagct gttttttgaa attgctcctt gtgactcgga
     2521 gctgaacggt aggctcacaa cctctttctt aaacggtgtt caaaagcttt cctctgcggt
     2581 aataagtcca gccaaacgaa aaataggagg agctattttt gtttggctgt tcttattact
     2641 tgatgcagaa cagctttttc acaacctttt aaaattacat atttttccat agaacttgca
     2701 tttgatccaa gatttcgatg acatcttttg tcaagaagag agagcgattg ggcaagtcac
     2761 cttcaatcat gtgtaccata ttctttattt cgtaattcat tgcttcagat gtattcccgc
     2821 tttcgatgaa ttcctttgta ccatctgtaa ataagatctc cgcacgatca gcacgcgggt
     2881 aatcagcaat cgtcacataa ccgttctcaa aggcaacgat accgactttt ggcatttttg
     2941 cctgaaaagt caagctgaca gcagcaagtt cattttcttt gttacgcaag atagtgacgg
     3001 attgttcgtc cacgcctgtt tcaaaaggaa ccattgtaga ggcaactact tcaggttgag
     3061 agctcaaaaa gaaacgagcg aaagatacag catacgtacc gatatccaac agagctcctc
     3121 cagctaattc tggattgaag aaccggtttt tagggtctgg ttctttgtaa ctgccgaaag
     3181 gtgcttggat catttttaaa gcccccagtt ttcctgtatc cattattgag cgtaactgct
     3241 gataaagtgg catgttgaag attgtcatcg cttctgctaa gatcaaattc ttttcttctg
     3301 ctaagcgcat agcgtcagcc agttcttttt tattcatcgt gatggctttt tcacatagaa
     3361 cgtgtttgcc tgcttccaat gctgccaaga tatggtcgat atgctgtcta tttggtacag
     3421 caatgtacac gatgtcgatt tcagcatcgt ctaacatttc ttgatagctt ccgtaagctt
     3481 ttggaatatt gtaacggtga gcgaagtttt ccgctttttc tgaagttctg gaagcaactg
     3541 ctgttagttc gctcgtctct tgattgaagc tttcggcaaa ctcatgtgcg atggtaccaa
     3601 gaccaacgat tccccattga tattttttca tctgaaaacg ctccttcata gttggattga
     3661 gtttcttgtc tgtctattat tataacaaaa atcacgaaga ataaagagga gaggacttga
     3721 aacattaaaa aaaatgaaag cagcgattta agaggtgctt tttgatcaaa acttaagtag
     3781 aatagttaaa aggaggggat aaacgtgcat caagtatata ttgatcttat tatgagtgta
     3841 ttattagaac agtttgaaac tgaaaaaaat tttttagatg aatacttgct gatcagtcca
     3901 gaacaatggc aaaaatggaa agatggcgag aaaaatctgc catctgaagc gatgcagaaa
     3961 gtaaaaaatc ttttcactga ttatgaatgg atgctaacgc aaaagatttt gcgtcaaacg
     4021 attatttttc ccgaaaaacg aaacgttgcc gttgcagagt acaagcaaat caaaacacgc
     4081 atcgcgcaaa aatggcttag cagcaaagca gcaaaagtcg agctgatccc tttgaaaaat
     4141 aatcagcagg ctggtagata ccttgacttg aaagtaagta ttcaatatga tgaatggggc
     4201 tatgatgata ttttgaattt tcgtctgcca gcagatgttc aaaaacagat cgaaggagaa
     4261 aaaatcgaac ttctcgattg ggtgaatgaa aatctggaag aaacttacgt tcatgaggcg
     4321 aatggggaac gtgaagaaca acgaaaggat ttttcaagtg aataaatttt taggatttgg
     4381 agcagttgtg attatgatcg cagcaatggg ctatgttatt tttagcgatg atgattcgca
     4441 tgttcaagcc ggcaatcaag cagtcgaacg aacaagcacc aaaacaactg ctagtaaaga
     4501 aaagaacagt caaacaacca gcagcaaggt aaaggacaaa gaaacaaaag atcgtacgtc
     4561 caaacaattg ccagatgtta aaacagacga ttggaagcta gttttggttg gtccagatca
     4621 caaattagaa aaagaaattg atgagtctac tcaattggca tcattaagta acggatactt
     4681 gatcgacaaa cgcattgctc cagaatatga agcatttgcc aaggctgctg aagcagctgg
     4741 atttccatta gtgatggtct cagcttaccg gtctgtatca tcccaacaac aagttttcga
     4801 acaaaatgtc caagatgtga tgagtcgtca aggtgtatca gaagaggagg cgacaaagat
     4861 tactaaagaa acgattacgg agccaggata cagcgaacac cacacaggtc tggcagtcga
     4921 tgtcgtcgat caaaactggt acaacagtta tccttctact gtattggatg caagttacgg
     4981 agatcagcct ggagctaaat ggatcgcaga taatg
//
LOCUS       DAYLZO010000160         4926 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.166, whole genome shotgun sequence.
ACCESSION   DAYLZO010000160 DAYLZO010000000
VERSION     DAYLZO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.166"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            168..1142
                     /locus_tag="ACGVJG_002795"
     CDS             168..1142
                     /locus_tag="ACGVJG_002795"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFJ6558693.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1124..1585
                     /locus_tag="ACGVJG_002796"
     CDS             1124..1585
                     /locus_tag="ACGVJG_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFJ6558694.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1635..2099
                     /locus_tag="ACGVJG_002797"
     CDS             1635..2099
                     /locus_tag="ACGVJG_002797"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFJ6558695.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2113..2862
                     /locus_tag="ACGVJG_002798"
     CDS             2113..2862
                     /locus_tag="ACGVJG_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFJ6558696.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2862..3692
                     /locus_tag="ACGVJG_002799"
     CDS             2862..3692
                     /locus_tag="ACGVJG_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFJ6558697.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3692..4339
                     /locus_tag="ACGVJG_002800"
     CDS             3692..4339
                     /locus_tag="ACGVJG_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFJ6558698.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4437..>4926)
                     /locus_tag="ACGVJG_002801"
     CDS             complement(4437..>4926)
                     /locus_tag="ACGVJG_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6558699.1"
                     /translation="KIRYTVLHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPM
                     GIDNFFASEEKFPTLFEKGSQYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQ
                     CIVGDPLFILEKVGKDKNNQPFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKV
                     ID"
BASE COUNT         1690 a          652 c          906 g         1678 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctggcatttt attatataaa attgttataa
       61 aaaatttttt ttgaaaaata gtattgcaaa ataaagcaat ttcatagata ataggtatcg
      121 taaagttata taactttata atacctatta gagtgaggga taaagaaatg agacaactac
      181 acaaagaaca gattgaaaca gcaatggcag cgttaaagga aaagacaatt gatcatgtat
      241 attttacagc atgtgggggt tcacaagccg ttttaatggc tggtcaatat ctatttgata
      301 aagaattaac tgtaccttgt catgtttata ctgctaacga atttaactat gctataccaa
      361 aaggattatc tgaaaattct ttagttatca cttgttccca ttctggcaca acaccagaaa
      421 ctgtagaagc aacaaaaaaa gcaagagaat ttggagcaat gactatttgt ttatctaatg
      481 aagaaggatc tcctctatgg gaagcagctg aatatcctgt tcactatgat tggggtaaag
      541 atgttgatgc tagtgattta aataaaggaa ttctttatgg tttattattt aatattttac
      601 ttgttttaga tggctctaaa aaatgggaaa cttgcttgaa tgaatttgat aagttaaatg
      661 atcttgttat tgctgaaaag agcaaatttg aaataactgc taaagaatgg ggtaaaaacc
      721 aaaaacgaga taaaattatt tacacaattg gtagcggtat taattacgga gaaatttact
      781 ccacagcagc ttgctggttc atggaaatgc aatggattaa ttctaatgct attcattctg
      841 gggaattttt ccatggtcct tttgaaatca ctgattatga tgtaccattc atcttagtga
      901 aaagtattgg agatacaaga tttttagatc aacgggtaga agacttcact aaaaaattca
      961 gtaataaatt aatggtttta gaccaagctg attttgactt agaatctata gatagtgtag
     1021 cgaaagaata tattgcagct gtacttagtg gcgttgttat tcgccaaatg gtagaagcga
     1081 ttgcttttga aagaggccat tccttagatg ttagacgcta tatgtggcaa atgaaatatt
     1141 aattaatagg aggttgcaaa agatggcaaa tcaattattt cacattgtga ttacaacaca
     1201 ttctaccctt tgtgagggtt atttaaaggc aacaaaatta attttaatga atgacgccac
     1261 aggtattagt gctgttccat ttaaagaaaa tactagtcca gatgattttg aggagagtat
     1321 gaaagaaata attagtcagc atcatttgca acctctaatt attttaacgg atttaattgg
     1381 aggaacacct aataatgttt ctattaaaca gttgaacaac aatagaattc aaattattgc
     1441 tgggattaat ttacctcttt tactggagtt attaatggcc cagtcagctg gtaaatctat
     1501 agaagaaata gattttaagg aattactgaa taactctcaa aaaagtatgg ttcatattaa
     1561 ttcgctttta aaagatgtca gttaaggtgg caatttctat tctataaatt ttaaaaattg
     1621 aaggaggaaa tagaatgatt aaattagtgc gtgtagacca cagattactt catggacaag
     1681 taatattttc ttggactaaa cagtatgata ttaaccatat tattgtggct gataacaaag
     1741 ttccaggaga tccaatgtct gtgatggctt tgacaattgc taaacctgct gattgcaaat
     1801 tagacattgt taaaattagt gatgttaaag ggattatgga aacatcccca aatgataatt
     1861 acatgatttt aattaaagga cctaaggaag cttttgaatt agttcaaaat ttaccagaaa
     1921 ttaaagaaat taattttggc ggtgtgtcta agaaaagcga gtccaaacaa tatggaaagg
     1981 cagtcttttt aactcctgaa gaattagaag ccacaaaaaa aattcttgat caagaagtga
     2041 aaatatttgt gcaacaagtc ccatcttcaa gtattgaaaa tgtagatttt agtaagtaag
     2101 gggggaaaat aaatgttatt aaaagccatt ttattaggtt tagttggtgt ggtagctgtt
     2161 attgactcca ggttaattgg tcgacaaaat attggtagac cactaatttt gagtacactt
     2221 gccggacttg tgctaggtga cttgcataca gggattgttc ttggggcatc tttagaatta
     2281 atttctatgg gattcgtctc aattggggcg gctggacctc ctaatatgca acttggtagt
     2341 attattgcca ctgcttttgc aattttaact aatagtagta cagaaacagc cttaacaata
     2401 gctatcccag tggctgtagc tggtgaattt atgagtatta tcatgcgtat gataattgct
     2461 caatttggac atgctgcaga taaagccatt gaaaataata aatttagaaa agctcaaatt
     2521 attcatattt attggtcatt tatttttaat gcttttgttt actttattcc aatcttttta
     2581 acagtatatt ttggtgctga cgttgtggca gatttagtag ataaaattcc agaaatgatt
     2641 acagacgcct tgactgtctc tggaaatatg ctttccgcat tagggtttgc aatgctatta
     2701 agcacaatgt tgagtaaaaa attatatcct tactttattt ttggattctt tattgttgcc
     2761 tattcaggtc tttcactaat tggcgtgaca atttttgcag ctctgattgc ctttgtaatg
     2821 gatcaagtta aatatggaaa aagagaagag gcgcatggtt aatggaaaat agtacagaaa
     2881 aaaaacgttt aatcacaaaa aaagatcttc ataagatgtg tcttcgttca tggacaatca
     2941 tgtgttcgtg gaattatgaa agacaaatgc atatgggatt tatgtatggg atggcgccaa
     3001 ttttaaataa agtttataaa aatgatgaag agaaaaaaag agaagcttat actcgtcata
     3061 tggcattttt taactgtacg cctcaaatga ctccatttat tatgggactt tgtgcttcta
     3121 tggaagaaca aaatgctcaa gcagaagaag gaaactttaa tactgaatct atctccatga
     3181 ttaaaaccag tttaatgggt ccttttgctg gaattggtga ttcatttttc caaggtactt
     3241 taagaattat tacatttggg attggattgt cttttgctaa gcaaggtagt attttaggac
     3301 cgattttagc tgttggcctt tttgctatac cttctatttt agctgttttt tacggcggta
     3361 aattaggata tgagtctggc aataaatacc ttactaaatt gtatcaagaa ggtcttatgg
     3421 atcgagtaat gcaatttgct tctatcattg gtttggctgt agtaggtgga atggtggcta
     3481 gcatggtgcc aattgtcacc cctttaaaat tcactacagg gggaacagaa cttgttttac
     3541 aagacttact tgattccatt attcctggat tattaccact tgtatgtact ttgggaatat
     3601 ataaattgat taagaaaaat gttagtacaa atatgctctt attaggttgt attttggcag
     3661 ggtttgtttt aagtataata ggtatcttat aatgattaag gctctaattt ttgatatgga
     3721 cggagtttta attgatagcg agattatgta tcaaaagtgg gtatttaatt ttttaaaaca
     3781 agaaaattta agctatcctt tgtattctta tcaaaaaaag attggaacta cagcaagtat
     3841 ttttgatgat atagaaaagt acaatgaggg atgtaacagt ttagaattga agggaaaatt
     3901 tactaaatat tggaaatcgc aaaagattga ttacttggat atttttccta atatactaat
     3961 tgaacagctt gaatggttaa aaaagaaaaa aataaaaatg gcaattgctt cttccagtcc
     4021 aatggatact ataaatgaag ttgttaccac ttgtaaaata gagtcatatt ttgattgttt
     4081 aattagcgga agagatttgc cagaaagtaa acctaatcct actatattcc ttaaagcttc
     4141 agaacagctc agaattccag ttgaagaatg tctggtgata gaagattctt ataatggtat
     4201 aaaagctggt aaaagagcaa atatgaaggt tcttgcaatt aaagataaac gtttttctca
     4261 agatgtaagt ctagcagatg atgttattta cgatattaaa tatctgcgac aagaagtaca
     4321 ggtgaaattt ggaatataaa taagtttttt gatgtattta gagaatatta gtaaaaataa
     4381 aaatcatgcc caaatctaat caaaattgat tgctttgggt atgatttctt ttaatatcaa
     4441 tctattactt taggatctga atcattttta tgcaccgtat ctaaagtgat attttctgtg
     4501 acttcaataa cataactaat tctatcacaa cgaactgtgg attgagaata atgaaatggt
     4561 tgattatttt tatctttgcc aaccttttct aaaataaata gcggatctcc tactatacat
     4621 tgcaataagt cggaaagctc aggtgttgca gataaaccgt ttatttccat aatagagctc
     4681 gtagctttga catcatagtc atcttctaga agtttatata atgaagtata ttggctacct
     4741 ttttcaaata aagttggaaa tttttcttct gatgcgaaaa aattatcaat tcccatcggg
     4801 gcaccgtctt cccacattaa acgtttaata ttgtaaactc tatcagttac attgatttgt
     4861 aatttattag ctaaaaactc atcagccttt ataattttag aatgtaaaac agtatatcta
     4921 atttta
//
LOCUS       DAYLZO010000161         4913 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.167, whole genome shotgun sequence.
ACCESSION   DAYLZO010000161 DAYLZO010000000
VERSION     DAYLZO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.167"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            85..972
                     /locus_tag="ACGVJG_002802"
     CDS             85..972
                     /locus_tag="ACGVJG_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFJ6558700.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACGVJG_002803"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACGVJG_002803"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFJ6558701.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACGVJG_002804"
     CDS             2274..2720
                     /locus_tag="ACGVJG_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFJ6558702.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACGVJG_002805"
     CDS             2993..4345
                     /locus_tag="ACGVJG_002805"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFJ6558703.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACGVJG_002806"
     CDS             4516..4803
                     /locus_tag="ACGVJG_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFJ6558704.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          807 c         1029 g         1384 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aag
//
LOCUS       DAYLZO010000162         4912 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.168, whole genome shotgun sequence.
ACCESSION   DAYLZO010000162 DAYLZO010000000
VERSION     DAYLZO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.168"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(560..1600)
                     /locus_tag="ACGVJG_002807"
     CDS             complement(560..1600)
                     /locus_tag="ACGVJG_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFJ6558705.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="ACGVJG_002808"
     CDS             complement(1949..2275)
                     /locus_tag="ACGVJG_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558706.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="ACGVJG_002809"
     CDS             complement(2262..3050)
                     /locus_tag="ACGVJG_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6558707.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="ACGVJG_002810"
     CDS             complement(3418..3708)
                     /locus_tag="ACGVJG_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFJ6558708.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="ACGVJG_002811"
     CDS             complement(3844..4023)
                     /locus_tag="ACGVJG_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558709.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="ACGVJG_002812"
     CDS             complement(4020..4370)
                     /locus_tag="ACGVJG_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558710.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..>4912)
                     /locus_tag="ACGVJG_002813"
     CDS             complement(4363..>4912)
                     /locus_tag="ACGVJG_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFJ6558711.1"
                     /translation="IAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRI
                     RQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVR
                     HVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSMLEGA
                     RSITRSTLVGGHAGGNGGIKND"
BASE COUNT         1470 a          868 c          704 g         1870 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta ta
//
LOCUS       DAYLZO010000163         4912 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.169, whole genome shotgun sequence.
ACCESSION   DAYLZO010000163 DAYLZO010000000
VERSION     DAYLZO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.169"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACGVJG_002814"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACGVJG_002814"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFJ6558712.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="ACGVJG_002815"
     CDS             complement(1098..2201)
                     /locus_tag="ACGVJG_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFJ6558713.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACGVJG_002816"
     CDS             complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACGVJG_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFJ6558714.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..>4912
                     /locus_tag="ACGVJG_002817"
     CDS             3528..>4912
                     /locus_tag="ACGVJG_002817"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFJ6558715.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFG"
BASE COUNT         1436 a          967 c          945 g         1564 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt ga
//
LOCUS       DAYLZO010000164         4864 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.170, whole genome shotgun sequence.
ACCESSION   DAYLZO010000164 DAYLZO010000000
VERSION     DAYLZO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.170"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..73)
                     /locus_tag="ACGVJG_002818"
     CDS             complement(<1..73)
                     /locus_tag="ACGVJG_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFJ6558716.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDY"
     gene            complement(692..1024)
                     /gene="traE"
                     /locus_tag="ACGVJG_002819"
     CDS             complement(692..1024)
                     /gene="traE"
                     /locus_tag="ACGVJG_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFJ6558717.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(1861..2946)
                     /locus_tag="ACGVJG_002820"
     CDS             complement(1861..2946)
                     /locus_tag="ACGVJG_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFJ6558718.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(3266..3673)
                     /locus_tag="ACGVJG_002821"
     CDS             complement(3266..3673)
                     /locus_tag="ACGVJG_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558719.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(3692..4510)
                     /locus_tag="ACGVJG_002822"
     CDS             complement(3692..4510)
                     /locus_tag="ACGVJG_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFJ6558720.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT         1476 a          832 c          678 g         1878 t
ORIGIN      
        1 tgtaatccca acgttggaaa gaaaccttaa tagtatgtgt gcccaatcgt atattcggat
       61 cataattttt catttgtcat tcctccatta cgtaaatttc attttctcta tgttttatat
      121 caattttttc actttattca ggactcgttc atattttatc gtttttcgtt tacttaaaaa
      181 tacataaaaa atatcgactc gtttatggcg tccgaaacga gaaaccctag tagaaatcgg
      241 taaatgctcg attgatccaa tgaccaacgt cctcgcaagt atctacatgt actgcagcac
      301 acgacacaat ttcttgtgcg tcctccccat tacgagaaga cctactcctt attccatgag
      361 tttgcgtcaa agcctagatc ccttagtagc tgctcatgat taatctaggc gctattctaa
      421 cttgtctaac ttctaaattc cctcttgttt actctgtgca tattatttac acaaattcat
      481 ttcaccacta tccaaacaag ggcatttact cgattgacca attttttacg ttaccaatcg
      541 taaaaaccta ccccgtcact ccccgaaggg aattgttgat agatcaagtc tactccttat
      601 ttttctagtg attctaagtt cctttagaag tctcgacaat attttcaagc aagcgaaaaa
      661 cgataaaata caagttatga gaatgatcaa ttcagctgat taaacgcccc atcccttttg
      721 cggtacaacg caagactcca ttttttcttc gaccaatcag tacaccgttt tggtcataaa
      781 catgtttgat tgttggatat agctctttta aagggatagg atcttttaaa attatttgtt
      841 tttgtttccc aatgtaataa atcatttctt ctgagacttt gtctttctgg attatatatt
      901 tcttacactg cacaacggtt ttactttgac cagttgatag tttcttcgtg atcttttctg
      961 tagcggtaca ccgtttaccg tagtgacaaa tatcttcgag actcaaaatt cctgtatatg
     1021 acataaaata atccacccac ttactttaaa tatttttaac aagtttttct tgttattttt
     1081 caaaaataga gaattgattt tcgttcatga caaacaaaaa agagggaacg cattcaaaca
     1141 tgcgttccct ctaatttcag cgttcgtcat acggcaaaga gccccacgga catttttata
     1201 acattatgct taggcataga ttcatcgtaa tatcacgtat ttttaacctt aacacagcca
     1261 aaaaaagtgg tatcactttt gaatatctgt tatactaaat ccgttacggg taataccgta
     1321 acactgaaag gtaaacgcat taaggggtat agacttaatg ccacctgtgg acgtcgacga
     1381 aggttggtag ccttccgagt ctcacagggc ctttcttttt tttgtatttt caattgtcca
     1441 aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc acaagcatag cagacaatta
     1501 aatttaaaac aactaaattt atacaaaaaa gtgtaattga atattagcag cactgcctga
     1561 aaagaagtaa tatcaggctt ttcagcttgg gtaaataagt gaattacctc tcttcatgaa
     1621 ttattataac tcaaaaaaat acatatgcaa acactagttc cctgtttttt tgaatgaaaa
     1681 ataactaaaa tatctaacga ttatgacatt aaaacctacg tttgtgattg ttttcataaa
     1741 cttttaatct aaaaactccg gaataataac tatttatttg taacatttgt aataaaatta
     1801 aaattgtaaa aaagttctaa tttaaaattg atcattttta tagtgttcgc tttttatcaa
     1861 ctactctagc caattttccg tactaactgt tggtaattcg ttaaatgttt ctctttgttc
     1921 gtttgcaagc tttgccgcaa tagctttttc aataaaccag ttttttacat acacaaaaat
     1981 caaattctgc aaattattta ctttctcagt cttttgtttt tgataagctt tcagcaacgt
     2041 catatataat cctttatccg catcaattcc gtaagattca agttcttcat acacaactac
     2101 cgtcttctca agttcctcgg ctttatgttt tgctttgtgt attgtggtga cggtttgttg
     2161 ggcttcttcg taagtgttag aaaatgtttt tatcaagctt aatacctttt ctggtacaaa
     2221 tgtgtttatg ctgtcttctt tcatgatttc aacgaagtta tctagcaaaa cttgattttg
     2281 taattggtct gtttcagtgt cttttaagtg tcttttagtg tctaaagatt tattaatata
     2341 ataatcttgt gcgatttccg catttccagt gttatcaagg gtttcagctg ttttttctcg
     2401 acgtgcgatt tccgcatttc cagtgttatc aagggtttta gcagtttcct ctcgacgtgc
     2461 gatttccgca ttccgagaag aggttgattt atcaatagtt tcgcttaaat cgtcatcgaa
     2521 agaattttga ataaaaagat catcggcact tatttcaggt tgaagtaaat agtatttatt
     2581 tttttcattt tttttcgttt ttgggttaaa ttctcctttt acgactgaaa tcaaattata
     2641 tgtttctaac tcgtttaaaa tggtttttac tttgccttgg tagcaattta atttgtccat
     2701 taggtctgct tgtgtaaatt ccaaatataa tagaccatca tcgtcgatcc atttttcatg
     2761 taaggctcta cgaaattcat ttttaaaaag catataggct atttttgagc tgtctttcat
     2821 tttagagtat ttagaattct gaattaaaaa actaggaaat ttataatatt tttcaatata
     2881 aacattcgtt gctttatatc cccaattttt aattatcatt ttttgagatt gattttctgt
     2941 agacattaaa tttctccttc ttcttagaaa cttgtatata ttcaggattt tcaacaagtg
     3001 aactatacca tgtattaagg gtgaataaaa cactttcagt atttaaggta taagttatat
     3061 attaaaaaaa taagtataag ttatacttta aatacaaata atgtataact tatactttaa
     3121 ataataattt tataatgttg atttaatcgc aaatttacac gttgtttatt aagttatacc
     3181 ttaaatattt aatgtataac ttatacttta atattcattt atctagtata acttatactt
     3241 taattgaata ccgcatgaaa aaaaattatt ttggtaattt ttcaaactct tcttgtattt
     3301 cttctcttat gtgttcttct tttgctactc ctagtctatg ctggatatat gagtctgcta
     3361 atgtattaac aattttttcg aaagaaattc tccctttttc actttgtaac tccgtcatat
     3421 agtcttgtaa aattgagagt ttgctattta atgccggaga gatcttttta ggaactgata
     3481 ccttgtttct tttatcagga attgaaactg gtcggcctct ttttttcaat gtaatatttc
     3541 ttgtgttact caatgcacta ttgctaaagt tatattcacc gcttattttc acatcttggc
     3601 gtttgttatt gggtaataat ggctcaaatt caccattctc catcatttta ggaatatcaa
     3661 aatctttagg cattacttat gcaactcctt attatttatt taaaaactct tgtctagcat
     3721 ctagttctgc caaaatattt ataaatactt ggtgtgcttt ataatcccat tgatcaaaat
     3781 ttccattttt gtattttctt aaatgtatac caattttaga ataccttctc aatctttttt
     3841 gataatgaat gagatttttg gataccacgg ttccgtattt ttcaattgct tcttctagga
     3901 tttcttcatc aaaatcatca tttggttcta ccatacaagg tacaatatct ataatttgca
     3961 aatcaagatc gtatctatcg accatgaatt tttgataatt tacatatttg ttgactccct
     4021 ctaaggattc ttcagctgtt tgaaatgaaa ttatcgaata gtctgaagct gccatagcat
     4081 tatcggaaaa ttcgctaatg gttggtggta catcaataaa tatttcttga taattgttct
     4141 ttattggaag aagtaatttt ttcaagacag taacttggtc taattcattt tcatgtttat
     4201 tagcaaaagc ggagaatgat cgaaaactag tgtcacatgc cagaaggtca agattttcat
     4261 ttaccttaat aatactactt gctaattctc catttgcgac accatttgtt atagtggttt
     4321 tgatatcggt tataccgaaa gttcttgcta tgacttgtgt taagttcgct tgagggtcta
     4381 aatcaataat aagcgttttt ctatttctta tcattgcatt gtcataagca agcatgctga
     4441 ctatcttagt tttacctact cctcctttaa aattgccaac tgtaaatgta gtcccttgaa
     4501 aagtatccat gtaaatcctc ccaatcttta attgataatt aaagtataac ttatatatcg
     4561 gaaatggtca acattaaaag tataacttaa ataataatat ctaaaaacgt ttggatatag
     4621 cggtttttaa taataaaagt ataagttata ccatatatat ttatggtata acttatactt
     4681 ttattattaa aggtataact tataccttaa atattgcttt tcgaaaatag ctattctagt
     4741 ctatctgttt ctttactttt cgctgctgat ttgaattgaa ttgatcatat tcattttcta
     4801 gtattaataa cttttcgtat acatcggtca tattcatact agaagcaaga cttagatcat
     4861 atac
//
LOCUS       DAYLZO010000165         4807 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.171, whole genome shotgun sequence.
ACCESSION   DAYLZO010000165 DAYLZO010000000
VERSION     DAYLZO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.171"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="ACGVJG_002823"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="ACGVJG_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFJ6558721.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1523..1718
                     /locus_tag="ACGVJG_002824"
                     /pseudo
     CDS             1523..1718
                     /locus_tag="ACGVJG_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3250..3768)
                     /locus_tag="ACGVJG_002825"
     CDS             complement(3250..3768)
                     /locus_tag="ACGVJG_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFJ6558722.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4135..4383
                     /locus_tag="ACGVJG_002826"
     CDS             4135..4383
                     /locus_tag="ACGVJG_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558723.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1579 a          786 c          729 g         1713 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DAYLZO010000166         4747 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.172, whole genome shotgun sequence.
ACCESSION   DAYLZO010000166 DAYLZO010000000
VERSION     DAYLZO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.172"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(451..1761)
                     /locus_tag="ACGVJG_002827"
     CDS             complement(451..1761)
                     /locus_tag="ACGVJG_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFJ6558724.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACGVJG_002828"
     CDS             complement(1773..3191)
                     /locus_tag="ACGVJG_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFJ6558725.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..>4747)
                     /locus_tag="ACGVJG_002829"
     CDS             complement(3431..>4747)
                     /locus_tag="ACGVJG_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFJ6558726.1"
                     /translation="FYLVYTDVKSAGRPFKDVHNYLITASEITGPWSEPIYLNSNGFD
                     PSLFHDLETGKKWLVNELWDYRLDTPNKSSGIVLQEYDAEKKKLDGPIYKIFAGTELA
                     KTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVCRAKNITGPYELDPGFPMLTASDKPAS
                     TLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAAILGRETAIQEVYWTSDDWLRLKDSQT
                     TPMEEIMIETNEEVQQEKDHQFMDTFEGELKKNWNARRILPNADWCDTKCRPGFLRLI
                     SGESLQSTFEHHLLAIRQTDFTFDAETAFEYMPNNFNQMAGLVLYLNESNYIYCYVTW
                     NEDKGKCLRMIESENGVAQIEDWTIPLTEKKTFLKVTVKKETAQFHLKDVSTEEWKKV
                     GPQKNMCILSGGFTGNFIGIAVHDLNKKRGSYADFEFFKYDGKDHL"
BASE COUNT         1446 a          979 c          738 g         1584 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaa
//
LOCUS       DAYLZO010000167         4741 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.173, whole genome shotgun sequence.
ACCESSION   DAYLZO010000167 DAYLZO010000000
VERSION     DAYLZO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.173"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(25..1023)
                     /locus_tag="ACGVJG_002830"
     CDS             complement(25..1023)
                     /locus_tag="ACGVJG_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFJ6558727.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1098..1394)
                     /locus_tag="ACGVJG_002831"
     CDS             complement(1098..1394)
                     /locus_tag="ACGVJG_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558728.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1387..1680)
                     /locus_tag="ACGVJG_002832"
     CDS             complement(1387..1680)
                     /locus_tag="ACGVJG_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFJ6558729.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1826..2377)
                     /locus_tag="ACGVJG_002833"
     CDS             complement(1826..2377)
                     /locus_tag="ACGVJG_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFJ6558730.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2551..2850)
                     /locus_tag="ACGVJG_002834"
     CDS             complement(2551..2850)
                     /locus_tag="ACGVJG_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFJ6558731.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2877..3152)
                     /locus_tag="ACGVJG_002835"
                     /pseudo
     CDS             complement(2877..3152)
                     /locus_tag="ACGVJG_002835"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            3490..4173
                     /locus_tag="ACGVJG_002836"
     CDS             3490..4173
                     /locus_tag="ACGVJG_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFJ6558732.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(4210..4707)
                     /locus_tag="ACGVJG_002837"
     CDS             complement(4210..4707)
                     /locus_tag="ACGVJG_002837"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6558733.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1404 a          907 c          694 g         1736 t
ORIGIN      
        1 ttctttgata aataatattt tcaattattt aatcatttga aaattgttag tcattccaat
       61 acgttgagaa attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt
      121 gtttccatca tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat
      181 tagatctttc atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg
      241 agaatcatac aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact
      301 tgaaattcct gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc
      361 ttctactact ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa
      421 ctgttgattt tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg
      481 cgttaaataa actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa
      541 atattctcca gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt
      601 tccatcttct gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc
      661 attaatcacg gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta
      721 gcttttatat tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga
      781 agctttattc gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga
      841 acttgcactt gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt
      901 tttacttttt ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt
      961 ctcagatgta caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt
     1021 cattatattt ccccctttca atttctccct cacgtactta ttctatcaga caattaattg
     1081 atctaaatgg taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag
     1141 cggtctcttc cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat
     1201 cctctacagc gataagttct cattattttt gggctattag tattattttt cttagttaga
     1261 gctatgagtc caactatgat taccccaact gtcttaatta cttttccttt atctatattc
     1321 ggatgcgcaa gcatatattg atataaccaa tatccaccgt aaattaaacc acctaataca
     1381 atcagcttag ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct
     1441 tgcagagaaa ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat
     1501 catgttttcg attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat
     1561 ctgttgccct cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc
     1621 ctcaaaaaag tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat
     1681 ctacacaccc cctgttattt tttacataat gaaaatagca tattactggg tacattgata
     1741 attcatataa catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt
     1801 ataagtaaca tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca
     1861 attgccattc attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta
     1921 tcaatacttt ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt
     1981 gttcataagc cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga
     2041 ttttcttttc tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa
     2101 gaaatacccg aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag
     2161 taaatcctaa ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg
     2221 gttctgtatc tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc
     2281 ctttgatcgc ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa
     2341 cttctgtttg tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt
     2401 ttgttcaagc acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt
     2461 catattcttt ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa
     2521 catttcgcaa tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca
     2581 aaccaatcgt ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt
     2641 aatgaaataa actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt
     2701 catcactaaa cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt
     2761 ttccatcgta acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt
     2821 ttccagtact tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag
     2881 tattcttctg gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga
     2941 gacactgatt tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct
     3001 tcttgacgat gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga
     3061 tagtctaaag gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt
     3121 aattccaccg gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga
     3181 aacatttctt ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg
     3241 gcttcatgga tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact
     3301 acacaattat taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc
     3361 gtggtgctag ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa
     3421 aaaacgcata tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat
     3481 ggttacgtcg tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat
     3541 atccccgatt atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat
     3601 cgtgttttaa aaggcatcaa aatcggttac atcgcttctg aaaaggatcg gatcatcgac
     3661 tatttagccg ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt
     3721 gattatttag atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt
     3781 gcacaacttt cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat
     3841 ggttttcggg tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta
     3901 ccgattctag atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct
     3961 tacttgtatg acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt
     4021 ggtaccttgt ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc
     4081 gatgatcttt ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa
     4141 ggaaagcgaa ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag
     4201 gacgtttttt tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg
     4261 aaaatgtttg atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg
     4321 atttttttca taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt
     4381 cgcttcatcg agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag
     4441 ccgttgaatc tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg
     4501 atgatcctta ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc
     4561 agttgtcaat tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa
     4621 cgattcataa aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa
     4681 atacgctagt gtgatttgat tgttcatcta actcctcctt ttgacatagt atactataaa
     4741 t
//
LOCUS       DAYLZO010000168         4673 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.174, whole genome shotgun sequence.
ACCESSION   DAYLZO010000168 DAYLZO010000000
VERSION     DAYLZO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.174"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..351)
                     /locus_tag="ACGVJG_002838"
     CDS             complement(<1..351)
                     /locus_tag="ACGVJG_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFJ6558734.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQ"
     gene            complement(352..1134)
                     /locus_tag="ACGVJG_002839"
     CDS             complement(352..1134)
                     /locus_tag="ACGVJG_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFJ6558735.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(1127..2044)
                     /locus_tag="ACGVJG_002840"
     CDS             complement(1127..2044)
                     /locus_tag="ACGVJG_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFJ6558736.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(2208..2462)
                     /locus_tag="ACGVJG_002841"
     CDS             complement(2208..2462)
                     /locus_tag="ACGVJG_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558737.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            3319..>4673
                     /gene="ecbA"
                     /locus_tag="ACGVJG_002842"
     CDS             3319..>4673
                     /gene="ecbA"
                     /locus_tag="ACGVJG_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family collagen-binding adhesin EcbA"
                     /protein_id="HFJ6558738.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KP"
BASE COUNT         1467 a          950 c          862 g         1394 t
ORIGIN      
        1 ttggatgccg attcgtaaca aaagttcttc gatatcaaat ggtttcgtca catagtcatc
       61 cgctcctgct gccaacaagg aaactttatc tctttgatcg ctttttgctg aaagaacgat
      121 gatcggaaca ttcgatgtct gtcggatttc tgctaacaat gcttcacctg aaagccctgg
      181 aagcatgaga tctagaagga tcaaatcgac aggatatgtg cttaacaata attttccttc
      241 cgtacctgaa taagcatcaa gaagtttgta tctctgttca agaacctcct ccagtaagga
      301 atgaacagct tcatcgtctt cgataatcaa aatagtttcc agcaggacca cctacttaat
      361 gtcagatttt tggaaaataa acattcctag agctgatgat accacgattg ttactagact
      421 gaccagaatc attttcggtg cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat
      481 gagccacgtt tccgtaaacc actccgttag aaattccaag cctttgattt ttgcgaaaac
      541 accgggaatg aaggccgttc ccatgatata aagaaaagaa agaagcaaac tgacactggt
      601 acttctagtg atgaatgtca aagccactgg aacagataat gtccctaatg tcagcagaag
      661 caatacagga aatactttta gaacataatt tccatagttt ttgattccct caacttcacc
      721 aaatccgaaa gcaagtgtgt aagcaagcat ggctagtccc gtgatcaaaa ccacgccaag
      781 tgttgtgatc aaaagcgtaa cgagaagttt cgacacataa taagtcattc gattccgtcc
      841 taaagataac gtattgcgaa tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc
      901 aaatgctgcg aaagccagaa gaatccatgc agccattttt tctaccatta aaatccctgt
      961 tgcaccgttg atgactgtat ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat
     1021 ataccattcg ttccaagaat aaaaaagcgg aagtacgacc atcagtccta gtgagaccca
     1081 cattttacgg ctcttccata cttttatttt ttcagcagtt aatagattaa acatgtggat
     1141 tgcctccagt taattgtaag aaataatctt ccagtttttg cccaactagg tgaataccat
     1201 ccacaggaac ttttgcttct accaatgctt gattgatggc tgctacttgg tcaattcctt
     1261 caaaaagctg gataacacgg ctagattgta caaaataatc cctataacca agagagtcga
     1321 gaaccgttac tgcttgttcc gtcgccgaag tgtcaatttt tataaattga cgagattctt
     1381 gtgccaattc ttctttcgat aattctttga tcaagtgtcc ttcatgcaaa ataccatagt
     1441 gagtggcgat ctgtgacaat tcatctagca agtggctaga aagcaagacg gtgatgcccc
     1501 gttcttttac caaacgttgg atgatttctc gcatttctgc catccctgct ggatccatcc
     1561 cattcgtcgg ttcatccaaa atcaaaaatt ctggattcgt gatcagtgca tttgcaatag
     1621 ctaaacgctg gcgcatacct aaagagaaat tctttgcttt ctttttccct gtattttcca
     1681 atcgcatcat tttcagcagg tcttcgattt ctctatccga cacaccgccg ttcgctgctt
     1741 ggatacgtag gttatctctt gcagttaatt ctggataaag agcaggggtt tcaatcgatt
     1801 gacccatacg tcgtcttgct gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt
     1861 catcaatcga gatcaaaccc ataatagttc tcataaaggt agacttaccc gctccatttt
     1921 ccccaatcaa tccatagatc attccacgtt tgatttcgat tgatacttta tctaatgctt
     1981 tggcagcacc gtattttttg gttataccag ttgctttcaa gaccgtttca tttccaaaat
     2041 ccatacttat ccttctttct tattgatgag tgaagtatag ttgacgggta taaagaaagc
     2101 agccagaaat gtataaagaa agtgtaaatt ttccaattga atgtcaaaaa aaacaatcgg
     2161 acaatacgtg tctttcctat tatcaggcag tttgttatac gcttgtatca gtttcctgat
     2221 tctttctttt cgaccaaagt cgtttctctg tattcctctt cactgaaaat ttttgcttct
     2281 ttcagatcaa gcttgagtaa ttttaccaga ccggttataa ggataactat agcaagactc
     2341 cctcccagaa aaattagact gtctcgcaga cgtggttgcg ttgtcttcca atcaaagagc
     2401 gcaccaaaca gtgctaatcc aagtggactg gccaccatgg taatcgttgt tatcgttgac
     2461 ataagtcgtc cttgtatatg cgttggaact tctcgctgaa gccagaccat ataaaaagca
     2521 aaagaaaaca ttaatcctta aaaacggact tatgaaaaat aatacatgcc gtttgggatt
     2581 tacaattagc taccaaataa gaggttatga caaaagactt ttcacaatcc cttcttccga
     2641 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatttat caaacatgag
     2701 aagcaatttt tgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctca
     2761 gaagcggata aataatattg tttgatgata ctttatcttt ttcccgtttg tttatagatc
     2821 gaatgatatt atagaccaag aatcctacct attaacatag ctaaaaacac aaaaaaggca
     2881 ctttccaaat tgtggaaagt gccagaaaag ttaggattaa ggtaactgcg ctcagcattg
     2941 cttcgcccag tacgattctc gtaactcgtg aactcgaacg ataatcgcaa ttaacgtttt
     3001 gagaactgtg aagctttacg ggctttctta agacctggtt ttttcttcgt atcattgaat
     3061 aagtttgaaa aaccttgatt tataaaggtt ttcccgtaaa tatattttat tatatttgat
     3121 taaatttgaa atgtttgtgt tctaaaatgt gttctaattt aaaggcagta tatttcgtat
     3181 tttagattaa atttttaggc tcattcattc tcaaatccat tctattcagt caataaaaat
     3241 tttaaaaaga ctttataaac aaagatgttc aagcgagcat cttatttttt tgtgcttaat
     3301 ttgaaaggag aacatttcat gtataaaaaa caaaaaaaga aattatgtgt attaactatt
     3361 ttgagtttgg ctgttcaagc aattgttgga gcaattgctc ctacgattac ttttgctgat
     3421 gaaattaccc atccacaaac ggtaaccgta gaactagatt tagcacatca atatgctgtt
     3481 gaaggaactt ttagtgatgg tcgtccaatg tcagaagtca ctgttccgca ttatgcagtg
     3541 tacaacggct ttaaacaaga tattttctgt attgaaccag gagtcccgat ttacaatgag
     3601 ttcactcctg gctatgagaa aaacccgtta ccagatatgt ccgataaagc caaattagtt
     3661 tccgttttat ggaaaaaagt aggtacagat gcagatactc atattgtggc acagaagatg
     3721 atctggcaag aagttaatgg gtataccctt cactcaataa aacgatcaga tggtagtgcc
     3781 gtaaatatcg cagccattga ggcaaaaata aatcaagcca ttgacgatta tcagaagaaa
     3841 ccaaccttcc ataatagtac ggctaaaaca gtattagggc aatcgactac tgtgacggat
     3901 acgaataatc tgaatttgtc agagttcgat gaggttgtgg aaaatgccgc aaacattgat
     3961 tatcgtgtga atggtaatca gttgataatc accccaaatg ccaattcaaa agaaagtggt
     4021 gtgctgacgc ttaagaaatc tgctggtacc ggaacacctg tcgcctataa aaaggctggc
     4081 caacagactt taatggccgg ggcgattgat aaacctaata cctacacaat caaaattgat
     4141 gtggaaacgg agggttcttt aaagatcaaa aaagtcgata aagaatcggg tgatgttgta
     4201 ccaggaacgg ttttccattt agactttgga aaaactttac ctgcaaaaga cgtgactact
     4261 gacaaagaag gcattgctac attggatgag attcctcatg gtacaaaagt aaccattacc
     4321 gaaaaatcag taccggcgcc ttatacgatt gataccaccc ccatgactac caccattaaa
     4381 gcaggcgaaa ccatttacgt gacttcaaaa aacgcacggg aaaaagggca aatcattctg
     4441 gataaaagcg gagttgaaac agggagtgat ctttggaatg acaattattc cttagctggt
     4501 aatacgtttg ccattcgcaa agacagtcca accggtgaaa tcgttcaaga aatgaccacg
     4561 gatgaaaacg gtcatgcgga aacaccaaaa gagattgcta atgcgttgga attgggaacc
     4621 tattatgtga ccgaaactaa agctagtcat gggttcgtga atacgttcaa acc
//
LOCUS       DAYLZO010000169         4669 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.175, whole genome shotgun sequence.
ACCESSION   DAYLZO010000169 DAYLZO010000000
VERSION     DAYLZO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.175"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1209
                     /locus_tag="ACGVJG_002843"
     CDS             <1..1209
                     /locus_tag="ACGVJG_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFJ6558739.1"
                     /translation="FKVNAKAAFAVDAQSGKILYDQDGEKTMGIASITKIIGLYIVLD
                     QVKEGKLSWGDKVSISDYAENLSITPDLSNVPLHKENTYTVKELFDSAIIQSANASMV
                     ALAEKISGSEAKFTERMKEQLKDWGIKDATIVNASGLNNSYLGENRPEGTGENDENQM
                     SAQDVAIVARHLILDFPEILDVSSTTTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVD
                     GLKTGTTELAGACFVGTITKNDQRIITVVLNADGHAENPSARFNETDRLMDYCYDNWS
                     EKELGKANASIPSLKTIDVKDGKESTVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQ
                     VTDNEIQAPMDKGTKIGTATITLADDKLGYLEDNNAPSTEIITDKTVEKANIFVIGWR
                     RVAEFFGNLF"
     gene            1328..2527
                     /locus_tag="ACGVJG_002844"
     CDS             1328..2527
                     /locus_tag="ACGVJG_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFJ6558740.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(2554..3822)
                     /locus_tag="ACGVJG_002845"
     CDS             complement(2554..3822)
                     /locus_tag="ACGVJG_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFJ6558741.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(3877..3943)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(3985..4631)
                     /locus_tag="ACGVJG_002846"
                     /pseudo
     CDS             complement(3985..4631)
                     /locus_tag="ACGVJG_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
BASE COUNT         1426 a          973 c          871 g         1399 t
ORIGIN      
        1 ttcaaagtaa atgcaaaagc agcttttgct gtagatgcgc aaagcggtaa gattctttat
       61 gatcaagatg gagaaaaaac catggggatc gcttcaatca caaaaatcat cggtctttac
      121 atcgtccttg atcaagtaaa agaaggaaaa ctgtcatggg gtgataaagt ttcgatttca
      181 gattatgcag aaaatttgag tatcacccca gatttatcga atgttccttt gcataaagaa
      241 aacacctaca ctgtcaaaga attattcgac tctgcaatca tccagtcagc caatgcttca
      301 atggtggcct tggcagaaaa aatctctggc agcgaagcta aattcactga acgaatgaaa
      361 gaacaattga aagattgggg aatcaaagat gcaacaatcg ttaatgcatc aggattgaac
      421 aactcttatc ttggcgaaaa ccgaccagaa ggcactggag aaaacgacga aaatcaaatg
      481 tctgctcaag atgtggccat cgttgcccgc catttgatct tggatttccc agaaatactg
      541 gatgtatcaa gtactacgac ccaaatgttt ggcgaaaata cgcaatcacc agtggaaatg
      601 gtcaactgga actggatgct cccaggattc gttaattaca aggaaggcgt ggacggatta
      661 aaaactggta cgactgagct tgcaggtgct tgtttcgttg gtacgatcac taaaaacgat
      721 caacggatca tcactgtcgt attgaatgcg gacggccatg cggaaaaccc gagtgcccgc
      781 ttcaatgaaa cagaccgttt gatggactac tgctatgaca attggtcaga aaaagagctc
      841 ggaaaagcta acgcaagtat tcctagtcta aaaaccattg atgtaaaaga cggaaaagaa
      901 tcaacagttc ctgttgtgct taaaagccct gtaaaagtat gggtacgaaa cgatatggat
      961 actggcaaat taacgatcac gccaacactt gacaagaagc aagtcactga caatgagatc
     1021 caagcaccta tggataaagg tacaaaaatc ggtaccgcta cgatcacctt ggcagacgat
     1081 aaattaggct atttagaaga caacaatgca ccatcaacag agatcattac ggataaaaca
     1141 gtggaaaaag cgaatatatt cgtgattggc tggcgacgcg tcgcagaatt tttcggcaac
     1201 ttgttttaat tgaacaataa gaaaaacatc tttccatcgg aaagatgttt ttttgctttt
     1261 tctagtttaa ataacagctt ttttcatcta ttttcgttat actgtataag aaaaaggagt
     1321 tgaatttatg aatctaacca gagatctgat ctttttagtt ctctatctta ttggggtatt
     1381 ctctatcgtt tattggcgaa aacagaagaa ggatccttcc ttgttctttt acggcagtac
     1441 gtggacattt acggtaatct taacttattt tgtcgtgttg gaaatttttt attcgacctt
     1501 ttattttttg attccattgt tgttctttag tatctttgct ttttcttatt tcacagaaaa
     1561 acgaaggcta ctgaatggtc ttttattcaa tgttttactc atcagtttcg gcatctactt
     1621 gtttgtcttg ctgtatgaaa cgcaaaatat ttttcttggc ggactgatcg ccttgatcac
     1681 gattccattg ctacttgtcc ttttatttgg tatttatgga ttgattgttt ttcttttttg
     1741 gaacggggtc acagttttac gtagagaatc ccattcatta gctaatcttt taacgttgat
     1801 actagccatt tttcttacac tgtttcttgt ttttgacttt ttcttgttaa aatatttgcc
     1861 gcaatggata aatgctttgt ttttctgcgt tcccctgatc ttgatctatc tatttatcgt
     1921 attctacaac tttttgactg tctcattctt gtatcagttc aaccggcctc gatacaatca
     1981 agacttcatc gttgtgttag gcgctggatt gatcaatgga gaaacagttt ctcctcttct
     2041 agccaagcga atcaataagg caattgcttt ttatcgggct caaagccgtg ctacattaaa
     2101 tccgcccatc ttattgatgt ctggcgggca aggagcagac gaaaaagtac cagaggccat
     2161 tgcgatgaaa caatatgcca tggaacaagg aatccctgaa agagacattc ttgtcgaaac
     2221 caattcgaca accactttgg aaaacatgct ctattcaaag gaaatcatgg atcagcaaat
     2281 gaaagggcca taccgcgcga tctttagttc caacaattac cacattttta gagccggtct
     2341 ttatgcgcgg caagccaaat tgaaagcaaa tggtattggt gcaaaaacag ctttctacta
     2401 cttgcctaat gcgtttttaa gagaatacct cgccatcttg atgatgaaca aaaaaagaca
     2461 tatgatcatt tgcggattgc ttttcgcagg ctccgcattc ttatcgctga ttactttact
     2521 tttctaaaaa aacaaggtgt gacaaaacat atgtcacacc ttgttttttt ctttcattct
     2581 cttaactatt ggcaaaaaga gtatcccttt tttgaccgct acttgctgag cagaataaat
     2641 accgctgttg cctgaacaca taaagctgat caagcagatc ataaagaagt atactgccgc
     2701 ttgtgaaccg aacaattcga tccccatcac aaagcaggca atcggtgtat ttgtcgcacc
     2761 agaaaatact ccgataaagc ccaatcctgc aagaaaagga acagaaacat ggagcaaagg
     2821 cgctagtgct gcaccaagcg ttgcaccaat ttcaaacaat ggtgtcactt ctcccccttg
     2881 atatcccgcg cctaaagaca aaactgtgaa gaataacttc ccaataaaat catagacatg
     2941 tgcctgtcca ttaaatgatt cttgaagtaa cggaagactc aatccgagat aacgttgtgt
     3001 ttgcaaaatc aatgctgctg ccacaactac agctccacct atcgtatttc gacaaacagg
     3061 atgaggaatc cattgtgcat atcgcgcttt taaaaagacg atcgaacggc tgaataccca
     3121 accaatcaaa ccaaaagcaa tcccagcaac aaaaactttg aagaacagca acatactcca
     3181 ttcaggaatc acacccattt ggtaatgtag atgagaaaca ccatagcttt ctgtcatgaa
     3241 atttgcaaac agtgcagcaa agaagctagg gaagatcgct tctgttcgga ctttcccgat
     3301 tgccaacact tctaatccaa ataatgttcc agctaaaggt gtgccaaata cagaactaaa
     3361 acccgcactg attccactga taatcaagat ttcccgttct gatttatcca atcggaacaa
     3421 acgtgccgta ttatctgcta acgctccccc catttggact gctgttcctt cacggccaac
     3481 agatccccca aataaatggg tagttatggt tccaaacagt gtaagtggaa taagccgcaa
     3541 cgggattttt tcttctccac catttccttg atcgatcact aagttgtttc cacgactaga
     3601 aagtccgccg tgatacgcat agagataagc aaaaagcgca ccactaactg gtagtagaca
     3661 aaggagccaa ggatgtgata gtcggatatc tgtcacaact gtcaggctat ttaagaaaaa
     3721 agcagacaag ctccccatga atataccgag aatcgctgtg atcaccagcc acttcagcat
     3781 atacactaaa atgcgtactg gactcggtaa ttgtttttcc ataaaaaatt cctcctcaat
     3841 aaaaaaacgc ctactaaaga aaggggctgt ccccagactt tagtaggcgt cattagctat
     3901 ctttcgttag catttaaagg cgaacaccat cacctattca cttccattaa aagatagcat
     3961 atttttctgt aaaaaacaat aaaattatcg ttctttcttt acacgttcag caaattcata
     4021 gaaaggagcc aatttcgtta actgttgatt acattggtca ctatcatgac aaatataatt
     4081 cccctttttc gtatacgtgc catcagaacc tgctttcgtt gtagaaagaa aaagcgaaac
     4141 attcgatgtt ttctgacaga ttgcacagat tcccttattt atcgttggtg ataaagtacc
     4201 gctaatccct tttaattttt catcttcgta atagaccatg aattttttct gcgtacctgg
     4261 atcattccag ccgatgtacg tatgttcttg caagtcgatt tcattccaag ctggttgttt
     4321 gagttttttc acttttcgga ataatttctc gatttgtggt ttgctaggct gcataaacgg
     4381 ctgaacgatt tgcttcagct cctccaatgc tctttctgct tgcgcttttg tcagttttac
     4441 gtctaaaact gtcgttaaaa atgtatcgat tctggcagtt cgtgatctaa tatgtctcga
     4501 atcttttcag ctgctagtgc ctgaactgtt tgtaccgttt ttggatcatt gacagatgag
     4561 tacgcattta ataactgttc tacttctctt attataaaaa aatattggta tggttttatt
     4621 gtttgttcca tgattttaaa gtcctttctt ctaaccaaag tgcatactt
//
LOCUS       DAYLZO010000170         4665 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.176, whole genome shotgun sequence.
ACCESSION   DAYLZO010000170 DAYLZO010000000
VERSION     DAYLZO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.176"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(192..662)
                     /locus_tag="ACGVJG_002847"
     CDS             complement(192..662)
                     /locus_tag="ACGVJG_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFJ6558742.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="ACGVJG_002848"
     CDS             992..2632
                     /locus_tag="ACGVJG_002848"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFJ6558743.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="ACGVJG_002849"
     CDS             2786..4108
                     /locus_tag="ACGVJG_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFJ6558744.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4607)
                     /locus_tag="ACGVJG_002850"
     CDS             complement(4176..4607)
                     /locus_tag="ACGVJG_002850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6558745.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
BASE COUNT         1340 a          909 c          873 g         1543 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc tacgccagtc
     4321 atcaaagcct ttccgatgcc tcttttttca gcatatttag acacagcaag tgccataata
     4381 ttgaacattg gatcgctaaa aatcgattca taggactccg cttgtacata gccagctgct
     4441 tttttagaag taggatcttc ataaaccaag aaaaaatttt tctctggctc cttcagcagt
     4501 ttcttcattt gtctcaccgt aacggttaat ggtatatcat accctaattg atccgcattg
     4561 atttcacgta gtgcttctgc atcttctaaa cgcatttccc gtaacatcca aatcaccagc
     4621 caatttctga ttttttaaaa tgaatttttt tatcatagta tactc
//
LOCUS       DAYLZO010000171         4615 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.177, whole genome shotgun sequence.
ACCESSION   DAYLZO010000171 DAYLZO010000000
VERSION     DAYLZO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.177"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..285)
                     /locus_tag="ACGVJG_002851"
     CDS             complement(<1..285)
                     /locus_tag="ACGVJG_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HFJ6558746.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPY"
     gene            463..1563
                     /locus_tag="ACGVJG_002852"
     CDS             463..1563
                     /locus_tag="ACGVJG_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFJ6558747.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            1553..2377
                     /locus_tag="ACGVJG_002853"
     CDS             1553..2377
                     /locus_tag="ACGVJG_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFJ6558748.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            2374..3189
                     /locus_tag="ACGVJG_002854"
     CDS             2374..3189
                     /locus_tag="ACGVJG_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558749.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            3186..4016
                     /locus_tag="ACGVJG_002855"
     CDS             3186..4016
                     /locus_tag="ACGVJG_002855"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558750.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            4023..>4615
                     /locus_tag="ACGVJG_002856"
     CDS             4023..>4615
                     /locus_tag="ACGVJG_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558751.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMR"
BASE COUNT         1257 a          876 c         1156 g         1326 t
ORIGIN      
        1 ataaggatgc tgagcgaggt aatccacgat ccgctcttct tgtcctggaa caacgccaat
       61 ctcgatacct cgcagaaatt tggtggaata ggtttctttt aaagagtcca gtttgtttat
      121 ataggctgga taatccggaa tgtcatcttt tccggtagat ggattgttta aatcaaagtg
      181 atccgtagaa acgaaaaact ccggctgata ggctaaatag ttttcaaatt tttcctctga
      241 atcaaaggaa aaatacgtgt ggagatgctg atcataatac tgcataggga gcctcctttt
      301 ttctactatc ataccgcaaa agctttagaa ggttaacggg aattcgtcga aatttacaaa
      361 ccatatacaa cctttacatt gttcatgaaa ccttttcaag aaactgattc tctcattaca
      421 aagctttgtt agaatggaat tatcgaaagg ggagaagaat gaatgaaaat gaatgaaaaa
      481 attcgtaaca tgactatcgg tttattttgt gcagcaacag taagtatcct gctgacaggc
      541 tgcggctcag gtgaagcaac gaagcaatcg gctgcggcag acgactcttt agatacgatt
      601 atagaaaaag ccaaggaaga aggagagatt gccagcttgg gaatgccgga tacttgggca
      661 aattgggtag gcacttggaa cgaccttgaa aaagaatacg gcttaagtca cgtggatacg
      721 gatatgtcca gtgcagaaga attggctaag tttgaatcag aaggaaaaaa tggaaccgcc
      781 gatattggtg atgtcggtat cagcttcggt ccgttggctg aaagcaaaga gttaacctta
      841 ccttataaga ccaactattg ggaggaaatt ccggattggg caaaggatga tgatggggat
      901 tggttgttaa gctatacggg aaccatttcc tttatcaccg ataaagagaa tgtgaagcaa
      961 gcaccgacta gctggaagga gctgcaagag ggtgactatc aagttagcgt gggggatgtg
     1021 acgattgcca accaagcaca atttgctgta ttggctgcgg cgattgccaa cggaggtgac
     1081 gaaaaggata ttcagccagg tctgaattac tacaaaaaaa tggcagaaaa aggccgtttg
     1141 tccaccgttg atgccagcgt agcaaatctt gaaaaaggtg aaatcgatgt ggcagttgtt
     1201 tgggacttca atggactaaa ttatcgtgac caaattgact ccgagcgttt tgaggtaact
     1261 gtgccaacag acggctccat cattagcggg tatacaacct taatcaataa aaatgcccct
     1321 catcccaatg cggcaaagct agcacgggaa tatattttat ctgatgaagg gcaaatcaat
     1381 ttggcaaagg gctatgcgcg acctattcgc aaggtagagc tgcctaaaga agtagctgaa
     1441 aaattactgc cggaggatgc ttatgaagcg gcggtgccag ttgaagacaa tgaagcttgg
     1501 aacaagacct ctgtagagct tccagagctt tggcaggcgg aggtactgac ctatgcaaag
     1561 tagacttgac aaactgattg tagttatact agacggctgt cgctacgaca cagccgtcgc
     1621 ccagcttggg ttcatgaatc atttagttga gcaccagcaa gcaagcttat tgaaggtaaa
     1681 aagtgagctg cccagcaatt cacgtccgtt atacgaagta ctgatgaccg gcgttccagc
     1741 ctatgaaaat ggcatctata gtaattacca tgctcagcga tccaaagagt ggtcgctgtt
     1801 tgaattagtt aaggaagctg gcggaaaaac aggagccgcc gcttaccact ggtacagcga
     1861 gttgtacaat caagccccct ataatcgttt ggttgatcgc atccagcatc aggaggagcg
     1921 actgattcag catggcatct tttatagtga ggacacttac cctgattcgc atttatttgc
     1981 ggatgcccat tatttagtga atcagtatca gccagacttt ttggtcgtac attcgatgaa
     2041 tattgatgat gtcggtcaca aatttaccgc cgattctcat gaatatgcag cagcggttaa
     2101 tcaggcggac agattattgg ctatttacct tcctgaatgg ctgcaaaaag gttatcaagt
     2161 cgtggtgact gcggatcacg ggatggatgc gcacggactg catggcggca gcttggctgc
     2221 acatcgtgaa gttccttttt atttgatttc aaataaacgc caaaagctga aggaacaaac
     2281 tgtaccacag ctgctggcgg ctccattgtt ttgctggtta ttaggaattg caccgtcaga
     2341 gaaaatgcag gacattcaag aattgatgag gggatgaaag agatgaaaaa acgcttggtt
     2401 ctggttcctg cctcctttat cttctgtgca tttttactgc tgccgctgct ttttatggtt
     2461 ctgtgcagct tgcacagtga tttaactaac cagtggacat tggccaatta cagtgatatt
     2521 ttcagcaaca aatattacac acaggccttt gttaacagcg tactgattgc tgtcagctcc
     2581 agtgtgattg ggttaatcgg gacgtttatt ttatgtttat gcttaatgaa tctatcgact
     2641 aaattgcagg agaaaatcac ctttgtttcc aatttggcag ctaattttgc tgggattccg
     2701 ctggcattct cttttattat tttgttgggg aatgtaggtg tgctgaaaat gattttgcca
     2761 tttctagcgg acttcaattt gtattcttgg tggggattga ccatcacgta tacctatttc
     2821 caaattccgt taggggtctt gtttttgtat cccagcatga aggaagtgaa aaaggaatgg
     2881 ctggagtccg cacaattgct gggcgcttca tcagcgtatg cttggttcaa ggtggctttg
     2941 ccttttttgc gaccatcgat tgccagcacc tttgtcatct tatttgctaa tggcatgggg
     3001 acctatgaaa cagcgtatgc cctgaccagc agtaatgtaa atcttttaac gattcgaatt
     3061 gcggctttag tgtcgggaga tgtgttcgcc aagcctaatt taggcagtgc gttggcagtc
     3121 gctttaggtg tcctgctggt aacggcaatg ttgattattc aaaagaaagg caaggtggtg
     3181 cagacgtgaa aaagttaata aaaccgattt tgttctgctt tgtcctgtat tacttgctgc
     3241 cgattattgg aacgattttg tatgccagct ccactaaatg gtcaaaatcg cttttaccta
     3301 gtgattttac cttgcaatgg ttccaacagc tgctgacgga cagggaattt attgctgcgg
     3361 tgggtcgctc acttttatta gcaggtgtgg tcttggtgac gattctgttg ttgatgattc
     3421 ccaccattat ttggattcat ttgtatttcc ctcgtttgaa tcgttggctg gaaaagctgc
     3481 tcctgctgcc gtatgcttta ccaggagtga ttttggtaac ggctttgctt cgaacgtatg
     3541 ctgaaactgg tatcccaatg tttgtagtgt tggtaggtgc attgtttatt acggcgttgc
     3601 cgattgtcta tcttagtctt aacaaccaga tgcgcttaat taatctgaag gaattagtgg
     3661 atgcagcaga aaccttgggc gccccgatgt ctacgattat tatccaggtg ctgtttccaa
     3721 acatccgtat cggtgtgacc ctagtttctc tgatgatttt ttcttcggtt tttggggagt
     3781 acatgctgac gaacctgttg attggcggtc gatttcagac ggtacgcatc tatatgctgc
     3841 ggcggatgaa cgaaaacggc catttggcta gcgctgtgat ggttttgtat ttgctgttta
     3901 tggtgggaat tgctttaact acctttatgc tgacttatcg acaaaagcaa cagctgtccc
     3961 ttaagcagac gagggaagag aagcagtcga aaaagaagcc cttaaaggag ttgtgacgga
     4021 aaatgttttt agaaatacat gatctcaatc tgttttttga tgaaaaacag attttagcta
     4081 ttgatgaaat tcagattgaa aagggtgagc tggttacttt actaggtccc agcgggtgcg
     4141 gaaagtctac gttgctgcgc tgtatcacgg gattggaaca accaaagagc ggtgagattg
     4201 tgctggacaa tgagaatatt gccgataagc ctacgaagga tcgcaatatc ggctttgttt
     4261 ttcaacagta tgcattgttt ccaactatga ctgtttttga aaatgtagcc tttggtttga
     4321 aggtaaagaa gctgagtcct gaggtgattc aggaaaaggt ttttgagatg ctatcattgg
     4381 tggatatgac ggagcaggca gataaaaatg tccagtttct ttctggcggt caacgacaac
     4441 gggtggcact ggctcgctca ttggtcactg aaccgaaagt cctgctgctg gatgagccat
     4501 taagtgcttt ggatgcccga attagaaaac agctgcagcg ggatttgcgc gctatccagc
     4561 aatcgctagg gatgacgatg atttttgtga ctcatgacca agaagaggcg atgcg
//
LOCUS       DAYLZO010000172         4589 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.178, whole genome shotgun sequence.
ACCESSION   DAYLZO010000172 DAYLZO010000000
VERSION     DAYLZO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.178"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..505)
                     /locus_tag="ACGVJG_002857"
     CDS             complement(<1..505)
                     /locus_tag="ACGVJG_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558752.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILIS"
     gene            471..2108
                     /locus_tag="ACGVJG_002858"
     CDS             471..2108
                     /locus_tag="ACGVJG_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558753.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="ACGVJG_002859"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="ACGVJG_002859"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFJ6558754.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="ACGVJG_002860"
     CDS             complement(3184..3819)
                     /locus_tag="ACGVJG_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFJ6558755.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..>4589)
                     /gene="sstT"
                     /locus_tag="ACGVJG_002861"
     CDS             complement(3966..>4589)
                     /gene="sstT"
                     /locus_tag="ACGVJG_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFJ6558756.1"
                     /translation="QLLLVLIGTMLFVALVVYPFLTFLFIKENPYPLIFFCLKESALP
                     AFFTRSSAANIPINMMLAERLKLTKESYSISIPLGATINMGGAAITISLMTLTAVHSL
                     GIEAPFVLKVLLCILSALAACGASGVAGGSLLLIPLACSLFGISNDVAMQIVGIGFII
                     GVIQDSMETALNSSSDLLFTAIGELGARKRSGEKIKLKDCLDGLSER"
BASE COUNT         1522 a          850 c          673 g         1544 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctg
//
LOCUS       DAYLZO010000173         4588 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.179, whole genome shotgun sequence.
ACCESSION   DAYLZO010000173 DAYLZO010000000
VERSION     DAYLZO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.179"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(320..694)
                     /locus_tag="ACGVJG_002862"
     CDS             complement(320..694)
                     /locus_tag="ACGVJG_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558757.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(811..1293)
                     /locus_tag="ACGVJG_002863"
     CDS             complement(811..1293)
                     /locus_tag="ACGVJG_002863"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFJ6558758.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            complement(1345..1923)
                     /locus_tag="ACGVJG_002864"
     CDS             complement(1345..1923)
                     /locus_tag="ACGVJG_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6558759.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            complement(1952..2953)
                     /gene="ruvB"
                     /locus_tag="ACGVJG_002865"
     CDS             complement(1952..2953)
                     /gene="ruvB"
                     /locus_tag="ACGVJG_002865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFJ6558760.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            complement(2966..3568)
                     /gene="ruvA"
                     /locus_tag="ACGVJG_002866"
     CDS             complement(2966..3568)
                     /gene="ruvA"
                     /locus_tag="ACGVJG_002866"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFJ6558761.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            complement(3738..4184)
                     /gene="msrB"
                     /locus_tag="ACGVJG_002867"
     CDS             complement(3738..4184)
                     /gene="msrB"
                     /locus_tag="ACGVJG_002867"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFJ6558762.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            complement(4261..>4588)
                     /locus_tag="ACGVJG_002868"
     CDS             complement(4261..>4588)
                     /locus_tag="ACGVJG_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFJ6558763.1"
                     /translation="INGEVVILDTDYLLKEIVPILTPAQAQGSIYEYFENELSLTIAY
                     AQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSESVHRLDKFRFVEF
                     ARRRKV"
BASE COUNT         1250 a         1003 c          780 g         1555 t
ORIGIN      
        1 aacttaacaa ctttcgaaaa ctttcgtcac aaaatattca caactaatga ctattatata
       61 agcataccaa caaacaaccc taacaacact ttttcatttt taactcctta tcctgctagc
      121 tccccggcta gcaggacctt tttttatcaa aaaaacttgt accatccaat cgattcaact
      181 aatcaaccga atgctgtcat gctatgatac tcatcctaaa acgttccgtt ttactgatat
      241 cagcttaaag cattcggtta tttttgatta gtaatgattg atggtacaag ctcatacgtg
      301 cttttctatc tggtataaat tattttgata accgttcctg ttcccctgct ggtgaaagga
      361 ttgttatttt ttcaatttgt tctacaggaa ccatccggat ctgctgctct tcgttttgta
      421 ggcggatcat cactcggtct ttttcgaccg tttttgtcgc cacccagcct gagatcgttt
      481 catacttgct actcaaacga tttgctctta cttgtaagat aactagacta tgttcagtag
      541 cagctttttg cagctcaaga ataaactttc cttgcaatcg tacattctta gcatgttctt
      601 cagtgtcatt tacaaaagaa tcaaaaagtt gatacgaagt atgagtaact aacttcgtaa
      661 gcttcttcat tggcgatact gtctcttcgt tcatcgaatg cactcctcct ttagctaaga
      721 aagtgttact taattaattt tattttacct aacattttac aaaaaaacaa ggaaatgctc
      781 taacatcgcc ttaaaaagaa agcgttaact ttacaaattg tttgttattt tattttcatc
      841 aatccaaata ttccaccaat gattcaggcc ttctgtgatc aagtgatatg tctcatcaaa
      901 gttattggta tagtatgggt cgggaacatt ttctttttct tttccaggaa tgctacttaa
      961 aaacaaatgg attttcccct gcgctgagcg tggtgctctt cgtttcagtt cttctacatt
     1021 ttcttgatcc atgccaatga tccaatcaaa agtttcaaaa tcgtggggtg tgatttgccg
     1081 agcaaccata ttcgtcatat ctactttttc ttggtttaat atttttttcg ttccagggtg
     1141 cggtgtcttt ccaacttcat acgtgcttgt agcagcagaa tccacaaaaa attctgcttc
     1201 ccttccttct aatgctattc gttttctcag caagccttct gccatcggtg aacggcaaat
     1261 atttcccaaa caaacaaata aaatttttgt catttcccca tctcctttta gcgattattg
     1321 cttttccaac aaaaaatttt tctattactg ttcttccaac tcttcttttg cataaagacg
     1381 ataagaatat aattccgtat ccttggctaa acggaaaagc tgcatagcga tttccatcgg
     1441 tgtttcttca tattcattcg tgatccaatt gactaataca ccactgcaac cgtaagaaaa
     1501 aaaacgagca taaaatagtt tgtctcttgc agaaagttgt ctttcttcat ccatttgttc
     1561 aaaaatcttg ataaatgatt tttggatcag ttgagaaaac tctttttgta aaatttctgg
     1621 gtcagaaagc acagtatttg cataaaattc tttcttctca gcaattgctt tcagcatttt
     1681 caatgcttgc tcttcccaat tatcaagtga aagacattgt ggatctaaat aatgcaatgc
     1741 atcctcacaa tagacccaac gaagcaaatc gtatttatct gtaaaatgat aataaaatgt
     1801 ttgtcgattg atttttgctt cgtttgcgat tttttggacg ctgatttttc gaaaagattg
     1861 cgtttggcac aaatcaatca atacgttttt gatcaattgt ttcgtacgtg ttgtatcact
     1921 cattcatttt cctccaatct aaaagcaaaa atcatatatg atagtcgtaa ccaaaatgtt
     1981 cataggcata agcagtagca attcgccctc taggtgtacg ctttaaaaat cctttttgga
     2041 tcagatacgg ttcatacata tcttctaccg tctcacgctc ttctcctatg tttactgaaa
     2101 gcgtacttaa ccctactggt ccgccgccat ataactcgat cattgttcgc aacaatttct
     2161 ggtcaacata atccaatcca gcctgatcga cctgtagcaa agtaagtgcc tgatccgcta
     2221 taggacgatc gattttcccg tctccttgga cttgcgcaaa atcacgaacc cgtttgagca
     2281 agcgattagc gattctaggc gttcctcgtg atcggcgagc aatttccaat gccccttctt
     2341 caatgatctc tgtttgaaaa atatccgaag aacgtaaaac gatctcctgc agatcctgtt
     2401 cgtcataata ttccatatga gaaatgatgc caaatcgatc tcgtaaaggt gcagaaagca
     2461 tacctgcccg tgtcgtcgcg ccaatcaaag taaagggagg aagagggaaa tgaaccggat
     2521 gtgcggttgg cccttgtcct accatgatat ctacatagaa atcttccata gctgaataaa
     2581 gcatctcttc taccactcta ggtaatcgat ggatctcatc gataaacagg acatcccctg
     2641 cttccagttc attcaagatt gctactaagt cacccggacg ttcgatagca gggccactcg
     2701 tcgtacgaat atttacggtc atctcattag caatgaccat ggccatcgtc gtttttccta
     2761 atccaggagg cccgtacaac agaacatgat ctaaaggttc ttcccgattt ctggcagctt
     2821 cgatgtaaat ctgaagttct tgtttgactt ttctctgtcc gatatattga gaaagcagct
     2881 gtggtcgaag agatttttcc aaactcagtt cattttcgcc agattctggt gataacaagt
     2941 attcatctgt catttggtct cctccttatt ttttcatcat tagcttgagt gcttggcgta
     3001 gatactcatc cgttgtctga ttatctactt cttttaattg tttttctaca cgtttgattt
     3061 ctcgatcact gtaaccaaga gccgagagag cttccattgc ttcagataga gctgtagcgt
     3121 cttttgtgtt tgcttcatca aatagactaa aaggtgtatc gatactcagc tcaccgaatt
     3181 ttccttttag atccaagatc atttgttgtg ctgtcttttt ccctactcct ggaaatttcg
     3241 tcaaataagt gacatcactg gattctactg cttgaatcaa accggagtgg tcttcgcttg
     3301 ccatgattgc aagggcactt ttcggaccga ttcctgaaac acttactaat cgcaaaaaca
     3361 gttgcttttc ttctagcgaa tcaaatccat acagaagatg cgcatcttca cgtattacct
     3421 gatggacata aatttttatt ttttgattta gtttgctact gtagcggtaa ggatttccta
     3481 gtgcgatctg gtagccgata ccattggttt ctactactat gtaatagggg ttgataaagg
     3541 taaccacacc tgtcagatat tcatacatat tctagtcctc tttcttttgc acacatacgt
     3601 tccctttatt gtagcacatt tcaccttgtg aaaaaaggag tctagggagc gtttctttca
     3661 aatagcaatc gcttgaataa aaaaagagcg gacgatcgag taaatccccg tattgtaccg
     3721 ctctttgtgt atttccatta ttctacaagt gatttgaatt ccccataacc taattcatcc
     3781 atcttttcat atggaatgaa tttcagtgag gcagaattga tgcagtaacg tagaccacct
     3841 tgatctactg gtccatcggt aaacacatgt ccgagatgag aatctgcttc tttgcttcga
     3901 acttctattc gatgcatgcc atgagaaaaa tcggcttttt cgcgcacttg tcttttcgtt
     3961 attggtttgc tgaatgatgg ccagccacaa cctgcatcat atttttctgc agagctgaat
     4021 aacggttcac cgctgacgat atctacatag atcccctttt cgtaaaaatc atcatatttc
     4081 ccgctaaatg gtcgttctgt tgcattttct tgtgtgactg cataagccat gggtgataac
     4141 ttttctttca gttcttcttt ttctggtctt attggtttat ccattaaaaa tgcccccttt
     4201 gtgatcctat catagcgcca aagtggacag atgccaacaa tttgctttct tttgaactat
     4261 ttacactttt cggcgtcggg caaactcaac gaaacggaat ttatctaatc ggtgaaccga
     4321 ctcagaatac tcgaaacagc gggtatcttc cagatgaacg aggcttctga ctactactgc
     4381 ataagtgtca tcgtgaagat ccatcgctgt tttcatgatt tccgttactt cttctactgt
     4441 gatctccttt tgcgcgtaag caatcgtcaa agaaagctca ttttcaaaat attcatagat
     4501 cgatccttgt gcttgtgctg gagttaaaat aggaacgatt tcctttaata gataatcggt
     4561 gtctaatata actacttcgc cattgatt
//
LOCUS       DAYLZO010000174         4486 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.180, whole genome shotgun sequence.
ACCESSION   DAYLZO010000174 DAYLZO010000000
VERSION     DAYLZO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.180"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..675
                     /locus_tag="ACGVJG_002869"
     CDS             <1..675
                     /locus_tag="ACGVJG_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFJ6558764.1"
                     /translation="KLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIRYSHADPSY
                     IGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYDQEIIGAVCVGLTNNTID
                     NQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSEKTIIENEV
                     DEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGKKSVIIYMTFCLKRRLSIKRPF
                     GLNPCI"
     gene            666..1451
                     /locus_tag="ACGVJG_002870"
     CDS             666..1451
                     /locus_tag="ACGVJG_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6558765.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            1420..2136
                     /locus_tag="ACGVJG_002871"
     CDS             1420..2136
                     /locus_tag="ACGVJG_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFJ6558766.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2238..3257)
                     /locus_tag="ACGVJG_002872"
     CDS             complement(2238..3257)
                     /locus_tag="ACGVJG_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFJ6558767.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYYIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(3276..4277)
                     /locus_tag="ACGVJG_002873"
     CDS             complement(3276..4277)
                     /locus_tag="ACGVJG_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFJ6558768.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         1489 a          870 c          803 g         1324 t
ORIGIN      
        1 aagctgcaga ctggtgaccc gacacatgcg attcaaggaa taacaaatga aatcatgatt
       61 gcatcgaatt tagattttat cgttgtcatg gaccctgaag gcattcgata ttctcacgca
      121 gacccaagtt acatcggtct tcctttctca agtcatgaag atgtgaaaaa agtgattgga
      181 ggacaagaat ttttctctga aaaagaagga atgttagggt tcggcttccg ctatttcgtc
      241 cctatttatt atgatcaaga gattattggt gcagtttgtg ttggcttaac aaacaacacg
      301 attgacaatc aagtcaaaga tggacaaaaa atgattgttt tttcattgat tattggactt
      361 agcgtaggca tcataggcgc cgtttttctt gcccaaaaaa tgaaaaaagt actgcttgga
      421 cttgaaccac aagaaatagc agaacagtta agtgaaaaaa cgattatcga aaatgaagta
      481 gatgaaggaa tcttagccat ctcttctgat aaaactatcc tcttgatcaa tacacctgca
      541 aaaaacattt tctaccaaac agagcagcta ttggatttcg aagtaggaaa aaaatcagtg
      601 ataatctata tgacgttttg tttaaagaga cgattgagca tcaaacgacc gttcggactc
      661 aatccatgta tttaaatggg atagaaacta tttctagcgt tacacctctt tttagcaatg
      721 accgtttttt aggagcagtc gcaactttac gaaatcgatc agaagtaatg aaattaatgg
      781 aggagcttgg ggggacgaaa caatatataa actccatgag agctcaaacc catgaattca
      841 tgaataaact tcatgtcatt tctggtttga ttgatttgaa gaagtatgaa gaagtcaccc
      901 attttatcca gcagctaaat caaaattata aaaaagagat tggccaagtc tcaagtctga
      961 tcaaagtacc tgcaattgcc ggttttttat taggcaaaat aaacgaagcc aaagaacaat
     1021 ctgttgaaat gctgttagaa agcgattctt ttatccctaa aatggagatg gatgactcca
     1081 tccattcact tttgcaagtg ttagggaact tacttgataa tgcaaaagaa gccgtgctac
     1141 aaaaacatga aagaaacgga caggtccgga ttcatttaaa ctatgacaca gaagctgaag
     1201 tattcatcgt gaccgtctgc gatgacggtg tcgggatatc agaagaaatc tcagaaaaaa
     1261 tatttacgct aggtttttcg acgaagggaa tcaatcgtgg gtttggattg aatctcatcc
     1321 attcaatcgt caaacgccac ggtggatttt tagaattttc caaacaaaca aatggcggta
     1381 caatcgtcta tctagaatta ccaaaaaagg aagatcaaaa tgatgacaaa tgtcctgata
     1441 atcgaagatg atccaatggt tgcagaattg aatcgtactt ttgtggaaag catccctaat
     1501 atgaacgtaa tcgaaagttt cagaaatgta gaagaaggaa aaaaatattt cagacaaaaa
     1561 aacgcagatc tgctgctgct tgatgtatac atgcctggcg aaagtggtat ccatttttta
     1621 tcttggctta gacagcaagg aaatgatatc ccagtgattt tgatcactgc tgcagatgat
     1681 atcgaaacca taaagaatgt gatccgctac ggtgccttag attatttgat caaaccgttc
     1741 actttgcaac gttttcagct ggcgatcgaa aagtatcttg ctttctctca gacaatccaa
     1801 acgactgaaa aagccgatca gaaattgatc gatcaagtat tcaaccacac cactcccatt
     1861 cacgaaatac catcattcat cgatttgcct aaaggtctgt caaaactgac tttaaaaaaa
     1921 ataagtgcat ttattctaaa agaagatact attttttcaa caaaagaact ggctcaaaaa
     1981 agctcactgt cacgtatatc cgtcaagaaa tatttgaatt ttttagtaga acaagatata
     2041 ttgaacgaaa gaatcaaata tttggaaatc ggccggccgc taacctgtta tgaattgaca
     2101 aagaatgggc ggaaaaagct cacagattac tgttgagtca tcgaattatc ccatacaaaa
     2161 tagtccaagt atacagaagt aacaactgtt tacttggact atttttaaac attttattga
     2221 cgaatatcca tgttttctta tcttaattga cgggctttag gaagagatac ctcatttttc
     2281 gtttttccag tttctattat ttcacgaaag ttctcgaaac ttgtcgaaat catatttgat
     2341 aatgcctcat ctgtatttga acctacatgc ggggttacaa gaactctagg gtacatttct
     2401 actaattgct ggatagcagg gtctggaatc gtctcccatg gcttgaagga tcggaaaaaa
     2461 acctctgttt catttgcaaa aacatcggtt gcaaaaccag ctaaatgatt gcttttcaaa
     2521 gctcttaaaa tagcttcatt atcttgaagc tctccacggg ctgtattaat caagatgcta
     2581 ccttttttca tttgagctaa aaatccttca ttgatcattt gatcgttttc acctgggata
     2641 tgaggtacat gaatactgac aatatcgctt ttggctaata actcttcaag cgagtggaac
     2701 gtcaatactt ttttagcagc ttctgtctga tgaatatagt agccgatcac tttggctcct
     2761 agtcccttaa acaaaacagc ttccgtcaac cctatcctgc ctgttcctat gatccctact
     2821 gtgcaatttc tgatttcacg actaaacatt tgctcatcca ctcgaaaatc tttgaaagat
     2881 gttcggatcg tcgtatacgc agtatttctc aaaagcatca tggcaaaagt caaagataac
     2941 tctgctatcg catttggcga atacgatggt acacgggcta ctgtcatacc aaaatcagct
     3001 gccgcttgta gatcgatatg attgactccc acagtccgtg taaaaacata acgaatccca
     3061 tattcctgca tttttgcaag attggtctca tctgctatac aatttcccct caacaataca
     3121 gcatcatggt cctttgctga cacgatattg tcatgtgtca acagttcttc ataaagactg
     3181 agatcataat ggtaaatatt caatttttca aaaaaatcta tctcgttcgg acgaacaccg
     3241 taacatgcga ttctcatact ttcttcctcc atttcttatg aaaataaacc tatgccatga
     3301 atcatttgta atacaacgat ccatacaggt gtcagtacca cagcaaaaat agttgacagt
     3361 aaggatgcat tggaggctaa aagtgcctcc ctatcaaaac tgatagcata ggctgctgcc
     3421 actgttgcag ctggtgtagc catcatgata acgattgttg ccagtgcttc aaaattgact
     3481 ggtagaatat tcgttgccgt caaaactgct agaatcacga tattcaatgc aggtacaaat
     3541 accactttga ctagactgta ataccatgaa gttttttcag atgcagcttt tttgaagctt
     3601 acttcaccta atgttgcgcc gattgctagc cacgccaatg gtgaacttaa accagctaaa
     3661 taatccatcg gctgaaatag ccacggtgca gtctggtcaa ttcgcaaaaa cgctacttgg
     3721 cttatctctc cttctgcacc tgtaactgac acttgtggca gatacccttg gaacagccaa
     3781 atgaaaaggc cggcaaaagt agcgataatg attggattga cgaacatcgc tttgatattt
     3841 ttagcttcca tcttcaagcc actcattttt atgtatccat aagaatacaa aaagattcgg
     3901 tagccaatat tgaaaattga ggaatacatc acgccgacag gaccataaat cgcactgacg
     3961 ataggcgttc cgaaaaaggt cgtagaacca aagatcgtca ggacacgcaa cgttgtttgc
     4021 ttatcccctt tatagcttaa gaaaaaaggt tttgaaacta gaataagaat aatataaata
     4081 actaatcccc aaatcaagac attgattcct tgcatcagta attctgtatt gatatcttgc
     4141 atgaaagctc gaaatgccag tgctggcaaa gccactgtta agacgacttt cgatagaatt
     4201 tttccaacag attcagaaaa aatccccttt tttctacaaa caaaccctaa taatataata
     4261 aaaactgttg aagtgattgc actgacaatc gacatatctg tcaaagtcga tcgaaaaatg
     4321 tcaaataagt tcatctttct cctcctaatt atgttcattt attcacaata aaaaaagctt
     4381 ttattttgct aatacgtatt aacattttta attttatctt tacattgtta cttattgaac
     4441 aataggaaat taaagaaact tttgaaactt atgtaaagtg attttt
//
LOCUS       DAYLZO010000175         4477 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.181, whole genome shotgun sequence.
ACCESSION   DAYLZO010000175 DAYLZO010000000
VERSION     DAYLZO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.181"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            598..744
                     /locus_tag="ACGVJG_002874"
                     /pseudo
     CDS             598..744
                     /locus_tag="ACGVJG_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(832..1125)
                     /locus_tag="ACGVJG_002875"
     CDS             complement(832..1125)
                     /locus_tag="ACGVJG_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFJ6558769.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1162..1347
                     /locus_tag="ACGVJG_002876"
     CDS             1162..1347
                     /locus_tag="ACGVJG_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558770.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1528..2706)
                     /locus_tag="ACGVJG_002877"
     CDS             complement(1528..2706)
                     /locus_tag="ACGVJG_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6558771.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2810..3124)
                     /locus_tag="ACGVJG_002878"
     CDS             complement(2810..3124)
                     /locus_tag="ACGVJG_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558772.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3231..3446
                     /locus_tag="ACGVJG_002879"
     CDS             3231..3446
                     /locus_tag="ACGVJG_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558773.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3746..3988)
                     /locus_tag="ACGVJG_002880"
     CDS             complement(3746..3988)
                     /locus_tag="ACGVJG_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6558774.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3975..4403)
                     /locus_tag="ACGVJG_002881"
     CDS             complement(3975..4403)
                     /locus_tag="ACGVJG_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFJ6558775.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1418 a          841 c          663 g         1555 t
ORIGIN      
        1 ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt attattttat
       61 tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat tttagtcttg
      121 tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac gattattgaa
      181 aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct atgagggaag
      241 aataaaagag ttaggacggg attatctact atttttcttt gttttaaata tttgttatct
      301 ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac gaataaacgg
      361 tgttcaatta gctgatcttg tattcactta aaaaatggta aagtacgagt tgctattccc
      421 cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca ttggaataaa
      481 agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca tctgtccaaa
      541 atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag aattatcttt
      601 attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat tttactgacg
      661 attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca tattttgaat
      721 aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt atgcggtgta
      781 gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact tctaattttc
      841 aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc caaatgtgat
      901 tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca gtatcgaatt
      961 aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt ctttcaatag
     1021 gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat tcatgaatgt
     1081 gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct ttctcctttt
     1141 gattctaaga atactccatt tatgttagaa tattctggat tttttattaa agcggttact
     1201 cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg tagatttatt
     1261 ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg gtgttcaaat
     1321 tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc tgataacaat
     1381 gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga tataaaagga
     1441 tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt tcaagaatcc
     1501 tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag caatcagagg
     1561 ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt ggtcagtatg
     1621 gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc ttgccattgt
     1681 atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca cgcaccgttt
     1741 gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag aatcaagata
     1801 cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct cacccaaagc
     1861 cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc gtctagcaga
     1921 actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa ctgccgtttg
     1981 cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta gagcaagcgc
     2041 ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca tcggtaattc
     2101 ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa gaaacttgtc
     2161 ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt ttggcattct
     2221 cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt taaaaatcat
     2281 atttagaact tgaataagtg attgtaaact atttttggca tagccaacat ttgttccatt
     2341 aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg tcaaatcaga
     2401 caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc gcttatcata
     2461 ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct cacctacaac
     2521 actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt tctccatttc
     2581 cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg ttcgatatgc
     2641 ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc gatctgtttt
     2701 ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc ctttggaaat
     2761 ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact tataaagatc
     2821 gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc cattctaaaa
     2881 catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac tcaccaagtt
     2941 ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa tcagtatttg
     3001 ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct tccagttttt
     3061 tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta acttccaact
     3121 ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa aattgtaaaa
     3181 aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat atgaaacaag
     3241 gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct aatgcaatag
     3301 cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga gagagagctg
     3361 aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg aaaaatatgc
     3421 caaccaaaat cgaaatatgg aaatagtagt ttttctgatc tatcagctgt tttgccaaag
     3481 cacataggaa tctcacctcc ccccagtaat ggcgggccga tttggttacg gaagtatcat
     3541 tatcgtagtc gcgattctca tattaaaatt tgtcgcttaa aagctaacct tgtcatggcg
     3601 taactagaga atcaaaatct acatgtactg atagatttgc tattcatttg tcaaagagcg
     3661 ctaggctatt ttcagcctat ggaaatcctt tattttgacc cgataaaaaa cttccataga
     3721 atgaaaagga aagggaaaaa atactttaac gagttgtatc aaattcaagt atcttctcaa
     3781 tcaattttgt ttccagttgt ctacgcatat attcatctac atacataacc attccttttt
     3841 cttcttcata aactggtttc atggctaacc gtgaaatata attttgatat tttgacagaa
     3901 cgatggacat ggcaattgaa tcaccagaag ctgcggcttt gattgtcgtt agtggtatga
     3961 atggtatata tcgtttattt tgcttcatat ttgacaccca aatactttcc tagctcttcc
     4021 aaagccattt gtcgttggta ccaaacactg ccgagacttt tcccaataat atcgccaatt
     4081 tcacgatcat taagaccgat gaagaaggac ataaaaatga tttcttgtct ttgactaggt
     4141 aatgctgcaa tcgcatttgc caactcctca ttggaaataa agattttcat accaaggaca
     4201 ataaaagtac cactactatt caaatttggt tccaaataat agaactctgt acctaaccct
     4261 ttttgttcca atatttccat tgatatttgc cgttggcgaa atctagcgta cttcttttga
     4321 atatttaacg cctcattgca gactactttc ttacaaaaac tgtcaaacgt atgttgaatg
     4381 tgggtttcct ctgagtcgcc cataatattc tccccctcaa aaaattcatt caactcgttt
     4441 tacttctatc cctttccatt attactacaa tctagga
//
LOCUS       DAYLZO010000176         4433 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.182, whole genome shotgun sequence.
ACCESSION   DAYLZO010000176 DAYLZO010000000
VERSION     DAYLZO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.182"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(543..2048)
                     /locus_tag="ACGVJG_002882"
     CDS             complement(543..2048)
                     /locus_tag="ACGVJG_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6558776.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACGVJG_002883"
     CDS             complement(2045..2731)
                     /locus_tag="ACGVJG_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFJ6558777.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACGVJG_002884"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACGVJG_002884"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFJ6558778.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAYLZO010000177         4316 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.183, whole genome shotgun sequence.
ACCESSION   DAYLZO010000177 DAYLZO010000000
VERSION     DAYLZO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.183"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            148..1137
                     /locus_tag="ACGVJG_002885"
     CDS             148..1137
                     /locus_tag="ACGVJG_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFJ6558779.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="ACGVJG_002886"
     CDS             1134..1553
                     /locus_tag="ACGVJG_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558780.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="ACGVJG_002887"
     CDS             1562..1870
                     /locus_tag="ACGVJG_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558781.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="ACGVJG_002888"
     CDS             1874..2236
                     /locus_tag="ACGVJG_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558782.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNEYDGLLGVIPSKK
                     SQMNESKFMSIFNDVNLR"
     gene            2458..3864
                     /locus_tag="ACGVJG_002889"
     CDS             2458..3864
                     /locus_tag="ACGVJG_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFJ6558783.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELGYISRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKKAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            complement(4081..>4316)
                     /locus_tag="ACGVJG_002890"
     CDS             complement(4081..>4316)
                     /locus_tag="ACGVJG_002890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558784.1"
                     /translation="SSLEPQGIDPYYSPDFFAEIVAYSIKIRRNIKKRGRNRPLFSRF
                     KPQNSKTHFCIKFGQLTTAFLREGSNFDTLTAC"
BASE COUNT         1505 a          605 c          988 g         1218 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gttatcgtca atttctgacg atcagcaaca cgaaaaatca gtggataatg agtggcttgc
     2101 tgatgaaaaa tcagcgccaa gcgaacgaga ttatcccaat gaatatgacg gcttactcgg
     2161 agtgataccg agtaaaaaaa gtcagatgaa tgagagtaaa tttatgtcaa tttttaatga
     2221 cgtgaattta cgataagcta ttattttgcg tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacagcga aatccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtctaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaacaaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat cgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaattg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tgttcacgaa
     3181 tataaagaaa tcatgagcga gatttcagaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttggg gtatatttct aggcaaagag aagaaaacga gcgagaaaag
     3301 gctctggtgg cttctgagag agcaaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatattgttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctacat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaaagcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttggtct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtctg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacctag cgttagcgaa agtttggggg cgcccttttc
     4021 aaagggagcg gttttatttt tgcatgacta agaatgagtg ggaaaaactt aaccgcattt
     4081 ctaacaggcg gttaaagtat caaagtttga cccttccctt aaaaatgcag tggtcaattg
     4141 accgaatttt atgcagaagt gggtttttga gttttggggt ttgaaacgag aaaaaagagg
     4201 tcgatttcga cctctttttt tgatgtttct tcttatcttg atactatagg caactatttc
     4261 tgcgaagaaa tcggggctat agtatgggtc tatcccttgt ggctctaagg aagaac
//
LOCUS       DAYLZO010000178         4312 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.184, whole genome shotgun sequence.
ACCESSION   DAYLZO010000178 DAYLZO010000000
VERSION     DAYLZO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.184"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            71..154
                     /locus_tag="ACGVJG_002891"
     CDS             71..154
                     /locus_tag="ACGVJG_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFJ6558785.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            279..1016
                     /gene="erm(B)"
                     /locus_tag="ACGVJG_002892"
     CDS             279..1016
                     /gene="erm(B)"
                     /locus_tag="ACGVJG_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFJ6558786.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1186..1446
                     /locus_tag="ACGVJG_002893"
                     /pseudo
     CDS             1186..1446
                     /locus_tag="ACGVJG_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1609..2274
                     /locus_tag="ACGVJG_002894"
                     /pseudo
     CDS             1609..2274
                     /locus_tag="ACGVJG_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2310..2555
                     /locus_tag="ACGVJG_002895"
     CDS             2310..2555
                     /locus_tag="ACGVJG_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFJ6558787.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2658..3527
                     /locus_tag="ACGVJG_002896"
     CDS             2658..3527
                     /locus_tag="ACGVJG_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFJ6558788.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3508..4242
                     /locus_tag="ACGVJG_002897"
     CDS             3508..4242
                     /locus_tag="ACGVJG_002897"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFJ6558789.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
BASE COUNT         1547 a          635 c          837 g         1293 t
ORIGIN      
        1 ttttgtgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg taatgtagat aaaacatcta ctgttttgaa
      121 acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt cctaccagtg
      181 actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt ttaacttctg
      241 tatttattta cagatgtaat aacttcagga gtgattacat gaacaaaaat ataaaatatt
      301 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
      361 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
      421 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
      481 cagaaaaatt aaaactgaac attcgtgtca ctttaattca ccaagatatt ctacagtttc
      541 aattccctaa caaacagagg tataaaattg ttgggagtat tccttaccat ttaagcacac
      601 aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag
      661 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
      721 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag
      781 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
      841 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
      901 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt
      961 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
     1021 atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg agataaatta
     1081 ttagatatac tactgacagc ttccaagaag ctaaagaggt ccctagcgcc tacggggaat
     1141 ttgtatcgat aaggggtaca aattcccact aagcgctcgg gaccccttgt aggaaaatgt
     1201 cctaagtgtg gcaacaatat tgtattaaaa aaatcgtttt atggttgttc aaattatcct
     1261 gaatgtaagt ttactttagc tgaacatttt agaaagaaaa aactaaccaa aacgaatgta
     1321 aaagaattac tggagggaaa agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag
     1381 ccctacaatg ccgttgtaaa aattggggaa aagggatata ttgattttat ctctttttca
     1441 aaataaacat ggggctgacc caaaagtagc tcctatatga aaaatagagg tctttccatt
     1501 tagatagtag tggaaagacc tctatttatc ttttgaaagt gaatacaaaa ataaagcaca
     1561 ctctgctata atataggtga ttaatccatt tgagagaggt gctttatcat gtatcaaaat
     1621 tataccataa accaactttg tttgcctata gatcttgaaa ttaaattaga agaaaatgat
     1681 tttgctcatg ccattgtgca gtttgttgac tctattcccg atgaagtctt tcttccttat
     1741 tatcaatcta tgggtagacc ccaatatcat cctcgtatgc tactttccat tattctttgt
     1801 gcctatattc aaggggttta ttcgggccgc caaatacaaa atatgctgat agatagtatt
     1861 cggatgcgct acttatctca agagcaattc cctaactttc gtactattaa tcgttttcgc
     1921 gttcatccga ttatgaacga tattctagat cattcatttg ttcaatttcg tgagctactg
     1981 gttcaaagtg gcttaatcag tggcagtgct ctatacattg atggtacaaa aattgaagcg
     2041 gatgcgaata aatactcttt cgtttggaaa aaaagtattt taaagtataa ggatagcctt
     2101 gatcaaaaag cccttgaaaa ctaccaacaa atggtggaaa caaaaatctt acctgagtta
     2161 atagatgatt taaaggatga actttctata gaagagattg aaaggattcg tcaatctttg
     2221 gaagaaaagg aaaatcaatt aattgaagcg attgaccaaa ccgaaacagt agaagggggc
     2281 tgacccatta ttaataagga gtggacaaca tgattgatgt aaatgagcta ttggaagaag
     2341 ctattagaga aactgaaaat ctaaatgatg gcgaagtttt tcttgttaaa gatttattca
     2401 agggatatgt ggggaatagg atacccagaa aggatcgact tctgcttgga acgttatttt
     2461 taaaccatgt tagcaaaatg aatggtaatt taaaggctat tgaaaagact tcatctaatc
     2521 agcagaggta taaaaagaca attgataagt tttaactatc ttttgtgaaa ttaaaaaaat
     2581 tataaatatg ttgagtagaa gcaaaagatt tggcttggaa tttttacagc agtgaatcga
     2641 taaaaggagc atgcttgatg gtagataata ttattaaatc agtagcagag aaattatcct
     2701 ctctgtctta tatagaaggt attgttttag gtggttcacg tgcaaggggc acccatacag
     2761 aggattcgga tatagatatc ggcatctatt acaattcaga atcatttgac ataaatacta
     2821 ttaatcaatt cgctacaaag ctggatgatg agcatagaaa taaccttgtt gtacctcccg
     2881 gagcatgggg tgattggatt aatggcggcg gatggttagt cataaacggg tatcatgtgg
     2941 atttaatttt acgtgatatt aaacgtgtgg aacaaataat gaaagataca gagcacggaa
     3001 ttgttactgc caattatcag actgggcatc cccatggtta tattagtgca atgtatcgag
     3061 gagaattagc gattagcaaa atactatatg ctaagaatga aagcttatgc gaattaaaaa
     3121 aacaggcaga aacttatccc aatgctttgc agaaaagttt agttaacttt tttatgtttg
     3181 aagcagggtt ctctttaatg tttgtaaaag caaattcggg aacagacgat aaatattata
     3241 ttgcgggtca tgtttttcgt atagtttcat gtttaaatca agtgttattt gcatgtaata
     3301 atgcttattg tatcaacgaa aagaaagcta taaaactgct tgaaactttt gaacataaac
     3361 ctgaaaaata taccgagaag gtaaatcata tttttgaagt actcggtatc tcactttttg
     3421 aatgctacga catgaccgag aagctttata atgaagtgaa tgaaattgta tcggagataa
     3481 ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt
     3541 tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg
     3601 gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc
     3661 ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt
     3721 gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa
     3781 tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta
     3841 agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg
     3901 atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat
     3961 ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat
     4021 tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat
     4081 agaacactga ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt
     4141 gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga
     4201 cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa
     4261 cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tc
//
LOCUS       DAYLZO010000179         4287 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.185, whole genome shotgun sequence.
ACCESSION   DAYLZO010000179 DAYLZO010000000
VERSION     DAYLZO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.185"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..227
                     /locus_tag="ACGVJG_002898"
     CDS             <1..227
                     /locus_tag="ACGVJG_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6558790.1"
                     /translation="FIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYL
                     SKKLVEGMGGEMTARLDGGIFSISVKLRRV"
     gene            422..2071
                     /locus_tag="ACGVJG_002899"
     CDS             422..2071
                     /locus_tag="ACGVJG_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFJ6558791.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            2665..3600
                     /locus_tag="ACGVJG_002900"
     CDS             2665..3600
                     /locus_tag="ACGVJG_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558792.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            3593..>4287
                     /locus_tag="ACGVJG_002901"
     CDS             3593..>4287
                     /locus_tag="ACGVJG_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558793.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIV"
BASE COUNT         1338 a          742 c          939 g         1268 t
ORIGIN      
        1 cattcattcg attaacagtg gccaatccta tcaaaccagg agcgcaaacc tctgttgaaa
       61 aagtcttcca gcgtttttat accgaaaata ccagccgcac aaatacggaa tctagcggaa
      121 tcgggcttta cctgtcaaaa aaattggtgg aaggaatggg aggagagatg accgctagat
      181 tagacggggg catcttctcc atctccgtca agttgcggag ggtttaattg gctctaattt
      241 acctgaagtg aatgacaaaa aattgtatct cgttttaaag acatcttctt ttttggaagg
      301 tgtttttatt ttggtgtgat tgatacgaat taggtaacga agacagttat ttttgtaccc
      361 ccttttcaaa tagcgcaaca gtgctatact aaaactaaca aatcaaggaa cggagaggga
      421 tatgggccaa agagaattta tcgttttaat tattattgca gttgttattt tattagttgt
      481 tttagacatt ttttcacgtt tgaaagtaaa ggaaacagtt cgtagtaatt ggggaaaaat
      541 tccttatcaa ccgagatttg ataaagaaga aagtttgaag gatgcgtggc tcacggagaa
      601 aaaattccgt tcatgggata gtgaaatcga cgatcttact tggtatgact tggatatgtt
      661 cgaagtattt gaagggatca acagtaccta ttctagtgta ggttcagaag cgttgtatca
      721 gcgtttacgt agctttgatt ttggagaaga ttatcagtta gaaaaactaa ttgcttttta
      781 tcaggaaaat cctcaactga gagaaagaat ccaatatcaa ttcgctcgtt taggaaaaaa
      841 agatcataat tttgcaaaac aatatctagc ggatggaaaa agtaaaaaga ttggacgcgt
      901 aggtttattt atttttcttg gcttgcttcc gctggtgggt gtaggtttgc ttcttttagg
      961 gcaagtacta ggaatctatc tagcaatcgg tggcttagta ttcaatacgg tctattatct
     1021 agtgaagaaa caaacactag aaacagagct gaatagcatg agttatttgg tttcaacgat
     1081 ttcagccgcc aatcagttag caaaaatgac gacaccactt caaaaagagc tgaaacagca
     1141 aactaaacca ttaagagaca tccctaaatt aggtttttca tttcgaatga agagcgggag
     1201 tgaagcagaa ttgatttttg aatatgtcaa catcatgttc atgctgccat ttatctccta
     1261 tcatttcgtt ctcactaaat tagaaaagca ttcaactgaa gccatcaagc tgtgggaatt
     1321 gctgggagaa ttggaagttg cagcagccgt tttgaattat cgcacctata tgccgatcac
     1381 ttgccgacca gagtttactg agggcggtgt gacagcgaaa gatatctatc atccattgtt
     1441 aaaagaagcg gtagttaatc cagtcaactg gcagaagaat acattagtca cgggttccaa
     1501 tgcatcagga aaatctacgt atgtcaaaag tatagccatc agctgtattc tagcacagac
     1561 gatccaaaca gcgattgcag aacagtttac cctgcaacct ggacatgtgt tgacttccat
     1621 ggcaatcgaa gatgatttat ttgaaggcga cagttatttt gtttcagaaa tcaaatcgat
     1681 caaacgtttg ctggaccaag tggcttccaa agaacgctgc tactgcttcg tagatgagat
     1741 tttaaaagga acaaatacga tcgaacggat tgcttcttcc gcttcagtcg ttcggtggct
     1801 tgcggagtat ccatctttag catttgtcgc cacacatgat atcgaattga cagagatttt
     1861 aaagaattat tgtgagaatg tccactttga agaacaagta acagaaggaa aaggaatcag
     1921 ttttgatttc aaattaaaag aaggaccttc aaggacccga aatgcgattg ccttgctgaa
     1981 agttttgaat tatcctaaat ccatagttga acaggcccaa aaagaaagtt tgttgttcga
     2041 tgaacagcgt cagtggtatc cgtttgatta actttatcaa acgaagcatg atgcgtgggt
     2101 ccaaaaagac gattatcaaa agaagtacat tgatgccaaa aaagtggcag aattcaatgg
     2161 gaacgagtct gctgtccaga tgatcttgga agatttagca aaagacgata caaaataaac
     2221 cacgctgtct tccttctagc agcttttatc gaaaaaactt agcatcagcg acgagacgca
     2281 cgttccatgg caaagttctc cgtaaaacaa acagcgaaaa cagaaattga aaataactat
     2341 ttccatttta tttatttcgg ttaaaataaa agaaaaaagc tttcgataaa aggacggagg
     2401 aaagcttaac atgaataaaa aagacaagaa cctcagttat aaggatcttc tatatgtcat
     2461 cgtagtagtt gctgtcggta cttttatgat tttaaaagtt ccagagttgt ttagatagtt
     2521 attctatcga agatgatcat gaactagaaa acacatctcc tctttgttag caggtgtgtt
     2581 tttgtgtgga agccaagttg ttttttcatc tgctgtattc catgatacaa ttaagaatca
     2641 tgtctataag gagataggaa ataaatggca aatgcgcttg aaataaataa tttgaaaaag
     2701 atctatggaa gtggggtcga agcattgcgg ggaatcgatc tttcggtgaa agaaggagat
     2761 ttctatgcgc tattagggcc aaatggagct gggaagtcta caacgatcgg gattattact
     2821 tctttagtca ataaaacatc tggaaaagtg aatgtcttcg gttatgatct ggacaaggat
     2881 ctagttcgag caaagcaaca gattggattg gttccacaag agttcaattt taaccctttt
     2941 gaaacggtcc agcaaatcgt cgtgaatcaa gcaggctact acggtgtctc tcgcaaagaa
     3001 gcgatgaaac gcagcgaaaa atatttgaag cagtccaatc tttgggaaaa gagaaatgtc
     3061 cgtgcacgta tgctttccgg gggaatgaag cgaagactga tgattgctag ggcattgatg
     3121 catgaaccac gtttgctgat cttggatgaa ccaacagcag gagttgacat cgagttgaga
     3181 cgcgagatgt gggagtttct acgagaattg aacgaaaatg gtacaacgat cattttgacg
     3241 acgcattatc tagaagaagc tgaaatgctt tgtcgcaaca tcggaatcat ccaatcgggt
     3301 gaactgatcg agaatacaag tatgaaagag cttctgtcaa aactgcaatt tgaaacattt
     3361 atctttgatt tagcgccata tgataccaaa cctgtgattg aaggatatgc ttgtacattt
     3421 gaagatgagc agacaatcgc tgtcgaagtt gaacgaaatc aaggggtgaa cggaatcttc
     3481 gatcagctta caagacaagg aatcaaagtt ctttctatga ggaataagtc gaatcgttta
     3541 gaagaactgt tcttgaaaat cacagaagaa aataatcata cggaggagaa aaatgtttaa
     3601 tctttatttt actgcattaa aaagtttagc tatcaaggaa acgaaccgct atttgcgtat
     3661 atgggtccaa acactggttc cgccagtgat cacgacatcc ttgtattttg ttattttcgg
     3721 aaaaatgatc ggtgggcgga tcggtgacat gggtggtttt tcatatatgg aattcatcgt
     3781 tcctggtttg attatgatgt ctgctatcac tagttcgtat gccaatgttt cttcgtcctt
     3841 cttctctcaa aaatttcaga aaaatatcga agaaatcttg gtagcacctg ttcctacgca
     3901 tgtgattatt tggggctttg tgattggtgg tgtgggtcgc agtattctcg tcggctctct
     3961 cgtaacgatc atttccttgt tctttgtacc acttcatgtg tattcatggt tcatggtcat
     4021 cattacatta ttgatgacgg caatcttgtt ttcattagca ggactgctca acgggatttt
     4081 tgctcagtct tatgatgatg tatcgatcgt gcctacattt gtcttgcagc cgttaactta
     4141 tctaggtggc gttttctatg ccatctctat gttaccgccc ttttggcaag ctgtttcaaa
     4201 agtcaatccg atcgtttata tgatctccgg tttccgttac ggattccttg gaacgatcga
     4261 tgtgccagta gcaacatcga tgatcgt
//
LOCUS       DAYLZO010000180         4283 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.186, whole genome shotgun sequence.
ACCESSION   DAYLZO010000180 DAYLZO010000000
VERSION     DAYLZO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.186"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..237
                     /locus_tag="ACGVJG_002902"
     CDS             <1..237
                     /locus_tag="ACGVJG_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFJ6558794.1"
                     /translation="IYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSA
                     ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(490..639)
                     /locus_tag="ACGVJG_002903"
     CDS             complement(490..639)
                     /locus_tag="ACGVJG_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558795.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            1101..1313
                     /locus_tag="ACGVJG_002904"
     CDS             1101..1313
                     /locus_tag="ACGVJG_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558796.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1591..3390)
                     /locus_tag="ACGVJG_002905"
     CDS             complement(1591..3390)
                     /locus_tag="ACGVJG_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFJ6558797.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3514..4110)
                     /locus_tag="ACGVJG_002906"
     CDS             complement(3514..4110)
                     /locus_tag="ACGVJG_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFJ6558798.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1529 a          749 c          733 g         1272 t
ORIGIN      
        1 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
       61 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      121 attcaatctg ccgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaaca
      181 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
      241 ttaagattat attaaatatt atacagataa taacggaata ttatcatcga aacagctaac
      301 aaaattgtca taaaaaacga gatggttgat attagaactt gtgtttttgt ttaggtctag
      361 agataaagag agacaatttc caaatatatg taatgaatac aaaaagacgt ttgtcgcgga
      421 ttaccgttac aaacgtcttt tattgatttt ccgagatctc atcagtcgcc atttggaatg
      481 gttcactcct tatctagatt cgtttaatgc tcggactgct tgttcgattg taataccaaa
      541 cgcaacgtta ttttcgtttt gtgcatcaat cgccataaaa cggttagaag ttgtatcaaa
      601 agctacgcgg tattgcatat ttgtttttgt aaacgtcata aaattcatct cctttgtaat
      661 gtaatttagt tcttgaaagt ttcctttaaa ctttcgaact tataccaagt ttagcatcgt
      721 acgttttttg atgcaattaa aaagcattta tcattttcct tatttatttt ttcttaaatg
      781 gtttagctat tttcttttaa tccgataagt agcccaatca tcaaggttac aaatactatt
      841 gcttgaatat aaatcatatg ctcacctctt tataaataac ttagcaatat aaagaaaaaa
      901 acgcacgaac aacataggaa tataaacaaa cgtttttgtc acaacgtcta agatcgtgtt
      961 aaaaaatagt tatctaaaaa gcagaaagat actcaaaata aaaaataaaa aaatggataa
     1021 aaacgttttc aaatacaagg taatttgtta ttataaaata aagaaaaaac aaaataaaaa
     1081 taaagaaaag agggaatgaa atggtaaaag cagaagtagg aagatcttat atatgcaaac
     1141 cgatcggagt atctcatgaa ctaatcggct gtattgaacg tacatatatc aacacggctg
     1201 tcgtgtatgt tgaaagttat gaccaacgag actatcaact tatcaatgaa agcaagtatc
     1261 gcatgctcgt aaaattctgt gatattagcg cacctgctga attggtttct taaacagtat
     1321 ttttgagaat tcgcaagaac tcccttataa tataaataaa gacagaaggg agcgagaaga
     1381 tatgccaaaa agaaaaagtt cagggaaaat gggaattatt cttgcagtga tctctgcaat
     1441 tcctgctatt ttagctattg ttcgtgcgat gaaggaatca agagaaagtt aagcacacat
     1501 tatgagcgtc aaacaaaaaa cgcatatacc tgcggtggca ggttatgcgt tttttgttgt
     1561 ataaagactt ttttcattaa cgatttttga ttagccgact cccaaaacaa aatttagcag
     1621 tttatctgaa taagtgattt cttcatctgt ttgtttcaca ctttcattta gtagtcccat
     1681 ctcatcggtt atgataccat ccacaccata aaacatcatg cgactcatag catctgaatc
     1741 gttgattgtc catacatata cttcttttcc gtcttggtgg gcagaattaa tgaagttacg
     1801 gttgatcgta ctatattcca ttgtcagaaa atcagcaggc gtcacagggg gcccaacaat
     1861 attgaaaggt aagatgtagc ccacataaaa gtttggttct gcttctttta aatctgaaac
     1921 ggtttggtac gtcaaacttt gcaaaatatg atggttctct aagataactt tgcggtattt
     1981 atggacaaat cgttcgacaa ggtcatccga atctttttta gaggtcttga tctcaataag
     2041 taatttttgg tttagcttat gtgctttttc caaataagaa tcaaaagaac aaacaggggc
     2101 ttgcactccg ttttcagtaa ctgtaagatt tgtcaattct ttcaatgtaa gatcctgtgg
     2161 tgttccattt acccctgtta agtgacgaag atttgcatca tgcatcacta cgaaatgacc
     2221 atcttttgtt tcctgaacat ccatctctac ataatcagga tgatagtctc tgcttgtttt
     2281 ttcaagtgct tctaaagtat tttgcgcccc attttgggta ctcacacctc tgtgagagac
     2341 agtcactggt tttgtctgaa cagctgaagt cagataatcc atattgtaaa gacagacgcc
     2401 aataccgaaa aaaactgcaa aaagagtcaa accagtgttt ttcagcgcag agaaacgcaa
     2461 gttgggggct tgtgggataa accacttagg gatctcaggc aagaagcctt cgtcatccat
     2521 gaaatcaaca atgatataaa aaatccctac ggtagacatc acgatattga ataataaaat
     2581 cccttgtaaa agcgtcatag caaacacagc gctgatcaat gcatagtctg ggaaaaattg
     2641 ttcaaccata ctttgtgcca aaataacaac gatatatcct aaagaagaaa ggagtaaaat
     2701 cgtaccgcca atcacaataa attggcccgt gatggccaaa agacgcgatt tggtcaatga
     2761 ccagctttcc cgaatggctg ctcgaaaagg acgatctcgt agaatcattt ccggtaaagc
     2821 aaaaatcagt cgaatcccaa tatagccaag gaaaatataa acgagtaaaa aggaactgac
     2881 gatgatccaa cgattagcaa aaataaaatc cataatgaaa gcggggattt ttatttttga
     2941 cagtagatca gaattaaaac ttaatccgct gatcggtaga atcaataaaa aataagcaag
     3001 gaaaaacaaa aaagtaatgg gtctcacttt cttcagctga agtatcgtca agtgtaagag
     3061 ttgcttcaaa gagattggtt ctttcttttt aataaaaaaa acactcatca aaagaaaagt
     3121 aaattcgaaa taaactaaca gtaaaatcgt catcaaaact acgattaacg agagtagaac
     3181 cccaggatgt tgagaaaaaa ttgaaggaat attgtcataa gacaaatagt caattgcccc
     3241 tcggtgtaaa ataaaacggg tcgtactgct caataagggt aaacaaataa ataaaataaa
     3301 gccatgcatc agcaagacat cacggaaata agcttgtgtt ccttttaaaa agtcccaagc
     3361 attacctaag ctacgtttca aataattcat cctatcacct gctcttcaca ttgtagcgca
     3421 aatctaatga aaaaggaaag ggtgcagtgt gttttgcaga ccctttccca gatttaacat
     3481 tgtcaatcaa tgactaattt tacaaaataa atattatcca agaagaccac ttagatattg
     3541 acctccataa atcaaggcca tactatataa gaaaatcgac agtggtttag caatcaaaat
     3601 gattagtgta tatcgtttca ctgacatgtt tgtcaatcca gccatcaaac ataacgcatc
     3661 gtcaggagca actggaagaa aaatagctag agcaaacagc ttctcaaaac ggttttgatt
     3721 atccagccag ccaatatatt tgttatatgt cttatcgcta atcatggaaa gtatgaatgg
     3781 ctttccataa cgacgcccta gcagaaaaat gataatcgaa ccaatgcaga ttccaatata
     3841 gttatagata aatccagcat aagggccaaa tatcaggaca ccagctgcag tactgatccc
     3901 tccaggaatg attggaataa ctacttgcag gatttggatg aagacaaaaa taatcggtcc
     3961 taaaatgatg gaatcaccta ctaatcctct taacgcattc aaatctttga aaacacctaa
     4021 attaataaaa taaatcgtca gcgcgattga taggcctaat ccgataatgg atatgaaatt
     4081 tatcagtttt cgagaggcag caatattcat tattcatcaa ctccttagag gttctttgtt
     4141 aactatactt tactagaaaa gcaactaact gcgccatttt ttgatatgac agaaaacaaa
     4201 caattttgta agttcatcat aagtataaaa gattgatctc aaaaaaacat ccaactaaag
     4261 acttatttaa taacttgttt ttt
//
LOCUS       DAYLZO010000181         4094 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.187, whole genome shotgun sequence.
ACCESSION   DAYLZO010000181 DAYLZO010000000
VERSION     DAYLZO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.187"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            360..1328
                     /locus_tag="ACGVJG_002907"
     CDS             360..1328
                     /locus_tag="ACGVJG_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFJ6558799.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1396..2154
                     /locus_tag="ACGVJG_002908"
     CDS             1396..2154
                     /locus_tag="ACGVJG_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFJ6558800.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKKIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2222..2932
                     /locus_tag="ACGVJG_002909"
     CDS             2222..2932
                     /locus_tag="ACGVJG_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6558801.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2968..>4094
                     /locus_tag="ACGVJG_002910"
     CDS             2968..>4094
                     /locus_tag="ACGVJG_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFJ6558802.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1362 a          617 c          879 g         1236 t
ORIGIN      
        1 tgcacccatc tacccgtcaa atcactttat tttccactcc agtagtttcc taattcaact
       61 gtgtgcaaaa cgatttcttc taaacaaatc aagacaaact tataaacctt catcccagca
      121 ccataattat ttaaatcata ggcatttttc gaaattagta tatgtactca agtttcactg
      181 cagattattt atatttttta gagaaataaa tttttcctat tgacaaacac aaagaaagcg
      241 gatacaatac aattatgtca catgacgtta tatgacgtta tgtaatatct ttacatttaa
      301 tcagttactt ttattttgga taaaattaac atttttaaaa ttttaaggag gaaatgttga
      361 tggatgtagc aaaaacggtt aacgcaattg tagaaaaaaa tgatggagag atttctggaa
      421 tttatttggt ggcttgtggc ggttcgctgg tagatatgta tgtctcagat tattttctta
      481 aagccgaagc atcgaagtgt attacaggct tatatacagc gaacgaattt aaccatgtaa
      541 ccccaaagca attagatgat aaatctgttg tcattctatg ttctcatgga ggaaatacag
      601 cagaaacggt aaaagcagga gaagtcgctc aagctagagg agctgtgact gttggtctga
      661 ctcataataa aactgcggat ttattaaagg tttctgatca tagtttcctt tacgaatggg
      721 gagatgatgc aaaaatagtc aataatccta tggccatcat tcttgattta actgtatctt
      781 tggtcaatgt taccgaaaca tttgaacatt acggtaaatt cagagaaggc atgaaggtta
      841 tcgataccgt aattgaaaat ggcattaagc aggtgcagga tcgtattaaa gtgtttgctg
      901 ataagtacaa agatgaaaca atgttttata tcttaggtag tggaccatca tatggacatg
      961 cttatggttt ttcaatttgc tctttgatgg aaatgcaatg gcttgattca acttctgttc
     1021 attctggaga attcttccat ggaccttttg aagtaacaga caaagatacg aattttattc
     1081 tcttgatgag cattggacgc actcgtgctt tagatgaacg ggcaaaacga ttccttgata
     1141 aatatgcaga aagagtagaa atcgttgatg caaaggaact aggattggat atgatatcag
     1201 atgaagtttc tgaattcttc aatcccattt tattctacag tattctttca gaatatcgtt
     1261 cagctttagc agatgcacgc aatcatgatt tagatgttcg tcgctatatg gggaaagtgg
     1321 agtactagga ggattaaaat gaaggtatta ggcttaggtg ataacgtcgt cgataaatat
     1381 gaaaatctgg gaattatgta tcctggagga aattcattga attttgcagt ttttgcaaaa
     1441 aaactgggac atgaaagtag ctttatggga gtttttggct cagatgagga ggcagctcat
     1501 gttttatcag caataaaaaa aataggactg gacaacagtc actctcgctt tgaaaaaggc
     1561 gagaatggct gtgccagagt gaagattgat gagggcgacc ggattttcct tggcagcaat
     1621 tcgggaggtg ttactcgtga atacccgatt cagcttactg aagaagacag agaatattta
     1681 aaccagtttg aattgattca tatgggatta tatagccatg tcaatcatct attagaagag
     1741 ttacaaaatg tttctggaaa gatttcttat gatttttctg acgactttac ggaagaagag
     1801 gttcagcggg caattgataa agttgatttt ggcttctttt ctatagaaga cttttccgat
     1861 gaagaagtaa agcagttttt gatgaagcat ttttgtgagc gaaatgatgt attgattgca
     1921 acccgaggag aaaaaacggc tattggttat gatggagcaa agttttatga agtgaacccc
     1981 gtgctagaag aaccagttga tacaatggca gcgggagatt ctttcttaac agctttccta
     2041 ctttattttc tcgaagggga cgatatagtc accgcgatga aaaaggggaa tgaatttgct
     2101 gggaagtctt gtctggttga cggttcgttc ggctttggta ttcattacga ttgagcttgt
     2161 tttaaattcc tgcatttgat aacctaaaga aagaaatccc ggtaaagtgg aggttaaaaa
     2221 aatgctggat acaaatgcac aattaccatt gtacttacaa ttaaaagaaa tattaaaaaa
     2281 taaaatcaat acaggggagt atcccgagaa tacgaaaatt cccgctgaat cagagctgat
     2341 agaaagttat gaggtcagcc gaatcacagt gagaaaagca gtagaagaac tggtagaaga
     2401 agggtaccta cttaaaaaac aagggaaagg aacttttgtc aaccggcata aagtttttcg
     2461 taaaattgaa tatgttatgg gtttcagtga gagttgtaaa tctaatggtt ttacaccgac
     2521 tagcattgtt ttggaacgca agatcattcc agcaactgaa gggatggcaa aaaaactcga
     2581 tatcgctcaa ggtgatgatg ttttatatat ccagagaaag cgaatggcgg ataacatccc
     2641 cattatgtta gaaaacaatt atttttctta tgaaagatat agtttcttaa tggaggaaga
     2701 gctgactgat tctttgtatg aagtacttaa agtccaaggt accttagcca tcaatcctgg
     2761 ggaaactgca cttgaaattg tcacagctga tgaccaaatt gccaaaataa tggaagtagc
     2821 tgttgggacg ccctttttct ttatggatac tttaatcaac gatcaaaaca acataccaat
     2881 tcatgtaggt aatcaatatt acctgggaga agtatatcgt ttttacttat gatttcctgt
     2941 tttgatttca aggagtagat tttatttatg aagcaagtat tgcggtcaaa aatgattttt
     3001 gatggagagc agccaccttt caaaggttat attgtaatca atgaagggat cattgaaaag
     3061 gtggaaaaca actggaatta cagtgatcta ttaaatgaaa acacggaatt aattgcttat
     3121 gatgatcaat tcgttatgcc agcaattcat gataatcatg tatttttcag tggttatttg
     3181 gctatgaatg ctggcataga tttaagcgat acatcttcag ctaacgaggc tgtagagcgt
     3241 attaaagctg taatgaacca taaaaaagca gatgaaccaa tttatgctca tggctgggat
     3301 gctgccctgt ggaaaactga cccatcagaa gagctattga atgaaataaa ccactttggt
     3361 ccaatcacag cgatcgacaa aaatcgcagc cattgttgga tgaatgctat tgctaagcag
     3421 acttatggtt ttacagaaaa agaaaccagt gctgaaagtc gagtattatt aattaaggaa
     3481 atgctagcaa acaaaaagct tttagcagag gtttacgctg aatttgaagc attactgctt
     3541 tcaaaaggtg ttatatcaat gaaagaaatc gtttttgatg acgcagaatt tctttctaat
     3601 atacctcaga ggaaaatact tagtaaccta tatgtgcagg cagtccaaga acctctacag
     3661 catgaacttc tatatgagta taaggaaaaa gttttccctg aaaaggtccg ctttggtgga
     3721 gtgaagatca tggtagatgg tgttgttgcg gatagaagtg gagatattta cggtgtttat
     3781 cagtcaggag tagcttcacc cgagattgac tatgaggcaa ttaaagcaga agttgctgca
     3841 ttcaatgaag tagagattcc ttgctgtttg acaacggaag gagataaagc gggttataaa
     3901 gctgcgtcaa ttcttgtcga atttggaaag cggttacctg aaggcgtttt taattcgatt
     3961 agtgatttgg aaatggttac tcaaaagaca gcagaaaaaa tgaacgaagg acaagtagta
     4021 gcagaggtat atccacaaat tctcggcttg aatcaaacgt tagaagaggc ttacatgtca
     4081 actgttataa aaaa
//
LOCUS       DAYLZO010000182         4067 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.188, whole genome shotgun sequence.
ACCESSION   DAYLZO010000182 DAYLZO010000000
VERSION     DAYLZO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.188"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(149..1250)
                     /gene="prfB"
                     /locus_tag="ACGVJG_002911"
     CDS             complement(join(149..1177,1179..1250))
                     /gene="prfB"
                     /locus_tag="ACGVJG_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFJ6558803.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(1376..3910)
                     /gene="secA"
                     /locus_tag="ACGVJG_002912"
     CDS             complement(1376..3910)
                     /gene="secA"
                     /locus_tag="ACGVJG_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFJ6558804.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT          994 a          910 c          719 g         1444 t
ORIGIN      
        1 gtaccatttg agtacttctt cattacatcc ttcatttcaa tcatggcgtt ctctccatat
       61 ctttttcagt ctttagctat tatagcatgg caatgtttca acatgcttgc aaaatcttac
      121 aattttattt caatttaaca atgggttctt attgtagatg catttttaaa tagccgtcaa
      181 taaataaatc aaggtcaccg tccataactg cttggacatt tcctgtctct acgtttgtcc
      241 gatggtcttt taccatagaa tagggatgga aaacataaga acggatctgt gaaccccagc
      301 cgatttcttt ttgttctcct cgaagtgcag ctgcttcttg ttctttcttt tccacttcta
      361 attgataaag cttagctttt aacatcccca tcgcttgctc acggtttttc aattgtgagc
      421 gttgtgcctg actagctact actgttcctg taggcaagtg agtgatccgt acagcagatt
      481 ctgttttatt gatgtgctga ccacctgcac cacttgctcg gtacgtatcg attttcaaat
      541 catctggatt gatttctata tcgatcgttt catcaagctc tggcatcaca tctaccgaac
      601 aaaaagaagt atgacgtcgt ttcgctgagt caaaaggcga aatccgtaca aggcgatgaa
      661 cacctttttc tgattttaga taaccaaaag cgttatgtcc tttaatcagc agggtcacac
      721 tcttgatccc tgcttcatca cctgcttgat aatctagtgt ttctacttga tagccatgct
      781 gctctgccca acgtgtatac atacgtaaaa gcatgctgcc ccagtcttgg gactccgttc
      841 cgccagcccc tggatgcaat tcgacgatcg cattgtttcg atcataaggt ccgtctaaca
      901 gcatagataa ttcatacgtc ttcatttttt catctaatgc attgatacgt gtttctgctt
      961 cttcttgcat tgctgcatca ggttcttctt gcagcatctc caccatcaat tcgatttctt
     1021 ctagttcttc ggcaagagaa tggaattggt cataggtttc tttattagcg ttattttcgc
     1081 tgatcacttt ttgtgcggct tcgctgtcat cccaaaaacc aggttcagcc atttttgctt
     1141 ctgcttccgc gatgtcttct tccagctgat ctaagtcaaa gagacctcct gaaacttgtg
     1201 accttttcct gcatttctgt caggagattt cgaatttcgg atatttccat gttttttcct
     1261 tctttcacta tacttttttc attatatcta gtctaacaga atcagcaaat aacagaaaga
     1321 aggtgagacg aattcttaaa aaaagaattt gtctcaccaa tcctgctgtc agaaattagt
     1381 ttgcattttt tccatgacag ttcttatatt ttttcccgct tccacaagga caaggatcat
     1441 ttcttccgac cttattcaca tggattggct gttttttcgc gctagtatta ctattttctt
     1501 tggcatctcc gtcttctgag tgagaagctg ttccttgaga aacctgttca cgctgtacgt
     1561 tttgacggat ctcagctttc ataaagattc gagtgacttc atactcgatg gcgcctacca
     1621 tatcttcaaa catcttatag ccttctgttt gatattcgac taatggattg ttctgtccat
     1681 aagcacgtaa gccgattgac tgacgtaatt gatccattgc atcaatatgg tctgtccatt
     1741 tagagtccac aacacgcaag atcacgactt tttcaaattc caacagttgc tctggaccat
     1801 ttagttgagc cgctttggta ttaaatactt cttgtgctcg ttcgtttaga tactctttga
     1861 tctcctgagc tgtttttcct tcgatatctg caagagtaat gctatcttca tggacaagag
     1921 tactgccggc aaaatcgatg atactttcca aattccaatt ttctttctct aattgtgtat
     1981 gcgcatctac tacacgactg attgtccgtt tgaccatgtt cattagtggc ttggataagc
     2041 ttttttcttc catgattact tcttgtcgct gtttatagat tacttcacgt tgttcacgca
     2101 tcacatcatc gtattgtaag acgtttttac gtgtgtcata gttatttcct tctacacgtt
     2161 tttgtgcaga ttctacttgt ttcgtcagca tcttgctttg gataacagca tcttcctcac
     2221 cgatcttcat ccgatctaag aaagctttga ttcgttctga accgaatcgt ttcatcaaat
     2281 catcttccaa tgacaagtag aactgtgaca taccagggtc tccttgacgt cctgcacgtc
     2341 ctcgaagctg gttatcgata cgacgagatt catgtcgttc tgtcccgata actgctagac
     2401 caccaagttc tcttacccca agtcctaact tgatatctgt tccccgacca gccatgttgg
     2461 tagcaatcgt cacagcacct ttttgaccag cattcatgat gatctctgct tctttaaagt
     2521 gatttttcgc attcagcact tcatgaggaa tacgttcttg attcagcata tctgataata
     2581 actcagatgt ttcaaccgca acagttccga ctaacacagg ttgacctttg cggtagcgat
     2641 ctttgatatc ttgcacgaca gcgtggaact tgctttgtaa tgttgggtaa agcaaatctg
     2701 gacgatcatc acggataacc ggacgatttg tcggaatctg gtatacttgg atgttataaa
     2761 tttctctgaa ttcttcttct tctgttttag ctgtaccagt cattcccgca agtttcttgt
     2821 acatacggaa atagttctgg aaagtgatcg tcgccatggt tttcgtttcg tcttcgatct
     2881 ctacgccttc tttagcttcg attgcttggt gaagtccatc tgaataacgg cgtccatcca
     2941 tgatccgacc agtgaactgg tcaacaatca gtacttttcc gtcttggaca acataatcga
     3001 tatctaaaag catgatatag ttggctctca atgcttgatc caagtgatgg gtcaatgcag
     3061 tattttcaat gtcgtataag ttatccaaac cgaatgtttc ttctgctttt tcgatccctg
     3121 cttcggttaa actgattgtt tttgattgta catcgatttt ataatcttct tcttctttca
     3181 aacgtttcac gaagttgtct gctcttgtat ataaagctgt ggatttttct gcttgccctg
     3241 aaatgatcag cggcgttctc gcttcatcaa tcaagattga gtcaacttca tcgacaatgg
     3301 cataattcag cggacgttgt accatttgat ggcggtaaac gaccatattg tctctcaagt
     3361 agtcaaatcc taattcatta ttcgtactat aagtgatatc acaattataa gcctcgcgtt
     3421 tttcatccga agattttgag ttgatattca accccactgt taatcctaag aaattataaa
     3481 gttcgcccat ttccgtcgag tcacgggttg ctaaatattc attgacggta actacgtgta
     3541 ccccttcgcc agtcagagca tttagatata ctggcatcgt tgcggtcaag gttttacctt
     3601 caccagtacg catttcaggg atatttccat cgtgaagcac gattccaccc atcaattgta
     3661 cgtggtatgg atataaacct aatacacgtt ttgctgcttc tcggacgact gcaaaagctt
     3721 ccggaagtaa ttgatctaat gtttctcctt tttggtatct gcccttgaat tcagcagttt
     3781 ttgcctgtaa ttgttcatca gttaatgctg ccatttggtc agcgtatgct tcaacttttt
     3841 ttgcaatgct atctaaacgt tttagttctt tcttatcgtt ttcgataagt gaacgaatca
     3901 aattggccat gcgttgtgta atctcctttt ccttctgtgt attatccaga ctatgtagac
     3961 tttacttttc attttatcat tagttataga taattgaaat atcttaagcg tattaaagta
     4021 agaacttcat aatttattca tatttcatct aaaagaggag gaaactc
//
LOCUS       DAYLZO010000183         4063 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.189, whole genome shotgun sequence.
ACCESSION   DAYLZO010000183 DAYLZO010000000
VERSION     DAYLZO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.189"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            82..1431
                     /locus_tag="ACGVJG_002913"
     CDS             82..1431
                     /locus_tag="ACGVJG_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFJ6558805.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1559..2149)
                     /locus_tag="ACGVJG_002914"
     CDS             complement(1559..2149)
                     /locus_tag="ACGVJG_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFJ6558806.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2296..3648
                     /gene="brnQ"
                     /locus_tag="ACGVJG_002915"
     CDS             2296..3648
                     /gene="brnQ"
                     /locus_tag="ACGVJG_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFJ6558807.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1169 a          744 c          789 g         1361 t
ORIGIN      
        1 ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta attaaacttt
       61 tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg actgaaaaat
      121 agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt tttaggttcc
      181 ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat tactggatgc
      241 atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac caataaccat
      301 tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat cactggctgg
      361 acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat cggaatgtat
      421 gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc attaggtctt
      481 cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt ttggtttgca
      541 ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat gatcctcacc
      601 cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca tggcggattc
      661 tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt tgcttttgct
      721 gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa agtgttgccg
      781 gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc tctttttgtc
      841 attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt tgtagaagta
      901 ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt attaagtgca
      961 gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg ttctttagcg
     1021 caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt gccagggaat
     1081 gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta cgtgatgcct
     1141 agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt tatctggagt
     1201 atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa agctcattca
     1261 tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat ggcctttgtg
     1321 tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac acctgtgtgg
     1381 ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta agaaagtagt
     1441 tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt gcttcttaaa
     1501 tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag cctcttttct
     1561 atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa aaaatccaaa
     1621 taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag acaccggcaa
     1681 tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac aaaactctca
     1741 cgacatggga atctggttta tttgctttca tgatttgcct catcaagata ccaaaagaaa
     1801 aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct tcagggttac
     1861 gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa aagatattcg
     1921 accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga tggttcaccc
     1981 agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt cggatgaccc
     2041 ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaaga actctcaaaa
     2101 caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg atgattcctc
     2161 ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca tcgttttata
     2221 tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt taaaaaaacc
     2281 gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt tataggctcc
     2341 atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca tttgggtcaa
     2401 gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg tatcggtctt
     2461 cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata tgaattagca
     2521 aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt agttatcgga
     2581 ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt agcaccattt
     2641 atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt ttttgtgctg
     2701 gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa gttcctgaat
     2761 cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa tccactaggt
     2821 tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt cacagggttt
     2881 actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat catcattgtc
     2941 acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga cacgatcaaa
     3001 tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc ttatttaggt
     3061 gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct ggctcagatt
     3121 gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt tatattagct
     3181 tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac agatttgttt
     3241 ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg tttagcggca
     3301 aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt catctatcca
     3361 ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca gcgtacggct
     3421 gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg gttgaacgct
     3481 tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt tgcggaaagc
     3541 tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat cggttttgtt
     3601 atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata aaagaggttg
     3661 tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc ttctcacatt
     3721 tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt attgagaggt
     3781 cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg gcttctcaca
     3841 tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt ttattgagcg
     3901 tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca tatactttgc
     3961 cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct tttataatat
     4021 agattttaga catatatatg atataaaaat agttttcgca aaa
//
LOCUS       DAYLZO010000184         3956 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.190, whole genome shotgun sequence.
ACCESSION   DAYLZO010000184 DAYLZO010000000
VERSION     DAYLZO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.190"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..156
                     /locus_tag="ACGVJG_002916"
     CDS             <1..156
                     /locus_tag="ACGVJG_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFJ6558808.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACGVJG_002917"
     CDS             175..1452
                     /locus_tag="ACGVJG_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558809.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="ACGVJG_002918"
     CDS             1462..2118
                     /locus_tag="ACGVJG_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558810.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="ACGVJG_002919"
     CDS             2118..3494
                     /locus_tag="ACGVJG_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558811.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="ACGVJG_002920"
     CDS             3624..>3956
                     /locus_tag="ACGVJG_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFJ6558812.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          425 c          694 g         1243 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagaaag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa acaattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt tgggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 gagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DAYLZO010000185         3839 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.191, whole genome shotgun sequence.
ACCESSION   DAYLZO010000185 DAYLZO010000000
VERSION     DAYLZO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.191"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..693)
                     /locus_tag="ACGVJG_002921"
     CDS             complement(<1..693)
                     /locus_tag="ACGVJG_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFJ6558813.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQ"
     gene            848..2407
                     /locus_tag="ACGVJG_002922"
     CDS             848..2407
                     /locus_tag="ACGVJG_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFJ6558814.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(2518..>3839)
                     /locus_tag="ACGVJG_002923"
     CDS             complement(2518..>3839)
                     /locus_tag="ACGVJG_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFJ6558815.1"
                     /translation="GVEIKVETNGSEGIKNRLTAEDIARAEGVIVAADKKVEMNRFDG
                     KELINRPVSDGIRKPEELINLALSGTAPVFHGDGKESSSEEGNADGTIGQRIYKDLMN
                     GVSHMLPFVIGGGIAIALSFMIDQFIGVPHDQLSNLGNYNQAASWFNQIGQAAFGFML
                     PVLAGFIASSIGDRPGLIVGFAAGALANTGGAGFLGALIGGFLAGYVIVFLRKLFKNL
                     PKSLDGIKTILFYPVFGLLITGFLMLLVNVPMKAINDALNGFLTSMSGSNAALLGALL
                     AGMMAADLGGPINKAAYVFGTATLATTVATGGSVVMASVMAGGMVPPLAIFVATRLFK
                     NKFSKTDQDAGLTNIVMGLSFVTEGSIPFAAADPIRAIPSFIIGSALTGGLVGAFGIK
                     LLAPHGGIFVVFLLSHPVMYLVFIAIGAVVSGVIYGLLRKAPAAAEA"
BASE COUNT         1072 a          805 c          746 g         1216 t
ORIGIN      
        1 ctgtgcgtga tttgtatctt ggtactcatc tacatgaata taatggaatt tgttttgata
       61 atatgtgagt gaatcaggat gttcttcaaa taatcgaatc gtattcatga tcaggtcgtc
      121 aaagtccata cattgattat attgaagctc tttttggtaa gcagcataac attttgccgc
      181 aatctcttcg taataactgc cttgcatttt tgcaaaatcc tgaggggtct gcaggctgtt
      241 ttttgcttga gagattgttc ctaatatact tcgaggatca tattttttcg gatcaatgtt
      301 cagttctttt agtatccgtt tcatcaaggt cagctgttca gaagaatcga tgattgtaaa
      361 gttccggtta tacccgataa aatcaacatc tcttcttaaa atacgcacac acatcgagtg
      421 aaaagtcgat acccagacat cttcgcctcc agatgcgaga atcgcattga cacgttcttt
      481 catttctttt gcagccttat tggtaaaagt aatcgctagg atgttccaag gattgacttc
      541 tttttcctca atcaaatagg caatgcggtg tgtcagcact ctcgtctttc cacttcctgc
      601 acctgccatt agcagtaatg gtccgtcagt gtgaagaaca gcttctttct gacgggggtt
      661 catcccgttt aataattcag ctttggatga cattaacaac atcctttctt ttatcaatct
      721 tgttaattat agcattttct tggctttcat taaactagct agttcaaaat catttgtttc
      781 tttttatgga tttttccttg tcaattcgtt gatttcgcgc taaattaaaa gaaagcgagg
      841 cgtttgaatg gaaaaaaaag gaaaaatcaa gaaattagat aagactcaga tatattttat
      901 tctaaaagga atatttgttg gactgctgac aggaatcgtc gttagcctgt ttcgattatc
      961 tatcgaaaag ctaagcgatt tagttcgaag tatctatgaa atgagtcggg aacagccgat
     1021 ttacttaatt ggcctagcta tttgctgcat ccttgcagca tttttcgtgg gttatctggt
     1081 caaaaatgaa ccggatatca agggaagcgg gattccacaa gtagagggac agcttcgcgg
     1141 ggaattatca atgaactggt tctctgtttt atggaaaaag ttcattggcg gtgtactttc
     1201 agttggtgcg ggcctttttc taggtagaga aggaccttct atacagcttg gcgcatccat
     1261 tggacaagga gctggacaat tgtttcggag tccatcttca gaagaaaaga ttttaatttc
     1321 tagcggtgca agtagcggac ttgcagcagc attcaatgct cctatcgcag gattactctt
     1381 tgttttagaa gaaatccatc actccttttc tccacttgtt tggctgacat catttgcttc
     1441 agcaatcact gctaacttcg tctctcttta ctttttcggc ttgagacctg ttttatatct
     1501 aggtgacctt ccttctttgc cgttgaaata ttatggttcc ttagtgatct taggagtact
     1561 tctaggcatc ttaggtttta tttatcagaa ggtcttgctg gctttgccaa aattttacaa
     1621 ccgattgcct ttaccagctt attttcacgg gttcgttcct ttcttgctga ttttgccaat
     1681 tggttattta tggcctagta ctctaggcgg aggcaatcaa atcgttttag cttttggaca
     1741 tgcttccttg ccaatcatta tgattatcgg tttgtttgtt cttcgattcg ttttttccat
     1801 gatttcttac ggtggtggtc ttccaggggg gattttcctt ccgatattga cattgggggc
     1861 tttaatcggt agcttttatg gaaacattat ggttgatttc gggatggatc caatctacat
     1921 caaggatttt gtcgttattg ccatggctgg ttatttcaca gcgattggaa aagcaccgct
     1981 gacagcaatt attttagtga cagaaatggt tggctctctt aatcatctga tgcctttagg
     2041 attggctgct cttgttgctt atatcgtcaa tgaccttctc ggcggtaatc caatttacga
     2101 atcattattg gaacgtctac ttggaaatac tcagaccgcc tcactaaaag gtgcaaggac
     2161 aacttttgat tttccagtaa cggcagagag ttctttagac ggattaatgg tacgagactt
     2221 caattggcct aaaaatatgc tgctaatctc tatacgcaga ggaaatcagg aaatcttgac
     2281 tcacggtgat acgatcatga atgtaggaga tattttggtt atcttgactg atgaagcaaa
     2341 cttgccaaga atcaagcagg aaatcgaggc taaatcttct atcacaaatt tgaagcaatc
     2401 attttaacga acatggaaaa actattttac aacaactatt catctacact acttgattct
     2461 atatgattct ataaaaaaag aagagactgg gaaaaaccag cctcttcttt tttatttcta
     2521 tgcttctgct gctgcaggag cttttctcaa taaaccataa atcacaccag atacgactgc
     2581 tccgatagca ataaacacca aatacatgac cgggtgactc agtaagaata caacgaaaat
     2641 cccgccatga ggagccagta atttgatccc gaaagctccg actaatccgc cagttaaagc
     2701 agaaccaata ataaaacttg gaatggcacg aatcgggtca gctgctgcaa atggaatcga
     2761 tccttccgtg acaaacgata gtcccatcac gatgttcgtc aaaccagcat cttgatctgt
     2821 tttgctgaat ttattcttaa acaaacgtgt agcaacgaaa attgccaatg gaggtaccat
     2881 tcctccagcc ataacagaag ccatcacgac acttccgcca gttgcaacag tcgtagctaa
     2941 agttgctgta ccaaacacat aagcggcttt gttgattgga ccgcctaaat ctgccgccat
     3001 cattccagct aataatgcac caagtaaagc agcattcgaa ccactcatac ttgtcaagaa
     3061 accgttcaat gcatcattga ttgctttcat agggacattg accagcaaca tcaagaaacc
     3121 agtaatcagt aagccaaaca ctggataaaa gaggatcgtt ttaattccat ccaatgattt
     3181 tggtaagttc ttgaataatt tacgtaaaaa tacgatgaca taaccagcta agaatccacc
     3241 aatcagtgca cctaagaatc ctgcaccgcc tgtattagct aatgcaccag cagcaaatcc
     3301 aacgatcaaa ccagggcggt caccgatact tgaagcaata aatcctgcta ataccggcag
     3361 catgaaacca aatgcagctt gtccgatttg gttgaaccag ctagcagctt gattgtaatt
     3421 ccctagattt gataattgat catgtggcac gccaataaat tggtcgatca tgaaagaaag
     3481 cgcaatagcg attcctccac caataacgaa aggtaacatg tgtgatacgc cgttcatcaa
     3541 atctttgtaa attctttgtc cgatagtacc atccgcattt ccttcttctg aactgctttc
     3601 ttttccatca ccatggaata ctggtgcagt accactaagg gctagattga tcagctcttc
     3661 tggtttacgg ataccatcac tgacaggacg attgatcagt tcttttccgt caaagcgatt
     3721 catctctact tttttgtctg ctgcaacgat tacaccttct gcacgggcga tgtcttcagc
     3781 agtcaatcgg tttttgattc cttctgaacc gttcgtttcg actttgatct ccacaccca
//
LOCUS       DAYLZO010000186         3805 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.192, whole genome shotgun sequence.
ACCESSION   DAYLZO010000186 DAYLZO010000000
VERSION     DAYLZO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.192"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..276
                     /locus_tag="ACGVJG_002924"
     CDS             <1..276
                     /locus_tag="ACGVJG_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558816.1"
                     /translation="KRARTLRQQLKTYAEEKEQELEIIQTNSRQQLKQLVEEKEQFEK
                     QELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIERVKETYGRH"
     gene            263..2266
                     /locus_tag="ACGVJG_002925"
     CDS             263..2266
                     /locus_tag="ACGVJG_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFJ6558817.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            2285..2755
                     /locus_tag="ACGVJG_002926"
     CDS             2285..2755
                     /locus_tag="ACGVJG_002926"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFJ6558818.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            2776..3360
                     /locus_tag="ACGVJG_002927"
     CDS             2776..3360
                     /locus_tag="ACGVJG_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFJ6558819.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            3375..>3805
                     /locus_tag="ACGVJG_002928"
     CDS             3375..>3805
                     /locus_tag="ACGVJG_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFJ6558820.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREV"
BASE COUNT         1299 a          649 c          830 g         1027 t
ORIGIN      
        1 aagcgtgctc gaaccctacg gcagcagttg aagacctatg cagaagaaaa agagcaagaa
       61 ttggagataa tccaaacgaa cagtcggcaa cagctgaaac agttggtaga agaaaaagaa
      121 caattcgaaa agcaagaatt agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag
      181 gtgcaagcaa ctttgaaaga ccaatatcaa aagcatcaga gtcaagcaat cggtgcaatc
      241 attgaaaggg tgaaagaaac ttatggccgt cactaagatg gaaaaagtga ccctcatttc
      301 agataaaaga aatcaggaga ggattttaca agcagtacaa ggaatccaaa atgttgaaat
      361 ccgggatctg tatcaaaata gaacaaacaa ccagtgggta gaaaaatatt ttccagatac
      421 cacgattatt gataaagaag caaaattaag cgctttaggc aatcggctga aagacatcca
      481 agaagctatc caattcatac aacaccacgg cgatagccgt caaaagaaac tccatttgaa
      541 acatcaagaa ctttccttac aagacttgga agcaaattat tccgaagaaa atttcatgaa
      601 taaattagaa gaagtccttg atttaaaaga aaagtgggaa cagctaactg aaaaacgtga
      661 tcgtttagaa gaagaggaag aatggcttct tcattggcaa cagctggata ttgctccaag
      721 tatgtatgca agtaagacga ccatttttga gatggggact gtgagtgcag caaattttga
      781 aagtttcaag gaagaactaa gtaagcttga tgacgtttat atggaagaaa tagattatac
      841 gccaaaacaa gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg ctatagaaga
      901 aattgctggg aaatacgggt tcaataaaga aacgtatcct tatgaacaaa atcctaaaga
      961 aaaattgcaa gaaaccaaac agcaattatc cattgttcac gagcagcaaa aaaagataac
     1021 gacagcgtta ggtcagtgca gcggctatat ccaagaattt gaatgggtag aagaagtcac
     1081 acttgccgtt gcagaacggg aaaaagtaaa agaacggttt atccatgcca cttatctgat
     1141 cgttttgcaa ggttgggtag atgcagaaga aaaacagaat ttgatgcatg tgttaaacga
     1201 aactgtatca gaagaagata tatacgttag tttcgaaaca ccaaactcag aagagattca
     1261 gcaggaagtc cctactaaat tgaaaaacca tccgcttatc gaaccttttg aattgctgac
     1321 tgaaatgtac agtctgccaa aatatgaaga agtagatcca acgccttgga tgacaccatt
     1381 ttatcttgtc ttcttcggga tgatggtcgc agatgttggt tacggattgc tgatgctcat
     1441 tgggacaata ctagctcaaa aactatttgt tttaccccgc gggatgaaac gtttcgtaaa
     1501 attctttgag attctctcgg taccatcgat catctgggga ttgatctaca gttcattttt
     1561 cggacaggca ttaccaaaag aactatttgg tatccgcttg cctttcccaa tcctatctac
     1621 gacggatgac gtcaatacga ttttgatttt atccgtgatt ttcggtctga tacagatctt
     1681 ggttggatta ttcgtggcag caaaggaaaa tatcaggagg aaagattact tgagtgctgt
     1741 cagtgatggt tttgcctggc aagggatcct gattgggatc gttattgcat tgattggtgt
     1801 catgttgata aaaaatacag catttgttta tcttggcgcg gggctatcga tcattgctgc
     1861 tttatgtatc ttaatcgtgc cgattatcca atcgccgtct aaagtcaagg gtgctgccaa
     1921 aggagcgtat aatctttacg gattgactgg ttatatcggt gatttagtca gttatacacg
     1981 tttgatggca ctaggtattt caggcggaag tattggcgcg gcattcaata tgctagttgc
     2041 tttcatgccg ccagccgctc gattcagtgt agggatcctt ttgatcatcg ctttacacgc
     2101 actgaatatg tttttgacac tgttaagtgc ctatgtccat ggtgcacgat tgcagtatgt
     2161 cgaatttttt ggaaaatttt atacaggagg cggacgtgcc tttgacccgc tgaaaacggc
     2221 ggaaaaatac gtcaacatca atcataagaa aaaaaacaat aaataattgg aggattttta
     2281 tacaatgatg gattacttaa ttagtcaaaa tggtggaatg gtatttgcag tattagcgat
     2341 ggcaacagcg acgatttttt cagggattgg ttcagctaaa ggtgttggaa tgacaggtga
     2401 ggcagcagca gcgttaacaa caagtcaacc cgaaaaattc ggacaggctt tgattctgca
     2461 attgcttcca ggtacgcaag ggttgtatgg attcgttatc gcctttttga tttttatcaa
     2521 cttaggaaat gatatgtcag ttgtacaagg attgaacttt ctaggcgctt ctctgccgat
     2581 tgcttttaca ggtttgtttt caggtatcgc ccaagggaaa gttgcagcag caggtatcca
     2641 aattttagcg aaaaaacctg aacatgctac aaaaggaatc atttttgctg cgatggttga
     2701 aacatatgcg attttaggtt tcgttatttc cttcctatta gtattgaatg cctaagcata
     2761 aaggaggaaa agcagatgaa cgccattgaa aaaatcatct ctcagatgaa tgaagcagca
     2821 gagcaagaaa gagcggcgtt agagcaagaa gagcgaatga agatcgatca aaactttgaa
     2881 caaaaacgta cgcaaatcga aacagaacat caaaaacaaa aagaaaaaca aatcgaatta
     2941 cttgaaaaaa aataccgtca gcttaggaat cgccaacaag tagaagtacg acaagaaaat
     3001 ttgaatgcga agcaggaatt tttacgacga ttgtttgcag atgcaatagc tgaaatggag
     3061 aattgggatg aatctgaaca aatccagttt atcaaaaatt cattgtattc attacctttg
     3121 acaggcaaag taacattcat tgctggagaa aaatcagcag cttatctttc tcagacattg
     3181 ctggatgaat ggaacaatga acttcccttt atgatggttt taagtgatga aactgttgct
     3241 gatcaagcgg gttttctgat aaacgatcaa ggagttcagt ataattttct tttcagcagc
     3301 cttgtacaag atatccaagg aacgatgagt tttgagattg ccaatcaatt attcgaataa
     3361 ggagggatca gtgtatggaa taccacgaac taaatccctt gattcgcgga agagagcttg
     3421 aacttttgtc caatgagaca tttgaacgga tgattcaaac aaattctgtc gaagcacttg
     3481 gtgaaatatt gaaatccacg atttacagtc catatattta tgacggatat gaatatgact
     3541 tcgaacagaa tcttttaaag gagcagagcc gtctatttcg ctggttaaag gaatcagcac
     3601 cggagcctga aatcgtttgg atctatacga tgcgctatac tttccataat ctgaaggttt
     3661 tgacaaaagc cgagatgaca aatcaaaatc tggaccattt gtatatcgac gatggatttt
     3721 attcattaga aaccttgaag gatgccattc atacgcaagt ttcaaatgaa ctccctgaaa
     3781 gcattatgga cagtatacga gaagt
//
LOCUS       DAYLZO010000187         3792 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.193, whole genome shotgun sequence.
ACCESSION   DAYLZO010000187 DAYLZO010000000
VERSION     DAYLZO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.193"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..2671)
                     /locus_tag="ACGVJG_002929"
     CDS             complement(<1..2671)
                     /locus_tag="ACGVJG_002929"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFJ6558821.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            3239..>3792
                     /locus_tag="ACGVJG_002930"
     CDS             3239..>3792
                     /locus_tag="ACGVJG_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFJ6558822.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVY"
BASE COUNT         1243 a          613 c          685 g         1251 t
ORIGIN      
        1 ttttttgtgt cgcccatgtt gcactgtact ggttacctgc aacaagatca tattgtatca
       61 gacgataatc ttctagaatt tctttttgtt tctcagttaa atttttttct tggataattt
      121 gactatcctc tgtaacaaag acctgttttc cattaaaacg attagacaag ctttctgtag
      181 gatcaattgt taatgcaggt agatgattag atacttctgt caacaacgca tagtaaggag
      241 ttactttgat attcgcatgc tcaagagcca tagcaccaaa gttattagga gcaacaactt
      301 ctttatttaa cgtagctgct tgttcacgac tatacttgtt gttataaatg aaataatctg
      361 tctggtgctg ttctaatcca tattttttcg aatcattccc actatagatt gcgggtaaat
      421 ggtcaccata aaaaacaaag gtaatcggtt tatcgatttt gtcgatttct tggatgaatt
      481 tttttactgc tgtatcggta tagtgaaggc cttgcatata agtttgtaat tcttttcttc
      541 tattaggaat gacagcagtt ccttcagcag tgtagttcaa ttcactatag taatcatcat
      601 aaggcatatg gttttgcata gtggataatt ggatgaattg tgacgtttct gtattgcttt
      661 gtaaagcttt caaggtttct ttgtatgctg aatcatccga tatgcgtggg ctttgttcta
      721 ttttatccgt ataagttaat ttatccggac tgtctaaata atagaacctg tcaaaaccaa
      781 atttatcaaa cacttgtttt ctgcgataaa gagcagctgt aaaaggatgg atggctattt
      841 tttcatcgaa tagatcggta atatttggac ttattttctg acgctccact agctgggtat
      901 aaggtgtaac taacgaagca gacaggttac tgatactcaa gcctgtcagt ccttcccatt
      961 ccatgttagc tgttccgcca ccataaccga cggataacat caatcctcct gttccttctg
     1021 ttataatgct tttgatgtaa ggaatagggt ctgtaggtac tgtcagatta gggacacgtg
     1081 caggatcact gaagctttca cttaaattga gaacgatcgt ctgattgttt aaccagtcac
     1141 ttcttgtttg gttgatttca tctgcctttg tgttgtactt tttcattatt ccattaattt
     1201 tttctttcga atagccagca ggttcttcca tgattttaat gtcgatattg tttaggaatt
     1261 gaatgactgg tccgttatct tttacggcgc cttgctggtt aaagaaagtc ttgtttactt
     1321 taaataaatt aaacagcaag aaagaaggcg aatttttgtg attaatgaaa aagacacccg
     1381 aaaaacatcc taataataca gcgatactga taaatcgacg tttccaatga atcttcaagg
     1441 aatactgttt ttgtagtcta cgttgtacaa taatactagt aatcaataat aggagaagga
     1501 tcaatccgcc cacgattaat aagactgggc taaccatcga taatagttct gacaatccgg
     1561 tcagtatttt taaatctgaa ggaaagacag gctcttcccg catttccatt ttgattactg
     1621 tcgaaatagt gagcagtaag ttaatgatca aggtagcagt caaggcgtac caaaaacgat
     1681 tggaaatcaa caatagtaag aagaaaaatc ccataataat gatgattgtc aggatcatgg
     1741 gtgcttgtcg ctgagtaaat atttttaagg caacctggat agtttctttc caaccttctt
     1801 ttggttctaa aacgaaaatc tgcacgaagg taaagcagta aaaaaatgct gcaactaaaa
     1861 aataactttt atgaacaaaa agccaatgaa ttcttttttt ccaacgctga gttaattgtt
     1921 caatcgaatc aaaagcgaag tattcgatga aacgatgaat gaaacggatt ttttgtatat
     1981 aaaataaaaa gataactagg agggacactg ctaataaata gcaaataaaa aactgcccga
     2041 tatttatcag ccatgccttc cctgagtttg gaaattcacg tttcataaat gtagatacgc
     2101 gataagaaac taaagggaaa ttacatataa cttgagtgac aatcaaaaat acagcaagaa
     2161 aatttcctct tccttttata cctaatttat cagatagcat ttctaaaaat aaaaacgaag
     2221 agaaagtata atacattgcg aagagagaat atggtttaga aaacctcagc gctgtatctg
     2281 tatttcctct gatgatgagt gaaatttgtg tcatcaaaat aatcaaaccg aataaaacaa
     2341 tcaagctgat aaatacttga ggaagcttat agcggacctt ttctttccag ttccattctt
     2401 tcaaaccata acccattaaa aataaataaa aaatccataa aatactctgt ccattctgta
     2461 atgcaaacgg gtctttatta aatagagtag agcagccaac taatccaatt gttaaaaata
     2521 atagtagata tttaattgtt gttttcgata atttatttaa aaatccaact gtataaggaa
     2581 tactacaaaa aagcaaagta caagaaacag cataagtaaa gtaattctga ctgataggtg
     2641 ttaggataag ccaataatca cgaatattca agctgccagc ttgtacgtaa aaaaaagata
     2701 ttgcaaacta gtgtggctat taggaatata aaggcaaaac gacttaaata atgtaaaact
     2761 tttaaacggg cgattttttg atattggtaa cccatgtaaa gtgggatcaa atttgcacca
     2821 ataacaacgg aagtttgtaa aaatgtatag atgtagaatc gaccagggtt tgttgcatat
     2881 gtaggaatag tgactgctga aatcaatata cgctgtaaaa ccatcattat acaagcgaca
     2941 aagattgtca ggaagccata actctccttc tttatttttt tcaagaataa acacctcaca
     3001 aaaaaattgg acaaaaacat aagaaaacgt ttcctgataa gttgcccatt aataacatcc
     3061 taacttagaa tatctcaaaa aacaacgtgt agacagaaaa agtacaaaat tagcaagaaa
     3121 tggttgtttg tattgttaga atttttagaa aattatacct ttagtttctt tgcataaaat
     3181 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     3241 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     3301 ccccacatgg acatttattt caatattggg aacgttgttt gtcagttgct tgaaggcaat
     3361 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     3421 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     3481 tgtagcagtt attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     3541 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     3601 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gtcttattct gggctgtatt
     3661 gttgggacta gcttttcgag cacgttcaga atcgacaaaa gagttggtgg atcagatatc
     3721 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     3781 attggtttat ca
//
LOCUS       DAYLZO010000188         3625 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.194, whole genome shotgun sequence.
ACCESSION   DAYLZO010000188 DAYLZO010000000
VERSION     DAYLZO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.194"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..567)
                     /locus_tag="ACGVJG_002931"
     CDS             complement(<1..567)
                     /locus_tag="ACGVJG_002931"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFJ6558823.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAH"
     gene            complement(585..1499)
                     /gene="pfkB"
                     /locus_tag="ACGVJG_002932"
     CDS             complement(585..1499)
                     /gene="pfkB"
                     /locus_tag="ACGVJG_002932"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFJ6558824.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(1496..2248)
                     /locus_tag="ACGVJG_002933"
     CDS             complement(1496..2248)
                     /locus_tag="ACGVJG_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFJ6558825.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(2407..2616)
                     /locus_tag="ACGVJG_002934"
     CDS             complement(2407..2616)
                     /locus_tag="ACGVJG_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558826.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(2627..>3625)
                     /locus_tag="ACGVJG_002935"
     CDS             complement(2627..>3625)
                     /locus_tag="ACGVJG_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558827.1"
                     /translation="IQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLP
                     KMKQQNGEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKV
                     KELENEVICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIY
                     TDHTIQSVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDE
                     NRSRMMCYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLS
                     ELPLKEIKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT          998 a          743 c          633 g         1251 t
ORIGIN      
        1 gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt ctggatgagc
       61 agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt tttgctcttc
      121 agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta attttcctac
      181 gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat gtgtgtcgtt
      241 tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt ctaaggcttc
      301 ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa ctgcactgtt
      361 tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg cttcgcgggc
      421 tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa acattttttg
      481 gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga tcatcgcttc
      541 tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc aatttttgtg
      601 atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc atcagaaaaa
      661 gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc tttcgtacgg
      721 ctgtaagccc caataaagcc ggcaatcatt gaatcccctg cacctactga atttttcaat
      781 gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa agcaccgtct
      841 ccagccattg agatcagcac attctgtgcc ccttcagcta gcaatttttt accatacgga
      901 atgatttctt ctacagattg gaaggttgta ttgtaaagct cagctaattc gtgattgttt
      961 ggtttgatca acaatggttt cttaacaagt gcatttttta ggtcatcacc tgttgtatca
     1021 atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata aaatcctttt
     1081 cgcaggctag ccggcgcgct tcctgacaat acgacgatat ctccttgtcc tactgttcct
     1141 aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcccaa accgttaatt
     1201 tcagtttcag tatctgattt tagtttgata ttgattcttg tatcttcttt tacggttgtg
     1261 aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc agtgaaccct
     1321 cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg ggaaacattg
     1381 atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc gcctagtttt
     1441 aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt gtaaatcatt
     1501 gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct aatggacaac
     1561 gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac aactggttga
     1621 atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct aaacgtttaa
     1681 cagctgcctc ttctgggtcc ggagtcgtga atccttgttc aagatgggca ccgttcattc
     1741 ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta gatcctaaaa
     1801 cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc tccaaatcaa
     1861 ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt ccttgcagaa
     1921 aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct tcgtcattaa
     1981 tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg agtgattttt
     2041 cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca tgaactcttt
     2101 ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat tcagaagcat
     2161 taaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc aagtctagga
     2221 ttttttgtct acgttcctct gtaagcattt tcacgatcac ctcaatacaa ataatagcat
     2281 attttttttc tactcgcaat ataaatcagt caaaaacgtt cataaaaatt ataaatcagt
     2341 caaaaaaaaa taaaaaacca gtcaaaaaga ctggtttttt atttttttgt ctatcatcaa
     2401 gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag aagcaggttt
     2461 tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga ccaaaaaacc
     2521 gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata ataatgatgc
     2581 cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat attttttgta
     2641 aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga tgctgctcac
     2701 ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg aaggtaacat
     2761 cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct gaaggaacat
     2821 agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct acttctagat
     2881 attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca gtcggatcga
     2941 taactttata gcacatcatg cgcgagcgat tctcgtcaac ccaaggaaaa tacgcctgag
     3001 catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttgaata tcataacttt
     3061 ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca ttctgatact
     3121 ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt ttcaataaac
     3181 cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa taggattgaa
     3241 acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg atacatatga
     3301 cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa atcggtttag
     3361 ggatgatttg atgataatag cgactataat acatttccca acacgaagtg aaacataaac
     3421 cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact tccccgtttt
     3481 gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat agccgttgag
     3541 agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag gcgaacaccc
     3601 catgagcaat catttttttc tgaat
//
LOCUS       DAYLZO010000189         3619 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.195, whole genome shotgun sequence.
ACCESSION   DAYLZO010000189 DAYLZO010000000
VERSION     DAYLZO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.195"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..690)
                     /gene="pstB"
                     /locus_tag="ACGVJG_002936"
     CDS             complement(<1..690)
                     /gene="pstB"
                     /locus_tag="ACGVJG_002936"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFJ6558828.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARIS"
     gene            complement(705..1589)
                     /gene="pstA"
                     /locus_tag="ACGVJG_002937"
     CDS             complement(705..1589)
                     /gene="pstA"
                     /locus_tag="ACGVJG_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFJ6558829.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(1589..2509)
                     /gene="pstC"
                     /locus_tag="ACGVJG_002938"
     CDS             complement(1589..2509)
                     /gene="pstC"
                     /locus_tag="ACGVJG_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFJ6558830.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(2624..3481)
                     /locus_tag="ACGVJG_002939"
     CDS             complement(2624..3481)
                     /locus_tag="ACGVJG_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFJ6558831.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
BASE COUNT         1079 a          820 c          641 g         1079 t
ORIGIN      
        1 gctgatacga gctgcttgtt gcatgttatg cgtcacgatg atgatcgtat agtcttcttt
       61 caagtttaca agtgtttctt caaccgttcc tgtcgaaata gggtccaaag cacttgccgg
      121 ctcgtctagc agcaagatat caggtttcat cgcgatcgca cgagcaatac ataaacgttg
      181 ctgttgcccg ccagataaag ccaaggcact tttgtccaag ctgtctttta cttggtccca
      241 caaggcagct tgtttcaagc ttgtttcaac gatttcatct aatttctttt tgtttttttc
      301 gccgtgctgt ttcaaagcaa atgtaatatt gtcataaatc gacttgctga acggattagg
      361 acgttgaaaa accatgccaa tccgtttgcg catctcataa acatccactt gaggcgcatt
      421 aatatctacg ccttgataca tgattttccc agtgattttc gtattagcga tcccatcatt
      481 cattcgattc aacgaacgaa gatacgtaga ttttccgcag ccggaaggac caatcagtgc
      541 agtgatctta tttttttcaa attccagatc cactcctttg attgcttcat tgtcgccata
      601 ccagacatgt agatcatccg tatatagtgc tacttctttc tcttttggca tggtgatgac
      661 atttgtttca ttccagttat attttttcat tttgcttctt ctccttaggc tgaagtcatt
      721 tttcggtata atcgtttgcc aatcgcacga gcgccgaagt tgaacagtaa aacagcaatg
      781 atcaacacgg cagaagcgcc agccgaaaca gaaacaccat caggcatcgt gccttctgta
      841 ttgatcttcc aaatatggac agctaatgtt tcagcttgac ggaaaatgct gatcgggctt
      901 gaaacactta atggattcca gttcgtaaaa tctaacgcag gtgcactttg tccagccgta
      961 tagatcaatg cagctgcttc accaaaaatt cttccagaac ttaggatcac accagtcaaa
     1021 atccctggtg tcgcttctgg aaccaccact tttatcactg tttcccagcg tgacaaacct
     1081 aaggccaatc ctgcttctcg ttgtgtgtaa tggaccgctt gtaaagactc ctcaactgtt
     1141 ctagtcaaaa gaggcaagtt gaatacagtc aaagccaatg caccagaaat aatcgagaaa
     1201 ccatactgga attgaataac gaaaaccaag aaaccgaata aaccgacaac gacagaaggc
     1261 aacgagctca agatttcaat cgacgtgcga atcacatctg ttagccagtt ttttccagca
     1321 tattctgaaa gatagatacc cgcacctaaa gaaataggaa aactgatgat catcgtgatc
     1381 aaaagcaaat aaatcgagtt aaatagctgg atcccgattc caccgccttc ttggtaggct
     1441 ttggaaggtt cggtcaagaa ggaccatgaa atatgcggta ttccccgaac taagatataa
     1501 agtagtaaag ctgctaaaat caatacgatg atccccgaaa ctatatataa aacacctgta
     1561 gcaattttat ctgagcgttt tgcgttcatt atttcagctc tcctttcttc ccaatcatac
     1621 ggatcacgat attaaagaat aatgacatca atagcaagat cagcgctaat gaccataaca
     1681 cattattttc aactgttccc atgattgtat ttccgattcc cattgttaag atggacgtca
     1741 atgtagaagc aggagtagta agtcctgttg gcatcaatgc tgcattaccg atgaccattt
     1801 ggatagctaa ggcttcacca aacgcacgag ccataccaaa aacgactgct gttaaaatcc
     1861 cgggcacggc tgctcgtagc acgactttgt agattgtttg ccatcttgtg gcaccaagcg
     1921 ctaatgaagc ttcccgatag tgccgtggca ctgcctttaa tgcatcaaca gtcatcgttg
     1981 tgacagtcgg caagatcata acgaacaaca caaatgtacc agctaagata ccaaaccctg
     2041 tcccgccaaa aatacttctg acaaaaggca cgataactga taaaccgata aaaccataaa
     2101 cgacagaagg aatacctaca agcagctcga tgaccggctg taagatcttc gttccgtttt
     2161 taggagagat ctccgtcatg aatactgctg caccaatggc aaatggcgtg gcaacgatag
     2221 cagataaaaa ggtcacaata aacgaacctg tgatcatagg caatgcccct accattggtt
     2281 tcccatttgc tccgatttgg cttggattcc aatctgtacc aaacagaaaa tcaaatacat
     2341 tgatcttatc agtaaaaaag gtggccaatc ctttactagc tacaaaataa aaaatagata
     2401 atacaaccaa cacgatcagc gcgatacata aaaaactgat gaattttcca cgctgttcca
     2461 tttttgaacg ttttgatttt gttaataata cttttttcac atcttccaca gatgactccc
     2521 ccttaatcag gtttaacata ctcactcagt gtacaaattg aacatcaatt tggttttaat
     2581 aacatgtaaa agtttagcta agtttgtaaa ttttgtgtaa agattactct ttaacgatat
     2641 ttccttgcca atcacgttcg actttcatat gagaaacagg gatataacct agttctccaa
     2701 cgatgttatc ttgtacttca tcagataaga tatagtctaa aaattctttt gtcagttttt
     2761 ttggatgatc tcttgtgtac atatgctcat atgcccaaat cgtccatttg ttcgttgtca
     2821 cattttcatc tgtcggttct acaccgttaa cattcaacgt atctacatca tcggaaacat
     2881 aagaaaaagc aacatagctg attgcaccgg gtgtgtcggc aacgatctgg cgcaccattc
     2941 cgcttgaatc ttgttcttgt gcctgaacag ctgtttctcc gtccaatacc catttttcaa
     3001 acgtgctacg ggttccgctt cctgaagcgc ggttcaatat aactactggc tgatcttttc
     3061 caccgacttc actccagttt ttgatctcgc ctgtaaatag ttttttcagg ttttccatac
     3121 tgatgtcttt tacgccaata tccttgttca caataggagt gatcccaaca actgctactc
     3181 gatgatcgac taatgctgag gcatcgatcc ccgatttttc ttccgcaaac aaatcagagt
     3241 ttccgatgtc gactgctcct gactgtactt gagacaaacc agttccgctt ccgccccctt
     3301 gtacattgat gaattttcca ggattttctg tctggtaagt ttcagcaact gtttctacta
     3361 aaggctgtaa agcagaagag ccaacagccg taatggattc tcctcgatca atccactttg
     3421 aacagcctga taaaagaatg gctaatccta ttattggtaa gaataattta agttttttca
     3481 ttagaaatct cctttaatcc taaagtatgt ttattttatc attttcatta cttgatgaaa
     3541 acgaatcaaa atcattaaat aacaaaataa attctcataa ttgtaaaaaa aactatctca
     3601 ttaaaagttt ctatgctat
//
LOCUS       DAYLZO010000190         3505 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.196, whole genome shotgun sequence.
ACCESSION   DAYLZO010000190 DAYLZO010000000
VERSION     DAYLZO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.196"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1548
                     /locus_tag="ACGVJG_002940"
     CDS             <1..1548
                     /locus_tag="ACGVJG_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFJ6558832.1"
                     /translation="ASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEK
                     GDTLADGPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYE
                     SEARDTKLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTEL
                     SAEERLLHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVY
                     IVQKRKIHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQ
                     VLELHLGMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDG
                     RVSVGVMYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAY
                     GAAYTLQEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRV
                     LDIEETEIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFE
                     TAEDNLD"
     gene            1627..>3505
                     /gene="rpoC"
                     /locus_tag="ACGVJG_002941"
     CDS             1627..>3505
                     /gene="rpoC"
                     /locus_tag="ACGVJG_002941"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFJ6558833.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AE"
BASE COUNT         1115 a          688 c          822 g          880 t
ORIGIN      
        1 gcttctgaaa tccgcgtacg ccgcgacaac ggtgcattag ataaatacga tgtaacaaaa
       61 ttccgtcgtt caaactccgg aacaagctac aaccaacgtc caatcgttca tttgggtgag
      121 aaagtcgaaa aaggggatac tctggcagac ggaccatcta tggaacaagg cgaaatggct
      181 ctaggccaaa acgttttagt tggtttcatg acttgggaag ggtacaacta tgaagatgcg
      241 atcatcatga gccgtcgttt ggtaaaagat gacgtataca cttctatcca tattgaagaa
      301 tatgaatcag aagcccgcga tacaaaatta ggacctgaag aaatcacacg cgaaatccct
      361 aatgtcgggg aagacgcatt aaaagattta gacgaaatgg gtatcatccg tattggtgca
      421 gaagtccaag atggcgattt gcttgtaggg aaagtcacgc caaaaggggt aacagaatta
      481 tctgcagaag aacgtctatt gcatgcgatc tttggcgaaa aagctcgtga agtccgcgat
      541 acatcattac gtgtaccaca tggcggcggc ggtatcgttc acgatgtgaa aatctttact
      601 cgtgaagctg gagatgaatt gtcaccaggc gtaaacatgt tagttcgtgt ttatatcgtt
      661 caaaaacgta aaatccatga aggggataaa atggccggtc gtcacggaaa taaaggggtt
      721 gtatcccgta ttatgccgga agaagatatg ccattcctac ctgatggaac accaatcgat
      781 atcatgctga atcctttagg ggtgccttca cgtatgaaca tcgggcaagt attggaatta
      841 cacttgggta tggctgcacg tcagttaggt atccacgtag caacgccagt atttgatggt
      901 gcaagtgatg aagacgtatg ggaaacagtt cgtgaagccg gtatggccag cgatgcgaaa
      961 actgttctgt acgacggacg tacaggtgaa ccatttgatg gacgtgtgtc tgtcggtgtg
     1021 atgtacatga tcaaacttgc tcacatggtc gatgacaaat tgcatgcacg ttcaatcgga
     1081 ccttactcat tggttacaca acaaccattg ggaggtaaag ctcaatttgg tggacaacgt
     1141 tttggtgaaa tggaagtatg ggcactggaa gcttacggtg cggcttatac tttacaagaa
     1201 atcttgacat acaaatcaga tgacgtagtc ggacgtgtga aaacatacga agcaatcgtc
     1261 aagggtgaac caattccaaa accaggtgta ccggaatcat tccgcgtatt ggtgaaagaa
     1321 ttgcaatcac taggtctgga catgcgtgtc ttggatatcg aagaaacaga gatcgaactt
     1381 cgcgacatgg acgatgaaga cgacgatttg atcactgttg atgctttgac aaaatttgcc
     1441 gaacagcaaa ctgccaaaga attagaaaag aaagctgctg aacaagtgga agatgaaaaa
     1501 gacgatgtca tccaaaactt tgaaaccgca gaagataatt tagactaatc cggtctatga
     1561 gctgcaaggc ggtcgaagat cagccgtctt gcggtttgcc attaaatgaa atggagggaa
     1621 ccccttttga tcgatgtaaa taaattcgaa agtatgcaaa taggtttggc ttctcccgaa
     1681 aaaatcagaa gctggtctta cggtgaagta aaaaaacctg agacgattaa ctatcgtacc
     1741 ttaaaacctg aacgcgaagg gttgttctgt gagcggattt ttggtcctac aaaagactgg
     1801 gaatgtgcct gcggaaaata caaacgtatc cgttataaag gaatcgtttg tgatcgatgc
     1861 ggagtggaag ttactcgttc gaaagttcgc cgtgaacgca tgggacatat tgaattagca
     1921 gctcctgttt cacatatctg gtactttaaa gggattcctt cacgtatggg tcttgtatta
     1981 gacatgagcc cacgcgcttt ggaagaaatc atttattttg cttcatatgt agtcatcgaa
     2041 ccaggaaata cgaatttaga gaaaaaacaa ttgttgacag aacgcgaata tcgcgaaaaa
     2101 cgtgaacaat atggacaaga attccaagct gctatgggtg cagaagcgat taaacaatta
     2161 cttgataatg ttgatctaga tgcagaagca gccgaattga aagaagaatt gaaatctgca
     2221 caaggacaaa aacgtacacg tgcaatccgc cgtttggata ttttagaagc attccgcgct
     2281 tcaggaaatc aaccaagctg gatggttatg gacgttatcc ctgttattcc accagattta
     2341 cgcccaatgg ttcaattaga aggtggacgt tttgcaacaa gtgacttgaa tgatctttac
     2401 cgtcgtgtaa tcaaccgtaa taaccgtttg aaacgtttgt tagacttgaa tgcacctgga
     2461 atcatcgttc aaaatgaaaa acgtatgctg caagaagcag tagatgcatt gatcgataac
     2521 ggtcgtcgcg gacgtccagt tactggacca ggtaaccgcc cattgaaatc tctttctcat
     2581 atgttgaaag ggaaacaagg tcgtttccgt caaaacttgc taggtaaacg tgtcgactac
     2641 tcaggtcgtt ccgttatcgt tgttggacct ttcttgaaga tgtaccaatg tggacttcca
     2701 aaagaaatgg ccattgaatt attcaaacca ttcgtgatgc gtgagcttgt tcaacgcgaa
     2761 ctagcttcaa acatcaaaaa tgcgaaacgt aaaatcgaac gtcaagaaga cgaagtatgg
     2821 gatgttttag aagacgtaat caaggaacac ccagtattgc tgaaccgggc acctacgctt
     2881 caccgacttg gtatccaagc attcgaacca gttttagtac aaggacgcgc aatccgtttg
     2941 catccacttg tatgtgaagc ttataatgcc gatttcgatg gagaccaaat ggctgtccac
     3001 gttccattga atgaagaagc acaagcagaa gcacgtatgt tgatgttagc tgctcaaaac
     3061 atcttgaacc ctaaagatgg taaaccagtt gttactcctt ctcaagatat ggttctaggt
     3121 aactactact tgacaatgga agaagaagga cgcgaaggcg aaggaatggt cttcagagat
     3181 caaaacgaag ccgttatcgc atggcgcaac ggatatgttc atttacattc acgtatcggt
     3241 gtgaacccta actcattagg tgaaaaacca tttactgaat ggcaaaaaga acgtacgatg
     3301 atcacaacag ttggtaagat catcttcaac gagatcatgc ctcctgaatt cccttatctg
     3361 aatgaaccaa cagacttcaa cttgaccgtc caaacaccgg acaaatactt tgttgaagct
     3421 ggtactgata ttcctgcaca tatcaaagag caagaattag tattaccgtt caagaagaaa
     3481 aacttaggaa acatcatcgc agaag
//
LOCUS       DAYLZO010000191         3412 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.197, whole genome shotgun sequence.
ACCESSION   DAYLZO010000191 DAYLZO010000000
VERSION     DAYLZO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.197"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(173..1177)
                     /locus_tag="ACGVJG_002942"
     CDS             complement(173..1177)
                     /locus_tag="ACGVJG_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558834.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(1636..1893)
                     /locus_tag="ACGVJG_002943"
     CDS             complement(1636..1893)
                     /locus_tag="ACGVJG_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFJ6558835.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1886..2155)
                     /locus_tag="ACGVJG_002944"
     CDS             complement(1886..2155)
                     /locus_tag="ACGVJG_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFJ6558836.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(2286..2465)
                     /locus_tag="ACGVJG_002945"
     CDS             complement(2286..2465)
                     /locus_tag="ACGVJG_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558837.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            2563..2799
                     /locus_tag="ACGVJG_002946"
     CDS             2563..2799
                     /locus_tag="ACGVJG_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558838.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            complement(2846..>3412)
                     /locus_tag="ACGVJG_002947"
     CDS             complement(2846..>3412)
                     /locus_tag="ACGVJG_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558839.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         1038 a          637 c          439 g         1298 t
ORIGIN      
        1 acaaatagtt gatcatgctg cttttattat cttctcatct ttaatttcct acatataatt
       61 actatctata aaattaagtc ttactcagaa cagataaagc ataaaattcc aacaaaccat
      121 tttttactta actaaataaa gagtctgtta atataatctt cacttattta cgttaatccc
      181 ctactgtatt tgttggttcc ttatctatat tttctacaaa atccttatta tcttgtgatt
      241 tagcttcgta taattctttg tatgtcgaca cattggttat actaattcct atagctagtt
      301 cttgagactc tacttttctc tctagctcct ttgacaaagt gatttgagat ttatttggtt
      361 ccgttggagt caattctata gattcttcat aaaaatctct atacataaag ctatcattaa
      421 ttttataaaa aaaattaaat gtttttagca ctgccatata acattcaaaa cattcttcta
      481 tagttggttt acttccttga ggagaatgaa ccaatttatt acgtttttct atgaaatttt
      541 taatttcctt tcttatttct ttcggaaact caatattaaa gtacccttta aacatgctcc
      601 ctaataattt gtctacaggg ggagattgta aattctcaaa tattagagtt aaatcaggtt
      661 tctcttgttg ataaaattcc tttaccccta tttcacaggc tgtaattatc gaaataaacc
      721 tatctctagt atttgatata tttttacttc ttttaaggag tataagagaa aaggggatac
      781 tttcgtgtcc atgcatgtaa ttaactaatt gttgagcttc agaagttata tctctaatta
      841 tatagttaac ggaattagga agtgtcttta actctaatga aatttctatc caattactat
      901 cttctaatct gtatatgctc gaagtataat ttgattcgtc agcaagcatc gctacatccc
      961 aaacactgat cataaaatct tttaaacgtt caatcatttc agcaatattg ctaaaatcag
     1021 ctaatttttc tttagaataa aaaaaaacat ttatatcaga atcaataaat tctatttttt
     1081 ttattactat atcgtcatca ttatcttgac catttatgtt tattgttccc tgagtgggga
     1141 gtacttcttt caaattcaag ttaaatttat attccattac ttctcctcca atatataaaa
     1201 attacttaac tgtatccaaa tttaatatgt aaataaatca ttaattttta gctaaattct
     1261 atgagaagtt tatttttgtt gtttttgcta attaactaaa actagtacaa aaaatttaaa
     1321 atagattatt aaacctattg cttattctat tcagtcatat atataagcgt catcctttag
     1381 taactaacat cccacactac aatagtatta taacatatct attcaacttc aaattgttga
     1441 aatttaagcc atttatattt acttttcctc aactttagaa atgattttac taaccttata
     1501 tccgtaatac gcgtaacaaa cacattcatt aataacaatc aacactaagc tatattagct
     1561 ttttaaatga ctagggtaaa taaaataagg atcaaaaact tttaagtttc tgaccctttc
     1621 cttacttccg attggttaat agtgatcttt tgcagaataa ataaatatcg tttcattttc
     1681 aactctatat atcagtctat gttcatctgt aattcttctg gaccattttc cagataaatc
     1741 atgctttaat ggctcaggtt ttcctaatcc agcaaagggg gaacgatcga tatcttttat
     1801 taacttgtta atctttttta tattgctttt gtttccttgc tcatgccaat aaagataatc
     1861 atcccaagca tcatcagacc aagccttaat catcagattc aacctcgatt aagtcatgtg
     1921 ttttaaatgc acctttggag aattgttcat ctcctcgacg aattttttcc atgacgtaat
     1981 tattagaaag tgttctcaac gtttcttgca tagaatcata atctcttttt gataatacaa
     2041 caactgtatc ttctacatct ttacttgtta caataagtgt ttcagcatcc tcattaactt
     2101 gtttcatata actacgtaaa ttttggcgga aatttgaata agctactgct tccattcctt
     2161 tcacccctta tttatttcta taaaacaatt gtacattata ttgtacaatt aagcaaatga
     2221 ttaatattct aacttcttat aattaaaagt gctatcactt ttagttttta ggataaaaag
     2281 tgacgtcact ttttgagtgc tggtacctca aatcctgctt tttcttgggc aatcaataaa
     2341 tctaacgcaa tacacataat cctggattca cttttgccgt acttctgctt tagttgctgc
     2401 aattcttttt gtctcttttc atccaacgta acgggtaacc gtttatttcc tattgcaatt
     2461 ggcattagta ctctctcctt tcatcgaaat aaccctatga aaaatatggc tgcaatcatc
     2521 ttgaatagta gactcacaag ggctaataca aagcgccaaa agatcaaaca aaataccagt
     2581 attgccaaaa atactacgaa gtttaaatgc acagctgcag catggcgcat aagtatttcg
     2641 ccaacaaata ggactaacaa tagaggaatg attttattca tatggatctc cttctttctc
     2701 ttaggttatc gggtgttgcc cgcttatttc gcaccaccta agcggcgggg gcggttggag
     2761 cgattggtgc gaaataagaa ccattccctt agttactagg ttttttgtcc ttatttcaca
     2821 cattctcact tttcggtata aatgattatt gccttttttt cctttcaaat cgttgatatg
     2881 gcattcgttt acggtcaagt tcattttgga tttcttctgt atcgtcgtaa gtataggtgt
     2941 ctaaaaaatc agttaaagtc gtgtaccagt cgtcgcctgg gttatctact tcttctacat
     3001 attccctcaa aaatgcttcg ggtacagcga gttcataatc cattcccatt aacacgatag
     3061 attccgttcc acacgtcaaa aatctagtaa atacaaggta ataatcgtct atcgtcgcat
     3121 ctttgctgtt tagctcttcc atttcttctt ccattaattc catgctctca aaaattgaat
     3181 aatattctga ttctgagtaa tagtgtttcc tgcaaatcgt gtagctgaca aagttttcga
     3241 ccagctcact cctttctagg taaacattca tgatgtattt ttctgtttct agtcgtagaa
     3301 gctccgttcc tttttttacc gtttcaggac gacgtttata cacgtctaaa cgattatggc
     3361 tgactgtgac cttcgtttga ctcgtttcac tttccaaata caattgttct tc
//
LOCUS       DAYLZO010000192         3367 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.198, whole genome shotgun sequence.
ACCESSION   DAYLZO010000192 DAYLZO010000000
VERSION     DAYLZO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.198"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            3..587
                     /locus_tag="ACGVJG_002948"
     CDS             3..587
                     /locus_tag="ACGVJG_002948"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFJ6558840.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(664..1839)
                     /locus_tag="ACGVJG_002949"
     CDS             complement(664..1839)
                     /locus_tag="ACGVJG_002949"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFJ6558841.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1921..2301
                     /locus_tag="ACGVJG_002950"
     CDS             1921..2301
                     /locus_tag="ACGVJG_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFJ6558842.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2375..2737
                     /locus_tag="ACGVJG_002951"
     CDS             2375..2737
                     /locus_tag="ACGVJG_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558843.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1150 a          554 c          638 g         1025 t
ORIGIN      
        1 atatggcatt cccacaacta gatttaaaaa gtgaaaaagg accaaaagct gttatcaaaa
       61 caaaccgtgg tgacattact gttcaattat tccctgaact agcaccgaag acggttcaaa
      121 atttcattga actttccaaa aaaggttatt atgatggcgt gatttttcac cgcgttattc
      181 ctgatttcat gatccaaggc ggcgatccaa ctggaacagg aatgggtggc gaaagtattt
      241 atggcgaaag ttttgaagat gaattcagtc gtgaactgtt caaccttcgc ggagctttat
      301 ctatggcaaa ttcaggtcca aacacaaacg gaagccaatt ctttattgta aataacacaa
      361 atgtgccagc taatatgctt ggacaactag aaggtgctgg attcccatca gaaatcattg
      421 aagcgtataa aggtggcgga actccttggc tagatttccg acatacagtc tttggacatg
      481 tactagaagg gatggacaca gtagatgaaa tcgcaaatgt acaacgtggt ccacaagacc
      541 gacctgtaca tgatgtagtg attgaaacaa ttgaaatttc agaatagttt ggtatggtac
      601 ggagtaatta tgaaaaagga cgaaacctag taaggtctcg tccttttatt ttgaataaat
      661 gatttaatcg attgtctcta aagcatactt gattcgatta agtccttcca gtaaagtttc
      721 ttctggacaa gcaatattca agcgcatatg cccatgaccg gaaggaccaa acgtgatgcc
      781 aggatttaat acgacttttc ctttccgaac aagagtgttc tccaattctt ggtcgtctga
      841 aatataggcg gtaaaatcta accacattaa gtaagttcct tcagggttca ttattctcac
      901 tttcggaaga tactgttcaa aaaactgatt aactgtttga acattttttt ctagataagg
      961 aatcagttgg cttaaccatt ctccacccgt ctcataagcc gcttgggttc caatcagtcc
     1021 aaaagtgttg atttcgtgct gttggttcat cactaaatgt ttttcaaaat cagctttcat
     1081 ctctggattt ttgataaata ccattgaatt tttgattgca gctagattga aagtcttggt
     1141 tgcggaagta aaagcaacta gtagatcatc taaattagga tcaatcgtta gcatggaagt
     1201 aaatgtatga tcgaataaag ttaaatcttg atggatctcg tcactaaaca gaatcacttg
     1261 atgttttaaa cacaattcgc ttaactgttt caattcttct ttgctccaaa cacgccctcc
     1321 aggattgtga ggattgcata aaatcattgc ttttacattg ttttcttcga tttttttctc
     1381 catgtcagac aagtccatga tgaaatgatt atttttttct agcaaagagc ttcgaacgat
     1441 gtgacgttga tttgtttcta taatagagga aaatggagga taaactgggt catgaatcaa
     1501 aatagaatcg cctggttttg taaaagtttg tactgctgca gctaaactgg ttaacacacc
     1561 acttgtaaaa acgatatttt ctttcgtcaa ctccacagca tggtgttggc gttcccaatg
     1621 aatgatagcc tgatatagtt tatcagacag agtggcataa ccaaagattc catgtttgat
     1681 gtattcacta aacgctttag ctaccccacc gggagacaag aagtccatat ctgctaccca
     1741 caaaggaagt aagtcatcca tttgatatgt ctcagcaatt gcgtcccatt ttacactttc
     1801 tgtatctttt cttgaaatct gtttatcaaa ttcgatcatc ttaagacctc cagtcatttt
     1861 cgtcgcttct ttttttgagc tatttacagt ataatgataa atgcgaagag agggaagtag
     1921 atgacttata aaattggaca aataattgaa ggaaaagtga cagggataca gccttatggc
     1981 gcattcgttt ctcttgataa cgagacgcaa ggacttattc atgtttcaga agtacaatcc
     2041 ggttatacga aaaatatcca ttcattgtta aaagtcggac agccagtaac agttcagatc
     2101 atcgacattg atgaatattc caaaaaaatc agtttatctt taagaacgtt agaaaaaaca
     2161 aatccagctg ttccatatcg cagaaaaagg tatttcacga ataaaaatcg taagatcgga
     2221 tttaccacta ttgcggagca gttacctatt tggatagaag aagcattatc tgaactagaa
     2281 cggaaagaaa ataagaaata aaaagtgtat tttttctgaa aatgtgctag aatttttatg
     2341 taagccatag aacaagttga tcgaggtgga aattttggca agcaaaagag ttgctggtac
     2401 aattatgttg catttggagg atggtactaa aaaatttttg gtccattcaa ttgatgacaa
     2461 gttggaattt gcgttagcgg aactttctga aggaaagact ggtttagcga atatacttaa
     2521 tttcttaaaa gaaatagttc atatcgatgt tagcaagatt agtttgatgg aattgacaaa
     2581 tacacatatt aaagaggaaa atgttccgtt gtttgtcttt gaaactgaac aaaagaatca
     2641 aagagaagaa ttaggcgaag gatatatttg ggaagaacca ggtcaaatgc gctctgttct
     2701 tggaacttac gaagtagagg gagtgccttt cttctaaaat acagattaag cgaacaaaga
     2761 agtttgaatt tttttcaaac ttcttttttt attgtttggt ccaaaaaata caatgaaatg
     2821 ttctgctaaa gatagttttt tagtgcattt tattttcaaa atatgttgaa aacggttgta
     2881 taacgaataa taataattga tcgaaaaatc atttcacaaa gtaatgctta atttatagtt
     2941 gaccaaggaa gaaatacatg ataaatttaa cagcgttgat tctgaagtgg atgttatatc
     3001 acatttctaa aaaatttaaa aaatagttat tgacatgtta ctaacgacat gataagataa
     3061 ctgagtcgtt aaaaaagaac cacttgcgaa gcttgattct atcgaaaaaa acttcaaaaa
     3121 agttcttgac aaataacaaa cgataagtta tgataaatga gttgctgaaa gatttcgaaa
     3181 aacgaaatcg aaaaaaactt ttaaaaaaga gttgacaatc gcttgtctct ctgatatgat
     3241 ataaaagttg ctacggcagc caaaaacgaa tcgggaaaca ctcccgatga agtagacctt
     3301 tgaaaactga acaaagtaag acaaaccaaa tgtgtagggc gtcttgattc aattcaagac
     3361 aacaaac
//
LOCUS       DAYLZO010000193         3352 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.200, whole genome shotgun sequence.
ACCESSION   DAYLZO010000193 DAYLZO010000000
VERSION     DAYLZO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.200"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..1450)
                     /locus_tag="ACGVJG_002952"
     CDS             complement(<1..1450)
                     /locus_tag="ACGVJG_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFJ6558844.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGE"
     gene            2178..3146
                     /locus_tag="ACGVJG_002953"
     CDS             2178..3146
                     /locus_tag="ACGVJG_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6558845.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            3164..>3352
                     /locus_tag="ACGVJG_002954"
     CDS             3164..>3352
                     /locus_tag="ACGVJG_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558846.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTA"
BASE COUNT         1021 a          686 c          646 g          999 t
ORIGIN      
        1 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
       61 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
      121 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
      181 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
      241 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
      301 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
      361 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
      421 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
      481 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
      541 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
      601 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
      661 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
      721 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
      781 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
      841 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
      901 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
      961 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     1021 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     1081 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     1141 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     1201 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     1261 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     1321 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     1381 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     1441 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     1501 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     1561 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     1621 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     1681 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     1741 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     1801 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     1861 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     1921 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     1981 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     2041 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     2101 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     2161 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     2221 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     2281 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     2341 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     2401 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     2461 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     2521 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     2581 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     2641 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     2701 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     2761 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     2821 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     2881 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     2941 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa
     3001 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     3061 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     3121 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     3181 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     3241 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     3301 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag ct
//
LOCUS       DAYLZO010000194         3281 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.201, whole genome shotgun sequence.
ACCESSION   DAYLZO010000194 DAYLZO010000000
VERSION     DAYLZO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.201"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..387
                     /locus_tag="ACGVJG_002955"
     CDS             <1..387
                     /locus_tag="ACGVJG_002955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFJ6558847.1"
                     /translation="ELEAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQV
                     VILYALTHGFLDSIPVDSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQ
                     EYKDMFLATKGSNSSTEDKLKSIQNA"
     gene            401..1303
                     /locus_tag="ACGVJG_002956"
     CDS             401..1303
                     /locus_tag="ACGVJG_002956"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFJ6558848.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            1326..2732
                     /gene="atpD"
                     /locus_tag="ACGVJG_002957"
     CDS             1326..2732
                     /gene="atpD"
                     /locus_tag="ACGVJG_002957"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFJ6558849.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            2746..3168
                     /locus_tag="ACGVJG_002958"
     CDS             2746..3168
                     /locus_tag="ACGVJG_002958"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFJ6558850.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         1082 a          618 c          724 g          857 t
ORIGIN      
        1 gaattagaag catttacaca attcggttct gacttggatg cagcaacaca agcaaaacta
       61 aatcgtggac gccgtacagt agaaatcttg aaacaaaaat tgcatgcacc gctagctgtt
      121 gaaaaacaag tagtgatttt gtatgctttg acacatggat tcttagatag tatccctgta
      181 gacagtattt tagacttcga acatgaatta ttcgaatatc tggataccaa tcattcagat
      241 atctttgaaa caatccgtac aacaaaagat cttccagaag aagaagcgtt gaacagtgcg
      301 attcaggaat ataaagacat gttcttagct acaaaaggaa gtaattcctc tacagaagat
      361 aaattgaaat ccattcaaaa tgcatagtga ggtgagatga atgggtgctt cattaaatga
      421 aatcaagaca cgtatcgctt caacgaaaaa aacgagccaa atcacgcggg caatgcagat
      481 ggtgtctgct tctaagttga caaaatctga agcttcttca cagaagtttc aggtttatgc
      541 aacaaaagta agagaaatcg ttacgcattt gactgctact cagttaaatg atattgcctc
      601 agaaaatcct agaggggata tcaattacaa cagcatgctg ctcagccgtc ctgtgaagaa
      661 aactggttat atcgtcatca ctgcagatgg cggtttagtc ggtggatata acagctcgat
      721 attgaaacaa acgatgtcta ttttagaaga agaccaccaa tcacccgatg attacgtgat
      781 gatcgcaatt ggcggaactg gtgcagactt ctttaaagca cgcggtatca atctagcata
      841 tgagctgcgc aatctatctg atcagccaag ctttgatgaa gtccgcaaaa ttgtaaatat
      901 ggcaacaact atgttccaaa acgaagtatt cgatgaatta tacgtatgtt ataaccatca
      961 tatcaattcg ctaactagtc agtttcgtgt agaaaaaatg ctgccaatct cagacttaga
     1021 tccagaagaa gcaacgactt tcgaacaaga atatattttt gaaccttcaa aagaagaaat
     1081 cttgtctcag ttattgcctc agtatgctga aagtttgatc tacggtgcga tcgttgacgc
     1141 caaaacagca gaacatgctg ctggtatgac agcaatgaaa acagcaactg ataatgctgc
     1201 taatatcatt gatgagctga cggtttctta taaccgtgca agacaagggg ctattaccca
     1261 agaaattacc gaaattgttg ccggagcttc ggcactagaa tagtattgag attggaggaa
     1321 aaaagatgag ttcaggcaag attgttcaag taatcggtcc cgttgtcgac gtggaatttt
     1381 ctttagacca atccttacca gatatcaaca acgcattagt tgtttataaa aatgacgaaa
     1441 ataaatcaaa agttgttctt gaagcagcgt tagaattagg ggacggagtg atccggacca
     1501 ttgcgatgga atcaacagat ggtttacaaa gaggaatgga agtcattgat acaggcaaag
     1561 caatctctgt tcctgtaggt aaagaaacat taggtcgcgt attcaacgta ctaggagata
     1621 cgatcgattt agaaacacct ttcccagaag atgcggaaag aagcgaaatt cataaaaaag
     1681 caccagcctt tgacgaatta agtacaagta cagaaatttt ggaaacaggg atcaaagtta
     1741 tcgatttgct tgccccatat ttaaaaggtg ggaaagtcgg actattcggt ggtgccggtg
     1801 ttggtaaaac cgtactgatc caagaactga tccataatat cgcccaagaa catggtggta
     1861 tttctgtatt taccggtgta ggtgaacgta ctcgtgaagg taatgacttg tattatgaaa
     1921 tgaaagattc aggagttatc gaaaaaacag ccatggtgtt cggacaaatg aacgaaccac
     1981 caggtgcacg tatgcgtgtt gctttgactg gtttgacgat tgcggaatat ttccgtgatg
     2041 tagaaggtca agatgtactg ttgtttatcg acaacatttt ccgtttcact caagctggat
     2101 ctgaagtatc agccttgtta ggacggatgc cttctgcggt tggttatcaa ccaacattgg
     2161 caacagaaat gggtcaattg caagaacgta tcacatctac gaaaaaaggt tcgatcacat
     2221 cgatccaagc aatctatgtg ccagccgatg actacactga cccggcgccg gcaacagcat
     2281 tcgcccattt ggatgcgaca accaacttgg aacgtaaatt aacagaacaa gggatttatc
     2341 cagcggtaga tccattggct tcttcttcaa gtgctttagc acctgaaatc gtgggagaag
     2401 aacattataa agtagcaaca gaagtccaac acgtattgca acgatatcgt gaattacaag
     2461 atatcattgc tatcttaggt atggatgaat tatccgatca agaaaaagtg ttagtttctc
     2521 gtgcccgcag agtccaattc ttcttgtcac aaaactttaa cgtggctgaa caatttactg
     2581 gtcttccagg ttcatatgtc cctgtagaag aaacagtgaa aggattccgc gaaatcttgg
     2641 aaggcaaata cgatgatctt cctgaagaag ctttccgaag tgttggccga atcgaagacg
     2701 tcgtagaaaa agcgaaaaca ctaggctact agaaaggggt gccccatgga tcaatattta
     2761 acagtaaatg tggtaactcc tgccggtttg gtctatgatc accatgcagc tatcgtcgtt
     2821 gcacgtacga cagctggtga gcttgggatt ttgcctaaac atgcaccaat catcgttcca
     2881 ttgacgattg atgaagttcg tgtaaaacga acagattcag atacccacgt cgattggatc
     2941 gctgttaatg gcggaatcat ggaagttcgt gataatgttg tttcaatcgt tgctgatagt
     3001 gcggaacgtg aacgtgatat cgatgtatct cgtgcggaac gtgcaaaaca acgcgcagaa
     3061 cgccaaatcg aagaagcaaa agcaaaacaa gatacgaacg aattgaaacg ggcaactgtt
     3121 gcgttgcacc gagcaatcaa tcgtatcaaa gtatctaagc actcataaaa tgagatgcgg
     3181 atgtttatcc gtgtttaaga acagtaaaaa gaggttatga cagaagtggt cagcttcaag
     3241 acataagaag gaatttacga aaattgcttc tcaaattttt g
//
LOCUS       DAYLZO010000195         3281 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.202, whole genome shotgun sequence.
ACCESSION   DAYLZO010000195 DAYLZO010000000
VERSION     DAYLZO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.202"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(205..1653)
                     /gene="cls"
                     /locus_tag="ACGVJG_002959"
     CDS             complement(205..1653)
                     /gene="cls"
                     /locus_tag="ACGVJG_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFJ6558851.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1805..2689)
                     /gene="ftsX"
                     /locus_tag="ACGVJG_002960"
     CDS             complement(1805..2689)
                     /gene="ftsX"
                     /locus_tag="ACGVJG_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFJ6558852.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2682..>3281)
                     /gene="ftsE"
                     /locus_tag="ACGVJG_002961"
     CDS             complement(2682..>3281)
                     /gene="ftsE"
                     /locus_tag="ACGVJG_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFJ6558853.1"
                     /translation="FVYVVGPSGAGKSTFIKLMYREEKASKGTLNVAGHDLIAIKNRE
                     VPYLRREIGVVFQDYKLLPRKTVYENVAYAMQVIGRKPRDIKKRVMEVLDLVGLKHKV
                     RVFPSELSGGEQQRVSIARAIVNTPKVLIADEPTGNLDPENSWEIMKLLDRINAQGTT
                     VVMATHNSTIVNTIRHRVIAIENGRIIRDQAEGEYGYDD"
BASE COUNT          972 a          719 c          558 g         1032 t
ORIGIN      
        1 tgctatactg gctttgaaat gtgtctcttt taatagcata tattagctta tttcttgctt
       61 tccccaaaaa acaagagata ccccaaagtt aatttcgctc atgcgagacg ctttctccat
      121 ttctgacgta ctctcagaaa tggccccgtt aaaaaaggac acgagatctc catctgttga
      181 tggttctcat gtccttttta tgttttaaag gagcggcgaa atcaatcttg ctaatccttc
      241 tttcatcttg atgattattc ctcggttttc atacatttct tttgtgagat gcgtagaaat
      301 tttcgagtct tcaaaaaaag cttttcttac acgtgaagcc attcgttcat cataaaccag
      361 tgtattgacc tcaaagtcca agcgaaatga acgaacatcg atatttgctg agccaactga
      421 aacaatgcca ccatcgataa tcatcgtctt tgcatgaata aatccattct catatgtttc
      481 gatccttgct ccatactcga tcagctctgc agcgaaagaa tacgttgccc agtaaacgag
      541 aagatgatct ggtttgttag gtatttggat atgaactttt acgcctgaaa gaagagccaa
      601 cttcagtgct tcatgaatcg atccatccgg tatgtaataa ggcgtttgga tcaatatctc
      661 ccgtttggct aacgaaatca ttttcaaata agtcattttg atttgttcat gttcggtatc
      721 tggtccgctt gtcacgattt gaacagctat atcaccagta gtgtgaatgg acggaaaaaa
      781 tttgggagaa tagcctactt cttttgtatg ctgagaattc cagtccatca aaaaacggtt
      841 ttgcaaggta tacaccgctt ctccgtaaat acgcaaatga ttgtctcgcc aataaccaaa
      901 tttctttacc aatcctagat attcatttcc aacattgaac ccacctgtat atccgatttc
      961 cccatcaata actacgattt tacgatgatt gcgataattg atccgaggat taatatacgg
     1021 aacaaataga ggaaagaaaa atgcgacttc tccgcctgct tttttcaaat tctgaaaaaa
     1081 gctggaagaa acttgtgtgg aaccccatgc atcaagaagg actctgactt ttacccctcg
     1141 tcttgccgcc tctgtcaatg catctcgaac ttcttcacct aatgcatcac tgcgataaat
     1201 ataatactgg aagtggatat ggtcttctgc ctgcttgata tcttcaatca gcgcatcgaa
     1261 tttttcacga ccatctgtaa ataaagtgat ttcattttta gtcgtataga gcgtactttc
     1321 aaataccgtg agcatataga tcaattgttt cacatctact tgtccagtag gcggatgagg
     1381 ataaagccct cgcatcaatg cctgtttgtc ttcttcaatt tccaggttct taccgatttt
     1441 cccttggatc ttcaaatcaa agattttctc tttggaaatc cctcttccta aaaaaatata
     1501 taaaataaat cctacaactg gaatgaacat caaaacgagc agccatgccc atgtttgagc
     1561 agtttccttt ctttctcgaa aaacgatgat aaaggaaagc aaaatattca taatcaaaag
     1621 gatcgtaaaa aaattgtcaa cgataatttt cacggcttca cctcttcttc taccaactta
     1681 agaataccca agttgttttt taaagtaaag ggaaaaaagc cgcttccttc tattctccta
     1741 gccaataaaa aaagaaagct ggaacgaaac tgtccaactt tctcatttta tttatattgt
     1801 ggtattagaa tttcaagaaa cgtctcatag aaatgatcga gccaagtgaa ccaatgatca
     1861 caccaatgcc gaccattaaa agattgattt tccaaacgat attttgtgga tgaatcaatg
     1921 aataatggga acgtaataat gatggattga tcaattggaa aaaccatgca taaccaaatg
     1981 tgatgatcaa aattggaaga attgcaccga tcagaccgat ccagccacct tcaaggaaaa
     2041 acggccagcg aatatatcca ttttttgcac cgactaaacg catgatttga atttcacgct
     2101 gtctagacaa aatcgtgatc cgaatcgtgt tcgaaatgag aaacatcgct acgaacagca
     2161 gcaatgccgc tgcaactaat ccccatgtcc gaactgcacc agcgatttgg aaaatccgat
     2221 ctgaggaagc tccaccgtaa tcagaacgag aaacattctt caaatctgct gcttcttctg
     2281 ctacttgttt tgtataagaa ggatcagtag cacttactac atacacatca tataacgggt
     2341 tattgtcacc ttcaaacaat ttccaggaat cacccatttc gttttggatt tttttcaatt
     2401 cctgttcttt gcttgaaaat tcaacttttt taacatgatc taagtcattc aattcttttt
     2461 ctagcttttt catctcttcg tcttttgtac cgatatctac gaatacagat acatcaacgt
     2521 ttccttctat atcctgagct aatttagttg cattcatgat aactgccatg aatacaccga
     2581 ctaaagtcaa cgtgatcgtt accgcactga ctgaagcaac tgtcatccag ccgttacgtt
     2641 tcaaactctt gatactttct aataaatggc ggaaaaaggt tctaatcatc gtatccatat
     2701 tccccttccg cttgatctcg aataatgcgg ccgttttcga tagcaattac tcggtgccga
     2761 atcgtattta cgatcgtact gttatgtgta gccatcacga cagtagttcc ttgggcattg
     2821 atccgatcta ataatttcat gatttcccat gagttttctg gatccagatt tcctgtcggt
     2881 tcatccgcga taagtacttt aggcgtattc acgattgccc gagcaataga gacacgttgc
     2941 tgttccccac cggataattc gctaggaaag actcgaactt tgtgtttcaa accaacaagg
     3001 tccagcactt ccattactcg ttttttgata tctcgaggtt ttctgccgat aacttgcata
     3061 gcgtacgcaa cattttcata tactgttttt cttggtaata atttatagtc ttggaacacc
     3121 acgccgattt ctctgcgtag ataaggaact tcgcggtttt tgattgctat tagatcatga
     3181 cctgcgacgt tcaaagttcc ttttgaagct ttttcttctc gatacatcaa tttgatgaat
     3241 gtagatttac cggctccaga cggaccaacc acatagacaa a
//
LOCUS       DAYLZO010000196         3267 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.203, whole genome shotgun sequence.
ACCESSION   DAYLZO010000196 DAYLZO010000000
VERSION     DAYLZO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.203"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            238..2136
                     /locus_tag="ACGVJG_002962"
     CDS             238..2136
                     /locus_tag="ACGVJG_002962"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFJ6558854.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2435..3154)
                     /locus_tag="ACGVJG_002963"
     CDS             complement(2435..3154)
                     /locus_tag="ACGVJG_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFJ6558855.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
BASE COUNT         1080 a          591 c          656 g          940 t
ORIGIN      
        1 tatagcgaag gcggattatg gagttcattg aaacaatcga tttcaattat gagaaatgaa
       61 cgaaaaaaag cataaaatat agaaaaatat agaaggaagc tggacattta ttgttcccag
      121 cttctttttt gtatagacct atttctattt ttttattata ttggatatat atattcattg
      181 aaaatagagc ataacaactt tataatgttg agtataccaa tttttggagg cgttttaatg
      241 gaccgctatt tagatttact ttcagagaag ttccctacaa cggaagatgt catcacggaa
      301 atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt tttgagcgat
      361 atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg aaatcttaaa
      421 gaaaagatca aagacctttt ctctgaacaa atgacggaaa cggagatcag ccaattcact
      481 atttttacgg cttatccaga atatgtgttg acgactgatt ggtacaaaaa acaagataaa
      541 tcccagctga tccatcgttt gatcgatctt ttacggttta cgacagtcaa atacactcga
      601 tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga actcttgtat
      661 atcgatgatc gtatcaacgg gaaaaaagac tatgtaaaaa aaatcattga acaacttgtc
      721 ttgatgaagg aagaagaaaa atttttaaca aaacttgcgc agacgatcca aaaatctgtc
      781 atcgatcatt tgcatatcgt gggtgatatt tttgatcgag gaactcaagc agccaaagta
      841 atggatcgaa tcatggattt ttcctctgta gatatccagt gggggaatca tgatattttg
      901 tggattggtg cgtattgcgg ctctgaggca tgtttattga cgcttttacg gattgccgca
      961 cgctacaact atttatttga attagagaaa gaatatggat taaatttacg tccattattt
     1021 tcttttgccc atcaaaccta tcagaagaat ccagtattta cccctaaaaa caggaaagat
     1081 ctatctgaaa gagagcaaga agaattagaa aagatccatc aagcactttc tatcctccaa
     1141 ttcaaatcag aacatcagtt gttgaatcga cgacctgaat ttaaaatgga cgatcgtaaa
     1201 ttgttggaaa aaatcaacta tgaggaatca acgatcgttt tagaaggtca gctctatgag
     1261 ttgaaaggga cttgttttca aacgatccag ccagatgatc ctaaaaaatt cttggaagag
     1321 gaacaaaaag tgatcgatag tttgatgtat tcattccagc actcgttaag aatgcaaaaa
     1381 cacatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca gcatgtactg
     1441 ttccatggct gtatacctgt agatgaaaag gggcagctct tagcatttga attagaccaa
     1501 tcttatcgcg gaaaagaact gctgggattt tttgaaaaac agataactga aagcgcaaaa
     1561 gatttgactg caaatgaaga tttggcgacc gatttgattt ggtatgcgtg gagcgggcca
     1621 ttttcgcctt tattcggcaa gagtaaaatg gcaacttttg agcgctattt tctcacagag
     1681 cctcatacgc acgtcgaaaa aagcaatccc tattatcact tgcgagatga agagtggttc
     1741 agtgaaaaaa ttttggcaga attcgatgca aaagaagaag gttcagcaat catcaatgga
     1801 cacacgccag taaaagtaaa aaaaggagag tcaccgatca aagcaaacgg aaaagcattt
     1861 gttattgacg gaggtctatc caaagcttat caaaaaacaa ccgggattgc tggctactca
     1921 ttactatgca attcttacgg tttccaaatc gtgacacatt atccgttctt gcctattgaa
     1981 caacaatttg caaaaaaatc tgatcaaacg aatgtaaaaa aagtgatcga gcagcagctc
     2041 ccacgcaaac ttaatcggaa tacgacaaaa ggaatggaat tacagcaaca gattactcaa
     2101 ctccaaaggc tgttggatta tcgaaaggac gattgagaaa aatgctagcc aacattgctc
     2161 aatagactaa aaagcgactg caacaaaagg cgtgtcgcag tctccttcgg aattagctca
     2221 aaaaacggta aaatattaga agctattttt ctgtttattt ctctaatact caggtcaggg
     2281 cgctgatttc acgacctctt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt
     2341 cacaatatcc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg actcagagtt
     2401 gaacggcagg ctcacgacct aatcagaaaa acgattaaaa ttcattcaca aagaaatcga
     2461 ataaacgctg ttcagatgaa tctacacgat gagaaagttc cggatgccat tggacaccaa
     2521 ggattttgtg catattatct ttactttgga ctgcttcaat gagtccatcg ttggaaaagg
     2581 cgatggcttt taacaaagga gaaagatctt tgactgcctg atgatgatag gaattcacaa
     2641 agtatgaatc agggagaagg tcacttaata aactgtccga aacgattttg acttcgtgag
     2701 ttgcaaactg cggggcagtg ggctgttgtt cgtgttttac ggaccatttt ggatataatg
     2761 aaagatcttg ataaagtgtt ccctccaaca cgacattcaa tagttgaagt cctcggcaga
     2821 ctgcaaaaat cggttttctt tgtttcaatg cttccaagat caatgcctgt tcgaattggt
     2881 ctcgttgaat atttgtttcc gtcagttttg gatgagggtc ttccatgtaa agttgcggac
     2941 taacatcctg accgcctgca agaatgagtt tgtcgatttt ctcaattagt tgaggcgctg
     3001 aatctggaga catgactggc agaataagag gtaatccttg agcatctaaa actgcatcca
     3061 caaatccctg cggtgtatag cttacttgat ttccttggaa agtatctgtc gctcgaatca
     3121 gttgattccc ggcaatgccg atgattggtt tcatttttat tccccctaaa gtagactaat
     3181 tgtaacaagg atttaaaaaa atttaaacta tgtaagactt gcccctatac ttatctgttc
     3241 ttgctgtgtt actatctgta aagagaa
//
LOCUS       DAYLZO010000197         3258 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.204, whole genome shotgun sequence.
ACCESSION   DAYLZO010000197 DAYLZO010000000
VERSION     DAYLZO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.204"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(286..572)
                     /locus_tag="ACGVJG_002964"
                     /pseudo
     CDS             complement(286..572)
                     /locus_tag="ACGVJG_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(749..1927)
                     /locus_tag="ACGVJG_002965"
                     /pseudo
     CDS             complement(749..1927)
                     /locus_tag="ACGVJG_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1957..2367
                     /locus_tag="ACGVJG_002966"
     CDS             1957..2367
                     /locus_tag="ACGVJG_002966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558856.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            2448..3068
                     /locus_tag="ACGVJG_002967"
     CDS             2448..3068
                     /locus_tag="ACGVJG_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558857.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
BASE COUNT         1094 a          489 c          584 g         1091 t
ORIGIN      
        1 ctgttgccat tccattaatg aaaaacgaga aggaagatga ctttaaagta gtttagtatg
       61 aaggtcgatt gataaaacga tgtggattaa tgctaaataa tgagtaattt ttcaataata
      121 caaaaactgt gtacagtatt agaagtagag aaacgctagc gtcgaaaaaa agatattttt
      181 cgtgaaaagt aataggggct aaaaagaaag tatacaattt atttatgttt ctaagcatat
      241 aaaaaagagc gttgaaagga atatttcccc ttcaacgctt cttttttaga agattttaat
      301 gaccatctca acttcatttt caggtttagg taattcatgg aagcttgtgt ttatcgtgct
      361 taattgatca actaaccatg ttttactttt tgactcgtag acaaatctaa aattaatatc
      421 attgttctcg tgttgaagag gtttctccaa ataatctggt gaatattcat agtcaacgta
      481 acctgctaat acaaaagaaa gcagtccatt atttagtgtc agatttaacg gatggtcgta
      541 agcttttatt tttttaatgt catattgtac atcttatgaa gacttaatga ctacttgtag
      601 tcaatagatt taaagtattt attttctcat ttatgtatca agaataaata ctgatataat
      661 aaaaattata tcaatgtttt gatgaagtaa tttggaggga gcgtcaataa ttttgtgtaa
      721 ataactcgtc cttctgcaaa ataatgggtt actcagtaaa cattgaagct aatgtatccg
      781 tcacttgttg gaagccttta tggcttctat ttaagaattt ttgattgtat gtatcaaaga
      841 tggatactag gaaacgctct agtgattctt cattttgaaa ctgctctttt ctgcggctgt
      901 atttcttgat ttgtttcttg aaagattcga ttaaattagt tgagtaaatg cttcggcgga
      961 taccaggcgg aaactcataa aaagtcaata aatcttgatt ttttatcagt gactgcgtca
     1021 ctttaggata agttttttgc tacttctcaa tcatactccc taaaaaggta tttgcctctt
     1081 cctttgagct agcttgataa acagccttaa aatcatcaca gatttctttt cgatctttga
     1141 cacgtacttt atgagcaata ttacgggaaa cgtgaacaca gcaatgttgg tatttagctt
     1201 taggataaat ctggtgaatc gtatctttca tgccttttaa gccatcggta ataaacagta
     1261 agacatcatg aacccctctg gagtaaatgt cctgtaacaa ctcattccaa acgtaggtcg
     1321 attcagtcgg agcaatcgca taacttagta cttctttagt gccgtcttct cgtataccaa
     1381 tggcaatata gattgcttct ttggagacgg tttgccgttt taaaggaata taagtagcat
     1441 ccatgaaaat agcgacatac ttatcattta gagttctaac tttaaatgca tttacttctt
     1501 cagtcagcac tttagtcatg ttggacatgg tttggggcgt gtagtgatgc ccatacattt
     1561 tctcaatcaa gtctgcgatt tccgacatgg taataccttt tcggaagagg tgaatgacgg
     1621 tctcctctaa ggtatcattc gtccgtctat aagcaggaac agtctgttgc ttgaactcgc
     1681 cgttgcggtc gcgcggaatc tggagatgaa gttccccgta ctcggtcttg accgtacggt
     1741 cataggagcc gttacgagaa ttccccgtgt taaaaccaat gcgatcgtac ttttcgtaat
     1801 cgaggaaagc cgttaactcc gttttgagaa gcgtattgac agctttttcc aaatggacac
     1861 ggaataattc attcaaatcg cctttgttcg ctagagtctt tagaatttct gtagtaaaat
     1921 cgttcatatg gaagtcctct tttctgtgaa tgtgttgtgg taactctatt ctacagaagg
     1981 gacttccttt tttctattta cacaaaatat tttacactct cgtcaaatat aaaaactagt
     2041 acgaaattaa tttgtaaatt atttaagctt gataaaataa gcaatatcga tgatgtaaag
     2101 tccaagatat taacaagaaa agtaagcgaa ccagtaaagc taacggcaca agataaaaaa
     2161 aatggaattg ttctttatgg tgatgatgca aagtataact ataattggga tacctcctca
     2221 gaagctgagg tattaagtat tcctactacc gatacttcag gggaaaaagt cacctttaaa
     2281 ataaatttta taacggcaat ttctaagttg gagtatggga agcaaaaaaa gagagataac
     2341 aatgatcaat atctacctaa aaaataacga attaatgatg catatgttaa agttgttttt
     2401 ttgaattaaa caactcaatt tattcagtta tatgttcatc gcagcaagtg aacattaatc
     2461 gagttataaa attaatagga gctaaaaata taaataaagt tcgatcagaa tttatttttc
     2521 cagaagattt ttttaactgg tttttttatc aattcagtga atgcaagcga ctcttaggtg
     2581 agaattatta cttaaaagga atcggcggat ttatggggaa tgtggctgat gaacaaaata
     2641 ttataaaagg aatatctgaa caaacatcag agcttacagt aactaaagca tttataagta
     2701 atggtgaaag tttgaaaaat gtaactgcgc gaattagaaa agaaaatgaa gtagacattg
     2761 tttttggaat tgaccatgaa tgtaattcag tgatatatgt aacacaatca gcaaatttaa
     2821 ttctttttga gaatggttgc aaggacgatt ttatggtggt ctatttgtac gatatactaa
     2881 ttccaaaact tcgttcctta tatatgtctt ctgaaagtat ttttgtgagt gaacaaaagg
     2941 gatctttctc gaaaaaaata ggtattgaag taattgaatc gattatgtgt cataatcata
     3001 ttacgctaga agaattagaa attttgatag ggcaaaaaaa taataacacg aaaatacaag
     3061 tttcttagaa atcatttttt attgagatat ctagataaat gatttattct tcaatcaacc
     3121 aactcgccaa gctgaggtaa tgttttgagg taatgataac atacaacttt ttatacccta
     3181 gtaaaactct ataaacaaag acttattaat ttcgcatgaa atacattcct gtaaaatgca
     3241 ataaaataga gttttgtt
//
LOCUS       DAYLZO010000198         3234 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.205, whole genome shotgun sequence.
ACCESSION   DAYLZO010000198 DAYLZO010000000
VERSION     DAYLZO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.205"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..588
                     /gene="rnhC"
                     /locus_tag="ACGVJG_002968"
     CDS             <1..588
                     /gene="rnhC"
                     /locus_tag="ACGVJG_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFJ6558858.1"
                     /translation="AYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLV
                     LSPEKYNEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYAR
                     LEKNQLQEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSD
                     QVAAKLLEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(676..1587)
                     /locus_tag="ACGVJG_002969"
     CDS             complement(676..1587)
                     /locus_tag="ACGVJG_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFJ6558859.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1988..2566)
                     /locus_tag="ACGVJG_002970"
     CDS             complement(1988..2566)
                     /locus_tag="ACGVJG_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6558860.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(2586..>3234)
                     /locus_tag="ACGVJG_002971"
     CDS             complement(2586..>3234)
                     /locus_tag="ACGVJG_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558861.1"
                     /translation="EVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGSGKSTFLT
                     LAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTVKKQLLLV
                     DKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPSIILADEP
                     TASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVLKEKE"
BASE COUNT          950 a          658 c          533 g         1093 t
ORIGIN      
        1 gcttatgtga aacgtgatca gcttgcagaa ttacgtaaac ttggtgttag agattcaaaa
       61 gagttaaaag atagccaaat catccagctt gctgacatct taaaaaaaac catcccctat
      121 aaactgcttg tcttatctcc agaaaaatac aatgagatcc aaccaaaata caatgccgtt
      181 cggatgaaag tcgctttgca caatcaagcg atccatttgc tgctaaaaaa aatcgagcca
      241 gaaaaaccag aagcgatttt gatcgatcag tttacgccgg aagcaaattt taaaaaatat
      301 gcgcgtttag aaaaaaatca attacaagaa aaactgtact tcgttactaa aggagaacaa
      361 tatcatttag ctgttgctgc tgcttctatc atcagtcgtg ccagcttttt ggaagagtta
      421 cacaaagcat cagctgaagc tggaatcacc cttccatcag gagctggaac gaaatctgac
      481 caagtagcgg caaaactatt ggagaaaggc ggcatgccta tgcttgaaaa atatgccaaa
      541 cttcactttg caaatacaga aaaagctttg aagctactaa aaaaataaac ggtgttcaaa
      601 agcttgcatc tgcgataata agcccggcca aacgaaaaat atgaggagct atttttgttt
      661 ggctgggctt atttattact gaaaataagt ggtcaatgct tgtgtcaaac ttgcagcaat
      721 ctgctgctga taactgtctg ttactaattc ctttaaatca ctatcattat tcatataacc
      781 catctcgatt aaaatagaag gttgaagatt ttctcgaaga acttgatagt tcccagtgga
      841 gattcctctt gaatccagag gcagatcagt gaaggagtca gcaattgtct gtgctaatgt
      901 ttcctcttgt ccatagtaat aatatgtcgt gattccagtt gcttcattcg catattccgt
      961 agagtctgcg tgtaaactga taaagacatc tgcctttttt tcttcactgt agtcgcatat
     1021 atctccaagc tgtactaatt catctgtagt atgggtcaaa taaaccgttg ctccagcatc
     1081 ctctagcgcc ttttttactt ttatggctgt acttaatgtt atatcttttt cttctgtttg
     1141 ttcatcattg cttaatgccc cagtatcatt tccgccatgc ccaggatcta atacaatcac
     1201 tgcatcagat agctcttccg gttttgtttg tatattcttt gtgactttgg ttccatcttc
     1261 gagggctaaa tatccagtat agccatcatt tgtcttgatc tgataagcat tttttgtttc
     1321 agaaagaaac tccgccagct ctccgccgtt tatagttgca agtggggtag ttagcgagtc
     1381 atctgtatat agtgtacggt cattttttcc gattttgatt aatgtctctt gttctgtatc
     1441 atcaaatgat tcctttgtct ttgtaaccgt actgttcgta ctgctgaccg tttgtccaac
     1501 tatctttttt tgaccaaaaa catctatttg aaaacagata ataatgataa tgattgcaaa
     1561 tacaaaagct ccaattattt ttttcattct tgactctcac ctcgtagatc cagactaagg
     1621 gccaaaactt aaaatcagct taagtaagca aaaaaggggc tgtaaaagat ataaccgtag
     1681 aaaataacat tactgctaaa atgatttttt tcatttttgg catatcctga aatctccttt
     1741 tgactattcg tggtgattca tctacccaat aaatagtttc cttcattcta caaacaatta
     1801 atttcttcgt tattaaatta aaacacttcc tggaacaccg acataataaa taaaaatata
     1861 ccctgataat catgagagaa taacaaattt gtctttatga aaaactacct atataaagaa
     1921 gcaagatata acgatcttcg tcatatcttg cttcttcacc taactatttt ctccctatat
     1981 aacagcttta cgcttctcct aatacaccat tcaaatataa atcgatcaat tgcgccacat
     2041 ttagcggttc ttccacaggt cttttcgacc gagtgatgta aggtgctaac actaagaaaa
     2101 aatgccttgc tgcgatttct tttgaaatcc catttcttag cacagattgg tttgctgaaa
     2161 agatttcttc aaaaggttca cgataatttt tcttccatag aaagaaaaga tgatctcttg
     2221 tttcctttga aagattgctt tcaagatcgt gaagcatcag aaacaaatcg accagatgtg
     2281 tctcaatcaa aaactgcgcc atttttgtca aggttacttg aaatcccgtt tcgcttgttt
     2341 gaacgatttc tctcaaagct tttccaacat cttttaagtg gttttctaaa actgccgtat
     2401 atagtttttc tttatcaccg aaataatgat agagattggg ctgggtgatc ccaacttttt
     2461 gtgcgatttc tcttgttgaa gtgccgagat atcccttgga cataaacaag tcagtcgctt
     2521 ctttcaaaat tttttgctgt gtatctttgt tgtatcgatg gaccattttt ccacctgctt
     2581 tttggttatt ctttttcttt caatacacca tcttccatca taaatattct atcgcatttg
     2641 tcaatcagcc gtgtatcgtg agtaaccatg atcgttgctt tatttttgtc ttttgattct
     2701 tttgccaaga tatcgactac ttctcgtgcc ttttctgaat ccaaactagc agttggttca
     2761 tctgctagaa taatggacgg atcattgtac aatgcacgag cgattgcgac cctttgccgt
     2821 tctccaccag atagttcttc tggatacttg ttgatcaact tatccacgcc aagctgctca
     2881 aataattcgt ttgcttcttt ttgacgatgt tcatgtttaa tcttatctac taataataac
     2941 tgctttttta ctgtcagaaa aggaaccaga ttggaagctt gcaaaataaa gccaatttct
     3001 ttaaaacgta gtttaacgcg ttcgctttct ttttgattac tgaattcttg atcattgata
     3061 aatacagagc cactgctagg cgtctgcaat ccgcctgcca aagtcaaaaa agtactttta
     3121 cctgaaccag aaggaccgat gattgcaaca aattcccctt tatcgatact catggttgtt
     3181 tctttcaatg ctttaactac ttggtcgcca tcttggaaac ttttttctac ttct
//
LOCUS       DAYLZO010000199         3219 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.206, whole genome shotgun sequence.
ACCESSION   DAYLZO010000199 DAYLZO010000000
VERSION     DAYLZO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.206"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            1..329
                     /locus_tag="ACGVJG_002972"
                     /pseudo
     CDS             1..329
                     /locus_tag="ACGVJG_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(327..1046)
                     /locus_tag="ACGVJG_002973"
                     /pseudo
     CDS             complement(327..1046)
                     /locus_tag="ACGVJG_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1071..1256)
                     /locus_tag="ACGVJG_002974"
     CDS             complement(1071..1256)
                     /locus_tag="ACGVJG_002974"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFJ6558862.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1483..2756
                     /locus_tag="ACGVJG_002975"
                     /pseudo
     CDS             1483..2756
                     /locus_tag="ACGVJG_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2863..>3219)
                     /locus_tag="ACGVJG_002976"
     CDS             complement(2863..>3219)
                     /locus_tag="ACGVJG_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558863.1"
                     /translation="SRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQF
                     EPVPQGVKIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDI
                     LAIPVEIDTQHFAQQS"
BASE COUNT         1019 a          728 c          570 g          902 t
ORIGIN      
        1 actcagatgt tgaaagtcaa agaaaagttg actattttag aattaaaacg tacccgtttt
       61 ctatgctatg attgtgggca gaccttttct gcgaaaacgg acttagttga tgaacatcat
      121 caactaacaa aggaactgaa acaggcgatt ctcatagaac tgtacgaaaa tcaatcacgt
      181 aatctgaaaa tcagaagaag taccgtcggc tcaagcgata ttggaaactc ttactcaaag
      241 attccacgac attggagccg ctcaaacggc attatcatcg actgtttaaa cgaccaatta
      301 gtcaaacaga aatcgtcgat gaaccgttgt gcacaatggt gtcctgaacg tacagcgaca
      361 ccttccatat ccagagcagt cgccacatca cggggatgga tgccatccaa attaaaagca
      421 accacgccag tatgatgttc aggatcttgc ggtccgtaaa tggtgatacc gccgatcgct
      481 tgtaatttag gtaaaacgta ggcaaccagt tcttgctcat aacgatgaat attctccata
      541 ccaatctcat tcaagtaatc gattgctcct gcaatgttcg gagtaccagc ttcaaatttc
      601 catggcagct ctttccatga actatcgtaa agattgacaa aatctatcat ttcaccgccg
      661 aattcgactg gctccatttg ttctaacaat tgacgtttgc catataatac accgattcct
      721 gtcggcccgc acattttatg cccgctaaag gcgaaaaaat ctgcatccaa cgcttgtaca
      781 tcaactgtca tatgcggtgc tgcttgggca ccgactggac tatcctcaac tacaacagcc
      841 tgttcaaccc tcagccgttt caagcacaac aaataacctt ctggatctgg tttgccgacc
      901 tgaagatcat cgatcgtcac aaaggaatca attttttcct taatcatcgg atgtgcttca
      961 aaaaaatagt ctagctcccg cagttcagaa ttgagactac tccgattttc cagtgctgct
     1021 gatccgctgc tcaaaatgtt cagcatccac tggtctccca accgcttgac ttatcccttc
     1081 ataaaaggac agttctgctg ccgtcccaac accttgttga tagtctgcaa aaggaatttt
     1141 atagccgaac tgctccagta aataggcttc agtaaatagt gatgaatact ctcgtaaaac
     1201 atccattcac tgtcaatcat cgtcccatca aaatcaaaaa taagtgcttt ggtcatcttc
     1261 tttccccctt acccaaaaca agcaaaagct gtgtcctgct ctctctacag tatcagcata
     1321 aaaactgccg tctgaaaagt caaaggtgaa agaaatgtgg cgctataatt tctaggactg
     1381 atagtaaatc attctatcag tccttttgag cataaaaaaa caaaacatac agaagctccg
     1441 ttagaattga atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatctt
     1501 atcaaactat tactaggaat caaagatgga aacattcaaa tcactcatgt cgagaagaaa
     1561 aaaatacatt caatcaacag tatcgttgtt tctgccacgc tcattaaaac gattcagccc
     1621 tgtcctcagt gtaaacaggt gaatcaacat gggctgatcg ttaagaacgg aaagaaaaaa
     1681 tcgctggtcc ttctcaataa gtgtgcgaat caacggactt atcttgcttt ggcaaagcaa
     1741 cggtacctct gtcgtggctg tcacacctat tttacggcga atacctacat tgttgaaaaa
     1801 aactgtttta tcgccaaaca agtacattat aaaatattgg aggaattgac tgaaaaacaa
     1861 gcaatgacga cgatttctaa acattgcggc gtttcttggt caaccgtttc gcgaacactc
     1921 gcttctctgc tccccatgac aaacgtgaaa ataaattggt tgcctcgctg ttcattggta
     1981 gatgaatttc gttcattgaa aaatcaagtc ggtccctatt ctttttcttg tatggatggc
     2041 gatactggga aattactgga tattcttcct tcacgtaaga agaaagatct catttcttat
     2101 tttatgcaat ttgaacgacg tgcacgttta aacgtaaaaa tactcgtaac agatatgaac
     2161 gcctcttatg ctttgctgat aaaagagtgc tttcctcatg ccaaactcgt tgtggatcgc
     2221 tttcacatcg tcaagcacct cattcgcagt tttaaggata tttgactatg agtcatgaaa
     2281 agcttcgacc gaaacgatcc aatacaagcc aaacactatc gacaagtgaa ggctttatct
     2341 cgacttctga tcaaacgaca ggacacgctt gtttatgata aatggacaaa atggcgtaat
     2401 tttggttggg cctatttaac cgaaagtgaa gtcgtagaac gtctactttc tacttctgat
     2461 gaactacgaa tagcttatgc ttactatcaa gaaattcttc aagcttttta tgacaaagaa
     2521 gcggacatct tctttcagct tgtaaaaaca atgcccaagt cggttccaag agaactgcat
     2581 catataaaaa aagcgtttat aaactatgaa tccggcattc gtttagcctt agagttgcct
     2641 tactcaaatg gcaaaataga aaatctgcat acccacatca aagcattgaa acgaatagct
     2701 tacgggttag gatgagaacc agaattttct taatcaatca tctgatcact tattaaacat
     2761 aaaaaaacat ctaaagcaga aaatcaattc taccttagat gttcttatat cactctatca
     2821 gtcctatttg acaaagagcc agaaatgttt gccgactata cactagcttt gttgtgcaaa
     2881 atgctgagta tcaatttcca cagggattgc tagaatatct ttcagctgaa tgtataaggc
     2941 atcgctaatt ttctcttccc gatcgttcaa ccgatccact aaaatttctt tgccctccag
     3001 cgtttgaaaa acataccgtt gaatgttgcc taaaataaat gattccttcc atagaaccgg
     3061 aatcttaacc ccctgaggaa ccggttcaaa ttgaatcagc tccggtctta aataataaat
     3121 gcagcgattg cccttagcgg aaaactcgtg ggtatactga ttcagctcat accattcgaa
     3181 acgattatag ttgccgataa attccgcaac aaatcggct
//
LOCUS       DAYLZO010000200         3199 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.207, whole genome shotgun sequence.
ACCESSION   DAYLZO010000200 DAYLZO010000000
VERSION     DAYLZO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.207"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            301..585
                     /locus_tag="ACGVJG_002977"
     CDS             301..585
                     /locus_tag="ACGVJG_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558864.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACGVJG_002978"
     CDS             588..1004
                     /locus_tag="ACGVJG_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558865.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACGVJG_002979"
     CDS             complement(1141..1860)
                     /locus_tag="ACGVJG_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFJ6558866.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACGVJG_002980"
     CDS             2522..2608
                     /locus_tag="ACGVJG_002980"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFJ6558867.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..>3199)
                     /locus_tag="ACGVJG_002981"
     CDS             complement(2910..>3199)
                     /locus_tag="ACGVJG_002981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558868.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAYLZO010000201         3069 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.210, whole genome shotgun sequence.
ACCESSION   DAYLZO010000201 DAYLZO010000000
VERSION     DAYLZO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.210"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(62..613)
                     /gene="raiA"
                     /locus_tag="ACGVJG_002982"
     CDS             complement(62..613)
                     /gene="raiA"
                     /locus_tag="ACGVJG_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFJ6558869.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(741..1460)
                     /locus_tag="ACGVJG_002983"
     CDS             complement(741..1460)
                     /locus_tag="ACGVJG_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFJ6558870.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            complement(1457..2770)
                     /locus_tag="ACGVJG_002984"
                     /pseudo
     CDS             complement(1457..2770)
                     /locus_tag="ACGVJG_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(2851..>3069)
                     /locus_tag="ACGVJG_002985"
     CDS             complement(2851..>3069)
                     /locus_tag="ACGVJG_002985"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFJ6558871.1"
                     /translation="DIFAVSASDADNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGI
                     PLDVINMADYGMMNGEKVLDQAIALIGE"
BASE COUNT          846 a          649 c          546 g         1028 t
ORIGIN      
        1 aaaacttgcg gcagctgtca gtagctgctg caagttttcg ttgttattta agtaaggctg
       61 atcagtctgt ttcaatcagt ccgtattttc catctttacg gcggtaaacg atacttgtac
      121 catttgtttc tgcatcttca aaaataaaga agttatgtcc cagcatgttc atttgtaaga
      181 ctgcttcttc actatccatt ggtttcaatg aaaggcgttt cgtacgtacg atatctaatt
      241 cagaatcatt ttcgttttcg tcctcttcat tgaataaagc agcagcatct gcagtattca
      301 tgcccgtttc acgggattta cgattaattt ttgttttgaa tttgcggatt tggcgttcca
      361 atttgtcaac aaccaaatcg atacttgcgt ataaatctgg agaagtttct tcggcacgta
      421 atacaagata aggtaatggg atcgttactt ccacttttgc tgttttttct gtataaactt
      481 ttaagttcac atgcgccgtt gcatcgggtg cgtcactgaa atagcgttct agtttgccta
      541 cttttttctc aacgtagtcg cgaatcgctt cagttacttc gatattttcg ccgcgtacat
      601 tatatctaaa cataaaaatt acccctttcg tctaccacaa tcaagaagga gaaggaccac
      661 tctttccctc ttcttaattc tattatatcg aactctaggt tatttgcaaa ttaaatcgct
      721 atcaaatttc cacatctgat tcatcttgcc aaagaaatgg ttcggatgat tttcggctgt
      781 gcgttctgca agcaatctgc tgcatgaaaa agcgtctgtc ctgttgtata gacatcgtca
      841 ataatcaata tttttttatg ttgtatctta gaaagaggta cggcaagctc aaaaggctga
      901 ggcatctcca atctttcctt tcgcgtgttt tttgcttgag caagcagatg ttgctctctt
      961 ttgagcagca tctgaaaagg aatttttgct gctttcaaca tctcgcttac ttgattgaat
     1021 cctctttcta gccacctttt ttcagataaa ggtattggac aataaatata tgccgggtaa
     1081 tcttttttta tctgagtcca tttatatgca aaagtccctg ctaatcggat atctcccata
     1141 aacttgtacc gattgatcca agcctgcatc ttttcatcgt acgtaaaaaa ggcgcggtgt
     1201 tgaaaatcat atgcaggata tttcttcttc cattcttgac agtcgttgca gtaaacgaaa
     1261 ctagtatttg ctttcttttt cgtctcctcg tatttcaaat ggattggttt ttgacaacct
     1321 gcacaacctt ttgcttctaa taaatggaat tgtgcgcggc aaactgaaca tgtctgagga
     1381 agtctagatt tcgacactcc gatgatctcg ctgaatgtta aattttgaat gatgtcttta
     1441 ccgcagcaac tacatctcac agcatctgct tcccttcttc gttcatttct ttgatttcct
     1501 gacaggcttt tcgaatagct gaagtttttt cttcatagaa aaaatagact cgactgttcg
     1561 tataaggacc tttacgatca gctcttcctg cgatttgcac caaagaggat ttagtaaaaa
     1621 ctggatgatc tgctcctaaa acgatgacag agattctttc aaatgtgact cctctttcta
     1681 ggatcattgt ggttaacagt atctcgtata ttgcttctcg cattttctga actttctttg
     1741 cccgttgatc gtcttgtgaa gaaacatctg ttatctcata ttctggaaaa gcttcttgca
     1801 gtgtgatttt taaacgatgc atcatcgtaa tacttggaca aaataaaagg atacggtttt
     1861 ctttgattag ttgctgaatg atccgcacca aagagttgat tttctttttg tttccacatt
     1921 tttgtcgcca attattcatg aaaacaagca cgggttcagg aagcaagcgc cgatgaaagc
     1981 gcaaggcaag tttgtggagc tcaaatgtct gcttgatttc ctttagtaac ttctcatcag
     2041 gcgtggcact taagtagatg aatcggccgt ttgatctgat tgctgtttgc acagcgtgag
     2101 ctagcccagc atccccgtta taaggaaacg catcgatctc atcgatcact attaaatcaa
     2161 aggaccgata aaaacggtat agctgatgaa tcgtacagat aacaaaagaa gtaaaggtat
     2221 aagccgttgc tgtattccca tggagcaacg tgattttttc ttctggaaaa acataacgat
     2281 agcgtaaaaa caattcatta caaacatcga ctcgcggtgt acaaatcgct actcttcctc
     2341 cagcttttaa agtttccact aaaataggat aaagcatctc tgttttccct gcacctgtta
     2401 cagcccaaat aagatgatgt cttttttctg aatggcaaag tattctcttt gcgatttccg
     2461 tttgtttttc agtaagctgt cctgtccaaa aaaagacgac ttctcgaggt tttgcttcag
     2521 gctgatcaaa caaatataaa tcttgttgag tgtcgcatca ccctaaatat agacaatgtg
     2581 tacaaaaata accgtaatct gcaagctgtc caacaatgac ggacgtcctc attccacagc
     2641 gatgacaaag aatccagttg cccaaacgtt ccactgctgc gatcttctca taatttggta
     2701 aatttacctc ctcacagtct attgctttaa tatctctcgt cagaagcctt ctaccccaaa
     2761 aatgattcat attattcgcc ctaaaaaaat atacgaaaaa acaagcgatc tattttttgg
     2821 atcgcttgtt cgatagttat actgttctat ttattcacct atgagcgcga ttgcttgatc
     2881 taacactttt tcgccattca tcataccata atcagccata ttgatgacat ctaaaggaat
     2941 gccttttggt tccagacgtt tctcaaaatc agctttcata aagcgaactt gtgggcctaa
     3001 taacaacaca tccacgtctt tatttgcaag attgttatct gcgtcagaag cggacacagc
     3061 gaaaatatc
//
LOCUS       DAYLZO010000202         2951 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.211, whole genome shotgun sequence.
ACCESSION   DAYLZO010000202 DAYLZO010000000
VERSION     DAYLZO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.211"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            17..2344
                     /locus_tag="ACGVJG_002986"
     CDS             17..2344
                     /locus_tag="ACGVJG_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFJ6558872.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2341..2951
                     /locus_tag="ACGVJG_002987"
                     /pseudo
     CDS             2341..2951
                     /locus_tag="ACGVJG_002987"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
BASE COUNT         1014 a          570 c          588 g          779 t
ORIGIN      
        1 tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc aacgtatatg
       61 tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact tacatcccat
      121 tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta tttatgtaaa
      181 cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa atagtccacc
      241 ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg agacaacctc
      301 aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag cagcaaaagc
      361 agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta ctgttgcggc
      421 cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat atgccaaaga
      481 tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt tacaaggcgt
      541 aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt ttattggctc
      601 cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg tggctaccgc
      661 tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt ttacaccctc
      721 aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg gtatacagtt
      781 agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg ttgatggtaa
      841 gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca atgcgaatcg
      901 gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa cgtacgggac
      961 tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag cagtaacggg
     1021 ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg tcgatacggt
     1081 gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt ttggacagat
     1141 gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag acctgacgac
     1201 taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgatag aaacaaagaa
     1261 ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa tcgtaaagga
     1321 cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg caacagtatc
     1381 aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga tcgttaaata
     1441 tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca gtattgatca
     1501 aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg aagtaactgc
     1561 gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac tagctaaact
     1621 agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct atacgattaa
     1681 gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa aagcaggtgt
     1741 cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc cagtacaagt
     1801 ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata tggttaatgc
     1861 tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag cagcaaatcc
     1921 caagttgtca tttactgata tagccaacac atcaggaact tataccatct ataaagctaa
     1981 cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc actcgatttc
     2041 ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt ataccctaac
     2101 agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag gcgaaggaag
     2161 tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga tcaatccgac
     2221 tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt taggactgat
     2281 ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag gtgaacagcg
     2341 atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt tatcctactg
     2401 gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac atttactttg
     2461 cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga cggaataaat
     2521 atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc tatatatgat
     2581 gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga cgcacaaaga
     2641 aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac aaaaacagta
     2701 gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca aggtcgtgac
     2761 gctgtttacc gatttacgga aataaagact tcagattaag taaaagaaaa atcagcacca
     2821 tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac catccacttg
     2881 tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac agtagagaat
     2941 aagcagacag a
//
LOCUS       DAYLZO010000203         2950 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.212, whole genome shotgun sequence.
ACCESSION   DAYLZO010000203 DAYLZO010000000
VERSION     DAYLZO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.212"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..185
                     /locus_tag="ACGVJG_002988"
     CDS             <1..185
                     /locus_tag="ACGVJG_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558873.1"
                     /translation="SRNRYLKVLIENDFDHELMDHRKDQFDRLLEKFVDIQTYNTKIL
                     EEYIATNNQLIALLIE"
     gene            227..649
                     /locus_tag="ACGVJG_002989"
     CDS             227..649
                     /locus_tag="ACGVJG_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558874.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            669..2261
                     /gene="mobP2"
                     /locus_tag="ACGVJG_002990"
     CDS             669..2261
                     /gene="mobP2"
                     /locus_tag="ACGVJG_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFJ6558875.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2282..2617
                     /locus_tag="ACGVJG_002991"
     CDS             2282..2617
                     /locus_tag="ACGVJG_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558876.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2763..>2950
                     /locus_tag="ACGVJG_002992"
     CDS             2763..>2950
                     /locus_tag="ACGVJG_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558877.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1179 a          439 c          593 g          739 t
ORIGIN      
        1 ggagccgaaa tcgataccta aaagttttga ttgaaaatga ttttgaccat gaacttatgg
       61 atcatagaaa agatcaattt gatcggttgt tagaaaaatt tgtggatatt caaacttata
      121 atacgaaaat tttagaagaa tatattgcaa caaataatca attaatcgcc ttgttaattg
      181 aataataaaa atagtattgt gaatttataa aaggaggaaa tcaattttgg caaaagcaac
      241 aagaaaacat gtatatcttt acgatatgca tatcgaagta cttgatgaaa taatttctac
      301 tcatgaagat atatctaatt atagtgaagc agtcagacat ttgtgtatga tgtatgatcc
      361 aaaaatcgaa gaaaaaataa atccaattca aattaaattg aatgcaatgg gaaaagaaat
      421 ttctatatta accgaaatgg tttcagagtt tgcggatctt catttgaaag atacaggaat
      481 actaattggt acaaattcaa ctgtctatca agaagcaaaa gagcgtgtgg aagggaaaat
      541 aaaagcgaat caaacgaaaa agtattctcc taagatcaag aaaccaataa atgaatctgt
      601 tcatgccaga gtagacccgt ttgatccaac aaagttttta aaaagataaa aagtagaagg
      661 ggaacatgat gaataaatcg cctgcagtca ttctcacttg tcaatttacc ttgcccaata
      721 aaaaatcttt ttcaacatac attgattata tgacaagaga aaaagcgctg gaagaaaagg
      781 aacgacgcac gccggacgaa gaacaggaac tgcaacgaat caagaatgct ttagaggatt
      841 tttatattcc tgaaggagaa actttttcgg agagtaagac agataaagaa ttcagtgaaa
      901 cgtcgaatga agctcaatcg ttgatggaac atggaatcaa ttttgacaag ttagaggaac
      961 aagattttac caaatatctt tcttatatga cccgtcacta tgcgttagaa caaaagaaag
     1021 aactgacgca aagggaacaa aaagaatacc aacggttgaa ccaagcaatg aaaaaatatc
     1081 aaacagagcc acataaaaaa gcttcaggaa atcttcctgg agttttttct atggcttcag
     1141 acgaagtgaa aggaagtgat ctgaaggaga tcaaacaaat cttccaaaga gggcaacaaa
     1201 aaggctcaat catttatcaa gatgttgtct ctcacgacaa ttcgtatctt gaaaaaatgg
     1261 gcttgtacga tcccaaaaca gataccttag atgaagaagg attaaaagca gctggaagaa
     1321 aaatgatgga gacattattc gagaaagaac aattgaatga aacaggttac tggatggcaa
     1381 cgattcaccg taacactaag catatccata ttcattttgc agttgttgaa aaagaaaata
     1441 cgcgtaaacc ttacacggag gttgaaaatg gtgtgagtta tatcgtaccg aaaggtaaga
     1501 gaagacaagc aacattagac gcaatgaaga ccaattatgt acatgagtta gaacgttttt
     1561 cttatgaaaa ggatcgaatg attggtatgg agaaacgaaa tttattgacg cggaaaagtg
     1621 atttgcgtaa caccttaaca aaagtggtaa aagagccagc gcgttacgat cgacacgcaa
     1681 tgaacttatt aaatgaagta tataaaaccc tacctgacaa acgttcggaa tggcattatg
     1741 gggatgaaaa acgaaccaaa ctttcaccat acacaagaga gaaactcgat gagttaacca
     1801 aatatgtgtt aacaaaagaa cctgaatatc aagaatatat acagatttca aaatcattga
     1861 aagaagaagc aaaaagtctt tatggggaaa gcaagagaga atcaaaggat gcagaaaaaa
     1921 atgctctctt tgatttgaag aagcgcacag gaaatgccat tctaacagaa ctgaagaaac
     1981 aggatagtca aataaaacct ttaatagaaa gtatggatca gatgcaatct atttacaatg
     2041 aacgatcctt tcgaacgctt aaacagttac caacgaatga atacaaatat agttacgaag
     2101 aattcatgaa gcgcaggaaa caattccaac aaagagttgt cacgcaacgg agtttgcgtc
     2161 aattaaatcg aacgattaat gcccatgaag aaaaatacca cgcaagtaaa gcctacgaag
     2221 agcaacaacg aagaattcaa atggaacaag aaagacagta aagacagtag gaggaatagc
     2281 aatgaaacaa tatttagatc aatggaaagt tatagaagga tcattaagag aagaacgaat
     2341 cgaacaactt cccgattgtt tagaaaaaga gcatttgttt cagataagag aaatgttgag
     2401 aaacgaacag tttgatccaa atcaattttt agtcgttgag tactcagcta ctggtgtgta
     2461 ctgttgtaat catgtgaagg gtgaaaaata ttttatcatt caagaatatg agggcaagtt
     2521 agcgccttat tatactactt gggaaatgaa tgaagagggg atcaataact tcccgtgtaa
     2581 aagcattgaa gagtctattt cactcacaga atgttagttg ttaagtgaat aaaagaagta
     2641 aaagagtcct gaacgtaatt gtccaggact ctttttttca atgaattgtt ttccgccaat
     2701 tcaatgagtg gtgcgatttg cgactacatt atatcaaaag aaagagaagg gaggaagaag
     2761 aaatggctta ttcaacgatt ggagttgttg ttgaaaaaag cagagacaat ctcgtttttg
     2821 tgacagaaat tcaaacgggc agagcttttg tcgtcacaga caaagcggca aaagcgtatc
     2881 aaagtggcga tattttaacg ttaaatctga ctacgaagac gttcgtcgat gcggcagaag
     2941 attatccatt
//
LOCUS       DAYLZO010000204         2909 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.213, whole genome shotgun sequence.
ACCESSION   DAYLZO010000204 DAYLZO010000000
VERSION     DAYLZO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.213"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..327
                     /locus_tag="ACGVJG_002993"
     CDS             <1..327
                     /locus_tag="ACGVJG_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HFJ6558878.1"
                     /translation="GVKKLGFKGYFVNSFLKPYSFMFPMKLIEEFTNLLTLALRLYGN
                     IYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSIQAFVFVTLSMVYMSH
                     KIEVEE"
     gene            380..595
                     /gene="atpE"
                     /locus_tag="ACGVJG_002994"
     CDS             380..595
                     /gene="atpE"
                     /locus_tag="ACGVJG_002994"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFJ6558879.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            686..1210
                     /gene="atpF"
                     /locus_tag="ACGVJG_002995"
     CDS             686..1210
                     /gene="atpF"
                     /locus_tag="ACGVJG_002995"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFJ6558880.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            1197..1739
                     /locus_tag="ACGVJG_002996"
     CDS             1197..1739
                     /locus_tag="ACGVJG_002996"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFJ6558881.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            1762..>2909
                     /gene="atpA"
                     /locus_tag="ACGVJG_002997"
     CDS             1762..>2909
                     /gene="atpA"
                     /locus_tag="ACGVJG_002997"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFJ6558882.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLR"
BASE COUNT          939 a          515 c          636 g          819 t
ORIGIN      
        1 ggggtgaaaa agttaggctt caaaggatat ttcgtaaatt cgttcttgaa accatatagt
       61 tttatgtttc cgatgaaact gattgaagaa tttacaaact tattaacact tgctttacgt
      121 ctttatggga atatttatgc gggggaagtt ttgctaacct tgattgcaaa aatggtaata
      181 agcttaggct ggttctcatt gccgttagca attccattag agatggtctg gatcgcattc
      241 tccttgttta tcggaagcat ccaagcattt gtcttcgtta ctttatctat ggtttatatg
      301 agccataaga ttgaagtgga agaataatat tcgttttaaa aacaaactac tatttatcta
      361 atttaggagg aaaacaatta tgaattatat cgcagcagca atcgcaatca tgggagcagc
      421 aatcggagcc ggttatggta acggtcaagt tatctctaaa acaatcgaat caatggctcg
      481 ccaacctgaa atgtctggtc aattccgtac aacaatgttt atcggggtag ctctagtcga
      541 agctgttcct atcttagggg tagttgtcgc cttgattcta gtttttggtg tttaattgaa
      601 tcagtattaa ggcagaaaca atttggtttg ttactgcttt tttacatgaa aactagaagt
      661 acttcaaaga aaggaaggct cgcctatgct gaatcaattg gcaattgcag aagttggcaa
      721 tgcgatgtta ggaaatgtca tcgttgtcag cggctcattt ttgatactac tggctctctt
      781 gaagcatttt gcttggggac caatcagcga tattttgaaa aaacgtgaag acaagatcgc
      841 caatgatttg gattctgcag aacaatctcg catcaactca gcgaaaatgg aacaagaacg
      901 cgaacaacaa ttgttagcct ctcgttctga tgcagctgat atcatcaaaa atgcgaaaga
      961 aagtggagaa ttaagccgcc aaaatatttt gaaggatgct caagaagaag cagctcgtct
     1021 aaaaagtaaa gcccaagctg atatcactgt agaacgtgat tcagcgctga actctgtaaa
     1081 agacgatgtt gcagaactat ctcttcaaat cgcggaaaaa atcttgaaca aagaattatc
     1141 tccagaaatg cacgaatcat taatcaatca atatatcgaa ggtctaggtt cttcaaatga
     1201 aactagataa atatacagta ggaagacgtt acgggaaagc tttatttgaa ctggcgatcg
     1261 attcaaataa agcagaagaa atttatcaag atctcgtaag cttacgtcaa atctatgaac
     1321 aagtacctgg attaggtaat atgttaagtg atgttcgtct agaacctcat gaaaaacgag
     1381 aaatcatgga caagcttgtt agtggctatg atggaatcgt taaaaacttt ttagaagttg
     1441 tttatgcgta taaccgaatg gatgatcttt tgttcatgat cgaagaatat gaacgtcgat
     1501 atgatgaaca tcaaggtttg ctgttgggaa gtgtcacaac agcagttcca ctttcagacg
     1561 aacaacgaat acaattagaa aagaatgttg caaaaacaat gaactatcaa tcagtcgaat
     1621 taaaacaaat cgttgattct tctattattg gtggcgcgat tgttgaagct aaccatcgag
     1681 taatcgacgg aagcattcgc acgcaattag aaaatatgcg taatcaatta aatagatagt
     1741 acaaagaggt aggtgaatcg aatggccatc aaagcagaag aaattagtgc cttgattaaa
     1801 gaacaaattg aaaattatca aaacgtgctt tcagtcgaag aaatcggtac cgtaacttat
     1861 gtaggtgacg ggatcgctcg tgcacatggt ttagaaaacg cgatgagtgg tgagttgctt
     1921 gaattttcaa atggctcata cggaatggcg caaaacttag aatcaaatga tgtaggtatc
     1981 attatccttg gcgatttcga gtccattcgt gaaggagata aagtaaaacg aacaggaaag
     2041 atcatggaag ttccagttgg agaggccttg attggacggg tagttaatcc gctaggtcag
     2101 ccaattgacg gactaggtga aatcgttact gacaaagctc gtccagtaga agcaatggca
     2161 ccaggcgtta tgcaacgtaa atctgttaac gaaccaatgc aaacaggtct aaaagcaatc
     2221 gacgctcttg taccaatcgg acgcggccaa cgtgaattag ttatcggtga ccgtaaaaca
     2281 gggaaaactt ccattgcgat cgatacgatc atcaaccaaa aaggtcaaga tatgatctgt
     2341 atctatgtag caatcggaca aaaagattct acagttcgta cacaagttga aacattgaaa
     2401 aaatatggcg caatggatta cacaatcgtt gtgaatgccg gtgcgtctca accagcgccg
     2461 ttgctttata tcgcaccata tgctggtact gcaatgggtg aagagttcat gtacaacggg
     2521 aaacatgtct tgatcatttt tgatgatctt tcaaaacaag ctgttgctta tcgtgaactt
     2581 tccttgctgc ttcgccgtcc accaggtcgt gaagcttatc caggggatgt tttctactta
     2641 cactcacgct tattggaacg tgcagcaaaa ttaagtgatg atttaggtgg cggttcaatg
     2701 actgccttac catttgttga aacacaagct ggagatatct ctgcttatat cccaacaaac
     2761 gttatttcga tcactgatgg acaaatcttc ttagaaagcg acttgttcta cgcaggtact
     2821 cgtccagcgg ttgatgccgg actatctgta tctcgtgtag ggggttctgc gcaaatcaaa
     2881 gcaatgaaaa aagttgctgg gactttacg
//
LOCUS       DAYLZO010000205         2903 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.214, whole genome shotgun sequence.
ACCESSION   DAYLZO010000205 DAYLZO010000000
VERSION     DAYLZO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.214"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1413
                     /locus_tag="ACGVJG_002998"
     CDS             <1..1413
                     /locus_tag="ACGVJG_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFJ6558883.1"
                     /translation="TTTAESSTSETMQKIEPKKDVYVIASDSAFAPFEFQNAKGEYEG
                     IDVKLMENIAKLQGFNIRMDYRGFSAALQALESGQADGMIAGMTVTDERKKSYDFSDS
                     YFDSGIQLAVKKGDTSIKSYEDLKGKTVGAKVGTESAEFLSDHESEYGYSIKYFDAAD
                     QLYDGLKVGAIDAMMDDYPVIGYAIKQGQELETPIKKESGGKYAFAVKKGQNPELLNM
                     FNEGLKELKRTGEYDKIIKEYVADGSETSSESSTADESTFLGLIQNNYQALLKGLWRT
                     ILLTLISFALAIVIGVIFGLFSVAPIRALRTIASIYVDIIRGIPMMVLAFFIFFGLPG
                     IVGFTIPDFLAGVITLTLNASAYIAEIVRGGINAVPVGQMEASRSLGLSYNRTMQKII
                     LPQAIRIMIPSFVNQFVISLKDTTIISAIGVVELLQTGKIIVARTTQSTYVYLIIAIM
                     YLILITALTKLAKVLEKKVK"
     gene            1415..2152
                     /locus_tag="ACGVJG_002999"
     CDS             1415..2152
                     /locus_tag="ACGVJG_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFJ6558884.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(2520..>2903)
                     /locus_tag="ACGVJG_003000"
     CDS             complement(2520..>2903)
                     /locus_tag="ACGVJG_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HFJ6558885.1"
                     /translation="FRVEESDIFDREGSEIYYELPLSFVQAALGDEVQVPTVHGDVKL
                     KIPAGTQTGTNFRLRGKGAPKLRGGATGDQHVKVKLITPKNLNEQQREALRAFAEAGG
                     IKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT          908 a          566 c          583 g          846 t
ORIGIN      
        1 actacaacgg cagagtcaag cacaagcgag acaatgcaaa aaatagagcc caaaaaagac
       61 gtatacgtaa ttgccagtga ctctgctttt gctccatttg aatttcaaaa cgcaaagggc
      121 gagtacgaag gcatcgatgt aaaattgatg gaaaacatcg ccaaactgca aggatttaac
      181 atccgaatgg actaccgtgg tttctctgct gctttgcaag ctttggaatc aggacaagca
      241 gacggaatga ttgccggaat gactgtaaca gatgaacgaa agaaaagcta tgatttttct
      301 gattcttact ttgatagcgg gatccaatta gctgtcaaaa aaggagatac ttcgatcaag
      361 tcttatgaag atttgaaagg aaaaacagtc ggagcaaaag ttggtaccga aagtgctgaa
      421 tttttaagcg accatgaatc tgaatatggc tattctatca aatattttga tgctgctgat
      481 caattgtacg acgggttaaa agttggcgca atcgatgcaa tgatggatga ttatccggta
      541 atcggttatg cgatcaaaca aggacaagaa ttggaaacac cgatcaaaaa agaatctggc
      601 ggaaaatatg catttgccgt taaaaaagga caaaatcctg agcttcttaa tatgttcaat
      661 gaaggattga aagaattaaa aagaacaggc gaatacgata agattattaa agaatatgta
      721 gcggatggtt cagaaacttc cagtgagtct tccactgctg atgaatcaac atttcttgga
      781 cttatccaaa acaactatca agcattgttg aaaggacttt ggagaacaat tctcctaact
      841 ttgatctcct ttgctttggc tatagtaatc ggtgtgattt ttggattatt cagtgtagca
      901 ccaatacgcg ctcttcgcac aattgctagt atttatgtcg atatcatccg aggaattcca
      961 atgatggttc ttgcgttctt tattttcttt gggttgcctg gtatcgttgg atttacgatt
     1021 cctgacttcc ttgctggtgt tatcaccttg actcttaacg caagtgctta tatcgcagag
     1081 attgttcgag gcgggatcaa cgccgttcct gtcggacaaa tggaagcttc cagaagttta
     1141 ggtctttctt ataatcggac aatgcaaaaa atcattttgc cgcaagcgat acgcatcatg
     1201 atcccatcat tcgtcaatca gttcgttatc tcattaaaag acacaacgat catttccgca
     1261 atcggtgtag tcgaattgct gcaaactggt aagatcattg ttgcacgtac aacacaaagt
     1321 acgtatgttt acttgatcat tgcgatcatg tacttgattt tgatcacagc attgacaaaa
     1381 ttagcaaaag ttttagaaaa gaaggtgaag taagatggca gaaaaaatct tagttgagca
     1441 tttagtcaaa aaatacggcg ataacacagt attaaacgac atcaatgtgt ccatcaacga
     1501 aggagatgtc gtctgtatca ttggcccttc aggttcagga aaaagtacat ttttgcgttg
     1561 cttgaatcgt ttagaagaac cttctgacgg tgaaattatt attgacggcg cccacttgat
     1621 ggataaaagc acagatatca acttggtgag acaacatatc ggaatggtat tccagcactt
     1681 taacctattc cctcatcttt ctattttgga aaacatcatt ttagcaccga tggacctgaa
     1741 gaaagaatct agagaagaag cagagaaaaa agcaatcaaa ttgctgcaga cagttggtct
     1801 agaagataaa aaagatatgt atcctgaaat gctttccggt ggacaaaagc agcgtgtggc
     1861 tattgctcgt gctctagcga tgaaccctga catcatgctt tttgatgagc ctacatctgc
     1921 actcgacccc gaaatggtcg gtgatgtctt gaacgtcatg aaaaagttag cagaacaagg
     1981 tatgacgatg gttatcgtta cgcacgaaat gggcttcgct aaagaagtgg ccaatcgtgt
     2041 catgttcatt gacgacggaa actttttaga agacggaacg cctcaacaaa tctttgaaaa
     2101 tccgcaaaat gaacggacaa aagatttctt gaataaagta ttaaatattt aataaaaacc
     2161 aaaaatgccc ggtcgcttca tgacgaccgg gcatttttag tttcttgttt tttcacttaa
     2221 ctgtaccagc cgcatcgctt gttctcctgc acaaacaagc aatcgctcta cctgttccat
     2281 cgcataagaa agtaccatcg gttcattgac gatatcaagt acatagtcga taccaccttc
     2341 agaaactatg atgattatct tattctaaag ctggcttttt tgttggtaca aacatgacaa
     2401 ctacaaaaaa accaggcgaa cgtatgattg ttcgtctggt ttttaattat taatgactta
     2461 gcgtttttac tagaaataga aaatttgggc aagtctactt tatattttta ctagaaaaac
     2521 tacttccgct ttttcccgcc aaaagcatct ttaaacttat cgaagaaacc gtcttcttgc
     2581 tgttcttcaa ctttgatgcc gccagcttca gcgaatgcac gtaatgcttc tctttgttgt
     2641 tcatttagat ttttcggtgt gatcagtttt actttgacat gttggtcgcc tgttgcacca
     2701 ccacgtaact tcggagcgcc ttttccacgt aaacggaagt tcgtacctgt ctgtgttcca
     2761 gcagggatct tcaatttcac atctccatgg acagttggta cttggacttc atcccccaaa
     2821 gctgcttgta caaaacttaa aggcaattca tagtaaattt cagaaccttc acgatcaaag
     2881 atatcacttt cttctacacg aaa
//
LOCUS       DAYLZO010000206         2878 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.215, whole genome shotgun sequence.
ACCESSION   DAYLZO010000206 DAYLZO010000000
VERSION     DAYLZO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.215"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..186
                     /locus_tag="ACGVJG_003001"
     CDS             <1..186
                     /locus_tag="ACGVJG_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFJ6558886.1"
                     /translation="KIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDVQTLTDNSI
                     KEVDKIAADKEKELLEV"
     gene            381..1193
                     /locus_tag="ACGVJG_003002"
     CDS             381..1193
                     /locus_tag="ACGVJG_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFJ6558887.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            1190..1990
                     /locus_tag="ACGVJG_003003"
     CDS             1190..1990
                     /locus_tag="ACGVJG_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFJ6558888.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            2151..>2878
                     /locus_tag="ACGVJG_003004"
     CDS             2151..>2878
                     /locus_tag="ACGVJG_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFJ6558889.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLT"
BASE COUNT          930 a          450 c          634 g          864 t
ORIGIN      
        1 aaaatcgcga ttcgtaacat ccgtcgtgat gcgatcgatg aatataaaaa acaacaaaaa
       61 aatggtgata ttaccgaaga tgatttgcgc ggattagaaa aagacgtaca aacattgaca
      121 gataacagca tcaaagaagt agataagatc gctgctgata aagaaaaaga attattggaa
      181 gtttaataaa atcataaaaa cagaggggcg gatcattccg cttcctctgt ttttatttat
      241 atttctttta tttaaaaatt aaaaaaagaa aaaattattc agtttttaaa taaaaaaaca
      301 atgatttcat aggaaaaaca gttagatatt gctataatac ttaagatagt gggaatcttt
      361 taagtatagg aggaaaaaga atgttacgtt tttttccgca aaaaaacaaa tatattcaag
      421 aaaaaagcga atttcaattt aataccgatg gaccaattcc tcagcacatt gccatcatca
      481 tggacggcaa tggtcgttgg gcgcaaaacc gtcgtttgcc acgagttgct ggccataaag
      541 aaggcatgga aacagtaaag aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt
      601 tgacgcttta tgccttttcg actgaaaact ggaagcgacc aaaagatgaa gtaagttttc
      661 tcatgcaact acctgtcgac ttctttgata cgttcgtacc agaattgatt aaagaaaatg
      721 tcaaagttca tgtcatgggt tatgaaaacg tattgccaga acatacgcaa gatgctgtaa
      781 gacgtgcaat tgagcaaaca aaaaacaata ctggaatggt attgaatttt gctttgaatt
      841 atggaagcag agcagagatc gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag
      901 gagaaataca cgcagaagaa atcgatgatg aattgattgc taagcattta atgacaggtt
      961 tcttgcctaa agagttacaa gatcctgagc tgatgatccg tacaagtgga gaagagcgaa
     1021 ttagtaattt tctgttatgg cagatcgcat atagtgagct gtattttaca aaagcgcttt
     1081 ggcctgattt tgatggtgcg catcttgaag aagcaatcgc ctcttatcaa aatcgagacc
     1141 gtcgattcgg cggtgtaaaa aaaacagaag atgagaaagg ggatcagtca tgaaacaacg
     1201 tgtggtcaca gcagccgtgg cgctagtctt gtttattccg attatatgga tcggaggcat
     1261 agcggttgag cttacagcag caatcttagc agtaatagga gtttacgagc tgtttcggat
     1321 gaaaggattg acactcttaa gttttgaagg agttttatcc gccgttggtg cagtgttact
     1381 tgttttgcca aaagaaagat ggtttttctt tcttccagag aatgtaagta catttatgtt
     1441 attttatctt atagttatga ttcttttagg aacatcagtt atttctaaaa atacgtatac
     1501 gattgatgaa gcaggttttc ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa
     1561 ttttgtcaat gcacgaacag aaagcttggc tgttttgata tttggtctat ttattgtttg
     1621 ggcaactgat atcggagcat atatggttgg tcgacgttat ggtcagcata agttaagtcc
     1681 tgatatttcg cccaacaaaa cgattgaagg tgcgctcggt ggtattggta gtgctgttgt
     1741 agtcgcattt ctttatttat tgctataccc agcaaaagaa ctatttaatc atggtatgtt
     1801 tgtcatgctt ctgatgacga ttattttttc agcagtcgga caatttggtg atttggtcga
     1861 atctgcaatc aaacgtcatt atgatgtgaa agactcggga aatatcttac caggccatgg
     1921 tggaatctta gaccgtttcg acagtttgct gtttgtattt cctatcatgc atttatttgg
     1981 cattatttag taagtatacc tgcgtttctt tgtgaatttc ataaaagacg caggttttta
     2041 tttttcatta tattgatact attggtgtct ttggtatact atggtaaaat tatagtgttt
     2101 tatcgttgtt ttataaaaaa cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga
     2161 ttctgacatt tatcatcgtt tttggtatat tagtgattgt tcatgagttt ggtcatttct
     2221 tctttgcgaa acgatcagga atcctcgttc gcgaatttgc tatcggtatg ggtccgaaaa
     2281 tttatgggca tcaagcaaaa gacgggacaa catatacgct tcgcctatta cctattggcg
     2341 gttatgttcg aatggcaggc aatggggatg atgaaacaga aatggcacca ggaatgcctc
     2401 tttctctttt actaaattct gacggcatcg tagagaagat aaatttaagt aagaaaatcc
     2461 aattgacaaa tgctatcccg atggaattga gccgttatga tttagaagat gagctgacta
     2521 tcactggtta tgtgaatgga gacgaaacag aagtagtaac ctaccctgtt gatcacgatg
     2581 cgacgattat tgaaaacgac ggaacagaaa tcagaattgc gccaaaagac gtacagttcc
     2641 aatcggctaa attgtggcag cgtatgctga ccaactttgc tggacccatg aataacttta
     2701 ttttggcaat cctgttattc atcattctgg catttatgca aggtggtgtt caagtgacga
     2761 acactaatcg cgtaggagag atcatgccta atggagctgc ggcagaagca ggcttaaaag
     2821 aaaatgatga ggtagtcagt gtggacggaa aagaaatcca ttcatggaat gatctgac
//
LOCUS       DAYLZO010000207         2838 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.216, whole genome shotgun sequence.
ACCESSION   DAYLZO010000207 DAYLZO010000000
VERSION     DAYLZO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.216"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            89..406
                     /locus_tag="ACGVJG_003005"
     CDS             89..406
                     /locus_tag="ACGVJG_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFJ6558890.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="ACGVJG_003006"
     CDS             complement(497..862)
                     /locus_tag="ACGVJG_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558891.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="ACGVJG_003007"
     CDS             1070..1552
                     /locus_tag="ACGVJG_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558892.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /locus_tag="ACGVJG_003008"
     CDS             1749..2639
                     /locus_tag="ACGVJG_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFJ6558893.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          980 a          439 c          540 g          879 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttt
//
LOCUS       DAYLZO010000208         2809 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.218, whole genome shotgun sequence.
ACCESSION   DAYLZO010000208 DAYLZO010000000
VERSION     DAYLZO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.218"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..374
                     /locus_tag="ACGVJG_003009"
     CDS             <1..374
                     /locus_tag="ACGVJG_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HFJ6558894.1"
                     /translation="PEGDILTHFDYGLRRFTFTAEEFAEHFEAQLLTIFKKVVERELA
                     MELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGSDAHVAEDYQLGFKKMSQLLADC
                     QVEKLLVIQGKERRLTPLPIL"
     gene            407..790
                     /locus_tag="ACGVJG_003010"
     CDS             407..790
                     /locus_tag="ACGVJG_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFJ6558895.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            866..997
                     /locus_tag="ACGVJG_003011"
     CDS             866..997
                     /locus_tag="ACGVJG_003011"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFJ6558896.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            966..1202
                     /locus_tag="ACGVJG_003012"
     CDS             966..1202
                     /locus_tag="ACGVJG_003012"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFJ6558897.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            1214..1300
                     /locus_tag="ACGVJG_003013"
                     /pseudo
     CDS             1214..1300
                     /locus_tag="ACGVJG_003013"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(1280..2251)
                     /locus_tag="ACGVJG_003014"
     CDS             complement(1280..2251)
                     /locus_tag="ACGVJG_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFJ6558898.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT          824 a          593 c          583 g          809 t
ORIGIN      
        1 ttccagaagg agatattttg actcactttg attatggatt acgccgcttc accttcacag
       61 cggaagaatt cgcggagcat tttgaggctc agctgctaac gattttcaaa aaggttgttg
      121 aacgagaatt ggcgatggag ttaaatgcca agagcttctt gaaatacggc aacgaagacc
      181 tttaccgtta tgccgttcct ttgtatcaaa gtgtcggcgg aaagctcttt accttaggct
      241 cggatgctca tgttgccgaa gattatcagc tgggctttaa aaaaatgagt cagctgttgg
      301 cagactgtca ggtagaaaag ttgctagtca ttcaaggaaa agaacgccgc ctgacgccgc
      361 tgccgatttt gtgattccta tataatagaa gaaagtggtg aaaaaaatga gcaacctgtg
      421 ggactacaat caagaggcac ccatccatta tttaattgcg cgacattggg atgcgttgaa
      481 aattgaggcg gtttgccgat ccttgttagc agccgttcca aaacaacaac tggaaaattt
      541 tttggtggct gacagcttgc agcgggaaaa agttcaggct tattttgcag cctttaaaga
      601 tcagcctttg gaatatttgc atgcgcagtt tcatcttttt tatcaagtag ccgccccaga
      661 tgactacaac gatttacgag gacagctgca attgaccttt caagcggatg aaaccgccta
      721 taccgtttta ttgggaatgg ctcgccttgg tgatcaggcg aaggtcgagt ggcggatttt
      781 cgatatttga tggcaggatc aaggcgtttc agttgcaaac cttcctctct ttcaattaga
      841 aaaaccgcag caggaagaag tttgtgtgct ggtcaccgat gctggtgaaa ttcgtctccg
      901 cttatttcct gaccaagctc ccctggctgt agccaactgg cgctccctag ctgagcaagg
      961 cgtttatgac aacacacctt tcggctcgtg tcattaagga atttgttatt caaggtggcg
     1021 ctttggatgg ttctggtgat gaagctgttt ccagctatga gggctttttc gctgatgaag
     1081 tgcatcgcgg actttatcat tttaatggcg ccttggcctt aggggaccac ggcccccaca
     1141 ccaacggcaa ccagtttttt attgtgcaaa acacaaaggc gcaggctgac ctgctaatgt
     1201 aaagcaagca tacttggagc ttggaggact accggagctg gatggtcgtt atactgtttt
     1261 tggacaggta taccaagggt tagaaatcgt agcagcgatc gcagctcagc cgacaaacag
     1321 tgaagatgta ccgattgagc cagtgttcat tcgacaaatt aacttcgaaa aaacatcgta
     1381 aaaagacact cactgacttc agcaaaagct ctattcttga gctggctgca gctagtgagt
     1441 gtctttcatt tatccataag ataaagcttg gattcaataa ataaataaat gttttggtta
     1501 aagctgtgac cagcaaatga ggaaccgtgc gagccagttg atctgccaat gcaaacattt
     1561 ctttaggtga aggcgctggc gtgtctgcca acggatactc cagtcccaat gcttggtaac
     1621 cgtgcatacc atagcagtgg tacggtagta attctaacgg tacgaaccga tcaagatctg
     1681 ccacaaaaca agcaatcgtc agcagctcgg tcaggttcat attgatacct ggaaccacag
     1741 gaactcgaat tcgcacttcg cagttaggat actcaattaa aaagcgacga atattcttca
     1801 aaatttgttg gttatctaag ccggtccatt gtttgtggag cttgctggta aacattttta
     1861 catccacaaa caatgtatcc acatagggag ctaccttttg gatttcatta taagcaccac
     1921 aaaagctggt ttccagtacg gtattgattc caatgtattt cgatttttgc aaaatttctt
     1981 tagcaaaatc cgcctgaacc agtgcctcgc ctccgctaat ggtcactccg ccatccgagt
     2041 gaaagtaaaa aacagcatcc ttctcaatct catccattac ctcttcagcg gtcatggtct
     2101 ggccaaatcc tttttcaatc tgcttgcttt gtgattcggg aatcgaacac caggcacagc
     2161 gtaatgggca gccctttaaa taaacaacgg tccgtaaacc agcaccatca tgtagagaac
     2221 cctgttctat tcttaatact gttccttcca tatagatttt tgaattacac actagtttaa
     2281 actaaaacac acaaggaaaa acttaccatt taatcataaa attagctcat ataaatatac
     2341 taggatgtaa aatagactgg tcaaaatctg gtcaaaaaag aggactaata caggtagtcc
     2401 tcttttttgt tattttaata ttctaaatca ttttttattg gtttattccc ttattcgatg
     2461 tagactcaaa tcactatatc actagatatc aaacggcgtc atacggaatc aaaaacatca
     2521 aatttgacct gttaaatggt cattgaataa cattcaaaat caataaaacg gagtcagaca
     2581 cggagtcaaa aacgctgaga gtaaaaaata tatacagcct tgtaggtaaa gtaccacagg
     2641 gctgtatatt ttttatttca gattacctta aatgattcca tttacttttt atctcatttt
     2701 ttcctactag ttagtatcaa atactctcct attttctcaa taaactattt ttggatacat
     2761 ggtatctgaa acagctaaaa ccttgtcatt acagcaaaaa atcacgtta
//
LOCUS       DAYLZO010000209         2804 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.219, whole genome shotgun sequence.
ACCESSION   DAYLZO010000209 DAYLZO010000000
VERSION     DAYLZO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.219"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..1148)
                     /gene="ltrA"
                     /locus_tag="ACGVJG_003015"
     CDS             complement(<1..1148)
                     /gene="ltrA"
                     /locus_tag="ACGVJG_003015"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFJ6558899.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
     gene            complement(1894..2031)
                     /locus_tag="ACGVJG_003016"
     CDS             complement(1894..2031)
                     /locus_tag="ACGVJG_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFJ6558900.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(2028..>2804)
                     /locus_tag="ACGVJG_003017"
     CDS             complement(2028..>2804)
                     /locus_tag="ACGVJG_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFJ6558901.1"
                     /translation="GCRQFETFLLAQKRSWYDFFEDCLKAGGVMKRLDLAINDRVGLL
                     DIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGMGNTLYIGSLKSEVYFCLYEK
                     DYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLLKGRSIESTTFSIINRYLRFA
                     DKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYTIERTLNWLGRQVAPTWKMAK
                     ELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENLII"
BASE COUNT          716 a          621 c          435 g         1032 t
ORIGIN      
        1 tctttttggt ttttgaattg tttatcactt atatgtgatt taacaactaa cttctttctt
       61 ctcggtatca ttttaatctt gaaaccaaga aagtcggagt atctttttct aacatttaca
      121 attcttgttt tttcttgtga tacttccaat tttaatcgtt gttcaatcca ttgagtgatt
      181 gctattttgg ctttttcagc actttccttg tagcggcaga aaactctaaa atcatctgca
      241 taacgaacca tatacatttc ctttaacttt gttttcttca tttctttgta agcgttgctt
      301 ttacatgggc taccgctttt attgatatga accacatagt tttttgtaac aggattttcc
      361 tgccattggc tttctaccca atggtctaac tcattaagaa caatgtttgc tagtagtggg
      421 gatattattc ccccttgcgg agttcctttc tctggatatg ttattgtacc gttagtcatt
      481 ttgattggag cttttaatat cctttttaaa atgaatatca agtgcttgtc ccgtattccc
      541 atagcccata tctgttttat cagcttggaa tggttcacgt tgtcaaagaa acccttaata
      601 tcaaattcaa tcacataatg tagctggctg atttgtaggc ggttataggt tgcttttatt
      661 gcattttcaa ctgacctatt aggtctaaaa ccatagctgt tttcactaaa tttagcttcg
      721 cagattggtt ctaatacttg ttttatacat tgttgtataa gcctgtccca catacaagga
      781 atacctagag gtctggtttt accatttggt tttggtattt cttttcgcct cactggctta
      841 ggtcgatacc catgtggact gcctgcaaca atgtatctta cttttttgac aactgtttct
      901 gctggcaatt tgccaatatc tttaattatt gtaccgtctg ttcctgctgt tttactgcct
      961 gcatttgctt tgatatttcg atatgccagt aatatatttt ctctcgatag gataatatcc
     1021 ataagatgag tgaaaatatc tccattctta cttttggcat ataaatcatc aaaggtgttt
     1081 tgcatttcgt aatattcagc gtgtcttaaa tcttctacac acaatgtttc atttttattt
     1141 ttaggcataa ggcttcactc ctttacttga aattgagtga gcttttttat cttactcgta
     1201 atccttattg aaacaacata atacttttct aatatataca cgacttgagg gcattcctcc
     1261 attctcatta caagaatttc ttaggttcgc cctctgcttt tcagcattag ttcaactgct
     1321 tattgttctt cgtcagttta ttctcttgat gagaacccaa tacctttcct cgttccgata
     1381 atcttatcta tacatatata tccttaggtc actagctata agcctgctgg cttgataccg
     1441 cctgtaacgt tatatggcat ttcataacct atattcttac tttgccacac cagctacgat
     1501 ttctcgtacc tttgcatttc tacaaagttc aaatttagac ccgtacattc gctgtttcgt
     1561 cagttcctta tagaacattc taaccataga tattttatag acccccgacc tgttggatac
     1621 gctacccacc tctggatagt tttcgataga agatatatgt tcatcaacta ttacggtatc
     1681 tttcagccgt ctttaccgag cttcaaacat tctgagtttc ctcatacaat gcttgtcgga
     1741 ggctcagggc aggcgtttca gagcgttact tcatcattcc acctgtcaga ttatcagttc
     1801 tcctaataac caaacttttt cgttctttta ttagtgcctt agcttttcac taaggaacga
     1861 gtcgcacata aattttgtcc gaaattcatc ttgtcacttt cctttctttt ccattgctcg
     1921 atccgctgct gcacttgcat tcatcaagca catcacagta acaccaagca tactgccacc
     1981 aaataccaaa aaaatatctt ttacaattcc catgacgatt cctcttttca tataatcaaa
     2041 ttttcaattg ccatactttg ttgctccaaa atctttttat gtcggtctga taagcgtgca
     2101 ttctgtacca tatcctggat ataggttgtc cgattcaatc gatccaactc cttagccatt
     2161 ttccacgtag gggcgacttg tcggcctagc caattcaagg ttcgttcaat ggtatacggt
     2221 tttggctcgg aagttaattg aatctctttc cgattccgtc caataaaacg cccccattgt
     2281 tcattcaagg gccagttggt tcgcctttta ccttctactt tatccgcaaa tcgcaagtaa
     2341 cgattgataa tggaaaacgt ggtgctctca atgctacgac cttttaacaa atcttgaatc
     2401 gcatgaaaag cacggtcatt tttcagtcga atctcaaaac gatttttcgt ctcagtttgg
     2461 tctaatggaa tccctaactt gatatattgt tcatagtctt tttcatacaa acagaaataa
     2521 acctcactct tgagactgcc gatatataag gtattgccca tgccgtcctt ttcatcagcc
     2581 tttaaaagtt ccccggaacg ataactttta aaggtacgaa acaaggagat acattcttcc
     2641 ttctgacatt ttttcgttag ctcaggtata tccaatagcc ccactcgatc attgattgcc
     2701 aagtccaagc gtttcatcac gccaccggcc tttagacaat cctcgaaaaa gtcgtaccag
     2761 ctacgctttt gagcgagtaa aaaagtttca aattgtcggc aacc
//
LOCUS       DAYLZO010000210         2789 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.220, whole genome shotgun sequence.
ACCESSION   DAYLZO010000210 DAYLZO010000000
VERSION     DAYLZO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.220"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            133..>2789
                     /locus_tag="ACGVJG_003018"
     CDS             133..>2789
                     /locus_tag="ACGVJG_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFJ6558902.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDR"
BASE COUNT          884 a          535 c          602 g          768 t
ORIGIN      
        1 aaaataaacg cccagacatt tgcgtgtctg ggcgtttttg tctattttct ttaaaaagcg
       61 atgagagtat ttttctgcac tttgtagtat aatttcaaaa gaacattaga ggcaggagaa
      121 gaggaagata ttttgaaacc aagaacttat gcagtcgttg atatcgaaac gactgggacg
      181 aatccaaaag aagatcggat catccaattt ggctgcgtca tgattgaatc aggtcggatc
      241 acttcacgtt tttcgatcga tattcatcca gggcggaaga tttcaaaaca aatccagcat
      301 ttaacaggga tcacgaatca acgcgtccaa aaagcgcctt atttcgaaga tgtcgcccaa
      361 acgatttaca atcttttggc agacaccata tttgtcgcgc ataatattta ttttgattat
      421 aattttttga accatgaact catgcgatgc ggacttccta gtttgaagat accaggcatc
      481 gatacagtag aacttgcaca aattttcttg ccgacggaac caagctttcg cttagctgat
      541 ttgtcagaaa gccttggatt tattcatgag aatcctcatc aggcagatag tgatgccgaa
      601 gtgacaggtc aacttctttt gctgatcgaa gaacggatga gaaaattgcc aatcatcacg
      661 ttagagcaga ttgcgcgatt aagtagacac acaggtatgg atacgagtcg tttcatttat
      721 catgtgattg aagaaatgaa agaaaaacca gagccacttg atccctcatt agaaatcgtc
      781 gatgggctag ctttgcagaa aaaagaagtg gaactattca catccgtcca ttatggcgag
      841 cggacatatc caagaaaaaa acaagcaaaa gaaaaaatgt ttggtaaaac actgatgtat
      901 cgaaaagaac agaacagact gatgaatgcg gtctacgacc atttcacaaa agatgaatca
      961 aaagatttga tgatcgaagc agctacagga atgggtaaaa caatcggtta tctgctgcca
     1021 ttgagttatt tggctacacc tgaaaaaccc gctgtcatca gtaccgtttc gctagttttg
     1081 cagcagcaga tcatagaaaa agacattcca ttgctgaatc aattgctaga tcagcccata
     1141 caacctgtaa ttatcaaaag ctatcgccac tatattgatt tgcagcgttt taaaggcaca
     1201 cttgtggaac cgccagaaca aaaacaatat gctttgtatc aaatggcggt cttagtttgg
     1261 ctgacgcaaa cgaagactgg tgatttggat gaattacatt taacaaattt gaaccattca
     1321 ttctttgcag atatcgcgca cagaggtatt ggatttttag caagaaatca gtcattttac
     1381 gaacaagact ttgttcgttt cttgcaaaag aaaatcaggc aaagtaattt tttgattgtc
     1441 aatcatgcgt ttttagtaca agaaacacag cgaaaagaac cattgcttcc agctagtcca
     1501 tttttgttga tcgatgaagt acatcatttg ccggaaatcg ctgaaaaagt aaatgacaaa
     1561 cgattaagta ttaactattt ccataaacaa atccagcaat tagaagaacc aaatcagctg
     1621 tttgaccgaa tacaaagcta tttaccgaaa gaacatgaag ccttacgctg gttatcgatt
     1681 tatcgagaag aactttctgc gctatgcgaa gcacagattt gtatggcaga aggattaatg
     1741 agtttagtga gcggcaagca acctaacgga cagtatccag aagaagtcat gatgacaaaa
     1801 gagatgttgg atcagctgcc actggaatct gggacagcca ttgatcacct gcagcttttt
     1861 tatcaggaga tccagcagct acagtcacaa atgcgtctga cttttgagat tatccaagaa
     1921 agctggacaa accagatgcg gatcgattta gcaagtttat atagcctatt tgatcaatta
     1981 gacaagcaat acttgctgat ggatcaatgg ctggaagact ggcgagatca tttgatccat
     2041 tggttcgttc ctgggaacac agcgcagcag atgatttttc atctccatga ttttcaagcg
     2101 gctatgttgc cgtcgactgt ttggtatcct cgatatgatc gtattcttta tattggagga
     2161 acgctgaaga tcggccctaa tcgacactat ctctctaaga ggcttggcat tgaagcagca
     2221 cctcttaaag tgattgcctc tccttatgat tatgagaaac aggcaaggat ctatgtgcca
     2281 aaagaggcac ctgccgtcca tcaagtcagc aatcaagcat atatcgatta tttagagaca
     2341 gcacttacag gtctgatcga acaagaagat cggccaatac ttgtgttatt cacttctcat
     2401 gatgttttgc agaaggtcta tcagaaaatg catctgtcta tgctagaaaa aggacgagaa
     2461 atactagctc aagggtttgg tggaagccgg gagaagcttt tgaaacgctt cattttgtca
     2521 gaaaaaagcg tattgtttgg cgctgacagc ttttgggaag gtgtagattt gcctggagat
     2581 gctttgcaga tcgttgtagt cacacgttta cccttcgata atccgcttcg cccgatggtc
     2641 aaagctagga acgcttattt agaatcagag gggatcaatc ctttttacca ggaatccgta
     2701 cctaaagcag ctttgaagtt acgccaagca ttaggtcgct tgataaggac ggagacagat
     2761 cgtggcgtat tgcttatttt ggatcgaag
//
LOCUS       DAYLZO010000211         2779 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.221, whole genome shotgun sequence.
ACCESSION   DAYLZO010000211 DAYLZO010000000
VERSION     DAYLZO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.221"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            1..788
                     /locus_tag="ACGVJG_003019"
                     /pseudo
     CDS             1..788
                     /locus_tag="ACGVJG_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2301..>2779
                     /locus_tag="ACGVJG_003020"
     CDS             2301..>2779
                     /locus_tag="ACGVJG_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFJ6558903.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMRGKRKPNGHQEKRGKQQYQRSIHDRSDNYPDFNSE"
BASE COUNT         1012 a          390 c          554 g          823 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaacg
      721 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
      781 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      841 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      901 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      961 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
     1021 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
     1081 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
     1141 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
     1201 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
     1261 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
     1321 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
     1381 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
     1441 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
     1501 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
     1561 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
     1621 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
     1681 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1741 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1801 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1861 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1921 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1981 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     2041 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     2101 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     2161 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     2221 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     2281 caaaggagtg taacccataa atgacctaca aacatcttac catagacgaa ctgacaatga
     2341 tagaatcata ttatcttcaa cataataagc cggttgaaat cgctaaccga atgggacgtg
     2401 ctatacaaac tatttataat gtagtcaata agttcaagca aggcaagact gctcttgatt
     2461 attggcacca gtataaagaa aataagaaaa aatgtggtag aaaagtcatt caattacctg
     2521 ctcatgaagt agattacatt aaagagaaag tcactcttgg ttggacgcct gacgtcatta
     2581 tcgggcgaaa agaaaggcct gtttcatgcg gtatgagaac actttatcgt ttattttcta
     2641 aaggaatatt tgatattgac acactaccga tgagaggtaa aagaaaaccc aatggccatc
     2701 aggaaaaacg gggaaaacaa cagtatcagc gctcaatcca tgatcgatct gataattatc
     2761 ctgatttcaa ttctgagtt
//
LOCUS       DAYLZO010000212         2777 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.222, whole genome shotgun sequence.
ACCESSION   DAYLZO010000212 DAYLZO010000000
VERSION     DAYLZO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.222"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..414
                     /locus_tag="ACGVJG_003021"
     CDS             <1..414
                     /locus_tag="ACGVJG_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6558904.1"
                     /translation="SIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSSGRIVYDD
                     LEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEPSWKNLKA
                     VTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(491..1009)
                     /locus_tag="ACGVJG_003022"
     CDS             complement(491..1009)
                     /locus_tag="ACGVJG_003022"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFJ6558905.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(1097..1246)
                     /gene="rpmG"
                     /locus_tag="ACGVJG_003023"
     CDS             complement(1097..1246)
                     /gene="rpmG"
                     /locus_tag="ACGVJG_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFJ6558906.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            1474..1920
                     /locus_tag="ACGVJG_003024"
     CDS             1474..1920
                     /locus_tag="ACGVJG_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFJ6558907.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(2115..>2777)
                     /locus_tag="ACGVJG_003025"
     CDS             complement(2115..>2777)
                     /locus_tag="ACGVJG_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HFJ6558908.1"
                     /translation="YESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDEIMTVH
                     YEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMKLAQQI
                     PSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVKALFEA
                     DFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAEAIDYV
                     TV"
BASE COUNT          882 a          522 c          494 g          879 t
ORIGIN      
        1 tccatcaaga aaactcgaga agaactagca gataaaatcg agaacaagac agctgcagtg
       61 ttgtgggtga ccaacaactc agcctttatg gtcagcaaaa atcggtcaag tggaagaatc
      121 gtatacgacg atctggaatt tggtgttcct gatctagtgg aagaggtctc caaaaaagcc
      181 acttctgact ggtctgctgt ttcctctgaa aagctagctg aattagatgc tgactacatc
      241 atccttgtga acagtgacaa agatgctgcc atgttcaatg aaccaagctg gaagaatcta
      301 aaagcagtga cagacaatca tgtactgcaa ttcggacctg aatctagttg gctatataac
      361 ggacctattg ccagtcagga aatggtcaat gatatcaaag aaaaactaaa ttgattttaa
      421 ctaaaaaaag catcttcacg attagtcgtg agatgctttt tttcttatta acaaactaga
      481 cgcatagatt ttattcttct tttgaagcaa ccctattttt tactctttcc catttttgaa
      541 aacgatgggc atatagattt tcttgatccg ttatcccctc atactcctct ataagggcga
      601 agtctgaaaa atcaatattc ccaatatacg tatcgccttc aaacgtgcta tcgatcaatg
      661 ttcgccagat ccgaccagtg taaggaagaa gttcttgaaa aatccgacta ccacccgaaa
      721 catagatatc ttctgagatc gtttgactat acgacaaaag ttcatcgatc gaatgcatta
      781 cttccgcatt ttctttcgtg cgaaaatttg gttgcgttgt caacacgatg atatggcgat
      841 aaggaagtga aagatccccc attccttcat aggtctttct tcccatcact aaaattcgat
      901 ccattgtatg ttcgcgaaag aaacgcatat catttggcaa tcgccatggt aacaaaccat
      961 ttttgccgat cagaccattt ttatcttgtg cccacatgga aataaacata ttatccgctc
     1021 cttatgattt tagatgtttt gagataaaaa aagtctcaat tctttgacca gcaaggaatt
     1081 gagaacttta aaaacattat tttgtttcac ggtgcaaagt aactttgcgt tcgcgtgggc
     1141 aatatttttg tttttccaaa cgatcaggat tgttacgttt gtttttgctt gttagatagt
     1201 tacgttcttt acaagaagta cactctaaag tgatgtttac gcgcatgttt ttccctccca
     1261 tttttacaag aatcctctaa gatgactctc agacttgatt atcttaccat gattttttct
     1321 agattgctag tgtttttcac gattttgtta agtaaaacta cttgacgata aatacaacgg
     1381 ttttacttct caaacaaaat ctttttcttt ttgtataaat acccttacac ttgtttatat
     1441 agattatgtt ttttaataaa gggggatact aatatgccat ggaacatgaa ggactttcct
     1501 gcatcaatga aaaacttaga taaattgaca agaaaaaaag caatcgacat cgcaaatgca
     1561 ttattagatg aagggtaccc tgataaccgt gcgatcccaa ttgccattga tcaagcaaaa
     1621 aaatggtatg aagatgctag tgaatcagaa aaacgaactt tcgaaaaaga aaaaaatcca
     1681 agcaagacgg atgaacacga tacgaatcct cgtgctggaa aactgcttga ttcagatgtg
     1741 atcgtagaat atgaggaaga acaatggatc gtgaaatcta aaggggcaaa aaaggcaagc
     1801 aaccattttg atacaaaaaa agaagcaatc gaaaaaggaa agcaagtagc acaaaacaaa
     1861 gaatcaactt tgttcattta taaaaaagat gggacaaaag aaaaagaaat cgattattga
     1921 acatatgtaa aggggttgtg acgaaaatcg tgtcacaacc cccttatgtt cgaaacaatg
     1981 gtgttcctct gccaactccg aaccataagt cataatatct ataaaacggg aagagctgtt
     2041 ttttgaaatt gtttcttatc actcagtgtt aaacggtagg agaaaacctc ttttttattg
     2101 atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg aatcgtttca
     2161 tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat ttttctttgc
     2221 tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc ttgatcaaaa
     2281 aattcttttg caaaatctgc ttcgaataat gctttgactt gttcgtagcc gtgttcttct
     2341 ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga ccattcttcc
     2401 ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg attcaataaa
     2461 aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa aggtactcgg
     2521 acttccaaag aacttgccat agaaagttta tctgccttta acaagatatc ttttggcatc
     2581 cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc ttctgttttc
     2641 tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt tggtgttaac
     2701 acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata ttcttttgca
     2761 ggagcaagtg attcata
//
LOCUS       DAYLZO010000213         2699 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.225, whole genome shotgun sequence.
ACCESSION   DAYLZO010000213 DAYLZO010000000
VERSION     DAYLZO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.225"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            419..914
                     /gene="dfrF"
                     /locus_tag="ACGVJG_003026"
                     /pseudo
     CDS             419..914
                     /gene="dfrF"
                     /locus_tag="ACGVJG_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
     gene            1019..1675
                     /locus_tag="ACGVJG_003027"
     CDS             1019..1675
                     /locus_tag="ACGVJG_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFJ6558909.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            1857..2387
                     /locus_tag="ACGVJG_003028"
     CDS             1857..2387
                     /locus_tag="ACGVJG_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFJ6558910.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT          982 a          352 c          558 g          807 t
ORIGIN      
        1 gtttcccaaa caaaaagata ttaagaaaca gcccatgttt acctgcttgc aaacaaaaga
       61 atattgctac tagactaaga gatactacat aagtgtgttg gtatctcttt tttgttgccg
      121 tgaaacagca gactatttca tttctgcttg attaccttaa aacttattag tctatggttt
      181 gtcaagggct tgttgcgtga gaaatgctga atatcaccct ttacaaaaca taggctgata
      241 agtaactttt caaaagcaga aaagaaatgg caatgcttat aacaatatga tataatgcag
      301 atgaagacaa aaaaggaatt tgtcgagctg gttagttcga gataattttg agtttgttaa
      361 gatgtggtgc acttgatgga atcacaggta cgacatcata ttatggagga tgaaatagat
      421 gataggtttg attgttgcga ggtcaaagaa taatgttata ggcaagaatg gtaatatacc
      481 atggaaaata aagggagaac aaaagcaatt tagagagtta acaacgggta atgtggttat
      541 tatggggcga aagtcttatg aagaaatcgg tcatccgttg cctaatagaa tgaatattgt
      601 tgtttccacc acaacagagt atcaaggaga taatttagtt tcagttaaat cattagaaga
      661 tgcattatta ttggctaaag gacgagatgt atacatatct ggtggatatg gactatttaa
      721 ggaagctttg caaatagtag ataaaatgta tatcacagaa gtagatttaa atattgaaga
      781 tggagataca ttctttccag aatttgatat caatgatttt gaagttttga taggggaaac
      841 acttggtgag gaagtgaaat atacgagaac attttatgta aggaaaaaat gaattgagta
      901 aattttggat ttagggtgtt ttcataaata tattgctttt tatgcatata taaattgttg
      961 taagacaaat tccagtttgt atggggcgat aaaatcagaa tttgagagag gattgaatat
     1021 gaataaggaa tggtctgaac ttaataaaac aatgcaggca caaataaaaa agaaagatac
     1081 ttataagagg ggtattgata ctttacttac tttacgaagt cagttaatac aaaccttagt
     1141 atctttcaaa gaagaattat gcagagaaga ttttaactca atccccttca taaacgctga
     1201 tggttatcat agtaagacga ttgcttattc tatttggcat attttccgca ttgaagatat
     1261 agttgtgcat acagtgataa atgaagatga acaagtattt tttgcaggca attatcaaga
     1321 gcgtatcaat tcacctatca ttacaacagg aaatgaactc atgaaacagc agattgcaga
     1381 cttttcaaaa cagcttaatc tggaagaatt atatttatat attttcgaag tatgggaaag
     1441 cactgagaaa atgcttgaac ggttatctta tgatgaattg aaaagaaaaa taccgaaaga
     1501 aagaaaagga tacttagaat cgttgaatgt agtaaacgac aatgaaaaag caatttggct
     1561 gattgattat tggtgtaata aagatatttg tggactaatt caaatgccgt tttcaagaca
     1621 ttggattatg cacacagaag cctgtttgcg tataaagaac aagatacatt cataggcaaa
     1681 aatttcagtt tgtagaacta aggaaattgg aatttaagga ggaataaagt atggttagtg
     1741 attttataag agcagcattt ccatggattt taatgggttt atttgtagca atcagttgtt
     1801 cccttatgag taaaaaagag aaataaattc ctgtttgtat ttataaaaga gaaaaaatga
     1861 gaaaagtagt tttatttatt gccatgagcc ttgacggata tattgcggac ggtaatggtg
     1921 gagtagcttg gctaaatggt catggaaatg acaatgaaaa catcgacact tatacagaat
     1981 ttaccaaaga tatagatact gtcttaatgg ggtggaatac ttctcatcag gttgttactg
     2041 aactttctcc gcaagaatgg gtatataata aatttacaac ttatgtatta acacataaag
     2101 agtgcaattc ttcccaacaa attcatttta caagtgaaaa tcctgtttta ttattggaac
     2161 ggctaaaaca agaagcagga aaggatattt ggatttgtgg cggagcaaat cttgttcagc
     2221 agttggtaag caaaaatatg attgataaat attacatttc tgttattcct actctgttag
     2281 gaaatggggt tcgtcttttg ggcaacatag acagggaaat aaagttaagg ctttgtaaaa
     2341 cacagactta taacggaata accgatttga tttatatgcg tagataacta gagagaaaca
     2401 gaaaagctgt taaaccgacg gagcgatccg accacgttgg cgggaaaatc cttaagattt
     2461 ttccgccatg cttgcaaggg ggcggttcgc ttcggcggtt cggcgagctt gcgagccgtt
     2521 caaagccccc gttatctaac aaggggggca caaaaaacaa gaaacactat acactaacaa
     2581 gcggaaaacg ctgtatttat aaggcaaaac cccattttta cagtagtgat ataaattgta
     2641 cctatatcac tatgaaaatt gaatagtaaa tatgagaaag acagttgatt tcaaaaaat
//
LOCUS       DAYLZO010000214         2678 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.226, whole genome shotgun sequence.
ACCESSION   DAYLZO010000214 DAYLZO010000000
VERSION     DAYLZO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.226"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..155)
                     /locus_tag="ACGVJG_003029"
     CDS             complement(<1..155)
                     /locus_tag="ACGVJG_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFJ6558911.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADIT"
     gene            complement(155..997)
                     /locus_tag="ACGVJG_003030"
     CDS             complement(155..997)
                     /locus_tag="ACGVJG_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFJ6558912.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            1319..2506
                     /locus_tag="ACGVJG_003031"
     CDS             1319..2506
                     /locus_tag="ACGVJG_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFJ6558913.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          876 a          502 c          449 g          851 t
ORIGIN      
        1 gtaatatctg ctaaaatcga attagtaact actaccttta atttttcgtt cgaagccact
       61 gtatctttgt ctttactatt tgttgaacat gccccgaata taaaaagagc aatcaaaatc
      121 cccagacttc ccagtactgc tttccatttt ctcatcataa ctttctctcc ttctttctta
      181 aaaatttttg ttttggtgag attagaaaac taattacaaa taagaatgcc gctgttaaaa
      241 caatacttga tcctgctgct atgttaaaac tataaccaat gaacaaacct agcaatgatg
      301 agcacgctcc gactgtcgcc gaaataaata tcatagtttt taagctgttt gcatataaat
      361 atgctgtagc agctggtgta attaacattg caacaattaa tatagttccc acactttgca
      421 tagctgttac tgctactaaa gttaatagaa tcattaatag ataatgataa aaactgacat
      481 tcatccccat tgcttttgcc attaatggat caaaagaagt gattaataat tctttaaaaa
      541 atagaacaac tgtaaccaac actaaaacag aaacgccaat tgtaatccac ttatcaatat
      601 cttgtacagc taaaatattt ccaaataaga tatgaaataa atccgttgag ctatttgcaa
      661 ctcctataag gataactcct aatgctaaaa aagaactgaa agtaatacca attgctgtat
      721 ctccttttat tacactattg ctttttataa aagtaataat gatagaagct aatagaccaa
      781 atacaatcgc accaataaaa aaattaatac ctaaaatata agaaattgct actcctggca
      841 aaacagcatg agaaatagca tcacccataa gagacattcc tcttaaaata ataaaacaac
      901 ctactgttcc agaaacaatc cctataacta cagatgtaat tagagcattc tgtaaaaagt
      961 gaaagtcata cagaccatta ataaaatttt gaagcatatt attccccctt gtatattaga
     1021 gttagggttt taaccttccg gttttgattg gataacccta tgaaccatcg ttattttaat
     1081 ttggtattaa ctcatttctt ttgagataat atatttattg gattgagagg ctaagccgtt
     1141 ccccccttgg gccattgaac ccgcactcga gagactggct cctctctctg ataagtggat
     1201 tgcgtatgag ttagatgttg aaagtcctta ctttactccg tatctcgtac aaggtagtga
     1261 cgcttttcag ttgagtggat ttcaatccaa aattcttttt ttgaaaggtc agatatacat
     1321 gtacttttta ggcattgata tcggtaaacg cactcacgtt gcttctatca tgaacgagga
     1381 aggaaaggta ttactaaaag gtttttcatt tcctaacacc actgaaggag cagagtcatt
     1441 aattgagcga atggtcgact attccggagc acccagtgac tttgttatag ggatggaagc
     1501 cacggggcac tattggctgt ccatcttttc ataccttcac gaatcagatt atttgattca
     1561 cgttgtgaat ccccttcaaa cagacggatg gcgtaaaggt acagaaatac ggaaacgaaa
     1621 aaacgatatt attgattctg ttttgattgc tgacttaatg cgctatggct catttgtcga
     1681 aacggtctta tccgatgaaa atgttttttc tttgaaacaa ctttctcgtt accgaactta
     1741 tttagtaggt acagcgagcg acttcaaaag aaaaattata gccgtattgg atcaagtctt
     1801 tccagaatac gcgacgattt ttaccaagca gggcgttttc ggaaaagctt ctaaagagtt
     1861 attgctggcg tttagtgccc cgatcgactt agaaaacatt tcctcagaaa ctttggccca
     1921 atacttagct gagttaagca ggaaccgaat caaggcagaa aaagcggacc aattgaagca
     1981 agcagcttct aactcctttg gagtaaaatt tgctcaagaa gcgttcacct tccaactacg
     2041 ttcaatgatt gaacaattaa aattcattga aagccaaata aaggaaacgg aaagagaaat
     2101 caagcacata atggacactt taaattctgt tattctcaca attcctggaa ttggtcctat
     2161 aaacggtgcg actattcttg gtgagatcgg agatattcaa aaattttcta acccgaaaaa
     2221 actcgttgct tacgctggga tagacgcttc cgtgacacaa tcagggcaat atgaggcaac
     2281 gcataatgtt atgagtaaac gagggtctcc atatttgcga aaagccttgt tttcggcggc
     2341 tttagtagct tctcaatgtg atcctgtttt tagtgcgttt taccagaaaa aagtttccga
     2401 aggaaaacat catctaactg ccttaggtgc ggtttctcgt aagctttgtt acgtgattca
     2461 tgctattttg actaaaaacg agccttatat aattgtttct gattagctat tgaacattca
     2521 aaaaaactaa tggtaagaag cttatttgtt gtaccctttt ttcggaatta tctgttataa
     2581 aatatttttt gatttggcta atttttactt gactctatat agttagtctc tccaataaat
     2641 atagtatctc cataggcttt ttttaaattc tcttcatt
//
LOCUS       DAYLZO010000215         2663 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.227, whole genome shotgun sequence.
ACCESSION   DAYLZO010000215 DAYLZO010000000
VERSION     DAYLZO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.227"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            65..592
                     /locus_tag="ACGVJG_003032"
     CDS             65..592
                     /locus_tag="ACGVJG_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFJ6558914.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            613..1803
                     /locus_tag="ACGVJG_003033"
     CDS             613..1803
                     /locus_tag="ACGVJG_003033"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFJ6558915.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            1843..>2663
                     /locus_tag="ACGVJG_003034"
     CDS             1843..>2663
                     /locus_tag="ACGVJG_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HFJ6558916.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTK"
BASE COUNT          932 a          474 c          597 g          660 t
ORIGIN      
        1 aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc tataatgaaa
       61 aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg aaaaaacgat
      121 taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga tcagctcgat
      181 tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag tagaaatcgc
      241 aaagaaatat gcaaatcttg aggaaaccga tcatttttat tggtttactc gaaagaagac
      301 atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta tcattcctaa
      361 atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa acgaagcaaa
      421 acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt tagggatgta
      481 tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac tgaattatta
      541 cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt aaaaggtaaa
      601 ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc tgtcactttg
      661 gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct gagtttgact
      721 gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat cgaagcaatc
      781 cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct tcgacaggca
      841 atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca aacagtcgta
      901 acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga cccgcaagat
      961 gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa actggcagaa
     1021 ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac tgttgaacag
     1081 ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc atcgaatccg
     1141 actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc agtaaaaaat
     1201 gatattttga tagttgctga tgatatttat ggccgtttag tttataatgg caatgagttt
     1261 actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat caacggcgtg
     1321 tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa tcctgtgatc
     1381 atcaatggaa tgatcgctgt tgcttcccaa tcaactagca atccgacagc agtcagtcaa
     1441 tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat gcggaacgcc
     1501 tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccggg tgtttctctt
     1561 gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct acgcatgtgt
     1621 aagtatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg ggtagcgctt
     1681 gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc cactgatcgc
     1741 gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa aagtcaaaat
     1801 taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca aattcaaatc
     1861 atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt cgcaaataag
     1921 cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt tttccaaggt
     1981 gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa tctacaccaa
     2041 gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa atttggttat
     2101 gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc gatcacacca
     2161 aaagaacacg gaacagattt cttgatggat catcgtcatt tatggctgcg ttcttcaaga
     2221 caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga attctttaat
     2281 caacatcatt ttgtcaaaat cgatccgccg atcttgactg gttctgcacc agaaggcact
     2341 acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca aagtggccaa
     2401 ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg tccgactttc
     2461 cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat cgaaccagaa
     2521 atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt agcattctta
     2581 gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg agacaaagaa
     2641 gtcttaaaaa cttatacaaa att
//
LOCUS       DAYLZO010000216         2603 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.228, whole genome shotgun sequence.
ACCESSION   DAYLZO010000216 DAYLZO010000000
VERSION     DAYLZO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.228"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(24..599)
                     /locus_tag="ACGVJG_003035"
     CDS             complement(24..599)
                     /locus_tag="ACGVJG_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFJ6558917.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(604..1578)
                     /locus_tag="ACGVJG_003036"
     CDS             complement(604..1578)
                     /locus_tag="ACGVJG_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFJ6558918.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1602..1646)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1861..>2603
                     /locus_tag="ACGVJG_003037"
     CDS             1861..>2603
                     /locus_tag="ACGVJG_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFJ6558919.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASA"
BASE COUNT          829 a          508 c          508 g          758 t
ORIGIN      
        1 tttgctaatc cagctctgtt tattcagcct atttttcttt ttgaatatat caagcgttgt
       61 ggcaacatag tgatgactaa tgaaacaacg acgatagaca acaaacgatc aaggtagtct
      121 gtacctgcct gtaccaagat cacacttgtt gtctgactta aacctaaccc atgtaatagc
      181 tgaacgatca tactggatcc tgaggaggtg atcccgccaa ataactgaac ggtaatgatc
      241 gaagaaacga tcgttccagg taccgacaaa atcaaagcga gtaataacag tttcaagctg
      301 tttttatctt tgccgagata tcttggcaaa atcaatccag caagcaagcc tgtgagaatt
      361 tggattggcg aataatacag agaaaaaata tctgtagtca ctccacttaa aataccgctg
      421 gacaatcctg tcagcattcc aggtacaggc ccaagcaatg caccagcaaa aatcgttccg
      481 atagaatcga gatagattgg caaacgaagc cataaagcaa tcgttcctcc aatataattg
      541 atcccaatac acaaagccat cactgtaagt acgcgagttg ccattttgtt tttattcatt
      601 ttgttagccc atctttaacc tttccaaatc tttcataatc gtttcttctt gtgcatttaa
      661 aagaacggtt aaaaactttc ggaaaaaaag acgagtatct acttttgtca taattttact
      721 gttagcaggt ttctgccaaa attcataacg atcaaccaat gtttgtccac gacttattcc
      781 ttgagtttcc acagcagtga agcttttgaa accttcacag atggcctcct cgataaaata
      841 agcaacagct aacggatcat tgatgacaca tcctaaaatt ctttcttgtt tccagtggaa
      901 gtcaaaataa aaacgagtga tcgcttttag atactctccc tcttcaggat tcatttgaca
      961 gcagtattcg aggatagtcg gtgtcaagac gatttctctt gtcacatcta acccaaccat
     1021 ttctatcgtt tgtttgagat tctcgaatac ataagaagct gcttcaggat cacaccagta
     1081 attgtattca gcaactggcg aacagttacc atggcttttg taagttccac ccatcgagac
     1141 aaaacgttcc atatgtttcc caatatttgg gttgactttc aaagcagaag cgatattggt
     1201 caaaggaccc aaagcaatga ctgatgtatt tgtcttttct ttgaaataat cagccaaaaa
     1261 atcaacggca tggagaggtt ctgcctgttt tctcaagatc atcgggaaat ttgtttcccc
     1321 taaaccatcc ataccatgag tgtcttgtgc actgatgaaa ggttgcttca aaggtttttc
     1381 cattccttga taaacaggaa tgtctaaacg atcacagcgt tccaggcatt tcagcacatt
     1441 ctgcgtacct atatctgcag ggacattccc acaaactacg gtaatagcaa ctacttcgat
     1501 atcaggggat ttgacagcta atgataaagc taaagtgtcg tcgatccctg gatcgcaatc
     1561 aataattact tttcgcatta tttccaatct cctttcttta gagatactta gttttttatt
     1621 ctgggaagtt tccgaaccag tccaagacga gcgtcttgat tataatgcac gaaattagta
     1681 tactggcttt tattgcgaat gtaaatgctc tcttacaagt aaatctattg aggagaactt
     1741 taagtaaatg atacaatgaa aaagaaatgg aatttacgtg tgccgaaaat aaagaatcct
     1801 atggatcggt acattggata atagatagat tatagtttgg cagaaagcgg cgggtgtaca
     1861 atggttattt tttatttcat tcttattctg ataattatag ggatcagtta ccattttctt
     1921 tcttattttt atgcaaagaa attagtaaga gcgggagtac agagaggata tgactggtat
     1981 gaagacactg gtcatcgttt gatggaccct gctgcagtca cgtcagtaga aaaaaaacga
     2041 caaaaattag aagaagagtg tgaaagcttt tgggagcagg gaacacccaa aacaatcaaa
     2101 agttatgacg ggttgaaact agctggtcag atgttcatcc atccaaataa gcagaataaa
     2161 tgggtcattt gcgtacatga ttaccgaagc acagggaaaa gagacatgtc tcatataggt
     2221 aaaagatatg cagagaaagg attcaacgtt cttgtacctg atttacgtgc gcacggagaa
     2281 agtgaaggag aaatcatcgg aatgggatgg ctagatcggc tagatttaat tgcctggatc
     2341 caactcatct tagatgaaca gccgaatgcg tccattattt tacacggcgg atcaatggga
     2401 gcttctacga tcatgatggc aagtggtgaa aaacttccat cagcggtcaa aggatttatt
     2461 ttagacagcg gatatgtttc tgtttatgca gaattccgat atatgttaag taaaatcact
     2521 gtctttccta aaaaaatggt catgagatat gcgaatcatt atgcccagaa atacgccggt
     2581 tactccttaa agcaagcatc agc
//
LOCUS       DAYLZO010000217         2572 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.229, whole genome shotgun sequence.
ACCESSION   DAYLZO010000217 DAYLZO010000000
VERSION     DAYLZO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.229"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..569)
                     /locus_tag="ACGVJG_003038"
     CDS             complement(<1..569)
                     /locus_tag="ACGVJG_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558920.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSARYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLI"
     gene            complement(682..939)
                     /locus_tag="ACGVJG_003039"
     CDS             complement(682..939)
                     /locus_tag="ACGVJG_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558921.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(1536..1814)
                     /locus_tag="ACGVJG_003040"
     CDS             complement(1536..1814)
                     /locus_tag="ACGVJG_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558922.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(1848..2099)
                     /locus_tag="ACGVJG_003041"
     CDS             complement(1848..2099)
                     /locus_tag="ACGVJG_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558923.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT          797 a          519 c          345 g          911 t
ORIGIN      
        1 ttgattaagg taattccgta ttgtgtcaaa acttcttgcg cttctccgaa attaagtaca
       61 gttcttatgt atccactttc tcctaatata aagtctacct ctcttttaaa accttcctcg
      121 agatttctaa gggctatatg tggttttaat gacattgatt gtacattttc ccccatataa
      181 tatcgcgcac ttcctcgact aaaatagatt cctagatcgt ccccaaaagg tagatcttct
      241 ctaccactag ctaatctact tctccatctt cgttctcttt tcatagaacc tgcgctgaac
      301 tcacacccgt taaactcagc tatgacagta tcttcaatta tgttaatctg tccatctagt
      361 tcgtattcaa tcctaaatgg aaattctcca taggtgattt ccggttcggg tattttaggt
      421 gagattaatg caaagaaagg aaatattaag cttggcaaca tcaaaaaaac tcctcctatg
      481 atcacaattc ccgatgtaat ccaaaatata atttgtacaa ttctcattga tccattgtat
      541 tccattttat tccccctatt actattcata tccgcctatc tatatcgatc ttccaatgca
      601 gagataattg cttccatacc ttgatttttt actgagttac ctatgcgaga tatcattctc
      661 cactaaggtg catcatattg tctatctaac atgattaatt ctccaatagg tgtatactcc
      721 tccaaaaaca acatttaatg catctccctc gacaacataa gctaccatgt ttgctgtata
      781 tgtgtttgag tctaattcat aagcagataa atttactgtc gctggattaa atataatcgc
      841 attcgtattt atagctactg aatttgctgc ttcagcacca ccttttgaat gtccgatcat
      901 tgtgatttca atagtaggat ttacgtggac agccctcacc gtttaccctg accattatag
      961 aaatcgctta cttttttgaa ctacgcaaca aaggatacaa tgctcctaac ttcactgtct
     1021 ccatttttgt ccactaagga atataaaaaa taggcattaa ttcatttaat tcggtcacaa
     1081 cacgttacct tttaaatcat tatactgtat ctcatacatt attatcaata aactattata
     1141 aaaatgcaaa aaatgcttta tcaatctatt ttgtaattgg tcataatatc attgaaatca
     1201 taccctgtat cgatttttcc tatgaatctc gtctatttgt gattgacgaa aaagaggcaa
     1261 gacagacact ggaatggtaa ttttacccac tattttgctc caactatttg taatcgtacc
     1321 cacttttgaa ataccgagat gatcatttta attgatatgc tgattcccaa gcaaaacccc
     1381 aagaaattaa atgctctatc gcatctagct tcttgggttt tgcttatata aattccaaca
     1441 tttaaatttt ttatgctgtt cggtctcaga aatgccgttt ttgcttatat cacaatatca
     1501 agatgtcaaa ttattcgtgt tgccgatgac tttgattaat ttttttcaaa ttgtttatta
     1561 agactaatgc tgtctttttg cagataccaa tgttcgaact ttttaacatt atcatatgat
     1621 cctgcaagtt ttaacttctt accattagat gttatcaaag ttataattaa ccttgccttt
     1681 ttcccattag gatcttttat tttatgaatt ttctgcaggt aatctctcct aatggcagct
     1741 atttttacct catcagtgct gataaaccat tgatcatcca catacatact gcctgttatg
     1801 ttgtgttttt ccatttcttc cgagaacgaa aagtttaagg ctcgttctta cttccaaagt
     1861 tgaatgagtc catggattgg acttagtgtg agaattgaga acaacaataa gaatccatat
     1921 cctcccgcaa acatccctaa ggctatactg tctctccatc cataagtagg attagaagag
     1981 aaaagagtca ccgtttgtaa taatatgaaa aggaataatc cagcacaaac accaaagata
     2041 acaactccaa acattgaatt tttatacaat gaccatttat ttataggctt ttctttcatt
     2101 ttacttcccc ctactgttct gttcacacgg aatgaattca aagttcataa gccctcctct
     2161 ttgaacatat gaatacgact tgctaactat actttaaaaa agcttgtttt aagcggttat
     2221 aggcgagttg taatctctat tcatctcatt agcaaataac ttgccgaaaa gtctactgaa
     2281 attaaagcag atagtggaag aattatttag ccaggataga cactatttgt gaaacacatg
     2341 tctttacttt tgaaattcta tgatgaaaag ccttggaata cagtagctct taattggtaa
     2401 tactttttcc attataaata acctcttttc attttatatt tatcccctct aaaaataata
     2461 tctaatctaa tattttaaac ttatatttcc ctcgctgtgc taatagagca ttactaaata
     2521 aatagaaact agaaaaaagc attacgcctg ccagaataaa ccaattattt gt
//
LOCUS       DAYLZO010000218         2556 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.230, whole genome shotgun sequence.
ACCESSION   DAYLZO010000218 DAYLZO010000000
VERSION     DAYLZO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.230"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1438
                     /locus_tag="ACGVJG_003042"
     CDS             <1..1438
                     /locus_tag="ACGVJG_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFJ6558924.1"
                     /translation="SKKNQPKDIKDIVDTRMYKERDDLNYTLIINKDGNYSWRPLVLL
                     HPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILN
                     WWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLG
                     DKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRY
                     RDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEI
                     KASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKI
                     TSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALK
                     PNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEG
                     TIKDEEKYIKMSKKIYITEFDDFNDYPF"
     gene            1776..>2556
                     /locus_tag="ACGVJG_003043"
     CDS             1776..>2556
                     /locus_tag="ACGVJG_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFJ6558925.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTS"
BASE COUNT          982 a          365 c          441 g          768 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtcta
//
LOCUS       DAYLZO010000219         2531 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.231, whole genome shotgun sequence.
ACCESSION   DAYLZO010000219 DAYLZO010000000
VERSION     DAYLZO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.231"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1130
                     /locus_tag="ACGVJG_003044"
     CDS             <1..1130
                     /locus_tag="ACGVJG_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFJ6558926.1"
                     /translation="GEQGGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGN
                     GKAIKITGASENNLKNISVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSA
                     KPGKFKTISGYESIEKIIDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGY
                     KKGRFSFNVKGGRCEACRGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKS
                     IADILEMTVEDAVEFFKHIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLAS
                     ELHKISNGKNFYILDEPTTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADY
                     IIDLGPEGGEGGGTILATGTPEEIINVKESYTGHYLKKIMV"
     gene            1393..>2531
                     /locus_tag="ACGVJG_003045"
     CDS             1393..>2531
                     /locus_tag="ACGVJG_003045"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFJ6558927.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIK"
BASE COUNT          877 a          382 c          548 g          724 t
ORIGIN      
        1 ccggagaaca aggcggacaa atcgtggctg caggcactcc tgaagaagta gctaatgatg
       61 aaaattcttt gacagggaaa tatctttcag ggaaaaaatc aatccctgta ccaaaagaac
      121 gccgtaaagg taatgggaaa gcaatcaaga tcactggtgc ttcagaaaac aatctgaaaa
      181 atattagtgt tgaatttcca ttaggagaat ttgtagcagt cacaggtgtg tctggttctg
      241 gaaagagtac attggtcaat agtatcttaa agaaatcgtt agcgcaaaaa ttaaataaga
      301 attctgctaa gccaggtaaa ttcaagacaa tttccggcta cgaaagtatc gaaaagatca
      361 tcgatatcga tcaaagccca atcggccgga cgccgagaag taatccagcg acttatacaa
      421 gtgtatttga tgatatccgt gggttatttg ctcaaacgaa cgaggcaaaa atgcggggtt
      481 ataagaaagg gcgttttagt ttcaacgtaa aaggcggtcg ttgtgaagct tgtcgcgggg
      541 atggaattat taagatcgaa atgcactttt tgcctgatgt ctatgttcct tgtgaagtat
      601 gtcatggcaa acgatataac tctgaaacat tagaagtgca ttacaaagga aaaagcattg
      661 ctgatatttt ggaaatgaca gtagaagatg ctgtagaatt cttcaagcac attccaaaga
      721 ttcatcgcaa actgcaaacg attgttgatg ttggcttagg ttatgtgacg atggggcaac
      781 cagcaacgac attgtccggt ggtgaggcac aacggatgaa acttgccagt gaattgcaca
      841 aaatctctaa tggaaagaat ttctatatac tagatgaacc aacgacagga cttcatagcg
      901 atgacatcgc ccgcttgttg catgtattac aaagattagt agatgctggt aacacagttt
      961 tagtgattga acacaatcta gatgtaatca aaacagcaga ttatatcatt gatttaggac
     1021 cagaaggtgg agaaggtgga ggaacgatcc ttgcgactgg aacaccagaa gaaatcatta
     1081 acgtaaaaga aagttataca ggtcactatt tgaaaaaaat aatggtataa agaggataga
     1141 tagctgagat agttaaaaaa acataaaaaa atgaattttt gcttgtttta ttaatatcag
     1201 tgttctagaa tcattacatg gtagttcttt ctacactgat tggtatagac aaaaacttcc
     1261 tataagttca atccgttcaa aaaagaattg agcaatcgga ggagtttcct ttgattgctt
     1321 ttttctttat ggtatgatat tccttgtgta gttggaaaag aaaaacaaga gattatgagt
     1381 aagtgagaga taatgatgaa cagtattgaa aatgaagaat ttatccctat attattagga
     1441 agcgacatga acgtttatgg tatggcacgt tcttttaatg aagcatacgg caaaatctgc
     1501 caagcatatg cttcggatca gttagcccct acccgctaca gtaaaattgt caatgtagaa
     1561 gttattccag gatttgataa agaccctgtg tttatcgaga cgatgcttcg tttggcaaaa
     1621 gaaagatatt ctgataagag taaaaaatat cttttgattg cttgtggtga tggatatgca
     1681 gaattgatct cccaacataa acaagagctg tcagaatatt ttatctgtcc atatattgat
     1741 tattctttat ttgaaagact gatcaataaa gtaagctttt atgaagtttg tgaagaatat
     1801 gatcttccgt atcctaaaac attgatcgtc agagaagaaa tgttagtaaa tggacatttg
     1861 gaacaggaat tgccttttga attcccagtt gctttgaaac ctgcaaacag tgtggagtat
     1921 ctttctgtcc aatttgaagg aagaaaaaaa gcttttattt tagagactag agaagagttc
     1981 gatttaattt taggccggat atacgaagcc ggttataaaa gtgaaatgat tgtacaagat
     2041 tttatcccgg gtgatgatag taatatgcgt gtgctaaatg cgtatgtaga tgaagatcat
     2101 caagttcgga tgatgtgtct tggtcatcca ctgttagaag atcctacacc agcatcgatt
     2161 ggtaattacg ttgtgatcat gccagattat aacgaaaaaa tctatcaaac cattaaggca
     2221 tttttggaaa aaatcgaata tacaggcttt gccaatttcg atatgaagta tgatcctaga
     2281 gatggagaat ataaattatt tgaaatcaat ttgagacaag gtcgtagcag tttctttgtg
     2341 acattgaacg gcttgaatct ggctcgtttc gtcacagaag atcgagtgtt taacaaaccc
     2401 tttgttgaaa cgacctatgg aacgaatcaa tcagataaag caagattatg gatgggtgtg
     2461 cccaaaaaaa tctttttaga atatgctcgg gaaaatgaag acaaaaaact agctgagcaa
     2521 atgatcaaag a
//
LOCUS       DAYLZO010000220         2530 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.232, whole genome shotgun sequence.
ACCESSION   DAYLZO010000220 DAYLZO010000000
VERSION     DAYLZO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.232"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..1148)
                     /locus_tag="ACGVJG_003046"
     CDS             complement(<1..1148)
                     /locus_tag="ACGVJG_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFJ6558928.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACP"
     gene            complement(1148..1357)
                     /gene="copZ"
                     /locus_tag="ACGVJG_003047"
     CDS             complement(1148..1357)
                     /gene="copZ"
                     /locus_tag="ACGVJG_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFJ6558929.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(1370..1810)
                     /locus_tag="ACGVJG_003048"
     CDS             complement(1370..1810)
                     /locus_tag="ACGVJG_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFJ6558930.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(1884..2423)
                     /locus_tag="ACGVJG_003049"
     CDS             complement(1884..2423)
                     /locus_tag="ACGVJG_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFJ6558931.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT          649 a          561 c          442 g          878 t
ORIGIN      
        1 ggacacgcaa tgactaatac agagacacta tggatcaatg cgagttgcca atcaccagtg
       61 atcaaacctg tagctatgag cgtgataaac gcgatgacta atacgatcgg aacaaaaatc
      121 cctgagatcc gatctgcgat tttttggatt ggggctttgc tcccttgagc atcttccacc
      181 atttgaatga tttgcgctaa tactgtctgc ttgccgactt gtgtaacttc catgtgaagt
      241 agaccattcg tattgattgt cccgccaaag agcgtgtcat ccggattctt ttctacagga
      301 agattttcac cagtaagcat actttcatct atcgcactgc ttcctgaaat aattcgacca
      361 tctgctggaa tctgctcacc aggacggatg actagttgat caccaacgac tacttcttca
      421 atcgctaacg tttcttcttt cccgtttctt atcacttggg cggtttttgt ttgaagagac
      481 atcaattgtt tgattgcatt acctgttttc gtttttgctg tatgttccaa atatttgccg
      541 agcaaaatca gcgtgatgat catactgctg ctttcaaaat aaagatcttg ggggtgtcca
      601 cggaaaaatc cgttgtaaat gctcaatgca aaggcagccg atgttccaat tgctaccaat
      661 acatccatat ttggtgcttt cgtcttgatt gcatggtacg cacctttata aaaacgagca
      721 ccgacataaa attggacagg agcagacagc actaactgga cgattggaaa atgaaaaaag
      781 tgaacaatcg ccgcatggct tcctaacatc atagcaatca ttgacaacat caacggcaaa
      841 gtcaaaaccg tactcaatat aagatcgaat agcatttttt tcaaatacgc ttgcttttct
      901 tctgcaattt tttgtttgtg ggcttcatcg tacaaaattg caccatagcc gatgttttct
      961 acgctttgga ttaatttttc agtggtcgtc ccttcatatt tgacacttgc tttttcagtg
     1021 gccaagttga ctgtggcatt gaccacacca ggttgtccgt tcagctcttt ttcgatccta
     1081 gcagaacaat ttgcacaagt catacctgta atgacgaacg tatccatttt ggtttctgct
     1141 gttgccatca aatcacctct gctttataac ccaatccatt gattgcttga caaatctttt
     1201 cggattgaac ttttgtttca tcaaacttga ccgtaccgtt tgcttttttc aaatttactt
     1261 tcactttttg cacgccgtca agtgcgctta ctgtttcttc tactcttgct gcacaatgat
     1321 tgcagctcat accttcgatt gaaaattgtt gtttcatttt ttcttcctcc tattgatgtt
     1381 gtttacattc acattgacct gggatacagt tacattcgat cgtatctact ggttgctttt
     1441 gttccagttg tttcaaaatc tcattgatat cttcttgagt caaagtggct tcttctacta
     1501 gatccgcaat cgtctctcca atcctttttg cacagatatg ggaaaaaaga ttttccgtcg
     1561 cactgcgaac tgtttccatc tccgagacag ctggataata aatgaatttt tttccttctt
     1621 gttctgtcca gatcacttct ttttttacca aacgacccag caaagttttg atcgttgctg
     1681 gtttccaatc catcgaatca gctaagatct gcgtgatttc ttgggctgtc gtctgtccaa
     1741 gtgtccaaat aacacgcatg atctcccact ctgaatcact gatctttact ggcgttttga
     1801 ctttttccat ttatttcaca cctttcgttt acaaatgtaa tcattatctt atgaatacag
     1861 attacacttg taaacaaaaa ctgtcaatta tttttgctct ctattttcaa tttagttaaa
     1921 aattcttgtt tctctcgtct tgttttaagg atgatcagtt tatttcttgc attagctact
     1981 tggatcagct gctgtatttt cggttcattg ttttcttcaa aatgccagat aaaagataaa
     2041 aattctaagt attccctgtt tagtttctcc gaaaatgatt caggcatatc tggccgagat
     2101 tttttgttta tctttctttt tagagaacgc caaactactc ggtagatggc gagataacgc
     2161 ggcactttaa gccaaatgat cttatctgct tctttaatcc tttcctctaa tgtagaagta
     2221 taatttcctt caacgatcca tgacggatta gataccataa acaattgctg ttcttgcaaa
     2281 aaccactttt ttgcctgaat actgtagtct gttgtatgcc ataaaaggtc tagcggcagt
     2341 agcggaaagt cggtgatttc tttgatcttc cttgcgaggg tcgattttcc cgacccactt
     2401 tgtccaatca cgatgatctt cattcttctc cctcacttct ctttggaatt tggttcgatc
     2461 ataccactta taacgccgca aaaaaacaag ggactgtgac aaaagtcgtg tcaaagtccc
     2521 ttgtttcgaa
//
LOCUS       DAYLZO010000221         2507 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.233, whole genome shotgun sequence.
ACCESSION   DAYLZO010000221 DAYLZO010000000
VERSION     DAYLZO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.233"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            175..597
                     /locus_tag="ACGVJG_003050"
     CDS             175..597
                     /locus_tag="ACGVJG_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558932.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            607..810
                     /locus_tag="ACGVJG_003051"
     CDS             607..810
                     /locus_tag="ACGVJG_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558933.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1021..1605
                     /locus_tag="ACGVJG_003052"
     CDS             1021..1605
                     /locus_tag="ACGVJG_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFJ6558934.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1774..>2507
                     /locus_tag="ACGVJG_003053"
     CDS             1774..>2507
                     /locus_tag="ACGVJG_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFJ6558935.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIG"
BASE COUNT          883 a          360 c          472 g          792 t
ORIGIN      
        1 gtcacttttc tttttaaatg gcaatatttt ttgtaaataa atgtcaatca agaaaattta
       61 aacgattatg acattaaaaa tatattttat gaattttcta gtatataact tgatttatct
      121 gttaagattt attaagaaaa cgttttctta tttttgattt cgaggtgata gacaatgaaa
      181 aataagttta ttttatcttt aggtatttta actattggaa ttagtgtaac tggaactaca
      241 attcctacat ttgctgatag tacaaatgaa ccaactactg tagtagataa taaccaagaa
      301 ttaactcctt tagataggaa cggaatagcc attggtaacc caactaacga cttaactgtt
      361 actgaaaatt atttaatagt ttctccatta cttgctcgag cagcttctaa aacgatacga
      421 gtgacaggaa cgatgatttt tgataaagtt ggttggcctt ctagacctcc tcaatcacgg
      481 aattggaaag aaaaaagaaa tggttattgg tactatggaa aattgtattt acaaagttat
      541 aaatcagctg gaaacaattg gattgcgact tattcaggaa ccttaactag aaagtaggga
      601 ttttttatgc cagaatttgt gaacacacca aatttttatc aagatagcga tattctaagt
      661 aaaattaatt taccgaaaga agaatcagct ttacaagttc agcgaacttt acaggagaca
      721 caaaaaattc aaaaatcaat ttctaatcta gaagattctg aagaaaaaag aaaaaaaaga
      781 gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa aaaagagtgt ctagtcgaca
      841 ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac tttctttttt
      901 tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg
      961 ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt
     1021 ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac
     1081 agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt
     1141 aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt
     1201 tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg
     1261 aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt
     1321 tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga gcgtaatttg
     1381 atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg gtttggtggt
     1441 cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt ggatagcggt
     1501 acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat
     1561 ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt gtcatttaat
     1621 gctgaaaatt tttgccattt atctagaaaa gtgacaatag tatgagaatt ttttgtaagt
     1681 gtctactgat aacttattct caaatattta gctatttctt ctttacaaca tcgaacatat
     1741 gtttgtataa tgtggaagaa tggagtgtta tttatgatgg attattcaaa agagccagta
     1801 aatgattact ttttaatcga tatgaaaagc ttttacgcaa gtgtcgaatg tatagaaaga
     1861 aatttagatc cattaacaac agaacttgtt gttatgagtc gatctgataa tactggttca
     1921 ggattgatat tagcttcttc tcctgaagca aaaaagcggt atggtattac aaatgtgagt
     1981 agaccacgtg acttaccaca accatttcct aaaacactac acgttgttcc accacgtatg
     2041 aacctatata tcaagcgaaa tatgcaggta aataatattt tcagaagata tgtggctgat
     2101 gaagatctac tgatttactc gatcgatgaa tcgattttaa aagtgacaaa atcactgaat
     2161 ctttttacga ctgaagaaac acgaagtcag cgaaggaaga agctcgctca aatgatccaa
     2221 gaacgtatta aagaagagtt aggtttgatt gctacggtag gcgtcggcga taatcccctt
     2281 ttggctaaat tagccttaga caatgaagca aagcataatg aaggatttat tgcggaatgg
     2341 acgtacgaaa acgttcctga gaaagtatgg aatattcccg aaatgacgga cttttgggga
     2401 attggttcgc gaatgaaaaa aaggctcaac caaatgggaa ttttgagcat tagagacctt
     2461 gctaactgga atccttatac gattaagaat agattaggtg tgattgg
//
LOCUS       DAYLZO010000222         2454 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.234, whole genome shotgun sequence.
ACCESSION   DAYLZO010000222 DAYLZO010000000
VERSION     DAYLZO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.234"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1445
                     /locus_tag="ACGVJG_003054"
     CDS             <1..1445
                     /locus_tag="ACGVJG_003054"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFJ6558936.1"
                     /translation="KGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPNLDNKDFAA
                     HMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRLSIAWTRLF
                     PTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGWVERRVIDD
                     FVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQDIYQALHH
                     QFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLENNFYADVQV
                     KGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESADPNAERTPG
                     NTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAVDQVVDGKI
                     HDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYGFIYVDADD
                     EGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            1482..2324
                     /locus_tag="ACGVJG_003055"
     CDS             1482..2324
                     /locus_tag="ACGVJG_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFJ6558937.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT          833 a          393 c          533 g          695 t
ORIGIN      
        1 caaaaggatt tttatggggc ggcgcgatag cagcaaatca agtagaaggt gcttggaatg
       61 tagacggtaa aggtctttca gtagcggata tggctactta taaaccaaat ttagacaaca
      121 aagattttgc tgctcatatg catgtcaccc tgcagtctat aaaagaagct agtgaagaca
      181 aatctgataa atggtaccca aaaagaaggg gcgttgattt ttatcatcac tataaagaag
      241 atttagcttt atttgccgaa atgggcttta aaaccttgcg cctctctatt gcttggactc
      301 gcttgttccc tacaggagaa gagctggaac cgaatgaaaa aggggttgca ttttatgaaa
      361 acgtctttaa agaaatgcaa cgtttaggaa ttgagccgat tgtgacacta tctcattatg
      421 aaatgccatt agctttagca gttaaatata atggctgggt ggagcgtcga gtaattgatg
      481 attttgttcg cttcgctaac gtttgttttg atcgcttcgg caaatacgta aaatattggc
      541 tgacatttaa tgagatagac agcatccacc gtcatccatt tacaacagct ggaattatcc
      601 aagagaaaag cgcagaaggt caagaagctc aagatatcta tcaagcattg catcatcaat
      661 ttgttgcgag tgcgttagtg acaaaagatg ctcatgaaaa aattccagga agccaagttg
      721 gttgtatgtt aacgaaattg atgacttatc cattaacttg cgcaccagaa gacgttgagc
      781 tgaccttgaa aaagaactta gaaaataatt tttatgcaga tgttcaagtt aagggagaat
      841 atcctgctct aatcgaaaag gatttggaaa atcggggaat tcagattaaa atggaagatg
      901 aagatcgaga aattttaaag aaacataccg tagattttgt ttctttcagc tattacatgt
      961 ccatggcaga atcagctgat ccaaacgcag aacgcacacc gggaaatact gttttagggg
     1021 ttaagaatcc tcatttgcct tcaacagatt ggggctggca aatcgatcca aaaggtttga
     1081 aaatttcgtt gatcgaactt tatgatcgtt accaagtacc gttgatgatt gtggaaaatg
     1141 gtatgggagc agtcgatcag gtagtagatg gtaaaatcca tgatccgtac cgcatcgact
     1201 actttaaagc acacttccaa caaatgaaag aagcaatcga tgaaggtgta gatttgatag
     1261 cttatactag ctggggacca attgatttag ttagtgcagg aacttctcaa atgtctaaac
     1321 gatatggatt tatttatgtg gatgcagacg atgaaggaaa tgggacatat aaccgcttga
     1381 aaaaggattc gttcaactgg tacaaagaag tgatcgaaac aaacggtgca tccttattcg
     1441 agtagtctcg atctttttaa gcagcgatag ggggataatg agtgatttca ataaaaaaag
     1501 tattgaattc aagtgtggta cttgttgaag aaaatggcaa agaaatgatt gcattaggta
     1561 aagggatagg ctttggacag aaagctgggg atgaaatatc aggagataaa gtagataaaa
     1621 tatttctgcc ttttgaagaa aagaaatctt cacaactatt agagttagtt gaagaaatac
     1681 ctattgaatt ttttgaaatc actcgtacta tcgtttttga agcagaaaaa ggattagata
     1741 aaaaactgaa tcctggagtc tatttaacct taacagacca cctgcatttt gcagttgaac
     1801 gcgcacaaaa aggggtaaat attgcaaacc gtttgtactg ggaaataaaa atctactatc
     1861 ccaaagaatt tcaaattggg aagtacgcac tcaccctttt gaacgagcaa tatggaattg
     1921 atcttcctaa agaggaagcc tcaaacattg ctttccattt gatcaatgca caatcggaag
     1981 atcctgatag cggcgctgga ttcaagtatg caaaaatgat cggcgggatc gtcaatttgg
     2041 tccggtattc gttgcagaaa gatatcgata ctaattcgat ccattatgcc cgcttcatta
     2101 cacatgtacg tttctttgta gaaagatatt attctgatgc tttgttggat gatggtgagg
     2161 aagaattcta caatcaaatg tggatcttat acccgtctgc catggaaagc gcagctaaga
     2221 tcaaagacta cattgaaaaa gtttatcata aaacaattcc gaacgatgaa gttgtatacc
     2281 taggtgtcca catcaatcgg ttgatgaaac atgctaatgg gtgagcgctc agtaatacta
     2341 ttgatgcttt taaaatcttt agagatggat tatagtaaaa acaaggtgat ttccagctgt
     2401 ttgttatcta taagagacat ttaaaaagga ttaacgtaag ataataaaaa atct
//
LOCUS       DAYLZO010000223         2453 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.235, whole genome shotgun sequence.
ACCESSION   DAYLZO010000223 DAYLZO010000000
VERSION     DAYLZO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.235"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..218
                     /locus_tag="ACGVJG_003056"
     CDS             <1..218
                     /locus_tag="ACGVJG_003056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558938.1"
                     /translation="RSDRMNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISK
                     LIIYRDTERFLTVLGNVYENPELLEVE"
     gene            219..680
                     /locus_tag="ACGVJG_003057"
     CDS             219..680
                     /locus_tag="ACGVJG_003057"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFJ6558939.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            706..1158
                     /locus_tag="ACGVJG_003058"
     CDS             706..1158
                     /locus_tag="ACGVJG_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558940.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            1264..1458
                     /locus_tag="ACGVJG_003059"
     CDS             1264..1458
                     /locus_tag="ACGVJG_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558941.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            1489..1782
                     /locus_tag="ACGVJG_003060"
     CDS             1489..1782
                     /locus_tag="ACGVJG_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558942.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            1713..2252
                     /locus_tag="ACGVJG_003061"
     CDS             1713..2252
                     /locus_tag="ACGVJG_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFJ6558943.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            2327..>2453
                     /locus_tag="ACGVJG_003062"
     CDS             2327..>2453
                     /locus_tag="ACGVJG_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFJ6558944.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQ"
BASE COUNT          870 a          363 c          561 g          659 t
ORIGIN      
        1 aaaggagcga tcgcatgaat ttgactaaac aagaaaaaag aatctgtcgt cagataatgt
       61 tgcaaattat aggtgatcgt gacatggacg aattatctca acaagagagt cgaatggttc
      121 ggaagataag taagctaatt atttatcggg atacagaaag atttctcact gttttaggta
      181 acgtctacga gaatccagag ttattggagg tagagtaatt gccaaaaata ttagatgcat
      241 gttgcggtag tagattgttc tggtttgata agaacaattc tgacgtaaca tttatggatt
      301 gtcgtcagca gtatgaagaa ttatcaacag gtcatgtgat caatgttgat ccagatgtgg
      361 tcgcagactt tcgcgatatg ccttttgaag atgatgtgtt cgatatggtt gtatttgacc
      421 cgccacactt aagacatgtc ggcgaaaact catggcttgc caaaaaatat ggaaaattag
      481 acgaactttg gccagaagac atcaggcaag gttttgcaga gtgtatgcga gttttacgac
      541 cttcaggaac attaattttt aagtggaacg aggaacagat accactttct gatgtgttag
      601 aggctattgg agaacagcca ttgtttggta ataaacgaag caaaacacat tggcttgtgt
      661 ttatgaaaaa attcagctaa cggaggaaac aaaggaggaa aagtaatgaa gattgtatta
      721 aataaatgtt ttggtggctt tgggttgagc catgaagcaa aaatggaaat ttttaaaaga
      781 aaaaatattg aagtattccc atacatcaat aactttagct atgactcaga tgatgaatac
      841 acgagataca caggccaaaa gctaggttcg atggatttta tctactattt caaaaaagac
      901 ccgaaaatcg acaaagttac aggaatttat agcgaaattg accgactgta cggaattgct
      961 gatgattcca gtttttcaag tgattcaaac agaggtgata aggacctcgt tgctgttgtc
     1021 gaaaaactag gaagtgaggc cagtggacca tatgcaagtc ttaaagtcgt tgatataccc
     1081 gatggggctg agtgggaaat aagtgattat gacggcgttg aaactgcaca ttatggtttt
     1141 caaacaggta gtatttagtc cactaatgac aactaaagag gaggcagaag gatgagtgaa
     1201 gatgaatact ttgaacagta gtctattaac gacagagatt tttaactgga aaggggtgta
     1261 gtcatggacg catcggaagt agtaaataaa attaatagtc tcaaagaggt tttcggagac
     1321 gtcgaagtag tgataaacgc acaaggctat gacaacgaat tttttaagca gattaccgac
     1381 attagcattc agcaaggaat ggaagacgaa gacggaaact ttatcgatga agtagccatt
     1441 ttagtgactt gcgagtaagt aattgacagc taaagaggag gaagcggaat gaaactaaaa
     1501 gacggatttt actccagcgg ccacggcatc ggcggtttaa tgctagatat gccgacaaag
     1561 aatcctaaaa cacgtaagaa accaaaattc aaagtcggtg acatggttcg ctgcgaagca
     1621 gaaggattca tctatccatt tcgtggatat gtagaacacg tctataatca ctcagcaatc
     1681 attcgcattg aaaacacgat ggaatgtgat aagtggttag cgaaaagcaa agagaattta
     1741 gcagtggtga gattggtgga catggaagtt ataaaatctt aattttgctt ttttacaact
     1801 atcctacaac aatgagcgtt attccacaac tggaaggaga agaggcaatt gtgaacattt
     1861 tagagaatat agatattaag caaacaagga aaaatgctag acgattacta aaaagataca
     1921 gaaatttaga acgtttggtc ggaccagtga aaatagactt ttccgtgatg actgttacca
     1981 aaaatttgaa attcacaatt gacagtcaaa acgaagaaat cattgaagcg ataagtactc
     2041 gagattcggt tatcgaagca ttaacacggc taagtagaat ccatttccag gtactttatt
     2101 atagttattg ttttcctaat aagatgtcaa tgtatcaaat aggagaaaaa ttaggatatt
     2161 ccgatagaac tatcgagaga atgaaggcag tagctttggt tgaatttgct gaggcgtata
     2221 aatcaggaga actcatttca cgtacaaaat aaaaaaagtc ggattcctcc gactatgaat
     2281 aatatttccg acataagtat tatatcataa ttgggggaat cagaggatgg tacttttcga
     2341 tgtaaagaaa tatgagacac caagcgcgaa agatgttgat atggaacgca caaaacataa
     2401 tgtcgctgtg tttctttcag catatctatc agctagatgt agagtagggc aac
//
LOCUS       DAYLZO010000224         2404 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.236, whole genome shotgun sequence.
ACCESSION   DAYLZO010000224 DAYLZO010000000
VERSION     DAYLZO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.236"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..744
                     /locus_tag="ACGVJG_003063"
     CDS             <1..744
                     /locus_tag="ACGVJG_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFJ6558945.1"
                     /translation="FMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYA
                     YLVPFNSKNKQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLT
                     EEFEFDPNGRTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGV
                     WASDYNAMARKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIP
                     ERKEAEVISEENEDVQTGLFDASNPPLNK"
     gene            787..1587
                     /locus_tag="ACGVJG_003064"
     CDS             787..1587
                     /locus_tag="ACGVJG_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFJ6558946.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            1602..>2404
                     /locus_tag="ACGVJG_003065"
     CDS             1602..>2404
                     /locus_tag="ACGVJG_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6558947.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFS"
BASE COUNT          879 a          397 c          535 g          593 t
ORIGIN      
        1 tttatgtcga atgttatgac tcttgtatct aatgacagtt atcttgcaga tagtgaaccg
       61 atgtctatca tgagtggtgc gttaactgct gcaacattaa atcttgggct agataagaat
      121 ttaggttatg catatttagt tccattcaat agtaaaaaca agcaaacagg aaaatgggaa
      181 aagaaagctc aattcatgct tggctataaa ggatatatcc aattagccca acgatcaggt
      241 aaatacaaag cattaaatgt gatcgaagtt tacgaaggag aactaaaaag ctggaaccga
      301 ctgacagaag agtttgagtt tgatccaaat ggtagaacgt ctgatgaagt cattggatat
      361 gttggctatt tcgagttact gaatggattc aaaaaaactg tctattggac caaacaagaa
      421 atcgaagctc atcggattgc taacaataaa gatcgcgata agacaaagtt aagtggtgtg
      481 tgggcatctg attacaatgc aatggcacga aaaactgttt tgagaaatct tctttctaaa
      541 tggggaatct tgtccatcga aatgcaagaa gccaccacat cggatgagag agtccaaagg
      601 gttcaagaag acggcagcat tattgctgaa acagaagttg aagaagatat tcctgaaaga
      661 aaagaagcag aggttatatc tgaagaaaat gaagatgtac aaactggatt atttgatgca
      721 tctaatccgc cgttaaacaa ataatgaggg agctttctcc cttgcttttc tagaggagaa
      781 atacgaatga acacaggata tgtcaaatta catcgaaaag tgatgaattc attcgtttgg
      841 accaatcctt atatgtataa attatggaac ttgtgtttga tgaaagcgag tcatgagaat
      901 cgcaagatac tttttaatgg aaaagagata caagtgaaca gcggagaatt cgtcacaggg
      961 cgcgatgcta tcacatctga gatgaacgaa ggcgtgaaac gtgaacatca agtgaacagc
     1021 ggttctgtat ggagatggct aaaacaattt gaaaaaaacg gaatgttgaa catcaaatca
     1081 actacgaaat acagcgttat atcaataaaa aactggtctt tgtaccaaag cagtgaacaa
     1141 caaatgaaca tcaaacgcac aacaagtgaa caacaagtgc acacaaacaa gaatgaaaag
     1201 aattataaga atgaaaagaa taataaaaca tcatcgtcgc aaccacgcaa aaagcgtgtt
     1261 tacgacaccg actcagttta ctacattctc gcggaggagt tattcaaaca gatttgtcag
     1321 aatcaggaaa tcaaaaagcc gaatctgcaa agttgggcag atgacattcg gaaaatgatc
     1381 gagatcgaca agcggaccga aaaccaggta cggggaatga ttgaatggag tcagcacaat
     1441 gtgttttggg catcgaatat tttatctgct aaaaaattgc gagaaaaata cgacacaatg
     1501 gcagcgcaag caaatcgtga ttataaaaca aaacaaacta aaacgcttga atacgagaaa
     1561 tttagcacag atgagttgcc tatttgagag gaggcgtagg catggaaact gttggggaaa
     1621 tcatggaaaa gctgatacag aaagtgcttg tccaacgtgg cgaatgtcct gaatgcggac
     1681 agcctttgta tggatggcgt acgaagaacc ctgatggctc agaacgttgt aagccaacct
     1741 gtatgcaatg tgggtataaa gcattgcgag ttcaggaaga cttacaaaca gaacgaattt
     1801 acaacgagag cctgaaagca agagcaatca attttttcaa aggtggttct gttgtgccta
     1861 atcaagcgtt gtttgattgc acattgcaga attatcaaat tgtcgatcaa gaaacaagac
     1921 aagcggttga agtaaccaaa cgctttgtta attcagtctt gttaggaaat ccaagtcacc
     1981 ttgttttaac tggaaaacaa ggaacaggta aaagtcacct agcgatggca gcggcttggg
     2041 aagtcttgaa gcgatcaaac tacgataaaa aaatcttatt tatcgggtta caggaaatgc
     2101 tggatcaaat caaattttcg tacaacaatc ctgaactcag aaaaacgatt gagggatcgt
     2161 tgattgcaga tatcaaaaca gcggatttag tcatcattga tgatatcggt tcagaactag
     2221 gaaaagatgc atcagatagt cgagcgtttg gcataaacac gctaaattcg ttcttggatg
     2281 cacgacagaa cctagcaacg attatcacaa cgaacttgct tggtgaagaa ctgaaaaaag
     2341 cttacggtac gagaacaata tcaagaatgt ttgtcaactc tgatggattt acgatggtat
     2401 tttc
//
LOCUS       DAYLZO010000225         2398 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.237, whole genome shotgun sequence.
ACCESSION   DAYLZO010000225 DAYLZO010000000
VERSION     DAYLZO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.237"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            207..2069
                     /locus_tag="ACGVJG_003066"
     CDS             207..2069
                     /locus_tag="ACGVJG_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFJ6558948.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACGVJG_003067"
     CDS             2069..>2398
                     /locus_tag="ACGVJG_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFJ6558949.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAYLZO010000226         2385 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.238, whole genome shotgun sequence.
ACCESSION   DAYLZO010000226 DAYLZO010000000
VERSION     DAYLZO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.238"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(365..1657)
                     /locus_tag="ACGVJG_003068"
     CDS             complement(365..1657)
                     /locus_tag="ACGVJG_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFJ6558950.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACGVJG_003069"
     CDS             complement(1931..2191)
                     /locus_tag="ACGVJG_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558951.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          805 a          444 c          362 g          774 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
     2341 gtaaaatatt ttgtgtaaat gaaaaaatcc atacaaaaaa ggaag
//
LOCUS       DAYLZO010000227         2385 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.239, whole genome shotgun sequence.
ACCESSION   DAYLZO010000227 DAYLZO010000000
VERSION     DAYLZO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.239"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..473
                     /gene="dctP"
                     /locus_tag="ACGVJG_003070"
     CDS             <1..473
                     /gene="dctP"
                     /locus_tag="ACGVJG_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HFJ6558952.1"
                     /translation="QSETAIKMVELLGGSPVPMGSDEVYTSLQSNLINGSENNEFVLY
                     TAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAKESTVFEIEAFAK
                     AIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLYQKIQEQGA"
     gene            479..958
                     /locus_tag="ACGVJG_003071"
     CDS             479..958
                     /locus_tag="ACGVJG_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFJ6558953.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            974..2269
                     /locus_tag="ACGVJG_003072"
     CDS             974..2269
                     /locus_tag="ACGVJG_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFJ6558954.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            2280..>2385
                     /locus_tag="ACGVJG_003073"
     CDS             2280..>2385
                     /locus_tag="ACGVJG_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HFJ6558955.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLA"
BASE COUNT          661 a          418 c          553 g          753 t
ORIGIN      
        1 tgcaaagcga aaccgctatc aaaatggtag agcttttagg cggttcgccc gtacctatgg
       61 gaagtgatga agtgtacacg tctcttcaat ccaatttgat caacggatca gaaaacaatg
      121 aatttgtctt gtatacagct ggacatggtg gtgtggctaa atactattcc tatgatgagc
      181 atacacgtgt gccagatatc atcatcatga atgatgccat caaagaacag ctaactgatg
      241 agcaggaaaa agccattgaa gaagcagcga aagaatcaac ggtttttgaa attgaagctt
      301 ttgcaaaagc aattgaagaa gaaaaagctt tagcacaaga agaacatgga gtccaattca
      361 atgatgtaga taatacaccg tttcgagagg cagttgcccc acttcatgat atattcaaaa
      421 acgatcctac ctacaaagac ctttatcaaa agatccaaga gcaaggagcg tagctgtgat
      481 gaaaatgatt ttaaacaagg tattagagat cctgggagct gttttgcttg ttgggatggt
      541 cgctgttgtt ttatggcaag tattttccag aacggtttta ggaaatccaa atacggtcac
      601 ggaagagctt gttcgattcg gtcttgtctg gtttgccatg ttgtcttccg cgtatgttgt
      661 gggacaaaaa gcacatttag ccgtaacggt tttaagtgaa aaattaactg gcaaaaaaag
      721 taaaatttta gaagttgttg ttcaaagttt atttctggta tttgctgcga tgatcatggt
      781 gtatggcgga tggaatgctg tcacattgac aatggggcag atctcgcctt ctttaagtat
      841 tcctatggga tatgtttatc tgtcagtacc tgtatcgggg gtactcatca ttatctatag
      901 cctgatcaat ctaatcgata cattaagcaa caagccagca attcaagaaa aaatataaaa
      961 atggaggaat aaaatggcaa tagaagccgg attagtgtta ttttttgtat ttgttgtgtt
     1021 gcttgtatta gggatgccga ttgctataag tattgcattc tcatcgatga tgactttatt
     1081 gttagttata ccgtttgatg tttctgcttt tagttcagct caaaaaatgg tgggaagtat
     1141 caatagtttt tcgctgatcg ctattccgtt tttcgttctc tcgggcatta tcatgaataa
     1201 tggagggata gctaagaaac tagtggattt tgcgatgttg ttcgtcgggc gcgtgccggg
     1261 ggctttggct catacgaatg tgttagggaa tgctttattt ggttccatct ctagctcggc
     1321 cattgccgct tctaccgcga ttggcggagt gttgatccct cagcaagtgg aagaaggata
     1381 tgaccggaag tttgcgacag cggtcaatat cgcgtcagct ccgacaggta tggtcattcc
     1441 tccaagtact gcttttatca tgtattcact tgttgctggt ggtgcttcga tctctgcttt
     1501 gtttatggga ggttatctcg taggcgcttt gtgggcgcta ggaatcatgc tagtagcatt
     1561 catcgttgct aaaaagaata aataccctgt tgtcgaaaaa ggacagatgg ctcatgctgg
     1621 aaaaataata aaagaggcga ttcctagcct tctattgatt gtcatcatta tcggtggtat
     1681 cttgacgggg atatttacag cagtagaagc ttcagcgata gctgtcattt actccctgtt
     1741 gattgctatg ttttactata aaacggtaaa actaaaagat atacctaata tgttgtttca
     1801 atcagtagca atgtcaggga cgatcatgtt cttattggct acttcttcca tgatgtcttt
     1861 cgccatggcg tttaccggta ttcctcaggc aattagttcg ttgattatgg gcgtaacgga
     1921 taataaattt attattctgt tattagtcaa tcttgtgtta ttactggtgg gtatgtttat
     1981 ggatgtggcg cctgctatct tgatttttac accaatattt ttaccaatcg tcacaagtgt
     2041 aggggttgat cctgtacatt acgggttgtt ttcaatcatg aatctgtgtg taggctccat
     2101 cacgccgcca gtaggtacag gactatatgt cggtgcaagt gtaggaggcg taaaagcaga
     2161 ggcaatgctg aaaccgcttc ttccttttta tggggttatc ttagtcgtct tgtttttgat
     2221 tacttacttc ccgcaaatcg tcatgtggct acccaatatg ctgagttagg agaatagtta
     2281 tgttgaacat tacaattaga ggacatgatc tttccgaagt ccaaacgata gaagatcttg
     2341 cagaaaaaac aaaagaacaa gggattcata cactgcagct cgcct
//
LOCUS       DAYLZO010000228         2371 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.240, whole genome shotgun sequence.
ACCESSION   DAYLZO010000228 DAYLZO010000000
VERSION     DAYLZO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.240"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..649)
                     /locus_tag="ACGVJG_003074"
     CDS             complement(<1..649)
                     /locus_tag="ACGVJG_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFJ6558956.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADL"
     gene            complement(933..2036)
                     /locus_tag="ACGVJG_003075"
     CDS             complement(933..2036)
                     /locus_tag="ACGVJG_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFJ6558957.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          671 a          514 c          405 g          781 t
ORIGIN      
        1 tcaaatcagc cgtgaatgtt ccagctgctg ctttcggcac tttcagtgtg atatttgctg
       61 gatcataaac agaaagccat gttcccagac cttgatcttt tgctgctgta aatattggtg
      121 tcgctgcact tgctttactc aacccagtta cagtttgact agttggtcca gtggattcgt
      181 tatctgacgt acttttaaca gtggatgttc caaaatctaa ttcagcgcct tgcaaaactt
      241 gtgaaccgtt tacaaaatct gaaaccgaaa ctgtcacgtt ccacccttgt gcttggccat
      301 ctgcacctac acctcgacga tctgatactt ggagataagg tgtatcgttt tttgaagcgt
      361 ctacttgata cgtttttgtt ccgctttcaa tctcgtgtgt accaaatggc aattcaggaa
      421 ctacatccaa tgttaaagaa cctgtctggc cagttgctgg attttccgga tctgctggct
      481 ctactggttt tgaaggatca gtagggttaa gaggctcaac ggtatttgtt ggttcagtaa
      541 aggtaatatc tccttttgaa gtcgcttgac cctcagcaaa agctggtgtt gccaagccgc
      601 ctagaagaag tgttgaaagt aataatgtcc caatcatgtt ctttttcata ataaatcact
      661 cctttttctt agtgacctaa gtatagtaga gacagtatgc aaaaaatcct catgaattca
      721 tccgcttact tgaaattatg accctttttg tataaaagag aaaaaaacaa taaaatcgtt
      781 tacatgaata ttcttccttt tttattaagt atgaaaaaat atttcaattt tattttttcg
      841 tttcaaatta ttctattagt gacatccttg aactataaaa ataggagtac ggcatcatct
      901 gccgcactcc tatttttttc gtcatttatt tcttaaataa aacgattctc acgcaatttt
      961 atttttttga tatcttctac tgtttgtgtt ccagctaatt gcatgaccat ttccaattct
     1021 gttttaaaga aatcaaacac ttgatggact cctgtacttc ctcctaaaga caatccataa
     1081 ataacgggtc tgccaatagc aacaagatcg gcacctgatg cgattgcttt gaaaacatgt
     1141 tgtccacgtc gtacaccgct gtcaaacacg attggtacac gtttatctac tgcttctgcc
     1201 acatattgca acgaatcaaa cgcagcaggc cccccatcta attgacggcc accatggtta
     1261 gatacccaaa ttccaccagc accagattct aatgatcgat agacatcttc ttctgattga
     1321 acacctttca cgtagactgg caaatcagaa tgagcagcga taaattctac atcttttgga
     1381 ctaagttttt gtttggaaga tttatataca gcatccatcg tttggcctac tcctgattga
     1441 taagcctgaa cgattggcat tggcagcggg aaggtaaacc cgtttctgcg gtctgtttca
     1501 cggtttccgc caactgttgc atccgctgtc aatacgattg cttttgctcc attacgcttg
     1561 gccacttcaa gaatatccaa attgattcca ttatctttgc tcatataaaa ttgaaaccat
     1621 tgcggtgctt tctccccgcc tgcttctctt atctcttcta aagtacaaga agcgtaagaa
     1681 cttgctgtat agatcgtccc aaaatctgct acaccttttg cagaagcttt ctccgccttc
     1741 acatgagcta atccatgagc agcaactggt gccatgatga ttggtgcagt cagcatttct
     1801 tcgtcaaaat gtgtagttgt atcgggtaat tctacatctc ttaatacatg aggaataatc
     1861 aaacgatgat tgaatgcccg ttcattttct tgataagtaa ataagtcgcc agcaccactg
     1921 ctgatgtaac cataaccacc agctggaatc acttgtgaag ccgcgatttc taaatcttcc
     1981 atattgataa aatctactat tccttcattt gtacctgctt gatatgtttt ttccatctca
     2041 atgccttctc tctataaaat ttgaatgatg aacaatatga tagcgtttac actttttata
     2101 ttactcttcg acattgatac aggcaaataa tctgtttaca tttcagccat tccagatgtc
     2161 actactctgg aagaaatgat tgtaataaaa atagataaaa aaccgatggg actttgacaa
     2221 aagtagtgtc aaagtccctt tttttgaata aacggtgttc aaaagccatt ttctcggtat
     2281 taagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttactactcg
     2341 aaactaagcg actttttcac aacctctcga t
//
LOCUS       DAYLZO010000229         2314 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.241, whole genome shotgun sequence.
ACCESSION   DAYLZO010000229 DAYLZO010000000
VERSION     DAYLZO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.241"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(31..810)
                     /locus_tag="ACGVJG_003076"
     CDS             complement(31..810)
                     /locus_tag="ACGVJG_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558958.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(1192..>2314)
                     /gene="glyS"
                     /locus_tag="ACGVJG_003077"
     CDS             complement(1192..>2314)
                     /gene="glyS"
                     /locus_tag="ACGVJG_003077"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFJ6558959.1"
                     /translation="LTARLEDAEFFYNEDKKASIDQYVEKLKTVTFHEKIGSIYEKMQ
                     RVQVIARIIGEQVGLSEEELMDLQRAAEIYKFDLVTNMVGEFPELQGIMGEKYALLKG
                     EKPAVATAIREHYMPISSEGELPQSTVGSVLAIADKLDSIFSFFAVGMIPSGSNDPYA
                     LRRQAYGVVRIIESKKWAFPLSVLQETISDVISKDTDRFGIGLSEGQQQVIDFIKGRL
                     RQLLTTKNIRHDVIDAVLNAEQKDLTKVFAAAQLFKQHLADEDFKPSMEALTRVVNLA
                     KKAQLEKQSEVDPELFENDAEKELHKAVEAMKASFADQTIHENYDQLVALRPLIENYF
                     DQTMVMADEPAVRKNRLTQLSQIAAMALSLASLDQIITK"
BASE COUNT          639 a          463 c          416 g          796 t
ORIGIN      
        1 ctaaccttgc ttgaatcaac gttttaactg tcaatagctt atattgctat agcccatttg
       61 ctgagcaaca gtttgtgctt ttgttgcgta tgcggctctt attttttcat tccggatctg
      121 ctctatttga ctgagcgccg tcaaatattg atcgtaagta gcggtagctg ttacttgacc
      181 gtctttcagc atcgtatcga atagctgatc tagttgcgtg acttgtgcaa tttgggtatc
      241 agcgaatcct agatattgca taacgactgt tttccaagca ctatccttaa aaaagctcat
      301 attttcacgt acatgggcga catctgcact tgccaacttt tctttgatga tgacatcatc
      361 cacagcttgc tgccagtttg atacgttttt agtaaataat ttattgacag ccgtctggat
      421 cttcagtttt gattctgcat cctctaactg cgttaagact gctttctttt cttcttggat
      481 cgcagcagct tctttaggta attctgattc cttgatgtca taatcatctg ccgaaagttt
      541 gatagaagat agtttatgtg acaaatttga aactgtttct tctgttattt caggatttag
      601 gaaaattttt tcttttgtac tgtacagctt atctacttct tttgataaag aggtcacggt
      661 gtctttttga ttggtgatgg cagattctgc gtaattgatc cgttgatgat attgccgctg
      721 attattgaga tggtatccaa acaagcaaat cacaatagcg cctgtgccta ccgccacagt
      781 agccaataac ttgacgaacg gcttttccat agattcactc ctaaatattg atgatagata
      841 agtaaagatg tgattcattc acatcagaat gagcgctttt tattttaaaa gcataattaa
      901 actaatatat tcatagaaat atttataaac aggttttccc tatataatca taccacatac
      961 actctctttt tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta
     1021 taattaatcg ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca
     1081 aagaataaaa taacaaaaat tattaaaata gaattaataa cagctattta taacaaaaaa
     1141 aacagagaca cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt
     1201 gatgatttga tccaagcttg ctaatgacaa agccatggca gctatttgac ttagttgtgt
     1261 tagtcggttc ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt
     1321 ttcgatcagt ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc
     1381 aaaacttgct ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa
     1441 cagctcaggg tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac
     1501 acgtgtcaaa gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa
     1561 ttgtgctgct gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat
     1621 gacatcatga cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc
     1681 gatcacttgt tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat
     1741 aacatcgcta atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat
     1801 gatccgaacg acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg
     1861 aatcatccca actgcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac
     1921 agaacctact gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg
     1981 aatcgctgtc gctacagcag gtttctcgcc tttcagaaga gcatattttt cgcccatgat
     2041 cccttgtagt tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc
     2101 agcagctcgt tgcaaatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat
     2161 acgggcaatg acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa
     2221 agtaacagtt tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata
     2281 aaagaattcc gcatcttcta aacgagccgt caat
//
LOCUS       DAYLZO010000230         2283 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.242, whole genome shotgun sequence.
ACCESSION   DAYLZO010000230 DAYLZO010000000
VERSION     DAYLZO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.242"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            581..1540
                     /locus_tag="ACGVJG_003078"
     CDS             581..1540
                     /locus_tag="ACGVJG_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFJ6558960.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1527..2132)
                     /locus_tag="ACGVJG_003079"
     CDS             complement(1527..2132)
                     /locus_tag="ACGVJG_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558961.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
BASE COUNT          775 a          366 c          346 g          796 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttaccttcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
      541 aaaatagttc cgtcgaaaga attataagga gtgagtacaa atgacctata cccatcttac
      601 accaactgaa ctcgtaatga tagaagctta tttcaatcaa tcacactctg tttcaaaagt
      661 agcccatctg gtacaacgtt ctcgacaaac catttataat gtctatcgtt ttcttaaatc
      721 tggtggaagc gcaataagct actatgaaca atataagaaa aacaaacgta actgcggaag
      781 acgtccgatt gtgttaccag atgggcaaca agaatacatt caaaaaaagg tggctcaagg
      841 gtggactcca gatgtcctta ttggtcgagc agagtttcct attgcttgtt cggtacgtac
      901 actatatcgg atgtttaaag ataaaacctt tgaccctcac gatttaccaa tgaaagggaa
      961 aagaaaacca aacggacata aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca
     1021 ggatagaaca actgattata tacagttcaa tcatgagttt ggtcacctag aaggtgacac
     1081 cattgtcggt ggaaaacata aaagtgcggt gatcacgtta gttgagcgat tatctaaagt
     1141 gatcatcacc ttgaaacccg ctggtaggca agctatagat attgagaata gactgaatca
     1201 atggtttcaa tctgtaccga aacacttgtt taaatccatc acatttgatt gtggaaaaga
     1261 gttctcgaat tggaaaaaga ttagtaatac caatgatatt tcgatttatt tcgctgatcc
     1321 aggaacacct tctcaacgag gactaaatga acattcgaat ggcttattac gtaaagatgg
     1381 cttatataaa gctatggatt ttaataaggt atccgaaacc tttatccaat cggtggcatc
     1441 aaaacgaaat catattccta gaaaatcatt acactatcgc acaccattgg aagtattttt
     1501 gagttacgta ggtaaagatg atttgtctag cttaatttga caaacgaaac tttgtaaaaa
     1561 tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat gtcaatattt
     1621 ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa ttccctgtaa
     1681 ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc acccattcca
     1741 ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc agtttgttta
     1801 tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc aaaaattttt
     1861 tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac tgataattga
     1921 tactctatat tacatgactt aattagtttt ttagatcccc cctccagata ggcatatgtt
     1981 tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc ataaattaaa
     2041 aatgactgtc tatttagccc ataatctaat acaacttctt catactcata tttctttaaa
     2101 tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt gttatttatt
     2161 gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt agtgaactac
     2221 tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc ttgggaagag
     2281 agc
//
LOCUS       DAYLZO010000231         2212 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.245, whole genome shotgun sequence.
ACCESSION   DAYLZO010000231 DAYLZO010000000
VERSION     DAYLZO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.245"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..716
                     /locus_tag="ACGVJG_003080"
     CDS             <1..716
                     /locus_tag="ACGVJG_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558962.1"
                     /translation="RDSMKLYEVSNKQVKEVSLPEGAPVDVVTALGSELTGGGLNFSS
                     NGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKDSFENPEQWPIYLFALPENQ
                     TMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSSELAAQESKEYNKLLDKKFI
                     DQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDRRQVLNALADDVIINGGKVF
                     ILDQKDAPDEKSLTAILRY"
     gene            1219..1539
                     /locus_tag="ACGVJG_003081"
     CDS             1219..1539
                     /locus_tag="ACGVJG_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558963.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            1992..2192
                     /locus_tag="ACGVJG_003082"
     CDS             1992..2192
                     /locus_tag="ACGVJG_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFJ6558964.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT          742 a          369 c          454 g          647 t
ORIGIN      
        1 accgcgactc catgaaattg tatgaagtca gcaacaaaca agtaaaagaa gtatctctac
       61 cagaaggggc accggttgat gtcgttactg cattgggatc tgaactgaca ggtggcggtt
      121 tgaacttctc ttctaatgga ggaggcggag cccgagatgg agtaactttc catggcgtaa
      181 atccaaaaga tgaagaagta gagatcgatt gggtcaacta ttaccaagca gttgatacgt
      241 acttgaagga ttctttcgaa aatccagaac aatggccgat ttatttattt gctctaccag
      301 aaaatcagac gatgttcaaa aaaattgcta aaaatcccta ttattgtgca gacgttgcga
      361 tttctgcttc tcctgcgcag ctatccttta gagatatcga agaaggggcg aagaagctgt
      421 caagtgaact cgcagctcaa gaatcaaaag aatacaataa actcttagat aagaaattca
      481 tcgatcagtt agttgatatc gttccagctg cgaaagaagg aaaagtgtca catttattta
      541 tcgctacgtc aaacttagct gatggtttcg gtgaagatcc tgaaacagaa tatgatcggc
      601 gtcaagtgtt gaatgcatta gcagatgatg tgatcattaa tggcggaaaa gtgtttattt
      661 tggatcaaaa agatgcgcca gatgaaaaga gtctgacagc gattcttcgt tattaaaaga
      721 ggttgtgaaa tcagtgtttt gacctgagta ttagagaaac aaacggaaaa atagtttcac
      781 atattttgcc gttttttgag ctaataccga aggtcagctg attaaacacc gtgtatgaga
      841 ggttgtgaaa aagctgttct gcatcaagta ataagaacag caagcaaaaa tagctcttca
      901 tattttttgt ttggtgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
      961 gaggttgtga cagaagtggt cagcttcaag aaataagaag agattgacaa aaattatttc
     1021 tcaaattttt gtgaaatttc ggcttatttc cgaagaagct acttctggaa caccgtttaa
     1081 aaaagaggtt gtgacgtggt cacgacctct ttttgacgat tatgatacaa aaaacatcat
     1141 ttatgaacaa aatgtgaata acgggcgttc ttgagatatg attttaattg ggaaaaactt
     1201 atcgataagg aggtctttat gaaaaaaaga ctgttatggt gtttgctgtc tctatcccta
     1261 agtttaacaa gtcagccagt ttttgctgaa gaaacacagc ggtttcctat tacaggcaca
     1321 cgtctgtaca aaaatgttta tgtacagaca ccagatcttg attctgcgat tcctgaaaca
     1381 gaagaacggt ctttgcagcc aagatttggt tacttttggt actgtccgac ctgtgattat
     1441 gaaagttgct ggcatttctt acattctaca gctgcagctt ttgcttttac tcatactcag
     1501 aaatatgatg gacatcatcc tgttatttat gaggtataga tatgagcaaa aaagaaaaac
     1561 taatctcgtt ggtcgtattg gtttgccttg ttcttctggt aactgtcata ggctatcaga
     1621 aataccatga gaacatcaga cctactaaag aatcttcccc agcctcgacc gttgaaacag
     1681 aaccgataag tagtcagaaa gaataatttt tttagtttaa aaaagaacat cagagcaaaa
     1741 aaacaccaac tgaacaatat gtcgtttatt ttaaaaaaaa gttcggtttt tcactttatg
     1801 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
     1861 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
     1921 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
     1981 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
     2041 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     2101 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     2161 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa ta
//
LOCUS       DAYLZO010000232         2207 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.246, whole genome shotgun sequence.
ACCESSION   DAYLZO010000232 DAYLZO010000000
VERSION     DAYLZO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.246"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..172)
                     /locus_tag="ACGVJG_003083"
     CDS             complement(<1..172)
                     /locus_tag="ACGVJG_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFJ6558965.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAES"
     gene            complement(156..620)
                     /locus_tag="ACGVJG_003084"
     CDS             complement(156..620)
                     /locus_tag="ACGVJG_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFJ6558966.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(1107..>2207)
                     /locus_tag="ACGVJG_003085"
     CDS             complement(1107..>2207)
                     /locus_tag="ACGVJG_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFJ6558967.1"
                     /translation="VSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLT
                     DGQTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVM
                     IAQAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYP
                     SYKESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLN
                     NVIETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQA
                     PNSGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNE
                     KTGTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
BASE COUNT          632 a          420 c          411 g          744 t
ORIGIN      
        1 cgctttcagc gaccacaaag cgccatggtt tcatattgac agaagaaggt gcagtcgtcg
       61 cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca tcatatacgc
      121 gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc attattatta
      181 acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc taaaagtgtt
      241 agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc tgtatcttta
      301 tggtccattt cacaatgctt gattttcttc tgggcttttt ctgttagcgc tacatacact
      361 tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc caataccttc
      421 agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact gctttgtagc
      481 aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact tataccgata
      541 ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata taaaaggtga
      601 ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc aacgattgac
      661 ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa
      721 cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg gaaaaaatga
      781 taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat agaagatagc
      841 tcttctgcag tagataattt tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat
      901 cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa gttatgacaa
      961 gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa
     1021 gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg gctgttctta ctacttgatg
     1081 cagggcagct ttttcactac ctctttttaa taaccataaa atcctattcc ttcatatctc
     1141 cagcccatcg gtgttagcca atctctttcc ttagcactca aggtatagtg atgggtacca
     1201 gttttttcat ttggattaaa tagtcgataa attggcttgg aaccaccaga ataaaaactt
     1261 agaccttcat aacgccaacc gatttttgtt aaagagttga tctcagattg tgccattgtg
     1321 taatgatggt ctccactgta aggattgtat aaacggtaga caggttgtcc gctgtttggt
     1381 gcttgccaag caatcccttc atattcccag cctacagttg gcagataatt tttttctccg
     1441 gcattcaatg tatataaatg ttctcctgta tgacggttat acagacgata catcggagaa
     1501 gttgaagttg gtgctgtatc atactgtgtc agattatagg tttcgattac attgttcaat
     1561 gtagaaccat aattaggcgc agtggcataa cgacctgtta gccaagccgt agcatctttg
     1621 taagagttcg tattgctttt ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa
     1681 gtcgtcttca ataccgttac gttatcttgc aatgattctt tgtacgaagg atattttcta
     1741 aaagcggcat tcaccgtaat ccattgccca ttcacatatt ccgaagtagg catcgtaaca
     1801 gattggccat tatagcttcc tttaatccca aatagattgt aattaggggc agaagccaaa
     1861 gtactgttcc cccagccact ttcaacgatg gcttgagcaa tcatgactga tgcatataag
     1921 tcattggcac ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga
     1981 gaattagcag atgagcgagc ataaaaagca tagccagtat agttttcgaa ttcagacaaa
     2041 tctgcttgat gggtatgtgt caatgtttgc ccatctgtca attggttcgt gatttcttct
     2101 gcattctctg gattgttctg agcttctgct gatgtactgt cttcggtttg tgtttgttct
     2161 gttgtttcag ttgttggttc ttcggttgaa attgtatttt ctgagac
//
LOCUS       DAYLZO010000233         2202 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.247, whole genome shotgun sequence.
ACCESSION   DAYLZO010000233 DAYLZO010000000
VERSION     DAYLZO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.247"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..470
                     /locus_tag="ACGVJG_003086"
     CDS             <1..470
                     /locus_tag="ACGVJG_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFJ6558968.1"
                     /translation="ASFYGTKEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRK
                     ASGTVNTAEDSMLVTTIPYDKGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYL
                     PEGFTIGAILFVLCIALFILYVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(561..1175)
                     /locus_tag="ACGVJG_003087"
     CDS             complement(561..1175)
                     /locus_tag="ACGVJG_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6558969.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            1295..1834
                     /locus_tag="ACGVJG_003088"
     CDS             1295..1834
                     /locus_tag="ACGVJG_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558970.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            1831..2169
                     /locus_tag="ACGVJG_003089"
     CDS             1831..2169
                     /locus_tag="ACGVJG_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFJ6558971.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT          758 a          351 c          398 g          695 t
ORIGIN      
        1 ctgcaagttt ttatggaacg aaagaagtaa gtttcatgga gcctaaagtc gttggtctag
       61 atgttgtagc ctatcaagcc gcaatgaatc agatcaaaga aaaaggtgtc gaaatggaag
      121 ccactggcag aaaagcttct gggactgtaa ataccgctga agattccatg ttggtgacaa
      181 ctattcctta tgacaaagga tggaaagcta aaattgatgg gaaaccagtc aaggtcagca
      241 atttcaaaga cgcattcttg atgataaaag ttccagcagg taaacatact gtcactttca
      301 gttacttgcc tgaaggattt acgataggtg caatcctttt tgtcctatgt attgcattgt
      361 ttattcttta tgtctggtac cttgaccgca atcgcccctt ttatggcacg attgatccag
      421 caatagaaga aaaaccgcga aatacacaca gaagaagacg aagaaaataa agaaacgatg
      481 gaatctgtgt cactcttcta tttaaatggc aaaaatttca atctttaaat gacaaaaacc
      541 acctgatttt tggtggtttt ttatatacct atgactaaac tatctcttgc tgcttcgaca
      601 acttctacag tgatcttatc catttgattg attcttaaaa tccgatcaat ctgcgcaaat
      661 aaacgatgaa taagcctaaa atttcctttt gtgatcttaa tgatagttgt gatagcctca
      721 taatcagaaa aatcttctag cttgatatca attcctaaat ctgcccattt gtattccaaa
      781 atatgatgtg tttcgtcctt actaaggtta tcaaattcat gtgcaaatcc tatgcgagag
      841 taaagctgcg gataacgaga caatcttttt tcaataccag gcataccgat aagaaccatt
      901 gccagatcat ttctatcgta aatatctctt agctgttcta aatgctgaag ctttaaacga
      961 tcaatttcat cgacaataat taagtcaatt gcttcatagg ttttaggcac aggaggatct
     1021 tcttgattgt attttagtac catatgcatt gctttagtca gacctattct actagttatt
     1081 gtgttaatat ccgaactgac acgagacatc ttttcagctg gtacagtata aaaaacttta
     1141 tttacactta atatttcatc tgttgcattt atcaagaaca agaaaaagtc tgaatattca
     1201 aaaaaaataa cggtgtacat ttttaaaagg agatgtataa tcgagattta gaaagcgttt
     1261 tcctcaaaat ttaatgaatt aggaagtgat tactatgaaa aaattattta gcgcactttt
     1321 tattctgtta tttggcttta ttggtattgg gacttctgga aatatagtta aagctagcac
     1381 gatggttgaa caaacaagtg aaatggcaaa aacttttagt caattaacac cagaagaaca
     1441 aagttacttc aaatccaaag gatttagtga taaagatact ttttacactc aaaagataga
     1501 gcctcaagat ccgaaaacac gtttttctgc aaatgtattg cggttaaccg catctactaa
     1561 aaaaattact aatacgcagg ggcggacaga atatattata acaagtccca attcggcgat
     1621 gtatcgtatt tctacacgct tgagtttagg tagtgcaaaa tcatatactc aaacagtaaa
     1681 tctagctaat tcttatggtt atggtggagg aatgtatttt acatttactg ggaaaagaca
     1741 atattacagt gtaaaattag ctatgcaagt ttataataca tttggtgtag ggtcagtctc
     1801 ttgcactgcg ggaggattga caataggaaa atgataattt cttactatgt cttaaataga
     1861 agtgatgatg aaggactctt cgtttttcaa tcattacatg aggatacgat gctaaaagag
     1921 ttttataatc ggattaagaa taaacagata gaaacaaatt tatatttttt aagtgactct
     1981 atcttgcctt ttattagcaa ccctaaaatt cttaacttgt tgcagacaaa agatttgaaa
     2041 agtttaccga ttattgtcat tgatgaggaa attgtaaaat atggcgctta tctaagcgta
     2101 gaagaattag aaaaattaac taacattgga atgagtattc aggaggataa tggggtacat
     2161 ggaacataga atgaatatgc acagttgaga acggaatttg ac
//
LOCUS       DAYLZO010000234         2201 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.248, whole genome shotgun sequence.
ACCESSION   DAYLZO010000234 DAYLZO010000000
VERSION     DAYLZO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.248"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(98..1228)
                     /locus_tag="ACGVJG_003090"
     CDS             complement(98..1228)
                     /locus_tag="ACGVJG_003090"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFJ6558972.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..>2201)
                     /locus_tag="ACGVJG_003091"
     CDS             complement(1877..>2201)
                     /locus_tag="ACGVJG_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6558973.1"
                     /translation="GNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALRHSH
                     VAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILSKND
                     NLLPV"
BASE COUNT          654 a          429 c          351 g          767 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc a
//
LOCUS       DAYLZO010000235         2128 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.249, whole genome shotgun sequence.
ACCESSION   DAYLZO010000235 DAYLZO010000000
VERSION     DAYLZO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.249"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..618)
                     /locus_tag="ACGVJG_003092"
     CDS             complement(<1..618)
                     /locus_tag="ACGVJG_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HFJ6558974.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKA"
     gene            741..1208
                     /locus_tag="ACGVJG_003093"
     CDS             741..1208
                     /locus_tag="ACGVJG_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFJ6558975.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(1471..1953)
                     /locus_tag="ACGVJG_003094"
     CDS             complement(1471..1953)
                     /locus_tag="ACGVJG_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFJ6558976.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2053..>2128)
                     /locus_tag="ACGVJG_003095"
     CDS             complement(2053..>2128)
                     /locus_tag="ACGVJG_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFJ6558977.1"
                     /translation="SFTKWGEDFSNVYSEIWIAVKAKK"
BASE COUNT          623 a          472 c          340 g          693 t
ORIGIN      
        1 tgccttggct aattcttcat agtcagttag attgccggat aattctaaga tttctggaga
       61 gtcactttct actagtactt ttgctgcagc ttctgcatat tcacgtttca atgcccaacc
      121 tgtttttcca tttcctgcag cataaacgaa ttgaccagtt tttaaagcct gcccaatgat
      181 aggtaattca ttctctacat accaattatt ccgtaaaaat gtatgggaaa tacctgaatc
      241 cttaatcatt ttttcagtga attggtgatc tggcgctaat acactgatcg agtgatcggc
      301 atctgcaaaa cttgtataag cgatataaga taccccagct gcttgtgctg ccttcactac
      361 attcttatgt tcggcttgac gatttcctgg aacgccagag acaaaaagca atcgatctat
      421 tcctttcaac gcttctttca ttgagtctaa atcatcataa tcaccaatac gtatattaaa
      481 tccagcttct ttcagtgctt ctcctttttc ttcactacgt actagtacat agatttcatt
      541 ttgtggaacc atctttttta agaattccag cgcataacca ccaaaacctc cagttgctcc
      601 tgttactaaa taatgcatat ttgccactcc ctattctttt tatttgttaa gatttacagc
      661 tgtacattta tatattacac ctttatcata aagagtgtgg taagataagt caagcaaaaa
      721 aaatgaaagg atgacaactg atgaagtatt ctctgcaatt tagtgatgca atccatgtac
      781 ttgcctatat tgaaatgttt caagataccc gatcactttc tagtgagatg attgccagta
      841 gtatcgaaac aaatgcagca aatgtacgta aaatcatgag tcagctcaaa aaatccggtt
      901 tgatctttac aaaaaccgga aaaccgcagc cttcccttgc caaaagtcca gatcagattt
      961 ctttactaga tgtctatcgc agcatcgaag gtaatacaca gcttatccaa gtcgatccaa
     1021 aaacaaatcc aaattgcgtc gttggtgcga atatccaaga agctttagaa gaaagctacg
     1081 ctcggctgca gaaaaaggca gaagaagaaa tggctaagat cacacttgat agtctgatac
     1141 ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa cacaggcctc gtagagagat
     1201 ttttataatt ctctctactg tgcctgcatt tttctgatcg gaatcgtcta cataaattct
     1261 tttattctcc gggggacaaa agccttgtca tgcccctttt tcctctctaa tgctcaggtc
     1321 aaagcgctga agtcatgacg tcttgataaa cggtgttcaa gtagctgacc ctcggcatta
     1381 gttctgaaaa atgcaaaata tggaaagcta tttcccattt ttcctctcta atgctcaggt
     1441 caaagcgcta ctttcacgac ctctatatta tcatggttta ttgactaccg gtatccagat
     1501 ttctcccata tatttcccgt tggtaactcc catttcgact acagcatttg gacccccaac
     1561 ataggcgtaa tcttctactt ctcttaaaac atgcccaaag gccgtattcg tcaaatcatt
     1621 tcttaattct tcaggcgatt ctgctaaagc gattacaaca agatacgttc cttttgggaa
     1681 tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt tgaacaccga catagtacat
     1741 cattttcccg ttatagggtt cgttgactgt aaagaaataa tgattttcag cagcttgttt
     1801 caatgcatct attgttccat taccgataac tttttgctca aatgcttgtt tctctgcagc
     1861 caaaccgaca aaatccgtat tgctactctt caattccaca ccaatacccg tcaccgtaaa
     1921 atctggtttt tcttcgatcc tatactttgc catttctccc accctttcta ttatattaag
     1981 ttcatcttac ataaaattaa agggaaatga cacgattacg ctcttttttc tagggatatt
     2041 tttatttttt aactattttt ttgctttcac tgcaatccaa atctcactgt aaacattact
     2101 gaaatcctct ccccattttg taaaagag
//
LOCUS       DAYLZO010000236         2029 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.250, whole genome shotgun sequence.
ACCESSION   DAYLZO010000236 DAYLZO010000000
VERSION     DAYLZO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.250"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..879
                     /gene="gltX"
                     /locus_tag="ACGVJG_003096"
     CDS             <1..879
                     /gene="gltX"
                     /locus_tag="ACGVJG_003096"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFJ6558978.1"
                     /translation="TYNFAVAVDDHLMKITHVLRGDDHIANTPKQLMIYEAFGWTPPT
                     FGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPDAMFNFIALLGWSPVGEDEIF
                     EHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMDLDAFTDMCLPYLIADGRVAE
                     NPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEHPVLNEAAKEVLAGETVPTVL
                     HAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFMPIRVAVSGQMHGPELPETIE
                     LLGREKSLAHLNQVLN"
     misc_binding    876..1115
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1201..1737
                     /gene="epsC"
                     /locus_tag="ACGVJG_003097"
     CDS             1201..1737
                     /gene="epsC"
                     /locus_tag="ACGVJG_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFJ6558979.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            1734..>2029
                     /locus_tag="ACGVJG_003098"
     CDS             1734..>2029
                     /locus_tag="ACGVJG_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HFJ6558980.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEE"
BASE COUNT          679 a          393 c          445 g          512 t
ORIGIN      
        1 acttataatt ttgctgttgc ggtcgatgat catttgatga aaatcactca cgttttacgt
       61 ggagatgacc atattgccaa tacaccaaaa caactgatga tctatgaagc atttggctgg
      121 acaccaccaa ccttcggaca tatgacattg atcatcaaca gtgaaacagg taaaaaacta
      181 agcaaacgtg acgaatcaat cttgcaattt atcgaacaat atcgcgaatt aggctatcta
      241 ccagatgcaa tgttcaactt tattgcatta ttgggctggt cacctgtggg cgaagacgaa
      301 atcttcgaac accaagaatt gatcaaaatg ttcgatccaa aacgcctaag taaatcaccc
      361 gcagctttcg atgcgaaaaa actgcaatgg gtaaacaacc attacatcaa agcaatggat
      421 ctagatgcat ttactgacat gtgtctgcct tatctaatcg ctgatggacg tgtagcagaa
      481 aacccagatg aaaagacaat cgaatgggtg aagaagatcg ttagcctgta tcaacctcaa
      541 atgagctatg ctgcagaaat cgttgatctg tctacgctat ttttcaatga acatcctgta
      601 ttgaatgaag cagcaaaaga agtacttgct ggtgaaacag ttcctactgt tctccacgca
      661 ttcaaagcac agctagaaca aatggaagta gtagatgctc caactgtcaa agctgccatc
      721 aaagctgttc aaaaagaaac tggcgtaaaa ggaaaaaatc ttttcatgcc aatccgtgta
      781 gctgtatcag gacaaatgca tggtccagaa ttgcctgaaa cgatcgaatt gcttggtcgt
      841 gaaaaatcat tagcacattt aaatcaagta ctgaactaaa cacgatgaac agaaaaaagt
      901 aagcagctga tcattacaag agagataccg ggtggtgcaa ggtatcaatg aatggttgcc
      961 gaaatgcctc tgggagtggt cttggcgaaa cacagaaaac aggaaaaact gttttatagg
     1021 aaacaaagta gccaagaacg tataacaggc gttagctgtc agagataatc attgattatg
     1081 aagaaaagtg gaaccacgcg gaaagcgtct tttgataagg aaacttattt aaaaggcgct
     1141 tcttttcttt ataagaagat gatttcctac ataaaattct tacgaaagga gtggaaagtc
     1201 atgagttggt ttacccgagc gattgatgca gcaaagagaa acgatccggc tgcacgttcg
     1261 acagctgaga tcgttttgac ataccctggt tttcatgcct tgttttggca tcgtttctct
     1321 cattttttat atcagcaccg gctcttttta ttggcgaaga tcaatgctca attctggcgt
     1381 ttcctaactg gtgttgagat ccatccagga gcaacgattg gaactggcgt gttcatcgac
     1441 catggaatgg gaatcgtcat tggcgaaacc gcagaaatcg aggacgatgt gattttattc
     1501 catggcgtaa cattaggcgg cacaggaaaa gaaacgggta aaagacaccc aactgttaaa
     1561 caaggagcca tgctctctgc gaatgcgcag atattaggac cggtaacgat cggaaaaaac
     1621 gccaaaatcg gtgcaggtgc cgttgtgttg aaagatgttc cagacgacgc tacagcggta
     1681 ggagtaccag caaaagtcgt tcgaatcaaa ggagaaaaag tgaggagatc agaatgataa
     1741 aaatctacaa tacattgacc agaaaaaaag aagaattcat ccctatacaa aagggacatg
     1801 tcggtatgta tgtctgcgga ccgactgtgt ataattatat ccatatcgga aatgctcgta
     1861 gcaccattgc tttcgataca attcgacgct attttgaata tcgaggctat aaagtcgatt
     1921 tcgtatcgaa tttcacggat gtagacgata aaatcatccg tacagcaaac gaattaggta
     1981 ttacagcacc agaagttgct gatcgtttca tccgagcatt tgaagaaga
//
LOCUS       DAYLZO010000237         2020 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.251, whole genome shotgun sequence.
ACCESSION   DAYLZO010000237 DAYLZO010000000
VERSION     DAYLZO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.251"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..563)
                     /locus_tag="ACGVJG_003099"
     CDS             complement(<1..563)
                     /locus_tag="ACGVJG_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFJ6558981.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACGVJG_003100"
     CDS             complement(750..1400)
                     /locus_tag="ACGVJG_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558982.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
BASE COUNT          704 a          401 c          246 g          669 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat
//
LOCUS       DAYLZO010000238         2002 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.252, whole genome shotgun sequence.
ACCESSION   DAYLZO010000238 DAYLZO010000000
VERSION     DAYLZO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.252"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..377
                     /locus_tag="ACGVJG_003101"
     CDS             <1..377
                     /locus_tag="ACGVJG_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFJ6558983.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            425..1825
                     /locus_tag="ACGVJG_003102"
     CDS             425..1825
                     /locus_tag="ACGVJG_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFJ6558984.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaatgtttc cacagaggac ttctttaatt attgtcgatt agaggaaagt ggtgtaacag
       61 tcactagcgg tacagattta ccgctgttta ttactgatat tcctgaatcg attataagag
      121 cagtattccg tcgttttcct gaaggagaag aagtctggga gaaagaaaca agcttcacga
      181 caaccgagct gctgaaaagt tttacaagca acagcttcaa agtaaatggt ttatcggagt
      241 acggagaaat aaaagtaggg aaaagcgcca gcttagctgt tttcagcgag gacctctgta
      301 ctgatgactg gcaaaaagtg atggctgcgg aagttatcgc tacctatctt gatggtgact
      361 tagtttacaa gaaataatgc ttttcgatat aacgttataa aacgaattaa agagaggtta
      421 aaaaatggaa acaatgaaaa gaaaattcac cttcccacat agttatacat tgatttttat
      481 cttaattatt ttggctgcaa ttttaacttg gataattcct agtggtcagt ttgagacagt
      541 tgttgaaaac gtcgcaggta tggaaaaaac aacggttgtt ccgggaacct atcaccaaat
      601 cgataaggca gattatggac aaggtttttc ctctatacta atggcaccag ctgaagggat
      661 tatttcagct gttgaggttg ttgcttttgt tctcatcgtt ggtggtgcct tcgggattat
      721 cctaaaaacg ggagccatcg atcgagggct gtttacctta gcaaaagttc tgggagacaa
      781 ggggatctta gttattccga tgtcgatgat tctattcagc ttaggaggat caacctttgg
      841 catgagcgag gaagtcattc ccttatttgc ggtgtttatt tctctgatgt tttctcttgg
      901 ctatgattcc atgactgcga tcttgattct atttttaagt acacaagcgg gatatgttgg
      961 gtcgacgatt aatcccttca acgttttaat cgcacaggga gtagctggcg tttacggaaa
     1021 tcccctgctt tggtttcgga tgctatcctg gattgtcatt actgggattt caattgtttt
     1081 caccatgctt tatgctagca gagtcaaaaa gaatccactg tcctctattg tttatgagag
     1141 tgatttaaag gttaagcata agtttcaaaa ttttgacact gaaaaagagt tttcaacaca
     1201 agataaacta attatcgccg gttttatcgt aggtttagga atcatgattt ggggtatcat
     1261 ttccaaggga tggtatatgg ttgaaatttc tgcactattc ttaggattag gtctttttgc
     1321 tgggggcatc gctagatttt ctcaaaagga aattgctgaa gcttttgtcg aaggctgttc
     1381 agatttcgcc tatgctgctg taatcattgg gctagctaga ggagtcttgg taatcttgga
     1441 aggtggaatg attattgaca ctattctaaa cagcttggct aaccttttag gtggtcttcc
     1501 aaaattcctg ttcactacga ttctactcat ttctcaagta attgtttcct tttttgttcc
     1561 atcctcctca ggagctgcgg cattaacgat gccggttatg gcaccattgg gtgatctaat
     1621 gaatgtaagt cgtgatgttg ttgttctaag taaccagttt ggaaatggtt taatgaatat
     1681 tatctcccca actagtggtg tattactggc aggattagcc atcgcagaga ttaatttctc
     1741 taaatggttt aaggttggct tcaaaatatt tgccatctta acccttgttt ctgctgtttt
     1801 gttatacatt gctacactct tttagtctta atcatccatt aataggaggc aatagttatg
     1861 attaaaataa atcaattagc aggaatgaat atccactatt tattttattc tctcaaagaa
     1921 tttttcgctt atcaacagaa aattggtatt tcttctgttg aactttgggg aggtgctccg
     1981 cattttttca tagatgcttt ta
//
LOCUS       DAYLZO010000239         1987 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.253, whole genome shotgun sequence.
ACCESSION   DAYLZO010000239 DAYLZO010000000
VERSION     DAYLZO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.253"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..393
                     /locus_tag="ACGVJG_003103"
     CDS             <1..393
                     /locus_tag="ACGVJG_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558985.1"
                     /translation="LKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVK
                     ELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAA
                     TESDDICKKVCNGEENYWIGEEKLNEIY"
     gene            380..832
                     /locus_tag="ACGVJG_003104"
     CDS             380..832
                     /locus_tag="ACGVJG_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558986.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            825..1223
                     /locus_tag="ACGVJG_003105"
     CDS             825..1223
                     /locus_tag="ACGVJG_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558987.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            1535..1608
                     /locus_tag="ACGVJG_003106"
     tRNA            1535..1608
                     /locus_tag="ACGVJG_003106"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1569..1571,aa:Ile,seq:cat)
BASE COUNT          722 a          268 c          353 g          644 t
ORIGIN      
        1 ttaaaatatc ttgaaattta ctctgttaaa aaaaataaat taattggtga aaatgattct
       61 ccaataaaaa cacctattga gctaagctta agaaatcgga tgggaatact ccaaagatcg
      121 gaaatagtaa aagaactttt ttatcataaa tttagttatg aaataagact agatgatact
      181 tatgagcatt atagaattgt ttttttcgta tacaattcta tagaagatgc tactgcgact
      241 actgcattaa cattcggttt tacgaaaaat ggaacaatca attcggataa aacccgtcaa
      301 gctgctacag aaagtgatga tatatgtaag aaagtatgca atggggaaga gaattactgg
      361 ataggggagg aaaagttgaa tgaaatctat taatgattta ttaaaaaaac atcctgattt
      421 atataaaaaa ttaaattctg aacaaagaaa cctgtccaaa caattactta aaataagatt
      481 aaaaaaacaa aaaaacgtga cagaattctc aaaaatgtta ggaataagtg aaaaaaaata
      541 tcttgaattt gagttcggag atatgcatat tcctgttgca gagtacaata aacttttgaa
      601 acaagttgaa aatttgtcct ataaagattc aatgaatttc actattaagg ttgctggcgg
      661 ccctattaaa tatgaccgta ttgaaaataa accttatatt cggaataaaa gttactcagt
      721 atttaaaagt agaaagagaa aagaagtatc gtcaaatatg gtctatgcta gtgttaatta
      781 ccaagatacg agccgctata ttgtagaaaa agaggtatat gtacatggat aaaggaaata
      841 gtgttacttt tgcacttgaa aatattagtt tgaataaaat tcattttgaa ttaactgata
      901 attttagtta tggagattta gagtttaata ttgaacttgg atataaagaa gacgaagcag
      961 agtatctaga aaaaaaactt gtttctttac gagctcacat tgtagggggg atggaaggag
     1021 caccggtaga tattttactc ttttcagtta ttcgtgtttc tacaaatgaa cagccatata
     1081 aaaaattagt agaagaatct atgggagagc tagctaaacc tatcctgaat aaagcttcta
     1141 atttgatttc taatttatat agcgaagtag gagttatgcc tacagttatt gatttatatc
     1201 agctatatag tcaacaaaaa taaaatacaa gtaatagagt tatctattta aatttgcaag
     1261 acaaagacct caaccagtca tccttggttg aggctttttt gctctgtaat taactcgtat
     1321 agatcctgca agatagtttg tttctgaata aatgattgat atgaagtatt tagatgactg
     1381 tattgacaac tataaaaatc attcgataaa atagtgatgt tatcgcatat cttcactatc
     1441 acccataata gtcacactcc aagctatgcg ataacaggat tgttgccaca ccttctactg
     1501 gttgattgtt tatggcttta tgtggcaaca acctgtactc ttagctcagt tggtcagagc
     1561 agacggctca taaccgtccg gtcgtaggtt cgagtcctac agggtacata atttaatcta
     1621 tttgataaaa aataaaattc aataatggta taataaaatg tcggctcgtc gcttccacag
     1681 agattttcgt gggaggaggt gtatgatttg tttgaattca ctttggagca cgtgctcttg
     1741 ccattgttaa ttggtttaac tttggcatta tttagtcatt ggctagataa acattagagc
     1801 cgacaatggc gcttactgtt tcgcagcggt aagcgtcttt ttttgaaaaa gaaaaaaaca
     1861 caacgtattc gcaggtacgt tgtgtttatg gtgtatattt gtttaaattc actttcaatt
     1921 attattatac ttgttaaata aattgtgtca atataaaaga tatttttata cgtgtactaa
     1981 cctatat
//
LOCUS       DAYLZO010000240         1980 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.254, whole genome shotgun sequence.
ACCESSION   DAYLZO010000240 DAYLZO010000000
VERSION     DAYLZO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.254"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(729..1745)
                     /locus_tag="ACGVJG_003107"
     CDS             complement(729..1745)
                     /locus_tag="ACGVJG_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFJ6558988.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          558 a          459 c          309 g          654 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaaa attttcctaa ttgtgacatg gctgtcacaa
      541 ttccaaatgc tctccgaggg tattcgctgt cacaagtgac aacgaaaaaa ggagccgaaa
      601 gcgtgtcacg ctctcaactc cttttacgct ttattttgct tcatgcaaaa tattcgctta
      661 tcgtgaattt acgtcactaa acattgacgc aaatccactc tcactcatct gacttttctt
      721 attcggtatc acttctctgt cacatccaac cccaaaagcc acgtatccaa ttcttttttt
      781 aattcgtctg cagattcctt aaacgtctct cgtaaatgat tcgttaactc atcattttga
      841 ctttcttgct tcagcaaatt ccgatattta tattttgtat gtctagcaat tatcgaccaa
      901 ttagaaggct cagctatcaa accatcaata actattttat caataggttt tatatcaatc
      961 ggcaaaaaat gcgggcttgc aaaactatct aagctttcct gaaccatcgc atgccaatcc
     1021 gttgctttgc cacgtcttag ctgcatttct aaccgccacc acgtatcgat ttcttttggc
     1081 acgattactt tttttcgttc ctgctctagc tttttgttgt acagtcgcac ttggcgctca
     1141 ctggcacgac tgccccagta cgctgtttct agcttgccag aacggccgta gattggctta
     1201 aaagaaatcg ggtctacaat acgatactga gtaataaagt catccggcac atctacaata
     1261 tcgcaggcaa tatccgcccg ggaaaaatgc ggatctaaaa ataggtcatg aatgaatcct
     1321 ttcatggaac tagctaaaaa ctggcctatc ttattcggat taaaatcaat acgacctttt
     1381 ccttcatgaa atttaagtat ttcgatatac gcaatatctt ctccatattg atcgaataat
     1441 tgccagccgt ttatcccaac tgatttcaaa tatccctgat tttcgttcaa acgcattaat
     1501 tgctcgaaat cgacaaaaac aactccattt ttcgtccaat aagctatggt atcattcagt
     1561 tttccgacaa ttgtaattcg atcaatcgac caatttaatt ttaggggggt ggtcgtacct
     1621 tgcacactta accgcctgtt agaaatacgg ttaagttttt cgagctgatt tatcgttgtt
     1681 cttttttctg cccctcgacc ttcaattttt gattgaattt cactcaactg attttcattt
     1741 gccattttcg tcctccaaac gaaaaaagag acttttggaa agaatttcca gagttcacgt
     1801 aaccctaaaa attcatatcc aaaaatctct agcttgaaga aacctaaaaa tattcgtata
     1861 atgaacctga gttacggagc taggtgaagt agtttccgta aatagtcgag agtgttagcg
     1921 cactgctcgg cttttatttt gctctttttt ctcgttttct ttgattatag cactcctcct
//
LOCUS       DAYLZO010000241         1973 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.255, whole genome shotgun sequence.
ACCESSION   DAYLZO010000241 DAYLZO010000000
VERSION     DAYLZO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.255"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..489
                     /locus_tag="ACGVJG_003108"
     CDS             <1..489
                     /locus_tag="ACGVJG_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HFJ6558989.1"
                     /translation="GFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFL
                     FSHPAVEKVYYPGLPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAE
                     SLGGVESLVEIPSVMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRI
                     KN"
     gene            660..1118
                     /locus_tag="ACGVJG_003109"
     CDS             660..1118
                     /locus_tag="ACGVJG_003109"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFJ6558990.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1277..1747
                     /locus_tag="ACGVJG_003110"
     CDS             1277..1747
                     /locus_tag="ACGVJG_003110"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFJ6558991.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(1800..1915)
                     /gene="rrf"
                     /locus_tag="ACGVJG_003111"
     rRNA            complement(1800..1915)
                     /gene="rrf"
                     /locus_tag="ACGVJG_003111"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          664 a          367 c          371 g          571 t
ORIGIN      
        1 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
       61 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      121 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      181 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      241 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      301 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
      361 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
      421 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
      481 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
      541 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
      601 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
      661 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
      721 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
      781 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
      841 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
      901 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
      961 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1021 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1081 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1141 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1201 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1261 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1321 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     1381 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     1441 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     1501 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     1561 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     1621 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     1681 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     1741 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctttg
     1801 cgtggcgacg tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct
     1861 taacttctgt gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg
     1921 gtgttatctt ttattgagta aattttgttc actcaaaact ggatttgaag tca
//
LOCUS       DAYLZO010000242         1944 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.256, whole genome shotgun sequence.
ACCESSION   DAYLZO010000242 DAYLZO010000000
VERSION     DAYLZO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.256"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..965
                     /locus_tag="ACGVJG_003112"
     CDS             <1..965
                     /locus_tag="ACGVJG_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6558992.1"
                     /translation="VTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKGHAS
                     QSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISLYDE
                     DQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEMLSE
                     KLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINISER
                     VRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMENVIF
                     PLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            1416..>1944
                     /locus_tag="ACGVJG_003113"
     CDS             1416..>1944
                     /locus_tag="ACGVJG_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFJ6558993.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVV"
BASE COUNT          673 a          325 c          385 g          561 t
ORIGIN      
        1 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
       61 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
      121 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
      181 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
      241 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
      301 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
      361 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
      421 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
      481 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
      541 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
      601 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
      661 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
      721 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
      781 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
      841 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
      901 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
      961 aataaaaaca gaggttgtga aatcagcggt ttgacctgag tattagagaa acaaacggca
     1021 aaatagtttt tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1081 ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag caaaaagaat actcaaataa
     1141 aattagctcc tcatgtttta catttgagaa gactgattgc cgtagataaa agcttttgaa
     1201 caccatttat agaagaaaca agaatatttt gctattttaa acaagaggat agacaaatac
     1261 atgtctgatc tcttgttttt tattgggcaa cagacctctt agaatgtata aagataaatt
     1321 ttacaaacgc tttcggttca gttataataa taaatgataa cttgtatata taagttgtgg
     1381 taggtaataa atcagttgaa tggaggtaaa tatccatgac atttaaagac aaagtcattt
     1441 accaaatcta tcccaaatct tacaaagaca gtaatggtga tggaattgga gatttaaaag
     1501 gaatcaaaga aaaactcccg tatctgaatg atctaggcat cgacatgatt tggctaaacc
     1561 ctatttatcc aagccctcaa aaagacaacg gttatgatat ctctgattac acagccatcg
     1621 atcctatctt tggtacgatg gaggaattcg aacagctaat agccgaagca aagaaatacc
     1681 ggatagaaat catgctcgat atggtgctga atcacgtttc gacagaacat gaatggttca
     1741 aaaaagcact agcaggggac cagcattatc aggacttctt cattttaaga gaagagccaa
     1801 cggattgggt atcaaaattt ggtggaagtg cttggtcgcc atttggagat acggggaaat
     1861 attatttgca cttatatgat cggacacaag ctgacttgaa ctggcggaac caagcagtaa
     1921 gagaggaact attcaaagtt gttc
//
LOCUS       DAYLZO010000243         1930 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.257, whole genome shotgun sequence.
ACCESSION   DAYLZO010000243 DAYLZO010000000
VERSION     DAYLZO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.257"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..609
                     /locus_tag="ACGVJG_003114"
     CDS             <1..609
                     /locus_tag="ACGVJG_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6558994.1"
                     /translation="EGTVASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVK
                     NRMGEKTIGFGTQNQKLSEDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDD
                     LVRILTEPKNALVKQYQKLLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEI
                     MMDVMFDIPSNENIEKVIITKEAAELSGEPTVIYKDSEKKAG"
     gene            692..1279
                     /gene="yihA"
                     /locus_tag="ACGVJG_003115"
     CDS             692..1279
                     /gene="yihA"
                     /locus_tag="ACGVJG_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFJ6558995.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(1353..1508)
                     /locus_tag="ACGVJG_003116"
     CDS             complement(1353..1508)
                     /locus_tag="ACGVJG_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFJ6558996.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            1644..>1930
                     /locus_tag="ACGVJG_003117"
     CDS             1644..>1930
                     /locus_tag="ACGVJG_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6558997.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLS"
BASE COUNT          642 a          330 c          394 g          564 t
ORIGIN      
        1 gaaggaactg ttgcaagcgt gccacctcag ggaggacgta aacatccaca tcaagaattt
       61 attcagattg atacgacgaa catattgttc atcgtaggtg gtgcatttga tggcatcgaa
      121 acgattgtga agaatcgtat gggcgaaaag acgattggat ttggtacaca aaaccaaaaa
      181 ctttctgaag acgaaagtgt catgcagcat atcattcctg aagatttact aaaattcgga
      241 ttgatccctg agtttatcgg acgattacct gttatggcag ctttagagaa attgacaaca
      301 gatgatctgg tacggatctt gactgagccg aaaaatgcgc tagtcaaaca atatcaaaaa
      361 cttctttctc tagataatac cgaacttgaa ttcgaacctg aagcgttgag agccattgcg
      421 aaaaaagcaa tcgaacgcaa taccggtgct cgaggacttc gttccatcat tgaagaaatc
      481 atgatggatg tgatgtttga tatcccttca aatgaaaaca tcgaaaaagt gattatcaca
      541 aaagaagcag ctgaattgtc tggtgagcca acagtaatct ataaagattc agagaaaaaa
      601 gcgggttgat gaaaagaggc tgccgtaaaa gtcattttgg cttttgcgac agccgcttgc
      661 tttattcatc accttgttaa ggagagagac catgaaagtc catcaagctg aaattatcat
      721 aagtgcagtg gcaccaaaac aatatccaga tacacaattg ccagaaatag ctctggcagg
      781 acgatcaaat gtagggaaat cttcttttat caacacattg attgatcgaa agaatttagc
      841 ccgtacttcc ggtaagccag gaaagaccca gacattgaat ttttatttga tcgagaacgc
      901 attacatttt gtcgatgttc cgggatatgg gtatgcaaaa gtatccaaaa ctgagcgagc
      961 aaaatgggga aagatgatcg agacatattt aacgactaga gaccagctac gtgcggtcgt
     1021 ttctttagta gatttgcgtc ataagccttc tcaagaagat atccagatgt atcaattttt
     1081 gaagtattat gatatccctg taattgttgt tgccactaag gcagataaga tccctcgtgg
     1141 taaatggaac aagcatgaga gcgaaatcaa aaaagcgttg gattttgatc gcaatgacac
     1201 gtttattctc ttttcttccg ttacaaaaca gggaaaagaa gaagcgtggg cagcaatcga
     1261 acaagcaatt gaatcataaa aagaataaaa aagacttacc agtcaaactc tttcataaaa
     1321 gtttggctgg taagtctttt tttgagctta acttactttt gtttcttgtc ttttttcttt
     1381 tcattttgtg gctgtggttt tagaaatcga tcgatctctt cttttggtgc aactgttttt
     1441 tccggatcga cagcaaatga ttcgaaaagt ttatctaaat tatctgtacg ttcaaatttc
     1501 aaccccattg taaacacctc tttgtttctt tttacttagt ctatcatgta atcgtccaaa
     1561 ttggtttcta agacataaag ttttagtttt tctttatcgt tggttataat taaattataa
     1621 aaagatttta aagaggtgag aaaatgattg gcgaagataa attattgatg actatccagc
     1681 tcacagactt aaatgaagaa gaaaaaaaac aggaaataag cgattgggct attttgacac
     1741 gtctgttgat ccagccaaca tttatccgct ctttcacaca acaagcggat ccgtatgatc
     1801 ttcgaaagct tcaagaaaaa ataatgctag catcggtaag agacgaaagt tggttagttg
     1861 taggaaatga cgagaacgaa tgtagttttc gcttggaaga taatcaatta ttgataaaaa
     1921 atgtattgtc
//
LOCUS       DAYLZO010000244         1917 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.258, whole genome shotgun sequence.
ACCESSION   DAYLZO010000244 DAYLZO010000000
VERSION     DAYLZO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.258"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..611)
                     /locus_tag="ACGVJG_003118"
     CDS             complement(<1..611)
                     /locus_tag="ACGVJG_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFJ6558998.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYS"
     gene            complement(953..1825)
                     /locus_tag="ACGVJG_003119"
     CDS             complement(953..1825)
                     /locus_tag="ACGVJG_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFJ6558999.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT          564 a          369 c          347 g          637 t
ORIGIN      
        1 cttgaataat tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact
       61 aaatggatga tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga
      121 ccgcttcgtc cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga
      181 atcccaagct cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg
      241 taaagcactt cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc
      301 tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc
      361 atcaaaacag tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca
      421 atctgattaa gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt
      481 tcgacatcac gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca
      541 atagctactt tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt
      601 ttgattttca tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg
      661 attgcggcca aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga
      721 tttttcatgc ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt
      781 tatatagaca gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt
      841 atcagaaata aaaggaagga acaaattaag aaacttatgt tatttaatca ttggatcttt
      901 tgcttaactt attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt
      961 atttctccgt taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt
     1021 tccaaatgag attcagtgaa gcttgtaatg gcggatcgag tgggatgaat actttttccg
     1081 tcccataagt attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca
     1141 tcaaagaagc attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga
     1201 aacctagcca ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa
     1261 cagttttcag ttcctgcgga tggatgactg tttgctttgc taaaggatga ttccgatgta
     1321 tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg
     1381 tcggttcgat tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat
     1441 ctgcattccc gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa
     1501 ggctatcaga aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag
     1561 agatgattgc gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt
     1621 ttccttttga ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagcctcgag
     1681 tgaaaagtgt cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt
     1741 gcgtgatatg cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa
     1801 aatacgtcag tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta
     1861 agtatagtta aaaggaatag ttaagtatgt tttataagta ttagacatca taagccg
//
LOCUS       DAYLZO010000245         1913 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.259, whole genome shotgun sequence.
ACCESSION   DAYLZO010000245 DAYLZO010000000
VERSION     DAYLZO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.259"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1009
                     /locus_tag="ACGVJG_003120"
     CDS             <1..1009
                     /locus_tag="ACGVJG_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFJ6559000.1"
                     /translation="FERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLANIG
                     GKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARIENL
                     EEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLMTLH
                     AAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNAFSR
                     TLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVPSKS
                     ATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAPIEK
                     A"
     gene            1026..>1913
                     /gene="ligA"
                     /locus_tag="ACGVJG_003121"
     CDS             1026..>1913
                     /gene="ligA"
                     /locus_tag="ACGVJG_003121"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFJ6559001.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSL"
BASE COUNT          651 a          353 c          446 g          463 t
ORIGIN      
        1 ttttgagcgt atcgttaatt cgcccaaacg aggcatcgga ccaggatcta tcgaaaaact
       61 acgcagtttt gcttctcttc atgaatggcc actgcttgaa gcagcgcaaa atgtggatct
      121 ggcaaatatc ggtggaaaag caggacagca attaggtgca tttggagaga tgatccaaga
      181 agtcactcag atgatcccat acttgacagt gacagaattg acgaaagagg ttttggatcg
      241 tagcggatat ctagaagact taaaaatcca aaatacatta gaagcacaag cacgcatcga
      301 aaacttggag gaatttttaa cggtcacaca agaattcgac aaacaattcg aacagcaaaa
      361 tgaagaagac gctgatgcac cagaagaaaa actaacggtc ttcttaaatg atctggcact
      421 tgtttccgat attgacaatt tagaagaaga tgcttcccaa gtgacattga tgactttgca
      481 tgcagccaaa ggattagaat tcccagttgt attcctgatt ggtctagaag agggggtttt
      541 tcctttatct cgagcattga tggaagaaag cgaactggaa gaagaacgcc gtctcgcata
      601 tgtggggatc acgcgagcag aagaagcgtt gtatttaaca aatgctttct ccagaacatt
      661 atatggacgg acgcaatata accgaccttc tcgttttgta gaagaaatcg atcaggagtt
      721 actggaaatc gaaggcatgc gtcctacgcc taaaaaaaca cctgtattcg ctaaaaaaac
      781 agcatatagc tataaacagc cagagacagc ggttgttccc tctaaaagtg ctactggagg
      841 ggaaaataac agctggaaac ctggagataa agtcaaacac aaaaaatggg gtctaggaac
      901 agtcgtccga gtaagcggga cgtcgaagga tctagaacta gatgtggctt tcccaagtca
      961 aggagtaaaa cgtcttttgg ctgcctttgc gccaattgaa aaagcttagt agtggagggt
     1021 aaatgatgga acatgaaatg acgattgacg aagctgctaa acgagcagaa gaacttcgta
     1081 caagactgaa ccaatggtct cgcgaatact acgtagaaga taaaccaaca gtggaagact
     1141 atgtttatga taaagaatac gcagaattag tagcaattga ggaacaatat cctgatttga
     1201 tcacgtctga ttctcccact caacgagtag gcggaaaagt attagaaggt tttgaaaaag
     1261 tcactcatga tattccgctt tacagtttga acgacgtttt cagcaaagaa gagctgatag
     1321 cttttgatca acgtgtacaa aaagcagtgg gacgtgtagt tgactattgc tgtgaactaa
     1381 aaatagatgg actttccgtt tctttgcgtt atgaagatgg aaatttcgtg agaggtgcga
     1441 ctcgcgggga tggaactgta ggagaaaaca tcactgaaaa cctaaaaact gtccggtcgg
     1501 tcccaatcaa attaaaagaa ccaatgaaca tcgaagtaag aggcgaatgt ttcatgccaa
     1561 aacggtcatt tgtccaattg aaccaagaca gagaagcaga gggaaaagat atctttgcga
     1621 atcctcggaa cgccgcagct ggttctttgc gtcagttaga ttctaaaatc acagcgaaaa
     1681 gaaatctgga cactttttta tacacagtag ctgatttcgg tcctatgcaa gcaaaaaccc
     1741 agtatgatgc gctagaagag ctagaaaaaa ttggattcca cacgaatcgg gaaaaacgtc
     1801 tatgtcattc gattgatgaa gtgtggtcat acattgaaga atatcatgac aaacgtgtgg
     1861 atttgcccta tgaaatcgat ggaatcgtca tcaaagtgaa tgaattctcg cta
//
LOCUS       DAYLZO010000246         1863 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.260, whole genome shotgun sequence.
ACCESSION   DAYLZO010000246 DAYLZO010000000
VERSION     DAYLZO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.260"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            173..574
                     /gene="tnpA"
                     /locus_tag="ACGVJG_003122"
     CDS             173..574
                     /gene="tnpA"
                     /locus_tag="ACGVJG_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFJ6559002.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            586..1731
                     /gene="tnpB"
                     /locus_tag="ACGVJG_003123"
     CDS             586..1731
                     /gene="tnpB"
                     /locus_tag="ACGVJG_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFJ6559003.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
BASE COUNT          698 a          287 c          324 g          554 t
ORIGIN      
        1 gactagccga gaacacccgt gattttaatc atgggatgaa tcggattcag ttgtgaccta
       61 gctttagcta ggttaaacaa ctgacaaata taaactaaat aatagtgaaa tgtcttgcaa
      121 taattgattt tgtagaaata catatattaa aataattgcg aggtgttttt gtatgtcaga
      181 aattcattat ggacggggct atgtctattc tattcaatat catcttgttt ggtgtgtaaa
      241 atatcgacat gatgttttgt atggtcaggt agatattgat gtcaaacagt ttttgaaaca
      301 aattgctgat gataataata taaaaattat agaaatagaa tcagataaag accatattca
      361 tttactcatt gaatgcaccc cacaacacta tattcctagt attgttaaag tatttaaagg
      421 cgcatccgct agactgctgt ttaaaaaaca tcctgaactt aaacaactcc tttggggtgg
      481 acatctttgg aatccaagct actttgtagc tactgttagc gaaaatacag aagaacaaat
      541 tagaaaatat atacaaaatc agaaaaagaa atgaggtgaa tccgtatgct acaacatagg
      601 gcttataaat ttagaatcta tcctaatcaa gaacaagaaa tattaatgac taaaactatt
      661 ggctgttcaa gatttgtcta taattatttt ttgaatttgt ggaatcatga atatacgact
      721 actggaaaag gattatctta taattcttgt tctgccatgc ttccacaaat gaaaagaaat
      781 gcagagacaa tttggttaaa agaagttgac agtattgcga ttcaatcctc attgaaaaat
      841 ctttctgatg ctttttctcg cttctttaaa aagcaaaata aacgaccaca atttaaaagt
      901 aaaaaacatc ctatacaaag ttatacaact aaaaatgtga ataatagtat cgaaattaga
      961 aacaatttcg taaaactacc taagttagga tttgttaaat ttgcgaaaag tcaagagccc
     1021 aaagggcgaa tcttaaatgc tacgattcga aaaaattcga gtggtaaatt ttttgtttcc
     1081 atcctgtgtg aagaagaaat agttgagttt cctaaatcta attccgctat tggaatcgac
     1141 cttggtataa ctgattttgc aatcctttct gatggtcaaa agattgataa caataaattc
     1201 acgtcaaaaa tggcaaaaaa actcaaacgt gaacaacgta agttgtcaaa acgtgcatta
     1261 ctagctaaaa agaaaggcgt caatctcttt gaagctaaaa attatcaaaa acaaaaacga
     1321 aaagtagcac gactacatga aaaagtaatg aatcaacgca atgacttttt aaacaagttg
     1381 agcacagaga ttatcaaaaa ccacgatatt atctgtatag aagacttaaa tacaaaaggt
     1441 atgttgcgta atcataaact agcaaaaagc atttctgatg tgtcttggtc tagttttgtg
     1501 actaagttac aatacaaagc taactggtac ggtcggaaaa tcatcaaagt agataaatgg
     1561 tttccatcta gtcaaatctg ttcaaattgt ggacacaaag acggcaagaa atctctagaa
     1621 attcgagaat ggacttgtcc tgtttgtcat gctcatcatg accgagatat caatgctagt
     1681 atcaatattt tgaccgaagg tctaagatta catgtcatgg gattagctta gatccaatca
     1741 tcaaaaaccg taggaactac ggggatagct tggtaaataa gagaaaccgc tgtgagtaaa
     1801 gaaatacgct tgcaagtatg ctctattccc aagaatctcg tgactttagt catgagaggt
     1861 tca
//
LOCUS       DAYLZO010000247         1849 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.262, whole genome shotgun sequence.
ACCESSION   DAYLZO010000247 DAYLZO010000000
VERSION     DAYLZO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.262"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            88..723
                     /locus_tag="ACGVJG_003124"
     CDS             88..723
                     /locus_tag="ACGVJG_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFJ6559004.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            772..1452
                     /gene="cmk"
                     /locus_tag="ACGVJG_003125"
     CDS             772..1452
                     /gene="cmk"
                     /locus_tag="ACGVJG_003125"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFJ6559005.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            1587..>1849
                     /locus_tag="ACGVJG_003126"
     CDS             1587..>1849
                     /locus_tag="ACGVJG_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HFJ6559006.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIG"
BASE COUNT          663 a          319 c          402 g          465 t
ORIGIN      
        1 taaaaaaaac atggaacatt gattttttag gacaagacac tttttcctat ggtataatac
       61 acacgataga gtttaggagg taaaacagtg agcagaaaag atagacatca atctacagaa
      121 aataacgaaa cacaagaacc atgggaacaa ccaatctacg atacagatga tgaagtttca
      181 tcaagaagtt ctcagagaca acaaaagaaa ggaaatacat tatttctttc gctatttttg
      241 attttactgg tcttatgtat cgctattcca gcaggtgctt atttctggat cagaaatgga
      301 tcaagcaaca atgcaacagc agcaacttct tctgaaactt cgacatcagt agtagaaagt
      361 tctacaagta caacaaaaga ggagtcaact gagacttcaa cagtagaatc tagtacaatc
      421 gcggaagaat ctactgtgtc tcaagataca ggtgtaacgt cagaaagcag tattgcagca
      481 gagacaacac cgacttcctc aagtgaggca acttatcaag caggggattc aagcagcaat
      541 gcagcagaaa caacaccttc ttcatcagct gcagccggtt caacaacgac tgttcaagca
      601 ggcgaaggtc caaaacaagt ggctgagcgt gcagggatca ccactgatca attgtttgaa
      661 ttgaatggct tagatccaaa caactttatg ctttaccctg gtcaagaatt aagaattaaa
      721 taataatagc cgcgctttgc ggcttttttt ctgagaaaaa ggagttatat catgacacaa
      781 atcagtattg caatcgacgg gcctgcttca tctgggaaaa gtaccgttgc caagatatta
      841 gcaaaagaat tgaattatat ttatacagat accggtgcca tgtatcgcgc agtaacttat
      901 ttagctatta cgaacaatgt atctttttca gatgaagaag ggctagtttc gctgatcaaa
      961 aagtatccta tctctttttc gcaacaagaa gatggacagc atgtattcat agcatcaaaa
     1021 gatgtgactt tagaaatccg tcaaccagat gtcacaaaag ctgtttctga agtctctgca
     1081 catggaaaag tcagagaaga attagttgct gtccaacgaa aaatagggga acgcggtggc
     1141 gttgtcatgg acggaagaga catcgggaca gctgtattac cgaatgcaga agtgaaaatc
     1201 tttttagtag caagcgtaag tgagcgtgca gaaagacgtt tcaaagagaa ccaagaaaaa
     1261 ggaattccga ctgatcttga aacactgacg aaagagatac aggaaagaga ccattatgat
     1321 tcaacaaggg acgtctctcc gctgaagcaa gcagaggatg ctgttcgtat cgatacgact
     1381 ggcaaatcga ttccagaagt cgttgcagca ataaaagctg ttgttttgaa aaaaggttat
     1441 cttctttttt aaattaagaa aaaaaatgaa aaacaattgt tttttcaagg aaaacgaacg
     1501 aaatcgcttt attttttttg aataatcaat taaactaaaa acagtaccgt acttacggca
     1561 gaatgttggt taggaggaca agtgttatga cagaatttga tcaaaatcaa gagaacaacc
     1621 agtcaatgga agacgcaatg aaaagcgtac aagaagtaaa agttggcgat gttgttaagg
     1681 gcgaagtttt agtcattgaa gacaaacaag tgattgtcgg aatcgaaggg gctggagttg
     1741 aaggagtcgt tccggcaaaa gaattatcaa ctttgccggt agaagatatc agcgaagtag
     1801 taaaagtcgg tgatgtatta gatttagtcg ttatcagtac gatcggcaa
//
LOCUS       DAYLZO010000248         1837 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.263, whole genome shotgun sequence.
ACCESSION   DAYLZO010000248 DAYLZO010000000
VERSION     DAYLZO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.263"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..164)
                     /locus_tag="ACGVJG_003127"
     CDS             complement(<1..164)
                     /locus_tag="ACGVJG_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFJ6559007.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELA"
     gene            complement(182..502)
                     /locus_tag="ACGVJG_003128"
     CDS             complement(182..502)
                     /locus_tag="ACGVJG_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6559008.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            801..1577
                     /locus_tag="ACGVJG_003129"
     CDS             801..1577
                     /locus_tag="ACGVJG_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFJ6559009.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIVADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1592..1795
                     /locus_tag="ACGVJG_003130"
     CDS             1592..1795
                     /locus_tag="ACGVJG_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6559010.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
BASE COUNT          690 a          306 c          297 g          544 t
ORIGIN      
        1 gcaagttcat gagataaatg aaaggcttct gcagtttcac cgtatagatt ttcattcaaa
       61 aaaatgattc tgggttttgg atagtagaaa ccaggctctt tcatctccat ataaactact
      121 ttcaaattgt attcgctcaa catttctttc aatttcaaat acatacaaac catcactcca
      181 actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt gtctccttag
      241 atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc tgataaatcc acatgtttag
      301 gggtttctct ttcttctcta cctagaaggt agtctacaga gacatcgaaa tagtcagcaa
      361 tccgacttaa ttcatctgaa ttaggagtat tatttttcca ctttgctaga taaccgttag
      421 aatatccaaa attaatttct aactgccgta tagatatctt tttcctcttc gccaagtctt
      481 ttattatttc ataggtattc atcgataaaa caacctttct aaatgtttac aaaaaaagtt
      541 tagaaaaata cgcagaaata atttgactaa ttctgagtaa tgagctatac tatatcttgt
      601 aaacagattt aacaactaaa aagacaacaa aaaacactat tgatatgtaa atgcagaccg
      661 ccaagaaagc tttaaaatca atgtttttat gtcttattta attatgctct tagtatagag
      721 tattactcag atactgtcaa ttgaatttag aaaaaagttg ttaaatttgt ttacgaatat
      781 aaaagaaagg aatgagaaat atgaacacac cgcaaatttt taatttcgaa caaaacgaag
      841 ttcggacaat tttagtaaat gatgaaccat attttgtagg caaagacgtt gcgagtgttt
      901 tgggttattc aaacactaaa gatgctttgt cgcggcatgt agatttggaa gataagatgg
      961 ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac aatcatcaac gaatctggtt
     1021 tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa aaaatttaaa cgttgggtaa
     1081 caagtgaagt gttgccagca attagaaaac atggaggtta tctaactcca gaaaaagtag
     1141 aagaagcttt gcttaatcca gatacaatca ttcaattagc aactcaacta aaagaagaaa
     1201 gaactggaag attaatcgca gaacaaaaga ttgcagagta cgaacctaaa atctcctatt
     1261 tagatagcat attatcttct acagattcag taacaattag tcagattgta gcagattatg
     1321 ggatgtctcc acaacagatg aataaattac ttcataaact aggtattcag aaaaaagtcg
     1381 gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg atacacaaaa tctcatacaa
     1441 ctgagatccc gaaagccgat ggtggcacta aaattgtaat gaataccaaa tggacacaga
     1501 aagggcgtct atttatctac gaattactaa aaaaagaagg atattaccct caaatggatt
     1561 tagaggaaat tggttagaaa ggagttttag tatgactgac attgcagaaa tcactcaacg
     1621 agatagagaa aaaatcaaag aatatgtcga aagttcaaag ttcttaactt acaccatgct
     1681 tgctgaaaga tttggaatta gcaaaagcta cttatcttta attttaaacg gtaaaaagac
     1741 ttctgcagaa gcaaacagaa ttatagattc gattatcact atgtacgaat tgtaaaggga
     1801 ggaacagcta atgcaatatc tagaagcaaa aatccca
//
LOCUS       DAYLZO010000249         1831 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.264, whole genome shotgun sequence.
ACCESSION   DAYLZO010000249 DAYLZO010000000
VERSION     DAYLZO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.264"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            186..>1831
                     /locus_tag="ACGVJG_003131"
     CDS             186..>1831
                     /locus_tag="ACGVJG_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFJ6559011.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIR"
BASE COUNT          472 a          323 c          364 g          672 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc g
//
LOCUS       DAYLZO010000250         1793 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.265, whole genome shotgun sequence.
ACCESSION   DAYLZO010000250 DAYLZO010000000
VERSION     DAYLZO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.265"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..347
                     /locus_tag="ACGVJG_003132"
     CDS             <1..347
                     /locus_tag="ACGVJG_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559012.1"
                     /translation="FASPVLIPLALGFILVIGGLLVALLALIFSIYVLCISILGVGLF
                     MIISGISVFGQSILSGLFFIGVGLFATGSAVFISPLLLKGTKGLFYVMMNFVKWMGRR
                     FITKRDLHSAGV"
     gene            402..1193
                     /locus_tag="ACGVJG_003133"
     CDS             402..1193
                     /locus_tag="ACGVJG_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFJ6559013.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            1331..>1793
                     /locus_tag="ACGVJG_003134"
     CDS             1331..>1793
                     /locus_tag="ACGVJG_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFJ6559014.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGV"
BASE COUNT          628 a          287 c          392 g          486 t
ORIGIN      
        1 tatttgcttc tcctgtcctc attccactag cactggggtt tatattggtt attgggggac
       61 tattggttgc tttactagcg ctcattttta gtatctatgt tttatgtatc agtatcttag
      121 gtgttggcct attcatgatc atctcaggaa tcagtgtgtt tggacaatcg attctgagtg
      181 gattattctt tatcggtgtc ggtctgttcg ccacaggaag cgcagtattt atttcgccac
      241 tgttgctgaa aggaacaaaa gggctttttt atgtcatgat gaactttgtt aaatggatgg
      301 gacgacgttt tattacaaaa agagatctgc attcagcggg cgtttgaagg aggacacata
      361 aattgagatt gaaacatttt ttgattaccg gtattggtct gatgcttact ggtggcctcc
      421 tagcaggtgc aacttataca ttaggcgctc aaaagagtct gacttgggat aatggtcctc
      481 ggttgatcga tatggttact gagacaaaaa aaatcaagga tgcaaacaaa atcgtcatcc
      541 atgcagaaaa tcaaacagtt tcggtcatac gcggattgga ttttgagata aaaacgactt
      601 atgataagac agaaaagcca gaaataaagg agaaaaatgg cgtgttgact gtgaatgcag
      661 tcaaaaaatc aacaggtgca atcattagtt tagaagacac aagtgcacaa atcactattg
      721 ttgtccctgc agatcatgaa ttatctgaat tgacaatcga agggaataat agtgcaattg
      781 atatcaacga agtatcaatc aaaaaagtga atactaagac acatgatggc tgggtatcgt
      841 tcaatgaatt tacaggagga acaataactg cgaaagatga attaggctca cttcgattag
      901 atgaggccaa tgtggataac ttgatcgtca caggtgaaaa cagcaacttc catctagaag
      961 acagtttgat cagcaaagaa ggaaaagtca tcctaaacgg tggcaattta tctttgcatg
     1021 agatggaaaa cagtggctat actatccaaa cgtctggtga atcttatatt gaaaaagact
     1081 accagccaat aacaaatact ttgttagaac aaggacagaa aaagctaaag atcactgcta
     1141 aagacacatc gatcaatatt acgacggatc aagaggacaa tgaaacagat tgatcctgta
     1201 ctcaactgga tattttaaga aaaaacagca aagatccttc caatctttgc tgtttttcat
     1261 cgcttaacgc acataactat agtaaaataa gaaagaaaat atctaaaaag aaaacagggt
     1321 gaaagagtat atgaaggtag aagaaggtca aagagaaaag ttaaaaacag aaatggaaag
     1381 attacatact tatatcactc aactaagcca aaccttctat gatccagaca aagaaaaagt
     1441 gatggttaat tacccgaaca attcagaagg tcgccaatta gagcaagtgt accatgaagt
     1501 tttcaaacat ttattgacgg tgaagaaaga gctggattat tattctttac cgattatcga
     1561 tacaggaata ttgaagtatg atgggaagaa ggaacgattt atctttaaat ctgttcggga
     1621 agatttgcct ctttcagcag ggatggatct agaggtcttg gtagaagact attttacaga
     1681 agaaaagcac tgggttcgaa caaaattgga ttatttaccg caggcagccg gagggacaca
     1741 ggcaagcggt tggtacatca ctgaagacaa agagttagaa ctagaaggtg taa
//
LOCUS       DAYLZO010000251         1764 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.266, whole genome shotgun sequence.
ACCESSION   DAYLZO010000251 DAYLZO010000000
VERSION     DAYLZO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.266"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1235
                     /gene="lysS"
                     /locus_tag="ACGVJG_003135"
     CDS             <1..1235
                     /gene="lysS"
                     /locus_tag="ACGVJG_003135"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFJ6559015.1"
                     /translation="REGQIQIYVRKDQVGDEAYEVFKHADLGDFFGVTGQIMKTDTGE
                     VSIKASEITILSKALRPLPDKYHGLTNIEQRYRQRYLDLISNRESFDRFMKRSQIISE
                     IRRYLDGNGYIEVETPVLHNEAGGAAARPFITHHNALDIDLYLRIALELHLKRLIVGG
                     MEKVYEIGRVFRNEGIDTTHNPEFTMLEAYTAYTDFNDVMNLTEGIIRNAAEKVLGTA
                     KITYDGQAVDLESDFKRIHMVDAIKEQTGVDFWQEMTLEEALALAEKHNVEVTEAMGV
                     GHVINEFFETFVEETLTQPTFVYGHPVEVSPLAKKNPEDPRFTDRFELFIVGREFANA
                     FTELNDPIDQRERFEAQEKERELGNDEAHGVDEDFLEALEYGMPPTGGLGIGIDRLVM
                     LLTDAQSIRDVLLFPTMR"
BASE COUNT          667 a          301 c          351 g          445 t
ORIGIN      
        1 atcgtgaagg ccaaattcaa atttatgtaa gaaaagacca agtaggtgat gaggcctatg
       61 aagtattcaa acatgcagac cttggagatt tctttggtgt aactggacaa atcatgaaaa
      121 cagacacagg cgaagtttct atcaaagctt cagaaatcac gattttgtca aaagcacttc
      181 gtccactgcc agataaatac catggcttaa cgaatattga acaacgctac agacaacgct
      241 acttggattt aatcagtaat cgcgaaagct ttgaccgatt tatgaaacga agccaaatta
      301 tcagcgaaat ccgtcgctat ttagatggca atggctatat tgaagtagaa acaccagtat
      361 tgcacaatga ggcaggtgga gctgccgcac gcccatttat cacgcatcat aatgcactgg
      421 acattgattt atatttgcgt attgcgctag aacttcattt aaaacgacta attgttggcg
      481 gaatggaaaa agtttatgaa atcggccgcg ttttccgtaa tgaaggaatt gatactacac
      541 ataatccaga atttacgatg ttagaagcat acacagcata tactgatttc aatgatgtaa
      601 tgaatctgac agaaggtatt atccgtaacg cagctgaaaa agtactagga acagcaaaaa
      661 tcacatatga tggacaagct gtcgacttag aaagtgactt taaacgcatc catatggtag
      721 atgcaatcaa agaacaaact ggtgtagact tctggcaaga aatgacattg gaagaagcat
      781 tagctttagc cgaaaaacac aatgtagaag taaccgaagc tatgggtgtc ggacatgtta
      841 tcaatgaatt ctttgaaaca tttgttgaag aaacattgac acaaccgacg tttgtatacg
      901 gacatccagt cgaagtttcg cctttagcga agaaaaatcc agaagatcct cgattcacag
      961 atcgattcga actattcata gtggggcgcg aatttgctaa tgcctttaca gaactgaacg
     1021 atccaattga tcaaagagaa agattcgaag cgcaagaaaa agaacgtgaa ctaggaaatg
     1081 acgaagctca cggtgtagac gaagacttct tggaagcctt agaatacggt atgccgccaa
     1141 ctggtggtct aggcatcggg atcgacagat tagtaatgtt gctaactgat gcacaatcaa
     1201 ttcgggatgt tttgctattc ccaacaatga gataatcgaa taaaagctgt ctaaggaatt
     1261 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
     1321 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
     1381 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
     1441 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
     1501 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
     1561 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
     1621 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
     1681 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
     1741 ccaaaaatga atcgggaaac actc
//
LOCUS       DAYLZO010000252         1760 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.267, whole genome shotgun sequence.
ACCESSION   DAYLZO010000252 DAYLZO010000000
VERSION     DAYLZO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.267"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..734
                     /locus_tag="ACGVJG_003136"
     CDS             <1..734
                     /locus_tag="ACGVJG_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFJ6559016.1"
                     /translation="DWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLTL
                     LPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNVT
                     PYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYISE
                     DLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRDM
                     YKKYTGKTGCLDSTYAADIEERREQ"
     gene            731..1273
                     /gene="sat4"
                     /locus_tag="ACGVJG_003137"
     CDS             731..1273
                     /gene="sat4"
                     /locus_tag="ACGVJG_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFJ6559017.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1366..>1760
                     /locus_tag="ACGVJG_003138"
     CDS             1366..>1760
                     /locus_tag="ACGVJG_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFJ6559018.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCP"
BASE COUNT          606 a          273 c          412 g          469 t
ORIGIN      
        1 atgactggct taatcaattt gggaatataa taatgatgca aaagccggag gatatggaat
       61 tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat tacaataaaa
      121 ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc gataaattaa
      181 taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg actgatatag
      241 attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat gaattttgga
      301 atgtaacacc ttatgttatt aaaggattgt gccgtaagga aattttattt gctattgatc
      361 attttaatca gattgttcgc catgagctgc tgagaatgat atcatggaag gtcggcatcg
      421 aaacaggctt taaattaagt gtaggcaaga actataagtt tattgaaagg tatatatccg
      481 aggatttgtg ggagaaactt ttgtccacct accggatgga ttcctatgaa aacatatggg
      541 aagcattatt tctatgccat caattgttca gggcggtatc cggtgaggtg gcggaaaggc
      601 ttcattatgc ctatccggag tatgatagga atataacaaa atataccagg gacatgtata
      661 aaaaatacac tggtaaaacc ggctgcctgg atagcacata tgccgctgat atagaagaga
      721 ggcgggaaca gtgattacag aaatgaaagc agggcacctg aaagatatcg ataaacccag
      781 cgaaccattt gaggtgatag gtaagattat accgaggtat gaaaacgaga attggacctt
      841 tacagaatta ctctatgaag cgccatattt aaaaagctac caagacgaag aggatgaaga
      901 ggatgaggag gcagattgcc ttgaatatat tgacaatact gataagataa tatatcttta
      961 ctaccaagac gataaatgcg tcggaaaagt taaactgcga aaaaattgga accggtacgc
     1021 ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag gcagcgcgct
     1081 tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa tgcttgaaac
     1141 ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca aaatcggctc
     1201 cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta
     1261 tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc
     1321 ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga
     1381 atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga
     1441 atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg
     1501 acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta
     1561 tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg
     1621 agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa
     1681 caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc
     1741 gacatatcgg attgtcccta
//
LOCUS       DAYLZO010000253         1713 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.268, whole genome shotgun sequence.
ACCESSION   DAYLZO010000253 DAYLZO010000000
VERSION     DAYLZO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.268"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(157..612)
                     /gene="tnpA"
                     /locus_tag="ACGVJG_003139"
     CDS             complement(157..612)
                     /gene="tnpA"
                     /locus_tag="ACGVJG_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFJ6559019.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            855..1713
                     /locus_tag="ACGVJG_003140"
                     /pseudo
     CDS             855..1713
                     /locus_tag="ACGVJG_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT          552 a          336 c          325 g          500 t
ORIGIN      
        1 ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag gacgggtgta
       61 tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga tttttaaaag
      121 accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg gttctacgta
      181 ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac ggatgtattt
      241 ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg accaaaagga
      301 tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta ccgcactacg
      361 acctttcaga taccccataa aactggatat acatattttt ggtggtactc gaacaagcat
      421 gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca tttcgcacaa
      481 cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt ttcgttgata
      541 ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta aacttttatc
      601 gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc actcactagt
      661 atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa cgcgcggttt
      721 tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa taaaagggtc
      781 taaaactttt aagttttgta ccgaccccca aaagttagac caaaaaatct aacttttgga
      841 ggtcggtatt ttttatggct aagtatactt ttgaacttaa attaaagatc gttcatgatt
      901 acttggatgg taaaggcgga agtgattatc tggcaaagaa atattccatt aaggctccat
      961 cacaagtgaa gcgctggatc aatgcctatc aagaatttgg tgaagagggt ctagtaagaa
     1021 agcgacaaaa gaaaaaatat tctgttcaat ttaagctgga tgcgatagag ttatatctaa
     1081 caagtgagct gtcctatcgt gaagttgcca acacacttaa catgaacaac cctaatttga
     1141 tcgctagttg gatgcgtcaa ttccgtgagg gtgggatcga tggactctca aaaacgaaag
     1201 gatgtccacc tatcttgtct aagaaaaatg aacccaaaaa taagaaaaaa tctacaacta
     1261 aagcaacgtc taaagaacgt gaacgcatcg aggagttgga gaaacgagtc cgttcccttc
     1321 aaattgaaaa tgccttttta aaagaattga ggaagctgcg aaaacaggaa gcccaacaac
     1381 gacgaatgaa gcaatcgcac gaatcatcgc aagcctccga ggaccgttta aattagttga
     1441 gcttctagaa actttaaagt tccctaaggc aatctttatg tactggcaaa aacgattgga
     1501 ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaagttcggg agaagcacaa
     1561 ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa
     1621 aaagaaagtt cagcgcctga tcaagaagct aggaataaaa gtaacgacat acacacgtaa
     1681 atcacggcgc tacaattctt atcgaggaca agt
//
LOCUS       DAYLZO010000254         1706 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.269, whole genome shotgun sequence.
ACCESSION   DAYLZO010000254 DAYLZO010000000
VERSION     DAYLZO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.269"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..155)
                     /locus_tag="ACGVJG_003141"
     CDS             complement(<1..155)
                     /locus_tag="ACGVJG_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFJ6559020.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKIS"
     gene            330..854
                     /locus_tag="ACGVJG_003142"
     CDS             330..854
                     /locus_tag="ACGVJG_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFJ6559021.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            870..>1706
                     /locus_tag="ACGVJG_003143"
     CDS             870..>1706
                     /locus_tag="ACGVJG_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFJ6559022.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESA"
BASE COUNT          586 a          287 c          375 g          458 t
ORIGIN      
        1 gagattttat ctgacacact agatcggttc atcgaaagaa taaaattcct gatcaatatg
       61 gcagaaacaa tcaaagaaac gaatgtggag aaacaaacga caaggatgat cgtgttctgg
      121 agctttaatg gttttttctt aaaaaaagga aacataggct acctcgaaag aatttattgt
      181 gaaaatttta acatgaaaac aaaaatttaa caaattgtca acgataaaca caaccttcct
      241 ataaaaaaca ccactgagtc ggtaaaagaa gctatacaga attttcaaac attggtatga
      301 tagacgtaga aatggaagga ggcgtgtata tgataggggg aatcgcactt gagacgattt
      361 attggtatac attgctgatt tgtgcaggat tggcagtcct attgattatt tttggagatg
      421 tattcgattt tgatggcccg cttgatccga tgcttattat cccttggtta actttcacaa
      481 gcttatttgg ttttctaggt gagcgtttaa cagatcaatc atctttattt ttatttattg
      541 ctagtggagg catatcgata gtgttggtat ttttgcttaa tttctatata ttgatgccga
      601 tgaaacatgc cgaatcaacg atgtcgtctt ctgaaaaaac tctggaagga caagtagctg
      661 ttgtgatcac accgatccct gtacgaggta tgggagaaat tcagttgaaa agtgttaccg
      721 gttccattag tcgtccagct tgtttttaca gcccccaaga acagacagct gagcggggaa
      781 cgaaagtatt gatcattgag atcaaggaaa gaatctgtta tgtcatgcct tatgaaagca
      841 tgataaaagc ttaaaggagc gtgtgttaaa tggaaatacc agttgttttt atcgcgatcg
      901 caatcgcagc tttgatttta ctgatgttgc tgatcgtctt tgtagcgaag taccagacag
      961 caaaaccgga tgaagcgttg atcatcagtg gaagttattt aggaagcaaa aatgtgcaca
     1021 ttgatgaaag caataacaaa atcaaaattg tgcgtggagg tggcgcattc gtcttgccag
     1081 tttttcaacg ttcgaatcgg atcagtctat tatcgagtaa attggacgtc tccacccctg
     1141 aagtgtatac ggaacaaggc gtaccggtga tgtgtgatgg tacttcgatc atcaaaatcg
     1201 gttcatctgt agaagagatt gctacagcag cagaacagtt tttaggcaag acaagagaag
     1261 aactggaaaa tgaagcaaga gaagtattag aaggacactt gcgctcgatt cttggttcta
     1321 tgactgtaga agaaatctat caaaatcgcg ataagttcag tcaaagcgta caagaagtcg
     1381 cttctgtaga ccttgcaaaa atggggttga tcatcgtttc tttcactatc aaagaggtca
     1441 gagacaaaaa cggttatctt gactctttag ggaaaccaag aatcgcccaa gtcaaaagag
     1501 atgcagatat agcagaagcg gaagctctaa aagaaacgcg tatcaaaaaa gctcaagcag
     1561 aaaaagaatc acaagcagct gaattacagc gacaaacaga gatcgcagaa tcattaaaag
     1621 aaaaagaact gaaattagca acctacaaac aagaacaaga tgttgctaaa gcaaaagcag
     1681 accaagccta caatttagaa agtgcc
//
LOCUS       DAYLZO010000255         1678 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.270, whole genome shotgun sequence.
ACCESSION   DAYLZO010000255 DAYLZO010000000
VERSION     DAYLZO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.270"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..204
                     /locus_tag="ACGVJG_003144"
     CDS             <1..204
                     /locus_tag="ACGVJG_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFJ6559023.1"
                     /translation="HLPLLVIHGERDHFVPTEAAYTIQNATAGDKALLLVPEAEHLEA
                     SMKDPKTYWTVIFSFIEQRITMN"
     gene            301..594
                     /locus_tag="ACGVJG_003145"
     CDS             301..594
                     /locus_tag="ACGVJG_003145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6559024.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            712..948
                     /gene="secG"
                     /locus_tag="ACGVJG_003146"
     CDS             712..948
                     /gene="secG"
                     /locus_tag="ACGVJG_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFJ6559025.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1051..>1678
                     /locus_tag="ACGVJG_003147"
     CDS             1051..>1678
                     /locus_tag="ACGVJG_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFJ6559026.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKL"
BASE COUNT          559 a          283 c          380 g          456 t
ORIGIN      
        1 catctgcctt tattagtcat ccatggtgaa agagatcatt ttgtccctac agaagcagcg
       61 tacacgattc aaaatgctac ggcaggggat aaagcactgc tgcttgttcc ggaagcagaa
      121 cacttggaag catcgatgaa agatccgaaa acctattgga cagtgatttt ttcatttatt
      181 gaacaaagaa taacgatgaa ttaattttct cttctttttt gtaagtaatt cgaggataaa
      241 taattgttta tagatcagga gactgctaaa ataaggtagg aataaaaaaa tatcagttat
      301 ttgaggagtg cggcatatgg aaatcaaaga agaaaaaatc gtcttgcttt attcaatgat
      361 gaacaacaag agatcggaga aatgacatgg tcagatgcgg gcccagacat catgatcgtt
      421 gaccatacat ttgtagatcc agcatatcgt ggtcaaaagt tagctgaaaa gctagtgtat
      481 actggagtag aattagcaag acgagacggg aaaaagatca ttcctttatg cccttatgca
      541 aaaaaagagt ttgaaaagaa accagaatat caagacgtct tacgtcagtc ataagaataa
      601 cagctgaaag ttttgaactt tcagctgtta ttccatttat atagagttgt cacggacttt
      661 tatctatgat aaagttaaag agacgatatc ttttgggaag gaggctgcga tatgtataat
      721 ttaattttgg gaatcgtgat cgttttatcg attgttatgg tcattgcgat tatgatgcag
      781 ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg gtgcggatca actattcggc
      841 aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat caactgcagt tatgggagct
      901 atctggatga tcttattatt tgtcctagca tatttatctt cgaagtagtt gaataagcct
      961 ctcgcaaaac ggcgggcggc tttttttatt gcaaagagaa cgcttaagaa gaagttatga
     1021 aagaaactat ggtaaaatga ggcgaaagag gtgagacgag tgagacaatt accaaaaccc
     1081 ctttatgcaa aacatggaaa gcgagcggtt ttgctgcttc acgcttactc aggaagtcca
     1141 aacgatgttc gaatgctcgc acgatttttg gaaaaagcag attatacagt ttatgcgccg
     1201 ctttttaaag gacacggaac aatggagcca tatgatatct tgcaagaaaa agcagaaagc
     1261 tggtgggcag acactaaaaa agccatccat tttttacagt ctgagcagtt ttctgatatt
     1321 gccgtattag gattatcaat gggcggcatc tttgctgtac gagcactaga agaagaatca
     1381 gtgattggcg gcggtttttt ctgttcgcct ttgtctcctg taaaaacaaa cgtgcctgaa
     1441 aactttgaaa aatacgtacg tcaagtgctg aagacagcag gtaaatcaga gaaagaaatc
     1501 aatgaaaaag cagttgctta ccgctcgtta gctgaacaac aattaatgga tatacaagat
     1561 caggcagcaa tcgtagaaag taggttgtca gatatccaac agccgatatt tttagctcaa
     1621 gctgggaaag atgaaatgat cgaccctaat ggcgtatttg aaactgcacg caagttga
//
LOCUS       DAYLZO010000256         1667 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.271, whole genome shotgun sequence.
ACCESSION   DAYLZO010000256 DAYLZO010000000
VERSION     DAYLZO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.271"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            152..448
                     /gene="yidD"
                     /locus_tag="ACGVJG_003148"
     CDS             152..448
                     /gene="yidD"
                     /locus_tag="ACGVJG_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFJ6559027.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            632..1099
                     /locus_tag="ACGVJG_003149"
     CDS             632..1099
                     /locus_tag="ACGVJG_003149"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFJ6559028.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            1184..>1667
                     /locus_tag="ACGVJG_003150"
     CDS             1184..>1667
                     /locus_tag="ACGVJG_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFJ6559029.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     G"
BASE COUNT          583 a          251 c          371 g          462 t
ORIGIN      
        1 gagacttttt tcattgataa ataagaatca cagtggttct agtcgatatg aagtgagcca
       61 tgaagcagaa catgacaaaa gaaaacagaa gggaacgttg acgttagtcg cgatcccttt
      121 ttttagtaaa agaaaagaag gaatctaatt gatgaagaag atattgattt atttagttag
      181 aggatatcag cgctttattt cgcctgcatt tccacctagc tgccgttatt acccaacctg
      241 ttccaattat atgataaaag caattcaaac ccatggtgca ctaaaaggga cggtaatggg
      301 gactgcacgg atcttacgct gtcatccttt tgtcaaaggc ggtatcgatt atgtaccatt
      361 gacattccaa ttaacgcgaa ataagaaaga taaaaaagaa ccagattata aaaaggttgt
      421 gaaaaatacg agtagcactg agcgataaag aaacaatttg gaaaatagct tcttatattt
      481 tgcaaaattg tgagttattg ccgaagtgtt ggattttgaa caccgtcaaa aaaaggttgt
      541 gaaaaatacg ggtagcactg agcgataaag aaaaagagga aaattattgt caaagtaaca
      601 actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca agaaagattt
      661 accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag tatatgtttc
      721 cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt
      781 ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta gccccaacgt atcaaggaaa
      841 ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac tcgcttccag
      901 tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac atctctatca
      961 aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc attactctga
     1021 gcccattttt gaaaatggag atcaattaag ggatcaagta attttagcaa ttcacttacc
     1081 taaaaatagc tttaactaat aaactttcaa ttcgagaaaa tgctttcaaa tgctaaatag
     1141 atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa tattagtttt
     1201 tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa
     1261 gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat ttcaagttta
     1321 taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc ctggagtagt
     1381 gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc atggatttaa
     1441 tattttccga gatttggaaa ctctgtttca actcccagta acgattgaaa atgacgccaa
     1501 ctgtgccgga atggctgaat tttatgaagg tgctgcccgt gattatcaag atgttgcttt
     1561 tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa tcaataaagg
     1621 ggcacatctt tatggtggag aatttgggtt gatgttctta aatggcg
//
LOCUS       DAYLZO010000257         1662 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.272, whole genome shotgun sequence.
ACCESSION   DAYLZO010000257 DAYLZO010000000
VERSION     DAYLZO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.272"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            55..1217
                     /locus_tag="ACGVJG_003151"
     CDS             join(55..331,331..1217)
                     /locus_tag="ACGVJG_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFJ6559030.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
     gene            complement(1582..1655)
                     /locus_tag="ACGVJG_003152"
     tRNA            complement(1582..1655)
                     /locus_tag="ACGVJG_003152"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1619..1621),aa:Asn,seq:gtt)
BASE COUNT          593 a          297 c          328 g          444 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1021 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1081 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acaccggttg
     1141 gttaccgtaa ccagagattg gcgcagcgaa tccttgataa tgagctcgga tgtgctaacg
     1201 ctagcgaggc agtctaactt taactgttag ctcctgccga agatcgtcac atccgaggag
     1261 gctcattgtc aaggacaatc ggaatagcat acggaaagaa gttctgcacc ttataaaatt
     1321 tgtcaaaaaa actgttgcca ttccaatgag aaaaagcatg gaatgcgctg catcaggtat
     1381 cgaggcttag aaaaaaatca ggtgcatacg atgcttgttt ttgccgaaat gagtctgaaa
     1441 aaattggtca cttagcagtg ggagcatatg tcttcatatt ttttattata caaaaataga
     1501 caaaagtatc agtctacact cttagtaaag aggaaactga tacttttgtc tacagtatga
     1561 tatttcttac atctactttt actccggcag taggactcga acctacgaca tcatgattaa
     1621 cagtcatgcg ctactaccaa ctgagctatg ccggaataac at
//
LOCUS       DAYLZO010000258         1657 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.273, whole genome shotgun sequence.
ACCESSION   DAYLZO010000258 DAYLZO010000000
VERSION     DAYLZO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.273"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..502
                     /locus_tag="ACGVJG_003153"
     CDS             <1..502
                     /locus_tag="ACGVJG_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFJ6559031.1"
                     /translation="QNKGLDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAY
                     VEKLQKDGKKVGMAGDGINDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQ
                     TIELSRLTLRKIKQNLFWAFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCL
                     NRHMKK"
     gene            522..>1657
                     /gene="copB"
                     /locus_tag="ACGVJG_003154"
     CDS             522..>1657
                     /gene="copB"
                     /locus_tag="ACGVJG_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFJ6559032.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLA"
BASE COUNT          544 a          286 c          387 g          440 t
ORIGIN      
        1 ccagaataaa ggattagatg tttttatgct gacaggagac aacaaactgg cagccgaaac
       61 gattggtaaa caagttggta ttgatccaaa gcatatcttt gctgaagtac ttccagagga
      121 taaagcagcc tacgtagaaa aacttcagaa agatggcaaa aaagtcggta tggcaggcga
      181 tggaatcaat gatgcaccgg ccttagcatt agctgatgtc ggtatggcaa tgggaagtgg
      241 aacggacatt gcgatggaaa cagccgatgt gacgctgatg aatagcagtt tggcatccat
      301 tgcacaaaca atcgagttgt ctaggttgac attgagaaaa atcaaacaaa atcttttctg
      361 ggcttttgtc tataatacga ttggtattcc ttttgccgca ctaggctttt tgaatccaat
      421 cattgcagga ggagcgatgg cgtttagttc cgtcagtgta ttgttgaact ccctgtgctt
      481 aaatcgacac atgaaaaagt aagagaaggg atggagtaaa aatgaatggg aacgatatgg
      541 agaaaaaaca tgagcataaa aaagaagtag taaacaaaga aagtacagaa tctcatgaaa
      601 aacagatgga gatgaaacat gaccatagtc agatggatca ttccatgcac atgggacata
      661 accatggaga tatagcaaac agcaaacaaa tggaccattc gatgcacatg gatcacgaac
      721 acggcggcat ggatcattcc atgcacatgg ggaatttcaa acaaaaattt tggctatcgc
      781 ttatcctagc tatcccaatc attctttttt caccaatgat gggaatggaa tttccatttc
      841 aagtaacgtt tcctggttct gattggctcg tattgatctt agcaactata ctatttatct
      901 atggcggtca gccattttta agtggagcta agatggaatt gaaacaaaaa agtcctgcta
      961 tgatgacact gatcgcgatg gggattacag ttgcatatat ttatagtgtc tattcgttta
     1021 tcgcaaatct gctcaatcca catacacacg tcatggattt cttctgggaa ttggctactt
     1081 tgatcgttat tatgctacta ggtcactgga ttgaaatgaa tgctgtgtcg aatgccagca
     1141 atgctttaca aaaactagca gaattactgc cagaatcagt gaaacgcttg acgaaagatg
     1201 gaaaagaaga gacagtttca ttgaaagaag taaacgaagg tgatcgtttg atcgtccggt
     1261 ctggtgataa aatgccaaca gatggtgtaa tcttgaaagg tgaaacaatt gttgacgaat
     1321 cagctgtgac tggcgaatca agaggaatca aaaaacaagc caatgacaaa gtgattggcg
     1381 gttctatcaa tggggatgga acgatcgaaa tcgaagtaac tggaactggt gaaaatggtt
     1441 atctagcgaa ggttatggaa atggtgcgaa aagcacaagg agaaaaatcc aagctagagt
     1501 cattatctga caaagtggca aaatggctgt tttatgtcgc attaattgtg ggtatcctgg
     1561 cattcatcgc ctggttgttc ttgacagatc ttcctaatgc attagaacgt atggtaacag
     1621 tctttatcat cgcctgtcct catgctctag gtttagc
//
LOCUS       DAYLZO010000259         1621 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.274, whole genome shotgun sequence.
ACCESSION   DAYLZO010000259 DAYLZO010000000
VERSION     DAYLZO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.274"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..462
                     /locus_tag="ACGVJG_003155"
     CDS             <1..462
                     /locus_tag="ACGVJG_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFJ6559033.1"
                     /translation="ALNTGYHEPADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISI
                     GKNVFINRQVMFVDLGGICLEDSVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNA
                     WIGAGATILPGVTIGENAIVAANATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            529..1266
                     /locus_tag="ACGVJG_003156"
     CDS             529..1266
                     /locus_tag="ACGVJG_003156"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFJ6559034.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            1497..>1621
                     /locus_tag="ACGVJG_003157"
     CDS             1497..>1621
                     /locus_tag="ACGVJG_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFJ6559035.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSET"
BASE COUNT          561 a          294 c          340 g          426 t
ORIGIN      
        1 gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga aaatatcatt
       61 agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa
      121 catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt
      181 ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat cacggtgaac
      241 catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca catcaagaaa
      301 aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg cgagaatgcc
      361 attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat cgttgcagga
      421 atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag atgaaataag
      481 agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat gaatgattta
      541 tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc aacaacgaag
      601 ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga tcgcctaaaa
      661 gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga tgtaagagat
      721 tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg aatcgatgta
      781 ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca ccgtgatgaa
      841 tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc cgccgtactt
      901 ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt ggcgggacat
      961 gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg
     1021 gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat ctctccagga
     1081 gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct tgaacttcat
     1141 gaagcgcaaa aagaatgggg attaacatct gaagatatag cttctgctgt agcatttgcg
     1201 atagaaacgc cagaccggat gtctgtcagc aacatgatta tccgtccgac gacacaggaa
     1261 gtataatcca taacggataa tatccagaga gaattacttg ttaaatagtt attcaaacga
     1321 ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt ttatgcagaa
     1381 aaagcaaatt tattaaaaag aaacaaatgt tatttaaata tggacattga cttgctttta
     1441 cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga aaaaagatga
     1501 aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt gccaacacaa
     1561 cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt tcttcagaaa
     1621 c
//
LOCUS       DAYLZO010000260         1615 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.275, whole genome shotgun sequence.
ACCESSION   DAYLZO010000260 DAYLZO010000000
VERSION     DAYLZO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.275"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..999
                     /locus_tag="ACGVJG_003158"
     CDS             <1..999
                     /locus_tag="ACGVJG_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HFJ6559036.1"
                     /translation="KRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKL
                     GEGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDD
                     TSVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSD
                     PVQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDS
                     IAVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQEN
                     TSTSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            1023..>1615
                     /locus_tag="ACGVJG_003159"
     CDS             1023..>1615
                     /locus_tag="ACGVJG_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFJ6559037.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFA"
BASE COUNT          525 a          313 c          387 g          390 t
ORIGIN      
        1 aagcgctact atatccatgg atatgaaaat gcagtgactg tcaaactttc cagtgcaaac
       61 cgagttcagt tgaatgcaga ggcaaacgaa gatacacgga tgttccgcgt cacagcagat
      121 atcactaagc tgggcgaagg aacacatgag gttccgctaa aaattcaaaa tttgagcagt
      181 gctgtcacag caaagatcga accatcgact attacagtaa cggttgagaa aaaggtaata
      241 aaagatttcg atgtggaaac gttgattcct tcaacgttca caccatctgg atatgaactt
      301 gatgatactt ccgttagtcc taaaaaagtc tctatcacta caggtgacaa aacattggca
      361 gaaatcaaac gagtagtagc tgatgtggac gcttcgatgg tcacagatga tgggattaat
      421 agtgaggtcc caatacaggc gctgaatgca gcaggggaag tattaagtat cgtctctgat
      481 ccagtgcaag taacagtgag agctgattcg atcaagccaa gcaagtcggt acgactttat
      541 gggatacagc aaggaacacc agctgccggg gtagaaagct atgatttcag tttttcaaaa
      601 ctggaagcag aagtaagcgg ctctagcgat ttgcttgcat cgatcggtga cagtattgct
      661 gtaccgatca atgtctcagg tatcaatcat cgaacaactc gtaaaattga aataccagta
      721 gaagaggggt taagtatcaa gcccgaatca gttagtgtgg agattacacc cgtgttgcaa
      781 acgtcaagca gtagctctgc ttcgacgagc agtcggacac aagaaaatac aagtacgagt
      841 agaagtattt catctgctga ttcaggaacg gaaccatcga caacaaccat ttcttccagt
      901 tcggaatctg ggacaagcag ctcgaccaca agtacagaag aaacatcaga aacaagcgaa
      961 tcaacacaaa tgagcagaac aaattcctca gaaaattaag ttaaaggagt aattttacaa
     1021 ctatgggtaa atattttgga acagacggcg tacgcggtga agccaacaag gaacttacgc
     1081 cagaacttgc atttaagctg ggacggtgcg gaggttatgt cctaagccag catgaagaag
     1141 aaggaaaacg cccacgcgtg ttagtagggc gggatacaag aatctcaggt caaatgttag
     1201 aagaagcgct gatttcagga cttttatcgg tgggtatcga agtgttccag ctaggtgtga
     1261 tctctacacc aggtgttgct tatctgacac gtttgcaaaa agcaagcgca ggggtcatga
     1321 tttctgcttc tcataatcca gcacaagata atggaatcaa atttttcggt gcagatggtt
     1381 ttaaactagt agatgaccaa gagctagaaa ttgaagcatt attagatgct gaagtggatg
     1441 aattgcctcg tccatcagct gaaggattgg gaactgtaga agaatttcca gaaggcttat
     1501 tgaaatattc tcaattcctt gtacaaacga tcaacggtga cctttccggt ttgaccattt
     1561 gtgtcgatgc agcaaacgga gcaacttcaa caagtgttaa tcgtttgttt gccga
//
LOCUS       DAYLZO010000261         1604 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.276, whole genome shotgun sequence.
ACCESSION   DAYLZO010000261 DAYLZO010000000
VERSION     DAYLZO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.276"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..455
                     /locus_tag="ACGVJG_003160"
     CDS             <1..455
                     /locus_tag="ACGVJG_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFJ6559038.1"
                     /translation="SSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRT
                     MLIVSPAMIVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNP
                     PKQVVTPRIDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(634..813)
                     /locus_tag="ACGVJG_003161"
                     /pseudo
     CDS             complement(634..813)
                     /locus_tag="ACGVJG_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          503 a          338 c          246 g          517 t
ORIGIN      
        1 aatcatcttt ctacggcatc gatttaggct caccaagtat ggtacttgtt gccattgcag
       61 gggtcgctta tctgcttcaa ggatacatct caacgatcgg tattcctgaa gaacagaaaa
      121 agacgatgcg tacgatgctg atcgtctctc ctgctatgat cgtcttcatg tcgatcagtg
      181 cgccagccgg tgttacactt tactgggtag tcggtggtgt gttcagctgt ctgcaaacat
      241 tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac
      301 aaaacccacc aaaacaagtc gtcactccaa gaattgatgt gacagaatca gctaaaccag
      361 ctgacagcaa acaagcaacc aaacaaatca agcaacccaa aaacagtaca aacaataaag
      421 gccgcaacgc aggaaagcag cagaagcgga agtagatagt aaataagagt gtaataaaaa
      481 ttcattaaca gttttagcaa ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt
      541 cgatattttt atttaagtga atgtttaatt tgaatcatcg attgatcgct atctaattgc
      601 agcagcatct ttttatgaag aaacaacttt ttcttatcta tcaacgtaga caggtgttgc
      661 tgtttcttct aatagtgtac ttaattcctt aatattttgt tttccttctt ctaacatcca
      721 actactcgct ttttcttcgc cttcggtaac aaaaggcgcc actcccttcg cccatgttgc
      781 tcgcccacat aagacaccat tctccataaa ggacaacgcg gagataccac ccatctaaat
      841 aacatccata aacctttcta gtagaagaat agtgatagta tccatccctt tttctactgg
      901 gaatacaatt acacgattca aagcttgata cacaacattt cccttcttat aaaccgctat
      961 tgtaaccgaa ttgttttgaa tcaccttttt gggatagaaa ctggctcctt tccgcaactt
     1021 cttaaatgtt ggtttctttt atactttctt tgtagcaact acaaatttct tcaaatctct
     1081 aaatgtttgc ataacaaccg cctttctgac ttttactcag gatggtattt aaaaatcagc
     1141 aaaataaggc ttctctccat cttttttatt tcctagctgc tttaaagaca ctcttgctgt
     1201 tttttaatct ctatcaaggg ggctctgaat ctattttgat gaacttatct tattaatttc
     1261 tcaaatgagc tgtcagacac aactgtattt tttcaatctc ataccggaca actatgccag
     1321 cttaaaaaaa tttcctctgg caaaacttct ttattgaaaa atgacaatga aaaaagacca
     1381 ttctttttgt tgaatgctca ttgtgtaaac atatttcctg cgactgctct tcgaaccatt
     1441 ctcagtgcaa tccctaattt atctattaaa tcttctttta gttcctcatc tttcatattc
     1501 aaaatttcct ttgtatgtta cacaagtttg ttctctaaat aacgcgaatt gtaaaattaa
     1561 gttagaaaaa taaaaaggca tttatggtac actcaaattg tatt
//
LOCUS       DAYLZO010000262         1599 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.277, whole genome shotgun sequence.
ACCESSION   DAYLZO010000262 DAYLZO010000000
VERSION     DAYLZO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.277"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(77..625)
                     /locus_tag="ACGVJG_003162"
     CDS             complement(77..625)
                     /locus_tag="ACGVJG_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFJ6559039.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(684..1121)
                     /gene="zapA"
                     /locus_tag="ACGVJG_003163"
     CDS             complement(684..1121)
                     /gene="zapA"
                     /locus_tag="ACGVJG_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFJ6559040.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            1323..>1599
                     /locus_tag="ACGVJG_003164"
     CDS             1323..>1599
                     /locus_tag="ACGVJG_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3378 domain-containing protein"
                     /protein_id="HFJ6559041.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVL"
BASE COUNT          502 a          322 c          267 g          508 t
ORIGIN      
        1 ccggacatac gctacgcagc cttcttaaat aatctactct gtacaaaaaa cgaaatattt
       61 tccagcacta ttttccttaa ttcaaaatat tcgttaccca aagctcatac atcttgttgg
      121 taagaaatgg cgttctctca acgataaagc gagcgaaact atgctcgccg aattgatttt
      181 ggaccacatc taatggaaca aaggcaagta actgcaaaat caaaaatata gtaacgtaag
      241 ccatgattag tgacaggatg cctcctgcaa tccagtctac ctgtctcaag atcggcacat
      301 acgtcaatcc atggacaaaa acccccacaa atcgggtaag cagccagcca attagcaaaa
      361 taatcagaaa agcagttcct gcataaaaag cttcatctag gtgaaaggca aatacctgat
      421 caaaaaatgc taactttgat gttggcgtga cagctgggta tggaatgtat aattccaaat
      481 gacttgctaa tggtttgtaa aaatgctggg cagcaataaa tgacaataca tatccaatcg
      541 cgaatatgat ttgcaaagca aacccgcgac gtgctccaga aaaaaacgca attagcaata
      601 aaaataaaat cagcaaactc agcatgctac gttcctttct cttctaactt tttttaaatt
      661 tttcttattt tgataaaagg atcttaatta gaagattttt gtttaatttg tccttttcgt
      721 ttctcgttta aaatctgttg tgcttccaca tgatttttaa tggtatgatc tgcttgacca
      781 ttttctttca atacttcccg agcttcgttc tcgattgctt caatgcgttt cacacgattt
      841 tcaagctctg taaatttgat catttttttc tttaattctg ctgtttcttc ttccagctcc
      901 aagattcttt cttgtttctt caattgatcc gaaagcgcat tgacagccat caagattgct
      961 gcttgttcat tatccatttg tggtgataat tgtttcaatt ctgctaactg ttcattgatc
     1021 agcttcgtta cgatatccat atgatgcttg gtttcccgtc cgataatagt atatgtttgg
     1081 ttggcaatca ccgctttgta gcgtgttttt tcgtgcgcca tacgaatcat ttcctcctat
     1141 ttgatgaaag ttttatttag tgtaccatga agttggttct ttcaccgttc tccacaaata
     1201 tttttcacat acttaactat tatatgataa actttaaaaa aatgctatgt ctcgcaatct
     1261 cttagaaaac gaaaagtcgt gagttgcggt atagttttat gtataaaaac gaggagaatt
     1321 ttatgagcaa cgtcattatc aaagcaacaa acgaccaact tagtcaaatg aaagcatact
     1381 atcgctctta tttgttaaat aaacagattc cttatactag ctttgctgca aaaaaagacg
     1441 ggacgaccat taccgcttat acatccggaa aagtgatgtt tcaaggtacg aatgcagcaa
     1501 tagaagctga taaatggggg gaggcacagg ataaacaagc caagccagca aaaacagcct
     1561 cttctttgcc aaaaaatatc agtcaattat ccgttttag
//
LOCUS       DAYLZO010000263         1586 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.278, whole genome shotgun sequence.
ACCESSION   DAYLZO010000263 DAYLZO010000000
VERSION     DAYLZO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.278"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..439)
                     /locus_tag="ACGVJG_003165"
     CDS             complement(<1..439)
                     /locus_tag="ACGVJG_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFJ6559042.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSI"
     gene            625..1560
                     /locus_tag="ACGVJG_003166"
     CDS             625..1560
                     /locus_tag="ACGVJG_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFJ6559043.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT          500 a          267 c          329 g          490 t
ORIGIN      
        1 ggattgagcg ctgatactgt tgttttcccc gtttttcctg atggccattg ggttttcttt
       61 tacctttcat cggtagtgtg tcaatatcaa atattccttt agaaaataaa cgataaagtg
      121 ttctcatacc gcatgaaaca gacctttctt ttcgcccgat aatgacgtca ggcgtccaac
      181 caagagtgac tttctcttta atgtaatcta cttcatgagc aggtaattga atgacttttc
      241 taccacattt tttcttattt tctttatact ggtgccaata atcaagagca gtcttgcctt
      301 gcttgaactt attgactaca ttataaatag tttgtatagc acgtcccatt cggttagcga
      361 tttcaaccgg cttattatgt tgaagataat atgattctat cattgtcagt tcgtctatgg
      421 taagatgttt gtaggtcatt tatggttaca ctcctttgtt ttctttcgtc ggaaatacaa
      481 tttgagtgta ccataaatgc ctttttattt ttctaactta attttacaat tcgcgggtta
      541 taaaaatgaa cattgcatag tagagattct ttgattatac taattcaagg ttatatgaaa
      601 aaagagaaat gagatggatg atttatgaaa ctttggatga aaatcctttt aggcgtcgct
      661 ttagctctga ttgttgcgtt agtgtttgca gggaattatt tttataatta tgctgttgtg
      721 ccttctgaga aagacttttt agacgaagat acacctggta caacacagac atccaaagaa
      781 acggatgcac ataaatggtt tagttcaaaa gaaaaccgaa gcgattggac catcacttca
      841 aaagacggtc tgaaattatc agcgatttat cttcctgctg aaacgaaaag tgaaaaaaca
      901 gctcttgtgg ctcatggcta tatgggaaac gctgaaacga tgaccaatta cgcaaagatg
      961 tttcatgata tgggttacaa tgtccttgtt cctgatgcaa gaggacatgg aaaaagcgaa
     1021 ggggattaca ttggatttgg atggccagag cgtaaagact atgtacagtg gataaacaaa
     1081 gtgctagaaa acaatggaaa atcgcaagaa atcgttctat acggtgtcag catgggagca
     1141 gcaacagtta tgatgactag cggagaaaaa ctaccagata atgtgaaagc aatcattgaa
     1201 gattgcggtt attcttccgt tcatgatgag cttgcttatc agctagacga tatgtttagt
     1261 cttccagcat ttccgcttat gcaagtaacg agtctagtga caaaagtccg tgctggctac
     1321 tttttcggtg aagcaaatgc agtggaacaa ttgaagaaaa accagcgccc aatgctcttt
     1381 atacatggag acgaagatac atttgtacca tttgaaatgt tagataaggt ctatcgtgca
     1441 acgaagggac caaaagaaaa gtatgttgta aaaggagccg agcatgcgga agcatacaag
     1501 acagatcctg aaaaatatca gcaagtagtc caacaatttt taagccaata tgttcattga
     1561 acgagtttaa aagtagggga tgaaaa
//
LOCUS       DAYLZO010000264         1578 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.279, whole genome shotgun sequence.
ACCESSION   DAYLZO010000264 DAYLZO010000000
VERSION     DAYLZO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.279"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..191
                     /locus_tag="ACGVJG_003167"
     CDS             <1..191
                     /locus_tag="ACGVJG_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFJ6559044.1"
                     /translation="DAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVL
                     GKPLTSLEAALKEILDQE"
     gene            complement(523..>1578)
                     /locus_tag="ACGVJG_003168"
     CDS             complement(523..>1578)
                     /locus_tag="ACGVJG_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFJ6559045.1"
                     /translation="PLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFVDYFLSLKNPIA
                     NQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTATTNSDNKYAAQA
                     SKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMKIIDGKNAQVIV
                     GTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIANGKLIPITEVS
                     DDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAGIEVLLHMGINT
                     VDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTNGDKVQSLEIEP
                     ARDVKANDKIGSVSNKA"
BASE COUNT          449 a          298 c          334 g          497 t
ORIGIN      
        1 gtgatgcagc gtttgtcgaa aatctaaaag aagccggatt ccctcaagaa gcagctgaca
       61 tgtttttagc gatccaacat gatattaaaa aggatcagtt agctatttcc tctgatgatt
      121 tagaaaaagt attgggaaaa ccattaactt ctcttgaagc agcattaaaa gaaatattgg
      181 atcaggaata atgtataaag aggttgtgaa aaagccgatt agttttgagt atgaagaaaa
      241 attttgtgaa aatggctttc cacatttttg cataaatttg acttcatacc gataaactgg
      301 tttttgaaca ccgtttatca gaggttgtaa aaaagctgtt ctgcatcaag taataagaag
      361 agtcaaataa aaaatagctc ttcatatgtt acgtttggct ggacttatta ccgcggagtt
      421 ggcaggagaa cgccgttttt attcggaaaa aggagctgag acaagatttt tgtctcagct
      481 cctgtttgtt tatcgataaa aaagtctggg gaagacgatg gctcaggctt tgttggaaac
      541 agagccgatt ttatcatttg cttttacatc acgtgcgggt tcgatttcta agctttggac
      601 tttgtcgcca tttgtgaata caacgaccat atccgtgttt ttgcctgcag attgaatagc
      661 ggcaagatca actaaagcaa tcaattgacc tcttgcgact ttttgacctt cttttacata
      721 taaagtgaat ggttcgcctt ttaagtcaac agtgttgatg cccatatgaa gcaagacttc
      781 gataccagca tctgtttgga ttccgacagc atgtttcgtt gggaaaatgt ttgtgatcgt
      841 accagcgatt ggagagaaga tttctccatt ttctggtaat acagcatagc cgtcacccat
      901 cattttttcc gcgaatacat catctgaaac ttcagtgatt ggaatcagtt tcccatttgc
      961 gatagcgtaa atatcttgtt cgatcgttcc accggcaaat gtttctgttt taaccacggt
     1021 gtttgtttgt gcaggtcgtg ttgcagcacc accatgtagt tttgccatct catctgcaac
     1081 gaattgtact tctgtaccta cgatgacttg cgcatttttt ccgtcgatga tcttcatacc
     1141 aggtacgcca gtggctttga ttttattttg atcgatcagg cctgtatctt tgacttgcaa
     1201 acgaaggcgt gtagtacagt tatcgatcga agttacattg tcggctccgc ctaatgcagc
     1261 gtagattttg cttgcttgtg ccgcatattt attgtctgaa ttagtcgttg cagtatcgtc
     1321 atctgaaaga tctgcttcct cgcggccagg agtcatcaga tggaattttt tgatagcaaa
     1381 gttaaagcca aagtagtaaa tcactgctgt tactaatcct tggacgatca acatcaatgg
     1441 ctgattcgca atcggatttt tcaaagatag gaagtaatcg acaaatccgg cactaaaggc
     1501 aaatcctgct gtccaatgga agaatgcact cactgctaaa gaaatcccag tgaaaattgc
     1561 atgcaagaca tataatgg
//
LOCUS       DAYLZO010000265         1572 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.280, whole genome shotgun sequence.
ACCESSION   DAYLZO010000265 DAYLZO010000000
VERSION     DAYLZO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.280"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            125..1426
                     /locus_tag="ACGVJG_003169"
     CDS             125..1426
                     /locus_tag="ACGVJG_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFJ6559046.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          509 a          322 c          280 g          461 t
ORIGIN      
        1 gctctataat ttctaggact gatagaaaat cattctatcg gtcccttttg agcatgaaaa
       61 aacaaaacat acagaagctc cgttagaatt gaatcaccac aacacaatca aaggagcgtt
      121 ctgtatgcct gatcttcatc atatcaaact actacttgga atcaaagata aaaacatttt
      181 catcacaaat gtggaatcga agagcattaa gaatatcaag agtcttgttg tttctgcgac
      241 gttaagtaaa gaaattcgtc gctgtccttt gtgtaaacag atgaatcatg aaggcatgat
      301 cgttaagaac ggaaagaaaa aatcgctcat tcaacttaat aaatgtgcga accaactgac
      361 ttatcttgct ttagcgaaac aacgctacca ctgtcgtggc tgccacacct attttacggc
      421 gaatacctac attgtcgata gaaactgctt tatcgctaaa caagtgcgct ataaaatttt
      481 agaagagcta actgaaaaac aagcaatgac gacaattgct aagcattgcg gcgtttcttg
      541 gtcgaccgtt tctcggacac tcgcttctct gatccccatg acaaaagtaa aaagaaattg
      601 gttaccgcgt tgtttattaa tggatgaatt tcgttcactg aaaaatcaag tcggtcccta
      661 ctccttttct tgtatggatg gcgatactgg aaaattactg gatattcttc cttcacggaa
      721 gaaaaaggct ctcgtttctt attttatgca atttgaacga cgcgcacgat tgaaggtaaa
      781 ggtcatcgtg acggacatga atgtttctta tgcctctctg ataaaggaat gttttccaaa
      841 ggcaaaactc gttgtggatc gttttcacat tgtcaaacat ctcattcgta aatttgagga
      901 tattcgcgta cgaatcatga aaagctttga tcgaaacgat ccaacacaag ccaaatatta
      961 tcgacaatta aaggcgttgt ctcggcttct gatcaaacga caggacaagc ttgtttatga
     1021 caaatggacc aaatggcgca attttggttg ggcgtatctc accgaaagtg aagtggtgga
     1081 tcgtctgctt tcgatctctg acgaattacg aatcgcctat ttttactatc aagaaattct
     1141 tcaagcgttt cacgacaaag aggcggacac tttctttaaa cttgtaagaa cgatgcccaa
     1201 ttccgttcca aaagaactgc attctataaa aaaagctttt ataaactatg aatccggcat
     1261 tcgtttagcc ctcgaattgc cttattcaaa tgccaaaata gaaaatctgc atacccacat
     1321 caaagcattg aaacgagtcg cttatggctt tcgaagcttt cggaaaatga agaccagaat
     1381 tttcttacta aataatctga ttacctatga atcaaaaaac atctaaagta gaaaaggaat
     1441 tctaccttag atgttcttat ctcactctat cagtcctatt tgacaaagag ccgctctttt
     1501 acaagccaca ccagcttttg atacgttgtt tattttacat attcatgaac aagttcaacg
     1561 acttcttcca tc
//
LOCUS       DAYLZO010000266         1561 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.281, whole genome shotgun sequence.
ACCESSION   DAYLZO010000266 DAYLZO010000000
VERSION     DAYLZO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.281"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..920
                     /locus_tag="ACGVJG_003170"
     CDS             <1..920
                     /locus_tag="ACGVJG_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFJ6559047.1"
                     /translation="RARKQMLVDYGFRLPSALDNRPLRLEEFEKHVNQIIYVSATPGP
                     YEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKDQRVFVTTLTKK
                     MAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGINLLREGLDVPEV
                     SLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMRLAMDETSRRRT
                     IQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTKEEKDTLLMKLE
                     KEMKNAAKALDFETAANVRDMILELKASK"
     gene            930..>1561
                     /locus_tag="ACGVJG_003171"
     CDS             930..>1561
                     /locus_tag="ACGVJG_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFJ6559048.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIV"
BASE COUNT          538 a          276 c          365 g          382 t
ORIGIN      
        1 accgagcgag aaaacagatg ctagtagatt acggtttccg tttgccatct gctttagaca
       61 atcggccact tcggttagaa gagtttgaaa aacatgtcaa tcagatcatt tatgtctctg
      121 cgacgccagg accatatgaa catgaacaaa cagataccgt tatccaacaa atcatccgtc
      181 cgacagggct gcttgaccca gtgatcgaag ttcgtccaat catgggacaa atcgatgatc
      241 tagtaggcga aataaatgaa cgagtcgaaa aagaccagcg ggtgtttgta acgacattga
      301 cgaaaaaaat ggcagaagac ctcacagatt atttcaaaga attagggatc aaagtcaaat
      361 atttgcatag tgatatcaaa acgctggaac gaacagaaat cattcgagat cttcgattag
      421 gtgaatttga tgtgttagtt ggaatcaatt tgcttcgtga aggattagac gtacctgaag
      481 tttcccttgt tgctatcttg gatgcggata aagaaggctt tttacgtagt gaacgttcct
      541 tagtccaaac gattggacga gctgctcgga atgaagaagg taaagtaatc atgtacgcag
      601 acaaagtaac agattctatg cgtctagcga tggatgaaac atcaagacgc cgtacgattc
      661 agcagaagta caatgaagaa catgggatcg ttccaaaaac gatcattaaa gaaatccgag
      721 atttgatttc tattacgaaa gaatcggaag atgatacaaa agaagcggta caagtctcgt
      781 atgaagaaat gacgaaagaa gaaaaagata cgttattgat gaaattagaa aaagaaatga
      841 aaaatgcggc gaaagcattg gattttgaaa cagccgctaa tgtgcgagat atgattttag
      901 aattaaaagc ttcaaagtag gaggacaaca tggctaacga taaaattacg attcacggtg
      961 cacgtgccca taacttgaaa aatatagatg taacgattcc acgagataaa ttcgtggtcg
     1021 ttacaggttt gtccggttct ggaaaaagtt ccctagcatt tgatacgctg tacgctgaag
     1081 ggcagcggag atacgtagag agcctttctg cttatgctcg tcagttttta ggacaaatgg
     1141 ataagccgga cgtagatagc atcgacggat tgagtccggc gatttctatt gatcagaaaa
     1201 caacaagtaa aaatcctcgg tcgacagtag gtactgtgac ggaaatcaat gactatttac
     1261 gcttgttgtt tgcacgtgta ggacatccga tttgtcccaa cgatcatatc gagattacca
     1321 gtcaatcagt agatcaaatg gcagatcaag taatggcttt gcctgagcgc acaaagatcc
     1381 aagtgttggc accgatcgtg gtaaagaaaa aaggacaaca taaaaaaata ttcgaacgaa
     1441 tccaaaaaga gggatacgtc cgcgttcgag tcgatggtga gatctatgat ttaagtgaag
     1501 tgcctgagct ggagaaaaac aaaaaacatg atatcgcgat cgtcatcgac cggatcattg
     1561 t
//
LOCUS       DAYLZO010000267         1510 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.282, whole genome shotgun sequence.
ACCESSION   DAYLZO010000267 DAYLZO010000000
VERSION     DAYLZO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.282"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>1510
                     /locus_tag="ACGVJG_003172"
     CDS             <1..>1510
                     /locus_tag="ACGVJG_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFJ6559049.1"
                     /translation="IILFATSIGLFFLFAVRFSYIVIGGHVAGTSLAEKTKQLYQGSE
                     VVKAKRGTIYDRNGVALAEDASSYSIKAILSKTYTSGDKKLYVEEKNFDKIAEILHKN
                     LSIDKKDALNILEDGAKKELYQVEFGSYGKNISQETKQNIEADMKKEGVAGLYFVDHQ
                     ARMYPNGVFSSHFIGYAVPDKDENGLVGKLGLESAYNDILSGKDGKIIYQKDNFQNPL
                     PGTVAEEEKAVDGQDIYTTLDSRLQSYLETLMDQVNEEYQPEELTAVLMKAKTGEILA
                     MGQRPTFNPETMEGLTGKDAIWRNFLVQDSYEPGSTMKVFTTAAAIEEGEFNENETFQ
                     SGKIQVEDATINDHDFGEKGVLTMRQALSWSSNVGMVILEQRLGGRWYNYLQKLGFGQ
                     STHSGLDDEVNGALPTLNIVDRAMSAYGQAVGVTNFQMMKAFTSIANNGTMIQPRYIS
                     KVVDPQTGEERTTQTEVLGQPFSKETTEKVREYMRDVVESENYGSAYGVYSVP"
BASE COUNT          526 a          254 c          335 g          395 t
ORIGIN      
        1 atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg tttctcgtat
       61 atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa acagttgtac
      121 caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa cggtgtggcg
      181 ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac atatacctct
      241 ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga aatcctgcat
      301 aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg cgcaaaaaaa
      361 gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga aaccaagcaa
      421 aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt cgaccatcag
      481 gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc agttccagac
      541 aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa tgatatcttg
      601 agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc tctgccaggt
      661 acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac tttagacagc
      721 agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata tcagccagaa
      781 gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat gggacaacga
      841 cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat ctggcgtaat
      901 ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac tactgcagct
      961 gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa aatccaagta
     1021 gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac catgcgtcaa
     1081 gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt aggcggacga
     1141 tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg attagatgat
     1201 gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag tgcttatgga
     1261 caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat tgcgaataac
     1321 ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac tggagaagag
     1381 cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac agaaaaagtc
     1441 cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgctta tggtgtatat
     1501 agtgtacctg
//
LOCUS       DAYLZO010000268         1502 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.284, whole genome shotgun sequence.
ACCESSION   DAYLZO010000268 DAYLZO010000000
VERSION     DAYLZO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.284"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(13..1083)
                     /locus_tag="ACGVJG_003173"
     CDS             complement(13..1083)
                     /locus_tag="ACGVJG_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFJ6559050.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(1254..>1502)
                     /locus_tag="ACGVJG_003174"
     CDS             complement(1254..>1502)
                     /locus_tag="ACGVJG_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFJ6559051.1"
                     /translation="AKLIYHPDTLEIIGGQLISQEFLLSDLNLLADIVKHKTTIPQLA
                     VEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRVDKRL"
BASE COUNT          460 a          326 c          227 g          489 t
ORIGIN      
        1 tcttcctcct tcttatccaa ttgccttcaa cggatcgatt tttacgattg tccgaacaga
       61 aaatagtgcg cctaacacag ctactactaa catcaacaag ctaatgacgc caaaaaacag
      121 ccaattgctt tggaatggaa ccgcatcagg tagaacaagg gatgttccca ctgttcctaa
      181 tagaccaaga agaataccaa caaatgataa aatgaatgtt tgtgccacta cagaacgcgc
      241 aataaatcct cctgtgatcc cttgtgcttt cattacacca aagatattaa tcttttgcat
      301 cgtcagtaca taaatgaaaa tcccgatcac aatagccgca ataacgatca aaaatccgat
      361 cataaatcca aaagtcaata cttgcgcatt atatccaggt aattcattga tgaatttaga
      421 aattttgatc tgttctaaat cttcaggtaa attactgatt ttacctctga caactattgc
      481 gttaatccgt gcattttcac ttgtatcttc tttttcgaag cggatttctt ggtaagcatt
      541 gatggttgta tagagaactg gtgagacatt gaatttggca tgatccgtaa agccagttag
      601 tttaaacgtc ttgtcactac cagataactt aactgtatct cctatggcta gaccatattc
      661 ttctttcaaa ctaatatcgc ctactgcctc atcatcgtta tcaaaagctt ttccttcaac
      721 aagattgggc atgatgaatt gatttttatc gataccgaag aagctaacat tgatttttcc
      781 ttcttctgta ctgtctttac tagtcacgac tccaggtgtt tgggcaagat acgccacttt
      841 cccaccttcg acttcttctg ctatcttctt agtaatcatg gacatcccta gatttgaatt
      901 tgcatctttc gaaagcacaa tcgaatctgc ctcccacttg tcgactgctg ttcgattatc
      961 ttgagctaag ccataagcta acccagtcaa aaaaaatacc aggtaggcga tcaaaaacat
     1021 cacccctgca actaacgcat aacgtaactt tgaatgcata atttcattta atgctaaaaa
     1081 catttaactc ctcctcctga aataatcatt ttatacaaca attttatcac ttgataaatt
     1141 tttggcgaat attatgctca aaaaattctt ccgctatttc attatatgaa ttttttattc
     1201 tcgattgatt aatatttatt atatccccac acaataaaag accgtgatat ttctcacagt
     1261 cttttatcta ctctattgct atttgctctc ttagctagta ctttaaaagc taactcattg
     1321 aggtagtgaa aacgagaggt gtattcagac agaaatccaa aatcctcaac tgctagctgt
     1381 ggaatcgtag ttttgtgttt cacgatatcc gcaagcaggt tcaaatctga taataagaat
     1441 tcttggctta tcagctgtcc gccaattatt tctaatgtat ccggatggta gatcagcttt
     1501 gc
//
LOCUS       DAYLZO010000269         1462 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.285, whole genome shotgun sequence.
ACCESSION   DAYLZO010000269 DAYLZO010000000
VERSION     DAYLZO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.285"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            273..>1462
                     /gene="asnB"
                     /locus_tag="ACGVJG_003175"
     CDS             273..>1462
                     /gene="asnB"
                     /locus_tag="ACGVJG_003175"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFJ6559052.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGR"
BASE COUNT          494 a          236 c          291 g          441 t
ORIGIN      
        1 cacgatcttt aggtcgtgtc tttttttgct ggtttttttg gaaatagcat ccactaatat
       61 ttttttaaca cgaagagaat aaggtagcct caaaaaagaa aacatggtaa attagatatt
      121 gggactttat tttctgtaaa aacagaaact ttcatctttt gttttcacgt gacgaaattg
      181 taagagagaa aatattcgtc cgctatttat tctatgataa gatgcatatg aatgaaataa
      241 acagtaattt tatattagga tgtgaaagtt aaatgtgcgg tattgtaggt tttgttaacg
      301 acaaggacaa caaaaaaaca attattaata caatgatgga tcgaatcgtt caccgtggac
      361 ctaatagttc aggagaatat atcgataaac atgttgcttt aggattcaga agattaagta
      421 ttattgactt agagggcggt actcagccta tttacaatga agatcggaca aagatcatta
      481 tttttaatgg cgaaatctat aattaccagc ctttgagaga agaattgatt gcagctggcc
      541 acgtttttca aacacatgca gatacagaag tccttttgca tggttacgaa gaatggggaa
      601 cagaattatt gcaaaagatc cgcggaatgt ttgcttttgc tatttgggat aatgaaaaga
      661 atgagttatt cggtgcaaga gaccactttg ggatcaaacc atattattat gcagaaatga
      721 acggaacatt tatgtttggt tcagaaatca aaagttttct gcctcatcct gattttaaca
      781 aagaattgaa caaagaagcg ttgaaacctt atatgacttt ccaatattct ccattaaacg
      841 gcgaaacatt cttcaaagat gtctatcgtt tgcctgaagg ccattattat acgtataaag
      901 atggaaaaat agacattcag caatattggg atgcagattt tgaaacaaaa gaaacgcatt
      961 cccgacaaga atggatcgaa aaaatcgacg aaaccgttca agcttctatt gaagcacata
     1021 cggtcagtga tgtagaagtt ggttctttct tatccagcgg ggttgattcc agttatgtaa
     1081 cctctgtatt gaaaccagat cattctttct caatcgggtt tgatgataaa acctataatg
     1141 aagcgattga agccagaaaa ctaaccgaac tattggactt agataataca gcagcagtga
     1201 tcgacggaga catgtcgttc aaagcattcc ctttgatcca gtatcactta gatgaacctg
     1261 attcaaatcc ttcttgcgta cctttgtact ttttagcaaa tcttgcttca caaagtgtgc
     1321 gggtagtcca atctggagaa ggggcggatg aactgtttgc gggataccaa acgtatggat
     1381 tccatacgaa ttcaaaattc atccgtgtga ttgctcaagg attgaaaaaa ttaccaaaag
     1441 gaacacgcta taatttagga cg
//
LOCUS       DAYLZO010000270         1457 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.286, whole genome shotgun sequence.
ACCESSION   DAYLZO010000270 DAYLZO010000000
VERSION     DAYLZO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.286"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(100..1119)
                     /locus_tag="ACGVJG_003176"
     CDS             complement(100..1119)
                     /locus_tag="ACGVJG_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFJ6559053.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1130..1336)
                     /locus_tag="ACGVJG_003177"
     CDS             complement(1130..1336)
                     /locus_tag="ACGVJG_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFJ6559054.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          403 a          304 c          246 g          504 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgatc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcata tctatcctcc tatttatctg
     1141 atgaaatttt cttatacgtt cgatttgata cacctaaaat actccctaga aatgctccaa
     1201 aacctgtaat aatcatcacc gttatatctg tgtaccgcca gttaacacct tttccgacaa
     1261 gaccgacaaa agtcgctaaa gcggggataa ctactatagc gatccatttc aaaatatcaa
     1321 aggttctatt gttcatacta tccacctccc ttcattggtt gggctacttt ctttttcaat
     1381 cgcttgtttt cttctatcaa tatttctctt tctgaaagca aggcatcaat ttgcccgtcc
     1441 agttgttcaa ttttatc
//
LOCUS       DAYLZO010000271         1445 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.287, whole genome shotgun sequence.
ACCESSION   DAYLZO010000271 DAYLZO010000000
VERSION     DAYLZO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.287"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            97..711
                     /locus_tag="ACGVJG_003178"
     CDS             97..711
                     /locus_tag="ACGVJG_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFJ6559055.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(735..1085)
                     /locus_tag="ACGVJG_003179"
     CDS             complement(735..1085)
                     /locus_tag="ACGVJG_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFJ6559056.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1079..1342)
                     /locus_tag="ACGVJG_003180"
     CDS             complement(1079..1342)
                     /locus_tag="ACGVJG_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFJ6559057.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          464 a          270 c          229 g          482 t
ORIGIN      
        1 taccctaact agacacgacc gcgacattga aaaatctggt tttctcgtgt atgattaggg
       61 tgttttttaa atagttgcag aaagtaggta aaaattttga gagttgcata catacgtgtc
      121 tcatccatcg atcaaaatga acaacggcag attgaagaaa tgaaaaaatt cggtgcagag
      181 agaattttca ttgagaaaca atctggcgca acgattactc atcgacctgt ttttcaagaa
      241 gcactggatt tcgtccgaga tcaagatatt tttattgtgg aagctattga tcgacttgga
      301 agaaattacg acgagatcat cgcttcagtc aattacttga agaagaaaaa tgtgcagctg
      361 atcatcacaa gtctaccgat catggccgaa gcaattggca atcctctcct ggataaattc
      421 attaaagatt tgatcattca aattttggcc atgatcgctg aacaagaacg tacagaatct
      481 aaacgacgtc aggcacaagg cattaagatt gcaaaagcga acggtgttta taaaggacgt
      541 cctaagctat acagtgccaa tgcaaaagat ccccaacgtc gtcttgttta taaaaacatc
      601 gttgaggatc taaaaaaagg tgttgcgatc gctaagattg caaaagacta caatgttacg
      661 agacaaaccg tatatagaat aaaaaaagac agcatggtca atcacgaata gttgatcatg
      721 ctgctgtttc tattttattt gcttactttt tgaaaaactg ttctaacttt catcaataac
      781 tcctcaacta aactatcatg cgctttttcc aaaaaaatac actctctggc ttcataatca
      841 attgtcgtta gttgatctaa taatactttt cctgttgtct tcatttcata ttctgagttt
      901 atatttacat atagcggata gtttctagtt gtactagaaa ttggagcaac tgtaacaacg
      961 ttactataat ccgcaataat atcatgactt aaaacaatat aaggtctttt acctgtctgt
     1021 tcgtgtccag atcgaggtgc tgtattcaat aatagaatat cgccttgtct aggttttctc
     1081 accatttttc attacctact gcttcaccta aattttgaat cgtcacatga tttctttcat
     1141 cagtgtaatt ttcaaataac tgttcaattg ttaattcatt ttctatatta gccgcttcga
     1201 taattagccg cttctgaccg tttacttctt cttgtattat cttcactttg tcttctgtat
     1261 gaattccaag atattccata atatactttg gcaatctaag gccattacta tttccccatt
     1321 tttttatagg taacatttcc atagtagaca cctctttctg tatatacata gtataacata
     1381 cataagaaaa aacgtatata cttaattatc cttttctatt aaattagatt aacattccat
     1441 gaatc
//
LOCUS       DAYLZO010000272         1424 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.288, whole genome shotgun sequence.
ACCESSION   DAYLZO010000272 DAYLZO010000000
VERSION     DAYLZO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.288"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(150..1337)
                     /locus_tag="ACGVJG_003181"
     CDS             complement(150..1337)
                     /locus_tag="ACGVJG_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFJ6559058.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAYLZO010000273         1402 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.290, whole genome shotgun sequence.
ACCESSION   DAYLZO010000273 DAYLZO010000000
VERSION     DAYLZO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.290"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            109..840
                     /locus_tag="ACGVJG_003182"
     CDS             109..840
                     /locus_tag="ACGVJG_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFJ6559059.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            975..>1402
                     /locus_tag="ACGVJG_003183"
     CDS             975..>1402
                     /locus_tag="ACGVJG_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFJ6559060.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTT"
BASE COUNT          485 a          237 c          295 g          385 t
ORIGIN      
        1 ttttatgaaa aagaccggtc aatcaagcgc tggttgttga tgaaatatat gttccataac
       61 tatattccaa gattcaaaaa gtattttcat gtgaaagaag gctagttcat ggagaatttt
      121 ttcagtattt taggcggaat gggcacgatg gcgacagaaa gttttgttcg cttgatcaac
      181 caccgaacga aagctacaaa agatcaagaa tatttgaatt atgtcttatt caatcatgca
      241 acagttcctg atcgtacagc ttatatttta gatcgatcag aagaaaatcc aatgccgttt
      301 ctgctggatg atattgagaa acaaaattta ttgcagccga acttcattgt gttgacttgt
      361 aacaccgcac actacttttt tgaagaatta caagcggcaa cggatattcc tatccttcat
      421 atgccaagag aagcagcaaa tgaacttgtc cgtcagcata cgacagggag agtagcgatt
      481 ctaggaacag aaggaagtat gaaagctgga atctatgaac gcgaagtcaa aaatcttgga
      541 ttcgaaacag tgattcctga tactgttctt caagaaaaaa tcaattatct gatctatcat
      601 gagatcaagg aatcggatta tttgaatcaa gagctatatt acgaaatctt agaagaagcg
      661 gtagagcgcc taaactgtga aaaagttatc ttaggttgca cagaattgtc attgatgcat
      721 gaatttgctg aagataacca ttatccagtg atcgatgccc aatctatttt agcagatcgg
      781 acgattgaac gagcgctagc tgaaagaagc aaggcattgg atacagcatc ggaaaagtaa
      841 aaaaattgga tttggggcaa atatcctaaa agatatttgg tccaaatctt tttgtaaaag
      901 aaaaagaaaa ctggtaatga tacgcccgga atgatataat tttactatgc acaatgtgag
      961 aggagttcgt aattatgaca gataatttgc aattagtcat cataacaggt atgagcggtg
     1021 cagggaaaac agtagctatc caaagttttg aagatatggg ctatttttgt atcgataata
     1081 tgccgcctcg attgattcca aaattctggg aattaatcaa agaatcaggc aaagtcacaa
     1141 aaatggcttt aggagtggat ttacgttctc gttccttctt tgaagaaatc caaaacatgc
     1201 tgatcgaaat cgaaaatacg aacttcatcg acacttctat cttattttta gatgcttcag
     1261 acgaagaact agtggctcgc tacaaagaaa caagacgagc acatccaatg gcgatggacg
     1321 gtttgatcac tgaagggata cgtaaagaac gggcgatact agacgacttg aaagcccaag
     1381 cttccgtgat cattgatacg ac
//
LOCUS       DAYLZO010000274         1390 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.291, whole genome shotgun sequence.
ACCESSION   DAYLZO010000274 DAYLZO010000000
VERSION     DAYLZO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.291"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1234
                     /locus_tag="ACGVJG_003184"
     CDS             <1..1234
                     /locus_tag="ACGVJG_003184"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFJ6559061.1"
                     /translation="TPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEV
                     EIPMGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLS
                     GKSEYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFL
                     VDVKDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLG
                     RNKEIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHA
                     YAARNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKE
                     LAQVIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREA
                     EKMNQQTAIQILEKIREK"
BASE COUNT          460 a          256 c          327 g          347 t
ORIGIN      
        1 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
       61 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
      121 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
      181 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
      241 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
      301 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
      361 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
      421 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
      481 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
      541 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
      601 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
      661 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
      721 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
      781 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
      841 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
      901 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
      961 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
     1021 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
     1081 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
     1141 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
     1201 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
     1261 gcaagattta cagttatttt atttactgca gatcttgctt ttttggctct tcgtcaaatt
     1321 gtgttggcga agacaaattt gaataaaact tagtccgata tgcggcgcgt tctgtgcgcc
     1381 gcatattttt
//
LOCUS       DAYLZO010000275         1376 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.292, whole genome shotgun sequence.
ACCESSION   DAYLZO010000275 DAYLZO010000000
VERSION     DAYLZO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.292"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..687)
                     /gene="msr(C)"
                     /locus_tag="ACGVJG_003185"
     CDS             complement(<1..687)
                     /gene="msr(C)"
                     /locus_tag="ACGVJG_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFJ6559062.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKV"
     gene            complement(1041..>1376)
                     /locus_tag="ACGVJG_003186"
     CDS             complement(1041..>1376)
                     /locus_tag="ACGVJG_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFJ6559063.1"
                     /translation="NQAYESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLA
                     AMQFMLVARDHGYETNPIGGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRL
                     APEKITMFE"
BASE COUNT          386 a          301 c          213 g          476 t
ORIGIN      
        1 cactttttcc aacttttccg cttgtttttt cttttcttgg atcgattttt ccaatcgttt
       61 tttctctttc atgatcgact ctgcttctcg tagctgagtc tttttctcca attccttttg
      121 agtgagatag gcactataat tccccgaaaa ctctcggatt tctccgtctt ttacttccca
      181 gattttcgat gctagctcat ctagaaaata gcgatcatga ctaacaaaaa ccagcgtacc
      241 ataataataa cgaagctctt caatcagtcg atcaatactt tttctatcta gatgggttgt
      301 tggctcgtct agaagcatcc cttgttcata agaagaaaga agacgtatca actcgatctt
      361 tcgttcttcc cctccgctcc atttccgctc tgaagtttct tccagtctta actggaaatg
      421 actggctaat tccaaattta ggtcctttgc ttcagcgaga taggttaatt gaggcaggta
      481 gttgaaggtg atctctgttt ggattttccc atgatcggga aaaagttcac cggctattag
      541 tttgagcaaa gtcgatttac cgacaccatt ttctcctatg atccctatac gatcttgctg
      601 atagacacga agagaatcaa tagatagttc tagctggttg ccaaagctga cttgtagatt
      661 tgttatattt actgctaaat tttccatatg ttttacactc ctatttcatg taaaacaaca
      721 attatcgata ctaaaaaaga gcagaaaaat ctgtttgcaa aaaaactgca tcacatcttt
      781 tcctactctg aacgaaataa ctatgactaa atcaccaaat ctgtcatagc tttcacctat
      841 tctactgtcg ttcgaaaaaa tggacatact ccatccatga aattgttgtt tatacatgtc
      901 ttttgaattt atttagttgt tattcaaaag ttcgaaacgt aatttcatcg atgcagtcat
      961 ttttccgacc tcctttttta ttaggttacc acacacgatt caaaaaaagc aatagctact
     1021 gaatagtaac tgaaaacctt ttattcgaac atagtgatct tttctggtgc taaacgaaca
     1081 gactcgtaac cttcttctac cgcttttcct attgataaaa taataactgg aacataacgg
     1141 tctttatcca aaccaaatgc ctctgccaac tgatctgctt cgaatccacc aattggattt
     1201 gtttcatagc catgatcacg cgcaactaac atgaattgca tagccgctaa gctagaatcg
     1261 actttgacta catcgttcat ttcttctctt gaaaaactct tgtaataagg aatgatcgca
     1321 gctaattgtt gatctctgac ttcttttggc atcttccctg attcgtaagc ttggtt
//
LOCUS       DAYLZO010000276         1369 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.293, whole genome shotgun sequence.
ACCESSION   DAYLZO010000276 DAYLZO010000000
VERSION     DAYLZO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.293"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..651
                     /locus_tag="ACGVJG_003187"
     CDS             <1..651
                     /locus_tag="ACGVJG_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFJ6559064.1"
                     /translation="GLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRF
                     HRRLLPEPVLVFMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKAT
                     LQEALPRYEITDVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGAD
                     HPVFTKSSLVQIAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            648..1367
                     /locus_tag="ACGVJG_003188"
     CDS             648..1367
                     /locus_tag="ACGVJG_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFJ6559065.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
BASE COUNT          476 a          259 c          275 g          359 t
ORIGIN      
        1 ggattagctc atgctgtgca aacagcgatt aaaccagacg gacgattcat ctacttaagt
       61 gccacacctg ataaaaagtt actaaaggaa atcaagcaga cgtttgacat ccacaaactt
      121 gctttgcgct ttcatcggcg tttacttcct gaacccgtct tggttttcat gaataattgg
      181 cgacaaaaat gcaagaacaa gaagaagata aaatctttgg tgcggatcat tcatcaatta
      241 attaaagaaa accatatcct tttattttgt ccaagtatca cgatgatgca tcgtttgaaa
      301 gccacacttc aagaggctct tccgagatat gagatcacag atgtttcttc aagagacaat
      361 cagcgggcgg aaaaagtcca gaaaatgcga gaagcaaaat atgacatact gttaaccacg
      421 atgatccttg aacgtggagt cacatttgaa agaatatccg ttatcgtttt aggagcggat
      481 catccggttt ttactaaatc ttctttggta caaatcgcag gaagagccga tcgtaaaggg
      541 ccttatacga acagtcaagt atactttttc tatgaagaaa aaactgcagc tattcgaaaa
      601 gcttgtcagg aaatcaaaga aatgaatgaa gaagggaaga agatgctgtg aggtgcagtt
      661 gctgcagtaa agatatcgtc caaaatttaa cattcagcga gattattggt gtttcgaaat
      721 acagattacc tcagacttgt tcagtttgtc gcgcacaatt tcatttattg gaaacaagag
      781 gctgttctgg ctgtcaaaaa ccaatccatt tcaatctaga gagtacgaaa aagaaagtaa
      841 agtctattcc cgtttactgc agtgactgtc aagaatggaa gaagaaatat ccgacctatg
      901 attttcagca ccgtgccttt tttgtgtacg atgaaaatat gaaggcctgg ctcaatcaat
      961 acaagtttat gggagatatc cgactagcag gcacctttgc atataaatgg acgcagataa
     1021 aaaaagaata cccgacatac atctattgtc caataccttt atctgaaaaa aggcgaacag
     1081 aaagaggatt caatcaagta agcgagatgt tgaaagcagc aagaattcct tttcagatgc
     1141 tactcaaaag agagaagcat ctgctggctc aagcaaaaaa cacgcgaaag gaaagattgg
     1201 agatgcctca accttttgaa cttgcagtca ccttttctaa gatacaaaat aaaaaaatat
     1261 tgattattga tgatgtctat acaacaggac agactctttt ccacgcagca gattgtttgc
     1321 aggtcgcaca gccgaaaatc atccgaacca tttctttagc aagatgaat
//
LOCUS       DAYLZO010000277         1368 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.294, whole genome shotgun sequence.
ACCESSION   DAYLZO010000277 DAYLZO010000000
VERSION     DAYLZO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.294"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            82..>1368
                     /locus_tag="ACGVJG_003189"
     CDS             82..>1368
                     /locus_tag="ACGVJG_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFJ6559066.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAYLZO010000278         1364 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.295, whole genome shotgun sequence.
ACCESSION   DAYLZO010000278 DAYLZO010000000
VERSION     DAYLZO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.295"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..401)
                     /locus_tag="ACGVJG_003190"
     CDS             complement(<1..401)
                     /locus_tag="ACGVJG_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFJ6559067.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDE"
     gene            complement(858..>1364)
                     /locus_tag="ACGVJG_003191"
     CDS             complement(858..>1364)
                     /locus_tag="ACGVJG_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase FAD-containing subunit"
                     /protein_id="HFJ6559068.1"
                     /translation="EYNVTDVASGASAAGDATTAASGAGASAGEAATQAAHFGLSYAY
                     GEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALALIAGLFTWLCSA
                     TTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPWLQRTLGNWWYGR
                     PSSQYGKK"
BASE COUNT          382 a          308 c          257 g          417 t
ORIGIN      
        1 tcttcgtcac ctccatctgt tgaacggact tcgatcacta atttatgtgg caaacagacg
       61 atggtttcgc catttttcgt tgcccagcct cgacgtacgc agatctggtc atcgcaatct
      121 gcttctttta tccgtactcg atcgccatct atctcgatca agttataatc tccgtgttca
      181 tctttgtaag tatatgtgtt gctctcttct ccagccatca aagggaatgt tttgatttct
      241 gtgccatcca cacgcagtac cgcttctttt tctgctgttg cggtgttttg ctgaagacca
      301 aaaatcatga ctggtaaaaa agaaagaaca gtcaaaagca aaataataaa aacatcccaa
      361 ggtttgagca gacttttttc agcaaattct ttaaggctca tttttgttct cctttttcca
      421 gattaactca cccaatttta gcataaaaac aagaggttgt gacacctgtg tcttgctttg
      481 agtaataaga acgaagaata gaaaacagct ttccatgttt tctaattctt tgggcttatt
      541 accgagaagc agcaggtgga acaccgttta taacaagagg ttgtgacaga agtgggcaac
      601 ttcaagaaat aagaaggaat ttacgaaaat tacttttcaa gtttttgtga aatttcggct
      661 tatttttcga agaagctact tctggaacac cgtttataac aagaggttgt aacagaggtt
      721 gtgacacctg tgtcttgctc atggaggctt atctcgggca tcttattcct ttaattttac
      781 ataaaaagga ggctgtgacg gtttgtctca gcccctttct attgaaatct tgcattcaaa
      841 taacgttctt tatctattta tttcttgccg tactgggaac ttggtctacc gtaccaccaa
      901 ttacctagtg tacgttgtag ccatggaatt acccagcgat ccaacccaat cgcacgtccg
      961 gaaccgttca tcaatgcgaa agcaacaaaa atgaaccaaa tgtttaccca gtaaaacatg
     1021 cctgataagc agaacgtgat caccaataca atcgttgtcg cactgcatag ccatgtgaat
     1081 aatccagcaa tcaatgctaa tgccaaggca atttcaaaga ataccatgaa tttttggaag
     1141 aacattgcta catcattgtt tggcatgatc acttgcatga tctttgtgaa ccagtctggt
     1201 gacttctcaa tcacagccat tggttcttca ccgtatgcat aactcaaacc aaaatgtgca
     1261 gcttgagtcg ctgcttctcc ggcacttgcc cctgctccgc tggcagctgt ggttgcatca
     1321 ccagcagcag aagcaccact ggcaacatct gttacattgt attc
//
LOCUS       DAYLZO010000279         1355 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.296, whole genome shotgun sequence.
ACCESSION   DAYLZO010000279 DAYLZO010000000
VERSION     DAYLZO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.296"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1355
                     /locus_tag="ACGVJG_003192"
     rRNA            <1..1355
                     /locus_tag="ACGVJG_003192"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYLZO010000280         1342 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.297, whole genome shotgun sequence.
ACCESSION   DAYLZO010000280 DAYLZO010000000
VERSION     DAYLZO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.297"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(329..>1342)
                     /locus_tag="ACGVJG_003193"
     CDS             complement(329..>1342)
                     /locus_tag="ACGVJG_003193"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6559069.1"
                     /translation="NSILLSPIINFLYALAIALALTLFGIDALHSPVEVGLIYAFTTY
                     VQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMDTEEYAPQQNPGADQAITSGKIEFRN
                     VSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEGQILIDDH
                     DIREYPMEELRKKMGLVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFVQANKFID
                     ELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTETEGLIQEG
                     LAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVERGNHETLLAQNGLYADMYKLQS
                     SGV"
BASE COUNT          372 a          313 c          242 g          415 t
ORIGIN      
        1 ctcctttcca tgatatatag ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc
       61 catggaacca tgggagactc atcaggttta gcttgcatca ggcaatcact atccaagttg
      121 tatcgttttc tttatattat cgaaagcaaa cgcttttgtc aacgctttct tagtggttat
      181 tagccgcttt tgactagctg tcgtcgtctt ttttaaatcg ggtgactacg ccactaacgc
      241 tcacagatga agaatctgcg aagctctgtt ttattttcac tctagcgctt agggcaaaat
      301 gctactatgc tactgtcaca acctcttttt acacaccact gctttgcagt ttgtacatat
      361 ctgcatacaa accattttgc gctagaagtg tttcatgatt cccacgctct actatatgac
      421 ctttgtctaa aactaaaatc aattctgcat cccgaatcgt ggacagccga tgggcaatcg
      481 ctatcgttgt ccgtccttgt cgcatttttg ccaatccttc ttggatcaat ccttcggttt
      541 cagtatcgat atttgctgtt gcttcatcca atactagtat tttaggatct gtcacgattg
      601 ttcgcgcaaa agtgatcagc tgtcgttgtc cactagaata gctggcaccg tgttcgatca
      661 cttttgagtg atatcctttt ggtaattcat cgatgaactt gtttgcttga acaaactcag
      721 ctgcatggcg aatctgttcg tctgtgatag ctggattcat catccggata tttcctgcaa
      781 tatctccata aaacaagaag gcatcttgca agaccaatcc cattttctta cgtaactctt
      841 ccattggata ttctcgaatg tcatgatcat caatcaagat ttggccttca taaaattcgt
      901 aaaaccgcat caatacgttg atgatcgagc tttttccgct tccggtgtgc cccactaatg
      961 caacggtctc acccggatta gcgacaaaag agatattgtt caatacattg tgctctccat
     1021 cataagaaaa gcttacattg cgaaactcga tcttacctga agtaatcgct tgatctgctc
     1081 caggattttg ttgcggtgca tattcttctg tatccatgat tttcaaaata cgactaccgg
     1141 caaccatccc atctgtaaag gtgctcaaaa agtccatcat ttgagacatt ggattaaaaa
     1201 attcctggac ataggtcgta aatgcataga tcagtccaac ttctacggga gaatgcaacg
     1261 catcgatccc aaacaaagtc aacgccaacg caatcgccaa tgcatagaga aaattgatga
     1321 ttggacttaa cagaatcgaa tt
//
LOCUS       DAYLZO010000281         1337 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.298, whole genome shotgun sequence.
ACCESSION   DAYLZO010000281 DAYLZO010000000
VERSION     DAYLZO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.298"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            306..>1337
                     /locus_tag="ACGVJG_003194"
     CDS             306..>1337
                     /locus_tag="ACGVJG_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFJ6559070.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADE"
BASE COUNT          434 a          218 c          299 g          386 t
ORIGIN      
        1 cctgtcatta agaaaacaaa tgagtgaaat aaatgctttc ttttcataat caacctccta
       61 aatttccaat attttattat tataggcgaa aagtgctgaa tcagacaaga ctgtaagctc
      121 gaaaaaatga aaaaaatgcg agtttctaac attttatgtt ataaaacgta acatgcataa
      181 taagataaaa aagtggcgaa agtatgttcg gtgtggtata attttttagt aaggaatgcc
      241 tttgctttcc ttttcttttt attggtcgta aaatgaaaag gaatgaatga aaaggaggac
      301 gaagaatgat tgagagagat acctcccgac actttgagct tgtctcgaaa taccaaccgg
      361 ctggtgatca gcctgaagcg attaaccaat tagtggatgg cgtagtcggc gggaaaaaag
      421 cacaaatctt attaggtgcg actggaacag ggaaaacata tacgatttcc aatcttattg
      481 agaaagtgaa taaaccaaca ctaattattg cccataacaa aacattggct ggtcagctgt
      541 acggggaatt caaggaattt ttccctaaca atgctgttga atattttgtt tcttattatg
      601 attattatca gcctgaagct tatgtgcctt caagcgatac gtatatcgaa aaagattcaa
      661 gtgtaaatga tgagatcgac aaattacgac attcggctac tagttcatta ttagaaagaa
      721 atgatgtgat cgtcattgca tccgtttcat gtatttttgg attaggttcg ccttttgagt
      781 accaaaaaca agtagtttcg attcgacagg gagcagaact agatcgcaat cagttgattc
      841 gtgacttagt cagtatccaa ttcgaaagaa atgatataga ttttcagcgc ggtcgttttc
      901 gagtgcgcgg tgatgtcgtg gagatttttc cggcttcaag agatgaacgt gccttacgtg
      961 tggagttttt tggagatgag gtcgaacgta tccgagaagt caatgcactg acaggtgaag
     1021 ttttaggaga aacggaacac gtggcaatct tcccggcgac tcactttgtg acgaatgatg
     1081 aacatatgga gcatgccgta gcgaatatca aagcggaatt agaacagcgt ttaactgtct
     1141 tgcgaaatga aaacaaactt ctggaagctc aacgtttaga acagcgaacc aattacgata
     1201 ttgaaatgat gttagaaatg ggctacacgt cgggaattga aaattattct cggcatatgg
     1261 acggacgtaa agaaggtgaa ccgccttata cgctattgga tttctttcct gaagattttt
     1321 tgatcgtggc ggacgaa
//
LOCUS       DAYLZO010000282         1313 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.299, whole genome shotgun sequence.
ACCESSION   DAYLZO010000282 DAYLZO010000000
VERSION     DAYLZO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.299"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            96..>1313
                     /locus_tag="ACGVJG_003195"
     CDS             96..>1313
                     /locus_tag="ACGVJG_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFJ6559071.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNA"
BASE COUNT          429 a          270 c          239 g          375 t
ORIGIN      
        1 accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat cctttagaat
       61 taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag
      121 aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa
      181 aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat
      241 gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc
      301 ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa aagcaacgct
      361 tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg aaaaaacatt
      421 gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa
      481 tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt attatgaagc
      541 gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt tgttttgatg
      601 aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg gatggacaaa
      661 cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa cgttatttta
      721 gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat atgtacgctc
      781 cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt gaccggtttc
      841 acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa acgaaccaac
      901 tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa agatactgga
      961 aacttcttca aaaagatgaa cgcaagcttg attatactcg acgcctttgg cgacctggct
     1021 tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaggg agccctgctt
     1081 tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa cgagactacg
     1141 tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat cagacgacct
     1201 tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa agttattcaa
     1261 atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat gca
//
LOCUS       DAYLZO010000283         1313 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.300, whole genome shotgun sequence.
ACCESSION   DAYLZO010000283 DAYLZO010000000
VERSION     DAYLZO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.300"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            200..742
                     /locus_tag="ACGVJG_003196"
     CDS             200..742
                     /locus_tag="ACGVJG_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559072.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1313
                     /locus_tag="ACGVJG_003197"
                     /pseudo
     CDS             898..1313
                     /locus_tag="ACGVJG_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
BASE COUNT          532 a          148 c          256 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgt ggaatggcaa
     1261 cacttttttt gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gct
//
LOCUS       DAYLZO010000284         1299 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.301, whole genome shotgun sequence.
ACCESSION   DAYLZO010000284 DAYLZO010000000
VERSION     DAYLZO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.301"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            103..1290
                     /locus_tag="ACGVJG_003198"
     CDS             103..1290
                     /locus_tag="ACGVJG_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFJ6559073.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
BASE COUNT          482 a          195 c          238 g          384 t
ORIGIN      
        1 tcgtaaatag ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaagtgg
       61 caacattgtg tacttttctt tgcaatcttg tgtacaaaac tattgcaaaa cacagcgcat
      121 aagaatcctt acaaaacagt agatgaagca acccaattag atcttgttat agttgttaat
      181 cagctcttaa cgggttacga ttcaaaatgg gtcaacaccc tatacttaga taagtcactt
      241 gaatatcaga acataattca ggctttttca cgcacaaatc gtcttaatgg tcctgaaaaa
      301 ccgttcggaa ccattcgtta ttatcgtaaa gcacatacga tggaacagaa cattgaagag
      361 gctattgagt tatactcagg caaacaagca aaagggttat ttgttgaaaa attatataat
      421 aacttagaaa agttgaacga tatattcgaa gaaattaagt ccgtatttaa tatttctggc
      481 atagaaaact tcagtcattt accggaggtt ccaggggaaa ttaagcaatt tgtaaatctt
      541 tataatcaac taatccattg ttatgaatca gcgatgatac agggattcac atgggcaaaa
      601 tcgacttatg aaatccagga ttatgaacct ataacagtag taatcacaga atatgaattt
      661 aatatcttgg cacaacgtta cagcgagata gagagagaac ctagagacgg aaatgatagt
      721 atagcgtatg acattgatta ccaattacct gaaggtagtc gtagtcgtat taattatgag
      781 tatttaaaca caaacttcaa aaaatacatg aatataaaag aagaacgtga ttcgaattta
      841 attcaatcat tgttaaacga cttacatcgc acatttgcgc aattaccagt ggagttacag
      901 ccatatgctg agatgattct aaatgatgta aataatgggg aattagttat caaagaagga
      961 tgggaattta ctgattacct caatgaatat cagcttagtg aagaggacga ttttatccaa
     1021 gatttagtgg attcaaccaa tattaatgag gcattattac gtgagatgtt ggatttacgc
     1081 ttaaccgagg taaacattaa tgaatatagt cgatttgata aattaaaatc aagtgttaac
     1141 gtaggtcatt tcagtggata tatcgagaaa aatttaggta aaatggtaat accaatcaag
     1201 gtaaatatgc taattgacag attattgcgt gcatttttat tagaagatgt attcgatgtt
     1261 acagagtaca taaagaatta ctttaactag agtacacct
//
LOCUS       DAYLZO010000285         1294 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.302, whole genome shotgun sequence.
ACCESSION   DAYLZO010000285 DAYLZO010000000
VERSION     DAYLZO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.302"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1244
                     /locus_tag="ACGVJG_003199"
     CDS             <1..1244
                     /locus_tag="ACGVJG_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFJ6559074.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAYLZO010000286         1282 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.303, whole genome shotgun sequence.
ACCESSION   DAYLZO010000286 DAYLZO010000000
VERSION     DAYLZO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.303"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1253
                     /gene="sufB"
                     /locus_tag="ACGVJG_003200"
     CDS             <1..1253
                     /gene="sufB"
                     /locus_tag="ACGVJG_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFJ6559075.1"
                     /translation="FRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWE
                     DVPEKIKDTFEKIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALK
                     EYPELFKEYFSKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENT
                     GQFERTLIIVDEGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDN
                     VYNLVTKRAKAYEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQ
                     DTGAKMIHNAPNTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEW
                     SKSDTIPFNEIHNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFT
                     KELPMEYAVELNRLISYEMEGSVG"
BASE COUNT          442 a          288 c          259 g          293 t
ORIGIN      
        1 aattccgtct gaaatctcta gaaacattta acaaaatgcc aatgcaaaca tggggccccg
       61 acctttcaga catcgatttc gatgccatca aatattacca aaaaccaagt gacaaacccg
      121 ctcgtgactg ggaagacgta ccagaaaaaa tcaaagatac atttgaaaag attggtatac
      181 cagaagctga acgagcttat ctagccggtg cttccgcaca atatgaatca gaagtcgtgt
      241 accataatat gaaagatgaa ttcgaaaagt taggaatcgt ctttactgac actgactctg
      301 ctttgaaaga ataccctgaa ttattcaaag agtacttttc aaaacttgta ccgccaaccg
      361 acaacaaatt agcagcgctg aattcagccg tatggtcagg tgggaccttt atttacgtgc
      421 caaaaggcgt caaagtcgat gttccattac aaacgtactt ccggatcaat gcggaaaaca
      481 cggggcaatt cgaacgtacg ctgatcattg ttgacgaagg agcaagcatc cactatgtcg
      541 aaggttgtac ggcacctact tattcaagta acagcctgca tgccgccatc gtagaaatct
      601 ttacaagaaa agatgcgtat tgccgttaca caacgatcca aaactggtca gacaatgtgt
      661 acaacttagt aaccaaacga gcaaaagcat acgaaggcgc aaccgttgaa tggatcgacg
      721 gaaacctagg tgcaaaaaca acgatgaaat acccaagtgt ttacttagat ggaaaaggtg
      781 cacgaggcac catgctctcc atcgcctttg caggagcaaa ccaaatccaa gatacgggtg
      841 caaaaatgat ccacaatgca ccaaatacat ctagctctat cgtctctaaa tcaatttcaa
      901 aagacggcgg agaagtaaac tatcgaggac aagtaacctt tgccaaaaac agtgcgggtt
      961 cgatctctca tatcgaatgt gacacgatca tcatggacga atggtcgaaa tccgatacga
     1021 tcccattcaa tgagatccac aacagccaag tctctttaga acacgaagcc aaagtctcaa
     1081 aaatctcaga agaacaactc tactatctga tgagccgagg cctgaccgaa tctgaagcaa
     1141 ctgaaatgat cgtcatgggc tttgttgaac cattcaccaa agaacttcca atggaatatg
     1201 cagtcgaact caatcgattg atcagttatg agatggaagg gtctgtaggt taataactat
     1261 ttcattgctc gaaacgttga ta
//
LOCUS       DAYLZO010000287         1281 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.304, whole genome shotgun sequence.
ACCESSION   DAYLZO010000287 DAYLZO010000000
VERSION     DAYLZO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.304"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..650)
                     /locus_tag="ACGVJG_003201"
     CDS             complement(<1..650)
                     /locus_tag="ACGVJG_003201"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HFJ6559076.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTF"
     gene            complement(678..1070)
                     /locus_tag="ACGVJG_003202"
     CDS             complement(678..1070)
                     /locus_tag="ACGVJG_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFJ6559077.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          366 a          282 c          194 g          439 t
ORIGIN      
        1 ttgaaggttt ggatattatc acaagcatct accacatctg catatttaaa gtcaatttca
       61 tgctgtcctg gcgctacttc atgatgtgaa gcttccactt caaagcctag actttctaat
      121 tccaacacga tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa atcaaaataa
      181 ccacctttat cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt aaataagaag
      241 aattctggtt caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat atctttcaat
      301 gctcgtttca aattcccgcg aggatcgccg gcaaatggtg tcccatccgg attatagata
      361 tcacaaatta aacgagcaac ttttccatga gcactttccc acgggaagat catccaagtg
      421 gagagatctg gatacaaata catatcgctt tcttcaattc gaacaaatcc ttcaatcgaa
      481 gaaccgtcaa acatcatttt attttctaat actttgtcga tctggctcac tggaacttct
      541 acatttttga tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg tacgttttct
      601 tcctcaatga ttcgtttgat ttcttcacct gttatctgtt tctttaccat ggtatccctc
      661 ttcctttttt aatttattca gtcaaagaat tgtctttgca atccttgcga ctgataatga
      721 cctgttttgc ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg tcgaacgtca
      781 tgatctgtca acggcttctt gttttttgct tcttgttttt tcgcctgtgc catttcgtat
      841 acacgtttga tcccagccat attcaatccc tctgaaagat agtctttaat ctcaagcaac
      901 atatcaatat cattcagcga atacatacgt cggtttcctt cgcttcgttc cggatgaact
      961 aaatcttgtt cttcataata gcgaatttgc cgagcagaca aatcagtcag cttcataact
     1021 gtaccaattg gaaatacgga catagatcgg cgcaattcct tttctcccat cagacgtccc
     1081 ctttcgtttt ataagttggc taaagtgtac caattaaaaa aaatattgtc aactgcatat
     1141 gttaggtttt ctaacatgag atctctattt tttataaaat aacaaaaaaa taagaacaca
     1201 aaaaaggtat ctagttgatt ttcgtaccag atacctttta ttttttatta tactgtacta
     1261 tcctttatta gtgtttttat t
//
LOCUS       DAYLZO010000288         1268 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.305, whole genome shotgun sequence.
ACCESSION   DAYLZO010000288 DAYLZO010000000
VERSION     DAYLZO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.305"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            4..273
                     /locus_tag="ACGVJG_003203"
     CDS             4..273
                     /locus_tag="ACGVJG_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559078.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            278..442
                     /locus_tag="ACGVJG_003204"
     CDS             278..442
                     /locus_tag="ACGVJG_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559079.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            435..737
                     /locus_tag="ACGVJG_003205"
     CDS             435..737
                     /locus_tag="ACGVJG_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559080.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            734..895
                     /locus_tag="ACGVJG_003206"
     CDS             734..895
                     /locus_tag="ACGVJG_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFJ6559081.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            892..1197
                     /locus_tag="ACGVJG_003207"
     CDS             892..1197
                     /locus_tag="ACGVJG_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559082.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT          488 a          197 c          306 g          277 t
ORIGIN      
        1 aaattgaaaa tcgattatct agaactaatt aatgaaatag caaagtataa aactggtgag
       61 gaaatagaaa tcctgagaga cgtatatgat caactcgaag aagctggaat cgaaggaatt
      121 aagaatgatc gttcgagttg gagtaagctc agatactatt tcgcacttta tatcgataca
      181 acacaattaa gaaatttagc atataccaaa ttactatttg ttgattgtgt taaaggcttg
      241 caaaaacatc ttaatgaact tgagcaggtg taatcagatg gacctaaaga catttacagc
      301 acagattgaa ctaatgcatc aagaagcttt aagacaaagt gtatcgtacg aagacaagtg
      361 gctcaacacg ttccacggcg gacgtgagag cgcacttgat caagtactca aattactgaa
      421 aggagaatgt cgggatggat aagaaagcgg caatgcagcg aattatcgaa ttgacttatt
      481 cagaagattg gcaaaatgac aaagaagctg cttcagaagt gatgaggctt ggaagagcga
      541 tgtgggcaga caagagcaac aagccaagac cacgaaaaat cgcaatttgg cacggtgaca
      601 aacttctagt gatagggaca gctgaacagt tagcaagtct cacaggcttg cacgagaaaa
      661 tcgtgaggaa aagagcaagg tgtggctaca cagacgctaa gaagagaacg tttagatacg
      721 tggaggaatc atcatgacaa cagaagaagt gattcaaatg cgtattcgaa gccttcagcg
      781 tgagattgac gatctggaac gaacaaaggc agtgatggtc aatgaaacgg cgagaaaggc
      841 aatcgatttg cacatagaga atttaagaag ggaaatccat cgattggagg aatgagcgtg
      901 gataagaaag cagcaatgaa aagaattgct gaattaacca agtcagaatc ttggcaagaa
      961 gacaaagaaa tagttgcaga agtccaaaag ctcggtaaat caatgtggac tgaaaagccc
     1021 aaacggagaa cgccgagaaa aattgcaatc tggcatgatg accgaatttt agtaacaggt
     1081 actgctgaac agttatctga aattactgga ttaagcaaaa acattatctg ggatagagct
     1141 aggagcttat ggattgattc aaaaggacga cagtttaggt atgtggagga gagataatgg
     1201 atctcattac acaatacagt gacatcatcc tcaagaaaat catgatgaag attcagaaag
     1261 ataaaaaa
//
LOCUS       DAYLZO010000289         1202 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.306, whole genome shotgun sequence.
ACCESSION   DAYLZO010000289 DAYLZO010000000
VERSION     DAYLZO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.306"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            189..338
                     /locus_tag="ACGVJG_003208"
     CDS             189..338
                     /locus_tag="ACGVJG_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559083.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            335..781
                     /locus_tag="ACGVJG_003209"
     CDS             335..781
                     /locus_tag="ACGVJG_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559084.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            783..920
                     /locus_tag="ACGVJG_003210"
     CDS             783..920
                     /locus_tag="ACGVJG_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFJ6559085.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            958..>1202
                     /locus_tag="ACGVJG_003211"
     CDS             958..>1202
                     /locus_tag="ACGVJG_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559086.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLV"
BASE COUNT          430 a          193 c          254 g          325 t
ORIGIN      
        1 aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg
       61 ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag
      121 aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt
      181 gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg
      241 tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg attccaaatt
      301 ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg
      361 tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac
      421 agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca
      481 aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga
      541 tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca
      601 gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa
      661 agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa
      721 tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta
      781 gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttc actaacgaac
      841 aagtaatggt tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa atcattagca
      901 aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg
      961 attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt
     1021 ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa
     1081 gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg
     1141 atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatgc agaaaaattg
     1201 gt
//
LOCUS       DAYLZO010000290         1194 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.307, whole genome shotgun sequence.
ACCESSION   DAYLZO010000290 DAYLZO010000000
VERSION     DAYLZO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.307"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1076
                     /locus_tag="ACGVJG_003212"
     CDS             <1..1076
                     /locus_tag="ACGVJG_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFJ6559087.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tctt
//
LOCUS       DAYLZO010000291         1174 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.308, whole genome shotgun sequence.
ACCESSION   DAYLZO010000291 DAYLZO010000000
VERSION     DAYLZO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.308"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            175..519
                     /locus_tag="ACGVJG_003213"
     CDS             175..519
                     /locus_tag="ACGVJG_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFJ6559088.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ACGVJG_003214"
     CDS             520..939
                     /locus_tag="ACGVJG_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFJ6559089.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
BASE COUNT          428 a          182 c          257 g          307 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gata
//
LOCUS       DAYLZO010000292         1157 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.309, whole genome shotgun sequence.
ACCESSION   DAYLZO010000292 DAYLZO010000000
VERSION     DAYLZO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.309"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>1157
                     /locus_tag="ACGVJG_003215"
     CDS             <1..>1157
                     /locus_tag="ACGVJG_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFJ6559090.1"
                     /translation="TKEKYKGQICLIYKGTLLYKPEECSHCLCVVPSRIIRWGTTTVR
                     LLLNDVSEYRTYLELKKQRFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENT
                     SMTEIARQKNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMM
                     DGQSHALLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQL
                     IIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYE
                     KRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDI
                     SGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGF"
BASE COUNT          352 a          232 c          234 g          339 t
ORIGIN      
        1 taactaaaga aaaatacaaa gggcaaattt gcttgattta caaaggaact cttctgtaca
       61 agccagaaga gtgttcccat tgtctttgtg tcgtcccctc taggatcatt cgttggggga
      121 cgacaaccgt tcggttgtta ctgaatgatg tgtctgagta tcgcacttat ctggagctga
      181 agaaacaacg atttaaatgt aagtcttgcc aaagaacatt cgtagcggat acctctgttg
      241 ctgaaaaaca ctgtttcatt agtcaaaaag ttcgttggtc tgtcattgct cgcttaaaag
      301 agaatacttc catgacggaa atcgctcgtc agaaaaatat ttccacttct tcggtctatc
      361 gtgtcatgaa gcgattctac cgcccattga atcctttcaa acagactttg ccaaaggtct
      421 tatgctttga tgaattcaaa tcggttcgaa gtgttgccag tgcaatgagt tttatcatga
      481 tggacggaca aagccatgca ttattggata ttgtcgaaaa tcgacgcttg ccttatttag
      541 aacgctattt ttctcggttt tcattagcga ctcgtgaagc cgttcaattg attgttattg
      601 atatgtatgc gccttatgtt tcgttggtga aaaagctttt cccgaatgct cagttgatca
      661 tcgatcgttt ccacatcgtc caacatattg gcagaacttt cttgaatcat cgagtgaaag
      721 aaaccacggt acgacttaaa ggaaattccc actctcaacg agggttaggg aaaaaattga
      781 agcgctattg gaaactccta caaaaagaag aagcaaagtt aaactatgaa aaacgggttt
      841 ggcgtgccag ttttaaagat tatttgactg aaagtgagat tgccgatcga ctgttagctg
      901 cttgtcccaa cttacgccaa ggctatcagt tataccaaga cattctttat gcggtaaaga
      961 aaagagatca gtcactattt gaggattgtt taactagaga cattagcggt ctgccagaaa
     1021 cctatacaac cactttacga acgtttaaaa agtttctgcc acagatccaa aatgcgcttc
     1081 actacagcta ttcaaacgga ccattggaat gcttaaacaa ccacatcaaa gtattaaaac
     1141 gcaatgcgta cggcttt
//
LOCUS       DAYLZO010000293         1138 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.310, whole genome shotgun sequence.
ACCESSION   DAYLZO010000293 DAYLZO010000000
VERSION     DAYLZO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.310"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..734)
                     /locus_tag="ACGVJG_003216"
     CDS             complement(<1..734)
                     /locus_tag="ACGVJG_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFJ6559091.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIG"
BASE COUNT          351 a          225 c          165 g          397 t
ORIGIN      
        1 ccaatcacac ctagtctatt cttaatcgta taaggattcc agttagcaag atctctaatg
       61 ctcataatcc ccatctgatt gagtcttttt ttcattcgag agccaattcc ccaaaagtcc
      121 gtcatttcag gaatattcca tactttttca ggaacgtttt cgtacgtcca ttccgcaaca
      181 aatccttcat tatgctttgc ttcgttgtct aaggctagtt tagccaaaag gggattatct
      241 ccgacaccta ctgtagcaat caatcctagc tcttctttaa tattctcctg gatcatttga
      301 gccagcttct ttctccgttg acttcgtgtt ccttcagtcg taaaaagatt cagtgatcgg
      361 gtcactttta agattgattc atcgatcgag taaatcagta gatcttcatc agccacatat
      421 cttctgaaaa tattatttac ctgcatattt cgcttgatat ataggttcat acgtggtgga
      481 acaacgtgta gtgttttagg aaatggttgt ggtaagtcac gtggtctact cacatttgta
      541 ataccatacc gcttttttgc ttcaggagaa gaagctaata tcaatcctga accagtatta
      601 tcagctcgac tcataacaac aagttctgtt gttaatggat ctaaatttct ttctatacat
      661 tcgacacttg cgtaaaagct tttcatatcg attaaaaagt aatcatttac tggctctttt
      721 gaataatcca tcataaataa cactccattc tttcacatta tacaaacata tgttcgatat
      781 tgtaaagaag aaatagctaa atacataaaa aaagaagcat aggtatgcta tcaccctgct
      841 tcttcttttg atttgtatga tcaatttatt ctatcacttt actaattaat ttgaaaatct
      901 aataaaaact ctcactctaa aagagtgaga gactgcaaat tagagtacgg acttttttgg
      961 agttccgttc tttaatatta acaaatgaat aaaatcctta caacatttga gaataagtta
     1021 tcagtagaca cttacaaaaa attctcatac tatttcctcc atcattagtt tttgtctata
     1081 taaggtttac cctaaataga cactggtata acattgaaaa atatggcttc ctcgtgta
//
LOCUS       DAYLZO010000294         1126 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.311, whole genome shotgun sequence.
ACCESSION   DAYLZO010000294 DAYLZO010000000
VERSION     DAYLZO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.311"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..793)
                     /locus_tag="ACGVJG_003217"
     CDS             complement(<1..793)
                     /locus_tag="ACGVJG_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6559092.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT          373 a          230 c          168 g          355 t
ORIGIN      
        1 aatacaacca acaaaactcc tcttctgata aagcaacttg ataatttgtt tgaatcgctt
       61 ctttcaagga atacattaag cgaaaattct cactagcaat tactcgctca atcagacctt
      121 gaggaatggc tatcgttttt ccttgtctca cacgctcaat cgcaatatac aaactataat
      181 aaaattcagc aggagaagtc cctgttccaa tcgagaaata ctcaaattta tcatagaatg
      241 tagcttgcac aagttcatga agatcccttg gtggaacaag agtatggggc gtcatctccc
      301 cttcataata gaagtcttta aaaaacttgc gaatatttgc ctcttctcct tttaaatcaa
      361 gaggataaag actcaattct aagccgtatt cttccaaaac tggttgaatc tgcttcaaat
      421 accgacgaaa agtcgcttca gataaatgaa aacgatcaat tagctccgtc atatcttcca
      481 attcgccata aaagacattc cctaacaact caaataaaat ttcgttctcc agtagttgct
      541 ccttctgctt gagaaaaaga accggtcttt ccttctgaaa agaataccca cttttcgttg
      601 aaacaaattg aacactttca gaaaagtagt ccttcatttt attgacatct gtttgaatgg
      661 ttcgttcaga tacatgatta ctctttgcta attctccttt tgtggcaagt tgattgcgct
      721 caaattgatt gataatccga aaccagcgta caatcgatgg atcaacaata aattttaact
      781 gtaaattttt catcaacaac cctcctctta gaaaaaaatt tcatcctata cctatataat
      841 tacgatacca ttttttctcc tttataggta atgttttggg gaaggaaaac aagtttctat
      901 tactcacctg ataaaatcaa taccgtcgac tatactgcgg aattgtttgg gtatagcaaa
      961 taagaccaaa tgaaaaaact tggtcttgga taaaaaatgt aaccgatttc tatttaccca
     1021 aaaagaggat atgctatact taatgagaca acttactatc attcaggtgt agtattgttc
     1081 ggtcgtggta ccccaacaac tttacaattt cttttgaaac gttggt
//
LOCUS       DAYLZO010000295         1122 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.312, whole genome shotgun sequence.
ACCESSION   DAYLZO010000295 DAYLZO010000000
VERSION     DAYLZO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.312"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..203
                     /locus_tag="ACGVJG_003218"
     CDS             <1..203
                     /locus_tag="ACGVJG_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6559093.1"
                     /translation="SIVVPRQIAMYLARELTDNSLPKIGAEFGGKDHTTVIHAHEKIQ
                     QLMDSSVSMQNEVSEIKNFLLN"
     gene            401..>1122
                     /gene="dnaN"
                     /locus_tag="ACGVJG_003219"
     CDS             401..>1122
                     /gene="dnaN"
                     /locus_tag="ACGVJG_003219"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFJ6559094.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSR"
BASE COUNT          377 a          204 c          189 g          352 t
ORIGIN      
        1 aatctattgt tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt
       61 ctttaccgaa aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc
      121 atgaaaaaat ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa
      181 tcaaaaactt tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata
      241 aaagaaaaaa acgttcaaaa gttatgcaca ctttttccac agctgttttt tcgtttctct
      301 cttacgtttt ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt
      361 aactaataat aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg
      421 agctagcttt atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat
      481 ccctattttg acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag
      541 caacgctgat atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca
      601 aatcgaatct actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag
      661 acttcctgaa gaaacattta ctttagaagt tttagaaaat aaacaagtag cgattacttc
      721 tggaaaagcg aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt
      781 tgtcgaaagc cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga
      841 aacagttttt gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat
      901 tttatctgat aattctttat tggctgtagc tactgattct caccgtctaa gtcaacgcgt
      961 gattccagta gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat
     1021 cgaattatct cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa
     1081 ccaagtgttg ttcaaaacag aaacgatgta tttctattcc cg
//
LOCUS       DAYLZO010000296         1120 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.313, whole genome shotgun sequence.
ACCESSION   DAYLZO010000296 DAYLZO010000000
VERSION     DAYLZO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.313"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>1120
                     /locus_tag="ACGVJG_003220"
     CDS             <1..>1120
                     /locus_tag="ACGVJG_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559095.1"
                     /translation="LGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQPMNDKRNGRSWF
                     NQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDMTIENIPRIREEI
                     ERFEKGESSFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAIIDSGRVTQWKKN
                     PTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKVAVRELLEEAAPTDSLKEKEND
                     HFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEV
                     VDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQK
                     GYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELL"
BASE COUNT          476 a          183 c          243 g          218 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaatga acaaatcgaa
       61 aagatgtttg aacaccaaaa aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc
      121 cgttcatggt ttaaccagca agaacgaatc gaaaataaag tccaaagtct gagagaagag
      181 atcaagcaac aagaaaaaca ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg
      241 ggttataaca agtacggcgg actagatatg acgatcgaaa atattccaag aatcagagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg tctttttcag ccgccacgat ccgaaaatat
      361 caacggaagt tggaaacatt agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat
      421 atcctccctg aagtacaagc gatcattgac agcggacgag taacgcaatg gaaaaaaaat
      481 ccgaccattt attttctgaa aggctatcga aaagtcgctt tagaactgaa tgaaaacggg
      541 gaatttgagg aatctaaaaa ataccaagcc acatcggaag aggaaaaagt agctgttcgt
      601 gaattacttg aagaggctgc gccaactgat tctctgaagg agaaagaaaa cgatcatttc
      661 tatcgagtag aattcaacga aaatgatcca gaaaaaggat tgaaaagtta tggtggagag
      721 atcgtaacat tagaattgat cgatcaactc aaaacgttgg acaaccaata cgctgcagaa
      781 gcaggttatt ataaattcta ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc
      841 cgaatggatc taggggatgg cgtcaacagt aatcaaccca tctatttaga gttagcaaaa
      901 ttgtgcgaac aagtacctga gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg
      961 aaagaacaaa tgcagtcgaa agaaacaaaa acaaaacaaa agggctatca aaaacgaacg
     1021 gcagaagaga ttaaacaaga agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa
     1081 aagcataccc attctcctga agacgtcaaa gaacttttga
//
LOCUS       DAYLZO010000297         1106 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.314, whole genome shotgun sequence.
ACCESSION   DAYLZO010000297 DAYLZO010000000
VERSION     DAYLZO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.314"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..1087
                     /locus_tag="ACGVJG_003221"
     CDS             <1..1087
                     /locus_tag="ACGVJG_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6559096.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DAYLZO010000298         1104 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.315, whole genome shotgun sequence.
ACCESSION   DAYLZO010000298 DAYLZO010000000
VERSION     DAYLZO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.315"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..366
                     /locus_tag="ACGVJG_003222"
     CDS             <1..366
                     /locus_tag="ACGVJG_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFJ6559097.1"
                     /translation="SSFNTEVEFSVPSFLAAIERASLLSHEGRNNIVRLSIRPDAVVL
                     YGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKAALRAFGDMSIKVKFISAIRPFTL
                     EPTEDGVQFIQLITPVRTN"
     gene            595..837
                     /gene="yaaA"
                     /locus_tag="ACGVJG_003223"
     CDS             595..837
                     /gene="yaaA"
                     /locus_tag="ACGVJG_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFJ6559098.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            824..>1104
                     /locus_tag="ACGVJG_003224"
     CDS             824..>1104
                     /locus_tag="ACGVJG_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6559099.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKT"
BASE COUNT          376 a          169 c          228 g          331 t
ORIGIN      
        1 tcaagtttta atacagaggt tgaattttct gttcctagct ttttagcggc gatcgaacgg
       61 gcttctttac tttctcatga aggccgtaac aacatcgttc gtttatctat tcgtccagat
      121 gcggttgttt tatacggaaa ttcgccagaa atcgggaaag tagaagaaag cttgagttat
      181 acagcaagca gtggcgatcc attagatatt tctttcaatc cggattatat gaaagcagcg
      241 ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta tctctgctat tcgtccattt
      301 acattggaac caacggaaga tggcgttcaa tttattcagc tgattactcc tgtacgtaca
      361 aattaacaat gattttcatt gttcttgaaa atttaattga aaagaaatag aaaaaataaa
      421 attttaaaca gaaggcccta aaatctcttt taaggctttc tgtttatttt tgtttaaatc
      481 acctttttta gattaaatcg tcttatatgg aaaaatatat ggataaattt gttttattgc
      541 gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg ggcattgaaa
      601 caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa agaagtcaca
      661 gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt gttagtcgat
      721 ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt tgaagttcca
      781 gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct
      841 gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca agaacctcgt
      901 catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt
      961 agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct ggactgacga
     1021 tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat tagaaatctt
     1081 attatctaaa aaaggccgaa aaac
//
LOCUS       DAYLZO010000299         1099 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.316, whole genome shotgun sequence.
ACCESSION   DAYLZO010000299 DAYLZO010000000
VERSION     DAYLZO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.316"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            45..569
                     /locus_tag="ACGVJG_003225"
     CDS             45..569
                     /locus_tag="ACGVJG_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559100.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            597..1004
                     /locus_tag="ACGVJG_003226"
     CDS             597..1004
                     /locus_tag="ACGVJG_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559101.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            1004..>1099
                     /locus_tag="ACGVJG_003227"
     CDS             1004..>1099
                     /locus_tag="ACGVJG_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFJ6559102.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDE"
BASE COUNT          399 a          165 c          239 g          296 t
ORIGIN      
        1 gatggattaa ccaatattac atttgagaaa gtggggacgg tttaatggct gatattgtgc
       61 aattaaaaga agacggagtt gctaagtatt tgaaaactca tgccaaagga attgatggtg
      121 tggagggtgt tttagtaaaa gccacaggta atgaaacggt cttaggaacg aaaaatttta
      181 aagacggatt gcaattcaat ggattgcctg tgcaagcagg tatgatcgag cgagctatca
      241 cactagctga tcgaagtgat acaacaaacg taactgatgt gaatggaaaa ataatcagaa
      301 tcggaaacat cgtatttctt acttttaatt ttaaatgtgg cacatggccc gaaggatcag
      361 aaactcgttg gattttaaaa atcccggacg gtttcaaacg tgatcaaggc tatccagcgc
      421 aaaccgcgct ttcactagtt agaaatgcaa gtcagccagc ggatgcacgt gcattcattg
      481 atcaaagtag catcatacaa gccaaatcag gttcaggtag ttcatatatt tcaggtatgt
      541 ggatcactca agatccttgg ccggcttaac agattactaa ttggaggaag agtaaaatga
      601 aaacaattta taaagtattg tatccaatgg gatatgaaga acatcaagta gaagataact
      661 ttccaacatc tttaccattt gtcgaagttc cacctatttt gtttgaaaag aaggaagatg
      721 aaacggatga aggtttcgga agaagacaac agtcgcaatt ctttaacttc actgaaaata
      781 agtgggaaga agcagttaca caagattatt caaaaaaatt agaactacta gaaaatttat
      841 ctataggttt acaggtagat aatgctgcat tgaaaaaatc aaatgaagag ttaacggaaa
      901 aagcagattc gatggcgcag ttgaacgcca aactgatgct taatgattta acaattaata
      961 aaaaaattga agcaatagaa aaacaaattg gaggtgcttc ataatgttta gttttagcga
     1021 tgtgaaaatg atgtatgatt ggggctgttt tactgacgat caagttcgac aattcgttcc
     1081 actatgcatt acagacgaa
//
LOCUS       DAYLZO010000300         1090 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.317, whole genome shotgun sequence.
ACCESSION   DAYLZO010000300 DAYLZO010000000
VERSION     DAYLZO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.317"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            23..964
                     /locus_tag="ACGVJG_003228"
     CDS             23..964
                     /locus_tag="ACGVJG_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFJ6559103.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            966..>1090
                     /locus_tag="ACGVJG_003229"
     CDS             966..>1090
                     /locus_tag="ACGVJG_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFJ6559104.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEV"
BASE COUNT          419 a          171 c          247 g          253 t
ORIGIN      
        1 ccaattgatg aggaggatga agatgagtaa atctacctta gaaatgagcc atcaagaatg
       61 gctcgaagac cgcaaaagag gtattggtgg ctcagatgtt gcaaccgtac ttggattaaa
      121 caaatacaaa tctccttatc aattgtggct tgagaaaacg ggtcaaattg aattgaaaga
      181 tttagaaagc gaaccagctt attggggtaa tgttttagaa gaagtggttg ctaaagagtt
      241 tcaggaacgt acaggcaaaa aagtacgcag aagaaaccaa gtatttgaac atccattaca
      301 tccattttta agagcaaata ttgatcggga agtagtggga gaaaatgcca ttctggaatg
      361 taaaacagcc aatcaatttc tcggaaaaga gtgggaaggt gaagaggtac cgcttagcta
      421 tctctgtcaa gttcagcatt acatgaacgt tttaaacaaa gactattgtt atatcgctgt
      481 tttgatcggt ggacaaaaat tcatttggaa gcggattgaa cgagatcaag agctgatcga
      541 tacaatcact gaacaattag tagagttttg ggaaacgaac gttcttggag gtatcgagcc
      601 tgtaattgac ggaagtggag cgactgctga cttcttaaaa gaaaaatatg cagatgtaga
      661 agaaaatcaa acagctctac catcacgttt tgatgaactt atcgagcaaa aaagagaact
      721 caagcggacg aaaaaagaaa ttgaatcatc tattcgtcaa gtagacaacg agatcatcag
      781 tgagctaggt aaacgtgaag ccagtatcgg tatcacacaa agaaacatta tcagctggaa
      841 acttgttcgt acaagacgga tgaacacgaa gaaactagca gagaaatatc cagatgtcgc
      901 aaatgatgaa gagatttata acgttactga atcaagaagg ctaaccgaaa aggagatcaa
      961 ataatatggc aacaaatgaa tcgttaaaaa atcaattggc agaaaagcca cagaaacaag
     1021 ttgcaccagg acagttaggg cttaaagctc taatgaatac accaacaatg agaaagaaat
     1081 ttgaagaagt
//
LOCUS       DAYLZO010000301         1069 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.318, whole genome shotgun sequence.
ACCESSION   DAYLZO010000301 DAYLZO010000000
VERSION     DAYLZO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.318"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>1069
                     /locus_tag="ACGVJG_003230"
     CDS             <1..>1069
                     /locus_tag="ACGVJG_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFJ6559105.1"
                     /translation="RLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQNI
                     RRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSASG
                     QTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMDL
                     MNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITGP
                     NTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFSS
                     HMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYPE
                     LKVYGYNRANTINASMEFDVDT"
BASE COUNT          346 a          193 c          251 g          279 t
ORIGIN      
        1 acgtttgtat atgtgggcag atcagttagt cacgatccct gttttgagcc gccgtttaaa
       61 ggaagcaatc gatgaagacg gacgcgtgac tgatgacgct tctcctgagt taaagagcat
      121 aagacagaac attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg ggatcgtccg
      181 tgggaaaaat gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg accgctacgt
      241 tatcccagtc aaacaagaat accgcggtgt atttggcggt gtagtccacg atcaaagtgc
      301 ttcaggacag actttattta ttgaaccaaa acaagtggtg gatttgaata atcgtttgcg
      361 tcaataccaa atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac tttctgctga
      421 acttgttccc catcgtcaag aaatcatcca caatgcttat gtgattggta aaatggatct
      481 gatgaatgca aaagcacgat ttggtaaaga agtaaaagct atcgtgccgg gaatcagtga
      541 agacaatcat gtcgtcttga aacaagcaag acatccactg atcgatcagg aaaaagtagt
      601 ttctaatgac atcacaatcg gaaaagacta tcaagccatc gtcattacag gacctaacac
      661 aggtgggaaa acgatcacgt tgaagacttt aggtctgtta caactgatgg ggcaagcagg
      721 attaccgatt cctgcaggtg aagaaagtca aatcggtatt tttgaggaag tatttgcgga
      781 tataggagac gaacagtcga ttgagcaaag tctatcgacc ttttcttctc atatgacgaa
      841 cactgtagat attttatcaa aagtcaatga aaaaagtctg gtattatttg acgagctcgg
      901 tgcaggaaca gatccgcaag aaggtgctgc tctagcaatc gcaatcttag atgatcttgg
      961 taaaaagtct gcttatgtga tggcaacaac gcattatcct gaattgaaag tttatggata
     1021 taatcgggca aatacgatta atgccagcat ggagtttgat gtggataca
//
LOCUS       DAYLZO010000302         1061 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.319, whole genome shotgun sequence.
ACCESSION   DAYLZO010000302 DAYLZO010000000
VERSION     DAYLZO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.319"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(36..>1061)
                     /locus_tag="ACGVJG_003231"
     CDS             complement(36..>1061)
                     /locus_tag="ACGVJG_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFJ6559106.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DAYLZO010000303         1050 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.320, whole genome shotgun sequence.
ACCESSION   DAYLZO010000303 DAYLZO010000000
VERSION     DAYLZO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.320"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACGVJG_003232"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACGVJG_003232"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFJ6559107.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          359 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt
//
LOCUS       DAYLZO010000304         1046 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.321, whole genome shotgun sequence.
ACCESSION   DAYLZO010000304 DAYLZO010000000
VERSION     DAYLZO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.321"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..660
                     /locus_tag="ACGVJG_003233"
     CDS             <1..660
                     /locus_tag="ACGVJG_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFJ6559108.1"
                     /translation="MANGAYSAFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDA
                     PLKILLADADPEFVPNWLRKRNRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKW
                     LTNLNSVVMPMRYLWVFLAYMMLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFAC
                     ILGMVPKVDYTADPKAWWFQLFTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQ
                     P"
BASE COUNT          331 a          176 c          212 g          327 t
ORIGIN      
        1 atggcaaatg gggcatacag tgcttttcaa attttaggta attactatgg tatcggtaac
       61 tcattgatga tcatttacgc attgacaaac atgattggac aaatctccgc attagccttt
      121 tcaattgatg caccactaaa aatcttgtta gcagatgcgg atccagaatt tgtaccaaac
      181 tggctacgta aacgtaatcg taaaggtaca ttggtgaatg gttatcttct gacaggtata
      241 ctagtaggaa tcattatttt gttgccgatt tttggtatca aagatatgaa tgaattagta
      301 aaatggttga cgaacttgaa ctcagttgtc atgccaatgc gttacctatg ggtgttcttg
      361 gcatatatga tgttgaataa agcttataag aaattcaatt ctgaatataa atttgttaaa
      421 aatccaaaaa tcggttttat ctttggtgct tggtgctttg ccttcactgc gtttgcatgt
      481 atcctaggaa tggtgccaaa agtagattat actgctgatc ctaaagcatg gtggttccaa
      541 ttatttacga atatcatcac accaatcgtc ttgatcttat taggtatgat cttgccagca
      601 attgctcgtc gagacaaaaa caaagaaatc actgaaccag aaacagtggt tcagccttaa
      661 aatagaaata cttgttacaa gaggttgtga cagaagtggt cagcttcaag aaataagaag
      721 gaatttacga aaattgcttc tcaaattttt gtgaagtttc ggcttgtttt tcgaagaagc
      781 tacttctgga acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc aagtaataag
      841 aacagccaaa caaaaatagc ccctcatatt tttcgtttgg ctgggcttat taccgcagat
      901 gcaagctttg gaacaccgtt taaggaagag gttgtaaaat cagcgatttg acctgagtat
      961 tagagaaaca aacggaaaaa tagctcctca tattttgccg ctttttgagc taatgctgaa
     1021 ggtcagctga ttgaacacca tttatt
//
LOCUS       DAYLZO010000305         1035 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.322, whole genome shotgun sequence.
ACCESSION   DAYLZO010000305 DAYLZO010000000
VERSION     DAYLZO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.322"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..>1035)
                     /locus_tag="ACGVJG_003234"
     rRNA            complement(<1..>1035)
                     /locus_tag="ACGVJG_003234"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAYLZO010000306         1029 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.323, whole genome shotgun sequence.
ACCESSION   DAYLZO010000306 DAYLZO010000000
VERSION     DAYLZO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.323"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..464)
                     /locus_tag="ACGVJG_003235"
     CDS             complement(<1..464)
                     /locus_tag="ACGVJG_003235"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit EIIB"
                     /protein_id="HFJ6559109.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGV"
     gene            687..>1029
                     /locus_tag="ACGVJG_003236"
     CDS             687..>1029
                     /locus_tag="ACGVJG_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFJ6559110.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITG"
BASE COUNT          338 a          196 c          190 g          305 t
ORIGIN      
        1 acaccattcc aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata
       61 ccttcaagta catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt
      121 ggtgtaaaga tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt
      181 gcagctgctt ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg
      241 ttataaaaag tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac
      301 atccctttta cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc
      361 aggacaaaac gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg
      421 atatcatgta acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt
      481 ctcttaactt gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt
      541 gcaagcgttt tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa
      601 gcttttctta aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg
      661 atggaagaac gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg
      721 aacagaaaat tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat
      781 tgatcaaaat ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta
      841 cggaaggtta tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct
      901 tcgactttcc tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc
      961 caagtgaaac gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga
     1021 tcactggct
//
LOCUS       DAYLZO010000307         1022 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.324, whole genome shotgun sequence.
ACCESSION   DAYLZO010000307 DAYLZO010000000
VERSION     DAYLZO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.324"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..219
                     /locus_tag="ACGVJG_003237"
     CDS             <1..219
                     /locus_tag="ACGVJG_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFJ6559111.1"
                     /translation="QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEE
                     LGYLAIHIERLRSAIVQTNVNNHEEEKN"
     gene            216..>1022
                     /locus_tag="ACGVJG_003238"
     CDS             216..>1022
                     /locus_tag="ACGVJG_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFJ6559112.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENK"
BASE COUNT          281 a          200 c          228 g          313 t
ORIGIN      
        1 caacaatatg ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa
       61 agctataaga tttctaaaaa aatccgtgtg ttgctgacca aagaatacca gctcgcgatc
      121 acgaaagaag aactgggtta cttggccatc catatcgagc gtttaagaag cgcaatcgta
      181 caaacaaatg ttaacaatca tgaggaggaa aaaaattgaa agcatatcta caacgaatgg
      241 gacgttccct tcaacttcca gttgcggtct tacctgctgc tgctttacta gtagggatcg
      301 gaaactggtg ggcatcttat agtaacgaca ttgtcgctca ttttttacaa gctggcggta
      361 acgcagtttt aggtcaatta cctttgctgt ttgccgttgg gttagcacta gggatgtcag
      421 tggacaaatc aggtgctgct gccttggcag gtttggtcgc atttgaagtt ccagtaaatg
      481 ttttaaaaac tgattcagta gctactttat tgaatatcaa accagaatca gttgatccat
      541 cttttgctca gatcagtaac gtatttattg gtattattgc cggattgatt gcggcagctc
      601 tttataatcg tttccacgaa gcaaaattac cgatggcttt atctttcttt agcggcaagc
      661 gtctagtgcc aatcttggct gcctttgtca tgttgctgat cagtgcagtg ctactgctcg
      721 tatggcctcc ggtttactct gctttagttt cttttgctaa attcattttg agtttaggct
      781 ggggtggtgc aggtctttat ggcttcttca accgtttact gatccctaca ggattgcatc
      841 aagcaatgaa ctccgtgttc tggtttgacg tagcaggaat caatgatatt ggtaaatttt
      901 tggcttctga aggaacaaaa ggcgtaacag gaatgtatca agctggattt ttcccaatca
      961 tgatgttcgg gcttccagca ggagcatatg ctatttacag aaatgcacgt ccagaaaaca
     1021 aa
//
LOCUS       DAYLZO010000308         1003 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.325, whole genome shotgun sequence.
ACCESSION   DAYLZO010000308 DAYLZO010000000
VERSION     DAYLZO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.325"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..500)
                     /locus_tag="ACGVJG_003239"
     CDS             complement(<1..500)
                     /locus_tag="ACGVJG_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFJ6559113.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALA"
     gene            666..>1003
                     /locus_tag="ACGVJG_003240"
     CDS             666..>1003
                     /locus_tag="ACGVJG_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFJ6559114.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGM"
BASE COUNT          306 a          190 c          204 g          303 t
ORIGIN      
        1 gccaaagctt tgccgacatc ttttagttgt tcttcccatg tgacatctgt tccgtttttt
       61 acaacataag ttggcgcgat tttgctatat tgttcgtatt tcccgccttc gacagtagcg
      121 gaagaagaaa tcaacagcaa atcaggctca aagcttaaca ctttctcata aggaaggtca
      181 taagaaatcg ttgggacatc ctttaaatca tcttgcagat aatcttgaat ggaaccactg
      241 ccaacagtcc actgggcgat aggtttttcc cctagtgcaa tcagatagtc ttctaagtaa
      301 ctgccgatga tccgtttggg cgcgtttgga atctctactt tatgtccaag tgtatctgtg
      361 actgtatgag tactattgga tgaagtcgtc gaattggcag ttgtagctgt ttctttatct
      421 gaagaggggg agcaagcgct cagtactcca gccagcagca atatgctaga taatgtgaat
      481 acttttgttt ttaatttcat ttctttcctc ctaacataat tgagaatctt tctcattggt
      541 tattattcct gatatttcga acgctgtcaa ccgcttcatt tggctataga tatgaaagta
      601 gcgcttgacg aaatgttgaa acagcgtcat aattgagaat gattttcagc gaaaggatgg
      661 gtgaaatgac gagattaaga gcagctgaca ttgcagccgg ctacgaaaac aaaaaaatca
      721 ttgaaaacat gtctgttacg attcctgaag ggaaaatcac tagtttgatt gggccaaatg
      781 gaagtggaaa atctacttta ttgaaaagtt ttacccgtat actacctgtg aaatcaggaa
      841 agatcattgt caataactct gatatctcta catacagttc aaaagaactt gcaaaaaaaa
      901 ttgcattatt agcacaaact agtgaacacc ctacaggtat gacagtagaa gaagtagtat
      961 cttacggacg atacccttat caaaaactgt tcagtggcat gga
//
LOCUS       DAYLZO010000309         1003 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.326, whole genome shotgun sequence.
ACCESSION   DAYLZO010000309 DAYLZO010000000
VERSION     DAYLZO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.326"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>1003
                     /locus_tag="ACGVJG_003241"
     CDS             <1..>1003
                     /locus_tag="ACGVJG_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFJ6559115.1"
                     /translation="GKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTP
                     FCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCL
                     LFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIV
                     TDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYR
                     QLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDL
                     LTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLH
                     "
BASE COUNT          300 a          209 c          191 g          303 t
ORIGIN      
        1 tggcaagaag atctctatga ttctcctgaa tcgtagcggt aataaacgga cctatcttcg
       61 gttgaagaaa cagcgatacc tctgccgagc atgtaaaaaa tattttaccg ctcggacgta
      121 tctcgttacc ccattctgct ttatttctaa gcagattcat tacaagatct tagaagaatt
      181 gacagagcgt caatctataa aagcaattgg taaacattgc gatgtttctg tgacaactgt
      241 tcagcggaca ttgtccgcct tgtcatcaga tatccgtcag aatcttgagt atttacctcg
      301 gtgtctatta ttcgatgagt ttcgttctct ttctacatgg cacggtaaat tttctttttc
      361 ttgtatggac ggtgagaccg ggaaactgtt tgagatctta cctagccgcc gtaaaaaaga
      421 tctcgttgct tttttcatgc gtttttctct caaagcgagg ttgggtgtac attacatcgt
      481 aacggatatg aacgctcctt atttctcact agttaaagag tgttttccaa atgcacaagt
      541 gatcgtcgat cggtttcaca ttgttcaaca tctgaaccgc ttcttcgatg cggttcgaaa
      601 acgtgtgatg aaaacacttg atcaaaaaga cccaatacaa gctaaacagt atcgccaatt
      661 gaaatcctta ttcaaattac ttttaaaatc agaggatcgt ttagattaca atacactaac
      721 gaaaagacgc aatttcaatt ggagactatt gactgaaaca gaagtagttg accgccttct
      781 aaagatttct cctgaactta aaacagctta tcgttactac caagacctgc ttactgcgta
      841 tcaggaaaaa gatccagata cttttttcca gctgcttcga gcaatgcctg gagaagttcc
      901 attggaactg caccatatca aaaaagcctt tttcaaatac gagaaaggga ttcggctggc
      961 attactgaag aagtattcga atgcacgact ggaaaatctc cat
//
LOCUS       DAYLZO010000310         1000 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.327, whole genome shotgun sequence.
ACCESSION   DAYLZO010000310 DAYLZO010000000
VERSION     DAYLZO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.327"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            369..1000
                     /locus_tag="ACGVJG_003242"
                     /pseudo
     CDS             369..1000
                     /locus_tag="ACGVJG_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DAYLZO010000311          998 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.328, whole genome shotgun sequence.
ACCESSION   DAYLZO010000311 DAYLZO010000000
VERSION     DAYLZO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.328"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(80..634)
                     /locus_tag="ACGVJG_003243"
     CDS             complement(80..634)
                     /locus_tag="ACGVJG_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6559116.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          185 c          107 g          383 t
ORIGIN      
        1 ttccgaaaat tcagttatct tcattaatct caccttctaa gttttcatta tacacaaaaa
       61 atatatccta atatctcaat tagcctccca atttaaaacc acgtaacgat gcccctattt
      121 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
      181 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
      241 atgtatgtgt tcctatatca tctcttccta atttttctgc tacatctgca tatatctgat
      301 agacacgatc ccttgttata tgtcgacctt gttgccgact tggaaataac caatcatttt
      361 cttctttacc ttcagtatat aataaaatct cttctctcaa attatctata aaaatcggtt
      421 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttacctcgt atgtctccaa
      481 cttttaatcc tataatatct gacatacgta acccattatt gattccaaac aaaaataaaa
      541 aactatctct ttctggatta cttgttcttc ttagccaaaa ctgaaaatct tgtatctctt
      601 catttgtcct taacggttgt acattatagc tcactattgt ttcccttctt aaaaatattc
      661 tgagttcatg attatatata tattttacca taatcatgaa cttatatata tactctgtta
      721 aaagtgaaga aaatcaaggg gtttagttta tgattttata tcatttcagt aactcagaat
      781 attccgactt ttttgtacaa aaaaattaac cttacttata tgaagattct aagctcttat
      841 ttaaatttta gacgggcagt tatccgtaga agttcttaga atagctaatt tacataatta
      901 aattatatga aatttaagtt tgaacaatat actaagaact ccccttacta taaagtgaaa
      961 taaattagca tatgaataac cgtgctacca aacttttc
//
LOCUS       DAYLZO010000312          991 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.329, whole genome shotgun sequence.
ACCESSION   DAYLZO010000312 DAYLZO010000000
VERSION     DAYLZO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.329"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(42..115)
                     /locus_tag="ACGVJG_003244"
     tRNA            complement(42..115)
                     /locus_tag="ACGVJG_003244"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79..81),aa:Ile,seq:cat)
     gene            complement(670..984)
                     /locus_tag="ACGVJG_003245"
     CDS             complement(670..984)
                     /locus_tag="ACGVJG_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559117.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          369 a          155 c          154 g          313 t
ORIGIN      
        1 caaaaaattc taacacagaa cgattcaatt ggctacatta atgtaccctg taggactcga
       61 acctacgacc ggacggttat gagccgtctg ctctgaccaa ctgagctaag ggtagtggtt
      121 gttgccacat aaagccataa acaatcaacc agtagaatgc gtggcaacaa acctgttatc
      181 gcatatcttg gagtgtgact atttatgagt gatagtgaag atatgcgtct ccatatatgt
      241 atggttgaga cagataaaca tgtatgaaaa acgttgttaa atcaacgttt ttttattttg
      301 gtcaaggaga gaaaagtaga gaaatacaaa attttttgca ttgattttgc attcaaaaaa
      361 acattcaaac atttagaaat ccccaatacg tattcaggaa aatctatttt aaggtttaca
      421 aaaagaactt ttgttcgtac actctttttg aggtgaactt ttatgctgat ggaaggaaaa
      481 acacaattat ggtttaagca tgttccttca aataagtttt ttaattataa actctagaga
      541 aattaattca tcctaacata aaataattgt tcactcaaaa gtttttctag aacaattaaa
      601 acagcaggct tcacattcgc tatacatttg taagaagtaa taattcctgt actaaaaggc
      661 ataattgctt taattatttt ttgacttctc ttcaaacaca agaagtagtt gtgtaataat
      721 tgcagaaata aggattccaa taatagatat aaaatttgac atggaaacgt ctataacatt
      781 aaaagaataa agtgttgaag aaagtaaact gattgaaata acccctccgt aaaaaatagt
      841 ctgtgaatat acccactttt tttcagaaga cgtacttcgt ttggttctaa aacctatccc
      901 tgcatttcta ctcttatcaa aaaaccaaat atttagaagt aataatattg ctattattaa
      961 gataaaatta ctgtaaaaat ccaaaagcta t
//
LOCUS       DAYLZO010000313          980 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.330, whole genome shotgun sequence.
ACCESSION   DAYLZO010000313 DAYLZO010000000
VERSION     DAYLZO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.330"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..156
                     /locus_tag="ACGVJG_003246"
     CDS             <1..156
                     /locus_tag="ACGVJG_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFJ6559118.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..>980
                     /locus_tag="ACGVJG_003247"
     CDS             169..>980
                     /locus_tag="ACGVJG_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFJ6559119.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGT"
BASE COUNT          341 a          134 c          217 g          288 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaaa acactgaagt tatggaagct acaattgtcg aaaatggtct tccatatgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgttggcg gaaaatatcc agtccaatta
      361 gaaacaagaa ttgtggatgc taaaaaaggg aaaatcaaat atatttttga taaatattcc
      421 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      661 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      721 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      781 gccgaattaa tggatgcacg tgttgatatt caaggagttc gtcatgcgtc tatttcagag
      841 cgcttgctgg cagatatgga ttatctatat cagaaattac gagcaacact tttcacgatt
      901 gaatacggtg agattgaagt gactgatatt ttgcaggatg atctcttttc agataatcac
      961 gaagtcgaaa aagttggaac
//
LOCUS       DAYLZO010000314          936 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.331, whole genome shotgun sequence.
ACCESSION   DAYLZO010000314 DAYLZO010000000
VERSION     DAYLZO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.331"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>936
                     /locus_tag="ACGVJG_003248"
     CDS             <1..>936
                     /locus_tag="ACGVJG_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFJ6559120.1"
                     /translation="DVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGACPDCD
                     GLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDTPFEE
                     LPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFTREQM
                     RLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQEKVI
                     AKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVLYILD
                     EPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDED"
BASE COUNT          287 a          168 c          209 g          272 t
ORIGIN      
        1 gacgtgatcg gtgaagaaga aatgttgttt agcgaacact atgcctgtcc ttattgcgga
       61 tttactgttg gcgaactaga accaagactg ttctcattca atgcgccttt tggagcttgt
      121 ccagactgtg atggtttagg tgtcaaatta gaagtcgatc aagatttagt gattccagat
      181 agaacgaaga cacttagtca aggtgctatc gttccatgga atccaatcag ctctcagtat
      241 tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta tcgatttaga tacaccattc
      301 gaagaattac cggaagagca tcaggatatc atcttgaatg ggacagatga tcttttccat
      361 ttccattatg aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt cgaaggggta
      421 ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg tgagcaaatg
      481 cgtctttata tgacagaatt aacttgtcag acatgtaaag gttatcgatt gaatccccaa
      541 gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt gtctattcaa
      601 aaagcagtag atttcttctc aaacttaacg ttatctgaac aagagaaagt aattgccaaa
      661 ccgattttaa aagaagtaaa tgacagactt tcattcttgg aaaatgtggg actagactat
      721 ctaactttga gtcgcgcttc cggaacatta tctgggggag aagcgcagcg aatcagattg
      781 gctacacaaa ttggatcgaa tctttctgga gttttgtata ttttagatga accatcgatc
      841 ggattgcatc aacgcgacaa cgatcgactg attggctcat taaagaaaat gcgtgatctt
      901 ggcaacaccc tcgttgtagt cgaacatgat gaagat
//
LOCUS       DAYLZO010000315          885 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.333, whole genome shotgun sequence.
ACCESSION   DAYLZO010000315 DAYLZO010000000
VERSION     DAYLZO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.333"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..302)
                     /locus_tag="ACGVJG_003249"
     CDS             complement(<1..302)
                     /locus_tag="ACGVJG_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFJ6559121.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIP"
     gene            complement(354..716)
                     /locus_tag="ACGVJG_003250"
     CDS             complement(354..716)
                     /locus_tag="ACGVJG_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFJ6559122.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
BASE COUNT          249 a          210 c          138 g          288 t
ORIGIN      
        1 tctggaattt gcttagaaaa tccacgaaac aattgagaca aaataacgcc ggaatttccg
       61 cgcgcaccca tcagcaatcc ttttgaaagc attgctgcta attctcctac tttttctgag
      121 gttgaatcta ctactgcctt agctccacta gtcatagata gattcatatt tgttcccgta
      181 tcgccatctg gcacagggaa tacatttaat gaattgacgt attctgcatt tacttgcaga
      241 cgattcgcac ctgcctggac catttcctgg aactggcttg cacttatttc tgttacactc
      301 acctagataa tcctccttca gatcgactgc tgagttacca gccagtctcc tgcctagtct
      361 ggcagtacac gaacaccttg tacaaagaca ttaactgaat tggcagttac gcctaataat
      421 gcctctagat tgtatttcac tttttcttgg acgttgcggg aaacttcaga gattttcgta
      481 ccatagctga taatcgtata aacatctaca gcaataccat tatcttcttg gcggacaact
      541 actcctcttg agtaattttc tttacgcaaa atctcagtaa tattatcttt aatttgattt
      601 ttgctggcca ttcctacaac accgaacaca tctgtcgcgg cgccaccaac aactgttgca
      661 attacatcat tggtaatttc aatggttcca gctgacgttt gaatttttac agccatgaat
      721 gagcctcctt aacgggtaaa aaactcccat ttttcttcta tcatatttta tcatagcgca
      781 acttgaaata aaaggcagtt acccaaagta ctcaaaaacc ttgcaaaatt tcaggtataa
      841 ccttgtttaa tttacaatta ttcttctcaa ttgtcaattg tatct
//
LOCUS       DAYLZO010000316          867 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.334, whole genome shotgun sequence.
ACCESSION   DAYLZO010000316 DAYLZO010000000
VERSION     DAYLZO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.334"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            15..374
                     /locus_tag="ACGVJG_003251"
     CDS             15..374
                     /locus_tag="ACGVJG_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFJ6559123.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            389..550
                     /locus_tag="ACGVJG_003252"
     CDS             389..550
                     /locus_tag="ACGVJG_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFJ6559124.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            547..>867
                     /locus_tag="ACGVJG_003253"
     CDS             547..>867
                     /locus_tag="ACGVJG_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559125.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEE"
BASE COUNT          305 a          136 c          233 g          193 t
ORIGIN      
        1 aaaggtagta tcgcatgaat aaataccgta ataaaaaaac tgttcatcga ggtatcaagt
       61 ttgattctat cgcggaagca gagtattacg atctagcctt gtggcaagct gaagcgaatg
      121 gttggaaagt gaagctccag gaaaggtttg agctgatgcc gaagtttgaa ctagagggca
      181 agaagtatcg caagatcgag tatattcccg acttcacatt ttacaaaagc ggcaagctgg
      241 tcaaagtcgt agatgttaag gggatgcaga caaaagactt taagatcaag gcaaagttgt
      301 tctgtcatca atatcaagtg ccgttgatat tagccaaaaa atatcggaat acgttcaagg
      361 aagagcgttt ttaacgaggt ggtccatcat gacaacagaa gaagtgattc aaatgcgaat
      421 tcgaagcctt cggcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
      481 aacggctaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccgtagatt
      541 ggaggaatga gcgtggataa ggaagcggct tggcgaaaat taatgttgct gattcaagat
      601 gagaactggc aagaagatga agcagtggtt gctgaagttc agcgtctagg aaagattgct
      661 aacggacgta tacgaaaaaa gccagacaaa agaaaacagc gcaaagggaa aatcgtcgtt
      721 gttttacacg aaggcaaaat tttgatgcaa ggaacagcta gtgagctgtc tgcagaaact
      781 ggatatacgc gtgggactat tcgaacgtac gcttggcgaa atcacaccga tcgaaaaggg
      841 cacgaatata agtatttgga ggaagaa
//
LOCUS       DAYLZO010000317          856 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.335, whole genome shotgun sequence.
ACCESSION   DAYLZO010000317 DAYLZO010000000
VERSION     DAYLZO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.335"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..434)
                     /locus_tag="ACGVJG_003254"
     CDS             complement(<1..434)
                     /locus_tag="ACGVJG_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6559126.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACGVJG_003255"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACGVJG_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFJ6559127.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          258 a          139 c          136 g          323 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatg
//
LOCUS       DAYLZO010000318          849 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.336, whole genome shotgun sequence.
ACCESSION   DAYLZO010000318 DAYLZO010000000
VERSION     DAYLZO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.336"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..720
                     /locus_tag="ACGVJG_003256"
     CDS             <1..720
                     /locus_tag="ACGVJG_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFJ6559128.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          326 a          133 c          167 g          223 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
      841 atccatata
//
LOCUS       DAYLZO010000319          832 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.337, whole genome shotgun sequence.
ACCESSION   DAYLZO010000319 DAYLZO010000000
VERSION     DAYLZO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.337"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>832
                     /locus_tag="ACGVJG_003257"
     CDS             <1..>832
                     /locus_tag="ACGVJG_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFJ6559129.1"
                     /translation="IQDGVEFQNLGLVITDEQHRFGVNQRKVLREKGWRPDVLFMTAT
                     PIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLDSVLDWTKKELARGHQMYV
                     ICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKMKNQEKEVIMEAFKDNQMQ
                     ILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRVGRGSDASYCILVANPKNE
                     LGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLPQFAIADLVTDGNVLEVAR
                     "
BASE COUNT          258 a          145 c          211 g          218 t
ORIGIN      
        1 tgatccaaga tggagtcgaa tttcagaatt taggtctagt tattacggat gagcagcacc
       61 gttttggcgt gaaccagcgg aaagtacttc gtgaaaaagg ctggcgacca gatgtcttat
      121 ttatgacagc gactccgatt cctagaacat tagctattac agcatatggt gagatggatg
      181 tatctatcat tgatgaactt ccagctggac gtatcccgat cgaaacacgc tgggtaagaa
      241 cacctcaatt ggacagtgta ctggattgga cgaaaaaaga actggcaaga ggacatcaaa
      301 tgtatgtcat ctgtccgctg attgaagaat cagaagcatt agatgtaaaa aatgcagtgg
      361 aaatctatga aaaacttcgt gaactatttg caccaaaata tgaagttggc ttgttacatg
      421 gtaaaatgaa gaatcaggaa aaagaagtaa tcatggaagc cttcaaagac aaccagatgc
      481 agatattagt ttcgactact gtaattgaag taggggtaaa cgtgccgaac gcgaccgtca
      541 tgctgatcat ggatgcggat cgatttggat tggcacagct gcatcaactt cgtggacgtg
      601 ttggacgcgg aagtgatgct tcctactgta tcttagttgc taatcctaaa aatgaactag
      661 gtgtggaacg gatgaaaatc atgacagaga ccaccaatgg ttttgtacta agcgaaaaag
      721 atctggaatt acgtggtcct ggtgaagtat ttggcttccg tcaatctggt ctgcctcaat
      781 ttgcgattgc tgacttggtg acggatggaa atgttttgga ggtagccaga ga
//
LOCUS       DAYLZO010000320          819 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.338, whole genome shotgun sequence.
ACCESSION   DAYLZO010000320 DAYLZO010000000
VERSION     DAYLZO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.338"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>819
                     /locus_tag="ACGVJG_003258"
     CDS             <1..>819
                     /locus_tag="ACGVJG_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559130.1"
                     /translation="PYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIK
                     PMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWSKTFTAADD
                     SHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRDSNNPKDYV
                     WDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKA
                     NANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGK"
BASE COUNT          303 a          150 c          159 g          207 t
ORIGIN      
        1 ccgtaccagc ctaatctact tgatgcaccg tattatttga gtaaggtggc tttgggtgag
       61 aatctaatta aaccagaatc acaacaacca gttactaata gtaactatct tattaacacc
      121 tataacatta aaccaatggt aaaaggtaag aagtatacca tcacacttga aggaactaag
      181 ccagcaacac aggtttttag accatttttc acacgagcaa caggagatgc atgggaggtt
      241 ggtgacttac aaccagtaga aggcttaact aatgtgtggt ctaaaacatt tacagcagca
      301 gatgactcac accctactac cccacaagtg cagatttatc aagtaccaag cacaagtgta
      361 ggacaatgta caattaagtg gttaaaacta gaggaaggca atactcgaac cccgaatatt
      421 agtgagtata aatatcgtgg tactggtatg cgtgattcaa acaatccaaa agattatgtt
      481 tgggatctag caccagaata tgtcgaagat aatttggcca cagatattaa aatttctgaa
      541 attactggta aagcaaacaa ttataccgat gggaaagtat cggagattaa ttcgcagttg
      601 actgcttcaa ttaatgaagt agacaccaca gctaaggatg ctcaaacaaa agcgaatgct
      661 aatgcgactg ctatagatga attagacaat aagatcgatg aacgcattaa tgatacagct
      721 actaccacat taacagttac aaacgggaat accggatcag caaagcttta tcgtgaagga
      781 aaaacagttt ctatatattt tgtggcttta aacggaaaa
//
LOCUS       DAYLZO010000321          818 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.339, whole genome shotgun sequence.
ACCESSION   DAYLZO010000321 DAYLZO010000000
VERSION     DAYLZO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.339"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..324
                     /locus_tag="ACGVJG_003259"
     CDS             <1..324
                     /locus_tag="ACGVJG_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFJ6559131.1"
                     /translation="TITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLDGE
                     KSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARLVH
                     ENRCK"
     gene            421..>818
                     /locus_tag="ACGVJG_003260"
     CDS             421..>818
                     /locus_tag="ACGVJG_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HFJ6559132.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGA"
BASE COUNT          279 a          127 c          185 g          227 t
ORIGIN      
        1 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
       61 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
      121 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
      181 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
      241 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
      301 gtgcatgaga atcgttgtaa ataaataaaa aaaggaggat aaattaacaa gaacgtatga
      361 aataagggta gtgacattca gttggctaaa ccttccacta ataaattgga gggaatagaa
      421 atgggaaaat accaagaatt agcgagaaaa atcgtagaaa atgtcggcgg gaaggaaaat
      481 atcaatggtt tgacacattg tatcacaagg cttcgtttca aattaaaaaa cgaagaaaaa
      541 gcaaatgatg agatcttgaa aaatatggac ggtgttgtga cggtaatgcg agcgggagga
      601 cagtaccaag ttgtcatcgg gaatcatgtg ccagtcgtgt ttgaagaggt tataaaagca
      661 ggaaacctta cttttgatga ggctgtttca acagaaaaaa tgagaccgtt tgatatgctt
      721 attgatggaa tatcgggctg tttccaacca ttccttgcta tcctagctgc tgacgggatg
      781 attcgtggac tgacagcatt ccttgtcttc ttaggcgc
//
LOCUS       DAYLZO010000322          816 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.340, whole genome shotgun sequence.
ACCESSION   DAYLZO010000322 DAYLZO010000000
VERSION     DAYLZO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.340"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..554)
                     /locus_tag="ACGVJG_003261"
     CDS             complement(<1..554)
                     /locus_tag="ACGVJG_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFJ6559133.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLG"
     gene            complement(578..>816)
                     /locus_tag="ACGVJG_003262"
     CDS             complement(578..>816)
                     /locus_tag="ACGVJG_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559134.1"
                     /translation="DPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNV
                     LVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
BASE COUNT          233 a          169 c          127 g          287 t
ORIGIN      
        1 cctaacttag aaatttcttc tttcagagca tctaacttgt cttgtagcgt tttagcttga
       61 ccagttaaat cagtaatctg ttgatttaag ctatccactc tacctttgat ttcagccata
      121 aaagcatcaa aagtttcgtt gtactttcga atcaactctt ctaattgcga aacatattca
      181 tcggcttggc cttgcgaaat gtcagacact cctagtgaga aaaaaatgat atcttgcgtt
      241 gttaaaattt gattgtcttt tctatattct acgtagcagt gtttataata tcctgcttca
      301 ctcataaatg tgccatcaag agaaaacgtg acttcttcac tagttacact agttgcaaca
      361 ttatctacgt aacggttaga tggtgttgtt ccttttaaag taaatgttcc gccactcgta
      421 tccatctgca agccatttag aaacggttta accgtcaccg taatcccttt atcaccttga
      481 cgagccataa tagctttggt gtagtttaat tctttgctga aatctaaagc caaattatat
      541 aaactgctag ccatttatat acctccttgt cttcgtttta aaaacgtttt tggtcaagca
      601 ctgtgctatc atatgctgta tcctctttta atctaatatc ttcataccct agacggtgtg
      661 ccactaaatt ccatctaact aatacgtttg gcttactagt ttcaatgatg aaatggtcaa
      721 tatcttcatg agtaacagca cacaaaacta gttctgtagg tgtcacatgt gtcatatacc
      781 gacttagatt tactgtttca gcaaacatgg ggtcaa
//
LOCUS       DAYLZO010000323          812 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.341, whole genome shotgun sequence.
ACCESSION   DAYLZO010000323 DAYLZO010000000
VERSION     DAYLZO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.341"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(1..609)
                     /locus_tag="ACGVJG_003263"
                     /pseudo
     CDS             complement(1..609)
                     /locus_tag="ACGVJG_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139242906.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
     gene            complement(735..>812)
                     /locus_tag="ACGVJG_003264"
     CDS             complement(735..>812)
                     /locus_tag="ACGVJG_003264"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFJ6559135.1"
                     /translation="KVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT          200 a          196 c          131 g          285 t
ORIGIN      
        1 atacgtacaa ggcgatgaac acctttttct gatttcagat aaccaaatgc gttgtgtcct
       61 ttgatcagta gggtcacact cttgattcct gcttcatcac ctgcttgata atctagtgtc
      121 tctacttgat acccatgctg ctctgcccag cgtgtataca tacgtaaaag catactgccc
      181 caatcttggg actccgttcc gccagcccct ggatgcaatt caacgatggc gttgtttcga
      241 tcataaggac catctaacaa catagataat tcatatgtct tcattttttc atctagcgca
      301 ttgatacgtg tttctgcttc ttcttgcatg gctgcatcag gttcttcctg caacatctcc
      361 accatcaatt cgatttcttc cagttcttca gcaagagaat ggaattgatc ataagtttct
      421 ttatttgcat tgttttcact gatcactttt tgagcagctt cgctatcatc ccaaaaacca
      481 ggttcagcca tttttgcttc tgcttccgca atgtcttctt ccagctggtc taagtcaaag
      541 agacctcctg aagcttgtga ccttttcctg catttctgtc aggagatttc gaatttcgga
      601 tatttccatg ttttttcctt ctttcactat acttttttca ttatatctag tctaacagaa
      661 tcagcaaata acagaaagaa ggtgagacga attcttaaaa aaagaatttg tctcaccaat
      721 cctgctgtca gaaattagtt tgcatttttt ccatgacagt tcttatattt tttcccgctt
      781 ccacaaggac aaggatcatt tcttccgacc tt
//
LOCUS       DAYLZO010000324          791 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.342, whole genome shotgun sequence.
ACCESSION   DAYLZO010000324 DAYLZO010000000
VERSION     DAYLZO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.342"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..672
                     /locus_tag="ACGVJG_003265"
     CDS             <1..672
                     /locus_tag="ACGVJG_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFJ6559136.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          292 a          123 c          147 g          229 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag c
//
LOCUS       DAYLZO010000325          791 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.343, whole genome shotgun sequence.
ACCESSION   DAYLZO010000325 DAYLZO010000000
VERSION     DAYLZO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.343"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..716
                     /locus_tag="ACGVJG_003266"
     CDS             <1..716
                     /locus_tag="ACGVJG_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFJ6559137.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DAYLZO010000326          789 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.344, whole genome shotgun sequence.
ACCESSION   DAYLZO010000326 DAYLZO010000000
VERSION     DAYLZO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.344"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>789
                     /locus_tag="ACGVJG_003267"
     CDS             <1..>789
                     /locus_tag="ACGVJG_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559138.1"
                     /translation="DGPYWLGKVPLGENIADHTVKFPIESGDHQIYQGYTEEELMIGQ
                     TYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVASSPKLFRV
                     YQYPQSTVGACRIDWLKIEKGDTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVED
                     NLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDEL
                     DNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGK"
BASE COUNT          275 a          149 c          163 g          202 t
ORIGIN      
        1 gatggccctt actggttagg taaagttcct ttaggcgaga atattgccga tcatacagtt
       61 aagttcccaa tcgaatctgg cgaccaccaa atatatcaag gttacacaga agaagagctt
      121 atgataggtc aaacgtatac tatcacgctt aaaggaacaa aacccgcaag tcaaaccttt
      181 gtagcgtata atcattggac tgctcgttta ggagaactaa agccagttga tgggttgaca
      241 gacgtatggt ctctaacatt cacaccaacg aatgttgtgg cgagttcacc taaacttttt
      301 cgtgtttatc agtatccaca atcaacagta ggcgcatgcc ggatcgactg gctcaagatt
      361 gaaaaaggcg acacacgaac cccgaatatt agtgagtata aatatcgtgg tactggtatg
      421 cgtgattcaa acaatccaaa agattatgtt tgggatctag caccagaata tgtcgaagat
      481 aacttggcca cagatattaa aatttctgaa attactggta aagcaaacaa ttataccgat
      541 gggaaagtat cggagattaa ttcgcagttg actgcttcaa ttaatgaagt agacaccaca
      601 gctaaggatg ctcaaacaaa agcgaatgct aatgcgactg ctatagatga attagacaat
      661 aagatcgatg aacgcattaa tgatacagct actaccacat taacagttac aaacgggaat
      721 accggatcag caaagcttta tcgtgaagga aaaacagttt ctatatattt tgtggcttta
      781 aacggaaaa
//
LOCUS       DAYLZO010000327          785 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.345, whole genome shotgun sequence.
ACCESSION   DAYLZO010000327 DAYLZO010000000
VERSION     DAYLZO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.345"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            270..>785
                     /locus_tag="ACGVJG_003268"
     CDS             270..>785
                     /locus_tag="ACGVJG_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFJ6559139.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT          273 a          122 c          169 g          221 t
ORIGIN      
        1 gatttagatg tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag
       61 tacatccgca actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag
      121 agttctatat tagagatgta aagagttttg gtgaagaggt ttgtgagtat ggcttttata
      181 accagggtgc aatgagaagc actaacagca gctagcttaa gaacgcatag gatatacttt
      241 ttatggaagt cacagaggag gaataaaatt tgaacaggat taacagagta acctctattc
      301 ttattcagct gcaatcaaaa aaaataattc ctgccaaaga aattgcacag cgatttaata
      361 taagcttaag gacagtttac agagatatcc ggacacttga agaagccgga ataccaattg
      421 gatctgaggc cggaaaagga tattttcttg tagagggctt cctacttccg ccggtaatgt
      481 ttacagcggc ggaagtgggt gcattgatta cagcagggaa atttttaaat tgccatggag
      541 atgaatcatt tataaaggat tttgattcag ctatgtataa aatcaaatct attttgaagc
      601 atggtgaaaa aaactatgca caggagttgg agaacagtat taacgtgtac agcacatctg
      661 gacagaaaaa tacattggcg gataacgtta ttgcagcaat tcagactgca atctgcaata
      721 aaagggtaat atcaattcaa tatcctgcat caggagggca agaaccggaa agcagaatga
      781 tagag
//
LOCUS       DAYLZO010000328          769 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.347, whole genome shotgun sequence.
ACCESSION   DAYLZO010000328 DAYLZO010000000
VERSION     DAYLZO010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.347"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..93
                     /locus_tag="ACGVJG_003269"
     CDS             <1..93
                     /locus_tag="ACGVJG_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HFJ6559140.1"
                     /translation="AGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            105..>769
                     /locus_tag="ACGVJG_003270"
     CDS             105..>769
                     /locus_tag="ACGVJG_003270"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFJ6559141.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAE"
BASE COUNT          257 a          180 c          166 g          166 t
ORIGIN      
        1 gctggtatcc gtaaaaaagg aacagctcct gcacgaccaa aagctacaaa cgataaaaca
       61 gccccatcac gcagaagccg ctcaagagtc taggaggaag aaaaatggca cgaacacaga
      121 aacaaaaagc agcacaaact catgcagtca gtttgatcac attaacaaac ccatcttcac
      181 caatcgccga acaatatcgt acgatccgga cgaatatcca gtttgcctcc gcagctggtc
      241 agcaaatcaa aacgattgtc gtgacttcat caggtcctgg agaagggaaa tccaccactg
      301 cagcaaacat tgcagtcgtt tttgctaaat caggtcaacg agttctacta gtcgatgcag
      361 atttacgtaa acccgtcgtc tataagacat tccagctgaa caatgccagt ggattaagta
      421 cagcacttag ttcatcagga agtgtagcag atgtgatcca acgaacgcca gtcgagaact
      481 tgtcgatttt gcctagtgga cctaagccac ccaacccatc agaattgtta agctcgccaa
      541 gaatggatca gatcctatcc gaagcaagac agttatttga cgtggtgata ttcgatatgc
      601 caccagtagt agccgtaaca gatgcacaga tcatgtcttt aaaaacggat ggcactttat
      661 tggtcgtacg tgaaaatatg tcacgtaaag aatccttgac taaagcaaaa gaactgctgg
      721 agatggtaca agcacgagtc ttaggtgttg tgtacaacgg agcagaaca
//
LOCUS       DAYLZO010000329          762 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.348, whole genome shotgun sequence.
ACCESSION   DAYLZO010000329 DAYLZO010000000
VERSION     DAYLZO010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.348"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..644)
                     /locus_tag="ACGVJG_003271"
     CDS             complement(<1..644)
                     /locus_tag="ACGVJG_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFJ6559142.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVA"
BASE COUNT          212 a          187 c          116 g          247 t
ORIGIN      
        1 gcaaccaact catgatgtgc cgcttgatat ttgatttgtt cttcattttc tccttcttca
       61 aaatacagcc ctgcgcccat gaatggtgca tgaaggatca tattgatctc attgaatgtc
      121 aaccaatatt tgacaagtcc tttatagcga ttgaagatga ctcggcacag attttcataa
      181 aaaccaacga gttctctgga acgccacgca ccatattctt tgatcaaatg catcggacaa
      241 tcaaaatgtg taatcgtcac aagcggctcg atcccgtact tttgacattc tttaaagaca
      301 tcctcataaa atttcaaacc agattcattt ggctcttttt catcgcccat tggaaaaata
      361 cgactccaag caatggacat ccgataagtc ttgaacccca tttctgcaaa taaagcaata
      421 tcttccttat aatgatgata catatcgatc gcaatttttg ctggataaaa atgattttca
      481 tcaaattcaa acattttctt ttttccagta atgacaggaa aacggtcttc tccgattggc
      541 acaacatcta cgttcgctaa cccacgtccg ccttcatcat aaccgccctc gcattgattg
      601 gctgctgtag ctccgcccca taaaaaatct tttctgaatc ccatcatact tcctccatta
      661 catgtgtatt gcttgataat tctcttgcca gttcatcttg gacttcgatt ccttctgctt
      721 ttaaccggct gattgtttcc ttgaattgcg gtaaatactg ct
//
LOCUS       DAYLZO010000330          743 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.350, whole genome shotgun sequence.
ACCESSION   DAYLZO010000330 DAYLZO010000000
VERSION     DAYLZO010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.350"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(153..>743)
                     /locus_tag="ACGVJG_003272"
     CDS             complement(153..>743)
                     /locus_tag="ACGVJG_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFJ6559143.1"
                     /translation="ERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQ
                     TAGNIFASVKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDE
                     NGYVEDDYRIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIY
                     VDRDNEGNGTLARSKKKSFNWYKKVIDSNGENLSND"
BASE COUNT          220 a          164 c          112 g          247 t
ORIGIN      
        1 tcatacatat gtagattctc ctttacttaa tcaagtaagc atactttaca gccaatgcga
       61 aaaaaagtct attgttctgc ttgcgtttct cgaatagctt tattatactc gaaaaatccg
      121 ctgaaaaaca atagactttt ataaatcatt gatcaatcat tcgaaagatt ttctccattt
      181 gaatcaatta cttttttata ccagttgaat gatttcttct tcgaacgggc taatgtgcca
      241 tttccttcgt tgtcacgatc gacataaatg aatccgtacc ttttcttcat ctcacctgta
      301 ccagctgata ctaaatcaat acatccccaa gtcgtatagc ctaatagatc gactccatct
      361 aattctactg catctttcat tgcctggata tgtgctgcca gatagtcgat tcgatagtca
      421 tcttccacat aaccattctc atcgggcgta tcgactgcac ctaagccgtt ttctacaata
      481 aatagtggtt tttgataacg atcataaata tcattcatcg ttattctcaa gcctaatgga
      541 tcaatttgcc aaccccattc acttgcttcc agatatggat ttttgacaga agcaaagata
      601 tttcctgcag tctgttccaa gagttcgttg tcagtagtcg aaactcgaga agaataataa
      661 gaaaaggaga taaaatcaac agtatgttct ttcagcaatg cggcatcccc aggttccatt
      721 ttgatctgaa tgccatttcg ttc
//
LOCUS       DAYLZO010000331          742 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.351, whole genome shotgun sequence.
ACCESSION   DAYLZO010000331 DAYLZO010000000
VERSION     DAYLZO010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.351"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            178..381
                     /locus_tag="ACGVJG_003273"
     CDS             178..381
                     /locus_tag="ACGVJG_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFJ6559144.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..731
                     /locus_tag="ACGVJG_003274"
     CDS             381..731
                     /locus_tag="ACGVJG_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFJ6559145.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          300 a           79 c          120 g          243 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata
      661 gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc
      721 ctcaacgata aaatatagta ta
//
LOCUS       DAYLZO010000332          731 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.352, whole genome shotgun sequence.
ACCESSION   DAYLZO010000332 DAYLZO010000000
VERSION     DAYLZO010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.352"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACGVJG_003275"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACGVJG_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFJ6559146.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="ACGVJG_003276"
     CDS             complement(481..675)
                     /locus_tag="ACGVJG_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559147.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DAYLZO010000333          729 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.353, whole genome shotgun sequence.
ACCESSION   DAYLZO010000333 DAYLZO010000000
VERSION     DAYLZO010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.353"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(333..446)
                     /locus_tag="ACGVJG_003277"
                     /pseudo
     CDS             complement(333..446)
                     /locus_tag="ACGVJG_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          266 a          115 c          112 g          236 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaa
//
LOCUS       DAYLZO010000334          728 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.354, whole genome shotgun sequence.
ACCESSION   DAYLZO010000334 DAYLZO010000000
VERSION     DAYLZO010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.354"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..209)
                     /locus_tag="ACGVJG_003278"
     CDS             complement(<1..209)
                     /locus_tag="ACGVJG_003278"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFJ6559148.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDE"
     gene            complement(306..536)
                     /locus_tag="ACGVJG_003279"
     CDS             complement(306..536)
                     /locus_tag="ACGVJG_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFJ6559149.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
BASE COUNT          272 a          138 c          100 g          218 t
ORIGIN      
        1 tgttcatcaa aatctacatc tacgtttaag tgacgaatca cttgattcat agtgaagaca
       61 tcagaaagaa tcggaacgtt gtctaatgtc gtaatacctt cttctgctaa caaactagca
      121 gctaaaatag gcaaaacagc atttttagct ccttcaattc ttactgttcc atttaattga
      181 ttgccacctc ttacgatgat ctcttccatg ttgttccctc caaaaaattc caatgagcat
      241 acacttactc aaaatctttt ctattataac atccttgctt ttaaaatagt accttttttt
      301 aaaatttaca ggctaacgaa aagatttcgg cataaagcga taacctctaa gaaaaaatta
      361 cttgcagtgt accctaaaaa aattgaaaaa agtacgattg ccattctaat ttgcgtatta
      421 tgcattggtc tgaaaaaagt ttcaatgcgt aacgaacgta aagaccagaa ggctaagtag
      481 ataaacgcca aatgactcac gattcgaagc accgcatcaa ttccgtatac ttgcataatg
      541 acgtcctttc taacgattgt tttataataa ctaataaaat gataatacca taaaatgtat
      601 aaaaggaaaa acaagtatac ttaaaaatat aggaatcccc ttacattttt taggaaataa
      661 atgctaaaaa tatgcaaaaa aagggactga gacaaaagtc ttgtcgcagt cccttattcc
      721 aaataaac
//
LOCUS       DAYLZO010000335          715 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.355, whole genome shotgun sequence.
ACCESSION   DAYLZO010000335 DAYLZO010000000
VERSION     DAYLZO010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.355"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>715
                     /locus_tag="ACGVJG_003280"
     CDS             <1..>715
                     /locus_tag="ACGVJG_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFJ6559150.1"
                     /translation="IYEVANGKYELELDYPLGTRFDEYFENDYQIKAKPNDQEEYHIF
                     FIDDKDIDTFLNTVTIYAQSRTNRLGRRVVTLAEVDSKTGQEAISIIETKMDKKSDIR
                     LYSDITAVSSTIFEARNVLNCIAGEQGSLLQYWGGEIKREPFKLSLLKRRGRNNVGTI
                     RYGKDMSGLKVKLDWTGIKTRLIPYADPQSEVGTTNRIYGSPVDSAYINNYPDVYTEH
                     VQFTEEQGVKDIKSLNKIAK"
BASE COUNT          265 a           93 c          143 g          214 t
ORIGIN      
        1 aatatatgaa gtagctaatg gaaaatatga attagagttg gattatcctt taggaactag
       61 atttgatgaa tattttgaaa atgactatca aataaaagca aagccaaacg atcaagaaga
      121 gtatcatatc ttttttattg atgataaaga tatagatact tttttaaata cagtaactat
      181 ttatgctcag agtcgtacaa atcgacttgg aagacgggta gtcactcttg cggaagtaga
      241 ctctaaaact ggtcaagaag cgatatcaat tattgaaacc aaaatggata aaaaatctga
      301 catacgactt tattctgata ttacggccgt ttctagcaca atctttgaag cgagaaatgt
      361 tttaaattgt attgctggtg aacaaggatc attgcttcaa tattggggtg gagaaattaa
      421 acgtgaacca tttaagcttt ctttgttaaa gcgaagaggt cgcaataatg ttggaacgat
      481 tcggtatgga aaagatatgt ctggtttaaa ggtcaaatta gattggacag gtataaaaac
      541 aagacttatt ccatatgctg atcctcaaag tgaggtaggt acgactaatc gaatttatgg
      601 ttcgccagta gatagtgcct atattaataa ctatcctgat gtgtatacag agcatgttca
      661 gtttacagaa gaacaaggag taaaagacat taagagctta aataaaatag ctaaa
//
LOCUS       DAYLZO010000336          690 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.356, whole genome shotgun sequence.
ACCESSION   DAYLZO010000336 DAYLZO010000000
VERSION     DAYLZO010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.356"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            59..205
                     /locus_tag="ACGVJG_003281"
                     /pseudo
     CDS             59..205
                     /locus_tag="ACGVJG_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            282..611
                     /locus_tag="ACGVJG_003282"
     CDS             282..611
                     /locus_tag="ACGVJG_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFJ6559151.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            601..>690
                     /locus_tag="ACGVJG_003283"
     CDS             601..>690
                     /locus_tag="ACGVJG_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFJ6559152.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNL"
BASE COUNT          269 a           82 c          126 g          213 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tattttagaa gattgaatag
      121 acaacaccgg gttgtttata aggtagatga agatgaacac gtggttgaaa tttattcagc
      181 aaggacgcat tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta
      241 tttactaagt aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa
      301 aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg
      361 aagttgaagc aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag
      421 aattttatga ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag
      481 atgagattct tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata
      541 ttgctgaaga tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa
      601 ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag
      661 aagccattga aagaaaaatt tttgaattta
//
LOCUS       DAYLZO010000337          677 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.357, whole genome shotgun sequence.
ACCESSION   DAYLZO010000337 DAYLZO010000000
VERSION     DAYLZO010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.357"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..134
                     /locus_tag="ACGVJG_003284"
     CDS             <1..134
                     /locus_tag="ACGVJG_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFJ6559153.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            460..573
                     /locus_tag="ACGVJG_003285"
     CDS             460..573
                     /locus_tag="ACGVJG_003285"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HFJ6559154.1"
                     /translation="MGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          283 a           74 c           88 g          232 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataaataata aagaattgaa cttctgagaa aacaaagaat
      181 cttttttctt tttttgaatt gactcatgga ttggcgttaa aattttattt tgaaaaagtt
      241 atttctggtt taactcaaaa aaagagatat gaatctaata gtgatagaaa ggatgttatt
      301 tttcatgaaa tggttgtttt agatttaata catataacta ataaatgcta ttgaatgtat
      361 ttaaactaat taacaaaaga atttgaattg ttaataaaat ccaataaaat atattaataa
      421 agtagataga aataaaaaaa caaagactaa acatatataa ttggattaaa cgcaatatct
      481 gcaaaatatt gtaaatttaa agcctttgta gaaaaattta ctgaaagaca aactgataag
      541 attattcagt atcatattac ctatactgat taaaattttt tgaaacggga tactaaataa
      601 aaaatttatt tataacagaa ctaattattt aatgcggatg cgccaatact tggtcctgca
      661 agagctattt attttga
//
LOCUS       DAYLZO010000338          670 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.358, whole genome shotgun sequence.
ACCESSION   DAYLZO010000338 DAYLZO010000000
VERSION     DAYLZO010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.358"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            4..>670
                     /locus_tag="ACGVJG_003286"
     CDS             4..>670
                     /locus_tag="ACGVJG_003286"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFJ6559155.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIE"
BASE COUNT          222 a          120 c          155 g          173 t
ORIGIN      
        1 aaaatgagta atcgaatgat tttaaatgaa acatcgtatt ttggaaaagg agcgattgag
       61 tcgatcgtcc ctgaatttca gcagcgagga ttcggcaaag cagctgtcat tactgacaaa
      121 ggattgatcg agcacggtat agcaacaaaa gtaacagaac ttctagataa agcagcaatc
      181 gcttatgctt tatacgacgg gatcgtacct aatccaacga tccaaaatgt aaaagatgga
      241 gtggcttttg ttaaagaggc agaggcagac tgtttgatcg caatcggtgg aggttcgcca
      301 atcgatacgg caaaagccat tgggattatt ttgacgaatc cagagttttc agacgtcatt
      361 agtttagaag gtgttgctga tacgaaaaat ccatgtttac ctatattagc tataccaacg
      421 acttctggaa cagcagcaga agtaacgatc aactacgtca tcacagatga agtaaatcac
      481 cgaaaatttg tttgtgttga tcctcatgat atcccaatcg tcgcgttcat cgacagcgat
      541 atgatgatgg gaatgccgaa aaaattggct gcatcaacag gtatggatgc tatgacgcat
      601 gctattgaag ggtttatcac aaaaggcgct tgggaaatga ctgatatgct gcatttaaaa
      661 gcaatcgaaa
//
LOCUS       DAYLZO010000339          670 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.359, whole genome shotgun sequence.
ACCESSION   DAYLZO010000339 DAYLZO010000000
VERSION     DAYLZO010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.359"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>670
                     /gene="guaA"
                     /locus_tag="ACGVJG_003287"
     CDS             <1..>670
                     /gene="guaA"
                     /locus_tag="ACGVJG_003287"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFJ6559156.1"
                     /translation="KLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTL
                     YTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDSIV
                     WRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGIRS
                     VGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDI
                     TSKPP"
BASE COUNT          193 a          147 c          151 g          179 t
ORIGIN      
        1 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
       61 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
      121 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
      181 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
      241 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
      301 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
      361 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
      421 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
      481 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
      541 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
      601 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
      661 ctaaaccacc
//
LOCUS       DAYLZO010000340          670 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.360, whole genome shotgun sequence.
ACCESSION   DAYLZO010000340 DAYLZO010000000
VERSION     DAYLZO010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.360"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>670
                     /locus_tag="ACGVJG_003288"
     CDS             <1..>670
                     /locus_tag="ACGVJG_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFJ6559157.1"
                     /translation="GFINKNKPFLVQVEKSYENSTISKLLELVENASSKKAPAENFIT
                     KFARYYTPVVVGLAVLLAVLPPLVVSGAAFSEWIYRALTFLVISCPCALVISVPLSFF
                     GGIGGASKIGVLVKGSNYLELLAQTETVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQY
                     VASAEQFSTHPIAQSVLEGYAGPLLPTANIQEFAGEGILAEVDGKQVLAGNHKLMERF
                     EISFP"
BASE COUNT          210 a          120 c          133 g          207 t
ORIGIN      
        1 ggatttatca ataaaaataa accattttta gttcaagtag aaaaatctta tgaaaattct
       61 actatcagca aattattaga attagtagaa aatgccagca gtaaaaaagc accagctgaa
      121 aatttcatta ctaaattcgc gcgctattac acaccagtcg ttgtaggatt ggccgtttta
      181 ttagctgttt tacctccact tgttgtttct ggagcggcgt tttctgaatg gatctatcga
      241 gcattgacat ttttagtgat ctcatgtcca tgtgcattag tgatctccgt accccttagt
      301 ttctttggcg gtatcggagg agcaagtaaa atcggtgtat tagtaaaagg ttcaaattac
      361 ttggagttgc ttgcacaaac tgagacagtg gtattcgata aaaccggcac attaacgaaa
      421 ggtgactttt cgatccagac gatcgataca aaaatcgatc caaagatatt catgcaatat
      481 gttgcgagtg cagaacagtt ttctacacat ccgattgctc aatctgtttt agaaggctac
      541 gcaggtccgc ttttaccaac agctaatatc caagaatttg caggtgaagg gatcttagct
      601 gaagttgatg gcaaacaagt attagcaggt aatcacaaat tgatggaaag atttgaaatc
      661 tcttttcctt
//
LOCUS       DAYLZO010000341          633 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.361, whole genome shotgun sequence.
ACCESSION   DAYLZO010000341 DAYLZO010000000
VERSION     DAYLZO010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.361"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>633
                     /locus_tag="ACGVJG_003289"
     rRNA            <1..>633
                     /locus_tag="ACGVJG_003289"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          189 a          125 c          189 g          130 t
ORIGIN      
        1 ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag gtgaaaagca
       61 ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt caaagcccgt
      121 taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg catgcgaggt
      181 taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt gagtatgtag
      241 tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg taaaacgcac
      301 tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt agcggagaaa
      361 ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc tagcctcgga
      421 attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg gttaccgaat
      481 tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga gtgataagat
      541 ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata tatgttaagt
      601 ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYLZO010000342          629 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.362, whole genome shotgun sequence.
ACCESSION   DAYLZO010000342 DAYLZO010000000
VERSION     DAYLZO010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.362"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..>629)
                     /locus_tag="ACGVJG_003290"
     CDS             complement(<1..>629)
                     /locus_tag="ACGVJG_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00374 family protein"
                     /protein_id="HFJ6559158.1"
                     /translation="LMILGMSQLSVRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTG
                     VKINRFLDNIPRLFFQKVAHIVLVSAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSF
                     NFLDQTVNLLVGFLLLGLARALSVKVKKAFLPTIALLSFGILNTITRTLSWQLILVYV
                     LILLAVWLSRKEFYRTKFVYSWGAIIFDASLFGFLFIVYAIAGYHSGRF"
BASE COUNT          243 a          122 c          109 g          155 t
ORIGIN      
        1 aaaaagcgtc cgctgtgata gcctgcaatt gcgtaaacga taaacaaaaa gccgaacaag
       61 ctagcatcaa agattatagc tccccaagaa taaacgaatt ttgttcgata aaattctttt
      121 ctggataacc acacagctaa cagaatcaag acatatacca atatcaactg ccaagataac
      181 gtacgtgtga ttgtatttaa aataccaaaa gatagtaaag caatcgtagg cagaaatgcc
      241 tttttgactt ttactgaaag tgctctagca aggccgagta ataaaaagcc aaccaataga
      301 ttcactgtct gatctaaaaa attgaatgag aacggcaata atacttgaaa tagccgactg
      361 attgcagaca gattagtgat tgttgataga agtaccatca tgattcccgc aaaatagaca
      421 gcacttacga gcacgatatg cgcaaccttt tggaaaaaca gtcgcggaat gttatctaaa
      481 aatcgattga ttttgactcc tgtatgtgtg atgaacaata gaagtcccgt cataaaaggt
      541 agaacataat aaaacaaccg ataatataat agccaaacta ctgctacatc ttgccgaaca
      601 gacaattgac tcattcctaa gatcatcaa
//
LOCUS       DAYLZO010000343          623 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.363, whole genome shotgun sequence.
ACCESSION   DAYLZO010000343 DAYLZO010000000
VERSION     DAYLZO010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.363"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..546
                     /locus_tag="ACGVJG_003291"
     CDS             <1..546
                     /locus_tag="ACGVJG_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559159.1"
                     /translation="EDIVKAIQEVNADKVIVLPNNKNIFMAADQAAEVADIPVAVVPS
                     RTISQGLTAMLSFNDQASLEENKETMTEMLSSVVSGQVTHAIRDTSIDGVEIKEGDFL
                     GMIDGKILISNPDITATALSTLEQMINEDTEIVTILAGDEGTVEQANEFAEKLTEKHP
                     DLEIEIHQGDQPVYPYLFSAE"
BASE COUNT          216 a          117 c          132 g          158 t
ORIGIN      
        1 gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt cttgccaaac
       61 aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat cccagtagca
      121 gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt caatgatcaa
      181 gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt tgtgagcggt
      241 caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa agaaggagac
      301 tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac agcaacagcg
      361 ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat cttggcaggt
      421 gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga aaaacatcct
      481 gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct attttctgct
      541 gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa aggccttcgg
      601 ttagtgatca ggtatgatcg ctg
//
LOCUS       DAYLZO010000344          621 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.364, whole genome shotgun sequence.
ACCESSION   DAYLZO010000344 DAYLZO010000000
VERSION     DAYLZO010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.364"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            65..310
                     /locus_tag="ACGVJG_003292"
     CDS             65..310
                     /locus_tag="ACGVJG_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559160.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            495..>621
                     /locus_tag="ACGVJG_003293"
     CDS             495..>621
                     /locus_tag="ACGVJG_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFJ6559161.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQ"
BASE COUNT          227 a           79 c          146 g          169 t
ORIGIN      
        1 gatcgtttca aatttgtaga gaagtataaa gaaatattag aagagtattt gaggaggaca
       61 gcaaatgata aaaaaactcg ttcaattcag catggattta tatgatatcg aatcaggagc
      121 aacactatct gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat
      181 tttgtgggta gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa
      241 agcggttgag tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga
      301 cagcgaatga taccgaagtt tagagcgtgg gatagagaaa aagacagaat gaaggcagta
      361 gctttggttg aatttgctga ggcgtataaa tcaggagaac tcatttcacg tacaaaataa
      421 aaaaagtcgg attcctccga ctatgaataa tatttccgac ataagtatta tatcataatt
      481 gggggaatca gaggatggta cttttcgatg taaagaaata tgagacacca agcgcgaaag
      541 atgttgatat ggaacgcaca aaacataatg tcgctgtgtt tctttcagca tatctatcag
      601 ctagatgtag agtagggcaa c
//
LOCUS       DAYLZO010000345          613 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.366, whole genome shotgun sequence.
ACCESSION   DAYLZO010000345 DAYLZO010000000
VERSION     DAYLZO010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.366"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..219)
                     /locus_tag="ACGVJG_003294"
     CDS             complement(<1..219)
                     /locus_tag="ACGVJG_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFJ6559162.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMM"
     gene            complement(330..>613)
                     /locus_tag="ACGVJG_003295"
     CDS             complement(330..>613)
                     /locus_tag="ACGVJG_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HFJ6559163.1"
                     /translation="TQHYLETGELIPEPTPAEKIATAKLHLQRLADLKGEKVACREFR
                     THAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL"
BASE COUNT          150 a          130 c          104 g          229 t
ORIGIN      
        1 catcatacga ccagctacac tagcggtcaa tcccatttct gctagttctt cttttgttct
       61 tggatcaaat agttcatgca attcttctga gttgtgcgtg cgttcaaatc gttttccaaa
      121 tggatcgatt ccatcttcac gcaattgttc cattttttga cgacgtacaa gcatttgatc
      181 atttaaatct tctacttgat gttgttgttc ctgtgtcaca aaagttcctc cattctcatg
      241 ttaatctctt tcctataatg ccaatgaaac acaaaaaaag caagccgaac ttggaatttc
      301 ggcttgaaaa ttcacgaaaa acttagaaat cacaacgttt gtttcaatag tactttttcc
      361 gctttttcta caaattgatc cagcaggtca ttgatttctt gctgtttttc tgtctgattg
      421 attgctactt tcactttcgc tgctcgagga atccctttta aataataagc cgcatgtgtt
      481 ctgaattctc tacaagctac tttttctcct ttgagatctg ctaagcgttg taaatgaagt
      541 ttagctgtag caattttttc ggctggtgtt ggttcaggaa tcagttcccc tgtttcgaga
      601 taatgctgtg tgc
//
LOCUS       DAYLZO010000346          602 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.367, whole genome shotgun sequence.
ACCESSION   DAYLZO010000346 DAYLZO010000000
VERSION     DAYLZO010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.367"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>602
                     /locus_tag="ACGVJG_003296"
     CDS             <1..>602
                     /locus_tag="ACGVJG_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559164.1"
                     /translation="TPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEP
                     SPAEMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLD
                     GIGDSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEE
                     VNEFAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGG"
BASE COUNT          194 a          105 c          149 g          154 t
ORIGIN      
        1 actccagact tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga
       61 ctgctgtttg tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa
      121 acatatgaac cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt
      181 gtccaaggtc aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta
      241 aaaatcggag aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat
      301 ttagatggta taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac
      361 gtgcatacag aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc
      421 aaaatcaaag tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa
      481 gtgaacgagt ttgcgaaaaa agaaacacca cgtatcccgt atggtatcgt agcgatcgct
      541 gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt
      601 gg
//
LOCUS       DAYLZO010000347          594 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.368, whole genome shotgun sequence.
ACCESSION   DAYLZO010000347 DAYLZO010000000
VERSION     DAYLZO010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.368"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>594
                     /locus_tag="ACGVJG_003297"
     CDS             <1..>594
                     /locus_tag="ACGVJG_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFJ6559165.1"
                     /translation="GSQTMDDFSPIYHVNKSIRQTTLVDLIKKGFQEYGTLIEENLPN
                     NLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQMRLQGLKKAEKAETN
                     GLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQRLLQGDVGSGKTVV
                     AAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDP"
BASE COUNT          199 a          116 c          127 g          152 t
ORIGIN      
        1 ggttctcaaa caatggatga cttttcgccg atatatcacg tcaacaaatc aattcgtcaa
       61 acgacattag ttgatctaat caaaaaaggg tttcaagaat acggcacatt gattgaagaa
      121 aatctgccaa acaaccttgt ggagaaatat cgactgcttg accgagcttc tgctgtaaga
      181 agtatgcatt ttccaaaaga ccacgaagaa aaccatcaag ccaaacgacg tgtcgttttt
      241 gaagagttct ttttatttca aatgcgtctt caaggattga aaaaagcaga aaaagcagaa
      301 acgaatggtt tggagatcct ttatgatgtc caaaaattga aagaatttac tcaaaagcta
      361 ccttttgaac tgacaaatgc acaaaaacgt gtaacgaatg aaatctgtcg ggatcttcgc
      421 agtccgcagc atatgcagcg acttttgcag ggagatgtag gtagcggaaa aacagtggtt
      481 gccgcgattg cgttatacgc taccgttaca gcaggtttcc aaggtgcgtt gatggtgcct
      541 acagaaatct tggcacaaca acatatggaa agcttgcaac agctgtttga tccc
//
LOCUS       DAYLZO010000348          584 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.369, whole genome shotgun sequence.
ACCESSION   DAYLZO010000348 DAYLZO010000000
VERSION     DAYLZO010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.369"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>584
                     /locus_tag="ACGVJG_003298"
     CDS             <1..>584
                     /locus_tag="ACGVJG_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFJ6559166.1"
                     /translation="SINEGDVVCIIGPSGSGKSTFLRCLNRLEEPSDGEIIIDGAHLM
                     DKSTDINLVRQHIGMVFQHFNLFPHLSILENIILAPMDLKKESREEAEKKAIKLLQTV
                     GLEDKKDMYPEMLSGGQKQRVAIARALAMNPDIMLFDEPTSALDPEMVGDVLNVMKKL
                     AEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFLE"
BASE COUNT          183 a          111 c          131 g          159 t
ORIGIN      
        1 tccatcaacg aaggagatgt cgtctgtatc attggccctt caggttcagg aaaaagtaca
       61 ttcttgcgtt gcttgaatcg tttagaagaa ccttctgacg gtgaaattat tattgacggc
      121 gcccacttga tggataaaag cacagatatc aacttggtga gacaacatat cggaatggta
      181 ttccaacact ttaacctatt ccctcatctt tctattttgg aaaacatcat tttagcaccg
      241 atggacctga agaaagaatc tagagaagaa gcagagaaaa aagcaatcaa attactgcag
      301 acagttggtc tagaagataa aaaagatatg tatcctgaaa tgctttctgg tggacaaaaa
      361 cagcgtgtgg ctattgctcg tgctctagcg atgaaccctg acatcatgct ttttgatgag
      421 cctacttctg cactcgatcc tgaaatggtc ggtgatgtct tgaacgtcat gaaaaagtta
      481 gcagaacaag gtatgacgat ggttatcgtt acgcacgaaa tggggttcgc taaagaagtg
      541 gccaatcgcg tcatgttcat tgacgacgga aactttttag aaga
//
LOCUS       DAYLZO010000349          583 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.370, whole genome shotgun sequence.
ACCESSION   DAYLZO010000349 DAYLZO010000000
VERSION     DAYLZO010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.370"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            87..>583
                     /locus_tag="ACGVJG_003299"
     CDS             87..>583
                     /locus_tag="ACGVJG_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFJ6559167.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          221 a          106 c           94 g          162 t
ORIGIN      
        1 taaatgaaaa aatccataca aaaaaggaag tcccttctgt agaataaagt taacgacaac
       61 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
      121 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      181 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      241 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      301 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      361 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      421 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      481 aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag
      541 ccttaaatga taagtatgtc gctattttta tggacgctac tta
//
LOCUS       DAYLZO010000350          574 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.371, whole genome shotgun sequence.
ACCESSION   DAYLZO010000350 DAYLZO010000000
VERSION     DAYLZO010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.371"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..570
                     /locus_tag="ACGVJG_003300"
     CDS             <1..570
                     /locus_tag="ACGVJG_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFJ6559168.1"
                     /translation="RLVYNGNEFTPIATISEAIKNQTIIINGVSKTYAMTGWRIGYAV
                     GNPVIINGMIAVASQSTSNPTAVSQYAAVEALKGEQDTVEEMRNAFEERLNTLYSLVA
                     ELPGVSLEKPQGAFYLFPNVKETLRMCKYENVTQWVEDLLEETGVALVTGEGFGAPEN
                     VRMSYATDRATLEEAVRRIKQFIESKSQN"
BASE COUNT          181 a          107 c          138 g          148 t
ORIGIN      
        1 cgtttagttt ataatggcaa tgagtttact ccaattgcaa cgatttcaga agcaatcaaa
       61 aatcagacta tcatcatcaa cggcgtgtct aaaacatatg cgatgacagg ctggcgcatt
      121 ggttatgcgg tgggaaatcc tgtgatcatc aatggaatga tcgctgttgc ttcccagtca
      181 accagtaatc cgacagcagt cagtcaatat gcagctgttg aagcattgaa aggcgagcaa
      241 gatacagtgg aagaaatgcg gaacgccttt gaagaacgcc tgaatacgtt atattcgctt
      301 gtggcagaac tgccgggtgt ttctcttgaa aagccacaag gagcttttta tctttttcct
      361 aatgtaaaag aaacgctacg catgtgtaaa tatgaaaatg tcacacaatg ggtagaagat
      421 cttttagaag aaacaggggt agcgcttgtg actggcgaag gctttggggc accagaaaat
      481 gtccgcatga gttatgctac tgatcgcgct acgttagaag aagcggtacg cagaatcaag
      541 caatttattg agagtaaaag tcaaaattaa tcct
//
LOCUS       DAYLZO010000351          573 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.372, whole genome shotgun sequence.
ACCESSION   DAYLZO010000351 DAYLZO010000000
VERSION     DAYLZO010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.372"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            65..>573
                     /locus_tag="ACGVJG_003301"
     CDS             65..>573
                     /locus_tag="ACGVJG_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559169.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          199 a           72 c          130 g          172 t
ORIGIN      
        1 ttattgcaac tgttgatgat ccaaaacaga atgttttcac tcttgagaaa gttggggtga
       61 tttgatggtt aaagtaaaga gaatgatgga gactgatgaa tctggtgtta aacgtcaatt
      121 tatgccaatt acacatgttt ctgcagtttt aggcttagaa aaaatgatat caggacaatc
      181 aaaagttctt tcggttaatg gtaaatatgg tgcagtcatt ttaacgaaag ctgatttagg
      241 attagagaat gcaattactg aactacctta tgcaagcgag acaagcgatg gtattcttac
      301 tgctgaaatg tttcaaaaga tcgtaaatgg agaaggtgga acatatattt tgccgattgc
      361 tactcctgaa caattaggag gtataaaagt tggggaactc ttagaagtta ctgaagaagg
      421 tgttttatct gcaactaagc aaacagactt caatttctct gaagaattaa agggaaaatt
      481 agaatctcta aaaatattaa aagcaggtgc aaatatatca attgcagaag atggaacaat
      541 tagttctaca ggtggtagtg ggactggtgg tgt
//
LOCUS       DAYLZO010000352          570 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.373, whole genome shotgun sequence.
ACCESSION   DAYLZO010000352 DAYLZO010000000
VERSION     DAYLZO010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.373"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>570
                     /locus_tag="ACGVJG_003302"
     CDS             <1..>570
                     /locus_tag="ACGVJG_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFJ6559170.1"
                     /translation="KLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNGGIKDEAL
                     TQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTKAAKMYKE
                     FGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFAKSVESKG
                     FEVEDVTLGVPDVDKETQKSIDAIIRAGQ"
BASE COUNT          201 a           87 c          131 g          151 t
ORIGIN      
        1 caaattgggt gtagcaggag cagtaatcgt aggtattgga gttatcggag gatttaagtt
       61 cttcgaaaaa atcgataatg gatatgtggg tgtgcgctat tcaatgaacg gcggtatcaa
      121 agatgaagca ctgacgcaag gtgtgaaatt tgtagggatt gacaaagtga tccaatatcc
      181 aattcgcttg caaactatcc aatcaaaaaa tatttcagta tctacaagcg acggcaaaaa
      241 gacaacgatt gatatcaaat atgactacaa agttgattca actaaagcag caaaaatgta
      301 caaggaattt gggaatatca cttcggaaga tatcgaaagt ggatggttaa aatctaagct
      361 tcaaaaggtc gctcgtgaag tttatgcgaa atatagtctg cttgatgtcc tttcaggaga
      421 ttcctctaaa gttgaagctg aggtattaac gaactttgct aaatcagttg aatctaaagg
      481 gtttgaagtc gaagacgtaa cacttggtgt tccagatgtc gataaagaaa cacaaaaatc
      541 aatcgatgcg atcattcgag ctggtcaaga
//
LOCUS       DAYLZO010000353          569 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.374, whole genome shotgun sequence.
ACCESSION   DAYLZO010000353 DAYLZO010000000
VERSION     DAYLZO010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.374"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
BASE COUNT          185 a           81 c           81 g          222 t
ORIGIN      
        1 tcttgatgtg atatccttgt cttttttgta atgtttttaa cttattgctt ttgcaaaatt
       61 tatttacctg ttttctggat caacgatagt gtacattatg ctatccttgt ttggatttta
      121 ggacgtttaa agaggcactt ttttacataa cttaatccat agacctttta agcatgctgt
      181 gagataataa taaagaagag tttaaagcgc accccaaacc acttccccat aagtgtatta
      241 cgctttaaac tcttttatat ttgaattcac taagaagcat accatatttt tgaaaaaaag
      301 tgagaaaaaa ggcttataat taggatttag agtaattaat tagatgttta ttttatagga
      361 ctgatactat agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg
      421 tgaataagat aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac
      481 ctttctggat caacgaaagt atactttata tagtcataac gccgatggta actatgcact
      541 gtaacgataa tgctgctaaa atatctttt
//
LOCUS       DAYLZO010000354          567 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.375, whole genome shotgun sequence.
ACCESSION   DAYLZO010000354 DAYLZO010000000
VERSION     DAYLZO010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.375"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>567
                     /locus_tag="ACGVJG_003303"
     CDS             <1..>567
                     /locus_tag="ACGVJG_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFJ6559171.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIREWTC"
BASE COUNT          210 a           89 c          102 g          166 t
ORIGIN      
        1 tatatttctt tgttatgcaa agaagaagtt cgcgaactac ctaaatctaa ttctgctgtt
       61 ggtattgacc taggtattat agactttgca attctttcta ccggtcaaaa atttgataat
      121 aataaattta cgtccaaaat ggaaaagaaa ctcaaacgtg aacaacgtaa gttgtcaaga
      181 cgtgccttat tagctaaaca aaaaggtatc aatctgtttg aagctagaaa ttatcaaaca
      241 caaaaacgta aagtagcacg attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat tatcaaaaat cacgatatca tctgtattga agacttaaac
      361 acaaaaggca tgttgcgtaa tcataaacta gcaaaaagta tttcggatgt ttcttggtct
      421 agttttgtgt ctaaattaca atacaaggct gactggtacg gtcggaaaat catcaaagta
      481 gataagtggt ttccatctag ccaaatctgt tcgaattgtg gacataaaga cggcaagaaa
      541 tctctcgaaa ttcgagaatg gacttgc
//
LOCUS       DAYLZO010000355          564 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.376, whole genome shotgun sequence.
ACCESSION   DAYLZO010000355 DAYLZO010000000
VERSION     DAYLZO010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.376"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>564
                     /locus_tag="ACGVJG_003304"
     CDS             <1..>564
                     /locus_tag="ACGVJG_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFJ6559172.1"
                     /translation="HGVPIANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSAD
                     KGFLDDPRFSAYGVLAPKSKADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIR
                     KALLENGAIDTVIGLPANIFFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNT
                     LRDYHIDAILEAYKTRTIKDKYAYVAE"
BASE COUNT          169 a          125 c          121 g          149 t
ORIGIN      
        1 tgcatggggt tcccattgcc aaccaacact tgcataatgc ggataccttg gatcaagact
       61 ggccaacaga agagccaacg aacttcgatg gggtgctcat gaacccaccg tattctgcaa
      121 aatggtcggc tgataaaggc ttcttagatg acccacgctt ctctgcctat ggcgtattag
      181 cccctaaatc aaaagcggac tttgctttct tattacatgg ctactatcat ttgaaagata
      241 cgggcgtcat ggccattgtt ttgccacatg gggtgctctt tagagggggc gcagaaggga
      301 agatacgcaa agccttgtta gaaaatggtg ccattgacac cgtcattggc ttacctgcta
      361 acatcttctt taatacgtcc attccaacga ccgtaattat cttgaagaaa gaccgtgcga
      421 aaaaagatgt gctctttatt gatgcgtcac aagattttga aaaaataaaa acgcaaaata
      481 cgctacgaga ttaccatatt gacgctattt tagaggccta taaaacccgt acaattaaag
      541 acaagtatgc ttatgtcgca gaat
//
LOCUS       DAYLZO010000356          548 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.377, whole genome shotgun sequence.
ACCESSION   DAYLZO010000356 DAYLZO010000000
VERSION     DAYLZO010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.377"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..392)
                     /locus_tag="ACGVJG_003305"
     CDS             complement(<1..392)
                     /locus_tag="ACGVJG_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFJ6559173.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQ"
BASE COUNT          163 a          123 c           76 g          186 t
ORIGIN      
        1 aactgtcctt tataaaagac cgtataacgg tatttcccat tcgatttttt acgtctatat
       61 gcaaacgcac catcttgcgc aatatcttct ccagaacgca aggcaccacc tttaacaatt
      121 ttccctccag taattgtaga taaaacttta tgagtatatc catttgcttc cagttcaact
      181 tctgcaccac caaaagctgt aaattgatct tgaaccactg aatccccata atcttgtgtt
      241 gtttcataat tagctgttgg tttgatactt actgctgttc ccattggcaa cgcagtacca
      301 tataccggaa aattccctgt ctcatcttcc aaaggaaacc aagtaggttt ttcgacagaa
      361 ataattccgg ttttacttct tttttctccc atttatcttt cactccattc aatatattgt
      421 ggaaattcta atgtaaaacg aatatgttga acaccatctg cttcatcagg taaataggct
      481 tttggaaata atatttgctc atcgattgta atcgtattaa aaaaagtccc acaacgtgag
      541 actaattc
//
LOCUS       DAYLZO010000357          547 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.378, whole genome shotgun sequence.
ACCESSION   DAYLZO010000357 DAYLZO010000000
VERSION     DAYLZO010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.378"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..149
                     /locus_tag="ACGVJG_003306"
     CDS             <1..149
                     /locus_tag="ACGVJG_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFJ6559174.1"
                     /translation="NGKLVKVVDVKGMQTKDFKIKAKLFCHQYQAPLILAKKYRNTFK
                     EERF"
     gene            164..325
                     /locus_tag="ACGVJG_003307"
     CDS             164..325
                     /locus_tag="ACGVJG_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFJ6559175.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            322..>547
                     /locus_tag="ACGVJG_003308"
     CDS             322..>547
                     /locus_tag="ACGVJG_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559176.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLS"
BASE COUNT          213 a           85 c          132 g          117 t
ORIGIN      
        1 aaaacggcaa acttgtcaaa gtcgtagatg tcaaaggaat gcagacaaaa gactttaaga
       61 tcaaggcaaa gttgttctgt catcaatatc aagcgccgtt gatattagct aaaaaatatc
      121 ggaatacgtt caaggaagag cgtttttaac gaggtggtcc atcatgacaa cagaagaagt
      181 gattcaaatg cgtattcgaa acattcagcg tgaaattgac gatctagaac gaacaaaggc
      241 agtgatggtc aatgaaacgg cgagaaaggc aatcgatttg cacatagaga acttaagaag
      301 ggaaattcgt agattggagg aatgagcgtg gataagaaag cgacaatgaa acgaattgct
      361 gaattaacca agtcagaatc ttggcaggaa gacaaagaaa tagttgcaga agtccaaaag
      421 ctcggtaaat caatgtggac tgaaaagcct aaacggaaaa cgccgagaaa aattgcaatc
      481 tggcatggtg atcgaattct agtaacaggt acagctgaac agttatctga aattactgga
      541 ttaagca
//
LOCUS       DAYLZO010000358          536 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.379, whole genome shotgun sequence.
ACCESSION   DAYLZO010000358 DAYLZO010000000
VERSION     DAYLZO010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.379"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
BASE COUNT          185 a           79 c          113 g          159 t
ORIGIN      
        1 acctttgaaa aagaacatcc aacgcttttc agtgaacaat gatttcttgc ttgaacaccg
       61 tgaattagag gtcgtgaaaa agctgtcctg tatcaagtaa taagaacagc caaacaaaaa
      121 tagctcttca tattttgtcg ttttttgagc taataccgag ggtcagttga ttgaacaccg
      181 tttatatcag aggttgtgag cctgccgttt aactctgagt cacaaggagc aatttcaaaa
      241 aacagcttct catgtttgat gaatattttg acttgtgacc gaagagttgg taggagaaca
      301 ccgtttataa gaggttgtga caaaagtctt gtcgcaacct cttttttaac caaaaaagtg
      361 caaatagttt actttaagta agagaataat tatgatgaat aaaaaaggtg gaacaaatag
      421 catactatat atattgaaat gaacactgta tggatgctcc aacttgatga agctaaggag
      481 gaattcgtat gtcagaaaag gaattaatga aggtagtcgg tttttatgaa gggttg
//
LOCUS       DAYLZO010000359          533 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.380, whole genome shotgun sequence.
ACCESSION   DAYLZO010000359 DAYLZO010000000
VERSION     DAYLZO010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.380"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..>533
                     /locus_tag="ACGVJG_003309"
     CDS             <1..>533
                     /locus_tag="ACGVJG_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFJ6559177.1"
                     /translation="TRDRAGYEVRDVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVN
                     KFGFIETPYRRVDRETGRVTDQIDYLTADIEDHYIVAQANSPLNEDGTFAQDVVMARA
                     QSENLEVSIDKVDYMDVSPKQVVAVATACIPFLENDDSNRALMGANMQRQAVPLINPQ
                     APWVGTGMEYKSAHDSGA"
BASE COUNT          155 a          122 c          121 g          135 t
ORIGIN      
        1 actcgtgacc gcgccggtta tgaagttcgt gacgttcact actcccacta tggccgtatg
       61 tgtccgatcg aaactcctga aggcccaaac atcgggttga tcaatagttt gtccagttat
      121 gcgaaagtaa ataaattcgg atttatcgaa acaccttatc gtcgtgtaga ccgtgaaaca
      181 ggtcgtgtta ctgaccaaat cgactacttg acagctgata tcgaagacca ttacatcgta
      241 gcccaagcaa acagcccatt gaatgaagat ggaacatttg ctcaagacgt tgttatggct
      301 cgtgctcaaa gtgaaaactt agaagtatct attgataaag tagactacat ggacgtatca
      361 cctaaacaag tagttgccgt tgcgacagca tgtattcctt tcttggaaaa cgatgactcc
      421 aaccgtgcct tgatgggtgc caacatgcag cgtcaagcag tgccgttgat caatcctcaa
      481 gcaccatggg ttggtactgg tatggaatac aaatcagcgc atgactcagg agc
//
LOCUS       DAYLZO010000360          532 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.381, whole genome shotgun sequence.
ACCESSION   DAYLZO010000360 DAYLZO010000000
VERSION     DAYLZO010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.381"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(<1..203)
                     /locus_tag="ACGVJG_003310"
     CDS             complement(<1..203)
                     /locus_tag="ACGVJG_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6559178.1"
                     /translation="MCLTAGVTTGAFYKHFTSKEALFEEIIQPHINALINMYNEEFTK
                     YLLNLENIDIEKSWRENFNELDP"
BASE COUNT          179 a           94 c           71 g          188 t
ORIGIN      
        1 aatggatcta attcattgaa gttctctctc caacttttct ctatatctat attttctaag
       61 tttaagaggt atttggtaaa ctcttcatta tacatattaa taagcgcatt aatatgcggc
      121 tgaataattt cttcaaataa cgcttcctta gaagtaaaat gtttataaaa agcacccgta
      181 gttacgcctg cggtcaagca cagttgcctt atcttagttt gctgaaatcc ttctttttca
      241 aaatggctta gtccactttc tattaatttt tggtgagttg taatataatc tctggtttct
      301 atgggtactt cctcctaata agatgaatta actatattat cttacacagg acttgaagtt
      361 tcaatcgtta acgtctatta ctgaactcat tccttagagc taaactaatg agaaagagca
      421 aaataactga tacaaaaagc aatcctacaa gaattaatag actcatctaa atcattcacc
      481 ttttctaaaa taatccgaga aagatttaaa aaaattatat ccagctatta gg
//
LOCUS       DAYLZO010000361          528 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.382, whole genome shotgun sequence.
ACCESSION   DAYLZO010000361 DAYLZO010000000
VERSION     DAYLZO010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.382"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(5..90)
                     /locus_tag="ACGVJG_003311"
     tRNA            complement(5..90)
                     /locus_tag="ACGVJG_003311"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="ACGVJG_003312"
     tRNA            complement(107..178)
                     /locus_tag="ACGVJG_003312"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="ACGVJG_003313"
     tRNA            complement(190..262)
                     /locus_tag="ACGVJG_003313"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="ACGVJG_003314"
     tRNA            complement(280..361)
                     /locus_tag="ACGVJG_003314"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="ACGVJG_003315"
     tRNA            complement(369..441)
                     /locus_tag="ACGVJG_003315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="ACGVJG_003316"
     tRNA            complement(445..517)
                     /locus_tag="ACGVJG_003316"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAYLZO010000362          521 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.denovo.383, whole genome shotgun sequence.
ACCESSION   DAYLZO010000362 DAYLZO010000000
VERSION     DAYLZO010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.denovo.383"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            146..493
                     /gene="rbfA"
                     /locus_tag="ACGVJG_003317"
     CDS             146..493
                     /gene="rbfA"
                     /locus_tag="ACGVJG_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFJ6559179.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT          192 a           86 c          113 g          130 t
ORIGIN      
        1 atgacgtgaa agaagtcaaa atgggttatg aatgtggtgc aatgattgaa aaattcaatg
       61 acatcaaagt agatgacgta attgaaggat ttatcatgga agaagtcaaa aatgactaaa
      121 aactgataaa aggaggccaa tcattatggc taactaccgt gaccgccgag tcggtcaaga
      181 aatcttaaaa gaagtcaatg atattttgcg caaaaaggtc cgtgaccctc gtgtggagaa
      241 cgtaacgatc acagatgtga aagttacggg ggacttacag caagcaacga tctattacag
      301 cattttatct gacttagcat cagataagaa aacagcacaa gatggattga ataaagcatc
      361 aggattgatt cgtcgagagt tagggcacaa tctaagtatt tataagacac ctgaattgtt
      421 ctttgaactt gatgaatcag ttgtctatgg caaccatatc gatgaattgc ttcgcgactt
      481 gaacaaagac taatcaaaaa ctcccgcaaa tcctgtgaaa t
//
LOCUS       DAYLZO010000363        22633 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.037, whole genome shotgun sequence.
ACCESSION   DAYLZO010000363 DAYLZO010000000
VERSION     DAYLZO010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.037"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            196..2385
                     /locus_tag="ACGVJG_003318"
     CDS             196..2385
                     /locus_tag="ACGVJG_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFJ6559180.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2382..4175
                     /locus_tag="ACGVJG_003319"
     CDS             2382..4175
                     /locus_tag="ACGVJG_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFJ6559181.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            4375..4689
                     /locus_tag="ACGVJG_003320"
     CDS             4375..4689
                     /locus_tag="ACGVJG_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFJ6559182.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            4710..5087
                     /locus_tag="ACGVJG_003321"
     CDS             4710..5087
                     /locus_tag="ACGVJG_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFJ6559183.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            5097..5870
                     /locus_tag="ACGVJG_003322"
     CDS             5097..5870
                     /locus_tag="ACGVJG_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559184.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            5892..7295
                     /locus_tag="ACGVJG_003323"
     CDS             5892..7295
                     /locus_tag="ACGVJG_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFJ6559185.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            7477..8661
                     /gene="mobT"
                     /locus_tag="ACGVJG_003324"
     CDS             7477..8661
                     /gene="mobT"
                     /locus_tag="ACGVJG_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFJ6559186.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            8658..8948
                     /locus_tag="ACGVJG_003325"
     CDS             8658..8948
                     /locus_tag="ACGVJG_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559187.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            8945..9166
                     /locus_tag="ACGVJG_003326"
     CDS             8945..9166
                     /locus_tag="ACGVJG_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559188.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            9208..9987
                     /locus_tag="ACGVJG_003327"
     CDS             9208..9987
                     /locus_tag="ACGVJG_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFJ6559189.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            10048..10548
                     /locus_tag="ACGVJG_003328"
     CDS             10048..10548
                     /locus_tag="ACGVJG_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFJ6559190.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            10604..11242
                     /locus_tag="ACGVJG_003329"
     CDS             10604..11242
                     /locus_tag="ACGVJG_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFJ6559191.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            11314..11709
                     /locus_tag="ACGVJG_003330"
     CDS             11314..11709
                     /locus_tag="ACGVJG_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFJ6559192.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            11693..14146
                     /locus_tag="ACGVJG_003331"
     CDS             11693..14146
                     /locus_tag="ACGVJG_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFJ6559193.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            14143..16170
                     /locus_tag="ACGVJG_003332"
     CDS             14143..16170
                     /locus_tag="ACGVJG_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFJ6559194.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            16167..17189
                     /locus_tag="ACGVJG_003333"
     CDS             16167..17189
                     /locus_tag="ACGVJG_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFJ6559195.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            17206..18135
                     /locus_tag="ACGVJG_003334"
     CDS             17206..18135
                     /locus_tag="ACGVJG_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFJ6559196.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            18449..18496
                     /locus_tag="ACGVJG_003335"
                     /pseudo
     CDS             18449..18496
                     /locus_tag="ACGVJG_003335"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18512..20431
                     /gene="tet(M)"
                     /locus_tag="ACGVJG_003336"
     CDS             18512..20431
                     /gene="tet(M)"
                     /locus_tag="ACGVJG_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFJ6559197.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            20529..20717
                     /locus_tag="ACGVJG_003337"
     CDS             20529..20717
                     /locus_tag="ACGVJG_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFJ6559198.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20775..21128)
                     /locus_tag="ACGVJG_003338"
     CDS             complement(20775..21128)
                     /locus_tag="ACGVJG_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6559199.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21659..22081
                     /locus_tag="ACGVJG_003339"
     CDS             21659..22081
                     /locus_tag="ACGVJG_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFJ6559200.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            22078..22308
                     /locus_tag="ACGVJG_003340"
     CDS             22078..22308
                     /locus_tag="ACGVJG_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6559201.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
BASE COUNT         7814 a         3645 c         4325 g         6849 t
ORIGIN      
        1 aaggtttcca acaggccgaa ggcgagctcg aacaaatgct aagccaactg attgagaatt
       61 agaaacaatc tgcagtaggg aagaatcatt tacacaaaat tattgacgct cccttataat
      121 gagaactttt actaacataa tttatgataa acttactata taatcatatt aattaaatag
      181 ttaggagatc tgcttatgtt tatatctact cttcaaattc gaaattttag aaattatgaa
      241 tccgaaatct ttcgattcga taatgagaca aatactatta taggtgagaa tgattcaggg
      301 aaaaccaatg ctcttactgc attgagaatc ttattagatg atagttatta ctatagtagt
      361 aaaacactaa aagaatcaga tttttttcat ggaattcaaa atgggtggca agggcattgg
      421 attattatct ctgcgacatt tgaaggaatc agcgaagaag aatttgacaa tgagatttgt
      481 gcttccattt ctttaaattc tgaaagtcaa acaatcttag aagaattgat ttcaaatgca
      541 gataaaggtg taggtagtat ttctctattt attagaccta acaaagctat tagaaagcaa
      601 ttatttgata tttccactga attagactct catcaattta acgaatttag aaattctatt
      661 cgtctatccg actatgaatt ttactacaca tctaaatcaa atctaaattt ctgtatcaat
      721 gaaaattatg ataaattggt aggaagactc aatgacaata tagctagtaa tcctgaagaa
      781 gacgatgaag ctctgttggg ctcaagaatt gacatgtcag atattttcaa gcatatttcg
      841 gttgtttatg ttgacgctct tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca
      901 gtcaaaagaa tcatggaaac aattgaatca aaaatatcat cagataatgt tgagtcatta
      961 aaaacaatta tacagcaatt aaatgagact attaccagtg ttcctgaaat taggaaaatt
     1021 ggcgaaaata ttaatcggca actaaatagc ataattggtt cagtgtattc tcctaatctt
     1081 cttctttcct caactatgtc agatgatatg ggttctttag caaaatttct ttcaatgaag
     1141 ccagaacaaa atatcgactt agacttacta gggttaggac atctaaatat gatttattta
     1201 gctttgaaaa tagtcgaatt tgaagcgtgt cgttcaagag aactacttaa tattatgttg
     1261 attgaagaac cagaagctca tattcatcat catattcaaa aaactctgtt cgaaggatta
     1321 aatcttcaaa aaaactatac tcagattctc atgaccacac attctgtaca tttagctgaa
     1381 gtgtctgaaa tatctcggat gaatgtatta aaatcgttta atggcaaatc tatcgttatg
     1441 aatccagcaa ataatctgaa ctcatttgct aaagaaaagt taaaaaagaa caatctaaat
     1501 ctaataaacg cagtagagag atatttagat gttaaaagaa atggattgct tttttctaaa
     1561 ggtgttatct tagttgaagg agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa
     1621 ttatttggta taaacttaga cgaacttgga ataggtttga ttaatgttgg tagtacttcg
     1681 tttgaatata tcgctagctt gtttgacgat gatagaatac agagaaagtg tgctattgta
     1741 actgatttag atgaacaggc aatcccatca gaacatcgat tgtataaaag cactgcacaa
     1801 gagaaaggtc aagcaagaaa agaaaaatta tcctcccttt ttaatgaaaa caattgggtt
     1861 aattgttttt atgctaatac aacatttgaa attgagtttt tagatgtgaa taaagaaaat
     1921 attctaactt atgtcagtcc aattatagat gacgcattca aagataaaaa aacaataact
     1981 gcttataaag aagaaataca aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa
     2041 ctagccaatc atgttggtaa ggggtggttt gcaattgagt taggtgaaaa aattgacttt
     2101 ttaatcaaaa ttcctaagta tattcttaat gctattgctt ttgcatgtca ggaaatagta
     2161 gatctctcaa tttataaaaa aattctactc catacaatcg acttgtacga ggaaactaca
     2221 gaattgtcag ctttatcagc tagtttaaaa acaagtgata cagctgattt gaatatagca
     2281 ataatttctt tattagagta ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag
     2341 atagaaaatt acacaaaaat actttatgct gaggtttcaa aatgacaaat atcttcgaaa
     2401 aaatcgtaaa tgatttaaat ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt
     2461 atgtaaatgc atgtcctggg agtgggaaaa caagggtact tacaaggaaa attgcttacc
     2521 aagcaattaa acacgatgga tcactaaaaa gaataattgc tatcacatac actaatagag
     2581 ctgctgaaga aattaaggaa cgcctaagtt tacttagtat agatgatgat attaatattt
     2641 gggtggggac aatacatcag ttttgtcttg aatttattat ttatcctttt aaaatgaatc
     2701 tctcaagagt atcaaaagga tttacaatta ttgatgatta tactcaaaga gcttacgtca
     2761 gccaaattaa taatatgctg aatttaaatc tcaaaacgta tgaactgaac aaaattgatt
     2821 tgtcactttc tcatgatttt aaaattaatg aagttcagta tactcaagtt tctgaaatgt
     2881 atcacactat gttggaaaaa aataaagaaa ttgattttga tatgatttta ctagtagcgt
     2941 ataaaatttt aactgtaaac agtaacgttg cacgtaatat tgctagtaat ataagggcga
     3001 tttatgtgga tgaatttcaa gatactagag aattacaata caatgtaata gctaaattac
     3061 ttcaaaacaa ccctcaaatt cagtcaatgt ttgttggaga tattgaccaa gcaatttatg
     3121 gatcattaga tggtatagct aaatcagttg aagaactaga aatgctaact agtcacactt
     3181 ttcaatcaaa gacgctacat ggctgttaca ggtctaatca gagattagta gacttctact
     3241 ctaattttca gagttgtcct tacgagattg aaagcagagg gaataataaa aacgaccgtg
     3301 gttttataag ttatgattgc aaagttacta aggaggattt acccaacata ataaaaaata
     3361 taataaagga aaagattgaa agcggcattc atgaaaaaga tatttgtgta cttgctcctc
     3421 aacactatcc tctgttttca ctgggtgagt atttaaagaa agaattaccg tattgcaatt
     3481 ttgatgctat aaatatatca ccaattaagg taaataatca tagtttattt ttcaagttag
     3541 caattttgta ctttactgaa tcaggtgaaa aggttcgaag aagaaagcta attgcaaacg
     3601 atattttaat tatattaaaa aatgaatttg gaatagaagt taaagattat tttattgact
     3661 tagatttatt acagctaata aacagtgtga aaagaatgta tagtggagac gacaacggtg
     3721 ttgatttata caaatttgtt accaaacata tatttgttaa tcttaatatt gacgagaaaa
     3781 attacccgtc tatttttaag cactatctat tttttattaa cgaagtagaa gatcggataa
     3841 aaaataattc attatctagt agtgtatcta gttttaagaa gatgtttaaa tctcgagatg
     3901 gtatcacact tactacagca cataaagtta aaggaaatga gtatgatact gttatcgcta
     3961 ttaatcttct aaacgggaaa attcctcatt ggaatgaaat ttttaatgtt tctgataaag
     4021 gaattagttc agcgagaaaa ctactatacg taatttgttc aagagcaaaa aataatttat
     4081 ttcttttctc agaggtgggt tattttacga gtaaaggaga cttgctttat cccactcaat
     4141 tattagataa aatagaattt gattataata tttaaaatac ctaggtagtc gttgttttaa
     4201 atatagtagg aatcggacgc tcagtacgga gcgtctattt ttttacccaa ttttgaaagg
     4261 aagtgaactt atgaaaataa aaaatcaaga atcaaaaggt cgctctcccc cctttaaaac
     4321 catcacacaa ataagattca ttcaaacata aaaacagaaa ggataggtaa aaatatggaa
     4381 cttaaatatg tcattcccaa tatggaaaaa acatttggac aattagaata tgctggtgaa
     4441 gggaacattg aacaacgtcg aataaatggc agaccaacca ttctctctcg tagttacaat
     4501 ttatactcaa cgattcaacg agcagatgat attgttgtca ttcttccctc cgaagctggc
     4561 gagaaacatt ttgaatccga cgaaaaagtc aaactcatca acccacatat taccgcagaa
     4621 ggttataaaa ttggcaatcg aggatttacc aattacatta tgcacgcaga cgatatggtg
     4681 aaagcataag aaaggaaagg tgaaaaaata tgagattagc agaaggtatt gtgattgaaa
     4741 aagaccagac atttggcaca ttgaaatttt ccgccttgcg tcgtgaggtt cgtatccaga
     4801 atgaagatgg tacggtttcc gaagaaatta aagaacgtac ctatgactta aaatcaaaag
     4861 gacaaggacg catgatacaa gtcagtatcc ccgcttctgt tcctttgaaa gaatttgact
     4921 acaacgcaga agtggaatta atcaatcctg tggcagatac cgtagcgacg gctacttttc
     4981 gaggggcaga cgttgattgg tacgtgaaag cagacgatct tgtattaaaa ggacagacaa
     5041 aacctcatca gaaatataaa aaagaagcgt ccgaaagtga aaaataggtg ttgcttatga
     5101 caaacacacc attgattttg aaagaaattc cgaaggatga agccatttct tttattcggc
     5161 aataccatta ttcaaagata ctccctagat tgtgtaaata ctttctaggg attttttcag
     5221 aagaaaaatt attaggcgtt gtggaattag gctggggtac gcaaccccta caaaccatac
     5281 gcaaactctt tcccgacagt tcccttcaaa ccacagatta tttagaaatt ggaaagatgt
     5341 gttttcttcc agaaatgaat caaaccaatt attttggcag tcaagctctt tcagctctaa
     5401 ttaagtggtt gaaagaacat acggactgtc attttttata tacactggca gatggaattg
     5461 agggaaaatg tggctatgtc tatcaagcgt ccaacttctt ttactgcggg tatttcaaga
     5521 caagtgtcta tcgtgataag cagtcatggg aaaagataca ccctcgaagt gctcgccttt
     5581 tattagagga aaatgctcgc tttgaacagg tagagaaaaa gcattggctt tcccaggcat
     5641 tttgcgaata caaagggatt gagaaaatca atggacgcat gtttcgctat ctctatccgt
     5701 tgacaaaaga agcgaaaaaa ctgttaggac acactctcta tcgacgtcat tattatccga
     5761 aagaaaaaaa tttacgcttt gaaaagcgaa tcgcttatca gaaatatgaa gccatttctc
     5821 aaccaacttt tgataaacag gctcgtatct acaatacaca attattttga taggtggaaa
     5881 ggaggatttt tatgccattg cttcatgaca gaggagagcg aatcaaagcc aacgataagc
     5941 acttggtcta tcattttgta ctaatttcat tgctggtagt ttttctagtc gtcctactcc
     6001 cttttcattg gagagcgtta caatcggtga actggcaagc agttcagtta gatacatttt
     6061 tctatcaact tcatacgcca tacttttgga gcagtattct cactgctctt tttgtatgcg
     6121 gacttatcag ctggctttat tatcactatc gtatagattc tataaaacag ttagagcacc
     6181 gtcaaaagtt agctcgtatg attttagaaa atcattggta tgaagccgaa gaaactcaat
     6241 cagagggatt ctttaaagat ttacccagca agaaaaccaa acaacgaatc cgccattttc
     6301 ctaaaatata ttatcgaatg aaagatggac taatccatat tcatgtagaa atcaccttag
     6361 ggaaatatca agatcagctt ttgcacctag aaaacaaact agaaacagga ttgtattgtg
     6421 aattagtgga aaaaacgttg aaagattctt atgtagagta tgtcctactt tatgatacta
     6481 ttggcaatcg aattggcatt gaagacgtgg tagctcaaga tggacgtgtc aaattgatgg
     6541 aatcggtcta ttgggagttc gattctctcc ctcacatgtt aattgctggg ggcactggta
     6601 gtggaaaatc ctattttatt ctcacattga ttgaatctct cctgcataca aatgcaaagc
     6661 tttatattct tgaccctaaa aatgctgatt tggcagactt aggggcggtc atgaataatg
     6721 tctattaccg taaagaagat atgttggcat gtatcaatca attttacgaa gatatgattg
     6781 atcgcagtga aaccatgaaa caacacccta actataaaac aggagaaaat tatgcttatc
     6841 ttggtttgtc tgccaatttt cttatttttg atgaatatgt ggcaatgatg gatatgctag
     6901 gtcgtgaaag taccaccgtt attcataaac tcaaacaaat tgtaatgtta ggacgacaag
     6961 caggcttctt tttaattctt gcctgtcaac gtccagacgc aaaatatttt agtgatggga
     7021 ttcgggacaa ctttaacttc cgtgtggctt tgggtcgtat gtctgaaatg ggctttggta
     7081 tgatgttcgg aagcgataca caaaaacaat ttttcttaaa acccatcaag ggaagaggtt
     7141 acgtggatac aggaaaaagt gtgattagtg agttttatac accccttgta ccaaaaaggt
     7201 acgatttctt aggagaaata ggaaaagtca ttcaaaaaaa gcaatctgag cctgtaccgc
     7261 acgaagtgaa aggtacaggt tcagacttgc tttgattggt gtggcgttag ccacgccaat
     7321 cctttagccc cctctatcta acaggggggc acaaatacaa ctgaaaacag gcaattctca
     7381 tcaaccaatg ttgatggtac aaaggattgc ggaaaatatg aaaggtgtaa actcaagggt
     7441 catagtttac atctttttta gattggaggt ttgcgaatgg aaaacgaaaa aatttggatt
     7501 aaggaattga aagaaaagcg actggcttat ggcgtttctc aaaataaact ggcggttgct
     7561 tctcatatta ccagacctta cctatcggat attgaaacag gcaaggcagt ccctactcaa
     7621 caggtgaaag aagatttact gaacgctttg gaacgcttca atcccgataa cccattggag
     7681 atgttattcg actatgtacg gattcgattc cctaccaatg atgtggctca aattatcggt
     7741 gaggttttac gtttgaatat ggattatatg ctacacgaag actttggcta ttattcttat
     7801 cccgaacact atcgtttcgg tgatattgtt gtcctagtct ctcatgatgt aagtaaaggt
     7861 gttttattag aactgaaagg aaaaggctgt cggcaatttg aaaactttct attggctcaa
     7921 catcggagct ggtatgattt ctttcaagat tgcatggaac ataaaggtat tttcaaacgc
     7981 ttggatttag ctatcaacga taaaacaggg attttgaata ttccagattt ggcaaagaaa
     8041 tgtaaacagg aagaatgcat ttccgtcttt cgaagcttta aaaattatcg cagtggtgaa
     8101 ttggttcacc gtgatgaaaa accatacatg ggaaacacac tttatattgg ctcattaaaa
     8161 agtgaagtct atttctgtat atatgagaaa gattatgagc aatacgtgaa aaatgatgtt
     8221 cccttagaag acgcagaagt aaagaatcgg tttgaaattc ggttaaaaaa tgaccgagcc
     8281 acccaagcca tgaaagattt attggcacat caacaggcgg aaaaaacagc ctttgaaatt
     8341 atcaatcgct atatccgctt tgcagataaa gacgacacta aacgtcggag tgattggaaa
     8401 actaatgaac gttgggaatg gtttatcggt aaaaatcgtg gagcattacg cctaaccaca
     8461 cagccagagc cttattcttt tgaacgaaca ttgaactggt tacatcatca agtcgcccct
     8521 acattaaaaa tcgcaagcat tttagatgtt ctcaacggta caaccattat ctccaccatg
     8581 attcaggaag ccaaactgac agaaaaacat gagaaattaa ttgaacaaca acatctagcc
     8641 atggaggatt tgattacatg agccttttaa caaaagaatt aaagaagtta gggtttcagt
     8701 gtggcattga gtttcaagcg tatatacaaa atacagggaa atatacgtct ctcattattg
     8761 agggaaaacg gcaagctggt gataccatct atacctatga tttttacaag gtgagatttt
     8821 ataataatta taccaatcga gtaaccgtct atggagagca tttaacccct tttcaattat
     8881 taaggcgtgt aaaaagctat atctattatc gagaaaaata tcttaaggaa aggaggacaa
     8941 tcacatgaat tttgggcaga atttgtacaa ctggttcttg gataatgcac agtcattggt
     9001 attgttagcg attgttgtca ttggactgtt tttaggtttt aaacgagaat tttcaaaact
     9061 cattggcttc ttgattattg ctctaatcgc cgtcggactt gtgttcaatg cagctggcgt
     9121 gaaagatgtg ttattaaact tatttaaccg aatcattggt gcttaacaga tatttttcat
     9181 gataaaatag gaagtatata ttttgttatg aaaggattta tgaatatgtt tacaaatgat
     9241 caatttaaat ctcttttaat taaaacatta gaaagaaaga gctcttcaaa ttattatgaa
     9301 ggaggtaacg agattgtttc taaaatgaaa atatcagagg taactttaga tgagactgac
     9361 gattttactt attataaagg atataaaaag ggctggaata cgctttgcac atatcttaaa
     9421 ataagagttc cttttgatga tttagacttt tttgaaagtc ataaagattt gattacccag
     9481 acagcaagct ccatatatga taaacaaggt gataatgtat tagtggatac tattttagta
     9541 ccacttccag agaattacga agtaattaac tttagtcagt tgaaaattag tgatgtggtt
     9601 tcacaagcta ttgaagacgc tgagtctttt atgagtaatg gtgaatatca acgtgccttt
     9661 gacagagttc acactgcttt tcatggatat ttaattgaaa tactaaaaaa atatgaaatt
     9721 acagttccta gagacgaaaa tctatctaaa ttatatagta ggatacaaca attaattgaa
     9781 aaagaaattc aacctactga acttgctgat atagtgaaga caacgataag gtcatcaaat
     9841 ggaatgatta gctctcttaa tgaagcaaga aataggcatt cacttgcaca ccctaataca
     9901 aatattattg gaaagagaga agcaaaacta ataataggta tttcaagtac agttacagat
     9961 tatattagcg gataccttga taagtagcgt cattaactga ttgctacttt ttttatgact
    10021 atttttataa aaaggatgtg ataagaattg gaaatgaaag tctatgtggc aaatttagga
    10081 cggtataatg aaggtgaatt agtcggagca tggtttactc caccaattga tgaggaagaa
    10141 atggcagaaa gaatcggttt aaatgaggac tacgaagaat atgcaataca tgattttgag
    10201 cttccttttg atgtggacga atacacgccg attagtgaaa tcaaccgtct atgtgaagcc
    10261 attcaagaaa tcgaaggtac acctatttat aatgaactca aagaaattca aggcatgtgg
    10321 tttagtagct tggaagaatt actggaaaac aaagaggata ttcattgtta tagtgattgt
    10381 gattcgatgg aagatgtcgc ccgttattac gtggaagaaa caggacagct tggtgaagtc
    10441 ccttccaatt tacaaaatta tattgactac caatctttgg gacgtgatat ggaaatagag
    10501 ggtaattacc tagttacctc tcatggggta tttgaatatt gtcagtaagt ttaacaatcc
    10561 gtgttataat taaattaata aatagcacgg agaggtataa attatggtaa atgtatttga
    10621 aaatcttaaa ctattaaaac tagacatgaa agataaaggc tgggtgatag attcctttta
    10681 ctttcgttac aaacagcaaa attatattgt acttgttaag ctatttgaaa aagaggaaaa
    10741 agttccagaa tatgctctgt taaaactaga atttttaaag gaaaacgatt tttcagatat
    10801 gctagctgtc tatgctaatt cggtaaaatt atatacagac acgaaaacaa taagagaata
    10861 ttttggtatc gaatacagta gtaacttggg agatgtttta tttcaattta gccaaactct
    10921 tgctcgattt attccgacag aagttagtga gaagaaaaat gaagaccaaa aagaagccat
    10981 gtgtttctca ttaagtcaaa gtgattcaga agatcccaga aaaaaatatt gtttttcagt
    11041 tagaagaaat cctttaaagg gaaatggtga gctgggaaaa cgaagtcctt ttaatgacaa
    11101 taaaacgaga ttgtatagac ccagcttata tgaacgatta gggtcagata ccaatttaag
    11161 tttcagatat tcaatgaatc cagatgatga agaaactgat gaaggtatta ttgcaaaatg
    11221 gacaaaaaat aaaattgaat agttaaagca ttgaaagagc aatcacctat tttgattgtt
    11281 ctttttctat ttttacggaa aggacgtgaa gacatgaaaa aaataagaag ttacaccagt
    11341 atttggtcag tagaaaaagt gatttatgcg attaatgatt tacaactccc tttcccaatt
    11401 acctttacgc aaatgacatg gtttatcgtt tctttgtttg tcgtcatact ctttggcagt
    11461 ttaccaccac tttctctgat tgacggagca ctactcaaat acttagggat tcccattgtt
    11521 ctcactgggt tcatgtgcca aaagaccttt gacggaaaga aaccctatgg atttctaaag
    11581 tctatcatta cctatgctct acgccctaaa gtaacctacg ctggcaaaca aatacgatta
    11641 aaaaaagaaa caatgaatga atcgattacg attgtaagga gtgaacaact agatggcata
    11701 tccgattaaa tatatggaag ataatctcgt atttaatcat gatggcgaag tattcgctta
    11761 ttatgaatta attccctata attacagttt tttaagttcc gatgaaaaaa ttcaagttca
    11821 tgataatttc tgtcaattga ttgcccaaaa tcgtgatggc aagattcatg ctctgcaact
    11881 agcaaccgaa tcaagtattc gagccacaca ggaacaatcg aaaaaagaaa tcactggacg
    11941 attaaaagag atagcttacg aaaaaataga tgaacagacc gaagcgttaa tttctatgat
    12001 tggtgacaat caagtagact atcgcttttt tattggtttt aaactctacc tcaatgaaca
    12061 ggaagtatcc gtaaaaaatg ttgggaaaga aattcaaaaa gcatttgcgg aattctttca
    12121 tggtgtgaat cacaaattaa tgggcgattt tgtttctatg cctatggcgg aaatcgaacg
    12181 cttttctaag atggaacagt tgttagaaag taaattatcc agacgctttc agattcgtaa
    12241 attggagaaa gatgattttg gttatattat tgaacatctt tacgggcaaa caggtgtccc
    12301 ttttgatgac tacgactacc atttaccgaa acgatacgaa gataaaaaag tgttggtaaa
    12361 gaaatacgat attctcaaac ccactcgctg tttaatcgaa gaaaatcaac gctatttacg
    12421 gattgaacag gaagatacca ctacttatgt ggcgtatttt acaattaata gtattgttgg
    12481 tgaattggat ttccctaata gtgaaatttt ttattaccag caacagcagt ttactttccc
    12541 cgttgatacc agtatgaatg ttgaaattgt cgccaataaa aaagccttat ctaccgttcg
    12601 caataaaaag aaagaactga aagatttaga tagccacgca tgggaaagcg acaacgaaac
    12661 cagtactaat gtggtggacg ctttagaatc tgtcgatgaa ttagaaagta atttagacca
    12721 aaccaaagaa tctatgtaca aactaagcta tgtggtacgg gtatcagcaa agaatttgga
    12781 cgagctgaaa caacgttgtg atgaagtaaa agatttttat gatgatctgt ctatcaaatt
    12841 agtacgacct tttggcgata tgctaggctt gcataatgaa tttattccct caagcaagcg
    12901 atatatcaat gactatattc aatatgtcac gtcggatttt ttggctggtc ttggtttcgg
    12961 tgctacacaa atgattggtg aaaattctgg tatttacgtc ggttacaatc tcgatacagg
    13021 cagaaatatt tatctccaac ctagccttgc aagtcaagga attaaaggct ctgtaacaaa
    13081 tgcattagcg tccgctttta ttggttcatt aggtggcgga aaatcattct ctaacaatat
    13141 gctggtctat tattcagtac tctttggcgg acaggcaacc attgttgacc caaaagctga
    13201 acgtggtgaa tggaaagaaa ccttacctga tattgcccat gaaatcaata ttgttaatct
    13261 cacgagtgag aacgacaata aagggttact tgacccttat gtgattttaa aactcccgaa
    13321 agattcagaa agccttgcca ttgatattct cacgttctta acaggtattt ccagccgaga
    13381 tagcgagaag tttcctgtcc tgcgtaaagc tattcggagt gtaactcaaa gcaaacaacg
    13441 agggttgtta ttggtgattg atgaattgcg aaaagaagat acgcccatca gcaatagtat
    13501 tgctgaccat atcgaaagct ttgtcgatta tgattttgcc catctgttat tcagtgatgg
    13561 cagtgtcacc caatccatta gcttggaaaa acaactcaat attattcaag tggcagattt
    13621 ggtcttaccc gattccaata gctcttttga agaatacact accatggaat tattaagtgt
    13681 ggcaatgctg attgtcatta gtacctttgc tttagacttt atccactctg accgttccat
    13741 ttttaaaatt gttgaccttg atgaggcttg gagtttttta caagtggcac aaggaaaagc
    13801 tctatctatg cgattggttc gagcaggacg tgccatgaat gcaggggtct atttcgttac
    13861 tcaaaacgcg gacgatttac tagatgaaaa gatgaagaat aatattggct tgaaatttgc
    13921 ctttcgttcc actgacctag tggaaatcaa aaagacctta gaattttttg gtgtggacaa
    13981 ggaagatgaa aacaatcaga aacgcttacg tgaattagaa aatggacaat gtttagtttc
    14041 agacatctat ggtcgtgtag gggttatgca gtttcacccg atttttgaag agttgcttca
    14101 tgcctttgat accagaccgc ctgtgcgaaa tgaggtggaa gaatgacaca aaaacaaaag
    14161 aaatttcttc actatagttg gataacgctc ctttgtgtgg gcgttatttt acttttatta
    14221 ggcacgctgg gcaatgcagt acaagcgaca ggcttagttg atgaaaccat tgatacaagc
    14281 aatgaatact ccaagtatgg tttgaatcaa tatcagcttg attattatgt ggacaatagc
    14341 tggggctggc ttccatggaa ttggagtgat ggcataggac aatcggtgat gtatggattg
    14401 tatgccatta ctaattttat ttggacaatt tctctttatc tatcgaacgc cacaggttat
    14461 ttggtgaaag aagcctatgc cttagatttc attagtcaaa catcccaagc aattggcgaa
    14521 aacatgcaga cgattgcagg cattacaccg aatggctttt ccagttcagg gttttatgtc
    14581 ggtttcctcc ttctttttat tctcattgtt ggaatctatg ttgcctatac aggattggtt
    14641 aagcgagaaa cgacgaaagt gatacgagct gttttaaact ttgtcgtggt gtttattcta
    14701 tcggcttcct ttattgcgta tgcacccgat tatattgcga aaatcaatga tttttccaaa
    14761 gatgtcagta ccgccagtct gaatattggg acaaaaattg ttcttcctaa cacggatagt
    14821 caagggaaag acagtgtgga catgatacgt gacagcttat tctctgttca agtaaagcaa
    14881 ccatggctat tgcttcaata tggctccact gatgtagaag cgttagggga agaacgtgtg
    14941 gaaagtttat tgtctaccag tccagataca aacaatggtg aagatagaga aaatgtagtg
    15001 attgaagaaa ttgaagatca tgacaatacc tatttaaccc taccgaagac gatttccaga
    15061 ttaggaacag tcttcttttt gtttgtattc aatattggta tctcgatttt tgttttcctt
    15121 ctaacaggaa tcatgatttt ttcgcaagtg ctatttatta ttttcgctat gtttttgcca
    15181 atcagtttcc tattaagtat gttgccgagt tttgatggca tgggcaggca tgccattacc
    15241 aagttattta atgtgattat gacaagggct ggtattacct tgattattac cgttgccttt
    15301 agtatttcca ctatgcttta ttcgctgtca gcaagttctc cctttttcat gattatgttt
    15361 ttacaaattg tgacctttgc agggatttat tttaaactgg gcgatttaat gagcctattc
    15421 actttacaaa gcaacgactc tcaaaacatg ggcagacaag tgtttcgcag acctcgtcaa
    15481 atgatgaatc gacaaaccag aagatttcaa cgcagtatga gtaagatttt tcgtggaaaa
    15541 gcagggtcaa cgaagcaaaa gacaatgaca acaaagcaga atcaaaccaa taaagctaac
    15601 catactcgtc aaaatgaacg gactacacca aagaaagaaa cagcactttc caatgtgaaa
    15661 aagcaacaag gcggtatcgg gcaacgatta ggtgaaaaga cagcccatgt attggacacg
    15721 aaaaatcgtc tagtcaataa aagaaagcag gcaaaacaac agatacaggc gacaccaacc
    15781 catgtcaaat atgcccttca tcaaggaaaa gagaatgtca ctcataatgt aacagacttt
    15841 aaacaatcta tggcagatac caaagctcaa cgcaaacaac agcgtatgaa ccagcaggag
    15901 caaaaacgaa agaacattgc tcaaaagcgt ttggagctgg ataaagcaaa agaaaaaaga
    15961 cgaaaagaaa acccatcatc tcctgttcag aataaccagc agcatgttcg caagcgtcag
    16021 caaaccgtca cgcctgtttt gtcacaaaac actaaacgtg aaaatcatca gcgaccattg
    16081 aaaaaagagc aaaaacaaaa gacaccaccg aagcaattca atcagaggaa gctaaataaa
    16141 agaacggata agcgaggaca acaaacatga agctaaaaat aacagtgatt tgtagtgtgg
    16201 tcttctctct cttctttttt ctcttattat tcctagtcat tttctttgca gatgatacgg
    16261 acagtggcga aaacaacaag gattcttcta ttccacaagg cggtgtgacc gtttcaccag
    16321 aagtgttggc acaccgtccg ttgattgaaa aatatggtaa ggaatatggg attgaagact
    16381 atgtttctta tatcttagcc attatgcaag tggaatcggg cggtacagca gaagatgtta
    16441 tgcaaagttc cgaatctttg ggtttaccgc ccaacagttt gagtaccgaa gagtccatca
    16501 aacaaggggt taaatatttc agtgaattat taaccagtgc agaacaacaa ggagtggata
    16561 tagattctgt tatccaatct tataattatg gcggtggctt tttaaattat gtgaggagtc
    16621 atgggaaaaa atatacctat gaattagccg aacagttttc taaagagaag tcgggtggtc
    16681 aaaaagcaga ctatcccaat cctatagcca tacctgtgaa tggtggttgg cgatataact
    16741 atggaaatca gttttatgtt cagttggttt cacaatacct aaccgacact tccccaacag
    16801 aatttgatgg tgaaaccgtg caagtcatta tggacgaagc cttgaaatac gaagggtttc
    16861 cctatgtctt tggaggggcg tcgcctacca cctcttttga ttgtagtggc ttaatacaat
    16921 gggtgtatga caaagctggc atttctctcc ctcgtgtcgc ccaagatcag tatgacgcta
    16981 cgcaagaaat ctctatggaa gaagcccaag caggcgacct tatcttcttt cattcaacct
    17041 ataatgcagg cacatatgtg actcatgtgg cgatatattt agagggaaat cgcttttatc
    17101 atgcaggcga cccaatcggt tatggcgact taagcagtcg ttattggcaa gaccatctga
    17161 ttggggctag acgtgttatt cataactaag aaaggaatga atcaaatgat tataaaaatc
    17221 gagcgaaaag aaaagaagga gaaaccaaag aaatctaaaa aaccgagcaa gcaaagaaaa
    17281 gttccgatga ttaaagttgg cacgcataag aaattaacct ttgttttatg ggttttattg
    17341 attggtagtg tcggatttgg aatctataaa aattttactg ccattgacac tcatactgtc
    17401 cgtgaaacag aaatcattaa acagcagatt gtcgatacca accaagtaga aagttttgtg
    17461 aaatcctttg cgaaagacta cttttcgtgg caacaatccc aagaagccat tgataaacga
    17521 aatgaaaaat tgacccatta tttgacggaa gaattgcagg tattaaatga agaaatgatt
    17581 cgtaaggata ttcctactag ttctagtgtg aacgatatac aggtgtggca ggtttctcaa
    17641 gtgaatgaga acacctttga ggtactgttt tctgttgaac aagttattac cgaagacaaa
    17701 gacaaggaaa ctatatcgtc tagctttcat gtggttgtcc atatagatga atcagataat
    17761 atggttatta tcaaaaatcc gacaatgagt aagaaacctc aaaaatcgga ctaccaaccc
    17821 aaacagctag aaagtgacca tacagtagat acagaaacga tggacgaaat cactagcttt
    17881 ttagaaacgt tcttccagct ctatcctacc gccacagaaa aagaattgac ttactatgta
    17941 agtaatcatg tgttacctat gattaacaag gaatatgttt tcgaggaatt ggtgaatcct
    18001 atctttacca gaaaagataa ccaagtaatc gtgaatgtcg ctgtgaaata tttagatcaa
    18061 gaaacaaagg caacccaaat ctcgcaattt gagttgatat tagaaaaaca agataattgg
    18121 aagattgtaa aataacaaat attggtacat gattacagat actttgtaat catgtactct
    18181 ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagtg
    18241 actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
    18301 atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt tgtacccagt
    18361 ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta tgctttgtat
    18421 acctatggtt atgcataaaa atcccagtga taaaagtatt tatcactggg atttttatgc
    18481 ccttttgggt ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc
    18541 tcatgttgat gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat
    18601 tacagaatta ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg
    18661 tcagagagga attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt
    18721 gaacatcata gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc
    18781 agttttagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg
    18841 tatattattt catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat
    18901 tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc
    18961 cgaaattgta atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac
    19021 cgaatctgaa caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat
    19081 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
    19141 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
    19201 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
    19261 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct
    19321 ttatagtgga gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa
    19381 agttacagaa atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc
    19441 tggagaaatt gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac
    19501 aaaactattg ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt
    19561 tgaaccgagt aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga
    19621 tagtgatccg cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt
    19681 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga
    19741 gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata
    19801 taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc
    19861 accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa
    19921 tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata
    19981 tggttggaat gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt
    20041 tagtactcca gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa
    20101 agctggaaca gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata
    20161 tctttcacgg gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact
    20221 gaaaaataat gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg
    20281 caatgattta acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata
    20341 tcaggttacc actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa
    20401 agtaagatat atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt
    20461 ttcttattaa ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg
    20521 agattgttat tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac
    20581 tacgaatacg agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga
    20641 acgaaacttt aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca
    20701 agacgcagag ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat
    20761 ctgaatttta ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct
    20821 tccattaagg atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt
    20881 cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt
    20941 aatgatacaa gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt
    21001 gttaaataac gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt
    21061 ttcattcgag cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt
    21121 ttacgcatac taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat
    21181 agaaaaacat gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg
    21241 atgaatccaa tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa
    21301 tttagtcata ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca
    21361 tgagaaagat aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc
    21421 acctgtactg gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat
    21481 caaagcatat gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata
    21541 tcttttagga tttctcttca tttatgcaac aaatccccct ttcgtgtcgg ggtttagtat
    21601 gaagggagat aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat
    21661 gaaaccatct tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt
    21721 tattgaagat gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt
    21781 ctcgttttct gatatagata actaccttgt caactctttt tctactgttg atacgtacgt
    21841 cactgatttc caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt
    21901 aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt
    21961 tatggatatg agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta
    22021 tcgtcatcga ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg
    22081 aaaacagctt atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg
    22141 gaagcaatta atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt
    22201 cgcatgacag acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga
    22261 atggaaacga aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc
    22321 gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa
    22381 agcatattgt gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc
    22441 aaataaaggc aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga
    22501 cctacaaaat tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc
    22561 tgccatgact ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga
    22621 agcgtcacag gca
//
LOCUS       DAYLZO010000364        11018 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.094, whole genome shotgun sequence.
ACCESSION   DAYLZO010000364 DAYLZO010000000
VERSION     DAYLZO010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.094"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(134..577)
                     /locus_tag="ACGVJG_003341"
     CDS             complement(134..577)
                     /locus_tag="ACGVJG_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFJ6559202.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACGVJG_003342"
     CDS             complement(795..2135)
                     /locus_tag="ACGVJG_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFJ6559203.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACGVJG_003343"
     CDS             2404..3300
                     /locus_tag="ACGVJG_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFJ6559204.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACGVJG_003344"
     CDS             complement(3450..4391)
                     /locus_tag="ACGVJG_003344"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFJ6559205.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACGVJG_003345"
     CDS             4517..5638
                     /locus_tag="ACGVJG_003345"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFJ6559206.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACGVJG_003346"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACGVJG_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFJ6559207.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACGVJG_003347"
     CDS             7465..7992
                     /locus_tag="ACGVJG_003347"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFJ6559208.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACGVJG_003348"
     CDS             8203..9264
                     /locus_tag="ACGVJG_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFJ6559209.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACGVJG_003349"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACGVJG_003349"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFJ6559210.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..>11018
                     /locus_tag="ACGVJG_003350"
     CDS             10177..>11018
                     /locus_tag="ACGVJG_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFJ6559211.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLS"
BASE COUNT         3699 a         2010 c         2266 g         3043 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttc
//
LOCUS       DAYLZO010000365         7398 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.128, whole genome shotgun sequence.
ACCESSION   DAYLZO010000365 DAYLZO010000000
VERSION     DAYLZO010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.128"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(202..1395)
                     /locus_tag="ACGVJG_003351"
     CDS             complement(202..1395)
                     /locus_tag="ACGVJG_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFJ6559212.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(1474..1680)
                     /locus_tag="ACGVJG_003352"
     CDS             complement(1474..1680)
                     /locus_tag="ACGVJG_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFJ6559213.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(1673..2815)
                     /locus_tag="ACGVJG_003353"
     CDS             complement(1673..2815)
                     /locus_tag="ACGVJG_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFJ6559214.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            3327..3962
                     /locus_tag="ACGVJG_003354"
     CDS             3327..3962
                     /locus_tag="ACGVJG_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFJ6559215.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            4098..4190
                     /locus_tag="ACGVJG_003355"
                     /pseudo
     CDS             4098..4190
                     /locus_tag="ACGVJG_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            4304..4939
                     /locus_tag="ACGVJG_003356"
     CDS             4304..4939
                     /locus_tag="ACGVJG_003356"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFJ6559216.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            5046..6284
                     /gene="mef(H)"
                     /locus_tag="ACGVJG_003357"
                     /gene_synonym="mefH"
     CDS             5046..6284
                     /gene="mef(H)"
                     /locus_tag="ACGVJG_003357"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFJ6559217.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            6511..6930
                     /locus_tag="ACGVJG_003358"
     CDS             6511..6930
                     /locus_tag="ACGVJG_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFJ6559218.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            6935..7174
                     /locus_tag="ACGVJG_003359"
     CDS             6935..7174
                     /locus_tag="ACGVJG_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFJ6559219.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         2215 a         1291 c         1395 g         2497 t
ORIGIN      
        1 ggcacctact ttaattaaga gtcataatta ttataaatta tatatgtctt tttgactatc
       61 tttttatatc ttctcaaatg tccgcaaagc cttattttat cggctctacg ggcattttgc
      121 ttttgtggta aacctcgcat atctaggtct atcttcttat attttccctg tcaatcgtgg
      181 ttaaaatcgt ggtaaatact tctatgcgat tagacgctga acctctgatt ttgcggtatc
      241 tatggacgca tgagcgtacc agttcattgt gatactgatg tttgaatgcc ccatgatata
      301 ctgtaaatct ttcgggttca tgttcttgct tgccagcctt gtgcagaatg tatggcgtag
      361 cgtatgcggt gtaatatgtg gcaagggatt gtccttgtgg tgcttgttgt atttctttac
      421 catacggata aataaggcgt tataatcaat ggcaactttt ggattacctt tcggattgat
      481 aaacacaaaa ccgctgtgtc cgtctacctc tatcgacttt atctttggtc ggctgttgat
      541 aacccgttga aatgcctgta tcgtttcttt gcttaatggc acttgcctta tcccgctttt
      601 cgtcttaggc gtttcaatat aatagccctg ttccttactc tttagtaact ggtggtcgat
      661 aatcacaact tcattcttga aatcaatgtc ggctactgtc agtccgcaca gttccgagat
      721 acgaagtcct gtctttaaca gtatcagcac atcatcataa tgcttgtgat acacgttgtc
      781 cgtcttaata aatgacaata aggcttgctc ctgttcctct gtcaatgcga ctttctcttt
      841 ggtatcattt tctatgactt cacttagctt gaaatcaaaa gggtttttcc tcacataatc
      901 atcttgtatg gctatgtaga atgacgcttt taacgagcgt ttatggttgt taatggtatt
      961 gtaggaaaag cctttgtctt tcatgcgtaa cgcccattct ttagcgtcag agggttttat
     1021 cgtatcaatg ctcctagcac ctaacttgtc ctcttttaat aaccgcatga gttgttcccg
     1081 ttgtttctgt gtgcttttct tcacatttgt cctctgtgcg ttctgtttgg catagagttg
     1141 gcaaagcgtc attttcttgc ctgtgctgtc gataccgtcc tcaatatccc gtcttatctg
     1201 ctgttccagt tctcgaagtg agggtttttc ccgttttccc tttggtgtcg ggtctgtggg
     1261 tgtcaatctc caagcataca catattttgt gtttcccaat gcgtctacat atttatataa
     1321 gtattttccg tctgttcgtt ggctctctcc agtatgcagg atacgacctt tgttatcccg
     1381 tcttttttct ttcatggtgt ctgctccttt ccttgttgga aagagccttg atatgacttg
     1441 tgttcatcat aacacataca aggctcattt gcattagatt gcgtccaatt tatcaataac
     1501 cttttcaaac tgtcggcgtt taatctgtat gcggttgcca ttcataatga gccagccagc
     1561 gtccttgttt tcctctgcca atcgtcttaa cttgttttct ccaatgcgga aatactttga
     1621 cgcttcttca atggtaaggg tgtatttttc ccatacggga atatcctgtg tattactcat
     1681 aaaacacccc ctcactttcg attacgtctg atatgtccgt acacatctgt tccacaagca
     1741 tttcctgttt ttcagaaagg tgggtacatt catcaatcat tttcatgtag tttgaacctg
     1801 tcagcctgtc gatttcctct atcatcacga ttgacggcat gacctgtctt tccagccatt
     1861 gcagagtgcg ttccatattg acaggtacag ggttcatttc aaaaggtaaa ctgtcatggg
     1921 aaatgaatat ctcccacaat ggatagtcag gaaattccac aaaatcggta atgagataaa
     1981 acaccagccc atgagggtta tacgtcaata acagttcttc aactgcctgt accgcctttt
     2041 tgtcacgcag acgaatttca aaacggttga taatatcggt atgtttcttt ttcgttgcct
     2101 gttccttttg tttttcatac aggcagaaat attttgtgct tgcctttgag ccgatataaa
     2161 gggtacttgc taaattacgg ttttttccgc ttaattttcc accttgtaca ttttcatagt
     2221 ttttacagcg acattccgca ccaccatttc tgtatttttc agatagcaca ggaatatcca
     2281 gcagaccgca catatcattg attgccaagt caaaacgtcg gataacaccg ccacagtcaa
     2341 gacagcggtt taagaatgaa taccagtccc tgttctgtgc ctgtaagaca tattccatgt
     2401 tgcgtgagcc tgcccctttt aattctaaaa atacgcccat gtgttcatca tcagaagcca
     2461 tgaccttgat ttccccgtaa gcataatgct ccttatagcc ataataccca tagtcataat
     2521 ggataaaata atctgctttc atgccaagca cctttcggat aacttccaat gcgtcagtcg
     2581 ttggaaaacg gacagacaga taatcaacga gcagataaaa aggcttatcg cagatatagt
     2641 aattcagaca acgcaggata atgttctgtg tttccttgct ggcgttagct tttccgttct
     2701 caaagcggtt gatactatat cttgatacat gggtaagact tgcaagttct ccttgtgaaa
     2761 gaccgctggc aagacgtttg ttcttcattt caagtgcaaa ttctaaattg ttcattgtat
     2821 tcctccaatc ccacaatagc cttgaaatac ctgtaaacca cctttcaaaa agtgtgatat
     2881 gtactatctt cttaaaacct ctcattacta ctcacaggtt tgggggtgta tggtttttga
     2941 cttgtctttt gtgaaatagt gcccccctgt tagataacgg gggcacagac aaggctcgca
     3001 ggctcgccag ccgacaaagc atttcttccc cttgacacaa ggtgtcatgg cggtaagcct
     3061 gagaggcttc cgccaccgtg tcagaaatac tttgtcagca agttctgttt tgccttttct
     3121 aacaagttcc atttcaaccg atattcatat ctacggattt aatcagatag gtatttcttt
     3181 ttgaatgttt ttatatttca ttctattgtg cttattcgat tacaagaaca tagtaattct
     3241 tatatgctta tttgtcaata tctttttttg cctttacaaa tcatattaag catgatagac
     3301 ttataatagc gaagatataa cgagatatgg agggttatgc aatgaaaata agccatttag
     3361 gaaataatat acagaccata agaaaattta ggggaatgaa acaacaggaa cttgcggaca
     3421 aaatcggtat caatatgcag agcctttcca agattgaaag agggttgaac tatcctgcct
     3481 atgaaacgct ggaaaagata atggaagtgc tggacgttac gcccaatgaa ttgttgtcgg
     3541 gagaatggaa gtatgtcaat caatctgaaa aggaagtctg tcagttttta agagccgaag
     3601 aacgtctaaa tgcagaactg aaacatggac actatgataa cttctttgac agcgaggaag
     3661 aatggctgga atatgagtta gaaaagttac gggaatatat tacagactat atcaacggga
     3721 aaagcattga agcgtccgac ctttacccaa tcaaagaatt tatccagcat ttgaagtttc
     3781 aaaaattatt agaccgctat gatgatttat acagtatgga tatgtttggg gaaagcatag
     3841 agggacataa gtataagaca ccttatcaag tcgtgaaaat gataaacccg aactcaaaag
     3901 aagatatgga actgttacga gaagtattaa ggaataacaa ttttgatgat gaggacgagt
     3961 aacttttttc ctcttgcgag aagatacagc aaacaaaaag cccgatcaag agtgcaaagc
     4021 accaccaatg gtggcaaagc tcttgacagg gctttttctt ttctgttgtg ggcaatcaag
     4081 aggaagaaag ggataaaatc tgatttgtgg taaatcaggc aaaggcaacg caggtatcac
     4141 agtatgaatt gacaccgcat aaggaaagca actggaagat tgtagaataa atgaaaaagg
     4201 cttgcattct gcatttttat aaaattgcaa gtctttatat tgacaagcat aaaatactaa
     4261 gatatacttt aatcagacca acggtttgta ttggaggtaa gttatggcac gaaataaata
     4321 tccagaagaa acagtaaaac tcattttaga tgtttctaca aaaatgtttt gtgaaaaagg
     4381 atatgataac acatcattgc aagatattat caatgaaaca aaactttcta aaggtgctat
     4441 ttatcatcat ttcgattcaa aggaagatat tttaaaagca atttttcaaa gaattggaaa
     4501 tgaaaatgca gtaatatttg ctaaaattag agataacgat agtttaaacg gtttagaaaa
     4561 gttaagagaa atatttaaga cagcggtatt caattctaac caaagcgttt tattgacagt
     4621 ttctcctcaa ctattaaaca atcctcgttt tcttgctatg caaatagaac aaatgtatga
     4681 aattgtagta cctgaatttg ttgaacccgt tttagaacaa ggtatcaagg ataagtcttt
     4741 ggagattgaa aattcacatg aaattgcaga agttatcatg gtattaagta atgtatggct
     4801 caacccatta gttgaaatga cggataaaga gggtatggaa aaacgttgca atacattcaa
     4861 tgccctttta aatggaatag gcatagacac attattagac gaagaaacaa tttctgcatt
     4921 tatacagttt tgtaaataac tgtatttgtt atccctatac gatagactgc tctataaaag
     4981 agcagtttat ttaaaccatt atataaaccg acggtctgtt tgattaaata aaaaggagag
     5041 attttatgca agcagaaaaa aaacttttta atcgtgattt tatacttgtt gttataggac
     5101 agataatttc tttatttgga aacgctatcc tacgatttgc attaccgtac tatttgttaa
     5161 aagaaactgg ttcatcagct ttgtttggta ttgctacagc ctgctctttt ttacctatgg
     5221 ttattttgtc gctggtaggt ggtattattg ctgacaggac aaataaaaga aacattatgg
     5281 tcatactaga ttttgtaaca gcaatcataa ttttagtatt ttatttgtca ttgggaaaaa
     5341 ttcctatcat acctttgttt attgttgctt tgatgttact ttatggtatt tctggtgcat
     5401 atcaaccagc ggtacaagca agtattcctc tattggtatc atcagataaa cttaccgtcg
     5461 gtaatgctat aataaatcaa gtaaatactt tagcaaactt tatcggacca gctataagcg
     5521 gagtagtttt tcaattttat ggtatcaatc ccattttaat tgttagtatt atatgtttta
     5581 ctttttcagc cattatggaa atttttatcc atattccaca ccaaaaaaga cctcgtgaag
     5641 taggtgtaat tgcagttgct aaaaacgatt taaaagatag ttataccttt ttgaaaaagg
     5701 agaaacccat ttttatatcc gtaatctttt tggtttgcat tttcaatttg gttttaagtg
     5761 ctgttttaat tgttggtctg cctatcttaa tttctgaagt tttaaaaatg ccagatacac
     5821 tatatggttt ttcacaatca gcattatcat taggtggttt atgtggtggt attttaacag
     5881 ctcttgttgc ttcaaagtta aaactaaaaa acctttatac cttgttactt atttgttctg
     5941 tttctgtttt acttatggga atttcgctat ttttagaatt gcctgcaata gtttcctact
     6001 gggtagttac agtgatgtgc tttgtggcta tgggtgtatc tactatgttt gttgttcaaa
     6061 tttacactct tgttcagaca caaacaccac cgcaattagt aggaaagata atggctacgc
     6121 tggtagcagt tgctatgtgc ggtcaaccta ttggacaaat ggtttacggt attttattcg
     6181 atgtatttat acaagaaaca tgggttgtta tgtttgtagc ttctattgct tcaatcatga
     6241 tttcaatata ttcaaaaaga atttttcaaa agttagagat ataatacaag gggttaaata
     6301 gtatgttagg ctaaaaacaa tatataaaag aaaatttgaa atctgctaaa aggatagtgt
     6361 tataaaagaa agagtaatat tgcagtattt aaggctcgca caaatatatg ttttgtgtgg
     6421 gcttttttct taccattttt ctgtatttct aaagggtata aacggtattc cgtttatata
     6481 tttcagcatt agcagaaagg gggtgagatt atgaggacat cttctttcga gcatatcgtc
     6541 agattacagt tcaattcttt aatgctgatt gtcattaaga acaaagtgaa aagtcgaaac
     6601 agacagcttg caagacattc taaacgtgaa gtattgtttt gtgaaatagc cgaaacgaaa
     6661 caagttgaac gtggaacgat tgatacctgt ttttatgatt gtgtatcatt taaagtcttg
     6721 cactttacaa tttatgtatc tgatgaaaca cttggtacag ttttacatag actgtcagaa
     6781 aaacagcgta gtgcgatttt attacgttat tttcaaggaa tgaacgacag agaaatttca
     6841 gaactgtacc atgtatcacg ttctgctatt tgcagtagaa gaactagagg actaaggaag
     6901 ttaaaaatgc ttttgagcga aaggaagtaa agaaatgagt aaagattatg gctatccctc
     6961 ttttgaattg atttgcaaag cgtcaagcgg agatgaaatg gcagttaaag agattttgaa
     7021 gttttatgac ggttatattt ctaaattatg cttgcgtcct ttttaccact ctgaaagtgg
     7081 taaaatcatt atgcaggttg atgaagaact aaaaggagaa attcatacag acatgatgaa
     7141 agcaatattg aaatttgaaa tcggagtgaa gtaatcaact gtataaaaaa atcgggagat
     7201 acacagtttc gtgctatctc ccttagtagt tttacggtca tatcaaggct cattcaatcg
     7261 tggtaaattc gtggtaagat gagtgttttt ctatacttca aaatgcttga atgtatagtg
     7321 tttatgcgga taattgaaaa ttagatataa aatttcaaat aaattactga tataaattta
     7381 caataagaac tcttgttt
//
LOCUS       DAYLZO010000366         6276 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.143, whole genome shotgun sequence.
ACCESSION   DAYLZO010000366 DAYLZO010000000
VERSION     DAYLZO010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.143"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            110..193
                     /locus_tag="ACGVJG_003360"
     CDS             110..193
                     /locus_tag="ACGVJG_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFJ6559220.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACGVJG_003361"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACGVJG_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFJ6559221.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1225..1485
                     /locus_tag="ACGVJG_003362"
                     /pseudo
     CDS             1225..1485
                     /locus_tag="ACGVJG_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1648..2313
                     /locus_tag="ACGVJG_003363"
                     /pseudo
     CDS             1648..2313
                     /locus_tag="ACGVJG_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2349..2594
                     /locus_tag="ACGVJG_003364"
     CDS             2349..2594
                     /locus_tag="ACGVJG_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFJ6559222.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2697..3566
                     /locus_tag="ACGVJG_003365"
     CDS             2697..3566
                     /locus_tag="ACGVJG_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFJ6559223.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3547..4281
                     /locus_tag="ACGVJG_003366"
     CDS             3547..4281
                     /locus_tag="ACGVJG_003366"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFJ6559224.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4314..5229
                     /gene="ant(6)"
                     /locus_tag="ACGVJG_003367"
                     /pseudo
     CDS             4314..5229
                     /gene="ant(6)"
                     /locus_tag="ACGVJG_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5226..5768
                     /gene="sat4"
                     /locus_tag="ACGVJG_003368"
     CDS             5226..5768
                     /gene="sat4"
                     /locus_tag="ACGVJG_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFJ6559225.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5861..>6276
                     /locus_tag="ACGVJG_003369"
     CDS             5861..>6276
                     /locus_tag="ACGVJG_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFJ6559226.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSR"
BASE COUNT         2225 a          939 c         1287 g         1825 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccat tttgtgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac tgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttac agatgtaata
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ttcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggagtatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttttaaattt
     1081 ggaaagttac acgttactaa agggaatgga gataaattat tagatatact actgacagct
     1141 tccaagaagc taaagaggtc cctagcgcct acggggaatt tgtatcgata aggggtacaa
     1201 attcccacta agcgctcggg accccttgta ggaaaatgtc ctaagtgtgg caacaatatt
     1261 gtattaaaaa aatcgtttta tggttgttca aattatcctg aatgtaagtt tactttagct
     1321 gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa aagaattact ggagggaaaa
     1381 gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc cctacaatgc cgttgtaaaa
     1441 attggggaaa agggatatat tgattttatc tctttttcaa aataaacatg gggctgaccc
     1501 aaaagtagct cctatatgaa aaatagaggt ctttccattt agatagtagt ggaaagacct
     1561 ctatttatct tttgaaagtg aatacaaaaa taaagcacac tctgctataa tataggtgat
     1621 taatccattt gagagaggtg ctttatcatg tatcaaaatt ataccataaa ccaactttgt
     1681 ttgcctatag atcttgaaat taaattagaa gaaaatgatt ttgctcatgc cattgtgcag
     1741 tttgttgact ctattcccga tgaagtcttt cttccttatt atcaatctat gggtagaccc
     1801 caatatcatc ctcgtatgct actttccatt attctttgtg cctatattca aggggtttat
     1861 tcgggccgcc aaatacaaaa tatgctgata gatagtattc ggatgcgcta cttatctcaa
     1921 gagcaattcc ctaactttcg tactattaat cgttttcgcg ttcatccgat tatgaacgat
     1981 attctagatc attcatttgt tcaatttcgt gagctactgg ttcaaagtgg cttaatcagt
     2041 ggcagtgctc tatacattga tggtacaaaa attgaagcgg atgcgaataa atactctttc
     2101 gtttggaaaa aaagtatttt aaagtataag gatagccttg atcaaaaagc ccttgaaaac
     2161 taccaacaaa tggtggaaac aaaaatctta cctgagttaa tagatgattt aaaggatgaa
     2221 ctttctatag aagagattga aaggattcgt caatctttgg aagaaaagga aaatcaatta
     2281 attgaagcga ttgaccaaac cgaaacagta gaagggggct gacccattat taataaggag
     2341 tggacaacat gattgatgta aatgagctat tggaagaagc tattagagaa actgaaaatc
     2401 taaatgatgg cgaagttttt cttgttaaag atttattcaa gggatatgtg gggaatagga
     2461 tacccagaaa ggatcgactt ctgcttggaa cgttattttt aaaccatgtt agcaaaatga
     2521 atggtaattt aaaggctatt gaaaagactt catctaatca gcagaggtat aaaaagacaa
     2581 ttgataagtt ttaactatct tttgtgaaat taaaaaaatt ataaatatgt tgagtagaag
     2641 caaaagattt ggcttggaat ttttacagca gtgaatcgat aaaaggagca tgcttgatgg
     2701 tagataatat tattaaatca gtagcagaga aattatcctc tctgtcttat atagaaggta
     2761 ttgttttagg tggttcacgt gcaaggggca cccatacaga ggattcggat atagatatcg
     2821 gcatctatta caattcagaa tcatttgaca taaatactat taatcaattc gctacaaagc
     2881 tggatgatga gcatagaaat aaccttgttg tacctcccgg agcatggggt gattggatta
     2941 atggcggcgg atggttagtc ataaacgggt atcatgtgga tttaatttta cgtgatatta
     3001 aacgtgtgga acaaataatg aaagatacag agcacggaat tgttactgcc aattatcaga
     3061 ctgggcatcc ccatggttat attagtgcaa tgtatcgagg agaattagcg attagcaaaa
     3121 tactatatgc taagaatgaa agcttatgcg aattaaaaaa acaggcagaa acttatccca
     3181 atgctttgca gaaaagttta gttaactttt ttatgtttga agcagggttc tctttaatgt
     3241 ttgtaaaagc aaattcggga acagacgata aatattatat tgcgggtcat gtttttcgta
     3301 tagtttcatg tttaaatcaa gtgttatttg catgtaataa tgcttattgt atcaacgaaa
     3361 agaaagctat aaaactgctt gaaacttttg aacataaacc tgaaaaatat accgagaagg
     3421 taaatcatat ttttgaagta ctcggtatct cactttttga atgctacgac atgaccgaga
     3481 agctttataa tgaagtgaat gaaattgtat cggagataaa taacttttta aacgaggaga
     3541 gttcagatga aagaaaacaa atatgatgat aatatatttt ttcaaaaata cagtcaaatg
     3601 agtcgctcgc agaaaggact ggctggtgcg ggagaatggg agactttgaa aaagatgcta
     3661 cctgatttta agggtaagcg tgtgcttgat ttaggatgcg gctatggatg gcactgtata
     3721 tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg atatttctca taaaatgctc
     3781 gaagtagcaa aaggaaaaac ccattttcca cagattgaat atgaatgctg tgccatagaa
     3841 gatgtggatt tcccagagga gagctttgat gtaatactaa gttcgcttgc gtttcattat
     3901 gtagcagact atgagaattt aataaaaaag atatatagga tgctgaaggc tggtggcaat
     3961 ttagttttta cagttgaaca tcctgttttt actgctcatg gaacacaaga ctggtattat
     4021 aacgaaaaag gagaaatact gcatttcccg gtggacaatt attattatga gggcaaacgg
     4081 acagctatgt ttttggaaga aaaggttaca aaatatcata gaacactgac cacatatcta
     4141 aatacactgc tttcaaatag ttttataata aatcagattg tggagccaca gccgccagag
     4201 aacatgatgg atattccggg gatggcggat gaaatgcgac gcccaatgat gctgattgta
     4261 tcggcaaaaa agaagatgta ataatataga aaaaataaac gaggagtatg taaatgagat
     4321 cagaaaaaga aatgatggat ttagtacttt ctttagcaga acaggatgaa cgtattcgaa
     4381 ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc taaagatgaa tttcaggatt
     4441 atgatattac atattttgta agtgatatag aacctagaac cgtttatatc taatgatgac
     4501 tggcttaatc aatttgggaa tataataatg atgcaaaagc cggaggatat ggaattattc
     4561 ccacctgaag aaaagggatt ttcctatctt atgctatttg atgattacaa taaaattgat
     4621 cttaccttat tgcccttgga agagttagat aattacctaa agggcgataa attaataaag
     4681 gttctaattg ataaagattg tagaattaaa agggacatag ttccgactga tatagattat
     4741 catgtaagaa agccaagcgc aagggagtat gatgattgct gcaatgaatt ttggaatgta
     4801 acaccttatg ttattaaagg attgtgccgt aaggaaattt tatttgctat tgatcatttt
     4861 aatcagattg ttcgccatga gctgctgaga atgatatcat ggaaggtcgg catcgaaaca
     4921 ggctttaaat taagtgtagg caagaactat aagtttattg aaaggtatat atccgaggat
     4981 ttgtgggaga aacttttgtc cacctaccgg atggattcct atgaaaacat atgggaagca
     5041 ttatttctat gccatcaatt gttcagggcg gtatccggtg aggtggcgga aaggcttcat
     5101 tatgcctatc cggagtatga taggaatata acaaaatata ccagggacat gtataaaaaa
     5161 tacactggta aaaccggctg cctggatagc acatatgccg ctgatataga agagaggcgg
     5221 gaacagtgat tacagaaatg aaagcagggc acctgaaaga tatcgataaa cccagcgaac
     5281 catttgaggt gataggtaag attataccga ggtatgaaaa cgagaattgg acctttacag
     5341 aattactcta tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat gaagaggatg
     5401 aggaggcaga ttgccttgaa tatattgaca atactgataa gataatatat ctttactacc
     5461 aagacgataa atgcgtcgga aaagttaaac tgcgaaaaaa ttggaaccgg tacgcttata
     5521 tagaagatat cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca
     5581 atatatctat agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg
     5641 acaataacct tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg
     5701 atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa
     5761 ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg
     5821 gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc
     5881 accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc
     5941 tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga
     6001 cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct
     6061 ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa
     6121 tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag
     6181 ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat
     6241 atcggattgt ccctatacga atagcttaga cagccg
//
LOCUS       DAYLZO010000367         6273 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.144, whole genome shotgun sequence.
ACCESSION   DAYLZO010000367 DAYLZO010000000
VERSION     DAYLZO010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.144"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            110..193
                     /locus_tag="ACGVJG_003370"
     CDS             110..193
                     /locus_tag="ACGVJG_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFJ6559227.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACGVJG_003371"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACGVJG_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFJ6559228.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1225..1485
                     /locus_tag="ACGVJG_003372"
                     /pseudo
     CDS             1225..1485
                     /locus_tag="ACGVJG_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1648..2313
                     /locus_tag="ACGVJG_003373"
                     /pseudo
     CDS             1648..2313
                     /locus_tag="ACGVJG_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2349..2594
                     /locus_tag="ACGVJG_003374"
     CDS             2349..2594
                     /locus_tag="ACGVJG_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFJ6559229.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2697..3566
                     /locus_tag="ACGVJG_003375"
     CDS             2697..3566
                     /locus_tag="ACGVJG_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFJ6559230.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3547..4281
                     /locus_tag="ACGVJG_003376"
     CDS             3547..4281
                     /locus_tag="ACGVJG_003376"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFJ6559231.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4314..5226
                     /gene="ant(6)"
                     /locus_tag="ACGVJG_003377"
                     /pseudo
     CDS             4314..5226
                     /gene="ant(6)"
                     /locus_tag="ACGVJG_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5223..5765
                     /gene="sat4"
                     /locus_tag="ACGVJG_003378"
     CDS             5223..5765
                     /gene="sat4"
                     /locus_tag="ACGVJG_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFJ6559232.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5858..>6273
                     /locus_tag="ACGVJG_003379"
     CDS             5858..>6273
                     /locus_tag="ACGVJG_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFJ6559233.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSR"
BASE COUNT         2224 a          938 c         1287 g         1824 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccat tttgtgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac tgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttac agatgtaata
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ttcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggagtatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttttaaattt
     1081 ggaaagttac acgttactaa agggaatgga gataaattat tagatatact actgacagct
     1141 tccaagaagc taaagaggtc cctagcgcct acggggaatt tgtatcgata aggggtacaa
     1201 attcccacta agcgctcggg accccttgta ggaaaatgtc ctaagtgtgg caacaatatt
     1261 gtattaaaaa aatcgtttta tggttgttca aattatcctg aatgtaagtt tactttagct
     1321 gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa aagaattact ggagggaaaa
     1381 gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc cctacaatgc cgttgtaaaa
     1441 attggggaaa agggatatat tgattttatc tctttttcaa aataaacatg gggctgaccc
     1501 aaaagtagct cctatatgaa aaatagaggt ctttccattt agatagtagt ggaaagacct
     1561 ctatttatct tttgaaagtg aatacaaaaa taaagcacac tctgctataa tataggtgat
     1621 taatccattt gagagaggtg ctttatcatg tatcaaaatt ataccataaa ccaactttgt
     1681 ttgcctatag atcttgaaat taaattagaa gaaaatgatt ttgctcatgc cattgtgcag
     1741 tttgttgact ctattcccga tgaagtcttt cttccttatt atcaatctat gggtagaccc
     1801 caatatcatc ctcgtatgct actttccatt attctttgtg cctatattca aggggtttat
     1861 tcgggccgcc aaatacaaaa tatgctgata gatagtattc ggatgcgcta cttatctcaa
     1921 gagcaattcc ctaactttcg tactattaat cgttttcgcg ttcatccgat tatgaacgat
     1981 attctagatc attcatttgt tcaatttcgt gagctactgg ttcaaagtgg cttaatcagt
     2041 ggcagtgctc tatacattga tggtacaaaa attgaagcgg atgcgaataa atactctttc
     2101 gtttggaaaa aaagtatttt aaagtataag gatagccttg atcaaaaagc ccttgaaaac
     2161 taccaacaaa tggtggaaac aaaaatctta cctgagttaa tagatgattt aaaggatgaa
     2221 ctttctatag aagagattga aaggattcgt caatctttgg aagaaaagga aaatcaatta
     2281 attgaagcga ttgaccaaac cgaaacagta gaagggggct gacccattat taataaggag
     2341 tggacaacat gattgatgta aatgagctat tggaagaagc tattagagaa actgaaaatc
     2401 taaatgatgg cgaagttttt cttgttaaag atttattcaa gggatatgtg gggaatagga
     2461 tacccagaaa ggatcgactt ctgcttggaa cgttattttt aaaccatgtt agcaaaatga
     2521 atggtaattt aaaggctatt gaaaagactt catctaatca gcagaggtat aaaaagacaa
     2581 ttgataagtt ttaactatct tttgtgaaat taaaaaaatt ataaatatgt tgagtagaag
     2641 caaaagattt ggcttggaat ttttacagca gtgaatcgat aaaaggagca tgcttgatgg
     2701 tagataatat tattaaatca gtagcagaga aattatcctc tctgtcttat atagaaggta
     2761 ttgttttagg tggttcacgt gcaaggggca cccatacaga ggattcggat atagatatcg
     2821 gcatctatta caattcagaa tcatttgaca taaatactat taatcaattc gctacaaagc
     2881 tggatgatga gcatagaaat aaccttgttg tacctcccgg agcatggggt gattggatta
     2941 atggcggcgg atggttagtc ataaacgggt atcatgtgga tttaatttta cgtgatatta
     3001 aacgtgtgga acaaataatg aaagatacag agcacggaat tgttactgcc aattatcaga
     3061 ctgggcatcc ccatggttat attagtgcaa tgtatcgagg agaattagcg attagcaaaa
     3121 tactatatgc taagaatgaa agcttatgcg aattaaaaaa acaggcagaa acttatccca
     3181 atgctttgca gaaaagttta gttaactttt ttatgtttga agcagggttc tctttaatgt
     3241 ttgtaaaagc aaattcggga acagacgata aatattatat tgcgggtcat gtttttcgta
     3301 tagtttcatg tttaaatcaa gtgttatttg catgtaataa tgcttattgt atcaacgaaa
     3361 agaaagctat aaaactgctt gaaacttttg aacataaacc tgaaaaatat accgagaagg
     3421 taaatcatat ttttgaagta ctcggtatct cactttttga atgctacgac atgaccgaga
     3481 agctttataa tgaagtgaat gaaattgtat cggagataaa taacttttta aacgaggaga
     3541 gttcagatga aagaaaacaa atatgatgat aatatatttt ttcaaaaata cagtcaaatg
     3601 agtcgctcgc agaaaggact ggctggtgcg ggagaatggg agactttgaa aaagatgcta
     3661 cctgatttta agggtaagcg tgtgcttgat ttaggatgcg gctatggatg gcactgtata
     3721 tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg atatttctca taaaatgctc
     3781 gaagtagcaa aaggaaaaac ccattttcca cagattgaat atgaatgctg tgccatagaa
     3841 gatgtggatt tcccagagga gagctttgat gtaatactaa gttcgcttgc gtttcattat
     3901 gtagcagact atgagaattt aataaaaaag atatatagga tgctgaaggc tggtggcaat
     3961 ttagttttta cagttgaaca tcctgttttt actgctcatg gaacacaaga ctggtattat
     4021 aacgaaaaag gagaaatact gcatttcccg gtggacaatt attattatga gggcaaacgg
     4081 acagctatgt ttttggaaga aaaggttaca aaatatcata gaacactgac cacatatcta
     4141 aatacactgc tttcaaatag ttttataata aatcagattg tggagccaca gccgccagag
     4201 aacatgatgg atattccggg gatggcggat gaaatgcgac gcccaatgat gctgattgta
     4261 tcggcaaaaa agaagatgta ataatataga aaaaataaac gaggagtatg taaatgagat
     4321 cagaaaaaga aatgatggat ttagtacttt ctttagcaga acaggatgaa cgaacgtatt
     4381 cgaattgtga cccttgaggg gtcacgcgca aatattaata tacctaaaga tgaatttcag
     4441 gattatgata ttacatattt tgtaagtgat atagaaccgt ttatatctaa tgatgactgg
     4501 cttaatcaat ttgggaatat aataatgatg caaaagccgg aggatatgga attattccca
     4561 cctgaagaaa agggattttc ctatcttatg ctatttgatg attacaataa aattgatctt
     4621 accttattgc ccttggaaga gttagataat tacctaaagg gcgataaatt aataaaggtt
     4681 ctaattgata aagattgtag aattaaaagg gacatagttc cgactgatat agattatcat
     4741 gtaagaaagc caagcgcaag ggagtatgat gattgctgca atgaattttg gaatgtaaca
     4801 ccttatgtta ttaaaggatt gtgccgtaag gaaattttat ttgctattga tcattttaat
     4861 cagattgttc gccatgagct gctgagaatg atatcatgga aggtcggcat cgaaacaggc
     4921 tttaaattaa gtgtaggcaa gaactataag tttattgaaa ggtatatatc cgaggatttg
     4981 tgggagaaac ttttgtccac ctaccggatg gattcctatg aaaacatatg ggaagcatta
     5041 tttctatgcc atcaattgtt cagggcggta tccggtgagg tggcggaaag gcttcattat
     5101 gcctatccgg agtatgatag gaatataaca aaatatacca gggacatgta taaaaaatac
     5161 actggtaaaa ccggctgcct ggatagcaca tatgccgctg atatagaaga gaggcgggaa
     5221 cagtgattac agaaatgaaa gcagggcacc tgaaagatat cgataaaccc agcgaaccat
     5281 ttgaggtgat aggtaagatt ataccgaggt atgaaaacga gaattggacc tttacagaat
     5341 tactctatga agcgccatat ttaaaaagct accaagacga agaggatgaa gaggatgagg
     5401 aggcagattg ccttgaatat attgacaata ctgataagat aatatatctt tactaccaag
     5461 acgataaatg cgtcggaaaa gttaaactgc gaaaaaattg gaaccggtac gcttatatag
     5521 aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg cttatcaata
     5581 tatctataga atgggcaaag cataaaaact tgcatggact aatgcttgaa acccaggaca
     5641 ataaccttat agcttgtaaa ttctatcata attgtggttt caaaatcggc tccgtcgata
     5701 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
     5761 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
     5821 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
     5881 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
     5941 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
     6001 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
     6061 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
     6121 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
     6181 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
     6241 ggattgtccc tatacgaata gcttagacag ccg
//
LOCUS       DAYLZO010000368         4995 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.165, whole genome shotgun sequence.
ACCESSION   DAYLZO010000368 DAYLZO010000000
VERSION     DAYLZO010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.165"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(30..2180)
                     /locus_tag="ACGVJG_003380"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="ACGVJG_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="ACGVJG_003381"
     CDS             2326..2901
                     /locus_tag="ACGVJG_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFJ6559234.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR-A"
                     /locus_tag="ACGVJG_003382"
     CDS             3115..3810
                     /gene="vanR-A"
                     /locus_tag="ACGVJG_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFJ6559235.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="ACGVJG_003383"
     CDS             3788..4942
                     /locus_tag="ACGVJG_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFJ6559236.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DAYLZO010000369         3355 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.199, whole genome shotgun sequence.
ACCESSION   DAYLZO010000369 DAYLZO010000000
VERSION     DAYLZO010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.199"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            314..811
                     /gene="dfrG"
                     /locus_tag="ACGVJG_003384"
     CDS             314..811
                     /gene="dfrG"
                     /locus_tag="ACGVJG_003384"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFJ6559237.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(883..2835)
                     /locus_tag="ACGVJG_003385"
     CDS             complement(883..2835)
                     /locus_tag="ACGVJG_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFJ6559238.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2842..3078)
                     /locus_tag="ACGVJG_003386"
     CDS             complement(2842..3078)
                     /locus_tag="ACGVJG_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559239.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1041 a          582 c          547 g         1185 t
ORIGIN      
        1 gtaaacctaa attagtaaag aagcaacagt aatctatact aagtagttca gttttggagt
       61 acaaaatgac aaaatgtact tcaaaatgga ctactttttt cattttaact tacataccaa
      121 attacataca aaatgattta taaaatgcaa ttgaaaatga taattgaatg aaaatttgtg
      181 attttgtggt attatgataa tataacttat agaaaaaatt gcttattcaa ctaacgggca
      241 ggataatgag aaaaggtttt ttgatagaaa taagaaatta gatggtaaat tagttaaaaa
      301 ggctgtgtta taaatgaaag tttctttgat tgctgcgatg gataagaata gagtgattgg
      361 caaagagaat gacattcctt ggaggattcc caaggactgg gaatatgtta aaaatactac
      421 aaagggacat ccgataatat taggtaggaa gaaccttgaa tcaatcggaa gagccttacc
      481 tgacagaaga aatattattc tgacgagaga taaggggttt acctttaatg gttgtgaaat
      541 tgttcattca atagaagatg tttttgagtt atgtaaaaac gaagaagaaa tttttatttt
      601 cggaggagaa cagatttata atttgttttt cccttatgtt gagaaaatgt acatcacaaa
      661 aatacatcat gaattcgaag gagatacttt ttttccagaa gtgaattatg aggaatggaa
      721 tgaggtattt gcccaaaaag ggataaagaa tgataaaaat ccgtataact actattttca
      781 tgtatatgaa agaaaaaact tattgagtta acgggtgcgt taattgaaga agaacagtaa
      841 agataaagca caagagcttg ggattactcc aagctctttt tgtcatttga aataaattat
      901 atctttccaa tcaaattgtt tatctgtaat ccctaatctt tgagcaggtg tcaaaacctt
      961 tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa gttcttagaa ttgtaatcgc
     1021 aaattgagca tacttcggat tgaagttagc gtaaatatag ctcttcttat caccacgagc
     1081 tgttgttaat ggtctttcaa gagatgatat ataacgtctt atctgttgaa taaagctatt
     1141 ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt tcgtttggtt ccagagcaga
     1201 taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc ttagtggcta atggatgttt
     1261 gatagggtta tcggcatatt tgcgatatct ttctccatct ttactaatag cctcttcgtg
     1321 gaattgatga cttgttagta attcagttag ataatgagat gccaaagtcc gtagaaactt
     1381 tgttttgaat cctctgatat ctccccaatc gagcagttct gcttttgctt gtccgaattc
     1441 ttttaagcat tgttttctgc ttttcgtctt gttaacttgg caaataaaat gatgtgcatc
     1501 gctcaacctt aattctttcg tgaatactct ataaaaggcg tttctaatag agaagtcatc
     1561 atcactaatc attcgccatt cgtcggcatt aaccatttgt ttaattaacc aatagtgagc
     1621 agttgtagta taaggagcat caacatttaa accatcgaca aatctactcc tattagtaat
     1681 tttccctaat tcgtgaagat aagcattcct attttcgcta tcgttagcgg atggttcttg
     1741 tggataataa gaccaatcta acctatcatt tttacgacta aaagtattga gatggtcttc
     1801 tttgaatttt cttgtatctt catttaattc atccattgaa atattccaat cataggcaac
     1861 atcagaacgg aacacatatc ttgataagac ctcagcagat acaactatat acgtttgcat
     1921 attaaggtct tcaaaccccg tatatttctt tgaaccttgt cctttcttac ggacgttatt
     1981 taggtagtag tgcatcttgt ccgtattaag ccacatctcg ttaaatttct ttgtttgaag
     2041 gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc ctatacagcc actccagctt
     2101 atgatagtag gttccaactc caattcctaa aatatcacaa gtacgactga ctgaaaccct
     2161 tccaacaacc atcttagcga acattggaag aatggtgttc ttttgttggt gataagtagt
     2221 ggtttcttct ctttttggta gaacattagt gaacttctta cacgccttac attgatagcg
     2281 ttgagatttg cccctacttt tccccctttt gtaaaattgc ttcggctcat tgaatggggt
     2341 tgattcttct tccgagcagc cttctttatg aaattgataa tctggctcaa catcttggat
     2401 gctattgatt tctataaggc gtttaatttc ttcaacaaca gaccaattag ataaaggggt
     2461 tactgtacag ttttgcgata cacctctgcc aataggattg tcattgcagt acataccttt
     2521 atgaccttta tctttggaac taccagtcat tttatatcga cttggtttcc cttttacatc
     2581 tttgaatttg tgctgttctt tcccaaaatt acaacaaaac ggattgatgc agtaattata
     2641 ttgaatggga tattcagaac cgttccattt aaaagacgtt ggcaagaaca acattttgtt
     2701 ataccttgtt gcaaatcgtt ttggagttaa taaatcatat tgtttagccc tatcttttaa
     2761 ttcttcgttt gaaatcggaa ttttaacatc tacaatttca tcttccttcg tggctaatct
     2821 tttaagcttg gtcattgtct atcaacccac tctatctttg atacccattt tttcaacaat
     2881 ccgcttatta cttcgcttgt tggcaatcca ttcaataaac ttcttatcgt ctaaaatgtt
     2941 caataagaat tcatccactg cttcgctttc agtacgctct gcatattcag cgtattgctt
     3001 aactatcacc cttacttgct ctgatatttc ccaatccaca gacttagcgt tcttattttt
     3061 tggctcaaga aattgcatag gaaaatgcac ccctttttgc ttagtaaatt gtaaccattt
     3121 taacacacca atttgcattc ctacaataat tatttcacgt tcgcattttt atatacattt
     3181 taacttacat tttgtggtgt aaattaacaa caaacgttga tttaacaata aaaaacgagt
     3241 atcaaattca gttgcaattt gatactcatt taggtttaca ttttcacttt ttgtactcca
     3301 aaactgaact acttaaatct atactgctgc ctcttttttt tagtacatgt ccttc
//
LOCUS       DAYLZO010000370         3121 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.208, whole genome shotgun sequence.
ACCESSION   DAYLZO010000370 DAYLZO010000000
VERSION     DAYLZO010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.208"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            364..1842
                     /gene="msr(C)"
                     /locus_tag="ACGVJG_003387"
     CDS             364..1842
                     /gene="msr(C)"
                     /locus_tag="ACGVJG_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFJ6559240.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1889..2335
                     /locus_tag="ACGVJG_003388"
     CDS             1889..2335
                     /locus_tag="ACGVJG_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFJ6559241.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2534..3049)
                     /locus_tag="ACGVJG_003389"
     CDS             complement(2534..3049)
                     /locus_tag="ACGVJG_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFJ6559242.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT          989 a          528 c          643 g          961 t
ORIGIN      
        1 gttcgaataa aaggttttca gttactattc agtagctatt gctttttttg aatcgtgtgt
       61 ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa
      121 cttttgaata acaactaaat aaattcaaaa gacatgtata aacaacaatt tcatggatgg
      181 agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa gctatgacag atttggtgat
      241 ttagtcatag ttatttcgtt cagagtagga aaagatgtga tgcagttttt ttgcaaacag
      301 atttttctgc tcttttttag tatcgataat tgttgtttta catgaaatag gagtgtaaaa
      361 catatggaaa atttagcagt aaatataaca aatctacaag tcagctttgg caaccagcta
      421 gaactatcta ttgattctct tcgtgtctat cagcaagatc gtatagggat cataggagaa
      481 aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt tcccgatcat
      541 gggaaaatcc aaacagagat caccttcaac tacctgcctc aattaaccta tctcgctgaa
      601 gcaaaggacc taaatttgga attagccagt catttccagt taagactgga agaaacttca
      661 gagcggaaat ggagcggagg ggaagaacga aagatcgagt tgatacgtct tctttcttct
      721 tatgaacaag ggatgcttct agacgagcca acaacccatc tagatagaaa aagtattgat
      781 cgactgattg aagagcttcg ttattattat ggtacgctgg tttttgttag tcatgatcgc
      841 tattttctag atgagctagc atcgaaaatc tgggaagtaa aagacggaga aatccgagag
      901 ttttcgggga attatagtgc ctatctcact caaaaggaat tggagaaaaa gactcagcta
      961 cgagaagcag agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag
     1021 aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga
     1081 ccggatcggt tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat
     1141 gcgaaaacat tagagagaag actccaaaaa ataggagaaa caaccaagcc gcagcagatg
     1201 aaacaaatcc gttttccagt accaaaatct cttgaactcc acagccgcta tcccatcatg
     1261 ggacaaaatg tccaattgga aagaagtggg agaacattac tggtaaatgg tgattttcag
     1321 ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta
     1381 ttagaacata tccgcaaaca aggagaagga atccttctct ctccgaaagt aagctttcaa
     1441 gtatatcagc aaaagggtta tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg
     1501 agacaaacag agttttcaga atcgcttgtc cgtagtttgc tgaatcactt agggtttgct
     1561 caggaaactt tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg
     1621 attgctttgc tttttactaa gccaagtaat gtgttgctgt tagatgaacc gactaatttt
     1681 attgatatgg caacgatcga agctttagag aagctaatgc aaatatatcc gggaacgatt
     1741 ttgtttactt ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata
     1801 aaagggcaga aaataaaaaa agtacttacg agaaattttt aaatgtgcta cgatgatttt
     1861 cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag gaataatttt
     1921 tacaacaatg atgtgttttt tgatggtctt ggggatgagc gcgtataatt tatggctgca
     1981 tcaagatctt catttatcaa gtttgttgat tggtctagtg cctggattcg tggtggcttt
     2041 cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta aactaccagt
     2101 aaatccagaa agcaaggtcc aaatgatctt aactatttca tgcttgatgg ttttaggaat
     2161 ggtgacatgt atgtctttat tcggtgtgat catggaacaa ggaatacctg atcaattatt
     2221 ctctgcttat atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt tgcagttgat
     2281 catcgttggc ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg aatgatggaa
     2341 cactgttatt tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg aatgcagttc
     2401 tagaaaaata gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc tttttctttt
     2461 ccaactgtat tgctctttta ttgaagataa tttgctcttc aatagatatt tctagaaagg
     2521 ttctgtttat cccttactca aaaaactgct gcatttgttc tcttacttgc tgacaccatt
     2581 ggagttcctg ctgacaaagc ataattctgc gctggatcac aacataacgt ccaaattctt
     2641 gtttcttgtc tagcgagagg tcattcaatt ttttttgatt tttttgcatg attttgttca
     2701 gctgttgttc tctagtgaat agcagtccag tagctgttgt tttatcgagc gtcgaaaaag
     2761 catagatttt tgctagaaat tcatcttttt gcgtaggagc gggagtttcc tctttgatcc
     2821 attcctctac aagagcacgt ccttcatcag tcaagtggta gatctttttt tgagagtgct
     2881 cgtctttgtc caaaacggtc acataaccct gctggtctaa cttggcaagc gttgtataga
     2941 tctgactgtg atgggcttcc caaaaaattg tgatcagctg cttcaattca tacccagaac
     3001 gtggtttgga taaaagcacg cataataata tataggctaa agtattcata aaaatcctac
     3061 ctctcgtctt attttcttta ttatatcaca gtaaagtttc cacgttttac atgtgcatat
     3121 t
//
LOCUS       DAYLZO010000371         3121 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.209, whole genome shotgun sequence.
ACCESSION   DAYLZO010000371 DAYLZO010000000
VERSION     DAYLZO010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.209"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            364..1842
                     /gene="msr(C)"
                     /locus_tag="ACGVJG_003390"
     CDS             364..1842
                     /gene="msr(C)"
                     /locus_tag="ACGVJG_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFJ6559243.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1889..2335
                     /locus_tag="ACGVJG_003391"
     CDS             1889..2335
                     /locus_tag="ACGVJG_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFJ6559244.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2534..3049)
                     /locus_tag="ACGVJG_003392"
     CDS             complement(2534..3049)
                     /locus_tag="ACGVJG_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFJ6559245.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT          989 a          527 c          643 g          962 t
ORIGIN      
        1 gttcgaataa aaggttttca gttactattc agtagctatt gctttttttg aatcgtgtgt
       61 ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa
      121 cttttgaata acaactaaat aaattcaaaa gacatgtata aacaacaatt tcatggatgg
      181 agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa gctatgacag atttggtgat
      241 ttagtcatag ttatttcgtt cagagtagga aaagatgtga tgcagttttt ttgcaaacag
      301 atttttctgc tcttttttag tatcgataat tgttgtttta catgaaatag gagtgtaaaa
      361 catatggaaa atttagcagt aaatataaca aatctacaag tcagctttgg caaccagcta
      421 gaactatcta ttgattctct tcgtgtctat cagcaagatc gtatagggat cataggagaa
      481 aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt tcccgatcat
      541 gggaaaatcc aaacagagat caccttcaac tacctgcctc aattaaccta tctcgctgaa
      601 gcaaaggacc taaatttgga attagccagt catttccagt taagactgga agaaacttca
      661 gagcggaaat ggagcggagg ggaagaacga aagatcgagt tgatacgtct tctttcttct
      721 tatgaacaag ggatgcttct agacgagcca acaacccatc tagatagaaa aagtattgat
      781 cgactgattg aagagcttcg ttattattat ggtacgctgg tttttgttag tcatgatcgc
      841 tattttctag atgagctagc atcgaaaatc tgggaagtaa aagacggaga aatccgagag
      901 ttttcgggga attatagtgc ctatctcact caaaaggaat tggagaaaaa gactcagcta
      961 cgagaagcag agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag
     1021 aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga
     1081 ctggatcggt tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat
     1141 gcgaaaacat tagagagaag actccaaaaa ataggagaaa caaccaagcc gcagcagatg
     1201 aaacaaatcc gttttccagt accaaaatct cttgaactcc acagccgcta tcccatcatg
     1261 ggacaaaatg tccaattgga aagaagtggg agaacattac tggtaaatgg tgattttcag
     1321 ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta
     1381 ttagaacata tccgcaaaca aggagaagga atccttctct ctccgaaagt aagctttcaa
     1441 gtatatcagc aaaagggtta tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg
     1501 agacaaacag agttttcaga atcgcttgtc cgtagtttgc tgaatcactt agggtttgct
     1561 caggaaactt tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg
     1621 attgctttgc tttttactaa gccaagtaat gtgttgctgt tagatgaacc gactaatttt
     1681 attgatatgg caacgatcga agctttagag aagctaatgc aaatatatcc gggaacgatt
     1741 ttgtttactt ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata
     1801 aaagggcaga aaataaaaaa agtacttacg agaaattttt aaatgtgcta cgatgatttt
     1861 cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag gaataatttt
     1921 tacaacaatg atgtgttttt tgatggtctt ggggatgagc gcgtataatt tatggctgca
     1981 tcaagatctt catttatcaa gtttgttgat tggtctagtg cctggattcg tggtggcttt
     2041 cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta aactaccagt
     2101 aaatccagaa agcaaggtcc aaatgatctt aactatttca tgcttgatgg ttttaggaat
     2161 ggtgacatgt atgtctttat tcggtgtgat catggaacaa ggaatacctg atcaattatt
     2221 ctctgcttat atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt tgcagttgat
     2281 catcgttggc ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg aatgatggaa
     2341 cactgttatt tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg aatgcagttc
     2401 tagaaaaata gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc tttttctttt
     2461 ccaactgtat tgctctttta ttgaagataa tttgctcttc aatagatatt tctagaaagg
     2521 ttctgtttat cccttactca aaaaactgct gcatttgttc tcttacttgc tgacaccatt
     2581 ggagttcctg ctgacaaagc ataattctgc gctggatcac aacataacgt ccaaattctt
     2641 gtttcttgtc tagcgagagg tcattcaatt ttttttgatt tttttgcatg attttgttca
     2701 gctgttgttc tctagtgaat agcagtccag tagctgttgt tttatcgagc gtcgaaaaag
     2761 catagatttt tgctagaaat tcatcttttt gcgtaggagc gggagtttcc tctttgatcc
     2821 attcctctac aagagcacgt ccttcatcag tcaagtggta gatctttttt tgagagtgct
     2881 cgtctttgtc caaaacggtc acataaccct gctggtctaa cttggcaagc gttgtataga
     2941 tctgactgtg atgggcttcc caaaaaattg tgatcagctg cttcaattca tacccagaac
     3001 gtggtttgga taaaagcacg cataataata tataggctaa agtattcata aaaatcctac
     3061 ctctcgtctt attttcttta ttatatcaca gtaaagtttc cacgttttac atgtgcatat
     3121 t
//
LOCUS       DAYLZO010000372         2824 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.217, whole genome shotgun sequence.
ACCESSION   DAYLZO010000372 DAYLZO010000000
VERSION     DAYLZO010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.217"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            119..1087
                     /locus_tag="ACGVJG_003393"
     CDS             119..1087
                     /locus_tag="ACGVJG_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFJ6559246.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1080..2111
                     /gene="vanA"
                     /locus_tag="ACGVJG_003394"
     CDS             1080..2111
                     /gene="vanA"
                     /locus_tag="ACGVJG_003394"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFJ6559247.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2117..2725
                     /locus_tag="ACGVJG_003395"
     CDS             2117..2725
                     /locus_tag="ACGVJG_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFJ6559248.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          831 a          547 c          696 g          750 t
ORIGIN      
        1 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
       61 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
      121 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
      181 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
      241 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
      301 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
      361 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
      421 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
      481 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
      541 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
      601 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
      661 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
      721 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
      781 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
      841 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
      901 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
      961 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     1021 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     1081 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     1141 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     1201 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     1261 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     1321 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     1381 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     1441 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     1501 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     1561 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     1621 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     1681 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     1741 gaaacagtgc cgcgttagct gttggcgagg tggaccaaat caggctgcag tacggaatct
     1801 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     1861 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     1921 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     1981 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     2041 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     2101 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     2161 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     2221 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     2281 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaatcgtgc
     2341 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     2401 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     2461 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     2521 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     2581 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     2641 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     2701 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct
     2761 aactctttcg gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta
     2821 gata
//
LOCUS       DAYLZO010000373         2772 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.223, whole genome shotgun sequence.
ACCESSION   DAYLZO010000373 DAYLZO010000000
VERSION     DAYLZO010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.223"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            460..955
                     /gene="dfrF"
                     /locus_tag="ACGVJG_003396"
                     /pseudo
     CDS             460..955
                     /gene="dfrF"
                     /locus_tag="ACGVJG_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
     gene            1060..1716
                     /locus_tag="ACGVJG_003397"
     CDS             1060..1716
                     /locus_tag="ACGVJG_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFJ6559249.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            1898..2428
                     /locus_tag="ACGVJG_003398"
     CDS             1898..2428
                     /locus_tag="ACGVJG_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFJ6559250.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1007 a          360 c          585 g          820 t
ORIGIN      
        1 gcgcagcaga gatggcaacg gtgaaaggat gggaaaaagg tgtttcccaa acaaaaagat
       61 attaagaaac agcccatgtt tacctgcttg caaacaaaag aatattgcta ctagactaag
      121 agatactaca taagtgtgtt ggtatctctt ttttgttgcc gtgaaacagc agactatttc
      181 atttctgctt gattacctta aaacttatta gtctatggtt tgtcaagggc ttgttgcgtg
      241 agaaatgctg aatatcaccc tttacaaaac ataggctgat aagtaacttt tcaaaagcag
      301 aaaagaaatg gcaatgctta taacaatatg atataatgca gatgaagaca aaaaaggaat
      361 ttgtcgagct ggttagttcg agataatttt gagtttgtta agatgtggtg cacttgatgg
      421 aatcacaggt acgacatcat attatggagg atgaaataga tgataggttt gattgttgcg
      481 aggtcaaaga ataatgttat aggcaagaat ggtaatatac catggaaaat aaagggagaa
      541 caaaagcaat ttagagagtt aacaacgggt aatgtggtta ttatggggcg aaagtcttat
      601 gaagaaatcg gtcatccgtt gcctaataga atgaatattg ttgtttccac cacaacagag
      661 tatcaaggag ataatttagt ttcagttaaa tcattagaag atgcattagt attggctaaa
      721 ggacgagatg tatacatatc tggtggatat ggactattta aggaagcttt gcaaatagta
      781 gataaaatgt atatcacaga agtagattta aatattgaag atggagatac attctttcca
      841 gaatttgata tcaatgattt tgaagttttg ataggggaaa cacttggtga ggaagtgaaa
      901 tatacgagaa cattttatgt aaggaaaaaa tgaattgagt aaattttgga tttagggtgt
      961 tttcataaat atattgcttt ttatgcatat ataaattgtt gtaagacaaa ttccagtttg
     1021 tatggggcga taaaatcaga atttgagaga ggattgaata tgaataagga atggtctgaa
     1081 cttaataaaa caatgcaggc acaaataaaa aagaaagata cttataagag gggtattgat
     1141 actttactta ctttacgaag tcagttaata caaaccttag tatctttcaa agaagaatta
     1201 tgcagagaag attttaactc aatccccttc ataaacgctg atggttatca tagtaagacg
     1261 attgcttatt ctatttggca tattttccgc attgaagata tagttgtgca tacagtgata
     1321 aatgaagatg aacaagtatt ttttgcaggc aattatcaag agcgtatcaa ttcacctatc
     1381 attacaacag gaaatgaact catgaaacag cagattgcag acttttcaaa acagcttaat
     1441 ctggaagaat tatatttata tattttcgaa gtatgggaaa gcactgagaa aatgcttgaa
     1501 cggttatctt atgatgaatt gaaaagaaaa ataccgaaag aaagaaaagg atacttagaa
     1561 tcgttgaatg tagtaaacga caatgaaaaa gcaatttggc tgattgatta ttggtgtaat
     1621 aaagatattt gtggactaat tcaaatgccg ttttcaagac attggattat gcacacagaa
     1681 gcctgtttgc gtataaagaa caagatacat tcataggcaa aaatttcagt ttgtagaact
     1741 aaggaaattg gaatttaagg aggaataaag tatggttagt gattttataa gagcagcatt
     1801 tccatggatt ttaatgggtt tatttgtagc aatcagttgt tcccttatga gtaaaaaaga
     1861 gaaataaatt cctgtttgta tttataaaag agaaaaaatg agaaaagtag ttttatttat
     1921 tgccatgagc cttgacggat atattgcgga cggtaatggt ggagtagctt ggctaaatgg
     1981 tcatggaaat gacaatgaaa acatcgacac ttatacagaa tttaccaaag atatagatac
     2041 tgtcttaatg gggtggaata cttctcatca ggttgttact gaactttctc cgcaagaatg
     2101 ggtatataat aaatttacaa cttatgtatt aacacataaa gagtgcaatt cttcccaaca
     2161 aattcatttt acaagtgaaa atcctgtttt attattggaa cggctaaaac aagaagcagg
     2221 aaaggatatt tggatttgtg gcggagcaaa tcttgttcag cagttggtaa gcaaaaatat
     2281 gattgataaa tattacattt ctgttattcc tactctgtta ggaaatgggg ttcgtctttt
     2341 gggcaacata gacagggaaa taaagttaag gctttgtaaa acacagactt ataacggaat
     2401 aaccgatttg atttatatgc gtagataact agagagaaac agaaaagctg ttaaaccgac
     2461 ggagcgatcc gaccacgttg gcgggaaaat ccttaagatt tttccgccat gcttgcaagg
     2521 gggcggttcg cttcggcggt tcggcgagct tgcgagccgt tcaaagcccc cgttatctaa
     2581 caaggggggc acaaaaaaca agaaacacta tacactaaca agcggaaaac gctgtattta
     2641 taaggcaaaa ccccattttt acagtagtga tataaattgt acctatatca ctatgaaaat
     2701 tgaatagtaa atatgagaaa gacagttgat ttcaaaaaat aggctctttg tcaaatgatg
     2761 ttggtaaaga ga
//
LOCUS       DAYLZO010000374         2772 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.224, whole genome shotgun sequence.
ACCESSION   DAYLZO010000374 DAYLZO010000000
VERSION     DAYLZO010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.224"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            460..955
                     /gene="dfrF"
                     /locus_tag="ACGVJG_003399"
                     /pseudo
     CDS             460..955
                     /gene="dfrF"
                     /locus_tag="ACGVJG_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
     gene            1060..1716
                     /locus_tag="ACGVJG_003400"
     CDS             1060..1716
                     /locus_tag="ACGVJG_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFJ6559251.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            1898..2428
                     /locus_tag="ACGVJG_003401"
     CDS             1898..2428
                     /locus_tag="ACGVJG_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFJ6559252.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1007 a          360 c          584 g          821 t
ORIGIN      
        1 gcgcagcaga gatggcaacg gtgaaaggat gggaaaaagg tgtttcccaa acaaaaagat
       61 attaagaaac agcccatgtt tacctgcttg caaacaaaag aatattgcta ctagactaag
      121 agatactaca taagtgtgtt ggtatctctt ttttgttgcc gtgaaacagc agactatttc
      181 atttctgctt gattacctta aaacttatta gtctatggtt tgtcaagggc ttgttgcgtg
      241 agaaatgctg aatatcaccc tttacaaaac ataggctgat aagtaacttt tcaaaagcag
      301 aaaagaaatg gcaatgctta taacaatatg atataatgca gatgaagaca aaaaaggaat
      361 ttgtcgagct ggttagttcg agataatttt gagtttgtta agatgtggtg cacttgatgg
      421 aatcacaggt acgacatcat attatggagg atgaaataga tgataggttt gattgttgcg
      481 aggtcaaaga ataatgttat aggcaagaat ggtaatatac catggaaaat aaagggagaa
      541 caaaagcaat ttagagagtt aacaacgggt aatgtggtta ttatggggcg aaagtcttat
      601 gaagaaatcg gtcatccgtt gcctaataga atgaatattg ttgtttccac cacaacagag
      661 tatcaaggag ataatttagt ttcagttaaa tcattagaag atgcattatt attggctaaa
      721 ggacgagatg tatacatatc tggtggatat ggactattta aggaagcttt gcaaatagta
      781 gataaaatgt atatcacaga agtagattta aatattgaag atggagatac attctttcca
      841 gaatttgata tcaatgattt tgaagttttg ataggggaaa cacttggtga ggaagtgaaa
      901 tatacgagaa cattttatgt aaggaaaaaa tgaattgagt aaattttgga tttagggtgt
      961 tttcataaat atattgcttt ttatgcatat ataaattgtt gtaagacaaa ttccagtttg
     1021 tatggggcga taaaatcaga atttgagaga ggattgaata tgaataagga atggtctgaa
     1081 cttaataaaa caatgcaggc acaaataaaa aagaaagata cttataagag gggtattgat
     1141 actttactta ctttacgaag tcagttaata caaaccttag tatctttcaa agaagaatta
     1201 tgcagagaag attttaactc aatccccttc ataaacgctg atggttatca tagtaagacg
     1261 attgcttatt ctatttggca tattttccgc attgaagata tagttgtgca tacagtgata
     1321 aatgaagatg aacaagtatt ttttgcaggc aattatcaag agcgtatcaa ttcacctatc
     1381 attacaacag gaaatgaact catgaaacag cagattgcag acttttcaaa acagcttaat
     1441 ctggaagaat tatatttata tattttcgaa gtatgggaaa gcactgagaa aatgcttgaa
     1501 cggttatctt atgatgaatt gaaaagaaaa ataccgaaag aaagaaaagg atacttagaa
     1561 tcgttgaatg tagtaaacga caatgaaaaa gcaatttggc tgattgatta ttggtgtaat
     1621 aaagatattt gtggactaat tcaaatgccg ttttcaagac attggattat gcacacagaa
     1681 gcctgtttgc gtataaagaa caagatacat tcataggcaa aaatttcagt ttgtagaact
     1741 aaggaaattg gaatttaagg aggaataaag tatggttagt gattttataa gagcagcatt
     1801 tccatggatt ttaatgggtt tatttgtagc aatcagttgt tcccttatga gtaaaaaaga
     1861 gaaataaatt cctgtttgta tttataaaag agaaaaaatg agaaaagtag ttttatttat
     1921 tgccatgagc cttgacggat atattgcgga cggtaatggt ggagtagctt ggctaaatgg
     1981 tcatggaaat gacaatgaaa acatcgacac ttatacagaa tttaccaaag atatagatac
     2041 tgtcttaatg gggtggaata cttctcatca ggttgttact gaactttctc cgcaagaatg
     2101 ggtatataat aaatttacaa cttatgtatt aacacataaa gagtgcaatt cttcccaaca
     2161 aattcatttt acaagtgaaa atcctgtttt attattggaa cggctaaaac aagaagcagg
     2221 aaaggatatt tggatttgtg gcggagcaaa tcttgttcag cagttggtaa gcaaaaatat
     2281 gattgataaa tattacattt ctgttattcc tactctgtta ggaaatgggg ttcgtctttt
     2341 gggcaacata gacagggaaa taaagttaag gctttgtaaa acacagactt ataacggaat
     2401 aaccgatttg atttatatgc gtagataact agagagaaac agaaaagctg ttaaaccgac
     2461 ggagcgatcc gaccacgttg gcgggaaaat ccttaagatt tttccgccat gcttgcaagg
     2521 gggcggttcg cttcggcggt tcggcgagct tgcgagccgt tcaaagcccc cgttatctaa
     2581 caaggggggc acaaaaaaca agaaacacta tacactaaca agcggaaaac gctgtattta
     2641 taaggcaaaa ccccattttt acagtagtga tataaattgt acctatatca ctatgaaaat
     2701 tgaatagtaa atatgagaaa gacagttgat ttcaaaaaat aggctctttg tcaaatgatg
     2761 ttggtaaaga ga
//
LOCUS       DAYLZO010000375         2244 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.243, whole genome shotgun sequence.
ACCESSION   DAYLZO010000375 DAYLZO010000000
VERSION     DAYLZO010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.243"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..755
                     /locus_tag="ACGVJG_003402"
     CDS             <1..755
                     /locus_tag="ACGVJG_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFJ6559253.1"
                     /translation="EPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDY
                     NKIDLTLLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCC
                     NEFWNVTPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKF
                     IERYISEDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNI
                     TKYTRDMYKKYTGKTGCLDSTYAADIEERREQ"
     gene            752..1294
                     /gene="sat4"
                     /locus_tag="ACGVJG_003403"
     CDS             752..1294
                     /gene="sat4"
                     /locus_tag="ACGVJG_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFJ6559254.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1387..2181
                     /locus_tag="ACGVJG_003404"
                     /note="aph(3')-IIIa"
     CDS             1387..2181
                     /locus_tag="ACGVJG_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFJ6559255.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          750 a          354 c          532 g          608 t
ORIGIN      
        1 tagaaccgtt tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc
       61 aaaagccgga ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc
      121 tatttgatga ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt
      181 acctaaaggg cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg
      241 acatagttcc gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg
      301 attgctgcaa tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg
      361 aaattttatt tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga
      421 tatcatggaa ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt
      481 ttattgaaag gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg
      541 attcctatga aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat
      601 ccggtgaggt ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa
      661 aatataccag ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat
      721 atgccgctga tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct
      781 gaaagatatc gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta
      841 tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
      901 ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac
      961 tgataagata atatatcttt actaccaaga cgataaatgc gtcggaaaag ttaaactgcg
     1021 aaaaaattgg aaccggtacg cttatataga agatatcgcc gtatgtaagg atttcagggg
     1081 gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt
     1141 gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa
     1201 ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     1261 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt
     1321 cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa
     1381 taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc
     1441 tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat
     1501 gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa
     1561 cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac
     1621 tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc
     1681 tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc
     1741 atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc
     1801 cgattagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac
     1861 tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa
     1921 aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg
     1981 aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg
     2041 tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc
     2101 gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt
     2161 ttactggatg aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc
     2221 tttttagaca tctaatcttt tctg
//
LOCUS       DAYLZO010000376         2244 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.244, whole genome shotgun sequence.
ACCESSION   DAYLZO010000376 DAYLZO010000000
VERSION     DAYLZO010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.244"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            <1..755
                     /locus_tag="ACGVJG_003405"
     CDS             <1..755
                     /locus_tag="ACGVJG_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFJ6559256.1"
                     /translation="EPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDY
                     NKIDLTLLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCC
                     NEFWNVTPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKF
                     IERYISEDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNI
                     TKYTRDMYKKYTGKTGCLDSTYAADIEERREQ"
     gene            752..1294
                     /gene="sat4"
                     /locus_tag="ACGVJG_003406"
     CDS             752..1294
                     /gene="sat4"
                     /locus_tag="ACGVJG_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFJ6559257.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1387..2181
                     /locus_tag="ACGVJG_003407"
                     /note="aph(3')-IIIa"
     CDS             1387..2181
                     /locus_tag="ACGVJG_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFJ6559258.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          749 a          355 c          532 g          608 t
ORIGIN      
        1 tagaaccgtt tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc
       61 aaaagccgga ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc
      121 tatttgatga ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt
      181 acctaaaggg cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg
      241 acatagttcc gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg
      301 attgctgcaa tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg
      361 aaattttatt tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga
      421 tatcatggaa ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt
      481 ttattgaaag gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg
      541 attcctatga aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat
      601 ccggtgaggt ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa
      661 aatataccag ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat
      721 atgccgctga tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct
      781 gaaagatatc gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta
      841 tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
      901 ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac
      961 tgataagata atatatcttt actaccaaga cgataaatgc gtcggaaaag ttaaactgcg
     1021 aaaaaattgg aaccggtacg cttatataga agatatcgcc gtatgtaagg atttcagggg
     1081 gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt
     1141 gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa
     1201 ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     1261 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt
     1321 cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa
     1381 taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc
     1441 tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat
     1501 gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa
     1561 cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac
     1621 tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc
     1681 tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc
     1741 atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc
     1801 cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac
     1861 tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa
     1921 aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg
     1981 aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg
     2041 tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc
     2101 gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt
     2161 ttactggatg aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc
     2221 tttttagaca tctaatcttt tctg
//
LOCUS       DAYLZO010000377         1860 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.261, whole genome shotgun sequence.
ACCESSION   DAYLZO010000377 DAYLZO010000000
VERSION     DAYLZO010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.261"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            22..933
                     /locus_tag="ACGVJG_003408"
     CDS             22..933
                     /locus_tag="ACGVJG_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFJ6559259.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1086..1571
                     /gene="vanZ-A"
                     /locus_tag="ACGVJG_003409"
     CDS             1086..1571
                     /gene="vanZ-A"
                     /locus_tag="ACGVJG_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFJ6559260.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          656 a          257 c          355 g          592 t
ORIGIN      
        1 tagttaagga ggagactgaa aatgaagaag ttgttttttt tattgttatt gttattctta
       61 atatacttag gttatgacta cgttaatgaa gcactgtttt ctcaggaaaa agtcgaattt
      121 caaaattatg atcaaaatcc caaagaacat ttagaaaata gtgggacttc tgaaaatacc
      181 caagagaaaa caattacaga agaacaggtt tatcaaggaa atctgctatt aatcaatagt
      241 aaatatcctg ttcgccaaga aagtgtgaag tcagatatcg tgaatttatc taaacatgac
      301 gaattaataa atggatacgg gttgcttgat agtaatattt atatgtcaaa agaaatagca
      361 caaaaatttt cagagatggt caatgatgct gtaaagggtg gcgttagtca ttttattatt
      421 aatagtggct atcgagactt tgatgagcaa agtgtgcttt accaagaaat gggggctgag
      481 tatgccttac cagcaggtta tagtgagcat aattcaggtt tatcactaga tgtaggatca
      541 agcttgacga aaatggaacg agcccctgaa ggaaagtgga tagaagaaaa tgcttggaaa
      601 tacgggttca ttttacgtta tccagaggac aaaacagagt taacaggaat tcaatatgaa
      661 ccatggcata ttcgctatgt tggtttacca catagtgcga ttatgaaaga aaagaatttc
      721 gttctcgagg aatatatgga ttacctaaaa gaagaaaaaa ccatttctgt tagtgtaaat
      781 ggggaaaaat atgagatctt ttattatcct gttactaaaa ataccaccat tcatgtgccg
      841 actaatcttc gttatgagat atcaggaaac aatatagacg gtgtaattgt gacagtgttt
      901 cccggatcaa cacatactaa ttcaaggagg taaggatggc ggaatgaaac caacgaaatt
      961 aatgaacagc attattgtac tagcactttt ggggtaacgt tagcttttta atttaaaacc
     1021 cacgttaact aggacattgc tatactaatg atacaactta aacaaaagaa ttagaggaaa
     1081 ttatattggg aaaaatatta tctagaggat tgctagcttt atatttagtg acactaatct
     1141 ggttagtgtt attcaaatta caatacaata ttttatcagt atttaattat catcaaagaa
     1201 gtcttaactt gactccattt actgctactg ggaatttcag agagatgata gataatgtta
     1261 taatctttat tccatttggc ttgcttttga atgtcaattt taaagaaatc ggatttttac
     1321 ctaagtttgc ttttgtactg gttttaagtc ttacttttga aataattcaa tttatcttcg
     1381 ctattggagc gacagacata acagatgtaa ttacaaatac tgttggaggc tttcttggac
     1441 tgaaattata tggtttaagc aataagcata tgaatcaaaa aaaattagac agagttatta
     1501 tttttgtagg tatacttttg ctcgtattat tgctcgttta ccgtacccat ttaagaataa
     1561 attacgtgta agatgtctaa atcaagcaat ctgatctttc atacacataa agatattgaa
     1621 tgaattggat tagatggaaa acgggatgtg gggaaactcg cccgtaggtg tgaagtgagg
     1681 ggaaaaccgg tgataaagta aaaagcttac ctaacactat agtaacaaag aaagcccaat
     1741 tatcaatttt agtgctgagg aattggtctc tttaataaat ttccttaacg ttgtaaatcc
     1801 gcattttcct gacggtaccc ccaaactaag atttcataga tagattttct gataacttta
//
LOCUS       DAYLZO010000378         1509 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.283, whole genome shotgun sequence.
ACCESSION   DAYLZO010000378 DAYLZO010000000
VERSION     DAYLZO010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.283"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            complement(39..341)
                     /locus_tag="ACGVJG_003410"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACGVJG_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACGVJG_003411"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACGVJG_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACGVJG_003412"
     CDS             complement(1055..1345)
                     /locus_tag="ACGVJG_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFJ6559261.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          483 a          295 c          207 g          524 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagatt
//
LOCUS       DAYLZO010000379          745 bp    DNA     linear   BCT 16-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate PR36742-1-C1
            SAMN29128726-rid22351153.guided.349, whole genome shotgun sequence.
ACCESSION   DAYLZO010000379 DAYLZO010000000
VERSION     DAYLZO010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29128726
            Sequence Read Archive: SRR19758519
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002258511.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002258511.1
            Genome Coverage       :: 322x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/15/2024 22:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,413
            CDSs (total)                      :: 3,356
            Genes (coding)                    :: 3,268
            CDSs (with protein)               :: 3,268
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29128726-rid22351153.guided.349"
                     /isolate="PR36742-1-C1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019"
                     /collected_by="University of Michigan"
     gene            132..680
                     /gene="aac(6')"
                     /locus_tag="ACGVJG_003413"
     CDS             132..680
                     /gene="aac(6')"
                     /locus_tag="ACGVJG_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFJ6559262.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          272 a          110 c          166 g          197 t
ORIGIN      
        1 ttttttattt tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa
       61 gatttttgag cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag
      121 ggggaagacg tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc
      181 tttctgattt actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag
      241 tagaagaaat gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag
      301 taggatttat tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag
      361 ttgtagaaag ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa
      421 aagaagtagc ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg
      481 gaacaacgtt aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc
      541 agaaccttcg tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg
      601 tcttaccaaa tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc
      661 ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat
      721 aagaaggaat ttacgaaaat tactt
//
